LOCUS       DAEBXD010000001        89421 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEBXD010000001 DAEBXD010000000
VERSION     DAEBXD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89421
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.001"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(86..469)
                     /gene="rplQ"
                     /locus_tag="KJL94_000001"
     CDS             complement(86..469)
                     /gene="rplQ"
                     /locus_tag="KJL94_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBD4408526.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(510..1499)
                     /gene="rpoA"
                     /locus_tag="KJL94_000002"
     CDS             complement(510..1499)
                     /gene="rpoA"
                     /locus_tag="KJL94_000002"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBD4408527.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(1525..2145)
                     /gene="rpsD"
                     /locus_tag="KJL94_000003"
     CDS             complement(1525..2145)
                     /gene="rpsD"
                     /locus_tag="KJL94_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBD4408528.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(2179..2568)
                     /gene="rpsK"
                     /locus_tag="KJL94_000004"
     CDS             complement(2179..2568)
                     /gene="rpsK"
                     /locus_tag="KJL94_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBD4408529.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2585..2941)
                     /gene="rpsM"
                     /locus_tag="KJL94_000005"
     CDS             complement(2585..2941)
                     /gene="rpsM"
                     /locus_tag="KJL94_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBD4408530.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3088..3204)
                     /gene="rpmJ"
                     /locus_tag="KJL94_000006"
     CDS             complement(3088..3204)
                     /gene="rpmJ"
                     /locus_tag="KJL94_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBD4408531.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(3236..4567)
                     /gene="secY"
                     /locus_tag="KJL94_000007"
     CDS             complement(3236..4567)
                     /gene="secY"
                     /locus_tag="KJL94_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBD4408532.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(4575..5009)
                     /gene="rplO"
                     /locus_tag="KJL94_000008"
     CDS             complement(4575..5009)
                     /gene="rplO"
                     /locus_tag="KJL94_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBD4408533.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(5013..5192)
                     /gene="rpmD"
                     /locus_tag="KJL94_000009"
     CDS             complement(5013..5192)
                     /gene="rpmD"
                     /locus_tag="KJL94_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBD4408534.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(5196..5699)
                     /gene="rpsE"
                     /locus_tag="KJL94_000010"
     CDS             complement(5196..5699)
                     /gene="rpsE"
                     /locus_tag="KJL94_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBD4408535.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(5714..6067)
                     /gene="rplR"
                     /locus_tag="KJL94_000011"
     CDS             complement(5714..6067)
                     /gene="rplR"
                     /locus_tag="KJL94_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBD4408536.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(6077..6610)
                     /gene="rplF"
                     /locus_tag="KJL94_000012"
     CDS             complement(6077..6610)
                     /gene="rplF"
                     /locus_tag="KJL94_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBD4408537.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(6623..7015)
                     /gene="rpsH"
                     /locus_tag="KJL94_000013"
     CDS             complement(6623..7015)
                     /gene="rpsH"
                     /locus_tag="KJL94_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBD4408538.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7049..7354)
                     /gene="rpsN"
                     /locus_tag="KJL94_000014"
     CDS             complement(7049..7354)
                     /gene="rpsN"
                     /locus_tag="KJL94_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBD4408539.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7369..7908)
                     /gene="rplE"
                     /locus_tag="KJL94_000015"
     CDS             complement(7369..7908)
                     /gene="rplE"
                     /locus_tag="KJL94_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBD4408540.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(7923..8237)
                     /gene="rplX"
                     /locus_tag="KJL94_000016"
     CDS             complement(7923..8237)
                     /gene="rplX"
                     /locus_tag="KJL94_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBD4408541.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(8248..8619)
                     /gene="rplN"
                     /locus_tag="KJL94_000017"
     CDS             complement(8248..8619)
                     /gene="rplN"
                     /locus_tag="KJL94_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBD4408542.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8784..9038)
                     /gene="rpsQ"
                     /locus_tag="KJL94_000018"
     CDS             complement(8784..9038)
                     /gene="rpsQ"
                     /locus_tag="KJL94_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBD4408543.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(9038..9229)
                     /gene="rpmC"
                     /locus_tag="KJL94_000019"
     CDS             complement(9038..9229)
                     /gene="rpmC"
                     /locus_tag="KJL94_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBD4408544.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(9229..9639)
                     /gene="rplP"
                     /locus_tag="KJL94_000020"
     CDS             complement(9229..9639)
                     /gene="rplP"
                     /locus_tag="KJL94_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBD4408545.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(9652..10353)
                     /gene="rpsC"
                     /locus_tag="KJL94_000021"
     CDS             complement(9652..10353)
                     /gene="rpsC"
                     /locus_tag="KJL94_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBD4408546.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(10371..10703)
                     /gene="rplV"
                     /locus_tag="KJL94_000022"
     CDS             complement(10371..10703)
                     /gene="rplV"
                     /locus_tag="KJL94_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBD4408547.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(10718..10996)
                     /gene="rpsS"
                     /locus_tag="KJL94_000023"
     CDS             complement(10718..10996)
                     /gene="rpsS"
                     /locus_tag="KJL94_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBD4408548.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(11013..11834)
                     /gene="rplB"
                     /locus_tag="KJL94_000024"
     CDS             complement(11013..11834)
                     /gene="rplB"
                     /locus_tag="KJL94_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBD4408549.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(11852..12154)
                     /gene="rplW"
                     /locus_tag="KJL94_000025"
     CDS             complement(11852..12154)
                     /gene="rplW"
                     /locus_tag="KJL94_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBD4408550.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(12151..12756)
                     /gene="rplD"
                     /locus_tag="KJL94_000026"
     CDS             complement(12151..12756)
                     /gene="rplD"
                     /locus_tag="KJL94_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBD4408551.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(12767..13396)
                     /gene="rplC"
                     /locus_tag="KJL94_000027"
     CDS             complement(12767..13396)
                     /gene="rplC"
                     /locus_tag="KJL94_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBD4408552.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(13429..13740)
                     /gene="rpsJ"
                     /locus_tag="KJL94_000028"
     CDS             complement(13429..13740)
                     /gene="rpsJ"
                     /locus_tag="KJL94_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBD4408553.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            14121..14588
                     /locus_tag="KJL94_000029"
     CDS             14121..14588
                     /locus_tag="KJL94_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD4408554.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="KJL94_000030"
     CDS             complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="KJL94_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBD4408555.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="KJL94_000031"
     CDS             complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="KJL94_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBD4408556.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="KJL94_000032"
     CDS             complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="KJL94_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4408557.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LG"
     gene            complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="KJL94_000033"
     CDS             complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="KJL94_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBD4408558.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="KJL94_000034"
     CDS             complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="KJL94_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBD4408559.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="KJL94_000035"
     CDS             complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="KJL94_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBD4408560.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="KJL94_000036"
     CDS             complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="KJL94_000036"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBD4408561.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="KJL94_000037"
     CDS             complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="KJL94_000037"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBD4408562.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="KJL94_000038"
     CDS             complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="KJL94_000038"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBD4408563.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(21084..21806)
                     /locus_tag="KJL94_000039"
     CDS             complement(21084..21806)
                     /locus_tag="KJL94_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4408564.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="KJL94_000040"
     CDS             complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="KJL94_000040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD4408565.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            23006..23224
                     /locus_tag="KJL94_000041"
     CDS             23006..23224
                     /locus_tag="KJL94_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBD4408566.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="KJL94_000042"
     CDS             complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="KJL94_000042"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD4408567.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(23958..24158)
                     /locus_tag="KJL94_000043"
     CDS             complement(23958..24158)
                     /locus_tag="KJL94_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBD4408568.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(24168..25905)
                     /gene="kefB"
                     /locus_tag="KJL94_000044"
                     /pseudo
     CDS             complement(24168..25905)
                     /gene="kefB"
                     /locus_tag="KJL94_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(25905..26456)
                     /gene="kefG"
                     /locus_tag="KJL94_000045"
     CDS             complement(25905..26456)
                     /gene="kefG"
                     /locus_tag="KJL94_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBD4408569.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            26587..28500
                     /locus_tag="KJL94_000046"
     CDS             26587..28500
                     /locus_tag="KJL94_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4408570.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            28500..29522
                     /locus_tag="KJL94_000047"
     CDS             28500..29522
                     /locus_tag="KJL94_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD4408571.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            29516..29734
                     /locus_tag="KJL94_000048"
     CDS             29516..29734
                     /locus_tag="KJL94_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBD4408572.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            29788..30657
                     /locus_tag="KJL94_000049"
     CDS             29788..30657
                     /locus_tag="KJL94_000049"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBD4408573.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(30712..31116)
                     /locus_tag="KJL94_000050"
     CDS             complement(30712..31116)
                     /locus_tag="KJL94_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBD4408574.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            31418..32050
                     /gene="crp"
                     /locus_tag="KJL94_000051"
     CDS             31418..32050
                     /gene="crp"
                     /locus_tag="KJL94_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBD4408575.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            32101..34191
                     /locus_tag="KJL94_000052"
     CDS             32101..34191
                     /locus_tag="KJL94_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD4408576.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(34258..35478)
                     /gene="argD/dapC"
                     /locus_tag="KJL94_000053"
     CDS             complement(34258..35478)
                     /gene="argD/dapC"
                     /locus_tag="KJL94_000053"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBD4408577.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(35564..36127)
                     /gene="pabA"
                     /locus_tag="KJL94_000054"
     CDS             complement(35564..36127)
                     /gene="pabA"
                     /locus_tag="KJL94_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBD4408578.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(36159..36761)
                     /locus_tag="KJL94_000055"
     CDS             complement(36159..36761)
                     /locus_tag="KJL94_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBD4408579.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(36751..36918)
                     /locus_tag="KJL94_000056"
     CDS             complement(36751..36918)
                     /locus_tag="KJL94_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBD4408580.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(37023..37604)
                     /gene="ppiA"
                     /locus_tag="KJL94_000057"
                     /gene_synonym="rotA"
     CDS             complement(37023..37604)
                     /gene="ppiA"
                     /locus_tag="KJL94_000057"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBD4408581.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            37875..39056
                     /gene="tsgA"
                     /locus_tag="KJL94_000058"
     CDS             37875..39056
                     /gene="tsgA"
                     /locus_tag="KJL94_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBD4408582.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            39318..41861
                     /gene="nirB"
                     /locus_tag="KJL94_000059"
     CDS             39318..41861
                     /gene="nirB"
                     /locus_tag="KJL94_000059"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBD4408583.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            41858..42184
                     /gene="nirD"
                     /locus_tag="KJL94_000060"
     CDS             41858..42184
                     /gene="nirD"
                     /locus_tag="KJL94_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBD4408584.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            42310..43116
                     /gene="nirC"
                     /locus_tag="KJL94_000061"
     CDS             42310..43116
                     /gene="nirC"
                     /locus_tag="KJL94_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBD4408585.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            43135..44508
                     /gene="cobA"
                     /locus_tag="KJL94_000062"
     CDS             43135..44508
                     /gene="cobA"
                     /locus_tag="KJL94_000062"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD4408586.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            44755..44922
                     /locus_tag="KJL94_000063"
     CDS             44755..44922
                     /locus_tag="KJL94_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBD4408587.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            45217..46554
                     /gene="frlA"
                     /locus_tag="KJL94_000064"
                     /pseudo
     CDS             45217..46554
                     /gene="frlA"
                     /locus_tag="KJL94_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            46575..47597
                     /gene="frlB"
                     /locus_tag="KJL94_000065"
     CDS             46575..47597
                     /gene="frlB"
                     /locus_tag="KJL94_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBD4408588.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            47647..48477
                     /gene="frlC"
                     /locus_tag="KJL94_000066"
     CDS             47647..48477
                     /gene="frlC"
                     /locus_tag="KJL94_000066"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBD4408589.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            48474..49259
                     /gene="frlD"
                     /locus_tag="KJL94_000067"
     CDS             48474..49259
                     /gene="frlD"
                     /locus_tag="KJL94_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBD4408590.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            49359..50090
                     /gene="phoB"
                     /locus_tag="KJL94_000068"
     CDS             49359..50090
                     /gene="phoB"
                     /locus_tag="KJL94_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBD4408591.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(50250..51335)
                     /locus_tag="KJL94_000069"
     CDS             complement(50250..51335)
                     /locus_tag="KJL94_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD4408592.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(51347..52651)
                     /locus_tag="KJL94_000070"
     CDS             complement(51347..52651)
                     /locus_tag="KJL94_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBD4408593.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(52663..53016)
                     /locus_tag="KJL94_000071"
     CDS             complement(52663..53016)
                     /locus_tag="KJL94_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBD4408594.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(53027..53905)
                     /locus_tag="KJL94_000072"
     CDS             complement(53027..53905)
                     /locus_tag="KJL94_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBD4408595.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(53902..55128)
                     /locus_tag="KJL94_000073"
     CDS             complement(53902..55128)
                     /locus_tag="KJL94_000073"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD4408596.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(55129..56292)
                     /locus_tag="KJL94_000074"
     CDS             complement(55129..56292)
                     /locus_tag="KJL94_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBD4408597.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(56376..56738)
                     /locus_tag="KJL94_000075"
     CDS             complement(56376..56738)
                     /locus_tag="KJL94_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBD4408598.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(56755..57660)
                     /locus_tag="KJL94_000076"
     CDS             complement(56755..57660)
                     /locus_tag="KJL94_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408599.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(57930..58934)
                     /gene="trpS"
                     /locus_tag="KJL94_000077"
     CDS             complement(57930..58934)
                     /gene="trpS"
                     /locus_tag="KJL94_000077"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBD4408600.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(58927..59685)
                     /gene="gph"
                     /locus_tag="KJL94_000078"
     CDS             complement(58927..59685)
                     /gene="gph"
                     /locus_tag="KJL94_000078"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBD4408601.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(59678..60355)
                     /gene="rpe"
                     /locus_tag="KJL94_000079"
     CDS             complement(59678..60355)
                     /gene="rpe"
                     /locus_tag="KJL94_000079"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBD4408602.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(60373..61209)
                     /gene="dam"
                     /locus_tag="KJL94_000080"
     CDS             complement(60373..61209)
                     /gene="dam"
                     /locus_tag="KJL94_000080"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD4408603.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(61316..62602)
                     /gene="damX"
                     /locus_tag="KJL94_000081"
     CDS             complement(61316..62602)
                     /gene="damX"
                     /locus_tag="KJL94_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBD4408604.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(62694..63782)
                     /gene="aroB"
                     /locus_tag="KJL94_000082"
     CDS             complement(62694..63782)
                     /gene="aroB"
                     /locus_tag="KJL94_000082"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBD4408605.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(63839..64360)
                     /gene="aroK"
                     /locus_tag="KJL94_000083"
     CDS             complement(63839..64360)
                     /gene="aroK"
                     /locus_tag="KJL94_000083"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBD4408606.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(64761..65999)
                     /gene="hofQ"
                     /locus_tag="KJL94_000084"
     CDS             complement(64761..65999)
                     /gene="hofQ"
                     /locus_tag="KJL94_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBD4408607.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(65911..66315)
                     /gene="hofP"
                     /locus_tag="KJL94_000085"
     CDS             complement(65911..66315)
                     /gene="hofP"
                     /locus_tag="KJL94_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBD4408608.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(66305..66745)
                     /gene="hofO"
                     /locus_tag="KJL94_000086"
     CDS             complement(66305..66745)
                     /gene="hofO"
                     /locus_tag="KJL94_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBD4408609.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(66729..67268)
                     /gene="hofN"
                     /locus_tag="KJL94_000087"
     CDS             complement(66729..67268)
                     /gene="hofN"
                     /locus_tag="KJL94_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBD4408610.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(67268..68047)
                     /gene="hofM"
                     /locus_tag="KJL94_000088"
     CDS             complement(67268..68047)
                     /gene="hofM"
                     /locus_tag="KJL94_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBD4408611.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            68167..70719
                     /gene="mrcA"
                     /locus_tag="KJL94_000089"
     CDS             68167..70719
                     /gene="mrcA"
                     /locus_tag="KJL94_000089"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBD4408612.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(70884..71444)
                     /gene="nudE"
                     /locus_tag="KJL94_000090"
     CDS             complement(70884..71444)
                     /gene="nudE"
                     /locus_tag="KJL94_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBD4408613.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            71764..73899
                     /gene="igaA"
                     /locus_tag="KJL94_000091"
     CDS             71764..73899
                     /gene="igaA"
                     /locus_tag="KJL94_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBD4408614.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            73964..74632
                     /gene="yrfG"
                     /locus_tag="KJL94_000092"
     CDS             73964..74632
                     /gene="yrfG"
                     /locus_tag="KJL94_000092"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBD4408615.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            74643..75044
                     /gene="hslR"
                     /locus_tag="KJL94_000093"
     CDS             74643..75044
                     /gene="hslR"
                     /locus_tag="KJL94_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBD4408616.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            75069..75938
                     /gene="hslO"
                     /locus_tag="KJL94_000094"
     CDS             75069..75938
                     /gene="hslO"
                     /locus_tag="KJL94_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBD4408617.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(76082..77806)
                     /locus_tag="KJL94_000095"
     CDS             complement(76082..77806)
                     /locus_tag="KJL94_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBD4408618.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            78185..79807
                     /gene="pckA"
                     /locus_tag="KJL94_000096"
     CDS             78185..79807
                     /gene="pckA"
                     /locus_tag="KJL94_000096"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBD4408619.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(79883..81235)
                     /gene="envZ"
                     /locus_tag="KJL94_000097"
     CDS             complement(79883..81235)
                     /gene="envZ"
                     /locus_tag="KJL94_000097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBD4408620.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(81232..81951)
                     /gene="ompR"
                     /locus_tag="KJL94_000098"
     CDS             complement(81232..81951)
                     /gene="ompR"
                     /locus_tag="KJL94_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBD4408621.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            82179..82655
                     /gene="greB"
                     /locus_tag="KJL94_000099"
     CDS             82179..82655
                     /gene="greB"
                     /locus_tag="KJL94_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBD4408622.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            82752..85073
                     /locus_tag="KJL94_000100"
     CDS             82752..85073
                     /locus_tag="KJL94_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBD4408623.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            85511..85738
                     /gene="feoA"
                     /locus_tag="KJL94_000101"
     CDS             85511..85738
                     /gene="feoA"
                     /locus_tag="KJL94_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBD4408624.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            85755..88076
                     /gene="feoB"
                     /locus_tag="KJL94_000102"
     CDS             85755..88076
                     /gene="feoB"
                     /locus_tag="KJL94_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBD4408625.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            88076..88312
                     /gene="feoC"
                     /locus_tag="KJL94_000103"
     CDS             88076..88312
                     /gene="feoC"
                     /locus_tag="KJL94_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBD4408626.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            88515..89402
                     /gene="rpnA"
                     /locus_tag="KJL94_000104"
     CDS             88515..89402
                     /gene="rpnA"
                     /locus_tag="KJL94_000104"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBD4408627.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        21540 a        24170 c        22342 g        21369 t
ORIGIN      
        1 aaaagagtac agctattgta gataagtggg gatactacgg gtataaaaaa acccgccggg
       61 gcgggttttt ttacattgct tcagattact ctgcagcagc ttctgctttc tctgaacgat
      121 caaccagctc gatgtaagcc atcggcgcgt tgtcgcctgc acggaagcca cacttcagaa
      181 tacgagtgta accaccggca cggctcgcga aacgcgggcc cagttcgtta aacagttttg
      241 ccacgatctc gttatcacga gtacgggcga atgccagacg acgattagca acgctatcag
      301 tcttggcaag agtaatcagc ggctcaacta cgcggcgcag ctctttcgct ttaggcagag
      361 tcgtcttgat gatttcatga cgaaccagtg aacctgccat attgcggaac atagcctggc
      421 gatggctgct gttgcggttc agttgacgac cactcttacg atggcgcatg accttatcct
      481 tctcagtaaa accttaacct gtgatccggt tactcgtcag cgatgcttgc cggtggccag
      541 ttttccaagc gcatgcccag agacagtcca cgggaagcca gcacgtcttt aatctcagta
      601 agagattttt taccaaggtt aggcgtttta aggagctcaa cctcggtacg ctgtaccaga
      661 tcaccgatat agtggatagc ttctgcttta aggcagttag cagagcggac agtcaattcc
      721 agatcgtcaa cagggcgcag caggatcgga tcgaactctg gtttctcttc tttcacttca
      781 ggctgacgta catcacgtaa gtcaacgaaa gcttccagtt gttcagccag aatggttgcc
      841 gcacgacgaa tcgcctcttc aggatcgatt gtgccgttgg tttccatttc gatgaccagc
      901 ttgtccaggt cggtacgctg ttctacacgc gctgcttcaa cattgtaggc aatacgctcc
      961 acagggctgt agcatgcgtc gaccagcaga cggccgattg ggcgctcatc ttcttccgaa
     1021 tgaattcggg tagaagccgg cacataacca cgaccgcgct gaactttgat acgcatgcta
     1081 atagacgcgt tctcatcggt caggtggcag atcacgtgct gcggcttgac gatttcgaca
     1141 tcaccgtcgt gggtgatatc ggctgcagtc acagggccaa tgccagattt attcaaggta
     1201 agaataactt catctttgcc ctgaactctc accgccagcc ctttcaggtt gagcaggatt
     1261 tccaggatat cttcctgaac gccttctttg gtgctgtact catgtagtac accatcaatc
     1321 tcaacctcgg tcaccgcgca acccggcatc gatgagagca gaatacggcg cagtgcgtta
     1381 cccagagtat ggccaaagcc acgctctaaa ggctcaaggg tcaccttggc gtgcgtcgaa
     1441 ctcacttgct cgatatcaac caggcgcggt tttagaaact ctgtcacaga accctgcatt
     1501 gtgtcctctc tttggtacta agctttactt ggagtaaagc tcgacgatca ggtgttcgtt
     1561 aatgtccgca gacagatcag aacgctccgg cttacgctta aacgtacctt ccatcttgcc
     1621 agcatcaact tccagccaag ttggcttttc acgctgctca gccagctcca gagcggcttt
     1681 cacgcgagac tgcttcttcg ctttctcacg aatgcttaca acgtcattcg gactaacctg
     1741 ataagaagcg atgttaacaa cacgaccgtt taccataatt gctttatggc taaccagctg
     1801 acgtgcttct gcacgagtgg caccgaagcc catacggtat acaacgttgt ccagacgacc
     1861 ttccagcaga gccaacaggt tttcaccggt gttgcctttc agacgtgctg cttctttgta
     1921 gtagttacgg aactgacgct ccagcacacc atagatacgg cgaacttttt gcttttcacg
     1981 caactgcaca ccatagtcag acagacgcgg tttacgcgca ccgtgctggc caggagcttg
     2041 ttcaatttta cacttggtat cgatcgcgcg aacgccagac ttaaggaata agtcggtgcc
     2101 ctcacgacgg ctcagcttga gcttaggacc caaatatctt gccattttct ttctccaaca
     2161 aacctggaaa acgaggcgtt atacgcgacg ttttttcggc ggacgacaac cgttatgagg
     2221 gatcggagtc acatcagtaa tgttagtgat gcggaaacct gcggcgttca gagcacgaat
     2281 agtagattcg cggcctggac ccggaccttt aaccataact tccagattct tgatgccgta
     2341 ttctttcacg gcgtcagcgc aacgctctgc tgcaacctga gctgcaaacg gagtggattt
     2401 gcgagaacca cggaaaccgg aaccaccggc tgttgcccaa cccaacgcgt taccctgacg
     2461 atcagtgata gtcacgatgg tgttgttgaa agaagcatgg atatgagcca cgccgtcaga
     2521 gacttgtttt cttacacgtt tacgtgcacg aattggtgcc tttgccatta ttcaatcacc
     2581 ccgattattt cttgatcggt ttgcgcggac ccttacgggt acgtgcgttg gtcttggtac
     2641 gctgaccgcg aaccgggaga ccacgacgat gacgcaaacc gcgatagcaa ccaagatcca
     2701 tcaggcgctt gatgctcatg ctgatttcac ggcgcagatc accttcaacg acaaatttgg
     2761 caacttcgtc acgcagcgtg tcgatttgtc cttcagacag ctcactgatc ttaacatctt
     2821 cagcgatacc cgctgcagcc aggatggctt tagaacgggt cttgccgacg ccataaatcg
     2881 aagttaatgc gattacggca tgcttatgat caggaatgtt aatgcctgct atacgggcca
     2941 ctatgcactc ctactattta atatgtacgt tccatgctga aaagcccgtt ttcaggatac
     3001 tcaaatggaa acgtacagac atacaaaaga ttggctggct aatctagcca gctcaaccca
     3061 actttgcaag aaaaatatgc gaaaaaatca gccttggcgc tgtttatgct tcggctcggc
     3121 actgcaaatc acacggatga caccatcacg cttaacgatt ttgcagttac ggcataattt
     3181 cttgacggaa gcacgaactt tcatttttac tctccgtaac ttctcgggcg accaattatc
     3241 ggccgtagcc tttcaggttc gccttcttca atgcagactc atactgactg gacatcatca
     3301 gagtttgcac ttgagccata aagtccataa tcacgacaac aacgataagc agtgaggtcc
     3361 caccgaagta gaacggtact ttcattgcat cacgcatgaa ctccgggatc aggcagataa
     3421 aggtaatata cagcgcacca accaaggtca ggcgggtcat tactttatcg atatacttcg
     3481 ccgtttgctc tcccggacga attcctggta caaatgcacc ggacttcttc aggttatctg
     3541 ctgtttcacg cgggttgaaa accaacgccg tgtagaagaa acagaagaag atgattgcag
     3601 acgcatagag taacacataa agcggttgcc caggctgcaa atacagcgaa attgttgtca
     3661 gccagttcca accagtaccg cccccgaacc atgacgcgat ggtcgccggg aacagaataa
     3721 tactggaagc gaagattgcc gggattaccc ccgccatatt cactttcagc ggtaaatgtg
     3781 tgctctgtgc agcatagaca cgacgacctt gctgacgttt cgcgtagttt accacaatgc
     3841 ggcgttgacc acgctcaaca aacacaacaa agaacgtcac tgcaaatact aatactgcaa
     3901 ccaacagcaa cacgaggaag tgcaggtcgc cttgacgcgc ttgctcgata gtatgggcaa
     3961 tggctggcgg gagtcccgcg acaataccgg cgaagataat gattgaaata ccgttgccga
     4021 tacctcgttc agtgatctgt tcgcccaacc acatcaggaa catcgttcct gtgaccagac
     4081 ttacaacagc ggtgaagtag aatgcaaagc ccgggttaat caccaggcct tgcataccag
     4141 gcatattcgg cagaccggta gcaataccga tcgactggaa tattgccagc accagagtac
     4201 cgtaacgggt gtactggctg atcttacgac gaccagactc cccttctttc ttaatttctg
     4261 ccaacgttgg gtgaaccacc gtcagcagct ggataatgat cgacgccgaa atatacggca
     4321 tgatccccag agcaaagata gaagcacggc tgagagcacc accagagaac atgttaaaca
     4381 tctcaatgat ggtgcctcgc tgttgctcaa gcagtttggc aagtacagcg gcatcaatac
     4441 cagggatcgg aataaaagag ccaatacgga acacaatcag cgcaccgata acaaacagca
     4501 gtctgcgttt cagctcgcct aagccacctt tggcactttg aaaatctaat cccggttgtt
     4561 tagccatctg ctacttattc ctcgatttta ccgccagcag cttcgatagc agcacgagcg
     4621 cctttagtaa cacgcaggcc acgaacagtt accggagtcg ttacttcgcc agccaggatc
     4681 actttcgcga actcgatctg gataccgata atgttagccg ctttcagcgt gttcaggtct
     4741 acaacaccgc cttctacttt agccaggtca gatagacgaa cttcggctgt aatcgctgct
     4801 ttacgagaag tgaagccgaa tttcggcaga cgacggtaca gaggcatctg accaccctcg
     4861 aaaccgcgac gtacgccacc gccagaacga gacttctgac ctttgtgacc acgaccaccg
     4921 gttttaccga ggccagaacc gataccacga cccaggcgtt tacccgcctt tttggagcct
     4981 tcggccggag acagagtatt taaacgcatc tcttactcct caactttaac catgaaggaa
     5041 accgcgttga tcataccgcg aatagcagga gtatcctcgc gctctacggt gtgaccaata
     5101 cgacgcagac ccaagccaag cagcgttgcc ttgtgtttcg gcagacgacc gattgcactg
     5161 cgggtttgag taattttaat agtctttgcc atggtttatt tccccagaat ttcttcaacg
     5221 gatttaccac gcttggcagc gaccatttct ggagaattca tattttccag gccatcaata
     5281 gttgcacgaa ccacgttgat cgggttggtg gaaccatatg ctttagccag aacgttatga
     5341 accccagcga cttccagaac ggcgcgcatt gcaccaccgg cgatgatacc ggtaccttcg
     5401 gaagccggct gcatgaatac gcgagaaccc gtgtgaacac ctttaacagg gtgttgtaga
     5461 gtgccgttat tcagcgcgac gttaatcata ttgcgacggg ctttttccat cgctttctgg
     5521 atcgctgctg gaacttcacg cgctttaccg taaccaaaac caacgcgacc gttaccatcg
     5581 ccaactacag tcagagctgt gaaggagaaa atacgaccac ctttaacggt tttagatacg
     5641 cggtttaccg cgatcagctt ttcctgcagt tcgccagctt gtttttcgat gtgagccatc
     5701 ttacacctct accttagaac tgaaggccag cttcacgggc agcatctgcc agtgcctgga
     5761 cacgaccatg atattggaac ccggaacggt caaaggatac atctttgatg cctttttcca
     5821 gagcgcgttc agcgacagct ttacccacag ctgcagccgc gtctttgtta ccggtgtact
     5881 tcagttgttc agcgatagct ttttctacag tagaagcagc taccagaact tcagaaccgt
     5941 tcggtgcaat tacctgtgcg taaatgtgac gcggggtacg atgtaccacc aggcgagttg
     6001 cgcccagctc ctggagcttg cggcgtgcgc gggtcgcacg acggatacga gcagatttct
     6061 tatccatagt gttaccttac ttcttcttag cctctttggt acgcacgact tcgtcggcgt
     6121 aacgaacacc cttgcctttg taaggctcag gacgacggta ggcgcgcaga tccgctgcaa
     6181 cctggccgat cacctgctta tcagcgcctt tcagcacgat ttcagtctga gtcggacatt
     6241 cagcagtgat acccgcaggc agctgatggt caacaggatg agagaaaccc agagacaggt
     6301 taatcacatt gcctttaacc gctgcacggt aacctacacc aaccagctgc agcttcttag
     6361 tgaagccttc ggtaacaccg ataaccattg agttcagcag ggcacgcgcg gtaccagcct
     6421 gtgcccaacc gtctgcgtaa ccatcacgcg gaccgaaggt cagggtatta tctgcatgtt
     6481 taacttcaac agcatcgttg agagtacgag tcagctcgcc gtttttacct ttgatcgtaa
     6541 taacctgacc gttgattttt acgtcaacgc cggcaggaac aacgaccggt gctttagcaa
     6601 cacgagacat tttttcctcc gattaggcta cgtagcagat aatttcgcca ccaagaccag
     6661 cctggcgcgc tgcacgatca gtcataacac ctttagaggt agaaacaact gcgataccca
     6721 gacccgccat aactttcggc agctcatctt tacgtttata gatgcgcaga cctgggcggc
     6781 tgacacgctg aatgctttct acaacagctt tgccctggaa atacttcaga gtaagttcca
     6841 gttcaggctt ggtgtcgcct tcaactttaa aatcttcaat aaaaccttct tccttcagca
     6901 cgttggcgat tgccactttc agcttggagg aaggcatggt gaccgcagct ttgttcgcgg
     6961 cctgaccgtt acggatacgg gtcagcatat ccgcgatcgg atcttgcatg ctcatctgtc
     7021 tttactcccg tgattcaatt ggtgacaatt accagctagc ctttttcaga cccgggattt
     7081 caccgcgcat agcggcttca cggaccttaa tacggctcaa cccgaacttc cgcaggaaac
     7141 catgcggacg acctgtttga cggcagcggt tacgctgacg agacgggctg gaatcacgcg
     7201 gcagagtctg cagcttgaga acagcgttcc aacgatcttc gtcggaagcg ttcacatcag
     7261 agatgatcgc tttcagttca gcgcgtttcg cgaagtattt atcagctaaa gctacgcgtt
     7321 ttacttcgcg tgctttcatt gattgcttag ccatttagta accctacctt acttgcggaa
     7381 cgggaagtca aaggcagcca gcagagcgcg gccttcttcg tcagatttcg cagtagtggt
     7441 aatggtaata tccaaaccac gaacgcggtc gactttatcg tagtcgattt ctgggaagat
     7501 gatctgctca cggacaccca tgctgtagtt accacgaccg tcgaaagact tagcggacag
     7561 gccacggaag tcacggatac gaggtacagc aatagtgatc aggcgctcaa agaactccca
     7621 catgcgttcg ccacgcagag ttactttaca gccgatcgga tagccctgac ggattttgaa
     7681 gcctgcaaca gatttgcgtg ctttggtgat cagcggtttt tgaccggaga ttgctgccag
     7741 gtctgctgct gcgttatcca gcagtttttt gtcagcgatc gcttcaccaa cacccatgtt
     7801 cagggtgatc ttctcgaccc gagggacttg catgacagaa ttgtagttaa actcagtcat
     7861 gagtttttta actacttcgt ctttgtagta atcatgcagt ttcgccatcg tactactcca
     7921 aattacttga tagtttcgct gttagacttg aagaaacgga cttttttgcc gtcttcgaat
     7981 ctaaagccta cacggtcagc cttgccggtt gccgcattga agattgctac gttggaaacc
     8041 tgaatagcgg cttctttttc aacgatgcca cccggttggt tcagggccgg aaccggcttc
     8101 tgatgtttct taaccaggtt gataccttca acaatgacct tgccggaaga caggacattc
     8161 ttaactttac cgcgtttacc tttatcttta ccggttaaca cgataacttc gtcatcacga
     8221 cggattttcg ctgccatgat tcgctcctta gagtacttct ggtgccagag agataatttt
     8281 catgaacttc tcactacgaa gctcacgagt taccggccca aaaatacgcg taccgatagg
     8341 ctgctcgctg ttgttgttca gaagaacaca agcattacca tcgaagcgaa tgacagaacc
     8401 gtccgggcga cgaacaccct tcttggtgcg caccactacc gccttcagca catcaccttt
     8461 tttgacctta ccacgcggaa ttgcttcttt gatggtgatc ttgatgatgt cgcctacgcc
     8521 tgcgtagcga cggtgcgagc cacccagaac cttgatacac attacgcgac gtgcaccgga
     8581 gttgtcggcg acgttcagca tagtctgttc ttggatcatt ttagtgctcc gctaatgtca
     8641 actactactg agacccgaaa atcaggtcgt taaaaatccc catatcgagg gcgcggcatt
     8701 ataacaccgc ttcaaggata tgggtagaaa aaataaacgg ctcatttctg agccgtttat
     8761 tcgtatcgag agagtgtact gtattacaga accgctttct ctacaacgcg aaccagcgtc
     8821 caggatttag tcttggacag cggacggcat tcgcggattt caaccacgtc accgataccg
     8881 cattcgttgt tctcgtcatg tacgtgcagt ttggtcgtac gcttgatgaa tttaccgtag
     8941 atcgggtgtt tcacaaaacg ttcgatagca acaacaatgg atttctccat tttgtcgcta
     9001 acaacgcgac cttgcagagt acggatttta tcggtcatta cgcacccgcc ttctcgttca
     9061 gtaaagtctt aacgcgtgcg acatcgcgac gcacttgctt caacaggtga gactgttgca
     9121 gctggccact tgcagcctgc atacgcaggt tgaactgctc acgcagcagg ttcagcagct
     9181 cggtgttcag ctcttcaatg ctcttctcac gcagctcttt tgctttcatt acatcaccgt
     9241 cttagttaca aaggtggttt taatcggcag tttcgctgct gccagcttga atgcttcacg
     9301 ggccagctct tccggaacac cgtccatttc atacaggact ttacccggct gaatcaaggc
     9361 aacccaatac tccacgttac ctttaccttt acccatacgc actgccagcg gcttttcagt
     9421 gatcggtttg tccgggaaca cacggatcca gatcttacct tgacgcttaa ctgcacgggt
     9481 catagcacga cgtgctgctt cgatctgacg ggcagtcaga cgaccacggc caacagcttt
     9541 cagaccgaag ctgccgaagc taacatccgt accctgcgcc agaccgcggt tacggccttt
     9601 gtgcatttta cggaattttg tacgctttgg ttgtaacatc agcgacgctc cttatttacg
     9661 gcctttacgc tgctgctttt taggctgagc agccggtttt tccggttgtt caacagcagc
     9721 cataccaccc aggatctcgc ctttgaagat ccacacttta acgccgatta caccgtaagt
     9781 ggtgtgcgct tcagaggtgt tgtagtcgat gtcagcacgc agagtgtgca gcggtacgcg
     9841 accttcgcgg taccattcgg tacgtgcgat ttccgcgccg cccagacggc cgctaacttc
     9901 aactttaata cctttagcgc ccagacgcat tgcgttctgt acagcacgct tcatagcacg
     9961 acggaacata acgcgacgtt ccagctgaga agtgatgctg tcagcaacca gttttgcgtc
    10021 cagttcaggc ttacgaactt cggcgatgtt gatctgtgca ggaacgccag cgatgtccgc
    10081 tacgacctta cgcagttttt ctacgtcttc acctttttta ccgataacga tacccgggcg
    10141 agcagtgtga atggttacac ggatgctctt agccggacgc tcgataacga tacgagatac
    10201 ggacgcttta gccagttcct tagtcaggta ctgacgtact ttaaaatcgc tgtccaggtt
    10261 gtcagcgaat tctttggtgt tcgcaaacca agtagagttc catggtttta caatacccaa
    10321 gcgaatacca ttaggatgta ctttctgacc cattgctagt ctccagagtc tcagcgatcg
    10381 gacacaacca cagtgatgtg gctggtgcgc ttcaggatgc gatctgcacg accttttgca
    10441 cgcggcataa tgcgcttcat gctcgggcct tcgtctacga aaattttcgt aactttcaga
    10501 tcgtcaatgt cagcgccatc gttgtgttca gcgttagcaa tggcagattc cagaactttc
    10561 ttgacctgta cagccgcttt cttgttggtg taggtcaaaa tatccagagc ctgcgacact
    10621 ttcttaccgc gaatcaggtc agcaacaagg cgaaccttct gagcagaaga acgagcatgg
    10681 cgatgtttag caatagtttc catctcttcc tcctacctta tttcttcttc gcttttttat
    10741 cagcagcgtg gccgcgataa gtacgagtcg gtgcgaattc acccagtttg tgaccgacca
    10801 tttcgtcggt tacaaatacc ggaacgtgct gacgaccatt atggacagcg atggtcaaac
    10861 cgatcatgtt aggaaagatc gttgaacgac gggaccaagt gcgcaggggc ttcttgtctc
    10921 cgctttccac cgctttctct accttcttca gcaagtgcag gtcaataaaa ggacctttct
    10981 tgagagaacg tggcatggct tatcctctaa aattatttgc tacggcgacg tacgatgaat
    11041 ttatcagtac gcttgttgct gcgggtcttc ttacctttgg tctgaacgcc ccacggagtt
    11101 accgggtgct taccaaagtt acgaccttca ccaccaccat gtgggtggtc taccgggttc
    11161 atcgcggtac cgcgaacggt cggacgaaca ccacgccagc gtgcagcacc tgctttaccc
    11221 agaacgcgca gcatatgctc agcattgcca acttcgccca gagttgcacg gcagtctgct
    11281 tctactttac gcatttcacc agaacgcaga cgcagggtga cataagcacc atcacgagca
    11341 acgatctgaa cgtaagtacc agcggaacgt gccagctgac cgcctttacc tggtttcatt
    11401 tctacgttat gaacagtaga accaaccggg atgttgcgca tcggcagggt gttacctggt
    11461 ttgattgcag catcaacgcc agactgaatc tggtcgccag ctttcaggcc tttaggggcc
    11521 aggatgtaac ggcgttcacc gtctttgtac agaaccagcg cgatgttcgc ggaacggttc
    11581 ggatcgtact caagacgttc aacaactgcc gggataccgt ctttgttgcg tttgaagtca
    11641 acaatacggt aagcctgctt gtggccacca ccgatatgac gagtggtgat acggccattg
    11701 ttgttacgac caccggattt gctgtttttt tccagcaacg gagcaaaagg tttgcccttg
    11761 tgcagctcag ggttaaccac tttaactacg tggcgacgac ccggagatgt cggtttacat
    11821 ttaacaactg ccattgtatt actcctccga cttactcagc gccgccaacg aagtccagat
    11881 tctggccttc tttcagggtg acgtaagctt ttttccagtc gctacgacga ccgatacgct
    11941 gtccgtgacg tttaactttc cctttaacta ccagggtgtt aacgacttcg acttcgactt
    12001 caaacagttt ctgcacagca gctttgattt ctgctttggt cgcgtctttc gcaactttga
    12061 gtacgatggt gttggatttt tccatcgcag tagacgcttt ttcagaaacg tgcggtgcac
    12121 gcagcacctt cagcagacgt tcttcacgaa tcatgccagc atctcctcaa cttgcttaac
    12181 agcatcagca gtcattacga ctttgtcgaa ggcgatcagg ctaaccgggt cgataccagt
    12241 tgcatcgcgt acgtcaacct tctgcaggtt gcgcgcagcc aggaacaggt tttcgtccag
    12301 ctcaccggtg atgatcagca catcttccag agccatgtct ttcagtttct gtgccagcag
    12361 cttagttttc ggcgcttcta cagagaactt ctcgacaacg atcagacgat cctgacgtac
    12421 cagttcggac aggatgcttt tcagcgcgcc gcggtacatc ttcttgttaa ctttttgact
    12481 gtggtcctgc ggacgagcag caaaggtcac gccaccagaa cgccagatcg ggctcttgat
    12541 agaaccagaa cgcgcacggc cggtgccttt ctggcgccac ggttttttac cggaaccagt
    12601 tacttcagca cgagtcttct gagcacgagt accctgacga gcaccagctg cataagcaac
    12661 aacaacctgg tgaaccagcg cttcgttgaa atcacgaccg aaggtagttt cggaaacagt
    12721 cagcgcgctc tgcgcgtctt tcaatactaa ttccattgct atctccttac gccttcacag
    12781 ctggtttaac gatcaggtcg ctaccggttg cacccgggac agcaccttta accagcagca
    12841 ggttgcgctc agcgtcaacg cgtactacgt caaggctctg aacggttaca cgttcgttac
    12901 ccatctgacc tgccattttc ttgcctttga acactttgcc cggagtctgg ttctgaccga
    12961 tagaacccgg aacgcggtga gacaaggagt taccgtgagt agcgtcctgg gtacggaagt
    13021 tccagcgctt aacggtacct gcgaaacctt tacctttaga ggtgccagtt acgtcaactt
    13081 ttttaacgtc agcaaacagt tcaacgctaa tgctctgacc tacagtgaac tcttcgcctt
    13141 cagccaggcg gaattcccac agaccacggc cagcttctac gccagcttta gcgaagtggc
    13201 cagcttcagg cttggtcaca cggttagctt ttttagcacc ggtggtcacc tgaatagcac
    13261 ggtagccatc gttagccagg tctttaacct gagtaacgcg gtttgcttca acttcgatta
    13321 cggttactgg gatagaaacg ccgtcttctg tgaagatacg ggtcataccc acttttttac
    13381 cgactaaacc aatcattgtt tcaacctctc aatcgctcaa tgacctgatt aacccaggct
    13441 gatctgcacg tctacaccgg cagccagatc cagacgcatc agagcatcaa cggttttctc
    13501 ggttggctca acgatgtcaa ccagacgcaa gtgagtacgg atttcgtact gatcgcgcgc
    13561 gtctttgttg acgtgcgggg agatcagaac agtgaagcgc tctttgcgtg tcggcagcgg
    13621 gatcggacca cggacctgcg caccagtgcg cttggcagtc tcgacgattt ccgcggttgc
    13681 ttgatcgatc agacgatgat caaacgcttt caggcggata cggattcttt ggttctgcat
    13741 gagaccagag ctccaattat tttataaacg aaaatgatta ctcctcagac ccattacgat
    13801 tgatgggaga gtgtaaccgt tcttacgtag ctccccgatt gggagcattg ttaggtagcc
    13861 aaattcggct aactgaggtt cagattgaac ctgctgtcaa ctacgacaag cccgcgcatt
    13921 atacgcaaat ctgagcctga cgcaagcatc gggtagaaat taatcgcaac gcgcagattg
    13981 cgagctgcat tccacgaaat ttgtaaggtt gtgaagatag cgtttttcac cggaatcagg
    14041 ttaacaaatc agcgcgctct ctctgttgct gagcatccgg agttattcga tggtgatact
    14101 ccaattacag aggatgtttt atggacacta cccttccctt tctaattttg tacgcttgtt
    14161 tatcagccct actttttttc tgggatgcaa agcatggctt gttacctgac agatttacgt
    14221 gcccgttact ctggtcaggt cttttgttct atcaagtttg tcatcctgat ggtttagccg
    14281 atgcgttatg gggggcaatt gttggttacg gcacattcgc tgtcatttac tgggggtatc
    14341 gcatactgcg acataaagaa ggattaggct atggtgatgt gaagtttctc gccgcccttg
    14401 gcgcctggca ttcatgggcc ttcttaccac gattggtttt ccttgctgcc tcattcgctt
    14461 gcggagccgt agttattggt ctgctcatga gaggaaaaga atcattaaaa aacccgctgc
    14521 cttttgggcc atttctggcg gctgcgggtt tcgttatagg ctgggatagt ttgctgatag
    14581 gccgttaatc tttaacttta atttgtgatt gcaggtaatt ttgtaggccg attttaccga
    14641 tgagatccag ttcagtttcg agccagtcga tatgaccttc ttcatcagca aggatctcga
    14701 tcatcatatc gcggctaaca tagtcatgaa cgctgtcggc atatgcgata gcctcacgca
    14761 gatccttcgc gccttccagt tctaatcgca ggtcagattg cagcatctcc tcgacatctt
    14821 caccaatccc cagtttgcct aaatcctgta agttggggat tccttctaaa aacaaaatac
    14881 gctcgatata tttatcagcg tgtttcatct catcgatgga ttcatggtat tcgacgtcat
    14941 tgagacgcat caggccccag tttttaaaca tccgggcatg gagaaaatac tgattgattg
    15001 cgacaagctc atttcccaat agtttattga gataatttat gattttaaca tcacctttca
    15061 ttttatagtc cctccgcttc cactattaga gcgtagatgg ggctagcagg atgtcaaaaa
    15121 ataagcgtgg gcttcaggca atctctttaa attctggcat ctgcatcaac tcgtcctgca
    15181 tcacttcgcg cgcggcgcga atgcacttac cgcattgatt tcccacagga ataaacttac
    15241 gcaattgttg gaaagattgc ggatgaaact gacggactgc ctggcgaatt tttttgtcac
    15301 ttacaccatt acacaaacaa acgtacatga ttgctcccgt tcaaattctg cacaaagtgt
    15361 aaatgagaat ggttatgatt acaatagtac agttttattt gcgccatagt tcagcgagca
    15421 aaattacgac taaatatttc gagctcaaat tacgggcagc aaaaagggcg ccgaagcgcc
    15481 cttttcaatt caaaactaat taacgtgtaa ttagcccaga actttagcaa caacgcccgc
    15541 gccaacggta cggccgcctt cacggattgc gaaacgcaga ccgtcgtcca tcgcgatcgg
    15601 gtggatcagg gtaacaacca ttttgatgtt gtcgcccggc attaccatct ctacgccttc
    15661 cggcagttcg atggtaccag tcacgtcagt agtacggaag tagaactgcg gacggtagcc
    15721 tttgaagaac ggagtatgac ggccgccttc atctttggac agaatgtaca cttcagattc
    15781 gaacttggtg tgcggcttga tggtgcccgg cttagccagt acctgaccac gttcgatttc
    15841 ttcacgtttg ataccacgca gcagaacacc tacgttctca ccagcacggc cttcgtccag
    15901 cagtttgcgg aacatttcaa cgccagtaca ggtagacttc tgagtctctt tgataccaac
    15961 gatttcaact tcttcaccaa ctttgatgat accgcgttct acacgaccgg taacaacggt
    16021 accacgaccg gagatggaga atacgtcttc gatcggcagc aggaacggct tgtcaatcgc
    16081 acgctctggt tccggaatat aagaatccag gaagccagcc agttccagga ttttcgcttc
    16141 ccactctgcg tcgccttcca gcgctttcag agcagaacca cgaacgatcg gagtgtcgtc
    16201 gcccgggaag tcgtactgag acagaagttc acgaacttcc atttcaacca gttccagcag
    16261 ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg atgatgtacg gaacgcctac
    16321 ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc gggccgtcag tcgcagcaac
    16381 taccaggatc gcgccgtcca tctgagcagc accggtgatc atgtttttaa catagtcggc
    16441 gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg gtgtcatatt caacgtgaga
    16501 agtgttgatg gtgataccac gagctttttc ttccggcgcg ttatcgatct ggtcgaatgc
    16561 acgagcagca ccgccgtagg ttttagccag tacggtggtg attgcagcgg tcagagtagt
    16621 tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg tgcggttttg tacgttcaaa
    16681 tttttcttta gacacggcta tattccttac tatagtgctc tccccttcag gagagagcac
    16741 gggactttgg tattaaccct taggcttatt taccacgggc ttcaattacg gcctgagcaa
    16801 cgttactcgg cgcttcatca tacttcagga attccatagt gtatgatgca cgacctttgg
    16861 tcagagaacg cagctgagtt gcgtatccga acatttcaga cagcggtact tcagcgtgga
    16921 tcttaacgcc agtaacttca gattcctgac ctttgagcat accacgacga cggctcaagt
    16981 caccgataac gtcaccggtg ttctcttccg gagtttctac ttcaaccttc atgatcggct
    17041 caagcagaac tggtttcgct ttcttaaagc cttctttaaa ggcgatagaa gcagccagtt
    17101 taaacgccag ttcagaggag tcaacgtcat ggtaagaacc gaagtgcaga cgaataccca
    17161 tgtctactac cgggtagcct gccagcggac ctgctttcag ctgttcctgg atacctttat
    17221 caacggccgg gatgtattcg ccagggatta caccaccttt aatgtcgttg atgaactcgt
    17281 agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc gataacaaca tgaccatact
    17341 gaccacgacc accagactgt ttcgcgtgtt taccttcaac atcggtaact ttctggcgga
    17401 tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc ttcaacgttg aattcacgct
    17461 tcatacggtc aacgatgatg tcgaggtgca gttcgcccat acccgcgatg atggtctggt
    17521 tagattcttc gtcagtccat acacggaaag acgggtcttc tttagccaga cggcccagag
    17581 ccagacccat tttttcctgg tcagctttgg ttttcggttc aactgcgatg gagattaccg
    17641 gctcagggaa ttccatacgt tccagaatga tcggcgcatc cgggtcacac agggtgtcac
    17701 cagtggttac gtctttcaga ccgatagcag cagcgatgtc gcccgcgcga acttctttga
    17761 tctcttcacg tttgttagcg tgcatctgaa cgatacgacc gaaacgctca cgtgcagctt
    17821 tcacggagtt cagtacggta tcaccagagt taaccacacc ggagtaaaca cggaagaagg
    17881 tcaggttacc aacaaacggg tcggtagcga ttttgaacgc cagtgcagag aacggctcgt
    17941 catcacttgc gtgacgttca gccggagtgt ctttaccgtc gtccaggata ccgttgatcg
    18001 caggtacgtc aaccggggat ggcaggtaat caattaccgc atccagcatc gcctgaacac
    18061 ctttgttctt gaacgcagaa ccacaggtta ccaggatgat ttcgttgttc agaacgcgct
    18121 gacgcagagc acctttgatt tctgcttcag tcagttcttc accacccagg tatttttcca
    18181 tcagctcttc agaagcttca gctgcggatt cgatcaggtt ctggtgccat tcgttagcca
    18241 gttcaaccat gtctgccggg atatcttcgt attcgaaggt tacgccctgg tcagcgtcgt
    18301 tccagttgat agctttcatt ttcaccaggt caacaacacc ggtgaaatgt tcttcagcac
    18361 caatcgccag ctgcagcgga accgggttcg cgcccagacg ggttttgatc tggttaacaa
    18421 ctttcaggaa gttcgcaccc acgcggtcca ttttgttaac gaacgcaatg cgcggaactt
    18481 tatatttgtt tgcctgacgc catacggttt cagactgcgg ctgaacacca ccaactgcgc
    18541 agtaaaccat taccgcacca tcgagaacac gcatggaacg ttctacttcg attgtgaagt
    18601 caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg ctcatactgc ttagccatac
    18661 cagaccagaa tgcagtagtc gcagcggaag tgatggtaat accacgttcc tgctcctgct
    18721 ccatccagtc catggttgca gcgccgtcat gaacttcacc gattttatgg tttacaccgg
    18781 tgtagaacag aatacgttcg gtagtagtgg ttttaccggc gtcgatgtgc gcactgatac
    18841 cgatgttacg gtagcgtgcg atgggtgttg tacgagccat ttgtttcctc gtttatcttt
    18901 taggcgttca atttaagtag cccaaagcgg gctgcttact ggaagcgccc gcctggtgac
    18961 taaaactccg aagggattac caacggtagt gtgcgaacgc cttgttggct tcggccatac
    19021 ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt ttctgcagca tcagaaagtt
    19081 cgttcgccag gcgcagagcc atggatttat caccgcgttt acgagcagct tcaacgatcc
    19141 aacgcattgc cagagcatta cgacgaaccg gacggacttc aactggtacc tgataagtag
    19201 aaccaccaac gcggcgagac ttaacttcta cagtcgggcg cacgttttcg agagctactt
    19261 cgaatgcttc cagttcagat ttaccagagc gctgagccag ggtctccagc gcgctgtata
    19321 cgatagattc agcagtagat tttttaccat ctaccatcag gatatttaca aatttagcca
    19381 gcagttctga tccgaacttc ggatccggca gaattttacg ctgaccaatg acgcgacgac
    19441 gtggcatgga aatactccgt tgttaattca ggattgtcca aaactctacg agtttagttt
    19501 gacatttaag ttaaaacgtt tggccttact taacggagaa ccattaagcc ttaggacgct
    19561 tcacgccata cttggaacga gcctgcttac ggtctttaac gccggagcag tcaagcgcac
    19621 cacgtacggt gtggtaacga acacccggga ggtctttaac acgaccgcca cggatcagga
    19681 tcacggagtg ctcctgcagg ttgtgacctt caccaccgat gtaggaagtc acttcgaaac
    19741 cgttagtcag acgaacacgg catactttac gcagcgcgga gttcggtttt ttaggagtgg
    19801 tagtatatac acgagtacat acgccacgtt tttgcgggca tgcttccagc gcaggcacgt
    19861 tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag ctggttaact gttgccatta
    19921 aatagctcct ggttttagct tttgcttcgt aaacacgtaa taaaacgtcc tcacacaata
    19981 tgaggacgcc gaattttagg gcgatgccga aaaggtgtca agaaatatac aacgatcccg
    20041 ccatcaccag gccatctggc tggagtgctt aaccgtaagt ctgacgaaat cagtatagtc
    20101 aatgggaatg atgtcgttca aaatttgaac agtcaaaccg cgggcaataa ggtcttcgtt
    20161 cagggcatag accttaatgg gggcattacg cagactttca aggtagcggt taccgtcaac
    20221 tgcggcagtt acgccatctt gcaataatag cagttcgtct ccttcactga gcagacgcag
    20281 cagcgcagca aaatccgtca gccagggtga acgatgtaac gtgtgcagca taaacgcctc
    20341 aaaacctcaa aataacatcg tagttggcga gttcgcggcg taaggcatct gcttcgagcg
    20401 gcgtggcttc gacaacaaag ggtgtctgcg gatctaaccc gcgttcgcgc agtgaagccg
    20461 cacaaaccca gcactgttca atgtcgtaca gactcaacaa tttaaaagtg gcaatgtaat
    20521 cacgcgccag cactgcatcg ggcttttgtc ctgacagcag ctgaaaaacg ccatcagcaa
    20581 taaagaagac ggccagattg tcagttaatg cggaagttgc cagtaaagca tctaaacctt
    20641 cccgaccagc agctgtacca tgaggtgcag tagaaaaaac aaacgcaatt cgtttcatca
    20701 gaactgtacc accctgtcac aggtcagcga ggcttccgcc agcgcgccaa gtccgcttaa
    20761 ggtaaatccc ggctgaaggt ttgacgaagc cagccccagc cttcctgctt cagtttcatc
    20821 gacgataccg cggcgtaacg ctgccgctac gcagatattc agcgccacac catgttgcgc
    20881 attcaactgt tgccagccgc gcacaaggtc aaattcgtcg cttgccggtg aggtcagttg
    20941 gttagcgtta tagacccctt cccgatagaa aaagacgctg cttaacttat ggccttctgc
    21001 tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt tgcgtaccgt atgctggccc
    21061 ggtcaccacg atggcaaaac gcattactta tcttgcccct gaaaatcacc gctcttgaac
    21121 tggcggatgt agagatagac agtgtgtttg gagatgttca ggcggtcagc aacctggttg
    21181 atcgcatctt taatatcgaa gatccctttc tcgtagagat tcagcacgat ctgacgattt
    21241 ttggcgttat tagaaacatt gcgatcggca ttcacttctt cgatggtgaa ctccagcgtt
    21301 tgggtaacca gatcttcaac agaagaggca aagttgacgc ttgaaccgac atccggggtt
    21361 tctggcggca caaaagtgct cataatctgc gagaagggaa catcaagatt catattgatg
    21421 cacagcagac caattacacg ctgttcgcgg ttacgaatcg cgatagtcag ggacttcatt
    21481 aatacgccgc ttttggcgcg agtaaagtag catttagaaa cgctgctatc cgctcccgtc
    21541 atatcgtgca gcatacgtag cgccaggtca gtaattggcg aaccaatctt ccggcctgta
    21601 tgttcaccgt tagcaatgcg aatggctgaa cattttagat cctgcaaaga gtgcaaaacg
    21661 atttcacagt gggagccaat aagcatcgct aacccgtcca ccaccgcttc gtaggatttc
    21721 agaatatcaa aatcggtctg gtcgaaagga cgttgatcca gtaaatccaa ctcactggtt
    21781 tcgttggtta aaagcgacct ggacatgaaa aaaaacactc cttttcagga gcctgtcgtt
    21841 aacttttcag ggcaggctca ttaatgatgc gggtaactaa attaatacag cggaggttcc
    21901 gctttccagc actaattata tccggcctgt aataaaaaaa ccgccgcctg gtcaggcggc
    21961 ggttcttaat gcttattttt tagcagaatc tgcggctttc gcatcagctt ccggctttgc
    22021 atcagccttc ggcgctggtt tcacatccag cagctctacg tcaaacacta gggtagaatt
    22081 cggtgggatc cccggaacac ccgctttgcc gtaagccagt tctggtggaa taaccagttt
    22141 gatcttaccg cctttcttga tgttcttcag accttctgtc caacccggga taacaccgtc
    22201 cagacggaaa gaaagcggtt caccacgggt gtaagagttg tcgaactctt taccgtcgat
    22261 cagcgtacct ttgtagttca ctacaacagt atcgctgtct ttcggtgctt cgcctttacc
    22321 ggcttctact acctgataaa ccagaccagt tgaagaggtt ttcacacctt tctctttggc
    22381 aaatttctcg cggtactctt taccttttgc ttcgttatca gccgcgtctt tttccatctt
    22441 cgcctgagca gaagacttca cgcgagcttc gaatgcttgc agagtctgtt cgatctcttg
    22501 gtcggagagt ttgctcttat cagcaaatgc atcctgaaca ccagcgatca gctgatcttt
    22561 atccagtttg atgcccagtt tttcttgttc tttaagagag ttttccatgt aacgacccag
    22621 cgaagcaccc agtgcataag ctgatttctg atcgtcattt ttgaacgatg ctttgctgtc
    22681 tgcagttgta gcaggttttg cagcttcagc agcaaaagtg attggtgcat gcagggcaac
    22741 ggccattgtg gtcgccagta gcgttacttt aaacagtgat ttcatccata tctccaggat
    22801 cggggcatct caccccaggg ttaactatta tcagaagggt actataaagc gttgtcgaac
    22861 aaatctacat acagacacgc cctattatca tctattttca gactcttttt gtttaaatta
    22921 gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa agttaagtag aatccgcggc
    22981 gaccattcga caaaagaggt gaatcatgca ggatttatca ttggaagcac gcctggcaga
    23041 gctggagagc cgactggctt ttcaggaaat caccattgaa gaactgaacg tcacggtgac
    23101 cgctcatgaa atggagatgg cgaaactgcg cgatcatctg cgtctgctga ccgagaagtt
    23161 aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa gaaacgccac cgccacatta
    23221 ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc accttttcgg tattagtggc
    23281 aaccacaacc gccgttgcct ttaccgccac agcagccttc gccaccgtgt tcatgaccgt
    23341 gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg atcgtggtga tgatcgtgcg
    23401 cgccgtgaac gtggccatga gccagttctt cttcggtcgc ttcgcgaatc gccacaactt
    23461 caacgttgaa tttcaggttc tgaccggcca gcatgtggtt accatcaacc actacgtgat
    23521 cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg gtcagtttca gccaggaaac
    23581 gcatacctac ctgcagttca tcaacgccca taaatacgtc tttaggaaca cgttgcacta
    23641 ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac agcgacatca aatttgtcgc
    23701 caacttcatg accttccagc gccgtttcca ggccagagat cagggaaccg tgaccatgca
    23761 ggtagtccag cggcgcactc accggagact catcaaccaa cacaccgtct tctgtacgta
    23821 cctgataggc caggctgacc accaggtctt ttgctacttt catgatatct cctgagcatg
    23881 ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc gtgtactcac tagcttaaaa
    23941 aaactcggcg catatcgcta gtccggatga aaaatcccga tcacttgctc atctttgtga
    24001 acgtgatcgc gggcttcttt gtctgcttct cgcatctgat gtccgcactt aacacattca
    24061 acaatatcaa tattattttc gcgccacatc gccattgaat cctgcgcctg acaggccggg
    24121 cattttgcgc ccgctataaa acgttttcgg attgccatcc ttaccctcta ctcaaattca
    24181 tcccagccgt ccagctggcg tcgttcttgt tgcatttcac gctggaaaat ctcttccagt
    24241 tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat cagcatgcat cgggattaac
    24301 tcccgcagca ttcgcatatc caggcggcga aaatgcagtt gcgcccgctg tgcctgatgc
    24361 ggatgcatgc caagcgtgac cagcgtcttg cgccccagct ctaacgcact ggagaatgtt
    24421 tcacgggaaa actgcgtcac ccctgcctgt aataactcat gcgcttccac acgtccgcgc
    24481 gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc atatttccac cagcttcatg
    24541 gtgtcttccg gctcgttaca ggtaatgacg atagactcag cggcctctgc acccgcagaa
    24601 cgtaaaagat cgacctgcgt ggcgtcgccg taataaactt tgtagccata tttacgcatc
    24661 aggttaacgg cgctgatatc ccgctccagc acggtaatgc gcattttatt tgccatcagc
    24721 aaacgaccaa tcacctgacc aaaacgcccg aagcccacga caatgacctg gggtttatca
    24781 tcgttgaccc acggtttttc gtcttcttct tccggtccgt taaactggcg ggatagccat
    24841 ttatccacca gcttcatcag caacggcgcc atctggtcgc cctggaataa acgttgtgaa
    24901 gaagcggtag aaaagaggac aaaggcaaac tcaccgccct gactcaacac gccagcaaac
    24961 tgcatccgct ctgagctacg cacgccatac aatcgcgcca gcagatacag cacgagaatt
    25021 ttcaccgcca ccagcacaac cacgcttatc actacccaca acagatgggt ataaagcacc
    25081 ccgaggttga gcgacatgcc gacagagata aagaacaaac cgagcagcaa gcctttgaag
    25141 ggatcgatag ccgtttccag ttcatggcga tattcacttt ccgccagcag cacgcccgca
    25201 ataaatgtac cgagcgccat cgacagcccc agcgcatcca taaacaatgc ggaacccaac
    25261 accagcagca gcgtcgcggc ggtgaacact tcccgcacgc cagaagctgc aataaagcgg
    25321 aataccggac gcagtaaata gcgcccacca atcagcatgc cgacaaactc cagcaccttc
    25381 atgccgatct tcatccagtc gaaatgttcg tctgccgacc ccgccaacaa cggcactaac
    25441 gccagtgctg ggatcactgc cagatcctga aacagcagaa ccgaaaaccc gagctggccg
    25501 gattcgctgc gattcatccc tttctcacgc atcaattgca acgccattgc agttgaagac
    25561 atcgcaaagc caatgccacc gaccaccgcc gcctgccagg cgaaatccgt cagcatcaat
    25621 aatcccgcca gcaacgccgc gcttaacagc acctgtgccg cgcctacgcc aaaaatcgaa
    25681 cgccgcagtt gccaaagttt ggagggattc aactcaaggc cgatgataaa catcaggaat
    25741 accacgccga gttccgaaaa gtggaggatc tcatcgacgt cgctaataaa ccccagcccc
    25801 cacgggccaa ttgcaatccc cgccagcaaa tatcccaaca cagcgccaat ccccagccgc
    25861 gaagagaaac agcactcctg cgagtaaaaa atcggaacct tccatcagcg gcctcctgga
    25921 gacagcggat ttgccaacca gtcaccgtag gctctggcgt ggctcgccag ctcctttgcg
    25981 ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg gcacatgccc
    26041 gccgccagtt caaaggggcg cagcacatcg ctcatcgggt agcgattcag cgcgtcataa
    26101 cggtaagcac tttccggctc gccggtggta atcacgttac gccagtactt tcccgccagt
    26161 tggtttcctc ccggcccgct ggcaaaacca cgacttaata cccggtccag ccactctttc
    26221 agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac ctcgtgctcg
    26281 cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg cgcgtaaagg
    26341 tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg gtttgccacc
    26401 gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg agacatcatc
    26461 cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg cgcccggtgc
    26521 ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct gaacatacgg
    26581 cgccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt cctgctggat
    26641 aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa aaacggctgt
    26701 ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg cggcagctac
    26761 acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccagc gttaccgcaa
    26821 gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga agcgcagctg
    26881 cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatag caagctggat
    26941 gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg cctcggtttc
    27001 agcaatgaac aactggagcg cccggtaagt gatttctccg gtggctggcg tatgcgtctt
    27061 aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga accgactaac
    27121 cacctcgatc tcgatgccgt tatctggctg gaaaaatggt tgaagagcta tcagggcacg
    27181 ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgttgataa aattattcat
    27241 atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga agtacagcgc
    27301 gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg cgtggcacat
    27361 ctgcaaagtt atatcgatcg tttccgtgcc aaagccacca aagcgaagca ggcccagagc
    27421 cgcattaaga tgctggaacg tatggagctg attgcccccg cacacgtcga caacccgttc
    27481 cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat ggaaaaggtc
    27541 agcgcaggct atggcgatcg cattattctc gactcgatta aattgaatct ggtccccggc
    27601 tcgcgcattg gtctgttagg ccgcaacggc gcaggtaaat cgacattaat caaactgtta
    27661 gccggtgaac ttgcgccagt cagcggtgaa attggtctgg cgaaagggat caagctcggc
    27721 tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcacctat tcaacatctg
    27781 gcacgtttag cgccgcagga gctggaacaa aaactgcgtg actacctcgg cggctttggt
    27841 ttcctgggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga aaaagcccgc
    27901 ctggtgctgg cattaattgt ctggcagcgt ccgaatctgc tgctgctcga cgaaccgact
    27961 aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga gtttgaaggc
    28021 gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga cgatctctac
    28081 ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc tggaagatta tcaacagtgg
    28141 ttgagcgacg tacaaaagca ggaaaaccag accgacgaag cgccaaaaga gaacgcgaac
    28201 agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agctgcgtgc gcaaacccag
    28261 ccgctgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa cgcgcaactg
    28321 gcgcaggcgg aagagaaact cggcgacagc gaactgtatg accagagccg taaagcggag
    28381 ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga gtgcgaaatg
    28441 gcgtggctgg aagcccagga gcagcttgag cagatgctgc tggaaggcca aagcaactga
    28501 tggcgcagat aacgacgacc gatgccaatg aatttagcag cagtgctgaa ttcaccccta
    28561 tgcgcggctt tagcaattgt catctacaaa ctatgctgcc gcgtttgttt cgtcgtcagg
    28621 tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt gtcgatctcg
    28681 cgtggagtga agaccccgca caggcgaaac ataaaccgcg tttagtggtg tttcacgggc
    28741 tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg caaaagcgcg
    28801 gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac cgtatgcacc
    28861 gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg ctgcaacgcg
    28921 aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat atgctggcct
    28981 gtttgctggc aaaggaaggc aatgatctcc cggttgatgc ggcggtgatt gtctctgcgc
    29041 cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc gtttatcagc
    29101 gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc taccccggaa
    29161 cgctgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa tttgacgatc
    29221 taatcaccgc cagaattcac ggctacgccg acgctatcga ctattatcgt cagtgtagcg
    29281 ccatgccaat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc aaagacgatc
    29341 cgtttatgga tcatcaggtg atcccaaaac cggaaagtct cccctcgcag gtggagtatc
    29401 aactgactga acatggcggt catgttggct ttattggcgg tacattactt catccgcaaa
    29461 tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg aaatcatgtt
    29521 gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa gctttgtgtt
    29581 acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag tcgccgacgt
    29641 caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc acgaaacggt
    29701 caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg gacgagataa
    29761 tagtgatagc taagtcatgg agttactatg tctgccaaac atccggtcat tgcggtaaca
    29821 ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat attcgcgcag
    29881 ttaaatctgc atgcagctga ggtggaaggc gacagttttc accgttacac ccgcccggaa
    29941 atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta cttcggcccc
    30001 gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca gagcggcaaa
    30061 gggaaatctc gcaaatatct gcatacctac gacgaagccg taccctggaa tcaggtaccg
    30121 ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt ttatgaaggt
    30181 ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga cttactggtc
    30241 ggcgtagtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga caccagcgag
    30301 cgcgggcact cacgagaagc agtgatggac tcggtagtgc gttcaatgga agactatatc
    30361 aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt tcccaccgtc
    30421 gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag ctttgtggtg
    30481 atccattttc gtaatctgga agggatcgat ttcccttggc tgctggcgat gttgcaaggc
    30541 tcattcattt cccacatcaa tacgttagtg gtacctggcg gtaaaatggg gctggcaatg
    30601 gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat cgagtaactt
    30661 tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa gtcacgcggc
    30721 aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg ccactgaaca
    30781 atatttctcg gcagagagat caaccgcacg cgcaaccgct gcgtctttca ggtcgcgacc
    30841 ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt cgcggcgttc
    30901 agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca ggatcgaaac
    30961 cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg gacttggtgc
    31021 tttatcgcct gagttgccgt ccattaaaat ctggtgacca gaggcggatt cgcccagaaa
    31081 agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac tgcgtcaatt
    31141 ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct gggtcatgct
    31201 gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag gatgctacag
    31261 taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca gtacagttga
    31321 tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc ggcgttatct
    31381 ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca aaccgcaaac
    31441 agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc catccaagag
    31501 cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta aaggctctgt
    31561 ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc tgaatcaggg
    31621 tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg catgggtacg
    31681 tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc aattgattca
    31741 ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc tgcaagtcac
    31801 ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg cacagactct
    31861 gctgaacctg gcaaaacaac cagatgctat gactcacccg gacggtatgc aaatcaaaat
    31921 tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg gacgcattct
    31981 gaagatgctg gaagatcaga acctgatctc cgcacacggt aaaaccatcg tcgtttacgg
    32041 cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt gtttcccccg
    32101 atgtggcgca gactgattta tcaccccgat atcaactatg cacttcgaca aacgctggtg
    32161 ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg tctgctcttc
    32221 tccctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa acgttttttc
    32281 aaacacttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac acagctgcta
    32341 ctggtaaaag atgttcccct gccctttttg ctgaccggat taacgctggt acttggcgtc
    32401 actgctgagc tggggccatt gcacgcaaaa ttgcttcctg catcgctgct cgccgccatt
    32461 tttaccctca gtctggcggg atacatgccg gtctgggaac cattgctcat ctatgcgttg
    32521 ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg cgaacaaccg
    32581 ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga agccaaatac
    32641 agcctgctta cccagcacac cgatcctgaa aaagcgctgc caccgctgct ggtgcgccag
    32701 caaaaagcgg tcgatttaat tactcagtgc tatcagcaaa tgcatatgct ttccgcgcaa
    32761 aacaataccg attacaagcg gatgctgcgt attttccagg aggcgctgga tttgcaggaa
    32821 catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga gcgtagccat
    32881 gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg cgtgctggct
    32941 gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca aattggcgca
    33001 ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg ctactggcat
    33061 ttcagccgca tcgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg tgacttactg
    33121 gccgataaac agcggcgaat gccgttgctt ccggcactga aaagttatct gtcactaaag
    33181 tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc cagcctgatg
    33241 ggcaccgtgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt gctggtgaca
    33301 caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg aaccgtggtc
    33361 gggttaatca ttgcgggcgt agcgctgcac tttaaaattc ccgaaagtta caccctgacg
    33421 ttgatgctga ttaccaccct cgccagctac ctgatattgc gcaaaaacta cggctgggca
    33481 acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct gaacggtgag
    33541 cagtacatcc ttccacgtct tatcgatacc attattggtt gtttgattgc tttcggtggt
    33601 accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc ccatgatgct
    33661 ttagaagcct atcaggaagc gattcgcttg attctgagcg aggacccgca accaacgcca
    33721 ctggcctggc agagaatgcg ggtaaatcag gcgcataaca ctctgtataa ctcattgaat
    33781 caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa actgtgggta
    33841 acgcacagcc agtttattgt tgagcatatt aatgccatga ccacgctggc gcgggaacac
    33901 cgggcattgc cacctgaact ggcacaagag tatttacagt cttgtgaaat cgccattcag
    33961 cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt ctggcgatgc caatatcatg
    34021 gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca gcatttacag
    34081 cgggttattg gccatctgaa caccatgcac accatttcgt cgatggcatg gcgtcagcga
    34141 ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaaggcgta atacaggcta
    34201 aaccatcgga taaggcattc acgccgcatc cgacatattt tgcctgatac gtcttattta
    34261 cgccccaacc accttcgcca ccgcgtgggc gaaacgttgc atcccttcat cgatatccgc
    34321 atcttccacc accagcgacg gtgctaaacg catcacatcc ggtccggcat tcagcaccat
    34381 tacgccaacc tctgcacccg catacaggaa atcacgcgcc cgacctttgt agtgtggttt
    34441 cagctccgcg ccaattaaca gccccatacc gcgaatatcg ctaaacacat cgtactgctg
    34501 atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc cttccagcac
    34561 ttcaggggta ttgatgatat caaacgccgc gcccgctact gcacaggcca gaggattacc
    34621 gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg ccgtggtcag
    34681 catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa tatccggcgt
    34741 cacaccgtag tacctgtaag caaacaaatc gccagtccgc cccatcccgc actgcacttc
    34801 atcaaacacc aataatgcct gatgttgatc gcacagctcg cgcaagccct gcaaaaactc
    34861 tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca ccgcacaggt
    34921 gtgatcatcc atcaccgctt tcactgcatg gagatcgtta aagggaacgt ggatgatgtc
    34981 tgccggtttc ggcccaaagc cgtcggaata ttttggctgc ccacccaccg aaacggtaaa
    35041 cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga acgggctatg
    35101 acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt ccgtgccgga
    35161 gttcataaac accacgcgtt cggcaaacgt cgcctcaatc agtttgcgcc caagacgcag
    35221 cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct gggtttttaa
    35281 cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc cacccgcgaa
    35341 atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt ttaccggaat
    35401 aaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc gtgtaattgc
    35461 tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg tgaaattata
    35521 atcacaaaat atgcataaaa aatcactgaa tggcaatcgg aaatcagaga tgcaggaaat
    35581 tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac tgcacacctt
    35641 ccagatccca ctggcgatgg cgaatcccca taatctctcg ggtttcgctc caggccgtca
    35701 cgtcaaagca cgctggtaat gagtcaggtt ccaccaccag cgaatggtag cgtgtcacgg
    35761 taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc ggcgaggttt
    35821 tgccgtgcat gacctttgcg gcgcgcacaa ctttgccgcc aaatgcctgc gccattgcct
    35881 gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga ataacgtcaa
    35941 gggagatccc ggcttcatct ggcgtacagg ggccaggtga gatgacaatt ttttgtggtt
    36001 taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc acatccgccc
    36061 ccagttcaca aaagtactgg tagaggttcc aggtaaaaga atcgtagtta tctataagca
    36121 ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc cccggcttcg
    36181 cttaccactt tgctaaatat catctgcagt gcggtgagat cccccattgc cccactctga
    36241 tttgcctgat tccaggcctc tttttcgata ccctgccagc tcagttgata tccggcatga
    36301 atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg gaagggatgc
    36361 agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc tttctccagg
    36421 ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc tttttcgata
    36481 taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg cagttgccct
    36541 gcccagtcga aaatatcctg gtacagctgg cgatggatag tgcgcaaatg cggtaaaccg
    36601 cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc gtaagcggcc
    36661 tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc aaggccagga
    36721 taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc tcagctcttc
    36781 aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga cgccttcaag
    36841 acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg acttttgctt
    36901 atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac aagtataagc
    36961 ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg cgcgagaaat
    37021 cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac
    37081 ggaccaacat catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc
    37141 actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa ggcgttatcg
    37201 gcaacgttga taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg
    37261 atggtgccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg cgggtttggt
    37321 tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg
    37381 cggtgaaagg tagtgttgtt ataaaaaccg ctgttcacat aatcgacaaa gttttgcaca
    37441 gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac
    37501 aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcagaa
    37561 agagcgaaaa cagcagccat cgccgccagg gtcgatttga acatgagatt tccttaacaa
    37621 acagagcaga aaaaaagcaa gtgaagagat tctaaatagc cttcaagaac aaggccatcc
    37681 ctttacctaa ttttacgtat ctgaaatata tgtaacctga cgatcacttt ttattccgtg
    37741 atcaaaatca cctcttaaaa tgcaatttag caaccgattg caataaaaca tttaaacaga
    37801 tcataaaatc acctaaaatc gcccgctcgc ggcatatccc gctatgccat tttatttttg
    37861 aacgcaggcc aatcatgact aacagcaatc gcatcaagct cacatggatt agctttctct
    37921 cctacgcact gactggtgcg ttggttattg tcaccgggat ggtgatggga aatatcgccg
    37981 attatttcaa tctgcctgtt tccagtatga gtaacacctt caccttcctc aacgccggca
    38041 ttttaatctc tatcttcctc aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt
    38101 tacgttttgg ctttctcctg atggtgctgg cggttgccgg tttgatgttc agccacagcc
    38161 tggcgctgtt ctcgacggcg atgttcattc tcggggtggt cagcggcatt actatgtcga
    38221 ttggtacatt cctgataaca caaatgtatg aagggcgtca gcgcggttcc cgcctgttat
    38281 ttactgactc cttcttcagt atggctggga tgattttccc aatgatcgcc gcgtttctac
    38341 tggcgcgcag cattgagtgg tactgggttt atgcctgcat cgggctggtg tatgtcgcta
    38401 tttttattct gaccttcggc tgtgagttcc cggcactggg caaacgtgca ccaaaaacgg
    38461 atgctccggt agagaaagaa aagtggggga tcggcgtact gtttctctct gttgcggcgc
    38521 tgtgctacat cctcggtcag ttaggtttta tctcctgggt gcctgagtat gccaaaggcc
    38581 tgggcatgag cctgaacgac gcgggcacgc tggtgagtaa cttctggatg tcatacatgg
    38641 tcggcatgtg ggcgttcagc tttattcttc gcttctttga tttgcaacgc attctgaccg
    38701 tactggctgg tctggctgcg attctgatgt acgtctttaa caccggaaca ccagtacata
    38761 tggcgtggtc aattctcgct ctgggcttct tctccagcgc gatctatacc accatcatca
    38821 ccctgggttc acagcagacc aaagtaccgt cgccaaaact ggttaacttt gtcctgacct
    38881 gcggaaccat cggtactatg ttgacctttg tggttaccgg cccgattgtt gaacatagcg
    38941 gtccgcaggc ggcactgctt acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct
    39001 tcctgttagg tttcgtcagc cgtcaccgtc agcataacac cctgacctct cattaattgc
    39061 taatgccgga cggcactatc gtcgtccggc cttttcctct cttcccccgc tacgtgcatc
    39121 tatttctata aacccgttca ttttgtccat tttttgcaca aacatgaaat gtcagacaat
    39181 tccgtgactt aagaaaattt atacaaatca gcaatatacc ccttaaggag tatataaagg
    39241 tgaatttgat ttacatcaat aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa
    39301 aagaaatcga ggcaaaaatg agcaaagtca gactcgcaat tatcggtaac ggtatggtcg
    39361 gccatcgctt tatcgaagat cttcttgata aatctgatgc ggccaacttt gatattaccg
    39421 ttttctgtga agaaccgcgc atcgcttatg accgcgtaca cctctcgtct tacttctctc
    39481 accacaccgc cgaagagctg tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca
    39541 aagttctggt cggcgaacgc gctatcacca tcaaccgtca ggagaaggtg attcactcca
    39601 gcgccggacg taccgttttt tatgacaagc tgatcatggc aaccggttcc tacccgtgga
    39661 tcccaccaat caaaggttct gatactcagg actgctttgt ctatcgcacc attgaagacc
    39721 tcaacgccat tgaatcctgc gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc
    39781 tgttaggtct ggaagccgct ggcgcgctga aaaacttagg tattgaaacc cacgttatcg
    39841 aatttgcccc tatgctgatg gcggaacagc ttgatcagat gggtggcgag cagctgcgtc
    39901 gcaaaatcga aagtatgggc gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc
    39961 aggaaggtgt cgaagcgcgt aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg
    40021 actttatcgt cttctctacc ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc
    40081 tggacgttgc tccgcgtggg gggattgtca ttaatgattc ctgccagact tccgatccgg
    40141 atatctacgc catcggtgaa tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac
    40201 caggctacaa aatggcgcag gtcgccgttg accatattct gggtagcgaa aacgcctttg
    40261 aaggtgctga ccttagcgcc aagctgaaac tgctgggcgt agacgtaggt ggtattggtg
    40321 atgcgcacgg tcgcacgcct ggcgcacgta gctatgttta cctcgacgaa agtaaagaga
    40381 tctacaaacg cctgattgtc agcgaagaca acaaaaccct gctcggtgcg gtactggtgg
    40441 gcgataccag cgactacggc aacctgctgc aactggtgct gaacgctatc gagctgccgg
    40501 aaaacccgga ttctctgatt ctgccggcgc actcgggtag cggtaagccg tctatcggtg
    40561 ttgataaact gccggacagc gcgcaaatct gctcctgctt cgacgtcact aaaggtgatc
    40621 tgattgctgc catcaacaaa ggctgccaca cagttgcggc gctgaaagct gaaaccaaag
    40681 cgggtactgg ctgcggtggc tgtatcccgc tggtcactca ggtactgaac gcggaactgg
    40741 cgaaacaggg catcgaagtt aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg
    40801 aactgttcca tttgatccgc gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac
    40861 acggcaaagg ctacggttgt gaagtttgta aaccaaccgt cggttcgctg ctggcctcct
    40921 gctggaacga atacattctg aagccggaac atactccgct gcaggattct aacgacaact
    40981 tcctcgctaa catccagaaa gacggcacct actcggtgat cccgcgttct ccgggcggtg
    41041 aaatcacccc ggaagggctg atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca
    41101 ccaaaatcac cggctcccag cgcctggcga tgtttggcgc acagaaagac gatctgccgg
    41161 aaatctggcg tcagctgatt gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac
    41221 tgcgtatggc gaaaacctgc gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg
    41281 tcggcctcgg cgtggaactg gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga
    41341 agttcggtgt ctccggctgt acccgtgaat gttcagaagc tcagggtaaa gacgtgggta
    41401 ttatcgccac tgaaaaaggc tggaacctgt atgtttgcgg taacggcggc atgaaaccgc
    41461 gtcatgcgga tctgctggca gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc
    41521 gcttcatgat gttctacatc cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag
    41581 aaaacctcga aggcggcatc gattacctga aagcggtgat cattgacgac aaactggggc
    41641 tgaacgctca tctggaagaa gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga
    41701 ctgaaacggt caatacaccg tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca
    41761 agcgtgaccc gaacgtgcag atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt
    41821 atgaacgtat cccggtaact ctggtggagg acaacgcatg agccagtgga aagacatctg
    41881 caaaatcgat gacatcctgc ctgaaaccgg cgtctgcgca ctgttaggtg acaagcaggt
    41941 agcgattttc cgcccgtatc acagcgacca ggtgtttgcg atcagcaaca ttgatccgtt
    42001 cttcgagtcc agcgtgctat cacgcgggct gattgcggaa caccagggcg agctgtgggt
    42061 cgccagcccg ctgaaaaaac agcgttttcg cttaagtgat ggcttgtgca tggaagacga
    42121 acagttttcc gtcaaacatt acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg
    42181 ttaatgtttt aacgggaggc gcaatgcctc ccctttttgc atggtcctgt aatgaccttc
    42241 ggtatattgc aggacatttt ttaaactttt tgttttattt tttgttttta ttttttaaag
    42301 gataatcaaa tgtttacaga cactattaat aagtgtgcgg ctaatgctgc gcgcattgca
    42361 cgcctgtcgg caaataaccc gctcggcttt tgggtcagct ccgccatggc gggcgcatat
    42421 gtgggtcttg ggatcatcct gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt
    42481 ccgctggtga tgggcgcgac ctttggtatc gccttaacgc tggtgattat cgccggttct
    42541 gaactgttca ccggacacac catgttcctc acctttgggg taaaagcggg cagcatcagc
    42601 cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg gtaacctggt cggttccgtc
    42661 ttcgttgcca tgctctatag ctggggcggc ggtagcctgc tgccggtaga taccagcatc
    42721 gttcactccg tcgcgctggc taaaaccact gcaccggcaa tggtactgtt cttcaaaggt
    42781 gcattgtgta actggctggt ttgcctagca atctggatgg cgctgcgcac tgaaggggcg
    42841 gcgaaattta tcgctatctg gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac
    42901 tctatcgcta acatgacgct gttcgcgctc tcctggttcg gcaaccacag cgaagcctac
    42961 acgctggcgg gtattggtca taacctgctg tgggtgacgc tgggtaatac tttatcaggt
    43021 gccgtattca tgggattggg ttattggtat gctacgccga aagcgaatcg tccggttgcg
    43081 gacaaattta atcaaactga aacagctgcc ggttaattac taaggggttt ttacgtggat
    43141 catttgccta tattttgcca attacgcgat cgcgactgtc tgattgtcgg cggtggtgat
    43201 gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca
    43261 ttagcgttta ttccacagtt caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa
    43321 gggccatttg atgaaagcct tctcgacacc tgctggctgg cgattgcagc gacggatgat
    43381 gacacgctta accagcgcgt cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta
    43441 gttgatgcgc cgaaagccgc cagctttatt atgccgtcga ttattgaccg ctcaccgcta
    43501 atggtagcgg tctcctctgg cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa
    43561 ctcgaatcac tgctgccgtt gcatctgggc caggtggcga aatatgccgg gcaattacgc
    43621 gggcgagtga aacaacagtt cgccacgatg agtgagcgtc gccgtttctg ggagaaattg
    43681 ttcgttaacg accgcctggc gcagtcgctg gcaaacaacg atcagaaagc cattactgaa
    43741 acgaccgaac agttaatcaa cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt
    43801 gcaggtccgg gcgatgccgg gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca
    43861 gatgtggtgg tctacgaccg tctggtttct gacgatatta tgaatctggt acgccgcgat
    43921 gctgatcgcg ttttcgtcgg caaacgcgcg ggataccact gtgtaccgca ggaagagatt
    43981 aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc
    44041 gatccgttta tttttggccg tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt
    44101 ccgttctcgg tggttccggg tattaccgca gcttctggtt gctctgccta ttcgggtatt
    44161 ccactcacgc atcgcgatta tgcccagagc gtacgcttaa ttaccggaca cttaaaaacc
    44221 ggtggcgagc tggactggga aaacctggcg gcagaaaaac agacgctggt gttctatatg
    44281 gggttgaatc aggccgcgac tattcagcaa aagctgattg aacacggtat gcctggcgaa
    44341 atgccggtgg caattgtcga aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg
    44401 ctcacgcaac tcggtgaact tgctcagcaa atgaacagtc catcgctaat tattattggt
    44461 cgggttgttg gcctgcgcga taaactgaac tggttctcca atcattaatt taacccgggc
    44521 cagagaattc tggccttctt aatgactctt tttatatatt cagcaaataa aacataaccc
    44581 cttaataaat attatctgct aatgggttat attcaccgtt acagcgactt acaaaattaa
    44641 gtcattcgct cactgacgcc aattaaatat tcctgtatcg ttcttaacaa gcagtctgct
    44701 tatattcata taatcaatga atattaatta atatataata catagggaat gtaaatgaac
    44761 aaatttatta aagttgcact ggtaggtgca gtactggcta cgttaactgc atgtactggt
    44821 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct
    44881 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg
    44941 ctaacaccgg tttaattgcc cgacgccctc tggattttcc gagggcgtat ttgtttctat
    45001 gtccttcttg cccccgcccg taaaaatatt ttcactttaa attcaatttt ataactacat
    45061 ccattagcca cagcgcgatc ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca
    45121 taatatgttg ttatattcat catgcattgt catgttacct tttaaatgac tgcaaactct
    45181 cccctacaac gtattggaca agaaaaaggt atcgctatgg gaagccaaga actccaacgc
    45241 aagctcggat tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt
    45301 tttgtatctg tgggtgaagt ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg
    45361 tttgtcattg gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga actatccacc
    45421 gcttatccgg aaaatggcgc agattatgtt tatctgaaaa atgccggaag ccgaccgctg
    45481 gctttcctct ccggctgggc cagcttctgg gccaacgatg cgccgtcatt gtcgattatg
    45541 gcgctggcga ttgtcagcaa tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa
    45601 tttatcgccg ccggattaat tatcgccttt atgttgctac acctgcgctc cgttgaaggc
    45661 ggcgcagcgt ttcagacgct aattaccatc gccaaaatta tcccgttcac tatcgtcatt
    45721 ggccttggga tcttctggtt taaagcggag aattttgccg cccttaccac cactgcgatt
    45781 ggcgcaacgg gcagctttat ggcgctgctg gcggggatct ctgccaccag ttggtcgtat
    45841 accggcatgg cctctatctg ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg
    45901 ccacgagcgc tgattggttc ctgtctgctg gttctggtgc tctacaccct gctggcgctg
    45961 gtgatttccg gcctgatgcc cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac
    46021 gccctgacct agatccccgc actcggcagc accgctgggc tctttgttgc catcacggcg
    46081 atgatcgtca ttcttggttc gctttccagc tgcgtgatgt accagccgcg gctggaatac
    46141 gcgatggcga aagacaacct gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac
    46201 acgccggatg tctccatcat cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc
    46261 gatctcacca gcctgctggg ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc
    46321 ttcggctcca tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg
    46381 gctttcgggc tgatgaccac cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc
    46441 tttgtctggg caccgattcc cggccttatc tgcgccgtca tcgttattgc tactggtctg
    46501 cctgcttacg ccttctgggc gaagcgtagc cgccagctca acgctttgtc gtaatgttat
    46561 ctgaagaaaa taaaatgttg gatattgata aaagcaccgt ggactttctg gtcaccgaaa
    46621 atatggttca ggaagtggaa aaagttctca gccatgacgt tccgctggtg cacgccatcg
    46681 tggaagagat ggtgaagcgc gacattgatc gtatttattt cgttgcctgc ggctcgccac
    46741 tcaacgcggc gcaaacggcg aaacatctgg cggatcgctt ttccgatctt caggtctacg
    46801 ccatttccgg ctgggagttc tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg
    46861 tgattggcgt ttctgactac ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc
    46921 gggcctgcgg cgcactcact gcggcgttca ccaaacgcgc ggacagcccg attacctcgg
    46981 cggcggaatt tagcattgat tatcaggccg actgtatctg ggaaattcac ctgctgctct
    47041 gctacagcgt ggtgctggag atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga
    47101 tcaaaaacga tctcaagcag ttgccgaatg cgctcggtca tctggtacgc acctgggaag
    47161 aaaaaggccg ccagcttggt gaactggcca gccagtggcc gatgatttat accgttgctg
    47221 cgggtccgct gcgtccgctg ggttacaaag aaggcattgt aacgctgatg gaatttacct
    47281 ggacgcacgg ctgcgtgatt gagagcggag agttccgcca tggcccgctg gagattgtcg
    47341 aaccgggcgt tccgttcctg ttcctgctcg gcaatgatga aagtcgccac accaccgaac
    47401 gcgccattaa ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa
    47461 tttcgcaagg gctgcacccg tggctggcac cgttcctgat gttcgtgcca atggagtggc
    47521 tctgctacta cctgtctatt tacaaagatc acaacccgga tgaacgccgc tattacggtg
    47581 gtctggtgga atattaatcc ctctcccggc ccggcaacgg gccggattta cgcaaggagt
    47641 tacccgatga aaacaggtat gtttacctgc ggccaccagc ggctgcctat tgaacacgca
    47701 tttcgtgatg caagcgagct gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac
    47761 gcgttcgcgc cggacttaaa agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg
    47821 tatcagatgc cgattatcgg ttacacgcca gaaaccaacg gctatccgta taacatgatg
    47881 ctgggcgatg aacatatgcg tcgtgaaagc ctggacatga tcaagctggc gatagatatg
    47941 gcaaaagaga tgaacgcggg ttatacgctg atttctgcgg cccacgcggg ctatctcacg
    48001 ccgcctaacg ttatctgggg gcggctggca gagaacttaa gcgaactgtg tgagtacgcg
    48061 gaaaacatcg gcatggatct gatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg
    48121 tgtaatgcta atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc
    48181 atggtcgaca tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa
    48241 ctgggcgata aattacgtca tctgcatatt gtcgacagcg acggggccag cgacacgcat
    48301 tacattcctg gagaaggcaa aatgccgctg cgggaactga tgcgcgatat tattgatcgg
    48361 ggctatgagg gttactgtac ggtggagctg gtgacgatgt atatgaacga gcccagactc
    48421 tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc cggaggatga gagatgaaaa
    48481 ccctggcgac aatcggcgat aactgcgtcg atatctaccc gcaactgaat aaagcgtttt
    48541 ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg ctacggcata cagccgggat
    48601 gcattacctg ggtgggtgac gatgactacg gcacaaagct gaagcaggat ctcgcccgca
    48661 tgggcgtcga tatcagccat gtccatacga aacacggcgt taccgcacaa actcaggtgg
    48721 aactgcacga caatgatcgc gtttttggcg actacaccga aggcgtgatg gccgactttg
    48781 ccctgagtga agaggattac gcctggctgg cgcagtatga cattgtgcac gcggcaatct
    48841 ggggacatgc ggaagacgca ttcccacagc tgcacgctgc gggcaaactt accgctttcg
    48901 acttctccga caagtgggac agcccgctct ggcagacact ggtgccgcat ctcgattttg
    48961 cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct gaagatgaaa gcgattgttg
    49021 cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg
    49081 gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt tatcgacacc atgggtgccg
    49141 gagattcgtt cattgccgga ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg
    49201 cgatggcgca gggaacggcg tgcgcggcga aaaccattca gtaccacggt gcctggtagg
    49261 tataacgttg gcgtgagcat cttcacgcca acgtgctgtt acttgccgga aaacgaccct
    49321 ataatccgag taattcattc tttatttcag ggtcgattat gtcagctacg gaccgctact
    49381 ctcatcaact cctctacgct accgtccgcc agcggctgct ggatgatatc gcgcaggggg
    49441 tttaccaggc cgggcaacag atccctaccg aaaacgagct ttgtacacaa tataacgtca
    49501 gccgcattac cattcgcaaa gccatcagcg acttagtggc agacggcgta ctgatccgct
    49561 ggcagggaaa aggcaccttt gtacaaagcc agaaagttga aaacgccctg cttactgtca
    49621 gtggttttac cgattttggc gtctcacaag gcaagtcgac gaaagagaaa gtgatcgaac
    49681 aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa catccccgga aacagcgaag
    49741 tgttccatct ctgccgggtg atgtatctcg ataaagagcc gctgtttatt gatagttcat
    49801 ggatcccgct gtcgcgttat cctgactttg atgagattta cgtcgaagga agctccacct
    49861 atcagttatt tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag accatcgata
    49921 tctttgccgc cacccgcccg caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt
    49981 ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc tccgaactct
    50041 tctgccgcgc caatcgcatc accttaacga ttgataataa aagacattaa ccgtaggccg
    50101 gataagatgc gccagcatcg catccggcga tgctggcgca ctgaatttta catcccgcac
    50161 gttcccctca ccctaaccct ctccccaaag gggcgagggg accgttcagt gcggaattta
    50221 gcttgattgc cgaacggtgc tatccagcat taaatactcg caatactctc acgcaaaatc
    50281 cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca atgttcgagt
    50341 tgtggattcg cctggcgaaa cgttcctgaa agtcgataga agagcggcgg gatttcatat
    50401 ttcgactctg caccgaccgg gtaaggcaac gcgccacgct tttgcgcttc ttccagcacc
    50461 ctggcggcaa tcagctgatg aaactcgaca atcaacacct tcgactgcgc attggcaatg
    50521 acggcgcttt tcacttccgc caccgcgccg ccgttaagca aagccagcaa ccgttcggat
    50581 actcccgcct gcaccgcatg catcactggc gcgaacacca gaccacgcaa gacttccagc
    50641 gcctgtgagc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat ccgactaata
    50701 acatcggcat cgccaaccac tgcaccaacg ccccccggcc caaacagctt gaagcaggag
    50761 aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ctaccttcat caccgcatag
    50821 ttatcatcgg ttaacgctgg aacacctgcc gcgcgcaacg ttgccagcac atctgccagc
    50881 acatagctgt cctgcggctg ctggcgcgta tgctgcacca gcgcggcatc aggttgttgc
    50941 tcgtcgacga cctgcttcag tgccgacagg tcattgaaat cagcagtaat aagcgtcagc
    51001 cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg caccagaaga
    51061 cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa tcgcgccagt ccccgcgccc
    51121 tgcaccagcg ccgcggcctg tgcgtgaaat gcatcagcca gcacctgctc cacccgttgg
    51181 gtaatacgcg gttgattcag acttggagtt aaccctaaat caccgccagc aagaaactcg
    51241 ctgcctggaa aatggcggca aatagtgtcc accagcgcaa actgcttttg ctgcgcttca
    51301 gcaagagtca ggctttgcaa aggaaacgtc ttcataggtg ccttccttaa gccggaacga
    51361 acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact gccacgaccg
    51421 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaaccg
    51481 cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa ccgaccagca
    51541 gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgcaccg
    51601 acgggtagcg tcccagccat ttgccgatcg aacgaagcag caagacttcc gccgaaataa
    51661 ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc accgcataaa
    51721 caaaggtaaa gcccgcaact gcatacacac ccgttgctaa ggcggtggtg gcaatcaacg
    51781 gcacaaaccc cagtccgcgc ataaattctg ccagagccgc ctgattaatc agcgtttgcg
    51841 attgttccgg cgtcacgcct gcggaatacg ctttctccag tgtgaagatc gaaacttcgc
    51901 taccagcaaa aatcttcata ctggcaacgg cggcaatcaa tgctcccacg atggcgatat
    51961 aaggtaagtt tttgataatc cgcgacgtgc gctcttcaaa caccgaaagc ccgctggcat
    52021 cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc agcagcatca
    52081 ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag cgcacgacta
    52141 ccacacgggt catcagaaca accacggcgg cgaccagact ttgcttccag ccaaactggt
    52201 agaaaatcgc caccagcggg aacagtgcaa aagcagaaac caccggcgag cttaattccc
    52261 cgaggctacc taatacatcc accggcagcg cggtcagcag ctggttaact ggcaacaggc
    52321 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
    52381 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc agcccggttt
    52441 tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca aaagcgatac
    52501 tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc gggtggatgc
    52561 cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc gtcaggcacg
    52621 ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca gatggttaat
    52681 caacatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca ctttcccttc
    52741 agcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg caatggtgca
    52801 acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg caccggtatt
    52861 acacgcgcca atgtaataat ctagttgccc ggatttcacc ttcattgccg cttccatgtc
    52921 gttgtgaatg aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg
    52981 ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat tggaaaaatt
    53041 gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcgca
    53101 attgcggaat aaaggtggtc aataaatagt catagccata accaccattg gcttttaaat
    53161 gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta
    53221 gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta ccaatggtgt
    53281 cgaactgcac gtacgcgccg agatcgatca tcttcaaaat gttgtcgagg ttgtctttca
    53341 gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca
    53401 gttgttccag ccccatggtg ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt
    53461 tatgcgccag cgcagcggca ataaatacct tctcttccag cggcgtaatc tttccttcgc
    53521 tggagccgat ctccgcgatg atcccggctt tcagctccgt gccatcgata ccctgttcaa
    53581 tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcaccaca tgttccggga
    53641 aaaacgcatc ctggtaataa ccagtacagg ccaccacgtt gatcccggtt tcgcgcatta
    53701 catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac
    53761 gtacaccccg ggtcatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc
    53821 ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca tgggccaggg
    53881 tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa
    53941 agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag ctcccacatc
    54001 agaaagcgtg gtgcgcacac cgagctgcgt ggcgaccagc ccttgctgat aaaccagcac
    54061 tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc
    54121 catcacgacc aggcaatcat ctggctgcat cgcctcaaca agccgggcaa ggttacggtc
    54181 aacgacctgc aaacgttcgg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt
    54241 ttcctggata ttgatgcaaa taaacgccgt cggatgggtg ttaaattcgt cgagggtgat
    54301 atccataatc cgctggctat ccaccaggtt ttgccagctc acaccataag gattgctgac
    54361 gatatctgcc accttaccca ccagcacggt tggcacgccc gcttcataca gtttttgcgg
    54421 cacctgcact ttttcatcaa caccgtagcc catatgcacg acctggaagc cgttgtcata
    54481 agcgccagaa cgcggcgcat taataccaat aaagcgccct tctttgcttt ctgcggcatc
    54541 gagaatacgc tgactgttgg ttaacaggcc gccaaatgta atgacccgac cgacctgtac
    54601 ctggtcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca cagagagatt
    54661 ggcggtaatg ttatagactt ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc
    54721 ctgattgacc gacagaaatt gcagatcatc gccacggcgc tccacctgcc agccagcgga
    54781 aactaatgcc tgctcaacac ggtcaatcac atcgcgaaaa ggcatccgca gcggcggtaa
    54841 cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag
    54901 ctctgccacg ccccaggttg cggaatctga cggctgcata tcgcctggcg cataacccaa
    54961 tgtgttgatt agccccagct tctccagcgt tggtagctgc aaatgcggca actggctcag
    55021 gatgtgacca caggtattcg ccccggcatc ttgcgggcgc accagcgtga catctttcat
    55081 tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccattt atgcctccag
    55141 agggttgccg aggctgtcat aacgaccaac tatttctggt tcgccgcgct gaataccgga
    55201 caccagcacc acatcgctac gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc
    55261 actgctcacc ggatactcgc ctgccagcgg cagggtgtag tcgatactgc tgtcatccac
    55321 agttttgaga ttggtttcag taatcttttg attttctggc gtaaacacca gcgcatgttg
    55381 cgcatgacca cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg
    55441 ggagatttca cttaaccaga gcatcgcgat acgttcaggc tgatcgccct gctggtttgc
    55501 cggaatagta ccggtcagcg catgaccggg ttcggcatga gtcgcaccgt attccgccag
    55561 caatggcagc gaagtgcagc tggtcgctga aggtacgttc agttgctcaa tcgcaatacc
    55621 agatttcgcc agttgatccc gtgcttgtac cagcgtgtga agattcggtg ttggcaaaac
    55681 tttcccagca gcctcatccc aaagcaggca agggaaatgg gttagtccgg ctaaatgcag
    55741 ccccggcagt ttcttaattt cagcaatcac ctcgtgcagc gaatgctgta caaaaccgct
    55801 ctcctgcccc ggataaagaa aatcatcgtc gctataaact ttgagcagca caggctggac
    55861 ccgtccggcc ttaaccgctg ccgcagaaac ttcccgcgct ttgtcgagag taaacacggt
    55921 gatgatgtcg gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag
    55981 atgcccctga tgcgccactg gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc
    56041 caccgccaca atgccgctgt agcctaatgc cagcaatttt tccgccagcc acggattacg
    56101 accaaattgc ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatgag
    56161 ccgcttaccg ttttccagta tctgatccac gtcgatcacc cagctgtccg gggcgatttt
    56221 gccctgctgc cacaaactta gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc
    56281 ttctacaaac atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttgaa
    56341 tatttaggtt gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc
    56401 cataatccat aaagattggc cagaaaatag ccttcttcac acgggttaac ttccagagcg
    56461 aattccttca acaactcctg atgcaattgc acaacggctt gccagtggct ggattgcgcc
    56521 agttccgcca gcaactcgtt atccagcggc tctatttctt caccacggcg actgcgcatc
    56581 agtgcactcg ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg
    56641 cactcttttt ccagtacgtt gacgacctgc atcatgcctt tgcagacgtc cttatcaata
    56701 acgcctgcct cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata
    56761 actgggttcc tgttccccac tcaccacccg ttgttgatgg gcaagcaggg cgtgtttatc
    56821 tacaacagca cgcagcaatt gttgcattgg gtaatcatcg actcgcgtca ggaccacggc
    56881 ttcggtggcc tgtaaaaatc gcggatcgtc tgttagcggc gtcgcctcta atcccagcat
    56941 ggtcagttcg ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat
    57001 gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc acatcactat cgccaaaaaa
    57061 gacatcggtc atgattttct gatccgccga acgactatcg agacctactc gcttaacatt
    57121 tccagactca cctttgcggc atatcagttg atgttcacca acatacgtat gcgggccaag
    57181 ctcgagggcg atgcacaaat tgttttggct gagataactt tccgccgcca ggcgggaaac
    57241 tactgccatg tcatagacac cgttaagtaa acactccacg cgaatatccg cgccgcgcat
    57301 atgcgcatag taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc
    57361 gtacaagcgg gtatagggca aaggcatcgc acataccacg ttgttgatat ccacatgact
    57421 cagcaatgct ttgttatcca tctcgacaag ataactgcca ttgcgcccac ggcgttcaat
    57481 ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caaccgatga
    57541 acggcattca gttgccagct catctatggt tttcagtcga ttaccgcatt tttcacccaa
    57601 caaataacgg gccagtgtcg tgatgacgac gccttctttt ttgataaacg ttcgacgcat
    57661 aatatatttt caataaaatg aatatttata tcttcattaa aatgaagata agtggcgacg
    57721 acgagatgtg gaaacggcga ggcgcttcac attttttcgg gttgttgcga aaagaggata
    57781 aaaaaaccgg gtttccccgg tttcagagtg atgacgaagt gatttattcg acaatgcgag
    57841 caaagggtaa ttcccactgg cgcttgcctg ctcccctcac cctaaccctc tccccaaagg
    57901 ggcgagggga ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg
    57961 tacaccgctt tcagcgtacg ggaagcgtgt acactggctt tttctgcgcc gtctttcatc
    58021 acctgttgca ggaaggcttc atcgttgcgg aaacggtgat agcgttcctg caattcagtc
    58081 agcataccgg aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg
    58141 aactgttttt ccagttctgg gatgctctgg cccgttaccg cagaaaggat atccagcagg
    58201 ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca
    58261 gtgaccgcgc gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg
    58321 ttattgcggt tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg
    58381 cgcgcgccag atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg
    58441 ttgaaacgct gggcgatatc gcgactcagt tccaggtgct gtttctggtc ttcacccacc
    58501 ggtaccagat tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagccaaac
    58561 agaccagcgt tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcga
    58621 ctcagttcgc cgaagtaggt atagcagttc agtgcccagc ctaactgcgc atgttctggt
    58681 acgtgggact gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag
    58741 gccagcgtat ccaacgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg
    58801 tgttggtcaa cgatacagta aacgcaatgg tagtcatcct gcatgtttac ccactgacgc
    58861 agcgcaccca tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg
    58921 atgggcttag tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag
    58981 aaggtcattt atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta
    59041 gttgtatccg taggttaagc caactgatgg gcaacctgcc gcttttgccg cctgaatatc
    59101 attccgtgag tcgccgacaa acagcatctg ttgtggggca attcccatcc gctcagccac
    59161 cagtaacagc gggtccggat gcggtttttt gttttgcaca tcatcgccgc cgataaccac
    59221 gctaaagtat ttggcaatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt
    59281 gttggtgacc aagcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac
    59341 gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt
    59401 acgcagaata cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttccccat
    59461 tgttttacgc agagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac
    59521 atctgcgccg ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gcaactccag
    59581 cgcatacagc gccatatcta ccgcagcagc aagaccagga gcactgtcga ccagcgtacc
    59641 atcaagatca aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca
    59701 gttcactgcg catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac
    59761 cggcgacgaa catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc
    59821 caccgtccac ttccagacga atgtcaaagc cagactcgtc gatacggcga cgtacttcgc
    59881 gcagtttatc cagtgtttga ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca
    59941 tcagcaggat cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg
    60001 ggttaaatac cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa
    60061 catgctcgga ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag
    60121 gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccgt
    60181 agttacgcaa ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat
    60241 ccatgacgtc aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac
    60301 ccaggcgggc aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct
    60361 tctccttgag aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag
    60421 ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac
    60481 atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag
    60541 cactggaata tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc
    60601 aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggtgcata
    60661 aggcggatcg caatagacga cggatgcatc atctgcgcgc tccatgctat cggcgtaaga
    60721 ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg
    60781 gaaatagggt tttttgtagc ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg
    60841 acacaggccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg
    60901 atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc
    60961 gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt
    61021 atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt
    61081 gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg
    61141 ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt
    61201 tttcttcatg ccgattaact aattacacct tctccggctg tggagaaagc tccgacagca
    61261 tccagcgctt caggcagctc ccgcaacgat aaaccattgc gggagataac cttgattact
    61321 tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg
    61381 ctggcaatgt agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca
    61441 ggacatacca cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct
    61501 ctttcttcgc ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt
    61561 aatggctgga cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg
    61621 ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct
    61681 cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg
    61741 gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg
    61801 gttttttcgg ttcaatcacc gcctgctgac gaactgggcg cgtagtggac ggacgttcag
    61861 cggtttgcgt tttcgccgag tcacgcgatg cattgccatt gcgaacaggc gcaaccgttg
    61921 cgggttcagt cggcaacgtg gaattgaccg ccacattgtt cagctgttgc tgattttgtg
    61981 gctgggtcag cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg
    62041 ccaccggggt ttgcccttga gtcggcgtag aagagatcgg tggcagcgaa acatcctgct
    62101 gagtattttc cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg
    62161 cattgccagc aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg
    62221 agggggcttt tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga
    62281 cgcccatcat catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg
    62341 gacgacgctc tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt
    62401 cgatttccgg ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt
    62461 catcaagttc aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac
    62521 gttcagaaga ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt
    62581 cttctggttt gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc
    62641 aattaacctg aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct
    62701 gattgacaat cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca
    62761 ctcttaccaa ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca
    62821 cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac
    62881 ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga
    62941 cgttccgacg tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa
    63001 ttgccatacc ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa
    63061 gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa
    63121 cggcgaatac agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct
    63181 tccagccagt taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc
    63241 cccgacgcta actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa
    63301 gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg
    63361 ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca
    63421 ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc
    63481 gccaccagcg tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg
    63541 agtacagcta ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca
    63601 cccgcctgtt caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgctg
    63661 gtgaccaaca tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac
    63721 aaaccagatg cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc
    63781 ataacgcgac atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt
    63841 agttgctttc cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag
    63901 tacgaatggt cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt
    63961 ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg
    64021 tgcgtgcaag ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa
    64081 gacggttacg cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct
    64141 gtttctcggt caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt
    64201 ctaaatcgaa aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat
    64261 cgtaaaattc catattgagc tgttgagcta actggcgccc aatagtgctt tttccggcac
    64321 ccataggccc aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac
    64381 tattcgttaa tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct
    64441 cataagatat tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac
    64501 tgaataaatc acccatccct ctgcatatct ggtcgctgca agcgcgctgc cttgctacca
    64561 ccgctctggc gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct
    64621 gaatgataaa tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta
    64681 ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac
    64741 attaccgctt ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact
    64801 aactcgcgtc gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg
    64861 tcgccaagca acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc
    64921 agcgccaacg tttctccgct ttttacctcg acctgcgttt cgatctcctg cttatcaatc
    64981 gccagcactt cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt
    65041 aatttcagcc ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg
    65101 gcctctttaa attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt
    65161 tcgctctcct gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg
    65221 ataatatcca gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg
    65281 ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta
    65341 ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc
    65401 acgcctaact cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc
    65461 tgcccgaccg gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg
    65521 ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc
    65581 agtagcttct cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg
    65641 cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata
    65701 ttgtcattct gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc
    65761 agtccggcgc ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt
    65821 aacgacaccg taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc
    65881 agcgcctgca acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc
    65941 tttgccgcct gtacgccggg tatcagcatc aacagtagtg cggctatcca ttgcttcatt
    66001 tgtatctcct tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggtacccag
    66061 cgttagtacg tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg
    66121 ctgcacccgt cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg
    66181 ccctaccatc ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg
    66241 cgatttaaaa gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca
    66301 gcgtttaacc ctcatgcggc atctccagtt gtagcgtgaa caaaagatca tcaccttcca
    66361 cgcttaacga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    66421 gcactgcttc ccacagcgtt ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg
    66481 aaaccagttg cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct
    66541 cgctgaaggg agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg
    66601 cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg
    66661 taactaacat cagcaaccag aatgcccagc aaaactggcg gaggcggggt gatgtggcga
    66721 accaccagtc aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc
    66781 cagcgtccct gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc
    66841 tggcggagtg acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc
    66901 tccagcgttc cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc
    66961 gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc
    67021 gaacgctgtt cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg
    67081 cgggcgagtt gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct
    67141 gtcagacgca gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc
    67201 aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    67261 gggttcatca gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccaccag
    67321 gcggcggtag cggcggctga cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc
    67381 cacatatcgc gacgctgtcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    67441 tcatccccac cgccagtttg cgcccccagc tatagcgcgt cgcccacagc cactgttcgt
    67501 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    67561 cactggcatc cggggtgatc gcactaacat gaacacgcaa cctttctgcc agcgtaagca
    67621 gcgttgccag ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt
    67681 cgctgtaatc gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg
    67741 acagccaggc cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct
    67801 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    67861 tccacggtaa caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa
    67921 tgtcgtgcgc cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca
    67981 cgatcgcaac cgctgccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa
    68041 atgtcattgt gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg
    68101 cttgccttta tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa
    68161 tttccagtga agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga
    68221 gcaggctcga tttatggcct ataccgctac atcgagccac aactgccgga tgtggtgaca
    68281 ttaaaagatg ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt
    68341 gctcaatacg gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg
    68401 gtgaaagcct ttatcgcgac ggaagacagc cgcttctacg agcatcacgg agttgacccg
    68461 gtggggatct tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg
    68521 agtaccatta cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgatgatg
    68581 cgtaagatta aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa
    68641 atcctcgagc tttatctgaa caagatttac cttggttacc gcgcctatgg tgtcggtgct
    68701 gcggcacaag tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg
    68761 atagccgggt tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc
    68821 gtcgcgcggc gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccagcaa
    68881 cagttcgatc agacacgcac tgaggcgatt aacgctaatt atcacgcgcc ggagattgct
    68941 ttctctgcgc cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa
    69001 agtgcctatg aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc
    69061 gcgcagcagg cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc
    69121 ccggcaaatg tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat
    69181 acgctgaagg cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat
    69241 cctcagggag cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc
    69301 gttcgctggg cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg
    69361 accgatgttc tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg
    69421 ctggcacaag tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc
    69481 gttatggcgc tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag
    69541 gcactgcgtc aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa
    69601 ggtctgacgc tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc
    69661 ggttctgact ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt
    69721 caggggctgg gtcagtcgaa aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc
    69781 gactacgctg cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc
    69841 gaatcgctgg cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg
    69901 gtcatggcga acggcggctt cctagtggac ccgtggttta tcagcaaaat tgaaaacgat
    69961 cagggcggcg tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattcca
    70021 gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc
    70081 gctatctccc gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat
    70141 caggcgttag tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg
    70201 ctggcattcc tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag
    70261 ggtaccggct ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt
    70321 accactaaca gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg
    70381 gtctggattg gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg
    70441 attaaagatc agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac
    70501 gcttatatga aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt
    70561 attgtgacgg tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc
    70621 gaagagtatt tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg
    70681 accattatcg ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc
    70741 ttttcagttg gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg
    70801 gctaacaaaa tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg
    70861 tgcatcaggc aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg
    70921 aggaacagcg cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc
    70981 atatgcgcca gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat
    71041 tccgggtaga gatcttgcgc gaccacgata ttcattttgc tggaaaagta agacggtgcc
    71101 atgctgagct tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc
    71161 tcgcggttag cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct
    71221 aattcgtagg attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcatccaca
    71281 atcggcacaa tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc
    71341 acgccattgc tgaactccag atccacgctc tcgacggtaa acagtcggga acgggctaca
    71401 gtttcaacat tcagaatggt gggtttttgt aatgatttgt tcatcgtggg atctatgctg
    71461 tgaaatcagc ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta
    71521 tttacattta tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt
    71581 gaaataactc caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt
    71641 tactgtgatg tgcttaatga tgctcaagtt aagctccacg cttgccgata gccaaccgca
    71701 gaatcatgta ttgtgtccgg tgcgactgac cacgcctgac agactaagta agatggggaa
    71761 agcatgagca ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga
    71821 tggctgataa aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat
    71881 gcgcaaacgc gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc
    71941 ctgacgcagg tattacaggt gcctggccca acgggagcta gcgcggcacc gatctctctg
    72001 gcgctgaatg ccgaaagcaa caacgtcatg atgctgacac acgctatcac acgttacggc
    72061 atctctaccg acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg
    72121 ccccccttct gggaacagta catcaacgat gagaataccg ttgaactgat tcataccgat
    72181 tcgctgccgt tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg
    72241 cgcggctatg ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt
    72301 gagcaaatcg agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt
    72361 ccgcgcgggc tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc
    72421 ctgattaccc cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc
    72481 gcaggtctgt gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt
    72541 ctgcgcggta caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac
    72601 aatatttcac tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct
    72661 caggatctcg gtcaacaaac cgatattgat atctatctcg accgccacgt agtgcgtcag
    72721 gggcgatatc tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc
    72781 agtacgatta tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctggatcccg
    72841 ctggatatgc cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc
    72901 accagcgtaa aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc
    72961 ggtacgggaa tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct
    73021 tttttaccgt ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca
    73081 gaatctgaac tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac
    73141 cctaaaccgg aagatgaatc tcgcgtcagt gcctcattgc gttcagcgat tcaaaaatcc
    73201 ggcatggtat tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct
    73261 gccaaagatg actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac
    73321 tgggacgcgc tggtaaaacg cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta
    73381 cgcccggtga gtgcggaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc
    73441 acgcatgaaa cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg
    73501 attgtcagcg acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac
    73561 gactacccgc cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat
    73621 acgccgttta acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag
    73681 catattggcc ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca
    73741 ttgctgctac tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt
    73801 taccagcgtc atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac
    73861 ccgcaactga tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat
    73921 gctaccctgt cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc
    73981 ctggcaggac gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt
    74041 cgataactat ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac
    74101 gccacaggaa gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa
    74161 ctggtactgt cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac
    74221 cgagatggga ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa
    74281 agccagcggt aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa
    74341 acttgagcat acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg
    74401 ttatccgaaa gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc
    74461 tgaaagaacg ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg
    74521 tattcgttac tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca
    74581 acgccatccg tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg
    74641 ccatgaaaga gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt
    74701 tttataaaac ccgcgcgctg gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg
    74761 ggcagcgcag caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg
    74821 gaaatgacga acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg
    74881 aggcagcctt gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga
    74941 tggcacgtaa acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag
    75001 agcgccgcga cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag
    75061 agatgattat gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc
    75121 gcggcgaact ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc
    75181 cgcagcccgt taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta
    75241 cgctgaagtt tgatggtgat atcaccgtac agctgcaggg cgacggtccg atgaatctgg
    75301 cggttattaa cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa
    75361 ttccagaaaa tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta
    75421 ccccgagcga aggcgaacgc tatcagggcg tagttggtct ggaaggtgat accctggcgg
    75481 cctgcctaga agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca
    75541 ccggcgacgt agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc
    75601 aaaataccca acaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg
    75661 aagaactgct gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg
    75721 tgacggttta cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg
    75781 ccgatgcgct gaaaacgctg cctgatgaag aagttgatag catcctggcg gaagatggcg
    75841 aaattgacat gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg
    75901 ctgaaatccg caacaacgcg tctccggcag atccgtaagt tcattaatga gtatgtccgg
    75961 cagagagggc tctgccggat tttacgactt aatggcctga tgtgctgcgc ttaccagcgc
    76021 ctatgaaata aatcgcaaat tattttggtc agatacagcg tcgtatccga taaaagcgtt
    76081 gtcacttact taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt
    76141 tcggcctcgt ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt
    76201 cgttcggaag taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat
    76261 agacaatgac tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat
    76321 tcagatcttg ctcgaccagt acgcaggcgt ctttttcaat acaggtcatc acgttatagc
    76381 gatcctggat taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta
    76441 acacattttc ggcgaatact ttttccgata cctgttcctg aagatgttgc tctccatcta
    76501 acgccatcag cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca
    76561 gcgactcaag cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct
    76621 ggtctggcgt gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca
    76681 tactgtccag caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg
    76741 caaggttcac tttgccctga agaacaaggg gattttgccc attacgccag acgatactga
    76801 caaaataccc cagcgaccac accagtaaca ccagctccgc cagcgcgccc tgcaagcggt
    76861 cgacagtcca gccgtactga gcgacccgca gccataaccc ccaggtggcg acgaacacat
    76921 acagcggagc aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa
    76981 gtgacgcttt ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg
    77041 ttaacagcaa cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca
    77101 gggtgatgat aaacatcggg gttagcaatg atactaacgg cagcaacccc gtggcgatta
    77161 gcgtgaacaa cttttgaata gagtcgatta aacgtgactg tgtacgcgcg aggatcaccg
    77221 ccagtgcggt aaccagacca aatgtaagat acataaacca gtcggttgca aaaaagagcg
    77281 tattgaaaaa cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc
    77341 acgtcaggcc attggagaga aaaatcacca gtaatataag tacgttatgc catactgact
    77401 ggtaaaagta gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg
    77461 gtagcaacaa cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag
    77521 ctctccaggg gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg
    77581 caataaacac cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga
    77641 agagtaaaac ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgtgat
    77701 tttttccggc aagccaggcg ataagcaggt agcaaaccag cccctgaagt aatccggtag
    77761 caatcatccc ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga
    77821 atcaaaaggt gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata
    77881 atcaaaaaaa gttagcgtgg tgaatcgata ctttaccggt tgaatttgca tcaatttcat
    77941 tcaggaatgc gattccactc acaatattcc cgccatataa accaagattt aaccttttga
    78001 gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga
    78061 atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc
    78121 gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag
    78181 tgaaatgcgc gttaacaatg gtttgccccc gcaagaactc gaggcttatg gtatcagtga
    78241 cgtacatgat atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc
    78301 gagcctgaca ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac
    78361 cgggatcttc accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg
    78421 cgatactttc tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc
    78481 ggaaacctgg cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt
    78541 cgttgtcgac gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac
    78601 cgaagtggcc tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga
    78661 gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca
    78721 gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat
    78781 gcagctgatt ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat
    78841 gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga
    78901 gaaaggcgat gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac
    78961 cgacccgaaa cgccgcctga ttggcgatga cgaacacggc tgggacgatg acggcgtgtt
    79021 taatttcgaa ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga
    79081 aatctacaac gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg
    79141 cactatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccaatcta
    79201 tcacatcgat aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt
    79261 cctgactgct gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac
    79321 ccagtatcac ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac
    79381 cgaaccgacg ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac
    79441 tcagtacgca gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt
    79501 taacactggc tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat
    79561 cgacgccatc ctcaacggtt cgctggataa tgcagaaacc ttcactctgc cgatgtttaa
    79621 cctggcgatc ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac
    79681 ctacgcttct ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga
    79741 caacttcgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa
    79801 actgtaatga tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga
    79861 ggcaccttcg cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg
    79921 gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca
    79981 tcccgttatg gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa
    80041 tggtgcgcgc actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg
    80101 caattcccgg accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc
    80161 tgactttgat ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc
    80221 gtttgatcga cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa
    80281 tttcccgctc atagccactt tcggcagcaa tcacctcacc gagcactgca ttaagatccg
    80341 ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg
    80401 cgttgcactc ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca
    80461 tcatctcagt cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg
    80521 ccatcagcag cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg
    80581 cacgggtaac ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc
    80641 ctttaccaac ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa
    80701 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    80761 gagagaaatc gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca
    80821 gccaggtttt cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    80881 ggccgcccag ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca
    80941 gacctgcctc ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct
    81001 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    81061 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    81121 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga
    81181 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    81241 agagccgtcc ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg
    81301 cgctggatct tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    81361 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    81421 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
    81481 ctcatcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    81541 taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc
    81601 acgaatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
    81661 aatctccagg cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
    81721 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    81781 cattaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    81841 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    81901 acgcaggcgc atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac
    81961 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    82021 agctaaatct ggtataaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    82081 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    82141 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    82201 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    82261 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    82321 atcagtataa taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    82381 gcctggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa gtattttttg
    82441 gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct
    82501 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    82561 tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgaact
    82621 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    82681 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    82741 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    82801 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    82861 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    82921 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca
    82981 tttccgagca aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca
    83041 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa
    83101 tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca
    83161 cgccagaagt cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag
    83221 ccgcgctgga tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc
    83281 tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg
    83341 gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca
    83401 cggtgccttc tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc
    83461 tttcgctgaa tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa
    83521 tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaaa
    83581 gcgtagtgag ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg
    83641 gtaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    83701 atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc
    83761 gtaccggggt aaaagtggcg gtggtcgatg ccactggcaa actggtggcg accgatacca
    83821 tttacccgca caccggacaa gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa
    83881 aacataacgt tgaactggtg gcgatcggca acggtacagc ttcccgcgaa actgaacgtt
    83941 tctatctcga cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg
    84001 aagctggcgc gtcggtttac tcagcttccg agctggcagc gcaggagttc ccggatctcg
    84061 acgtttcgct gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc
    84121 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    84181 cgcaactggc ccgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    84241 atctcaacac cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg
    84301 cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgc
    84361 tgaaagtcag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta
    84421 accacggtga taacccgctg gacgcctcta ccgttcatcc ggaagcctat ccggtggtgg
    84481 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    84541 tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg
    84601 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    84661 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg
    84721 cagtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc
    84781 tggttcacat ctcttcatta tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag
    84841 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    84901 tgactatgcg tctggacgag cagcctggcg aaaccaactc tcgtcgcggc ggcggtaatg
    84961 aacgcccgca aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg
    85021 gtaatagcgc gatgatggat gcgctggcgg cagcgatggg taaaaaacgt taaacgcccg
    85081 gactggcccc taaacctgcg cgataaggcg ttcacgccgc atctggcaac gaacgctttg
    85141 cctgacataa aagtgccgga gaatatctcc ggcactttta ttccacagcc aaactcacaa
    85201 tatactccgt taatatttat cacttcatta acaactgaaa ccttaattaa acattagcca
    85261 gtccgggtaa ctcactattc gaattatatt ttcgctgcga tataaccttg agccacatca
    85321 acattgagtc agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag
    85381 aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca
    85441 tctggtctca tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa
    85501 gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga
    85561 aatcagcccg gcatatcgcc aaaaactgct ttctctaggc atgttacctg gctcctcttt
    85621 tgatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag
    85681 cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata
    85741 cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca aattctggca
    85801 agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg
    85861 ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg
    85921 acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa
    85981 tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt
    86041 ctaaccttga gcgtaacctg tacctgacgc tgcaactgct ggaactcggc attccctgca
    86101 tcgtggcgct gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg
    86161 ctctatcggc acgtcttggc tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta
    86221 ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc
    86281 attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca
    86341 tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc
    86401 gcgcctacgc cggtgaagcg tcgcagcatc tggatgccac cctcgcccgt ctgcgtaatg
    86461 aaatggatga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct
    86521 gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata
    86581 aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt
    86641 tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttaacgtc ggctccgtgg
    86701 cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga
    86761 ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc
    86821 agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat atggcgcgtg
    86881 cagcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc
    86941 cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg
    87001 caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc
    87061 tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcc ctggcggttt
    87121 tctcgctgta tatgctcggt atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca
    87181 ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac catgtaccac
    87241 acgttaaaag cctgattatc cagacctggc agcgtctgaa gggcttcgtt ctgcgtgctg
    87301 gtaaagtgat tatcatcgtc agcattttcc tgagcgcttt caacagcttc tcgttgagcg
    87361 ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgataaccc
    87421 ctgtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta
    87481 cgggcgcaat ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa
    87541 atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg
    87601 cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc
    87661 ccattgaagc tagcaaaggc aacggtgaaa tgggtaccgg ggcgatgggc gtgatggatc
    87721 agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac
    87781 catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct
    87841 ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca
    87901 gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggtaatc ctgtttaaca
    87961 tcgtagctat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa
    88021 cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc
    88081 ttcacttatt caggtacgtg atttgctggc gttacggggc cgtatggaag cgacccagat
    88141 aagccagaca ttgaacactc cgcagccaat gattaacgcc atgctgcaac agctggaaag
    88201 catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa
    88261 aagctgcccg gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc
    88321 catcgcctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt
    88381 tccataaaaa tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt ctcgcaggga
    88441 tgaaaactcg ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca
    88501 ggtgaaggaa cgccatgagt aaaaagcaga gttccacccc acatgatgcg ctgttcaaac
    88561 tctttttacg ccaaccggag acggcacgcg attttcttgc gtttcattta ccggcaccca
    88621 ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gaggagcttt attgatgacg
    88681 atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat
    88741 acatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga
    88801 tgcgttacgc tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga
    88861 tggtggtgcc attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt
    88921 ggctggattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc
    88981 tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc
    89041 tggagttaat tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg
    89101 cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca
    89161 tactgcaaac tggcgacgct gtacgtttta acgattttat cgacggcgtt gccgaacgtt
    89221 caccgaaaca caaggagagt ttaatgacta ttgcggaaag attgcggcag gagggggaac
    89281 aatccaaagc cctgcatata gccaaaataa tgcttgaatc cggagtccct cttgcagaca
    89341 tcatgcgatt taccgggctg tcagaagaag agttggctgc ggcgagtaga ctggccccct
    89401 gaatctccag acaaccagta t
//
LOCUS       DAEBXD010000002        78106 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEBXD010000002 DAEBXD010000000
VERSION     DAEBXD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78106
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.002"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..586
                     /locus_tag="KJL94_000105"
                     /pseudo
     CDS             1..586
                     /locus_tag="KJL94_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            689..1012
                     /locus_tag="KJL94_000106"
     CDS             689..1012
                     /locus_tag="KJL94_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBD4408628.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1079..2536)
                     /gene="nuoN"
                     /locus_tag="KJL94_000107"
     CDS             complement(1079..2536)
                     /gene="nuoN"
                     /locus_tag="KJL94_000107"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBD4408629.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2543..4072)
                     /gene="nuoM"
                     /locus_tag="KJL94_000108"
     CDS             complement(2543..4072)
                     /gene="nuoM"
                     /locus_tag="KJL94_000108"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBD4408630.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4236..6077)
                     /gene="nuoL"
                     /locus_tag="KJL94_000109"
     CDS             complement(4236..6077)
                     /gene="nuoL"
                     /locus_tag="KJL94_000109"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBD4408631.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6074..6376)
                     /gene="nuoK"
                     /locus_tag="KJL94_000110"
     CDS             complement(6074..6376)
                     /gene="nuoK"
                     /locus_tag="KJL94_000110"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBD4408632.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6373..6927)
                     /gene="nuoJ"
                     /locus_tag="KJL94_000111"
     CDS             complement(6373..6927)
                     /gene="nuoJ"
                     /locus_tag="KJL94_000111"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBD4408633.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6939..7481)
                     /gene="nuoI"
                     /locus_tag="KJL94_000112"
     CDS             complement(6939..7481)
                     /gene="nuoI"
                     /locus_tag="KJL94_000112"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBD4408634.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7496..8473)
                     /gene="nuoH"
                     /locus_tag="KJL94_000113"
     CDS             complement(7496..8473)
                     /gene="nuoH"
                     /locus_tag="KJL94_000113"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBD4408635.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8470..11196)
                     /gene="nuoG"
                     /locus_tag="KJL94_000114"
     CDS             complement(8470..11196)
                     /gene="nuoG"
                     /locus_tag="KJL94_000114"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBD4408636.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11249..12586)
                     /gene="nuoF"
                     /locus_tag="KJL94_000115"
     CDS             complement(11249..12586)
                     /gene="nuoF"
                     /locus_tag="KJL94_000115"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBD4408637.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12583..13083)
                     /gene="nuoE"
                     /locus_tag="KJL94_000116"
     CDS             complement(12583..13083)
                     /gene="nuoE"
                     /locus_tag="KJL94_000116"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBD4408638.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13086..14888)
                     /gene="nuoC"
                     /locus_tag="KJL94_000117"
     CDS             complement(13086..14888)
                     /gene="nuoC"
                     /locus_tag="KJL94_000117"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBD4408639.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14982..15644)
                     /gene="nuoB"
                     /locus_tag="KJL94_000118"
     CDS             complement(14982..15644)
                     /gene="nuoB"
                     /locus_tag="KJL94_000118"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBD4408640.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15660..16103)
                     /gene="nuoA"
                     /locus_tag="KJL94_000119"
     CDS             complement(15660..16103)
                     /gene="nuoA"
                     /locus_tag="KJL94_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBD4408641.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16734..17672)
                     /gene="lrhA"
                     /locus_tag="KJL94_000120"
     CDS             complement(16734..17672)
                     /gene="lrhA"
                     /locus_tag="KJL94_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBD4408642.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18592..19809
                     /gene="alaA"
                     /locus_tag="KJL94_000121"
     CDS             18592..19809
                     /gene="alaA"
                     /locus_tag="KJL94_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBD4408643.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19893..20492
                     /gene="yfbR"
                     /locus_tag="KJL94_000122"
     CDS             19893..20492
                     /gene="yfbR"
                     /locus_tag="KJL94_000122"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBD4408644.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20551..22383)
                     /locus_tag="KJL94_000123"
     CDS             complement(20551..22383)
                     /locus_tag="KJL94_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBD4408645.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22470..23120)
                     /gene="hxpA"
                     /locus_tag="KJL94_000124"
     CDS             complement(22470..23120)
                     /gene="hxpA"
                     /locus_tag="KJL94_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBD4408646.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23131..23625)
                     /locus_tag="KJL94_000125"
     CDS             complement(23131..23625)
                     /locus_tag="KJL94_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBD4408647.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23708..24163)
                     /locus_tag="KJL94_000126"
     CDS             complement(23708..24163)
                     /locus_tag="KJL94_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBD4408648.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24501..25703
                     /gene="ackA"
                     /locus_tag="KJL94_000127"
     CDS             24501..25703
                     /gene="ackA"
                     /locus_tag="KJL94_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBD4408649.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25778..27922
                     /gene="pta"
                     /locus_tag="KJL94_000128"
     CDS             25778..27922
                     /gene="pta"
                     /locus_tag="KJL94_000128"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBD4408650.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28112..29632
                     /gene="yfcC"
                     /locus_tag="KJL94_000129"
     CDS             28112..29632
                     /gene="yfcC"
                     /locus_tag="KJL94_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBD4408651.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29665..30207)
                     /gene="yfcD"
                     /locus_tag="KJL94_000130"
     CDS             complement(29665..30207)
                     /gene="yfcD"
                     /locus_tag="KJL94_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBD4408652.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30265..30816)
                     /gene="yfcE"
                     /locus_tag="KJL94_000131"
     CDS             complement(30265..30816)
                     /gene="yfcE"
                     /locus_tag="KJL94_000131"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBD4408653.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30872..31516)
                     /gene="yfcF"
                     /locus_tag="KJL94_000132"
     CDS             complement(30872..31516)
                     /gene="yfcF"
                     /locus_tag="KJL94_000132"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBD4408654.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31652..32299
                     /gene="yfcG"
                     /locus_tag="KJL94_000133"
     CDS             31652..32299
                     /gene="yfcG"
                     /locus_tag="KJL94_000133"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBD4408655.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32356..32718
                     /gene="folX"
                     /locus_tag="KJL94_000134"
     CDS             32356..32718
                     /gene="folX"
                     /locus_tag="KJL94_000134"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBD4408656.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32739..33632
                     /locus_tag="KJL94_000135"
     CDS             32739..33632
                     /locus_tag="KJL94_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBD4408657.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33680..34570)
                     /gene="rpnB"
                     /locus_tag="KJL94_000136"
     CDS             complement(33680..34570)
                     /gene="rpnB"
                     /locus_tag="KJL94_000136"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBD4408658.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34767..35540)
                     /gene="hisP"
                     /locus_tag="KJL94_000137"
     CDS             complement(34767..35540)
                     /gene="hisP"
                     /locus_tag="KJL94_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBD4408659.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35548..36264)
                     /locus_tag="KJL94_000138"
     CDS             complement(35548..36264)
                     /locus_tag="KJL94_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4408660.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36261..36947)
                     /gene="hisQ"
                     /locus_tag="KJL94_000139"
     CDS             complement(36261..36947)
                     /gene="hisQ"
                     /locus_tag="KJL94_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBD4408661.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37037..37819)
                     /gene="hisJ"
                     /locus_tag="KJL94_000140"
     CDS             complement(37037..37819)
                     /gene="hisJ"
                     /locus_tag="KJL94_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBD4408662.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38040..38822)
                     /gene="argT"
                     /locus_tag="KJL94_000141"
     CDS             complement(38040..38822)
                     /gene="argT"
                     /locus_tag="KJL94_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBD4408663.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39088..39657)
                     /gene="ubiX"
                     /locus_tag="KJL94_000142"
     CDS             complement(39088..39657)
                     /gene="ubiX"
                     /locus_tag="KJL94_000142"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBD4408664.1"
                     /translation="MKRLIVGISGASGAIYGVRLLRVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39752..41269)
                     /gene="purF"
                     /locus_tag="KJL94_000143"
     CDS             complement(39752..41269)
                     /gene="purF"
                     /locus_tag="KJL94_000143"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBD4408665.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41306..41794)
                     /gene="cvpA"
                     /locus_tag="KJL94_000144"
     CDS             complement(41306..41794)
                     /gene="cvpA"
                     /locus_tag="KJL94_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBD4408666.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42053..42715)
                     /gene="dedD"
                     /locus_tag="KJL94_000145"
     CDS             complement(42053..42715)
                     /gene="dedD"
                     /locus_tag="KJL94_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBD4408667.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42705..43973)
                     /gene="folC"
                     /locus_tag="KJL94_000146"
     CDS             complement(42705..43973)
                     /gene="folC"
                     /locus_tag="KJL94_000146"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBD4408668.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44043..44957)
                     /gene="accD"
                     /locus_tag="KJL94_000147"
     CDS             complement(44043..44957)
                     /gene="accD"
                     /locus_tag="KJL94_000147"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBD4408669.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45113..45772)
                     /locus_tag="KJL94_000148"
     CDS             complement(45113..45772)
                     /locus_tag="KJL94_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4408670.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45855..46667)
                     /gene="truA"
                     /locus_tag="KJL94_000149"
     CDS             complement(45855..46667)
                     /gene="truA"
                     /locus_tag="KJL94_000149"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBD4408671.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46667..47680)
                     /locus_tag="KJL94_000150"
     CDS             complement(46667..47680)
                     /locus_tag="KJL94_000150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD4408672.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47746..48882)
                     /gene="pdxB"
                     /locus_tag="KJL94_000151"
     CDS             complement(47746..48882)
                     /gene="pdxB"
                     /locus_tag="KJL94_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBD4408673.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48981..49976
                     /gene="flk"
                     /locus_tag="KJL94_000152"
     CDS             48981..49976
                     /gene="flk"
                     /locus_tag="KJL94_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBD4408674.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49973..51151)
                     /locus_tag="KJL94_000153"
     CDS             complement(49973..51151)
                     /locus_tag="KJL94_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4408675.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51406..52647)
                     /gene="fabB"
                     /locus_tag="KJL94_000154"
     CDS             complement(51406..52647)
                     /gene="fabB"
                     /locus_tag="KJL94_000154"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBD4408676.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52806..54812
                     /gene="mnmC"
                     /locus_tag="KJL94_000155"
     CDS             52806..54812
                     /gene="mnmC"
                     /locus_tag="KJL94_000155"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBD4408677.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54933..55211)
                     /locus_tag="KJL94_000156"
     CDS             complement(54933..55211)
                     /locus_tag="KJL94_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBD4408678.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55245..55793)
                     /gene="epmC"
                     /locus_tag="KJL94_000157"
     CDS             complement(55245..55793)
                     /gene="epmC"
                     /locus_tag="KJL94_000157"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBD4408679.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55793..56602)
                     /locus_tag="KJL94_000158"
     CDS             complement(55793..56602)
                     /locus_tag="KJL94_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBD4408680.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56602..57426)
                     /gene="mepA"
                     /locus_tag="KJL94_000159"
     CDS             complement(56602..57426)
                     /gene="mepA"
                     /locus_tag="KJL94_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBD4408681.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57430..58515)
                     /gene="aroC"
                     /locus_tag="KJL94_000160"
     CDS             complement(57430..58515)
                     /gene="aroC"
                     /locus_tag="KJL94_000160"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBD4408682.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58550..59482)
                     /gene="prmB"
                     /locus_tag="KJL94_000161"
     CDS             complement(58550..59482)
                     /gene="prmB"
                     /locus_tag="KJL94_000161"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD4408683.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59648..60199
                     /gene="smrB"
                     /locus_tag="KJL94_000162"
     CDS             59648..60199
                     /gene="smrB"
                     /locus_tag="KJL94_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBD4408684.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60303..61193)
                     /locus_tag="KJL94_000163"
     CDS             complement(60303..61193)
                     /locus_tag="KJL94_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBD4408685.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61180..61704)
                     /locus_tag="KJL94_000164"
     CDS             complement(61180..61704)
                     /locus_tag="KJL94_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4408686.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61701..62171)
                     /locus_tag="KJL94_000165"
     CDS             complement(61701..62171)
                     /locus_tag="KJL94_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4408687.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62168..62716)
                     /locus_tag="KJL94_000166"
     CDS             complement(62168..62716)
                     /locus_tag="KJL94_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4408688.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62721..63443)
                     /locus_tag="KJL94_000167"
                     /pseudo
     CDS             complement(62721..63443)
                     /locus_tag="KJL94_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63463..64185)
                     /locus_tag="KJL94_000168"
     CDS             complement(63463..64185)
                     /locus_tag="KJL94_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD4408689.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64245..65477)
                     /locus_tag="KJL94_000169"
     CDS             complement(64245..65477)
                     /locus_tag="KJL94_000169"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD4408690.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(65526..66104)
                     /locus_tag="KJL94_000170"
                     /pseudo
     CDS             complement(65526..66104)
                     /locus_tag="KJL94_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66186..66749)
                     /locus_tag="KJL94_000171"
     CDS             complement(66186..66749)
                     /locus_tag="KJL94_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4408691.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67433..67918)
                     /gene="sixA"
                     /locus_tag="KJL94_000172"
     CDS             complement(67433..67918)
                     /gene="sixA"
                     /locus_tag="KJL94_000172"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBD4408692.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68121..70265)
                     /gene="fadJ"
                     /locus_tag="KJL94_000173"
                     /pseudo
     CDS             complement(68121..70265)
                     /gene="fadJ"
                     /locus_tag="KJL94_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70265..71575)
                     /gene="fadI"
                     /locus_tag="KJL94_000174"
     CDS             complement(70265..71575)
                     /gene="fadI"
                     /locus_tag="KJL94_000174"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBD4408693.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(71755..72039)
                     /locus_tag="KJL94_000175"
     CDS             complement(71755..72039)
                     /locus_tag="KJL94_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBD4408694.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72411..73751
                     /gene="fadL"
                     /locus_tag="KJL94_000176"
     CDS             72411..73751
                     /gene="fadL"
                     /locus_tag="KJL94_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBD4408695.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            74502..75176
                     /locus_tag="KJL94_000177"
     CDS             74502..75176
                     /locus_tag="KJL94_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408696.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(75358..76113)
                     /gene="mlaA"
                     /locus_tag="KJL94_000178"
     CDS             complement(75358..76113)
                     /gene="mlaA"
                     /locus_tag="KJL94_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBD4408697.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            76407..77339
                     /locus_tag="KJL94_000179"
     CDS             76407..77339
                     /locus_tag="KJL94_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBD4408698.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            77413..77487
                     /locus_tag="KJL94_000180"
     tRNA            77413..77487
                     /locus_tag="KJL94_000180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77446..77448,aa:Arg,seq:cct)
     gene            77649..>78106
                     /gene="intS"
                     /locus_tag="KJL94_000181"
     CDS             77649..>78106
                     /gene="intS"
                     /locus_tag="KJL94_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBD4408699.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19731 a        20905 c        19232 g        18238 t
ORIGIN      
        1 ttttaccaac cctgggtcac tgcattatcg ccaacgcccc gacacgtaag tcctgagcaa
       61 ttagaaaaaa cagttcgtta tcttacacaa actgtgcatc cacgtaatgc cggcaatatt
      121 gataatctga ataggtccgc agagtatata aaagagttct ttgtcagtag cggtgccaga
      181 gttaccgcgc agggcgtccc cgattggcta ttacgactct gcgcctggaa gccagaatta
      241 cccttacccg gcaatgtcct ggctttatcc cgatcgggga gactttattg ctgtggtcgg
      301 cagaatgcag gatatcaacg ccgttcgtca ggtaaaagcg gcattgttgt catcccggga
      361 tttatctgtt tattctataa atgccccagg gtttattccc ggtattgatt tttctgatca
      421 cctcaattat tggcaacacg atattcccgc cgtaatgatt actgacaccg ctttttatcg
      481 taataaacaa taccacttgc ccggtgatac cgcagacaga ttgaattatc agaaaatggc
      541 tcaggtggta gatggtgtta taaccttgtt atacaacagt aaataataaa aatttctcga
      601 ggcgggtcca gaaacagcga catgtacatt ttattttccc tcgtctgttt taagatgcga
      661 aacataaaat tcaatggagg aaaaataaat gggaatgatt ggctattttg cagaaattga
      721 ctcagaaaag attaaccaac tgcttgagtc tactgaaaaa cctttgatgg ataatataca
      781 tgacacgctt tcaggattac gccgactgga atcactggat cgaagtgagc tgcgtaaaca
      841 gttttccatt aagcgactca atgaaatgga gatttatccg ggagtgacct tttcagaaga
      901 gttagagggg caactctttg cctcgatcat gcttgatatg gaaaagctga ttagcgcgta
      961 tcgacgtatg ctgcgacaag ggaatcatgc gctgacggtt attgttgggt gatggtgtca
     1021 gagcgccaaa aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa ctaacagttt
     1081 acatcagcgg cattgctaac cgcacaatac taatcagcgg ttgtggccat acacccagca
     1141 ccagtaccaa cagtgcagaa atcagcacca cgataccgcc cgcgctgtac tgccagtttg
     1201 acggtgcatc gcgacccggt tgttccggtg cgtgcaggta caggctcacc gccacgcgca
     1261 ggtagtagta gaggccgatt gccgaaccga caaccacggc acccaccagc caccacaagt
     1321 gtgcctggac accgaccgcc agcacgtaga acttaccgat aaagcccagc gtcatcggga
     1381 taccggccag agacagcatc atcaccgtca tcactgccgc gaggatcgga cgatgccaga
     1441 acagaccgcg gtaagagaac agggaatcag catccgggcc acgatacggg ctggacatca
     1501 ggctgaccac gccgaacgcg ccgaggctgc tgaacagata accggccagg taaaccccta
     1561 ccgcttccat cgacatctcg ccggtttgca gcgcaatcag cgccaccagc agatagccga
     1621 ggtgagagat agatgagtaa ccgagcagac gtttgatgtt ggtctggctc aacgccatca
     1681 ggttaccgaa gatgatggag gcaaaggcga taatcgccag tactacgcga atcgcttcgc
     1741 tgtcacccac cggtgcgtag aggaacagac gcatcaccac accgaagata gcgattttgc
     1801 tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg tctggcgtcc
     1861 acaggtggaa cggcaccaga gagagtttga agccgaggcc aacaatcatc atgccgaaac
     1921 ctgccagcaa cagcggctcg ttgagcatac cgtcgccgag gtttttaccc agcgcgacaa
     1981 acgacaggtc gccagactgc gcatacacca gcgccatacc gaacagcagg aaagaagacg
     2041 ctgcggcaga aaggatggtg tatttgatac tggcttccag cgaacgtttc tggcggaaag
     2101 cgtaaccgac caggccaaac agcggcaaag agatcagttc aataccgagg aacagagacg
     2161 ccagatggtt tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac accaacaggt
     2221 agaactcatc cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg
     2281 cgaggctcgc caacaatacc agcccggtgt aaagcatggc gaaaccatca acgcgcatca
     2341 gcggcgtaac gtccatagcg cccgcctggc caacaaacca gagcgaaacc agcgccgcgt
     2401 taagcccaat aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga
     2461 gcatcacaac caccaccgtc aagccgacga tcagcaacgg tagcagtgcg atcaggtttt
     2521 gtggagttat tgtcatggcg atttacggcc ttgtagtagt aacggaatta acaaaccact
     2581 gctggatatt gccaatcgcg gagtgcgagg tatccagaat cggctgcgga tagaagccca
     2641 gcagtaccag cagcaccacc agcaacagga tcataaacag ctcacgcagc gacatccctg
     2701 gcagttcctg gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga tgcaacatcg
     2761 ccagcgaata aacagatgca aagaccagcc caaaggtaga gataacggta atcaccggga
     2821 caacctggaa gctgccgaac agaatcataa attcgccgac gaagttaccg gtgccaggca
     2881 tcccaagcgt tgccaccgca aagaacagcg acagtgctgg cagccatttc atcttgctcc
     2941 acagaccgcc catcatgcgc atgtcgcggg tatggatacg ttcataaagc tgaccgcaga
     3001 gaataaacag acccgccgcc gacaagccgt gcgcaatcat ctggattacc gcgccctggt
     3061 aggccaactg gctgccggtg tagattgcaa tcagcacgaa gcccatgtgg gaaaccgagg
     3121 tgtaggcgat cagacgtttg atatcggtct gagcgaaggt catccacgca ccgtagaaga
     3181 tgccgataac acccagccac atagcgattg gcgcgaactc tgccgacgcg ttcgggaaca
     3241 gcggcaggga gaaacgcaac aaaccgtaag cggcggtttt cagcaagatc cccgcgaggt
     3301 ccacggaacc ggcggtcgga gcctgggagt gcgcatccgg cagccagcca tgcagcggaa
     3361 ccaccggcat tttgactgcg aaggcgatga agaagcccag catcaacaga tattccacac
     3421 cactggacat tggcgtattc agcagctctt catagttgaa ggtccagacg ccggtcgcat
     3481 tgtagtgaac aaaaaccagc gccaggatgg cgatcaacat caccagacca ctcgcctggg
     3541 tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt accgtcagag gctttatgcc
     3601 cccacagtgc gatcaggaag tacatcggca ccagcatcat ttcccagaag aagaagaaca
     3661 ggaacatgtc gatggcaagg aacacgccga taacgccgcc caggatccac atcaggttga
     3721 ggtggaagaa gccctgatat ttttcgatct ctttccacga acatagtacc gccagcacac
     3781 cgagcagacc ggtcagcacg accatcagca gcgacagacc gtcaatggcg agatgaatag
     3841 agataccaaa acgcgggatc cacggcatgt cgaattcaga ctgccactgc ggaattccgg
     3901 cggattgcgt cagtgaataa ccgccctgca accacagttg cagcgacagc gccagcgtca
     3961 atcccatggt gatcagcgcg atccagcgtg gcaccttgac gccaaagcgt tcggtctgcc
     4021 agcacaggaa gccgccaata aagggaatta atattagcca gggtagtaac atggcgatct
     4081 ttattccttg taaaagtccc gtcaggaccg gattttcaac gaattctcac gacagaaaat
     4141 tctcttcgga ctgggggtag tgtcggatgc gccgcgaagg cagcttatcc gacctacgtt
     4201 cttatatgcc caggggcaat cccacaatcc ttaactcaac gcagtaccat caacagtgcc
     4261 agcaccacga ccgcaccgat gctcatggat gccacatacc agcgcagata accgttctcg
     4321 cttaacagca gacctttacc tgcaaagcgg gaaaaaaccg ccgggatgtt catcattgag
     4381 ttcagcggat cgcgtttcag caaccaggca atacccagga acggcttgac gaacactttg
     4441 tcgtacagcc agtcaaagcc ccaagcgttg taccaccagg tactcagcag gcggcccggc
     4501 gcactgttgg cgatggaggt caccagagaa cgtttaccca gccacagcca ggctgccagc
     4561 agaatgccga ccaccgcgac cacgccagag gtaatttcca gggtcaacat gctgccgtgc
     4621 gccagttccg tcgtttgcgg aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg
     4681 aaggtggaaa ggatcagcag cacaatcagc ggcaggctgt gagttacccc tttcacggcg
     4741 tgagcgtgaa tttgttcttt tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc
     4801 gaggtcataa acgcaccgac cagacctgcc accatcagat tgatatgacc attcgccatc
     4861 gcacccgcga ggatctcatc cttactgaag aagcccgcag tgaccagcgg cagtgccgac
     4921 agtgctgcgc cgcccaccag gaagcagaga taaaccagcg gaatagattt acgcagaccg
     4981 cccatcttga agatgttctg ttcgtgatgg caggccagaa tgacggaacc ggatgccagg
     5041 aacagcagcg ctttaaagaa cgcgtgagtc atcaagtgga aaatcgccgc atcccatgcc
     5101 tgcacgccaa gcgcgaggaa catgtagcca atctggctca tggtagagta agcgagaaca
     5161 cgtttgatgt cggtctgtac cagcgcggca aaaccggcca gcagcagcgt aaccgccccg
     5221 acaataccca ccagatgcag aacttccggc gtcatcagga acaggccgtg ggtacgggcg
     5281 atcaggtaga cacccgcggt taccatcgtt gcggcgtgga tcagcgcgga gacaggcgtc
     5341 gggcccgcca tcgcgtcagc aagccatgtc tgcaacggca actgcgcaga tttaccgacc
     5401 gcaccgccca gcagcatcag cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc
     5461 gctggtgcca gttccaccat ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg
     5521 aacagtgcga aagcgaggaa aacgtcaccc acacgggtca cgacgaacgc tttcattgct
     5581 gctgcgccat tcttcggatc ggtgtaatag aaaccgatca gcagatagga gcacaggccc
     5641 acgccttccc agccgaggta catcagcagc aggttatcgg cgagaaccag aactaccatg
     5701 ctggcgatga acaggttggt gtaggcgaag aagcgagagt agccctcttc accgcgcatg
     5761 taccaggagg cgtacatgtg aataaggaaa cccacaccag tgaccaccga gagcatggtc
     5821 agcgacaggc cgtccagcac caggttaaaa ccgatgttaa agtcgcctac cgacatccac
     5881 gtccacagcg gctggctgta tgcctgctcg ccgttagcga agaaatcaac gccgataaag
     5941 gcggttacca gcgccgccag gcccacagag ccaacgccga cgatcgccga aacgttttca
     6001 gaccagcgcc cacgggagaa cgccagcagg acgaagccaa tcaatggcaa aataatggtt
     6061 aaggcaagca tgttcatccg cgcatctcac ttactgaatc gatgttcagg ttctggcgac
     6121 gacggtgaag ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga
     6181 tggcgagaat gtacatcacc tgaccgtcgg tctgacccca gtagcttccg gcgaccacaa
     6241 aggccagcgc ggaggcgtta atcatgattt ccagaccaat cagcataaac agcagattgc
     6301 gacggataac cagaccggtt aagccaagaa cgaataagat tgccgcgagg atcagtccat
     6361 gttgtaaggg gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt
     6421 gctcagcact tcacccgcac gctcttcacg accgacgtgg aaggccacaa ccagacccgc
     6481 gagcagcagc atagaagcca gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc
     6541 cactgcttta gcactgattg gcgtaccgtc gataccctga tcgttaacac cgaggatggc
     6601 gtaaacaatc accaccagca tgatggccga caaaattgcc gggccaatcc acacctgcgg
     6661 tttcagccac tggcgttcct gttcgatttc tgaaccgccc aggttgagca tcatcaccac
     6721 gaacacgaac agcaccataa tggcacccgc gtagacgata atttccagcg caccggcgaa
     6781 gtaagcgcct agtgagaaga acaccccgga aatcgccagc agcgaaataa tcaggtacag
     6841 cagtgcgtgt accggattgg tatgggtgat cactcgcaag gtcgcaagta tggctatcag
     6901 gccacagata taaaaagcga actccattgc ccctctcctt acggtaacag gctcttgacg
     6961 tcgataggct tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat tgccatacct
     7021 gccatccggt agaagttata ttccgggtat ttgcccggac cggagatcag cagatcctct
     7081 ttctcgtaaa ccagatcctg gcgcttgtat tcccccattt cgaaatccgg ggttaactga
     7141 atcgccgtgg tcggacaggc ttcttcgcac agaccacaga aaatgcagcg tgagaagtta
     7201 atgcggaaaa attccggata ccagcgaccg tctttggttt ctgctttttg cagcgagata
     7261 cagccgaccg ggcaggctac cgcacagagg ttacaggcta cgcaacgctc ttcgccgtcc
     7321 gggtcgcggg tcagaacgat acgaccacga taacggggcg gcagatagac cggctcttcc
     7381 gggtacattc gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca gatactacga
     7441 acctgggtgc cgaaacctac taacaattct tttaaggtca tggtcttatt gccccttatt
     7501 gcgcctgcca gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc
     7561 agattttcca gccgaaggac attacccggt cataacgcgg acgcggtaac gacgcacgaa
     7621 tcaaaatgaa catcatcata aagaacgcgg ttttcagcgc gaaccagatg aatggcggta
     7681 acaacgggcc ttgccagcca ccgaagaaca gcgtcaccat caatgcagag atggtcacaa
     7741 tcccgatgta ctcacccacg aagaacagac cgaacttcat accggaatat tcaatgtggt
     7801 aaccatccgc cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg tgacacaccg
     7861 ccacgcccgc gatggcaaag gtaataaaac caaagaattg cgggatgacg ttccacacat
     7921 gcgcctggct gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca
     7981 tcagggaaag cccgaggaac acttcgtagc tcagggtctg cgcagaagca cgcatcgcac
     8041 ccagcagcga gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca
     8101 gacctgccat catcaggaag aacaaaatcc cgatgttcag gtcggcaacc acccaacccg
     8161 gactgactgg cacaattgca aaggccagca gcagcgaggt aaaggcaatc atcggtgcca
     8221 gggtaaagat gacgcgatcc gagaatttcg ggatccagtc ttctttaaag aacattttga
     8281 tcatgtccgc aaccagctgg agcgaaccgc cccagccaac acggttaggt ccataacggt
     8341 tctggaacag acccagcagg cgacgttcgc caaagctcat gaatgcccca caggtgacaa
     8401 ccaccagcag gatcaccacc gctttgagga tggtcagcag gatctcaatc agttccggtg
     8461 atatccaact cattgttgtg cctccttgag atcctcaaga tgcgcgccag ccagcaccgg
     8521 agcaatgccg gacataccca tcggcaagcc cacctgccct gccgtcagtc cttcggcgat
     8581 ttcaaccggc agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt
     8641 cacacccaac ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg gcatacggct
     8701 ctggaagacc ggagcacgct gtgacaattc atcgctgcca aacaggtggt agtacggcgc
     8761 gatacgccat ttcccgtcct gcggctggaa gcgtgccggt acgctggtga agtaatccag
     8821 accgttttcg ctggtttcaa acagacgcac gcccggatcg ccaaagcgca gtttgccgcc
     8881 cacttcgtcc tggaatttgt tccacgcctg cggggagttc cagcccggcg cccaggcaaa
     8941 cggcacttgc gaacggtgcg cggtcggctg gttgttacct tccatcgaga aggtgaacat
     9001 ggtgtcaata tcctgcggct gacgcggctc atgaacgctg atattggcgc gcatggcggt
     9061 acgaccgctg taacggtgcg gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc
     9121 atccggcgca gcatctttga tacccgccag ttccgggatt ttcgccacaa cagcgtcaat
     9181 tacatggtcg agctgcgtcc agtccacttc acggctcagc aaggtgctgt gcagcgagtg
     9241 cagccagcgc cagctttcca gcatgacagt tttgctgtcg taataagccg gatcgtaaac
     9301 ctggaagaaa cgttgggcgc gaccttcatt attaattacc gtaccgtcgc tttcagcaaa
     9361 gctggcagag gaaagcacca gatgggcgtt ttccatgatc gctgtgcgtt gatgatcaac
     9421 caccatcacc agcggcgctt tagccagcgc agcattcacg cgggtagcag aagcgtaacg
     9481 atgcagatcg ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa
     9541 cgcttcttca agcgaaccgc cacccataat gcccagcccc atgctgttga cggaacgggc
     9601 aatcatggtg ataccaacgt cagcgccgcg acctttcagg gctttcgcga cgttagccgc
     9661 agcctgaatc acctctatgc taccggcgtt cgtcccggag ataatcaacg gtttcttcgc
     9721 acctgccagt gcctgcacga tgacgtcgat tttgttttgc agctcaggtt cgataccgtc
     9781 aaccgctggt gcggagttat ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc
     9841 ttcaaccggt gcgcggtaag tccacgccgc gatatcatcc agacgagtgt catcaacgtt
     9901 ggtaacaaac agcggatgct tcgcacgttg accgatgttg aggattgccg caatctgcca
     9961 gtcagccact ttctgtgctg cagccatttc gcgcgcttta cctttcaccg cctgacgcac
    10021 tgccagtgcg acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca gtaccgcatc
    10081 gtaagattcg atttcgcgca gagccggagt ataaatgccg ccttcacgca gcactttcag
    10141 cgccagttgc agacgttcct gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc
    10201 caccagttca cgcagcgcaa agttgctttc cacgctggca cgcggagaac caataccgat
    10261 cactttcttc gactgacgca gaatatctgc cgcgccctgc attgcctgtt cggcgttgag
    10321 ggtaatgaaa tcatcgccac gacgctgtac tggctgacgc ggacgatcct tcaggttgac
    10381 gtaaccgtaa ccgaaacgac cacggtcgca gaggaagtag tggtttaccg taccgttgta
    10441 acggttttcg atacgacgca gttcgccgta acgttcaccg gggctgatgt tacagccgat
    10501 ggaacattgc tggcagatgc tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc
    10561 ggagtgcgtt ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt taccggaaaa
    10621 ttcgctttcc agcgtgccgt cttccgggcg accgaagtag acgttgtcgt gcgcgccata
    10681 aacgcccaga tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc ggtagcaggc
    10741 gatgcagcgg ttcatttcgt gagagatgaa tggccccaaa tcctgattac ggtgggtacg
    10801 tttggtgaaa cggtagcgac ggaagctgtg tccggtcatc acggtcatat cctgaagatg
    10861 gcagttaccg ccttcttcac ataccggaca gtcgtgcggg tggttggtca ttaaccactc
    10921 gaccacactt tcacggaact gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc
    10981 ggaagccggt gtcatacagg acatcaccag gcgaccacgc gtgtcttccg cgttttggta
    11041 ttgcttcacc gcacactggc ggcaagcacc gacgcttccc agcgccggat gccagcaaaa
    11101 gtaaggaata tcaaggccca gagacagaca agcttccagc aggttgtccg ctccgttgac
    11161 ctcgtattct ttgccgtcta catgaattgt agccattagc atgcttccag ttttctcggt
    11221 cagggaccga gcgttaatcg aaattcggtt accagcgctc tttcagcagg ttcggctgaa
    11281 tcccattaat caaatgggta ttgctgaacg gctgtttgat tcccgcctca aattcttcgc
    11341 ggaaatattt gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag
    11401 ttttacccgg gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg cccggctggc
    11461 cttcgccacg ctccagcgca cgcagaattt tcacgctcca cggcagaccg tcgcggcacg
    11521 gcgtacacca gccgcaggac tcacgggcga aaaactcttc caggttacgc accagcgaca
    11581 ccatgttgat ctcatggtca accgccatcg ccagcgccgt acccagacgg ctgcccgctt
    11641 taccgatact ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg aagtcagtcc
    11701 ccgcgccgcc tggctgccag gctttaaatt tcagaccatc acgcatacca ccggcgtaat
    11761 cttcgaggat ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc ggatttttca
    11821 cccgaccgga gaagcccatc agcttggtgc cagcatcttt acttttcgag atgttctgat
    11881 accactccac gccgttagcg aggatcgccg gaacgttaca cagggtttcg acgttgttga
    11941 cacaggtcgg tttgccccat acgccggagg ttgccgggaa ggggggtttc gagcgtgggt
    12001 tagcacgacg cccttccagg gagttgatta acgctgtttc ttccccgcag atgtagcgcc
    12061 ctgccccggt atggacgaac agttcgaagt cgaaacctgt tcccataatg tttttgccaa
    12121 gcaagcccgc ttcagtggct tcggcaatgg cgcggcgcag atttactgcc gcttcgatat
    12181 attcgccacg caggaagatg tagccacggt aagctttcag cgcaaacgcg gagatgagca
    12241 taccttccac cagcaggtgc ggcagttgct ccatcaacag gcggtcttta taggtgcccg
    12301 gctccatttc atcggcgtta cacagcaggt aacggatgtt catggattcg tctttcggca
    12361 tcaggctcca cttcaggcca gtcgaaaagc ccgcgccgcc gcgccctttc agaccagcgt
    12421 cttttacctg attaacgatt tcgtccggag acagcccggt cagcgcctta cgcgcccctt
    12481 cgtaaccgtt tttgctgcgg tattcgtcca gccacactgg ctgtttgtca tcgcgcagac
    12541 gccaggtcag cggatgcgtt tcgggagtac ggataatgtt tttcatttat accgctccag
    12601 cagttcaggg atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt
    12661 tggcccttta tcacagttcc ccaggcagca agttggcagc agcgtaaagc ggccatcaaa
    12721 tgtcgtttgc cctggtttga tgttcagctt tttctcgagc gccgcctgaa taccctgata
    12781 cccgttgata tgacagacca cgctgtcgca ataacggatc acatggcgac caaccggctg
    12841 gcggaagatc tgactgtaga acgttgccac accttcgacg tcgcttgccg gaatacccag
    12901 cacatcggcg atcgcgtgga tcgcaccatc cggcacccag ccacgctgct tctgaacgat
    12961 tttcagcgct tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat
    13021 cgcttcacgc tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg
    13081 cataattagc ggtccacatc tgacataaca aaatcgatac tgcccagata aacaatcagg
    13141 tcagacacca ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc
    13201 gtgcggatac gagtgcgata actcatggtg ctaccgtcgc tggtcaggta gtaactgttg
    13261 atccccttgg tcgcctcaat catctggaaa gattcattgg caggcatcac cggcccccac
    13321 gacacttgca ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc
    13381 ggcggcgtgg tcagcgggtg atccgctttg aacgggcctt ccggcatgtt gttgaggcac
    13441 tgctcaagaa tgcgcagact ctggcgcagc tcttccactt tcagcattac gcgggtgtag
    13501 cagtcagaaa caccaccacc aaccgggatt tcaaagtcga agttttcata gccagaataa
    13561 ggacgcgcct tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc gccagtggtg
    13621 ccccactcca gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg acctttcaga
    13681 atggtatttt gcagcgccgc tttctcgtaa gacgccagac gtttcggcat ccagtcgagg
    13741 aactcacgca gcagacgatc ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg
    13801 aaccacgccg ggtgcatacg gaaacccgtg atcgcttcca ccagatcgta aattttctga
    13861 cgatcggtaa aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat aaaggtcgag
    13921 atgtacagca ggtggctgtt gatacggaac agttcggaga gcataacgcg aatgacgtta
    13981 acgcgatccg gcacggtgat cccggccagt ttctctaccg ccagcacgta aggcatttcg
    14041 ttaacgcagc cgccgaggta ttcaatacgg tcggtatacg gaatgtagct gtgccaggac
    14101 tggcgctcgc ccattttctc cgcaccacgg tggtggtagc cgatgtctgg tacgcagtcg
    14161 acaatctctt cgccgtcgag ttgtaaaacg atacggaaag ccccgtgcgc cgacgggtgg
    14221 ttcggaccga ggttgaggaa catgaagtcc tcgttttcgg tgccgcgctt catcccccac
    14281 tcttccggtt tgaaggtcag ggcttccatc tccagatcct gtttggcttt ggtcagctca
    14341 aacggcgaga attcggtagc gcgcgccggg taatctttac gcagcgggtg acctttccag
    14401 gtttgcggca tcatgatgcg gcgcaggttc gggtgaccgt cgaaagtaat gccaaacaga
    14461 tcccaggttt cacgctcata ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg
    14521 tgcaggtcgt tttctgccag cgccaccttc agcatgatgt cgcggttacg atcgatagaa
    14581 atcagatggt agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga
    14641 cgttcgtcca tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag tttctttaag
    14701 aaatcgccaa cttccagtaa ttgttcacgc ttgatccaca caacgggaac cccggtgcga
    14761 gtcgcctgaa cagtaaaggc atccggccca aaacggttgc gcagttcgcc aattaccgga
    14821 tcatcaagat gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg
    14881 ttcaccattg caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg aaatagattt
    14941 aggaataagc gaagaaaaat ccctttgccg acaggcgcaa attaaatctc gtcaggtgta
    15001 cgcaggttgg ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg
    15061 gcgcgataaa cgccctgatc gccaaccacc caggagagcg gacgacgttc tttgccgata
    15121 gattcctgca acagcatcag tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg
    15181 atatacacat caaccgggat gaatttatcg acgccctgca caacggaata aatatcgtac
    15241 ataccaccag agttggcaca ggcacccatt gagataaccc attttggttc cagcatctgg
    15301 tcatacagac gctgaataac cggtgccatt ttggtaaagc aggttcctgc aaccaccatc
    15361 aggtcagcct gacgcggcga agcacgcaat acttctgcgc caaaacgcgc cacgtcatgc
    15421 accgcggtaa acgaagtcac catctcaacg taacagcagg aaagaccgaa gttatacggc
    15481 caaattgagt ttttacgacc ccagttaacc atgtcattga gcttgcccat aaacacgttt
    15541 ttgttaactt cttgctccag agggtcggtt acgatctcct gcttttgcag ggggtaacgg
    15601 tcgttctcac cgttgggatc tatgcgggtg agcgtataat ccatcttaat gcctcgcggt
    15661 tagcgttgac gattagcgat actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc
    15721 gcgggcgtcc agtccagcgc gccaatacgc accagataaa ccagacctgc cagtaacaca
    15781 aaaataaaaa ttgcagcttc cacaaagcct acccagccgc tttcgcggat agaggttgac
    15841 catgcgaaca gatacagcgc ttcaacgtcg aagataacga agaacatggc caccagataa
    15901 aacttggcgg acaggcgtaa gcgggcggag ccgaccgagt cgataccgga ttcaaacggc
    15961 acgtttttcg acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg
    16021 caacacaggc caatggcaac gataagaaag atagcgaatg cccagtgatg agcgatgact
    16081 tcagtggatg ttgacatact cattgcttac tcatcaaaag tagcgccaga ttcactgttc
    16141 ttcacggcag atggacgcca catcgattca tggggaggaa taaaaaaaac cttacaataa
    16201 ctgtagaaat tcttttatac agctaattga tgtggtcttt tactcctttc tataaccttt
    16261 tgtcaacttt aacaaaagct tcttcacatt agtttacata atatcaacac cattagcatt
    16321 taatgccctt tcaccccaga tccttgacga ctccaggata attagatgtt gttgaatcgt
    16381 gtccgttgtg aagcaatgga aaaaatacgg gtctattttg acaggaattt gtgtcgattc
    16441 ctccccccaa aagagagtat tttcttgatc tgtgacacgc ttttgttatt ccataacaaa
    16501 aacgcagcaa caaatttacg tattttttaa catcattgta gcaggtgatt tttttcaggc
    16561 gattatttgt gcattcggga cgtgaatctc tggtgattga aaaataaaca gttttgtacg
    16621 ttctgcacta tgcgaggaag gatttttact aaaaaaaagc cgctggggtt taaaacaccc
    16681 ccagcggctc gttttttaca ctattgtctc aggaattatc tatcgtccgt cgattactcg
    16741 atatcccttt caatcaacaa tgaatcatcc ccttccggag cagacattgg gctgtattgc
    16801 cacggattat ggtagctctc catcgcctga tagatcacct gggccagctc attattactg
    16861 gacgggtcat agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca
    16921 ctcagcacac gcaggtccgg gctcatcatc tcaaccggcc ttgccgtcac gccaagaccg
    16981 gctttcactg ccgcacgaac ggccggaagc gtcgaggcga cataagccag tcgccatgga
    17041 atatctactt tattaagcgt cgccagcacc atatcgcgaa acggacttgg atcatccagc
    17101 aatacaagag ggatcggctc gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt
    17161 gttggcgagg tacgcaggtt caatgcctta aacgctgaag gacgatgggt ggttaccatc
    17221 aaatccactt cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca
    17281 tccagcgcta atttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta
    17341 tcggcagatt catctgaagc accgatagtt aacacgccct gaagattact gaacattaat
    17401 gatgagcagg cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg
    17461 ccatgttcag ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt
    17521 tgttccagac gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct
    17581 gcggcagcaa aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg
    17641 aggttaatta tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact
    17701 gagctggtta atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg
    17761 cagtttcatc cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga
    17821 ctcacattgt tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca
    17881 tatctattaa gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa
    17941 atgtgagtcc ttgttccact ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac
    18001 agcaatggtg aagcggcatc aagagcaggg atccgtcatt tatgcgagca ttttacccca
    18061 aaaacctttt atttataagg gtcattgcga attatctgat gcaaagttat gttatgttag
    18121 gcaaagtaat cttctattat taataagcac atcaaaacct ttttgaatat taaataataa
    18181 ttaattagca tcatcctcat tcattaattc cgcttaacaa tagtttcaca ccttaccccc
    18241 attgcgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga aatatcatca
    18301 tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct tacagataaa
    18361 aaaaccagac ttgcaattaa gaattagaac aattcactat ataacattgc atgtaaagca
    18421 tatacacctc attattttgt cattattaag ttattaacag cacaatcgag ccttcccctc
    18481 tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac attgttctaa
    18541 gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt catgtccccc
    18601 attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc ggtgctgaaa
    18661 gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat cggcaaccca
    18721 gccccattcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg caacctgcct
    18781 accgctcaag ggtattgcga ttccaaaggg ctttactccg cgcgtaaagc catcatgcag
    18841 cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat cggcaatggt
    18901 gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga cgaaatgttg
    18961 gttcctgcac cagattaccc actctggacc gcggcggttt cgctttccag cggtaaagcg
    19021 gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga tattcgcgct
    19081 aaaattacgc ctcgtacgcg tgggattgtt attatcaacc caaataaccc aaccggcgcg
    19141 gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca taatctcatt
    19201 atcttcgccg atgaaattta tgacaaaatt ctctacgacg atgctgagca tcactcaatt
    19261 gcgccgctgg cacctgacct gctgaccatt acctttaacg gactgtcgaa aacgtaccgc
    19321 gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca cgccaaaggc
    19381 tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt tcctgcgcaa
    19441 cacgccattc agaccgcgct gggtggttat cagagcatca gtgaatttat tacccctggc
    19501 ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat tccgggcgtt
    19561 tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc caaacgcttt
    19621 aacattcatg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa agttctgttg
    19681 gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt cacgctaccg
    19741 cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc tggttatcat
    19801 cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagtgttatc ccgccacaat
    19861 gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt tttgcccatc
    19921 tctcccgcct gaaactcatt aaccgctggc cgctaatgcg caacgtgcgg acggaaaatg
    19981 tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc gctggcagct atcaaaaatc
    20041 ggaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg taccacgatg
    20101 cctcagaagt gctcaccggc gatctcccta ctccggtgaa atacttcaat gcgcaaatcg
    20161 ctcaggagta caaggctatt gaaaaaatcg ctcagcagaa actggtcgat atggttccgg
    20221 aagaactgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc gatgaagaaa
    20281 aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg gaagaactcg
    20341 cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg cttgaagcgc
    20401 gtcgcagcca ggagatggac tacttcatgg aagtatttgt tcccagcttc catctttcgc
    20461 tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc gcatcagtca
    20521 ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg ggatcatcac
    20581 cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa agtcactgaa
    20641 gctgtaattt cccggaccta aaaccagggt gttaacaggt gaagacaccg gtgtcataaa
    20701 ggcggcggat gctgccatcg ccacgaccat cgcaaatgga taaggcgaca ctcccatcgt
    20761 tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat tagagataaa
    20821 cagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg gcccataacc
    20881 gccgccaata tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt tctgtaatgc
    20941 cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa tggatttata
    21001 ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga tgatagcggc
    21061 aacaggatta ggaatttcat ttgtcagcat taacgccacc attagcacca gacagaaaat
    21121 ggcatggggt gcctggctgt gcgcgggcga tgcttcactc acctcttccg gtaagttcag
    21181 cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt tacccacaac
    21241 caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca gcgccacgcc
    21301 attgcgcttg agccccacca cattcagtcc gtagcgggta cgaaaaccaa tttcgcgcac
    21361 cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc ccacatcaag
    21421 ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt gctcactaca
    21481 aaattgccgg agatcgacat cagccgcaga catatcaata agcaaaacgt cacgcgcgcg
    21541 aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc agcgttcaac
    21601 accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc gttgacctat
    21661 cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc gatattcacg
    21721 gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct gggtatcccc
    21781 tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca cgaccaggcc
    21841 aataggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca attcactgtt
    21901 gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgctaatca gcccggcaaa
    21961 acttaacggc atcatcagac gtgacggcga agtttgcata cgcatggcaa cgcttaacac
    22021 cacgggaata aagatagcga caacgctggt tgaactcata aacgcgccaa gccccgcgac
    22081 ggtcagcatc aacaaaacca acattttgat ttcactgttg cccgccactt tgaccagcca
    22141 tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa taaacaaggc
    22201 ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttcgggga ccgtcagcgt
    22261 tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac gcactctgcc
    22321 cgtcgcaaac aagacgatgg caacggccag taatgaaaga acccaaatca attcaccgtt
    22381 cacaacttat ccttgttaat tgagggggat gacttgattc tgccataaaa aagccccgac
    22441 gagacggggc taaatcatga tcaggtgttt cactgaataa taacatcgcc atttggctgt
    22501 ttggtcacag taatttgctc cagactgtgg aggaccaaat cgacctcatt caggcgcggg
    22561 gtatctgccg gagcgttaac cgcaatgaca tggcaacccg ccgccaggcc agaaagcacg
    22621 ccagcgggag catcttccac caccacacac tcctgcggcg caagccccag cagctgcgca
    22681 cctaacagat acgcatcagg ttctggtttt ccgcgcttca ctcgctcagc ggttacaaac
    22741 acctctggtg cgggaagccc cgctatttta tggcgcgctc gcgctaccgg catggagcca
    22801 gaagtcacaa tggcccacgg aatacctgct ttattcaaat gattgagtaa ggcgattgcc
    22861 ccaggaagcg cggtaatacc ttcggtttcc gtggcctcga tttgctccag acgcgtaaac
    22921 tcggcggcaa tatcagcctc ggatttgccc gccataaaat ggcgcaaaga ggtgatcgcc
    22981 tgtttaccgt gaatgaaagc cagcacctct tccggcgcta acccatgacg tctggcccag
    23041 ttgctccacg cccgttctac cgcaggcagg gaatccacca gcgttccatc aagatcaaac
    23101 agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg ataatttgat taatttcgtt
    23161 ggcgctcaaa tggtactgac gcgggcaggc atgccacaca ttaagcatgc gctgatattt
    23221 ttcccacatt ggcgtctggg cgttaaaacc atgagtccca gcgtcaaaat gggtatagcg
    23281 cccttccaca ttaaccataa agcggacata accgaggtaa cgtgcttcag tggcggcgtc
    23341 aaagccgagg aaggtgacac gacgttcatc gatggattgc tggtcctgca aattagacca
    23401 ggaaacatac aacgcatgat acatctccat aatgtcgatg atagtgcggc aggtttcttc
    23461 tttcagctcg ccaaactcgc gatccaattc acgcatctgt aatccgtaac cacgctcaat
    23521 aattgtttgc aggcgacggt aacgttcagc atttgccgga tcgagcatag tcatcatctt
    23581 gtactgatta gacaaaataa gacgttgcgc gttggtcatt tccattgttg actcctgtat
    23641 cactctacta cggtgaaaaa aaagaaggct gagtatgcct tcttttatat gcgtaatcag
    23701 gggtcaatta caaatcatca aggaaagttt tatccagttg tttgaaggcg cgcttaagcg
    23761 tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc tgtccagact
    23821 cctgcaattt accgcgaact tcataaaacc agttaaggat tgcagggggt aatggcgtta
    23881 cagaacgctt gcccagccac cacaatccct gcatgggtaa acttaaggcg aacagtgcag
    23941 tggcaactgc cggcccaagc agaccgccca gggcaatctg ccagcagaga gtaaatacgg
    24001 cgatcggcgg cataaaacgg atcgcataac gcgtcatctt gataacgcga ttttcgacaa
    24061 agaccggggc aaggcgtttt tccagtggcc acgtctttga gtaatgctgt ccgcggcgaa
    24121 acaagctaaa aaaattaaca gaacgattat ccggcgttga catgcttcac ctcaacttca
    24181 catataaaga ttcaaaaatt tgtgcaaatt cacaactcag cgggacaacg ttcaaaacat
    24241 tttgtcttcc atacccacta tcaggtatcc tttagcagcc tgaaggccta agtagtacat
    24301 attcattgag tcgtcaaatt catatacatt atgccattgg ctgaaaatta cgcaaaatgg
    24361 catagactca agatatttct tccatcatgc aaaaaaaatt tgcagtgcat gatgttaatc
    24421 ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg
    24481 tatcaattat aggtacttcc atgtcgagta agttagtact ggttctgaac tgcggtagtt
    24541 cttcactgaa atttgccatc atcgatgcag taaatggtga agagtacctt tctggtttag
    24601 ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa aatggacggc aataaacagg
    24661 aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc gttaatacta
    24721 ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac
    24781 acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca
    24841 aagatgcagc ttcttttgca ccgctgcaca acccggctca cctgatcggt atcgaagaag
    24901 ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc
    24961 accagactat gccggaagag tcttacctct acgccctgcc gtacaacctg tacaaagagc
    25021 acggcatccg tcgttacggc gcgcacggca ccagccactt ctatgtaacc caggaagcgg
    25081 caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac ctgggcaacg
    25141 gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg ggtctgaccc
    25201 cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc
    25261 acctgcacga caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt
    25321 ctggcctgct gggtctgacc gaagtgacca gcgactgccg ctatgttgaa gacaactacg
    25381 cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca
    25441 tcggtgccta cactgcgctg atggatggtc gtctggacgc tgttgcattc actggtggta
    25501 tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg caaactgggc gtgctgggct
    25561 ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca
    25621 aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag
    25681 acgcgagccg cctgactgcc tgatttcaca ccgccagctc agctggcggt gctgttttgt
    25741 aacccgccaa atcggcggta acgaaagagg ataaaccgtg tcccgtatta ttatgctgat
    25801 ccctaccgga accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc gtgcaatgga
    25861 acgcaaaggc gttcgtctga gcgttttcaa acctatcgct cagccgcgta ccggtggcga
    25921 tgcgcccgat cagactacga ctatcgtgcg tgcgaactct tccaccacga cggccgctga
    25981 accgctgaaa atgagctacg ttgaaggtct gctttccagc aatcagaaag atgtgctgat
    26041 ggaagagatc gtcgcaaact accacgctaa caccaaagac gctgaagtcg ttctggttga
    26101 aggtctggtc ccgacacgta agcaccagtt tgcccagtct ctgaactacg aaatcgctaa
    26161 aacgctgaat gcggaaatcg tcttcgttat gtctcagggc actgacaccc cggaacagct
    26221 gaaagagcgt atcgaactga cccgcaacag cttcggtggt gccaaaaaca ccaacatcac
    26281 cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta ctcgcccgga
    26341 tctgtccgag attttcgacg actcttccaa agctaaagta aacaatgttg atccggcgaa
    26401 gctgcaagaa tccagcccgc tgccggttct cggcgctgtg ccgtggagct ttgacctgat
    26461 cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg accatcatca acgaaggcga
    26521 catcaatact cgccgcgtta aatccgtcac tttctgcgca cgcagcattc cgcacatgct
    26581 ggagcacttc cgtgccggtt ctctgctggt gacttccgca gaccgtcctg acgtgctggt
    26641 ggccgcttgc ctggcagcca tgaacggcgt agaaatcggt gccctgctgc tgactggcgg
    26701 ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta ccggcctgcc
    26761 ggtatttatg gtgaacacca acacctggca gacctctctg agcctgcaga gcttcaacct
    26821 ggaagttccg gttgacgatc acgaacgtat cgagaaagtt caggaatacg ttgctaacta
    26881 catcaacgct gactggatcg aatctctgac tgccacttct gagcgcagcc gtcgtctgtc
    26941 tccgccagcg ttccgttatc agctgactga actggcgcgc aaagcgggca aacgtatcgt
    27001 tctgccggaa ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg ctgaacgtgg
    27061 tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg cagcgtctca
    27121 gggtgtagaa ctaggtgcag gcattgaaat cgttgatcca gaagtggttc gcgaaagcta
    27181 tgttggtcgt ctggtcgagc tgcgtaagaa caaaggcatg accgaaaccg ttgcccgcga
    27241 acagctggaa gacaacgtgg tgctcggtac gctgatgctg gaacaggatg aagttgatgg
    27301 tctggtttcc ggtgctgttc acactaccgc aaacaccatc cgtccgccgc tgcagctgat
    27361 caaaactgca ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc tgccggaaca
    27421 ggtttacgtt tacggtgact gtgcgatcaa cccggatccg accgctgaac agctggcaga
    27481 aatcgcgatt cagtccgctg attccgctgc ggccttcggt atcgaaccgc gcgttgctat
    27541 gctctcctac tccaccggta cttctggtgc aggtagcgac gtagaaaaag ttcgcgaagc
    27601 aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc tgcagtacga
    27661 cgctgcggta atggctgacg ttgcgaaatc caaagcgccg aactctccgg ttgcaggtcg
    27721 cgctaccgtg ttcatcttcc cggatctgaa caccggtaac accacctaca aagcggtaca
    27781 gcgttctgcc gacctgatct ccatcgggcc gatgctgcag ggtatgcgca aaccggttaa
    27841 cgacctgtcc cgtggcgcac tggttgacga tatcgtctac accatcgcgc tgactgcgat
    27901 tcagtctgca cagcagcagt aatctcgtca tcatccacag ctttgcgctg cggatatctg
    27961 aaccggaaat aatcactatt tccggttttt tattctctta atttgcatta atcctttctg
    28021 attatcttgc ttaactgcgc tgcatcaatg aattgcgcca tctcactttg catacttacc
    28081 actttgtttt gtgcaaggga atatttgcgc tatgtccgca atcactgaat ccaaaccaac
    28141 aagaagatgg gcaatgcccg atacgttggt gattatcttt tttgttgcta ttttaaccag
    28201 tcttgccacc tgggtagttc cggtggggat gtttgacagt caggaagtgc agtatcaggt
    28261 tgatggtcaa acaaaaacac gcaaagtcgt agatccacac tcatttcgca ttctgactaa
    28321 cgaagcaggc gaacctgagt atcaccgcgt acagctgttc acgacgggcg atgaacgccc
    28381 gggcctgatg aacttcccgt ttgaagggtt aacctcagga tcgaaatacg ggacagccgt
    28441 tggcatcatc atgtttatgc tggtgattgg cggcgcgttt ggcattgtga tgcgtacagg
    28501 aaccattgat aacggtatcc tggcgcttat tcgccatacc cgcgggaatg aaattctctt
    28561 tattcctgcg ctgtttattc tgttttcact tggcggcgcg gtatttggta tgggagaaga
    28621 ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc cggctgggct atgacagtat
    28681 taccaccgtc ctggtgactt atattgccac gcaaatcggt tttgccagtt cgtggatgaa
    28741 cccgttttgt gtggttgttg ctcaggggat tgccggcgtt ccggtgcttt ctggctccgg
    28801 gttgcgcatc gtggtgtggg ttatcgccac tctgattggc ctgatcttta ccatggtgta
    28861 cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg accgcttctt
    28921 tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc tttggtgact ggctggtatt
    28981 gattgtcctg acagccgtaa tggtctgggt gatttggggc gtgatcgtta atgcctggtt
    29041 tattccagaa attgccagcc agttcttcac catgggtctg gtgattggca tcatcggcgt
    29101 tgttttccgc cttaacggca tgacggttaa taccatggct tcatccttta ccgaaggggc
    29161 gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc aaagggattt tgctgctggt
    29221 tggtaatggt gaagcaggta atgccagcgt gttaaatact atcctcaaca gcattgccaa
    29281 tgccattagc ggtctggaca acgcggtcgc ggcctggttt atgttgctct tccaggctgt
    29341 atttaatttc ttcgtgacgt ccggttctgg tcaggcggca ttaactatgc cgttactggc
    29401 accgcttggc gatctggtcg gtgttaaccg tcaggttacc gtgctggcgt tccagtttgg
    29461 tgatggcttc agccacatca tttacccaac ctcagcttcg ttaatggcaa cgctcggtgt
    29521 gtgccgggtg gacttccgta actggctgaa ggtgggcgcg accctgcttg gactgctgtt
    29581 tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg ggctaccact aaaaatgtta
    29641 agagccgcaa ttgcggctct tttttcattc tgccgtttca gtctctacgg cttcattttt
    29701 ggcattgcgc ttcatccaca acgccagcgc tttcagcgag tctggagtga actcatcgca
    29761 gcgtgcggtg atttcttccg gcgtcagcca gcaaactcca ctgacttcat cttcctgtag
    29821 ggcgaaggga ccgtgagaga cgcaactgaa caacgcgccc cagacacggc aatttttatc
    29881 ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc
    29941 cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc tgaactacac cgcctgcggt
    30001 cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt
    30061 gcccatgcca tcatgcacga cgatgtaagt tgcacgatga cgcagacact gtgcccgcat
    30121 ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc
    30181 agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac
    30241 ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg
    30301 atcattgagt gcgattacac taagtacgtc attatccagc atgccataac ccgccggatt
    30361 accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc
    30421 tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggtag
    30481 attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac
    30541 ctgttgccac ggcgcggtta tcgggaaatg cagcatcatt tgatccactt cgctgtcgca
    30601 gttgccacgc acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc
    30661 tggcgcgtaa ccctccggta aagcattacg cgggccgtga ttcaacacgt cgccaaggat
    30721 caccagccac tgggcaccgc tttggacaaa caactccaca acacgttccg tcgccggtaa
    30781 cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg
    30841 taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa
    30901 taaaacgctg gacagacgct cgctgccact ggaatgtcgc ataatccacc aggcgttctg
    30961 gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa
    31021 tgcaccattc accaaataaa ttcggctgac ccagtgctaa caaatgttct gccatcgcga
    31081 acagtttctc tgcactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa
    31141 agacaacatc cgtcggacgc tcttcgcgga tgggcatcag atcgctgcgc agccaggcct
    31201 gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg
    31261 gtggcgcaaa tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat
    31321 catcgatttg taataacggc acacggcgtg tctgaccgta accttgccac gtcggctgca
    31381 aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttttcctgca
    31441 acgccaccca ggcggataac acataagggg agaaaaagtg ggcatctgac caaagcgtga
    31501 tagcgggttt actcattatg tcctcggcaa taaagggggc tggctatcaa actatagagc
    31561 ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg ccgcgctttc tatacttgtg
    31621 gtgttcagat gattaaacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc
    31681 caatggtcac aaaattacgc tgtttctcga agaagcagag cttgattatc gcttgattaa
    31741 ggtagacctg gggaaaggcg gtcagtttcg tccggaattt ttgcgcattt cgcccaacaa
    31801 caaaattccg gcaattgttg atcattctcc tgccgatggc ggcgaaccgt taagcctctt
    31861 tgagtctggt gccattttgt tgtatctggc tgagaaaaca ggactctttt tgagtcatga
    31921 aacgcgtgag cgcgccgcca cattacagtg gttattctgg caggtaggcg gactggggcc
    31981 gatgcttggg caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat
    32041 tgaacgttat caggttgaaa ctcagcgtct ttaccatgta ctaaacaagc ggctggaaaa
    32101 ctcgccctgg ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt
    32161 taatgcctgg actcgcctgc gaattgacct cgcaatgtat ccggcagtca agaactggca
    32221 tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg ctaaaagcac aactcggtga
    32281 tgagcgttcg gatagttaac agaaacaggt tctcgtgtat tatttcatcc taagtaaaac
    32341 aacggagaac ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgtttg
    32401 cgtacgttta tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat
    32461 gtgacgatcc actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg
    32521 aattatcgca ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg
    32581 ctggaaaaat taactcagga tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat
    32641 gctgaagtgg agatcgataa actgcacgcg ctgcgctacg ccgattcggt atccatgacc
    32701 ttaagctggc agcgttaatc gccatatcgg gaggctgcat gaacatagtg atcaccggag
    32761 ggacgggatt aattggtcgc catttgattc cgcgtttgct ggagctgggc catcaaatta
    32821 cggtagtgac gcgtaacccg cagaaagcca gttccgttct cggccctcgg gtgacactat
    32881 ggcaagggct tgccgatcaa agcaacctca acggcgttga tgcggtaatc aacctggccg
    32941 gagaaccgat tgctgataaa cgctggactc acaagcaaaa agagcgtctc tgccaaagcc
    33001 gctggaatat cacgcaaaaa ctggtcgatt tgattaatgc tagcgacacg ccgccgtcgg
    33061 tactcatttc cggctcggca acgggctatt atggcgactt aggtgaagtg gtggttaccg
    33121 aagaggaacc gccgcataac gaatttactc ataaactctg cgcccgctgg gaagagattg
    33181 cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct gcgaaccggc gtggtactgg
    33241 caccggacgg cggtattctc ggtaaaatgc tgccaccgtt tcgtcttggc ctgggcgggc
    33301 cgattggttc cggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggca
    33361 ttctctggct gctggataac gagctgcgcg ggccatttaa tatggtttcg ccctacccgg
    33421 tacgcaatga acaatttgcc catgcgctcg gtcatgcgct gcatcgcccg gctattttgc
    33481 gcgtccccac aaccgccatt cggctgttaa tgggcgaatc ttcagtactg gtattaggtg
    33541 gacaacgcgc gctgcctaaa cggctggaag aagcgggttt tgcgtttcgc tggtacgatt
    33601 tagaagaggc gctggcggat gtcgttcgct gatgtggttt acagcaaaca tccgccagtt
    33661 aactccctgt gttacaggat tagtggcttt gcgcgataag atcgtctggt gaaagtcggg
    33721 tcaccatcat aactaactct ctgtctaaac ctctatccag catctcctga gcaatacgca
    33781 gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg taatctgtca gcaatggtca
    33841 tcagtttctc cttttcttgt ggtgcgcgtt ctgttatctc accaataaat gcacggaaac
    33901 gctgggcatc ccctgtttgt aatacgtaat taaacagggc ttttagctgt ctgtcattag
    33961 tgttccctgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac
    34021 gaatatgttt ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat
    34081 ccggcaccac ggtaatatcc accaacggaa acgctgatga atatattttg cgggctatag
    34141 caggctcggc aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat
    34201 gataaaacag catcgggatc accaatggaa gctctttata gcccgcatca agatggtttt
    34261 gcattgccgc aatggaataa cgcatcatgc gaaaagccat taattcttcc ggcttacttt
    34321 ggtgctctat cactacataa atataaccca ctccctcctg cgttttcaca gaccacaaga
    34381 ggtcggaata atattgccgc aggtcttcat caataaaact gtttggttct agtttaagcg
    34441 tcgttaaatc acacagtttg cgcagcggcg cgggaagatg aatattaata aaattccgcg
    34501 cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg
    34561 atattgtcat ggcgttccgt cacccctcaa atttaagatg acgcgacaat aaccacgatt
    34621 aacacccaat gcagcaggca gtttttatct ttggacagcg aattcagaag tatttttagc
    34681 ggactgcaac ttttacattt cagcggaagc tgactcgcaa attccccttg aaaatactcc
    34741 ggcagcgtta gccaccggag aggaaattat ttcagcgatc ccttaaggaa ctgttgcaga
    34801 cgagggcttt gcgggttgcc aaataactgc tccggcgcgc cctcttcttc tattttcccc
    34861 tgatggagga aaatgacgtg agtagaaaca tggcgagcaa agcccatttc gtgagtcact
    34921 accaccatgg ttttcccctc ttctgccagt tgctgcataa tacgtaacac ttcgcctacc
    34981 agttcaggat cgagcgccga ggtaggttca tcaaacagta aaacttctgg ttccatcgcc
    35041 agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg aaagatgcac cggatatttc
    35101 ccctgcgcac gttcgtctat cccgactttc gccagatact tcaccgcccg ctcgcgcgct
    35161 tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca tgacgttttc caacaccgtc
    35221 atatggctcc agagattgaa gtgctggaat accatcgtca ggcgtgtgcg cagtaagcgc
    35281 agttgatttt tatcggcgac tttgagttga ccgtctttgt cgcgcaccag attgatcgtc
    35341 tggccattga ccacgatcga tccttcactc ggtttttcga ggaagttaat gcagcgcaga
    35401 aaggtacttt tccccgatcc cgacgatccg atgatgctta ttacatctcc ggcattcgct
    35461 tgcagtgaga cccctttcag cacttcgtgt tcgccatagc gtttgtgcaa atcgataacg
    35521 tttaatttat tctcggacat cgtattctca gtgcgttgaa gaaggtttca catgctgcaa
    35581 ccagcgtttt tccgctctgc gaaagaggct gatcaggaca taagagatga ttaaatagag
    35641 caccgcggca atgccgaagg cggtaaaagg ttgatacgtg gcggcgttaa tatcgcgggc
    35701 tattttcagc agatccggca cagtggccgt aaacgccagc gcagtagagt gcagcatcag
    35761 gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc gcagaaggca aaataatgca
    35821 gcgatacatt ttaaaggtcg agaagccata ggctctggcg gcttcaattt ccccatgcgg
    35881 aaccgaacgg attgccccag caaaaatctc ggtagtgtaa gcgcaggtgt taagcgtcag
    35941 cgccagcacg gtacagttca ggccactgcg gaagaaagcg ttaaggaatt cggttccctt
    36001 aacaatctca agcgtgtaca tgccggaata gaacaccagc aactgaacat acagcggcgt
    36061 accgcgaaaa atataggtaa ataaccagat tggaaactgg atgtatttat tactggagac
    36121 acgaccaatc gccagaaaca gcgccaggac tccgcctatc actaccgaca aaataagcag
    36181 ccacagagtg atcgccacac cagtaaagcg ataaccgtcg gtccacagca gcggtttcca
    36241 gtattcatgt aagatttcga tcacaggtca gccctcttca cacccacgga gtagcggcgc
    36301 tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa ccaggtaaat cacgccacag
    36361 acgatggcga aatagaacgg ttcccaggta cttttgcctg ccagctgcgt ggctttgacc
    36421 acatcttcca ggccgagtaa cgaaaccagt gcggtagatt tgaggatcac ctgccagttg
    36481 ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa acatgatccg ccgaaacact
    36541 tgcccgcgag taaaaccgaa cgccgtcgcc gcctctatat gccctttcgg cactgccata
    36601 aacgcgccac ggaacgtttc ggtaaaataa gcaccgtaga taaaaccgag agtgataata
    36661 ccagcgacca tcggatcgat atcaatctgc ccgacgccca tcgcctccgt caccatgttt
    36721 agcgcaatct gcaaaccata gaaaatcagc agcatcaaca ccaagtccgg cacgcctcga
    36781 atcagcgtgg tgtacccttc gaaaatcagc cccgaaagcc gattttgcga gagcttaccg
    36841 ccagcgccaa ttaaaccgat gattacagcg agcactacag agctgatagc cagctccagc
    36901 gtgacgagcg caccctgtaa aataacacct gaaaacccat acaacatgct gcctgtcctg
    36961 tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt acccgaaggg agcgggcttt
    37021 ctttcaccga tggcgattaa ccaccataaa catcaaaatc gaagtacttt ttcgctaatt
    37081 tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt gttcagtgcc tcgcgcagtt
    37141 cgttatcttc tttacgcagg cccatgccgg tccctacgcc aaacagtttt tcatctttaa
    37201 cagacggacc accgaatttg taatctttac cgacgggttg tttgaggaaa ccttcgctgg
    37261 cagcgacctc atcctggaac gcggcatcaa tacgtccggc agtcaggtca gaataaatgt
    37321 tgtcctgccc ctgatacgag acgatttcaa tgccttttgg tgcccagtgt tcattaccga
    37381 acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt gcctttcagc gactcgactg
    37441 tcggctgaat gtcagaattt ttcgccacca ccagacgaga atcggcagcg tacagtttgt
    37501 cggtgaaggc aatttcttgc tgacgttttt ccgtaatgga aagcgatgac atgatggcgt
    37561 caatcttctt cgcttttaag gacgggatta acgcatccag cgggttttcg acaaacgtac
    37621 attgcgtatt gatgcgcttg cacaactcct tcgccagctc gatatcgaaa ccaaccagtt
    37681 cgccttgtga attttttgat tcaaatggcg cataggtcgg gtcggtaccg atgcggatgt
    37741 tttgcggaat tgcagcaagt gccgcagttg cgctggagaa ggccagaacc agagagagcg
    37801 atagcaccag ttttttcata atgtcctcaa ctgacagtct ttttataggg aatatttaca
    37861 ggtttgttac acttatcgtg ccataaaatt gaccatcaag gcaaaatatc ccgccccgtt
    37921 agacgtatta tttttcatta aaaatgctta agtatgtatt tcaatgcacc acgatagtgc
    37981 aacaaacctg gcgtgcacca tgatgatcat tccatcaggt acagcttccc agcgacgtat
    38041 cagtcaccgt agacattaaa gtcgaaatac tttttcgcca tcttgtcgta ggtgccgtcc
    38101 tgacgcagct cgccaagcgc cttattgaag gcagccgtca gttcagcatc atctttacgt
    38161 agccctacac cggtgccatc accgaagtat tttttgtctt ttactgatga gccagcaaaa
    38221 gcgaaatctt taccagcagg ttgcttgagg aatccttcgc tggcagcaac ttcatcttgt
    38281 aacgcagcat ccagacgtcc tgcagccaga tcggaataga ccaaatcctg gttggcatag
    38341 gccaccacat ccacgccttt actacgccag gtctcgttag cgtaagcttc ctgggttgat
    38401 ccctgcagca caccaacatg tttacctttc agtgaatcca gcgttggctg aatcggtgaa
    38461 cctttggcag caatcaaacg agaatctgcg gcgtacagct tgtcggagaa ggcaatctcc
    38521 tgctgacgtt tatcggtaat ggaaagcgac gaaataatag cgtcgatttt tttcgctttc
    38581 agtgagggga tcagcgcgtc aaagtcactg gcaacccagg tacatttcac ctgcatccgt
    38641 ttgcacatct cgttaccgag atcgatatca aagccaacaa aatcaccttt agcatctttc
    38701 gacgagaaca gcgcataggt ggtatcggtt ccgatacgta ccgtctccgg tagcgccgca
    38761 tagctggaag ccgctgcgga gagaccgact aataaagaca gagcgagaat cgacttcttc
    38821 atacataacc ctcaagtgaa aatggctttt ttatgttgtg tattgtgttg tgtgtttgca
    38881 ggctctttca tgcaggtctt atgccatctt gacgacaaca gtaacattca acgttaaata
    38941 tgttaataag acgttgcatt attgtcctga agttgaagat agcaggtata gcggttgaat
    39001 cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg atttgatcgc ggttgcaaaa
    39061 ttgccctgaa acagggcaac agcggagtta tgcgccctgc cagcgggcaa agagatcttc
    39121 aggaagggtt attgcaaact ggtcaagaac acgattaacc gtctgattta tcacatcatc
    39181 aagggattgc gggcgatgat aaaacgccgg aacgggaggc ataatcaccg caccgatttc
    39241 tgccgcctga gtcattaaac gcagatggcc taagtgcaat ggtgtttcac gcacgcagag
    39301 caccaacggg cgacgctctt tcagcaccac atctgccgca cgggtcagta agccatcagt
    39361 atagctatgg acaatgccgg acagggtttt gattgaacag ggtaaaatca ccatccccag
    39421 cgtctggaaa gaaccggaag agatgctggc ggaaatatcg cgcgcatcga gcgtgacatc
    39481 agctaatgcc tgcacttcgc gcagggaaaa atccgtttcg agggataagg tctggcgcgc
    39541 cgcctggctc atcaccagat gcgtttcgat atctgtgaca tcgcgcagaa cccgtaataa
    39601 gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct acaatgagtc gtttcataaa
    39661 tagttgccct gtcagacttg caggcagact ttgcagtatt tcggcgggag ttgcaagtca
    39721 gggtgccaga ccggcaccct cagcgaaggc atcatccttc gttatgcatt tcgagatttt
    39781 ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt acgtaacgta tcgaggaaat
    39841 cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc gttgaacacc gagcattcaa
    39901 actgctggat atccggattt tcagcgcgaa cggcgtcgat cagatcgttc agatcctgga
    39961 aaatcaaccc gtcagcaccg atgatctggc gaatttcatc aacttcgcga ccgtgagcga
    40021 tcagttccgt ggcgctcggc atatcaatac cataaacgtt cgggaagcga atttccggtg
    40081 ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg tgccatctcg ataatctgct
    40141 cagaagtggt gccacggacg atggagtcgt cgaccagcag gacgttttta tcgcggaact
    40201 cggcgcggtt ggcattcagt ttacggcgca cggacttacg acgcagctgc tggcccggca
    40261 tgataaaggt gcggccaaca tagcggtttt taacgaagcc ctggcggtac ggtttgccca
    40321 gaatacgagc aatttccagc gcgatatcac acgaggtttc tgggatcggg atcaccacgt
    40381 cgatatccag atcttcccat tcgcgggcaa ttttctcgcc cagtttcgtg cccatattca
    40441 cacgcgcgct gtaaacggaa attttgtcga taaacgagtc cgggcgggca aagtatacat
    40501 actcaaacag gcacggattg ctgaccggat tgtcagcaca ttgacgggta aacaactgcc
    40561 cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc acgcaggaaa tcaaagccca
    40621 gcgtatcgag cgctacgctt tcggaagcga ccatatattc tgtacggttc tcgtcaatat
    40681 cacgttttcc cagtaccagc ggacgaatcc cgtttggatc gcggaaagca accataccgt
    40741 ggccgataat catcgccaca caggcatacg cgccgcggat taagcggttt gtggcagcaa
    40801 tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg gaagttgtcc agctcgctgg
    40861 cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt gatgtggcgg cgtttttctt
    40921 caaacagttt tttacgcaac tcgtgagcgt tggtcagatt gccgttgtgg gcaagcgtaa
    40981 tgccatacgg ggagttaacg taaaacggct gcgcttcaga ggcgctggag ctgccagccg
    41041 tagggtaacg cacatgacca atgcccatat tgccctgcaa acgctgcata tggcgagctt
    41101 caaatacatc gctcaccagc ccgttcgctt tacgcaaacg gaagcagtta ttggcatcta
    41161 tggtgatgat gccggcggca tcctgaccgc gatgctgaag caccgttaag gcatcataaa
    41221 tcgactggtt aaccggcata acaccggcga taccgacaat accgcacata cgtctttttc
    41281 ctcgttaagc cacatctcag agcacttacg ctctgggcaa gaaacttgac gagctttgca
    41341 gataatcaaa aaaccatctg atgataaaac tgaattgcgg gatcagctgt gatttgctcc
    41401 agtcttcact tttcgacacc ccggtaaagg agtcgagaaa gaagagaatg gcggcaacaa
    41461 tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac ccgatcggtg cctgacaacc
    41521 ccgttttctc caccaactgg cctatcacga agttcacgat agcaccaacg atcagggtag
    41581 cgataaacag taccgcgatg gcaatcccat ttcgaaccag ttcgtcttca aagcccgtaa
    41641 accagactga caggtaagtg tagtaatgac tggcaacaaa gaaagcacaa ccccatgtca
    41701 ccagcgataa cgcttcacga acaaagccgt ggatcaggct aaccagagag gaaaaagcaa
    41761 tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt cccacgattt tacgccctgt
    41821 catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt ttgcgtaggg atttccttcc
    41881 cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc catcgacttt ttatgccttt
    41941 gcggcatcgt gcaatgcgtg tcggatgcgg cgtaaacgcc ttatccgacc tacggttcta
    42001 acactgccca agcctgataa gacgcgccag cgtcgcatca ggcaagactg tattaattcg
    42061 gcgtatagcc cattaccacg ccacttaagc cagaaagttg cttcaactca cccagcgaac
    42121 ctttcagctt atctttcgag gcatccggcc caaccagaat acgggtaatt ttaccctgca
    42181 ctggcgtgga tggcgacgta taaacccgat aaccggcacc gcgcagctta ccgacaatct
    42241 cattcacttt atcggcattt ttcagcgcac ccagttgcac aacataggct ttacccgttg
    42301 gcgctgcttt ttcttccacg accggcttcg gttctggctt cggcgcaggt ggtgcttcaa
    42361 cctttggttt aggcggctcc accggtttcg gcttcggtgg ggcgaccggt gcaggttccg
    42421 gttcaaactc ggtgttattg gctgcaatag tggccggatc gagcgacggt gctgcggcat
    42481 cacctgcccg cacctcttcc gctgcgcctt ccggcggctg cgtcggcaac gcctgggtgg
    42541 cggctggcat catatcaggc tcatcacgat cgcccgcttt cggcaccagc gggatagccg
    42601 cgaactcatc ctgataatgt tttttctgcc cgtccagcag ccctggaagt acaatcaccc
    42661 ccagcgccac cagcacgatc gtgcccacta accgattctg aaacttactt gccaccgctt
    42721 ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt ggaaagaacc acacaccagc
    42781 acggtgtctt ccgctttagc gtccgccatt gcggcatccc atgcctgcgc aacgctatca
    42841 aatgatttgc cgttacccaa atgctcaagc agttgttctg ccgtggcacc gcgcggccct
    42901 tccagtggcg cacaatacca gtcatcaacc acgcttttca accaggccag agttccggca
    42961 atatctttat catgtagcat accgataacc gccagcacgc gcccgttttt cggtagcgct
    43021 ttcatacgcc cggtgagata ttccgccgca tgtggattat gcgcgacatc aaaaataacg
    43081 cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa ttgcgctggc aatcccgtcg
    43141 cgaatggcat tttcactgac ttccagcccg ctggcacgca gtgccgccag cgccgttgcg
    43201 gcattcggtt gcgggacaag cggcaacggc agattttcca gcgtgccgtg agcatcgcta
    43261 aacgcccagt catgatcggt gacggaatag ttccactcaa cgccccgacg ttgtaacagt
    43321 gcaccttttt cctgcgccac atcagcaatg gtagaaggca tttccggctc accgacaatt
    43381 gccggttttt cgctgcggaa gatgcctgct ttctcgcgac caatactttc gcgatctgga
    43441 cccagccagt cggtatgatc cagcgcaata ctggttacta ccgcgacatc ggcatcgaca
    43501 atattggttg cgtccagacg accgcccagc cctacttcca gaatcaccac gtcaagttgt
    43561 gcctgcttga acagccacaa cgccgacagc gtaccgtact cgaaataggt cagggaaata
    43621 tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt gggccgattc cggcaattcc
    43681 tggccctgca cacgtacgcg ctcggtataa cgcaccagat gaggcgaact gtagacgccc
    43741 actttgtacc ctgccgccat cagaatcgac tccagcgtac ggcaggtggt gcctttgcca
    43801 ttcgtacccg caacggtaaa cacaaatggc gctggtttca ggacgccaag acgcgccgcg
    43861 acctggctca cgcgctcaag gccgagatcg atagttttac tgtgcaggtt ttccagataa
    43921 gaaagccacg aagccagagg cgacgcggct tgaggagtgc gtttgataat catggtatcc
    43981 gctgattcgt tacggtgaga atagcaaaag ggcagagcca gtggccctgc ccttatcagt
    44041 tatcaggcct caggttcctg atccggtacc gggggtacca ctacgccttc acgcggcgct
    44101 tcaggattcg gcgctggcag attcatcaac ttcgccagaa tgctcgccag tttcaggcgc
    44161 atttccggac gacggacgat catgtcgatc gcgcctttct cgatcaggaa ttcactgcgc
    44221 tggaatctag gcggcagttt ttcgcgaacg gtctgttcga taacacgcgg accggcaaag
    44281 ccgattaacg ctttaggttc agcgatgttg agatcgccca gcatggcgaa gcttgcagaa
    44341 acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg gcaagccgcg ctcctgcatt
    44401 tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc ttcctgcata
    44461 cgtgcgccac cagaggcgga gaagcagatc agcgggcagt tatcttcctg cgcctgctca
    44521 acggcacgca cgaaacgtgc acccacaaca gaccccattg aaccgcccat aaaggcgaac
    44581 tcgaatgccg cagcgacaac cggcattcca tacagagtgc ctttcatcac caccagcgca
    44641 tctttttcgc cggtttcttt ctgcgcagat gccagacggt ctttatactt cttggagtca
    44701 cgaaacttca gctcatcttt cggctcaagc tcgctaccca gctccacaag gcttccttca
    44761 tctaacaggc tatgcaggcg attacgcgct gtcatacgca tgtgatggtc acacttcgga
    44821 cagacctcaa gattacgttc cagctcagcg cggtataaaa cctgaccgca gctatcacac
    44881 ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt gcttttaatt
    44941 cgttcaatcc agctcattag ggacctttct gtctgaacct ggttcgatgc cagttttatc
    45001 tttggggacg cataatgcca tttttgcccc caacagacca tgaatgttgc acattaaaac
    45061 ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg cggagttact ttttattttg
    45121 cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat gacgcccggc aaaatagaaa
    45181 ccacaataat cccgacgatc agcagcttaa ggttatcctg aaccatcggg attgtaccga
    45241 agaaatagcc tgcgtaggta aaaagcagta cccacaacag tgcgccgatc acgttataag
    45301 cagcgaaatg acggtacgac atgtggccca ttcccgcaac aaacggggcg aacgttctga
    45361 cgatcggcac aaaacgggcg agaataatcg ttttgccgcc atgtttctca taaaactgat
    45421 gggttttgtc gagataactg cgacggaaaa ttttcgaatt gggattactg aataactttt
    45481 caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc cacaatcgcg gcgatcagca
    45541 tcagcactac catcatatgg acgttgagat cgttggtttc cagtgatgcc aacgctccgg
    45601 caacaaacag caatgaatca cccggtaaaa acggcattac caccagaccg gtttcacaga
    45661 acaaaattaa gaacaaaatg gcataaaccc agacgccgta ctccgcgact agctctgcca
    45721 gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc atatttgcct
    45781 atgccttgta ctcgtcatct ttcaggctgt aactacgttg gctacagcct gaaatgttcc
    45841 gagcattatt ctcgttagtc cgccagaaat agcgggccca ttggcggttt tggaagatca
    45901 taccggtcag ggtaatccac cgcgaccaga tacagccctt ccgcttttgc cgttgctgcc
    45961 gccagcgttc tgtcctttgc cgccagcaac tctgctatcc agctctccgg ctggttgtgg
    46021 gcaccgactt ccatcaggct gccgacaata ttcctgacca tatgatgtac aaaggcattc
    46081 gctttgatat caaccaccac ataaggaccg tgacgcgtga cattaatatg cataacgttg
    46141 cgccacgggg ttcgggactg gcactgcacc gcacggaacg aggtgaaatc attctcgccc
    46201 agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta aaaatgggtt
    46261 acccctttac tcagtaccgc cgggcgcagc cgatgattgt agatgatgta gcgataacgg
    46321 cgagccgtgg cgctaaatcg tgcatgaaaa tcatcaggta cagctttaac ccaacgcacg
    46381 gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc acgccgcgtc tttgcgctgc
    46441 gcggttgttt cgaaatgcac cacttgcccg gtaccgtgta cccctgcgtc agtacgcccg
    46501 gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga gcgccttttc cagcttctcc
    46561 tgcacactgc ggacttcatt ctgccgttgc cagccgtaat acttactgcc gtcgtactca
    46621 atgcccagcg caattttata aactggcagt tgttgctggt cggacattag tacagatact
    46681 cctgcaccag tttctcggcg atttttactg ccatcagcgc gccgccaaag cgaacgttat
    46741 cggccaccga ccagaactgg acttgctccg gcataccgta gtcattacgc acgcagccaa
    46801 cagaaagatg cggcgtaccc gaagcatccc ctacctgagt cgggaattcg ttctcttcag
    46861 agagcacaat atcttcgccc tgagcaaacg catcacgcgc ttcttctgcc gccagcggac
    46921 gcagtgcttc aaagttgacc atctgggcat gaccgtagaa taccggtgcc tggacgacgc
    46981 ttgccgaaat catcagccct tcgtcctgca ggattttgcg tacttcgtca acgatacgac
    47041 gttcttcacg cacgctacct tcgctatccg gcaacaacgg cagcatattg aacgccagtt
    47101 gacgaccgaa gaaatcttct tcgtcaatcg gaatgccgtt gagcaatttc gcactctgcc
    47161 ccgctaacgc atcgaccgct tttttgccct gggcggaggc tgaaatcagg ctggtaacac
    47221 tgatacgcga caaaccgccc tgatcgatta acggtttcag tgccgccagc agctggctgg
    47281 tcagactgtc tggtacggcg atgacattcc ggttgcggta atcggtcagt acaaacgggt
    47341 ttacttccgg caccaccagc ggtacgtcgg gttcgagagc aaacagtccg ctgctgtcga
    47401 tcaccaggca acctgagttg gtcgcttctt caacccaggc agcggtagct tctttgcctg
    47461 cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc agcggcatcc tgcacggtga
    47521 ttgttttacc accaaagcgc agttgttcgc ctgcgctttc gttacgtgcc agtgcataaa
    47581 tttccccaac cgggaactga cgttcagcca gcgtttcaag cagggcttcg cccatagcgc
    47641 cagttgcgcc caggacggca atgttccagc cttcagacat ggtggtttac tccagaaata
    47701 gcaaagctcc ctgccaatgt tacagcagag agcatgaaga agagattaac gtgccggatg
    47761 atgaacggcg ttaaaaccca gtttacacag caatgatgcc gcactggcgt catcacaaat
    47821 tacatacaga gatgaccatt cacggcgctc aagatagttt ttgcgcagtt tatcgaactc
    47881 acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg cggcgcacat catacaccaa
    47941 atgcaccagc cttttcagcg tcggttgatc gagcggacca tgcagcgtaa tgcggccaaa
    48001 ctcaggcgcg ggcagcaacg tatccagcgc aacgtgttgt tcatgcccaa taaacttgct
    48061 ataggcttca aacacttgcg tcgtgccacg cgctttaccc tccagggtat agcctgcgat
    48121 atgcggcgtg ccgatatcca cttttgtcag caattccaca ttaagttccg gctcgccttc
    48181 ccagacatcc agcaccacgc ttaacttctg gccttcattc aggcaggtca gcagcgcggt
    48241 attatcgacg actgcgccac ggcaggcgtt aatcagaatc gctccgggct tcaggctacg
    48301 gatcagtttt tcatccgcca gatgtagcgt tttgtacgga ccatctttaa agagtggcgt
    48361 atggaaagtc agaatatccg cgcgctggac taactcatcc agcgagcgga aatcaccctc
    48421 atccccacgg tcggcgcgag gcggatcgca aagtaaggtt ttaatcccta acgcttccag
    48481 tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg atccccaccg tacggtcgtg
    48541 cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag gagaaaacat attccaccac
    48601 cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt cccgcctgct tcaaccatgc
    48661 ttcatcgaca tggtcggtcc ccgcagtggc agttccaaca aatttaatgg gttttcctgc
    48721 cagcaaagat tcattcactt tcgtgaccga acgcaccatc agcgcatccg cgtctgccag
    48781 ttgagcgacg gggattggac gcccgggaac cgcgatcacc tcacccaaac ggctaaataa
    48841 gtcgcgggca taaggcatat tttcatcaac aaggattttc acgtttgtgt tacctgtatg
    48901 agacgagagt taaccggaca agtatgccat aatctcgcgg ccaggcatac ttgcgaagat
    48961 ttcaggtata aggatacgta atgatacaac ctatttccgg ccctcctcct gggcagccac
    49021 caggtcaggg agataacctg ccgtctggcg cgggcaatca gcctttatcc agtcagcaac
    49081 gtacttcgct ggaaagctta atgacgaaag tgacctcact gacgcaacaa caaagagcag
    49141 aactgtgggc gggtatcagg cacgatattg gtctgtcggg agattcaccg ctgctttcgc
    49201 gtcacttccc tgccgctgag cataatctgg cgcaacgtct gctggccgcg caaaaaagcc
    49261 attctgcccg ccagctttta gcgcaattag gggagtattt acgtctgggg aatcatcgtc
    49321 aggcggtcac ggattatatc cgtcataact ttggtcagac gccgctgaat cagctctcac
    49381 cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg gaagatggtt attccgcaac
    49441 cacagcagcg cgaggcgacc gaccgtcctt tattaccggc ggagcacaat gcgctaaaac
    49501 agctggtgac caaacttgcg gcggcaacgg gggaacccag caaacagatc tggcaatcga
    49561 tgctggaact ttccggggtg aaagatggcg agttaattcc agcgaaactg tttaaccatc
    49621 tggtgacctg gctacaggcg cgtcagacgc taagccagca aaatacgccg acgctggaat
    49681 cactacagat ggcgctaaaa caacctttag atgccagtga actggcggcg ttatcggcat
    49741 atatccagca aaaatatggc ctttctgcgc aatcatcgct ttcttctgcc caggccgagg
    49801 atattcttaa tcagctttat caacggcggg ttaaagggat tgatccgcgt gatatgcaac
    49861 cgctgcttaa tccttttcca ccgatgatgg acacgttgca aaatatggca acgcgtcccg
    49921 cgctgtggat actgttagtc gcgattatcc tgatgctggt ctggctggtt cgttaacccc
    49981 gacgaaatga cagtatcgtg acaataatac ccagcaccgc agagatcgcc ccggcaagaa
    50041 ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc cagcggcccg gagacgccga
    50101 gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaact tgtgagggga
    50161 cgcgtttaac cacctccacg cccagcgcag gaaagataag cgaacatccg gctccggtta
    50221 acgccgcgcc cgctaatgcg acccatgcac ctggggcttg ccagagcagc aacaagccca
    50281 ccgtttctac aagcagagag acaatcgcca ctttcacgcc gccaaaacgg tccggcatcc
    50341 agccaaacat gacgcgcatc acgacaaatg cgccgccaaa cgcggtaaga gtaaagcccg
    50401 ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa agtcccgata accgcaaaac
    50461 caacgccttg tagtgccagc cctaaccctg gtttccagat aagcccgaca acgctccaca
    50521 gcgatggacg ttctcccgcc agggccggta ctttgcgcac tgtgccgtta caggcccacg
    50581 ccagtaaggg taataccatt gtggtgatcg ccagtgcggc aaaaccgtaa tggctatgaa
    50641 tcaacaggcc aagcggcgca ccaacagcga gggcaccgta aatcgccatt ccgttccatg
    50701 acatcacttt gccagagtgt tttggcccta cgatgcctaa cccccaggtc agagcgcctg
    50761 tcagtaactg gctttcacca aacccaagaa tcaaacgccc gatgaccaac agggcaaatt
    50821 tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa cgcgccgcca gccagaccac
    50881 aagctaacat cccctgaagc gccgaacgtt ttgcgccata ttgatcggcc agtcgcccgg
    50941 cgtaaccacg cgtcagcacc gtagccagaa actgaatccc gacggcaatg ccgaccatgg
    51001 tattgccata gcccagatca tgatgaacaa acagcgggat aaccggcaac ggcaacccta
    51061 cggtcatgta ggtgagaaaa accgcaaaag agatgcggaa gagcgaaaaa ctggcagaag
    51121 atcgtgtttc ggtttggctt acagcagtca tgcattactc cagaatgcag cgcaaggcga
    51181 ggagtatccc cgtctcatct ctctggtttc agggttacgg tgcgttggca ggatttaacg
    51241 cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt tgcctagaac tggtggcgat
    51301 tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt gcgttagtgc cggaggccgc
    51361 attggcgcgt aacgtcggat gcgacgctgg cgcgtcttat ccgacctact ccggcctact
    51421 gcgaattaat cttttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa
    51481 gctgttagac ataacagtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc
    51541 cgcagcctgc tcgtccagct cttcaatgtt gatgctcggg gcgataaagc cgtgttccag
    51601 catcagcaga gagtagatag cttcctgcac acccgcagcg cccagtgagt gaccggtcat
    51661 ggctttggtt gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag
    51721 ctctttcacg tcgccaaccg gagtcgaagt accgtgagag ttcaggtaat cgattggggt
    51781 atcaacgcca tgcatcgcca tctgcataca acgtactgcg ccttcgccag acggagcaac
    51841 catgtctgca ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtaagcacc
    51901 acgcgccagc gcgtgttcca gctcttcaac cactaccata ccgccgccgc cagcgataac
    51961 gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt
    52021 agtagatagc gcacccatcg cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc
    52081 gccgccagca aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat
    52141 acagtgtgcg gaagtcgcac acgcggagct gatggagtag ttaacgccat gaattttaaa
    52201 cggcgtggcg agacaggcag aaacgccgga tgccatcgct ttagtgacca catacgggcc
    52261 aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg ccgaacacct ggaaacgcgg
    52321 ggaaccgccg ccggaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg
    52381 agagaggccc gcatcagcga ttgcctgctc catagaaagg aatgcataaa tggatgcgtc
    52441 gctcataaag cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttatgttgcc
    52501 ccagacgtgg ctacgcatgc cggaatcctt cagctcctga gagaaagtga tccctgaacg
    52561 tccttcacgc agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc
    52621 caggccagta atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt
    52681 ttcgaatgca caatagcgta cacttgtacg ccgaacaagt ccgatcagcc atttaataga
    52741 gatatttgcg cagccttaca cacatcgcta agatcgagcc accgcctgta agacgagtaa
    52801 cttacgtgaa acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac
    52861 ctgtttcccg agattttgac gatgtctatt tttccaacga taacgggctg gaagagacgc
    52921 gttatgtttt tctgggaggt aaccaattag aggtacgctt tcctgagcat ccacatcctc
    52981 tgtttgtgat agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggaagg
    53041 catttgatca gtttcgcgaa gcgcatccgc aagcgcaatt acaacgctta catttcatta
    53101 gttttgagaa atttcccctc acccgtgcgg atttagcctt agcgcatcaa cactggccgg
    53161 aactggctcc gtgggcagaa caacttcagg cgcagtggcc aatgcccttg cccggttgcc
    53221 atcgtttatt gctcgatgaa ggccgcgtga cgctggattt atggtttggc gatattaacg
    53281 aactgaccag ccaactggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg
    53341 gctttgcgcc agctaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa
    53401 ggttggcgcg tccaggcggc acgctggcga catttacgtc tgccggtttt gtccgccgcg
    53461 gtttgcagga agccggattc acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa
    53521 tgctttgtgg ggtgatggaa cagacattac cactcccctg ctccacgccg tggtttaatc
    53581 gtacgggcag cagcaaacgg gaagtagcga ttatcggcgg tggtattgcc agcgcgttgt
    53641 tgtcgctggc gttattacgg cgcggctggc aggtagcgct ttattgcgcg gatgaagccc
    53701 ccgcgctggg tgcttccggc aatcgccagg gggcgctgta tccgttatta agcaaacacg
    53761 atgaggcgct aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc
    53821 aattacccgt taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga
    53881 aaagccagca taaaatcgca cagatgttgt caatggattt acccgcagaa ctggctgtag
    53941 ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggcatta
    54001 cttatccgca aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg
    54061 cgcaacagca gggtttgcag atttattatc aatatcagtt acagaattta tcccgtaagg
    54121 atgactgttg gttgttgaat tttgcaggag atcagcaagc aacacacagc gtagtggtac
    54181 tggcgaacgg gcatcaaatc agccgtttca gccaaacgtc gactctcccg gtgtattcgg
    54241 ttgccgggca ggtcagccat attccgacaa cgccggaact ggcagagctg aagcaggtgc
    54301 tgtgctatga cggttatctc acgccacaaa atccggcgaa tcaacatcat tgtattggtg
    54361 ccagttatca tcgcggcagc gaagatacgg cgtacagtga ggacgatcag cagcagaatc
    54421 gccagcggtt gattgattgt ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg
    54481 ataaagaggc gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca atggtaggca
    54541 atgttcccga ttatgaggca acactcgtgg aatatgcgtc gttggcggag caaaaagatg
    54601 aggcggtaag cgcgccggtt tttgacgatc tctttatgtt tgcggcttta ggttctcgcg
    54661 gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc gcagatgagc gacgaaccga
    54721 ttccgatgga tgccagtacg ctggcggcgt taaacccgaa tcggttatgg gtgcggaaat
    54781 tgttgaaggg taaagcggtt aaggcggggt aatctgctct cgcagtgttt gccggatgcg
    54841 gcgtgaacgc cttatccaac ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt
    54901 cgcatcaggc atggtgctcc agacgccgca acttacagct gtgacgcctg ctgaaacagg
    54961 ttttcccaca tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg
    55021 gctttttcca gactctggct aacggtcgta tgcaccgcct gagcggagtg gtcatcacca
    55081 ctttccagtt ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac
    55141 tcatcatcac tggcatggtc aaccatgccg tcgattaatg ccagaatgcg tgattcaaac
    55201 tccgcgatca tcttctttcc tcattgtaaa acgtggcgct gacttcagtt gagcgcttcc
    55261 ggccacggga actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta
    55321 atgaagcgtg ccgggcgttc cggaatgcca tttgtcagat aatccatcac ctgcgcatgt
    55381 acccgccgtt gaaacaccac acgatccggt tcgaaatccc cttccagatt gtcgcaactg
    55441 acgttaaacg gatatccagc cgccacgcag aataaccagt cgaacgcctg cggcttcact
    55501 tcaacatctt caaactggct ttgcgtctgg gcatcgcgtc cgtccgggca ataccagtag
    55561 ccgaaatcaa ccagctcacg gcgcgctttt ccggcaatac accagtgcga aatctcatga
    55621 attgcgctgg cataaaagcc atgagcaaag acgattcggt tatacggtac ttccgcatca
    55681 gcaggaagat agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca
    55741 aagcagctat taaaaatttc aatcaactgc tcgtagtggt gtgtactgtt cattagttca
    55801 tccccaacca gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca
    55861 ccgccgagac gataacaatc atcgggcgga tcagcttttg tcctttgctc aacaccaacc
    55921 gtgaacccat gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa
    55981 tcactttgcc gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttgagta
    56041 atttggcgtg agccgtggct ttggcgaggt tgaatccgca cagcataacg aaggccagcg
    56101 cgtaaaacga tccggcagcc gggccaaaga atccgtcata aaaaccgacg cagccaccgg
    56161 caattaacgc gaagggtaaa ccgtacatcc ggcgctggcg atcttcttca cccagctttg
    56221 gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat gggcaaaatc tgccgtaaga
    56281 catcagcctg aacgtactgt accaacagtg cgccgctcat tgagccgaca aaggtcatgg
    56341 caatattgag tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtgg
    56401 cggaaataga gccgccgcag gcttgcagtt tattggttgc cagcgcgtta gcgggagaca
    56461 ttcccgcagc catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga
    56521 taaatcccgc cagcatggcg acaaaaaaga gaactcccag caacagcgga gaaaccataa
    56581 acaggctatt aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg
    56641 cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt cccggttttg gaggttcaaa
    56701 ccagctttgc agttctgccc cgcaaccatc gcctgatggc ggtaaaggtt gatcttcaca
    56761 ctccagacta tcggcagggc aacgtaatcg tacatgcata tgcgcgcgat gctggaacca
    56821 gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt
    56881 aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa
    56941 gctgaaaatt tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa
    57001 gtccagtgct tgcgggcgca agagctgcgc ggaggtccag cgagttttcg gcagttgcag
    57061 aaagatatcg acatccagtc cggtctggtg gctggcatga ccgccgttga aacgcccacc
    57121 agcgggcatc cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggct
    57181 actcagacgc tggataaaca tcaccagatc cgggtgaccg aaatagcgac gctgatcggt
    57241 acgcatgacc tgataatgtt cggactgtat cggcaacgtg tcagcgccga caatacagcc
    57301 attagaaaaa ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca
    57361 cggcgtcgct gccaggctag cgctactggc aagcagagcc agcagcgcaa tcgcggtttt
    57421 gttcattttt taccagcgtg gaatatcagt cttcacatcg gcattttgcg cccgctgccg
    57481 taacaggtga tccattaaaa cgatcgccag catcgcttca gcgatcggca ctgcacggat
    57541 cccgacacag ggatcgtgac ggcctttggt gatcatctca acttcttcgc caaagcggtt
    57601 aatagtgcgc cccggcacgg taatgctgga ggtcggtttc agcgccatat gagcaatgat
    57661 ttgctgcccg ctgctgatac cgccgaaaat gccgcccgca tggttgctct ggaaaccgtc
    57721 tttggtgatt tcgtcgcggt tctggctgcc gcgcagcgcc actacgtcaa aaccgtcgcc
    57781 aatttccacg cctttcaccg cgttgatgct catcagcgca tgggcgatgt cggcatccag
    57841 gcggtcaaag accggctcgc caagtccggc ggggacacca ctggcaacaa cggtgacttt
    57901 cgcgccgatg gagtcgccct cttttttcag cgcgcgcatc agttcatcta acgcgtcgat
    57961 tttgtcgggg tccgggcaga agaacggatt ttgttcaacc agcgaccagt ctttgatttc
    58021 cagcggaatg tcgcccatct gggtcaggca gccgcggatt tcaataccaa atttctcggc
    58081 gagatatttt ttggcaatcg cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga
    58141 acgtccaccg ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg
    58201 gcctggacgg aagacgtctt taattgcgct gtaatcctga gaacgctggt cggtattttc
    58261 gatcaacaag ccaatgctgg tgccagtagt aacgccttca aaaacaccgg agagtatttt
    58321 gacctgatcc ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacgatc
    58381 aaggtcatgt tgcaggtccg cttccgtcag cggaatgcct ggcggaacac catcgacgat
    58441 gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat
    58501 tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat
    58561 aaatcgcgaa atgttctcgt gcggcaataa gctgctcttt agtgagcata aacacaccat
    58621 cgccgccgtt atcaaactcc agccaggtga acggaacatc gggatattgt tccataagat
    58681 gtaccatgct gttgcccact tcacaaatca acacgccatc atcagcaagg taatctgccg
    58741 cgttaccgag aatgcgacgc gtcagtttca ggccgtcggt gccagatgcc aggcccagtt
    58801 ccggctcgtg acggtattcg tttggcaggt cggacatatc ttccgcgtcg acatacggcg
    58861 ggttagtgac aatcagatcg tattgcactt tcggcaaatc acggaacaga tcggaacgaa
    58921 tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt ctgttcggca accgccagcg
    58981 cgtcgggaga gatatctacc gcgtcgactt ccgcttccgg gaaggcataa gcacaggcaa
    59041 tggcgatgca gccgctaccg gtacacatat ctaaaatatg ctgcggttgc ttgctgatga
    59101 gtccggcaaa tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt
    59161 catcgacgta aaattcatga ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa
    59221 tgcgctcgtt gacgcggcgg atcacgcgtt caacaatacg gtgtttttcg ctggaggtca
    59281 gacgcgcggt gcgcatatct tccgggatat ccagcggcag gtagagcgat ggtaacacca
    59341 gctgtacggc ttcatcccac gggttatcgg tgccgtgacc gtaccagata tttgccgcgc
    59401 tgaagcggct taccgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt
    59461 catcaacgaa aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc
    59521 tagtttgcca tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac
    59581 gtttaaaacg atcgattgtg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga
    59641 cagataaatg aaaaagaaaa caacacttag cgaggaggac caggctctgt ttcgccagtt
    59701 gatggcgggg actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa
    59761 aatcagcgaa gtgccggtga aacgcttgat ccaggagcag gctgatgcca gccattattt
    59821 ctccgatgag tttcagccgt tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga
    59881 tgtcagccat tttgaggcga agaaactgcg ccgcggcgat tattcaccgg aattgttttt
    59941 ggatttacac ggtctgacgc agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc
    60001 ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat gggcacggga agcatatttt
    60061 gaagcaacaa acaccgctgt ggctggcaca acatccgcat gtgatggcat ttcaccaggc
    60121 accgaaagag tacggcggtg atgcggcttt gttggtgttg attgaagtgg aagaatggtt
    60181 gccgccggag ttgccctgaa tgctggcagc aaatgccgga gcagcgtaaa cgccttatcc
    60241 ggcctacaaa tatgtgataa ttcaataaat tgcaattcac ttcgtaggcc tgataagacg
    60301 catcagagca atactacgaa cttcagttca ttcaatacat actgaaaacg acacgtcgaa
    60361 tcaatcggtt gcccactact ggtattcaca ttcaccacaa tatcgtgtag tactttatcc
    60421 gttaccggat gacgagttgg cgtaaccgtt accgttgatt tatcacaacc gtaagaggta
    60481 aaggttaaca attctgatac cgcgcggcag tatgctcctc tgccatcatt atcagaaaaa
    60541 ttactgccat cacagagtct gccgccttcc atcaggtagg tcgaaagcgt ggtggtcact
    60601 gttgaccccg tggactgaat tcgtaacgcc ttattatggc tgacataatt ttcactccag
    60661 gagatatcgt atgaagcgac cggtatcgtt gggcatattg tgttatgcac tgtaggggcc
    60721 gtatagcttc caaatccggg taaatctgta taggaataaa aggatgcgat ccaagggcaa
    60781 ccataatcat cagatgcaac ggtaatttta aacgtcatat atgcacaacg aaaaattttc
    60841 gtatctatgc cagggcaatt tgagttatta attgtatcaa agtcagtcag taccaaagtt
    60901 ggagctgaac ccgacccaga ccaggaaata acccgcaacg tatcgccttt atacgcaaaa
    60961 gaatcatatt tcccatatgc aacctccttt ggcgttatca ccgtaacatt gaaaacactg
    61021 ctcgctacag caccggttac cttgtccatg gcataataaa ctctcacact gtcaaaactc
    61081 gcactcataa gtttatctcc agcataactc ttagatgcgc cgacaacact cagaacgagc
    61141 agcattaggc tcgcgaaaat tatggtccat tttttcattt cagaaatact caaaggttgc
    61201 cgtcactgtg gcactaaagt ctccggacgt tacatctcgc ccgctctttg cctgtaacca
    61261 ggtattaaaa tttaggctgt tatttccctg attcagcggg agcgtgacgc ctgttgtatt
    61321 gttgattggc accgatgtgc ctgccgcagt ttcaatacca atcccagcgc cggatgctga
    61381 tgacgaggag tcaaaggcca gaaagccggg tagagcacta tcttctgttc ctgtcaacgt
    61441 tactttgaca ttaaacaatg tagaactatg acaatctttc aattgaaaca ccaatggcac
    61501 tcgctctgac tgcccaaggt tcattaaatc actggcggca atggcaggaa aatgtacttc
    61561 tgccaactgg ctaccattaa tcactggcgt acacgattta ctgattaaat tgccggtaaa
    61621 gtgcaaatta tctcctgcta ttgtggttcc actgcccagc aacatcaggc tactaataaa
    61681 aaacaacgta cgctttttct tcattggtaa tctacctgta acgtggccca ggcctcaaag
    61741 tccgcctctg gcagaatgac accactcttt tttactggca cagccgttaa caccggtaaa
    61801 tcggtttcat tcaccacaag tggcgtattg atcgtaaaac tctgccctgc ctgtttgagc
    61861 tgaatgccta aaccagtgat attgctgctt actgcagcat tatcaaaatc gcttggtgtg
    61921 ccactccagc tcaaggtcat ttccattaat gggtcgcgaa ccgccgtatc gcaggtaatg
    61981 gaatacggca cggtttgctc ataattgctg ccattaatgt catcaataag cacatcctga
    62041 aaagttattt caatggtttg agcgttgcta atcgtgcagt taggcggctg cactaaataa
    62101 ccgtgaaaag agacgtttga gagtgccatc gagcaaaagc cccacagcaa aataagcgag
    62161 atccgtttca ttggtaatcc accacaattg tggcactggc gttaaattca gctgccgcta
    62221 acgttgtacc actctgtttc accggcacag cttccagaac gggaactccc gaggaaaaat
    62281 taaatggcaa ccatgagcca gaggttaagt cgagtattgt gtgatcacta cttttctgaa
    62341 cccgaatacc aaacccagga atacccgtgc tgataactgt ttcaccgtta atcacgaccg
    62401 ttgtcgcctg catctgtaga cgcaggtcat ccattgccag attgttacaa acaagcgagt
    62461 agtcaatggg acgtttataa tcaaccctat tgatcttatt aatgaataca ttaccaaact
    62521 caactgccgc accatttacc gtacaaggag gaggggcatc gacaagcacc cgtaaattca
    62581 tacttactga cttactcgct gcaactgcaa agccgctacc aacacaacat atcagtaacc
    62641 cagttaccgc ccagacagaa caagatcctt tcatactggc tctcccggcc tcagcctttg
    62701 cggctctgct cttcatcgac ctagcaactg ttaccgttgc aagtgaaaaa gagcggcatc
    62761 cgagcgccat agtcgttgac ataggtcagt accggcacgc tacccatctt cacattcagc
    62821 ggtgcagagg ttttcggcga catcactatt agcgagaaac cggctgccgg attgccattt
    62881 ttctgtgtgc tggcattact gaaaatgacg taatacgacg tcgggttatt gacggtaaat
    62941 tcctgaccgt tgcgggttaa agtaatctta tattgctagg ggtttttcat gctgacgttc
    63001 tccagcgcct tcggtcgcca gaacaactta atacgcgttt gtagtgctat ttgcagcgtg
    63061 ttggctttgt ttgattttgg cgggatctcg cgcacgttga agtagaacag gctttcacga
    63121 tcggcaggca atttgttgat atccggcatt ccctgcactt ttacctgccc gttcatcatt
    63181 gaatcgatgc gttgcaccgg tggcagaaca gttaacggtg aactgatctt gttacccttt
    63241 tcatcttcga tccagctttg ggccagataa ggcagtttcg gatcgttatt gcgcaacgtc
    63301 acgctgattg atttgtcgct ttcattaaat atcaaacggg tacgatctgg cgttacagaa
    63361 gcctgggcag acaaactggc agcgctaagc agtaacgcta gcgtcgtagc acccaatttg
    63421 gcagagcaaa gtaaatcaga catggatcat tccttaatca cgttattggt tagctgaaac
    63481 agaaacaggg ggattggtgg gtgtcacctg ttgcgtctgc tcctgaatga caggctttat
    63541 gtcatcagga acaaccggcg cgacgttgcc tttatgctgg catggcagta acaagccgtt
    63601 aaacagatcg gcaggcagcg ggtccggcag gttgatatcg caatgcgcaa caccactcca
    63661 gaacacactc atgtgttcgc caggtttcac cccagccaga taaacattgc cgtcatcatc
    63721 gacaaggccc actgtctgct cgttatcatt ttttacttct gcgccaaacg gaggatggct
    63781 gccgtcttgt aaacgcagca ccgccatcgc tttttgacca ctgatgacgg caaatttgcg
    63841 gtagccaatc gccccttcag ttagcgtggc ttgtaccacc gactgggttg cttcggcgtt
    63901 ttccggcagt ttgttgaggt cgatatacgc ctgattgcgg taatagttat tgacgtcaga
    63961 aacgacggct ttaccaaaca tattggtata aacagcagca ccgttacctt ctaccggaac
    64021 atcggcaacg ccatcggcat caatcaacaa gcgtgtcccg cccatattct gggtacggtg
    64081 aagtgcgcca ccgtgggcag taagcgttgc gccgccctgt aacgacaagc ccgcagaggt
    64141 gtattgccct tcatggtagt tcgcactgag gtcaacctgc gccagcgaac catcatggct
    64201 gtaatagccg tcaacgctgg tgtgtttgtc actggtgccg acgttcaact gatagtgagt
    64261 cgcgtcatcg acacggctga aataaccgac ctgactgctg tccgtcccac tgccatagta
    64321 ccgttatagc taacggtact gttgtcgccc cacggcatac tgagcgaaat gtacatgcct
    64381 ttgtcggcct ggttgtcata ctcgtaacgg tagccagtca tcgagatgct gacattacga
    64441 atactgccca tattgaaata gtgcgagagc atgatgttgt agtttgtctg ctcctcgcga
    64501 tcccagtagg tatggcgggt atagttgaga taaaccgaaa cacccgcatc gcggaagttc
    64561 tggttataag tggcggtgta catctctttg tcgttgcccg tgcgtaccat tccgctgtca
    64621 ctggcatcca gatactcgct catggtcata aagttctctt ccgagaagcg atatccagca
    64681 aaagtaacgc ggctgttgag ctggtcaaag tctttggaat aactcacacg gaaggagtta
    64741 ccgtccagcg aacctttgcc ataagcggtg tctttatcca gtttggtgtg cgagtgagta
    64801 acatcaaacg cgaccgcgcc gaatgtagac aaatcgcgac cgacaccaag cgccgcagac
    64861 tgatagtttt catctcccag tgcgccgcca tataacgacc agccgttagc gatcccccag
    64921 gaagcttcag caccagaaaa aaattcaccc tcgacatggt gtccccactc ttgcggacgg
    64981 cccatcatga tcttatagcg aacctgacct ggacgagtga ggtatggcat cgaggcggtg
    65041 ctgatgtcat attcctgcac ctggccgttc tgttcttcaa tgcgaatatg caacgtaccg
    65101 gagacagaat caccaagatc ctgaatacga aacggtccag ccggaacctg cgtttcgtaa
    65161 atcacacgcc ccatctggct gacggtcact tttgctgtgg tgtgtgccac gccggaaatg
    65221 tctggcgcgt agccgcgcag attgggaggc aacatttgat cgtcagtact gacactgcca
    65281 ccaacatagt taaaaccatc aaaaatatcg gatctgaggt aatcctcgcc cagcgccagt
    65341 ttggctttta atgatggtaa cgcccgcctg gcatagtagc gactccactc ccattttttt
    65401 tgagtttcat cgccgctgaa ttcatcgtca tcattactgc gagtatgttg atagttagtc
    65461 tgccagtcag cacgcatacg ccacggccct aggttaaccc caaccgtccc gttgccgctg
    65521 atctcgttac tatcatcacc gccattttct tcgtgccggg tttgtgcgtt gatgctgtag
    65581 tccgcgacga tcccggagat gccgtcatcc caacgtgaag gcggatccca gtcgggataa
    65641 gtatattcga ggtaagcctg tggcagtgaa atgactaatg cggactggct taaatcagcc
    65701 ttaatttcca tgccttccag ttgaccggat ttcaggcatt tagcatcgtg gctccattgc
    65761 agatttttcg ccacgtcttc ttttaaacca aactgcgcta ccagttccgg tgtcagacaa
    65821 gcatagcttt tgctcgcgtc atcttcacca gcataccagt aaatatcgta ctcttccgcc
    65881 aatggctgtt tatttagttg aacctgtaaa ttgtattttc cgggctcaac atatccctag
    65941 ctggaaaaac gcttcagatc aatttttgtg tcgcctttta attccagaaa acgggaatca
    66001 aactgaatgt cgtcttcagc ccagacactg ctataactcc ctgacattgc cagcgcaatg
    66061 caccacggaa gaatccgtaa tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt
    66121 ttttattctt gttcggtcaa aatacattta aatcccccat agcagtgtcg ctatgggggt
    66181 atgccttaca ggtaagtaat ctggaaggtg gtatttgctt caaaattacc cagatctggc
    66241 gcatcagctg cgccgaccag ccaggcgtta aagttcagtg tctgcttcgc tttgcctttg
    66301 ttggtagaag tgtctttcac tattttctgg tcaataccca tgccgctttt gtaagaggta
    66361 cccagagagt caccgatcgc caggctaaca ttgttaaatg ccgcaccggt atcggtattg
    66421 aaaatggtgt aataattgcc gctatttgca gaagaaacgg taccagtaaa ggtagtggtc
    66481 attgtttcct gagtatcaaa tacgcaatct tgcagacgaa tctggaaact tttcggcgtg
    66541 gtggtgccgt tatttttcag tttttcggca ccgatatcac ccatatcaac ttccagtttg
    66601 tgatcgtctg gaacgataga acacactgag tcttcaatgg tgccgtaaaa acgtgcagta
    66661 ccgttgttac ccgcagcgat tgttgccgta gaggtgaaca cgcccatcac cattgccgca
    66721 gcaatagctg ttttaacaaa cttactcatt atttgtcctt aaactataaa taattaaaaa
    66781 tataaacatt gcaatacatt gattcagtca atagccaatg tttataccca gccattgaat
    66841 acggcatgcg acccaaaaag aacaggtcgt agaaaattgt attcattggt gtggggccgc
    66901 atagtgatgt ttccacaccc ctgatccaat ttaattttac taaagagtaa atgtagattt
    66961 aattaatata ttgacagaag aaaggttatc ttaaggacat ccttaaaaaa tacggaataa
    67021 gtatttagaa aaaatagaaa gtatatcaat aatgaatatc aacagcttaa aaaacaaaca
    67081 atgaacatat aatgcgattg gcatttatcc acttttgaca atataatata taaacactat
    67141 agccagaatt taatcaagtt tcaattacat cctgcctgat tattcaactc tattttctct
    67201 attatctgta ttaataaatg acaatgagtg tcaaacagat attattcatt tcaacatgta
    67261 actttgggaa aaatcaagat acgcgcagaa gaataatttc cgaatgaatg tagaaagtgt
    67321 tttatttata gataaggaga gtcttcgata gtagtgaggt tgacagatat ccgctgccta
    67381 atgagcaatt atgcccaatg aatctacctc attgggcata atttggaaca catcagatag
    67441 cttttgccat cttcagatta cacgggctca tctgccagtt gaatgtaccg tttccgcttt
    67501 catcaagcgt tacgctggca atggccgagg tggtaaacat aggcggcgtt tcgcccggac
    67561 ataactcggc aaccagatac ccgactaacg gtaagtgaga gatcaccagt accgaagcaa
    67621 caccttcatt ggtcaacgcc tgcaaatagg cactgaccag accaacatcg ccgcagggcg
    67681 ttaactccgg cagaacttct gcactggaag gcaggttcag acaatcccct acttcttcca
    67741 gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc actttttgac
    67801 ctttcagcca gttcgccatc aggcgagatt cgtcacaacc attagtggtc agaggacgaa
    67861 ccgaatcact ggcggaatcg agggctgcgt cgccgtgacg catgataaaa acttgcatat
    67921 tgcaccgctt ttgttaacca gattcgcccg ttttctttca tctgaaacca gagaaagaaa
    67981 acggtggccg gcattgtgcc ttatccattc accgaatgaa acgctgtttt ttacctcaat
    68041 ggcgtaagta tagtcaatat gcgtttacat tttgaccaac actccgccat tcagcgcgga
    68101 ttcatatagc tttgaccttc ttattgcagg tcagttgcag ttgttttcca aaaactttcc
    68161 ccacgcgcgc ccatctcgac caaacgctcg caaggggtaa aacgggaacc atactgcgtg
    68221 gccagtcgtt gcattattgc aaccacttcg cccgcgccga gagaatcgat atagcggaac
    68281 ggtccaccga gaaatggcgg aaaaccaatg ccaaataccg caccaatatc cccgtcacgc
    68341 acgctacgga taacctgctc atcaacacaa cgtactgctt cattcagcat caacatcaca
    68401 caccgttcag caacctgcgg tgcggagagt cacccctgcc cttgtgcgcc aatgagcggg
    68461 taaatggcag gatcgacctg ttttttgctt ttacgccctt tctgaccata aagatagaaa
    68521 ccccgaccat tttttctgcc tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca
    68581 ggcgcgctaa aacgttctcc ataagcggct tccagtacag gaataatttt agtcccggtg
    68641 tcgattccta cctcatccaa aagttggatt gggcctaccg gaaaaccaaa tttcactagc
    68701 gcggcatcaa tgtgctctac ccgttcacct tcggtcagca tgcggatagc ttcattaatg
    68761 taaggcgcta agatgcgatt gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt
    68821 ttgccctgtt ttttcgccag ttttaccgta gtggcgatgg tttgcgccga tgtacccgca
    68881 tgaggaataa tctctaccag cggcattttt tccaccggac tgaagaaatg caggccaata
    68941 acttgctcag gtcgcgcggc gtgagcggcg atatcaccaa tcggtaaaga tgacgtgttc
    69001 gaagcaaaaa tggtatgagc ggcgcaattt tgctccactt ccgccaccat ctgttgtttt
    69061 aattcgagat tttcaaacac cgcttcaata atcagatcgc gatgggcaaa gccgcgatag
    69121 tccgtcgttc cggagattaa tgccagctgt ttgtcacgtt cgctggcttt gagatgacga
    69181 cggcgaactt tgccctccag ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc
    69241 gggttgatat ctttaattct gaccggaagc cccgctttac aagcagtgac ataagcaata
    69301 ccgccgccca tcaagccacc acctaaaatt cccacgctgt ttaatgccgc aggcggtgca
    69361 tcactaccgg gatctttctt cacgtccgta ctggcaaaaa agatactacg caaagcttgc
    69421 gattgcggcg tcatcgccag ttcgccaaag gcgcgagctt cagcgtcata gccgctgctg
    69481 gtgccctgcg ctaatcccgt ttcaacaacc tccaggatgc gttctgtcgc cggataattg
    69541 ccttgagttt tgtgttctgt tttcttgccg accattttga acagcagcgc acgacctaac
    69601 ggccccgcca gaatacgctc gcgtacaggt agagggcggg aagatgggcg atccttcttc
    69661 gccagctcaa cagcggcttc cagcaaaatg gagtgcggaa caacgtcatc caccagcccc
    69721 agctttaatg cctgtttcgc ccgaagttgt tttccggtga gaatcatctc taatgctgta
    69781 ctgacgccta tcagacgcgg taaacgctgg gtgccgcctg aaccgggtaa caatccaagt
    69841 tgtacttcag acaaaccgag caccgtttta ggatcgtcag tacaaacgcg accgtggcac
    69901 gccagcgcca gctccagccc accgcccagg caagcgccat gaatagccgc gataaccgga
    69961 atgggcaaag catgaatctc cgccatcaac tgttggccct gccgcgccag tgcttccgct
    70021 tcttgcgccg ttttgcagtt gccaatcatg ttgatgtctg ctccggcgat aaaattgtcc
    70081 ggtttcgcgg agataaacac cacgccgcgc aactctttgt tttcacggag ttgcttaata
    70141 atggcgcgca cctgcgaggc aaattccgcc ttcagggtat tcattttctc atccggtacg
    70201 tcgatggtga tgatagcaat gttgtccaga cgaacattaa gagtaaacgc tgatgccatt
    70261 tccattattc cgcctccaga accattgccg cgccaagccc accggcagca caggcggtaa
    70321 ctaaaccaaa tccaccgccg cgacggcgaa gttcatgcaa tgtctgggta atcatccgcg
    70381 cgccggtcgc cgcgaaggga tgtccgtaag caatcgaacc gccaagcacg ttaaatttgc
    70441 tatcgtccac ttcgccagtg gcatgtgcac gccccagtac ttcacgagca aaacgttcac
    70501 tacccagcaa ctgaatattc gccagtgtct gagctgcaaa ggcttcgtgc atatcgatca
    70561 atgtcagttc gctcatcgtc aaaccggcac gttccagcgc cagcggtgtt gaccaggctg
    70621 gaccgagcaa catgtcctgc cagacatcaa tcgcagtaaa tgcgtagctg cgcagatacc
    70681 ccagcggcac cagccctaat tctttcgccc gggattcagt catcaggatc accgctgccg
    70741 cgccatcggt cagcggcgta ctgtttgccg ccgttaccgt tccgtgtttg cgatcaaacg
    70801 ccgggcgcag ctttgcgtaa tcggcaagcg aggaattacc gcgaatattg ttgtcttcga
    70861 caagcggttg tttataagga gggataaagg cagtcatcac ctcttctttg agttttccgt
    70921 ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc taatgcatct tgctgttctc
    70981 gggtgatgcc gtaggttttc gccatttgtt cggcggtgtc gcccatccgc aagccagtag
    71041 aatattctgc taccgcaggc ggtacgggca ttaagtcgcg caaacgcagg cgagagaaga
    71101 gtttcaggcg ctggctcatg gttcgagctt tgttgacatc aaccagcacg tgcgccagtt
    71161 ttttactgac gccaattggc aatacggaag aggaatctgc cccaccggca atccccgctc
    71221 ggatagttcc cgccatcagg ctttctgcga cgtttgcaac cgcctggaaa ctggtagcgc
    71281 aagcgcggct gacgctgtaa gcatcggtat gcacattcat tccggtaccg agaacaattt
    71341 cacgcgcaat gttgggggct tcaggcattt gtacgacctg accaaagacc agctgttcaa
    71401 tcacttcagc ggggatctcg ctgcgtgcca gcagttcgcc taccaccatc ttccctaaat
    71461 caaccgtggg aatgccatga aaagccgtcg cctggcgggc aaaaggcgta cgtaaaccgc
    71521 taacaatggc gatacgatcg ccctggcggg taaccagtgg taaaacctga cccataacac
    71581 tcccctgtaa aaaaataaat aaagtggtct gacctgatca tagtcttaac cattttttta
    71641 catttagcca agcggagaaa aaggaaagtg ggagctatga cacagtgaag acagaagaaa
    71701 gaaaagaaaa cgcccctgcc agcaactgac aggggcgtac gtacccgaga ggaattaacg
    71761 cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat cgatatccag
    71821 gcgcacaccg tcagactctt cagtgaaagt cggggagatt ttgcacggtt cggattccac
    71881 gctacgggat ttttcagtca gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa
    71941 cacgcggctg taagacgcgg tgcagtcgga gttgtccata atggtgccaa catccataca
    72001 gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc tctgtatgtg
    72061 cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt gctgctccag
    72121 ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg atctaaaaca
    72181 aaattcaccc gaatccgcga gtgcgcaacc tccaaatttt gccagctgga tcgcgtttct
    72241 tagatcatat ttgaaaaaag atggaaacat acttgcaaca ttctagctgg tccgacctat
    72301 actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc gcgctcctgt
    72361 tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc atgagccaga
    72421 aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc acccaggcct
    72481 ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg gcttattcag
    72541 gggaaggcgc aattgccgat gatgcaggta acgttagccg taaccccgca ttgattacca
    72601 tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgacccggat gtgaatatca
    72661 gcggaacgtc tccgtctggt cgtagcctga aagccgataa catcgcgcct acggcatggg
    72721 ttccgaacat gcactttgtt gcaccgatta acgaccaatt tggttggggc gcttctatta
    72781 cctctaacta tggtctggca acagagttta atgatactta tgcaggcggc tctgtcgggg
    72841 gtacaactga ccttgaaacc atgaacctga acttaagcgg tgcgtatcgc ttaaataatg
    72901 catggagctt tggtcttggt ttcaacgcca tctacgctcg cgcgaaaatt gaacgtttcg
    72961 caggcgatct ggggcagttg gttgctggtc agattatgca atctcctgcc gggcaaactc
    73021 ctcaagggca agcattggca gctaccgcca acggtatcga cagtaatacc aaaatcgctc
    73081 atctgaacgg taaccagtgg ggctttggct ggaacgccgg aatcctgtat gaactggata
    73141 aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa aattgacttc aaaggtaact
    73201 acagcagcga tcttaatcgt gcgtttaata actacggttt gccaattcct accgcgacag
    73261 gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc tgaaatgtgg gaagtgtcag
    73321 gttataaccg tgttgatcca cagtgggcga ttcactatag cctggcttac accagctgga
    73381 gtcagttcca gcagctgaaa gcgacctcaa ccagtggcga cacgctgttc cagaaacatg
    73441 aaggctttaa agatgcttac cgcatcgcgt tgggtaccac ttattactac gatgataact
    73501 ggaccttccg taccggtatc gcctttgatg acagcccagt tccggctcag aatcgttcta
    73561 tctccattcc ggaccaggac cgtttctggc tgagtgcagg tacgacttac gcatttaata
    73621 aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg tcagagcgtg aaaattaacg
    73681 aaggcccata ccagttcgag tctgaaggta aagcctggct gttcggtact aactttaact
    73741 acgcgttctg ataacgcgtt cgcctagata aagtcacctg ctatgcaggt gactttaact
    73801 cccccacttc accgaaagta gtttcctcat tgcttgcctc ctgagttttg caaacagcct
    73861 gatggcagct tgccctttca aatcagtaag tagtatccac agacaaacct gaagggaaat
    73921 gcatattttc aagcgttctg ctcgtccttc tcaaagagtt tacttttctg catttccagg
    73981 atactcaacc cccctggcta ttgtgcgctt atacactcaa attaaagata ggttctaaat
    74041 aaatgagcgt tttttgatag tctatttcat taggtaatat atatttgtaa caaatcaatc
    74101 aacatggaat aaaatcatgc taccatctat ttcaatcaac aataccagcg cagcttaccc
    74161 agaatccatc aatgaaaata acaatgatga aattaatgga ttagtacaag agttcaaaaa
    74221 ccatttttaa tggtaaggaa ggaataagca cctgtattaa acatctactt gagcttataa
    74281 aaaacgccat acgagtaaac gacgatcctt atagatctaa tattaataat tcctcagtta
    74341 cttatattga tattggctcc aatgatacag accatattac tattggtatc gacaaccaag
    74401 aaccaataga attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg
    74461 acaacatagt tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa
    74521 aagaaatata tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac
    74581 atacggtaac ggaatttgat gagagcggga aaagcgaacc aacggactta tttacctggt
    74641 acggtaaaga taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg
    74701 attacttatt tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta
    74761 ttaatggtga tagtctcacc caatattgta gtgaaaacgc caggagtgct tcagcgtggt
    74821 ttgaaagcag taaagctatc atggcagaat catttgcaac tggttccgat catcaggttg
    74881 taaacgagct caacggggaa agactgagag aacctaacga agtttttaaa cgtttaggtc
    74941 gagcaataag atataatttt caagtggacg atgcaaaatt tagacgcgat aatgtaaaag
    75001 aaataatttc tactttattc gctaacaaag ttgacgttga ccatcctgaa aataaataca
    75061 aagactttaa gaatctcgaa gataaagttg aaaaaagact tcaaaatcgc cagacgaaat
    75121 atcaaaatga aattaatcaa ctatctgcac taggtgttaa ttttgatgat atttaaaatt
    75181 atttttccag ttttttgatt tttcaacgcc tgaatatcgg aaatagtcag tgacaaacat
    75241 cagtattttt acatcattac gtccgggtca ttgtcggttt atctcctttt acttgtggta
    75301 attgagggat aacaacaaaa aaggtgagtt ttgcgactca ccttttttat ttgtttctta
    75361 ttcagaatcg atatctttta aatcatcctg aatcgcttgc gcgttcggat tttcctgcgg
    75421 tttgagttcg ccgccattag cgatgaaatc atgacgctgg aagtacgctt cgcgcaccat
    75481 aatataagga tcggacgact gacgcagcag accatcggaa tccagcagct gagcgcgggt
    75541 ttcgatccct tcaagcgtcc atttacccac agacatcggc caggtcagcc aggaaagaac
    75601 cgggtaaaga gcatccgcca tatcaccacc gtcatcacgc agcgtgaagc taccgtagaa
    75661 cggtaactga acgtaaggcc cataacccac gccataatga ccaagcgtac taccgaagcg
    75721 gtgaggttca gtccgttgca gtttcgggtt cgccatccct gcaacatcaa taaagccgcc
    75781 catccccaaa atggtgttca ggaaaaagcg ggtaaagtgg accatcccct gataagggtc
    75841 gccctgcaag aagtagttaa ccatcaccgc aggttcttca aggttgccag taaagttgct
    75901 caaaccatta cgcgccggtt gcggaacata atcacgccag gcgacagcga ccggtcgaac
    75961 aatatacggg tctaatacgt tgaagttgaa gttgtacatg gtgcggttga acccttctaa
    76021 cgggtcagaa cgcccttgct gatctgtacc ggaactcgca caccccacca gaagcgtagt
    76081 tcccagagca agcgccgaca ggcgaagctt cataaatgtc tccctgtttt tttatggctt
    76141 atgcagtttg ccatccatga cggaacgata ccgtatccgc ctgtttaggt gtgggcgatt
    76201 gtaacagcac gtcaactgat gtccagacgc cctgatttgc tgatttgatc atagcctggt
    76261 aatcgccgcc ctgtaggcta cttgattcta tagaaacaga aaaaggcaaa cgcagccttt
    76321 tctacatttt cagagtaact cccgccagtt gcgagcaaaa aagccgctac gctttagcta
    76381 tacgtgctaa ttcaagagaa gagaccatgg acaacgacaa aattgatcaa cacagcgacg
    76441 aaattgaagt tgagagcgaa gaaaaagagc gcggcaaaaa aatagaaata gatgaagacc
    76501 gactcccctc ccgagcgatg gcaattcatg agcatatccg ccaggatggt gaaaaagagc
    76561 tagaacgcga cgcaatggcg ctactgtggt cagccattgc ggcgggtctg tcgatgggcg
    76621 cttcgttact ggcaaaaggg atatttcatg ccgaactgga aggtgtgccg ggcagcttct
    76681 tgctggagaa tctcggttat acctttggtt ttattatcgt cataatggcc cgccagcaat
    76741 tatttaccga aaacaccgtg actgcggtac tacccgtcat gcaaaaaccg acaatgagca
    76801 acgttggctt acttatgcgg ttatggggag tcgtgctgct gggtaatatt ctcgggactg
    76861 gtattgctgc gtgggcattt gaatatatgc ctatcttcaa tgaagaaact cgcgatgcat
    76921 ttgtcaaaat cggcatggat gtgatgaaga acacccccag cgagatgttt gccaacgcga
    76981 tcatttccgg ctggctgatc gccactatgg tatggatgtt tcctgcagca ggtgcggcaa
    77041 agattgtggt gattatattg atgacctggc ttattgccct gggtgacacc acccatatcg
    77101 tggtcggttc tgttgaaatc ctctatctgg tgtttaacgg tacgctgcac tggagcgatt
    77161 tcatctggcc cttcgcacta cctactttag cggggaacat ctgcggcggc acctttatct
    77221 tcgcgttaat gagtcatgca cagattcgta acgacatgag caacaagcgt aaagcagaag
    77281 cacgccaaaa agcagaacgt gcggaaaaca ttaagaaaaa tgataaaaac ccagcataaa
    77341 tggcgagggt ttaagcaatc gagcggcagc gtacttaccc cgcagtccat tagcgggtat
    77401 actcgccgca ttgtcctctt agttaaatgg atataacgag cccctcctaa gggctaattg
    77461 caggttcgat tcctgcaggg gacaccattt atcagttcgc tcccatccgt accagtccgc
    77521 aaaatcccct gaatatcaag ccttccgtat attcacagtt cgtcatggtt cgcgtcagat
    77581 cgttgacagc cgcactccat gacgggtaaa aagtggataa aataatttta cccaccggat
    77641 ttttacccat gctcaccgtt aagcagattg aagcagcaaa gccgaaagaa aaaccatacc
    77701 gccttctcga tggtaatggc ctgtaccttt atgtccctgt atccgggaaa aaggtatggc
    77761 agcttcgcta caagattgac ggtaaggaga aaatcctgac cgtcggaaaa tatccgctta
    77821 tgactttgca ggaggcaagg gataaagcat ggactgcgag gaaagacatc tcggctggca
    77881 tcgatccggt aaaggcgaaa aaggcttcgt ctaacaacaa ttcatttagt gcgatttaca
    77941 aggaatggta cgaacacaag aagcaagtct ggtcagtagg gtatgcgact gaacttgcaa
    78001 aaatgtttga tgacgacatt ttacccatca tcggcggcct tgagattcag gatattgagc
    78061 cgatgcaact gctggaagta atccgcagat ttgaagatcg cggtgc
//
LOCUS       DAEBXD010000003        77882 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEBXD010000003 DAEBXD010000000
VERSION     DAEBXD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77882
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.003"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="KJL94_000182"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="KJL94_000182"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBD4408700.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="KJL94_000183"
     CDS             complement(1672..2730)
                     /locus_tag="KJL94_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4408701.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="KJL94_000184"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="KJL94_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="KJL94_000185"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="KJL94_000185"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBD4408702.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KJL94_000186"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KJL94_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBD4408703.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KJL94_000187"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KJL94_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBD4408704.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="KJL94_000188"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="KJL94_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBD4408705.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="KJL94_000189"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="KJL94_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBD4408706.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KJL94_000190"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KJL94_000190"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBD4408707.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KJL94_000191"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KJL94_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBD4408708.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KJL94_000192"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KJL94_000192"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBD4408709.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KJL94_000193"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KJL94_000193"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBD4408710.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="KJL94_000194"
     CDS             complement(17699..18322)
                     /locus_tag="KJL94_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBD4408711.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="KJL94_000195"
     CDS             18619..18894
                     /locus_tag="KJL94_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408712.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="KJL94_000196"
     CDS             complement(18971..19213)
                     /locus_tag="KJL94_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBD4408713.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="KJL94_000197"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="KJL94_000197"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBD4408714.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="KJL94_000198"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="KJL94_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBD4408715.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="KJL94_000199"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="KJL94_000199"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBD4408716.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="KJL94_000200"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="KJL94_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="KJL94_000201"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="KJL94_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBD4408717.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="KJL94_000202"
     CDS             24840..25697
                     /locus_tag="KJL94_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4408718.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25700..27931)
                     /gene="ptsP"
                     /locus_tag="KJL94_000203"
     CDS             complement(25700..27931)
                     /gene="ptsP"
                     /locus_tag="KJL94_000203"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD4408719.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETE
                     LTF"
     gene            complement(27956..28993)
                     /gene="ypdE"
                     /locus_tag="KJL94_000204"
     CDS             complement(27956..28993)
                     /gene="ypdE"
                     /locus_tag="KJL94_000204"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD4408720.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28993..30076)
                     /gene="ypdF"
                     /locus_tag="KJL94_000205"
                     /pseudo
     CDS             complement(28993..30076)
                     /gene="ypdF"
                     /locus_tag="KJL94_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30091..31338)
                     /locus_tag="KJL94_000206"
     CDS             complement(30091..31338)
                     /locus_tag="KJL94_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBD4408721.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31360..31686)
                     /locus_tag="KJL94_000207"
     CDS             complement(31360..31686)
                     /locus_tag="KJL94_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBD4408722.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31905..32870)
                     /gene="glk"
                     /locus_tag="KJL94_000208"
     CDS             complement(31905..32870)
                     /gene="glk"
                     /locus_tag="KJL94_000208"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBD4408723.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33074..34330
                     /locus_tag="KJL94_000209"
     CDS             33074..34330
                     /locus_tag="KJL94_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBD4408724.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34445..34771
                     /locus_tag="KJL94_000210"
     CDS             34445..34771
                     /locus_tag="KJL94_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD4408725.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34912..36150)
                     /locus_tag="KJL94_000211"
     CDS             complement(34912..36150)
                     /locus_tag="KJL94_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBD4408726.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36486..37688
                     /gene="nupC"
                     /locus_tag="KJL94_000212"
     CDS             36486..37688
                     /gene="nupC"
                     /locus_tag="KJL94_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBD4408727.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37738..39850)
                     /locus_tag="KJL94_000213"
                     /pseudo
     CDS             complement(37738..39850)
                     /locus_tag="KJL94_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40058..40133)
                     /locus_tag="KJL94_000214"
     tRNA            complement(40058..40133)
                     /locus_tag="KJL94_000214"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40098..40100),aa:Ala,seq:ggc)
     gene            complement(40173..40248)
                     /locus_tag="KJL94_000215"
     tRNA            complement(40173..40248)
                     /locus_tag="KJL94_000215"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40213..40215),aa:Ala,seq:ggc)
     gene            40469..40828
                     /locus_tag="KJL94_000216"
     CDS             40469..40828
                     /locus_tag="KJL94_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD4408728.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40830..41222
                     /locus_tag="KJL94_000217"
     CDS             40830..41222
                     /locus_tag="KJL94_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD4408729.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41274..42689)
                     /gene="gltX"
                     /locus_tag="KJL94_000218"
     CDS             complement(41274..42689)
                     /gene="gltX"
                     /locus_tag="KJL94_000218"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBD4408730.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            42948..43023
                     /locus_tag="KJL94_000219"
     tRNA            42948..43023
                     /locus_tag="KJL94_000219"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42981..42983,aa:Val,seq:tac)
     gene            43068..43143
                     /locus_tag="KJL94_000220"
     tRNA            43068..43143
                     /locus_tag="KJL94_000220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43101..43103,aa:Val,seq:tac)
     gene            43148..43223
                     /locus_tag="KJL94_000221"
     tRNA            43148..43223
                     /locus_tag="KJL94_000221"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43181..43183,aa:Lys,seq:ttt)
     gene            43254..43379
                     /locus_tag="KJL94_000222"
     ncRNA           43254..43379
                     /ncRNA_class="other"
                     /locus_tag="KJL94_000222"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43655..43996
                     /locus_tag="KJL94_000223"
     CDS             43655..43996
                     /locus_tag="KJL94_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBD4408731.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(43987..44913)
                     /locus_tag="KJL94_000224"
     CDS             complement(43987..44913)
                     /locus_tag="KJL94_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4408732.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45003..46001
                     /locus_tag="KJL94_000225"
     CDS             45003..46001
                     /locus_tag="KJL94_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBD4408733.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(45998..46216)
                     /locus_tag="KJL94_000226"
     CDS             complement(45998..46216)
                     /locus_tag="KJL94_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBD4408734.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46218..48233)
                     /gene="ligA"
                     /locus_tag="KJL94_000227"
     CDS             complement(46218..48233)
                     /gene="ligA"
                     /locus_tag="KJL94_000227"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBD4408735.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48304..49290)
                     /gene="zipA"
                     /locus_tag="KJL94_000228"
     CDS             complement(48304..49290)
                     /gene="zipA"
                     /locus_tag="KJL94_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBD4408736.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49520..50281
                     /gene="cysZ"
                     /locus_tag="KJL94_000229"
     CDS             49520..50281
                     /gene="cysZ"
                     /locus_tag="KJL94_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBD4408737.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50466..51437
                     /gene="cysK"
                     /locus_tag="KJL94_000230"
     CDS             50466..51437
                     /gene="cysK"
                     /locus_tag="KJL94_000230"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBD4408738.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51821..52078
                     /gene="ptsH"
                     /locus_tag="KJL94_000231"
     CDS             51821..52078
                     /gene="ptsH"
                     /locus_tag="KJL94_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBD4408739.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52123..53850
                     /gene="ptsI"
                     /locus_tag="KJL94_000232"
     CDS             52123..53850
                     /gene="ptsI"
                     /locus_tag="KJL94_000232"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBD4408740.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53891..54400
                     /gene="crr"
                     /locus_tag="KJL94_000233"
     CDS             53891..54400
                     /gene="crr"
                     /locus_tag="KJL94_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBD4408741.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54442..55293)
                     /gene="pdxK"
                     /locus_tag="KJL94_000234"
                     /gene_synonym="thiJ"
     CDS             complement(54442..55293)
                     /gene="pdxK"
                     /locus_tag="KJL94_000234"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBD4408742.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55398..55772
                     /locus_tag="KJL94_000235"
     CDS             55398..55772
                     /locus_tag="KJL94_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBD4408743.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55805..56528
                     /locus_tag="KJL94_000236"
                     /pseudo
     CDS             55805..56528
                     /locus_tag="KJL94_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56732..57643)
                     /gene="cysM"
                     /locus_tag="KJL94_000237"
     CDS             complement(56732..57643)
                     /gene="cysM"
                     /locus_tag="KJL94_000237"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBD4408744.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57777..58874)
                     /gene="cysA"
                     /locus_tag="KJL94_000238"
     CDS             complement(57777..58874)
                     /gene="cysA"
                     /locus_tag="KJL94_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBD4408745.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58864..59739)
                     /gene="cysW"
                     /locus_tag="KJL94_000239"
     CDS             complement(58864..59739)
                     /gene="cysW"
                     /locus_tag="KJL94_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBD4408746.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59739..60572)
                     /gene="cysT"
                     /locus_tag="KJL94_000240"
     CDS             complement(59739..60572)
                     /gene="cysT"
                     /locus_tag="KJL94_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBD4408747.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDS
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60572..61588)
                     /gene="cysP"
                     /locus_tag="KJL94_000241"
     CDS             complement(60572..61588)
                     /gene="cysP"
                     /locus_tag="KJL94_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBD4408748.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61892..62683)
                     /gene="ucpA"
                     /locus_tag="KJL94_000242"
     CDS             complement(61892..62683)
                     /gene="ucpA"
                     /locus_tag="KJL94_000242"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBD4408749.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62870..63670)
                     /gene="murR"
                     /locus_tag="KJL94_000243"
     CDS             complement(62870..63670)
                     /gene="murR"
                     /locus_tag="KJL94_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBD4408750.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63834..64730
                     /gene="murQ"
                     /locus_tag="KJL94_000244"
     CDS             63834..64730
                     /gene="murQ"
                     /locus_tag="KJL94_000244"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBD4408751.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64734..66158
                     /gene="murP"
                     /locus_tag="KJL94_000245"
     CDS             64734..66158
                     /gene="murP"
                     /locus_tag="KJL94_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBD4408752.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66338..67237)
                     /gene="yfeX"
                     /locus_tag="KJL94_000246"
     CDS             complement(66338..67237)
                     /gene="yfeX"
                     /locus_tag="KJL94_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBD4408753.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67333..67908)
                     /locus_tag="KJL94_000247"
     CDS             complement(67333..67908)
                     /locus_tag="KJL94_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBD4408754.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67969..68418)
                     /locus_tag="KJL94_000248"
     CDS             complement(67969..68418)
                     /locus_tag="KJL94_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBD4408755.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68405..68830)
                     /locus_tag="KJL94_000249"
     CDS             complement(68405..68830)
                     /locus_tag="KJL94_000249"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD4408756.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69044..69913
                     /gene="amiA"
                     /locus_tag="KJL94_000250"
     CDS             69044..69913
                     /gene="amiA"
                     /locus_tag="KJL94_000250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBD4408757.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69917..70816
                     /gene="hemF"
                     /locus_tag="KJL94_000251"
     CDS             69917..70816
                     /gene="hemF"
                     /locus_tag="KJL94_000251"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBD4408758.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70822..71874)
                     /gene="eutR"
                     /locus_tag="KJL94_000252"
                     /pseudo
     CDS             complement(70822..71874)
                     /gene="eutR"
                     /locus_tag="KJL94_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71920..72420)
                     /gene="eutK"
                     /locus_tag="KJL94_000253"
     CDS             complement(71920..72420)
                     /gene="eutK"
                     /locus_tag="KJL94_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBD4408759.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72433..73091)
                     /gene="eutL"
                     /locus_tag="KJL94_000254"
                     /pseudo
     CDS             complement(72433..73091)
                     /gene="eutL"
                     /locus_tag="KJL94_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
     gene            74273..75064
                     /locus_tag="KJL94_000255"
     CDS             74273..75064
                     /locus_tag="KJL94_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408760.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75784..76179
                     /locus_tag="KJL94_000256"
     CDS             75784..76179
                     /locus_tag="KJL94_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD4408761.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76197..76460
                     /locus_tag="KJL94_000257"
     CDS             76197..76460
                     /locus_tag="KJL94_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408762.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76483..77025
                     /locus_tag="KJL94_000258"
     CDS             76483..77025
                     /locus_tag="KJL94_000258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408763.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77164..77451
                     /locus_tag="KJL94_000259"
     CDS             77164..77451
                     /locus_tag="KJL94_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408764.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77490..77696
                     /locus_tag="KJL94_000260"
     CDS             77490..77696
                     /locus_tag="KJL94_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408765.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        20194 a        19916 c        18590 g        19182 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatat
    25861 tctccagcgc cagcagtggg cgaacgtctt cttccggcgt aaaggcggtg agtaacgctt
    25921 caatttcctg cgcactgcgg cattcacatg cctgacgcgc cagttcccga cacgcctcgc
    25981 tatccagttg acgaagctgg cttttcaccg ccggaatacg cgggctactc atactcagct
    26041 catccaggcc cagcccaagc agtagcggca gataacggct ttcaccgccc agttcaccgc
    26101 aaatgcctac ccatttgccc cgctgatgcg cagtggtaac tatttgctgc aacatgcgca
    26161 ggaacgatgg cgtaatcggg ttatatagcg gcgatacgcg cgggttatta cgatcgaccg
    26221 catacagata ctgggtcata tcgttggagc cgatactgaa gaaatcgacc tcatcgcaga
    26281 agtggtcgat gatgtagcac accgacggca cttccaccat gatcccaagc gtaatcgtct
    26341 ctgcatgacg caggccatcg cgcttaagct caacgatcgc tttttggatc tcgcctttca
    26401 cccataagat ctgatcgagg ctgtgaacca tcgggatcat caactgggcg ttgccgaaac
    26461 tggcggcgcg caaaatggcc cgcagttgag tgcggaacag gccagcaaat tccgggtaaa
    26521 tacgtaccgc gcgatagccg aggaacgggt tctcttcctg gggaatattc agataaggaa
    26581 tgcttttatc gccgccgatg tccatcgtgc ggaagataat cggcttgtcg cccgccgcca
    26641 gtagcacctg ctggtaggct tcaaattgct cctgctcgtc cggcgcgctg tcgcgatcca
    26701 tatagagcat ttcggtacgg aacagcccga caccttccgc gccgttggca aacgcgcctg
    26761 gcgcttccag agcggtgccg atattcgccg caatatcaat acgcttgtta tcacgggaat
    26821 aggccagctg cgcggcagcc tgcgcctgtt gtttttggcg tttatccgcc agcgtctgcg
    26881 cgacctgata ataaccgctc accgcgtcat tcgggttaat cgccagcacg ccgcactggg
    26941 cgtcaagcac tgcaggttgc ccggcataac gggcaatcgc atccagcggc aagccactca
    27001 gtaccgggat cgccgaggca cgggccagaa tcagtgtatg cgaggtgcgc ccggttttct
    27061 ccagaatcat acccgcaaga tttttcaaat caaggctcaa aaactggctt ggggttaaat
    27121 cttcagccac cagaatggtc ggtttttcca gcaccagatt gttgcgcggc ttcagttccg
    27181 gccaggtgat atgcagcaac tgttcgctga tatcgcgaat gtcgctgacc cgctcgcgca
    27241 gataatcgct ggcagaagca gatagtttgg cgcaaacctg ctccatattg ctgatgatcg
    27301 ccgcccccag tccctgatgc tgttctgtca tcaggcgacg gatattgcct gcaaattcat
    27361 catcctgaat cagcgacaaa tgagcgctga ggatagtttt gctttcgccg tcacgctcac
    27421 gcaattgctg attcagttgc tcggcaagcg ttgccaggct gtgctccagc cgggtggaat
    27481 cttgcgcact ggcggggatt gcccgataac tgtcgaggct gtcgctttgt aacagggtca
    27541 gcgtacccac gccgacgccg cttgccagca cattgccgta cagtaaatcc gggttaaggc
    27601 ggcttaatga acgcggcagc ggatgcgccg tcagttctgc ctgcgtcggc tgaacgctgt
    27661 cgctgtcgat aaagcgcacc aggatgtact cttccagcac gcgccgcgcc tgctcttcat
    27721 cgctgccgct aatgttcagg ctgcaactgt cattaaataa ggtgccagtt ccaatcaacg
    27781 ccagcgagct tttagcatca gcctttgcgt tctggcgatg gttgataaag gtgatttcgc
    27841 tttgccactg gctgcactgt tctttaagtt cccacgccgg acgagcatgt agaccgttag
    27901 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    27961 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28021 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28081 gggtggcagg ccctattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28141 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28201 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28261 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28321 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28381 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28441 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaactgagg cgatcatcaa
    28501 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    28561 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    28621 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct ggaattttgc
    28681 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    28741 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    28801 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    28861 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    28921 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    28981 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29041 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29101 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29161 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29221 gaaatagtcg ccaaatccag cctcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29281 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29341 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29401 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29461 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    29521 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    29581 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    29641 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    29701 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    29761 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    29821 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    29881 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    29941 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30001 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30061 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30121 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30181 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30241 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30301 catatatgac ggaatagcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30361 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30421 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30481 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    30541 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    30601 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cagtacggat
    30661 accgccgttg atccagccac caaagggagt gatgacgtag tacatcgcca gcatgacaaa
    30721 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    30781 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    30841 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    30901 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    30961 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31021 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31081 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31141 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31201 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31261 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31321 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31381 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31441 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31501 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgga tgccgtccgc
    31561 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcacctg
    31621 agcggccata aaagtgtgag ctaggcccat tgggcaggca cataaggcaa tcagtttctt
    31681 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    31741 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    31801 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    31861 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    31921 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    31981 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32041 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32101 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32161 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32221 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32281 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32341 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32401 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32461 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    32521 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    32581 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    32641 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    32701 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    32761 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    32821 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    32881 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    32941 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33001 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33061 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33121 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33181 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcacc
    33241 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33301 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33361 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33421 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33481 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    33541 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    33601 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    33661 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    33721 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    33781 agggatggtc gccgtatggt gcttaccacg gttgcacgcg atgatgaatc aaatgaaaaa
    33841 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    33901 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    33961 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34021 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34081 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34141 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34201 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34261 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34321 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34381 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34441 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34501 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    34561 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    34621 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    34681 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    34741 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    34801 atgcgacgct agcgcgtctt atcaggccta caaaccggct ctttaacgcg tagggcggat
    34861 aaggcgttta cgccgcatcc gccaatagtg ccagatgcga cgctcattca actacaatcc
    34921 cagcgccgtc cccaccaaca accagatatt cagcgccacg accagcacta caatcaccca
    34981 gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc atcaacttgc tgtcactggt
    35041 gaaaatcagc agtggaacca gcgccagggc gataccaaaa cttaacagca cctgactcat
    35101 aaccagaatc cgtgtcggat ctaatcccat cagaatgaca ataaatgacg gcaacatggt
    35161 gactgtacga cgcacccaca gcgggatatg gaagcgaatg aatccctgca tcaccacctg
    35221 ccccgccagt gtccccacca ccgttgagga cagtccggca gcaaccagac ttaacccaaa
    35281 gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc agataagcct catcaagatc
    35341 ggcaacacca gtatgaccag aaaagtggaa cgccgccgca gctgtagcca tcatcaccag
    35401 attgacaaaa ccggcaatcg tcatggcgat agccacatcc catttggtgg cggaataacg
    35461 ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg
    35521 cggcataatc gtcgccccta acacgcctgc tgccaggaag accgcctccg aagtaggtaa
    35581 actcgggatc accattcctt tacccagctg cgccaggtta ggctgggaga aaatcaactc
    35641 gacaatgtaa gccgcggcaa caaacaacag taacccgcca atcactttct ccagcggttt
    35701 ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc
    35761 ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc gccgcaccaa taaattccgc
    35821 caggtcggtt gccatcgcaa taatttctgc ctgaacccaa tagaaccaca ctacgggacg
    35881 cggatagtga tcacgaatct gctccgccag atttttaccg gtggcaatcc ctagtttggc
    35941 agagaggatc tgaatcagca tcgccatcag gttggcccaa acgacaaccc acagtagctg
    36001 atagccgaag ctagcacccg cctgaatatt ggtcgcaaag ttaccgggat cgatataacc
    36061 aatcgccgca atgaacgcag gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc
    36121 gctgctactc tcaacgcgat agttcgtcat cttgtgcctc taaaacatag cctttgctat
    36181 gtttcatgct atgctaaacg agaatgatta tcaaattcat ttaaatggat tgtggtgatt
    36241 tctctgatag accaagatta tgattacgaa aagattgatg acttgaatgt tatggttatg
    36301 tttaatgtta gcacatttac ataactttca gcttccatac acaacatagc agaaatgtat
    36361 gacagatcac tatttttgaa gcctgtcaca ggacgtcatt atagtgtgtg tcagatctcg
    36421 ttttccttaa ccatgttaca tagaatgtgc acggaaattt aacctgcctc atatttggag
    36481 caaatatgga ccgcgtcctt cattttgtac tggcacttgc cgttgttgtg attctcgcac
    36541 tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta
    36601 tcgaagtgtt actggcgtgg ttcttcctga actccgacgt tggtttaggc ttcgtgaaag
    36661 gcttctccga aatgttcgaa aaactgctcg gatttgccaa cgaagggact aacttcgtct
    36721 ttggtagcat gaatgatcaa ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg
    36781 tctttatctc tgcactgatc ggtattctcc agcacattcg cgtgttgccg gtgattatcc
    36841 gcgcaattgg tttcctgctc tccaaagtca acggtatggg caaactggaa tcctttaacg
    36901 ccgtcagctc cctgattctg ggtcagtcag aaaactttat tgcctataaa gatatcctcg
    36961 gcaaaatctc ccgtaatcgt atgtacacca tggctgccac ggcaatgtcc accgtgtcga
    37021 tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg
    37081 tactgaacat gttcagcacc tttatcgtgc tgtcgctgat caatccttac cgtgttgatg
    37141 ccagtgaaga aaacatccag atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc
    37201 tgggtgaata cattctggca ggtttcaaag ttgccattat cgttgccgcg atgctgatcg
    37261 gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc caccgttact ggctggtttg
    37321 gctacagcat ctccttccag ggcatcctgg gctacatctt ctatccgatt gcatgggtga
    37381 tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat catggcgacc aaactggttt
    37441 ccaacgagtt cgttgcgatg atggatctgc agaaaattgc ttccacgctc tctccgcgtg
    37501 ctgaaggcat catctctgtg ttcctagttt ccttcgctaa cttctcttca atcgggatta
    37561 tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc
    37621 tgaaactggt ttacggctct accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg
    37681 tgctgtaaaa ccatacataa aaaagccggg gatcattccc cggctttttt acgcctgtta
    37741 atcagctaat ggctgcgggc gaccaatcaa atacccttgc agatattgca ccccgagctt
    37801 atgcaatagc gcctgctgct gttgcgtctc gacaaactcc gcgaccacac tcaatgactt
    37861 cgctttcgcc agatcggtaa ttgatctcac aatcatcgca tccagcgtat tcgtgacaat
    37921 atctttcaca aagacgccat caattttgat gatatcagcc tgcaaacgct ttaaccgttc
    37981 gtagttggca tatccggtgc caaagtcatc aatcgcaatc cggaaaccaa acttatgcag
    38041 ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac gcctgctcct cggtgatctc
    38101 aagaatgacc gcctgcgggg agatgtgata acgcttaaac agacgaataa tccgcccggc
    38161 aatattcttt tgcagcagcg tgagcggcat taaattgact gaaaagcgcg gtcctttttt
    38221 gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc agcacttgca aatcaaaacg
    38281 cgcgctaagg ttaaactgag caataagggg cagaaactta tccggggtca taatgccgcc
    38341 gtcatatttc agtcgcgcga ggatctcatc ataaccttca ccctctttgt tgcgaattgg
    38401 ctgggcgtag agcagcaaat caccctgatc taacgccgtg cgaatggtat tcagcaatag
    38461 cacctgttta gtggtctgcc cggaaaccat ctcctctctg ctatccagcg ccagcacatg
    38521 atgatgtgcg caggattgct ccgccagcca gcttaactgc cccaacaagg gttgcagggt
    38581 ttcctgattt ccatcaaaac gcccccaggc agcaccatag cccatatcca gcccggtatt
    38641 gttccagtga atttgccgac tattcaggat gttaaccata tgctggagtc gcccttccgt
    38701 ttccggcccg ctcagcacta acagcagttc actacccggc aattgataca acttttcgtt
    38761 ttcctgcatc aacggcagca gcgtacggca aattgagcgg atacagtgaa cgcgcatcat
    38821 taagccgtaa tgacgactca taaactcaag attatcaatg cgcaggcagc aaaaactctt
    38881 gcccgcctct tgctccggcg cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg
    38941 atctgttagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    39001 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcgagaataa atgccagcga
    39061 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    39121 aacagcccag gtgagattta aaaacggata gcgaagatta ccgaccccga gggtaaagat
    39181 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    39241 cagaagtagc agcacgctta acgctgccag ccagcttaag gtaaatgcga gtgagatagt
    39301 agaaaagcat gttgtaaatc agcactgcgg tgaaaaggct tagtaaatca acgaccgtga
    39361 aaatggcatc cgcatcgccg aaaaaggtgg atatctttag cgggaaatca aagaagctcc
    39421 ccacaagata catgctgcat ttgatgccga tcggcgtcac caaaccaagc cagaacagac
    39481 gttgccagac atatcggctg gtcaatccat aacgccaacg cgtccccagc tgccagcgta
    39541 atattgcgca ggcacacaac acgacaaacg tctggctgcc gagcatgacc gcagtctgta
    39601 aaaaagagag atgaaagttc cacagattgg tgcaaaacat cccgcataaa acgggaacga
    39661 caccgcgcca gccaaagata aacaagattg acagcattac gcacagtggc atccacgcca
    39721 gaaagatata actggaatgg ataatggcaa gtggcgaaat aaaacgggat agctgaataa
    39781 gtaccacggt gagcgtaaac gctagtgtga atatcttgat attttttatc aggttatgct
    39841 ccacaaacat gagtacacta ctctcactga tcggggtttt caggcacaca aataataagt
    39901 ttattcgcat cgcgaagcaa acaggggcca gcctgttgcc gatttttcat ccaatgatgc
    39961 tttcaccagg cggcgctaac ccggacgtag actgtattac aaaagcggca aaaagcagag
    40021 acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg tggagctaag cgggatcgaa
    40081 ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccacgatgc
    40141 gtttacgtac caaatttgct gggtgcaaaa tttggtggag ctaagcggga tcgaaccgct
    40201 gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccgt cacgtaaagc
    40261 ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga atgtgtcaac ggcaaattgc
    40321 aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac aattttggaa aaccgctcgc
    40381 attcagtagt tattcatgtc acggtttcct gtaaagtggt gttataaaat gaactactaa
    40441 tagacccaca tacattcagg gaattgttat gttcaaggag aggatgacac cagatgaact
    40501 tgccagactg accggctata gccgccagac cattaataaa tgggtacgca aggaaggctg
    40561 gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc cgactggttc acgtcaatga
    40621 acaggttcgt gaatatattc gcaatgctga acgtccagaa ggtcagggag aagcgcctgc
    40681 cctttctggt gatgcgccgc ttgaagtatt gctggtgaca ctggcgaaag agatgacgcc
    40741 agttgaacaa aaacagttta catccttgct tctgcgggaa gggattatcg gattgttaca
    40801 acgcttaggg attcgcgata gcaaataata tgaaaagatt acgcaataaa atgaccaccg
    40861 aagaactggc tgaatgcctc ggtgtggcta aacaaactgt taaccgttgg accagagaaa
    40921 aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg tcgtgccaga cttattctgg
    40981 tcgatacgca agtttgcgag tttattcaga acacgccagc cttccataac acaccaatgt
    41041 tgatggaagc tgaagagaga attgctgaat acgctcctgg agcacgcgcc ccggcgtatc
    41101 gacaaatcat caacgcgata gataatatga cagacattga gcaggaaaaa gtcgcgcaat
    41161 ttttgtcacg tgaaggaatt cgcaatttcc tcgcccgtct cgatattgac gaatcagcat
    41221 aaataaaaaa cggcaggata ttatctcctg ccgtttatct ttttacacgc taattactgc
    41281 tgattttcgc gttcagcaat aaaatccagc gctttgttga tacgctcgat actgcgggtc
    41341 ttaccaattg cgtgaacggt aacatccagt gctggagact gccccgcacc ggttacggcg
    41401 acacgcagcg gcataccaac tttacccata cccacttcca gctcatccgc cgtcgcctga
    41461 atagcgtgat gaacgttttc agcggtccag tcagtaatcg cggccagttt gtcacgaacc
    41521 acttccagcg gctgacgcgc taccggacgc agatgttttt tcgccgcatc ggcatcgaac
    41581 tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca tctctttcag cgtcttgcag
    41641 cgttcgccca gcagtttcac cagatcagcc agctgcgggc cgttacgggt atcgatattt
    41701 tcctgctcaa tgtgccactg taagtgagta gcaacatact ccggcggcag cgcgttaatg
    41761 tagtgatggt tcagccacag cagcttgtcg gtgttgaacg cactggcaga tttgctgacg
    41821 gcattcaaag tgaagtattt gatcatctct tcacgagtga agatttcctg atcgccgtgg
    41881 gaccagccca gacgcaccag atagttcagc agtgcttctg gcaaataacc gtcatcacga
    41941 tactgcatta cgctgactgc cccgtgacgt ttggacagtt ttttaccgtc atcgccattg
    42001 atcatagaaa cgtgcgcgta aaccggcacc ggcgctttca gggccttaag aatgttgatc
    42061 tggcgcggcg tgttgttgat atggtcttcg ccacggataa cgtgggtgat ttccatatcc
    42121 cagtcatcga caaccacaca gaagttatag gttggggaac catcggtacg gcggataata
    42181 agatcgtcca gttcctggtt gctgaactcg atcggaccac ggatctgatc gtcaaaaaca
    42241 acagaacctt cctgcgggtt agcaaaacgt acaacacacg gttcatcatc agcatgatgc
    42301 tcatggctgt ggcggcagcg accgtcataa cgcggcttct cacctttcgc catttgctct
    42361 tcgcgcagcg cttccaggcg ctctttagag caatagcatt tataagcagt gccctcttcc
    42421 agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt tggtctggta gtacggacct
    42481 tcatcccact ccaggctcag ccagttcatg ccatccataa tggcttcgat agcttccggc
    42541 gtggaacgct caagatcggt gtcttcaata cgcagcacga actcaccgcc gtggttacgt
    42601 gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa cgtgcagata gcctgttggg
    42661 cttggcgcga agcgagtttt gattttcatg aaatggcctt acgtttagaa agatgccgac
    42721 aaccggcaaa tcctggaaaa attgagtggg cgatattcta tcactcccgc ctgattcctc
    42781 aatgtagttc gggtttctac cttacgcatt attgttttcg ttgacaaatt gcgcaacacg
    42841 gtctgttttg cgatcatctt gcttaaattt acgacgaacg aacaatttct ttagaaaatg
    42901 cgttgactca ttttgaactc tccctataat gcgactccac acagcggggg tgattagctc
    42961 agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca
    43021 ccaactactt tatgtagtct ccgccgtgta gcaagaaatt gagaagtggg tgattagctc
    43081 agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgataccg tcatcaccca
    43141 ccacttcggg tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg
    43201 ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc
    43261 tgcgatactc aaagattcga acctgcagca ggtttgagtt gagcgcagcg aaacaacgga
    43321 gccgctcgcg gcgacggccc gaagggcgag cgaagcgagt catcctgcac gacccaccaa
    43381 tgcaaaaaag cgccctaaag gcgctttttt gctatctgcg atactcaaag attcgaacct
    43441 gcagcaggtt tgagttgagc gcagcgaaac aacggagccg ctcgcggcga cggcccgaaa
    43501 ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca aaaaagcgcc ctaaaggcgc
    43561 ttttttgcta tctgcgattt gtaagattga tttttcttct caagttcctt gttcttctac
    43621 cgatattacc tcgtacagtg acgaggactg aacaatggcc actatctctt caacttcaat
    43681 tccttccata caaacacaga gtagtaacag ggcatcacaa ggtagcgatg ttgcttcaca
    43741 aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa caaattaaag agattgtaga
    43801 cactagcggc agtgctgaag acaagcaaaa acaggccgaa ttgattcaac agcaaatcac
    43861 cctactggaa acacaactgg cgcagttgca gaaacagcag gcagaaaagg cgcaggagaa
    43921 agagcagcgt ttgtctctga atgtatccct tctaaatcct gttgaaaaaa cgactcatat
    43981 cgatatctat atctgataca acggatcccc ttcccgacca gccattagcg cattgccctg
    44041 gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt tcttcctgcg
    44101 caccagcatc aactggcgtt caaccaccgg cgtaatgcgc ttaaccacca gtgggctacc
    44161 ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg ccgatgcctg cggctaccat
    44221 cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa taccattgcg
    44281 cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca acaccaattt
    44341 cgctccctgt aatgcctgcc agggtacata atcttccacg gccaaagcgc tatcgcgatg
    44401 gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc ccactgggcc
    44461 tggatcgatg acgatgccaa agtcgacatc tccctggcga atactttcca tcacccactg
    44521 ctgcggacga tcatgcagga caaactgaat atctggatag cggcgatggc tttcagcaat
    44581 gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa cttttccgct
    44641 cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat ccagcagtcg
    44701 ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac gcgtggttct
    44761 gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac ttactgccga
    44821 ctggctcaag ccgatacgct ctcctgcacg actaaaactt ttctcctgcg ctactgtgac
    44881 gaaaaccttt aattgtttta aagaataatt catctgtttt cttcatgaat ggatgcaata
    44941 aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca tctggagtct
    45001 ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg gtggtgttgc
    45061 tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat ctgaccaccg
    45121 cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag gcgattattg
    45181 ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttc gtgctgtttc
    45241 cgattctggg tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg atgctctact
    45301 ccggttttct ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc gccttcacgt
    45361 caatggcggg cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc agcctgctgg
    45421 ggattttcct ttcaccattg ctggttggtc tggtgatgaa tgttcacggt gcaaggggca
    45481 gccttgagca ggtcggtaaa attatgctgc aactgctgct gccgtttgtg ttggggcatc
    45541 tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt gcgaaaactg
    45601 accagacgtc cattctgttg gtggtttata cagcgttcag cgaagccgtc gttaatggta
    45661 tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc tgcgttcttc
    45721 tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgagcttc aataaggcag
    45781 atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc atcccgatgg
    45841 caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcccctg atgattttcc
    45901 atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc cagaccgaac
    45961 agttacaggc gcagcaggaa agcagcgccg ataaagctta aagcggacgc ttcaggggct
    46021 ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt agctcaccga
    46081 gcttccctgc cgggaactca tctttgcggg caaaccacag cagatactcc tccggcaggt
    46141 caattaagcg acgccctttg tatttgccaa acggcattat cgtattggct atttcaatca
    46201 gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc gtcgatgact
    46261 tcaatgccca gttcctgcgc cttcgccagt ttagatcctg cagcttcacc cgctatcacc
    46321 agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac cagtcgagct
    46381 ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt tttaccagca
    46441 aacgggctgt caatctcttc cgcgttgata acgatcggcg caggccagtg aacaccttcc
    46501 gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt gtgaacgtgg
    46561 gatgcaacga caatgccaac atcaggcacc ttttgcagct cttcaatcga agcggcttcc
    46621 agcgcttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc gccgacttca
    46681 cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt ttccagcgcg
    46741 ttaaccacgt tttgtgccaa ttttggcccc atacgctcca gtccggtcag tttgcctgcg
    46801 gtgagtttga acagatctgc cggagtatgg acatattctt tttcaaccag ctgatcgatg
    46861 attttgtcgc ccattccgtc aacatccatc gcacggcggg aaacaaagtg tttcagcgac
    46921 tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc ttcaccttcc
    46981 acacgctcaa cgtcagaacc acataccgga caatgcgtcg ggaatacaac ctcacgggta
    47041 tcttccgggc gttcagaaag cacgacgtta accacctgcg ggatcacgtc gccagcgcgg
    47101 cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc cgcattgtgt
    47161 aaggttgcgt tactcaccag cacgccagca acatggacag gttccagacg cgcaacaggc
    47221 gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt catctgctcc
    47281 tgcgccggga atttaaacgc aaccgcccag cgcggggcac gcgcgacaaa gccaagctgc
    47341 tcctgctgtt ccagtgagtt gaccttaatc accacgccgt cgatatcaaa gcccagcgtc
    47401 gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga ttcacaaagc
    47461 gttacccgat cgctgaccgg caccccccac tttttaaatt gcagtaaacg gccaagatga
    47521 gtatccggca gctcgccacc ttccagaaca ccaacgccat agcagaaaaa agtgagcggt
    47581 cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc attacgtggg
    47641 ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc gaaccccgcc
    47701 tgcggcagga acacttcacc acgcacttcc agacgcgccg ggatattctc tccgtgcagc
    47761 ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc ggtggtgcca
    47821 tcgccacggg tcgcggcact gactaaaacg ccattttcat acagaatact gacggcaagg
    47881 ccatccagct tcagctcaca gcaccaggtg actttctcgt tgtttttcag acggtcctgc
    47941 acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa cgttatccag tgacagcatt
    48001 ggtacttcat ggcgtatctg gctgaaagct gccagcggcg cagcacctac acgttgggta
    48061 ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag ttcgcgcatc
    48121 agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata aagatattca
    48181 tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc catatcgcac
    48241 catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt tgccttaagt
    48301 gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc gcaatttctg
    48361 cggagtcatc atacggcgct gatcgtcaag cacgacaccg cccacttcat cggcaatatg
    48421 ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag acggtacctg
    48481 cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa aggttcccgg
    48541 tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc taagatgacg
    48601 atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt taagaagcag
    48661 ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca ccgcttcttt
    48721 gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct gtggtgccgc
    48781 tacgggttct gcaggctgga aagcctgttg tgccggttgc ggtgctgaat gcacaggctg
    48841 cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag gctgcggctg
    48901 ataggcaggc tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc
    48961 ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gtaggtactg
    49021 gtgttgcggc gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac
    49081 gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc
    49141 gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc
    49201 tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat
    49261 gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca
    49321 ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta
    49381 ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat
    49441 cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca
    49501 aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta
    49561 ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt
    49621 accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct
    49681 cgatgtctgg atcccgactt tcatgagtta cgttccggac tggctacaat ggctgagtta
    49741 tctgttgtgg cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac
    49801 gattgctaac tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg
    49861 attgactggc gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg
    49921 aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa ttgtattact
    49981 aattctttac ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt
    50041 tagcgcctgg atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc
    50101 gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc
    50161 tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt
    50221 ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta
    50281 acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc
    50341 ttattccatc ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt
    50401 ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac
    50461 aggccatgag taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc
    50521 gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct
    50581 tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc
    50641 tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct
    50701 atgtagctgc cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg
    50761 aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag
    50821 gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc
    50881 tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc
    50941 cggaaatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg
    51001 gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta
    51061 tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggtgctg gcaggtgaag
    51121 agattaaacc tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc
    51181 tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg
    51241 cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg
    51301 ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc
    51361 taccatcatc gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga
    51421 aagaattgca acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg
    51481 cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac
    51541 tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa
    51601 agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat gcttaatgac
    51661 gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt
    51721 cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact
    51781 ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac
    51841 cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa
    51901 gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct
    51961 gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg
    52021 cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt
    52081 tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta
    52141 gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt
    52201 gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt
    52261 cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt
    52321 gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag
    52381 caggaaatca tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa
    52441 gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt
    52501 gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt
    52561 atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc
    52621 gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt
    52681 acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt
    52741 agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag
    52801 gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg
    52861 gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt
    52921 caccaggtag aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt
    52981 aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg
    53041 ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg
    53101 caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac
    53161 ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt
    53221 aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg
    53281 cgcattatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc
    53341 gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc
    53401 ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt
    53461 gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt
    53521 aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag
    53581 caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct
    53641 ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc
    53701 gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag
    53761 gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac
    53821 aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta
    53881 ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg acaaaaagga
    53941 taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt
    54001 gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg
    54061 taacaaaatg gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc
    54121 gttctctatc gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt
    54181 tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg
    54241 cgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac
    54301 tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt
    54361 aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat
    54421 ggcgcccatc ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct
    54481 ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc
    54541 atcgtttaac gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc
    54601 agtccctttc aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact
    54661 gaccaccacc acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac
    54721 ccattttaat gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg
    54781 gcaattttta ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag
    54841 cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc
    54901 aatatcgcca atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc
    54961 agtcagccac tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac
    55021 agcacgaagt tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca
    55081 ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac
    55141 cgtcggcacg gcaaagacat tcaagccgtt ctgttttata gcaggcaccg caatgctgtt
    55201 gcctacgctt ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct
    55261 actcttatcg ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc
    55321 tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac
    55381 aaagataagg taattcagtg aagaaaatca tttgtctggc cattacgctg ctaatgacac
    55441 tccccgctta cgcgaagtta actgttcatg aagaagcccg catcaacgcc atgctggagg
    55501 gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg
    55561 aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg
    55621 agcagtttat tgataaggta gcttcgtcgt catcgattac cgggaagccg tatatcgtga
    55681 agatccccgg taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga
    55741 cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat ttacaggagg
    55801 taacatgaaa aaatgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga
    55861 ttacgaaggg gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca
    55921 tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc
    55981 aaaaatgaaa aaaggatatc aagaaactcc agactttaac ttcatggatc tctactattt
    56041 tgatgatgaa gagattgggt tacatcttaa aacgtcacac ccaaacttcc agtgccattt
    56101 tactgatcca ctttatatgt gttgctggga tgaagaatct ccttttggta gcgatgaagg
    56161 tgctgatgct ctaaacgaac ttgaaaatag cctccgttct ggactgtgct gatttccctc
    56221 aaatgttaat tgaaactatg tggggtatga aatacatcgc tatggacagt attcttgaag
    56281 aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc tatccagagc aatatgatta
    56341 cctacgcgac tgcattcggt cagatcaaag tcatgggtaa aatctcccat aaacttaaaa
    56401 agatgggact caatgcacta gcgcgtcatc agcttaccgc aaaaattctt caatggggtg
    56461 acggtcagga ctcaccaata cttcaaaaaa tgattgatga ccttactgct tttccacacg
    56521 aaaattaaaa actgcatttg tcggcagcaa caactgccga caaatcagat tattgacaaa
    56581 gtgcactttg tttatgccgg atgcggcgta aacgcctgat ccggcataaa aatattgcta
    56641 attcaatata ttgcagaaac attgtaggcc tgataagcgg agcgcatcag gcagttttgc
    56701 gtttgtcatc agtctccaat gctattaatc cttaaatccc tgccccctga ctaaaatgct
    56761 cttccccaaa cactccggtg gagaggtagc gatcgccacg atcgcagatg atcgctacca
    56821 ccaccgcacc agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgcctgagc
    56881 tgacgccaca gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc
    56941 gctgatgaat atccagcacc tcatccacca gcgcagcgtt gaaaatcccc ggcagatatt
    57001 ccgcaggcca gcggcgaatg cccggaatac tgctgccttc ttccggttgc aggccaacaa
    57061 tggtcaccgg tttggattgt tcgcgcataa agcatgaaac gccggtgata gtgccggtcg
    57121 tccccatgct ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag atttccggcc
    57181 cggtggtggt gtaatgcgca taagggttat cgggattatt gaactgatcg agcagctttc
    57241 cttcgccacg attcgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt
    57301 tggtgacaag aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca
    57361 tgttgtcggg catcagcaat ttcatgcgat agcctttcag cgcggcaatc attgccagcg
    57421 caatgccggt gttaccactg gtggcttcga ttaagacatc acccggttta atttccccgc
    57481 gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa cctgccgggt
    57541 tattgccttc cagttttaac cacacttcac tgccgttatc cggccccatt cgctgcaact
    57601 tcaccagagg cgtattgcct attgtttgtt ctaatgtact cacaatctct atccatacgt
    57661 ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca
    57721 ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag
    57781 gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac
    57841 agccgcgcat gttgcagacc aacgaataaa cgctcgccat gctgcggggc atcgtcgcca
    57901 tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg
    57961 tagtgacctt tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gagactggta
    58021 cggcggctga tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca
    58081 ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt
    58141 ccctgcaggc ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc
    58201 catacctgat tcggcgcatc agcctgttca atattcccct gactcatcac cactacacga
    58261 tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt gaatttcagc
    58321 tcttcatgca gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg
    58381 ccgaacggtt catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagcgcc
    58441 acacgctgtt tctggccgcc ggaaagctgc gccggataac gatccgccag atgcgcaagc
    58501 tggaccattt ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg
    58561 cgacgcggca gcaccgtcag gccaaaagtg atattgtcga ataccgtcat atggcggaac
    58621 agcgcgtaat gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag gcgactcacg
    58681 tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatccgc
    58741 agcagcgtgg ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga
    58801 atatccagtg agatatcgtt cagcacctgg gtgcgaccaa aagacttctt gatattggca
    58861 atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca
    58921 ctgcaacata ctctttaaaa acagggtgat aatcgccatc aacgtcaaca gcgcggcggc
    58981 ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta acggcagcga
    59041 aagtgtttcg ccgcgaatcg agccggaaac caccgacact gcaccaaact cgccaatcgc
    59101 gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag cgaatattcg gcaatgtgac
    59161 gcgacggaac atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt cttcctggct
    59221 gccctggctt aacatcaccg gcaccagctc gcgcaccaca aacggacacg tcacaaagat
    59281 ggtgaccagc accattccgg gccaggagaa cataatttgc agattatgtt catccagcca
    59341 gccgccgagc gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg
    59401 cgatacagca aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg ggaagttaaa
    59461 gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag
    59521 cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa
    59581 aaccggcatc agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag
    59641 gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg
    59701 gcgcgcgtca taacgcttca attgggtaac ttccgccatt aatgacctac cacacgccga
    59761 ccaaagcgac tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc
    59821 accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac aaaaatcatc
    59881 agcgacgtca cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat
    59941 tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc
    60001 ggcagcacca ctttgcagaa actctgccag cgcgttgcac caagcgtttc cgccgcttct
    60061 tcatattccg ggcctaactc ttccagcacc ggctgcacgg tacgcaccac aaacggaatg
    60121 ctggtaaagg ccatcgccac cgcaatacca agccaggtat aggtgacttt gatatcaaac
    60181 ttcgccagcc attcaccgta aaaaccgttt acggaaaaga gcgaggccag cgttaaaccg
    60241 gcgactgccg ttggcagcgc aaagggtgaa tccatcagcg catcaagcag cgtgcggccc
    60301 ggaaagcgat aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc gttaaaaatc
    60361 gatgccacaa acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg
    60421 atcacctccc agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcagagagc
    60481 ggcagcagca atatcaggca cacaaacagc agactggtgc caaggcttaa agtaaagccc
    60541 ggcagcacgc gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt
    60601 ctaactcgcc gccgctggtg aagtgggttt tcatcacttc cggccatgag ccaaatttgt
    60661 cttccacgcg gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg
    60721 ggttattcac acggtagtaa tagtcggtga tgatggtctg cgcctgtggg ctgtagagcc
    60781 agttcaggta ggcttttgcg gctttttccg taccgttggc ctgcacattt ttatcgaccc
    60841 acgccaccgg gaattccgcc agaatattgg ttttcgggat caccacttca aagccctgtg
    60901 cttcatactg tttacggatg ttgttcactt ccgattcaaa gctaatcagc acatcgccca
    60961 agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac acttcaacgt
    61021 ttttcaggaa ctgggtcata aactgttcgg ttttggcttt atcgccaccg tcagctttat
    61081 ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg
    61141 ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt
    61201 tgcccttacg caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc
    61261 gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg caggatttgt acgtcggtca
    61321 cctggttata agtgacaacg tctgctttta agccctgcaa aatcgccagc gcctgttttg
    61381 atgacccggc atgagattgt tttatcgtca gtttgtcgcc gccgttatct tttgcccatt
    61441 gctgttcaaa cggcggattc agggcggcaa acagctcgcg ggagacgtca taagaactgt
    61501 tcagcagttc cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg
    61561 agttcttttt cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa
    61621 tagccatcat atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat
    61681 ttggaagtta aaaagggaat aagaacttgg ttcaacaggc gtaattgttg caggcagttt
    61741 ggacacggac agcgcggaga aaccggagcg tacacatagt acgtgaggat ttcgagcact
    61801 gcccggggcc aaaatgacaa atgaaatagc ctggtgaact tagttcaaga cgaaatcctc
    61861 cccacacatg cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca
    61921 gtgtgctgcc gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg
    61981 cgaggaaggc cgccagttcg ccgacttcca gcggatcggc gaggcgacac agcgggattg
    62041 cttttgccat ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc
    62101 tttccgccat tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga ataccggatt
    62161 gcgcgtactc caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat
    62221 acgccgtttc gccaggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc
    62281 gaccatcttt gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt
    62341 taatattgat atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca
    62401 gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg cccttctttt tccttcgcgc
    62461 gtttgatagc tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc
    62521 gatgaccacg accacacagt tcgtccgcta gcttttcgat ctcaggggag atatccagca
    62581 agattaggtt cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca attccctgca
    62641 atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt
    62701 taatacagag cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc
    62761 ggatcagctc actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag
    62821 gcgttgtgtt agctcactgc tgcgctgaat cattttcaat gacgccacat cattgatgtt
    62881 gcacaagacc aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg gtagacatcg
    62941 acgagctacg ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc gccagccgcc
    63001 gcagcggaga gtcagccaga gaagtaatgg caatcaccgt tgccccctgc tttcgtgccg
    63061 cttctgcgca caagacaatc tcttttttac tgccgctgta agagatagcg atttgtacat
    63121 cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct tcgcaggcca
    63181 cgcgatagcc aattttcatc agtttgaacg acagatcgcg ccccaccagc gccgatccgc
    63241 ccaggcccgt tatctggata aacggtgctt tactgatgac ctcaatgatt ttttgcagcc
    63301 gcgcgtagtc gaacaacgca caggtttgct ccagcgccag ctctttttcg cgattcagtt
    63361 tgcgggcaat cacttccagc gaatcatcac tggtaatcga actgtgcagg tgcagcgccg
    63421 tggcattcgt tttctcccgg ctggcactgt attcgccaat taacgccata cgcaattcag
    63481 taaatccttg cgcgccgagc ttctgggcaa atttcacaat gctcgactgg ctgataccga
    63541 gctgtttcgc catctgccgt gaagagacgg atttcagctc gctcactctg gcccgcagaa
    63601 agtcagcaat tttttgctcg ttcccggtaa attcactttc cgcattgcgt attttcgtca
    63661 ggtacaacat cttccactct cctgggctgg cagcaaaaat tccgcttaag ttcttatgac
    63721 gctcttcaca ctctgcgagt gtagactcaa tgattccttt aagtctgtca attcgggata
    63781 tatgattcca tacatctgca attatttgat cgtaaatagt aaggtcacca ccgatgcaac
    63841 tagaaaagat gattactgaa ggctcgaaca ccgcctcggc tgaaattgac cgcgtatcga
    63901 cgctggaaat gtgccggatt atcaacgatg aagataaaac cgtaccgcct gccgttgatc
    63961 gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc agcggcggcg
    64021 gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct ggggattctg gatgccagcg
    64081 aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt tggtttgatt gctggaggcg
    64141 aatatgccat tcagcacgcg gtggaaggag ctgaagatag ccgggaaggt ggtgttaacg
    64201 atctgaaaaa tattaattta acggcacagg atgtggtggt tggcattgct gccagcggtc
    64261 gcacgccgta tgtgattgcc ggactggaat acgcacgcca gctcggctgc cgcacagtgg
    64321 gaatttcctg taatccgggg agcgccgttt caaccaccgc tgagtttgcc attacaccga
    64381 ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa agcaggtaca gcgcagaaac
    64441 tggtgctcaa tatgctttcc accgggctga tgattaaatc cggcaaagtg ttcggcaacc
    64501 tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc aatatcgtta
    64561 aaaacgccac cggatgtaat gccgaacaag cggaagcggc gttaattgcc tgcgagcgca
    64621 actgtaaaac ggccattgtg atggtgctga aaaatctcga tgccgcagaa gctaaaaaac
    64681 gcctggatca acacggcggc tttattcgtc aggttttaga caaggaataa cccatggcca
    64741 aagagatcag cagtgaactt ctgaacacca ttcttacccg tgtcggcgga ccgggaaata
    64801 tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac agttcactgg
    64861 ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc agcgaccagg
    64921 tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg agcgagctgc
    64981 tgggcgatgc accggtacag gatgccgcag aaatcgccgc ccagaacaaa cgtcagttaa
    65041 aagccaaaca aacctccggc gtgcaacaat ttctcgccaa attcgccacc atcttcacgc
    65101 cgctgatccc cggttttatt gccgccggtc tgctgctggg gatagcgacg ttaattgcca
    65161 cggtgatgca cgttccggca gatgctcagg gcacgctacc cgacgcgctg aattttatga
    65221 aggtgttcag caaaggtttg ttcaccttcc tagtgattct ggtgggctat aacgccgccc
    65281 aggcatttgg cggcacgggc gtcaatggcg caattattgc cgcactcttt ttgctcggtt
    65341 ataaccccgc tgcaaccacc ggttactacg ccggttttca cgatttcttt ggtctgccca
    65401 tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc gtgggcctgc gcccgcattg
    65461 aaggcatggt gcgccgcttt atgccggacg atctcgacat gctgctgacc tcgttaatca
    65521 ccctgctgat caccgctacg cttgcgtacc tgatcattat gccgctgggt ggctggttat
    65581 tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa tccgttcggt tgtgcggttt
    65641 tagccgggct gttcctgatc gccgtggtat ttggcgtgca tcagggcttt attcctgttt
    65701 acctcgcgtt aatggacagc cagggattca acagcttatt tcccatcctt tcaatggcag
    65761 gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg cacagtgcgc
    65821 tacgcagtca ggtacgcggg gcgattattc ccggcctgct gggcgttggt gaaccgctga
    65881 tttacggtgt caccctgccc cgcatgaagc cgtttgttac cgcctgttta ggcggcgcgg
    65941 cgggcggttt gtttatcgga ttgatagcct ggtggggtct gccgatgggc ttaaacagcg
    66001 cctttgggcc gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa ggcatccttc
    66061 cggcaatggc ggtttatgct ggcgggattc tggtggcatg ggtttgcggg tttattttca
    66121 ccacgctctt tggctgccgt aacgtcaatc tggactgata ttatgaaacg gacaatgctc
    66181 tatctttctc tgctggctgt tagttgtagc gtcagcgccg caaaataccc tgttctgaca
    66241 gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc agtcagtaat
    66301 gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca
    66361 gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac
    66421 gtttgccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg
    66481 cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct
    66541 ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg
    66601 tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca
    66661 tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca
    66721 caaacacata gctgccgccg gaatccacgc cgtctttgat aaccgccact tcgcggcgcg
    66781 tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca
    66841 cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg
    66901 ccgcctgagc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat
    66961 cgaactgggt cgtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg
    67021 ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa
    67081 gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg
    67141 ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc
    67201 aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg
    67261 ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga cggcggtttg
    67321 ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc
    67381 gtccgaaggc attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg
    67441 ttgactgcct tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca
    67501 attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc
    67561 agcaggaata tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat
    67621 caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc
    67681 ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc
    67741 ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggacga
    67801 tccaaaccag ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc
    67861 ggtcagcatc aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt
    67921 ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc
    67981 ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc
    68041 cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg
    68101 ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaggt
    68161 gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaataccggg
    68221 aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc
    68281 ctgctcacgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag
    68341 cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg
    68401 gaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt
    68461 catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct
    68521 gaattttcgg gcagccgcga gcaatcagct ttttctccag ccggttaagc aacgcattgg
    68581 caattccacg cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc
    68641 cgtcataacc gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca
    68701 aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca
    68761 gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc
    68821 gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg
    68881 tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca
    68941 actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga
    69001 tacgatataa catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc
    69061 actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg ccctgacgtt
    69121 gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca acggacacag
    69181 caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg
    69241 cggaattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc
    69301 gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg
    69361 ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc
    69421 agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc
    69481 ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg
    69541 cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca
    69601 gcaagtactg tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc
    69661 gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc
    69721 cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat
    69781 caccaacccg gaagaagagc ggctgttagg cacggcggcg tttcgtcaga aaatcgccac
    69841 agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc
    69901 gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct
    69961 tcaggatacg atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag
    70021 ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg gtggtgtttt
    70081 cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg cttccgccac
    70141 cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt
    70201 gcatccgcat aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga
    70261 aaaaccgggt gcagagcccg tttggtggtt tggcggcggc ttcgatttaa cccctttcta
    70321 tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gccagccatt
    70381 tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca
    70441 tcgcaacgaa cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt
    70501 cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc
    70561 aattgtagag cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta
    70621 ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct
    70681 gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga
    70741 gtatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt
    70801 cagggattgg gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga
    70861 ctgcttctcg gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc
    70921 ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg
    70981 gcgtacggcg ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc
    71041 gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt tgattacaca aatccagcac
    71101 cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg
    71161 gtaactctga tgactgatgc tttccgccgt caccattggt tgcgcttctt ccagcatcgc
    71221 ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt
    71281 ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt
    71341 atgctgctct ttcacttcca gcgccgactg gctacgcagc atatgtaata cccgatccgg
    71401 attgtgcaaa aagttagcct gacaggtgat gacatcttca gacagcacta cgcccaggat
    71461 cgtataatcg tccggcgtac ttaattcaaa ctcggttcca ccggggcgcg tcgctatttc
    71521 tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc
    71581 aaaccagaac gagttcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc
    71641 acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg
    71701 atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc aaagcatgtt catagacatc
    71761 cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc
    71821 gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt
    71881 aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata
    71941 acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc
    72001 tttttccagc ggccagccaa agtgggcggc cacttctcct gccgtcattc cctgacatac
    72061 tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg
    72121 ttcctttacg ggttgcttcg gctggcggtt aaagccagta accagccact gggtgtcatc
    72181 gtctggccga ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca
    72241 acctgcgtcc agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaagcg
    72301 accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc
    72361 cgctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat
    72421 cggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt
    72481 aaaggcgtta caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta
    72541 gttggtttca gacggtggcg ggacgtaggt cccagctgta cgtcggcaga tttcaacgcg
    72601 gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca
    72661 agcgtgatcc cggcggttga tgaaagataa gaaccggtac gcgaaaccac atgcgccagg
    72721 aaggcggtgt tttctgcgtc attggcccac tggaaagctg cgccattttc aatatgtgca
    72781 accatcgcat ccagaccggc acgcacttcc gccgggtttg gcccaccaag cataatcagc
    72841 acttcaccgg cagtcggtga cgggccgtgt gccgctcccg catacagcga gcgaccatat
    72901 accacttcga ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt tacgtcgtca
    72961 gaatcggcag aaatcagccc gaggctacga atatgcggcg gcaattttag ttcacgcgca
    73021 aattcagcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg aatcaaatct
    73081 aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc
    73141 atacgtttcg ccaaatccac aatgacggca cagcagcacg aaagagaagc aaaagtcagc
    73201 tacctggtac actaggcagc gggtcagcaa aggggggcaa atgccccttt tttcatgtca
    73261 gataatcaga tcattacctc aataaactaa taatcacatc gcggttacaa ccagtttgat
    73321 agtgatagct caaacccaga cgtggcgctg gctgcaagca aaataagccg caaaaatgcg
    73381 accccatgtt tactagccgc aaaaatgcgg tcctatttgc cagaaccttt tattattcaa
    73441 ttggttagtg caagaaaaag cgtgattact gttcaagaag tatccagaaa ggtcatgatt
    73501 ttgacgctga aaaaaaatag gtgggcattt ttgcccactc actagccgca aatttgccca
    73561 ctcactagcc gcatttttgc ggcccaaagt aataaataca gagaaataat aaatacagat
    73621 cagcgccaag atttttcgtc ttttcggcgg aatcatgaga ctagtgctta cgtcctgata
    73681 acctccagag cagaccttaa cttctgtgca gcaggactac gctcattgcc gctcggctag
    73741 gtcaaatcct cacgataaag ctgttcggat tgtgaccata atgttaaagg actctttaaa
    73801 tcattccttg acgaattaac tagctggctt aagcggaata gcatgcagga taaaaacaaa
    73861 accagcttag cagtggggag agcttcgctt cgctcgtctc ctggggccaa tgctcaatga
    73921 aacgacgtaa ggcacggagc gctcaccata ggtagacaca taaacatctg agagcgcacc
    73981 ttctatcgcg ctacgcgctc aataaaaacc aacgacaccg ccacactgca agaccaatgg
    74041 tttttatcgc gtatacagac cagacaggaa acagcagcaa agcagcgtat agccaggtgc
    74101 agacagcaca ctcacacgac gcacagcagg cttaagtgat cactcaggaa actttatccc
    74161 cgccgccgat caccgcgaac tatcatgacc gcgccaaatt cgaaaggaat ggcccataga
    74221 acgcgatgaa cacacataag cgctacactt acttaacgtc gccacagagc gattgagagc
    74281 gcttaaaatc gatgcgattt cattaaattt ttctcactcg cctgaatggt ggcgatcagg
    74341 actgatcgca acattgcccc gcctcgatat tgagcgcata atagatacat acattaagcg
    74401 gaaaagagat aagtttattg tgaaaatcag ggggttacat atcatgcgca tatcagaaca
    74461 acaacggcag atcctcgact atctggcaaa aaacgaatca atcaccgcac gacaagccgc
    74521 agatctcgtt tatggtggca atgttacccg cgtccaggtt gacgccgccc gccgctcact
    74581 catgacgatg gttagtaatg ggctaatcag gaaacaaggc cgcgctttcg tagctgaaag
    74641 acatatcaat cacgcttata gccaggacgt tgaagaattg cgccgccaga tcctttgggt
    74701 attcagcaaa cagggtcagg agtatttcct ttctgaacac ccaaatttcg ccgccaaaga
    74761 gttaacagta agcggattgt cgtgcgcact ggattatctt gtcggtgaag gttgcgttac
    74821 ttacaagcag ctatggcacg ccctgaatac gcttgtagaa gatggtttac tcattgccac
    74881 gcgtgagaac atcgaatact ggataatgcc gcgttcaggt cgccgcaatc atgcacggta
    74941 tatttcaggc aacagataca gaacgccgga ctttaatccg acacaggaag atcgggaccg
    75001 tgccgaaatg atgtgcaggg aacaggagaa aagaattatg gataagttat tttcgatgca
    75061 gtgaacacaa gcgggcgcaa tgcccgtttt tattgcctta ttccacgcct gacgaatcaa
    75121 tttgatcaca cctgagaaaa tgcatagcca gaagttaagg ccgttatatt gcgattggga
    75181 gcgttttaaa cggtatcaat ttaattaatt ctgatgtggt gatttgcagg caaaaaaaac
    75241 ccgccggaga agcgggaaat agagacatat cgttgtagtt attaacagat cactttgagg
    75301 tcgagggcgg ctaaccctcg ctaatattat cccgctatta ccttgcgggt gcaaataatc
    75361 ttttattgct ttgatgattt aataaatagc actttttgct aacaaaaact gctgtcaata
    75421 tatggccaaa aatgtcaaca gaaaaaataa ggtttataat attaagtatc acatgggtaa
    75481 tgggttcagg gaatcggaat ccaccgcctc acggaaattc aaaatttgct tcttctttcc
    75541 tccgtgggcg gcttcttttt cgtatcgagg tatttatgaa agcaataaca ttagaacagt
    75601 tcgcagcaat ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa aaaccatcaa
    75661 ccggagtccc tttgccattt accccacgcg gtactcgcca attattctgg gagcacgaat
    75721 gcaaagaatg gttgtacaga acagaacgat aataatcacg catcacattt tgaggtaaac
    75781 atcatgacca atatcgaaag attagaacgc tgtgccgata ttatgcggcg cagatggatt
    75841 tatgaccctg aagcgggttt attgtttagc cgtgaaacaa aagacgttat caaaggcagc
    75901 atgaccaata aagggcattt ggttgtaacg gtacatgaag gtaaattcaa agtgacactc
    75961 acctatcaga aagcctgcta tgtatacaca tacggcgctt atgacgagac attatatgaa
    76021 gtgcaccacg ttaatttgaa caaacaggat aaccgcatca agaatattcg cctgatgccg
    76081 aaagaaaagc accgccggat acataaaaac gtccgcaaac ttattcggct gggccacaca
    76141 caatggcttc aaccgcaatt aattgaacaa tcactataaa cacaggagta aataccatgc
    76201 attcattaga cagttatttt cagcgcacga cagcccctaa atcagccgcc caggaacgcc
    76261 gtgaggaatt ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg
    76321 aaacggttcg tccacttgtt gatgaagtag ctgataaact tcagtctgaa atgccggaag
    76381 atatggaagg cactgcaaag cggcgactca tttgcgaatt aagtcgtcgt tttggcgtgt
    76441 caataagcgc ttttaaataa tcgaaaaaag gggggaaaga taatggacgc atttgaggta
    76501 ctggcaaata aaggcgctga actggtagca cagcgtgata aaaccgccaa cgaaggggaa
    76561 cgcgcactgc taaataaaca gattaaagcg atccgtatgg cccagtttaa attaatcagt
    76621 aatgaagtta tcgaaacaat taaaccgcag atcccacaaa tcgttgctga cgctattaaa
    76681 gccgccggac tggagaaacg cattgccggg ctaaaaacga acgcagagaa aatggagatg
    76741 cacaaacaga gcggtgctcc gaatcccgat gagtacttct caccggatgt tgagtttatg
    76801 gcgcaggttg aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg ctattttgat
    76861 ggagtggata gttcggcagg tattgacctg aactcgtatt tcggtcgtga aattgagcat
    76921 tttgatgccg ccccgtctat gccggagcca gaaccagaaa tggccaccga tcctgaagct
    76981 gagcaacgga aacgcgtatt caatgcgata gggattcggt actgatcgga taaaagaaag
    77041 ccgggaatta ccccggctga cttaacaatg tgaccaatga acaaatcgga caagacaaac
    77101 aggataagca gtctcgaaac aatgttcggg aactatatta gcacaagtcc ggagaaaatc
    77161 atcatgttgg cactacgcat catgcagggc atcgctaaga ccctcgcaga acatgtctta
    77221 gacctgaaac attcgccact gagcaaacag gccatgaagc gccaaacgct tcgtctgtgg
    77281 gctgaatatt cgcttggaac tatcaataaa atcatcgaca tgaaatcggg gccgagcaac
    77341 cagagcgcgg aggaaatgga gttcatcagg cgtttaattc tcattcgcag agacattcat
    77401 agccagttac acagcgtggg aatcgatatt aatgatggga cgggcgatta attttcggac
    77461 aagtactatg cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac aggctggaga
    77521 tactcgaagg ccagcgggca gaagcgaaac aactacgtaa gcaggccaga agagcgcaca
    77581 ggaacaacga agcagagtta ctaaccaaat acatcagttt caccaattac tgcatctacg
    77641 agtgttacaa ggaagatgca gaggactggc tggattcatt gccagaacag tactgagcag
    77701 aataacacca cgtcatgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat
    77761 ttagtgtgcc acttcagcat caccgttgaa atcacaataa cgattttttt gtaaatatgc
    77821 tctcacatat gtaggaatag accaggttgg cattgaaaca catctaccat gaccataata
    77881 aa
//
LOCUS       DAEBXD010000004        72314 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEBXD010000004 DAEBXD010000000
VERSION     DAEBXD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72314
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.004"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..85
                     /locus_tag="KJL94_000261"
     CDS             <1..85
                     /locus_tag="KJL94_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4408766.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            133..1254
                     /gene="roxA"
                     /locus_tag="KJL94_000262"
                     /gene_synonym="ycfD"
     CDS             133..1254
                     /gene="roxA"
                     /locus_tag="KJL94_000262"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBD4408767.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(1303..2529)
                     /gene="pepT"
                     /locus_tag="KJL94_000263"
     CDS             complement(1303..2529)
                     /gene="pepT"
                     /locus_tag="KJL94_000263"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBD4408768.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            2779..3915
                     /gene="potA"
                     /locus_tag="KJL94_000264"
     CDS             2779..3915
                     /gene="potA"
                     /locus_tag="KJL94_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBD4408769.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3899..4756
                     /gene="potB"
                     /locus_tag="KJL94_000265"
     CDS             3899..4756
                     /gene="potB"
                     /locus_tag="KJL94_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBD4408770.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            4753..5547
                     /gene="potC"
                     /locus_tag="KJL94_000266"
     CDS             4753..5547
                     /gene="potC"
                     /locus_tag="KJL94_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBD4408771.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            5544..6590
                     /gene="potD"
                     /locus_tag="KJL94_000267"
     CDS             5544..6590
                     /gene="potD"
                     /locus_tag="KJL94_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBD4408772.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(6746..7567)
                     /gene="cobB"
                     /locus_tag="KJL94_000268"
     CDS             complement(6746..7567)
                     /gene="cobB"
                     /locus_tag="KJL94_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBD4408773.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(7583..8494)
                     /gene="nagK"
                     /locus_tag="KJL94_000269"
     CDS             complement(7583..8494)
                     /gene="nagK"
                     /locus_tag="KJL94_000269"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBD4408774.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8523..9767)
                     /gene="lolE"
                     /locus_tag="KJL94_000270"
     CDS             complement(8523..9767)
                     /gene="lolE"
                     /locus_tag="KJL94_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBD4408775.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9767..10468)
                     /gene="lolD"
                     /locus_tag="KJL94_000271"
     CDS             complement(9767..10468)
                     /gene="lolD"
                     /locus_tag="KJL94_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBD4408776.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10461..11660)
                     /gene="lolC"
                     /locus_tag="KJL94_000272"
     CDS             complement(10461..11660)
                     /gene="lolC"
                     /locus_tag="KJL94_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBD4408777.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11922..12995
                     /locus_tag="KJL94_000273"
     CDS             11922..12995
                     /locus_tag="KJL94_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBD4408778.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            13123..16569
                     /gene="mfd"
                     /locus_tag="KJL94_000274"
     CDS             13123..16569
                     /gene="mfd"
                     /locus_tag="KJL94_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBD4408779.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16712..17674
                     /gene="ldtC"
                     /locus_tag="KJL94_000275"
     CDS             16712..17674
                     /gene="ldtC"
                     /locus_tag="KJL94_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBD4408780.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17757..17969)
                     /locus_tag="KJL94_000276"
     CDS             complement(17757..17969)
                     /locus_tag="KJL94_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4408781.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18254..18886
                     /gene="comR"
                     /locus_tag="KJL94_000277"
     CDS             18254..18886
                     /gene="comR"
                     /locus_tag="KJL94_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBD4408782.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(18948..19487)
                     /locus_tag="KJL94_000278"
     CDS             complement(18948..19487)
                     /locus_tag="KJL94_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD4408783.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(19714..21018)
                     /gene="ndh"
                     /locus_tag="KJL94_000279"
     CDS             complement(19714..21018)
                     /gene="ndh"
                     /locus_tag="KJL94_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBD4408784.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(21426..21968)
                     /locus_tag="KJL94_000280"
     CDS             complement(21426..21968)
                     /locus_tag="KJL94_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4408785.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(21991..23016)
                     /gene="nagZ"
                     /locus_tag="KJL94_000281"
     CDS             complement(21991..23016)
                     /gene="nagZ"
                     /locus_tag="KJL94_000281"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBD4408786.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(23027..23851)
                     /gene="thiK"
                     /locus_tag="KJL94_000282"
     CDS             complement(23027..23851)
                     /gene="thiK"
                     /locus_tag="KJL94_000282"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBD4408787.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(23832..24473)
                     /gene="lpoB"
                     /locus_tag="KJL94_000283"
     CDS             complement(23832..24473)
                     /gene="lpoB"
                     /locus_tag="KJL94_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBD4408788.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(24488..24865)
                     /locus_tag="KJL94_000284"
     CDS             complement(24488..24865)
                     /locus_tag="KJL94_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBD4408789.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(24868..25227)
                     /gene="hinT"
                     /locus_tag="KJL94_000285"
     CDS             complement(24868..25227)
                     /gene="hinT"
                     /locus_tag="KJL94_000285"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBD4408790.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            25566..27751
                     /gene="fhuE"
                     /locus_tag="KJL94_000286"
                     /pseudo
     CDS             25566..27751
                     /gene="fhuE"
                     /locus_tag="KJL94_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(27811..29244)
                     /gene="ptsG"
                     /locus_tag="KJL94_000287"
     CDS             complement(27811..29244)
                     /gene="ptsG"
                     /locus_tag="KJL94_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBD4408791.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(29539..30336)
                     /locus_tag="KJL94_000288"
     CDS             complement(29539..30336)
                     /locus_tag="KJL94_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4408792.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(30347..31351)
                     /gene="holB"
                     /locus_tag="KJL94_000289"
     CDS             complement(30347..31351)
                     /gene="holB"
                     /locus_tag="KJL94_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBD4408793.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(31348..31989)
                     /locus_tag="KJL94_000290"
     CDS             complement(31348..31989)
                     /locus_tag="KJL94_000290"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBD4408794.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(31979..33001)
                     /gene="yceG"
                     /locus_tag="KJL94_000291"
     CDS             complement(31979..33001)
                     /gene="yceG"
                     /locus_tag="KJL94_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBD4408795.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(33004..33813)
                     /gene="pabC"
                     /locus_tag="KJL94_000292"
     CDS             complement(33004..33813)
                     /gene="pabC"
                     /locus_tag="KJL94_000292"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBD4408796.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(33933..35174)
                     /gene="fabF"
                     /locus_tag="KJL94_000293"
     CDS             complement(33933..35174)
                     /gene="fabF"
                     /locus_tag="KJL94_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBD4408797.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(35261..35497)
                     /gene="acpP"
                     /locus_tag="KJL94_000294"
     CDS             complement(35261..35497)
                     /gene="acpP"
                     /locus_tag="KJL94_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD4408798.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(35708..36442)
                     /gene="fabG"
                     /locus_tag="KJL94_000295"
     CDS             complement(35708..36442)
                     /gene="fabG"
                     /locus_tag="KJL94_000295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBD4408799.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(36455..37384)
                     /gene="fabD"
                     /locus_tag="KJL94_000296"
     CDS             complement(36455..37384)
                     /gene="fabD"
                     /locus_tag="KJL94_000296"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBD4408800.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(37400..38353)
                     /gene="fabH"
                     /locus_tag="KJL94_000297"
     CDS             complement(37400..38353)
                     /gene="fabH"
                     /locus_tag="KJL94_000297"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBD4408801.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(38421..39491)
                     /gene="plsX"
                     /locus_tag="KJL94_000298"
     CDS             complement(38421..39491)
                     /gene="plsX"
                     /locus_tag="KJL94_000298"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBD4408802.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(39572..39745)
                     /gene="rpmF"
                     /locus_tag="KJL94_000299"
     CDS             complement(39572..39745)
                     /gene="rpmF"
                     /locus_tag="KJL94_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBD4408803.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(39797..40318)
                     /gene="yceD"
                     /locus_tag="KJL94_000300"
     CDS             complement(39797..40318)
                     /gene="yceD"
                     /locus_tag="KJL94_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBD4408804.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            40517..41101
                     /gene="yceF"
                     /locus_tag="KJL94_000301"
     CDS             40517..41101
                     /gene="yceF"
                     /locus_tag="KJL94_000301"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBD4408805.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(41213..42172)
                     /gene="rluC"
                     /locus_tag="KJL94_000302"
     CDS             complement(41213..42172)
                     /gene="rluC"
                     /locus_tag="KJL94_000302"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBD4408806.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            42745..45930
                     /gene="rne"
                     /locus_tag="KJL94_000303"
     CDS             42745..45930
                     /gene="rne"
                     /locus_tag="KJL94_000303"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBD4408807.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(46126..47079)
                     /gene="flgL"
                     /locus_tag="KJL94_000304"
     CDS             complement(46126..47079)
                     /gene="flgL"
                     /locus_tag="KJL94_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBD4408808.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(47091..48733)
                     /gene="flgK"
                     /locus_tag="KJL94_000305"
                     /pseudo
     CDS             complement(47091..48733)
                     /gene="flgK"
                     /locus_tag="KJL94_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(48799..49740)
                     /gene="flgJ"
                     /locus_tag="KJL94_000306"
     CDS             complement(48799..49740)
                     /gene="flgJ"
                     /locus_tag="KJL94_000306"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBD4408809.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(49740..50837)
                     /gene="flgI"
                     /locus_tag="KJL94_000307"
     CDS             complement(49740..50837)
                     /gene="flgI"
                     /locus_tag="KJL94_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBD4408810.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(50849..51547)
                     /gene="flgH"
                     /locus_tag="KJL94_000308"
     CDS             complement(50849..51547)
                     /gene="flgH"
                     /locus_tag="KJL94_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBD4408811.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(51600..52382)
                     /gene="flgG"
                     /locus_tag="KJL94_000309"
     CDS             complement(51600..52382)
                     /gene="flgG"
                     /locus_tag="KJL94_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBD4408812.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(52520..53271)
                     /gene="flgF"
                     /locus_tag="KJL94_000310"
                     /pseudo
     CDS             complement(52520..53271)
                     /gene="flgF"
                     /locus_tag="KJL94_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(53291..54499)
                     /gene="flgE"
                     /locus_tag="KJL94_000311"
     CDS             complement(53291..54499)
                     /gene="flgE"
                     /locus_tag="KJL94_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBD4408813.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(54524..55219)
                     /gene="flgD"
                     /locus_tag="KJL94_000312"
     CDS             complement(54524..55219)
                     /gene="flgD"
                     /locus_tag="KJL94_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBD4408814.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(55231..55634)
                     /gene="flgC"
                     /locus_tag="KJL94_000313"
                     /pseudo
     CDS             complement(55231..55634)
                     /gene="flgC"
                     /locus_tag="KJL94_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(55638..56054)
                     /gene="flgB"
                     /locus_tag="KJL94_000314"
     CDS             complement(55638..56054)
                     /gene="flgB"
                     /locus_tag="KJL94_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBD4408815.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            56209..56868
                     /gene="flgA"
                     /locus_tag="KJL94_000315"
     CDS             56209..56868
                     /gene="flgA"
                     /locus_tag="KJL94_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBD4408816.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            56944..57237
                     /gene="flgM"
                     /locus_tag="KJL94_000316"
     CDS             56944..57237
                     /gene="flgM"
                     /locus_tag="KJL94_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBD4408817.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            57242..57658
                     /gene="flgN"
                     /locus_tag="KJL94_000317"
     CDS             57242..57658
                     /gene="flgN"
                     /locus_tag="KJL94_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBD4408818.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(57698..59233)
                     /gene="murJ"
                     /locus_tag="KJL94_000318"
     CDS             complement(57698..59233)
                     /gene="murJ"
                     /locus_tag="KJL94_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBD4408819.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(59400..60266)
                     /locus_tag="KJL94_000319"
     CDS             complement(59400..60266)
                     /locus_tag="KJL94_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4408820.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(60268..60915)
                     /locus_tag="KJL94_000320"
                     /pseudo
     CDS             complement(60268..60915)
                     /locus_tag="KJL94_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(60926..61510)
                     /gene="rimJ"
                     /locus_tag="KJL94_000321"
     CDS             complement(60926..61510)
                     /gene="rimJ"
                     /locus_tag="KJL94_000321"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBD4408821.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            61746..62954
                     /gene="mdtH"
                     /locus_tag="KJL94_000322"
     CDS             61746..62954
                     /gene="mdtH"
                     /locus_tag="KJL94_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBD4408822.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            63018..63665
                     /gene="grxB"
                     /locus_tag="KJL94_000323"
     CDS             63018..63665
                     /gene="grxB"
                     /locus_tag="KJL94_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBD4408823.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            63799..64359
                     /locus_tag="KJL94_000324"
     CDS             63799..64359
                     /locus_tag="KJL94_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4408824.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            64465..65511
                     /gene="pyrC"
                     /locus_tag="KJL94_000325"
     CDS             64465..65511
                     /gene="pyrC"
                     /locus_tag="KJL94_000325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBD4408825.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            65585..65830
                     /gene="dinI"
                     /locus_tag="KJL94_000326"
     CDS             65585..65830
                     /gene="dinI"
                     /locus_tag="KJL94_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBD4408826.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            66120..66374
                     /gene="bssS"
                     /locus_tag="KJL94_000327"
     CDS             66120..66374
                     /gene="bssS"
                     /locus_tag="KJL94_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBD4408827.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            66489..67607
                     /gene="solA"
                     /locus_tag="KJL94_000328"
     CDS             66489..67607
                     /gene="solA"
                     /locus_tag="KJL94_000328"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBD4408828.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            67655..67768
                     /locus_tag="KJL94_000329"
     CDS             67655..67768
                     /locus_tag="KJL94_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBD4408829.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            68029..68595
                     /locus_tag="KJL94_000330"
     CDS             68029..68595
                     /locus_tag="KJL94_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBD4408830.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            68599..69174
                     /locus_tag="KJL94_000331"
     CDS             68599..69174
                     /locus_tag="KJL94_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBD4408831.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(69216..70268)
                     /locus_tag="KJL94_000332"
     CDS             complement(69216..70268)
                     /locus_tag="KJL94_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBD4408832.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            70493..71413
                     /gene="lpxL"
                     /locus_tag="KJL94_000333"
     CDS             70493..71413
                     /gene="lpxL"
                     /locus_tag="KJL94_000333"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBD4408833.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            71585..71782
                     /locus_tag="KJL94_000334"
     CDS             71585..71782
                     /locus_tag="KJL94_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBD4408834.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGADFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            71806..>72314
                     /locus_tag="KJL94_000335"
     CDS             71806..>72314
                     /locus_tag="KJL94_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4408835.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPGFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
BASE COUNT        17604 a        19202 c        17962 g        17546 t
ORIGIN      
        1 acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
       61 tctgaacata aaacactatc agtaagttgg agtcattacc cggttgcaga taccagcctg
      121 tggatgctta acatggaata ccaactcact cttaactggc ccgattttct tgaacgtcac
      181 tggcagaaac gcccggtggt gttaaaacgc ggctttaata attttattga cccgatctct
      241 ccagacgagt tggcgggtct ggcgatggaa agcgaagttg acagtcgact ggtcagtcac
      301 caggatggca aatggcaggt cagccacggc ccgttcgaaa gctacgatca tctcggtgaa
      361 accaactggt cattactggt acaggcagta aaccactggc atgagccgac cgccgcgctg
      421 atgcgaccgt tccgtgaact accggactgg cgtattgatg atctgatgat ttctttttct
      481 gtacctggcg gcggcgtcgg cccgcatctc gatcagtacg acgtgtttat cattcagggt
      541 accggacgtc gtcgctggcg agtgggcgaa aagctgcaaa tgaaacagca ctgcccacat
      601 ccggatctgt tacaggtcga tccgttcgaa gccatcatcg atgaagagct ggagcctggt
      661 gatattcttt atattccgcc aggattcccg catgaaggct acgcgctgga aaatgcgatg
      721 aactattccg tgggttttcg cgcgccaaat acgcgggaat taattagcgg atttgccgat
      781 tatgtgctgc aacgtgaact gggcggcaac tactacagcg atccggatgt tccacctcgt
      841 gctcatcctg cggatgttct gccgcaagag atggataaac tgcgtgagat gatgctcgaa
      901 ttgatcaacc agccggaaca ctttaagcaa tggtttggcg agtttatatc ccagtcacgt
      961 catgaactgg atatcgcgcc gccggaaccg ccttatcagc cagatgaaat ctacgatgcg
     1021 ctgaaacaag gtgatgtgct ggtgcgcctg ggtggtctgc gcgtattgcg cattggcgac
     1081 gacgtgtatg ccaatggtga gaagatcgat tccccgcacc gtccggcact ggatgcactc
     1141 gccagcaaca ttgcgctgac tgcggagaat tttggcgatg cgctggaaga tccgtcattc
     1201 ctcgcgatgc tcgcggcgct ggtcaatagc gggtattggt tcttcgaagg gtaagtttgg
     1261 atttaagccg gatgcagcat cacatgccgc atcccttttc gcttacttcc gttgcgccgt
     1321 taactcggca atacggacga tcacctgcac cgctttttcc ataccttcca gaatcacaaa
     1381 ctcatgctta ccatgatagt tgtaaccgcc agtgaacagg ttcgggcaag gtaatcccat
     1441 aaacgacaac tgcgcgccgt cggtaccgcc gcggatcggt ttcagttccg gttcaatatc
     1501 gcagtcacgc atcgcctgct gggcgatatc gagaatatgc ggatgctcaa ccactttctc
     1561 gcgcatattg tagtaactgt cttcaatcac caattcaatg tagcaatcag gatgtaaccc
     1621 tttgcccact tttttggcga tctccatcat tttacgttta cgcgcttcaa actgtttacg
     1681 gtcgaaatca cggatgatgt agtgcatatc ggcccgttca acggtgcctt tcatgctcgc
     1741 caggtgatag aaaccttcat agccttctgt catttccggg ctttcatccg ccggaacttc
     1801 cgcatgaata cgtgccgcca gcgacagcgc atttaccatc actcctttcg ccgtgcccgg
     1861 atgaacattg ttaccgacaa tttttatatt gaccgatgcg gcgttgaagt tttcaaactc
     1921 cagttcgcct acgccaccac cgtcaacagt gtaagcccag cgggcatcga aggcatcaac
     1981 atcaaaatgt ttcgcccctt tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc
     2041 accatgcgga atgtttttct gttgcaatac cgccagcgcg gtcatgattt ctgcaatacc
     2101 tgctttgtca tcagcaccta acaaggtttt accatcggtg gtaatcaacg tctgacccag
     2161 tagctgatgc agcaccggga acataaccgg tgataaaact tcatcgccga tacccagcgc
     2221 aatatcgcca ccgcgatagt tttcaacaat ttgcggatta acatttttgc cgctgcaatc
     2281 cggtgaggta tccacatgag aaataaagcc aatcgccggg atatcgccag ggacgttagc
     2341 cggtaacgtc gccatcaaag tgcccttctc acttaaggtc acattgataa gccccatttc
     2401 ttcgagctgc tctttcagca gatgcaataa cttccattgg ccttccgtgc tgggaacctg
     2461 tctcacccct gcttttgatt gggtatccag agacacgtag ttcaaaaatc gctcaagtag
     2521 tttatccatg tagtcaccct cactttttgt gacaacatta ttaagaagca agaaaagaca
     2581 aatattgcgt caggtcactt ttacccctgc aagcgggaat atttatcagc attaccttaa
     2641 tgaatataaa gctaagccag taattcacaa caaagattgg cgattcaagc ggtttgccgt
     2701 agaattaccg ccctcattaa gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa
     2761 gccatccgtt gcgtttacat gggacagagt aaaaaattga ataaacaacc gagttcgctt
     2821 tcaccgctgg tgcaattggc gggaattcgc aaatgctttg atggtaaaga ggtcattccc
     2881 cagctggatc tgactatcaa caatggcgag ttcctcacgc tgcttggccc ttctggctgc
     2941 ggtaaaacaa ccgttcttcg cctgattgca ggtctggaaa ctgttgattc cggacgcatc
     3001 atgctggata acgaggacat cacccacgtt ccggcggaaa accgctatgt gaacactgtt
     3061 ttccaaagct atgcactttt cccccacatg accgtgttcg aaaatgtggc ctttgggttg
     3121 cgcatgcaaa aaacccccgc tgctgaaatt acgccccgcg tgatggaagc cctgcggatg
     3181 gtgcagctgg aaaccttcgc tcaacgtaaa ccgcatcagc tctctggtgg tcaacagcaa
     3241 cgcgtcgcca ttgctcgcgc ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg
     3301 ctctcagcgc tggattacaa actgcgtaag caaatgcaga acgagctgaa agcgttacag
     3361 cgtaagcttg gcattacatt cgttttcgtg actcacgacc aggaagaagc actcaccatg
     3421 tcggacagga ttgtggtgat gcgcgatggt cgcattgagc aagacggcac gccgcgtgaa
     3481 atctacgaag agccgaaaaa cctgtttgtt gccgggttca ttggcgaaat taatatgttt
     3541 aacgccacgg tgatcgagcg actcgacgag cagcgcgtac gcgccaacgt tgaaggccgc
     3601 gaatgtaata tctacgttaa cttcgccgtt gaaccggggc aaaaactgca tgttctgctg
     3661 cgcccggaag acttacgtgt tgaagagatt aacgacgaca accacgctga agggctgatt
     3721 ggttacgttc gtgagcgtaa ctacaaaggc atgacgctgg agtcggttgt tgaactggaa
     3781 aatggcaaga tggtgatggt cagcgaattc ttcaatgaag acgatcctga ctttgaccac
     3841 tctctcgacc aaaaaatggc cattaattgg gtagaaagct gggaggtcgt actggctgat
     3901 gaagaacaca agtaagttcc agaatgtagt gattgtcact attgtcggtt ggcttgtgtt
     3961 gtttgtcttt ctgcccaacc tgatgatcat tggcaccagc tttttgaccc gcgacgacgc
     4021 cagtttcgtc aaaatggtct ttacgctgga taactacacg cgtctgctcg atccgctcta
     4081 ttttgaagtg ctattgcact cgctgaatat ggcgctgatc gccaccctcg cctgcctggt
     4141 gctgggctat ccatttgcct ggtttctggc gaagctgcca cacaaggtgc gtccgctgct
     4201 gctgtttctg ctgattgttc cgttctggac caactcattg attcgtatct acgggctgaa
     4261 aattttcctc agcaccaaag gctatctcaa cgagtttttg ctctggctgg gcgttatcga
     4321 cacaccaatc cgtatcatgt tcacgcccag tgcggtgatt atcggtctgg tttacattct
     4381 gctgccgttt atggtgatgc cgctgtactc cagtatcgaa aaactggata agccgttgct
     4441 tgaggcggcg cgcgatctcg gtgccagcaa gttacagact tttatccgca tcattattcc
     4501 actgacgatg ccggggatta ttgccggatg tctgctggtg atgctgccag cgatgggcct
     4561 gttctatgtg tccgacctga tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa
     4621 ggtacagttc ctcaatattc gtgactggcc gtttggtgca gccaccagca ttacgctgac
     4681 tatcgtaatg ggcctgatgt tgctggttta ctggcgcgct tctcgtttgc tgaataagaa
     4741 ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg
     4801 tacctgtata tcccaatcat tattttgatt gtgaactcct ttaacagctc gcgctttggc
     4861 atcaactggc agggttttac caccaaatgg tatagcctgc tgatgaacaa cgacagcctg
     4921 ttacaggcag cgcagcattc actaacaatg gcggtgtttt cggcgacgtt tgctacgctt
     4981 atcggttcac tgacggcagt tgcgctgtac cgttatcgct ttcgcggtaa gccgttcgtt
     5041 agcggaatgc tgtttgtggt aatgatgtca ccagatatcg tgatggcaat ttcgctgctg
     5101 gtgctgttta tgctgctggg tattcagctt ggcttctggt cgctgctgtt ctcgcatatc
     5161 accttctgcc tgccatttgt ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg
     5221 cggatgctgg aagcggcgaa agatctcggt gccagcgaat ttaccattct gcgaaaaatc
     5281 attctgccac tggcaatgcc agcggtggcg gcgggctggg tgttaagctt taccctgtcg
     5341 atggacgatg tggtagtttc ttcattcgtc accggaccaa gttatgaaat tctgccgtta
     5401 aaaatttatt cgatggtcaa agtcggcgta tcgccggaag ttaacgcgct ggcaaccata
     5461 ttactggtgc tgtcgctggt gatggttatt gccagccagc ttattgctcg tgataaaacg
     5521 aaaggtaaca caggggacgt taaatgaaaa aatggtcacg ccacctgctc gcggcgggtg
     5581 ctctggcact gggcatgagc gccgctcacg ccgatgacaa caacacgctg tatttctaca
     5641 actggaccga gtacgtgccg ccaggactgc ttgaacagtt caccaaagaa accggtatta
     5701 aggttatcta ttcgacttac gagtcgaacg aaaccatgta cgccaagctg aaaacgtaca
     5761 aagacggtgc ctatgacctg gtggttcctt caacctatta cgtcgataaa atgcgtaaag
     5821 aggggatgat ccagaagatc gacaagtcga agttaacgaa cttcagcaat ctcgatccag
     5881 acatgctcaa caagcctttt gacccgaata acgactactc cattccgtat atctggggtg
     5941 cgacggcgat tggtgttaac ggtgatgcgg tggatccgaa atctgtcacc agctgggccg
     6001 atctgtggaa gccagagtac aaaggcagcc tgctgttgac cgacgatgcc cgtgaagtgt
     6061 tccagatggc gctgcgtaag ctgggctact ccggtaacac caccgatccg aaagagattg
     6121 aagctgcata taacgagctg aaaaaactga tgccaaacgt cgcagcgttt aactccgata
     6181 acccggctaa cccgtacatg gaaggcgaag ttaacctcgg catgatctgg aacggttctg
     6241 ctttcgttgc acgccaggcg ggtacgccta ttgacgtggt gtggccgaaa gaaggcggca
     6301 tattctggat ggacagcctg gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga
     6361 aattgatcaa cttcctgctg cgcccggatg tggcaaaaca ggttgctgaa actatcggtt
     6421 atccaacgcc aaaccttgcg gcgcgtaagc tgttaagtcc agaagtggca aacgataaga
     6481 cgctctaccc ggatgctgaa accattaaaa atggcgaatg gcagaatgac gttggcgcag
     6541 ccagcagcat ttatgaagag tattatcaga agctgaaagc aggacgttaa ttcagcaagc
     6601 gggcggtggt attaccgccc gttttttagt tagcaaggat gtggctaatg agtcaggaca
     6661 gtaaagtttt atcccgctct ttcgtcggta ttgtggtccg cttgtaggcc tgataagcgt
     6721 agcgcatcag gcaatgcttc ctgctttata atccctccag caacttttca acaaattctg
     6781 gcaccacctg gcttgccggg ccgtaatatt tctcggcaaa ttcattacca acctgactcg
     6841 gttcaagatt cagttcaacg gtgtgagcac catgtagttt ggcttcgtgt acaaacccag
     6901 ccgccggata gacatgcccg gaagtaccaa tggcaataaa gacatccgcc atcgacaacg
     6961 ccatataaat ttcatccatg ccgagtggca tttcgccaaa ccaaacgacg tgcgggcgca
     7021 agggtgccgg gaactggcaa caatggcatt tatcttctgg cgtaacgtct ccggtccagt
     7081 cgagaacctg accactttgc gaacaacgaa ctttcagcaa ttccccatgc atatgaatga
     7141 cattggtatt acctgcgcgt tcatgcaggt tgtctatatt ctgcgttacc agcaaaaagc
     7201 gatcgccgag agcgtcttgc agtttagcca gcgcaagatg cgcggcgtta ggctgaattt
     7261 ctggctgctg cagctgtcga cgacgggcgt tataaaacgc ttgcaccagt tcaggatcgc
     7321 gatcgaaacc ttccggcgtt gccacatctt caacccgatg ttcttcccac aggccatctg
     7381 cggcgcgaaa ggtacgaata cctgattccg cagaaattcc tgcccctgtc agtacgagta
     7441 ctcttggttt ttccattgct tccggcacca ctttatctct gaaaaaaata cgctgacgca
     7501 aacgctcgcg caggcgacgt ttatttttac gaaaacgact taaccgatga ccccgacgcg
     7561 acagcatagc aacctctgtt gtttaatcgg ttagatgtag gaaggccgca ccacgcattc
     7621 ctcccgcatc accgtggcgc gcgcgttcaa tgcgcggaac acgagctaca ggtaagagat
     7681 gacaaggcaa cctgtccgcc agttgcgttg tgattgccgg gaaattcgat aagccaccac
     7741 caatgacgac caggtcaggg tcaacaatgg tcaggatatt tcccagagaa accgctaata
     7801 aatccagata acgctcaacg tgcgcccttg cctgctcatc gccttgatca taaagcgcaa
     7861 taatttcggg agcctgcaac ggttgatgat aatagtgttg atacagccac gcaaaaccgc
     7921 gaccagacag ataattttca atgcagccat gctgaccaca gccgcagcgg cgtaacggga
     7981 aatccaaccc catcatggtt aacgcatcaa ccggcagacg catatggcca aactcgccgg
     8041 taatgtagct tttaccagta atcggtttgc cgttgaaaac cagcccgccg ccaacgccgg
     8101 tgccgagaat taaccccatc accagtggat attgcgtaaa ttcatcatcc caggcttctg
     8161 aaagggcaaa acagttggca tcgttatcaa ggcgtacatc gcgatcaaga cgtgcgctca
     8221 ggtcggcacg cagcggtttg ccgctggcag cggggacatt agcggcatac agcttaccat
     8281 cttctgtttc cggcattccc ggaataccga tgccgacaga acctttacag ccaaaacgtt
     8341 ggtcagcttc agctaccagc tcacacactg catctaaaaa tgcgtcatag ctgtcacgcg
     8401 gtgtcggcac ccgcttttcc cactgcaact gccgaccgct atcaaacacg ccaagcgcaa
     8461 tttttgttcc accaatatca aacccgtaat acattgccgc tccttgtttt aatgtactgc
     8521 ctttactggc cgctaaggac tcgcgcaggg tcaatattac tggcgcgccg cgccggatac
     8581 caacttgcca aaagactcag caacaatgct gtgaccagta cgtagaagac gtccagccag
     8641 tgcaattccg atggcaggaa gtcaataaaa taaatatcgc tggagaggaa ctgatgaccg
     8701 atcagctttt caatccactc aataatcggg gtaagttgca gtgaaacaac tacgccgata
     8761 accaccccac acaggctgcc gaacagccct gccagcaatc cataccagac aaagatggcg
     8821 cgaattaaac catctttcgc ccccagcgtt cttaatactg cgatatcgcc actcttgtct
     8881 ttcaccgcca tcactaaggt ggagacgatg ttgaaacagg ccacgccaat caccagtacc
     8941 atcgccagat acataatggc gcggatcatc tggatatcgc gatacatata gccgtaagta
     9001 ccaatccaac ttttaatata aacatagctg ttggtcactt cacccgcatc gcgtaccagc
     9061 ttattggcgt tgaaaacatc cgtcatttta agggcaatac ctgacacgct ggaacccata
     9121 tcaagatatt gttgggcatc cgccagcggg atcatggcaa aactgtgatc gagttgacca
     9181 ctcaactgca aaataccggc aatgtgcaaa cgcacacgtt ttggctgcat cagtttatgc
     9241 tcgggattcg agttggggat catgatcgac acccaatcgc cctgcttcac tttcagcgca
     9301 tccgccacgc ctttgccgat gataatttgc tgttcgcccg ctttaaaatt gcgccacgca
     9361 tccccctgaa caaacgaggg taatgcgctc agacgctgtt cctgttgtgg gttaacgccc
     9421 ttcacctgga ttgcgcgcag attcgctcca ctttccacca gaccggtgaa attgatatac
     9481 ggcgcagcgg cggcaatacc cggcactttc tgcacgttat ccagtgcttc ctgccagtta
     9541 gtccatggct gatttaccgc ttcgatttcg ccatgcggca ccaccgccag aatgcggtta
     9601 ttcagttcgc gttcaaagcc gttcatcgcg cttaagccga cgatcaatac cgccacgccg
     9661 agggcaatgc caatggtaga aatgacagag attagcgaca ccatgccgcc gcgtcgccgt
     9721 ccgcgactaa aacgcaggcc aatcaataac gataaaggca tcgccattac tccgccccca
     9781 tcaggctcag ttccgccgtc agacgtccat cacgcatttc cagttggcgg ctcatacgtt
     9841 tcgccagttg caggtcgtga gtgaccacca ggaaagcggt gccctgcaag cgattcaact
     9901 ccccaagcaa ctggaagatg ctgtcggcgt tacgcgcatc gaggttaccg gtaggttcat
     9961 ccgccagtac cagacgcgga ttattgacca gcgcacgggc aatcgctaca cgctggcgtt
    10021 cgccgccaga aagttcagat gggcggtgat tcgcacgatg ctccagcccc accgctttta
    10081 acatctccag tgcacagctg ttgatttcag cgggcttttt cttgccaatc agcagtggca
    10141 tagccacgtt ttccagggca gtaaaatccg gcagcaggtg gtgaaactga taaataaagc
    10201 ccagcttctg gttgcgcagt tctgctttcg ccgctgaaga cagtttgctc atcggctgac
    10261 cgttaaagat cacatcgccg gaggttggcg tatccagccc gcctaacagg tgcagcaagg
    10321 tacttttacc ggaaccagag ctaccgacaa ttgccatcat ttcaccttcg cccacgctga
    10381 aactgacatt gagcagcaca tcggtttgca cactgccttc ctgatagcgt ttgcacaggt
    10441 tgtcgcattg caacaggatc ttattcataa cgtaaagcct cagcgggttg tgtggcggca
    10501 gcgcgccatg aagggtaaag cgtagacagc agcgcgatag ccatcgccac cagcgcaata
    10561 acaatgacct gtaaaggttc gatagccacc ggcagcgccg cgccatcaag caggacgccg
    10621 attatcggca tcagattatt taactggctg gcaagcagtg cgccaagcgc cgctccgagg
    10681 attgcaccga taatcccggc gctagcccct tgcaccataa agaccatcat gatttgtcgc
    10741 ggagttaagc cttgcgtttg caggatcgcg acttcgccct gcttctccat caccatcagc
    10801 cccagcgagg taataatgtt aaacgccgca acagcgacaa tcaggctcag cagtaagccc
    10861 atcatatttt tttccatgcg tacggcctgg aacagctcgc ctttacggtc acgccagtcc
    10921 tgccatttgc tgccttcagg cagtttttgc tgacttaaag agtcaactct cagcggctca
    10981 tccagccaca aacgccagcc ggtaatattg gctgccggat aacgcatcag acgcgatgca
    11041 tcctcaatat tcaccagcat ttcatagcca tcgacttcac tgttggcggc gaaagtgcca
    11101 atcacattga acaggcgctg acttggaata cgccccatcg gcgtgaactg gctggcagat
    11161 ggcaccatca cgcggatttg atcaccgcga ttgacgccca actgtgaggc aagttgttcg
    11221 ccgaggatga cattatattt ccccggctcg aggtcagttt gtttcacatt gaccagatac
    11281 ggcgttagtg gatctttttg cgccgggtcg ataccgagca tcaccccgac cgccacgctg
    11341 cgcgcgcttt gcagtaccac atcaccggta gtaataggtg cgacgcgatt aacgccgtcc
    11401 agtttgactg ccgtttccgg gagttgctgc gggttaagag agccatgctc agaagagaga
    11461 attgcctgtg gcatcaggcc aaggatgttg ttttgcagct cgcgctcaaa gccgttcatc
    11521 actgacaata ctgtgaccag cgccatcacc ccgagggtaa tgccgatggt agaaagccag
    11581 gagacgaaac gaccgaagcg atccactgca cgcccacgca tgtaacgcag gccaataaat
    11641 agagcgacag gttggtacat gaaatccgtc tggttgctgt agcaaagaca cgagtatata
    11701 agcgaaagca agatgggtaa acgtctgaac tgtaagtgat cgtcagggct aatctgaaaa
    11761 gcccccgtaa atcaatcggc taaattgcca tgttttatca tcaatcttgc cgcctgatct
    11821 taatctgaaa aatcatccaa tacagacaga tacccattac acctcgggtc ataaccagca
    11881 ggatcaaccg catgtaaatt aaaaaagagc gtgttgttct gatgaaacaa aaagagctat
    11941 ggattaacca gatcaaaggg ttatgtattt gtctggtggt gatttatcac tcggtcatta
    12001 ccttttatcc gcatatgacc acttttcagc atccgttatc ggaagtcctg agcaaatgct
    12061 ggatctattt caatctttac cttgccccct ttcgtatgcc ggtttttttc tttatatccg
    12121 gctatttgat tcgccgctat atcgacagcg taccgtgggg aaattgtctc gataaacgga
    12181 tctggaacat cttctgggtg ctggcgcttt ggggcgtggt gcagtggctg gcgttaagtg
    12241 cgctgaacca gtggctggca cctgagcgcg atttaagcaa tgcctccaat gccgcttatg
    12301 ccgattccac cggtgagttc ctgcacggga tgatcaccgc cagcaccagc ttgtggtatc
    12361 tgtatgcgtt aattgtctat ttcgtggtat gtaaaatttt tagccgtctg gcgctgccac
    12421 tattcgcctt gtttgtactg ctgagtgtgg cggttaattt cgttcccacg ccgtggtggg
    12481 gaatgaacag tgtgatacgc aatttgcctt attacagcct tggcgcatgg tttggcgcaa
    12541 caataatgac ctgtgttaaa gaggtgccgt tgcgccgcca tctgctgatg gcttctttgc
    12601 ttaccgttct ggcggtcggt gcctggttgt ttactatctc gctgctgttg tcgctggtat
    12661 cgattgtggt gatcatgaag ctgttttatc agtacgagca acgattcggt atgcgctcca
    12721 ccagcctgct gaatgtgatt ggctccaaca ccattgctat ctacaccacc catcgcattc
    12781 tggttgaaat attcagctta actctgcttg cgcaaatgaa cgcagcacgc tggtcgccgc
    12841 aagtcgaact gacactcctg ctggtttatc cctttgtcag tttgttcatc tgtactgttg
    12901 cgggcttgct ggtaagaaaa ctttcacagc gtgcattcag cgatctgttg ttctccccgc
    12961 cttcactgcc cgctgccgtc agttactctc gttaaaccat caccccagcc ggggcgattc
    13021 aatttgctaa accatgtcga ttacggataa tgaacagcat tgcttatcag agatatgccc
    13081 ccatatgttg aggcatatcc taacgagaat ctgacaaccg ttatgcctga acaatatcgt
    13141 tatacgctgc ccgtcaaagc gggtgagcag cgtctgctgg gcgagttaac cggcgcagcc
    13201 tgtgcaacgc tggtagcgga aattgccgaa cgtcacgccg gtccggtggt actcattgca
    13261 ccagatatgc aaaatgctct gcgtttgcat gatgaaatca gccagttcac cgatcagatg
    13321 gtgatgaatc tggcggactg ggaaactctt ccctacgaca gtttttcgcc tcatcaggac
    13381 attatctcct cgcgcctttc caccctttac cagctaccga cgatgcagcg tggcatactg
    13441 attgttccgg tgaatacgct tatgcagcgc gtttgcccac acagttttct ccacggtcat
    13501 gcgctggtga tgaaaaaagg tcagcgcctg tcacgagatg cattacgaac ccaactggac
    13561 agcgccggtt atcgccatgt tgaccaggtg atggagcacg gcgaatacgc cacgcgcggc
    13621 gcgttgctgg atctcttccc gatggggagt gagctgcctt atcgtcttga tttctttgat
    13681 gatgaaatcg acagcctgcg ggtgtttgac gtcgacagcc agcgcacgct ggaggaagta
    13741 gaagcgatca atctgctgcc cgcgcacgaa tttccgaccg ataaagcggc aattgaactg
    13801 ttccgcagcc agtggcgcga taccttcgaa gtgaagcgcg atccggaaca tatttaccag
    13861 caagtgagta aaggcacatt acctgctggg atcgagtact ggcagccatt gttcttcagc
    13921 gaaccactgc cgccgctgtt cagttatttc cctgccaata ccttgctcgt gaatactggc
    13981 gatctggaaa ccagtgccga acgtttccaa gctgacacgc tggcgcgttt tgagaatcgc
    14041 ggcgtcgatc cgatgcgccc gctgttgcca ccacaatcgc tctggctgcg ggtggacgag
    14101 ctcttctcag agctgaaaaa ctggccccga gtgcagctaa aaactgaaca tttaccgaca
    14161 aaagccgcga atgccaattt aggtttccag aaactgccag acctggccgt tcaggcgcaa
    14221 caaaaagcgc cgctggatgc gctgcgtaag ttcctcgaga ctttcgacgg tccggtggtg
    14281 ttctcggtag aaagtgaagg tcgccgtgaa gcgctgggtg aactgctcgc acgaattaaa
    14341 attgctccgc aacgcattat gcgtcttgat gaagccagcg accgtgggcg ttatctgatg
    14401 attggcgctg ccgaacatgg ttttgtcgat aaggcgcgta atctggcgct gatctgcgaa
    14461 agcgatctgc tcggtgaacg tgttgcccgc cgtcgtcagg attctcgccg caccatcaac
    14521 cccgatacac tgatccgtaa ccttgcggag ctgcatattg gtcagccggt ggtccatctg
    14581 gagcacggcg tcggtcgcta tgccggaatg accacgctgg aagctggcgg cattactggc
    14641 gagtacctga tgctcaccta tgccaacgac gccaaactgt atgttccggt gtcgtcactg
    14701 catctgatta gccgttacgc aggtggcgcg gaagaaaacg ccccgctgca taaacttggc
    14761 ggcgatgcgt ggtcacgcgc gcggcagaaa gcggcggaaa aagtgcgtga tgtggcggcg
    14821 gaattgctgg atatctacgc gcaacgcgcc gccaaagagg gcttcgcgtt taaacacgat
    14881 cgtgagcagt atcagttatt ctgcgacagc ttcccgtttg aaaccacgcc ggatcaggcg
    14941 caggccatta atgcggtact tagcgacatg tgtcagccgc tggcaatgga tcgtctggtg
    15001 tgcggcgatg ttggctttgg taaaacagaa gtggcgatgc gcgccgcttt cctggcagta
    15061 gataaccaca agcaggtagc ggtgttggtg cctaccaccc ttcttgcgca gcagcattac
    15121 gacaacttcc gcgaccgttt cgccaactgg ccggtacgta tcgaaatgat ctcccgtttc
    15181 cgcagcgcca aagagcagac gcaaatcctt gcggaagtgg cggaagggaa aatcgatatt
    15241 ctgatcggta cgcacaaact gctgcaaagt gacgtcaagt ttaaagattt aggcctgctg
    15301 attgtcgatg aagaacaccg cttcggggtg cgtcataaag agcgcattaa agcgatgcgc
    15361 gcgaacgtgg atattctgac gcttactgca acgccgatcc cacgtacgct gaatatggca
    15421 atgagcggaa tgcgtgacct gtcgattatc gccacgccgc ccgcccgtcg tctggcagtt
    15481 aaaacctttg tccgtgagta tgacagcctg gtggtccggg aggcgatcct gcgtgaaatt
    15541 ttgcgcggag gacaggttta ttatctctac aatgatgtgg aaaacattca gaaagccgcc
    15601 gaacggctgg cagaactggt gcctgaagca cggattgcca tcggtcacgg gcagatgcgc
    15661 gagcgcgaac tggaacgggt gatgaatgat ttccatcatc aacgtttcaa cgtgctggtt
    15721 tgtaccacca ttatcgaaac cgggatcgac atcccgacag ccaacaccat tatcattgaa
    15781 cgtgcggatc acttcggtct ggcgcagctg caccagttac gcggtcgcgt cggacgttca
    15841 catcatcagg catatgcatg gctgctgacg ccgcatccaa aagcgatgac taccgatgca
    15901 caaaaacgtc ttgaagcgat tgcctcgctg gaagatctcg gggcaggttt tgcgctggca
    15961 acgcacgatc tggagattcg cggcgcgggt gaactgcttg gcgaagaaca aagcggttca
    16021 atggaaacca tcggtttctc gctgtatatg gagttgctgg aaaacgccgt cgatgcactg
    16081 aaagccggac gcgagccgtc gctggaagat ctcaccagcc agcaaacaga agtcgagctg
    16141 cggatgccgt cgctattgcc agatgatttc atccctgacg tgaacacgcg tctgtcgttc
    16201 tacaaacgta ttgccagcgc caaaacggaa aacgaactgg aagagatcaa agtcgagctt
    16261 atcgatcgct tcggcctgct gccagatccg gcgcgtaccc tgctggatat tgcccgtctg
    16321 cgccagcaag cgcagaaact ggggatcagg aagctggaag gtaatgagaa aggcggggtg
    16381 atcgaatttg ccgagaagaa tcacgttaat ccggcctggt tgattggttt gctgcaaaaa
    16441 cagccgcagc attaccgcct tgatggtccg acgcgcctga aatttattca ggatttgagt
    16501 gagcggaaaa cgcgtatcga atgggtacgc cagtttatgc gtgaactgga agagaacgcg
    16561 atcgcttaat gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    16621 tgcactttcc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    16681 acaataacct tatatttatt atgtattttt ggtgatgatc aaaacgcgtt tttctcgctg
    16741 gctaacgttt tttacgttcg ccgctgccgt ggcgctggcg ctaccggcaa aagccaacac
    16801 ctggccgctg ccgccagcga gcagtcgtct ggttggcgaa aacaaatttc atgtggtgga
    16861 aaatgacggt ggttctctgg aagccatcgc caaaaaatat aacgtcggct ttctcgctct
    16921 gttacaagct aaccccggcg ttgatcctta cgtaccgcgc gcgggcagcg tgttaacgat
    16981 cccgttgcaa accctacttc cagacgcgcc gcgcgaaggc attgtgatca acattgcgga
    17041 gctgcgtctc tattactacc cgccgggtaa aaattcggta accgtgtatc caataggtat
    17101 tggtcagtta ggtggtgaca cgctgacacc gacaatggtg accaccgttt cagacaaacg
    17161 tgcaaaccca acctggacgc caacggcaaa catccgcgcc cgttataaag cacagggaat
    17221 tgagttgcct gcggtagtgc cggctggacc ggataaccca atgggccatc atgcgattcg
    17281 tctggcggcc tatggcggcg tttatttgct tcacggtacg aacgccgatt tcggcattgg
    17341 catgcgggta agttctggct gtattcgtct gcgggatgac gatatcaaaa cactctttag
    17401 ccaggtcacc ccaggcacca aagtgaatat catcaacact ccgataaaag tctctgccga
    17461 accaaacggt gcgcgtctgg ttgaagtaca tcagccgctg tcagagaaga ttgatgacga
    17521 tccgcagctg ctgccaatta cgctgaatag cgcaatgcaa tcatttaaag atgcagcaca
    17581 aactgacgct gaagtgatgc aacatgtgat ggatgtccgt tccgggatgc cggtggatgt
    17641 ccgccgtcat caagtgagcc cacaaactct gtaaatcctg gataaaaaaa atccccgcga
    17701 cacggcatgt cgcggggaaa aaatcagcag tagcaggcat taatgagggt taatgcttat
    17761 ttataaatta ctgctgttcc atggagggta ttcggaccgg ttacagaagt aatacggaaa
    17821 gattttgcgc ccatctcatc cgctttttgc gccagctgct cttccagcga tcccagattt
    17881 gtccccgcgt tagcactgat agtaccgact ttttgttggc cttctggcgt tgactgaact
    17941 tcgacagccg caaagttggc aaatgacatg gagcttaaaa tcgccgcagc gatgaggttt
    18001 ttacgttttt cataatagtg gccttatgca gatgaatgac gtcgcaagaa attacttaac
    18061 gatcgataac taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat
    18121 cattaattaa tttaaaaatc tttaaatttc agtcaaatag aaagcaatta tttttatggg
    18181 atctggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
    18241 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc
    18301 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
    18361 ttatgaagcg acttctcttg cggacctcgt cgaagcaacc ggagccaaag cgcccacgct
    18421 atacgcggaa tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga
    18481 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
    18541 gctggctgac tattttgcgg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
    18601 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tagccaatac
    18661 gttgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
    18721 agcgcgcggg gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg
    18781 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
    18841 gattgccgga acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaggc
    18901 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
    18961 ttcaaaatca gttgcccgtt actatctagc gggatctgcg tacccggatc gcggtccatg
    19021 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
    19081 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
    19141 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg
    19201 acatctttac cgcgaccacc accaaactga tgccctatca cgccaccagc aacagcgccg
    19261 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
    19321 ttgcgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
    19381 tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac
    19441 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct
    19501 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
    19561 aaaagcgcgg gaatgactgg aaatacttaa ggattcggag gagtctgaag gaatgcctga
    19621 tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
    19681 ggcgtttata ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat
    19741 aacgcggtta atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc
    19801 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
    19861 cattgagccg cgcgtcaggt tacccatcag gctaccaacg gtggagaagt tcgacagcga
    19921 taccagcgaa ccatgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
    19981 cagaatgttg ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac gcggcggaac
    20041 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc
    20101 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttag tttcaagacc
    20161 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccatacca tcagatcagc
    20221 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
    20281 ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg tgggccgcag tagagatacg
    20341 tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc
    20401 gttggtcagg cctttgtaac cgtagctgtg cagttgcttg atcgcgttgt gcaattcagc
    20461 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgttcactt tgccatttgc
    20521 gcccaggttg gcagagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
    20581 ctggtgcggg ttatcgagga aaatgcagtt ctctttgacg cctggcgtat tgaaatcgtt
    20641 agaggtgcta cccagcgcca tgaccagggt gtcataggcg attttacgtt ccggaaccaa
    20701 cagttcacct ttctcgtcgc gtagttctgc aatagtgatt gttttcgctt cacgatcaat
    20761 atcaatgacg gaacccaact ggaactggaa accatgattg cgcgcatggg ccagatagct
    20821 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gtggtttcca
    20881 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttac gtcccagctt
    20941 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatctt
    21001 tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt
    21061 tattaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga
    21121 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctt
    21181 aaaagatagg taactttacc gggggagatc aaaaaataga gtttattgtt tattatttga
    21241 gcaaattcgg aagggtaata tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat
    21301 aagatgcgca gcatcgcatc aggcattgat gctcgactgt cggatgcggc gtggacgcct
    21361 tatccgactt acagaattcg gagcgcatca ggtaccggca aggttacctg atgcgacgag
    21421 ggcatttacc cgagggtttt gaacgcttta atgcgctgta aatgcgggga gatattcttg
    21481 aatttgtgcg tctgctcttc gtcccagaca atctcgtaat aatgatgcaa ctcttcagat
    21541 gtccgctggc tgttaagtgc ttcatcatta cgcgacaaaa taaccagaca acgatcgcga
    21601 ttcttctcgc ggaagttggt cacacactta gtggcaatat cggcatactc ttccgggcga
    21661 tcaatcttgc cttccatgtt ctcataaggg aacaaattag ggttgaagat cacctggcgg
    21721 atatcgcaaa gaaaaccaat ccgttccgcc cagtatccgc ccaggccaac gccgcaaatt
    21781 agcggacgct cgtcaacgtt cagttgcaac attttgtcca cttctttaag cagatgctgc
    21841 atatcatgtt tcggatgccg cgtactgtag cttatcaagc gtacatccgg gtcaataaac
    21901 tgcaattgta agactttctc gtggttaccc ggactgttag agtcaaaacc gtgtaaatag
    21961 ataatcatcg cttcctcaca taagccaggg ttagtgacct gctttctctt cctgccagcg
    22021 ttcatgtaac tgattcagac gggtgctgat cgctttccag cgagccgagt ccatcagttc
    22081 ctgtcgcgaa aatgaacctt tatgatacaa acgtgtaaca cgttctgcct tgatcggtga
    22141 cagattatct aacacgctga cggccccttt acgattattg cagaccagga tcatatcgca
    22201 acccgcatcc agtgatgcct gcccgcgttc ggcataactg cccataatcg cggcaccttc
    22261 catcgataaa tcgtcagaga aaatcacgcc gtcaaaaccc agttcctgac gcaaaacggt
    22321 tttcagccag tagggagaac cgctcgccgg acgcggatca acatcactgt agatcacatg
    22381 cgcaggcata atggcgtcga gtttattttc gcggattaag gaactgaaga ccgacatatc
    22441 tttagcgcga atctccgctt gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc
    22501 cgttactgca ccgtgtcctg ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat
    22561 accatcaata aaccggctgg caattgccag ggctttttgt ggatcggcat gataagaacg
    22621 ctcgccaatc gccgcgctga tatgcccgac atccagcact ggcgcaaagc tgatatcaat
    22681 atccatagcg atcatttcgc tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc
    22741 ctcttccatt cccaacagcg cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc
    22801 gcgaaaacgc tgcacgcgtc caccttcctg atctaccgcc accaccagat gattgcgcga
    22861 tgctgcgcgg atctggcgca ccagttcacg taactgggca ggatcatgat agttacgcgt
    22921 aaagagaatc agccctccca ccagcggatg cgccagtatt tcacgctctt ccgcgtccag
    22981 ttcgtaacct tcgacatcca acattactgg acccacactg ctctccttat tgttttatta
    23041 atagctgccg ccaggtgtca tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc
    23101 agcggtactc aaaccaccct cctttgagca tcagcagcca gggaaaccat cgcctgacct
    23161 gacgccataa ttgcgccgga taaatcttcg cgcgagtggc atagtcattg accaattgcc
    23221 ggtgctgttc agtattttcc acccacaccg ccgccagttc cagcgcgata tcaccatctc
    23281 cagcatactc ccagtcgatg agttttaacc ctgatgcgct atgcactaaa tttccggcgt
    23341 ggacatccat atgcaatgga cttaagcgta aaggtcgtgg ttcccgcgct ttgcgcagac
    23401 gttttaacat tcgcagccaa cccacagtcc gccgcgccgg atcgctttgc tgccagtaca
    23461 gttccagtaa gggcaacagc gttattcgcc agccaaaacg tggttgttga tgtagataat
    23521 acagcaagcc tgccagttcg ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat
    23581 agtcgactac catccagtca cggagatata aatgcggctt cggtgcaatg cttgcgggta
    23641 gttgtgataa agcccgatac tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg
    23701 gctgacgcac aacaaaacgc tggccctgat gttcaatgag aaaactaccg ccactaagcc
    23761 cactattaaa cgtcgtgacg ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc
    23821 gcgtgatggg attattgctg cgaaacggca cctttacctg accagataat ttcgcccgtc
    23881 tgcaccagca tcagctgcat ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag
    23941 tacagcacgt aatgagcgcc gacattgcgg gcaatgccta tggctttact acgggtgcct
    24001 aaactgtcct gcggcgacaa acctaactgt tgcttagcca tcgacagttg ctgggcggaa
    24061 accagggtaa atttcccgtt attagccagc gcatttcgca gcgtttcggt cgcttctgcg
    24121 gcattcagcg aaccgttagt acggttgtta acgctatcaa ccagcaggac gctacccgca
    24181 gtcaccccgt cagccccaag catcttactg accatcggct gcattgcgcc attccagtca
    24241 taatggcgaa tatgcggcgc aggcggtgcc gttcgatctt cgtgctcaat tgggcctggc
    24301 tgctgcggga tcgtcggcac cgagggcact gtggggacag gctgttgtgg ctcggctggt
    24361 tgttccggcg ctggtttcac ttcttctacc ggtgcaggtt cacgttgccc cacacacccg
    24421 gcgagaaaca tcgccagcgc ggtaatcaag gcgtagcgac tcatttttgt catcaagatt
    24481 caccccttta caaatataga taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc
    24541 cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat
    24601 gcatctccag ccctctggcg tcataccagt aaaaacgata atgaacggta acgggttctt
    24661 gcctttcgtt ataaagcgtt gaggatgctg aaggttgaat atcagacgtc gaaaggacgg
    24721 gcttttctgc actgatgccc gcagccaata aagatgactc catcaccagc gattgctcat
    24781 cattcaccgg aatttccgga tgtgaacgac agcccaccag cagtaacaac accagagaca
    24841 ccagcccaaa gcatcctttt ctcatcgtta cagaccttta tgcgccagca ttggtcccag
    24901 cggacggcca cccaacaagt gcatatggat gtggtaaacc tcttgtccgc catggcggtt
    24961 ggtattcatg atcagacgat agccatcttc ggcaatacct tcttgctcag caatttttgc
    25021 cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca gctgacacgt cgttcacagt
    25081 cggaatgaga atattcggaa tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg
    25141 aaacgccgtt accagatcat cctggtagac gatatcggag gggatctcac gacgaataat
    25201 tttgctgaat atagtttctt ctgccacgac attttccttt ttcaaatagc catgcgttgc
    25261 gccatgctac gccgcgccac actgcgagta tagagtatgg tcgagttaaa cctgctcttt
    25321 caatcttacc tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt
    25381 attcccattt ctgcctgcga atgcgctgaa aagacattct ctgtttggtt tacacgacaa
    25441 atatgaatgc gtatatttct catttgcatt tgcatttgca gcaaaattat tcgcacacta
    25501 aatataaccg ctcatcaccc tcctccggca tgagcctgac gacaacataa accaagagat
    25561 ttcaaatgct ttcaacacaa tttaacaggg ataatcaata tcaagtcatc accaaaccgt
    25621 cactacttgc cggttgcata gcactggcac tattaccttc tgccgctttt gctgcaccag
    25681 ccactgaaga aacggtgatt gttgagggtt cagccacagc tccagatgat ggcgaaaatg
    25741 attacagcgt aacgtctacc tctgcgggta ccaaaatgca gatgactcaa cgtgatattc
    25801 ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta caaacgctgg
    25861 gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc gatcgtgctc
    25921 tttattattc ccgcggattc cagatcgata actatatggt tgatggtatc cccacctatt
    25981 ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactgttt gaacgcgtag
    26041 aagtagtgcg tggcgcgaca ggactcatga ccgggacggg taatccatct gcggcaatta
    26101 atatggttcg aaaacacgcg acctgtcgtg aatttaaagg cgatgtctcg gcggaatacg
    26161 gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc gaagacggta
    26221 aaatccgcgc gcgaattgtc ggcggctacc agaataacga ctcatggctg gaccgctaca
    26281 atagtgaaaa gaccttcttc tcgggcattg tcgatgctga tttaggcgat cttacgacgc
    26341 tttcagccgg ttacgaatat cagcgcattg atgttaatag cccaacctgg ggcggtttac
    26401 cgcgctggaa tactgatggc agcagcaaca gttacgatcg cgcacgcagt accgcacctg
    26461 actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag cagcggtttg
    26521 ctgatacctg gcaagcgaca ctgaatgcca cccactctga agtcgaattt gacagcaaaa
    26581 tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg ccatacagta
    26641 attatggacc tggctttgat catgtcggcg gcaccggttg gaacagtggc aaacgtaaag
    26701 ttgatgcgct ggatttgttc gctgacggta gttatgaatt gtttggtcgt cagcacaatc
    26761 taatgtttgg tggcagttac agcaaacaaa acaatcgttc agttcatggg ccaacatctt
    26821 cccggatgaa attggcagtt tctacaactt taatggcaat ttcccacaag ccgactggtc
    26881 atcacagagc ctggcgcagg acgataccac acatatgaaa tcgttatatg ctgccactcg
    26941 tgtcaccctt gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt
    27001 tgatacgctg acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt
    27061 tgacatcaat gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa
    27121 tgatcgtgac agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg
    27181 tctgaaatcg gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga
    27241 gcaggataat gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc
    27301 ctataaagcg gtggatggga cagtcagtaa aggggtggaa tttgaactca acggcgcaat
    27361 taccgacaac tggcagctga catttggcgc aacgcgctat cttgcagagg ataacgaagg
    27421 aaacgccgtt aatcctaatc tgccacgcac cacggttaaa atgttcacca gctatcggtt
    27481 gcctgtcatg ccagagttga cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac
    27541 cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga
    27601 tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta caggggaacg tcaataacct
    27661 gttcgacaaa acctacgata ccaacatgga aggttctatc gtctacggcg caccgcgtaa
    27721 tttcagcatt accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg
    27781 gctgccttag tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc
    27841 ggttttcagg ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac
    27901 tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac
    27961 gctgacgcgc agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa
    28021 tgcagcaacc agagccggtg ccatttcgct ggtacctgtt gcttttgcat cttcagtcgc
    28081 gtcttcacga cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta
    28141 gtaaacaatc gcataaccga taccgacgat cggtaacagc cacagtttgc tgctgttacc
    28201 agacagaacg atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag
    28261 aagaatacag attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg
    28321 cgcaacgaac atgaagaaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag
    28381 cgccgcggag atcataatac cgcccacttt cgcacggttt tctggtttag cagagtgcca
    28441 gatagcaatt gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt
    28501 acccgcagtc gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc
    28561 tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc
    28621 aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg
    28681 gtaagcagcc cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga
    28741 cagcacaacg ccagtaaaga tggcagccag gccagaaatg atcggcacaa agcgtttacc
    28801 ggcaaagaag ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc
    28861 tgcaatcgca ccggagataa tccctccgag tacgccagta tccgccaagt gtttagaggc
    28921 gatttcttca gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat
    28981 gatgccatag gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag
    29041 ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc
    29101 cataacatgc gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag
    29161 aatacctgcg ataggtagta cggataccgg cagcatcagc gatttaccga ccttttgcag
    29221 gttagcaaat gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta
    29281 cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt
    29341 tatttattac acagagtaaa ataattcagt gccaatttgt ttgacggcta tcacgtttca
    29401 acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac
    29461 gcggttattc accgcccaat tttcggcggt gaactttact cgttttagcc attaattacg
    29521 agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag
    29581 acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac
    29641 agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg
    29701 taccggcgca aggtaaggtg agtcagtttc caccagtaat cgatccaggg ggacataacg
    29761 cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta
    29821 aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac
    29881 gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cacgggcgtc
    29941 acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa
    30001 ggactcttgc tgacgtactt tagtttccgg tgtgtaataa taatccagcc cggtttcacc
    30061 cagcgctaca acaccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc
    30121 attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag
    30181 atcccgcata tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc
    30241 gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag
    30301 accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa
    30361 ccggtagcac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga
    30421 gaagctcgcg gttgatgcct gtgacagaca ttaactgttc acgaatgtgg caaacatccc
    30481 ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc cagttcggcg accagtcccg
    30541 gcacatcaac attggtcacc tgcgcagcgc catgatggcg ttttagcgca tccatcagca
    30601 acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gccagcagcg
    30661 aataccagtc gcccgatggc acgctatatg ccaacgcctg acacaatgtt tcacgagcct
    30721 gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca
    30781 atgcggcaag taatgcatcc tgtgacattg tcacttcgcg tgaaagccaa gtcacggcgt
    30841 actgttctgg aggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta
    30901 aacgttcagg ctcgcgggta gccaggaaaa accaggtttc tgctggtggc tcttcaagcg
    30961 ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa
    31021 ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat
    31081 caatgcccag cgtatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc
    31141 cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt
    31201 ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg
    31261 cctgaatgag tagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga
    31321 aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttcaccc agtgggtcac
    31381 ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa tggtatgaat
    31441 acttttatct tgtgcagcca gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa
    31501 agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc
    31561 cggggtaaca tcgagataga gcgttaagtc tgggcgaaaa tccccgagaa cagcatcacg
    31621 cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg ccctgatacg cctgagtgga
    31681 aagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agcgcaggtt tgatgaccgt
    31741 ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac
    31801 ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt
    31861 accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcga gcgtctcaac
    31921 caccacatta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt
    31981 actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg
    32041 caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg
    32101 tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct atcgcacccg
    32161 gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag
    32221 aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc
    32281 gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa
    32341 cggcggtttc tttttcgata attgatgcca tcgtcaccag ctgattttta tctttataag
    32401 gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgaa
    32461 ctcgcttgag taacgcgaca tcggtggtat tggcggtata catcaaggtg tctggccaga
    32521 accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact
    32581 tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg aggtagtcgc
    32641 tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca
    32701 atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt
    32761 gagaaagatc cggttcgata cgcagcagcc attgaaaaac ccgtgggcga ttgatgatct
    32821 tatcagcata aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata
    32881 tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca
    32941 caccagcggc gatacccagt accaccagca ataacaagat aatcaataac acttttttca
    33001 tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa
    33061 ggtgacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat
    33121 ctcatctgcc tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga
    33181 ttgtgccagt aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag
    33241 tcgcggcgta taaactacat tgcccttccg ccagaacaaa ttagccgcac agcattccgt
    33301 aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga
    33361 gcgaatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg
    33421 ccccagccgc accgggctta gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg
    33481 tgcaggataa gccgtaacgg agagaatccg cgttgctggt ccgctgttca atgtgctgta
    33541 ccctcgcccg ccactaccgc gactgatcac gactttcagc acaccatttt gctgttctgc
    33601 tgccagcgtt ttcatctctt gttcaagctg aggccagaag tcacaggaaa tcatcaaccg
    33661 ctgacaagca tcctgtagtc gcaggatatg cgccgataac aaactgactt taccgtcgat
    33721 aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag
    33781 cgattcctgc ttataaccgt taattaagaa cataccggct ccttatcaga gaattgttag
    33841 tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa
    33901 gcggaccttt tatatgggtg gaaaatgaca acttagatct ttttaaagat caaagaacca
    33961 ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt
    34021 gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt
    34081 ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc
    34141 gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct
    34201 tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta
    34261 ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt
    34321 gccattgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg
    34381 ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt
    34441 tcgtactctt caagtaccag cataccggca ccatcgccca gtacgaagcc atcacgctct
    34501 ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc
    34561 gcgccaaaac caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc
    34621 acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa
    34681 gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga
    34741 cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca
    34801 ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca
    34861 attgcggcac caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg
    34921 gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg
    34981 cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag
    35041 gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc
    35101 caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca
    35161 cgacgcttag acacgtttgt cctccaagga gggaaaaatg attctagtgg gacaaaaaga
    35221 taaaactcag gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg tggccgttga
    35281 tgtaatcaat ggcagcctga acggtggtga ttttctcagc ttcttcgtcc ggaatctcag
    35341 tatcaaactc ttcttccaga gccattacca gctcaacggt gtcaagagaa tccgcgccca
    35401 ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg cttaacgccc agctgttcgc
    35461 cgataatttt cttaacgcgt tcttcgatag tgctcatact cttaaatttc ctatcaaaac
    35521 tcgctttcgc gatggttttc gtagtgtata aaatgttgaa aaatttgcaa ctaaatcccg
    35581 gcaggtctta ccacgatttt acgttatttt gaggccttac cccccaataa cgcaaatcat
    35641 tttgcactaa ttattactca taaccacgca gactgcgcgc aacatgagca ttttgtgcaa
    35701 atcgcggtca gaccatgtac atcccgccgt tcacatgcaa agtttcaccc gtgatgtaag
    35761 ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg ccgccgaggc
    35821 gacccgcagg aacctgcgcc aggatacccg cacgctggtc atcgctcagc gcacgtgtca
    35881 tgtccgtttc aataaagccc ggagcaacaa cgtttacagt aataccgcgt gacgcaactt
    35941 cgcgcgccag tgatttactg aagccgatca agcccgcttt cgccgcagcg tagttggcct
    36001 gaccgccatt tcccatggta ccaaccacag aaccgatagt gataatacga ccatgacgct
    36061 ttttcatcat agcgcgcatt accgcttttg acagacggaa aacagatgaa aggttagttt
    36121 cgataatatc gttccactct tcatctttca ttcgcattaa caggttatca cgagtgatac
    36181 cggcattatt gaccaggata tccacttcac caaattctgc gcgaattttt tccagaacag
    36241 attcgataga tgccgggtcg gtcacattca acatcagacc tttgccgttg gcacctaaat
    36301 aatcactgat cgcctgagcg ccattttcac tggtcgcagt gccaataact ttcgcgccac
    36361 gggctgcgag cgtttcagca attgcgcggc caattccgcg gcttgcaccg gttaccagtg
    36421 cgatttttcc ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc gctgccatcg
    36481 ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc gtcaggccag
    36541 taagcacctt gcccgggccg acttcataga gatgttctac gccttgcgct gccatgtact
    36601 caacagactt cgtccactga accgggttat acaactgacg taccagtgcg tcacggatgg
    36661 catcaccatt ggtttcgcat ttcacatcaa cgttattcac aacagaaact gttggtgcgt
    36721 taaaggtgat tttcgctaat tctactgcca gtttgtcggc tgctggtttc atcagcgcac
    36781 agtgagacgg tacgctcact ggtaacggca gcgcgcgttt tgcgccagca gctttacagg
    36841 cagcgccagc acgctcaacc gcttctttat gaccggcaat aaccacctgt cccggagagt
    36901 taaagtttac cggagaaacg acctgacctt ctgcagcttc ttcacacgct ttcgcaatag
    36961 gcgcatcatc cagaccgatg attgccgcca tagcgcccgt gccttccggt acggcttctt
    37021 gcatgaactt accgcgcatc tcaaccagac gcaccgcatc agcgaaatca atcacgccag
    37081 cgcaaaccag cgcggagtat tcccccaggc tgtgaccggc catcattgcc ggtgctttac
    37141 cgccctgctg ctgccatacg cgatacagcg caacagatgc agtcaacagc gctggctggg
    37201 tttgccaggt tttattcagt tcttcagctg gcccctgctg ggtcagcgcc cacaggtcgt
    37261 agcccagcgc cgcagaagct tcagcaaacg tttcttcgac aattggatag ctcgccgcca
    37321 tatcagccag cattccaacg gtttgagaac cctgtccagg gaacacaaat gcaaattgcg
    37381 tcatgtttta atccttatcc tagaaacgaa ccagcgcaga gccccaagtg aatccaccgc
    37441 caaaggcttc aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt acagcttcat
    37501 ccagcgcgca cgggacagag gccgcagagg tattaccgtg gcgatccagc gtcaccacga
    37561 cattgtccat cgacataccg agttttttcg ccgttgcact gataatacgc aggttagcct
    37621 gatgcggaac cagccagtcc agttgagaac ggtcaagatt attcgccgcc agcgtctcat
    37681 caacgatgtg cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc gccatcgtca
    37741 gatgaattga attctctgga ttcacgcggt cggcgtttgg cagcgtcagc aattcaccat
    37801 aactaccgtc ggcatgcaga tgggtggaaa tgattcccgg ctcttcagag gcagccagca
    37861 ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc acgatctgtt ggatcgcagg
    37921 tgcgcgccag tacatcggaa ccgacgacca gagcatactt caccgcccca gatttcacgt
    37981 attgatcggc tacgcttaat gcataggtga aacctgcaca ggctgctgca acgtcaaatg
    38041 ccgggcaacc tttaatgtcc aacatgcttt gaatctgaca agctgcgctc gggaaagcgt
    38101 gcgtagcaga agtcgttgcc acaacgatca ggccaatctg gtctttctca atgcccgcca
    38161 tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg tttggcgcgg
    38221 caatgtggcg ttcgcggata ccggtacgag tgacaatcca ctcgtcagag gtgtccacca
    38281 ttttttccaa atcggcgttt gtccgcactt gttcgggcag atagctgcca gtaccaataa
    38341 tcttcgtata catgtacgct cagtcacttt tcggttatat accgtcactt gcaaactgcg
    38401 agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc cagcagctca
    38461 aaaccagctg ggtatacaga ttccaggcga gcggcaattc gctgaggaac ttgtcgctgc
    38521 accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc tgcaccatga
    38581 ctttttatca ccgtgccgcg caatcctaac agacaggcgc cgttatactg gtcggggttg
    38641 aggtgactga atcgcctcgt caggctcttt tgtagccaac gctttaataa cagtagccac
    38701 cacgaccgtt ttttcccttc accctgagat ttcagcagag aaaggaacat cctgacaaca
    38761 ccttccatcg tctttaacgt gacatttcct gtaaagccgt cacaaaccag cacatctgtc
    38821 ttgccagtta acaactcatt ggcttcaaga tagccgatat aattgataga agggattgtt
    38881 ttaagcaccg ctgaggcatc ccgaatactg tcaagaccct ttacttcttc ttcaccaata
    38941 ttgagcaacg ccacgcgagg attgggaatt tccaccacct cttcagccag aactgagccc
    39001 ataatggcaa attgcaccaa cattgtgctg tcacaatcga cgttggcccc taaatcgagg
    39061 accaccgttt tgcccttttg ctgatgtggt aataccgtca ccagcgccgg acgctcaatc
    39121 ccctccaggg gcttgagtaa taattttgcc agccccatca gtgccccggt attaccggca
    39181 ctgacacagg cttgcgctcg accttctttc accagctcca gggccatacg cattgaactc
    39241 ccacgactgg cgcggatagc ttgcgaaggc cgggcatcac tggcgataac tgactgcgca
    39301 ggaataatct gcagacgcga acgttgttca aagtcagctt tagcaagtaa tggcgtgatg
    39361 gcgtcgggat tgccgactaa aagaagagtg agttgcgaat tagaattcag tgcctgcaat
    39421 gctgcaggca ctgtcacgga agggccaaaa tcccctccca tgacatctaa cgccagggtt
    39481 agacgtgtca aggtatcgtc gccgcccggt ttggtctttc cccagttgcc tggggaaaat
    39541 cctcactaag cttcatcacg cagacgcgtg attacttagc gatgaccttg cggccgcggt
    39601 agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc accagaagtt ttgtctacag
    39661 acaggctggt gactgcggtc agcgcgtcat gggaacgacg catgccacgt ttggaacggg
    39721 ttggtttatt ctgttgtacg gccatggacc ttactcctca attacggttt atccccgatc
    39781 caacggggag caccaattac ttacgcttta agctggctaa tacggcaaat gggtttggct
    39841 tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact tcacagtgtt
    39901 cagaatcatg caccggaact accggcaagg cgaggatgat ttcatcttca accattgcaa
    39961 gcagatcgat ttcaccgaat tcgttaacct caatcggttc atacgcttcc ggcagtgctt
    40021 cagcctgttc gtctgaacgc acaggactaa aacaatacgt tgtgtagacc tgatgagtaa
    40081 acggcttccc gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg ccgtttaaca
    40141 ctgcgagacg ttggttatcg atagcgaacg acatggagca ttccacatca ctgtccacac
    40201 tgactacgga ttcggcgacg cgctcaacct gatcaggggt atagataccc tggtaatcaa
    40261 ggcgtttttg agccgtacga accggatcga gagtcagggg taattttacc ttttgcatag
    40321 ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc tgtggttgcc
    40381 ttttgccaat aattcgcaca cattgcgggt tactgcttta ttttcgttca gtgctgataa
    40441 cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg gctatcatca atacgctata
    40501 tctgatggaa aaaaatatgc ctaaacttat tttagcctcc acctcgccat ggcgtcgcgc
    40561 tcttctggaa aaactgcaaa tctcttttga atgtgcagca cctgaggtcg acgaaacccc
    40621 acgcagcgac gaatcaccgc gacagttggt gcttcgactg gcacaagaaa aagcgcaatc
    40681 tctggcgtca cgttatccgg atcatttaat tattggttca gaccaggtat gtgttcttga
    40741 tggcgaaatc accggtaaac cgttaacgga agaaaatgcc cgtctgcaat tacgcaaagc
    40801 cagcggcaat atcgtcacct tctatactgg actggcgttg tttaattcgg cgaatgggca
    40861 tctacaaaca gaagtggagc cttttgacgt ccatttccgt cacctaagcg aggcggagat
    40921 tgataattac gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta agagtgaagg
    40981 atttggcatt acgctgtttg agcgtttaga gggacgtgac cctaacacgc tggttggttt
    41041 accgctaatc gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc tgatgggata
    41101 gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc ggtgcagacg gcctgatgcg
    41161 gcgtaaacgc cttatcaggc caaccgccca ttatcaggct tatatgcttg tattagcgcg
    41221 cgttacgcag cttttgcaaa caacgcttca aaccttcatc catcggcgct tcgatacgca
    41281 tcacctcacc ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg aacagacgat
    41341 ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg tagcgatcgt
    41401 caaaggcaat cggatgaccc gcatactgtg tatgcacacg gatctgatga gtacgtccgg
    41461 ttaccgggct acaacgcacc agggtggcaa atgcatagcg ttcttccact ttaaagcgtg
    41521 tttccgacgg tttgccttcc tgactgacac gcacgatacg ttcgccgctt tgcagaatat
    41581 ttttcaataa cggcgcttga acgctcttca catgcgactg ccactgaccg cgcaccagcg
    41641 ccaggtaatc tttttgcatt cctttttcac gtaattgctc atgcagagaa cgcaacgccg
    41701 agcgtttttt cgctaccagc aacacacctg aagtgtcccg gtcgagacga tgaaccagtt
    41761 caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc gataacgccg aagcttaaac
    41821 cactgccgcc atgtaccgcc gtaccggaag gtttattcag caccaggata tgatcatctt
    41881 catataagat gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa accgcctctt
    41941 cttcccgctc agcaacgcga accggtggga tacgcacctc atcacccgct tcgagtttat
    42001 attcaggctt aatacgtttt ttgttcaccc gcacttcacc tttacgcaaa atacggtaaa
    42061 tcatactttt tggtacgcct ttcaattggg tacgcaaaaa gttatcgata cgttgccccg
    42121 cttcgtcagc ggtgatagca acaattttta cggatggagt ctctgttttc atggtgggcg
    42181 attctaatta gccaacagga ttcgcgccac tcatttttct atgcttatat ttagttttgc
    42241 accttattac ttcactacgt gatcacttta ttgatggtta ttaaaccaat caccagcaag
    42301 aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag
    42361 gcttgcagtg gaataatgag gccgtttccg tgtccatcct tgttaaaaca agaaatttta
    42421 cggaataacc cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat
    42481 ctttcaggca gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg
    42541 gagagtaata cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg
    42601 caaccgacac tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag
    42661 acacgaggcc atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa
    42721 tgtaagaata atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa
    42781 gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca
    42841 gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt
    42901 ctggaagctg cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa
    42961 attgcccgcg aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat
    43021 gtgttgcgtg aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa
    43081 ggcgcggcat taaccacctt tatcagtctg gcgggtagct atctggttct gatgccaaat
    43141 aacccgcgcg caggtggcat ttctcgccgt atcgaaggcg atgaccgtac cgaattaaaa
    43201 gaagcactgg caagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc
    43261 gtaggcaaat ctgctgaggc gctgcaatgg gatttaagct tccgcctgaa acactgggaa
    43321 gccatcaaaa aagccgctga aagccgcccg gctccgttcc tgattcatca ggagagcaac
    43381 gtaatcgttc gcgccttccg cgattattta cgtcaggata tcggcgaaat ccttatcgat
    43441 aacccgaaag tcctcgaact ggcacgtcag catatcgctg cattaggtcg cccggatttc
    43501 agcagcaaaa tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag
    43561 tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc catccggtgg ttccattgtt
    43621 atcgacagca ccgaagcgtt aacagccatc gacatcaact ccgcacgcgc gacccgcggc
    43681 ggcgatatcg aagaaactgc gtttaacacc aacctcgaag ccgccgatga gattgctcgt
    43741 cagctgcgcc tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcca
    43801 gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg
    43861 cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg ccagcgcctg
    43921 agcccatcac tgggtgaatc cagccatcac gtctgcccgc gctgctccgg taccggtacc
    43981 gtgcgtgaca acgaatcgct gtcgctctct attctgcgtt tgatcgaaga agaagcgctg
    44041 aaagagaaca cccaggaagt tcacgccatt gttcctgtgc caatcgcttc ttatctgctg
    44101 aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc aggacggtgt tcgctgcgtg
    44161 attgtgccaa acgatcagat ggaaaccccg cactaccacg tgttgcgcgt gcgcaaaggg
    44221 gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg
    44281 ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac aacctgcact ggcaaccttt
    44341 gccatgccgg atgtaccgcc agcgccaacc ccagctgaac ctgccgcgac tgtcgtagcc
    44401 ccagcaccga aagctgcaac ggcaacacca gcagctcctg cacaacctgg gctgttaagc
    44461 cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg aagaagccaa accgaccgag
    44521 caaccaacac cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caaacctcgt
    44581 cagagcaacc gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc
    44641 agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca aactgccgag
    44701 acgcgtgaga gccgtcagca ggctgaggta acggaaaaag cacgtaccac cgacgagcag
    44761 caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa
    44821 gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt
    44881 gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag
    44941 caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa
    45001 ccaattgttc aggaagctcc agctccacgc acagaactgg tgaaagtccc gctgccagtc
    45061 gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt
    45121 ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt
    45181 cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc
    45241 acgtctccgg aattggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa
    45301 gatgtacaag ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact
    45361 gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg cgccagttgt tgaagaaatg
    45421 gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc aaccggaagt ggttgaaacg
    45481 acgcatcctg aagtgattgc cgccgcggta actgaacagc cgcaggtgat taccgagtct
    45541 gatgttgccg tagcccagga agtcgcagaa cacgcagaac cggtggttga accgcaggaa
    45601 gagacggcag atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa
    45661 gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggtg
    45721 gccgcggttg aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc
    45781 gctccggcac cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact
    45841 tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc
    45901 gctcctgcgc gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta
    45961 actgccaggg cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat
    46021 ccttgctaac caacaatgca aaataggcaa aaaaatgccc gccccggcga tcccagagcg
    46081 ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt tgttgagctg
    46141 gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca atgccgtttg
    46201 ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg
    46261 ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca gtttgttcag
    46321 ctgcgtgcct aattccgcgc gaacggtaag gacattattc agcgagtttt tcagtccacg
    46381 gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat ccgcgaccgg
    46441 cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct
    46501 accgtctggt tccgctaccg cgttactggt aatactgtcg aaaattttgt cgcccgtatg
    46561 tcctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg ttccgccaac
    46621 ataatcaccg ttagcctcac taaacggcgc agtctctgtt ttataaccgg caaaaatgta
    46681 gcgcccatta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctgaat
    46741 atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat
    46801 tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct cttccagtga
    46861 cactttttga gtggcgaaag tacgtgccag cgtgtactgg ctgttttgcg cctgtgcctg
    46921 ggagagaaca acagcttgtg atgcagcaat gggatcgtct gaagggttaa cgactcgctt
    46981 acccgtcgac atctgctcgc cgtacttcat ccattctgcc tgagaattgg tgatgccgcg
    47041 catgttttgc tggtacatca tctgggtact gaaacgcatt ttatctcccc ttagcgaatg
    47101 ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa
    47161 tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac
    47221 tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact ggttttcaac
    47281 gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc
    47341 gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc gttgcggttg
    47401 tcgctatcac ccgcatcttc ttcgctcgcc atcgcgattt tcgcttcgtc ggtgattaat
    47461 acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc gttaacggca
    47521 ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatctggt
    47581 gtcgccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt attatcgaac
    47641 gagattttgt aatctgtcgc cagtaccacg gaggcatcag ttaccgtggc accgatcgca
    47701 acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagt aaagaaatct
    47761 tcaccagcat cgccgttagc atcaaatccg gctttgtgtt ggctgttgaa agcctcggca
    47821 aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat
    47881 gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct
    47941 gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga aggaactgcc
    48001 gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggcatc gtgatgttat
    48061 aagtaccgcc atcctgaacg ctgacttcta cgccaacaat ctggtttaat tcgctcacca
    48121 gttgatcgcg ttgatccagc agattgttag gtgacgcccc tgcccccacg cctgtcaggc
    48181 gcgagatttg atcgttcagg ctggcaattt gtttagcgta attgttgatc tgatcaacgc
    48241 tggcacctat cgcgatattg acctgtttgt cctggtcgcg cagatattga tcggtggttt
    48301 taaactgatt caccaaccct tctgatttcc caatcagcgc ctggcgcgcc gccgggtctt
    48361 ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg
    48421 aagaggtact ggtggagagc atattgtcga ttttcgacat ctgctcatag cgggcggtca
    48481 ggccgctact ttgcgtctgc gcagcacgta actggttggt aataaacgta tcatactcac
    48541 gctgcacacc agaaacgtag acgccattgc caacccagcc gccagcgccc aacgtgctat
    48601 tggcctgcgc cataatagtg gtttggcggg tatatccggc aacgttatag ctggagatat
    48661 tattacttgc cgtatttaac gccgcctggg ccgcgttcag tccgctcatg gcgttattga
    48721 tcaagctgga catggaggtt ccttataagc cttcaattac agagtattat cggcagcgac
    48781 ccgccggact tgagttattc agaacagatt atcaatgttc atgctgtagg ttttgctcac
    48841 cttgtcgctt atcgatttca tctgctggat catgttggtg agtttacggg catagtgagg
    48901 atcggtagca tagcccgcgt cctgtaggac ctgcgccccc tgttccgcac tcgcggcggt
    48961 cgtcacggcg gcgtagcgcg ggttacgtgt taacagccca acgtaatccg acaatgcttc
    49021 cagatacgag ctgtagaccc gaaacttcgc ttttactttc ttcgcttcgc cgttttcata
    49081 ttcagtcgtg gtgatttcag tgacttgccc tttccagttg ccagaggctt tgacaccaaa
    49141 caggttatag cttggctcgc cgttttcgcg gcggatttgc cgttgccccc aaccagattc
    49201 aagcgcagcc tgagcgagga tcaaatgatg tggcacaccg ctttgctggc ttgccagttg
    49261 ggcgggcagc gagagttgcg cgaggaatgc tctactgtca cccggcagcg aatcatcgta
    49321 gttacgtggt acggcctttt gcaccagctg cgaaagcgtc tggttttgat aacgcaccac
    49381 ggtttcgagc gggaatttca tcggtgctgc tggcgtggac tcctctggca atggttgttc
    49441 tggcgtcatc tgtttaacca tcatctctgc aagccccaga cctttgcccg ccgtcatctg
    49501 ttgggcaatt tgctggtcat acatactggt atacagacga gtgtgctcgc tgctgaacag
    49561 gccatctttt ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga acatcccttc
    49621 cacctgacgg gccaccggac ggatatttgc cgccggatct tcgctcgctt tcgcctttag
    49681 ttcgttgagt gattgcgcat cccaggccgc acttgccagt agtttgctgt cgctgatcat
    49741 cagatgattt ccagttttgc ccgcagacat cccgcacttt gcattgattg caaaatagac
    49801 atcagatcca tcggcgtagc gcccagcgca ttgagcgcac gcaccacgtt attgaggctg
    49861 gcgctggaac gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat ctgcgtttgt
    49921 ggagtaacaa cagtctgtcc accaccaaac ggtgtatctg gctggctgac attggcctga
    49981 cgattaactg ttactgagag atttccctgc gctaccgcgc agctgtcgag ggtcacttcg
    50041 cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta ctttagcgtc ctgcggggtg
    50101 acattaacct gcatattctg gatatcggca aggaaacgga cctgggaact gttgccactt
    50161 ggtacgcgca cctgaatagt ccgcgcatct aacgcggtgg cgctgccata tccacgcacg
    50221 cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat cttcgtcgtt aagttgcaaa
    50281 ttaagggtat tcccgacgcc aaactggctg ggcaattcac gttcaataac cgcaccattg
    50341 gtgatccgtc caccgttcag ttggttaacc tgaacactgc taccgcctgc ggaggctcct
    50401 gcgccgccaa ccagaatatt gccctgcgcc agcgcataca cctgactgtc aacgccctta
    50461 agcggtgtca tcaacaacgt acctccacgc aagcttttgg cattacccat ggaagaaacc
    50521 accacatcga tggtttgccc ctgacgtcca aacggaggaa gtgacgctgt caccattacc
    50581 gcagcgacgt tttttagctg catattggtg cccgtcggaa cggtaattcc cagctgtgag
    50641 agcatgttat taagcgtttg tgtggtaaac ggcgtctggg ttgtctggtc accagtgcca
    50701 tccagcccca ctaccaagcc atagccaatc agtgagtttt gccttacccc ctgaacactg
    50761 gtgagatcgc gaatacgctc agcctgagcc gccgtcgtaa ccagtagaag aattaatgca
    50821 gagagaaatt taatcaccac agcctcactt acattggcga caggttaagg aagaaacgct
    50881 gcaaccagcc catattttgc gcttcgttaa tgtagccatt gcctacgtat tcaatacgcg
    50941 catccgccac ctgagtagac ggtacggtat tgctgccgct gatagtgcgt ggattaacca
    51001 cgcccgagaa gcgaataaat tcggtaccct gattaatggc aatctgtttt tcacccacca
    51061 catgcaggtt gccgttgacc agtacctggt caaccgtcac cgtcaacgtg ccgctaaagg
    51121 tattgctggc attggccccg ccctttccgt tgaacgtgtt accaccggag gcttcgacat
    51181 cggcacgagc gttaccaaac agcccctgca aatagcgcgg cacagtatca aagccaaaat
    51241 tagttttacc gtcacggctg gcattcgcag aggagctttt gctggcgctg acgttctcct
    51301 gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg acgatcttca aacagcggtt
    51361 gatagccata gttaatcggc tgagcagact ggaaaataga accgttggcg acgggcgtcg
    51421 gaccgggaac cggttgtgca ctggtcgccc cctgcaccag cggcgtggag ggtatccagg
    51481 cgcagccggt tagtgaaagc accaacaagc tggaaatggc ataagtatgc gcagcgtttt
    51541 tttgcatggc tatcatcttc aaaaatcagt aaaccggtga acctgccacc ggttatgcct
    51601 tagagttgcg tcagtttttg cagcatctga tcggtggtgg acaccgcttt actgttgatt
    51661 tcgtaagcgc gttgcacctg aatcatattg accagttctt ccgccacgtt gacgttagac
    51721 gtttcaacat acccttgata cagcagtccc gcgccgttca ggcccggcgt gctttcgttc
    51781 ggtgcaccgg aggattgcgt ttcggtgtag aggttttcgc caatgctctc cagtccggta
    51841 tcattcataa aggtggtgag attgagctgc ccaacctgaa ccggagctgc ctggccttgt
    51901 tgggttacgc tgaccacgcc atcacgaccg atggtgatac ttaacgcatt cgccggaatg
    51961 gtgatcgctg gctgcacctg aaaaccacca gccgtcacca gctgcccgtt ctgatccacc
    52021 tggaaagagc cgtcacggat ataggctgat gagccatctg gcaacattac ctggaaaaag
    52081 ccctgccctt taatcgcgac atctttgctg ttattggtct gcgacagatt gccctggctg
    52141 tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga tttgtaatcc ggagggtaag
    52201 gtggtttgtt cggaagactg tgcccccggc tggcgaatgg tttgataaag cagatcttca
    52261 aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt ttgccaggtt gttggcaatg
    52321 acgtccatat tggtttgctg ggcgtcaagg cccgttttgg cgatccataa tgaactgatc
    52381 atataatgtc ctgtagtgga ttgctgacaa gcagagcaga agcacgcgac agtgtgaatt
    52441 tcccctcacc ctaaccctct ccccaaaggg gcgagggaat agtccgcgcg cttttattgc
    52501 aatgtcatca atcgttcaat taactcatcg acagcagttg gttggcacgg cctgcgttat
    52561 catcgacgct gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt
    52621 cgctcattgc cgcaacggca ttgacgttac tgccttccag aacccccgac atcacacgca
    52681 aggttggatc tgcctgcagt accggccctc gcgtggcctg ggtttctgcg cttaaacgaa
    52741 aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagt ttcagacgcc
    52801 ctactggcgc aactgtattt gccggatcgc ccggattgag cgccgagatt gtgccatcgg
    52861 cagcaatcgt gatttccgcc ccttccggca cagcaattgg ccccgcctcg cctatcaccg
    52921 gatgcccctg aattgtcagt tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat
    52981 acccttcgct gccgtcagcg gtctgcacgg ccagccatcc tgctgcaacg caacgtccag
    53041 cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct gcacccggtg ttgacgccgt
    53101 taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa
    53161 ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta ctggcggtta ccgcctgttg
    53221 attcagcgtc tggctggctg ctcccatcgc agtataaatt gcgtgatcca ttgagctatc
    53281 ccgtcagcga ttagcgtaag ttaacccgcg tgttgaggat ctggtcctgg gttttgatgg
    53341 tctgggcgtt agacttatag ttacgctggg caacgatcat attgaccagt tctttactga
    53401 gatcgacgtt ggacgcttcc agcgcaccgt tggtcagggt gccaaagttt cccgtcccgg
    53461 ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg
    53521 atgccagacc ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc agttgggttt
    53581 gttcgttgga attgttgccg acaaccgtac cgtcatcatt gatttgataa ctcaccagat
    53641 cgcccggttt gtagccattc tgggtggttg ccacaatgtt gttagcgccg gtattttgct
    53701 gcatggagtt gaggaagctc agactaaacg tggcgggttc tgcaccgtta attgcgccgg
    53761 ttgcgatatt attcgccatc gcaccatcca ctaatgtgcc attagcatta aattccagtg
    53821 ttgtcgctgt cttcgcaatg ctgtttggat cactgctatc ctgggtgtag acctgccagt
    53881 tattatcccc ggtcttcaca aaatagacgc tcatgtcatg agcattaccc tgactgtcga
    53941 aaacagtcac cgaacctttt ttgttatagc tatccgcatt gctggcgctg aatggcgtaa
    54001 cagtaggaag cggatcactg gaattcaggt tgatctgcat cgacgccgtg gtggtagttt
    54061 tcgctgccat cagggtattc gggatcgaaa tattggtcgg attcgcccct tgctgaatag
    54121 tcggcggcgt accggttacc gggtaacccg tcagctgtaa accttgcgta ttcaacaggt
    54181 tacggttttc atccagctta aattgtccgt tacggctgta gaacaccgaa ccgttgctgt
    54241 ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc cagacctcgc ccggtgttgg
    54301 tggtcgtgcc atcggtaaag tcctgagtga taccggcaac ttttaccccc agtcccactt
    54361 tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga tttaaagccg taggtggcgg
    54421 agttggcgat attgttgcca ataacatcga ggttggtggc ggcagcgttt aatccgctaa
    54481 ccgcttgaga aaaggccatg actgactcct gaaatgtgaa ggcttaaatt atctgccgta
    54541 cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag cgtattaccg ctgttgccgc
    54601 ggatcacacc ctgcaccaga gcaaactgca gcggctgggc aaccagttgt gtaccaccgt
    54661 tactggcgct aatcgctaca ttgtaagaac cgttcggcgc agtgctgcca tcagtcaacg
    54721 aaccgtccca ggtgaaactg tgaactccgg cggtcagttc accaatatca atggtgcgca
    54781 caaccgcgcc atttttatcg gtgatggtgg ccgtaacttt gtctgccgcc tgttgcagct
    54841 caacaccaaa cggcgtggtc gtggtcacag ccccttcttc actgccggtt cccgcaagaa
    54901 cagttgtgcc ggggatcatc acgccgtgac cgatcaggtt actggcctgt aacgactggc
    54961 tgttatcaat ctgtccggaa atagatccga gcgtggtatt gagtttttca atcccgctga
    55021 ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt
    55081 cctggttttt cagctgcgcc accagcaaag tcagaaaact gctttgtaaa tctgcggcgt
    55141 tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc ggtatttgtc ggatcggtgg
    55201 tggttaccgc aatggacata gctttctcct ttattgaccg agcgtaaggg ttttcagcat
    55261 catgcttttc accgtgttga gcacttcaac attggcctga tagctgcgtg acgccgacat
    55321 ggtgttaacc atctccccga cgacatcaac gttcggcatt tttacgtagc cctttgcatc
    55381 tgccagcgga ttacctggtt cataaaccag tttgtccggg gcctgacttt cttaacatcg
    55441 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    55501 tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg
    55561 gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    55621 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    55681 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    55741 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    55801 agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga ggtcatcgtc
    55861 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    55921 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    55981 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    56041 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    56101 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatggt
    56161 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctgacaata
    56221 aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtacggc gagcaatctc
    56281 acatcgcaat tgcacaactt ttttagcgcc caactcgcgg gggtaagtga tgaggttcgt
    56341 gtttctattc gtacagcgcc caatctacta ccgccatgcg agcagccatt gctttcgatg
    56401 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    56461 cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcctatt
    56521 gtgcggggag gaaagctgga agctggcaat gtcaaactga aacgtggacg cctggatacc
    56581 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag cctgcgcgat
    56641 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    56701 ggacaacgcg tcaatgtgat cgccagcggt gatgggttta gcgccaacgc agaaggtcag
    56761 gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc gggacaggta
    56821 gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat
    56881 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    56941 accatgagta ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca accgcgcgaa
    57001 accaccgacg cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc caccagcacc
    57061 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    57121 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    57181 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    57241 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    57301 ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca gccagttgca
    57361 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    57421 caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    57481 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    57541 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    57601 tggggcgaac ggtcagacct caacacccca tcgcggcagt aaaaagattt cgatctgaat
    57661 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    57721 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    57781 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    57841 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    57901 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    57961 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    58021 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    58081 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    58141 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    58201 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa agaatgccca
    58261 acgcaaccgc actcggcagt gccaacagga aacaaagacg caacccccag tccatcaaac
    58321 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    58381 tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg gcgtaataca
    58441 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    58501 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    58561 cagcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    58621 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    58681 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    58741 ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    58801 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    58861 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    58921 cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca gaaacataag
    58981 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    59041 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    59101 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    59161 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    59221 ttaataaatt catcggtgtt ctaatccgga cccacgggca aaacgcctgc aaggcaggcg
    59281 ctgaaagagg cgaatagtct acctagatta tggtgaattg ctaccgccag atgttacagg
    59341 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc
    59401 tattcgccgg cggtttgcgg aactgtctga ttttgcacgc attcaatgaa gtgccgcgca
    59461 cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg tttattcact
    59521 acaccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg
    59581 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaagtggtg
    59641 atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    59701 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    59761 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacgctattg
    59821 ctacgatgtt tatccattct tagcgaggct gcggtggcga gttgcgtttt taactcaccg
    59881 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc
    59941 agttccacca gcctttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    60001 tgtacccccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggagtgc
    60061 acaaaaaccg catcacaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    60121 cagctgtcac aaattggcag ggctttggcg cgcgtaggcg accaggctcc ttgtaacgtc
    60181 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc
    60241 actacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    60301 acgttgtttc agttctgcca cttcgatttc cagggcttcg acgcgggtct ataaatcacc
    60361 gtcaaccatg ttcgacatag ccgttaccgc cggctgatct tcaacctcac cactgaaaag
    60421 atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    60481 atcttcgcga ttcgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    60541 atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac gcaataacaa
    60601 cgtggtgatt aacgccactt ctgcggcgct cagtttcaga tcgccaaatt ctgaattaca
    60661 aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc gtagataatg
    60721 acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat tcatgaccgg
    60781 tttacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    60841 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatt acgcgagctt ccaaggcagt
    60901 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    60961 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    61021 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    61081 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    61141 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    61201 catgaaaaga gccgcgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    61261 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    61321 ctagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    61381 gcttgaggaa atggcgattc tctgcgtaat aatccgccag acgccaggca tcacgatcat
    61441 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    61501 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    61561 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    61621 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    61681 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    61741 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    61801 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    61861 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    61921 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    61981 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    62041 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    62101 cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgtggtcgt tttttctcgc
    62161 tgttgatgat gcaggacagt gccagtgcgg tcattggcgc attgttgggg agctggctgt
    62221 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctatgtgcgg
    62281 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    62341 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    62401 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    62461 ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    62521 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    62581 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggcctacaac
    62641 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    62701 aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    62761 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    62821 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct
    62881 tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt cgtttgctgg
    62941 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    63001 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    63061 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    63121 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    63181 acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa ctcgacggca
    63241 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    63301 gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    63361 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg
    63421 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    63481 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    63541 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    63601 ctaattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    63661 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    63721 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    63781 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    63841 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    63901 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    63961 catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa taaggttacc
    64021 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    64081 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    64141 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    64201 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    64261 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    64321 gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    64381 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc
    64441 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    64501 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    64561 accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccaca
    64621 gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc cgggcacgat
    64681 ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    64741 ggatttaatg aagccgtgtt caccgctgca aaactttacc cggcaaacgc aaccactaac
    64801 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    64861 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    64921 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    64981 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    65041 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    65101 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    65161 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    65221 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    65281 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    65341 gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa cgacacattc
    65401 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    65461 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    65521 cttcctgttg tcattaggtt attttacctg tataaataac cagtatgttc aacagggggc
    65581 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    65641 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    65701 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga
    65761 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    65821 cagcgaataa tatgcagtga tttttgtttt tgccggatcg ccccggcttt tttattttac
    65881 ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca atatgctaaa
    65941 aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag aaatttatct
    66001 gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca
    66061 gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga
    66121 tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg
    66181 ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc
    66241 aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc agacaattta
    66301 tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt
    66361 atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac
    66421 catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag
    66481 agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg
    66541 ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc
    66601 atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg
    66661 aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca
    66721 acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg
    66781 catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg
    66841 atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct
    66901 tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac
    66961 tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg
    67021 acgatgatgg cgtaactatt gaaacggttg acggtgagta tcaggcgaaa aaagcgattg
    67081 tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag cccgtacgta
    67141 aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt
    67201 ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt
    67261 tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt
    67321 ttgcggaagt ggccagtgat gggtcagaag ccttcccgtt cttgcgcaat gtattgccgg
    67381 gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt
    67441 ttattatcga taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc
    67501 acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg caagaccaaa
    67561 aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc
    67621 ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc actatctcat
    67681 caataatatt cgcgagcatt tgatgcttta tctttttctc tggggattgt tggcaattat
    67741 ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg acaaatattt
    67801 aatctccctt tcatttagcg tgaaaaattg aatgatatta ttcctgcttc tacagcactc
    67861 accagacatc tctgatgctg aacccactaa acttatgaag attctttcat tattaattca
    67921 aaattattga aataaaaata aatatcattc aatttgttat ttttgtgttg caattcattc
    67981 tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca
    68041 aatacccctg aacgctatgg tgttatatct gcggccttcc attggctaag cgcaattatt
    68101 gtctacggca tgtttgcttt aggtctgtgg atggtcacgc tcagttatta cgatggctgg
    68161 tatcacaaag cacccgaact gcataaaagc atcggtattt tgttaatgat ggggctggtt
    68221 attcgcgttc tgtggcgtgt catatctcca ccgccgggtc cgctgccgag ttattcgccc
    68281 atgactcgtc ttgccgcaaa agctggacat ctcgcattgt acctattgct gttcgccatc
    68341 ggcatcagcg gctatctgat ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg
    68401 tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg cagactttgc cggtgccctg
    68461 catttttggc tcgcgtggag cgtcgtagta ctgtctgtta tgcacggatt tatggccctg
    68521 aagcatcatt tcatcgataa agacgacact ctgaagcgca tgctgggaaa gtcgtcatct
    68581 gactatggag tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg
    68641 ttctctgccg gttcagcggt tgccgccgat tacaaaattg acaaagaagg tcagcacgcc
    68701 tttgttaact tccgcataca gcatcttggc tatagctggt tatatggcac ctttaaagat
    68761 ttcgacggta cttttacctt tgatgaaaaa aatccggctg ccgataaagt gaatgtgaca
    68821 attaacacca ccagcgtcga tactaaccac gccgaacgcg ataaacatct tcgcagtgcg
    68881 gattttctca ataccacaaa atatccacag gcaacattca cctccaccag cgtgaagaaa
    68941 gacggtgacg aactggatat taccggcgat ctgacgctga atggcgtaac caaacccgtc
    69001 acgctggaag cgaaattaat tggtcagggc gacgacccat ggggcggtaa acgtgcaggc
    69061 tttgaggccg aaggcaaaat taagctcaaa gacttcaata tcaagacaga tttaggtcca
    69121 gcttctcagg aagtggatct gattatttca gtggaaggcg tacagcagaa gtaagagtga
    69181 aatgttgccg gatggcacgc atccggcaat gatatttatt ctgttggatc aggaataccc
    69241 agtgttgtat tcagacgtcc acgagactta ttaaagatct tattgccatt ttcacgtcct
    69301 gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca aatctcacta
    69361 caacaacctt tgtatttttc cgcgcatact ggacactgaa taaacagcag gtggcagcca
    69421 tcatttttac agttggtatg gctgtcgcac ggcgcgccgc actggtggca atgcgcgata
    69481 atctcatccg atatacgttc gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa
    69541 cgcaccggca agccctgctc gcgcgcctta cgggcgtatt caataattcc gccctcgata
    69601 tgccagactt tgttgaatcc gttatgtttc atccaggcac tggccttttc acaacgaatg
    69661 ccgccggtgc agtacatgac gattttttta tctttatgtg cctgcatcat ctcgactgct
    69721 tttggcagct gctcacggaa ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc
    69781 acttcatact catagtggtt acgcatgtcg ataaatagtg catcgggatc gtcaagcatg
    69841 gcgttcactt ccgccgcttg cagatactca ccaacattgc tggcatcaaa gtgaggatcg
    69901 tcaataccgt cggcaacaat gcgatcgcgt accttcatgc gcagtaccca gaaggatttc
    69961 ccgtcatcat ccaacgcgat attcaggcgt aaaccctcta aagccgggtc gaaggcatag
    70021 agctgcgcgc gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc gttaatgccc
    70081 tcatgcgcca gatacactcg cccaaaaaca ttcagcgcgg taaacagctg atataaagcg
    70141 tcacgggtcg ccttaggatc ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc
    70201 ggttcgctct cagccaacat tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac
    70261 actggcatgg tgtacggttc ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc
    70321 aattatcgat aattaacatc cacacatttt acgctacatt tgcgcattaa aaattatttg
    70381 ttatttacaa gcgcggcaat ttcgcccagt cttcagccac aaattttggt tgcgggcgaa
    70441 aaaatgcgac aatacataca attgcccgta taggttgaaa aacaggattg atatgacgaa
    70501 tctacccaag ttctccaccg cactgcttca tccgcgttat tggttaacct ggttgggtat
    70561 tggcgtactt tggttggtcg tgcaattgcc ctacccgatt atctaccgcc tcggttgtgg
    70621 attaggaaaa ctggcgttac gttttatgaa acgacgcgca aaaattgtgc atcgcaacct
    70681 ggaactgtgc ttcccggaaa tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt
    70741 cgaatccgtt ggcatgggcc tgatggaaac cggtatggcg tggttctggc cggaccgccg
    70801 aatcgcccgc tggacggaag tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa
    70861 acgcggcatc ctgttagttg gcatccattt tctgacactg gagctgggcg cgcggcagtt
    70921 tggtatgcag gaaccgggga ttggcgttta ccgcccgaac gataatccac tgattgactg
    70981 gctacaaacc tggggccgtt tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa
    71041 aggcatgatt aaagccctga aaaaaggcga agtggtctgg tacgcaccag atcatgatta
    71101 cggcccgcgc tcaagcgttt tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac
    71161 cggaacctgg atgctggcac ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg
    71221 taagccagat ggcaaagggt atcaattgat tatgctgcca ccagagtgtt ctccgccact
    71281 ggatgatgcc gaaactaccg ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat
    71341 ggcaccagag cagtatatgt ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc
    71401 ttcacgctat taaatctccc atgccggatg cttcagaatg gcatccggca ttaccacagc
    71461 aaatccccct gatttagcga taaaagctct ctggattgcg ccccctggaa gtcgggcgca
    71521 taattagtgt gtttatcttt tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat
    71581 tgctatgtca ccctgtgaaa atgacacccc tataaactgg aaacgaaacc tgatcgtcgc
    71641 ctggctaggc tgttttctta ccggggccga cttcagtctg gtaatgccct tcttacccct
    71701 ctacgttgag cagcttggtg ttaccggcca ctccgccctg aatatgtggt ccggtattgt
    71761 cttcagtata atgggttcct aaacacatca ttttaggtat tgaatatgag acgggcaatc
    71821 agctacatca gattttcaag tgaacgacaa ctgaaagggg actctgtacg ccgacaatct
    71881 aagctggtta ctgactggct tgataagaac cctgggtttt acttggattc atccttgagc
    71941 tttaaagatt tggggaaaag tgcttttagt ggtaagcacc tgaaaggtgg tctaggtgac
    72001 tttttaaccg ctatcgagaa agggcttgtt aaagctggtg atacgctact aatcgagagt
    72061 ttagaccgtc tgtcacgtca ggatattgat attgcttctg agctattacg ccgtatctta
    72121 agggctggtg tagatgtagt cacactgagc gatggagaac attacacacg agaaagttta
    72181 aaagacccgt tagccctgat taaaagcatt ctgataatgc agcgggcgca tgaggaaagt
    72241 ttgagaaagt ctgagcgtgt ccaagctgca tggaaccgta aaaaagaact catctccgaa
    72301 ggtatcaagg tatc
//
LOCUS       DAEBXD010000005        71408 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEBXD010000005 DAEBXD010000000
VERSION     DAEBXD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71408
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.005"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(661..2256)
                     /locus_tag="KJL94_000336"
     CDS             complement(661..2256)
                     /locus_tag="KJL94_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBD4408836.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2611..4782)
                     /gene="glcB"
                     /locus_tag="KJL94_000337"
     CDS             complement(2611..4782)
                     /gene="glcB"
                     /locus_tag="KJL94_000337"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBD4408837.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4804..5208)
                     /locus_tag="KJL94_000338"
     CDS             complement(4804..5208)
                     /locus_tag="KJL94_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBD4408838.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5213..6432)
                     /gene="glcF"
                     /locus_tag="KJL94_000339"
                     /pseudo
     CDS             complement(5213..6432)
                     /gene="glcF"
                     /locus_tag="KJL94_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6443..7495)
                     /gene="glcE"
                     /locus_tag="KJL94_000340"
     CDS             complement(6443..7495)
                     /gene="glcE"
                     /locus_tag="KJL94_000340"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBD4408839.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7495..8994)
                     /gene="glcD"
                     /locus_tag="KJL94_000341"
                     /pseudo
     CDS             complement(7495..8994)
                     /gene="glcD"
                     /locus_tag="KJL94_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9245..10015
                     /gene="glcC"
                     /locus_tag="KJL94_000342"
     CDS             9245..10015
                     /gene="glcC"
                     /locus_tag="KJL94_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBD4408840.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10139..11280)
                     /locus_tag="KJL94_000343"
                     /pseudo
     CDS             complement(10139..11280)
                     /locus_tag="KJL94_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11642..13372
                     /locus_tag="KJL94_000344"
     CDS             11642..13372
                     /locus_tag="KJL94_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBD4408841.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13369..14283
                     /locus_tag="KJL94_000345"
     CDS             13369..14283
                     /locus_tag="KJL94_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBD4408842.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14316..14564
                     /locus_tag="KJL94_000346"
     CDS             14316..14564
                     /locus_tag="KJL94_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD4408843.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14564..15735
                     /locus_tag="KJL94_000347"
                     /pseudo
     CDS             14564..15735
                     /locus_tag="KJL94_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15771..16850)
                     /gene="lptG"
                     /locus_tag="KJL94_000348"
     CDS             complement(15771..16850)
                     /gene="lptG"
                     /locus_tag="KJL94_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD4408844.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16847..17917)
                     /gene="lptF"
                     /locus_tag="KJL94_000349"
     CDS             complement(16847..17917)
                     /gene="lptF"
                     /locus_tag="KJL94_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD4408845.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(17948..18508)
                     /locus_tag="KJL94_000350"
     CDS             complement(17948..18508)
                     /locus_tag="KJL94_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD4408846.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18520..19344)
                     /locus_tag="KJL94_000351"
     CDS             complement(18520..19344)
                     /locus_tag="KJL94_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408847.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19344..20693)
                     /locus_tag="KJL94_000352"
     CDS             complement(19344..20693)
                     /locus_tag="KJL94_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBD4408848.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20739..21497)
                     /locus_tag="KJL94_000353"
     CDS             complement(20739..21497)
                     /locus_tag="KJL94_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408849.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21529..22242)
                     /locus_tag="KJL94_000354"
     CDS             complement(21529..22242)
                     /locus_tag="KJL94_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD4408850.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22416..23108
                     /locus_tag="KJL94_000355"
     CDS             22416..23108
                     /locus_tag="KJL94_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD4408851.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23157..24656)
                     /gene="pitB"
                     /locus_tag="KJL94_000356"
     CDS             complement(23157..24656)
                     /gene="pitB"
                     /locus_tag="KJL94_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBD4408852.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(24948..26807)
                     /gene="gss"
                     /locus_tag="KJL94_000357"
     CDS             complement(24948..26807)
                     /gene="gss"
                     /locus_tag="KJL94_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBD4408853.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27012..27878
                     /gene="yghU"
                     /locus_tag="KJL94_000358"
     CDS             27012..27878
                     /gene="yghU"
                     /locus_tag="KJL94_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBD4408854.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHELHDAS
                     DFETNTEDKRQG"
     gene            complement(28001..28249)
                     /gene="hybG"
                     /locus_tag="KJL94_000359"
     CDS             complement(28001..28249)
                     /gene="hybG"
                     /locus_tag="KJL94_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBD4408855.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28383..28724)
                     /gene="hypA"
                     /locus_tag="KJL94_000360"
     CDS             complement(28383..28724)
                     /gene="hypA"
                     /locus_tag="KJL94_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD4408856.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28717..29205)
                     /gene="hybE"
                     /locus_tag="KJL94_000361"
     CDS             complement(28717..29205)
                     /gene="hybE"
                     /locus_tag="KJL94_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBD4408857.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29198..29692)
                     /locus_tag="KJL94_000362"
     CDS             complement(29198..29692)
                     /locus_tag="KJL94_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBD4408858.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29692..31395)
                     /gene="hybC"
                     /locus_tag="KJL94_000363"
     CDS             complement(29692..31395)
                     /gene="hybC"
                     /locus_tag="KJL94_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBD4408859.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31392..32570)
                     /gene="hybB"
                     /locus_tag="KJL94_000364"
     CDS             complement(31392..32570)
                     /gene="hybB"
                     /locus_tag="KJL94_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBD4408860.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32560..33546)
                     /gene="hybA"
                     /locus_tag="KJL94_000365"
     CDS             complement(32560..33546)
                     /gene="hybA"
                     /locus_tag="KJL94_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBD4408861.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33549..34667)
                     /gene="hybO"
                     /locus_tag="KJL94_000366"
     CDS             complement(33549..34667)
                     /gene="hybO"
                     /locus_tag="KJL94_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBD4408862.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34856..35143)
                     /locus_tag="KJL94_000367"
     CDS             complement(34856..35143)
                     /locus_tag="KJL94_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBD4408863.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35262..36149)
                     /locus_tag="KJL94_000368"
     CDS             complement(35262..36149)
                     /locus_tag="KJL94_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD4408864.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36306..37346
                     /gene="gpr"
                     /locus_tag="KJL94_000369"
     CDS             36306..37346
                     /gene="gpr"
                     /locus_tag="KJL94_000369"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBD4408865.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37386..37880)
                     /locus_tag="KJL94_000370"
     CDS             complement(37386..37880)
                     /locus_tag="KJL94_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBD4408866.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38071..38955
                     /gene="yghA"
                     /locus_tag="KJL94_000371"
     CDS             38071..38955
                     /gene="yghA"
                     /locus_tag="KJL94_000371"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD4408867.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39227..39652)
                     /gene="exbD"
                     /locus_tag="KJL94_000372"
     CDS             complement(39227..39652)
                     /gene="exbD"
                     /locus_tag="KJL94_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBD4408868.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39659..40393)
                     /gene="exbB"
                     /locus_tag="KJL94_000373"
     CDS             complement(39659..40393)
                     /gene="exbB"
                     /locus_tag="KJL94_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD4408869.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40645..41832
                     /gene="metC"
                     /locus_tag="KJL94_000374"
     CDS             40645..41832
                     /gene="metC"
                     /locus_tag="KJL94_000374"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBD4408870.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            41972..42631
                     /locus_tag="KJL94_000375"
     CDS             41972..42631
                     /locus_tag="KJL94_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4408871.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42671..43570)
                     /gene="yqhC"
                     /locus_tag="KJL94_000376"
     CDS             complement(42671..43570)
                     /gene="yqhC"
                     /locus_tag="KJL94_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBD4408872.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43764..44927
                     /gene="yqhD"
                     /locus_tag="KJL94_000377"
     CDS             43764..44927
                     /gene="yqhD"
                     /locus_tag="KJL94_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBD4408873.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45032..45859
                     /gene="dkgA"
                     /locus_tag="KJL94_000378"
     CDS             45032..45859
                     /gene="dkgA"
                     /locus_tag="KJL94_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBD4408874.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46059..46985
                     /locus_tag="KJL94_000379"
     CDS             46059..46985
                     /locus_tag="KJL94_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBD4408875.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47036..47293
                     /gene="yqhH"
                     /locus_tag="KJL94_000380"
     CDS             47036..47293
                     /gene="yqhH"
                     /locus_tag="KJL94_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBD4408876.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47336..49555)
                     /locus_tag="KJL94_000381"
     CDS             complement(47336..49555)
                     /locus_tag="KJL94_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBD4408877.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49666..51078)
                     /gene="ftsP"
                     /locus_tag="KJL94_000382"
     CDS             complement(49666..51078)
                     /gene="ftsP"
                     /locus_tag="KJL94_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBD4408878.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51153..51890)
                     /gene="plsC"
                     /locus_tag="KJL94_000383"
                     /gene_synonym="parF"
     CDS             complement(51153..51890)
                     /gene="plsC"
                     /locus_tag="KJL94_000383"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBD4408879.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52124..54382)
                     /gene="parC"
                     /locus_tag="KJL94_000384"
     CDS             complement(52124..54382)
                     /gene="parC"
                     /locus_tag="KJL94_000384"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBD4408880.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(54928..55410)
                     /locus_tag="KJL94_000385"
     CDS             complement(54928..55410)
                     /locus_tag="KJL94_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBD4408881.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55463..55855)
                     /gene="ygiW"
                     /locus_tag="KJL94_000386"
     CDS             complement(55463..55855)
                     /gene="ygiW"
                     /locus_tag="KJL94_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBD4408882.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56007..56666
                     /gene="qseB"
                     /locus_tag="KJL94_000387"
     CDS             56007..56666
                     /gene="qseB"
                     /locus_tag="KJL94_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBD4408883.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56663..57937
                     /gene="qseC"
                     /locus_tag="KJL94_000388"
     CDS             56663..57937
                     /gene="qseC"
                     /locus_tag="KJL94_000388"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBD4408884.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(57983..58315)
                     /locus_tag="KJL94_000389"
     CDS             complement(57983..58315)
                     /locus_tag="KJL94_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBD4408885.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58634..59215
                     /gene="mdaB"
                     /locus_tag="KJL94_000390"
     CDS             58634..59215
                     /gene="mdaB"
                     /locus_tag="KJL94_000390"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBD4408886.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59246..59560
                     /locus_tag="KJL94_000391"
     CDS             59246..59560
                     /locus_tag="KJL94_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBD4408887.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59608..61500)
                     /gene="parE"
                     /locus_tag="KJL94_000392"
     CDS             complement(59608..61500)
                     /gene="parE"
                     /locus_tag="KJL94_000392"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBD4408888.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61529..62110)
                     /gene="yqiA"
                     /locus_tag="KJL94_000393"
     CDS             complement(61529..62110)
                     /gene="yqiA"
                     /locus_tag="KJL94_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBD4408889.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62110..62937)
                     /gene="cpdA"
                     /locus_tag="KJL94_000394"
     CDS             complement(62110..62937)
                     /gene="cpdA"
                     /locus_tag="KJL94_000394"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBD4408890.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(62962..63384)
                     /locus_tag="KJL94_000395"
     CDS             complement(62962..63384)
                     /locus_tag="KJL94_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBD4408891.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63385..64014)
                     /gene="nudF"
                     /locus_tag="KJL94_000396"
     CDS             complement(63385..64014)
                     /gene="nudF"
                     /locus_tag="KJL94_000396"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBD4408892.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64219..65700
                     /gene="tolC"
                     /locus_tag="KJL94_000397"
     CDS             64219..65700
                     /gene="tolC"
                     /locus_tag="KJL94_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBD4408893.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65848..66519
                     /locus_tag="KJL94_000398"
     CDS             65848..66519
                     /locus_tag="KJL94_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBD4408894.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66525..67685
                     /locus_tag="KJL94_000399"
     CDS             66525..67685
                     /locus_tag="KJL94_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD4408895.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67723..68511)
                     /gene="ygiD"
                     /locus_tag="KJL94_000400"
     CDS             complement(67723..68511)
                     /gene="ygiD"
                     /locus_tag="KJL94_000400"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBD4408896.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68654..69427
                     /gene="zupT"
                     /locus_tag="KJL94_000401"
     CDS             68654..69427
                     /gene="zupT"
                     /locus_tag="KJL94_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBD4408897.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69485..69655)
                     /locus_tag="KJL94_000402"
     CDS             complement(69485..69655)
                     /locus_tag="KJL94_000402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408898.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69916..70569)
                     /gene="ribB"
                     /locus_tag="KJL94_000403"
     CDS             complement(69916..70569)
                     /gene="ribB"
                     /locus_tag="KJL94_000403"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBD4408899.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70673..70821)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            70943..71233
                     /gene="ubiK"
                     /locus_tag="KJL94_000404"
     CDS             70943..71233
                     /gene="ubiK"
                     /locus_tag="KJL94_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBD4408900.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17803 a        19038 c        17650 g        16917 t
ORIGIN      
        1 acaatatttg gcattacgat ttaatgcttc ataatcacca aattatttaa ccataaattt
       61 tcttatttct aacaaaatga ttggtgtgtt gttggagtta aaatacatac ataatattaa
      121 catctgccaa tatttcactt tccgcaagac ggatgatttc gaatcctcat ccacgttacc
      181 ctatcccaaa tatctttatt aataggcagt aacgaacact gttatagcaa gattaactta
      241 ttaacctaac ggcatctttt atgtaacaag tggagataaa ttgcttaaaa ttgatcctcg
      301 acaaatctta aaagttctca ttattctgca aaatcagctt tgcgaataat cttatgtttt
      361 tgggagtttt gggaattttg atctactcat tgttctagtg aaaaatataa ataagagaaa
      421 aaacgccatt cgtcatgaaa gcaacagatg aagcaatgta ataacatact aaaattgatc
      481 tcatgaaagt gggtacttta taaccagcac agaagccaag ttgtataaat attaaacaat
      541 tcgcatcgta aaacggagac tacgtataaa gaagccaatc gtaaaaacgg ttccctggcg
      601 agataaacga cagacaaatg gaaagccggg tgatatttac cttcatccgg ctttattgat
      661 ttacgagact aacatcccgg taaacacata cgcctgcagc agggtgataa cgccgataac
      721 gctggcaaaa atcagactgt gcttcacggt gtagcggaac agttcagatt ctcggcccac
      781 catgcccgtc gcggcgcagg ccacggcgat agattgcggg gagatcatct tgccagttac
      841 gccgccgctg gtatttgctg ccaccagcag agtgtcagag acgttgattt gctgcgccgt
      901 ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg tccgacccgg taaggaatac
      961 gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc acgcctgtac ctgccagtac
     1021 cagcgccagc gtggtggaca tgccggaata gttggtgacg aaggcgaatg ccagcaccat
     1081 gccaatcgac aatatcggcc acttcagact aattagcatt tcggcaaaga cgccaatccc
     1141 tttcttgatg cccacgccga ggatgaagat agagataatc gccgcaataa aaatagcggt
     1201 gccgccagcc gagagagggt cgaatttaaa caccgcatcc attggcgttg gttgggcgac
     1261 aatgggtgcc gctttcaaca cttgttgatg caaatgaggg atctggaaat taatcaccag
     1321 tgaataaaac gcgccgcccg gagcaaataa cgctttaaac ggcttcatgg tccagatggt
     1381 gaccagcacc gttaagatta aaaacggtga ccacgcacga atgatttgcc ccagactata
     1441 ttctgaaggc acgggaccgc cagaagatgg cttatttacc accatcgcac ctgcggattg
     1501 ccccatgctg attgccgttt cggtattttt cggccgccag actttaagaa ataaagcgag
     1561 tgagacgata ctcaccagcg ccgaagtaat atccggcagt tccggaccaa tatagttaga
     1621 ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc agcgccgctg gccacgtctc
     1681 tttcacccct ttccagccgt ccatcattgc taccagccag aacggcacaa gaaccgacag
     1741 gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac ggatcgattc ccgttacctg
     1801 accggcgacc agaatcggca cgcccaacgc accaaacgcc accgtcgcac caaagccagc
     1861 agctccttcc agcaacgcac caaaggagaa accaatcagt aacacctgca aacgctgatc
     1921 gtcggtgatg gagataacca agctgcggat aatatcgaac tgcccgctgg caacggttaa
     1981 tttatacagg aacaccgccg cgacaataat ccacgcaatt ggccataatc cataaataaa
     2041 gccatagccc gcagcagcaa atgccatatc aatcggcatt ttaaaggcga atatcgcaat
     2101 caggatagat aatataaggg ttattgctcc agcgacatgt cctttcagac gtaataccgc
     2161 gagtgcaacg aagaagaata ttatcgggat cagggcgacc agagcggata gccccagtcc
     2221 tcccatcggc atatacattt gggtccaggt aaccatattg ttcagtctct tattatcttt
     2281 atatgcttga tatacttaag gttgtaataa gcaaaagagg actgaactgt aaaatatagg
     2341 cgttatactt tacagcaaca gtacgccgct aacgcaattg ctacctctgg cataacaagt
     2401 atatcgggta agggtttctg ttccgcacac gcaggcgcag agtatcgtta agatgtccat
     2461 attgttgttt taggcccgct agtaatgcgc tacgggtatt taatattgtt aaaccctgat
     2521 aatcgctccg gttatttccg ggataaatgt actaccgcag ttactatcat agccccgaca
     2581 ataaaacttg ccggggcttt tttgacgcta ttaatgactt tctttttcgc gtaaacgcca
     2641 ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc acgccgagga agattaaatc
     2701 gctggcagct ttaaaagcac acgagttagc gaaattcccc gccatcggac gataagccgg
     2761 atcgccagca ttttgctgat caaccacttt cgccatattc tccagcgacg cctgcacctg
     2821 ttctttggtc agaataccgt gacgtaacca gttggcgata tgctggctgg agatacgcag
     2881 cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc ggcacttttg aacaaccaat
     2941 cccctgctcc acccaacgca ccacgtaccc cagaatcccc tgcacgttgt tatccagctc
     3001 ttgttggatc tcttgcgccg accagttagc gttttcagcc accggaatag tcagcagatc
     3061 gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg gcaatgttgg cttgcacgct
     3121 ctgtacgttg gtatggtggt agtgcagcgc atggagcgta gcagcggttg gtgacggaac
     3181 ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc tggctgtaca tgtctgccat
     3241 caggtccggc attgcccaca tgcctttacc gatttgcgct ttaccgcgca gcccacagaa
     3301 cagaccggaa agcacgttat tacgttcgta ggctttgatc cacggcgtcg atttcatctg
     3361 gtttttacgc agcatcgggc cagcttccat caccgaatgc atttcatcgc cggtacggtc
     3421 gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc tgagcgatac agctacgcaa
     3481 gttcagcgag gtccggcgtt cttcatccat aatgcccatt ttcagggtat tcggtgccat
     3541 accgagcatt gtctcaatgc gggtaaacag tttgttggcg aacgccactt cctgcggacc
     3601 gtgcattttc ggtttcacaa tatagacgct gccagtgcgc gagtttttct gcacttttaa
     3661 atcatagagg gcaatcgcgc cagtcatgac gccatcaaga ataccttccg ggatttcatt
     3721 gccttcgctg tcccaaatca caggaatggt catcaaatga cccacgttgc ggataaacag
     3781 caacgagcgt ccgtgcagag aaatttcaga gccatcggcg gcggtgtact gacgatcgtc
     3841 attcagttta cgtacgattt gccgaccgtt tttctccatt ttctcttgca gagtcccctg
     3901 catcaggccc agcagattgc ggtacagcag gattttatct tccgcatcaa ccgccgcgac
     3961 cgaatcttcg cagtcgagaa tggtactgat agcagcttcg acgataacat cgttgatgtg
     4021 cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt tgcagttcaa tatgcaggcc
     4081 gttatttttc agcaaaatgc aggtcggcgc agcggcatcg ccacggtaac cgacaaactg
     4141 tgctggagta cgtaacgtgg tttctttacc atttttcaac tggatgcgta attgtttatc
     4201 gaccacctta aacgccacca catcctgata gctgccgttt tccagcggta gtgattcatc
     4261 gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc tgcggatcgt agccactgac
     4321 catcgcccct tcctgcggga tgatgtcgct gccgtataac gcatcgtaca gtgagcccca
     4381 gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt gccggaacca ccagctgtgg
     4441 ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt tccaccgtca cgcgctccgg
     4501 ttgcggcacc aggtagccca gttcacgaag gaaagattta taggccgctt tatcttttac
     4561 cggccccgga ttgctgcgat gccactcatc aagcgccgcc tgaatgcgat cgcgttctgc
     4621 cagcaactga cgattttctg gtgccagatc gtgaacgatc tcatcaacat tgtgccagaa
     4681 tgcagcagcg tccagttcca cgcccggcaa aacttctcca tccacaaaac gtttaaaatt
     4741 ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc attgtttctc tcctcgtttt
     4801 cgcttatttc gccaacaccg ctgctgccac tttcgcgacc tgcgcatcct gtgctccggt
     4861 taaaccagaa acgcccacgg caccaataat ttgcccatca acaacaaccg gtacgccgcc
     4921 ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta cgtccgttgt tcaccatctc
     4981 ttcatagccc ttagtttcac gacgccccag cgcggcggta cgcgctttct cctgggagat
     5041 gtaagccgca atcggcgcgc aatcgtccat gcgacttaac gccagcagat gaccgccgtc
     5101 atcggcaaca gcaatggaaa cagaccagtt atttttctgc gcctcttcct gacctgcggc
     5161 aataattgca ctcgccattt gctggctaag aatgacttta gttttcattt ttttattcct
     5221 tttcaagggc ttgttctaca atttcaatcc agtgacgcac agaggtacga ccggcgctcg
     5281 ccagatgcgt ctggcaacca atgttggcgg tgacgatcat ttccggtttg ccgctttcca
     5341 gcgcattcat tttgttatcc cgcagctggc gtgccagatc gggatgcgtt aacgcatatg
     5401 ttcccgctga accgcagcac agatggctgt cgggaacgtc cgttaaggta aatccaagac
     5461 gaagtaacac tttttccact tcgccgttca gcttttgcgc atgttgtagg gtacacgggc
     5521 agtggaaggc cagtttttta tcgccgcgaa ttgccagttt ttccagcggt tcctcgcgca
     5581 gaagttcgac taaatcgacc gccagttcac tgacctgacg cgctttatcg gcatataacg
     5641 catcgttttt cagcatctgc ccatactctt tgacgaacgc gccgcaaccg ctggcggttt
     5701 gcaaaattgc ctcggcacct gcttcaatcg cgggccacca ggcatcaata ttattgcgcg
     5761 cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc caccgcgcca caacagcctg
     5821 cttcgttagc tgacatgacg ctgatcccca gacgatccag cactcgcgca gttgccgcgt
     5881 tggtgttggg cgaaagcgta ggctgggcgc agccttccag cattaaaacc cgacgcttat
     5941 ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc aggcagtttt gctctgacct
     6001 gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt cagcgcacgg aagaccgccg
     6061 gacgcggcac tacctggcgc aatccttcgc gcagcattcg ctccggcagt gggcgtttca
     6121 ctttctgctc gacaatatca cgcccgatat ccagcaaatt gtgatagcgc acaccagaag
     6181 gacaggtggt ttcacaattg cggcaagtga ggcagcgatc gagatgctcc tgtgttttaa
     6241 gcgtgacttc gttgccttcc agcacctgtt taatcagata gatgcgcccg cgcggcccgt
     6301 ccagttcatc gcccagaagc tgataggttg ggcaggttgc ggtacaaaat ccgcagtgaa
     6361 cacaggcgca caggatgctg tcggcttcca gcgcgcgttc tgccgcatct cttcggttaa
     6421 tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat gcgaccgggg ttaaacacgc
     6481 cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg gaataaagga gccgatagcg
     6541 gggcaaagcc accatctccg gcactaaagc gggtcgcatg accgccagcg ttgcgggcga
     6601 tgcgatggat ttgattgtca tcggctgtcg atttcagcca gcgtaacgcc ccgccccagt
     6661 cgatcagttg ctcgccgggt aaatccatca tcggcgcatc gctgggtaat gaaatgcgcc
     6721 ataaggtacc tggtaacgag aagaacggca gttgttgttc acgcaattgc tgccagaact
     6781 gacccgcaac ctcttcgcca cccagcagtt cacgcgctgc ttttaccgat ccttcgccgc
     6841 cctcaaggcg gatccacaac gcattgtcga agtaacataa gccactaatg ggtaatggct
     6901 ggagttgcca ctcggcgatt tcactcatgg cttcttgcag gctgatttcc cgacgcaggc
     6961 tcagagaggc acgcggtcgc ggtaacactt tcattgagat ttcagtgagc acgccaagac
     7021 aaccatagct tccggccatt aaccgtgaga gatcgtatcc ggcaacgttt ttcatcactt
     7081 cgccaccaaa acgcagatgt tttccagtgc cggtaatgat gcgcgtgccg aggacaaaat
     7141 cgcggaccga accgctccac gggcgacgcg gccccgccag cccgcaggcg accatcccgc
     7201 cccaggtggc ttcttcacca taatgcggcg gctcgcaggg aagcatttgc ccggcgctct
     7261 ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg agcggttatc accagctcgg
     7321 tcgggtcgta attaacaatg ccgcgatgac aacgcacatc cagcgtttgc ccggtgactg
     7381 ggcgacctaa aaaggctttg ctattgctgc cctgaatcac cagcggcgtt ttatcgctaa
     7441 ttgcctgatt cacctgctcc agcagcgcct gactgtaatc acactcgcgt agcatcaaaa
     7501 gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca tgcatggcac caaattcagc
     7561 acagcggtgt agcgtgggaa tgtttttccc agggttcagc aaaccatcgg ggtcaaacgc
     7621 cgccttgacc gcatggaagg tcgtgatttc atcgctgttg aactgggcgc acatttgatt
     7681 gattttttct cgcccgatgc catgttcccc actgatgctg ccgccaactt caacgcagag
     7741 ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat tcaccaggtt cgttggcatc
     7801 gaaaaggatt aacgggtgca tgttgccatc tccggcatga aagacgttgg caacacgtaa
     7861 atcatattgc tgcgataaac gggcaatgcc ttccagtacg ccaggcaggg cgcgacgcgg
     7921 gatggtgcca tccatgcagt agtaatccgg ggagatacgt cctaccgccg ggaacgcatt
     7981 tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc tgtgccaaac ggacgtcagt
     8041 cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag tcttcctgta cgtcagactc
     8101 cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg acgggataac cggcatgaat
     8161 aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc tccagcccgc cggggataat
     8221 gccattggcg atgatgtcac caaccgcaag tccggctttt tctaccgagt caaagctggc
     8281 taacagaacc cgcgccacgg gcggcttcgg cagcagtttt accgtcactt cggtggtcac
     8341 gccgagcata ccttccgatc cggtgaacag cgccagcagg tcaaaaccag gtgaatccag
     8401 cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt tgcacttcaa ttttcagcag
     8461 gttatgtacg gtcagaccat atttcaggca gtggacgcca ccggcatttt ccgccacatt
     8521 gccgccaatt gaacaggcga tttgtgagga aggatccggc gcgtaataga gattatgcgg
     8581 cgcaacggcc taggagatcg ccaggttaca tacgcccggc tgcacgcgcg cgcggcgacc
     8641 aacggggtta atgtcgaggc tctctttaaa gcgcgccatc accaacaaca cacctttttc
     8701 cagcggcagc gcgccaccag aaagcccggt gcctgcacca cgggtcacca ccggtacacg
     8761 caggcgatgg cagacagcca gaatcgctgt cacctgttcc atttgcttag gcagaacaac
     8821 cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac tcgtaaggaa tgatctcctc
     8881 atcggtatgc aggatctcaa gtccagggac atgctcacgc agtgccatca gtaccgatgt
     8941 gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg tacaagatgc tcatgagtag
     9001 gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc gctgcttctg tgaacagtta
     9061 ttaagcgggc ttttcgtttt cgtctatctc tttagctacc ggtcagacca ttttttttcc
     9121 agctctgtga ccttgtcttg gttaacttaa tgttaaattg atgtaacata atcacttgcg
     9181 tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt cctacctgtg cacgaggtcc
     9241 gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt gcagagagta tcgaacggtt
     9301 aattatcgac ggcgtactga aggtcggtca gccgcttccc tcggaacgtc gactgtgtga
     9361 aaagctcggc ttctcacgct ccgcactgcg tgaagggctg accgtgctgc gcgggcgcgg
     9421 gattattgaa acggcgcagg gtcgcgattc tcatgtcgca cggcttaatc gggagcagga
     9481 caccagcccg ctgatccatc tgttcagtac gcagccgcga acgctgtacg atctgctcga
     9541 cgttcgcgca ttactggagg gcgaatcggc aaggctggcg gcaacgctgg gaacgcaggc
     9601 tgattttgtt gtgataaccc gctgttatga aaaaatgctc gccgccagtg agaaccacaa
     9661 agagatttcg ctgatcgaac atgcgcagtt ggatcacgct ttccatctcg ccatttgtca
     9721 ggcttctcac aatcaggtgc tggtgtttac gctgcaatca ttgaccgatc tgatgtttaa
     9781 ttcagtgttt gccagcgtaa ataatctcta ccatcgacca cagcaaaaaa agcagatcga
     9841 tcgccagcat gcgcggatct acaacgcggt gttgcagcgg ctgccgcacg tcgctcagcg
     9901 cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc cacgatatcg agctggaagg
     9961 ccaccatttg attcgctcgg cggtgccgct ggagatgaac aaggtgggaa tgtgagcaga
    10021 gttttaccct atccccaagg cgaggggacc gcccctgcac ttaacaggat ctggtgttaa
    10081 atctgttcat ctataccctc tcccgaatga ccgcccctgc gctaaacagg atctggtatt
    10141 aaatttgctt ttcatacaat cggtaacgct tgtacggctc cgccccaatg cgttccagca
    10201 tgttattcat gcctgtattg gtttcgagga tccatgacat ctccagcgca tcgatcttcc
    10261 ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag cgcaatcacc gggccgatgc
    10321 ggctgaactg atactcgtcg cgcacgccca tcagcggtac tcgcgcagtt cgcacaccgc
    10381 tgactttcaa acgccacagc aattttgccc agccgaaggg aaagagcgat ccgttcagat
    10441 cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac gcgcagggtg cagaatcaat
    10501 ctcagcgatg tagatcatat cgtccggcac cagatattta agttggtcgc ccatggtcgc
    10561 gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc tgccagccag agttgaaaat
    10621 ctcacgcagg atctgcatct cttcggcaaa ccgctgacga ttgatgcagc gaatggtcac
    10681 ctttttgcgc acctgatcca tcagtttttt tagcgccgga gagaaagtga gatcggttcg
    10741 ctgcatccac cacgccagta aatcaatgcc tttgtgataa cccagttgtt caatatgcgc
    10801 ggcataccac ggtttgccgt gtggcatcat cgcacagggt ggtgtgtcaa aaccttcaat
    10861 cagtaatccg ctttcctgat tgatattcag gctgaaaggc ccgctgatct tacttgcacc
    10921 ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg gcaaaatcct gcggatcatc
    10981 aatggcgtca atcatgccga aatgaccggt atcttcaccg taacgctcac ggtgcaaggt
    11041 atctatttgc gcggtaatac gcccaactat ctgccccgct ttttttgcca cccacgcctg
    11101 ccagatgata tggtccgtcc ccggattttt cgcagacaaa tgctcgttgc gttcaatgaa
    11161 taaagggggt atccagtttg gatcgtcggg ataaagtgat gacggaaaag cgataaatgc
    11221 cttaaggtca tttttattaa ttactttttc aattttaatc atcagcagat cgcattccat
    11281 atctctgtgg ataacaaata attaatggtg aaaaacaggt tgcaacagca acgagcggca
    11341 tcttaataat caactcaatg aatacaaacg acgaataaat agtattaatt aatgattatg
    11401 ataaggtcag aggctacaca gaaacgcctc aatgcctttt gtcaatttaa ggcgaccgga
    11461 caaggcttct tcttaatata ttcataaatt aaatatcatt cataataaaa acaaaaaact
    11521 taaactcatt atttaaatca atgttcattt tcacaccgat gaaaatacta tctcatttat
    11581 cttttatgcc tatataatcc accgcattat tgtcatttta tttttgatat ccaatttttt
    11641 cgtggtgtat atgtctaata aaatctttac gcattcccta cctatgcgct atgccgattt
    11701 tccaacgctg gttgatgctt tggactacgc cgctctgagt agagccggaa tgaattttta
    11761 tgacagacgt tgccaacttg aagatcaact ggaatatcag acattaaaaa cgcgtgccga
    11821 agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa ggcgaacgcg tggcgctgat
    11881 tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc tgtcagtatg ctggcttagt
    11941 cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg gattcctgga gcgccaaact
    12001 ccagggttta ctggcaagtt gccagcccgc agccattatc actggtgatg agtggttgcc
    12061 actggtcaat gccgcgacgc ataacaaccc cgaattacat gttttaagcc acgcttggtt
    12121 taaggcatta ccggaagccg atgttgcgct ccagcgtcca gttccgaacg atatcgccta
    12181 cctccagtac acctccggca gcacccgttt tccccgtggc gtcattatca cccatcgcga
    12241 agtgatggct aatctacgtg ctataagcca cgatggcatt aaattacgcc ctagcgaccg
    12301 ctgcgtctcc tggcagcctt tctaccatga tatgggactg gtcggctttc tcctgccccc
    12361 cgtcgccacg caactttcag tagattattt gcgcactcag gattttgcca tgcgtccttt
    12421 gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt tccgttgcgc cgccgtttgg
    12481 ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc gctgaactgg atctttcctg
    12541 ctggcgcgtc gctggtattg gtgctgagcc gatctccgca gaacaactcc atcaattcgc
    12601 tgaatgtttc cgtcaggtta actttgacaa taaaactttc atgccgtgct acggactggc
    12661 agaaaatgcg ctggctgtca gcttctctga tgaagcctcc ggggttgtgg ttaacgaagt
    12721 ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg ccgggtgcag agacacgcgc
    12781 cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa catggtattg aaatccgcaa
    12841 tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat atttgcatct ccggtcccag
    12901 tctgatgagc ggttactttg gcgaccaggt ttcgcaagac gagattgccg cgacgggctg
    12961 gttagacacc ggcgacctcg gttatctgct ggacggttat ctgtatgtca ccggacgcat
    13021 taaagatctg attattattc gtggccgtaa tatctggccg caggatattg aatatatagc
    13081 ggaacaggaa ccggaaattc attctggcga tgcgattgct tttgttaccg cccaggaaaa
    13141 aatcattttg cagatccagt gtcggatcag cgacgaagaa cgtcgcgggc agcttatcca
    13201 cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc gcggctatcg atctgttgcc
    13261 gccccacagt attccccgaa cgtcctccgg caagcctgcc cgtgcggaag cgaaaaaacg
    13321 ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa tccctggcat aaatcaaact
    13381 gtcgcggtga cgggcgctac cgggtttatc ggtaaatata ttatcgataa cctgctcgcc
    13441 cgcggctttc acgttcgcgc attgacgcgt actgcccgcg ctcacgtcaa tgataatctc
    13501 acctgggtgc gcggttcgct ggaagatact cattcactta gcgagctggt tgccggagcc
    13561 agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca aagaagagat tttcacccgc
    13621 tgtaacgttg acggcagcct gcgcctgatg caagcagcaa aagagagcgg cttttgccaa
    13681 cgttttctgt ttatctcttc gctggcggcg cgccatcccg agctctcctg gtatgcaaaa
    13741 tccaaatacg tcgccgagca acgactggca gcaatggcgg acgaaattac gctgggcgtt
    13801 tttcgcccga cagccgtgta tggtcccggc gataaagagt taaaaccgct gtttgactgg
    13861 atgctacgcg gcctgctgcc acgacttggt gcgccagata cacaactctc ttttcttcac
    13921 gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg ccgaaactgt acagacgcaa
    13981 acctatgaat tatgcgatgg cgtcgccggt ggctatgact ggcaacgcgt acgacagctt
    14041 gccgccaaag cccgttgtgg ttccgtgcga atggttgaca ttcctctgcc ggtactcaca
    14101 ggccttgcgg atatcagtac cgcgttgagt cgcctggcag gtaaagaacc tatgctgacc
    14161 cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg caagtaataa ccgtatttct
    14221 gaagatatca attggtttcc cgggattagc ctggaacacg cattacgcaa cgggctattt
    14281 tgattgtctt gttttaaagg tgtgttaacg tttctatggt aaatcgtgaa atagtaatgg
    14341 attacatcct ttcctgttta caggatttgg tcgaaaacgg tgtggaaatt aagcccgaca
    14401 gcgatctcgt caacgatctt ggcctggagt cgatcaaagt gatggatctc ttaatgatgc
    14461 tggaagacag attcgatatt tccattccta ttaatattct gctggatgtc aaaacccctg
    14521 cgcaattaat ggaaactcta cttccatggc tggagaataa ataatggggc tatacgataa
    14581 atatgcgcgc cttgctggcg agcgtctgca atttagtgac aacgggttaa caccgtttgg
    14641 cacctgtatt gatgaagtgt attccgccac cgaaggccgg atcggcaata aaaaagtgat
    14701 tctggccggg accaataatt atctcggttt aacctttaat catgatgcca tcagcgaagg
    14761 tcaggccgcg ttagcggccc aaggcaccgg caccaccggt tctcgtatgg caaacggcag
    14821 ttatgcgccg cacctggcgt tagagaaaga gattgccgag tttttcaatc tccctaccgc
    14881 tatcgttttt caaccggtta taccgctaat ttaggcgtca tcagcgcact tgccgaccat
    14941 aacgccgtgg tgttactgga tgccgacagc cacgccagta tttatgacgc ctgttctctc
    15001 ggtggcgcgg aaattattcg tttccgccat aacgatgcta aagatctgga acggcgcatg
    15061 gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg ttgagggcat ctacagcatg
    15121 ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct cggtggttat
    15181 ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg caacggggcg cggactggct
    15241 gaagctgtcg gcgttgaaga tgatgtggat attatcgttg gtactttcag taaaagtctg
    15301 gcctcgattg gcggttttgc tgtcggttcg gaagcgatgg aagtgctgcg ctacggcagc
    15361 cgcccgtata tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt gcgctcgtcg
    15421 ttaagaacta ttgccacaca accagagctg cggcaaaagc tgatggataa cgccaaccat
    15481 ctgtatgacg gtctgcaaaa actgggttat gagttaagtt cacatattag cccggtagtg
    15541 ccagtaataa tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct tatctcgccg
    15601 ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg caggcatcac gctgctgcgc
    15661 tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg ccatcattca ggcatttgct
    15721 actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt ttaatgttgt
    15781 ctgtcatacc aatacaccag gctcaacgcc accaacacaa acgctaccgg taaaccgagc
    15841 gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat taaccatgat ttgatagctg
    15901 atccaggtga gtaagcccac gatgacgcct acagcgagac ggctacccat cccaggcgaa
    15961 cgcggggcac taaaggtaaa cggcacagcc agcaaaatca tcgccagggt caggatgggt
    16021 tgccccagtt tttcccacag tgcaaggcgg tattcgctgc tgggttgccc ggtttcctgc
    16081 aaataatgga tgtaatgatt aagctgacgg acagaaaaag tattccccgg cattgataac
    16141 tcttcaagat tcatactggt gaaggccgat tgccaggcaa gattatctaa tgtttccagc
    16201 gtttctttgc cattcagcca ttttttatga ttcacaccat gcaggaccca cgttttgtcg
    16261 tcttcaatgg tggcactgcg tgcataaatg taggattcaa gcgagagatc gtcgcgataa
    16321 tgaaatatct ccacgcccac cggctggccc tgctcgttca gcgatttcac cgtcacaaat
    16381 tcattaccac gcctggccca cagcatattg ccggtaatgt catcgtcctc ccccaacgcg
    16441 gtggcggtcg atttgatttg caacgcctgt tgctgcaatg gcgacgccac ccactcatct
    16501 atcgcgccta acgaaacagt ccacaatatc ccggcaacca gcgccaccag tgcaatacgg
    16561 aagatagaaa accccgtgct gcgaatggcg gtaagctcac tgtttttcga taactgcccc
    16621 agtccaacaa taccacccaa taacgcgata aacggcgaaa gttcgaccag tgtgcgcggt
    16681 aaggtcatta gcaccaccag caccgcctga gtccagcgat aaccgcccgg gctgacacca
    16741 tccagttcgt taatcaggtt gaaggtggta aaaagcggca gcaatagccc tgcagcggcg
    16801 gcaaaaccga gaaagagatg acggattaaa tagcgactga aaacattcat cgggaaaatt
    16861 tctgcagcaa agagaagtca cgtgcgacca gtatcagcag gcccattagc atgagtccgg
    16921 gcactaacca caaaccagga atgaggggaa tcgcgccgtt agccaccagt gtacggcaga
    16981 cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag tggcaataac gttgaaaatc
    17041 gcccttgccg cggttttacc cgacttaatg aaatggccag cagcgccatc aacaatgcgg
    17101 tcaggccacg gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag gcggagcgcg
    17161 ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc agcggattca
    17221 gatgtaattg cagattacgg tagatctgtt cgttgtcgtc acgcccctga tgatcgagaa
    17281 gataggcagt gccgttgtgc aacataacgg tcggtttctc gggtgatggg tcaaccacat
    17341 caaccgaacc ggcgcggaaa atacgggttc gattggcagt agaagtataa atcagcgcgt
    17401 cagtcaggcg attattatcc tgatcaaccg tctgcgaaag gatcattcgt ccgttatcgt
    17461 tagtgttaaa tttctttgcc cgcaactggc gaacatccag ctccgactgc gactgctgct
    17521 ccagttgata aatctgcgca taagcccacg gacgaccata catcgacaga agggtgacaa
    17581 aaatacttag cggtatcgcc agataaagaa cggctttgta caagcgtccc ggactgcccc
    17641 ccgccgcaga gatagcggta atttccgagt cggtatacat ctgccctagc gtcacgccaa
    17701 ctgacacata cagaccaaca ggtaacaaca tctccagtgc aatcagtact ttgtagaaaa
    17761 cgatatccag cacaacatca agagctaatg tcccatttgc cgcttcggtc agataacgct
    17821 gtgcggagta gctggcgaaa ataaagatca gaaaaccgac gataatcagc accagacggc
    17881 gcgtaccacg catgatgtag tgttcaacca gcttcatctc actaacgccc tcaatccttt
    17941 gagtttttta ttcattgatc agtttctgcg ccaacgcaat gacatgatct acctcttccg
    18001 gcgtcaggct gccgtccttt gcccactgaa tttgcccggc tttattcagc accagaatgg
    18061 ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg tccattgccg tcaataacga
    18121 attgcgccca cggatagcgg cgtttatttt tttcgatttt gccaacaaca aaatagccag
    18181 taccaaagat ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga tcctgcggaa
    18241 agtttgccgc ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc gacttacgcc
    18301 cggcgatata ctgaataatg cggacttttc ccgctaattg tgtgctgttc cacttttggt
    18361 agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata taaacagggg
    18421 taaccgtttt gccagtgacg aaattgtgtg cctgcacacc cggggctaag agcatgataa
    18481 tcaacgcaat aatcaaacga gatgacatca ttgctccggc tatagcggaa aacgcgaaat
    18541 cagcagctct aagcgctgac aaatatcgct atccagcgcc aacggtgcat aatacgactg
    18601 gcggttttcc atatcatccg ccaccatgtc gtcgtgttta tctcccatca ggccattaat
    18661 ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa gcgatcttaa
    18721 ggtgtcgtaa taagaaacct gctggctgat gatttttcgc acatcatgaa attccctgcc
    18781 ggttagtcgc ggtttggcaa taatctcttt cagggtatgc atttgctttt gccgataggc
    18841 aataaaactg cttacgcttt ctaactggct atggcgtaat gaatagttca ccaacgacca
    18901 gaccggagag cttaaataaa tccgtaacag gttgccgtaa tacgacacaa tatttttctt
    18961 accgttacga aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc cgaataaaaa
    19021 tccggcctga tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct tgtagcgcgc
    19081 tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt tggcttgttt
    19141 gcgaactaaa cgcagtaatt cctgacgatc aacgccattg agccagaatt gcaggcataa
    19201 cgcgtaatta tcatgaatga tttcggtggt acaacttagt gcaatagtcg gcggcaaact
    19261 aacctgtgca tcaacgacat catcttcttc aatcgcaaca aatagacgct cgctcgcctc
    19321 aagcgggaaa tattttctca tcatcaggat ttctctccgg ctgcgttata aagcaaggaa
    19381 tggcgctgaa aaaatgcctt taaggttgga attaacgaga gcgccacgtc cagacattgc
    19441 ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca gatgacagaa catgaacagc
    19501 aatacgatgt agccgattga ggcgatggtt tgcgccacga gaaaagcgcg ctgttttccc
    19561 gccatcaata gcaatgactc ctgcgggaag cccagcatcg aaatcacaat tgcgcccagc
    19621 atcacctgaa tcaggtcata cgcttcgaga tacttaacgc caaacaccag cgaaatgagc
    19681 ggcttgccga cgatcaacac cgcgagagcc accagaatac cgattccgcc cgccagtaaa
    19741 ccggacttca cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg catcacctct
    19801 gggtaaaaac ttttacccag caaacccgct ggcgttccgg cggcgtcgaa gaatgtcatg
    19861 gcgattttaa ataacccggc ggcagcgggt cctaacacga tccccaccaa cactgtgctg
    19921 cacgagttac gcgccgacca gatggagtgg gcaatgttgg ttgaccagac aaaactccac
    19981 gcgcctttaa tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt atggatattt
    20041 ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac cagattcgac
    20101 acgtaccagg caataacaaa acccgcaaaa ccaaagtcaa aataccaggc tgcgacgctc
    20161 cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc aaagcgatct
    20221 accgcacgca gaatgccggt cggcgtggag gaagccattg aaggaatgag cgtgcaatac
    20281 agcgccgcca gccaaaagct ttggtcatcc agacctaatg aatgggaaag gaatggcagt
    20341 aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga tatccagcga gaaggaaaat
    20401 gagacgacat tgcgaaattg ctgcggatta ttgttggtca aggctggcgt tccgtactga
    20461 accaccagct gccatgtctg aaacttaata aaatcgctga tcgacttggc gtacgattgc
    20521 acaatcacca gtacaccaaa catggcgggc gtcatccctt taccggcaca cgagagcgcc
    20581 aacagcccca gcaaggcgct ctcgacatta ctggagccta accaggcgct attgcgaata
    20641 atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc caattcagcc
    20701 tgaccttatc gttgatagta aaaaatatcc cgccagcctt aagttaaact tcggcggtca
    20761 gaaacgatgg caaccagaga aaccgccttc tgtacctgtt ccagcacttc gctgtagggc
    20821 gctctggaat caatctcaag aatttttgtg ccgttatagc caatcgtcga catgacgccg
    20881 attttgtcct gcagctcggc atactcatgg tcaggcttgc gggagatggc agtatcaata
    20941 tcaatcccca aacgaatgat taattcagga cggtattgcg ccattttttg gtataaactc
    21001 cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg cacgttcgac gccgatcccc
    21061 ggtccgtcat aataaaagcc cgaaatttca gcctgcggga agcgatcgct gaccaccaga
    21121 acgccacttt gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg cagcgagaag
    21181 cagtacataa tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct ttgggttttc
    21241 gaggatttgg ccgccaatcg ccgttccagc cagacgccaa ccaacggcaa tcgtttgatt
    21301 ttgtcgccgt cttcgccgga gagcagcccc agatagcggc gctcggtttg ccagtgttgt
    21361 tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac cggtgccatc acacccgacc
    21421 accgcaatca gccccggaat gtagttgggc tgcggcgcgg ttgtactatt aacagtttgt
    21481 gtttgtagtg catccattcg ttaaatcatc ccctatccat tttggtgatc aggaaagcgc
    21541 ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc gaccatccag
    21601 ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta acggcgattt tttcctgtaa
    21661 cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa tgccaagacg
    21721 aatcaacaat acgggtaaat aagacgccat ccattggtac agcttcaact cgcgctgcct
    21781 taacattttt atccaaccgt taccgcccgt ggtttttgcc aattgcgggc catcaaagcg
    21841 aaaacccggc acttcaacct gcgggtagcg gtcggtgatg agcagatagc cttgctggct
    21901 tttacacaac attttgcgaa acttgtacgc ccgccagcag gaaagcagat agataaccag
    21961 cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt acatgtgccg ctttacttcg
    22021 cagataacac ccacaaggtg cgccaataac agggagctgt gaaatccatt cgccaattcg
    22081 cccggacgat tgcccgagat aaatgtgttc tgttggcatt cttgctgcca gttcatttac
    22141 caggcttgcc gtgagggtcg atttacctga accatcacat cctacaatgg caataacacg
    22201 cactggcgtg gaattaatta ttgacatatt acgttgattc acgaaaaacc cggcagtaaa
    22261 taatgtattg aatattacgt ggtccgtgct cagaatatcc gttcaacctt attgtgacaa
    22321 tggctaattg tgacattgac ctgaattacg ctttcataaa aacatattaa ccaaataaat
    22381 atttttaatg gatatttaaa ttaaaggata tattcatgca gtcaataaca cctccattaa
    22441 ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat cttattaccg
    22501 ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc ggaaatgtcg
    22561 gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt acataaaaca attgaacgaa
    22621 atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc ggcgctggta attacagcgt
    22681 ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt cgcgggttaa
    22741 ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat ggtacggtgt
    22801 tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag gaacgtaaag
    22861 cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat gttgaccttg
    22921 aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg aagattgcca
    22981 taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc aatcagccac
    23041 tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc gcgcgtggtt
    23101 atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca ggaaggttaa
    23161 atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc cggtaaagta
    23221 aacacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc gccgtccacc
    23281 accatcgtcc ctgctaccgc agacgagagg acgtgtgttg tggagacagg catcccaata
    23341 taactggcaa ggccgataga cactgccgcc gtcatttgtg ccgccatgcc ttgcgcatac
    23401 gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg ccagccaatc
    23461 atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg agcgtactca
    23521 atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc tttactgacg
    23581 cctggcagtt tcgccagctt cgcggaggta tcggagatgc acagcataat gcggcgcagc
    23641 tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg cagcatcgtt
    23701 ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc tgttacttgc
    23761 gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt ctgcggcagt
    23821 tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt acgggtaatt
    23881 tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc caccagtacc
    23941 agcattacca ggccgatccc tttctgcccg tcgttcgcgc cgtgcgaaaa cgccacgccc
    24001 gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt gccctttttc
    24061 tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc actccagaaa
    24121 cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg ggaaacaatc
    24181 agcgaggaga aaattttggt tacttcacgc aggtttaacg catccatcac cgatgagccg
    24241 gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt gtgcgaactg
    24301 gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc cagcagcatg
    24361 gaaaagacca tcgccaaacc gtgagttgac cccatattca gcaacaaatc gattggtaac
    24421 atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa aaagttaaaa
    24481 aatgccgcca tcaccacagc aagttgtggc tgcatggcac gagtataaat aacggttgcc
    24541 accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac aaatgccaga
    24601 gcaagtaata acaaaagccc cgtatatata tcaaggccaa caaataaatt tagcatatat
    24661 tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta acggcatggg
    24721 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
    24781 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
    24841 ttcacatatg aaatgaataa atattggaat atataaatat tgaatatttt gattaatccc
    24901 cgtactaatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
    24961 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
    25021 cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta tttaccgtcc
    25081 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
    25141 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
    25201 gcgatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
    25261 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
    25321 ccggggatca ccgtccacag cggctcaaag accagcactt ccgggcgcag caatacgtcg
    25381 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
    25441 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
    25501 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
    25561 agcccgcgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
    25621 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
    25681 ctgtgtttcc aggcaccagc cagttcgtta atcagccctt ccgccggatt gaagccgttg
    25741 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
    25801 gcggagtcgg cgttgtattc gtaaaccttc aggccacgct catccatgca gaaatccata
    25861 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
    25921 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg
    25981 tgaagataca tcagatgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
    26041 gtaatggtgt agtaatgata cggatcctga ttgatcacct gaccgttggc ctgcacatag
    26101 gcgttttgta gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
    26161 agacgcgctc cactgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
    26221 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtcttta
    26281 agggtatagc agccgttttc gaccaccatc tccagctcgc gcgtccactg ttgcccttgc
    26341 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
    26401 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
    26461 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
    26521 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
    26581 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
    26641 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
    26701 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
    26761 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
    26821 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
    26881 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
    26941 aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa
    27001 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
    27061 ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc tggtccgacg catgaaaaaa
    27121 cgctgcccgt tggcaaacat ccattgcaac tttattcgct gggaacgccg aacggtcaga
    27181 aagtaacgat tatgcttgag gagctactgg cgctgggcgt tactggtgca gagtacgacg
    27241 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
    27301 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg
    27361 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatc
    27421 tggcaaaacg gactgaaacg ttgaactggc tgttctggtt acagggtgcc gcgccgttcc
    27481 tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag tacgccatca
    27541 accgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa ctggcgcagc
    27601 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
    27661 gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg ggaagttata
    27721 aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaaa cgtgggcgta
    27781 ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagctccat gacgccagtg
    27841 attttgagac aaatacagaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
    27901 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
    27961 cgacgctgcc gcgtcttatc aggcctacaa aagctaatca tcacgcgctg gtaatgtcgt
    28021 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
    28081 tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg ttaccttcgc
    28141 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
    28201 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt gttaactccg
    28261 gttatttcga ttgccggaca aacgcgaaaa agaaccttta gcgggtgttc cctctcaccc
    28321 taaccctctc cccggagggg cgaggggacc gtccgtgcgc tttttaggcg ttgtcctcag
    28381 tcttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac acgcaaccgc
    28441 tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac ctggctgcaa
    28501 tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc ttgcgccacc
    28561 gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac gcaggagagc
    28621 gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc cgcctgccgt
    28681 tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc tcctcctgcg
    28741 gccaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattggtga ctggcaacga
    28801 aaggatcatt cgtgcgcagt catccgtcag gcgttggcct tcttcaatcg acatgctgtg
    28861 cgaaagcggc gacatcagcg aacaggagag gtattgcgaa acaccgtcca gttcgccaac
    28921 ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca ctttgcgcag
    28981 cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg gggtgatcac
    29041 acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg gcattgaagg
    29101 atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg ctgcctgtac
    29161 ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc gcctcccgtg
    29221 ggatggcttc cacgcctgat tcgcgcagcg cagccagaac ctgctcaagc gcaggttcaa
    29281 tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc gggatcacgc
    29341 cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac aatacgtcgg
    29401 ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact tcttcatccc
    29461 gcaggatcat catcgttccc ggagtgttct ttttcgacac aatggcatcc gcaataatca
    29521 gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg ccatcgagga
    29581 tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc cgcacgccga
    29641 tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc attacagaac
    29701 cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac aggccatgca
    29761 cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat cggcaaccgg
    29821 tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac gcggaccaga
    29881 gttccaggtt gatggaacaa ccgcctggta gttgctgatg ataccgtctt taataaccat
    29941 ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga actcacccgt
    30001 tgccggaatg ttcggcttca caaaggtggt gtgatcgcct ttgccgatat tggtgatcag
    30061 tgcactgtat tggttttgca ggatatcctg caattcacag cagtgaacgg tacgaccaat
    30121 aatacggccc agcgtggagt gcagctgtgc cacttccagc gtgttgccag tcagtttctg
    30181 ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg ccgccagttt
    30241 caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag tcggtgattt
    30301 cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg tggtaccttc
    30361 ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc ttcgcgcttt cctgaatgcc
    30421 tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca gatccgcatt
    30481 ctcaatgtag cctcccggga acaggaagct gccgtttttg ctgtcggtcg ggaattccgg
    30541 cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt agaacgcggc
    30601 gataaccgcg gtatcaacct tataaacctg ctcaacaaag tcgctcagtt tgtcgatgaa
    30661 agacttgatg tacatcaggc gctcaaggtt caacacgccc aacccgtcga ggttgattgg
    30721 gttcgcgaca ccacccactg ccaggttctg aatgtgcggc gttttaccgc ccagcagcgc
    30781 cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg ctaccgcaat
    30841 caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat tggcgaaaat
    30901 acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag tgaactcttc
    30961 cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt tggttgggtc
    31021 agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gaaagctgat agaaatgaac
    31081 gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt gcgcgttaac
    31141 cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg cgtgagtggt
    31201 agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat cgcggttttt
    31261 cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg aaacgacgcc
    31321 attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg gatcaatagt
    31381 aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg ttttaaagga
    31441 ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat agccacaaaa
    31501 ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc gcccgggttg
    31561 aatgccacca acgaataggt cagacgccag gttgcacaac ctaacagtgc gctcagtgct
    31621 gacaggaaca gcatgcggga atcattacgc agcttcgcca cacgcagaac gaccagcggg
    31681 aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc ggcaaacgct
    31741 aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcccgatgaa aatcgccagc
    31801 aacacactga tggtgttggt cagcttgacg aacaggctct tttcatccgg accgttgcca
    31861 cgcagacccg cctgcaccag tgaaccttca aagatgacaa tcgagaagcc catgatgaac
    31921 gccgtcagca gcgagaacag cggcaacatt tcatagctct gccagagcgg atgcaccttg
    31981 tagcccgccg agatcatcag cgaccccatt gaagactggt gcatggtcgg cagcagcgca
    32041 ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt gtagcgacac cttccagccc
    32101 agacgttcaa acagtgccgg agcaaactcc agagccatca cgccgatgta gatggtcata
    32161 cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat gtagaagtac
    32221 ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca agccacccag tgagtaacca
    32281 aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg gttaaagacg
    32341 tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccaat cagcaggtca
    32401 aacgcgatcc acacgcccca cgggaagccg ccgttcaggt cagagacaga gcccagaccg
    32461 aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc aaaaatcatg
    32521 accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat gatcgtctcc
    32581 tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc ccgccagcac
    32641 agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg aacgcgcacc
    32701 ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac ccgtcagtac
    32761 cagaacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat atttcggcac
    32821 cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt attcgctgcc
    32881 aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg taccgaaaat
    32941 caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat cgagacgttc
    33001 cacacctttc tggttgcaca gctcgcactt atgcagcgca ccaaacgggt tgttgtagtc
    33061 gtacttcggc acgttgtacg gacaggcgac catgcagtaa cggcaaccgg tgcacacgtc
    33121 tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agtgcagaga ccgggcacac
    33181 agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg cgtagccgtt
    33241 ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga tgttattggt
    33301 atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt tacgttcagg
    33361 gaagttgata tcctgacact tggtgacgca agcctggcag cctacgcaca aggtcgagtc
    33421 gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg catgactgac
    33481 agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa aattacgtct
    33541 gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg acccagttca
    33601 cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag caaaccaata
    33661 gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg tgaacgcgga
    33721 gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat acctttttcg
    33781 ttacagccat agcacgggtg accaatcgcc accggccaca caccgccaac atcgcagaat
    33841 tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg gtacaggcac
    33901 cagccttcgc ggtggccttc atcaccgaac tctttggcaa aacgaccagc atcgaagtgc
    33961 gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg acggtttttg
    34021 tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc gaggaagttg
    34081 tgcgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag aacttcttgc
    34141 aagctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca cgcggagcag
    34201 gaaccaatag cgataatggc tgctgagcct tccgccgctt tgcggatgtg atccacaatc
    34261 ggctcaccgg caaccatgca ataaataccg ttatctttta atgggatgga accatccacc
    34321 actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc ttcgacctga
    34381 tgaccgaagg cggcggaaag cacttcgtga tattccagag agatagtctc cagcacgagg
    34441 ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt gcactcctgc
    34501 gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc catctctgca
    34561 gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt catgaaatca
    34621 cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta tagttattcc
    34681 gttgcgagga cctggcatat attttgcctc aatcgcaaaa tcaataatgc gatcgatgcg
    34741 ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg ggcgctaaaa
    34801 cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg tttgatcaat
    34861 tttcgaggca ataaccggcc ataaaaaagc gcaacgtaga acaggaatta ttctcacgaa
    34921 agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata cccgcttccc
    34981 aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag cctaatacaa
    35041 aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg gcatgcgttg
    35101 cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac ctcattcgtt
    35161 atatatcgcc atatatagcg atattttagg caaagaacga cttttgcgca cgctattttt
    35221 tcatacttgt ttaagacgta atcccgtaag agataaacct atcaggagag atatttatcg
    35281 aaccatttca gtgtcctttg ccaggcaaga tcggcggcag atttgtcata acggggcgtg
    35341 gaatcattat ggaatccgtg attaaccccc ggatagatat aagcctcata aaccttatta
    35401 ttgtctttca acgccgcctc gtaaggaggc cagccctcgt tgattcgggt gtccagttcc
    35461 gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg tgcctgacga
    35521 ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc gttcgatacg
    35581 ccaccgccat agcaaaatcc ggtaataccc actttacctg tcgcttgcgg atagcgttgc
    35641 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    35701 agctcacgac ctttatcatc atttcccgga taacctccaa cggaacttaa tccgtcaggt
    35761 gccagggcga tataccccgc cttcgccact cgccgtgcca catcttcgat atacggattc
    35821 agtccacgat tctcatgcac cacccccacg gctggcgttt tgccgctcat ttttgcgggt
    35881 ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt gatgtactca
    35941 gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt tggcttgagc
    36001 aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat acttcgccgc aaggttgagg
    36061 aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt agtaatccaa caactcttgt
    36121 gggaaatctt tggcggttaa acgcggcatc gttgcattcc tcaactggtg tttttttaag
    36181 caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca gactacggga
    36241 tacgcgctgg cgaatcgcta aactagaaac attgtttcga aattgaacgg aggaaaggag
    36301 aggtcatggt ctggttagcg aatcccgaac gttacgggca gatgcagtac cgctattgcg
    36361 gaaaaagtgg tttacgcctg cccgcattat cgctcggttt atggcacaat ttcggtcacg
    36421 ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta ggcattacgc
    36481 actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag aactttggtc
    36541 gcctgctgcg cgaggatttt gctgcttatc gcgatgaact gattatctct accaaggctg
    36601 gctacgatat gtggcccggc ccgtacggct ctggcggttc acgtaaatac ctgctcgcca
    36661 gcctcgacca aagcctgaag cgtatggggc ttgagtatgt cgatatcttt tactctcatc
    36721 gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat gcggtacaaa
    36781 gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg caaaaaatgg
    36841 tcgagttgct gcacgagtgg aaaattccgc ttttaattca tcaaccttcg tacaatttac
    36901 tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac ggcgtgggct
    36961 gtattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc aacggcattc
    37021 cgcaagattc acggatgcat cgtgaaggga ataaagttcg tggtctgacg ccgaaaatgc
    37081 ttaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag cagcgtggac
    37141 aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat
    37201 tgattggtgc cagccgcgcg gagcaactag aggagaacgt gcaggcgctg aataatctga
    37261 catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc gagctgaatc
    37321 tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg gcgcgcgaac cgcgcccgaa
    37381 tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa tgcagaaagc
    37441 acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac atttttcgca
    37501 tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc tgctactttg
    37561 tttttcagcg acgttgcgtc cattttcccc agccacttca gcttctcttt gttctcggag
    37621 atatccagct gtgagacgaa attctcataa ccggaaaaca tcaccatcac cagcaaaccg
    37681 ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga ttccgccatc
    37741 gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc cagggcaact
    37801 aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc atacattgca
    37861 ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa cagtatatcg
    37921 caattgagta aacagcacgc aacagcaaat caacatatac gttaaacctt tgtcgcataa
    37981 ttctgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg tctaccctta
    38041 aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc gaccacgcag
    38101 tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca ggcgaagatg
    38161 acaccgatac cggattgcgg cgagaaaacc tatgttggta gcggtcgcct gaaagatcgt
    38221 aaagcactgg tgacaggggg cgattccgga ataggtcgcg ctgccgccat cgcttacgcg
    38281 cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga cgctcaggat
    38341 gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg cgatttaagc
    38401 gatgagaaat tcgcccgttc gctggttcac gaagcgcaca aggcgttagg cgggctggat
    38461 attatggcgc tggtcgccgg gaaacaggtt gccattccgg atattgcaga cctcaccagc
    38521 gaacagtttc aaaagacctt tgccattaac gttttcgcgc tgttctggct aacccaggaa
    38581 gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca cttcgtcaat ccaggcatac
    38641 cagccaagtc cgcatttact ggactatgcg gctacgaagg cggcgattct gaactacagc
    38701 cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc
    38761 cctatctgga cggcactgca aatttccggc ggacaaacgc aggataagat cccgcagttt
    38821 ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc tgtatatgtt
    38881 tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggcgt gtgcggcggc
    38941 gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca ttgtgaattc
    39001 actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa gattcaacat
    39061 gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag cacctaatgc
    39121 ctgatgcgtc gtgaacgcct tatccggcct acaaacgcac gaagattcaa catgttgcaa
    39181 tagattttgt aggcctgatg agcgtagcgc atcaggcaat tctactttac ttcgctttgg
    39241 cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc gtatccatta
    39301 ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt
    39361 tcttgccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg accggatcgt
    39421 taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt tccgggcgcg
    39481 gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc gctaacggtg
    39541 ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc gtcacgttga
    39601 tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc atcggacatt
    39661 atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg gcttccagat
    39721 ccaggtcacg gctttgcagc aacaatacct gcgctgcaac atcacccagc atcgctttaa
    39781 agccgccaat ctggcgtgca aatacgttat agataacgac cgcaggaatc gctgccacga
    39841 ggccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact gccaggttag
    39901 tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc gtaccaaaca
    39961 gaccaacgaa cggagaaatc gcgccgatgg ttgccagata gccgttaccg cggcccattt
    40021 gacgacccac tgcggcgacc cgacgctcca gacggaagct ggtacgttct ttaatacctt
    40081 cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg agcaaatgca
    40141 ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt aaggaacgtg
    40201 cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc
    40261 tcttactgaa gaagattgcc caggtgacta cagaggccaa aataagccca atcatcacgc
    40321 acttaacgac aatatcggcg tactgataca taccccagac ggaaaggtcc gtctgcatta
    40381 aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca taataatatc
    40441 aaaacgacgt cgaattgata gtcgttctca ttactatttg catactgccg tacctttgct
    40501 ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc
    40561 tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt agacatccag
    40621 acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca actggtgaat
    40681 gcaggacgca gcaaaaaata cactcttggc gcggtaaata gcgtgattca gcgcgcttct
    40741 tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc
    40801 gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca agcgatgtgt
    40861 gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc ggttgctaat
    40921 tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac cgcctatgaa
    40981 ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac atcgtggttt
    41041 gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa aatcgtgttt
    41101 ctggaatcgc cgggttccat cactatggaa gtccacgacg ttccggcgat tgttgccgcc
    41161 gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc agccggtgtg
    41221 ctgtttaagg cgctggattt tggcatcgat gtttctattc aggccgccac caaatatctg
    41281 gttgggcatt cagatgcgat gattggcact gccgtgtgca atgcccgttg ctgggagcag
    41341 ctacgggaaa atgcctatct gatgggccag atggtcgatg ccgataccgc ctatataacc
    41401 agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa
    41461 gtggccgaat ggctggcaga acatccgcaa gttgcgcgag ttaaccaccc tgccctgcct
    41521 ggcagtaaag gacacgaatt ctggaaacga gactttacag gcagcagcgg gctattttcc
    41581 tttgtgctta agaaaaaact caatgatgaa gagctggcga actatctgga taacttcagt
    41641 ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc aaatcaacca
    41701 gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac cttgattcgc
    41761 ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc cggttttgca
    41821 cggattgtat aacattgcca cttttggaca attttgcaga cattttattg tgaaaagtct
    41881 taaattgttg cgtccgggat caaggcgtcc cggacgattc aggagtacaa taggcagata
    41941 aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata tcatcgctgc
    42001 gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg ttgtttactt
    42061 tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat ttttgccagg
    42121 cgacagcttg ttgatactgg caggcgcatt gattgcccaa ggggttatgg attttctgcc
    42181 tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt atattcaggg
    42241 gagctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc ctgctaaata
    42301 tcaccagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc tggctggacg
    42361 ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa
    42421 ccgccgcttc cagtttttca actggttaag tggattgctg tgggtcagcg tggtaaccag
    42481 ttttggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc aggtaatgac
    42541 gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt
    42601 ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc
    42661 gggaacgtat ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca
    42721 caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag ccgacgcgca
    42781 tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc ttatgcagac
    42841 ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa ttatggtgga
    42901 acgccgatac gctcatgttg gcttctgccg ccagttgctc gacgctcagg ttttcggtgt
    42961 atttattctc aattcgtttc agcacgcggc taatcagact gaagtgagtc tggcgactga
    43021 ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga
    43081 tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct
    43141 ccgccgcgca taaaatctct tctgataacg tggcggagtt aatcccgctg gctgccatcg
    43201 acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat
    43261 cgacattgag acgcaaccct gccagcggca cctctgacgt ggcataggtt tcgcactcaa
    43321 acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac acgcgttcat
    43381 tgatataacc gattttatgc ccggaaaaga gaattatgat gccaggctcg tacatcaccg
    43441 gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac atcgggcaac agttctgaca
    43501 aactattttc tttatttttc agtttattaa ctttatccgc cagcaagcga caaatctctt
    43561 cacgtttcat atcgcgtaat ttcttaggaa taatgcggca atttgattgt gcgcaatttt
    43621 gtagcatttc tccagcactc tggagaaata ggcaagacat tggcagaaat gagcattgag
    43681 agccagggcg ctggcgatca caatgaaaaa catcaggcag atcgttctct gccctcatat
    43741 tggcccagca aagggagcaa gtaatgaaca actttaatct gcacacccca acccgcattc
    43801 tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat tcctcacgat gctcgcgtat
    43861 tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg
    43921 ccctgaaagg catggacgtg ctggaatttg gcggtattga gccaaacccg gcttatgaaa
    43981 cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt gactttcctg ctggcggttg
    44041 gcggcggttc tgtactggac ggcaccaaat ttatcgccgc agcggctaac tatccggaaa
    44101 atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga
    44161 tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc caacgcaggc gcggtgatct
    44221 cccgtaaaac cacaggcgac aagcaggcgt tccattctgc acatgttcag ccggtatttg
    44281 ccgtgctcga tccggtttat acctacaccc tgccgccgcg tcaggtggct aacggcgtag
    44341 tggacgcctt tgtacacacc gtggaacagt atgttaccaa accggttgat gccaaaattc
    44401 aggaccgttt cgcagaaggc attttgctga cgctaatcga agatggtccg aaagccctga
    44461 aagagccaga aaactacgat gtgcgcgcca acgtcatgtg ggcggcgact caggcgctga
    44521 acggtttgat tggcactggc gtaccgcagg actgggcaac gcatatgctg ggccacgaac
    44581 tgactgcgat gcacggtctg gatcacgcgc aaacactggc tatcgtcctg cctgcactgt
    44641 ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca atatgctgaa cgcgtctgga
    44701 acatcactga aggttccgac gatgagcgta ttgacgccgc gattgccgca acccgcaatt
    44761 tctttgagca attaggcgtg ccgacccacc tctccgacta cggtctggac ggcagctcca
    44821 tcccggcttt gctgaaaaaa ctggaagagc acggcatgac ccaactgggc gaaaatcatg
    44881 acattacgtt ggatgtcagc cgccgtatat acgaagccgc ccgctaagct ttttacgcct
    44941 caaactttcg ttttcgggca tttcgtccag acttaagttc acaacacctc accggagcct
    45001 gctccggtga gttcatataa aggaggaacg tatggctaat ccaaccgtta ttaagctaca
    45061 ggatggcaat gtcatgcccc agctgggact gggcgtctgg caagcaagta atgaggaagt
    45121 aatcaccgcc attcaaaaag cgttagaagt gggttatcgc tcgattgata ccgccgcggc
    45181 ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca acagagaaga
    45241 actgttcatc accactaagc tgtggaacga cgaccacaag cgcccccgcg aagccctgct
    45301 cgacagcctg aaaaaactcc agcttgatta tatcgacctc tacttaatgc actggcccgt
    45361 tcccgctatc gaccattatg tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg
    45421 attaatcaaa agcatcggcg tgtgcaactt ccagatccat cacctgcaac gcctgattga
    45481 tgaaactggc gtgacgcctg tgataaacca gatcgaactt catccgctga tgcaacaacg
    45541 ccagctacac gcctggaacg cgacacacaa aatccagacc gaatcctgga gcccattagc
    45601 gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc gatctggcag ataaatacgg
    45661 caaaaccccg gcgcagattg ttatccgctg gcatctggat agcggcctgg tggtgatccc
    45721 gaaatcggtc acaccttcac gtattgccga aaactttgat gtctgggatt tccgtctcga
    45781 caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc
    45841 tgaccagttc ggcggctaac gtgcaaattc tcccggtggc ggtaatgttc cgctaccgga
    45901 cttttcagaa atcatttatt cccctcgcgt cccgcccgtt gttactcttc cttgttcagg
    45961 aatgccaaat ataaggacat catcatgcag agccggaagc tcttaaaaga acaactcatc
    46021 tatatccggg ataaacgcaa cggagaggtg aaaaacagat gaaaataata cttctgtttt
    46081 tagcagccct ggcaagtttt accgtacacg cgcagcctcc ttcacagacc gtagaacaaa
    46141 cagtccggca gatttatcag aactataaat cagatgccag tacgccttat ttcggtgaaa
    46201 ccggagagcg ggcgataact tcagcgcgta ttcaacaggc gcttaccctg aacgacaatc
    46261 ttacactacc gggcaatatt ggctggctgg attatgatcc ggtttgtgat tgtcaggatt
    46321 ttggcgatct ggtgttagaa agcgtagcga taacccaaac tgacgtcgat catgccgatg
    46381 ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa gaccacacag acactgaaaa
    46441 tggtggcgga aaatggtcgt tgggtcattg atgatattgt cagcaatcat ggcagcgtct
    46501 tacaagcagt taatagcgag aatgaaaaaa cgctggccgc tttagcttca ttgcagaaag
    46561 aacagccaga atcctttgtt gctgaactct ttgaacatat tgctgattat agctggccgt
    46621 ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa tgccttctat aaaaccacct
    46681 tcaagacggc caataatccc gatgaagata tgcaaataga acggcaattt atttacgaca
    46741 atccgatctg ttttggcgaa gagtcgctat tttcacgcgt tgatgaaatt cgagtcctgg
    46801 agaaaaccgc cgattccgcc cgcattcatg ttcgttttac gctgaccaat ggcaacaatg
    46861 aagagcaaga gctggtttta cagcggcgcg aaggtaagtg ggaaatcgct gattttatcc
    46921 gcccgaacag cggcagccta cttaagcaga ttgaggcaaa aaccgccgcc agattaaagc
    46981 aatgagctga attaaataac aattagccgg aacaataaat aaaagggaac actatatgaa
    47041 aacgattttc accgtgggag ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa
    47101 tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca ttaaatgcca aaatcgcccg
    47161 gcttgagcag gatatgaaag cactacgccc acaaatctat gctgccaaat ccgaagctaa
    47221 cagagccaat acgcgtcttg atgcacagga ctattttgat tgcctgcgct gcttgcgtat
    47281 gtacgcagaa tgataaaaaa atccccggca gcatgtcagt tgccggggat tttttttaac
    47341 gtccaaccgc cgctttaggg cgtttcttcg caccagcatt caccggacga gattgcgtag
    47401 acgacgcttt ttttgccgta gcaggcgtct gacgctgagt cgccatcggc gtatgtttag
    47461 tcaacgccgg acgggtattg cggttctggc gacgagcttc acgcatctct tcaatggttg
    47521 gcgcaggcac taagcaatcg cgacggctgc caatcagatg ctttttgccc atcgcttcca
    47581 gcgcctgacg gattaacggc cagtttgccg gatcgtggta acgcaacaac gctttatgca
    47641 aacgacgctg tttgtcgccc ttcggtacga agacgccttc gctcttataa ccaatcttcg
    47701 ccagcggttt tttcccggtg taatacatgg tggttgagtt cgccagcggc gatgggtaga
    47761 agttctgtac ctggtcgaga cggaaacgat gctttttcag ccacagcgcc aaattcacca
    47821 tatcttcatc acgcgtaccg gggtgcgcag agatgaaata cgggatcaga tactgctctt
    47881 tacctgcctg tttcgagtag gtatcgaaca gctctttaaa gcggtcatag ctgcccatgc
    47941 ccggcttcat catcttcgac aacggccctt cttcggtatg ttccggagca atcttcagat
    48001 aaccgccgac gtgatgggtc gccaactctt tgatatagcg cggatcttcc acggcgatgt
    48061 cataacgtac gcctgaggcg ataaggattt ttttaatgcc tttcagatca cgcgcacggc
    48121 gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg cggacaaata tccggataaa
    48181 cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga tttgcagcgc aacatataca
    48241 tgttggcagt tggcccaccg agatcggaaa tcacgcccgt aaaacctgga acggtgtcgc
    48301 ggatcgcttc gatctcatta atgatcgaat cttccgaacg gctctgaata atgcgccctt
    48361 cgtgctcggt aatagaacag aaagaacagc cgccaaagca gccgcgcata atgttgaccg
    48421 aaaaacggat catttcgtaa gccggaatac gggcattgcc ataggccgga tgtggcacgc
    48481 gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt agaaagcggg atagcaggcg
    48541 ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa tgcgcgggcg cagcctgggt
    48601 tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag cactttatcg cccttcactt
    48661 tctcgaaaga aggcagcaat acgtaggttt tttcccacgg tttctggcgc ggtggctgca
    48721 cgattacagc tttcgcttcc tgctttttcg gcgcaacagg tttgttatcc gcacacggca
    48781 aatcttcgcc atacggatgc gggattgggg cgattttccc tggggtatca agacgggtgg
    48841 aatccacacc gctccagcca ggcagcgcct cttttacgat aattgcggta ttacgcacat
    48901 cgcggatttc actaatcggc tcgcccatcg ccagacgatg cactacttcc accagcggac
    48961 gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc taccagaacg gaacggcgca
    49021 cggtatcgga ccagtaatca taatgcgcgg tacggcgcag gctagcctca ataccgccga
    49081 ggatcaccgg cacatctttc cacgcctctt tacaacgctg ggtataaacc agcgtggcgc
    49141 gatccggtcg cttacccgcg acattatctg gcgtgtaggc atcatcatga cgtaaacggc
    49201 gatcggcggt atagcggttg atcatcgaat ccatgttgcc agcagtaaca ccaaaaaaca
    49261 gattcggttt acccagacgc ataaagtcgt ctttgctgct ccagtccggc tgggcgatga
    49321 tcccgacgcg aaagccctgc gcttccagca tacgaccgca aatcgccatc ccgaagcttg
    49381 ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc gcagctatcc cagccaagtt
    49441 gatccatctc ttcacgagac atcggcaaaa acggtgccgg tccaaaacag gcggcccagt
    49501 actgcggcca ggagaacagg tcgcgatccg gttggatcag ggagatagag ctcattttgc
    49561 ttccagaaat gataaaaaaa taatcaaagg ccggggatta taagccggaa cgaaagagaa
    49621 atcgaaaggt attccatact cgccctcctc gggcgagtat gaagattacg gtaccggatt
    49681 gaccaacagt tgcccaatcg agccacggtc cgccatttcc agcgtctgac tgttgaagta
    49741 gaacgggaag tgcgcccagg aaggctgacc gaaataaaca agcagctcca cttgtccgtc
    49801 aacccaaacg gtatctttcc agcctctgtc ttccgggaac ggcatcgcgc cattcacgtt
    49861 acggatctgg aacattacgc cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt
    49921 ccagcgttcc cacgttcctt gctgcgcggt gacatcaata cggttgacgt cccacagctg
    49981 tccattaata cccgggtcat cacccagact gatatcgcga ctgcgaattg gcgaaccagc
    50041 catgatttca gttggcagca agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc
    50101 ggttgggcgc agcgttagca ccaaggtaga aaccagaata ctggatggct caaagaagcc
    50161 gcgaatacga tcaacaatgc tcgccgcttc accacaggtg atcgacactt catcgccgtt
    50221 gctcatatcc accagaatct cgcggcgctc gcccggtgcc agcgaaagtt gcttcaccga
    50281 tacaggagca ggcaggaatc cctgatcgcc agaaatcaca tgtaacgggc gaccatcgct
    50341 catctgtagt tgatagcgac gagagttcga cgcgttcagc agtcgcaagc gcacccagcc
    50401 acgcgagact tcaacgtacg ggctttgtac accgttaacc agcagcgtat caccaacaaa
    50461 gccgccgctt cccggttcgt tgtattctgg cgtaccaaag ttatccagcc gtttatcctg
    50521 gataatgacc ggaaaatcat ccacaccata atggttgggg ataggcagcg acttgctgac
    50581 ttcatcttcc accagccaca ttccggcaag gccgttatag acctgctgag ccgtgcggtt
    50641 gggagtattg gcgtgatacc acagagtagc tgcgttctgg cgaataggca gtacgggtgc
    50701 ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc atcagcgggc ctggtacctg
    50761 tagcccggcc accgtcattg agacattttc tgtcaggcgg ttgctgtaaa taagcttaac
    50821 atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa cgaccattga ttccccagac
    50881 cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt tgtacagtca taaacagcgg
    50941 ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc ggttgctgtt gcccggctgc
    51001 gctggccttc aggggaacag cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg
    51061 acgccgactg agtgacatat ttgctccatg taaaactggc taaagtaata ccgtgcagga
    51121 aagtgttccc cgcaaaaata cagattgccg acttaaactt ttccggcggc ttcgcgttct
    51181 gcgacttctt tatcgagctc ggcgattttt tgttccatta tcgaacgaca atgggcagcc
    51241 agctcacgaa cctgatcttt gccatactga ctgacgtcaa ttggcggcag catttcgaca
    51301 atcaccagac cgttgtgcag tcgattaaga ttaatcttat tcgaagttgt agagacgcac
    51361 acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa atgctccagt cttgaacggt
    51421 agcaggccgc gaccacggct gcgggttcct tccgggaaca tccagatgga aatacggcgt
    51481 tttttgaaat gattcacgac ttccgcaatg gtgccgtgag ctttagtgcg attgtttctg
    51541 tcgatcaata agttgccggt taaccagtac aactgcccga agaaggggat ccacagcaag
    51601 ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt tcgatgctgt caccatgtca
    51661 tagttgttct ggtggttagc gatatagata gcattgccgt agttttcagc atctgcaggt
    51721 ttgcggcact caactttcag gccgaacagt ggcgcaagac ggccaaacat atgcccaaag
    51781 gtggccacat gtttcgggtt acgcgggctg aaaaggcagt aaatggagcc gaatacacag
    51841 actaagatgc tgtaaatcac ggtaataata agacgaaaga tatatagcat aacaccctct
    51901 gaaggtctga gagactggca ttgtacgtca cctgaaatca ggttagggca ttgttaaaag
    51961 ccgctctctg gaaaagcgta ttgttaatcg caacgcatga ataaacaacg gtttcaaggg
    52021 aatttttatc gtttccgggg gttgaatacg ctgccggatg acgacttaac gtttcatccg
    52081 gcgttccttg ctagcgggag gaaacagcgc cctccccggc atattactct tcgctatcac
    52141 cgctgctggc acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca
    52201 tcaacgtacc gcggcgtcca cgttcgccag tgactttctg tagctcttcc ggacgcagtt
    52261 taattttgcg tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacagtt
    52321 gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc
    52381 ctttgccctt cgacagctgc ggcagatcac ttaccgggaa catcaacata cggcctgcct
    52441 gagtgattgc cagcagcata tcggaagcat cttcaatcac caccggcggc ataacatggg
    52501 cattttccgg taaggtgatc aaagccttac ccgcacggtt acgcgctacc agatcgttaa
    52561 aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt
    52621 ccatcagcat atggtcaacg gtcgccccag gcggcaacgt taatttgccg gtgagtggtt
    52681 cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat ggcatagcta cgaccggtgg
    52741 aatcaacaaa cactaccggt tggttactct tacctttcac cgccgctttg aagctatcac
    52801 ccgctttata attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc
    52861 ccatctgcga cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg
    52921 ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt
    52981 ctgcctgcag ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct
    53041 gcaactggtc gcgctctttt tccagctcac tctgctcacc gcgaatcttc atctcttcca
    53101 gtttggcaag atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc
    53161 caaaccgcga catcagcgcc ggtttcggtt catcttcgtt acgaatgatc tcaatcactt
    53221 cgtcgatgtt gagaaacgcc accagcaaac cttccaggat atgcaggcgc ttgaggactt
    53281 tctccagacg atagttcagt cggcggcgca cggtatcgcg acggaacacc agccattcgg
    53341 agaggatttc cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattgaggt
    53401 taatgcgata gctcttttcc agatcggtgg tagcgaagag gtggttcatc acctgatcca
    53461 tatccacgcg gttggaacgc ggcacaatca ccagacgggt cgggttctcg tggtcagatt
    53521 catcgcgcag atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct
    53581 ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct gataaccacc gcgccatctt
    53641 ctttcttcca caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgaa
    53701 tctcggcgcg cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat
    53761 ccagcagctg atcgagcgta gttttcggct ggtcgattaa tgcgattgcc gcctgagcta
    53821 cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc
    53881 cgttaagcaa aatgtttggc agacgggcag gtagcatttt cggctcctgc aaagtgccgt
    53941 cgaagtttgg cacccagtca gccgtcccct gccccagctc gctcaatagc agctcggaat
    54001 atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg
    54061 cgccccagtt cccctgacca tcaaccagcg gataacggta agagaacggc tgcgccatca
    54121 ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac
    54181 cgacggtacg ggccgatttt ttaaatttgg cgctggcatt caggcccagt tcagacatcg
    54241 catacacaat gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt
    54301 ccatgatcac gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa
    54361 ggcgctctgc catatcgctc attaattctg attcctcaac ttattcgcca gccttcaaag
    54421 gggcaatatt gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg
    54481 aatagcgccg ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa
    54541 ctctttgctg tagacttgat cggcatagag ggcagcacaa ggttgcgccc atgccacacc
    54601 actcggcatg ggggtccgct ccgggcgatt cgagaagttg aggttatgcg agtccaggga
    54661 agcctaaagc atcaaagtag tcgactgtaa gtatacggtt cagcccaatt tagctgtgtc
    54721 tccaccagtg ccccgcgcct cctgccataa gtgcggcttg ctcatggata tccccgagac
    54781 ggcttacggc gcaccggata ataaatttcg cgtatacatg gtattcctta ccggagaaat
    54841 tataagccca acaacaatac gcctgtgaca ttgttcgctc aaacttatcg cgagtgattt
    54901 ggctcacact ccggtaaaga ttccccatca cgccaacggc acataaacat ccgtttccag
    54961 tcgctgctct gtcacccctt cggcaagatt ggtgtagtga aacagaatcg gtgctttacg
    55021 cattttctcg ccgcttgcag gcagccagtg gcgaataatg ccccatatcg tgtgtgaaat
    55081 atcgtccagc tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa gttcaccatt
    55141 gctcacacca taacgattat cgggaatcgg ttcgctaacg ctgccgcaga tatcaaagcg
    55201 aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg acgccaaaag tctggctttg
    55261 gttaacagga gaaagccccg tctctttacg ccacatgata aatttcgcca cactgtagtt
    55321 gagcaactca gggctacagc ggtgcggcaa catcgccaca gggattgatg ggaaatcgat
    55381 aatgtttaca tccagtgtca ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa
    55441 cgcgtcccgg gagcggtaac aattacggat ttactttgcg gatctgtttg acgtcaattt
    55501 caacagaatt ccagtcttta tcgacttcac cctgaatctc aaccgtatct ttcggcgtca
    55561 ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt gatagtaccg ctggcatctt
    55621 taaacacgta gagatcgtca gagatgcgtt caacgatatt gccgcgcagg gttacccagg
    55681 tgtcgtcacg cagggatttt gcgctttcta cagtcgttac gctgccgttc ggcccctgaa
    55741 atcctccggc ctgactttgc gttaccgatg ggccagaaaa accgccctgc tctgctgcca
    55801 tcaccggtgc actgcacagg gccattactg cgattactgc tgcgaatttt ttcatgttta
    55861 ttactccctt taatgtctgt ttccgagcat ttaacaagat agtccttaac aacttcttaa
    55921 gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac aacacggttt actggcagca
    55981 aatacggtta tcgcagggat gaaaaaatgc gaattttact gatagaagat gacatgctga
    56041 ttggtgacgg catcaaaact ggccttagta aaatgggttt tagtgtcgac tggtttacac
    56101 aaggtcgtca gggaaaagag gcgctttata gcgcacctta tgatgcggtg atcctggatt
    56161 taaccttacc aggcatggat ggtcgcgata ttttgcgcga atggcgagag aaaggtcagc
    56221 gtgagccggt actgaacctg accgcgcgtg atgcgttagc ggaacgtgta gaagggctgc
    56281 gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc gccgccaagc
    56341 tggaggctct gatgcgccga accaacggcc aggccagcaa cgagctgcgc cacggtaacg
    56401 tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg cgaaccctta acactgaaac
    56461 caaaagaatt tgccctgctg gaattactga tgcgtaacgc tggtcgggta ctgccgcgca
    56521 aactgattga agagaaactg tatacctggg acgaagaggt caccagtaat gccgttgaag
    56581 tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt tattcgtacc gtgcatggta
    56641 ttggctacac attaggtgag aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct
    56701 gacgctaatc tttttaattc tggcctcggt gacctggctg ctttccagct ttgtcgcctg
    56761 gaaacaaaca acggataacg tcgatgaatt gttcgacacc caactgatgc tgtttgccaa
    56821 acggttaagt acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc
    56881 aaataaatta aaacacggtc atgttgatga cgatgcgctg acctttgcca tctttaccca
    56941 cgacggcaga atggtcctta atgatggcga taacggagaa gatattccct atagctatca
    57001 acgggaaggt tttgctgacg ggcaactggt cggtgaagac gatccttggc gttttgtctg
    57061 gatgacctca cctgatggca aatatcgcat cgttgttggc caggaatggg aataccgtga
    57121 agacatggcg ctggcgattg ttgccgggca attgatcccg tggctggtcg cactgccgat
    57181 tatgttaatc atcatgatgg tactactggg tcgtgaactc gcgccgctga acaaactggc
    57241 gctggcacta cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga ctggcgtacc
    57301 cagcgaagtg cggccactgg ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat
    57361 gatggttcga gaacgacgct ttacctccga tgcagctcac gaactacgta gcccgttaac
    57421 agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac gatgatccgc aggcgcggaa
    57481 aaaagcactg ctccaattac attccgggat cgatcgcgct actcgtctgg ttgatcaact
    57541 gctcacgcta tcgcggctgg actcactgga taaccttcag gacgtcgcgg agatcccgct
    57601 tgaagatctc ctacaatcgt cggtgatgga tatttaccac acggcgcagc aggcgaaaat
    57661 tgacgtgcga ctgacactca atgcccacgg catcaaacgc accggacaac cgctattgct
    57721 aagtttgttg gtgcgaaatt tgctggataa cggccccggt gtgacaccag aggcactggc
    57781 gcgaattggc gaacgcttct atcgcccacc cggacaaacc gctaccggca gtggacttgg
    57841 gctatcgatt gtccagcgaa tcgccaagct gcatgacatg aatgttgagt ttgggaatgc
    57901 ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt ctctgtctat gagagccgtt
    57961 aaaacgactc tcatagattt tactaatagc aaaatataaa ccgtccccaa aaaagccacc
    58021 aaccacaaac cagacaaata acgagcgaga atacatatag ccaaaactcg aacatttccc
    58081 gcataaagag ttttcttaag ataagaataa taagtggcgt aagaagaaaa aatgctgcaa
    58141 tcattgcttg caatatacga tcttcgcctg aaacggaagg tatactgcaa atattgcctc
    58201 ccgactcaag catccagtca tacttattga aagcaaaaaa agaatctatt aaccaataag
    58261 taagcattat atatagcaat gcttcaaata ttgttttaat tttctgcttt aacatatatc
    58321 agtacgctca taaaaatcca tttataatta tcaacatgac acgttgatat tttcatcttt
    58381 tacttattca aagccatatt ttcaatcccc aaaaaaaccg aaccaaacgt gtaaattatt
    58441 ccggtgcgag taagaattat gaggaatggc tatcagtatt gtcattttca gaaaatattt
    58501 atcctgcatc ggtgagtcag agctagatca gacttttgct aaattcgcaa aagactttgc
    58561 acattttgct aatttcacag taccgctctg tgacgtacta tagtcgacaa acgtctcacc
    58621 ttgagattaa aaaatgagca acatcctgat tatcaacggc gcgaaaaaat tcgcccactc
    58681 caatggtcaa ctgaacgaca ccctgaccga agtcgcggat ggcacactgc gcgacctcgg
    58741 gcatgatgtc cgcatcgttc gcgccgacag cgactacaat gtcaaagcgg aagtacaaaa
    58801 ctttctctgg gctgatgtgg tgatctggca gatgccaggc tggtggatgg gcgcgccgtg
    58861 gacagtgaaa aaatatattg atgatgtatt caccgaaggt cacgggactc tgtatgccag
    58921 cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt tccggcagcc tggtacaggg
    58981 caaaaaatat atgctttctc taacctggaa cgcaccaatg gaagccttca ccgaaaaaga
    59041 tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg ccgttccata aagcaaacca
    59101 attcctcggt atggaaccgc tgccgacatt tatcgctaat gacgtgataa aaatgcctga
    59161 tgttccccgc tatactgaag aatatcgcaa gcatcttgtg gaaatttttg gttaactaga
    59221 gctcaggctt tagaaggagt taaccatgct taccgtaatc gcagaaatcc gtactcgtcc
    59281 tggtcaacat caccgtcagg cggtattgga tcagtttgct aaaatcgttc caaccgtact
    59341 gaaagaagaa ggttgccacg gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt
    59401 ccagtctatg gcaccggatt ctatcgtgat gattgagcag tgggaaagca tcgcgcacct
    59461 tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt
    59521 gctggagatg aatatccgta ttctgcagcc agggatttaa tcctgccttg tttgcccggc
    59581 catcctgacc gggcaatgtt ctttccttta aacctcaatc tccgccatgt cgcctttctc
    59641 ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc agcagcatat ccatcatcgc
    59701 gtcagtacgc tgatcgtctt catcatcgat agtcaactgc accagacggc gagtgttcgg
    59761 atcaagcgtg gtttcacgca attgcatcgg gttcatttcc cccagacctt taaaacgctg
    59821 gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt acgccctctt tctcttcttc
    59881 cgtcagcgcg taataaacct ctttcccgag atcaatacgg tagagcggtg gcagtgcgac
    59941 gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt acgaacaaag cgcagagcag
    60001 cgtggcaatg tgcagaccat cagagtccgc atccgcgagg atacagattt tgccataacg
    60061 aagctggctc agatcgtcgc tgtcaggatc gataccgatc gctaccgaaa tatcatgcac
    60121 ttcctgcgag gccagcactt cgtcggaaga aacttcccag gtgttaagga tcttaccttt
    60181 cagcggcatg atcgcctgat attcacgatc gcgcgcctgc ttggcagatc cgcctgcgga
    60241 gtcaccttcc actaggaaca gttcggtacg gttaaggccc tgcgcggtac aatcagccag
    60301 tttgccaggc agcgccgggc cgctggtcag ctttttacgc accacttttt tagccgcacg
    60361 catacgacgc tgggcgctgg aaatcgccat ctctgccagc aactccgccg cctgaacgtt
    60421 ctggttcagc cacaggatga aggcatcttt caccacgccc gaaacgaatg ccgcacactg
    60481 acgcgaagag agacgctctt tggtctgccc ggcaaactgc ggatcctgca ttttaaccga
    60541 cagaacatag gcgcagcgat cccagatatc ttccgccgac agcttcacgc cgcgcggcag
    60601 aatgttgcgg tattcacaga actcacgcat cgcgtccaac aggccctgac gcagaccatt
    60661 aacatgggta ccgccctgca tcgttgggat aaggttgacg tagctttcgg tcagcagttc
    60721 accgccttcc ggcagccaca gtagcgccca gtccacagct tcagtatcgc cagcgaaatt
    60781 accgataaac ggtttttctg gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc
    60841 attcagaccg tcctgatagc accagcgctg ttcggtgttg ttgatctcat ctttaaaggt
    60901 aatctcaacg ccggggcaca gtactgcttt ggccttcagc acatgcgtca ggcgtgaaac
    60961 agaaaaacgc gggctgtcaa agaaggtttc atccggccag aagtgcacgc tggtaccggt
    61021 attgcgttta ccgcaagtgc cgacaacctg taaatcctgc accttttcgc cattttcaaa
    61081 ggcgatgtta taaacctgac catcgcggcg cacgttaact tctacgcgct tcgacaaggc
    61141 gttaactacc gaaatcccca cgccatgcag gccgccagag aactggtaat ttttgttaga
    61201 gaatttaccg cctgcatgca gacggcaaag aatcagttca accgccggta ctccttcttc
    61261 cgggtgaata tccaccggca tcccgcgccc atcgtcaatc acttctaacg actggtcagc
    61321 atgtaagatt acatccacgc gttttgcgtg acccgccagt gcttcatcga cactgttatc
    61381 aataacttct tgccccaaat ggttagggcg agtggtatcg gtatacatcc ccggacggcg
    61441 gcgaaccggc tcaagcccgg tgagtacctc aatggcatca gcgttataag tttgcgtcat
    61501 ggtttaagtt agcaattcga gttgatcgtc agagatggtg cagaccaaga aaatcgacga
    61561 tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt gccgccttct atgacagtct
    61621 ggcggcagga agcgtagtac gccaccgcct ggcggtaatc cagcacttca tctcccgtct
    61681 gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg actttgagat
    61741 cgtaaatatg gcgtgactct agcacatatt gctgcccggt gtaggggttc tcgttctgac
    61801 cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg gtttaccacc actgcgggca
    61861 gcataaaaca ttgcgacaac caggtggcgt aatatccccc cagtgacgaa ccgacaatac
    61921 ccagcgaatc gccgccatgt tccaggacaa tggattccag cagttctgcc gcgtcggaag
    61981 gatacggcgg caactgcgga atgatcatct caacgtcagg gtgatgttcc gccagccagt
    62041 tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa
    62101 gcgtagacat cagtagcctt ctgaagcggt atcaggttgg aaacgtgtgt ccgccaggcg
    62161 atgcacctcg gtggtcagcg tgccatcagc atgtaactcg agagtacgcc agccgggcgc
    62221 gatagtatcc agcgtaaagt tggaacagtg cggcttaaac tgcacacagg tcgacggcgt
    62281 tgccagcagg cggcgaccgt tccagtcgag atccagctcc tgatgaatat gaccgcacag
    62341 caagtatttg acgtgcggaa acttcgccag cacggtatcc agttcgcccg cgttacgcag
    62401 actgtgttga tcgagccaac tacaacccgc aggtagcgga tgatgatgca gcagcagcaa
    62461 cgtatggcgt tctggcgcat cggccagttt acgttccagc cactcaagct gaaactcgct
    62521 cagctcaccg tgcggcacgc caaacacctg gctatccagc aacaggattt gccattgctc
    62581 accaataaac acccgcttcg ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc
    62641 gggctggaaa tcgtggttgc ccggcagcca gacgcagggc gcacgaaaac ttgcgatgcc
    62701 ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac
    62761 aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc agcaccgcct ggtagctttc
    62821 ccaggtgttt acccctaaca gggcttcgtg cttttgtgca aacaggtgag tatcggtaat
    62881 ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg gttaacaggc tttccaaatg
    62941 gtgtccttag gtttcacgac gctagtaaac cggaatcgcc atcgctccat gtgctaaaca
    63001 gtatcgcaac cagtccgcta aaaactggtt aatttgatgc ttttcgtcgc gttgatgcaa
    63061 ctttttatta ggataatcat accgcgcttt gaagcgaaaa atctgctggc ttgaacacac
    63121 ttcagccacc atcgcgtcat gatacagacg caccgtcatt gacggaaggc tccagtaact
    63181 gatcgcgggc gcagtctgtt ctattgtcac tagggtagtg tatcgggtcg attccacaat
    63241 cgtcagccga tattgtgcgt ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt
    63301 gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg cacaggcgca tcatttcagg
    63361 aaagtcaggt gtgtaacgct tcatttatgc ccactcattt tttaacgctt gatgatgcag
    63421 ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct cttctaccca
    63481 ctggtatgcc tgttcccggc ttaccacatg aacgcgaata tcttcgtttt catcagccag
    63541 accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc accataattg acgaacgctc
    63601 actggtgccc cccgggcttg ccaggaaact taacaccggt ttggtccgtt tgactatcag
    63661 tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc acactttcac cctcttcaat
    63721 catcccggca accatctcca gtagccaggg ggtttcgctg gtgtcgtacg cggcaatccg
    63781 aatctgctca atcagcacaa cttcatcacg cactgggtca aagggtagca agactgcggc
    63841 gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc atttgcccgt tgaatagacg
    63901 atgacgaaat ctataaagat ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat
    63961 aatttctaca tcgtttttgc caaatgtaac gggcaggttg tctggcttaa gcattgttaa
    64021 tgtcctagca ctaatagtga attaaatgtg aatttcagcg acgtttgact gccgtttgag
    64081 cagtcatatg ttaaattgag gcacattaac gccctatggc acgtaacgcc aaccttttgc
    64141 ggtagcggct tctgctagaa tccgcaataa ttttacagtt tgatcgcgct aaatactgct
    64201 tcaccacaag gaatgcaaat gaagaaattg ctccccattc ttatcggcct gagcctttct
    64261 gggttcagtt cgttgagcca ggccgagaac ctgatgcaag tttatcagca agcacgcctt
    64321 agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat
    64381 gaagcgcgca gtccattact gccacagcta ggtttaggtg cagattacac ctatagcaac
    64441 ggctaccgcg acgcgaacgg catcaactcg aacgcgacca gtgcgtccct gcagttaact
    64501 caatccattt ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa agcagcaggg
    64561 attcaggacg tcacatatca gaccgatcag caaaccttga tcctcaacac cgcgaccgct
    64621 tatttcaacg tgttgaatgc tattgacgtt ctttcctata cacaggcaca aaaagaagcg
    64681 atctaccgtc aattagatca aaccacccaa cgttttaacg tgggcctggt agcgatcacc
    64741 gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg cgaacgaagt gaccgcacgt
    64801 aataaccttg ataacgcggt agagcagctg cgccagatca ccggtaacta ctatccggaa
    64861 ctggcggcgc tgaatgtcga aaactttaaa accgacaaac cacagccggt taacgcgctg
    64921 ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg cacgcttgag ccaggacctg
    64981 gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac cgactctgga tttaacggct
    65041 tctaccggga tttctgacac ctcttatagc ggttcgaaaa cccgtggtgc cgctggtacc
    65101 cagtatgacg atagcaatat gggccagaac aaagttggcc tgagcttctc gctgccgatt
    65161 tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac agtacaactt tgttggtgcc
    65221 agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac
    65281 aacattaatg cttctatcag tagtattaac gcctacaaac aagccgtagt ttccgcgcaa
    65341 agctcattag acgcgatgga agcgggctac tcggtcggta cgcgtaccat tgttgatgtg
    65401 ttggatgcga ccaccacgct gtacaacgcc aagcaagagc tggcgaatgc gcgttataac
    65461 tacctgatta atcagctgaa tattaagtca gccctgggta cgttgaacga gcaggatttg
    65521 ctggcactga acaatgcgct gagcaaaccg gtttccacta atccggaaaa cgttgccccg
    65581 caaacgccgg aacagaatgc tattgctgat ggttatgcgc ctgatagccc ggcacccgtc
    65641 gttcagcaaa catccgcacg cactaccacc agtaacggtc ataacccttt ccgtaactga
    65701 tgacgacgac ggggcttcgg ccccgtctga acgtaaggca acgtaaagat acgggttatc
    65761 tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc catcctctat tctgatgggt
    65821 atttaccact ggtcccggaa gacaaaaatg aaacggacaa aatccatacg ccacgcatcg
    65881 ttccgcaaaa actggagcgc acgccatctg acaccagtcg ctctcgcggt tgccactgtt
    65941 tttatgctgg ctggctgtga aaagagtgat gaaacagtgt ctctctatca aaatgctgac
    66001 gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg
    66061 ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa
    66121 tttggtgaag gtcagtgtca gcaagcacca gcccaggctg gcatggcacc agaaaaccag
    66181 gcgcaggctc agcaatccag cgggagtttc tggatgccgc tgatggccgg ttacatgatg
    66241 gggcgtctga tgggcggcgg cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac
    66301 ccggccagtc cggcttacgg taaatatacc gacgcgacgg gtaaaaacta tggcgcagcc
    66361 cagccaggcc gcaccatgac cgtaccgaag acggcaatgg caccaaaacc ggcgaccacc
    66421 actaccgtta cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt
    66481 agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat accgatggaa agagtcagta
    66541 ttaccgagcg cccggactgg cgtgagaaag cccacgaata cggtttcaat tttcacacca
    66601 tgtacggcga gccgtactgg tgtgaagatg cttactacaa gttgaccctc gcccaggttg
    66661 aaaagctgga agaagtcacc gccgaactgc accagatgtg cctgaaagtg gtggaaaaag
    66721 tgatcgccag cgatgagctg atgaccaaat tccgcatacc aaaacacacc tggagttttg
    66781 tgcgtcaatc atggctgact caccagccat cgctttattc gcgtcttgat ctggcgtggg
    66841 atggcactgg cgaacctaaa cttctggaaa ataacgccga tacgccaacg tcactatacg
    66901 aggcggcgtt ctttcagtgg atctggctgg aagatcagct taacgccggt aacttgtcgg
    66961 agggcagcga ccagtttaac agtctgcaag aaaaactgat cgatcgcttc gttgagctgc
    67021 gtgaacagta tggcttccag ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc
    67081 gcggaaccat tcagtatttg caggactgcg caaccgaagc tgaaatcgcc accgagttcc
    67141 tctacatcga cgagataggt ttaggcgaaa aaggccagtt cacggattta caggatcagg
    67201 tgatttctaa cctgttcaag ctttatccgt gggaatttat gctgcgcgaa atgttctcca
    67261 ccaagctgga agatgctggc gtacgctggc tggaaccggc gtggaagagc attatctcca
    67321 acaaggcact tctaccgcta ctgtgggaga tgttcccgaa tcacccgaac ctgctgcccg
    67381 cttattttgc ggaagatgat catccgcaaa tggaaaaata tgtggttaaa ccgatcttct
    67441 cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa aaccattgaa gcagcggaag
    67501 gtccgtatgg cgaagaaggg atgattgttc agcaattcca cccgttaccg aaattcggcg
    67561 acagctatat gctgattggt agctggctgg tgaacgatca acctgccgga attggcattc
    67621 gtgaagaccg tgcgttgatc acccaggata tgtctcggtt ttatccacat atttttgttg
    67681 aataagccac gataccggat ggcactcgcc atccggtaat tgttagccta tctgcaccga
    67741 cagcatactt aaactgccca tttctatacc atcgaccgga atggtaattg gctcctgccc
    67801 atcccacaca cctaacacat acaacaacgg cagatagtgc tctggcgttg ggttcgataa
    67861 cgcgccacct tcatggtcga gataattcac cagaggatgc tgctctaccg gcccttgcca
    67921 cgtcagattc gctttcacat actcattaaa cgacgtcgcc cacggatacg gtgaactatc
    67981 accgtgccac ttcactgtgc gcaggttatg caccacgtta ccgctggcga ccaacattat
    68041 tccttcatca cgcagcgccg cgagtttgcg ccccatttcg aaatgccagg cgacaggttt
    68101 gctactgtcg atactcaact gcaccatagg gatatcggcg tcaggataca tcttaatcag
    68161 cacgccccac gagccgtggt caaagcccca ggcttcttta tccagcgtca ccgggatcgg
    68221 cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa cccggagcag gataatgcgt
    68281 atcgtacagc gcctgcggga agccaccaaa gtcatgaatc gtgggcggcg tctccatcgc
    68341 ggtcactcct gttccacggg taaaccagtg agccgaaacc accacaatcg cttgcgggcg
    68401 tggcaatgtc atccccaact tctgccagct gcgggtatac aaattatctt ccagcacgtt
    68461 catcggacta ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca tgatgatatc
    68521 cttaactaaa ggtgtcattt tgatatcctc acaatacgct tgttcggcgg agtaagaacc
    68581 cggataacaa tgatgatgat catcagttat tttgacgatc tgcctgaagg tgaagattta
    68641 taaggagttg tcgatgtcag tacctctcat tctgaccata ctggcggggg cagccacgtt
    68701 tattggcgcg tttctcggcg ttctcggaca aaaaccctcg aaccgcttac tggcgttttc
    68761 gctaggtttt gcggcgggga tcatgttgct catctcatta atggaaatgc ttcctgccgc
    68821 actagcagct gaaggaatgt cgccggtgtt gggttatgga atgtttatct tcggtctgct
    68881 tggctacttt ggcctggacc gcatgttgcc gcatgcgcat ccgcaggatt taatgcaaaa
    68941 atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc attctgctca ctctcggcat
    69001 cagtctgcat aacttcccgg aagggattgc cacctttgtc acggcgagca gcaacctgga
    69061 gctgggattt ggcatcgcac tggccgtcgc gttgcacaat atccctgaag gtctggcagt
    69121 ggcaggcccg gtttatgcgg caacggggtc taaacgcacc gcgattctgt gggcggggat
    69181 ttctggacta gcagaaattc ttggtggtgt gctggcgtgg ttaatcctcg gtagcatgat
    69241 ttccccggtg gtcatggcgg caatcatggc ggcggttgca ggaattatgg tggcgctctc
    69301 ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct aataataacc ccagctatgg
    69361 cgtactatgt ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa cggcgggaat
    69421 tggttaaaaa caacatcaga agttgtccag cacatggaca acttctgcca gcttcacgaa
    69481 agccttattc taccttttcg gctgtctctt ctctcgtact gtttaagtat ttgttccgcg
    69541 cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca ataacgtttc aaatgcagga
    69601 aataacccac cacaaccaga gcaatcaata caatccagta ccaggcgata aacatccttt
    69661 tacccttaat atttaacaat actaaacatt tagcgcataa atttcacata tcctttttcg
    69721 gatatattca tatggtcgca taataacgga acaattatat gattttgatc ataatgaaaa
    69781 agaatgcctg atattaatga aattttctca tagaaagaga agcggaatta acatcttgca
    69841 tagcagggca aaataaaaac cgaagccagc tacaacttcg gtttcttgat taaggcagta
    69901 attaagcagc ggttttcagc tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc
    69961 ttcaatagtc acgagcgcca tattgtgttt attggcaaac tcaatgcact ctggtgcacg
    70021 cgccatcgtg ccatcgtcat tagtcagctc acacagcaca ccagccggtt taaagcctgc
    70081 cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca cgcgtcagta caccacctgc
    70141 ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc
    70201 ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca gaaacaccgg tagtcacacc
    70261 ttcagccgct tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt tattttctac
    70321 catcattggc agatcaagtt gtttacggcg atcttcagtg atgcacaggc aaacaatacc
    70381 gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc atggtttctg ccgggaagat
    70441 catatcgcct tcgttttcac ggtcttcatc atcaagcacc attacaccgc gtccttcacg
    70501 cagcgcagcc agtgcatttt caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt
    70561 ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac cagaatcagg gcagtcttag
    70621 gagtggcggc atatagccaa aataacgtga gcgggtccat gcccgacaga atcgttactc
    70681 tctcccatcc ggactctaac cgtcggcccc ggaattacac cggatctgct gtcctttgag
    70741 ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg tggggaattt
    70801 cgccccgccc tgagaataag cggattcact ataacgctaa tgattagcgg cagcaacgca
    70861 tagcttcaca taattctggt ttatgactta cccttatcgc actacaatgg tactcaacac
    70921 ctatcagtac agggaaacta caatgattga cccgaaaaaa attgagcaaa tcgctcgcca
    70981 ggttcacgaa tcaatgccta aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat
    71041 ccgccaaacc ctacaagctc agctgacgcg cctcgacctt gtaagccgtg aagagttcga
    71101 cgtccaaacg caagtcctgt tacggactcg tgagaaactg gcgctgcttg aacaaagaat
    71161 cagcgaactg gaaaatcgca gtactgagat caaaaagcag ccagatccag agactctccc
    71221 cccaacgctg taaacatttg tctttaatgc actgtaaatt atgaattaat cctctaattt
    71281 acagtgtttt ctattacatt atcgcttaat cccattcaga gtatcatcac aaaaatgaat
    71341 gattgcaatt gaaaatataa ttgttatgta ttctttaagt tttacttaat cgatatttag
    71401 actggccc
//
LOCUS       DAEBXD010000006        67100 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEBXD010000006 DAEBXD010000000
VERSION     DAEBXD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.006"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="KJL94_000405"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="KJL94_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBD4408901.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="KJL94_000406"
     CDS             complement(1150..1236)
                     /locus_tag="KJL94_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBD4408902.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KJL94_000407"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KJL94_000407"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD4408903.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KJL94_000408"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KJL94_000408"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBD4408904.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="KJL94_000409"
     CDS             3585..3629
                     /locus_tag="KJL94_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408905.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KJL94_000410"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KJL94_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBD4408906.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="KJL94_000411"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="KJL94_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBD4408907.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="KJL94_000412"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="KJL94_000412"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBD4408908.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="KJL94_000413"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="KJL94_000413"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBD4408909.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="KJL94_000414"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="KJL94_000414"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBD4408910.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="KJL94_000415"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="KJL94_000415"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBD4408911.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KJL94_000416"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KJL94_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBD4408912.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KJL94_000417"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KJL94_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBD4408913.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="KJL94_000418"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="KJL94_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBD4408914.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="KJL94_000419"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="KJL94_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBD4408915.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="KJL94_000420"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="KJL94_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBD4408916.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="KJL94_000421"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="KJL94_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBD4408917.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="KJL94_000422"
     CDS             complement(16981..17745)
                     /locus_tag="KJL94_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4408918.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KJL94_000423"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KJL94_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBD4408919.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="KJL94_000424"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="KJL94_000424"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBD4408920.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="KJL94_000425"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="KJL94_000425"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBD4408921.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="KJL94_000426"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="KJL94_000426"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBD4408922.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="KJL94_000427"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="KJL94_000427"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBD4408923.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="KJL94_000428"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="KJL94_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBD4408924.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="KJL94_000429"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="KJL94_000429"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBD4408925.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KJL94_000430"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KJL94_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBD4408926.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="KJL94_000431"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="KJL94_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBD4408927.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="KJL94_000432"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="KJL94_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBD4408928.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="KJL94_000433"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="KJL94_000433"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBD4408929.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="KJL94_000434"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="KJL94_000434"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBD4408930.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="KJL94_000435"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="KJL94_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBD4408931.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="KJL94_000436"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="KJL94_000436"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBD4408932.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KJL94_000437"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KJL94_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBD4408933.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KJL94_000438"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KJL94_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBD4408934.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KJL94_000439"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KJL94_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBD4408935.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="KJL94_000440"
     CDS             complement(40421..41815)
                     /locus_tag="KJL94_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4408936.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KJL94_000441"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KJL94_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBD4408937.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="KJL94_000442"
     CDS             complement(42158..43444)
                     /locus_tag="KJL94_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD4408938.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="KJL94_000443"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="KJL94_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="KJL94_000444"
     CDS             complement(44451..45221)
                     /locus_tag="KJL94_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBD4408939.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="KJL94_000445"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="KJL94_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBD4408940.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="KJL94_000446"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="KJL94_000446"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBD4408941.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="KJL94_000447"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="KJL94_000447"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBD4408942.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="KJL94_000448"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="KJL94_000448"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBD4408943.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="KJL94_000449"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="KJL94_000449"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBD4408944.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="KJL94_000450"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="KJL94_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBD4408945.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KJL94_000451"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KJL94_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBD4408946.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KJL94_000452"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KJL94_000452"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBD4408947.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KJL94_000453"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KJL94_000453"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBD4408948.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KJL94_000454"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KJL94_000454"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBD4408949.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KJL94_000455"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KJL94_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBD4408950.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KJL94_000456"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KJL94_000456"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBD4408951.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KJL94_000457"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KJL94_000457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD4408952.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KJL94_000458"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KJL94_000458"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBD4408953.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KJL94_000459"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KJL94_000459"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBD4408954.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KJL94_000460"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KJL94_000460"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBD4408955.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="KJL94_000461"
     CDS             complement(66187..66405)
                     /locus_tag="KJL94_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBD4408956.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="KJL94_000462"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="KJL94_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBD4408957.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16290 a        18259 c        17267 g        15284 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggtcgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct
//
LOCUS       DAEBXD010000007        66032 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEBXD010000007 DAEBXD010000000
VERSION     DAEBXD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66032
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.007"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="KJL94_000463"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="KJL94_000463"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBD4408958.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="KJL94_000464"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="KJL94_000464"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBD4408959.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="KJL94_000465"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="KJL94_000465"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBD4408960.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="KJL94_000466"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="KJL94_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBD4408961.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="KJL94_000467"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="KJL94_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBD4408962.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="KJL94_000468"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="KJL94_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBD4408963.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="KJL94_000469"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="KJL94_000469"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBD4408964.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="KJL94_000470"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="KJL94_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBD4408965.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="KJL94_000471"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="KJL94_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBD4408966.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="KJL94_000472"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="KJL94_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBD4408967.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="KJL94_000473"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="KJL94_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBD4408968.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="KJL94_000474"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="KJL94_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBD4408969.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="KJL94_000475"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="KJL94_000475"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBD4408970.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="KJL94_000476"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="KJL94_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBD4408971.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="KJL94_000477"
     CDS             19304..19369
                     /locus_tag="KJL94_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4408972.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="KJL94_000478"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="KJL94_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBD4408973.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="KJL94_000479"
     CDS             complement(19753..20376)
                     /locus_tag="KJL94_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBD4408974.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="KJL94_000480"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="KJL94_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBD4408975.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="KJL94_000481"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="KJL94_000481"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBD4408976.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="KJL94_000482"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="KJL94_000482"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBD4408977.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="KJL94_000483"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="KJL94_000483"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBD4408978.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="KJL94_000484"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="KJL94_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="KJL94_000485"
     CDS             complement(26460..26846)
                     /locus_tag="KJL94_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBD4408979.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="KJL94_000486"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="KJL94_000486"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBD4408980.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="KJL94_000487"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="KJL94_000487"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBD4408981.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="KJL94_000488"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="KJL94_000488"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBD4408982.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="KJL94_000489"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="KJL94_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBD4408983.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="KJL94_000490"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="KJL94_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBD4408984.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="KJL94_000491"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="KJL94_000491"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBD4408985.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="KJL94_000492"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="KJL94_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBD4408986.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="KJL94_000493"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="KJL94_000493"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBD4408987.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="KJL94_000494"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="KJL94_000494"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBD4408988.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="KJL94_000495"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="KJL94_000495"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD4408989.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="KJL94_000496"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="KJL94_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBD4408990.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="KJL94_000497"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="KJL94_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBD4408991.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="KJL94_000498"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="KJL94_000498"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBD4408992.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="KJL94_000499"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="KJL94_000499"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBD4408993.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="KJL94_000500"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="KJL94_000500"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBD4408994.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="KJL94_000501"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="KJL94_000501"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBD4408995.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="KJL94_000502"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="KJL94_000502"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBD4408996.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="KJL94_000503"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="KJL94_000503"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBD4408997.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="KJL94_000504"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="KJL94_000504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBD4408998.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="KJL94_000505"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="KJL94_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBD4408999.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="KJL94_000506"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="KJL94_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBD4409000.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="KJL94_000507"
     CDS             53914..54303
                     /locus_tag="KJL94_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD4409001.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="KJL94_000508"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="KJL94_000508"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBD4409002.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="KJL94_000509"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="KJL94_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBD4409003.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="KJL94_000510"
     CDS             complement(55962..56162)
                     /locus_tag="KJL94_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBD4409004.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="KJL94_000511"
     CDS             56328..56873
                     /locus_tag="KJL94_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBD4409005.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="KJL94_000512"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="KJL94_000512"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD4409006.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="KJL94_000513"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="KJL94_000513"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBD4409007.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKTVLLSVEGHFTLEANPDTGVPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="KJL94_000514"
     CDS             complement(58171..58995)
                     /locus_tag="KJL94_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBD4409008.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="KJL94_000515"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="KJL94_000515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBD4409009.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="KJL94_000516"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="KJL94_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBD4409010.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="KJL94_000517"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="KJL94_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBD4409011.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="KJL94_000518"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="KJL94_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBD4409012.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="KJL94_000519"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="KJL94_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBD4409013.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="KJL94_000520"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="KJL94_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBD4409014.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="KJL94_000521"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="KJL94_000521"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBD4409015.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66032
                     /locus_tag="KJL94_000522"
     rRNA            65762..>66032
                     /locus_tag="KJL94_000522"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15626 a        16347 c        18200 g        15859 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatcag gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcagcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgtggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgatagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagattcaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg ggagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gtgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctatgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacatctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaacatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctacca
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa aacggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgt gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagcaat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagt gtaatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DAEBXD010000008        61168 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEBXD010000008 DAEBXD010000000
VERSION     DAEBXD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61168
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.008"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KJL94_000523"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KJL94_000523"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBD4409016.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KJL94_000524"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KJL94_000524"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBD4409017.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KJL94_000525"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KJL94_000525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBD4409018.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KJL94_000526"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KJL94_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBD4409019.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KJL94_000527"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KJL94_000527"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBD4409020.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KJL94_000528"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KJL94_000528"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD4409021.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="KJL94_000529"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="KJL94_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBD4409022.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KJL94_000530"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KJL94_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD4409023.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KJL94_000531"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KJL94_000531"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBD4409024.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KJL94_000532"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KJL94_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBD4409025.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KJL94_000533"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KJL94_000533"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBD4409026.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="KJL94_000534"
     CDS             complement(15665..16702)
                     /locus_tag="KJL94_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD4409027.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="KJL94_000535"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="KJL94_000535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4409028.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KJL94_000536"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KJL94_000536"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBD4409029.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="KJL94_000537"
     CDS             19444..19884
                     /locus_tag="KJL94_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBD4409030.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDNSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KJL94_000538"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KJL94_000538"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD4409031.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="KJL94_000539"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="KJL94_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KJL94_000540"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KJL94_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBD4409032.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KJL94_000541"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KJL94_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBD4409033.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KJL94_000542"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KJL94_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBD4409034.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KJL94_000543"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KJL94_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBD4409035.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KJL94_000544"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KJL94_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBD4409036.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KJL94_000545"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KJL94_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBD4409037.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KJL94_000546"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KJL94_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBD4409038.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KJL94_000547"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KJL94_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBD4409039.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="KJL94_000548"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="KJL94_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBD4409040.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KJL94_000549"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KJL94_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBD4409041.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KJL94_000550"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KJL94_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBD4409042.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KJL94_000551"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KJL94_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBD4409043.1"
                     /translation="MNKRDAINHIRQFGGRLERFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KJL94_000552"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KJL94_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBD4409044.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KJL94_000553"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KJL94_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBD4409045.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="KJL94_000554"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="KJL94_000554"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBD4409046.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="KJL94_000555"
     CDS             37412..37681
                     /locus_tag="KJL94_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBD4409047.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="KJL94_000556"
     CDS             complement(37684..38043)
                     /locus_tag="KJL94_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBD4409048.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="KJL94_000557"
     CDS             38184..38810
                     /locus_tag="KJL94_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBD4409049.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="KJL94_000558"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="KJL94_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBD4409050.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KJL94_000559"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KJL94_000559"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBD4409051.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="KJL94_000560"
     CDS             41650..42315
                     /locus_tag="KJL94_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBD4409052.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="KJL94_000561"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="KJL94_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBD4409053.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="KJL94_000562"
     CDS             complement(42949..44166)
                     /locus_tag="KJL94_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4409054.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="KJL94_000563"
     CDS             44300..45349
                     /locus_tag="KJL94_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD4409055.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="KJL94_000564"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="KJL94_000564"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBD4409056.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="KJL94_000565"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="KJL94_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBD4409057.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="KJL94_000566"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="KJL94_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBD4409058.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="KJL94_000567"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="KJL94_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBD4409059.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="KJL94_000568"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="KJL94_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBD4409060.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="KJL94_000569"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="KJL94_000569"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBD4409061.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="KJL94_000570"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="KJL94_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBD4409062.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="KJL94_000571"
     CDS             complement(51544..51903)
                     /locus_tag="KJL94_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBD4409063.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="KJL94_000572"
     CDS             complement(51900..52175)
                     /locus_tag="KJL94_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBD4409064.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="KJL94_000573"
     CDS             complement(52234..53358)
                     /locus_tag="KJL94_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4409065.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KJL94_000574"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KJL94_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBD4409066.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="KJL94_000575"
     CDS             complement(56090..57157)
                     /locus_tag="KJL94_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4409067.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="KJL94_000576"
     CDS             complement(57523..59145)
                     /locus_tag="KJL94_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD4409068.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="KJL94_000577"
     CDS             complement(59407..60996)
                     /locus_tag="KJL94_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD4409069.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENCFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
BASE COUNT        14581 a        16711 c        15346 g        14530 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggataatt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgtcgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgctccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaac aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaatt
//
LOCUS       DAEBXD010000009        60383 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEBXD010000009 DAEBXD010000000
VERSION     DAEBXD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60383
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.009"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..322
                     /locus_tag="KJL94_000578"
     CDS             <1..322
                     /locus_tag="KJL94_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4409070.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="KJL94_000579"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="KJL94_000579"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBD4409071.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="KJL94_000580"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="KJL94_000580"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBD4409072.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KJL94_000581"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KJL94_000581"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBD4409073.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="KJL94_000582"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="KJL94_000582"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBD4409074.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="KJL94_000583"
     CDS             complement(3726..4955)
                     /locus_tag="KJL94_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD4409075.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="KJL94_000584"
     CDS             complement(5019..5459)
                     /locus_tag="KJL94_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD4409076.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="KJL94_000585"
     CDS             complement(5564..6334)
                     /locus_tag="KJL94_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4409077.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="KJL94_000586"
     CDS             complement(6331..7257)
                     /locus_tag="KJL94_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4409078.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="KJL94_000587"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="KJL94_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBD4409079.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KJL94_000588"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KJL94_000588"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBD4409080.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="KJL94_000589"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="KJL94_000589"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBD4409081.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KJL94_000590"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KJL94_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBD4409082.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="KJL94_000591"
     CDS             12964..13311
                     /locus_tag="KJL94_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBD4409083.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="KJL94_000592"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="KJL94_000592"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBD4409084.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="KJL94_000593"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="KJL94_000593"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBD4409085.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="KJL94_000594"
     CDS             complement(15131..15493)
                     /locus_tag="KJL94_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBD4409086.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KJL94_000595"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KJL94_000595"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBD4409087.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="KJL94_000596"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="KJL94_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KJL94_000597"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KJL94_000597"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBD4409088.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24137)
                     /gene="aceF"
                     /locus_tag="KJL94_000598"
     CDS             complement(22293..24137)
                     /gene="aceF"
                     /locus_tag="KJL94_000598"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBD4409089.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGT
                     GRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRI
                     QKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKA
                     VAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSR
                     ELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEP
                     VWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM"
     gene            complement(24152..26815)
                     /gene="aceE"
                     /locus_tag="KJL94_000599"
     CDS             complement(24152..26815)
                     /gene="aceE"
                     /locus_tag="KJL94_000599"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBD4409090.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26976..27740)
                     /gene="pdhR"
                     /locus_tag="KJL94_000600"
     CDS             complement(26976..27740)
                     /gene="pdhR"
                     /locus_tag="KJL94_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBD4409091.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28281..29654
                     /gene="aroP"
                     /locus_tag="KJL94_000601"
     CDS             28281..29654
                     /gene="aroP"
                     /locus_tag="KJL94_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBD4409092.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29697..30551)
                     /gene="ampE"
                     /locus_tag="KJL94_000602"
     CDS             complement(29697..30551)
                     /gene="ampE"
                     /locus_tag="KJL94_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBD4409093.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30548..31099)
                     /gene="ampD"
                     /locus_tag="KJL94_000603"
     CDS             complement(30548..31099)
                     /gene="ampD"
                     /locus_tag="KJL94_000603"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBD4409094.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31187..32080
                     /gene="nadC"
                     /locus_tag="KJL94_000604"
     CDS             31187..32080
                     /gene="nadC"
                     /locus_tag="KJL94_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBD4409095.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32283..32723
                     /gene="ppdD"
                     /locus_tag="KJL94_000605"
     CDS             32283..32723
                     /gene="ppdD"
                     /locus_tag="KJL94_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBD4409096.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32733..33880
                     /gene="tadA"
                     /locus_tag="KJL94_000606"
                     /pseudo
     CDS             32733..33880
                     /gene="tadA"
                     /locus_tag="KJL94_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33870..35072
                     /gene="hofC"
                     /locus_tag="KJL94_000607"
     CDS             33870..35072
                     /gene="hofC"
                     /locus_tag="KJL94_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBD4409097.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35107..36150)
                     /gene="guaC"
                     /locus_tag="KJL94_000608"
     CDS             complement(35107..36150)
                     /gene="guaC"
                     /locus_tag="KJL94_000608"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBD4409098.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36312..36350)
                     /locus_tag="KJL94_000609"
     CDS             complement(36312..36350)
                     /locus_tag="KJL94_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409099.1"
                     /translation="MIIKTLKMSARK"
     gene            36375..36995
                     /gene="coaE"
                     /locus_tag="KJL94_000610"
     CDS             36375..36995
                     /gene="coaE"
                     /locus_tag="KJL94_000610"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBD4409100.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36995..37738
                     /gene="zapD"
                     /locus_tag="KJL94_000611"
     CDS             36995..37738
                     /gene="zapD"
                     /locus_tag="KJL94_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBD4409101.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37748..37945
                     /gene="yacG"
                     /locus_tag="KJL94_000612"
     CDS             37748..37945
                     /gene="yacG"
                     /locus_tag="KJL94_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBD4409102.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38045..38434)
                     /gene="mutT"
                     /locus_tag="KJL94_000613"
     CDS             complement(38045..38434)
                     /gene="mutT"
                     /locus_tag="KJL94_000613"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBD4409103.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38494..41199)
                     /gene="secA"
                     /locus_tag="KJL94_000614"
     CDS             complement(38494..41199)
                     /gene="secA"
                     /locus_tag="KJL94_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBD4409104.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41261..41773)
                     /gene="secM"
                     /locus_tag="KJL94_000615"
     CDS             complement(41261..41773)
                     /gene="secM"
                     /locus_tag="KJL94_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBD4409105.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42004..42921)
                     /gene="lpxC"
                     /locus_tag="KJL94_000616"
     CDS             complement(42004..42921)
                     /gene="lpxC"
                     /locus_tag="KJL94_000616"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBD4409106.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43022..44173)
                     /gene="ftsZ"
                     /locus_tag="KJL94_000617"
     CDS             complement(43022..44173)
                     /gene="ftsZ"
                     /locus_tag="KJL94_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBD4409107.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44234..45496)
                     /gene="ftsA"
                     /locus_tag="KJL94_000618"
     CDS             complement(44234..45496)
                     /gene="ftsA"
                     /locus_tag="KJL94_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBD4409108.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45493..46323)
                     /gene="ftsQ"
                     /locus_tag="KJL94_000619"
     CDS             complement(45493..46323)
                     /gene="ftsQ"
                     /locus_tag="KJL94_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBD4409109.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46325..47245)
                     /gene="ddlB"
                     /locus_tag="KJL94_000620"
     CDS             complement(46325..47245)
                     /gene="ddlB"
                     /locus_tag="KJL94_000620"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD4409110.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPIDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47238..48713)
                     /gene="murC"
                     /locus_tag="KJL94_000621"
     CDS             complement(47238..48713)
                     /gene="murC"
                     /locus_tag="KJL94_000621"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBD4409111.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48767..49834)
                     /gene="murG"
                     /locus_tag="KJL94_000622"
     CDS             complement(48767..49834)
                     /gene="murG"
                     /locus_tag="KJL94_000622"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBD4409112.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49831..51075)
                     /gene="ftsW"
                     /locus_tag="KJL94_000623"
     CDS             complement(49831..51075)
                     /gene="ftsW"
                     /locus_tag="KJL94_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBD4409113.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51075..52391)
                     /gene="murD"
                     /locus_tag="KJL94_000624"
     CDS             complement(51075..52391)
                     /gene="murD"
                     /locus_tag="KJL94_000624"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBD4409114.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52394..53476)
                     /gene="mraY"
                     /locus_tag="KJL94_000625"
     CDS             complement(52394..53476)
                     /gene="mraY"
                     /locus_tag="KJL94_000625"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBD4409115.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53470..54828)
                     /gene="murF"
                     /locus_tag="KJL94_000626"
     CDS             complement(53470..54828)
                     /gene="murF"
                     /locus_tag="KJL94_000626"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBD4409116.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54825..56312)
                     /gene="murE"
                     /locus_tag="KJL94_000627"
     CDS             complement(54825..56312)
                     /gene="murE"
                     /locus_tag="KJL94_000627"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBD4409117.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56299..58065)
                     /gene="ftsI"
                     /locus_tag="KJL94_000628"
     CDS             complement(56299..58065)
                     /gene="ftsI"
                     /locus_tag="KJL94_000628"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBD4409118.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58081..58446)
                     /gene="ftsL"
                     /locus_tag="KJL94_000629"
     CDS             complement(58081..58446)
                     /gene="ftsL"
                     /locus_tag="KJL94_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBD4409119.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58443..59384)
                     /gene="rsmH"
                     /locus_tag="KJL94_000630"
     CDS             complement(58443..59384)
                     /gene="rsmH"
                     /locus_tag="KJL94_000630"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBD4409120.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59386..59844)
                     /gene="mraZ"
                     /locus_tag="KJL94_000631"
     CDS             complement(59386..59844)
                     /gene="mraZ"
                     /locus_tag="KJL94_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBD4409121.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16719 c        14836 g        14356 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgaacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagcg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg cgccttcaac ttcgaagatc ataatcagcg aaccagtttt cactttatcg
    23341 ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccggaac ttccatagaa
    23401 gctttatcgc cttctacggt aatcagtgac tgttcagcgg caactttgtc gcccactttc
    23461 accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt aacttctttc
    23521 acgccagctg ctggtgcagg ggtcgctgcc ggagtcgctt cctgtttagc cgccggagct
    23581 gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt agacactttg
    23641 tcacccacgt tcactttgat ctctttcacg gtacctgcaa acggagccgg aacttccata
    23701 gaagccttgt cgccttctac ggtgatcagc gactgttcag cttcaacttt atcgccaact
    23761 ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac gttaacgtct
    23821 tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc ttctgcctga
    23881 gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag tgcgccggtc
    23941 tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg cggagatgga
    24001 acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc ttcaactttg
    24061 tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat gtccggtact
    24121 ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta actttatctg
    24181 catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt tcgccacgtt
    24241 tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg aagtggtgac
    24301 gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg cgatagtcgt
    24361 cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg gtagatgcca
    24421 ctgccggcgc gtcgttcatc acctgagcga tatacggaac gcgcggagtt tccagcggat
    24481 gcagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg aaggaggtca
    24541 cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc gcttcacgga
    24601 cgtgacgcag gatggaaccg gagcccagca gctgaacttt acctttgcta ccttcaatgg
    24661 tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc attgccggca
    24721 tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt ttttcaccgt
    24781 acatacgctc cagaccgtca tgcataatga cagcaacttc gtaagcgtaa gccgggtcgt
    24841 aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct tcgtgctgca
    24901 gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag ccacgcgctt
    24961 gctggtctcc agccgcccag cacagatcgc cgatacgctg gaagccgaac atcgagtaat
    25021 agatgtagaa tgggatcatc ggcagattgt tcgtgctgta agaggtcgcc gctgccagcc
    25081 aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct ttctcgtctt
    25141 ctttatagta agcaacctgc tcgcggtcct gcggggtgta ctgctgaccg ttcgggctgt
    25201 aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg tcggcgatga
    25261 tcggtaccag acgatctttg atcgacttgt tcttcagcat cacgttcaga gcacgaacga
    25321 aagcgatagt ggtagagagc tctttgctct gctcttccag cagcgcgccg aagtcttgca
    25381 ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga taaccgtgca
    25441 gtttctgacg ctgagcgtgc agataggtat gctcttcaga accttccggg aaggtgatgt
    25501 acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg cggatgtgac
    25561 gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct tcagccgcgt
    25621 cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg cctttggttt
    25681 cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca cggttcagtg
    25741 cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga tatttaccga
    25801 agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag tcgccgtcaa
    25861 cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc agttcatccc
    25921 aacggctacc ccacatcact ttgatcacgt tccagccagc accttcgaag atgccttcca
    25981 gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc aggttacagt
    26041 tgataacgaa aaccaggtta tccagttttt cacgggtagc gatggtgatc gcacctttgg
    26101 attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt ttagaggtat
    26161 ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata gcaccaatcg
    26221 gacccagacc catagatacg gtcgggaact gccagaattc cggcatcagt ttcgggtgcg
    26281 gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc tgctcctgag
    26341 tcagacgacc ttccaggaaa gcacgggcgt aaacgcccgg ggagatgtgg ccctggaagt
    26401 aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta aagcacacat
    26461 cataaatggt tgcggaagac tggaaggacg ccatgtggcc gcccagttcg aggtcttttt
    26521 tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga atacggcgtt
    26581 ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg ttgatgtagt
    26641 tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca gcaagcagtt
    26701 ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat tcgatcgcct
    26761 ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct gacatgggtt
    26821 attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga actctcgcca
    26881 gaaagctcaa taagacaggt tctacgttta gttaccgcgc ttttatatgc gcttgattta
    26941 caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg ctccagacga
    27001 cgcagagaac gctcacggcg gctctcttca cggcttctgt cgagcaaaat ttcttcgata
    27061 aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc cataatcgct
    27121 tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg cgaatagagc
    27181 aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct tagcagatga
    27241 agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag tacggcgttt
    27301 gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg gagttcacgg
    27361 atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc gataccttcc
    27421 agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga gagcagctcc
    27481 accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc gccaccctgg
    27541 cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa ggagggacgg
    27601 gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc cgggcggaga
    27661 gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca catcggagag ttttggttgg
    27721 cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt caattggtct
    27781 taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt ccatacaggt
    27841 tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata acgtttcaaa
    27901 gttgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa cgattattaa
    27961 actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt aagtcaataa
    28021 aaacgaattt aaattcactc tgcatattga gaggggttga ggctgagctt tacaaacggt
    28081 ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc tgccgcatac
    28141 cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcctt gatgtaaata
    28201 aatcaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa
    28261 caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg
    28321 gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg acagggctat
    28381 tcctaggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
    28441 ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg gtcgaagaac
    28501 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
    28561 cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag ctgactgccg
    28621 tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct gccgccgtat
    28681 tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt
    28741 tctggtttgc cattatcaaa gttattgcgg tagtagcgat gatcatcttc ggcggctggc
    28801 tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg gatcaaggtg
    28861 gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc atgttctcgt
    28921 tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg gagcaaagta
    28981 tcccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag
    29041 ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc
    29101 tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc gtggtactga
    29161 ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc
    29221 tggcacaaca ggggaacgcg ccaaaagcgc tggcgtctgt cgataaacgc ggcgtaccgg
    29281 ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt aactatcttg
    29341 ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg gtgatcaact
    29401 ggacgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa caaggcgtgg
    29461 taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
    29521 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
    29581 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
    29641 taaaagcgca ttaatctctc tacgccctca cccgtacagg gtgagggcaa taatctctac
    29701 accaacgccc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa cgaggttttc
    29761 ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg ttcacgcgcc
    29821 agagagaact gcgccagacg cgttaatacc tgatattgcg aagtatggaa atcacccagc
    29881 gaagcaaacc aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc
    29941 gcaagacgaa ccggcaccca atccagtaca tgaagcactg catcaatgcc ggactgtaaa
    30001 cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa gaacgcatac
    30061 cccatcagcg taacgggtcc ccaggttccc cccacaatca gccagaacag cggtgcaaga
    30121 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttcg
    30181 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca
    30241 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
    30301 gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac gttgaacaat
    30361 actccctgca atgcgcgtaa cagtaaaaaa gtcacaccca tcgcaataat ggtcatgcct
    30421 aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
    30481 agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
    30541 aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa tcaaatgcag
    30601 gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg ttattagcga
    30661 tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga tactgcgcat
    30721 cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa tcattgcagc
    30781 gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg aaaggaacat
    30841 actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
    30901 tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat gcgtcgatcc
    30961 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
    31021 tttcgtcatc caggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc
    31081 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
    31141 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
    31201 ctacaaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
    31261 cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgacat
    31321 tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc acggtgatca cccgcgagaa
    31381 tggcgtattt tgtggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
    31441 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc aacccttgtt
    31501 cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt
    31561 gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac tggaaggcac
    31621 caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag ctctgaaata
    31681 cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat
    31741 caaagaaaac catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg
    31801 gctgcatccg gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaggc
    31861 cctgaaagcc ggagccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
    31921 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
    31981 aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa
    32041 acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag atttatccgc
    32101 tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct cgcaaatttc
    32161 ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg ctttcccgtt tcgttttttg
    32221 cccctcgcct gtgctgatgg cgtttgccaa agtagcacca accaaatcaa ggagcgaaac
    32281 agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg
    32341 ccattttaag ctccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca
    32401 ccgacatgct acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac
    32461 atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc
    32521 gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc gctgaccggg caagaaagtc
    32581 tcaatgggct aagcgtcgtc atgacgccag gttgggataa cgcaaacggc gtcaccggct
    32641 gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc gaagatgtct
    32701 tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc acggccctgt
    32761 gcctgcgtta tcagggagtc ttgctggatg ccagcgaaga agtggttcat gttgcggtgg
    32821 tcgatgcccc ctcacatgag ttgctggacg cattgcattt cgctaccacc aaacgtattg
    32881 agatcacctg ctggacgcgc caacaaatgg aaggtcacgc cagtcgcaca caacagacat
    32941 tacccgtagc tgttcaggag aagcatcagc ccaaagcaga attgctgact cgaacgttac
    33001 aatctgcgct ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc
    33061 gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca ccggatgccg
    33121 gagtcgcatt aacagccaga ttaaaagtgc tgggaaacct ggatattgcg gaacatcgcc
    33181 tgccgcagga cgggcaattc actgtcgaac tggcaggaaa caccgtctca tttcgtattg
    33241 cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagcc
    33301 aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac tttgctcatg
    33361 ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagt ggcaaaacgg
    33421 tcacctggtg ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca
    33481 gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg
    33541 tctggtacgt aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc
    33601 agacactgta tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta
    33661 ccacggtttt tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg
    33721 ccagcttatt tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg
    33781 tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga
    33841 agagttaatc cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg
    33901 gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac
    33961 tgcttatggc actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca
    34021 attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac
    34081 ttaaagccgg gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta
    34141 agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt
    34201 ccaatgcctt attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca
    34261 cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat
    34321 cccagcgtca gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag
    34381 cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta
    34441 tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag
    34501 actttagtgg cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca
    34561 ataagttgct gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca
    34621 tcccgattat gggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg
    34681 cgctgacaca aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa
    34741 tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
    34801 atcccatctg gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag
    34861 tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc
    34921 atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc
    34981 tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc
    35041 atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt
    35101 gggagattac aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt
    35161 gcgcttggtc agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
    35221 gcccaaaata tcacgcgcgg tattttcaac ctggcctcgc agcggcagct taacggtttt
    35281 accttctgct gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga
    35341 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
    35401 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
    35461 tttcgccaca tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc
    35521 gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt
    35581 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
    35641 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
    35701 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
    35761 cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
    35821 gaaatccgca tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga
    35881 attgttgata aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat
    35941 atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc
    36001 gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag
    36061 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
    36121 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
    36181 ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc
    36241 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
    36301 tttcatcaat ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc
    36361 cgggaatata aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga
    36421 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
    36481 cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg
    36541 aaaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
    36601 ccaacccgga agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga
    36661 cgcaacacca gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctgg
    36721 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
    36781 cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc
    36841 ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata
    36901 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
    36961 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
    37021 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
    37081 cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga
    37141 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg
    37201 gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga
    37261 agcattaatt cagcagttaa aagcggcggt gagcgtatta atttccgcgc cgcgtatcgg
    37321 gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg
    37381 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca
    37441 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac
    37501 catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg
    37561 tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc
    37621 acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct
    37681 ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg
    37741 agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
    37801 gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg
    37861 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
    37921 ctggagcgaa gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac
    37981 gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc
    38041 cgacctacag acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg
    38101 cattaagacc gaccagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc
    38161 cttcccaact ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt
    38221 ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac
    38281 gcaccaccgc ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt
    38341 tattcgccat gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc
    38401 gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca
    38461 gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt
    38521 gtatttttta ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt
    38581 ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat
    38641 ttgtgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg
    38701 catacgtacc tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat
    38761 cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata
    38821 gccacgcagg tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag
    38881 ggagtcaagc gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac
    38941 cacttcttct ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt
    39001 ctcttcatgc agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa
    39061 atcgttcttc agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg
    39121 cggaatgtag gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc
    39181 gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc
    39241 gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac
    39301 tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg
    39361 cttcataccc agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag
    39421 cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg
    39481 accgcgcaac tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag
    39541 gccacctgct tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat
    39601 ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac
    39661 aatatctgta ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc
    39721 ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat
    39781 accggctttg gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac
    39841 cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt
    39901 ttccgcttca gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt
    39961 cggaacaacg acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc
    40021 agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga
    40081 agccagcgtt tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg
    40141 cagaccatcg gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat
    40201 aacttcacca tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag
    40261 cgccgccgtt acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc
    40321 atccatgatg ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag
    40381 gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga
    40441 gtcttctttt tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc
    40501 cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat
    40561 ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct
    40621 gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat
    40681 gtcagctgcg taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac
    40741 agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta
    40801 gtcgttgacg gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag
    40861 cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag
    40921 aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc
    40981 ctcacgcacc acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag
    41041 acgcgcacga aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc
    41101 cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt
    41161 acgactaccg aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg
    41221 cgttacggag ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc
    41281 cagcacggat gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg
    41341 taaaatgcgc ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca
    41401 gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag
    41461 caacgggcag tgtttgcggt gccattgcga aagaaagatg acggattacc gtacgaatag
    41521 catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca
    41581 gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt
    41641 ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga
    41701 ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca
    41761 gtattccact cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct
    41821 tatcattaaa gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta
    41881 agaaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg
    41941 ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg
    42001 tcgttatgcc agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg
    42061 gaaggtcaca tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt
    42121 cagtgcatga ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa
    42181 caagtcaccg atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc
    42241 gtcttcgttc agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag
    42301 gcacaaacca cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg
    42361 gctgatctgg cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga
    42421 atcaatagcc ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt
    42481 aaattcagcc cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt
    42541 tttggcgcag ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc
    42601 gctgccgtcc atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc
    42661 caggcccgcg agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt
    42721 gaccagacac gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac
    42781 cggtggattc aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa
    42841 cgtcagggtg actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt
    42901 aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta
    42961 ctatacattc ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt
    43021 cttaatcagc ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag
    43081 tttgcggcgc attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag
    43141 ccatcccatg ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca
    43201 gagtgatttc aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct
    43261 cgtcattcat atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa
    43321 atgcacggat ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg
    43381 tgatgttaac cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag
    43441 agatagccat ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca
    43501 tcattgcgta gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca
    43561 aacccggacg agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg
    43621 ctgcgccaaa cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt
    43681 tcgggatagt gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga
    43741 atgccatacg cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga
    43801 tacccaaatc ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac
    43861 ccatacccgc agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc
    43921 gatcctcatc agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg
    43981 tgataccgct accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat
    44041 cggtatttac cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag
    44101 cattaccgcc gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca
    44161 ttggttcaaa catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc
    44221 ggcctcataa aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc
    44281 caactgatgc tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc
    44341 catagtgaag caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac
    44401 cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg
    44461 ctgcaagacc ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat
    44521 gtttaacccc ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt
    44581 tgagcaactc ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc
    44641 gcggcggacg accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac
    44701 ccagcgcaca accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg
    44761 cgtaagcgat atcactggtc acgacattgc cagcataagg aattaccttt gtgtggcgca
    44821 atgccccacc ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga
    44881 cacccagttc acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata
    44941 tcagttggtc aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat
    45001 cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg
    45061 gattcttgat cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac
    45121 gatgctcatc gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt
    45181 gcgtcacttc ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct
    45241 taccagaaag cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt
    45301 caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca
    45361 taccacgtga cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt
    45421 cccctactaa agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt
    45481 ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc
    45541 tggcggcaag ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct
    45601 aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg
    45661 aaccaaacgt ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt
    45721 caactgccag gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc
    45781 cagcatctgc cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc
    45841 atacagcatt ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc
    45901 ttccgcgtct accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg
    45961 aatcttcaat tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg
    46021 ttgttctatt tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc
    46081 caatgccagg atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa
    46141 caccagcttt gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc
    46201 gctcaccaac actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc
    46261 attattgcgg cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga
    46321 catattagtc cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct
    46381 gacgtgccgc catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca
    46441 gcagataaaa ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac
    46501 ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca
    46561 gacctgcggg gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga
    46621 aggttccgga cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg
    46681 tgaactccgg cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca
    46741 atcttaatgc atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac
    46801 cttcgcggct cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct
    46861 tatcgctcag gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta
    46921 aaccggcacc ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca
    46981 tcactccgct gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat
    47041 cttcaccgcc gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt
    47101 gcgtcacgtc gacttctttc gggtcgatag gatacgcatc aataccgcct tcacgcagcc
    47161 cggctaacac cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca
    47221 acaggaccgc gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg
    47281 atttcagcta aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg
    47341 tcgttaccgg ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga
    47401 atgggatcaa ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc
    47461 ggaattggcg cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc
    47521 agcacattgg cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac
    47581 agcattacca ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact
    47641 tccgtcgggt ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact
    47701 ggctccagcg ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc
    47761 agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct
    47821 gcggcgttca gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg
    47881 tcctgacgca gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc
    47941 acgtcggcat cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat
    48001 tcgcggatca ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc
    48061 agaaaattaa taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg
    48121 tcggcttcga tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca
    48181 ctctcatctg cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc
    48241 gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg
    48301 gaaaccatcg cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga
    48361 aaacgcatta actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga
    48421 gctgcgacaa tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca
    48481 tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc
    48541 tgcgtgaccg gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga
    48601 acttcggcaa taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg
    48661 cgacgcattt cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac
    48721 gccattaact tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag
    48781 cccggctcac ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc
    48841 gttctgccat ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat
    48901 ccacgctaag ctgtggctgc tcaataattt tggctgcgcc cgctttttcc agcggtagcg
    48961 cattccagta ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg
    49021 ccgcggcgat ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg
    49081 catacgccgc cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt
    49141 cggcatacgc ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga
    49201 ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct
    49261 gggaaccacc cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg
    49321 gcaacggcag cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga
    49381 aagcgcctgg aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta
    49441 agcccgcaat accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca
    49501 gaccacctgg acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt
    49561 tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg
    49621 cttttatacc ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt
    49681 ttggcactaa gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct
    49741 gagccattag atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg
    49801 ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc
    49861 tttctccaga cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat
    49921 cagtaagctc gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat
    49981 ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca
    50041 ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc
    50101 acgaaaagcg acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc
    50161 ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg
    50221 taccgaatta cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg
    50281 cgttaactga tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg
    50341 gcggatacgg tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat
    50401 aatggcaatg aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac
    50461 aaacaacacc accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag
    50521 aatcacgccc atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg
    50581 caccagatag ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat
    50641 acgcagcaaa ccgagatcga tccaacgcga tgccccttta acagcgctac ccactaccag
    50701 cacgatcatc agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa
    50761 ctccatcggc agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc
    50821 atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc
    50881 cgaggtcacc atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg
    50941 atcgtacatg atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag
    51001 cgccgtggag atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg
    51061 gagagataaa cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt
    51121 tcaaagttct tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc
    51181 ggctgaacac gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc
    51241 acttccgggc gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg
    51301 ttatcgccat tcaggtaacg caccagtggg ctaaagtccg ccgatttacc atcgccgccc
    51361 agcaacaaat gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg
    51421 acgttggtcg cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca
    51481 aagcgatgcg gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac
    51541 cctgcggcat ctgccagcgc cagcgccacc agcgcattgg tgtagttatg ctgcccggaa
    51601 agtttcatct ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc
    51661 tgctgatgat tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc
    51721 gcaccgcgaa tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt
    51781 tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata
    51841 tgatcttcag tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc
    51901 agctggaagc tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac
    51961 gcaggcaggc caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca
    52021 cccactagcg tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct
    52081 tgtgcttcgc gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca
    52141 gcgcttaagg atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc
    52201 atcagccatt catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc
    52261 aggccaggcg gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa
    52321 aagtccacgc aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg
    52381 taatcagcca tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg
    52441 aaataatcca gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt
    52501 gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca
    52561 ggatgacaga aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct
    52621 gacgtagcag tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc
    52681 ccataaagac ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta
    52741 tcgcggtaca gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc
    52801 tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata
    52861 cggtcggcat aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac
    52921 ccacaatgac gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt
    52981 taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga
    53041 aagcaacacc cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt
    53101 tggtgtcttt acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta
    53161 ccagcaccac caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg
    53221 agatcacaat cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga
    53281 agtgtgattc aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag
    53341 caatcatgcg cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg
    53401 cgcgaaacgt cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg
    53461 ccagccaaac taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc
    53521 actacgtgaa cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag
    53581 acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat
    53641 agcatggctt tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc
    53701 acccacctgt acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac
    53761 cagcacgcgg tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac
    53821 attggcgttg taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag
    53881 acggccagga acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc
    53941 cacggacatg gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa
    54001 cggcagcaga acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga
    54061 ggtcacatgg atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca
    54121 cactttgcgt gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat
    54181 aatggcgata ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc
    54241 cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc
    54301 cgggcgagtc agactcacag tccaggcaat ctcgccctga tggttcgcgc caagttcaat
    54361 aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt
    54421 gttgagattg cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat
    54481 ctctttaacg gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg
    54541 ctggcgaacc catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg
    54601 cggcaggtcg atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc
    54661 ctggtcggca aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc
    54721 cggcgtcagt tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag
    54781 ttcaccgttg agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc
    54841 agcagacgcg ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg
    54901 taatcctcat ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg
    54961 gcgcaagtca ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt
    55021 cccgccagaa tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc
    55081 accaccgcca cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta
    55141 tcgcgatcgc caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc
    55201 gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt
    55261 ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt
    55321 ttcagcagat cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg
    55381 ttgctgacgt taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa
    55441 ctaaagcgaa tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt
    55501 ccgtgacagt tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt
    55561 gccagccagc ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt
    55621 tcagaataaa gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg
    55681 tgatcgcggc ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc
    55741 tgtaccagcc cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc
    55801 cccgccagct catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact
    55861 ttccccagca gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg
    55921 ctccactgcg ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg
    55981 agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg
    56041 ttgagctggc tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc
    56101 gcctcatctt tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata
    56161 tatcgacgcc cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc
    56221 acacagctgt cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga
    56281 gcaagaaggt cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa
    56341 ttcattttta tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc
    56401 gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg
    56461 cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc
    56521 ggtataagca atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc
    56581 ggttttaatg gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag
    56641 cgccacgctt tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc
    56701 accgggaacc ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc
    56761 gatagttgcg tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga
    56821 gaaggtggcc ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc
    56881 tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa
    56941 cgctgaggac ggcatcgtta acgccagctt ggaaacaccg acgttactcg acttctgtaa
    57001 taccccggtc agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat
    57061 tcgataagga atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt
    57121 catcaccacc atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt
    57181 acgcatcgac tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc
    57241 catcgccagc acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt
    57301 gttaaaggcc accgcgttgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat
    57361 actcagcgcc aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg
    57421 accatagcgg tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa
    57481 actcttctca acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc
    57541 agtcacttcg ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag
    57601 tttcagtttt ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa
    57661 gcgccctttc gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc
    57721 gttagccagc gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt
    57781 cgggtcagcc caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc
    57841 agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc
    57901 tttcaccagc atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc
    57961 cagcgccagg agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg
    58021 ttcttcctga cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc
    58081 ttatttttgc actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc
    58141 cgtggcgatc ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt
    58201 gcgccattca atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag
    58261 taaacgggta tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa
    58321 caggcagagt ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc
    58381 atggcgctcg tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct
    58441 gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca
    58501 gccacctctt cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt
    58561 tttttgagct gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt
    58621 tcacgcataa aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc
    58681 cgcccacccg gggccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt
    58741 tcactgttta cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta
    58801 tctttcaccg gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc
    58861 tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaatg ctcttcacca
    58921 taggttttca atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct
    58981 gactgcccac gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag
    59041 ccgcgttcag catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg
    59101 atcttgccaa taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg
    59161 atgatggaga agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga
    59221 tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca
    59281 ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg
    59341 gcttcatcca gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc
    59401 ctgcagtcgc tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac
    59461 ctgttgatgc caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat
    59521 cacttctttt gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc
    59581 gccatccatc tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac
    59641 cgggttcatg ctcaacagac gcgataattt ttgctcgata atttcccatt caggcagggg
    59701 gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa accatttgac cggcagcgtt
    59761 ctcaagcagc tgttcccgat aacgggtagg cactgataag cgccctttgc tgtcgagatt
    59821 gactaacgtt gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa
    59881 tttcccactg aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca
    59941 taccgaggta aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa
    60001 cgtgcgaagc aaattaacgt tatgaatagc tgtaagaaaa aatccctcat cgtcatcact
    60061 gttttaaaat gactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc
    60121 ggcattttag gacatatctt ccccggttaa cagtccttgt tacgtctgtg tggcgcttga
    60181 tgccagcggg tttgcgcaaa agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg
    60241 catgtaacaa aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag
    60301 ctggtgagtt aattaacggg cgggtttaaa accatttatc aataatagtc aggaatagtc
    60361 ttatttactt taaggatatt gat
//
LOCUS       DAEBXD010000010        59858 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEBXD010000010 DAEBXD010000000
VERSION     DAEBXD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59858
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.010"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            549..1838
                     /gene="sdaC"
                     /locus_tag="KJL94_000632"
     CDS             549..1838
                     /gene="sdaC"
                     /locus_tag="KJL94_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBD4409122.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1896..3263
                     /gene="sdaB"
                     /locus_tag="KJL94_000633"
     CDS             1896..3263
                     /gene="sdaB"
                     /locus_tag="KJL94_000633"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBD4409123.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3374..4129
                     /gene="xni"
                     /locus_tag="KJL94_000634"
     CDS             3374..4129
                     /gene="xni"
                     /locus_tag="KJL94_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBD4409124.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4182..5330)
                     /gene="fucO"
                     /locus_tag="KJL94_000635"
     CDS             complement(4182..5330)
                     /gene="fucO"
                     /locus_tag="KJL94_000635"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBD4409125.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5358..6005)
                     /gene="fucA"
                     /locus_tag="KJL94_000636"
     CDS             complement(5358..6005)
                     /gene="fucA"
                     /locus_tag="KJL94_000636"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBD4409126.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6552..7868
                     /gene="fucP"
                     /locus_tag="KJL94_000637"
     CDS             6552..7868
                     /gene="fucP"
                     /locus_tag="KJL94_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBD4409127.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7901..9676
                     /gene="fucI"
                     /locus_tag="KJL94_000638"
     CDS             7901..9676
                     /gene="fucI"
                     /locus_tag="KJL94_000638"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBD4409128.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9785..11204
                     /gene="fucK"
                     /locus_tag="KJL94_000639"
                     /pseudo
     CDS             9785..11204
                     /gene="fucK"
                     /locus_tag="KJL94_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11206..11628
                     /gene="fucU"
                     /locus_tag="KJL94_000640"
     CDS             11206..11628
                     /gene="fucU"
                     /locus_tag="KJL94_000640"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBD4409129.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11686..12417
                     /gene="fucR"
                     /locus_tag="KJL94_000641"
     CDS             11686..12417
                     /gene="fucR"
                     /locus_tag="KJL94_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBD4409130.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12461..13561)
                     /gene="rlmM"
                     /locus_tag="KJL94_000642"
     CDS             complement(12461..13561)
                     /gene="rlmM"
                     /locus_tag="KJL94_000642"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBD4409131.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13554..13949)
                     /locus_tag="KJL94_000643"
     CDS             complement(13554..13949)
                     /locus_tag="KJL94_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBD4409132.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13968..14885)
                     /gene="gcvA"
                     /locus_tag="KJL94_000644"
     CDS             complement(13968..14885)
                     /gene="gcvA"
                     /locus_tag="KJL94_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBD4409133.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15236..15463)
                     /locus_tag="KJL94_000645"
     CDS             complement(15236..15463)
                     /locus_tag="KJL94_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBD4409134.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15655..16860
                     /gene="csdA"
                     /locus_tag="KJL94_000646"
     CDS             15655..16860
                     /gene="csdA"
                     /locus_tag="KJL94_000646"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBD4409135.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16860..17303
                     /gene="csdE"
                     /locus_tag="KJL94_000647"
     CDS             16860..17303
                     /gene="csdE"
                     /locus_tag="KJL94_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBD4409136.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17354..18160)
                     /gene="tcdA"
                     /locus_tag="KJL94_000648"
     CDS             complement(17354..18160)
                     /gene="tcdA"
                     /locus_tag="KJL94_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBD4409137.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18399..19496)
                     /gene="mltA"
                     /locus_tag="KJL94_000649"
     CDS             complement(18399..19496)
                     /gene="mltA"
                     /locus_tag="KJL94_000649"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBD4409138.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19705..19781
                     /locus_tag="KJL94_000650"
     tRNA            19705..19781
                     /locus_tag="KJL94_000650"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19739..19741,aa:Met,seq:cat)
     gene            19815..19891
                     /locus_tag="KJL94_000651"
     tRNA            19815..19891
                     /locus_tag="KJL94_000651"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19849..19851,aa:Met,seq:cat)
     gene            complement(19966..21219)
                     /gene="amiC"
                     /locus_tag="KJL94_000652"
     CDS             complement(19966..21219)
                     /gene="amiC"
                     /locus_tag="KJL94_000652"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBD4409139.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21451..22782
                     /gene="argA"
                     /locus_tag="KJL94_000653"
     CDS             21451..22782
                     /gene="argA"
                     /locus_tag="KJL94_000653"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBD4409140.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22844..24670)
                     /gene="recD"
                     /locus_tag="KJL94_000654"
     CDS             complement(22844..24670)
                     /gene="recD"
                     /locus_tag="KJL94_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBD4409141.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24670..28212)
                     /gene="recB"
                     /locus_tag="KJL94_000655"
     CDS             complement(24670..28212)
                     /gene="recB"
                     /locus_tag="KJL94_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBD4409142.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28205..31093)
                     /gene="ptrA"
                     /locus_tag="KJL94_000656"
                     /pseudo
     CDS             complement(28205..31093)
                     /gene="ptrA"
                     /locus_tag="KJL94_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
     gene            complement(31269..34637)
                     /gene="recC"
                     /locus_tag="KJL94_000657"
     CDS             complement(31269..34637)
                     /gene="recC"
                     /locus_tag="KJL94_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBD4409143.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34650..34982)
                     /locus_tag="KJL94_000658"
     CDS             complement(34650..34982)
                     /locus_tag="KJL94_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBD4409144.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34967..35374)
                     /locus_tag="KJL94_000659"
     CDS             complement(34967..35374)
                     /locus_tag="KJL94_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBD4409145.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35371..35934)
                     /locus_tag="KJL94_000660"
     CDS             complement(35371..35934)
                     /locus_tag="KJL94_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBD4409146.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35925..36395)
                     /locus_tag="KJL94_000661"
                     /pseudo
     CDS             complement(35925..36395)
                     /locus_tag="KJL94_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36579..37373)
                     /gene="thyA"
                     /locus_tag="KJL94_000662"
     CDS             complement(36579..37373)
                     /gene="thyA"
                     /locus_tag="KJL94_000662"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBD4409147.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37380..38255)
                     /gene="lgt"
                     /locus_tag="KJL94_000663"
     CDS             complement(37380..38255)
                     /gene="lgt"
                     /locus_tag="KJL94_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBD4409148.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38406..40652)
                     /gene="ptsP"
                     /locus_tag="KJL94_000664"
     CDS             complement(38406..40652)
                     /gene="ptsP"
                     /locus_tag="KJL94_000664"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD4409149.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40665..41195)
                     /gene="rppH"
                     /locus_tag="KJL94_000665"
     CDS             complement(40665..41195)
                     /gene="rppH"
                     /locus_tag="KJL94_000665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBD4409150.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41880..42569
                     /gene="mutH"
                     /locus_tag="KJL94_000666"
     CDS             41880..42569
                     /gene="mutH"
                     /locus_tag="KJL94_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBD4409151.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42638..43351
                     /locus_tag="KJL94_000667"
     CDS             42638..43351
                     /locus_tag="KJL94_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD4409152.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43489..43707
                     /gene="ygdR"
                     /locus_tag="KJL94_000668"
     CDS             43489..43707
                     /gene="ygdR"
                     /locus_tag="KJL94_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBD4409153.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43815..44855
                     /locus_tag="KJL94_000669"
     CDS             43815..44855
                     /locus_tag="KJL94_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBD4409154.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44888..46078)
                     /gene="lplT"
                     /locus_tag="KJL94_000670"
     CDS             complement(44888..46078)
                     /gene="lplT"
                     /locus_tag="KJL94_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBD4409155.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46071..48230)
                     /gene="aas"
                     /locus_tag="KJL94_000671"
     CDS             complement(46071..48230)
                     /gene="aas"
                     /locus_tag="KJL94_000671"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBD4409156.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48816..49847
                     /gene="galR"
                     /locus_tag="KJL94_000672"
     CDS             48816..49847
                     /gene="galR"
                     /locus_tag="KJL94_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBD4409157.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49854..51116)
                     /gene="lysA"
                     /locus_tag="KJL94_000673"
     CDS             complement(49854..51116)
                     /gene="lysA"
                     /locus_tag="KJL94_000673"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBD4409158.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51238..52173
                     /gene="lysR"
                     /locus_tag="KJL94_000674"
     CDS             51238..52173
                     /gene="lysR"
                     /locus_tag="KJL94_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBD4409159.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52160..52852)
                     /gene="ygeA"
                     /locus_tag="KJL94_000675"
     CDS             complement(52160..52852)
                     /gene="ygeA"
                     /locus_tag="KJL94_000675"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBD4409160.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52981..54399)
                     /gene="araE"
                     /locus_tag="KJL94_000676"
     CDS             complement(52981..54399)
                     /gene="araE"
                     /locus_tag="KJL94_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBD4409161.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54714..55475)
                     /gene="kduD"
                     /locus_tag="KJL94_000677"
     CDS             complement(54714..55475)
                     /gene="kduD"
                     /locus_tag="KJL94_000677"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBD4409162.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55505..56341)
                     /gene="kduI"
                     /locus_tag="KJL94_000678"
     CDS             complement(55505..56341)
                     /gene="kduI"
                     /locus_tag="KJL94_000678"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBD4409163.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56628..57809)
                     /locus_tag="KJL94_000679"
     CDS             complement(56628..57809)
                     /locus_tag="KJL94_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBD4409164.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58064..59293
                     /locus_tag="KJL94_000680"
                     /pseudo
     CDS             58064..59293
                     /locus_tag="KJL94_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14778 a        16059 c        14577 g        14444 t
ORIGIN      
        1 cccccggtga cgtttgttgc cgggttttct cgtttttggt cacttactca tcaactcatt
       61 tcatttgtta tatgaatgtt tcttaccacc ctcacgcgac aaatatcatc acagttaata
      121 tgtcatacaa tttatgttgc aacacaaacg tttccctatt ttcataaacc gtattttatc
      181 gctatgaaaa agaaattatc gccatgatta actaaaagta ttgatttttt cagttcaacc
      241 tacatatatt gcgcgccccg gaagaagtca gatgtcgttt aatgggcaaa tattgccctt
      301 aaattctctt ttacttttga tttacagagt aaagcgttgg gataatctat cttccaagca
      361 gattattgta tttgggatca agatcactga tagatacata acttgtgtgt atctttccgc
      421 cctcaaatta ttacggcggt aaatgattaa gccatcgccg atagacagat ttcattttta
      481 cggtcaggca ccttccctgg ctgaactggc taaaagctga attatttgca ttcctccagg
      541 agaaatagat ggaaacgact caaaccagca cgattgcgtc gaaagactct cgtagtgcct
      601 ggcgcaagac agacaccatg tggatgctgg gcctttacgg cacggcaatc ggcgcgggcg
      661 tgctgttcct gccaatcaac gccggtgttg gcggtatgat cccgctgatc atcatggcta
      721 tccttgcgtt cccgatgacg ttttttgctc accgcggcct gactcgcttc gtactgtctg
      781 gtaaaaaccc gggcgaagac atcaccgagg ttgtagaaga acactttggt attggcgcag
      841 gtaaactgat taccctgctc tacttcttcg ctatctaccc gatcctgctg gtttatagcg
      901 tggcaatcac caataccgtt gaaagcttca tgtctcacca gctgggtatg acgccaccgc
      961 cgcgtgcgat tctgtcgctg atcctgatcg tgggtatgat gaccatcgtt cgctttggtg
     1021 agcagatgat cgttaaagcg atgagtattc tggtattccc gtttgttggc gtactgatgc
     1081 tgctggctct gtacctgatc ccgcagtgga acggcgcagc actggaaacg ctgtctctgg
     1141 acactgcatc tgcaaccgga aacggtctgt ggatgaccct gtggctggca attccggtaa
     1201 tggtgttctc gttcaaccac tctccgatca tctcttcttt cgccgttgcg aagcgtgaag
     1261 agtacggcga tatggcagaa cagaaatgct cgaagatcct ggcattcgca cacatcatga
     1321 tggtgctgac cgtaatgttc ttcgtcttca gctgcgtact gagcctgact ccggcagacc
     1381 tggctgcggc taaagagcag aacatctcga ttctgtctta cctggctaac cactttaacg
     1441 caccggttat cgcgtggatg gctccgatta tcgcgattat cgctatcacc aaatccttcc
     1501 tcggccacta cctgggcgca cgtgaaggct tcaacggtat ggtgattaaa tctctgcgtg
     1561 gtaaaggtaa gtctatcgaa atcaacaagc tgaaccgtat cactgcgctg ttcatgctgg
     1621 taacgacctg gattgttgcc accctgaacc cgagcatcct gggtatgatt gaaaccctgg
     1681 gcggcccaat catcgcgatg atcctgttcc tgatgccgat gtacgcaatt cagaaagtac
     1741 cggcaatgcg taagtacagc ggtcacatca gcaacgtatt cgttgtcgtg atgggtctga
     1801 ttgcaatctc cgcaatcttc tactctctgt tcagctaagt cctttcgcgc cgctttcggg
     1861 cggcgcttcc tccgttttaa cgcgatgtat ttcctatgat tagcgtattc gatattttca
     1921 aaatcggcat tggcccttcc agttctcata ccgttggacc aatgaaagcg ggtaaacaat
     1981 ttaccgacga tctgattgcc cgtaacctgc ttaaagacgt gacccgcgtg gtggttgacg
     2041 tgtacggctc gctctctctg actggtaaag gccaccacac agatatcgcc attattatgg
     2101 gcctggcggg taacctgccg gataccgtgg atatcgattc catccccggt tttattcagg
     2161 atgtgaatac tcatggtcgc ctgatgctgg caaacggtca gcatgaagtg gagttcccgg
     2221 ttgatcagtg catgaacttc catgcagata acctttctct gcatgaaaac ggtatgcgca
     2281 ttaccgcgct ggcgggcgat aaagtcgttt acagccagac ttactactct attggcggtg
     2341 gctttatcgt tgatgaagag cattttggcc agcaggatag cgcaccggtt gaagttcctt
     2401 atccgtacag ttcagcagcc gatctgcaaa aacattgtca ggaaaccggg ctgtcactct
     2461 ctggcctgat gatgaaaaac gagctggcgc tgcacagtaa agaagagctg gaacagcacc
     2521 tggcgaacgt ctgggaagtc atgcgtggcg gtattgagcg cggtatttcc accgaaggcg
     2581 tgttgcctgg caaactgcgc gttccacgcc gtgctgcggc actacgccgg atgctggtca
     2641 gcctggataa aaccaccact gacccgatgg cggttgttga ctggatcaac atgtttgcac
     2701 tggcagtgaa cgaagagaac gctgctggcg gtcgcgtggt gactgcgccg actaacggtg
     2761 cgtgcgggat tatcccggct gtgctggcgt actacgataa gtttatccgc gaagtgaacg
     2821 ctaactcact ggctcgttac ctgctggtag ccagcgccat tggttctctt tataagatga
     2881 acgcgtcgat ttctggtgcc gaagtgggtt gccagggtga agttggcgtg gcgtgctcta
     2941 tggcggcagc tggtctggca gaactgttag gcgcaagccc ggcgcaggtt tgcatcgcag
     3001 cggaaatcgc gatggagcac aacctcggtc tgacttgtga cccggtcgcg gggcaggttc
     3061 aggtaccgtg cattgagcgt aacgccattg cagcagtgaa agcggtgaac gccgcgcgta
     3121 tggcactgcg ccgtaccagc gaaccgcgcg tctgcctcga taaagttatc gaaaccatgt
     3181 acgaaacagg taaagacatg aacgccaagt accgcgaaac ctctcgcggc ggcctggcga
     3241 tgaagatcgt tgcctgcgat taatctctcc cagaggcctc gttttgcgaa gcctcttcct
     3301 gttttctcaa ccagccatag cctgttccgg catcgaatgt tacccttatc gcctgatctt
     3361 taagggggtt atcgtggctg ttcatttgct tattgtcgat gcactgaatc ttatccgtcg
     3421 cattcatgcc gttcagggtt cgccctgtgt cgaaacctgc cagcatgcgc tcgatcagct
     3481 cattatgcac agccagccaa cccacgcggt cgccgttttt gatgatgaaa accgcagtag
     3541 cggctggcgt catcagcgtt taccagatta caaagcggat cgaccgccaa tgccggaaga
     3601 gttgcacgac gagatgcctg cattacgcgc cgcctttgag caacgcggcg tcccgtgctg
     3661 gtcagccagc ggcaacgaag ccgatgactt agccgccaca ctggcggtca aagtgacaca
     3721 ggccgggcat caggcaacga ttgtttcgac agataaaggc tactgtcagt tactttcacc
     3781 gacattacgt attcgtgatt acttccagaa acgttggctg gatgcgccat ttatcgataa
     3841 agaatttggc gttcaaccgc agcagttgcc cgattactgg ggacttgcgg ggatcagcag
     3901 ttcaaaggta ccgggtgttg cgggaatcgg accaaaaagc gccacgcagc tgctggtcga
     3961 gtttcagagt ctggaaggga tatatgagaa tctggatgcg gttgccgaaa agtggcgcaa
     4021 aaaattagaa acccataaag agatggcgtt tctgtgccgc gatattgccc gcttacaaac
     4081 cgatttgcat atcgacggca atttacagca attgcggttg gtacggtaac ggcgagccgg
     4141 atacgccgca aatcgtatcc ggcattatca catcagcgca tttaccaggc ggtatggtaa
     4201 agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca aacatcatcc
     4261 agtgccgcct gagccagtgc cgaaatgtct tccttgcgta caccaacatc acgcaaatgt
     4321 ggcggaatac cgacatcacg gttgagggca aacaccgctt caacagcggc attacgcgcc
     4381 ccttccaggc tcataccttc cactttcacg cccataacgc gcgcgatatc gcggtacttc
     4441 tcaccggtaa agtcagcgtt atagcgcatg acatgcggta acaggatggc gttcgcaaca
     4501 ccgtgtggag tgttataaaa cgcgcccagt ggatgcgcca taccatgcac caaccctaac
     4561 ccaacattcg agaagcccat acccgcaaca tactgcccga gcgccatttc ttctccggca
     4621 tccttatcac cagcaaccga tcctcgcagc gccccagcaa tgatttcaat cgctttaatg
     4681 tgcagtgcat cggttagtgc ccacgcgcca cgggtaatat acccctcaat agcatgagtg
     4741 agcgcatcaa cacccgtcgc ggctttcagc gccggaggca taccatccat catgtcagca
     4801 tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc aaacaaactt gcgccgtttt
     4861 tcttcgtcag tgatcacgta gttaatggtc acttctgccg cagtacctgc tgtggtgggg
     4921 attgccagaa tcggtacact gggtttattg gtcggggaaa gcccttccag gctacgcaca
     4981 tcggcaaact ccgggttgtt gctgataatg ccaattgctt tacaagtatc ctgtggagaa
     5041 ccaccaccaa tagcgatcag gtaatccgcg ccgctattct ggaatacacc gagcccttct
     5101 ttgacgacag taattgttgg gttgggcact acgccgtcgt aaatcgccca tgccagccct
     5161 gcagcatcca tcttatcggt cactttcgcc accacgccgc attgcaccag cgttttatcg
     5221 gtgacgatca gcgccttctg ataaccacgg cgtttcacct catcggttaa agccccaaca
     5281 gcaccccgac caaaccatgc cgtttcgttc agaatcattc tgttagccat catccttctc
     5341 cttgttgctt tacgaaatta ctcttcaatc cgtaacccat aggttttgaa tttctccagc
     5401 actacggcaa tctcttcatc gctcagcact ggcaccgggt ccgtaatcgc cagggtcgtc
     5461 aggtaaagtt gcgccagcac ttcaacttca tgcgccagcc ataacgcttt ttccagattc
     5521 acctcacaag cgataagccc atgatgttgt aacaaagttg ccttacgatt tttgagagcc
     5581 agcgcaacat gttcagaaag ttcgcgtgtt ccaaaggtcg cataaggcgc gcaaggaata
     5641 gaattaccgc cagccgccgc aatcatgtag tgaatagcgg ggatcgatcg gttaagaatg
     5701 gaaactgccg tgcaatgaac ggcatgattg tgaacaaccg cgttggcatc cggtctgctt
     5761 tgataggctg ccatatggaa acgccattcg cttgagggga gctttccttc ctcatgttta
     5821 ccgttgccat caataaagac aatatgcgac tccgtcagtt tttcatatgg aatgcctgta
     5881 ggcgtaatca gcatcccatc ctgataacgt acactgacgt tccccgctgt cccctggttc
     5941 agtcccaggc gggtcatttc caggcaagtg tcaataatct gacgagcaag tttatttcgt
     6001 tccattagct acctctctct gattcaaaac agggcaataa tgttgttcct ttcacactat
     6061 tgaattagcc gtttaattac ccaccatctt cttcctgatt aacaagaaag aaattcacaa
     6121 gcttatattt tgtgacctag ctcaactaat cacagtaaat aactgcaagt tctcttttta
     6181 taaccccgtt aaaaatgacc gctcttaaaa atatttatca aaacggtcat ttttccattt
     6241 cccctcgcca gaaatgcccg ttttcggcac aaacaattaa tacggtcatc tggtttgtgt
     6301 tttttatgat ttattttctg aaacgggcat gaaatttcga ttattaaagt gatggtagtc
     6361 acataaagtc accttccagc taataagtgt gaccgccgtc atattacaga gcgtttttta
     6421 tttgaaaatg aatccatgag ttcatttcag acaggcaaat attcaccgat atgaagcccg
     6481 aactcgctgg ttttgcactt ttgaaaacat aaccgattac gtgcttaagc ttctgaacct
     6541 aagaggatgc tatgggaaac acatcaatac aaacgcagag ttaccgtgcg gtagataaag
     6601 atgcagggca aagcagaagt tacattattc cattcgcgct gctgtgctca ctgttttttc
     6661 tttgggcggt agccaataac cttaacgaca ttttattacc tcaattccag caggctttta
     6721 cgctgacaaa tttccaggct ggcctgatcc aatcggcctt ttactttggt tatttcatta
     6781 tcccaatccc tgctgggata ttgatgaaaa aactcagtta taaagcaggg attattaccg
     6841 gattattttt atatgccttg ggcgctgcat tattctggcc cgctgcagaa ataatgaact
     6901 acaccttatt tttagttggc ctatttatta ttgcagccgg attaggttgt ctggaaactg
     6961 ccgcaaaccc ttttgttacg gtattagggc cggaaagcag tagtcacttc cgcttaaatc
     7021 ttgcgcaaac atttaactcg tttggcgcaa ttatcgcggt tgtctttggg caaagtctta
     7081 ttttgtctaa cgtgccacat caatcgcaag acgttctcga taaaatgtct ccagagcaat
     7141 tgagtgcgta taaacacagc ctggtattat cggtacagac accttatatg atcatcgtgg
     7201 ctatcgtgtt actggtcgcc ctgctgatca tgctgacgaa attcccggca ttgcagagtg
     7261 ataatcacag tgacgccaaa caaggatcgt tctccgcatc gctttctcgt ctggcgcgta
     7321 ttcgtcactg gcgctgggcg gtattagcgc aattctgcta tgtcggcgca caaacggcct
     7381 gctggagcta tttgattcgc tacgctgtag aagaaattcc aggtatgact gcaggctttg
     7441 ccgctaacta tttaaccgga accatggtgt gcttctttat tggtcgtttc accggtacct
     7501 ggctcatcag tcgcttcgca ccacacaaag tcctggccgc ctacgcatta atcgctatgg
     7561 cactgtgcct gatctcagcc ttcgctggcg gtcatgtggg cttaatagcc ctgactttat
     7621 gcagcgcctt tatgtcgatt cagtacccaa caatcttctc gctgggcatt aagaatctcg
     7681 gccaggacac caaatacggt tcgtccttca tcgttatgac cattattggc ggcggtattg
     7741 tcactccggt catgggtttt gtcagtgacg cggcgggcaa catccccact gctgaactga
     7801 tccccgcact ctgcttcgcg gtcatcttta tctttgcccg tttccgttct caaacggcaa
     7861 ctaactgaac atattttccg aataaagtga ggaatctgta atgaaaaaaa tcagcttacc
     7921 gaaaattggt atccgcccgg ttattgacgg tcgtcgcatg ggtgttcgtg agtcgcttga
     7981 agaacaaaca atgaatatgg cgaaagctac ggccgcactg ctgaccgaga aactgcgcca
     8041 tgcctgcgga gctgccgtcg agtgtgtcat ttccgatacc tgtatcgcgg gtatggctga
     8101 agccgctgct tgcgaagaaa aattcagcag ccagaatgta ggcctcacca ttacggtaac
     8161 gccttgctgg tgctatggca gtgaaaccat cgacatggat ccaacccgcc cgaaagccat
     8221 ttggggcttt aacggcactg aacgccccgg cgctgtttac ctggcagcgg ctctggcagc
     8281 tcacagccag aaaggcatcc cggcattctc catttacggt catgacgttc aggatgccga
     8341 tgacacatcg attcctgccg atgttgaaga aaaactgctg cgctttgccc gcgccggttt
     8401 ggccgtcgcc agcatgaaag gtaaaagcta tctgtcgctg ggcggcgttt cgatgggtat
     8461 cgccggttct attgttgatc acaacttctt tgaatcctgg ctgggaatga aagtccaggc
     8521 ggtggatatg accgaactgc gtcgccgtat cgatcagaag atttacgacg aagccgaact
     8581 ggaaatggca ctggcctggg ctgataaaaa cttccgctat gacgaagatg aaaataacaa
     8641 acagtatcaa cgcaatgccg agcaaagccg cgcagttctg cgcgaaagtt tactgatggc
     8701 gatgtgtatc cgcgacatga tgcaaggcaa cagcaaactg gccgatattg gtcgcgtgga
     8761 agaatcactt ggctacaacg ccatcgctgc gggcttccag gggcaacgtc actggaccga
     8821 tcaatatccc aatggtgaca ccgccgaagc gatcctcaac agttcatttg actggaatgg
     8881 cgtgcgcgaa ccctttgtcg tggcgaccga aaacgacagt cttaacggcg tggcaatgct
     8941 aatgggtcac cagctcaccg gcaccgctca ggtatttgcc gatgtgcgta cctactggtc
     9001 accagaagca attgagcgtg taacggggca taaactggac ggactggcag aacacggcat
     9061 catccatttg atcaactcag gttctgctgc gctggacggt tcctgtaaac aacgcgacag
     9121 cgaaggtaac ccgacgatga agccgcactg ggaaatctct cagcaagagg ctgacgcttg
     9181 cctcgccgct accaaatggt gcccggcgat ccacgaatac ttccgtggcg gcggttactc
     9241 ttcccgcttc cttaccgaag gcggcgtccc gttcaccatg actcgtgtca acatcatcaa
     9301 aggcctggga ccggtactgc aaatcgcgga aggctggagc gtggaattgc cgaaggatgt
     9361 gcatgacatc ctcaacaaac gcaccaactc aacctggcca accacctggt ttgcaccgcg
     9421 cctcactggt aaagggccgt ttacggatgt gtattcggta atggcgaact ggggtgccaa
     9481 ccacggagtt ctgaccatcg gccacgttgg cgcagacttt atcacccttg cctccatgct
     9541 gcgtatcccg gtatgtatgc acaacgttga agagaccaaa gtgtatcgtc cttctgcctg
     9601 ggctgcgcac ggcatggata ttgaaggcca ggattaccgc gcttgccaga actacggtcc
     9661 gttgtacaag cgttaataca ttacaccctg atgtgatgcc cggtcgctcc ggctaccggg
     9721 cctgaacaag caagagtggt tagccggata aacaatgtta tccggctata ttgcaggagc
     9781 gattatgaaa caagaagtta tcctggtact cgactgtggc gcgaccaatg tcagggccat
     9841 cgcggttaat cggcagggaa aaattgttgc ccgcgcctca acgcctaatg ccagcgatat
     9901 cgcgatggaa aacaacacct ggcaccagtg gtctttagac tgccattttg caacgctttg
     9961 ctgattgctg tcggcaaatc aatagtgaac tgactgaatg ccacatccgc ggtatcgccg
    10021 tcaccacctt tagtgtggat ggcgctctgg tagataagca aggcaatctg ctctatccga
    10081 tcattagctg gaaatgtccg cgaacagcag cggttatgga caatattgaa cggttaatct
    10141 ccgcacagcg gttgcaggct atttctggcg tcggagcctt tagtttcaat acgttatata
    10201 agttggtgtg gttgaaagaa aatcatccac aactgctgga acgcgcgcac gcctggctct
    10261 tcatttcgtc gctgattaac caccgtttaa ccagcgaatt cactactgat atcacgatgg
    10321 ccggaaccag ccagatgctg gatatccagc aacgcgattt cagtccgcaa attttacaag
    10381 ccaccggtat tccacgccga ctcttccctc gtctggtgga agcgggtgaa cagattggta
    10441 cgctacagaa caacgccgca gcaatgctcg gcttacccgt cggcataccg gtgatttccg
    10501 caggtcacga tacccagttc gccctttttg gcgctggtgc cgaacaaaat gaacccgtgc
    10561 tctcctccgg tacatgggaa attttaatgg ttcgcagcgc ctaggttgat acttcgctgt
    10621 taagtcagta cgccggttcc acctgcgaac tagatagcca ggcatggttg tataacccag
    10681 gtatgcaatg gctggcatcc ggcgtgctgg aatgggtgag aaaactgttc tggacggctg
    10741 aaacaccctg gcaaatgttg attgaagaag ctcgtctgat cgcacctggc gcggatggtg
    10801 tgaaaatgca gtgtgattta ttgtcgtgtc agaacgctgg ctggcaagga gtgacgctta
    10861 ataccacgcg ggggcatttc tatcgcgcgg cgctggaagg gttaactgcg caattacagc
    10921 gcaatctaca gatgctggaa aaaatcgggc actttaaagc ctatgaatta ttgttagtcg
    10981 gtggaggaag tcgcaacaca ttgtggaatc agattaaagc caatatgctt gatattccgg
    11041 taaaagttct cgacgatgcc gaaacgaccg tcgcaggagc tgcgctgttc ggttggtatg
    11101 gcgtagggga atttaacagc ccggaagaag cccgtgcaca gattcattat cagtaccgtt
    11161 atttctaccc gcaaactgaa cctgaattta tagaggaagt gtgaaatgct gaaaacaatt
    11221 tcgccgttaa tttctcccga actattgaaa gtgctggcag agatgggaca tggagatgaa
    11281 attatttttt ccgatgctca ctttcccgcc cattcgatgg aaccgcaggt gatccgcgct
    11341 gatggcctgt tggtgagcga cttgctccag gcgattatcc cgttatttga gctggacagt
    11401 tatgccccgc tgctggtgat gatggcggcg gtagagggtg acactctcga tcctgaagta
    11461 gaacgacgtt accgtaatgc gctttcacta caagccccgt gtcctgacat catccgcatc
    11521 aatcgttttg cgttttatga acgggcgcaa aaagcctttg cgatcgttat cacaggcgaa
    11581 cgagcgaagt acgggaatat tcttttaaaa aaaggggtaa caccgtaatc tcataccggt
    11641 acgcccgcat gacgcgggcg gttatcgaat gatggggtga aaaatatgaa agcggcacgc
    11701 cagcaagcga tagtcgacct gctgctgaac cataccagcc tgaccacgga agctctctct
    11761 gaacagctaa aggtcagtaa agaaaccatt cgtcgcgatc tcaatgaatt acagacgcag
    11821 ggtaaaattc tgcgcaatca tggacgcgct aaatatatcc accgtcaaaa tcaagacagt
    11881 ggcgatccct ttcacatcag gctgaaaagc cattatgcgc ataaagcaga tatcgcgcgc
    11941 gaggcgctcg cgtggattga agaagggatg gtgatagcct tagacgccag ttcaacttgc
    12001 tggtatctgg cacgccagtt gcctgacatc aacattcagg tcttcaccaa tagccatccg
    12061 atttgccatg aactcggtaa acgcgaacgc attcaactga tcagttccgg cggcacactt
    12121 gagcgcaaat atggctgtta cgtcaatccc tcgctgattt cccaacttaa atcgctggaa
    12181 atcgatctgt ttattttttc ttgtgaaggg atcgatagca gcggcgcact gtgggactcc
    12241 aatgcgatca acgctgatta caaatcgatg ctattaaaac gtgccgcgca atcgttgtta
    12301 ttgattgata aaagtaaatt taatcgttca ggggaagccc gcatcgggca tctggatgag
    12361 gtaacgcaca ttatttctga tgagcgccag gttgcaactt ctttggtaac agcctgacgg
    12421 aaagggtagc aggccggaga cgacgccccg gccttgcctg ttatcgctcg tcgcgacgac
    12481 cacccaccgc agcccagatg cggcggacgt gcaccgtcac ttcttcgcga tcgtgataca
    12541 actgccgcgc ctgagtctga gcatttatgc catgttcatc aagctgtgcc tgaatatacg
    12601 ccagattgtg tgatacttct tcgtagcgtt ttttcatcgg cagtttgaga ttgaaaatgg
    12661 tttcacggca ccagccatta accagccact gcgccatcaa tgccgcaact ttagccggtt
    12721 tttcaaccat atcgcatacc atccaggaga tattgctgcg tgtcggacgg aatttgaaac
    12781 cgtcttcccg cagccacgtc acctgcccgg tatccatcag actttgcgcc atcgggccgt
    12841 tgtcgacgga ataaacccac atgttgcgct tcaccagttg gtaggtccag ccgccagggc
    12901 aagcgcctaa atccaccgcc cacatcccgt tcgccaggcg ttcatcccac tcatccgcag
    12961 gaataaacac atgaaatgcc tcttccagtt tgagcgtgga acgactcggc gcctctgccg
    13021 gaaatttcag gcgcggaatg cccatataga acggcgaatt attgttgctg tatgagtaac
    13081 cggtatagca gcatcctggt gcaataaaga atacatgcac aaccggacgc ttcggcgttt
    13141 catagttcgc cagcacccca gcatcgcgca aggcagcgcg tagcggaaca gtgaacttac
    13201 ggcagaattt cagcaactct ttgctttcgt tggtatcggc aacttcaaca cgcagttcac
    13261 cgcccttctc tactacaccc tgtaacatgc cgacaatggg ggtaatacga tcttctggcg
    13321 gcaaatgctg caggagttcc cccaccacaa accactggcg ggcaaaaatt aatgaactga
    13381 acggcagctc acggattaac ttatcgccat catcaggttg ataacattca taaatgacat
    13441 aacccgcatt ctctttcacg cgggcaaaac cgaaaatttc ccgctggccg gctttatcgg
    13501 taatttctgc ggcgcactct ttttcaaagc ccggacggca cagcaataca accttattca
    13561 tgacttacgc ccttgcgctt taaacggata gcaccaacta acattaacgc ccagcccgca
    13621 aggaagctca cgccgccaac cggtgtgaca aacgcccaca aacgcagatg ggacagcgcc
    13681 aggcaataaa ggctgccgct gaacagcacc gtgcctaacg cgaggaaaac gctactccag
    13741 taaaaccaga tactgatgcg acgctgcatt gccaccgcca gacctaagat cgccagcgta
    13801 tgaaacgcct ggtattcgag accggtttgg atccagccca tctcaacggc ccccatggtt
    13861 ttacttaaca catgcgcacc aaaagcgccc agtgccacaa aaatgaagcc gctaatagcg
    13921 gcgaaaatca gcataaaacg gctggtcatg gtcgtaccct acgtaaatta ttgttcataa
    13981 cgaaagcgga atttttcttg ttcagcagcg gctttcgcca ggatccattg gcgaaaggcg
    14041 gctattttac ccagttctgc ctggctgtca tgacaaacca gataaaaagc atttttactg
    14101 accagaacat cattaaacgg gcaaacaaga cgtccggcct cgatttcaga ttgcgccatc
    14161 acgttatttg ccagcgccac tccctgcccg tgaatagccg cttgcagcac catggcgcta
    14221 tgactaaaaa ttggcccttg ctgaacgttg atatgattaa accccaactg tcgggtatat
    14281 gtctgccagt cacggcgtga cgcatcatgt aataacgtat gtttagccag atcttccggg
    14341 gtcttcaagg gtttttcgcc tgtcagcagt agcggcgaac acaccggcaa taaatattcg
    14401 gcgtacagtt tttccacccg tagccccggc cagttgcccc gaccataaaa tatcgccaca
    14461 tcaacatcat ccgccagctt atcttcctga cgatcaaccg cctggattcg aacgtcaatt
    14521 cccggataag ctgaattaaa gctggaaagt cgcggaacca accaatgaat ggcgaaactg
    14581 gggagtaaac tgaccgtcaa cgcccccttg gcgctacggg cctggagttt acgcgtcgct
    14641 tcggttaatt gcgaaaatat ctctttgata tcgaggaaat agctttgccc ttcctcggtc
    14701 agcaggagtg aacgattacg gcggcggaac agttttagcc ccaaaaaatc ctcaagagac
    14761 ttgatttgat gacttactgc ggcctgggtc acaaaaagct cttctgctgc gcgagtaaaa
    14821 cttaaatggc gtgctgcggc atcaaaaact cgtaaggcat ttagcggtgg taatcgttta
    14881 gacatggctt ttaaactttg atgttaaatg aatttaacaa ttagatcaca ctgtgtaacc
    14941 tattagtttt tttaatctga gccattataa attgtccgtt gagcttctac cagcaaatac
    15001 ctatagtggc ggcacttcct gagccggaac gaaaagtttt atcggaatgc gtattctggt
    15061 gaacttttgg cttacggttg tgatgttgtg ttgttgtgtt tgcaattggt ctgcgattca
    15121 gaccatggta gcaaagctac cttttttcac ttcctgtaca tttaccctgt ctgtccatag
    15181 tgattaatgt agcaccgcct aattgcggtg ctttttttta ccttgcgatc gcgaattact
    15241 gttccagttc gaccatctct ttcacgtcag tacggttgat ctgctgtttg ttgccattag
    15301 cgtctttata cgaaatcata ccgatatcgt tatcagtctg tggtttgccg tcagagacga
    15361 tggtacgtcc gtcattggtg tgcatcacat agttcgaacc ggaacaggcg ctcagggcaa
    15421 aagtcagcat acaggcagaa ataattgcgg cagtcttttt catcatcttc tccttaaaac
    15481 gattaataaa tcttaatagt attgattaac aggctaaaat taacgcctaa cactattcag
    15541 catatgttac ttacgcggct tcgccaggat atccagataa ttctgatggt tagcactctc
    15601 cttgtatcaa agtgaatttt gcgtcacgat cggtgcatca agccgaggag taccatgaac
    15661 gtttttaatc ccgcgcagtt tcgcgcccag tttcccgcac tacaggatgc gggcgtctat
    15721 ctcgacagcg ccgcgaccgc gcttaaacct gaagccgtgg ttgaagccac tcgacagttt
    15781 tacagcctga gcgccggaaa cgtccatcgc agccagtttg ccgaagccca acgcctgacc
    15841 gcgcgttatg aagctgcacg agagaaagtg gcgcaattac tgaatgcacc ggatgataaa
    15901 actatcgtct ggacgcgcgg caccactgaa tccatcaaca tggtggcaca atgctatgcg
    15961 cgtccgcgtc tgcaaccggg cgatgagatt attgtcagcg tggcagaaca ccacgctaac
    16021 ctcgtcccct ggctgatggt cgcccaacaa actagagcca aagtggtgaa attgccgctt
    16081 aatgcgcagc gactgccgga tgtcgatttg ttgccagagc tgattactcc ccgtagtcgg
    16141 attctggcgt tgggtcagat gtcgaacgtg actggcggtt gcccggatct ggcgcgagcg
    16201 attacctttg ctcattcagc cgggatggtg gtgatggttg atggtgctca gggggcggtg
    16261 catttccccg cggatgttca gcaactggat atcgatttct atgctttctc tggtcacaaa
    16321 ctgtatggcc cgacgggtat cggcgtgctg tatggcaaat cagaactgct ggaagcgatg
    16381 tcgccctggc tgggtggcgg caaaatgatt cacgaagtga gttttgacgg tttcaccact
    16441 caatctgcgc cgtggaaact ggaagcagga acgccaaatg tcgctggcgt cataggatta
    16501 agcgcggcgc tggaatggct ggcagattac gatatcaacc aggccgaaag ctggagccgt
    16561 agcttagcaa cgcttgcaga agaagcgctg gcgaaacgtc caggctttcg ttcattccgc
    16621 tgccaggatt ccagcctgct ggcctttgat tttgctggcg ttcatcacag cgatatggtg
    16681 acgctactgg cggagtacgg tattgccttg cgggccgggc aacattgcgc tcagccgcta
    16741 ctggcagaat taggcgtaac cggcacactg cgcgcctcat ttgcgccata taatacaaag
    16801 agtgatgtgg atgcgctggt gaatgccgtt gaccgcgcgc tggaattatt ggtggattaa
    16861 tgacaaaccc gcaattcgcc ggacatccgt tcggcacaac cgtaaccgca gaaacgttac
    16921 gcaatacctt cgcaccgttg tcgcaatggg aagataaata tcgccagttg atcatgctgg
    16981 ggaaacagct tccggctttg ccagacgagt taaaagcgca ggctaaagag attgccggat
    17041 gcgaaaaccg cgtctggctg ggatataccg tggctgaaaa cggcaaaatg cacttctttg
    17101 gcaacagcga agggcgcatt gtgcgcggcc tgttggcggt gttgttgact gccgttgagg
    17161 ggaaaaccgc cgccgaatta caggcacagt cgccgctggc attgtttgat gagctgggat
    17221 tacgtgcgca gcttagcgcc tcacgcagcc aggggttaaa tgcgttaagc gaggcgatta
    17281 tcgctgcgac gaagcaggtt taagcacaga accgggtcgg ataagacgtt cgcgtctcat
    17341 ccgacctgat tgtttaaccc tgacgcgccg ctttcgccat catcttcttc agcgcatgag
    17401 aaaccgcaac aaacccaaag gtggcggtca ccatcgttgc cgcgccaaat ccagatgcac
    17461 aatccatccg cttcggccct tctgccgtgg ctttcatcgc acacaccgta ccgtctgact
    17521 gcgggtacac cagcgcttca gtagaaaaca cgcaatccac gccgagctta cctttactgt
    17581 ttttcactac gccaaaatcg cttttcaggc gctcgcgcaa cttcgccgcc agcggatcct
    17641 gaatcgtttt cgccagatcg gtaacctgaa tctgcgtcgg gtcaatctgc ccacccgcgc
    17701 caccggtcgt gaccagcggg attttattgc gccgacaata agcaatcagc gccgctttgg
    17761 gccgtacact atcaatggca tcaatcacgt acgaataacc ggcactcata tattgcgcta
    17821 cgttatccgg cgtcacgaaa tcatccacca ccgttacacg gcactccggg ttaatctggc
    17881 gaatgcgctc cgccatgact tccgcttttg ccagcccgac gttatcgcgc agagcgtgaa
    17941 tttgccgatt ggtattggtg acgcacacat catccatatc gataagcgtg attgcgccaa
    18001 tccccgtgcg cgccagcgct tccgccgccc aggaaccgac accaccgata ccaaccacac
    18061 aaatatgcgc gtcagcaaac agttgcaacg ctttttcacc atacagacgc gctgtgccac
    18121 caaaacgctg acgccatgca tcactaatta ccacagacat agcacctcag aattaaaaag
    18181 ggcgaggtta tccctcaccc taaacgcata aatgcctgat gcgctacgct tatcaggcct
    18241 acataactcc tgcaatattt tggatttgca tgatttgtag gccggataag gcgttcacgc
    18301 cgcatccggt atgaacaacg cgcacttagt caataatcag gatgtgaggt tacccctcac
    18361 cctgtcatat ccgtaaaaac ggcatacaga atatcacatc agccgctaaa gacgttacct
    18421 gcgcccggag ccgttttcag cacccaaacc cgtccatagt ggttgtacca acctgcgcgg
    18481 tgtccggctt ccggcccgat cccttgatag atatcgaagt gttggccttt gattgcgccc
    18541 ccgacatcca acgccaccat cagacgcaat tcgtactgac cgttaaattt gccgttatta
    18601 tccagcagcg gcacttctgc cagcaaggta gtacctggcg gaataattga acgatcggag
    18661 gcaacagaag cacggccaac cagcggcacc gcacttgccc ctttcaccgg tgcaaaagat
    18721 tgcggtttaa agaagacgaa agacgggttc tgttccagca gctcgcgaac ctcggcttca
    18781 ctgtgtgttt cgccccaatg acgaatcgcc tgcatcgaca tatcttcttt tttcacttcg
    18841 ccacggtcga tcagcacctt accaatgctg cgataggcat gaccgttttt ccctgcatag
    18901 ctgaaaaagt taagcggact gccatcacca aagtcgatat acccactacc ctgcacatcc
    18961 ataatgaagt tatccatcag agagttgctg taagcgagga tgtatttatc gcccaatgcc
    19021 ccggcgtaga tctccgcacg agacggcaga cgaccacgtt ttggcggcat acggtaaata
    19081 ggatactgga actcgccctg gcgggtatgg cgcgcctgaa ttaccggcgt gtaatagcct
    19141 gtaaactgca cgttaccata gttgtcggca ccttccatct gccaggcatc aatgccgaac
    19201 tggcgcatat tgcgggtatc accgcctgcg cgcagccact cttgcaccgc gttataaaca
    19261 ttactctggt tgccatacag acgcggtgac gaattacgga tatggttaat ttgctcggca
    19321 aaatcaccgg cgttaatcgg cgcgccaacg gcatctggct ggttcaccag agagaaaggc
    19381 tgggtaaatt tcccgtcttt atattgctgt ccgcgatcgg ttggtttgga agagcaggcg
    19441 gcaagcattg ccacaaccgt gcccataaga aggtacttta cccaacgtcc tttcattgtt
    19501 ctcttcttca ggttaaaaaa taaggcgcaa cgaagataac aaaccgccgc gtttaatgaa
    19561 atgagcagcc tctccctgac gcaaattttg cacaaaaaat aggctttagt ggtttgtttt
    19621 tgttcaaaat catgctaaat ccgtgatcgg ggtaaaaaaa aggttgcatg aaaacgcgag
    19681 cggagtatag tgcgcatcca cggacgcggg gtggagcagc ctggtagctc gtcgggctca
    19741 taacccgaag gtcgtcggtt caaatccggc ccccgcaacc aatcaaattt gatgaagtaa
    19801 aagcagtacg gtgacgcggg gtggagcagc ttggtagctc gtcgggctca taacccgaag
    19861 gtcgtcggtt caaatccggc ccccgcaacc aattattgaa caccctaacg ggtgtttttt
    19921 tgtttctgat ctcccataaa aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc
    19981 cagcgttgcc ccatcggcaa aatacgcttt aatccccgca agaatagact ccgcaacttc
    20041 ctgctggaaa gtcgccgttt tcagtttacg ctcttcctca acgttactga taaacgccgt
    20101 ttcgaccaga atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac
    20161 ttgattttta tgcagcttgt tgatttttcc gagcttattc agtaccgctt taccaaactt
    20221 aaggctgtca gcaatagtca gcgactgcac catatcgaac atggtgtggt cgacatagcg
    20281 gtcaccgctt ttgctcacgc caccaatcaa gtccgaggcg ttctgggttt gtgccagata
    20341 ttttgccgca gtactggttg cgcctttggt tgagagcgca aacaccgagg aaccgctcgg
    20401 ctgacgactg gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg
    20461 ggcttttgct acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac
    20521 cttcatattg ccctcttcct cgatcagaga gcgcaaacgg cgagctattt gcaataccac
    20581 atctttttcg cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg
    20641 gtcaagcata atgacaatcg gacgatcgcg ccctgcttta cccggttgtg gaccactttg
    20701 cgctggcggc acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag
    20761 cgggtcctgc atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa
    20821 cccggcgacc ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac
    20881 catgcgtacg gtttgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc
    20941 tgcacgaatt tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc
    21001 caccaccacg cgttcagggt tactcaacgc gaactgctta tatttcagct gacgatttga
    21061 ttctaccgtc acgcgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct
    21121 aaccgcagcc aggctgacct gacttacgct caatagccac atagcgcccg cgccttgcag
    21181 taaacgacga cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa
    21241 tctaagctgt gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata
    21301 atctgtcatc taataaacgg taaacattct ttttatattc acggcattac tgataaaaaa
    21361 gtcgctctcg cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacaata
    21421 atttcaaata atcatgcaaa ggggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc
    21481 gagggattcc gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc
    21541 atgctcggcg gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg
    21601 ttgttgcaca gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac
    21661 gcaaatctgg ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac
    21721 gccaaaacac tggaactggt gaagcaggct gcgggaacat tgcaactgga tattactgct
    21781 cgcctgtcaa tgagtctcaa taacacgccg ctgcagggcg cgcatatcaa cgtcgttagt
    21841 ggcaatttta ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc
    21901 gggcgtatcc ggcggattga tgaagacgcg ctccatcgtc aactggaaag cggtgcaata
    21961 gtgctaatgg ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa
    22021 gagattgcca ctcaactagc catcaaactg aaagctgaaa agatgattgg tttttgctct
    22081 tcccagggcg ttactaatga cgacggtgat attgtctccg aacttttccc taacgaagcg
    22141 caagcacggg tagaagccca ggaagagaaa ggcgattaca actccggtac ggtgcgcttt
    22201 ttgcgtggcg cagtgaaagc ctgccgcagc ggcgtgcgcc gctgtcattt aatcagttat
    22261 caggaagatg gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt
    22321 gtgatggaaa gcgccgagca gattcgtcgc gcaacaatca acgatattgg cggtattctg
    22381 gagttgattc gcccgctgga gcagcaaggt attctggtgc gccgttctcg cgagcagctg
    22441 gagatggaaa tcgacaaatt caccattatt cagcgcgata acacgactat tgcctgcgcc
    22501 gcgctctatc cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg
    22561 gattaccgca gttcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag
    22621 cagagcggct taagcaaatt gtttgtgctg accacgcgca gtcttcactg gttccaggaa
    22681 cgtggattta ccccggtgga tattgattta ctgcccgaga gcaaaaagca gttgtacaac
    22741 taccagcgta aatccaaagt gttgatggcg gatttagggt aagcgatgaa aatcaccgga
    22801 tgcggcaggc gtaacactcg tgcgtcgcat ccggcaatta cgtttattcc cgcgaactaa
    22861 acaacgccgc cagaccactg cgccgctcag tacgagtggc gattgccgca cttaatatac
    22921 gctcatcggc atacagcgac agacgacgac gcgcgcgggt caccgcggtg tataccagct
    22981 ctcgcgttac taccggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg
    23041 atccctgcga tttatgtacc gtcatcgccc acgtagtttc gtgctctggc aagcgactcg
    23101 gttgcacaga cttaatattg ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccgc
    23161 gatccagcgc aataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa
    23221 tcatcaccgg tcggccttcg taccaacgag agtgcggatg acgatgaatt ttgcgcttct
    23281 gttgcataaa ctgttcaatt cgctcattca gtccagccac gccaaacggc ccttcccgca
    23341 gggcgcacaa aagctggtac tcattgaacg cctgaatgat taaatccggc tcggcacgcg
    23401 cttgcagcag atccagataa cgtccgtaac ccgcaagagc ttcctcaagc atcgcaatat
    23461 aatcttcgcc gctctgtaaa agtcgttttt cgatatcagt aaaatcctgc tgaaaaacgg
    23521 ttttcactgc cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat
    23581 cgctgccgaa acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt
    23641 ctgtgccggt tcctgccgga acgtgagtcc ccgtcaggcg gcttagctgc ctggcacgct
    23701 cggcggtaaa gcccgcgttg gcataagcgc agatatcgcc cagcacagcc ccagcctcaa
    23761 ccgaggccag ttgatcacga tcgccgagaa agatcactcg cgcatgatct ggcaaggcgt
    23821 cgatcagtct cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat
    23881 caagatgcag cgggttaccg gcatgatgac gtaaacgctg gctacccggc tgcgcgccca
    23941 gcaatcggtg caaagtgctg gcatcttccg gaatgcgttt cttttgttca tcggtcagcg
    24001 gtaactgtcg caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg
    24061 gtgcagccag acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagca
    24121 acttcgccac ggtggtcgtt ttaccggtgc ctgggccgcc ggaaatcacc gagatccgcc
    24181 gcgtcagcgc cactgccgct gcaacttttt gccagttaat ttcatcgctt actggaaaaa
    24241 gtttgtccag ggtttgcgcc agtagagctt catcaacctc aatggtatga ttcacctcat
    24301 tgaagaagcg cgctaccgtc cgctcgttac accacatgcg attcaaataa agacgatcgc
    24361 cacagaggat catcggcgtg ggttcatctc ccctgctgac cgcttgagaa gccagcaagc
    24421 attcttccca attttgtagc tcaccgattt cactgacaca ggtcgccaac agcggattcg
    24481 acgcctcgtt attttccagt cgtgaaagcg gcaaacagac gtgtccctct ccggcatcat
    24541 gacttaacag tgccgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg
    24601 caaattgcac atccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt
    24661 gcaatttcat tacgcctcct ccagggtcat accggcaaac atctcatcca tcagggcaat
    24721 caacccggcg ttgggtcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacgcc
    24781 acgcaggaac agatagataa cgccgccaaa gtgaagatca tagtcgtaat cagcaatgcg
    24841 atggcgcaga taacgatgca gcgccagggt ataaagctga tattgcagat catagcggtg
    24901 tgcctgcatc gctgctgcca tagcctgttg ggtgtaagcc gaactgtctt cacccagcca
    24961 gttggattta tagtcgagca ggtaataacg cccttcgtgg cggaatacca ggtcgataaa
    25021 gccttttaac atgccgcgta cctgcatgaa ctccagcggc gggcagccag cggatagcgg
    25081 gtcaaactgg cgaattagtg catcaagctg gctggcgatg agcggttcgc taatcggcag
    25141 gtaaaactcc atctccacct gtttatcgcg gtcggaaagc tgactcaggc taacacccgt
    25201 ttcattgaga ggtgcctgga ggacagccgt gatccactcg gtcaacaccg gttcccactg
    25261 cgattcaaag ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt
    25321 aaaatcgagg tcttcaaaca aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa
    25381 ctgatgcggt gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc
    25441 cagccgtggc atcagatcct gagcgatacc gtgaccacgc tgctgcaagc cggagtagct
    25501 ggtgacacgc cagttatcgc cgggcaatcg ttgtagtgtc ctcgcattca gttctgcagt
    25561 taaagcatca ttaacctgcc agggctggtt atcaccagtt tgtgccgttt gccaggcaat
    25621 atcatcatcg cataacgctt caatacaggt gcgaagccct gccgcatctt gcggttcccc
    25681 tttttgcagc aaacgcccga gcgcactttg gtggacgtcg gtgtcacctt ttttatcgcc
    25741 acgacggcgc accagcggtg caacgccgag actgcaatgc caaaccgaac gcgtcagcgc
    25801 cacgtaaagc aaacgcagat cttccgccag acgttcgacc tccgcgaggt cgacgctttc
    25861 tggcgcagca ttaagatcca aaactgcctc aaacgagtgg cgatcgtgat aaaacgcctg
    25921 atcctggacg cggaaattgg taataaatgg caaccagacc agcggatatt ccagcccttt
    25981 cgatttatgg atcgtgacaa tctgcaccag atgtttatca ctttcgaggc gcatttgttg
    26041 gctggaggca ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg
    26101 ttcactttcc agctgcgttc cggcttcttg aagcagttcg ctgatatgca agatatcggt
    26161 aagacgccgc tcaccgcctg ccgttgccag caagttttct gcaatgttac gcgccgacat
    26221 cagcgcccgc agcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa
    26281 ctcttcgact accgcatccc acgcatgttc gtcattattc agcgtttcaa tatccagcgc
    26341 attcagcccc atcattgacg ttgccagcgc actgcgcagg gtgttctcac gttcgggcgt
    26401 catcaccgcc tgcaacaacc aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc
    26461 gcggttcgaa aggtaaacgg aagggatttc cagcaacgtt aaggcatcgc gcacctgggc
    26521 ggcctcctgg cggctgcgca ccagcacgct gatgtccgaa gcacgcaccg gacgcgcgtc
    26581 gtcgccgttc atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcggatttg
    26641 cgcagcacat acctgcgcca tggtactttg atagtcgcca acgccgcagc tttcgccttc
    26701 catcagccac attttcatcg caggctgcgt ttcaccttta aatacaaaac gtaacgcctg
    26761 atttttcccg gctgatttca ctggaataaa cggtatttcg cgaaacatga acgtgtcatc
    26821 agtctggctg aaaagcttgt tcacactgtt caccattcca ggtgcggagc gccagttggt
    26881 gtctaaagtg tagtgggcgt gaacttcact acgcgccttc atataagtga agatatccgc
    26941 accccggaat gcatatatgg cctgcttcgg gtcgccaatc agcaacaatg cggtttccgg
    27001 ctgatggtgc cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc
    27061 atcgatcatt gccaccggga atcgcgtacg gatcgccgct gccaacacct cgccgctttc
    27121 gctacgcagc gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg
    27181 gcgtttttca cgcgctactg tttcgcggat ctcagccaat gcgcgggtga tcaacagatc
    27241 gcggatcgac aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg
    27301 ggtttccccc ccggccttcg tgcgatcttc taagaaacgc tgggagaatt tttccagcga
    27361 ctccggcaac tgataactgt ttgtctcttc ttctgcccag gcgctgatct tttcgatcca
    27421 tttagcctga ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc
    27481 atccagttca cccactgcgt cgcgccactg ctgttttacc gtatcaatac gcgccacaat
    27541 ttgcgcgtgg cgggaagcca gcgtttcatc atcgggcggc ggtgctttga taaccggcgg
    27601 ttcgccttgc agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc
    27661 aaagacgacc tgggctattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc
    27721 gcaggcctgg tagcgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatgcc
    27781 ggattcaaag gcattcaggt tgagcatgcg ctggcaaaag ccgtgaatag taaagactgc
    27841 cgcttcatcc atctgccgtt cggctaacaa caaccactgc gcggcttgcg ctttatcgtc
    27901 gatctcttcc agcaggcgtt cgtacagtgg attgtcggtg gtttcacgca gacaggcgat
    27961 gcgcaactcg tggatattgc tacggatacg accgcgcaat tctgccgtgg cagcctcggt
    28021 aaaggtgacc accagcagtt cttcaacgat cagcgggcgg ggaaaggcgg cggaaccgcc
    28081 tagtccaagt aacaggcgca aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc
    28141 agaggcttca atcaggcgct cgccctgtaa gggcaagcgc aaaggatcta gtgtctcggc
    28201 gacatcactc attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct
    28261 cccacacttt ccagccttca ggatgcacat attcggcttt cccgttctgg ctgccggaaa
    28321 tctgcgacag aatagccatg ccttgcggct cgaccaccgc ctgatggaag aaatcagcaa
    28381 gtttttgcgg cgtcagcagt tttatctggg ccacgatttt atcacgcgaa tcgaagcgca
    28441 tattgccgcg atcgaaatct ttacttaact tcgatgcttc ttcgccgagc gtttgcggtg
    28501 cctgcagcat ctgggtaatt accgcctgct ggatttgcgc aaactcatct ggcttcatcg
    28561 ctcgcaattt tgcctctgcg gttgggaaaa acgccttgta acgctcccac aagaatgaag
    28621 gctgtttatc attgctttgc aaaaggaagc ccatgcccca ctgacgcccc acgctcattg
    28681 gaaacgcaaa cacggcatag cccaattgtt cttcggtacg caactgattg tagaaccacg
    28741 gctgtacgat ctgccccaac agagagctat aggctgagct ggtgtattca tcgtagccag
    28801 tcggtacaaa taccgctgcc agtgcggagt cggtgctgtt accggctttt tcaaagatga
    28861 cggattgttt tttatcgacc actacatctt tgtttcgaca ccactctgaa ccatcagcgc
    28921 ccaactgttt ttgcacatcg cgtgccagcg ttgttgcctg ggcctcggtc atgttgccga
    28981 taaccataaa ctctggtcga gcccctgatt ttaaggcgtc gcgataggcc agcacctctt
    29041 tcaacgtaat ggagggcaaa attttacgcc gttcatctcg cgagaagtac ggcacttgcg
    29101 agagcatctg cgcgggcata atcgcctgct caaacgcttt acccttttct gcggaatcca
    29161 tcatctggtt ataccaggac ttcgcctgct caagctgctc ttccgtagcg gtatagctaa
    29221 agtacccctc gagcaatgcc tggaacagct gcggcagacg ctgggtgtaa ccattagcat
    29281 taatcataag gccgttgtta gcgttggtgg aaaaacttat gccaccaacc gacgcctagt
    29341 tgcttaactg atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat
    29401 tgcgggcgct gtccatggct ttcggattac gcaaaatcag gctgacatca gctttgggct
    29461 cgctggcaaa ataacggctt ggcgcataca ccacgcgcag attcgactca tcaacaatca
    29521 gctctggatg gtcgtatttc ttctctgact taatcagcga gaaatcatca ggaatataag
    29581 ggttaagctc tggcaaagag agcgcaatgt cggcggcttt tttctgccag tcggcgaaag
    29641 tttgtgcgct gattttatcg acctgatacg gcgcatcgac aaagtaagcc gttttgttgt
    29701 gcggctcttt cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccagac
    29761 gttcctttac tgctttagca tcgtaccgat cggcaatatt gactgcatcc agcgtatgct
    29821 caacaggaac gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga
    29881 tcgacggata acggaagtcg atatccagca cattcgccag ttcatcgaag tattgtttat
    29941 caatgccttt ttcacgtaac agattgagat agctaaaaat tgccgccaca acctgatcgc
    30001 gattagccaa gcctttatca gttaaagacg cagagatcgc taatacgccg ctgttgccgt
    30061 tgacgatagg atcggagttg gcgctaatgc cctcaactaa tccctgcttt tgcagccagt
    30121 cagaaagtgt acctgggctg cgattgccaa tcagataggt aatcaattca tcggttttac
    30181 tacggaactt cgctgagttg ttatcgatgc gaaactcaac gcgcaacact ttacgcggca
    30241 gcgcagggac gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga
    30301 tttccggttt tttgctctct ttgttcggca cgcgaccaaa ggtgtccgcc gccatttttg
    30361 ccaactccgg cagcggttta ttactgtaaa taaccgcctt catcaaattg gcggaatagt
    30421 acttctcgtg gaaatctttc agcgcctgct gcaccggatt accaggtttg tcgcttaaag
    30481 tttcgaggtt accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc
    30541 tgacctgtgc catgcgcatc ccgtcacgcg tacgcgccat ggttaattca gcgttcactg
    30601 cattacgctc acgttcggcg tatttcttgt cgagcaaggg ttctgcaata gcatcggcca
    30661 ggcggtctac cgcaccgggc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat
    30721 acggcgcagt gctggcattg tgactaccgc cgtgcatttt gagatattcg gccagactgt
    30781 cagcctgcgg gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc
    30841 cctggtacgc ctcgggatct tccagcgacc caacgggcac taccagcgcc gagagcgatt
    30901 taactgcctg cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct
    30961 gatactggcg gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg
    31021 tttctgcctg acttaatggt gcccagaggg caacaaacaa caataatgct ttgaaccagg
    31081 tgctgcgggg cattcacgga cctcataatc aactttattt tctggacaga ttcaacaggt
    31141 taacgcaaat catctgccag aatttaatct tgtgctgcac gagtcagcct atgtttatat
    31201 aaccatcagt ccgtgactgg tgcgcatcat aaagtaagcg gatagattgc gcaattttta
    31261 tacaacactc atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca
    31321 acgatggcct ccattgtctc tggtgttaat tgccgccaga gccgttgata ccagatatca
    31381 tcaccttcgc cacgcaccat catgttgcct tcgtaagcct gaaggaattt cgtacgggct
    31441 ttttgcaacg tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt
    31501 agccacgcgc cgccactttc aggtaacacc agcaatggcg cggacattcc ttcacgatac
    31561 ccctcaatca gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc
    31621 cactcaccgt ctttgcgtag aaaaaggcga ctttcgccat taccaccgct ggcacagtag
    31681 acaaggtgtt ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag
    31741 cgcaacaggc catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcag
    31801 gcgagatcaa tttccatact ctgccccggc tggcgacagg caatgactct gtcggcaagc
    31861 tgctgcatct cctggcactg tgtttcccag aaaatttcac caaaagcgcc atacggtaaa
    31921 tcccctgccg cccggaagcg gcggaacaag cgttcggcat catcctgctc aaccagtgca
    31981 ttcaataact gctgattgat ttgataacgg ctaagtcctt ccagaataaa tggctcggtg
    32041 tctgggattt cgctgtcttc agtatgaaag ttcacctgca aacgcatctg gaagaacgcc
    32101 cgcaccggat gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta
    32161 aacggcagcg gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc
    32221 cattcacgag catagctttg tcgttcgcct ggctggtagt tttgcggatc aaacggcatc
    32281 cgggtatgga ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc
    32341 gcttcatcgc ccggtagata atgactttgc ccgatgtagt cgatcagttc ctgcaccagt
    32401 accgacggga aacgttcact gttatcctga atggaacgcc cgatatagct gatatagagt
    32461 ttttgctgcg cggaaattaa cgcttccagg aacaggtagc ggtcgtcatc gcgacggcta
    32521 cggtcgccac gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt
    32581 ggataaacgc cgtcgttcat tcccagcagg caaaccactt tgaacggaat tgaacgcatt
    32641 ggcatcagag tacaaatgtt aaccggtccg gcgagaaaac gctggctgat acgttcttga
    32701 tccagacgct gtgccagttc atcacgcaat agtgacagcg gcaccgcgtc gccatactgc
    32761 gcacctaaac cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct
    32821 tcggtttccg catccggcag gaagaaggcg ttgagcatat cgcgacaaac cggcaaccac
    32881 tcttctagcg gacgctcctg tgccagcccg cgacgccaga tgtttagctg cattagcagt
    32941 gaagccagat gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc
    33001 gattgccact cgccctgcgc gctctccatc gcgtagccca gcaacatacg cgtcaggcca
    33061 aatcgccagg tgtgttgtcc ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct
    33121 atcccccaac gaatgccgga ttcgttgacc cactggcgta aataacgcag cccttcttcg
    33181 gtgatgtcaa accgcgccgc cagcaccggc acatccagca gcgccagcac atcctctgac
    33241 acaaagcgac tgtcaggcag tgataacagg ctgataaacg cttccagtac cggatgtgac
    33301 tgccgcgcac gacggtcgga aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca
    33361 aacactgcct gaataaacgg actgtagctg tcgatatcag ccaccatcac gatgatgtcg
    33421 cgcggagtaa gtgtcggggc ttcctccagc atcgccagca agcgatcgtg taaaacttca
    33481 acttcacgct gcgggctatg gcaaacgtgg aatgtgatac tgctatccag tggatcaagc
    33541 gggcgtttgt tatcgctacg ggaaaactct tcgatgttca cacccgcaac ggcgcggttt
    33601 tccagttcca gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt cacatcgaca
    33661 aaagcgtcca gctcctggct gctctccagg tcagaaagga gataaatgta gtcgcgccca
    33721 agcttccccc atgaggccag cagcgagttg ccgacatcct gttcaccatc gctgttaaag
    33781 agctgtccgg cattttcgct atcgcgaaat agcggcaatt cgcgatcttc aaaactgtgt
    33841 cgacgctgac gagtcagcag tttcgccaga taagcaggat ctttaatatc gccccagtaa
    33901 taacggcagg ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctggagc
    33961 gcctggagat aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtaac
    34021 cccggcgggc aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattcgcg
    34081 cggtgccagc gcggttgtcc gagttcatgg gtatattcca ccagcgcctt ccacaacggg
    34141 gcttgccagg cctgtgcttc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc
    34201 agccagtccg gacgatagac cagatactgg tcaaacaggt ccgccgcttt tgaggaaagc
    34261 tggaacagtt ttcgcttgtc gctatcgtca gtcagataat gccgcaacag ggtaaagtct
    34321 tcgcgctcca gcaattgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta
    34381 aaggcgctct ctttggggat ttccggtaac acccggacga acatatccca gataaagctc
    34441 gctggcagcg gaaaatcaat gtttgccgca ataccaaact tttgcgacag ggtcatttgc
    34501 agccactgcg ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc
    34561 agccgttcgc gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa
    34621 tggtagaccc ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc
    34681 atctcgcctt ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgcc
    34741 tgcgatgtct gcattcggtt gacctgccag ttggcaggtg gcgaaatcgc gcgcagttgc
    34801 gtttgctgcc aaccatgccg ccagagctgt tggtactggt ttctgctggc gagactgttc
    34861 attgatgttc gctggatacc cgataacgca gtgacaatca tcaccatcag caccatcagc
    34921 accatcgcaa ccattacctc cggcaggcta aagccttgtt gattcttcag ggaagctgac
    34981 ataatgcccc ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaacaattt
    35041 ttccatcaat gacttcgcct gttcgccaca acgaaacgcc ttcataaccg gcaatcaata
    35101 aggcttcatt atctgccagt aaacgcaaac aaacctgggc atcggttcca gcgtactgcg
    35161 agcactgaac tgctggctgc gtctgccagg agtgcatttt tccccacgcc agcgccgact
    35221 gaacgatggc ctggcggcgc aatgactgac tttccatgct cacgcgagag gcaaaactgc
    35281 gatcctgctg actcattcct tgcaatagca agctacccaa aaccagcagc atcaggacca
    35341 gagccagtga cgaaacacct ttttcgcggt tcacaggttg aatcctgtca cgctatagct
    35401 ggcatccacc acggtttgcg gttcagactt actggcagca cgcatattaa ccgtcaacac
    35461 cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc
    35521 cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc
    35581 cagcacatgc tccttcagac gaaatccaat ctggtcggac tcttttaccg gttcgcgatc
    35641 ccagataccg ttactgttcg catcccactg cacaatgata cagtcaccct gtccgacaat
    35701 ttccagccct tcgccggtac agctgccatg acaataaccc gccctctgga gatgcttcgc
    35761 gacggtaaat acccgcagcc agatttcatc ttccagtgcc agcttacgag tgctcgttaa
    35821 actttcacgc tgtaacgcag gcagaaagcg tgcagccccc agcaacaata cgctactgat
    35881 cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct
    35941 gtttctcctt gctgataaag ccggagtctt ccccacggcg aaaccaccag ccaccactcg
    36001 cccgttgagt ttttgaagcg aatatgcccg gcccatgcgg tattacgcag gccaaagaaa
    36061 gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac aaagaccaat
    36121 ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca ccataacgtc
    36181 ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa
    36241 tagagcaaat agtcccgcgc ctagctggcg gtttgccaaa gccgttgcga ctgctgccag
    36301 tattgccagc catagagtcc acttgcgctt agcatgacca aaatcagcat cgcgaccagc
    36361 gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa
    36421 aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt acgccgttgc
    36481 aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt aacggagggt aaaaaaaccg
    36541 acgcacactg gcgtcggctc tggcaggatg tttcgtaatt agatagccac cggcgcttta
    36601 acgcccggat gtggatcgta gccttcaatc tcaaagtctt cgaaacggta gtcgaagatg
    36661 gattcgggtt tacgtttgat aatcaacttc ggcagcggac gcggttcgcg gcttaattgc
    36721 agatgagttt gatccatatg gttgctgtac aggtgcgtat cgccaccggt ccagacaaaa
    36781 tcacccactt ccagatcgca ctgctgcgcc atcatatgca ccagtaacgc gtagctggca
    36841 atgttgaacg gcaggccgag gaagacgtca caggagcgct gataaagctg gcaagagagt
    36901 ttgccgtctg ccacatagaa ctggaagaat gcatggcacg gtgccagcgc cattttatcc
    36961 agttcgccta cgttccacgc tgaaacaata atgcggcgcg aatccgggtc gtttttcagc
    37021 tggttcagta ccgtagtaat ctggtcaata tgacgaccat ctggcgttgg ccaggcgcgc
    37081 cactgtttac catacactgg cccgaggtcg ccattttcat cggcccattc gtcccagatg
    37141 gtgacattgt tttcgtgtag ataagcaatg ttagtatcgc cctgcaggaa ccacagcagt
    37201 tcgtggatga tggaacgcag gtggcaacgt ttggttgtca ccagcgggaa tccatcctgc
    37261 aggttaaaac gcatctgatg accaaaaatg gaaagcgttc cggttccggt acggtcgttt
    37321 ttctgtgtgc cttcgtcgag cactttttgc atcagttcta aatactgttt catggttcct
    37381 caggaaacgt gttgctgtgg gctgcgacga tatgcccaga ccatcatgat cacacccgcg
    37441 acaatcatcg ggatggaaag aatttgcccc atgctgatgt actgcaccca ggcaccggta
    37501 aactgcgcgt cgggctggcg gaaaaactca acaatgatgc gaaacgcgcc gtagccaatc
    37561 aggaacaagc ctgagacagc tcccattggg cgcggtttac gaatatacag gttgaggata
    37621 ataaacagca ccacaccttc cagcagcagt tcgtaaagct gtgatgggtg gcgcggcagc
    37681 acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg tttgcagcag caaaatatct
    37741 tctgtacggg agcctgggaa cagcatggca aacgggaagt tcgggtcaac gcggccccac
    37801 aattcaccgt taataaagtt gcccagacgc ccggcaccaa gaccaaacgg aatgagtggt
    37861 gcgataaaat cagagacctg gaagaaggaa cgtttagtac ggcgggcgaa gataatcatc
    37921 accacgataa cgccaatcag gccgccgtgg aaagacatgc cgccgtccca gacacggaac
    37981 agatacagcg gatcggccat aaactgcggg aaattgtaga acagaacata accaatacgt
    38041 cccccgagga agacgccgag gaagcccgca tagagtaagt tttcaacttc atttttggtc
    38101 cagccactgc ccggacgatt cgcccgacgt gttgccagcc acattgcaaa aatgaaaccc
    38161 accagataca tcaggccgta ccagtgaagc gccacgggtc ctattgagaa aatgaccgga
    38221 tcaaactccg gaaaatgcag atagctactg gtcatctgtc accacaagtt cttgttattt
    38281 cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt tcaggcgctc caaaggtgcg
    38341 aataatagca caaggggacc tggctggttg ccggataccg ttaaaagata tgtatatgat
    38401 ccgcgctata accctccgcg aatcagcccg cccatgccgc gacgctccat aaaggctgca
    38461 acctgatgac gaacttcggt cgccagttgc gcttccagac tacgctgcgc aagattttct
    38521 gcttcggcaa aatcaatgcg ccgcagcagg tattttaccc gcgctacaga acgtccgttc
    38581 atagacagat ggcgataccc aagcccaatg aggattgcca cgcacatggg atcgcccgcc
    38641 atttcaccgc acaaacgaag atcgatacca tgtatttccg cttcccgggc gatcatcgcc
    38701 agagctcgta acattgcagg atgaagactg tcgtaaatgt tcgccacccg ggtattgttg
    38761 cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc caacagagat gaaatcgacc
    38821 cgctttgcca gatgcggcag cataaatacc attgacggca cttccagcat gatgccgata
    38881 cgtggtttgg gaatttcgta accgatcatc tcctcgactt cacgtccggc acgttcaatc
    38941 aggcggcgtg cttcgtcaac ttcatcgagg ctggtgacca tcggcaacag aatattcagg
    39001 ttgcccgtag cggcattagc acgcagcatc gcccgcacct ggatcaagaa gatctccggc
    39061 tgatcgagcg taatgcgaat cccacgccaa cccaggcatg gattctcttc gctgatcggc
    39121 atgtaaggca gctgcttatc tgctccgaca tccagcgtac gcaaggtgac gggtttatca
    39181 ttgaacattt gcagcatccc ctgatactgc gccacctgtt cttcttccga cgggaaacca
    39241 ctttgcagca tgaatgggat ttcagtgcga taaagaccga tgccatcaat acagctgccc
    39301 agtttttctt catgttccgg gcttaaacca gcattgagca tgacttttat acgctcaccg
    39361 ctttttaact gcgcgggtaa attgacgtca tcttccgcca gacggctaag ctcaatctct
    39421 tcactaatta gccgctgata ttcttgcagc agtaccggct ccggatcgac cagcaattca
    39481 ccgcgatagc catcaacgat cagcgtccta cgatgcagca ccgaaggctg aatatccgcg
    39541 cccatcacgg tagggatccc cagcgcacgt accatgatcg cagcatggga gttggctgag
    39601 ccatctcgca cgacaacacc gactaagcga tcctggggca gctcagcaag agttgtcgct
    39661 gacagttcat ctgccaccag aatgaaacgt tccggccagg cgttcggccc ttgattagcg
    39721 tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta aatcgccagc ccgctctttg
    39781 agatagttat cgcttagcgc ggcaaactgt tcggcaaatt tttcaatgac cgtttttacc
    39841 gcccactctg ccaccgagcc tttatcaacc tcggcaaaca attcgcgacg cagccgggtg
    39901 tccgaaagca ggtgcgagta aagatcgaaa atagccgccg tttctttttg tgctccggcg
    39961 gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt cttccagcgc cccggtcagt
    40021 cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat acacctgttc cattaaaggt
    40081 aacgtggcat cctgccagcc ttcggcaatc gccacaccag gtgctgccgg taatgcgcgg
    40141 attcgcgtct ggcgatattg cccaaacagg gcagtcaact gcgactgaga aagaatagct
    40201 gccatctggg tggcaagcgt caccaggaag gattcttcac tttcgtcata ctggcgcaac
    40261 tctcgttgct gtaccaccag tacaccaagc aactggcgac gttgaataat tggtacgcct
    40321 aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt atttgaagct ggggtgcttt
    40381 tgcgcatctg caaggtttat cggttccgcc agcctgccaa ccaggccgac gatcccttca
    40441 tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca gcccccgggt cgccatcagg
    40501 tagtaacaac gtcgatcatg atcggccagg tagaccgaac agacctcggt atccatcgca
    40561 agacagatgt cggtaaccag aatatttaac gcctcattca ggcgtggtgc gctggctacc
    40621 ttttcgacta tttcgcgcag gcgagtgagc ataattggcg tgacttaacc tcttttacgt
    40681 cgataagcag atgcgttttg tggttttggc gtattttcct gcagtgacat caccacactc
    40741 gcgaactctt tcattaccct acggtagaca tcacgtttaa atgacaccac ctgtctgacc
    40801 ggataccagt aacttaccca tcgccagccg tcaaactctg gtgtactgct ggtttgcata
    40861 ttgatttctg catcgccgct taccagctgc aagagaaacc atttttgttt ttggccgata
    40921 caaaccggct tcgtgtccca acgcaccaaa cgtttcggta atttgtagcg caaccagtta
    40981 cgcgttgaag caaggattcg aacgtctttg cggcttaatc ctacttcttc aaacaattca
    41041 cggtacatcg cctgctctgc ggattctccg gggttgattc cgccttgcgg aaattgccag
    41101 gagtgctgac caaatcgccg ggcccacatt acctgcccct ggcgattaca aatcacgata
    41161 cctacgtttg ggcggtagcc atcgtcatca atcaccggac tacctcaaaa taaagcttta
    41221 tatacgaatg attgtttcat actccaggaa gacggtaaac cactctctgc agggcattac
    41281 acactaataa caattgaata actcacagtt atgtgcagag ttataaacag aggaaggggt
    41341 ggatagccgt ttttattcac cttttctgtg gatagagttg tgaagaacta cggaattact
    41401 acgggaaaac ccggagcatt ctgaataagc ccgatataga aattttaaaa tattcattat
    41461 tagtcaatgc attacatgta atttgagtaa tgaaaaagag taattcgtga cccaggtcac
    41521 acctctcatt tacgggttgg cgaaagatca accaatgccg tatttatcca cagaatgtgc
    41581 cactaagtta agcactgaac cactaaaaac tggagtttcg tcgcacgtca aggctgtaaa
    41641 tgaaaacagt agtggaggtt tttcacagtt atcccagctt tctgtggata acatggtgta
    41701 agatcctgtt tattttcagt gaccagattt ggaaaaccta ttgcagtgtt gcgcaactcg
    41761 attaccggca gcctaaaaag caatataaat cagagaattg aacaacacat gtggaaaaag
    41821 ttacactgcg aatattcggc acataattac tgtttgtttt ttaaccaagg tatcatgaca
    41881 tgtcccaacc tcgcccactg ctctctcctc ccgaaactga agaacagttg ttagcgcaag
    41941 cacagcaact ttctggttat acattgggag aactggcggc acttgccggg ctggttacgc
    42001 cagagaattt aaaacgcgat aagggctgga ttggcgtgtt actggagatc tggctaggtg
    42061 ccagcgcagg gagtaaacct gagcaagatt ttgctgctct gggcgtggaa cttaaaacta
    42121 tccctgtgga tagtcttggt cgtccgctgg aaacaacatt cgtttgtgtt gccccgttaa
    42181 cgggtaatag cggggtgacc tgggaaacca gccatgtgcg ccacaagctc aaacgcgtac
    42241 tgtggatacc ggttgaaggc gagcgcagca tcccgctggc gcagcgtcgc gtaggatcac
    42301 cgttgctgtg gagcccgaat gaagaggaag accgacagct acgcgaagac tgggaagaat
    42361 taatggatat gattgttctc ggtcaggttg agcggatcac cgctcgtcac ggggaatatt
    42421 tacagataag accgaaagca gcgaatgcga aagcgcttac cgaagccatt ggtgcccggg
    42481 gcgaacggat tctgacgctg ccacgcggct tttatttgaa gaagaatttc accagtgcac
    42541 tactggcccg tcattttctg atccagtagc catcgctttg acctgccgct ttccgggcat
    42601 ataattaccg cttcattttt ttggcagggc tttttagatg ttatttgcat ggataaccga
    42661 tcctaacgct tggcttgcgc tcggtacgct gacgctgctg gagatcgttc ttgggatcga
    42721 caatattatt ttcctttctc tggtggtggc aaagcttccc acagcacaac gtgctcatgc
    42781 gcgccgtctg gggttggcgg gagccatggt tatgcgtctg gcgctgctgg catcaatctc
    42841 ttgggttacg cgcctgacaa atccgctttt tacaatattc agtcaggaaa tttctgcccg
    42901 tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg aaagccagca aggaaatcca
    42961 cgaatccatt gaaggtgaag aagaagggct gaaaacacgc gtttcatcat tcctcggcgc
    43021 tatcgtgcag attatgctgc tggatattat cttcagcctc gactcggtga ttaccgctgt
    43081 gggtctgtca gatcacctgt ttattatgat ggcagccgtg gtgattgccg taggcgtgat
    43141 gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc catccttcgg taaaaatgct
    43201 ggcgctctct ttcctgattc tggtgggctt taccctgatt ctggaaagtt tcgacatcca
    43261 cgtaccgaaa ggttacatct acttcgcgat gttcttctct attgcggttg aaagcctcaa
    43321 cttgattcgc aacaaaaaga atccgctctg atactccgta cgctctcctg cgggagggcg
    43381 tactcactcc ttttaccctc acagtttaag attttcctgc tttcaagata tataacgtcg
    43441 gtttataaac agactattat cataggtagg ccaaatgagg ataaccggat gaaaaaatgg
    43501 gcagtaataa tttctgcagt cggactggcg tttgctgttt ccgggtgttc cagtgattac
    43561 gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg gaaaacctga aattgatgat
    43621 gataccgggc tggtgagtta tcacgatcag caaggtaacg cgatgcaaat taaccgtgat
    43681 gatgtttcgc aaattattga acgttaacaa ataaggtcag catccggctg gccttaagat
    43741 ttttctcttt cccttttccc ttccctctgc catttttata ttccttatgt cgtgattata
    43801 aaaaggaaac ggctatgcaa tatcaccgta taccccacag ttcgctggaa gtcagcacgc
    43861 tggggcttgg cacgatgacg tttggtgaac agaacagcga agccgacgcc cacgcacaac
    43921 tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt tgccgaaatg tacccagtac
    43981 ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt cggcaactgg ctggcgaaac
    44041 atggcagccg cgaaaagtta attatcgcct ccaaagtgag cggaccgtcg cgcaataatg
    44101 acaagggcat ccgcccggat caggcgctgg atcggaagaa tatccgcgaa gcgctgcatg
    44161 acagcctcaa gcgcctacag actgattacc tcgatcttta tcaggtgcac tggccgcagc
    44221 gcccgaccaa ctgcttcggc aaactcggtt atagctggac ggattctgcg cctgcggttt
    44281 cgctgctgga tacgctggac gcactggcag agtaccaacg cgcgggaaaa attcgttata
    44341 tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta cctgcatctg gcggacaaac
    44401 acgatctgcc gcgtattgtc accattcaga acccttacag tctgttaaac cgcagttttg
    44461 aagtaggtct ggcagaagtc agccagtatg aaggggtcga actgctggcc tattcgtgcc
    44521 tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc aaaacccgct ggcgcacgta
    44581 atacgctctt tagtcggttc acccgctata gcggtgagca aacgcaaaaa gccgtcgcgg
    44641 cgtatgttga tatcgccaga cgtcatggcc ttgatcctgc tcagatggcg ctcgcgtttg
    44701 tacgctgtca accgtttgtt gccagcactc tgctgggcgc aaccacgatg gatcagctga
    44761 aaactaacat cgaaagtttg catctggagt taagcgaaga cgtattagct gaaattgaag
    44821 cggtacatca ggtttatact tatcctgcac cataaagatt aacgccggtt aaaaccggcg
    44881 ttaaatatta atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaatagcg
    44941 caccgaagcc aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa
    45001 tgcccagcat caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc
    45061 ccgccccgac gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa
    45121 agccccccag cacgccaatc agcatcaaca aggcataggc tggcagcagc tcgtgttgca
    45181 gggagaaaat cggtaccacc acgccaatca aaatcccggc tggcatacag cgtgacacgg
    45241 tttccagcgt aaccagcttc gctgccgcac ctgcgccaac cacgatacca atcactacca
    45301 tcgcgttgag ataggtcggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca
    45361 gcaccaacag gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg
    45421 aaaaacgcgt ttcaccattg caccatagcg aggtgcaggc attcaggaaa ctgcgggtca
    45481 tgttgatgag attccaggac tgccccggac gcgccgccag tttgggaatg tagatattgg
    45541 caacgaccgc accaccgtag gccagtgcgc atgcggccag agcgaccagg acatgccagt
    45601 cagccagcac accaccggct acggaaccga gtaaaatcgc cgctatggca gaagcttcca
    45661 ttaaaccgtt agcttttact aacttactac ccgtggttaa ttcgccgaga ataccgtatt
    45721 tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga
    45781 taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc
    45841 ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgccaaaaga atgtaagcac
    45901 ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta
    45961 acgtcagagt ggcaaacagt agcgcgttat cgccaaacgc agagagaaac tgcgccacga
    46021 taaccgcttt catccccttc gaccacaacg aagtgttaat gtgcactgac tcactcatcg
    46081 tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca
    46141 agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg
    46201 tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc
    46261 accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc
    46321 gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg
    46381 gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata
    46441 tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact
    46501 tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt
    46561 tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgtgcatc cattcctggt
    46621 agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc
    46681 gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc
    46741 tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag
    46801 tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg
    46861 gtgcaactgc ggtcatacac caagtccggc acaatgcggt aatgcagcgg gcttggataa
    46921 aggaacactt ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag
    46981 tgaaacagcg gtaatgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta
    47041 atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt tggatggcct
    47101 tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc
    47161 aggcgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct
    47221 tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt
    47281 ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata
    47341 gcactggtca gcccttttac cccggcagtg tagttcatca ttgcgggaat gcggcgacgc
    47401 gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg
    47461 cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc
    47521 aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg
    47581 aagcggtaca ttgcactcag taaagattcg tacagagttt cacgcgggcg caccgccata
    47641 cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca
    47701 cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt aatttgcggg
    47761 aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca
    47821 atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag
    47881 attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg
    47941 ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga
    48001 acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa
    48061 acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag
    48121 acgtgattag gcgtaattag tacgcgctcg cccttcagtg cctgggtgtc acccgtaacg
    48181 cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc
    48241 tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg
    48301 acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg
    48361 gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc
    48421 tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt
    48481 gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg
    48541 cgcaggctgg tgtaattcat gtgctcaaca cgaagttgac ttcacctatc aatacctctg
    48601 ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt
    48661 cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct
    48721 cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg
    48781 aaacaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc
    48841 gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca cccaaagcca
    48901 gcgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg
    48961 ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg
    49021 tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg
    49081 gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg
    49141 agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg
    49201 ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc
    49261 ctggctttga aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc
    49321 gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt
    49381 ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattccgg
    49441 ccaatgatcg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga
    49501 ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg
    49561 cggcgggcgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt
    49621 taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc
    49681 gttacccaat cgtaacgatg gcgacccagg ctgccgaact ggctttggca ctggcggata
    49741 atcgccctct cccggaaatt actaatgtat ttagtccgac gctggtacgt cgtcattcag
    49801 tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc
    49861 aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca
    49921 ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg
    49981 ccatatgccc ctgtatcatg cagtaccaga taatcacctg ccttcacttc cggcaaggcg
    50041 cgggtttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc acataacggc
    50101 ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg
    50161 gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccggca
    50221 tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaat
    50281 acgccagact gcgctaccag gaagcgaccc ggttcaattt ccagtttcac agggtggccc
    50341 aaatggcgag cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca
    50401 accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt
    50461 aaatcctgac cgaattcgat aacctgacgc accatagcac cacacacctg ctccagatgg
    50521 gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ctagctgcag atgatgacgt
    50581 tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg
    50641 ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat
    50701 acccgatgcc ctggcgaaac ctggcccagt tggtcgagcg tatcaacaga acccgcattc
    50761 accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta
    50821 aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt
    50881 tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga
    50941 atattggaac aggctttctg tgcaaagcgc accacttcaa actgtttcag cgctgcaatc
    51001 tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa
    51061 cgcagcagat tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca
    51121 aactccagat gagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg
    51181 atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg
    51241 gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg
    51301 actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc
    51361 tttgagaagg tgatcgggct gaaattgttt gagcgcgtac gtggacgatt acatcctacc
    51421 gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt
    51481 gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg
    51541 ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc
    51601 gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc
    51661 cagcgtcatg atttaggact cactgagaca ctccatacgc ctacgggaac agaacgtacc
    51721 gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa
    51781 aaggtattaa cgccggatga ttttcagggt gagaattaca tcagcctttc ccgtactgac
    51841 agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc
    51901 gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtatttcg
    51961 gtggttaacc cgctcaccgc actagattat gcggcaagcg gtttagtggt gcggcggttc
    52021 agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg
    52081 ctggttcagg cgtttagtgg gcatttacaa gcggggttac cgaaactggt cacttctctt
    52141 gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg
    52201 ccgcggtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg
    52261 tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat
    52321 aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat
    52381 taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca
    52441 gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag
    52501 tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag
    52561 tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac
    52621 cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat
    52681 cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca
    52741 tttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt
    52801 aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct
    52861 cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca
    52921 ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa
    52981 tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag
    53041 cgtgacattt ttggtttccg gaatgagcca gaaagtaata cccacaaacg caatgttcag
    53101 cgcagtgtag agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggc
    53161 cgcgccgata atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc
    53221 gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat
    53281 cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga
    53341 agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat
    53401 cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc
    53461 aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc
    53521 tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg tagtacatga tgatgttcat
    53581 accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt
    53641 acggttgatc ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc
    53701 gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc
    53761 ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag
    53821 aataatcagc agaactgctg gtaaagcaag accccccaac attgcgcgcc agttaccgct
    53881 ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg
    53941 gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg
    54001 agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat
    54061 ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc
    54121 catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat
    54181 tgctgcgccg agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa
    54241 gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa
    54301 tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat cacgcaaaga
    54361 acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt
    54421 aagacattgt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa
    54481 atatggatta aattgctgcg acatgccgtt atgtgatgga tattccaatt ttaaaattaa
    54541 gttgaattat tgagattatt attaacctcc taattttaaa gcagataaaa ttcataaagt
    54601 tcattaattg ataattgata tggattattt cataaccatg atatggatta tgatgatcta
    54661 gagatataaa aaaccctgcc atgcggcagg gtcataaaag taagaggaat gaattaacgc
    54721 gccagccaac cgccatccac agcaatggca taaccattta cataatctga agcgctggag
    54781 gcaagaaaca ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg
    54841 cgatcgagaa tttccgcgct acgttgttca tctgcccgca gttgttgggt attgttggtc
    54901 gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgtttgcc
    54961 atcaatcgcg tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc
    55021 ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg
    55081 ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg
    55141 acatcgtccc agtctttttc gctgaactcg agagcgtctt cgcggcgaat caatccggcg
    55201 ttattcacca ggatatcaat atgaccaaat tcagctaccg cgcgatccag cagtccagga
    55261 atgccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc ctgcgccgtg
    55321 acctgctcga tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct
    55381 tgcgccagcc ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca
    55441 actttacctt ggagagaaaa tgcacttaaa atcataacaa caccttattc gtttatgccc
    55501 acaactagcg caaatcttta acgcccacat ggtccatatc attaaagacc tggttttcac
    55561 cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatgg atcgaccagc
    55621 tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc
    55681 ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc
    55741 ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca
    55801 gccccatact caattgacag gtttccagta catccgggac aaaatatttg ttaatcgtgc
    55861 gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt
    55921 ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc gccggagtgg
    55981 cggtatcaat actggcaaag acaacttctt ttgcaccttt accaacatac agggcgtcgc
    56041 ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt accggcaccg ccaatattga
    56101 taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc
    56161 cgccaacgga aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt
    56221 ggctgtaaac catggtgtac tcatcggcga caaatacctt ttcaaccaaa aattcattgc
    56281 gcagcccttg ggtatccagc gtttttgcgt gcgcaccgtg gatgctctgt cttacgtcca
    56341 catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc
    56401 gttttctcta taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca
    56461 atgttttatt tttaatttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg
    56521 agaatggaaa aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca
    56581 acagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt
    56641 tcaatggtca atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg
    56701 gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct
    56761 atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca
    56821 agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg
    56881 acatcagcca actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca
    56941 attcccatca atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg
    57001 ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg
    57061 tttatggatg atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa
    57121 cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac
    57181 gtctgaccgt tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca
    57241 atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg
    57301 atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca
    57361 tcccacaacc catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg
    57421 gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg
    57481 acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagaccg
    57541 gaaccgcaaa cgtcattaat cgtgattgca gaaacgctgt taggaagacc acctttaata
    57601 gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact
    57661 tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta
    57721 ccaagttcca cggcggaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt
    57781 aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag
    57841 taaatgattg ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag
    57901 gaaatatgcc tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac
    57961 ccaccaatag atgggtagat agggcgttaa tctcccatac ttaacttggt ttatggtaaa
    58021 ttgccctcca ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc
    58081 taaagaagaa actctgtgaa gtttcgcgct ctatggcaca gccgttgatg caggcacgct
    58141 attccttcct attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt
    58201 cgcctggcct ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcaaa
    58261 aacatcagca ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg
    58321 taatctgatt accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt
    58381 ggcaattacc aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat
    58441 ccgtatgttg gtgagtctgg gtgttgtatt aattctgaat ctcatttttc tgatgggacg
    58501 tcatgccact attcgggtaa tgggattttt ggtattccca ttgattgcct atttcttatt
    58561 tctttccatt taccttgtcg gtagttggca acctgatcta ttaacaaccc aggtagagtt
    58621 caatcagaat acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag
    58681 ccatacgccc attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc
    58741 tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact
    58801 gttctttgtc tttagctgcc tgctttctat tccatcttcg tatattgaag cggctaaaga
    58861 agaaggggtc accattttat cggcgctttc tatgctgccg aacgccccag catggttgtc
    58921 aatttccggg attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt
    58981 tggcgttatt gaaggtgcca cagaggtcgt caaaacaaca ctacaacagg ttggtgtaaa
    59041 gaaaagtcgt gcatttaacc acgcactatc aattatgttg gtatcgctga ttaccttcat
    59101 tgtttgttgc attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat
    59161 tgccatgata cttttcatca tgcctacgct gtcaacgtat ctcatcccgg cgcttaaacc
    59221 ctggcgttcc atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat
    59281 gttctttagc taatatccgt tgtggatggt tcaaaattgc tacaattttg aaccattcca
    59341 tttttcataa tattcatttg ttattattcc gccctatcac ttctctcgtc atatcctaga
    59401 attatcagtt agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat
    59461 gcataactta gattcgcaag atatcttctg gtcattcaaa aacaatttgc gatatagata
    59521 ttctcatacc atgagatagt tatctggacc acgttcgacc atgcaagatt aaatcagtga
    59581 aacataatca tatttaataa cgagaaatgc atttttaaat atcttaatca gaattatagc
    59641 aaatacagat taatcgcaat ttttcccagc aaaattacaa aatcaaggtg tattgacatc
    59701 atcaatcacc aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct
    59761 ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc
    59821 agaagagtca ccgcgtttgc agtactgcgc aaactcgt
//
LOCUS       DAEBXD010000011        58519 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEBXD010000011 DAEBXD010000000
VERSION     DAEBXD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58519
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.011"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..1142)
                     /locus_tag="KJL94_000681"
                     /pseudo
     CDS             complement(1..1142)
                     /locus_tag="KJL94_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(1354..1701)
                     /gene="pspE"
                     /locus_tag="KJL94_000682"
     CDS             complement(1354..1701)
                     /gene="pspE"
                     /locus_tag="KJL94_000682"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBD4409165.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1744..1965)
                     /gene="pspD"
                     /locus_tag="KJL94_000683"
     CDS             complement(1744..1965)
                     /gene="pspD"
                     /locus_tag="KJL94_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBD4409166.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1974..2333)
                     /gene="pspC"
                     /locus_tag="KJL94_000684"
     CDS             complement(1974..2333)
                     /gene="pspC"
                     /locus_tag="KJL94_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBD4409167.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2333..2557)
                     /gene="pspB"
                     /locus_tag="KJL94_000685"
     CDS             complement(2333..2557)
                     /gene="pspB"
                     /locus_tag="KJL94_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBD4409168.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2611..3279)
                     /gene="pspA"
                     /locus_tag="KJL94_000686"
     CDS             complement(2611..3279)
                     /gene="pspA"
                     /locus_tag="KJL94_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBD4409169.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLRETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3446..4423
                     /gene="pspF"
                     /locus_tag="KJL94_000687"
     CDS             3446..4423
                     /gene="pspF"
                     /locus_tag="KJL94_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBD4409170.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4543..5808)
                     /gene="puuE"
                     /locus_tag="KJL94_000688"
     CDS             complement(4543..5808)
                     /gene="puuE"
                     /locus_tag="KJL94_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBD4409171.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5846..7126)
                     /gene="puuB"
                     /locus_tag="KJL94_000689"
     CDS             complement(5846..7126)
                     /gene="puuB"
                     /locus_tag="KJL94_000689"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBD4409172.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7128..8615)
                     /gene="puuC"
                     /locus_tag="KJL94_000690"
     CDS             complement(7128..8615)
                     /gene="puuC"
                     /locus_tag="KJL94_000690"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBD4409173.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8765..9322)
                     /gene="puuR"
                     /locus_tag="KJL94_000691"
     CDS             complement(8765..9322)
                     /gene="puuR"
                     /locus_tag="KJL94_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBD4409174.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9349..10113)
                     /gene="puuD"
                     /locus_tag="KJL94_000692"
     CDS             complement(9349..10113)
                     /gene="puuD"
                     /locus_tag="KJL94_000692"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBD4409175.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10325..11743
                     /gene="puuA"
                     /locus_tag="KJL94_000693"
     CDS             10325..11743
                     /gene="puuA"
                     /locus_tag="KJL94_000693"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBD4409176.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11892..12056
                     /locus_tag="KJL94_000694"
     CDS             11892..12056
                     /locus_tag="KJL94_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409177.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12046..13353
                     /gene="puuP"
                     /locus_tag="KJL94_000695"
                     /pseudo
     CDS             12046..13353
                     /gene="puuP"
                     /locus_tag="KJL94_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
     gene            13468..13713
                     /locus_tag="KJL94_000696"
     CDS             13468..13713
                     /locus_tag="KJL94_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBD4409178.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14026..15669
                     /gene="sapA"
                     /locus_tag="KJL94_000697"
     CDS             14026..15669
                     /gene="sapA"
                     /locus_tag="KJL94_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBD4409179.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15666..16631
                     /gene="sapB"
                     /locus_tag="KJL94_000698"
     CDS             15666..16631
                     /gene="sapB"
                     /locus_tag="KJL94_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBD4409180.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16618..17508
                     /gene="sapC"
                     /locus_tag="KJL94_000699"
     CDS             16618..17508
                     /gene="sapC"
                     /locus_tag="KJL94_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBD4409181.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17508..18500
                     /gene="sapD"
                     /locus_tag="KJL94_000700"
     CDS             17508..18500
                     /gene="sapD"
                     /locus_tag="KJL94_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBD4409182.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18502..19308
                     /gene="sapF"
                     /locus_tag="KJL94_000701"
     CDS             18502..19308
                     /gene="sapF"
                     /locus_tag="KJL94_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBD4409183.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19376..19729
                     /locus_tag="KJL94_000702"
     CDS             19376..19729
                     /locus_tag="KJL94_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBD4409184.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20097..20885
                     /gene="fabI"
                     /locus_tag="KJL94_000703"
     CDS             20097..20885
                     /gene="fabI"
                     /locus_tag="KJL94_000703"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBD4409185.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21029..22156
                     /locus_tag="KJL94_000704"
     CDS             21029..22156
                     /locus_tag="KJL94_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBD4409186.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22224..24158
                     /gene="rnb"
                     /locus_tag="KJL94_000705"
     CDS             22224..24158
                     /gene="rnb"
                     /locus_tag="KJL94_000705"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBD4409187.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24236..24338)
                     /locus_tag="KJL94_000706"
                     /pseudo
     CDS             complement(24236..24338)
                     /locus_tag="KJL94_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24392..26366
                     /gene="pdeR"
                     /locus_tag="KJL94_000707"
                     /pseudo
     CDS             24392..26366
                     /gene="pdeR"
                     /locus_tag="KJL94_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26514..26687
                     /locus_tag="KJL94_000708"
     CDS             26514..26687
                     /locus_tag="KJL94_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409188.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26777..27526
                     /locus_tag="KJL94_000709"
     CDS             26777..27526
                     /locus_tag="KJL94_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4409189.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27795..28013
                     /gene="osmB"
                     /locus_tag="KJL94_000710"
     CDS             27795..28013
                     /gene="osmB"
                     /locus_tag="KJL94_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBD4409190.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28139..28465)
                     /gene="yciH"
                     /locus_tag="KJL94_000711"
     CDS             complement(28139..28465)
                     /gene="yciH"
                     /locus_tag="KJL94_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBD4409191.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28465..29202)
                     /gene="pyrF"
                     /locus_tag="KJL94_000712"
     CDS             complement(28465..29202)
                     /gene="pyrF"
                     /locus_tag="KJL94_000712"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBD4409192.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29395..30564)
                     /gene="lapB"
                     /locus_tag="KJL94_000713"
     CDS             complement(29395..30564)
                     /gene="lapB"
                     /locus_tag="KJL94_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBD4409193.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30571..30879)
                     /gene="lapA"
                     /locus_tag="KJL94_000714"
     CDS             complement(30571..30879)
                     /gene="lapA"
                     /locus_tag="KJL94_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBD4409194.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31028..31792)
                     /gene="pgpB"
                     /locus_tag="KJL94_000715"
     CDS             complement(31028..31792)
                     /gene="pgpB"
                     /locus_tag="KJL94_000715"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBD4409195.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31962..32552
                     /gene="ribA"
                     /locus_tag="KJL94_000716"
     CDS             31962..32552
                     /gene="ribA"
                     /locus_tag="KJL94_000716"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBD4409196.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32616..35291)
                     /gene="acnA"
                     /locus_tag="KJL94_000717"
     CDS             complement(32616..35291)
                     /gene="acnA"
                     /locus_tag="KJL94_000717"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBD4409197.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35455..35550)
                     /locus_tag="KJL94_000718"
     CDS             complement(35455..35550)
                     /locus_tag="KJL94_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409198.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35664..35831)
                     /locus_tag="KJL94_000719"
     CDS             complement(35664..35831)
                     /locus_tag="KJL94_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409199.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35834..35998)
                     /locus_tag="KJL94_000720"
     CDS             complement(35834..35998)
                     /locus_tag="KJL94_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBD4409200.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36293..37267)
                     /gene="cysB"
                     /locus_tag="KJL94_000721"
     CDS             complement(36293..37267)
                     /gene="cysB"
                     /locus_tag="KJL94_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBD4409201.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37477..40074)
                     /gene="topA"
                     /locus_tag="KJL94_000722"
     CDS             complement(37477..40074)
                     /gene="topA"
                     /locus_tag="KJL94_000722"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBD4409202.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40454..40705
                     /locus_tag="KJL94_000723"
     CDS             40454..40705
                     /locus_tag="KJL94_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBD4409203.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40741..41790)
                     /gene="sohB"
                     /locus_tag="KJL94_000724"
     CDS             complement(40741..41790)
                     /gene="sohB"
                     /locus_tag="KJL94_000724"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBD4409204.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42010..42768
                     /locus_tag="KJL94_000725"
     CDS             42010..42768
                     /locus_tag="KJL94_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBD4409205.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42765..43355
                     /gene="cobO"
                     /locus_tag="KJL94_000726"
     CDS             42765..43355
                     /gene="cobO"
                     /locus_tag="KJL94_000726"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBD4409206.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43395..44267)
                     /gene="rluB"
                     /locus_tag="KJL94_000727"
     CDS             complement(43395..44267)
                     /gene="rluB"
                     /locus_tag="KJL94_000727"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBD4409207.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44368..44988)
                     /locus_tag="KJL94_000728"
     CDS             complement(44368..44988)
                     /locus_tag="KJL94_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBD4409208.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44985..45866)
                     /gene="yciV"
                     /locus_tag="KJL94_000729"
     CDS             complement(44985..45866)
                     /gene="yciV"
                     /locus_tag="KJL94_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBD4409209.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46004..46048
                     /gene="trpL"
                     /locus_tag="KJL94_000730"
     CDS             46004..46048
                     /gene="trpL"
                     /locus_tag="KJL94_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBD4409210.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46025..46118
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46140..47702
                     /gene="trpE"
                     /locus_tag="KJL94_000731"
     CDS             46140..47702
                     /gene="trpE"
                     /locus_tag="KJL94_000731"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBD4409211.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47702..49297
                     /gene="trpD"
                     /locus_tag="KJL94_000732"
     CDS             47702..49297
                     /gene="trpD"
                     /locus_tag="KJL94_000732"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBD4409212.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49298..50659
                     /gene="trpCF"
                     /locus_tag="KJL94_000733"
     CDS             49298..50659
                     /gene="trpCF"
                     /locus_tag="KJL94_000733"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBD4409213.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50671..51864
                     /gene="trpB"
                     /locus_tag="KJL94_000734"
     CDS             50671..51864
                     /gene="trpB"
                     /locus_tag="KJL94_000734"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBD4409214.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51864..52670
                     /gene="trpA"
                     /locus_tag="KJL94_000735"
     CDS             51864..52670
                     /gene="trpA"
                     /locus_tag="KJL94_000735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBD4409215.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53051..53230
                     /locus_tag="KJL94_000736"
     CDS             53051..53230
                     /locus_tag="KJL94_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBD4409216.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53316..53816
                     /locus_tag="KJL94_000737"
     CDS             53316..53816
                     /locus_tag="KJL94_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD4409217.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53862..54368
                     /locus_tag="KJL94_000738"
     CDS             53862..54368
                     /locus_tag="KJL94_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD4409218.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54428..55066)
                     /gene="ompW"
                     /locus_tag="KJL94_000739"
     CDS             complement(54428..55066)
                     /gene="ompW"
                     /locus_tag="KJL94_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBD4409219.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55306..55395
                     /locus_tag="KJL94_000740"
                     /pseudo
     CDS             55306..55395
                     /locus_tag="KJL94_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55423..56166
                     /locus_tag="KJL94_000741"
     CDS             55423..56166
                     /locus_tag="KJL94_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBD4409220.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56196..56735
                     /locus_tag="KJL94_000742"
     CDS             56196..56735
                     /locus_tag="KJL94_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBD4409221.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56840..57238
                     /gene="yciA"
                     /locus_tag="KJL94_000743"
     CDS             56840..57238
                     /gene="yciA"
                     /locus_tag="KJL94_000743"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBD4409222.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57278..58006)
                     /gene="tonB"
                     /locus_tag="KJL94_000744"
     CDS             complement(57278..58006)
                     /gene="tonB"
                     /locus_tag="KJL94_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBD4409223.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58221..58517
                     /locus_tag="KJL94_000745"
     CDS             58221..58517
                     /locus_tag="KJL94_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBD4409224.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14050 a        15133 c        14624 g        14712 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga catatggttg cagacaaaat caaacattaa atgactgcat
      781 tcaccgagtt gctgaatatc ctgccactcc cccgcttcac tggcgacctg atgataatca
      841 attaccgaaa atccatcatc agatgaccat gggtaaaaag gtaataaatg aacatgtgaa
      901 aaaatgcttt gcagccattg atggtagaac tgattaaata tgggtagtgg ttttaaatca
      961 ttactgtgaa attgatcggc ataggtaatt aacacgacat cactttcatc ccagtgtttt
     1021 ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac gcgttacaag tttctgtaaa
     1081 tgagttgcgg taaatgttcc accgtagatt tcgtccatgt aatccgtaat tttctgtttc
     1141 atgttgcctt tccgtggtag cggtcccatg aggcgagaga ctactccgca gaaaataaac
     1201 tgtcaattga gcacaagggt tttagtcacg caggaaatca ttttttacca gggaaaaagc
     1261 gtatttgtga gctgacgcaa ataatctata gggatgtaaa taaggtgtga cagaaaaaaa
     1321 acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg gcattgcgat
     1381 gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa ggagctcttt
     1441 tgcctgcccg gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
     1501 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ccccctgaac
     1561 gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
     1621 gggcagtgaa aacaccagtg ccagagcaag taagcctttt tttaaacatg taagcgtcct
     1681 tctatggatg aaaattgcca tccattatac gcctgagatt taacaatttt tgcgaaactc
     1741 ccctcacctt ttataacgct gtgccagttt attagcagcc cgacttaaca gcggttccag
     1801 cgccacagcc agcaacattt tcagcggtcg gcgagcaact gattttatcg cccagcccgc
     1861 cacacccgcc gggccatagc gcagtgcggt aagaagtacc agcttgcctg ctaatttgaa
     1921 accaagcttc accttttgcc cggcctgttg ccagcgagta ttcataactt tcctcacagt
     1981 tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga cataacgttc catctcgcgt
     2041 aaacgcgttt cacttgccgc cagttcacgg tcgacttcat ccagcaattc gctgctggaa
     2101 ggtagctgct caccaaaggc catgttgtcc ggcattggat caagcgcaaa tgacaaaatg
     2161 atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa tcgacagcac caccaggata
     2221 cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc cggcgcagac gccgcggacc
     2281 atgccctgct gtggaatacg ccataacttt ttattgagat taatgcccgc cattagcgat
     2341 ccctccagtt cggatgttct gcatcaagaa tagattccag cgcctgaata cgttcgcgca
     2401 tccgttttgc ttcatcaacc agttgcgcta atcgctgctg ctcactttgc gacaattcac
     2461 tgcgaccaga acgattgctg taatgcagcc ataaccagat cggtaaaaca aacagcacaa
     2521 aaatggttaa cggaatagcc agaaatagcg cgctcataag tactccttag acgatgagcg
     2581 gcggcgcgtt cggacgccgc ctggatgtta ttattgattg tcttgcttca ttttggcttt
     2641 taattgtgcc agttgttcgc tgattgcatc atcggctttc agttcggcaa actgatcgtc
     2701 cagcgatttt tgtttaccga agctgtggct ttctgcttcc gcttccatct ggtcaatacg
     2761 acgttcgaaa gattcgaaac gagccattgc ttcatccagt ttgccactgt ccagctgacg
     2821 acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac atcaatgcct gctggcgagc
     2881 gcgtgtttct ctcaatttgt tttccagctc gccaatctct ttcttcatgc gtgccagcgt
     2941 atcgtccacc agcgtcactt catgttccag ggacttaatc agatcggtca gtttctgttt
     3001 ttcaattaac gctgcacgtg ccagatcctc tctctctttc agcagcgcca gttcggcttt
     3061 ttcctgccat tcaacttcac gcgccgacgc ttgttcaata cggcgagtca gctgtttctt
     3121 ttctgccaac gcacgcgccg aagtagaacg tacttcaacc agtgtatctt ccatctcctg
     3181 gatcatcaga cgcaccagtt tctgtggatc ttccgctttc tctaacagag cgttgatgtt
     3241 ggcattcacg atgtcggcaa agcgagaaaa aatacccata atgttgtcct cttgatttct
     3301 gcgttcagga ttgtcctgct gaactgttaa tacaatttgc gtgccaattt tttatctttt
     3361 tgatttataa agatctgatt gaagaatcaa cagcaacatg ccaggatgaa ttagcgaatt
     3421 acactaacaa gtggcgaatt tcatcatggc cgaatacaaa gataatttac ttggtgaggc
     3481 gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc gcaccgctgg acaaaccggt
     3541 gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt gccagccgcc tgcattatct
     3601 ctcctcccgt tggcaagggc cgtttatttc ccttaactgc gcggcgttaa atgaaaatct
     3661 gctggattcc gaactgtttg gtcacgaagc gggggcgttt accggtgcgc aaaaacgtca
     3721 tcctgggaga tttgaacgtg ccgacggcgg tacgctattt cttgatgaac tcgctacggc
     3781 accgatgatg gtgcaggaaa aattattgcg tgtgattgag tacggtgaac tggagcgcgt
     3841 tggcggtagc cagccgttgc aggtgaatgt gcggttggta tgcgcgacga atgccgatct
     3901 cccggcgatg gtcaatgaag gtacttttcg cgctgacctg ctcgaccggc tggctttcga
     3961 tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac attatgctga tggcagaaca
     4021 ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg ttcccggggt ttacggagcg
     4081 cgccagagaa acattgctga attatcgttg gccgggaaat attcgtgaat tgaaaaacgt
     4141 ggtggaacgt tcagtgtatc gccacggcac cagcgattat ccgcttgatg acatcattat
     4201 tgatcccttt aaacggcgtc cacctgaaga agccatcgcc gtttcagaaa atacctcgct
     4261 tccgacgctg ccgctggatt tacgcgagtt tcagatgcag caggaaaaag agttgctgca
     4321 actcagtttg caacagggga aatataacca gaagcgcgcg gctgaattac tggggttaac
     4381 ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt tagcgaagtt gagcaataca
     4441 acttgccgga tgcggcgtga atgctttatt cggcatacat aatcaatacg tcgtttgcag
     4501 gcctgataag cgcagcgcat cagacattat tgcgttgggc tattaatcgc tcagcgcatc
     4561 ctgcaaaatt ttcattgccg catcgaattg cgcatccggg atggtcagcg gatacaggaa
     4621 gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc agcccctgcg ccagcgcgcg
     4681 ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc gtttgcggat cgttaaactc
     4741 tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg gccggaacgc tttctttggc
     4801 atcaatcaac gtgtttttga gacgctggcc cagttgattc gcgcgttcgc agagtgattc
     4861 tttgtcgata atattgagca ccgcgtgcgc ggcagccacc gccagcgggt tcccggcgta
     4921 ggtgccaccc aacccgcccg gcgcgggcgc gtccataata ttcgcgttac cgaccacgcc
     4981 cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc attaagtccg gcttatcggc
     5041 gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag ccgctttgca cttcatcggc
     5101 aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg gcggcaacca gctcttttgg
     5161 cgcaacgttg aaaccgcact cgccctgcac cggttcgaaa ataatggccg cgacctgctt
     5221 cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg agggagtcct gtgttgaaat
     5281 gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa ccagggaacg ggccgaagcc
     5341 gattttgtac ggcgcaactt ttccggtcag cgccatggtc atatatgtac gaccgtggaa
     5401 gccaccgcta aacgcaatca cgccaggtcg tccggtatgg gcgcgagcaa ttttcaccgc
     5461 gttttccacc gcttccgcgc cggtagtgaa gaatgcggtt ttagcctgcc cgctcactgg
     5521 ggcaagggtg ttaattttct ccgccagcgt gacgtagctt tcgtacggca caatctgata
     5581 cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc gcgaccagat cagggtggcg
     5641 atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg atgtattcgt tgccctctac
     5701 gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag ttacacatca cgccaacccc
     5761 gcgtggggta gcagaaagac gacgctgatg gaattcattg ttgctcatgt ttcacacctt
     5821 ttgactcttc actgttaatc ggaaattaaa cgcccagacg atcgcgcagg ctgtaataag
     5881 ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc gccggggaac gggtaatgcg
     5941 gcagattggc gaaggcatcg aaacgctcgg cgtcaccgcg cagcagttca gcaatcaaac
     6001 gcccggcaag atgggtacag gtgacgccgt ggccgctgta accctgcatg taatagatgt
     6061 tggtatcaag gcggccgaac tgcggcatgt gcgacaaggt cagcaggaag ttgcccgtcc
     6121 agcggtaatc aattttcacg cccttcaact gagggaaggt ttttagtagt ttcggtacca
     6181 caagacgctc aacgtcatcc gggttgcgtg caccatacac tacacccccg ccgtacagca
     6241 ggcggttgtc ggcggtaaga cggtagtaat caagcagata gttacagtct tccacacagt
     6301 agtttttcgg gatcagcgaa cgggctaaat cttccgacag ccgttcggtg gtgatcacct
     6361 gcgtgccgca cggcatgctg cgtttcgcta gttccggctc tactttatcg cccagatacg
     6421 cattcccggc gacaatcaca tacttcgccg tcacctgacc attggcagtc cgaaccacgg
     6481 ctggcgtggt gtgctggatc tgcgtcacgg cggaaagctc atacacgcgc ccgccgttca
     6541 ggcggatggc atcggcttca ccaatggcga ggtttagcgg gtgaatatgc ccaccgctgt
     6601 gatccagcag cgcaccagtg tagcgatcgc tggcgacctc gcggcgaata gcgttgacgt
     6661 ccagcagctc cagctgttta ttgccgtagc gttcccagtt ctctttctgc tcttcgagtg
     6721 tggcgagttg tttatcattc atcgccacaa acagaccgcc ggggcggtag tcgcagtcaa
     6781 tctgataacg tttgatacgt tcgcggatga tctcgccacc ctcgaacatc atgctgccga
     6841 gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac gtcgatgtcg cggctgtagg
     6901 agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa gccgatgcgt gaggcttcga
     6961 gaaccactac gtcaaagccc gcttccgcca gatgcagcgc ggaggagagc ccggtatagc
     7021 cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt cagtgtgtcg aatggtgcat
     7081 atttattcgc actggcggcg tagtaactgc tggtatgttc ggtcatttca ggcctccagg
     7141 cttatccaga tggttttcag ttcagtgaat ttttcaaggg catgcaggga tttgtcgcga
     7201 ccgttgccgc tctgcttata gccgccaaac ggcacggtca tatcgccgtc gttgtagtta
     7261 ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc ggtgcgcgcg ggagaggtcg
     7321 cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt tggcaagctg cagcgcctga
     7381 tcttctgatg tgaaacgggt gaccaccagc accggaccga aaatctcttc gcggcttaag
     7441 gacgcattcg ggtccacatc cacgaagatg gtcgggccga tggccgctgc cagcccggcg
     7501 ttgcgaccat ccagcagcag ttgccccttg ccttcgcctt cacgaataaa ggtgtgtacc
     7561 gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg ttgcgggatc aagtggatgg
     7621 cccggctgcc agttttgcgc ctgctgtttt aacagggcta agaattcatc ggcgatgctc
     7681 tcttccagta acaaacgcgt tccggcgatg cacacctggc cctggttgta gaagatgcct
     7741 gcggcggtgg cgcttgccgc tttttgcaga tccggacaat cggcgaaaac gatgttggcg
     7801 cttttgccgc ccgcttccag ccagacacgt ttcatgttgc tatcacccgc gtctttcagc
     7861 agttgtttac ccgtacgcgt ggagccggta aaggcgatgg catcgatatc gttatgacgc
     7921 gaaagcgcct gccccgcttc atgaccaaaa cccgtcacca cgttcaacac accatccggc
     7981 aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac tgagcggtga tttttcagac
     8041 ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc cgagtttcca gcaagtcagc
     8101 aacagcggga agttccacgg cacgatggcg gcaatcacgc cgaccggttc acgcacgatc
     8161 atcgccagct catggctact ggtggtcgcc acttcgccat acactttgtc gatcgcttcg
     8221 gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat cacgcagact gtgacgaatc
     8281 ggtttgccgg tgtcgagagt ttccagcagt gccagctctt cggcgtgggc ttccattaaa
     8341 tcggcgagtt tattcagtac cgctttacgt ttagccggag aagagagtga ccagtcgccg
     8401 cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga tatcgacgct cttgccgcgg
     8461 gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg tttcaaaggt ttcattttcc
     8521 gccgcagcag tatattcacc gttaataaat aagcggtttt caatggcgag acttaacgct
     8581 ttatcctgcc agtaagccag atgatgaaaa ttcattatga ctcctgtttc acgtctatca
     8641 gatatatgca gtttgtgaac gtcagggcgc agagatcccc gggctgcatg cccgtttatt
     8701 tctggcgaca ttctttatgg attgccgggc gcattgcgct tacccggctt acagaacaaa
     8761 aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat
     8821 tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgaggtggt
     8881 aatcctgacc attaatcgtc agaacaattt caccttccag tactgtgcct atttcctcac
     8941 cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg
     9001 ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat tgacacaccc tgactgccca
     9061 tctcaattaa gtcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg
     9121 aaaagaattc cgagagtgac aaaccataca ccttcagcag cttttgcagc gtactgatgg
     9181 cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt
     9241 cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg
     9301 gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg
     9361 tttttcagcg atatggtgct gccaagcggt gataaagccc tcgaacaata tacgcgaaag
     9421 cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt
     9481 gatgacgcta accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg
     9541 gctaacgacc ttcgcccctt gtccatgtag agagtttacc caaaagttgc tacattcagg
     9601 taacaacgca gacagtaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg
     9661 ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag
     9721 cttgcgatgc aacgaaccac cggttgccac caccagttct tgtaaacccc ggcagatggc
     9781 gaaaatgggg atgcgccttt cgagtgcggc attaattatc gccatgctca gaagatcacg
     9841 cccgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct
     9901 gggactacta ggaagataaa tgccatcgag tttcggcaaa agttgttcaa gtaacgacgg
     9961 ttccgccagc gcatgtggta gcgcaattgg caagccgcct gcgtggatga tggcattcag
    10021 gtacttttct tgcagagtct gggtcgcatg acccttaagc ctgttcctgc acattacgac
    10081 accgataacc ggattgttca ttatattttc catgctcaat ctcacaaagt ggactaaatt
    10141 atcgccatta ctgcgcttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg
    10201 attttcattt ttgcaaactc aatttaacat ttgacaaaca tttggtttgc atacagattc
    10261 gaatgatggt cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag
    10321 aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg
    10381 gggtagcacc tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata
    10441 tgttgatgtt ttacttactg atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc
    10501 aagcctgaag aagctcgaga aagggtgtta tttcccggcc tcggtatttg caatggatat
    10561 tctgggcaac gtagtagaag aagcaggtct gggtcaggaa atgggcgagc cggatcgtac
    10621 ctgtgttcct gttctcggtt ccttaactcc ttctgccgcc gatccagagt ttatcggtca
    10681 gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt
    10741 tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg ttcccggttg tagcggtaga
    10801 gctggagttc tatttactgg atcgccagcg cgacgctgaa ggggatctgc aaccgccctg
    10861 cgcgcctggc accgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa
    10921 ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttgcagc tgattccggc
    10981 agatggcgcg gtcgctgagg cctcgccggg tcagtttgaa atcaaccttt accatactga
    11041 taacgtgctg gaagcctgcg atgatgcgct ggcactaaaa cgcctagtgc gtctgatggc
    11101 agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag
    11161 cgggatgcat atccatatca gtatgcaaaa taatcgtggc gagaatgtgc tttctgacac
    11221 agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc
    11281 gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccgggaat
    11341 gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc accgtcgccc tgcgtattcc
    11401 gtgcggtgac cgtcataatc accgcgtgga atatcacgtg gcgggtgccg atgccaaccc
    11461 atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc
    11521 gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct tcccgattcg
    11581 ccagagcgat gccctgggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga
    11641 acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct
    11701 catcacagaa acagaaattg agtggatgtt gaaaaacgcg taattcccct tgcggggttg
    11761 tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga
    11821 tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta acgattaaga ctgtgacgag
    11881 gaaatgagat gatgccgatg attaaatccc cgcatggcga gggaggctgc gtatgcgccc
    11941 ctcctgctac tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca
    12001 ggtcgacacg accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca
    12061 ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg
    12121 caggtggtaa tgatgggtct ggcctatctc acgccgatga ccgtatttga tacttttggt
    12181 attgtctccg gcattagcga cggtcacgtt ccggcgtcct atttgctggc gctggcgggc
    12241 gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg
    12301 gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg gatttatggt cggctggtca
    12361 tcactgctgg attatctctt tttgccaatg atcaacgttt tgttggcgaa aatctatctc
    12421 tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta
    12481 accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg
    12541 gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga
    12601 gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc
    12661 ccgattatta ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc
    12721 acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg
    12781 gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttctttccc
    12841 gatatcagcc gctttaaaga cccggacgcc gcactgcctg aaattgcgct gtacgtcggc
    12901 ggcaagctct ttcagtcgat tttcctctgc accacgtttg tgaacacatt agcgtctggc
    12961 ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt
    13021 ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga ctccggcact caacgtcatt
    13081 atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt tagtcaccgc gacagcactg
    13141 attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc
    13201 tggcggcgta aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg
    13261 ctggttggcg cgctgacggt gggtgtgctg tgggttaacc tagagtcaac gtcactgaca
    13321 ctcggtctgg tatgggcttc gttaggcggc gcattatcct gcctgagggt aacgcgccgc
    13381 gccattaact ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc
    13441 ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat
    13501 attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg
    13561 ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa
    13621 gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc
    13681 gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt
    13741 atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg
    13801 tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt
    13861 tatagttata gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa
    13921 cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat
    13981 taaatattag taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta
    14041 tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc
    14101 cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac
    14161 acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt
    14221 tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgcctga acttgccgaa
    14281 agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg
    14341 tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt
    14401 accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc
    14461 ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat
    14521 cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc
    14581 cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag
    14641 gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc
    14701 gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg
    14761 caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg
    14821 gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg
    14881 cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc
    14941 aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag
    15001 cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc
    15061 gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc
    15121 gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt
    15181 tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg
    15241 caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg
    15301 atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac
    15361 agtttcttcc gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac
    15421 tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca
    15481 gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg
    15541 ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt
    15601 agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa
    15661 aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc
    15721 tgaccttcgt tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt
    15781 cactgtggaa tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt
    15841 ccagcattaa cggtcaacca attgccgaac agttaaaaga gctcttcccg gcgacgatag
    15901 aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg
    15961 ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct
    16021 tttcaatccc ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag
    16081 gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt
    16141 ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg
    16201 caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga
    16261 tccgcctgat gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg
    16321 cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac
    16381 cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca
    16441 ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc
    16501 aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta
    16561 acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg
    16621 ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc
    16681 aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta
    16741 ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat
    16801 caattattgc cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac
    16861 gacctcggac gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc
    16921 gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg
    16981 gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc
    17041 ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct
    17101 atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg
    17161 catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg
    17221 aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc
    17281 cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca
    17341 caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc
    17401 gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac
    17461 ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg
    17521 atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc
    17581 gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg
    17641 gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg
    17701 ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca
    17761 aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt
    17821 cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt
    17881 ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga
    17941 ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc
    18001 agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac
    18061 cgacaaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca
    18121 accaaaacag caataccact attttgctta tcagccatga cttacaaatg cttagccaat
    18181 gggcggataa aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg
    18241 agttggtgac gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact
    18301 tcggcagcgc gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc
    18361 tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat
    18421 gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc
    18481 tgaacatgga gaaaaagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa
    18541 acgttccgct accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg
    18601 agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa
    18661 tccacactgg caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt
    18721 gacgatcatc cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt
    18781 tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt
    18841 ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg
    18901 atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc
    18961 ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt
    19021 gccgatgaag ccctcgcttc actggatatg tcaatgcgtt cgcagttgat taatctgatg
    19081 ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg
    19141 atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc
    19201 agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg
    19261 cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc
    19321 tccttccaga ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat
    19381 ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa
    19441 gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc
    19501 ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga
    19561 tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc
    19621 gcagggctgg accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt
    19681 gctggagatt attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg
    19741 ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc
    19801 ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt
    19861 ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt
    19921 tagggtaagg ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta
    19981 tagctactca cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa
    20041 acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg
    20101 gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct
    20161 acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg
    20221 acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc
    20281 agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt
    20341 ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg
    20401 gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct
    20461 acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc
    20521 tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg
    20581 caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg
    20641 tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag
    20701 acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt acagttacta
    20761 ttgaagatgt gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg
    20821 gtgaagtagt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga
    20881 aataatcgtt ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc
    20941 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    21001 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    21061 ctggtatcat gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga
    21121 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc
    21181 ccccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca
    21241 gcttaccgtg acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac
    21301 ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc
    21361 gctccccgga cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
    21421 tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa
    21481 tgacgtcggt cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg
    21541 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt
    21601 acccgggctg gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg
    21661 ctggcgaagc agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc
    21721 agaactttgc caactggccg aaacacactc tctccaccct atttcacttt cccttctcga
    21781 aaagttgtta aacagcacac gggtcaatgc acagccggat aatcagcttg cggcgttgtt
    21841 atgcgcgcgt ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga
    21901 atataaaaaa atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga
    21961 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
    22021 tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat
    22081 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    22141 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    22201 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    22261 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
    22321 ttggcttcct ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa
    22381 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    22441 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
    22501 aaaatgaccg tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc
    22561 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
    22621 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    22681 ttggtgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag
    22741 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
    22801 cctcgctgga ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt
    22861 tcgctaaggc gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg
    22921 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    22981 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    23041 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca
    23101 ccactgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    23161 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa
    23221 ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata
    23281 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    23341 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    23401 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
    23461 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
    23521 cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc
    23581 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    23641 catttgctga aattagctct gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    23701 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    23761 cggttatcaa aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg
    23821 aacgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
    23881 tcctgaaaga taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc
    23941 gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt
    24001 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
    24061 aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca
    24121 tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct
    24181 catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac
    24241 tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa
    24301 acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc
    24361 ggactccgct gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt
    24421 gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt
    24481 gcgcttttct accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca
    24541 ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt
    24601 cgatgaaagc gatctttctg tgcatctggt aggacgaaaa atcaataaac gggaatgggc
    24661 tggcaacgcg tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct
    24721 ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg
    24781 ggggaatatc caacgcttca atcggttatg tgaagattac acagggttga aagaacacga
    24841 cgtcattggg taaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag
    24901 gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc
    24961 aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg
    25021 caaaaacgag atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca
    25081 ggagcgactg cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc
    25141 aatgcaggag ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt
    25201 gtatcttgat ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga
    25261 ccagttatta cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt
    25321 ggcgcgtcca ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct
    25381 ggaagcaatg gcatcacgaa ttttgacccg cttacgcctc ccctttcgta ttggtttaat
    25441 tgaagtttat accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag
    25501 cgcgactatt attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg
    25561 acaattttgc gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga
    25621 taccaacttg cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg
    25681 gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc
    25741 caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt
    25801 gggtgattct cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc
    25861 gagtggcggt aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga
    25921 aacaggttct ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga
    25981 gttgtctgat tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag
    26041 gtgcgcaagt gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc
    26101 gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac
    26161 ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg
    26221 tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca
    26281 atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt
    26341 ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg
    26401 tcattatata tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta
    26461 gcggcaattt ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg
    26521 aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag
    26581 gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg
    26641 ttgctgaatc tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc
    26701 aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt
    26761 tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca
    26821 gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg
    26881 ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt
    26941 ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga
    27001 actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg
    27061 cagcagcaat gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat
    27121 cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct
    27181 cggtggcgtg taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat
    27241 ccagcaggta catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg
    27301 attcaccggt cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg
    27361 cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg
    27421 tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat
    27481 gcatcttgag cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc
    27541 tacggatgat ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta
    27601 agactattta cctgttatta acactctcaa gatataaaat tattatcagc gatataacag
    27661 gaagtaatta tcacctgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt
    27721 caccaggctt attcttagct attatagtta tagagagctt acttccgtga atcataaatt
    27781 caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta
    27841 ctttggcaat gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa
    27901 tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta
    27961 cattaggtgg tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa
    28021 taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt acgaaataac
    28081 tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc tttttatgtt
    28141 aaccgcctgc gagttttacc ttcatccctt tcgcttcaag tagtgatttt aataaatcac
    28201 gcttatcgcc ctggatttca ataactccat ctttaactgc tccgccgcag ccgcattttt
    28261 tcttcagttc cgctgcaagt tttgtcagtt cggcatcatc gagatcgata ccggtaatca
    28321 ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc gctccatcgc
    28381 ctttagggcg tactggggcc gctttgggct catcaatacg cccggtttcc gttgagtaga
    28441 ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt tgatcgcttt
    28501 cagtgtctgc gctggatcta ccgattgcgt taccgggcga ccaatcacca tataatcaac
    28561 accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcaccag cttcactccc
    28621 ctgcggacga atgcccggtg taaccagttt gaactcctga ccgaatacct gtttaaagcg
    28681 cacagcttcc tgagcagaac acaccacacc atcaaggtca catttttgcg tcagtgctgc
    28741 cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca ggtcgctggc
    28801 ttccatgctg gtcaacactg tcacagcaat caaaagcggt gcatctttgc caaacggaac
    28861 cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg gaggcatgaa cattcaccat
    28921 ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg ggatatcgtg
    28981 gaatttcagg tcaagaaaga tatcaaaacc acgctgttga agttcgcgca caaactgtgg
    29041 cccaaacaat gtaaacatct ctttaccgac cttcagacga caatcgcgtg ggtcgatctt
    29101 gtcgacaaag gacagcgcgt catcacaatt atgataatca agggcaacaa ccacaggaga
    29161 attcgtaacg gcgcgggaag aagatgaagc agttaacgtc atgaccagac cttcttgatg
    29221 atgggcaccg ggagtggcgc gaaacaggta aacggcgagc attctacccg tgaaaataaa
    29281 aaattaacag ttgcgatttt tcccctagca ggctttttct gctccgtcgt tgccggagca
    29341 caatgggaca tcaataacta gtatgttgta actaaagtcg gattttttta aaaattacag
    29401 gccatcaaga ccgcgaatcg gtttaatagt tgaccaggca cgacaagacg gacaatgcca
    29461 gtagagggtg tatgcagtaa aaccacattt ctggcagcta taacgaggct tactacgcac
    29521 cttctcgcca accatgtcac gcagcaccat cagactctct ttggcgcgcc cttcttccgc
    29581 ttcatttaag tggtaatcca ttagcttatg gaatacacgc atggtcggat gacgctgaag
    29641 ctggcgtgta atatagacct gtgcagcctc actaccgtcg cgcgcttcga tgatatcagc
    29701 aagcatcaat tcagcatcgg caccggtgtt ctcttccacc gcgcgttgca ggaattctgc
    29761 ccattcggca gttttaccca actgctgata gcaggtttgc aacatttcca gcgtttcgct
    29821 gaccagttct ctgtcctggg atatgacgcg ttgcaaactt tcgacggctt tggcgtattc
    29881 tcctttcgcc ataaacacgc gtcccatcat gatggatacg cgggcgctgt ttttatctgc
    29941 cgccgcccct ttttttagca aggtcatggc acgatcgaga tcgtcgctgg ccatatgctg
    30001 cagggctaac tcacagtaga aatgggcaat ttcgacgcgc tgtttatctt tacccagctt
    30061 caccaggcgt tcggcaacat caattgcttt ctgccactca ctggtagcct ggtagatttg
    30121 tagcaactgt tgcagcgcgc caatgcggaa gtcagtttca tcggtcagct gattgaacat
    30181 gtcttccgcg cggtcgtata acccggcggc catgtaatca cgccccagtt gttgaatcgc
    30241 caacagacgc tgttcatagg tcagcgaggc gctttccatt agggtctgat ggatgcgaat
    30301 agcgcgatca acttcgccac gcgaacggaa caggtttccg agcgtaaggt gggcttcaac
    30361 ggtgcctgta tcctctttaa gcatatcgag aaacaggtct accgctttat cctgttgatt
    30421 actaagcagg aagttaaccc ccgctacgta atcacgcgac aagcggttgg cttcatcttg
    30481 cttgttttgt tgcgcacttc tgcggcccat ataccagcca taggcagcgg ctacaggcaa
    30541 aagcagaaac aacaactcca gcatagaaag ttattccttc gccgctgacg agtgcggcac
    30601 tacagccacg tcagtcgcgg gtgaaagctg gttttccagt cgctttattt tacgttcagc
    30661 gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca caaatcaacc aaccgatagc
    30721 aaaccccgca gcaaacaata ccgccagcaa tgtggaaata cggtactccc cttgcgctaa
    30781 cagataatta aacgtcacct gttgatcgtt ctgcgcaccc aacgtgaccg aaatcacgaa
    30841 gatcgctaac accagtaaga aaatgagtaa atatttcaca ttacttcccg ttatgtggtt
    30901 agagcgaata aattatggtc aacttaccgc aatagccctt taaagtacca ttttcgcgcc
    30961 gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa ggcctaaatt cggaaaatca
    31021 accagcgtta actttcttgt tctcgttgtg caatttcgcg attttcttcc gcaggtggtg
    31081 ttaatggccc acaaattcgt tgcgcaagcc acgttgccac cgccaccagc gcccacgaaa
    31141 tcaacgtagc taccaccaga tcgcgtggcc aatgcatccc gagcagcagg cggcttccca
    31201 tgactcccgt tgcccagacc aacaagatag caatggttaa cgttcgccga cgcggccaca
    31261 gcaaaccaac ggccagcagt gcccaactgg cagcaaacat cgtgtgaccg gaaggaaagg
    31321 caaaccccgt ctctttctgc cagtgtgaac gcaaatattg tggaatattt ttctcttcag
    31381 ccaactgttc ttttactaga tttccgcgtt ctgctcgctt taaagtgtag aactcatcaa
    31441 ccggaatatg atgtgttttt tccaaccaga taacaaaagg tcgtggttcc tgtactttgt
    31501 ctttgatcca ggatttaacg ccttgtccaa caaggattgc cgccgccaga atggcaaata
    31561 atacaaaggc agccttaatg cgaaaacgca gacaccagag aaaccagccg aacaaaatca
    31621 aatgtgtaat gacgccccag ggctgggtga cagtttcagt aacccaaaaa gccgctttta
    31681 gtagccaact ttgttctcca ggttgccaac gccagccaga aatccatacg gctactggca
    31741 tgacaagcaa tagtgcagct cccactgcgg tacgtcgggc aatcgaacgc atggcctctc
    31801 ctttttgata agtcccacaa tcataactga aaatgccagt tccaagaaaa attgacagat
    31861 ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt gaaccccttc tcgttatggc
    31921 aaaataagcc aatacagaac cagcattatc tggagaattt catgcagctt aaacgtgtgg
    31981 cagaagccaa actgccaacc ccatggggcg atttcctgat ggtgggattt gaagaactgg
    32041 caaccggaca cgatcatgtc gcgctagtct atggcgatat ttccgggcat accccggtac
    32101 ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct gttcagcttg cgctgcgatt
    32161 gtggcttcca gctcgaagcg gcattgacgc aaattgccga ggaaggccgt ggtattttgc
    32221 tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa taaaatccgc gcttacgcac
    32281 tgcaggatca aggttacgat accgtagagg ctaaccacca gttaggcttc gccgctgatg
    32341 agcgcgactt cactctttgc gctgatatgt tcaaactcct tggcgtcaat gaagtccgct
    32401 tgttaaccaa taacccgaaa aaagtcgaaa ttctgaccga agcagggatt aatattgttg
    32461 aacgcgtacc attgattgta ggtcgtaacc ccaataacga acattatctc gataccaaag
    32521 ccgagaaaat gggccatttg ctgaacaaat aaccctcttg catcgtgtaa ttcatttgct
    32581 tgccggaagc aaaataaccg gcaagcaaat acttgttact tcaacatatt acgaatgaca
    32641 taatgcaaaa tgccgtcgtt ctggtagtag gtcaactccg tcgcggtgtc gatacgacaa
    32701 cggcagggta cgacttcctg gctaccatcc gcgcgcgtaa gcgtcaccgg aaccgtcgcg
    32761 ccaggttgta gattttgcag atcgccaata tcaatcttct cttccccggt tagccctaac
    32821 gttttacgcg tcacgccttg cggaaattcc agcggcagga tgcccatgcc aattaaattc
    32881 gaacggtgaa ttcgttcaaa cgattcggca atcaccacac gaattccaag cagacgaggt
    32941 ccttttgccg cccagtcacg gcttgagcct gatccatact ctttcccggc aatcaccgcc
    33001 agcggcgttt gctcctgctt atagcgcatc gcagcatcat aaatagagac tacgtcgctg
    33061 tcaggtaaat gccgcgtcat ccccccttca acgccaggca ccatttcatt acggatgcga
    33121 atattggcga aggtgccgcg catcatcact tcatggttac cacgccgcga accgtaggag
    33181 ttaaagtctt ttcgctcaac accccgacct tgtagatatc gacccgctgg gctgtcgggc
    33241 ttaatactgc ccgccggaga gatatggtca gtggtgactg aatcccccag cattgcgagg
    33301 atccgcgcac cgtgaatatc ttccactggt gctggtgttg cctgcatttc atcaaagaaa
    33361 ggcgataagc gaatataggt tgagtcctcc tgccaaccgt aggtatcgga tcgtgtgacg
    33421 ttaatttcct tccactctgc tgtgccttca aaaacttctg cgtactcttt gcggaacatt
    33481 tctgtggaga cttgttctac cgcacgggca atttcttgtg ccgatggcca gatatctttc
    33541 agataaaccg gctcgccttt gcgatcatgg ccgataggct cagaagccag gttgatattc
    33601 atatttcccg ccagcgcata ggcaaccacc agcggtggcg aggccagcca gttagtttta
    33661 accagcggat ggatacggcc ttcaaagtta cggttgccgg acagcaccgc accgacggtt
    33721 aaatcgcctt ttttgattgc cgtttcgata ggatcgggca gcggcccaga gttaccaata
    33781 caggtggtac aaccgtatcc cacaaggtta aaccccagtt cgtcgagata cggtgtcagt
    33841 ttcgcttttg ccagataatc agaaacgact ttcgaacccg gtgccaacga cgctttgacc
    33901 cacggttgcc gcttgaggcc aagagttacg gcttttttcg ccagcaagcc tgcggccatc
    33961 agcacacttg ggttagaggt gttggtgcac gaggttatcg cagcaatgac cacagcgcca
    34021 tcaggtaact gatactgatg tccgttcata acataatcga ctggcaggcg atctttatgc
    34081 gtggcattca cttccagttc gttactggcg gcaaatgctt ttggtacatc gggcagtgca
    34141 acgcgatcct gtgggcgttt tggccctgcc aggctcgctt caacgtcatt catatccagt
    34201 tctaacatac tggtaaaaat cggttcatcg cccgggttac gccacatgcc ctgcgctttg
    34261 gcatattttt cgaccaactc gacctgatct tcgctgcgcc cgcttaaacg catgtaatcg
    34321 agggttactg catcgattgg gaagaagcca caggtggcac catattctgg cgacatattg
    34381 gcaatggtgg cgcgatccgc caacggtagt gaatccagac catcaccata aaattcgacg
    34441 aatttcccca ccacgccatg tttgcgcagc atttgggtaa cagtgagaac caggtctgtg
    34501 gcggtaatac cttcacgtaa ttttccggta agtttaaagc ccactacgtc cgggataagc
    34561 atggaaaccg gctggcctaa catggcggct tctgcttcga tcccacccac gccccacccc
    34621 agcacgccaa ggccgttgat catagtggtg tgcgagtcag taccaacgag tgtatccgga
    34681 taagcaatcc attcaccgtc ctgcaattcg ctccacactg ctttgccgag atattcgagg
    34741 ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc
    34801 tttccccatt tcaggaacac ataacgttcg tggttgcgct ccatttccag gcgtacgttt
    34861 tcttcaaatg cctcatcatc accaaaacga tcgacggtca ccgagtggtc aatgaccagg
    34921 tcgaccggtg agagcgggtt aacctttgca gtatcgccac cgaggcgttt aaccgcttcg
    34981 cgcattgccg ccagatcaac aacggcaggt acgccggtaa agtcctgcat cagcaccctt
    35041 gccgggcggt aggcaatttc acggtcagca tgggcatttt tcagccatcc cgccagtgcg
    35101 tggatatcct cttcggtaac tgagttacca tcctgccagc gcagcaggtt ttcgagcaaa
    35161 actttgagtg acttgggtag acgggtgata tcgcccagtg atttagcagc aagcggcagg
    35221 ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgt ccttactggc ttctcgtagg
    35281 gttgacgaca tagctcctcc ttaatgacag ggttgcgaag ccctgattct cttcagggtg
    35341 aatattaaag ataacacaaa catcgcgtaa cgatttgata acaacccaaa ttgataaaag
    35401 aggagaaacc ttgtggcaaa gcaaatgaca accccgccgc agcggggtca aggatcagtg
    35461 agtaatgatc cagaggagtt cggcccagaa gagcatcgac agagaaaacg cgcccatcca
    35521 ggaccaatat ttcatatttg tgttgatcat aggctacacc cttaacggtt attattttgt
    35581 gttgagataa ctcaagctgt tattgctgcg gtgtattaaa agccctttaa ttcattaaaa
    35641 atgggtaata agaatgatga taattattgt ttcggctgat catcttcagc aaacagttct
    35701 ataaaggctt gctgctgcga tgttagttgc cagtcgtctg gaataacgac ggaacattcc
    35761 cgtttcgttt ctggttcatt gtcactatgc tggcataatg gtgacgactc cagttgctcc
    35821 tgaccgacca tagttatccc cacactttcc aaatcagcag tgcgacaacc acccagaaaa
    35881 gtgcagaacc aagaaaaacc gccagccagg ctttacgttt taattcagga tcacggcggg
    35941 gttcctgatt tccagaaggc attgctaacc tcatataatc gccatcactt atcagcaaga
    36001 cgttgaatga ttggtgtaat taatcatacg atgagacaaa tcctaaagaa actttagtca
    36061 aaaatccagc gaatttacga atcgtctcca gtgaataatt caatcttttg acctgaaata
    36121 gatatttgaa aactggaatt tgcgaggcgg gtaattagac actactttac aggtaattta
    36181 ccctataaat atgagggata aaaatattgt gcacggtata gaaataagta acaaaagaga
    36241 atatattccg gcaccttcgc tacataaaag gtgccgaaaa taacgcaaga aattattttt
    36301 ccggcagttt tatatcttta aacatgacct caatttcttc attggagcgc aatgcgacag
    36361 ccgcatcaac gacatcacgc gttaaatgcg gtgcaaaacg ctgaatgaaa tcatacatat
    36421 aactgcgcaa aaaagtacta cggcgaaaac caattttggt tgtactgtgg ctgaagatat
    36481 cgtgagcatc aacgcgcaca aggtcgggat cggcgaccgg atccaccgcc atgctggcaa
    36541 tgacccctac ccccagccct aaccggacgt aagttttaat gacgtcagca tccgttgccg
    36601 tgaaaacgat acgcggcgtt aaccctgcgc ggttaaaggc agtatccagt tctgaacgtc
    36661 cggtaaagcc gaaggtatat gtcaccaacg gatattgcgc cagttcttca atggtaatgg
    36721 cttttttgcc tgccagcggg tgatccggag tgactacaat agcccgattc cagtggtagc
    36781 acggtaacat cactaaatct tcatacagat gcagcgcttc tgtggcgata gcgaaatcgg
    36841 cattgccttt agagacggca tcagcaattt gtgtcggcgg gccctggtgc atatgcaaag
    36901 aaacgcgggg ataacgctca ataaagcctt tgataacgtt tggtaacgcg tagcgtgcct
    36961 gggtatgcgt ggtggcgata tacagcgagc ctttatccgg ccaggtgtgc tcaccggcaa
    37021 ccgattttat ggcatcgact ttcgacagga cttcgcgagc gatacgaatt atttcttgtc
    37081 ctgctggcgt tacctgcgtc aggtgcttgc cgcttcggga aaaaatttga atgcctagct
    37141 cgtcttccag cattctgact tgtttactga tcccgggttg tgatgtgtaa agtccttccg
    37201 ctgttgatga gacattcagg ttatgattga ccacctcaac aatatagcga agttgttgta
    37261 atttcatgtt aaaccatcca cttagacgcc atcagaacat cgttttttcg tcacacctgg
    37321 cgggtgacta atacgattta ataataaaaa aggtgctaac tataaccact atatcattta
    37381 tagccttagt gtataggtgt gaagaaaaaa taatatgcaa taaaaaaggg ccgctttcgc
    37441 gaccctttgt ttataaaaac ctgacagaat taaaggttat ttttttcctt caacccattt
    37501 gccatcaaca taaaatgctg accagccagt cgcctttccg tctttttccg aagagacata
    37561 ttgctgtttg gttttacggc taaagcgaac catggtctta ttaccttccg gatcctgctg
    37621 tggcgcatcg gccagataac gcagtttttc cggcagacgg tcgcggaagc gataaagctc
    37681 ttccaccagt ggcgcacgcg tttcacgcga tttcgggaaa gtgttggcag ccaggaacac
    37741 accggcagca ccgtcacgca gcacgaaata agcatctgat ttttcgcacg gcagctcagg
    37801 taatggcacc ggatcttctt tcggtggtgc cacttcgccg ttacgtaaaa tcttacgtgt
    37861 gtttttacac tcttcgttgg tgcaggccat gtatttaccg aaacgcccca ttttcaggtg
    37921 catttcagag ccacattttt cacactcaac gatcgggccg tcataacctt taatgcggaa
    37981 ttcgccctct tcgatctcgt aaccgtcgca ggttgggtta ttaccacaga catgcaactt
    38041 acgtttcgga tcgatgagat agctgtccat cgccgtgccg catttcgggc aacgacgttt
    38101 tgcgcgcagc gcgttggttt cagcatcttc gccttccagc acgttcagca cttcgttttc
    38161 cggcaccagg ttaatggtgg ttttgcaacg ctctttcggc ggcagcgcat agccagaaca
    38221 gccaaggaat accccggtac tcgctgtgcg aatccccatt ttgcgaccac aagtcgggca
    38281 gtcaatgctg gtcagaacca tctggttcgg gcgcatacca ccctcttccg gatctttttc
    38341 agctttatct aactgctggg tgaaatccga gaagaagtga tccagtacag ctttccactc
    38401 tgcttcgtga tttgccacct ggtcgaggct gttttccatc tgcgcggtaa agtcgtagtt
    38461 cattaactcg cggaaatttt cttcaaggcg atcggtgacg atttcgccca ttttttccgc
    38521 atagaaacga cgattttcta ctcgcacata gccacgatcc tgaatggtcg aaatgatcga
    38581 cgcataggta gacggacgac cgataccacg tttttccagt tctttaacca gcgatgcttc
    38641 actgaaacgg gctggcggct tggtaaagtg ctgggctggt gttagttcaa cgagcgtcag
    38701 agcatcgcct ttatcgactg ccggtaagat acgatcttca tcgcctttac gcagtgcagg
    38761 catcactttt gtccagccat caaagcacaa aatacgaccg cgtgctttca ggcggaaatc
    38821 gcccgcacca acggtcagcg tcgtggagtc atatttcgct ggggtcatct ggcaggcaac
    38881 gaactgacgc cagattaact ggtacagttt ctgcgcatct gcttccatat ccttcagcga
    38941 ttccgccatc acattgacgt cagaaggacg aatcgcttcg tgcgcttcct gtgagttttc
    39001 tttgctggcg tactgattcg gactttccgg cagatatttc ttaccaaaat tatcgctgat
    39061 ataaccgcga accatattta ccgcgtcctg actcaggtta gtggagtcgg tacgcatgta
    39121 agtgatatag cctgcttcat acaaacgctg cgccatcatc atggtttttt tcacgccaaa
    39181 accaagacgg gtgctggcag cttgttgcag cgtagaggta ataaaaggag cgccaggttt
    39241 actggttgtc ggtttgtctt cacgttccag cacgctgtag cgcgcttttt ccagcagact
    39301 taccgcagcc tgagtttgtt ctttgttgac cggacggaac ggtttgtcat tctgatgagt
    39361 cacctgcaac gccaacgctt caccagatgg cgtagtcgtg ctggcatcga cttcccagaa
    39421 ctcttccggt acgaacgctt taatttcacg ctcacgctct accaccaggc ggaccgccac
    39481 cgactgcaca cgaccggcag acagaccacg agcgatcttt ttccacagca gcggcgaaac
    39541 catatacccc accacgcggt ccataaagcg acgcgcctgc tgggcattaa cacgatcaat
    39601 attcagctca cccggtttgt taaatgcctg gcggatcgcg tttttagtaa tttcgttaaa
    39661 caccactcgg ctatagcgcg catcatcacc accaatcact tcccgcaggt gccatgcaat
    39721 ggcttcccct tcgcggtcaa ggtcggttgc gagatagatg tggtcggctt tttcagccag
    39781 ttgtttcagt tcagagacga ccttctcttt accaggcaac acttcatagt gcgcctccca
    39841 attgtgccac gggtcaaccc ccatacggtt gacgagagcg ccacgttcat cctttttagg
    39901 ctttttagcc gtcttggtgg aggtagagtc ggcactcttt ttggcagctg agccactggt
    39961 cggcaaatcg cggatgtgac cgacgctgga tttcaccacg tagtcacaac ccagatactt
    40021 gttgatcgtt ttggcttttg ccggggactc aacgatgaca agagctttac ccatattcac
    40081 cttacctaat ttgattcttc caggaatgcg tcgcacgttg ataaaccttc cactggcaac
    40141 gagttaccga tatggactcg ctctctgcgg atatcaaccc cttgtcgtga ctctgttcac
    40201 gaaaattcag gtgactgagt taacaggtct tggcctacag gcgctatagc acgacggaat
    40261 tttggtcgaa tgtcaagcaa atctattgcc agattgacga aagcgtcaca ctgtacctga
    40321 taaaatccga tacgcaactt tattagcatg caaaatccaa agttgccagt aaggcgctct
    40381 tgccaccagc gtggtatatt tgtctgcaag ccatgcctgg cgtagactaa tgtccttgtt
    40441 taaggagtaa ataatgaaca aagagacaca acccatcgac cgcgaaacgc tgttgaaaga
    40501 ggccaacaaa atcattcgtg agcatgagga tacgctcgca gggattgaag ccaccggcgt
    40561 cacgcagcgt aatggcgtgc tggtatttac cggcgactac tttttagatg aacaaggatt
    40621 gccaacagcc aaaagcaccg cggtgtttaa tatgttcaaa cacctggccc atgtgctttc
    40681 agaaaaatat catctggttg attaaagcaa aacgcgaggt cttagcctcg cgtttgtctt
    40741 ttacatcaat ggcttttgac cccgctgcca ccagcgtagc aacaatcgat cggcgctctc
    40801 tgccgcgctg ccggtgaatc ggtcaatgag tcgtttacgc tgcatatagc gtacattaac
    40861 cacttcacgg ccttccatca ggctaagaat aacttcatca ctggtgttga tttcatcaac
    40921 caggcctttc tctaccgcct gttgtccgta ccagtgttca cccgttgcca cctgttcaat
    40981 atccagagac ggacgcatac gcttcacaaa atctttaaat aactgatgcg tttcgttcag
    41041 ctcttcgcgg aatttctccc gcccttcttc ggtattttca cccagcaagg tcagcgtgcg
    41101 cttatactgc ccggcggtgt gcagttcgat atcaatatct ttgcttttca ggaagcggtt
    41161 aaagttgggc atttgcgcca ccaccccaat ggaacccaca atagcaaacg gtgcggaaac
    41221 aattttgtcc gccacacagg ccatcatgta accgccgctg gcagcgactt tgtctaccgt
    41281 aacagttaaa ggaatgtttt tatcacgcag acgctgcagc tgcgaagccg ccaacccgta
    41341 accatgcacc atgccaccag ggctttccag acggagcaca acctgatcct gcggtttgaa
    41401 tgctgcgagt accgccgtta tctcttcacg tagcgagttc acttcatggg cgtccatgct
    41461 gcctttaaaa tccagcaccc agacgcgagg tttactgtca gttgccacct cgcccagttt
    41521 ggctttcgct tttgctgctt tcgcttcttg cttgtgcttt ttcttctgcg ctttgtgcca
    41581 ctgtttttgc tgatgtgagt ccatcagcgc cgcggccagt tcttctttca tctccttata
    41641 ctgttcgctg agattgttga cccgtaactc gccacgctgg cgtttattac gttgagcaac
    41701 attgacaata atggcggcaa tcgccgcaat cgctagcaca acggtaacga ttttagccaa
    41761 aaacaaacca tattcagaca acaattccac gagtccccac cttggttaaa caacgctttg
    41821 gctcacagtt tacaacaggc atgttaagcc gtctcgcaga gatgttattg cagcgaaagt
    41881 atgacaaagt atccattttg cggtgttatc gccccaggct gattgaaatg cccttctgtt
    41941 tcaggcataa agccccaaag tcataaagta cactggcagc gcgacagaca acgtcgttga
    42001 ggagtagcca tgcattacca gccaaaacaa gatttactca atgatcgcat tatcctggtg
    42061 acgggagcca gcgatgggat tggtcgtgaa gccgcgatga cgtatgcacg ctatggtgcg
    42121 acagtgattc tgttgggccg taatgaagaa aaattacgtc aggtagccag ccacataaac
    42181 gaagaaactg ggcgtcagcc acagtggttt attctcgatt tgctgacctg cacgtccgaa
    42241 aattgccaac aactggcaca gcgcattgtc gttaattatc cgcgtctgga tggtgttttg
    42301 cataatgccg gattgctcgg cgatgtttgc ccaatgagcg aacaaaatcc gcaggtctgg
    42361 caggacgtca tgcaggtcaa cgttaatgcc acctttatgc tcacccaggc actgcttcct
    42421 ttattactca aatcggacgc cggctcactg gtctttactt catcaagcgt tggacgtcag
    42481 ggacgagcca actggggtgc atatgcagcg tcgaaatttg ccaccgaagg gatgatgcag
    42541 gtactggccg atgaatatca gcagcgcctg cgcgtcaact gcattaaccc aggcggtacg
    42601 cgcaccgcaa tgcgcgcaag cgccttcccg accgaagatc cacagaaact taaaacaccc
    42661 gctgatatca tgccgctcta cctctggctg atgggcgatg acagccgccg taaaaccggc
    42721 atgacctttg acgcccaacc gggccgtaaa ccaggaattt cccaatgagt gatgaacgct
    42781 accaacagcg tcagcagcga gtgaaagaaa aagtagatgc tcgtgtggcc caggcccagg
    42841 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
    42901 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
    42961 aaggcacctg gcctaatggc gaacgcaatc tgctggagcc acatggcgtt gagtttcagg
    43021 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
    43081 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
    43141 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
    43201 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
    43261 atattcttga actggcggat acggtaagtg agctgcgccc catcagacat gcgtttgatg
    43321 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
    43381 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgactgcc gctcacctga
    43441 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
    43501 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
    43561 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
    43621 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
    43681 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
    43741 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
    43801 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
    43861 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
    43921 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
    43981 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
    44041 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
    44101 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
    44161 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
    44221 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
    44281 ggtgtcgcct tcacaggcgt cgaacaggag aatcaatggc gccgtatagc gccattttaa
    44341 ggccgcgtag tatacagagt tacgcactta taagaaaggc ttcacatcac ctacgccttc
    44401 acgcaccacg accggcgtat catcggtaag atcaataacc gttgttggtt tctgcccgag
    44461 atagccgccg tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg
    44521 atcggattcg gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc
    44581 cagtgcttca agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt
    44641 gcgtttctcc tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata
    44701 gttgcccggc gtgttgtttt tcatcaaacg aaacgcaacg ttatccacga atgaataggt
    44761 cgagagttca gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg
    44821 aatacgacaa atacgctcca tggcgttttt atcttcaatt ttacagccga gcgcatagcc
    44881 ggaatcagtt ggatagacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat
    44941 cagacgttgc tgcgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct
    45001 gtggtgttct gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag
    45061 agtttacgac ccagttcgat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc
    45121 cataaatgat gctgacgcgc aagtgtggcc agctgggtgc gttcattggg cgattgctgg
    45181 cactgcgcga cttccatcgc gtcgccgtgg tgttcggcaa aatgcgcaac caatcttttc
    45241 agccatttag cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa
    45301 tgatgaatga catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt
    45361 ttcccgcgcg ccagatactt tttaaagaca tctgccattg aactggcttt gccgcactcc
    45421 acaaggaaac gcgcaaaatg accgcgcgtc actgcgccgc cctgcgccag tcgttgtgcg
    45481 ccttccagcg ccccaggtat ttgcgctttt tcaagtcttt cggcaatttg ctgcgcccgc
    45541 tgattgcgcc gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca
    45601 atattcagcc cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg
    45661 ggaataagat tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cgcgattgca
    45721 gctgtggtgt catggtcggt gatcgccagg gtgccgacgc gcatctcgac tgcacggtgc
    45781 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
    45841 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
    45901 cgacatcata acgggtctgg caaatattct gaaatgagct gttgacaatt aatcatcgaa
    45961 ccagttaact agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt
    46021 actgaaaggt tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa
    46081 tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa
    46141 tgcaaacaca aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc
    46201 cgactgcgct ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg
    46261 cagatatcga cagcaaagat gatttaaaaa gcctgctgct gatagacagt gcgctgcgca
    46321 ttacagcatt aggtgacacc gtcacaattc aggcactttc cggcaatggc gaagccctgc
    46381 tggcactact ggataacgcc ctgcctgcgg gtgtggaaag tgaacaatca ccaaactgcc
    46441 gtgtgctgcg cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc
    46501 tttcggtttt tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac
    46561 gagaagcaat gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt
    46621 taccgcaact gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc
    46681 tgatggtgat tgaccatcag aaaaaaagca cccgtattca ggccagcctg tttgctccga
    46741 atgaagaaga aaaacaacgt ctcactgctc gcctgaacga actacgtcag caactgaccg
    46801 aagccgcgcc gccgctgcca gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga
    46861 gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa
    46921 ttttccaggt ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct
    46981 attacgtgct gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt
    47041 tcaccctatt tggcgcgtcg ccggaaagct cgctcaagta tgatgccacc agccgccaga
    47101 ttgagatcta cccgattgcc ggaacacgcc cacgcggtcg tcgcgccgat ggttcactgg
    47161 acagagatct cgacagccgt attgaactgg aaatgcgtac tgaccataaa gagctgtctg
    47221 aacatctgat gctggttgat ctcgcccgta atgatctggc acgcatttgc acccctggca
    47281 gccgctacat cgccgatctc accaaagttg accgttattc ctatgtgatg cacctcgtct
    47341 ctcgcgtagt cggcgaactg cgtcacgatc ttgacgccct gcacgcttat cgcgcctgta
    47401 tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc tatgcagtta attgccgagg
    47461 cggaaggtcg tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg
    47521 atctcgacac ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc
    47581 aagcgggtgc tggtgtagtc cttgattctg ttccgcagtc ggaagccgac gaaacccgta
    47641 acaaagcccg cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct
    47701 gatggctgac attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca
    47761 gttgcgcagc aatggtcata acgtggtgat ttaccgcaac catattccgg cgcagacctt
    47821 aattgaacgc ctggcgacga tgagcaatcc ggtactgatg ctttctcctg gccccggtgt
    47881 gccgagcgaa gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat
    47941 tattggcatt tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca
    48001 ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt
    48061 tgccggatta acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat
    48121 tccggccggt ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga
    48181 tgcggatcgc gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc
    48241 tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct
    48301 gcaaccgatt ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca
    48361 gctgttttca gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt
    48421 gagcatgaaa attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact
    48481 ggaaaacgca gcgccgttcc cgcgcccgga ttatctgttt gctgatatcg tcggtactgg
    48541 cggtgacggc agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgcggcctg
    48601 tgggctgaaa gtggcgaaac acggtaaccg cagcgtctcc agtaaatccg gctcgtcgga
    48661 tctgctggcg gcattcggta ttaatcttga tatgaacgcc aataaatcgc gccaggcgct
    48721 ggacgagtta ggcgtatgtt tcctctttgc gccgaaatat cacaccggat tccgccacgc
    48781 gatgccggtt cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat
    48841 taacccggcg catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc
    48901 gattgccgaa accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg
    48961 gatggatgaa gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcaaaat
    49021 taagagctat caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact
    49081 ggcaggcgga acacctgaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg
    49141 cgacgccgcc catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg
    49201 ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc
    49261 ttacgacaga gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa
    49321 aatcgtcgca gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag
    49381 ttttcagaat gaggttcagc cgagcacacg acatttttat gatgcgctac agggtgcacg
    49441 cacggcgttt attctggagt gtaaaaaagc gtcgccgtca aaaggcgtga tccgtgatga
    49501 tttcgatccg gcacgcattg ccgccattta taaacattac gcttcggcaa tttcggtgct
    49561 gactgatgag aaatattttc aggggagctt tgatttcctc ctcatcgtca gccaaatcgc
    49621 cccgcagccg attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg
    49681 ctattaccag gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg
    49741 ccagcttgca gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga
    49801 agaggaactg gagcgcgcca ttgcattagg ggcaaaggtc gttggcatca acaaccgcga
    49861 tctgcgtgat ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca
    49921 caacgtaacg gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag
    49981 ccacttcgct aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc
    50041 cgccgtgcgc cgggtgttgc tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga
    50101 tgctaaagca gcttatgacg cgggcgcgat ttacggtggg ttgatttttg ttgcgacatc
    50161 accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta
    50221 tgttggcgtg ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc
    50281 gctggcggca gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga
    50341 agctctgcca gcacatgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc
    50401 ccgcgagttt cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca
    50461 acgttttgac tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg
    50521 cttaggcgca gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa
    50581 ttctgctgta gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca
    50641 gacgctgcgc gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt
    50701 gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa
    50761 gcttttgtca gcgcgcaaaa agatcctgaa tttcaagccc agttcaacga cctgctgaaa
    50821 aactatgccg ggcgtccaac cgcactgacc aaatgccaga acattacagc cgggacgaac
    50881 accacgctgt atctcaagcg tgaagatttg ctgcacggcg gcgcacataa aaccaaccag
    50941 gtgctggggc aggcgttgct ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc
    51001 ggggccggtc agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc
    51061 cgtatttata tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc
    51121 ttaatgggtg cggaagtgat cccggtacat agcggttccg ccacgctgaa agatgcctgt
    51181 aatgaggcgc tacgcgactg gtccggcagt tatgaaaccg cgcactatat gctgggcacc
    51241 gcggctggcc cgcatcctta tccgaccatt gtccgtgagt ttcagcggat gattggcgaa
    51301 gaaaccaaag cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt
    51361 gttggcggcg gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc
    51421 ggcctgattg gtgtggagcc tggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg
    51481 ctaaaacatg gtcgcgtggg catttatttc ggtatgaaag cgccgatgat gcaaaccgaa
    51541 gacgggcaga ttgaagaatc ttactccatc tccgccggac tggatttccc gtctgtcggt
    51601 ccacaacacg cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat
    51661 gaagcccttg aagccttcaa aacgctgtgc ctgcacgaag ggatcatccc ggcgctggaa
    51721 tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga taaagagcag
    51781 ctactggtgg ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt
    51841 ttgaaagcac gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg
    51901 agcgcaaaga aggcgcattc gttcctttcg tcacgctcgg tgatccgggc attgagcagt
    51961 cattgaaaat tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggtatcc
    52021 ccttctccga cccactggcg gatggcccga ccattcaaaa cgccactctg cgcgcctttg
    52081 cggcaggtgt gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc
    52141 cgaccattcc cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg
    52201 agttttatgc ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccag
    52261 ttgaagagtc cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca
    52321 tctgcccgcc aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt
    52381 acacatattt gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc
    52441 tcaatcatct ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg
    52501 gtatttccgc cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt
    52561 ctggttcggc cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg
    52621 cggcactgaa agtttttgta caaccgatga aagcggcgac gcgcagttaa tcccacagcc
    52681 gccagttccg ctggcggcat tttaactttc tttaatgaag ccggaaaaat cctaaattca
    52741 tttaatattt atctttttac cgtttcgctt accccggtcg aacgtcaact tacgtcattt
    52801 ttccgcccaa cagtaatata atcaaacaaa ttaatcccgc aacataacac cagtaaaatc
    52861 aataattttc tctaagtcac atattcctca ggtaattgtt aatatatcca gaatgttcct
    52921 caaaatatat tttccctcta tcttctcgtt gcgcttaatt tgactaattc tcattagcga
    52981 ctaattttaa tgagtgtcga cacacaacac tcatattaat gaaacaatgc aacgcaacgg
    53041 gagaaataac atggccgaac atcgtggtag ttcaggaaat ttcgccgaag accgtgagaa
    53101 ggcatccgac gcaggccgta aaggcggtca gcatagcggc ggtaatttta aaaatgatcc
    53161 gcaacgcgca tctgaagcgg gtaaaaaagg cggtcaacaa agcggtggta ataaatcagg
    53221 caaatcctga ttcaccgtta attattaata tcagctgata aagataattg catctgcggg
    53281 ccgtatggct cgcagtgatt tcactggaga aaaatatgaa tatgaagacc attgaagatg
    53341 tatttattca actgctttca gatacctaca gcgcagaaaa acaattaacc cgggcactgg
    53401 caaaactcgc aagagcaaca tcaaatgaaa aattaagtca ggcttttcat gcgcacctcg
    53461 aggaaactca tggacagatt gaacgaattg atcaagttgt ggagtcagaa tcaaatctga
    53521 aaattaagcg catgaaatgt gtggcaatgg aaggtcttat tgaagaagct aatgaggtca
    53581 tcgaaagtac cgagaaaaac gaagtgcgtg atgccgcact gattgccgca gcacagaaag
    53641 tcgagcatta tgagattgcc agttacggga cattagcgac gctggctgaa caattaggtt
    53701 accgtaaagc agcgaagctt ctgaaagaaa ccctggaaga agaaaaggcc accgacatca
    53761 aactgactga tctggccctt aataacgtaa ataagaaagc cgaaaataaa gcctgaaata
    53821 tgaattttaa cttttagtca ttttataaag aggacatttt catgaatcgt attgaacatt
    53881 atcatgactg gttacgtgac gcccacgcaa tggaaaagca agccgaatct atgcttgaat
    53941 ccatggccag ccgtatagat aattatcctg aactacgcgc tcgtattgaa caacatctta
    54001 gtgaaaccaa aaaccagatt gttcaactgg aaactattct tgatcgtaat gacatttcac
    54061 gttcagtcat taaagattcc atgagtaaaa tggctgcgct tgggcagtca atcggtggta
    54121 tattcccttc tgatgaaata gttaaaggct ctattggcgg atatgtcttc gagcaatttg
    54181 aaatcacctg ttacacctca ctattagcag cagcaaaaaa tgccggtgat acagtctcaa
    54241 ttccgatcat cgaagcgatt ttaaatgagg aaaagcaaat ggccgactgg ctgattcagc
    54301 atattccgca aacaactgag aaatttttaa ttcgttctga aactgatggc gtagaagcga
    54361 agaaataata ccctttatac catgtcctta ttgaccccgt atattacggg gtcgtttttg
    54421 tgcggaatta aaaacgatat cctgctgaga acataaacac ccacggatcg aggcgtacgc
    54481 tatcgtgttg ctgtgcaccg cctagcttat aattggcggt ggtatcgata tccatgtacc
    54541 acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg
    54601 cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg ccatgatcgt
    54661 taaatccatt atcaaagaag gtggtgtagt taataccggc cccaacgtaa ggacggaatt
    54721 tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tggtgaacgg
    54781 ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagta
    54841 attccacacc aatgttgtcg gtcgccatat aagtaaacgt caagcccagt tgagtgttat
    54901 tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta
    54961 cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag gcactgccag
    55021 aaagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt
    55081 tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat cctacataga
    55141 tcacattaca tatgataatt caccatttat tgatctggat taatttagaa gttgcattga
    55201 gaatcctggt taatgtgatt taaatcaatt tttaaatatt ttctctacgc aaatattaac
    55261 tcgatcgcca cttgccgcgg tttgcccacg cctgaaggcc agcaattacc tccgctttac
    55321 aaagatatgc ttttccatac aagacctgat gctgccaccc gaggcttaac cagggtacaa
    55381 ttgcccgcta attaacacct gcataaaact caaggagagt gcatgtctat cacggcgcag
    55441 tccgtatacc gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat tctgttggta
    55501 tcgttgctat gtgcgtttat cacagtggtg ttagggcatg ttttctcacc cagtgatgca
    55561 cagcttgcgc agctcaatga cggcgtgccc gttagcggca gtagtgggtt gttcgacctg
    55621 gttcagaata tgtcaccgga acagcaacaa attttgctgc aggcttcagc ggcgtccact
    55681 ttttcagaat taatcggtaa cgccattctc gccggaggcg taatattaat tatccagctg
    55741 gtgtctgcgg gtcagagagt cagtgcgctg cgagctattg gtgccagtgc gccgatattg
    55801 cccaagttat ttattctgat ttttctgact acccttttag tacagattgg catcatgctg
    55861 gtggtcgttc cgggaattat catggccatt ttactggccc tggcaccggt gatgctggta
    55921 caggacaaaa tgggcatttt tgcctcgatg cgtagcagta tgcggctgac ttgggcgaat
    55981 atgcgtctgg tggcacccgc agtactgagc tggttgctgg caaaaacact gttgctgctt
    56041 tttgcctctt cttttgccgc attaaccccg gaaattggtg ccgtactggc gaacaccttg
    56101 agcaacctga tttctgccat attgctcatc tatctgttcc gcctgtatat gttgattcgc
    56161 caataacctt aagcatttga ttacggaatc gtaaaatgaa gcagtttctt gattttttac
    56221 cgctggttgt ctttttcgcg ttttacaaga tttatgacat ctatgcggct actgcagcgc
    56281 tgatcgtcgc cacggcgatt gtgcttatct atagctgggt tcgctttcgt aaggttgaga
    56341 agatggccct gatcactttt gttctggtgg tcgtcttcgg tggcttgacg ctgttcttcc
    56401 acaatgatga gtttattaaa tggaaggtga cagtcattta tgctctgttt gcgggagccc
    56461 tgttagtcag ccaatgggtg atgaaaaagc cgctaattca gcggatgctg agtaaagaac
    56521 tcacgctgcc gcaaccggta tggtcgaagc taaatctggc ctgggctgtt ttctttatcc
    56581 tttgcggtct ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt tgggtcaact
    56641 ttaaagtctt tggcctgacc gcccttacct taatcttcac attgttaagc ggtatctata
    56701 tctaccgcca catgccgcag gaagataaat cctaaccaga ctgccagtcc ttcggggctg
    56761 gcgctgtatc tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc tgtgattttc
    56821 cttttaagtc ggttttacca tgtctacaac acataacgtc cctcagggcg atcttgtttt
    56881 acgtacttta gccatgcccg ccgataccaa tgccaatggt gacatctttg gtggttggtt
    56941 aatgtcacaa atggatattg gcggcgctat tctggcaaaa gaaattgccc acggtcgcgt
    57001 agtgactgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg gcgatgtggt
    57061 gtgctgctat gcacgctgtg tccagaaagg gacgacatcg gtcagcatta atattgaagt
    57121 atgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga cagaagcatt
    57181 atttaagtat gtagcggttg atcctgaagg aaaacctcgc gccttacctg ttgagtaata
    57241 gtcaaaagcc tccggtcgga ggcttttgac tttctgctta ctgaatttcg gtggtgccgt
    57301 taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc tcataacgcc
    57361 atctgcgcat cgcatttttc acctcacgct caaacatgtt cgcaggcttg gctgagagga
    57421 tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa tttaacttta acctgccctt
    57481 caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt aatgcgcgtg
    57541 gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgccgta cttgatgtgg
    57601 ggcgcgcggg tgccgtattt tcaaacggtg atgccggacg cgactctacg ggtttgacat
    57661 cgcgttttgg ctgctcctgt acctttttca ccggcttcgg ttttggcttc ggtttcggct
    57721 tcggcttttc aatgaccacc ggtgcttctt ttggcggttc ggggatcggc tcaggttccg
    57781 gttctggctc taccaccggc tccggtggcg gctgaacggc ttgtggcggt tcgagatcag
    57841 caggcgcaac catcgtgaca gaaattggtt gcgcaggcgc aggtagttca ataacctgat
    57901 gtaccgaggt atagagcagc cccgccacaa cagcaccatg aatgcagacc gaaagtaacg
    57961 tcggccaggg gaagcggcga ggtaaatcaa gggttattga agtcataatc atttcagtag
    58021 aaaaactagg cctcgatttt aaatgcaaat agcaatcata ttcaataagg caagacgatc
    58081 aggatcagtg aaaatgtcat ttttggcgta attaccctta ctttatgcat gaccttaaca
    58141 aagacatcat ctttccgttg cagatcttcg ccctttcaaa taacgtactt tacaactttc
    58201 ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa gctgatagcc
    58261 tcgaaaaacg cctttccgtt cgtccggcac atttagcacg tttacagtta ctgcatgatg
    58321 aaggtcgttt gttgacagct ggtccaatgc cagcggtaga cagtaacgat ccaggggctg
    58381 cgggatttac tggctcaacc gttattgctg aatttgaatc gctggaagca gctcaggcct
    58441 gggccgatgc tgacccgtac gtagcggcag gagtctatga acacgtttca gtgaaaccat
    58501 ttaagaaagt gttctgagt
//
LOCUS       DAEBXD010000012        58048 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEBXD010000012 DAEBXD010000000
VERSION     DAEBXD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58048
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.012"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(24..278)
                     /locus_tag="KJL94_000746"
     CDS             complement(24..278)
                     /locus_tag="KJL94_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBD4409225.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="KJL94_000747"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="KJL94_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBD4409226.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="KJL94_000748"
     CDS             1228..2694
                     /locus_tag="KJL94_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD4409227.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="KJL94_000749"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="KJL94_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBD4409228.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="KJL94_000750"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="KJL94_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBD4409229.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="KJL94_000751"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="KJL94_000751"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBD4409230.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="KJL94_000752"
     CDS             5709..7265
                     /locus_tag="KJL94_000752"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBD4409231.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="KJL94_000753"
     CDS             7347..9161
                     /locus_tag="KJL94_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD4409232.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="KJL94_000754"
     CDS             9162..10256
                     /locus_tag="KJL94_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBD4409233.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="KJL94_000755"
     CDS             10256..11281
                     /locus_tag="KJL94_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4409234.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="KJL94_000756"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="KJL94_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBD4409235.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="KJL94_000757"
     CDS             complement(12876..13220)
                     /locus_tag="KJL94_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409236.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KJL94_000758"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KJL94_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBD4409237.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KJL94_000759"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KJL94_000759"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBD4409238.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="KJL94_000760"
     CDS             15616..17376
                     /locus_tag="KJL94_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBD4409239.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="KJL94_000761"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="KJL94_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBD4409240.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KJL94_000762"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KJL94_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBD4409241.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="KJL94_000763"
     CDS             19113..19340
                     /locus_tag="KJL94_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBD4409242.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="KJL94_000764"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="KJL94_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBD4409243.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="KJL94_000765"
     tRNA            21195..21271
                     /locus_tag="KJL94_000765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KJL94_000766"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KJL94_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBD4409244.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="KJL94_000767"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="KJL94_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBD4409245.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KJL94_000768"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KJL94_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBD4409246.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KJL94_000769"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KJL94_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBD4409247.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KJL94_000770"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KJL94_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBD4409248.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KJL94_000771"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KJL94_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBD4409249.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KJL94_000772"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KJL94_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBD4409250.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KJL94_000773"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KJL94_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBD4409251.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KJL94_000774"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KJL94_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBD4409252.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KJL94_000775"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KJL94_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBD4409253.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KJL94_000776"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KJL94_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBD4409254.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KJL94_000777"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KJL94_000777"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBD4409255.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KJL94_000778"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KJL94_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBD4409256.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KJL94_000779"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KJL94_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBD4409257.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KJL94_000780"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KJL94_000780"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBD4409258.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KJL94_000781"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KJL94_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBD4409259.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KJL94_000782"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KJL94_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBD4409260.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="KJL94_000783"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="KJL94_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBD4409261.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="KJL94_000784"
     CDS             complement(38328..39347)
                     /locus_tag="KJL94_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBD4409262.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="KJL94_000785"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="KJL94_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="KJL94_000786"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="KJL94_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBD4409263.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="KJL94_000787"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="KJL94_000787"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBD4409264.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="KJL94_000788"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="KJL94_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBD4409265.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="KJL94_000789"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="KJL94_000789"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBD4409266.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="KJL94_000790"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="KJL94_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD4409267.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="KJL94_000791"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="KJL94_000791"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBD4409268.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="KJL94_000792"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="KJL94_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBD4409269.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="KJL94_000793"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="KJL94_000793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBD4409270.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="KJL94_000794"
     CDS             complement(53354..54130)
                     /locus_tag="KJL94_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBD4409271.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="KJL94_000795"
     CDS             complement(54135..55784)
                     /locus_tag="KJL94_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBD4409272.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="KJL94_000796"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="KJL94_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56348..56506)
                     /locus_tag="KJL94_000797"
     CDS             complement(56348..56506)
                     /locus_tag="KJL94_000797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409273.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56503..>58048)
                     /locus_tag="KJL94_000798"
     CDS             complement(56503..>58048)
                     /locus_tag="KJL94_000798"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD4409274.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14199 a        15658 c        14265 g        13926 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat aactcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtctcg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gtcacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaat
//
LOCUS       DAEBXD010000013        55430 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEBXD010000013 DAEBXD010000000
VERSION     DAEBXD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.013"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KJL94_000799"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KJL94_000799"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBD4409275.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KJL94_000800"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KJL94_000800"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBD4409276.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KJL94_000801"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KJL94_000801"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBD4409277.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KJL94_000802"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KJL94_000802"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBD4409278.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KJL94_000803"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KJL94_000803"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBD4409279.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KJL94_000804"
     CDS             7826..9559
                     /locus_tag="KJL94_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBD4409280.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KJL94_000805"
     CDS             9708..10559
                     /locus_tag="KJL94_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4409281.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KJL94_000806"
     CDS             complement(10546..10887)
                     /locus_tag="KJL94_000806"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD4409282.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KJL94_000807"
     CDS             complement(10889..11767)
                     /locus_tag="KJL94_000807"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBD4409283.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11769..14030)
                     /locus_tag="KJL94_000808"
     CDS             complement(11769..14030)
                     /locus_tag="KJL94_000808"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD4409284.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEI"
     gene            complement(14081..14401)
                     /locus_tag="KJL94_000809"
     CDS             complement(14081..14401)
                     /locus_tag="KJL94_000809"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD4409285.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KJL94_000810"
     CDS             complement(14416..15495)
                     /locus_tag="KJL94_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBD4409286.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KJL94_000811"
     CDS             15804..16907
                     /locus_tag="KJL94_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBD4409287.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KJL94_000812"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KJL94_000812"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD4409288.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KJL94_000813"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KJL94_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KJL94_000814"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KJL94_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="KJL94_000815"
     CDS             20366..20983
                     /locus_tag="KJL94_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBD4409289.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KJL94_000816"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KJL94_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBD4409290.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KJL94_000817"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KJL94_000817"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBD4409291.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KJL94_000818"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KJL94_000818"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBD4409292.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KJL94_000819"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KJL94_000819"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBD4409293.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KJL94_000820"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KJL94_000820"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBD4409294.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="KJL94_000821"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="KJL94_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBD4409295.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="KJL94_000822"
     CDS             30130..30738
                     /locus_tag="KJL94_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBD4409296.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KJL94_000823"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KJL94_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBD4409297.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="KJL94_000824"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="KJL94_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBD4409298.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="KJL94_000825"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="KJL94_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBD4409299.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQEHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="KJL94_000826"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="KJL94_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBD4409300.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="KJL94_000827"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="KJL94_000827"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBD4409301.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="KJL94_000828"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="KJL94_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBD4409302.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="KJL94_000829"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="KJL94_000829"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBD4409303.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="KJL94_000830"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="KJL94_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBD4409304.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KJL94_000831"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KJL94_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBD4409305.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="KJL94_000832"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="KJL94_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="KJL94_000833"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="KJL94_000833"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBD4409306.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="KJL94_000834"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="KJL94_000834"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD4409307.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="KJL94_000835"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="KJL94_000835"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBD4409308.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KJL94_000836"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KJL94_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBD4409309.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="KJL94_000837"
     CDS             complement(44853..45152)
                     /locus_tag="KJL94_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBD4409310.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="KJL94_000838"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="KJL94_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="KJL94_000839"
     CDS             45905..46504
                     /locus_tag="KJL94_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBD4409311.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="KJL94_000840"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="KJL94_000840"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBD4409312.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KJL94_000841"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KJL94_000841"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBD4409313.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KJL94_000842"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KJL94_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBD4409314.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KJL94_000843"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KJL94_000843"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBD4409315.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KJL94_000844"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KJL94_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBD4409316.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KJL94_000845"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KJL94_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBD4409317.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="KJL94_000846"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="KJL94_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBD4409318.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="KJL94_000847"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="KJL94_000847"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBD4409319.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KJL94_000848"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KJL94_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBD4409320.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13506 a        15020 c        14159 g        12745 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct agatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggatgca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagagcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAEBXD010000014        54200 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEBXD010000014 DAEBXD010000000
VERSION     DAEBXD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54200
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.014"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            134..433
                     /locus_tag="KJL94_000849"
     CDS             134..433
                     /locus_tag="KJL94_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4409321.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            443..967
                     /gene="ygaP"
                     /locus_tag="KJL94_000850"
     CDS             443..967
                     /gene="ygaP"
                     /locus_tag="KJL94_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBD4409322.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1014..1418)
                     /gene="stpA"
                     /locus_tag="KJL94_000851"
     CDS             complement(1014..1418)
                     /gene="stpA"
                     /locus_tag="KJL94_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBD4409323.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2086..2535
                     /gene="alaE"
                     /locus_tag="KJL94_000852"
     CDS             2086..2535
                     /gene="alaE"
                     /locus_tag="KJL94_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBD4409324.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2572..2916)
                     /locus_tag="KJL94_000853"
     CDS             complement(2572..2916)
                     /locus_tag="KJL94_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBD4409325.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3068..3298
                     /locus_tag="KJL94_000854"
     CDS             3068..3298
                     /locus_tag="KJL94_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409326.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3645..3890
                     /gene="nrdH"
                     /locus_tag="KJL94_000855"
     CDS             3645..3890
                     /gene="nrdH"
                     /locus_tag="KJL94_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBD4409327.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3887..4297
                     /gene="nrdI"
                     /locus_tag="KJL94_000856"
     CDS             3887..4297
                     /gene="nrdI"
                     /locus_tag="KJL94_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBD4409328.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4270..6414
                     /gene="nrdE"
                     /locus_tag="KJL94_000857"
     CDS             4270..6414
                     /gene="nrdE"
                     /locus_tag="KJL94_000857"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBD4409329.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6424..7383
                     /gene="nrdF"
                     /locus_tag="KJL94_000858"
     CDS             6424..7383
                     /gene="nrdF"
                     /locus_tag="KJL94_000858"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBD4409330.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7738..8940
                     /gene="proV"
                     /locus_tag="KJL94_000859"
     CDS             7738..8940
                     /gene="proV"
                     /locus_tag="KJL94_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBD4409331.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8933..9997
                     /gene="proW"
                     /locus_tag="KJL94_000860"
     CDS             8933..9997
                     /gene="proW"
                     /locus_tag="KJL94_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBD4409332.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10055..11047
                     /gene="proX"
                     /locus_tag="KJL94_000861"
     CDS             10055..11047
                     /gene="proX"
                     /locus_tag="KJL94_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBD4409333.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11239..12418
                     /locus_tag="KJL94_000862"
                     /pseudo
     CDS             11239..12418
                     /locus_tag="KJL94_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12542..13279
                     /gene="ygaZ"
                     /locus_tag="KJL94_000863"
     CDS             12542..13279
                     /gene="ygaZ"
                     /locus_tag="KJL94_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBD4409334.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13269..13604
                     /gene="ygaH"
                     /locus_tag="KJL94_000864"
     CDS             13269..13604
                     /gene="ygaH"
                     /locus_tag="KJL94_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBD4409335.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13695..14225
                     /gene="emrR"
                     /locus_tag="KJL94_000865"
     CDS             13695..14225
                     /gene="emrR"
                     /locus_tag="KJL94_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBD4409336.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14352..15524
                     /gene="emrA"
                     /locus_tag="KJL94_000866"
                     /pseudo
     CDS             14352..15524
                     /gene="emrA"
                     /locus_tag="KJL94_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15541..17079
                     /gene="emrB"
                     /locus_tag="KJL94_000867"
     CDS             15541..17079
                     /gene="emrB"
                     /locus_tag="KJL94_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBD4409337.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17143..17658)
                     /gene="luxS"
                     /locus_tag="KJL94_000868"
     CDS             complement(17143..17658)
                     /gene="luxS"
                     /locus_tag="KJL94_000868"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBD4409338.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17808..19364)
                     /gene="gshA"
                     /locus_tag="KJL94_000869"
     CDS             complement(17808..19364)
                     /gene="gshA"
                     /locus_tag="KJL94_000869"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD4409339.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19437..19865)
                     /locus_tag="KJL94_000870"
     CDS             complement(19437..19865)
                     /locus_tag="KJL94_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4409340.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19862..20428)
                     /gene="yqaB"
                     /locus_tag="KJL94_000871"
     CDS             complement(19862..20428)
                     /gene="yqaB"
                     /locus_tag="KJL94_000871"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBD4409341.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20576..20652)
                     /locus_tag="KJL94_000872"
     tRNA            complement(20576..20652)
                     /locus_tag="KJL94_000872"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20616..20618),aa:Arg,seq:acg)
     gene            complement(20717..20793)
                     /locus_tag="KJL94_000873"
     tRNA            complement(20717..20793)
                     /locus_tag="KJL94_000873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20757..20759),aa:Arg,seq:acg)
     gene            complement(20858..20934)
                     /locus_tag="KJL94_000874"
     tRNA            complement(20858..20934)
                     /locus_tag="KJL94_000874"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20898..20900),aa:Arg,seq:acg)
     gene            complement(20999..21075)
                     /locus_tag="KJL94_000875"
     tRNA            complement(20999..21075)
                     /locus_tag="KJL94_000875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21039..21041),aa:Arg,seq:acg)
     gene            complement(21079..21171)
                     /locus_tag="KJL94_000876"
     tRNA            complement(21079..21171)
                     /locus_tag="KJL94_000876"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21135..21137),aa:Ser,seq:gct)
     gene            complement(21487..21672)
                     /gene="csrA"
                     /locus_tag="KJL94_000877"
     CDS             complement(21487..21672)
                     /gene="csrA"
                     /locus_tag="KJL94_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBD4409342.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21907..24537)
                     /gene="alaS"
                     /locus_tag="KJL94_000878"
     CDS             complement(21907..24537)
                     /gene="alaS"
                     /locus_tag="KJL94_000878"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBD4409343.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24665..25165)
                     /gene="recX"
                     /locus_tag="KJL94_000879"
     CDS             complement(24665..25165)
                     /gene="recX"
                     /locus_tag="KJL94_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBD4409344.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25408..26469)
                     /gene="recA"
                     /locus_tag="KJL94_000880"
     CDS             complement(25408..26469)
                     /gene="recA"
                     /locus_tag="KJL94_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBD4409345.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26549..27046)
                     /gene="pncC"
                     /locus_tag="KJL94_000881"
     CDS             complement(26549..27046)
                     /gene="pncC"
                     /locus_tag="KJL94_000881"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBD4409346.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27191..28276)
                     /gene="mltB"
                     /locus_tag="KJL94_000882"
     CDS             complement(27191..28276)
                     /gene="mltB"
                     /locus_tag="KJL94_000882"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBD4409347.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28532..29094
                     /gene="srlA"
                     /locus_tag="KJL94_000883"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28532..29094
                     /gene="srlA"
                     /locus_tag="KJL94_000883"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29091..30050
                     /gene="srlE"
                     /locus_tag="KJL94_000884"
                     /gene_synonym="gutE"
     CDS             29091..30050
                     /gene="srlE"
                     /locus_tag="KJL94_000884"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBD4409348.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            30061..30432
                     /gene="srlB"
                     /locus_tag="KJL94_000885"
                     /gene_synonym="gutB"
     CDS             30061..30432
                     /gene="srlB"
                     /locus_tag="KJL94_000885"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBD4409349.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30436..31215
                     /gene="srlD"
                     /locus_tag="KJL94_000886"
     CDS             30436..31215
                     /gene="srlD"
                     /locus_tag="KJL94_000886"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBD4409350.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31320..31679
                     /gene="gutM"
                     /locus_tag="KJL94_000887"
                     /pseudo
     CDS             31320..31679
                     /gene="gutM"
                     /locus_tag="KJL94_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            31746..32519
                     /gene="srlR"
                     /locus_tag="KJL94_000888"
                     /gene_synonym="gutR"
     CDS             31746..32519
                     /gene="srlR"
                     /locus_tag="KJL94_000888"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBD4409351.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32512..33477
                     /gene="gutQ"
                     /locus_tag="KJL94_000889"
     CDS             32512..33477
                     /gene="gutQ"
                     /locus_tag="KJL94_000889"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBD4409352.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33474..34988)
                     /gene="norR"
                     /locus_tag="KJL94_000890"
     CDS             complement(33474..34988)
                     /gene="norR"
                     /locus_tag="KJL94_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBD4409353.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35175..36614
                     /gene="norV"
                     /locus_tag="KJL94_000891"
     CDS             35175..36614
                     /gene="norV"
                     /locus_tag="KJL94_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBD4409354.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36611..37744
                     /gene="norW"
                     /locus_tag="KJL94_000892"
     CDS             36611..37744
                     /gene="norW"
                     /locus_tag="KJL94_000892"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBD4409355.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37872..40124)
                     /gene="hypF"
                     /locus_tag="KJL94_000893"
     CDS             complement(37872..40124)
                     /gene="hypF"
                     /locus_tag="KJL94_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBD4409356.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40277..40804)
                     /gene="hydN"
                     /locus_tag="KJL94_000894"
     CDS             complement(40277..40804)
                     /gene="hydN"
                     /locus_tag="KJL94_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBD4409357.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40953..41963)
                     /gene="ascG"
                     /locus_tag="KJL94_000895"
     CDS             complement(40953..41963)
                     /gene="ascG"
                     /locus_tag="KJL94_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBD4409358.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41977..42096)
                     /locus_tag="KJL94_000896"
     CDS             complement(41977..42096)
                     /locus_tag="KJL94_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBD4409359.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(42106..43815)
                     /gene="hycE"
                     /locus_tag="KJL94_000897"
     CDS             complement(42106..43815)
                     /gene="hycE"
                     /locus_tag="KJL94_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBD4409360.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43833..44756)
                     /gene="hycD"
                     /locus_tag="KJL94_000898"
     CDS             complement(43833..44756)
                     /gene="hycD"
                     /locus_tag="KJL94_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBD4409361.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44759..46552)
                     /gene="hycC"
                     /locus_tag="KJL94_000899"
     CDS             complement(44759..46552)
                     /gene="hycC"
                     /locus_tag="KJL94_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD4409362.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGS
                     VWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSN
                     SGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVS
                     VVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMA
                     ICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVP
                     GWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(46589..47200)
                     /gene="hycB"
                     /locus_tag="KJL94_000900"
     CDS             complement(46589..47200)
                     /gene="hycB"
                     /locus_tag="KJL94_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBD4409363.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(47325..47786)
                     /gene="hycA"
                     /locus_tag="KJL94_000901"
     CDS             complement(47325..47786)
                     /gene="hycA"
                     /locus_tag="KJL94_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBD4409364.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47998..48348
                     /gene="hypA"
                     /locus_tag="KJL94_000902"
     CDS             47998..48348
                     /gene="hypA"
                     /locus_tag="KJL94_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD4409365.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            48352..49224
                     /gene="hypB"
                     /locus_tag="KJL94_000903"
     CDS             48352..49224
                     /gene="hypB"
                     /locus_tag="KJL94_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBD4409366.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            49215..49487
                     /gene="hypC"
                     /locus_tag="KJL94_000904"
     CDS             49215..49487
                     /gene="hypC"
                     /locus_tag="KJL94_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBD4409367.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            49487..50608
                     /gene="hypD"
                     /locus_tag="KJL94_000905"
     CDS             49487..50608
                     /gene="hypD"
                     /locus_tag="KJL94_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBD4409368.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            50605..51615
                     /gene="hypE"
                     /locus_tag="KJL94_000906"
     CDS             50605..51615
                     /gene="hypE"
                     /locus_tag="KJL94_000906"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBD4409369.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51689..53767
                     /gene="flhA"
                     /locus_tag="KJL94_000907"
     CDS             51689..53767
                     /gene="flhA"
                     /locus_tag="KJL94_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBD4409370.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53804..54157)
                     /locus_tag="KJL94_000908"
     CDS             complement(53804..54157)
                     /locus_tag="KJL94_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBD4409371.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        12890 a        14615 c        13888 g        12807 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctattcag cttacgcttt tactctcatt
       61 tacgctgctg aggctggtat atgttgcatt tttagtttag agtttactta atttagaaaa
      121 tacttaaata aatatgactg aactcgcgca attacaggcc agtaccgaac aggcagcggc
      181 cttattgaaa gcaatgagcc accctaaacg gttgctgatt ctgtgcatgc ttagcggttc
      241 ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagcgcct ctgcgacatc
      301 acagcatctc gctcgtatgc gggacgaagg gcttatcaac agccaacggg atgcccaacg
      361 cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt
      421 ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga
      481 attaatcgca cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga
      541 acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc
      601 taaattacgt cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa
      661 cgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat
      721 tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt
      781 aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta
      841 taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc
      901 aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg
      961 agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc
     1021 aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg
     1081 ccctgaccgg tccaggtttt agtttcaccg ttaacatcag tgaatttata tttcgccgga
     1141 cgcggctggc gttttttacc agcacgtggt gcagcagcag agctattacc caataactct
     1201 tccgggttaa ttccgtcagc tttcatcagc tccagccagg tgctaatttt ttcctgacgc
     1261 tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg
     1321 aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg
     1381 gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc
     1441 caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa
     1501 tttaatctat ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctcag
     1561 aataatttca agattatata ttagccattt tgatgagtaa tttaggttat tggtgaacag
     1621 agtggcgctc tctgctcgct aactccgcaa gcctgacaaa accgaagtga ataaaaatgc
     1681 aatataacag aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt
     1741 tctggcatca aaaatgctgt aaatgacccg gtcacgacaa acaccgaatt ctctacgctt
     1801 atagcgttac ctccccccca aactttcatt agtgaaatta aaggcaagat catatctaat
     1861 tgatttaatt gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaacgag
     1921 ccgttacgga atattttatc tacaaaaact aactaaataa aaatttttca ctaattgatt
     1981 agtcatagac agcgatatac gctatgcaaa aatgcagatg gcaatgagat ccactgcttt
     2041 catctccatt aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag
     2101 tcacgcttgc gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg
     2161 aacatgtgta ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga
     2221 ttggtagcga ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg
     2281 tttatgcgcg cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggctgatatc
     2341 ctggcttatg tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca
     2401 gactggcatc agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg
     2461 gcggtttatg gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag
     2521 caggtaaaag cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc
     2581 ttcgccaaat aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg
     2641 gataccgtaa tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc
     2701 gcaagtttta atatcggaag cgggttcagc cagtgttgaa ctgcgttctt ttaatgttgg
     2761 atgaattacc agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc
     2821 cccacggcgg taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc
     2881 aagtacgcgc gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attttcaacc
     2941 gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc
     3001 gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg
     3061 ggatcatatg tataaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca
     3121 gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga
     3181 cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcgttgctga aagaaaccag
     3241 ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgagc
     3301 ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat
     3361 ttttatcggc gcactgttaa gcgtgcgcaa atcgtagtgc aaaaatgata ataaatacgc
     3421 gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc
     3481 ccctcccccg tcacgctcat atccagggta atttcgatca ctatttgcta tatattgtgt
     3541 ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg
     3601 cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc ataactattt
     3661 atactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct
     3721 ttgattttga aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc
     3781 agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc
     3841 cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt
     3901 ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc
     3961 ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat
     4021 cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt
     4081 tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa
     4141 ctttggtgag gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg
     4201 gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt
     4261 aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac
     4321 gcgctgaatg cgatgcttaa tctttacgat agcgcaggtc gcattcagtt cgataaagac
     4381 cgccaggccg ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt
     4441 agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt
     4501 aatcgctact ctcgcgactt tgtcattacg ctgtttgccc acgcacacac cagcggtttt
     4561 cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc
     4621 gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg
     4681 gcacaaggcg atgagacgct ggcgttgcaa ctgaccgatg aaatgctgtc aggacgcttt
     4741 cagccagcca cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc
     4801 tgttttttgc tgcgtattga agacaatatg gagtcgattg gtcgggcggt aaattccgca
     4861 ctgcagctgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgggaagcg
     4921 ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg
     4981 ttggaagacg cattttccta tgccaaccaa ctcggcgcgc gtcagggggc aggtgcagtc
     5041 tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc
     5101 gacgaaaaaa tccgcattaa aacactgtcg ctgggcgtgg tgatcccgga tatcactttc
     5161 catctggcaa aagagaatgc gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt
     5221 tatggcaagc cgtttgccga tatcgccatt agcgaacact atgacgaact ggttgccgat
     5281 gaacgcatcc gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc
     5341 cagtttgagt ccggctatcc ctacatcatg tatgaagaca cagtaaaccg tgctagccct
     5401 atcgccgggc gcataaatat gagtaatctc tgctcagaaa ttttgcaggt taacagcgcc
     5461 tcagagtatg acgagaatct cgactatgcc cgcacaggcc atgatatttc ctgcaattta
     5521 ggttcgttga atattgcgca caccatggat tcccccgatt tttcccgcac ggtagagact
     5581 gccgtgcgcg gtttaacggc ggtgtcagat atgagtcata tccgcagcgt gccgtccatc
     5641 gaagccggaa atgccgcctc gcacgccatc ggactggggc agatgaattt acatggttat
     5701 ctggcgaggg aaggcatcgc ttatggttcg ccggaagccc tggatttcac caatctctat
     5761 ttctatacca tcacctggca cgcactgcgt acctcgatgt tgctggcgcg cgaacgcggt
     5821 gaaaccttcg ccgggttcaa acagtcacgc tatgccagtg gtgaatattt cagccaatat
     5881 ctgcaaggta actggcagcc gaaaacggcg aaagttggcg aactgtttgc ccgcagctgt
     5941 attacgttac ctacccgtga gatgtgggcg caactgcgcg acgacgtgat gcgctacggc
     6001 atatacaacc agaatctcca ggcggtgccg ccaaccgggt ctatctctta tatcaaccat
     6061 gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca
     6121 ggacgcgttt actaccctgc cccgtttatg actaacgaaa atctggcgct gtatcaggac
     6181 gcttacgaaa ttggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc
     6241 gatcaggggc tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac
     6301 aaagcgcaga tttacgcctg gcgcaaaggt atcaaaacgc tctattacat ccgcctgcgt
     6361 cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata
     6421 tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat
     6481 ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg
     6541 aacgatattc ccgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt
     6601 tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg
     6661 cccgatgcgc tcacgcctca tgaagaggcg gtattatcga atatcagctt tatggaagcg
     6721 gttcatgccc gctcttacag ttcgattttc tcgacgctgt gccagaccaa agatgtcgat
     6781 gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag
     6841 caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct
     6901 tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc
     6961 aataccgcgg acctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata
     7021 ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag
     7081 agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag
     7141 ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacaccaat
     7201 aaggctttga tgaatctggg ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat
     7261 ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt
     7321 tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc
     7381 tgagggtgtt attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata
     7441 gctttttatc acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc
     7501 ctgaaactac aatattcagg cgttttttcg ctatctatga caaaaaatat caacttttct
     7561 cgatttgctc tcagccctta tatcacggaa aattccggcg atttgctcgc atcaatattc
     7621 atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt
     7681 gactattccc attgggtaat atatcgacat aaacaaataa aggaatcatt ctattgcatg
     7741 gccattaaat taaaaattaa aaatctttat aaaatatttg gtgagcatcc acagcgagcg
     7801 ttcaaatata tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac tgggctatcg
     7861 cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga
     7921 ttatccggct cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc
     7981 cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc cgaactccgt
     8041 gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg
     8101 accgtgctgg acaatactgc gttcggtatg gaattggccg gaattaatgc cgaagaacgc
     8161 cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac
     8221 ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt
     8281 aatccggata tattattaat ggacgaagcc ttctcggcgc tcgatccatt aattcgcacc
     8341 gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt
     8401 tcccacgatc ttgatgaagc catgcgtatt ggcgaccgaa ttgccattat gcaaaatggt
     8461 gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc
     8521 cgtaccttct tccgtggcgt tgatattagt caggtgttca gtgcgaaaga tattgcccgc
     8581 cggacaccga atggcttaat tcgtaaaact cctggcttcg gcccacgttc ggcactgaaa
     8641 ttattgcagg atgaagatcg cgaatatggc tacgttatcg aacgcggtaa taagtttgtc
     8701 ggcgcagtct ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg tcttgatgcg
     8761 gcgctgattg atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga gttgctctct
     8821 catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc
     8881 atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga
     8941 tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcgcaat ccgcagacgc
     9001 ctggggtaca ccgacgactg caccggctga cggcggtggt gctgactggc tgaccagtac
     9061 gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc
     9121 gctcgacagt tgggtcactg aagggatcga ctgggttgtt acccatttcc gtcccgtctt
     9181 ccagggcgtg cgcgttccgg ttgattatat cctcaacggt ttccagcaat tgctgctggg
     9241 tatgccagcg ccagtggcga ttatcgtttt cgctctcatc acctggcaga tttccggggt
     9301 cggaatgggc gtggcgacgc tggtctcgct gattgccatc ggcgcaatcg gtgcctggtc
     9361 gcaggcaatg gtgactctgg cgctggtgtt aactgccctg ctgttctgta tcgtcatcgg
     9421 tttgccgttg gggatatggc tggcgagaag tccgcgagcg gcgaaaatta ttcgtccact
     9481 gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtgccaatcg tcatgctatt
     9541 tggtatcggt aacgtgccgg gcgtggtggt gacgatcatc tttgcgctgc cgccgattat
     9601 ccgtctgacc attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc
     9661 attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac
     9721 cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg tcattgcctc
     9781 gatgattgcc gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc gtctggatat
     9841 ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgatcgcct
     9901 gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg
     9961 ccctgttggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc cggtggcgcc
    10021 gggcattatc accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac
    10081 agcgtttgcc acgcttatct ctacacaaac ttttgctgcc gatctgccgg gcaaaggcat
    10141 tactgttaat ccagttcaga gcaccatcac tgaagaaacc ttccagacgc tgctggtcag
    10201 tcgtgcgctg gagaaattag gttataccgt caacaaaccc agcgaagtgg attacaacgt
    10261 tggctacacc tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact ggacgccact
    10321 ccatgacaac atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggggtatt
    10381 tgttaacggc gcagcacagg gttacctgat cgataagaaa accgccgacc aatacaaaat
    10441 caccaacatc gcacaactga aagatccgaa gatcgccaaa ctgttcgata ccaacggcga
    10501 cggtaaagcg gatttaaccg gttgtaaccc tggctggggc tgcgaaggtg cgatcaacca
    10561 ccagcttgcc gcgtatgaac tgaccaacac cgtgactcat aatcagggga actacgcagc
    10621 gatgatggcc gacaccatca gtcgctacaa agagggcaaa ccggtgtttt attacacctg
    10681 gacgccgtac tgggtgagta atgagctgaa gccagggaaa gatgtggtct ggttgcaggt
    10741 gccgttctcc gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa
    10801 ttacggcttc ccggtcagca ccatgcatat cgttgccaac aaagcctgga ccgagaaaaa
    10861 tccggcggca gccaaactgt ttgccattat gcagttgcca gtggccgata ttaacgccca
    10921 gaacgccatt atgcatgacg gcaaagcctc agaaggcgat attcagggcc acgttgatgg
    10981 ctggatcaaa gcccaccagc agcagttcga tggctgggtg aatgaggcgc tggcagcgca
    11041 gaagtaattt ttattcgggc ggataaggcg ttttcgcctc atccgccgct ctgtacacaa
    11101 tgcctgatgc gacgctgacg cgtcctatca tgcctacaaa ccacatcgca catttcaaca
    11161 agctatcttt catcccatat tcatcaacat ccgctattat tgatttccag cttaatcatc
    11221 acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc gctgatcgtg
    11281 ctgatgtcta tcgccaccgg tctggcggta gccagtaact attacgccca gccattgctc
    11341 gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat tgttaccgcc
    11401 gcgcagttgg gctatgccgc aggtctactg tttcttgttc ccctcggtga tatgtttgaa
    11461 cgccgccgcc tgattgtctc gatgacctta ctggcagcgg gcggtatgtt aatcactgcc
    11521 agcagtcagt cactggcgat gatgatcctc ggtacggcat taaccggttt attctcagtc
    11581 gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa acgcggcaaa
    11641 gtggttggca ccattatgag cggtctgctg ttggggatat tgctggcacg aaccgttgcc
    11701 gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcttc ggtgttaatg
    11761 gcactgatgg cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga aacccacctc
    11821 aactacccac agttgttggg ttccgttttc agcatgttca tcagcgataa gatcctgcgc
    11881 acccgcgcgt tgctgggctg catgaccttt gccaacttca gcattctctg gacctcaatg
    11941 gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgacattgg tctgtttggg
    12001 cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc cgacaagggc
    12061 aaatcgcacc acaactttcg gtctgcttct gctgttactt tcatggctgg caatctggtt
    12121 tggtcacact tccgtactgg cgttgattat cggcattctg gtgctggacc tcaccgtaca
    12181 gggcgtgcat atcactaacc agacggtaat ttatcgaata catcctgatg cgcgtaatcg
    12241 cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc
    12301 agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc
    12361 cctggttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc
    12421 aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg
    12481 ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc
    12541 tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa
    12601 agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc
    12661 gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg
    12721 cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc
    12781 actgagcgtc atggcaatgg atgttcgcca tgttttgtat ggcccgtcac tgcgtagccg
    12841 tattattcag cgtctgcaaa aatcgaaaac cgccctatgg gcgtttggcc tgacggatga
    12901 ggtttttgcc gccgccaccg caaaactggt acgcaataat cgccgctgga gcgagaactg
    12961 gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc
    13021 attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat taggttttat
    13081 gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg
    13141 cgttaccgca gctttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc
    13201 cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg
    13261 agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt
    13321 gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg
    13381 cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct
    13441 ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg
    13501 cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg
    13561 cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt
    13621 ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat
    13681 gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg
    13741 ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca
    13801 tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga
    13861 cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg
    13921 aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg
    13981 aaaaacgcgg ttggatcgaa cgtcgtaaaa gcgataacga tcgccgctgc ctgcatctgc
    14041 aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc
    14101 tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc
    14161 gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga
    14221 gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca
    14281 tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg
    14341 tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga
    14401 gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag
    14461 cgatagggat ttattggttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg
    14521 tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg
    14581 ccgataacac cgattttgta aaagaaggcg atgttctggt cactctcgac ccgacagatg
    14641 ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc
    14701 agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa atcgccctcg
    14761 cgaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc
    14821 gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg
    14881 ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc
    14941 ctgccgtgca acaggctgcc accgaagtac gtaacgccta gctggcgctg gagcgtaccc
    15001 gtattgtcag tccgatgacc ggttatgttt cccgtcgtgc cgtgcaaccg ggtgcgcaaa
    15061 ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca
    15121 actttaaaga gacgcagatt gccaatatgc gcatcggtca gccggtcacc atcaccaccg
    15181 atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag
    15241 gtagcgcgtt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc
    15301 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
    15361 ttggtttgtc cacgctggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
    15421 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
    15481 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
    15541 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
    15601 ctggcgacat tcatgcaggt gctgaactcc accattgcta acgtggcgat ccccactatc
    15661 gccgggaatc tgggctcatc gctcagccaa ggaacgtggg taatcacttc tttcggggtg
    15721 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
    15781 ctgttccttt ggtccaccat cgcctttgct attgcgtctt gggcgtgtgg tgtctccagc
    15841 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
    15901 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
    15961 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
    16021 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
    16081 gtggtgttga tgacactgca aaccctgcgc ggacgtgaaa cccgcaccga acggcggcgg
    16141 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
    16201 gaccgcggta aagagctgga ctgtttttca tcacaggaaa ttatcattct taccgtggtg
    16261 gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc
    16321 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
    16381 tatatgctct acttcgacgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt
    16441 tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgattctg
    16501 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
    16561 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
    16621 ttttgcgcgt cggcctggcc gcagtttatc caggggtttg cggtggtctg cttctttatg
    16681 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
    16741 ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg
    16801 tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac
    16861 ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca
    16921 tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc
    16981 ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg
    17041 ccatttggcg caggtggcgg cggaggcggt gcgcactaag tacaactaag ccagttcatt
    17101 tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg
    17161 caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacct cacgttccag
    17221 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
    17281 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
    17341 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
    17401 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
    17461 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
    17521 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
    17581 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
    17641 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
    17701 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
    17761 tcagagatga aattttggcc actcccgagt ggcctttttc ttttctgtca ggcgtgtttt
    17821 tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca
    17881 gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
    17941 agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc
    18001 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
    18061 tcatcacaca ctttctgata cgcttcgccg ccgttaatgc tatccagcgt ttgcgcgacg
    18121 cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg
    18181 cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta
    18241 acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
    18301 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
    18361 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
    18421 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
    18481 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
    18541 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
    18601 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
    18661 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
    18721 gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
    18781 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
    18841 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc
    18901 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
    18961 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
    19021 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
    19081 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
    19141 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
    19201 ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
    19261 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
    19321 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
    19381 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
    19441 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
    19501 ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc cgctaacaga
    19561 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
    19621 ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
    19681 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
    19741 acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
    19801 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
    19861 ctcacagcaa gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc tgaataccga
    19921 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
    19981 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg acgacggcgt
    20041 caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
    20101 ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca acaagaggaa
    20161 gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
    20221 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
    20281 gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata
    20341 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
    20401 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
    20461 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
    20521 gataaagtag ttatttagaa atgagatatt tttgaaggaa attttttgga gaatatggtg
    20581 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    20641 agctacggat acatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc
    20701 cgtatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    20761 gccgagtact ctatccagct gagctacgga tgcatcggga aacttacttt actgcagatt
    20821 ttttgatacc gctactaaag ccatatcaag taagagatgg tgcatccggg aggattcgaa
    20881 cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcatcggg
    20941 aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa gtaagagatg
    21001 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    21061 ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg cagcttttga
    21121 ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac cacacgcctc
    21181 ttacgagtgc ttcgaagaac ttgtttatcg ctcatcatcg ctgcgtggcg cacatattac
    21241 tttctgagac ttataagtca aacaattttt cccacacttt tatcgtttgc acacttcacg
    21301 ctcaattagt ctgtaaaagc gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg
    21361 gcagatagca gtaatgcaaa aaaatggaga cttaagttga atgaacggga gtaaagcgaa
    21421 aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag atgagacgcg
    21481 gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt agatctcttc
    21541 acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt tgccctttac
    21601 ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt
    21661 cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg
    21721 cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca tgtaagcagt
    21781 catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt
    21841 tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg
    21901 cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa
    21961 ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc
    22021 cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt
    22081 agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt
    22141 cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc
    22201 gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt
    22261 tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg
    22321 cgtacgttcc agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag
    22381 atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc
    22441 ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa
    22501 cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc
    22561 gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc
    22621 tttcgctttc gccgcttcga gattcatgat gttggtttcg atcggcaaat tgcgacgaat
    22681 ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg
    22741 tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt
    22801 ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat
    22861 acgggcgcga cgagcctcat caatatcagc ctgcacagcg tcgcccactt tcagagaacc
    22921 cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc
    22981 gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc
    23041 atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga tggcatcaac
    23101 cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc
    23161 tttaaattca gatgcactat caacacggat cattgcgttg taatcggcac caaagccgct
    23221 ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac
    23281 tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc
    23341 gtacagacgg aaagccgttt caccatccag cgtatcacca gaaagttttg ccagctcttc
    23401 atccagcaac gccagaccgc gctccagagt acgggcaaac tgctcttctt cagtcttcag
    23461 cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc
    23521 gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattgccgtg
    23581 acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat
    23641 tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga
    23701 tttattgctc agatcggtcg cgccagtgac tttcgctacc gcctggatca acgtgcggaa
    23761 caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc
    23821 cataccggta tctacagacg gcttcggcag cggttccatc gtgccatctg cctggcggtt
    23881 gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact
    23941 tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc
    24001 gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt
    24061 atcgccgatg cgaataatac gttcgcgcgg gatcccgact tctttttccc agatttcgta
    24121 ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca
    24181 tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc
    24241 gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt
    24301 ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga
    24361 ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt
    24421 aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc
    24481 cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat
    24541 tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg
    24601 ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat
    24661 gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat
    24721 agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat
    24781 attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg
    24841 ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctgacga atacgtgcag
    24901 gtccataccc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga
    24961 gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa
    25021 tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct
    25081 cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg
    25141 ggcgacggga tgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc
    25201 gcagatgcgc cccaggtttt gctgataaac agatttaata gtcaatgagt atgcagaatg
    25261 ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgctg
    25321 gcctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca
    25381 ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctactccttc
    25441 gctgtcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac
    25501 tttcttctcg atctctttcg cggtttccgg gttatctttc agccaggcag ttgcattcgc
    25561 tttaccctga ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag
    25621 cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag
    25681 gatctgaaat tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg
    25741 ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag
    25801 acgaacagag gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa
    25861 catcacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt
    25921 caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg
    25981 agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac
    26041 gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc
    26101 gccggtatcc ggctgggagc acagcaggtt atcgatatcg acgcccagtt tacgtgcgta
    26161 gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc
    26221 tgcggcgatc acctgcagcg tcagcgtggt tttaccggaa gattccggtc cgtagatttc
    26281 gacgatacgg cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc
    26341 ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt
    26401 accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc
    26461 gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga
    26521 aacaattata ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg
    26581 tctgcaatgc atacgcagta gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt
    26641 cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag
    26701 gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag
    26761 cacgagccgc tttcagtgca cctatcgcca tttccaccac gacgggttca ctcaccgcgc
    26821 catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt
    26881 aggtgacaaa tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg
    26941 ctacccaacc accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg
    27001 cctgcccaac ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca
    27061 gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca
    27121 attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc
    27181 ttgtaaatag ctactgtact cgcgccagcg ccacggcttg tcctaactgc cagaccgcca
    27241 ttgcgtaatg ggtgctgtgg ttgtaacggg tgatggtgta gaagttcggc agaccgtacc
    27301 agtactggta gccggtgcca acatccagac gcagcaggct ggcttgttga tggttgccca
    27361 gcggctgctg tggcgttaaa cctgcggcgg caagctgcga aatgctgtac ttagttttga
    27421 aaccatttgg caagcctgga gcctgaccgt ttgccattat cgcgacctga tcgcctttca
    27481 cccagccgtg cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc ggatcccaca
    27541 ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa
    27601 actgtccgta gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct
    27661 cgtcgcgcgc catcagcagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg
    27721 ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc
    27781 cccagcgggt ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct
    27841 gccacgcgcg attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt
    27901 tgtccggcgt aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg
    27961 gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgct
    28021 tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt
    28081 tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat
    28141 tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag
    28201 taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat
    28261 aacgacgctt gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa
    28321 gcatcacacc ccgcacaagg aagcggtagt cactgcccga tacggacttt acataactca
    28381 actcattccc ctcgctatac ttttattcaa actttcaaat taaaatattt atctttcatt
    28441 ttacgatcaa aataacactt ttaaaccttt caacctgatt agattaggtt gccgtttggt
    28501 aataaaacaa taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga
    28561 gtggtttatc gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat
    28621 tcttccgctg ttgattagcc tgcaggttat catgaacgca ctgattaatt ttatcggtca
    28681 gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctact
    28741 gttaccgtgc attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt
    28801 tatgccggaa aagtacaaac ccagctacta cgcggcggcc tcttatagct gccactcaat
    28861 gaatggcctc ttcccccata tcaaccctgc gaactgtttg tttatcttgg cattgccagc
    28921 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagttatct gctggttggt
    28981 ctggtcacca atttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa
    29041 aagatgggca ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta
    29101 ttcggatcga aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg
    29161 gcaaaaaaat cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac
    29221 aacttactgg ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa
    29281 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac
    29341 gtattcccac cattaatatc cactcgacgg gcaagtccgg tccgctggcg cagtacattg
    29401 tggaagatat ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
    29461 caccacaacc ctcttccgtg ggccgtgact atgacaccag taagaaaatc accgaacaaa
    29521 gcgatggttt actggcgaag gtgggaatgg gcatggggtc cgccgttgcc gtgttgtttc
    29581 agtctggtcg tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg
    29641 tctcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
    29701 ttgctccgct agcgagccat ccactgggtc tggtcatgct agcgctcatc tgctccttcc
    29761 cactgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
    29821 ttggcgtgca gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg
    29881 ccatcaacgc gcaggcggct tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
    29941 gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg
    30001 cgccgactgt actgatcgcc tggtttgtct ccagttttat ctatcaatag aggctgaaac
    30061 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
    30121 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc
    30181 ttcattcatt gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcactc
    30241 gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa
    30301 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
    30361 catgtggcag gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
    30421 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
    30481 gcgttcctgt gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag
    30541 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggtatggcg
    30601 tacggttttg gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta
    30661 gatgaaatct ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc
    30721 tttatcagcg acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt
    30781 tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc
    30841 attattcaga tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc
    30901 gcagcgaaat ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac
    30961 ggcattacgg tacattcact gatgctcggt aacctgctga aatcgcggat gttccagtca
    31021 ctgttgccac aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac
    31081 atcgacaaag taccgctcaa acgcggctgc gattatcaag atgtgctgaa tatgctgctg
    31141 ttctacgcca gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt
    31201 caggtgatgt tctgatcaac agcggagatc cgttaaggat ctccgtgaga ctatagaatg
    31261 cctgatgcgc tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta
    31321 tggtatccgc actcatcacc gtcgccgtta tcgcctagtg tgcgcaactg gccttaggcg
    31381 gctggcaaat ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg
    31441 gcgtgggccg ttccagcggg cgctttaaac cgcgggtcgt ggtcgccatc gcgctggacg
    31501 atcagcagtg catcgtcgac accttgttta tgaaaggact gactgtcttc gcccgaccgc
    31561 aaaaaattcc cgcaattacc ggcatgcatg cgggtgattt acagcccgat gtgatctttc
    31621 cccatgatcc actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat
    31681 ttcgttgtga atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca
    31741 cgcccatgaa acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta
    31801 aatgctcggt tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag
    31861 atctggtcat tctggaacat gccggaaccg tcattcgtac ttatggcgga gtggtgttga
    31921 ataaagagga atccgatccg cctatcgatc ataaaacgct catcaacacc cacaagaaag
    31981 agctgattgc agaagctgcc gttagtttta tccatgatgg cgattcgatc attcttgatg
    32041 ctggcagtac cgttttgcag atggttcccc tgctctcgcg ctttaataac atcacggtga
    32101 tgaccaacag cctgcatatc gtcaatgcgc tatccgaact ggataacgaa caaactatcc
    32161 tgatgccagg cggaacattt cgcaaaaaat cggcctcatt tcacgggcaa ctggcagaga
    32221 atgccttcga gcatttcacc ttcgataaat tgtttatggg caccgacggc atcgatctca
    32281 atgcgggcgt aaccacgttc aacgaggttt ataccgtcag taaggcaatg tgcaatgccg
    32341 cgcgcgaagt gattttgatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag
    32401 tttgcagtct tgaaagcgtc gataagctaa ttaccgacgc aggtatcgat ccggcgtttc
    32461 gtcaggcgct ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa
    32521 gcactactga acacgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta
    32581 ccggaacgtc tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc
    32641 aaagtggtgg tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca
    32701 cttgccagta ccggcacacc ggcttttttt gtccatccgg cagaagcact gcacggcgat
    32761 ctggggatga ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag
    32821 gaactggatc tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc
    32881 ggcaaaccga cgtcaccgct gggcctggcg gcgaaagcgg tgctggatat ctccgtagaa
    32941 cgcgaagcct gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg
    33001 ggtgacgcgc tggcgatggc ggtcatgcag gcgcgcggtt ttaatgaaga agattttgcc
    33061 cgctcccacc cagctggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg
    33121 cgtcgtgatg acgctatccc acaggtagcg ttaaccgcca gcgtgatgga tgcgatgctg
    33181 gaactcagcc gcaccggtct ggggctggtg gcggtatgtg acgctcaaca acaggtacaa
    33241 ggcgtcttta ccgacggcga tttacgtcgc tggctggtag gcggcggcgc actcaccacg
    33301 ccagttaatg aagcgatgac gacaggcggc accacattac aggcacaaag tcgcgccatc
    33361 gacgccaaag aagttctgat gaaacgcaaa atcactgccg caccagtggt agatgaaaac
    33421 ggcaaactca ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc
    33481 ttcaatccca gacgtttcgc cagccgatgc aggttggcga cgtcagtttc cagcattcgc
    33541 gcacaggctg cccagttgtg atgattttgt gccagtgcct ggcgaatagt ttcacgctgg
    33601 aacgcttctg tcgcttcacg caggttttgc ttaacaacga gcaccgccgc cgcttctggc
    33661 ggcggcaacg tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg
    33721 ttgcgggtgg ctctcgccag cactaccgcc cgatgaatag catgttccag ttcgcgcacg
    33781 tttcccggaa aactgtagtg ttgcagtaaa tttcgcgccc cggcacttaa taccacgcgg
    33841 gagagcccct gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca
    33901 tcgccccgct cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaaa
    33961 tcggcgcgga atcgccctgc cagcacctct tcgcgtaagt cgcggttagt cgccgccagc
    34021 acgcgcacat cgacccgcaa actacggtca tcgccaacgc gctgaatatc gccatactgc
    34081 aacaccctca gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atcgagaaac
    34141 agagtaccgt tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac
    34201 gctcctttca catgcccgaa caactcactt tccgccacac tttccggcag tgcggcacag
    34261 ttgagataga ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc
    34321 agctccttac cggttcccgt ctcaccgctg atcagaacgt tgagatcgga cgccgccaca
    34381 atctcaatct cttttttcag ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt
    34441 ttcaccgctt caaacggcgt ggcatcgcct ggcagcatat tctggctttc cagttgttca
    34501 atcagcaacg cattgcttaa cgctcccgcc gccagcgcgg caatcagccg taactcttcg
    34561 tcgctgaaaa catcgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg
    34621 ttttgtccga caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca
    34681 ggaatcaaac cgtcataggg atcgggcaat tcgctgtctg cgggaaagcg caccacatcc
    34741 ccggcgcggg caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt
    34801 acatcctttg ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc
    34861 aacgcagacg catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga
    34921 tcctgatgac caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa
    34981 aaactcatct ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat
    35041 tgatagtcat tttgactact cattaatgag cataatttta tttatagagt aaaaacaatc
    35101 agataagaaa ctggcacgca atctgcaatt agcaagacat ctttttagaa cacgctgaat
    35161 aaattgaggt tgctatgtct attgtggtga aaaataacat tcattgggtg ggtcaacgtg
    35221 actgggaagt gcgtgatttt cacggcacgg aatataaaac gctgcgcggc agcagctaca
    35281 acagctacct catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca
    35341 gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg
    35401 tgattaacca tgcagaagag gaccacgccg gggcgctgac cgaactgatg gcgcaaattc
    35461 ccgatacgcc gatctactgt actgccaacg ctatcgactc gataaatggt catcaccatc
    35521 atccggagtg gaattttaat gtggtgaaaa ctggcgacac gctggatatc ggcaacggca
    35581 aacagctcat ttttgtcgaa acaccaatgc tgcactggcc ggacagcatg atgacttacc
    35641 tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc
    35701 atctgttcaa cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg
    35761 ccaatatcct gacgccgttc agccgcctgg taacgccgaa aattaccgag atcctgggct
    35821 ttaacttacc agtcgatatg atagcgacct cccacggcgt ggtctggcgc gataacccga
    35881 cgcaaattgt cgagctgtac ctgaagtggg ccgctgatta tcaggaagac agaatcacca
    35941 ttttctacga caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggaa
    36001 ttgcggaaac cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa
    36061 acgaaatcct gaccaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga
    36121 ataacgtgat gatgccgaaa atcgccgggc tggtggagga gatgaccgga ttacgcttcc
    36181 gtaacaaacg cgccagtgct ttcggatctc acggctggag cggcggtgcg atggatcgcc
    36241 tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct cagcctgaaa gccaaatggc
    36301 gaccagacca ggacgctctg gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt
    36361 gggcgctcgc gccgctgccg cagagcacgg tgaatacggt agttaaagaa gaaacctctg
    36421 ccacgacgac ggctgacctc ggcccacgaa tgcagtgcag cgtctgccag tggatttacg
    36481 atccggcaaa aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc
    36541 cggataactt cctctgcccg gaatgctccc tcggcaaaga cgtctttgac gaactggcat
    36601 cggaggcaaa atgagcaacg gcattgtgat catcggttcg ggcttcgccg cccgccaact
    36661 ggtgaaaaat attcgcaaac aggacgccac tattccatta accctgattg ccgccgacag
    36721 catagatgag tacaacaaac ccgacctcag ccatgttatc agtcaggggc aacgtgccga
    36781 cgaccttacc cgccagacgg cgggtgaatt tgccgggcag tttaatctgc gcctgtttcc
    36841 gcacacctgg gtgacggata tcgatgccga agcccatgtg gtgaaaagcc agaataatca
    36901 gtggcaatac gacaagttag tgctggcaac cggtgccagc gcctttgtcc cgcctgtgcc
    36961 tgggcgtgag ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca
    37021 actgcgggat gcccgacgcg tgttgattgt cggcggtggt ttgattggta gcgaactggc
    37081 gatggatttt tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct
    37141 ggcgtcatta atgccaccag aagtaagcag ccgcttgcag catcggttga cggagatggg
    37201 cgttcatctg ctgttgaaat ctcagttgca ggggctggaa aaaacggatt ctggcattct
    37261 ggcaacgctg gaccaccagc gtagtatcga agtggatgcg gtaattgccg ccaccggact
    37321 gcgcccggaa acagccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt
    37381 cgatagttat ctgcaaacca gtaatgccga tatttacgcg ctgggcgatt gcgcggaaat
    37441 taacggtcag gtattgccgt tcctgcagcc gattcaactt agcgggatgg tgctggcaaa
    37501 aaatcttctc ggcaataaca cgccgctgaa actaccggcg atgctggtga aaatcaaaac
    37561 gccggaatta ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcagat
    37621 taataccgaa cgccagggaa tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc
    37681 ctttgtggtc agtgaggatc ggatgaaaga ggcatttgta ttgttgaaaa cgttgccggt
    37741 gtaggtgggc tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta
    37801 cggggacgca tgtgtaggcc ggataaggca tttacgccgc atccggcaag ggtgtccaaa
    37861 tgcaacacgc tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc
    37921 ccctgcccca gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg
    37981 aaatcagcga gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg
    38041 ctaaatacca gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa
    38101 ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat taaccagtgc ctgccagttc
    38161 agccactgct gccagaaagt ggcgagatcc agttgattgt ccacccgcgg catcgtcacc
    38221 ggatgcgtca ctccgtcgca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct
    38281 tcataactta acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacaaccgc
    38341 ccacacgatg atgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagtacgctc
    38401 cagttttgct gttgcacact tgccgtttca gggtaattct gccactccgg cacaaagcgc
    38461 aggcactgcg ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc
    38521 gccactgcag gcaagccgcc caggtgctca cattcgcgat agttcacccg caggcactcg
    38581 ccgccccaca aagcgccatt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca
    38641 tcaccgccat ccagcggcca ctgatgctct gccagacacg ccgctgcatg ggcatgatga
    38701 tgcagtaccg tttgcgtcgg cagattcatt tcgctcgccc actggctgga gacatagccc
    38761 ggatgcacat catgcacaat gtattgcgga gtgaaatcgt agatgttttg catcaggcgt
    38821 aacgcttcgc gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc
    38881 aacaccgctt gttcaccgcg caccaggcag aaggtatttt tcagatccgc gccaagacac
    38941 agcacaggcg gaacattttt aaagcccgga ggcaaaacca gcgcatccgg cacatacccc
    39001 cgcgaacggc gcagcatttc gccgctttcg cgcaccactg aatcatccat ccgctgcacg
    39061 atgtcgcggt tatgtagcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt
    39121 tcgttgctga tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat
    39181 tgcagttcct gtaacagcaa atgctggagc gggttcgcag gcaacatcac cccgacttcg
    39241 ttaaggccag gggcgatatc atcacaaagc tcaggtacgt attttttatc caccagcaca
    39301 atcggcgcag cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaact ttctgccact
    39361 ggcaacatga ctgccagcgg tttcgccggg cgatgtttgc gctcccgcag tgtcgccacc
    39421 gcgttactgt tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca
    39481 atgttgccca tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttctgcatgt
    39541 tcaccatgac ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg
    39601 tggaagcgac gatcgagcgg gtcgcggtac tctttgtcac aggccggaca tagcggaaac
    39661 gccgccatca cggtaaacgg gcggtcgtaa ggcatcgcgc gaataatggt gaaacgcggg
    39721 ccgcagtggg tacagttgat aaacggataa cgataacgcc gctcgcctgg ggtattcatt
    39781 tcggcaaggc aagcagggca agtggcggca tccgggacaa tttgcgtatt catggtgccg
    39841 ccggtgctct ggcggatggt gaactcggtg ggcagttgtg accagataaa cggctcacgc
    39901 tcgacgctat caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt
    39961 tccgggtctt ccagcagccg gacttctacg ccatcgccgt cattacatac atcgccgtga
    40021 agatttaatt gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg
    40081 ccacgaatac gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca
    40141 tcatgaattg cgtaacccgc cctgccggac acgacagcgt cgtatccggc agtctcaggt
    40201 cggcgatccc gcccgctccg tattctacga atatttccgg gaattccttt gatgccagaa
    40261 cagttctgta agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt
    40321 gttcaagttt attacgatcg acacaaatca gcgcatgggt cgggcaagcc gccatacacg
    40381 ccgggccgtc ttcacgatgg ttgcacaggt cgcatttatt ggcttcggct ttgtcagccc
    40441 gtacattcag acccgcgccg ctgttgcgaa tcacaggacg taccaccact tccatcgcgc
    40501 catacgggca agccaccacg caggttttgc aaccaatgca acgttcctgc atcacaggaa
    40561 caaacccttt atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt
    40621 cacactgacg gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg
    40681 gtaaaaatgt ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc
    40741 acgccacttc acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt
    40801 tcatcaaatt ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt
    40861 tttaatttgc tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg
    40921 acactagtga cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg
    40981 cggcggatca gttttccgct aaaggttttc ggcggtgaga aatccccgcc atcgagcata
    41041 aaaatcagcc gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag
    41101 agcgccggaa cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc
    41161 tctggcaccg ctacgccgcg ctcgtgcaac gctttgatcg cacctatcgc catatcgtcg
    41221 ttactggcaa ctaacgcgct aaatttagcc cctcgttcga gcaacgtttc taccccttcg
    41281 gccccgctgg cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca
    41341 tgctgcgaca gcgcgtcttt atacccggca agacgttcaa tgctggtggg ggaatccatc
    41401 gagccggtaa ggaaagcaat ctcctgatgc ccggcgttta tcaactctgc cacggcgtta
    41461 aagctggtct gtttatgatc gcaccagacg ctatggctgc tattattgcg caggcggcga
    41521 ttaagcacca ttatcggctg actgtgcgcg tcaatgatgt catcaatctc atccacgctt
    41581 aaaaaacgcg ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc
    41641 tggcgctctt cttcggcgct gtgtttacca tctgccaata gcaactgtcg ccctttctct
    41701 tccgccattc gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg ataaagcgtg
    41761 ttggtcacta ccagccccag cgtctgagta ctcttcgccg acagattgcg ggccagcaaa
    41821 tttgggcggt aactgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg
    41881 acgtagccat tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc
    41941 gccacttcca gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac
    42001 tgcaccgttt gccgattgtc cttgcgcaat cggctccagc ggataagacg aggtcgccgt
    42061 gccggttttg atgacttttt tgataaaggt aaacatggcg attccttatt tcagcggcga
    42121 gtttttacgc tcaatgctgt aacgctcgag ttctttgtac ggcaccactt tgctcttctt
    42181 cttacgcaca tcgactacgg tcatgcggtc ggtacaggag tagcaagggt ccaggctacc
    42241 gataatcagc ggcgcatcgg aaacggtatt gccacgcagc atgtagcgca gggtcggcca
    42301 gttcgcgtag gtcgcggcac ggcagcgcca gcggtacagc ttctggttgt cgccggtcat
    42361 gctccagtgg atatcgtcgc cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg
    42421 aatgtaggta aagccttcca ccatcagcgg accgcccggc aggttatcca gaccgtagtc
    42481 gatcatattc agcgcggtat agacctcgtt gattcgcact ttcagacggg aaataacgtc
    42541 gcagccctgc tcgctgtgga cttccattgg cagcaggcca taaccgacaa acgggtgatc
    42601 ggcgcgagta tcgcgggcga gaccgctggc gcgaaccatc gggccaacgt tactgaaatc
    42661 gcgggcgatt tccgggtcca gacgaccaat gccgacggtg cgttgttcca tgttcggcgt
    42721 actcagcagc acatccacca gctcctgcac ttcacgacgc atttgttgcg ccagctggcg
    42781 ggtctggatc atgtcatctt tcagcagatc gcgacgaatc ccgccgatca ggttcaggcc
    42841 gtaggtttta cgcgcaccgg taaggatctc tgccattttc atggaggttt cacgcacgcg
    42901 gaagaactgc ataaagccgg agtcgaagcc ggtaaagtga caggcgaggc cgaggttgag
    42961 cagatgcggg tgcaggcgtt ccacctccag cagaatggcg cggatcatct gcgcgcgttc
    43021 tggcacctga atacccatcg cgttttccac cgacgtggtg taggcggtgc tgtgggcaaa
    43081 gccgcagatc ccgcacacac ggtcagagag gaaggtcact tcgttataac ccatgcgggt
    43141 ttccgccagt ttttccatac cgcgatggac gtagaacaga cggtagtcgg cgtcgataat
    43201 gttttcgcca tcgacgaaca gacggaagtg acccggttcg tcagaagtga cgtgcagcgg
    43261 accaatcggc acgacgttgt ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc
    43321 agcatcggta gtcggtgccg gacgctgacg ataatccatg ctgtctttac gcagcggata
    43381 aagttcatcc ggccagtcat ccggcagcac cagacgacgt tcatccggca gaccaaccgg
    43441 aatcaaaccg tacatatcgc gcacttcgcg ctcgccccac accgccgccg gaacgcgcgg
    43501 cgtcacggac ggatactccg gtttgttggc gtcaacttcg acgcgaaccg ttacccaaca
    43561 cttggtgccc ttctccatcg acagcacgta ataaacggcg taatgaccat tcagtttgcg
    43621 ttcgtcgtta ccaaacagca ccgacagcca gccaccctgc ttgtagtaga gaaactccac
    43681 cacttccggc agatagttca cctttacggt gacagtcagc tgatctttgg tctgccaggc
    43741 gtggtccagc acgacgccta gaaatgcctc attcagcgcg gcgagataat gttgacctaa
    43801 tttttcttca gacatgctca aactctcttt aatcacgccg ccagcaagga gacgaacgct
    43861 aaaaatgcaa agccaaaccc agcccaggta atgcgcggag taatatcaag acgcagacgg
    43921 gccatgctgt tttcgaacag cgcgataacc aggacgccga ccaccagttt gacgatggca
    43981 atcaccagcg ccagcagcag tccaccgacg gtgaaggttt ccatttgtcc ccacggaata
    44041 aacaccccga cgaacatctg caacaccacc agctgtttca ggctgatacc ccatttcatg
    44101 acgccaaagc cgctaccgct gtattcggag agcggacctt cctgcaactc ctgctcggct
    44161 tccgccaggt cgaacggcag tttgcccatt tcgataaaag tggcgaacgc acaggcgcag
    44221 agcgccagca ccagcgggat gctttggctc agcggccagt gataaacggt gtcggtaatg
    44281 ttgctgatgt tggtggaacc ggcaacctgt gcggcaaccc acagaccaag cagcaacatc
    44341 ggttcgacca gcacgccaag catcgcttca cggctcgcac cgatagcggt aaacgggcta
    44401 ccggtatcca gaccggaaat ggcaaagaag aaacgcgcga tggcgaagag atacagtaag
    44461 gtgatcaaat cgcccagttg cggcagcgga gaaccgacgg tcaccaccgg cagcgcagta
    44521 gcgatagtca gcatgacgcc caccatcaca tacggcgtca ggcggaacat ccagccagag
    44581 gcatccgggc caacgctctg gcggcccagc agtttgataa tgtcgcgata ctcctgcaac
    44641 acgcccggcc cgcgacggtt atgcagacgg gcgcgcgcca cgcgggtaat accggagagc
    44701 agcggcgcaa cggcaaataa caccagcgcc tgaattaacg gatataaaac actcattctc
    44761 aggctcctcg tgaaacaata atcaccacca gtaccgccag ttcaaccagc gccatccggc
    44821 ggaacagcaa cgcactacct tcgcactgcc agcccggcac tagcgacacc ggattcagcc
    44881 atttgcgcag tttcagcact ggcgcaaacg cctgtttaac cggcatggca aaaccgtgag
    44941 cggtaatcac cattgatttt tcatggtcgt agccgcacac ccaggccgca ccgcgggaac
    45001 gtgacggcag gcggtcgcct ttgcaaatcg ccataatgat gaatggcagc agcgggcagg
    45061 caatcagcag caatgtgatc attggttgag aaacggtggt gttagcaggc tccagaggca
    45121 gaggtacagc agcagagagc atcggcagca gccacggcgc agcaacaccg ccaattacgc
    45181 agcaaatcgc cagtgccact acgcttacgc tcatcaggag cggcgcacag gtggcgtttt
    45241 cggcttcttt ggtacgcggc gcgccgagga acgtgacgcc atagactttc gccatacaca
    45301 tcaccgccag cgcaccggta attgccagac ccacagcgag cagcggcccc agcagacggg
    45361 caacaaacgc gccgctattg ctcagtttga agaaggattg atagataacc cattccccgg
    45421 caaacccgtt cagtggcggc agcgcagcca ttgccatcaa cccgactaac atggcgatgg
    45481 agataaccgg cattttcttg ccaataccac cgagtttttc gatatcgcga tgaccggtac
    45541 ggaaccagac gctccccgcc ccgaggaaca gtacgctttt gaacaggcta tggttaagca
    45601 gatggtacag accaccgacc aggccaagag caatcagcgc cggttgttcg agcgcgatac
    45661 ccgttacgcc agcgcccagc cccagcccca gccccagcag gataatgccg atattttcca
    45721 gggtgtggta agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga
    45781 cgaacgcggt gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac
    45841 cgcccagcag tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa
    45901 acaacgcggc agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg
    45961 gaataatccc agccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga
    46021 gcggcagctg ttgcatacgc atatccagca ggcgcagatc cagcgtgcca taacgctgcc
    46081 acagcagcca gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca
    46141 gtttgccctc tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca
    46201 ttaccacgaa catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca
    46261 tattgatctg caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttataga
    46321 ggctgacaaa cagaccgcaa aggccgaggg taatcagcca aatcgcgtta agcggggaga
    46381 tttgcacatc gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta
    46441 cagtgaagcc cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccggctatcc
    46501 agccacttag cgctttttga aaagagaaat gccagaacag cggcggcgac aaaccatgcc
    46561 acgccgctat tgatcaggga aattgcgctc atttagcctc tccactttga gcctgctgaa
    46621 acaaggtgag atcgccaaag tccgtgttaa aggtcagctc acgcttacgt ttgctgacgc
    46681 gggcgatatc ggtgttatcc accagatgca gggctttagt cgggcacatc cgcacacagg
    46741 ccggaccttg ttcatcaaag ctacaaaggt cacatttgac ggcgatcgcg cgaatacctg
    46801 gcacccagtc aagcaatgtg ctgacacgcg ccggagcagg cggtgccggt ggcgctttcg
    46861 gggtattggc gtttgccgga atatccagcg gacggctgcc ggaaaattca attgcgccaa
    46921 acgggcaggc gatgccgcac agtttgcagc ttacgcacag actttcattc aactgcacgg
    46981 ccccatcaac gcgggtgatg gcgttaaccg ggcagaccac cgcgcagggt gcatcttcac
    47041 agtggtgaca gagctgcggc gcagattctt tttcattgag catcactctc aggcgcggca
    47101 ttgattgcag gccgtgctgg cgatgcgtct ctgaacaggc ggcctcacag gtgtggcagc
    47161 cgatacagag cgtggagtcg gcaattacaa aacgattcac caggcattcc tcaggtgatt
    47221 gtcatttttg acgaaaacat gccgatgaaa tgtcattttc gacactcatc gacacgccca
    47281 tccccaaaca ggcgtaacgc ctgcaaaacg ggcaaagcct cagctcatgc tgccgggctt
    47341 tgtcccttta ccagttggct taaattcacc ggcacattgt tttcaatggc ggtgtataag
    47401 ctgtcgtaaa cgccagcgat tttttcgaga tagtgttcca gaacctgttc gcgatcgcgg
    47461 atgttgacct tgccatcaat catgccgtca atgctcagtt gcgcctgcgc aatccgctgt
    47521 ttgtcttcgc catcttttgt ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc
    47581 gccagggtga cgtaaatacg aaaatgttca aaaaggacga aacagagttc tttgtccggc
    47641 gcgcagctca tggcgtgata caggcgcgct ttcgataacg tgatcccctg aaccagcgaa
    47701 ttgcagtaga tgtgccactg gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct
    47761 ttctcgctta tttcccaaat agtcattgtc aggttactcg tttaacagag atgccagctt
    47821 taagcatttt ttgtgccaac ttttaattta ttgttattaa agagattttt aagctaaaga
    47881 tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat cgacattatt caccgcaggg
    47941 ataatcaaca ctggcgcaat tattgcttgt agctggcaat agttaatggg aggcgatatg
    48001 cacgaaataa ccctctgcca acgggcactg gaattgatcg aacagcaggc cgcaaaacac
    48061 ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg cattttcttg tgtcgaaacc
    48121 agctctcttg ccttttgttt tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa
    48181 ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg
    48241 ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg gtgacatgct acagattgtg
    48301 gcagacgacg gtttacagat tcggcggata gaaatagacc aggagtgagc gatgtgtaca
    48361 acatgcggtt gcggtgaagg caacctgtat atcgagggtg atgaacataa ccctcattcc
    48421 gcgtttcgta gcgcgccatt tgccccggcg gcacgcccgc agatgaaaat caccggcatt
    48481 aaagcgcctg aatttacccc cagccagact gaagaaggcg acctgcatta cggtcatggc
    48541 gaagcgggca ctcacacacc gggcatgagc cagcgtcgga tgctggaagt cgaaattgac
    48601 gtgctggaca aaaacaaccg tctggctgaa cgcaaccgcg cgcgctttgc tgcccgcaag
    48661 caactggtgc tcaacctggt ttccagccct ggttccggta aaaccaccct gctgacggaa
    48721 accttaatgc gcctgaaaga cagcgttccg tgcgcggtta ttgaaggcga ccagcaaacc
    48781 gtgaacgatg ccgcacgcat tcgcgctacc ggtacaccag cgattcaggt gaacaccggt
    48841 aaaggctgcc atcttgacgc acagatgatt gccgacgccg caccgcgtct gccactggac
    48901 gataacggta ttctgtttat cgaaaacgtt ggcaacctcg tatgcccggc cagcttcgat
    48961 ctcggtgaaa aacacaaagt agcggtgctt tccgttaccg aaggtgaaga caaaccgctg
    49021 aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc tcaacaaagt tgacctgttg
    49081 ccgtatctca actttgacgt tgagaagtgc atcgcctgcg cccgcgaagt caatccagaa
    49141 attgaaatca tccttatttc cgccaccagc ggcgaaggga tggaccagtg gctgaactgg
    49201 ctggagacac agcgatgtgc ataggcgttc ccggccagat ccgcaccatt gacggcaacc
    49261 aggcgaaagt cgacgtctgc ggcattcagc gcgatgtcga tttaacgtta gtcggcagct
    49321 gcgatgaaaa cggtcagccg cgcgtgggcc agtgggtact ggttcacgtt ggctttgcca
    49381 tgagcgtaat taatgaagcc gaagcacgcg acactctcga cgccttacaa aacatgtttg
    49441 acgttgagcc ggatgtcggc gcgctgttgt atggcgagga aaaataatgc gttttgttga
    49501 tgaatatcgc gcgccggaac aggtgatgca gttaattgag catctgcgcg aacgtgcttc
    49561 acatctctct tacaccgccg aacgccctct gcggattatg gaagtgtgtg gcggtcatac
    49621 ccacgccatt tttaaattcg gcctcgacca gttactgcct gaaaacgttg agtttatcca
    49681 cggtccgggt tgcccggtgt gcgtactgcc gatgggtaga atcgacacct gcgtggagat
    49741 tgccagccat ccggaagtca tcttctgtac ctttggcgac gccatgcgcg taccagggaa
    49801 acagggatcg ctgttgcagg caaaagcacg cggtgccgat gtgcgcatcg tttactcgcc
    49861 gatggatgcg ttgaaactgg cgcaggagaa tccaacccgc aaagtggtgt tcttcggctt
    49921 aggttttgaa accaccatgc cgaccaccgc catcactctg caacaggcga aagcgcgtga
    49981 tgtgcagaat ttttacttct tctgccagca cattacgctt atcccgacgc tgcgcagttt
    50041 gctggaacag ccggataacg gtatcgacgc gttcctcgcg ccgggccacg ttagtatggt
    50101 tatcggcact gatgcctata attttatcgc cagcgatttt catcgtccgc tggtggtcgc
    50161 tggtttcgaa ccccttgatc tactgcaagg cgtggtcatg ctggtggagc agaaaatagc
    50221 ggcccacagc aaggtagaga atcagtatcg tcgggtggta ccggatgccg gtaacctgct
    50281 ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc gacagcgaat ggcgcggctt
    50341 aggcgtgatt gaatcttctg gcgtgcacct gacgccggat tatcaacgat tcgatgccga
    50401 agcacatttc cgcccggcac cgcagcaggt ctgcgatgac ccgcgcgcgc gttgtggtga
    50461 ggtattaacg ggcaaatgta agccgcatca atgcccgctg tttggtaaca cctgtaatcc
    50521 tcaaaccgca tttggtgcac tgatggtctc ctccgaagga gcgtgcgccg cgtggtatca
    50581 gtatcgtcag caggagagtg aagcgtgaat aatatccaac tcgcccacgg cagcggcggc
    50641 caggcgatgc agcaattaat caacagcctg tttatggaag cctttgccaa cccgtggctg
    50701 gcagagcagg aagatcaggc acgtcttgat ctggcgcagc tggtagcgga aggcgaccgt
    50761 ctggcgttct ccaccgacag ttacgtcatt gacccgctgt tctttcctgg cggtaatatc
    50821 ggcaagctgg cgatttgcgg caccgcgaat gacgttgcgg gcagtggcgc tattccgcgc
    50881 tatctctcct gtggctttat cctcgaagaa ggattgccga tggagacact gaaagccgta
    50941 gtgaccagca tggcagaaac cgcccgcacg gcaggcattg ccatcgttac tggcgatact
    51001 aaagtggtgc agcgcggcgc ggcagataaa ctgtttatca acaccgctgg catgggcgca
    51061 attccggcga atattcactg gggcgcacag acgctaaccg caggcgatgt attgctggtt
    51121 agcggtacac tcggcgacca cggggcgact atccttaacc tgcgtgagca gctggggctg
    51181 gatggcgaac tggtcagcga ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac
    51241 attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat
    51301 gagttcgcgg cagcctgcgg ttgtggtatt gaaatttctg aatcagcgct gccggttaaa
    51361 cctgccgtgc gcggcgtttg cgaattgctg ggactggacg ccctgaactt tgccaacgaa
    51421 ggcaaactgg tgatcgccgt tgaacgcaac gcggcagagc aagtgctggc agcgttacat
    51481 tcccatccac tggggaaaga cgcggcgctg attggtgaag tggtggaacg taaaggtgtt
    51541 cgtcttgccg gtctgtatgg cgtgaaacga accctcgatt taccacacgc cgaaccgctt
    51601 ccgcgtatat gctaataaaa ttctaaatct cctatagtta gtcaatgacc ttttgcaccg
    51661 ctttgcggtg ctttcctgga agaacaaaat gtcatataca ccgatgagtg atctcggaca
    51721 gcaagggttg ttcgacatca ctcggacact attgcagcag cccgatctgg cctcgctgtg
    51781 tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt
    51841 gtggcaagcg cagactcaac gtgcgtctta ttacgcgtcg cgtgaaaaag acacccccat
    51901 taaatatgaa gacgaaactg ttctggcaca cggtccggta cgcagcattt tgtcgcgccc
    51961 tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc tggccgcagc tggccgcagg
    52021 tgggctatac cccaaatttg gtcactattg cctgatgcca ctggcggcgg gagggcatat
    52081 ttttggtggc tgtgaattta ttcgttatga cgagcgtccc tggagcgaaa aagagttcaa
    52141 tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc gaacaaatcc agagccgcgt
    52201 cgtcaacaat gtcgactatg agttgttatg ccgggagcgc gataacttcc gcatcctggt
    52261 tgccattacc aacgcggtgc tttcccgcct ggatatggac gaactggtca gcgaagtcgc
    52321 caaagaaatc cattactatt tcgacattga cgatatcagc attgtcttac gcagccaccg
    52381 taaaaacaaa ctcaacatct actccactca ctatcttgat aaacaacatc ccgcccacga
    52441 acagagcgaa gtcgatgaag ccggaaccct caccgaacgc gtgttcaaaa gtaaagagat
    52501 gctgctgatc aatctctacg agcgggacga tttagccccc tatgaacgca tgttgttcga
    52561 cacctggggc aaccagattc aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat
    52621 gctgggcgtg ctgaaactgg cgcaatgcga agagaaagtg tttaccacta ccaatctgaa
    52681 tttgctgcgc cagattgccg aacgtgtggc aatcgctgtc gataacgccc tcgcctatca
    52741 ggaaatccat cgtctgaaag aacggctggt tgatgaaaac ctcgccctga ccgagcagct
    52801 caacaatgtt gatagtgaat ttggcgagat tattggccgc agcgaagcca tgtacagcgt
    52861 gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc gtgctgatcc tcggtgaaac
    52921 tggctcgggt aaagagctga ttgcccgtgc gatccataat ctcagtgggc gtaataatcg
    52981 ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga ttgctggaaa gcgatctgtt
    53041 tggtcatgag cgtggggctt ttaccggtgc cagcgcccag cgcattggtc gttttgaact
    53101 ggcggataaa agctctctgt tcctcgacga agtgggcgat atgccactgg agttacagcc
    53161 gaagttgctg cgtgtattgc aggaacagga gtttgaacgt ctcggcagca acaaaatcat
    53221 tcagacggac gtacgtttaa ttgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga
    53281 ccgagagttc cgtagcgatc tctattaccg cctgaacgta ttcccgattc acctgccgcc
    53341 actacgcgag cgtccggaag atattccgct gctggcgaaa gcctttacct tcaaaattgc
    53401 ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag acgctgcgca ccttgagcaa
    53461 catggagtgg ccgggtaacg tacgcgaact ggaaaacgtc attgagcgcg cagtattgct
    53521 aacacgcggc aacgtgctgc agctgtcatt gccagatatt gctttaccgg agcctgaaac
    53581 gccgcctgcc gcaacggttg tcgcccagga gggcgaagat gaatatcagt tgattgtgcg
    53641 cgtgctgaaa gaaaccaaca gcgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg
    53701 gctgaaacgc acgaccctgc tgtcacggat gaagcggctg ggaattgata aatcggcatt
    53761 gatttaactg caaattgccg gacagatctg cctgtccggc atactattca cgaggttttt
    53821 tcggacgata tttttccggc agttctggca ccggacgctt gtcatcgatg agatgacgca
    53881 cggttaagat cggatgacgc cacagcattc tcggcccggc ccaacgcata atctgtttca
    53941 tctcttcacg ctttgcaggc tggtaacagt gcaccggaca ctgcttacag gctggtttct
    54001 cttcgccgaa cacacattta tccagccgct tttgcgcgta aacaaacaac gcctcgtaat
    54061 gctccggctc cgctgacgcc tgcgggcatt tcgcttgata aagatcgatc atttttttaa
    54121 tcgtcagttt ttcacgagag atacgcttgc cggacatgct gcctccacct cattaagatg
    54181 catttatatt acatcttaat
//
LOCUS       DAEBXD010000015        53213 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEBXD010000015 DAEBXD010000000
VERSION     DAEBXD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53213
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.015"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(11..322)
                     /locus_tag="KJL94_000909"
     CDS             complement(11..322)
                     /locus_tag="KJL94_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBD4409372.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="KJL94_000910"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KJL94_000910"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="KJL94_000911"
     CDS             701..1054
                     /locus_tag="KJL94_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBD4409373.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KJL94_000912"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KJL94_000912"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBD4409374.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="KJL94_000913"
     CDS             complement(2810..3280)
                     /locus_tag="KJL94_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBD4409375.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KJL94_000914"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KJL94_000914"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBD4409376.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KJL94_000915"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KJL94_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD4409377.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KJL94_000916"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KJL94_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBD4409378.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="KJL94_000917"
     CDS             complement(5283..8432)
                     /locus_tag="KJL94_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBD4409379.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KJL94_000918"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KJL94_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBD4409380.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="KJL94_000919"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="KJL94_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBD4409381.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="KJL94_000920"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="KJL94_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBD4409382.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KJL94_000921"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KJL94_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBD4409383.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KJL94_000922"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KJL94_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBD4409384.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="KJL94_000923"
     CDS             14911..15288
                     /locus_tag="KJL94_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBD4409385.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="KJL94_000924"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="KJL94_000924"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBD4409386.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="KJL94_000925"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="KJL94_000925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBD4409387.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="KJL94_000925"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="KJL94_000926"
     CDS             18099..18428
                     /locus_tag="KJL94_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBD4409388.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="KJL94_000927"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="KJL94_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBD4409389.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="KJL94_000928"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="KJL94_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBD4409390.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="KJL94_000929"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="KJL94_000929"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBD4409391.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="KJL94_000930"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="KJL94_000930"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBD4409392.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KJL94_000931"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KJL94_000931"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBD4409393.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="KJL94_000932"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="KJL94_000932"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBD4409394.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="KJL94_000933"
     CDS             complement(25481..27157)
                     /locus_tag="KJL94_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBD4409395.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KJL94_000934"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KJL94_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBD4409396.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="KJL94_000935"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="KJL94_000935"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD4409397.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KJL94_000936"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KJL94_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBD4409398.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="KJL94_000937"
     CDS             complement(31205..31999)
                     /locus_tag="KJL94_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBD4409399.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="KJL94_000938"
     CDS             32137..32478
                     /locus_tag="KJL94_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD4409400.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KJL94_000939"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KJL94_000939"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBD4409401.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="KJL94_000940"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="KJL94_000940"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBD4409402.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="KJL94_000941"
     CDS             36394..37686
                     /locus_tag="KJL94_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4409403.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="KJL94_000942"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="KJL94_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBD4409404.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="KJL94_000943"
     CDS             complement(38219..38677)
                     /locus_tag="KJL94_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBD4409405.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="KJL94_000944"
     CDS             complement(38674..39591)
                     /locus_tag="KJL94_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD4409406.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="KJL94_000945"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="KJL94_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBD4409407.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="KJL94_000946"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="KJL94_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBD4409408.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KJL94_000947"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KJL94_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBD4409409.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KJL94_000948"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KJL94_000948"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD4409410.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KJL94_000949"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KJL94_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="KJL94_000950"
     CDS             43550..44236
                     /locus_tag="KJL94_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4409411.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="KJL94_000951"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="KJL94_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="KJL94_000952"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="KJL94_000952"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBD4409412.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="KJL94_000953"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="KJL94_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBD4409413.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVTPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="KJL94_000954"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="KJL94_000954"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBD4409414.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="KJL94_000955"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="KJL94_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBD4409415.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="KJL94_000956"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="KJL94_000956"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBD4409416.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53213
                     /locus_tag="KJL94_000957"
     CDS             52938..>53213
                     /locus_tag="KJL94_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBD4409417.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIR"
BASE COUNT        12828 a        13690 c        14023 g        12672 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acattggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg gttacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgt
//
LOCUS       DAEBXD010000016        52077 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEBXD010000016 DAEBXD010000000
VERSION     DAEBXD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52077
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.016"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KJL94_000958"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KJL94_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBD4409418.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="KJL94_000959"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="KJL94_000959"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD4409419.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="KJL94_000960"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="KJL94_000960"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD4409420.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="KJL94_000961"
     CDS             complement(5906..6949)
                     /locus_tag="KJL94_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBD4409421.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KJL94_000962"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KJL94_000962"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBD4409422.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KJL94_000963"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KJL94_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBD4409423.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="KJL94_000964"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="KJL94_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="KJL94_000965"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="KJL94_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBD4409424.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KJL94_000966"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KJL94_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBD4409425.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="KJL94_000967"
     CDS             15474..15830
                     /locus_tag="KJL94_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBD4409426.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="KJL94_000968"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="KJL94_000968"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBD4409427.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="KJL94_000969"
     CDS             16989..17189
                     /locus_tag="KJL94_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBD4409428.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KJL94_000970"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KJL94_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD4409429.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KJL94_000971"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KJL94_000971"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBD4409430.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="KJL94_000972"
     CDS             complement(20101..20964)
                     /locus_tag="KJL94_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBD4409431.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KJL94_000973"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KJL94_000973"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBD4409432.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KJL94_000974"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KJL94_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBD4409433.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDVGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KJL94_000975"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KJL94_000975"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBD4409434.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="KJL94_000976"
     CDS             24133..24705
                     /locus_tag="KJL94_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBD4409435.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="KJL94_000977"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="KJL94_000977"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBD4409436.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="KJL94_000978"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="KJL94_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBD4409437.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="KJL94_000979"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="KJL94_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="KJL94_000980"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="KJL94_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBD4409438.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="KJL94_000981"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="KJL94_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBD4409439.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="KJL94_000982"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="KJL94_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBD4409440.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="KJL94_000983"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="KJL94_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBD4409441.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="KJL94_000984"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="KJL94_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="KJL94_000985"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="KJL94_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBD4409442.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="KJL94_000986"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="KJL94_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBD4409443.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="KJL94_000987"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="KJL94_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBD4409444.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="KJL94_000988"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="KJL94_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBD4409445.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="KJL94_000989"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="KJL94_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="KJL94_000990"
     CDS             complement(39099..40160)
                     /locus_tag="KJL94_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD4409446.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="KJL94_000991"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="KJL94_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBD4409447.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="KJL94_000992"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="KJL94_000992"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBD4409448.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KJL94_000993"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KJL94_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBD4409449.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KJL94_000994"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KJL94_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBD4409450.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KJL94_000995"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KJL94_000995"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBD4409451.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="KJL94_000996"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="KJL94_000996"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBD4409452.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="KJL94_000997"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="KJL94_000997"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBD4409453.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVQAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="KJL94_000998"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="KJL94_000998"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBD4409454.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="KJL94_000999"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="KJL94_000999"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBD4409455.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52077)
                     /gene="pdeF"
                     /locus_tag="KJL94_001000"
     CDS             complement(50969..>52077)
                     /gene="pdeF"
                     /locus_tag="KJL94_001000"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD4409456.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12158 a        13738 c        13520 g        12661 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 caacatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacag gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgt
//
LOCUS       DAEBXD010000017        50305 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEBXD010000017 DAEBXD010000000
VERSION     DAEBXD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50305
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.017"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(435..641)
                     /locus_tag="KJL94_001001"
     CDS             complement(435..641)
                     /locus_tag="KJL94_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBD4409457.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="KJL94_001002"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="KJL94_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBD4409458.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="KJL94_001003"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="KJL94_001003"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBD4409459.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="KJL94_001004"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="KJL94_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="KJL94_001005"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="KJL94_001005"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBD4409460.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="KJL94_001006"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="KJL94_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="KJL94_001007"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="KJL94_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBD4409461.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="KJL94_001008"
     CDS             8989..9093
                     /locus_tag="KJL94_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBD4409462.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11592
                     /gene="speF"
                     /locus_tag="KJL94_001009"
                     /pseudo
     CDS             9404..11592
                     /gene="speF"
                     /locus_tag="KJL94_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11589..12908
                     /gene="potE"
                     /locus_tag="KJL94_001010"
     CDS             11589..12908
                     /gene="potE"
                     /locus_tag="KJL94_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBD4409463.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12964..14604)
                     /gene="pgm"
                     /locus_tag="KJL94_001011"
     CDS             complement(12964..14604)
                     /gene="pgm"
                     /locus_tag="KJL94_001011"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBD4409464.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14630..15175)
                     /gene="seqA"
                     /locus_tag="KJL94_001012"
     CDS             complement(14630..15175)
                     /gene="seqA"
                     /locus_tag="KJL94_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBD4409465.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15360..16124
                     /gene="ybfF"
                     /locus_tag="KJL94_001013"
     CDS             15360..16124
                     /gene="ybfF"
                     /locus_tag="KJL94_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD4409466.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16264..16557
                     /gene="ybfE"
                     /locus_tag="KJL94_001014"
     CDS             16264..16557
                     /gene="ybfE"
                     /locus_tag="KJL94_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBD4409467.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16697..17227
                     /gene="fldA"
                     /locus_tag="KJL94_001015"
     CDS             16697..17227
                     /gene="fldA"
                     /locus_tag="KJL94_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBD4409468.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17437..17523
                     /gene="uof"
                     /locus_tag="KJL94_001016"
     CDS             17437..17523
                     /gene="uof"
                     /locus_tag="KJL94_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBD4409469.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17516..17962
                     /gene="fur"
                     /locus_tag="KJL94_001017"
     CDS             17516..17962
                     /gene="fur"
                     /locus_tag="KJL94_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBD4409470.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18046..18372)
                     /locus_tag="KJL94_001018"
     CDS             complement(18046..18372)
                     /locus_tag="KJL94_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409471.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="KJL94_001019"
     CDS             complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="KJL94_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBD4409472.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20394..22058)
                     /gene="glnS"
                     /locus_tag="KJL94_001020"
     CDS             complement(20394..22058)
                     /gene="glnS"
                     /locus_tag="KJL94_001020"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBD4409473.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22261..24207)
                     /gene="nagE"
                     /locus_tag="KJL94_001021"
     CDS             complement(22261..24207)
                     /gene="nagE"
                     /locus_tag="KJL94_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBD4409474.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24540..25340
                     /gene="nagB"
                     /locus_tag="KJL94_001022"
     CDS             24540..25340
                     /gene="nagB"
                     /locus_tag="KJL94_001022"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD4409475.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRLFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25400..26548
                     /gene="nagA"
                     /locus_tag="KJL94_001023"
     CDS             25400..26548
                     /gene="nagA"
                     /locus_tag="KJL94_001023"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD4409476.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26557..27777
                     /gene="nagC"
                     /locus_tag="KJL94_001024"
                     /gene_synonym="nagR"
     CDS             26557..27777
                     /gene="nagC"
                     /locus_tag="KJL94_001024"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBD4409477.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27825..28577
                     /gene="nagD"
                     /locus_tag="KJL94_001025"
     CDS             27825..28577
                     /gene="nagD"
                     /locus_tag="KJL94_001025"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBD4409478.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28974..30638
                     /gene="asnB"
                     /locus_tag="KJL94_001026"
     CDS             28974..30638
                     /gene="asnB"
                     /locus_tag="KJL94_001026"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBD4409479.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31017..31093
                     /locus_tag="KJL94_001027"
     tRNA            31017..31093
                     /locus_tag="KJL94_001027"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31051..31053,aa:Met,seq:cat)
     gene            31102..31186
                     /locus_tag="KJL94_001028"
     tRNA            31102..31186
                     /locus_tag="KJL94_001028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31136..31138,aa:Leu,seq:tag)
     gene            31210..31284
                     /locus_tag="KJL94_001029"
     tRNA            31210..31284
                     /locus_tag="KJL94_001029"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31242..31244,aa:Gln,seq:ttg)
     gene            31319..31393
                     /locus_tag="KJL94_001030"
     tRNA            31319..31393
                     /locus_tag="KJL94_001030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31351..31353,aa:Gln,seq:ttg)
     gene            31409..31485
                     /locus_tag="KJL94_001031"
     tRNA            31409..31485
                     /locus_tag="KJL94_001031"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31443..31445,aa:Met,seq:cat)
     gene            31534..31608
                     /locus_tag="KJL94_001032"
     tRNA            31534..31608
                     /locus_tag="KJL94_001032"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31566..31568,aa:Gln,seq:ctg)
     gene            31646..31720
                     /locus_tag="KJL94_001033"
     tRNA            31646..31720
                     /locus_tag="KJL94_001033"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31678..31680,aa:Gln,seq:ctg)
     gene            complement(31874..33049)
                     /gene="ubiF"
                     /locus_tag="KJL94_001034"
     CDS             complement(31874..33049)
                     /gene="ubiF"
                     /locus_tag="KJL94_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBD4409480.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33195..34619
                     /gene="miaB"
                     /locus_tag="KJL94_001035"
     CDS             33195..34619
                     /gene="miaB"
                     /locus_tag="KJL94_001035"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBD4409481.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34772..35812
                     /locus_tag="KJL94_001036"
     CDS             34772..35812
                     /locus_tag="KJL94_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBD4409482.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35809..36276
                     /gene="ybeY"
                     /locus_tag="KJL94_001037"
     CDS             35809..36276
                     /gene="ybeY"
                     /locus_tag="KJL94_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBD4409483.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36366..37244
                     /gene="corC"
                     /locus_tag="KJL94_001038"
     CDS             36366..37244
                     /gene="corC"
                     /locus_tag="KJL94_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBD4409484.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37269..38807
                     /gene="lnt"
                     /locus_tag="KJL94_001039"
     CDS             37269..38807
                     /gene="lnt"
                     /locus_tag="KJL94_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBD4409485.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39204..40112
                     /gene="gltI"
                     /locus_tag="KJL94_001040"
     CDS             39204..40112
                     /gene="gltI"
                     /locus_tag="KJL94_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBD4409486.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40282..41022
                     /gene="gltJ"
                     /locus_tag="KJL94_001041"
     CDS             40282..41022
                     /gene="gltJ"
                     /locus_tag="KJL94_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBD4409487.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41022..41696
                     /gene="gltK"
                     /locus_tag="KJL94_001042"
     CDS             41022..41696
                     /gene="gltK"
                     /locus_tag="KJL94_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBD4409488.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41696..42421
                     /gene="gltL"
                     /locus_tag="KJL94_001043"
     CDS             41696..42421
                     /gene="gltL"
                     /locus_tag="KJL94_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBD4409489.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42539..43474
                     /gene="rihA"
                     /locus_tag="KJL94_001044"
     CDS             42539..43474
                     /gene="rihA"
                     /locus_tag="KJL94_001044"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBD4409490.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43558..45219
                     /gene="hscC"
                     /locus_tag="KJL94_001045"
                     /pseudo
     CDS             43558..45219
                     /gene="hscC"
                     /locus_tag="KJL94_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45279..46730)
                     /gene="djlC"
                     /locus_tag="KJL94_001046"
     CDS             complement(45279..46730)
                     /gene="djlC"
                     /locus_tag="KJL94_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBD4409491.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46727..47434)
                     /locus_tag="KJL94_001047"
     CDS             complement(46727..47434)
                     /locus_tag="KJL94_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD4409492.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47536..48090
                     /locus_tag="KJL94_001048"
     CDS             47536..48090
                     /locus_tag="KJL94_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD4409493.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48100..49527)
                     /locus_tag="KJL94_001049"
     CDS             complement(48100..49527)
                     /locus_tag="KJL94_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBD4409494.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49524..50231)
                     /locus_tag="KJL94_001050"
     CDS             complement(49524..50231)
                     /locus_tag="KJL94_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD4409495.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT        12185 a        12770 c        12899 g        12451 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactcagg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctacccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtg ctctggaaga agggatcaac
    11461 ctgctgccag gttttgcacc ggagctgcag ggtgtctata tcgaagaaca tgatggtcgt
    11521 aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg
    11581 gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga
    11641 cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg
    11701 ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg
    11761 cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg tatgggcggt tatgccgagt
    11821 atgctttcgg taaatccggt aactttattg cgaactatac ctacggcgtc tcactgctga
    11881 ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga
    11941 gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt gctgtggatt tgtaccgtgg
    12001 ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattaccgtg tgggggggca
    12061 ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg
    12121 ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta
    12181 tgacgctgtg ggcttttctt ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa
    12241 acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt
    12301 atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa
    12361 cggcaccatt tggcctggcc ttcgcacaga tgtttacacc agaagtgggt aaagtcatta
    12421 tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc
    12481 aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca
    12541 aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc
    12601 tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg
    12661 tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg
    12721 ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt
    12781 atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtga
    12841 ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac
    12901 acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgctcttttt ttattaaatg
    12961 tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt
    13021 gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga
    13081 cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccaa tagaagcacc
    13141 gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca cctgccaggg tgctggcgct
    13201 caccatttcc ggagacagct tagacagcgc cgctttttgt gcggaagtcg cagctgcctg
    13261 caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg
    13321 gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga tgccgtcttt
    13381 gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc
    13441 gccaaagccg aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccggtac
    13501 ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag aagaaaccag
    13561 cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat
    13621 cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg
    13681 gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcagacc
    13741 cgccatcgca cactcggagg agcagtccat acggatcgca ccgtctttat cgaggtgcat
    13801 aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc
    13861 gccaatgcgc ttccagtatt cgataccgga accgcccaac ggatcaacgc ccagcgtcag
    13921 gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca cgaacggctg
    13981 caccagatcc tgctctttca catagccgga tgccatcgct tcgtcgaggg agatacgctt
    14041 cacgcctttc aggccatcgg ccatcagtgc gttggccctg tcttccacca ctttggtgac
    14101 gttagtatca gccgggccac catttggcgg attgtacttg ataccaccat cttccggcgg
    14161 gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac
    14221 caggatggca ttggaaacgg caggcgtcgg agtgaagcca ttgttttcct gcacaatgac
    14281 atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc
    14341 gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc
    14401 aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc
    14461 acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag
    14521 tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg
    14581 gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc
    14641 cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca
    14701 tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat
    14761 gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaca
    14821 cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat
    14881 atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt
    14941 ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg
    15001 cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg
    15061 atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct
    15121 tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa
    15181 tccagtgcag tgtcttagct gaatacaagt cgccaagagt atatgaataa atcccggcag
    15241 cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc
    15301 tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca
    15361 tgaaattaaa tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc
    15421 ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa
    15481 acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagagccgg
    15541 taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca ctgcagatcg
    15601 acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac
    15661 tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg
    15721 tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa
    15781 ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt
    15841 ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc
    15901 agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta
    15961 tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat
    16021 ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac
    16081 cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa
    16141 cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat
    16201 tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag
    16261 atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga
    16321 ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc
    16381 aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg
    16441 gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgta atatgagccg tagcgaactg
    16501 atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt
    16561 tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct
    16621 gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca
    16681 agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac
    16741 cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga
    16801 cattgcaaaa agcagcaaag aagatctgga agcttacgac attctgctgc tgggcatccc
    16861 aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga
    16921 gattgatttc aatggtaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc
    16981 cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac
    17041 catcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggcaga
    17101 tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga
    17161 acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg aaattcttaa
    17221 tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca
    17281 aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccagtgttca
    17341 agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg
    17401 gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca
    17461 aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac
    17521 tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc gtttaaaaat
    17581 cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg
    17641 tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt
    17701 tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact
    17761 gacacagcaa catcaccacg atcacctgat ttgcctcgac tgcggcaagg ttatcgaatt
    17821 tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct
    17881 gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga
    17941 gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg cgggttggct
    18001 tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt tgttattact
    18061 gcgtatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat ttccggtttg
    18121 cgttgcacgc agacactgct gataatccag tacgcgtaca ctttcctggt cggcaaactg
    18181 ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt caattttttc
    18241 tggcgaacct tgcgcggtgt tgatacaggc gctgtatgcc tctttcagac ggctgtcttc
    18301 ctgtggtgcg gttgattgcg cacaagctac cagccccgat gccatcatgg cgatgagaat
    18361 cagttttttc attgaatgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
    18421 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc
    18481 gccgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
    18541 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc
    18601 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt
    18661 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
    18721 caggtcatac atcgcactga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
    18781 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttgggg tcatacgttg
    18841 caggaagtat ccctgctgac tgtcagcctt aatccaggtg ccttcgaggc gcaaatccac
    18901 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    18961 atcgttgacg ctgcgatcgt caactttatc gcgggtacca tagaactggt agctggtggt
    19021 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    19081 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    19141 accgatggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    19201 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaaggcacc
    19261 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ccggcataaa
    19321 gctccagtgc ggcgctaaca gcgtttgacc ggttggctga atgtaacctg ccctcgccca
    19381 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    19441 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    19501 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    19561 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    19621 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    19681 gataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    19741 cataaagccc gacattgctg taacaccggc aatagccagc gccagcgtac tacgtttgcc
    19801 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    19861 agcgaaaaaa aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg actacaggaa
    19921 cagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat gtaagaacat
    19981 gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgcg gttgaaaggc
    20041 ttgcagaaga ataaaaacgt gaaattccag caagatgaaa gcggttacat tttatttaat
    20101 tatgttttgt gattttaaat ttattttatg gataaattgc gaagcggaat taattcgcga
    20161 agaaaagcag aaaaaacgcc gcagagcgcg gcgtttggga ttgtttgcta tttcttgccg
    20221 gatgcggcgt gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag
    20281 atcatgtagg cctgataagc gaagcgcatc aggcaattta gcgtttgaaa tttattgtct
    20341 atttattgcc ggatgcgacg taaatgcctc acccggcttg gtagaactta aaattactcg
    20401 cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg tttttcggcc
    20461 gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct caaactggaa tgctttaccc
    20521 gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag cgattccggg
    20581 ttaatcaccg acaggaaatc atccgcagca cctgggttag gcacgctgaa cagacgatca
    20641 tacaaacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat aacacctttg
    20701 actttacgac catctgccgg atctttgctt aaggtgtcgg cgtcataagt acagaagatg
    20761 gtggtgatat taccttcggc atctttctcc acgcgttcag ccttaatgac gtaagcatta
    20821 cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg
    20881 aaatcggcgc gatcgatcca aatttcaccg ctaaacggca cctgacggct ccccatttcc
    20941 ggtttgttcg gatggttcgg catggtgacc atttcgcctt cgccctgata gttttcgata
    21001 accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt gagatcttca
    21061 cggatgcagg attccagcga cgccatctca atggtgttgt cctgcttggt cacgccgatg
    21121 cgtttgcaga actcacgaat agaagccgca gtgtaaccac gacgacgcag accggaaatg
    21181 gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag caggttcagc
    21241 ttacgcttgg acatcacagt gtattccaga ttcaggcgcg agaattcgta ctggcgcgga
    21301 tgaacaggaa tggtgatgtt atccagtacc cagtcgtaca gacgacggtt gtcctggaac
    21361 tcaagcgtac acagagagtg cgtaatacct tccagcgcat cgctgatgca atgggtgaag
    21421 tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc aaatttaatg
    21481 cggtacagca ccggatcgcg cattacgata aatggcgacg ccatgtcgat tttcgcacgc
    21541 aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag cgccaagttc
    21601 tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gctgcgtcag ggtaccgcgg
    21661 tattcgcgga tctgttccgg tgtcagttca tcaacgtacg ccaggccttt attgatcagt
    21721 tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac gttaccagac
    21781 cagtgaaaac ctaaccactc tacgtcgttt ttgatcgaat cgacatactc gatatcttct
    21841 tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc
    21901 ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt cggctccggc
    21961 gggaaacggg tgtgtactgt gatgtgctta ccactggcca gatcttcatc gatgatctga
    22021 cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa
    22081 caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt agttttttaa
    22141 aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg gcagagaaga
    22201 tctccaccgc ttgtcattgt tggatgcgac gctgccgtgt cgcatcaggc ataaagcaga
    22261 ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct gagctttaat
    22321 gagcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca tcgagcgggc
    22381 gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc ttacctgcgc
    22441 accctcttcc accagacgtt taaagccttt accttccagc gctacggtgt cgatacccat
    22501 atggacgacg atctccgcgc ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa
    22561 gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg gtttcaccgc
    22621 cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac
    22681 gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg gtacagcctg
    22741 cggttttgct acaggcgcgg cagttgccgg agttgcttca gctgacgcag cggctaccgg
    22801 accacgggca acgactttct tcatcgcatc gccgatggat tctgctttcg cgccaacaat
    22861 cacctgaata gtctgtttgt tcagtttcac tacaccagaa gcacccagac gtttacacat
    22921 cgtatcgttg acgcgggcag agtcagccac ggtcaggcgc agacgggtaa tacaggcatc
    22981 aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg ccagttgagt
    23041 cagaccttct tcagtattgc tgttggcttc ttcagtaacg atctcgtctt ctttatcttc
    23101 acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca cgaagtagat
    23161 agcgaagaag ataacgccca tcaccagcag catccagacg ttctggctgg cggcaggcag
    23221 gttatacatc aacgcgtagt cgatagcacc cgcagagaaa gagaagccgg catggatacc
    23281 cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag
    23341 cggcgcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca ggaacgcagt
    23401 aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg gtgctgcgaa
    23461 gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga agccggacat
    23521 gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt ggaaaaccgt
    23581 acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca gcacctgatg
    23641 cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac ccagcgcgcc
    23701 cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtaccggcg gccagacgta
    23761 accaaaaatg gccgccagca ccagacagaa gaagccggtg gcgatcggca caaagcgttt
    23821 gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt tataggctgc
    23881 gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt ctgggttgat
    23941 ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg ccgccgcacc
    24001 tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg cgaggttatc
    24061 aaaaattgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa
    24121 tcgcagcaac agcgccgcca ccggcagcac cgcgataggg agctgtaacg ccctaccgag
    24181 tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct atttggctcg
    24241 tttaaagccg tatttttatt ttgctgcaaa ttttactgcc gatgttctgt aatcagattg
    24301 ttagatcatc tgctacagag tgtgtgaaaa tttaattcgc atcgcaaatt aaacgcgtgt
    24361 cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg gctaaaccag
    24421 aaaacttatt ttatcattca aaaaatcaag tcggattgac gcctgtctgc gcaaatccag
    24481 gttacgctta aagatgtcta atccgccaac ggcttacatt ttacttattg aggtgaataa
    24541 tgagactgat ccccctgact accgctgaac aggtcggcaa atgggctgct cgccatatcg
    24601 tcaatcgtat caatgcgttc aaaccaactg ccgatcgtct gtttgtactg ggcctgccga
    24661 ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa gcaggccagg
    24721 tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg ccgaaagagc
    24781 atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt gatattccag
    24841 cagaaaacat caaccttctc aacggcaatg ccccggatat cgatgccgag tgccgccagt
    24901 atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg
    24961 acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa
    25021 ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg
    25081 tgccaaaata tgccctgact gtcggtgttg gtacactgct ggatgccgaa gaagtgatga
    25141 ttatggtgct gggtagccag aaagcactgg cgctgcaggc cgccgttgaa ggttgcgtga
    25201 accatatgtg gaccatcagc tgtctgcaac tgcatccgaa agcgatcatg gtgtgcgatg
    25261 aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa ttagaagcag
    25321 aaaatatcaa aggtctgtaa ttgttatccc tgccctggtt ccttgctcag ggcaatattt
    25381 tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc cggatcttta ccggccacga
    25441 atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg tctgtccggt
    25501 agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc tctcccccgg
    25561 ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca ccgctgaagc
    25621 ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag gctgtactaa
    25681 ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg tgcgcgttat
    25741 gcgtgagtac ctggcaaaac atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg
    25801 gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc
    25861 gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc tggcaccgga
    25921 aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg
    25981 tcactccaac gcgacgttga aagaagcaaa agccggtttc cgcgcgggga ttacctttgc
    26041 cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc
    26101 gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc atgttgatta
    26161 cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc
    26221 caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa caatatacta
    26281 ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct taaccatgat
    26341 tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag tgctgcgtat
    26401 ggcgacgctc tatccggcgc gtgcgattga cgttgaaaaa cgtctcggca cgctcgccgc
    26461 aggtaaagta gccaacctga ccgcattcac acctgatttt aaaatcacca agaccatcgt
    26521 taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg acaagctcag
    26581 ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg cctgattgac
    26641 cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc ccccgccagc
    26701 gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag
    26761 gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa tttccacgca
    26821 atcggcgtac ggcttggtcg tcatgacgcc accatcactc tgtttgatct cagcagcaaa
    26881 gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcagacgct ggaacatgcc
    26941 ctgctgaatg ccattgctca gtttattgat agctaccagc gcaagctgcg cgagctaatc
    27001 gccatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat tcattacatg
    27061 ccgcatattc aggtagaaaa ctgggggctg gtagaagcac tggaagaacg ttttaaagtg
    27121 acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta cttcggtgca
    27181 agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg ggccgggatt
    27241 atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat tggccatatt
    27301 caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct ggaaactatc
    27361 gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg ctaccagagc
    27421 cgtgtgccac tggacgactg caccatcaaa actatctgca aagccgcgaa caaaggcgat
    27481 agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc tgggtaaaac catcgccatt
    27541 gctatcaact tatttaatcc gcaaaaaatt gttattgctg gtgaaatcac cgaagccgat
    27601 aaagtgctgc tccctgctat tgaaagctgc attaataccc aagcgctgaa ggcgtttcgt
    27661 actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg
    27721 ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga aaattaatgt
    27781 gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc attaaaaatg
    27841 taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg
    27901 aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc aactatcctt
    27961 cgcagactgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat gtgccggata
    28021 gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga
    28081 aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc ggtttcacta
    28141 ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac acgttcctac aactgggaca
    28201 tgatgcataa agcagcctat ttcgtcgcta acggtgcacg ctttatcgcc accaacccgg
    28261 acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg attgagaaaa
    28321 tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc cgcgcggcat
    28381 taaacaaaat gcaggcgcat tcggaagaaa cagtgattgt cggcgataac ctgcgtaccg
    28441 atattctggc gggcttccag gccggtctgg agacgattct ggtgctttct ggtgtttcat
    28501 cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg tcagtcgctg
    28561 aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct gatgacaaac
    28621 gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa tatattgaat
    28681 tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaatcgca
    28741 ctttatcagc aatttggccc ccgtaaaata taaccattca ataaaacccg ctaaaaatta
    28801 tcgattcatc aataaataca tcctcacacc agaccctttt tcaccaatcg acaatcacca
    28861 ttacgttttt tatttttgca ctgccaaata gcttgcgcac agtgctgctt tgcggcattt
    28921 ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt aacggagaag gttatgtgtt
    28981 caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag aaagcactcg
    29041 agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata
    29101 acgccattct cgctcacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc
    29161 tctacaacca acaaaaaaac cacgtactgg cggtaaacgg tgaaatctac aaccaccagg
    29221 cattgcgcgc tgaatatggc gatcgttatc agttccagac agggtctgac tgtgaagtga
    29281 tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg
    29341 cctttgccct gtacgacagc gaaaaagatg cctacctgat tggtcgcgac catctgggga
    29401 tcatcccact gtatatgggg tatgacgaac acggtcagct gtatgtggcc tcagaaatga
    29461 aatcgctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc tatttgtgga
    29521 gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgacttt gatgcagtga
    29581 aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc gttaaaagcc
    29641 atctgatgtc tgatgtgcct tacggtgtgc tactttctgg tggtctggat tcctcaatta
    29701 tttccgctat caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa cgctctgaag
    29761 cctggtggcc gcagttacac tcctttgctg taggtctgcc gggttcaccg gatcttaaag
    29821 cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac
    29881 aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca
    29941 ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta
    30001 aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt tacttccata
    30061 aagcgcccaa cgctaaagaa ctgcatgaag agacggtgcg taaactgctg gccttgcata
    30121 tgtatgactg cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc
    30181 cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccgcag gataaaatgt
    30241 gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg
    30301 caagcgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg
    30361 acaccctgaa agaagtggcg gcgcagcagg tttctgatca gcaactggaa actgcccgct
    30421 tccgcttccc gtacaacacg ccgacctcaa aagaagcgta tctgtaccgc gagatctttg
    30481 aagaactgtt cccacttccg agtgccgctg agtgcgtgcc gggcggtcct tccgtcgcgt
    30541 gttcttcagc taaagcgatc gaatgggatg aagcgttcaa aaaaatggac gatccgtctg
    30601 gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga aatgcggtac tgccccggag
    30661 aaatccgggg ctgttttagt tattgcgctg cttgttgatt ctgatatgca gtaaggaaat
    30721 gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt atttcataga
    30781 ttaatttccc accactattg ttgaattccg aatttcatcc tgtacgacaa aagaatggct
    30841 gtagggtggg gaaaaacaca aagtaaacaa taattgacga atatagcgcc acgctgttcg
    30901 caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt gacgctgcaa
    30961 ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa aaagatggct
    31021 acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc
    31081 gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt
    31141 tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg
    31201 ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg
    31261 gttcgaatcc aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg
    31321 gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca
    31381 ggtaccccag ccatcgaaga aataatctgg ctacgtagct cagttggtta gagcacatca
    31441 ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc
    31501 gaatacgttc ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc
    31561 ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga
    31621 cgcttacatc gtaagtgcac ccagttgggg tatcgccaag cggtaaggca ccggattctg
    31681 attccggcat tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg
    31741 gcgagctttt tgcttttctg cgttcattca atgtcgaatg cgatgttgac acgtcttatc
    31801 cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc tacggctggc acgcatccgg
    31861 caatgttgta aggctacaac cctaacgcat atttcagcgc ctgacgtttc aacacgccag
    31921 cacgctctgc cgccattaac ccgagattac gcacaaaacg cagcggcggc agattattac
    31981 tgaatccggc ataaaacaga tccataccac tttgcataat gaagttatcc gccatgcgcc
    32041 gcatctggta acgcttgagg acaggataac tggcccacgc ttcaccgtag ctgcgggcat
    32101 tcaccagaac atcaatcagg gcatcgacat cacgataacc aagattcacc ccctgccccg
    32161 ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc aagccccggc tgcacatact
    32221 gcaacgcatg tcggcgcgtc agcggaaacg caccagcggc aagcggtgta acgtaaccca
    32281 gacgcgacgg gaaatgcttc gcgatttccg cctggagttg cgccatattc atattctgca
    32341 actggcgaat acgcgccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg
    32401 gcagaaacgc acgcggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt
    32461 tctcacactg tacgctaatc aacatgcacg actgcgcata ctgccaggca tgaacgccaa
    32521 ttcccgccat ctgccgcacc tgagaatttg cgccgtcggc accaatcacc agtttcgcgc
    32581 ggatcgtttc accgcccttc agttccagct cctgaagatc attatctcga tgtaatgcaa
    32641 tcagcgaggt tggcacacgt aacgttactt tcggatgcgc ttccagcgcc tgccacaacg
    32701 cctgttgcag gactgtgttt tccaccatgt agccaagcaa cggcaacttt aattcggctg
    32761 cgtcaaatac cacatgtgcc gtttcccact cccaggtttc cagtctgcgg taaggatggc
    32821 aacgcatagc ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgaagccg
    32881 cgctgatcgc cgagatccgc acgtccggtt ggctgtcagc gacaaacggt gcaggttctg
    32941 cgtgctcgat caccgttacc gaaaatccgt gctgtgccag ccccagcgcc agtgcgccgc
    33001 cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc
    33061 ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat
    33121 actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg ctactatttc
    33181 gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg
    33241 agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg
    33301 acgtggcgga agaagcggat gtgctactgc tgaacacctg ctcaatccgc gagaaggctc
    33361 aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc
    33421 tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg
    33481 cccactatgt cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca
    33541 actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt
    33601 ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag
    33661 gctgcaataa atattgcacc tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc
    33721 gtccgtctga cgatattctg tttgagattg cccagcttgc ggctcagggc gtgcgtgaag
    33781 tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga gaactacgac ggcaccaccg
    33841 gatcgtttgc cgatctgttg cgtctggttg ctgcgatcga cgggatcgat cgtattcgct
    33901 ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtac cgcgacacgc
    33961 ctgagctggt gagcttcctg catctgccgg tacagagcgg ttccgatcgc attctgaacc
    34021 tgatgggacg tacccatacg gcgctggagt acaaagcgat catccgtaaa ctgcgtgcgg
    34081 cgcgtccgga tattcagatc agttctgact tcattgttgg cttccctggc gaaaccaccg
    34141 aagacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct
    34201 ttatcttctc tgcacgtcca ggaacgcctg ccgccgatat ggttgatgat gttccggaag
    34261 aagagaagaa gcagcgtctg tatattctgc aagaacgtat caatcagcaa gcgatggcat
    34321 ggagccgtcg gatgctcggc accacccagc gtattctggt agaagggact tcgcgtaaga
    34381 gcatcatgga gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc gagggcacac
    34441 cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc
    34501 gcggtaaagt ggtgcgaact gaagatgaaa tgggtctgcg catggcagaa acaccggagt
    34561 cggtgattgc ccgcacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat
    34621 tctcaggcct gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc
    34681 aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag
    34741 cttccggcgg catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct
    34801 ggagccagca gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa
    34861 gcagctcgaa cgccgtctcg gcatcgagat caatcgccgc gataaccact ttaaactgac
    34921 cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac
    34981 tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa
    35041 agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg gagtacggca aagcggtcaa
    35101 tatcaaaact aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc
    35161 caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta
    35221 cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc gtattctgct
    35281 gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca
    35341 gaaagtagac ccgtatctgc gcccactgta cgacgcgctg tttgaaatgc tgggctttga
    35401 gaaagtcgag aaactgattg agcgcaacgt tattgaagtc gcgccgctgg cctatatgcg
    35461 tggtcgtacg ctgaacgacg cgtttatcat tctcgatgag agccagaaca ctaccatcga
    35521 acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga
    35581 cgtcacacag atcgacctgc cgcgtaatac taaatcaggc ttacgtcatg ccattgaagt
    35641 gctggccgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca
    35701 cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg gaagaagcag aacaaaaacg
    35761 taaagcggcg ctggcagcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg
    35821 atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt
    35881 caaacatggc tgaatgcggt gatcccgcag tttcaggaag agtcggaagt gactattcgc
    35941 gtggtcgata ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg
    36001 accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggagatgtc gctactgggc
    36061 gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg
    36121 gaggcgcatt gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac
    36181 attgaagatg acgaagcaga agaaatggaa gccctcgaaa cagagattat gcttgctctg
    36241 ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga
    36301 cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag aacccatacg
    36361 acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt
    36421 tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg
    36481 cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg
    36541 aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga
    36601 tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg
    36661 cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg
    36721 cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt
    36781 tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc
    36841 gctctcagcg ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt tccggtctgg
    36901 tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag
    36961 aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc gcactggctt
    37021 ccattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagaa gtcgacacta
    37081 tcggtggtct ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa actatcgaca
    37141 tcgacggtta ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt caggttcatg
    37201 tcaaaatccc ggatgactca ccccagccaa agctggatga ataaaaccga aactggatag
    37261 ataactacat ggcttttgcc tcattaattg aacgccagcg tattcgcctg ctgctggcgt
    37321 tattattcgg tgcctgcgga acgctggcct tctctcctta cgacgtctgg cctgcggcga
    37381 ttatttcgct gatggggctt caggcgttga cctttaaccg tcgcccactc cagtctgccg
    37441 ctattggctt ttgctgggga tttggcctct ttggcagcgg tattaactgg gtctatgtca
    37501 gcatcgcgac ctttggcggg atgcctggcc cggttaacat cttcctggtg gtgctgctgg
    37561 cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt ctgtggccga
    37621 aaaccacctg gctgcgcgtg gcgattgcca cccctgctct ctggcaagtg accgagtttc
    37681 tgcgcggttg ggtgctgact ggcttcccgt ggttacagtt cggctatagc cagattgatg
    37741 gcccgttaaa agggctggca ccgctaatgg gcgtggaagc cattaacttc ctgctgatga
    37801 tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg ctggtggtgg
    37861 ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat ccaatggttt accccgcaac
    37921 cggagaaaac cattcaggtt tcgatggttc agggcgatat tccgcaatcg ctgaaatggg
    37981 acgaaggcca gcttcttaat acgctaaaga tttactacaa cgcaacggca ccgctgatgg
    38041 gcaaatcatc gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc
    38101 aaccgttcct caaagcactg gacggtgagt tgcgtgataa aggtagctcg ctggtaaccg
    38161 ggattgtcga cgcgcgtctc aataagcaga accgctacga tacctacaac accatcatca
    38221 cgctgggtaa aggtgcaccg tacagctacg aatcagccga tcgctataac aaaaaccatc
    38281 tggtgccgtt tggcgagttt gtcccgctgg agtcgattct gcgtccgtta gcaccgttct
    38341 ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg ctgtcggcaa
    38401 atggtattga gcttactgcg gctatttgct acgagatcat tctcgccgag caagtgcgcg
    38461 ataacttccg cccggatacc gactacctgc tgactatctc caacgatgcg tggtttggta
    38521 aatctattgg tccatggcaa cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc
    38581 gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggtgaga
    38641 ttcaggcgat gatcccgcag ttcgcccgcg aggtgttaac cactaacgtg acgccgacca
    38701 ccggactcac accatacgca cgtaccggca actggccgct gtgggtgctg acggcactgt
    38761 ttggttttgc tgctgtgttg atgagtctgc gtcagcgacg taaataatcc cttcttatcg
    38821 tgccggaata catcccatat ttcggcacgc tcaccggatg cggcatcagt gcattatccg
    38881 gataacaata ttctcctatc caactttggc acatctattg ctttgttata caaggcaaag
    38941 cttgaaccag catcagtaca acccggttgc accacagcaa tccaccggta gcaccaaaag
    39001 agtgcatttt aagaattttg cttcagaatg gtgcggtgca cctcgcaaaa acaaacaata
    39061 aaacatcaat atctttacat ttagccaaac taaatgttac ctaacaggca caacactgca
    39121 caataaagtt gcagacgata acaacacaaa cactcacaac gggtatccat gcgttcttaa
    39181 cgcagaagat aaaggtgttg gatatgcaat tacgtaaacc tgccacagca atcctcgccc
    39241 tggcgctttc cgcaggactg gcacaggcag atgacgccgc cccggcagca ggcagtacgc
    39301 tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct tcagtacctt
    39361 tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac tccaacgcca
    39421 ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa ctgattccga
    39481 ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt gaatgtggtt
    39541 ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact attttcgtgg
    39601 tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc gacctgaaag
    39661 gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac aaactgaatg
    39721 aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac tctttccgca
    39781 ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg gccggtgaac
    39841 gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag tctcaggaag
    39901 cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg gatgacacta
    39961 tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg ttcaaaaatc
    40021 caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg aaagcactgt
    40081 tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg gaaattccag
    40141 ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt ccccatcggg
    40201 cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac gctgctaccc
    40261 tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt tttacaacaa
    40321 gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca ggtgacgatc
    40381 gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt cggcatttta
    40441 cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga actgttccgc
    40501 aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga gctgctgccg
    40561 gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt tttcctttca
    40621 tccatgctct gcctggggct gtttaccgcc gcccgtgttt gcgaacaggt acgcgccgct
    40681 attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct gacgctaccg
    40741 cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc gccgatgact
    40801 tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg tctggtggat
    40861 atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga gtcgtttacc
    40921 gccatcacgc ttgcgtatgt gttgattaac gcttttatca tgctggtgat gacgctggtt
    40981 gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag tttgactgga
    41041 gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc ctgaaaatca
    41101 ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg atgcgcttgt
    41161 ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc cgctcgattc
    41221 ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg caaaatgtgc
    41281 tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg ttttcgatgt
    41341 ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt tcccgtggtc
    41401 agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa ctgattattc
    41461 tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc gtactgttcc
    41521 aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc gcctcaacca
    41581 ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt gtttatttcg
    41641 ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca gcataatgat
    41701 taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg actgctcaac
    41761 cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg gcaaatcaac
    41821 gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca ccgtcgatgg
    41881 tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg tcgggatggt
    41941 attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga ccctggcgca
    42001 ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga aattgctgga
    42061 gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg gcggtcagca
    42121 gcagcgcgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc tgttcgacga
    42181 accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga tggtggaact
    42241 ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg cccgcaaagt
    42301 ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc cgaaagacgc
    42361 tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa tcctgcatta
    42421 atcactctgg cgcacatacc caattgcgcg ccatctcacg ctttatcgtt tcgcccctcg
    42481 tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag gagcaacaat
    42541 ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg caatagttct
    42601 cgcactcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg gaaaccagac
    42661 accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc gcaccgatat
    42721 tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg cggacaatgt
    42781 gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg caccgcaaaa
    42841 ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac ctgtcacgat
    42901 tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc cggaactgca
    42961 tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta actggacgcc
    43021 tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct tccagtcagg
    43081 gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc acgttgaaga
    43141 caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg aactgctgga
    43201 tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac cgctgcatga
    43261 cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg agcgctgggt
    43321 tggcgtggaa acacagggga aatataccca gggtatgacg gttgttgatt attattatct
    43381 gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg gctttgttga
    43441 tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta agatcaccac
    43501 gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat aaatgcaatg
    43561 gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt aattgccgtc
    43621 tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt aacaccatcc
    43681 ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt atcacggcgt
    43741 acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag ctggcggtta
    43801 ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa
    43861 gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctccgt tccggcttat
    43921 ttcagtgatg aacaacgcaa gcatacccgt ttagcagcgg acttagccgg gttaaatgcg
    43981 gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca tacccaacaa
    44041 aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag
    44101 tacgccacgc cggtgattga agttcacgcc tccgctggcg acaacttcct cggtggcgaa
    44161 gattttaccc atatgttggt cgatgacgtt ttaaagcgcg cggatgtcac aaggaccacg
    44221 cttaacgaga gtgaactggc agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat
    44281 caatcgccaa tgcacattcg ctggtagtac caggatgaaa cgcgggaatg cgaattttac
    44341 gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa
    44401 caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt gctggttggc
    44461 ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg
    44521 tatcaaagtt acgatccgag caccattgtc gcgctgggcg cagcaatcca ggccgcctgc
    44581 cgcttacgca gtgaagatat tgaagaagtg atcctcactg atatttgccc ttactcgttg
    44641 ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct cgccgattat tgaacgaaac
    44701 accactgtgc ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga acaggattca
    44761 attacggtta acgtctatca gggagaaaac cacaaagtta aaaacaacat tctggtggaa
    44821 tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt cgattgatat tcgctttagt
    44881 tatgatatca acgggttgct tgaagttgac gtgcttctgg aagacggcag cgttaagtcc
    44941 agagtgatta accacagccc ggtaacattg agcgcgcagc agattgaaga gagtcggacg
    45001 cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc
    45061 aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggccgggtg
    45121 atcaccgatt ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg
    45181 cggcgggcga gcgtttattt agccattgag acctcataat cacggctgcc ggatgcggcg
    45241 taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt
    45301 ttgccgagag cataacctat aaccagcacc gttaaactga ttttgataat gctggtaatg
    45361 ctctgtggtt tttcaccctc atcatttttt tcttcatggt tttcaggtgt tggctcatcg
    45421 acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt gataatggcc
    45481 tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag catctcttgt
    45541 ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa
    45601 tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag
    45661 aataacgact caataaacga ttgacctaac ggcgataatt ttccgctatg taaaatttcc
    45721 gcccataaca gccgcgtccg ggcgctctca ccaggaacat atttcagcgg ttgaccttgc
    45781 gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa
    45841 tgacgcttgc tccactgcaa caacaaatta tcagcttgtg tagatgggta actttcccag
    45901 tacgcgccga gatcagcaag caggctttcc ccctcgccgc agtacaaacg ttgtgcacac
    45961 tggtaatacc gggcatcttc gctttctggt tcttccgctt gccactgttt ggctacagga
    46021 atgagttcca ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac
    46081 tttgcatcat caggaatgaa ccacggtcct tccatcgcca aaaacgcagt gacatagtca
    46141 ggatggtaac ggcagcaacg ttccagcgcg aagtaatatt caacagtctg gttctgtaac
    46201 gcgacgggat attccagaag gctaaggaaa tcgaaacaat caccagcctg aatggcctca
    46261 agaaaagcct ccctttcttc ctcatccatt ccttcgctgt cattaaacga atgccagttc
    46321 aaacgctctg ccaacaaaga agcacaattt aatgacagat atcgcgcttc tatagcgatt
    46381 gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga
    46441 ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag taatgtcctg
    46501 aatgcacgta gtatttcatg ttcagcggtg gcatcttttt cttcatcatc tgcttcctcg
    46561 acaggattta ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc tttaaacccc
    46621 tgcggatcgg tttcgggatg acacaacggt aagcgagcaa gataagcctg gcggataatg
    46681 tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca
    46741 catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg
    46801 catagaaacg gtcgcttccc gctgccagct cttcgaacat ccgactcccg gcgctatcgt
    46861 atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc
    46921 agtagagacg ccctagtgaa taggcagaaa aatagtgcat ccagctgtga tagtaatggt
    46981 gagctcgtgc atagacgcgc gattgcagcc ataaactctc ttcttccgtc agccagttgt
    47041 tgagcacacc ggcccgtgac agaaacccca ttcgaacata atcccaggct ttgattcccc
    47101 cttctccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
    47161 agccaccagc aattaatgct ttccactctt caggggaaga gcgttgccat gtcagatata
    47221 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
    47281 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca
    47341 tttttggcga gtcatgcgcg ggtttgcaac tctgattaag tatttccatg ggcgcagaaa
    47401 gcgcaaaaag aagatattgt tcttccttat tcatttttta ataatccctt aagtgcatta
    47461 actgactact tttttattat tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa
    47521 tcgaagcatt cacacatgaa taaaaaatta atgtatatat tcgcaatttt tatagttgca
    47581 gcaattacct gtattagcca acccaagaaa acgatgttgc gtgataaagc catggtgaat
    47641 tatgcctttg attatttaag atcaccaggc agtctaccgt taaccacggc agccacggag
    47701 ctttccgcga ttcatggtca ctcaacatcg caatatcgcc ttggcgaatt ttatcttcat
    47761 ggtagcgacg gtaaaccact ggattataca caggcgagat actggtatga gcaatcagca
    47821 gaacaggaaa atccgcgcgc acaaagcaaa ctgaggtgga tctacctgaa aggcctggga
    47881 gtcaaacccg atacccgtaa agcaattctc tggtataagg aagcagctga acaagggtat
    47941 gctcatgctc aatatacttt gggtttgatc tacagaaatg gcactggtat taatgttaac
    48001 cattatgaat ctcaaaaatg gttaaaactg gccgccaaac aacattacaa aaatgcggaa
    48061 agattacttg ccggacttcc cgcacattaa cgtcgactgt cacccgaaga gatggagtat
    48121 tttccctatc aaaccggcaa agataaaaat gtagaaaata attttgatga cgcccagaca
    48181 gcccagcccg ccatcatcaa cgggcattgg ctgtggttgc ggctccggct cgacggtttc
    48241 ctgaccggta aaattcgccg cctgaattaa taatcgacga ataattaatg cttccagtgg
    48301 acaacgatct tctgcatcaa ggcggctcac aatcggcatt aatgattctt ctgtcggttg
    48361 gctggcctgc actaattgtt tggcaactaa atataatggc cataagggat gttctgtatc
    48421 acgcgtgagt gagatccgcg gcttacgtcc ttcgtgcaaa accatctcga ccagtgaacg
    48481 ccccagcgca gagagttttt catcgctgag tatttcgtat aaatgaaaac gcgtacgggc
    48541 gctgtcgccg gggacataaa gtagcggttt tccctgatcg ttaaccagat cacgcgcttc
    48601 aatcatcatc accactaatg ggtaataata gacccgatgc tgacggcacc attgcaacat
    48661 taaagcatcc gcctgggtgg agggatactc gcgccagtag taacaaagtt ctgccagcag
    48721 gctttcgcct tctccacaat agacgcgttg tgcgtattca taataacgcg catcttcatt
    48781 gtcaggttca gcttcccgcc acgaccgggc gtaatccagg agttcgggga ttcccaggcg
    48841 gcttgcgcta taccagcgga gtaaatttct gtgcagtttt gcatcatcgg ggataatcac
    48901 tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga tgcgatgacc aaatccgagc
    48961 gaacatttgg taaaaatcga tggtgtcatt ttgcacggca actggcagat gcagaatggt
    49021 ctggaagtta aaaacattcc ccttcgcaat ggcatataaa aaggattcca gttcttcttc
    49081 gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat ctttctgcta ataacaccac
    49141 gcactcgaag gataaatggg cagtgttcat ggcgattgtg cacagcgacc agcgtaattc
    49201 atcaatctct tccatcgagc aataatttaa ttgctgaatg aatcgctgcc aggcggagga
    49261 cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag gcgagcagaa tttcatgttc
    49321 tgctacctca tattcttctg gttgccaaac agatttagca ggcgactgcg caatccgtag
    49381 cgcctcctca tacgcctgac gaagttgttt aaaaccctgc ggatcggttt ctggatgaaa
    49441 ggacggtaac agcgccagat aagcgcggcg gataatatcg acgtcagtcg tttcctctat
    49501 atcgaggatc ttccagcaat ttttcataag tcgctcatat ccttaagcgt ttccgggcat
    49561 gtggggtagt cagccagtgg ttgccaggac aaggtagggt aatacgcatc atcactggca
    49621 aataattgat aaaacatatc attgccggaa tcgtcgtatt ctttacgcgc taagaattcg
    49681 cgtaataaag gcagatgatc atcttcggga gactgccaat attgccgacc aaaggtataa
    49741 ccggcaaagt attgccgcca gttactataa taacgtagag cccgtagatg aatacgcgac
    49801 tggatccata gactttcttc ttcactcaac cagttattaa gtacgcccat tcgacttaaa
    49861 aaacccatcc ggacataatc ccatgcttta attcctccct caccacagca ttcggctgtg
    49921 ctggcgacaa attgcgcata agcctgacct tgctcattta attcagcaag atagtcacgc
    49981 cattcacatg gagaataacg aaaccagcgg tgataaaacc ctgccaaccg agcggcatga
    50041 ccgttctcga tcattcggtg aattatgcgc agcaggtcat cacggtcatc aattccccat
    50101 gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt tgggggatga atgcgaaggt
    50161 aaacagcatt cattgcgtat ttccatcgga gtggaaagtg caaacaatat tttttgcgac
    50221 cccatgtcca tataatttat ccttaaacat tcatttaatg aatgtaaact acatttcgag
    50281 gctcagttat ttatactttt ctgac
//
LOCUS       DAEBXD010000018        49769 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEBXD010000018 DAEBXD010000000
VERSION     DAEBXD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49769
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.018"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..219
                     /locus_tag="KJL94_001051"
     CDS             <1..219
                     /locus_tag="KJL94_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4409496.1"
                     /translation="SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIE
                     YYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            321..473
                     /gene="hokA"
                     /locus_tag="KJL94_001052"
     CDS             321..473
                     /gene="hokA"
                     /locus_tag="KJL94_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBD4409497.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(660..872)
                     /gene="cspA"
                     /locus_tag="KJL94_001053"
     CDS             complement(660..872)
                     /gene="cspA"
                     /locus_tag="KJL94_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBD4409498.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1153..1443)
                     /locus_tag="KJL94_001054"
                     /pseudo
     CDS             complement(1153..1443)
                     /locus_tag="KJL94_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1877..2587
                     /locus_tag="KJL94_001055"
     CDS             1877..2587
                     /locus_tag="KJL94_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBD4409499.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="KJL94_001056"
     CDS             complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="KJL94_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBD4409500.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3715..4365)
                     /locus_tag="KJL94_001057"
     CDS             complement(3715..4365)
                     /locus_tag="KJL94_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBD4409501.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4528..6861
                     /gene="bisC"
                     /locus_tag="KJL94_001058"
     CDS             4528..6861
                     /gene="bisC"
                     /locus_tag="KJL94_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBD4409502.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6830..7270)
                     /locus_tag="KJL94_001059"
     CDS             complement(6830..7270)
                     /locus_tag="KJL94_001059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4409503.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7267..7830)
                     /gene="tag"
                     /locus_tag="KJL94_001060"
     CDS             complement(7267..7830)
                     /gene="tag"
                     /locus_tag="KJL94_001060"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBD4409504.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7988..8686
                     /locus_tag="KJL94_001061"
     CDS             7988..8686
                     /locus_tag="KJL94_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4409505.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8915..10123
                     /locus_tag="KJL94_001062"
     CDS             8915..10123
                     /locus_tag="KJL94_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4409506.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10447..12138
                     /gene="eptB"
                     /locus_tag="KJL94_001063"
     CDS             10447..12138
                     /gene="eptB"
                     /locus_tag="KJL94_001063"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBD4409507.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12230..12306
                     /locus_tag="KJL94_001064"
     tRNA            12230..12306
                     /locus_tag="KJL94_001064"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12264..12266,aa:Pro,seq:cgg)
     gene            13050..14657
                     /gene="dppA"
                     /locus_tag="KJL94_001065"
     CDS             13050..14657
                     /gene="dppA"
                     /locus_tag="KJL94_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBD4409508.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14965..15984
                     /gene="dppB"
                     /locus_tag="KJL94_001066"
     CDS             14965..15984
                     /gene="dppB"
                     /locus_tag="KJL94_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBD4409509.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15994..16896
                     /gene="dppC"
                     /locus_tag="KJL94_001067"
     CDS             15994..16896
                     /gene="dppC"
                     /locus_tag="KJL94_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBD4409510.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16907..17890
                     /gene="dppD"
                     /locus_tag="KJL94_001068"
     CDS             16907..17890
                     /gene="dppD"
                     /locus_tag="KJL94_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBD4409511.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17887..18891
                     /gene="dppF"
                     /locus_tag="KJL94_001069"
     CDS             17887..18891
                     /gene="dppF"
                     /locus_tag="KJL94_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBD4409512.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18921..20192)
                     /locus_tag="KJL94_001070"
     CDS             complement(18921..20192)
                     /locus_tag="KJL94_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD4409513.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20668..20775
                     /locus_tag="KJL94_001071"
     CDS             20668..20775
                     /locus_tag="KJL94_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4409514.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21151..21258
                     /gene="ldrD"
                     /locus_tag="KJL94_001072"
     CDS             21151..21258
                     /gene="ldrD"
                     /locus_tag="KJL94_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBD4409515.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21634..21741
                     /locus_tag="KJL94_001073"
     CDS             21634..21741
                     /locus_tag="KJL94_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4409516.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22117..22224
                     /locus_tag="KJL94_001074"
     CDS             22117..22224
                     /locus_tag="KJL94_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4409517.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22311..23990)
                     /gene="bcsG"
                     /locus_tag="KJL94_001075"
     CDS             complement(22311..23990)
                     /gene="bcsG"
                     /locus_tag="KJL94_001075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBD4409518.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23987..24178)
                     /gene="bcsF"
                     /locus_tag="KJL94_001076"
     CDS             complement(23987..24178)
                     /gene="bcsF"
                     /locus_tag="KJL94_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBD4409519.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24175..25746)
                     /gene="bcsE"
                     /locus_tag="KJL94_001077"
     CDS             complement(24175..25746)
                     /gene="bcsE"
                     /locus_tag="KJL94_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBD4409520.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26019..26207
                     /locus_tag="KJL94_001078"
     CDS             26019..26207
                     /locus_tag="KJL94_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBD4409521.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            26219..26971
                     /gene="bcsQ"
                     /locus_tag="KJL94_001079"
     CDS             26219..26971
                     /gene="bcsQ"
                     /locus_tag="KJL94_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBD4409522.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26968..27327
                     /locus_tag="KJL94_001080"
     CDS             26968..27327
                     /locus_tag="KJL94_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBD4409523.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27324..29585
                     /gene="bcsA"
                     /locus_tag="KJL94_001081"
                     /pseudo
     CDS             27324..29585
                     /gene="bcsA"
                     /locus_tag="KJL94_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29596..31935
                     /gene="bcsB"
                     /locus_tag="KJL94_001082"
     CDS             29596..31935
                     /gene="bcsB"
                     /locus_tag="KJL94_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBD4409524.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            31942..33048
                     /gene="bcsZ"
                     /locus_tag="KJL94_001083"
     CDS             31942..33048
                     /gene="bcsZ"
                     /locus_tag="KJL94_001083"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBD4409525.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            33107..33283
                     /locus_tag="KJL94_001084"
                     /pseudo
     CDS             33107..33283
                     /locus_tag="KJL94_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            33611..35359
                     /gene="bcsC"
                     /locus_tag="KJL94_001085"
                     /pseudo
     CDS             33611..35359
                     /gene="bcsC"
                     /locus_tag="KJL94_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            35441..37429
                     /gene="hmsP"
                     /locus_tag="KJL94_001086"
     CDS             35441..37429
                     /gene="hmsP"
                     /locus_tag="KJL94_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBD4409526.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37615..38901
                     /gene="dctA"
                     /locus_tag="KJL94_001087"
     CDS             37615..38901
                     /gene="dctA"
                     /locus_tag="KJL94_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBD4409527.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            39122..40618
                     /locus_tag="KJL94_001088"
     CDS             39122..40618
                     /locus_tag="KJL94_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD4409528.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(40714..41643)
                     /gene="kdgK"
                     /locus_tag="KJL94_001089"
     CDS             complement(40714..41643)
                     /gene="kdgK"
                     /locus_tag="KJL94_001089"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBD4409529.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41875..42642
                     /gene="pdeH"
                     /locus_tag="KJL94_001090"
     CDS             41875..42642
                     /gene="pdeH"
                     /locus_tag="KJL94_001090"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBD4409530.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            42712..44772
                     /locus_tag="KJL94_001091"
     CDS             42712..44772
                     /locus_tag="KJL94_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD4409531.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44954..46276)
                     /locus_tag="KJL94_001092"
     CDS             complement(44954..46276)
                     /locus_tag="KJL94_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBD4409532.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46687..47700)
                     /gene="yhjD"
                     /locus_tag="KJL94_001093"
     CDS             complement(46687..47700)
                     /gene="yhjD"
                     /locus_tag="KJL94_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBD4409533.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47749..48648)
                     /locus_tag="KJL94_001094"
     CDS             complement(47749..48648)
                     /locus_tag="KJL94_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4409534.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            49168..>49769
                     /locus_tag="KJL94_001095"
     CDS             49168..>49769
                     /locus_tag="KJL94_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD4409535.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11929 a        12410 c        13437 g        11993 t
ORIGIN      
        1 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
       61 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      121 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      181 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      241 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      301 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      361 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      421 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      481 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      541 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      601 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
      661 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
      721 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
      781 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
      841 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
      901 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
      961 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1021 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1081 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1141 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1201 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1261 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1321 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1381 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1441 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1501 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1561 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1621 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     1681 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     1741 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     1801 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     1861 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     1921 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     1981 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2041 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2101 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2161 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2221 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2281 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2341 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2401 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2461 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2521 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2581 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     2641 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     2701 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     2761 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     2821 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     2881 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     2941 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3001 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3061 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3121 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3181 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3241 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3301 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3361 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3421 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3481 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3541 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3601 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     3661 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     3721 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     3781 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     3841 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     3901 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     3961 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4021 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4081 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4141 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4201 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4261 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4321 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4381 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4441 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4501 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4561 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4621 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4681 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4741 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4801 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4861 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4921 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     4981 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5041 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5101 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5161 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5221 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5281 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5341 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5401 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5461 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5521 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5581 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     5641 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     5701 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     5761 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     5821 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     5881 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     5941 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6001 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6061 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6121 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6181 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6241 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6301 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6361 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6421 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6481 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6541 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6601 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     6661 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     6721 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     6781 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     6841 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     6901 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     6961 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7021 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7081 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7141 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7201 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7261 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7321 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7381 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7441 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7501 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7561 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7621 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     7681 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     7741 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     7801 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     7861 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     7921 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     7981 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8041 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8101 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8161 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8221 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8281 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8341 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8401 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8461 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8521 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8581 gttgctcaat caaaatattg ccgcctatgc tgcgctaact caggcagaaa ataccactaa
     8641 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     8701 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     8761 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8821 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     8881 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8941 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9001 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9061 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9121 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9181 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9241 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9301 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9361 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9421 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9481 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9541 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9601 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9661 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9721 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9781 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9841 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9901 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9961 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10021 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10081 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10141 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10201 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10261 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10321 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10381 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10441 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10501 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10561 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10621 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    10681 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    10741 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    10801 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    10861 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    10921 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    10981 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11041 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11101 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11161 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11221 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11281 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11341 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11401 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11461 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11521 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11581 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    11641 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    11701 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    11761 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    11821 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    11881 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    11941 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12001 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12061 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12121 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12181 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12241 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12301 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12361 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12421 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12481 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12541 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12601 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    12661 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    12721 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    12781 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    12841 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    12901 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    12961 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13021 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13081 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13141 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13201 ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt aaaatcggca
    13261 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13321 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13381 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13441 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13501 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13561 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13621 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    13681 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    13741 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    13801 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    13861 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    13921 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    13981 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    14041 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14101 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14161 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14221 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14281 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14341 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14401 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14461 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14521 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14581 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    14641 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    14701 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    14761 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    14821 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    14881 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    14941 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    15001 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15061 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15121 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15181 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15241 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15301 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15361 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15421 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15481 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15541 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15601 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    15661 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    15721 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    15781 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    15841 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    15901 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    15961 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    16021 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16081 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16141 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16201 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16261 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16321 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16381 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16441 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16501 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16561 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16621 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    16681 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    16741 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    16801 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    16861 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    16921 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    16981 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    17041 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17101 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17161 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17221 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17281 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17341 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17401 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17461 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17521 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17581 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    17641 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    17701 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    17761 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    17821 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    17881 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    17941 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    18001 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18061 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18121 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18181 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18241 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18301 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18361 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18421 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18481 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18541 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18601 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    18661 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    18721 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    18781 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    18841 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    18901 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    18961 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    19021 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19081 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19141 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19201 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19261 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19321 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19381 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19441 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19501 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19561 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19621 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    19681 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    19741 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    19801 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    19861 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    19921 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    19981 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    20041 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20101 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20161 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20221 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20281 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20341 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20401 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20461 aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
    20521 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    20581 tacctgacaa cataacgggg gctaatcttg aacctagacc actcaagaat agccgcgaaa
    20641 cgttgtcatt acaacacagg cggctatatg acgctcgcag agctgggcat ggccttctgg
    20701 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    20761 aacaagcgga agtagcgtgt catgtgggcg tcaggctgcc gtaatggcaa tttgcgcccg
    20821 gaccaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
    20881 agcggataat attctgcgga atgcgcagca cagagtgaag atgagacgga ttccggaaca
    20941 gaaaaacagt cgtagaatca gcgtcaagcc ggaaggattc cggtgaggcg caatgttgcg
    21001 ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    21061 atgcacctga caacaccacg ggggccattc ttgcatccaa acaatacaag aatagccgcg
    21121 aaacgttgtc attacaatac aggcggctat atgacgttcg cagaactggg catggccttc
    21181 tggcatgatt tagcggctcc ggtcattgct ggtattcttg ccagtatgat cgtgaactgg
    21241 ctgaacaagc ggaagtaacg tgccatgcgg gcgtcaggct gccgtaatgg caatttgcgc
    21301 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    21361 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagacgtgac ggattccgga
    21421 acagaaaaac agtcgtagaa tcagcgtcaa gccggaaggg ttccggtgag gcgcaatgtt
    21481 gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    21541 ggtatgcacc tgacaacatc acgggggcca ttcttgaacc tgaacaactc aagaatagcc
    21601 gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc tcgcagaact gggcatggcc
    21661 ttctggcatg atttagcggc tccggtcatt gctggcattc ttgccagtat gatcgtgaac
    21721 tggctgaaca agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg
    21781 cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg
    21841 attgcacgcg gataatattc tgcggaatgc gcagcacaga gtgaagacgt gacggattcc
    21901 ggaacagaaa aacagtcgta gaatcagcgg caagccggaa aggttccggt gaggcgcaat
    21961 gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt
    22021 gcaggtatgc acctgacaac atcacggggg ctaatcttga acccaaacaa ctcaagaata
    22081 gccgcgaaac gttggcatta caacacaggc ggctatatga cgctcgcaga actgggcatg
    22141 gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg
    22201 aactggctga ataagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat
    22261 ttgcgcccgg atcaggccgc agaggttaaa ctctgcggcc tttttcgtgc ttactgcggg
    22321 taaggcaccc agtcgccgcc gttcaggcga acgtacggtt tatcttggta ttgaataact
    22381 actgcatttg agttctcgga gaccggtgct gtttgtggca acccactggt gagttttttc
    22441 cagtcaacat tgtcttcggt gaaaatcttg ccatcaagaa cgcgaaccac cagatcggag
    22501 atagccagga agctgctcgg ttggtcaatg acaatcggtg ccccctgatg tggtgccttc
    22561 atgccgaaga atttcacccc aacggggacg tctgtgatag acgggctagg gatatcacgt
    22621 aggccagata cctgcattct gtcgcccttc agcgcgccgc cgtgttccgg caccacgacc
    22681 accatcactt tacgacccga tttctccagt tcagtaaaga aggcgtccag ttcatcaaag
    22741 aatttctgcg cccgcgcttt gtaatccgct gttttgctga cccccggata atggttgccg
    22801 tcatgcagtg gaagcgtgtt gtagaacgtg gcactacggc tgtttttatc tttttcggta
    22861 acgtccagcc agcggttaag cacggcggta tcgtcataaa ccggcgaacc atcaaagccc
    22921 agcaaaataa ccggcagatt tgtttgatcc atcaattcag tctgcatgcc gccattttcg
    22981 cgaacttctt tcaaaaaacc gccgaactgg ccgttatgtc ccatcatcag gtgctgggta
    23041 aagcccagtt tcgaaaggtt atcaaacaga tagcagtcgt tatttgccgg ttgatacaga
    23101 ttagtgtgcg aagtctgccc gcagctggcg cgcagtaaac ggatcgccgc cgggccactg
    23161 taggaggtgg cggagttaaa gttcttgaaa tcaatatcga aatgcgacca cagtggatgc
    23221 gacatcaacc cggcggcttc tatatccgac caggaaagcg aacagatgtt aatcaccagt
    23281 agttcaaatg gctgagcatc agcgggcagc gaagacggga aggtcgattt acgtttcgcc
    23341 tccgcgttat agaaattatt cagccaggcg ttaaggttcg ccgttgttgg cggtgcagtt
    23401 tgtgcgggca tatcacccac taccggtgcg ccacccgtcg ccgcaacggt tgccgctgcg
    23461 ttaccacccg tcgttgttac agtggtcgtc ggttgtccgg ctggccacaa ggagaaactt
    23521 ggtcccgcca aggtaagtac gttcagccat agcagtatgg caaccacaaa aacggtaatg
    23581 cgaatccatt gtgacaggaa taaccaggcc actaataaaa caaaaatggc cccaatcatc
    23641 tgccagttaa taaagcgtgt gacaaggtcg attaaataat cggtactgaa ccccgccacc
    23701 tgcgaaccct ggctcattat gctttccggg ccaggcaacc agatgtcatg ccagaacaaa
    23761 gcaaagccga tcggcagggc aatccagtgg cgcaagcgat gcaggctgta acgcggaatg
    23821 ggcatcagca gaaacgcggc aaacaccaaa ttgaggagcg ggtggaagtt aagatatccc
    23881 gcccacaaca ggccaaactt aaccagaaaa tagaagttcc agccggaaag gccgcgccag
    23941 tattgccaga gggaagaagg catggcggta ttttgcgtaa attgagtcat tttttggttg
    24001 ccctggcttt ttccgtgcgg cgtaacgtcc cggccggttt aacataacga ggtttagcaa
    24061 agaacaaacg taaggtgtcg cgaatgcgtc gcaaagagtg ccgcgccaga tagcccagcg
    24121 ggaaaaatat cagtgcgcaa acgataataa tttcaatgat atcgctgatg gtcatcatga
    24181 tgagcgctcc acagcatcat ctaacagtcg catgggttct ggtattcgtc gccagtggcg
    24241 accatcgtgc tcggcattga tgaccggttt agaactttgc gttaaaggca gcggcattcc
    24301 ccattgttct ggggcaagca ggcgcatctg caccagctcg gcactgattt gatcatcttc
    24361 aaaccagacc atacggtttg agaaaatgtc gccagtaggc aatgggaaaa tatgattcaa
    24421 cgcggtatcc agatcgttaa tccgacagaa tgagagaaac agcaccagcc gattaccgcc
    24481 aatggtcatg atatcgccgg tacggttagg gcgacatagc gtcagggctt gctcaacgcg
    24541 gatacccggt accggacgta gggcaaccag aacgccttta ccgtgggcag gcaagagagg
    24601 gttattcatc atgttgttga cggcattaca gaacacatcc cacttctgga aaccacgcag
    24661 tttgagcggc tgggtcattg acagcaaggt agtgatatct tccggtacat agcgactaaa
    24721 cttctgccct tgcacgcttt cgatcatcgt cagacaacgg gagagtggcg cattccacgg
    24781 aataaccata tttgcaccgc aggccaataa caaacgttca tcggtggcgc gcaggctagc
    24841 ggtattttcc cgcacgagga ttttcatcgc actaccgcgc tggcgacgca gggtatgaat
    24901 gctgcgggcc agtggctcga tttgcgcatt ttgctgtaaa gaaaagacca ccgtcgccgc
    24961 ctgagcggta cgggcttcat tgaacaggac ttcgttattg ttgaacagtt gccagtgttc
    25021 cgatagcggc ggcgcacctt ccagtacagc aacattactc agaatgcgtt tttcgtcgct
    25081 gcgtggttgg atctccgcct cttcgctttg aactaatgtc cagataccat tttgttgctg
    25141 aacgctaagc tgctgacggg cgctgacccc tttttcgttg caccagaagg caatatccag
    25201 caaatgttgg tcaccctgaa aacgcaaact ggcaagacca aaaagtgaac ggtattcctc
    25261 aagcaataat gaaaattgtt tatcgttatt atttccggga ttaattacca aaagcgaaca
    25321 atggtttaac ctgctccatt tattcatttt atccaaccat gagcgaagcc gctcggcagg
    25381 aatattttgc catgcgttat ttgcgcaaac aagaataaaa aggtaattat ggggatcaat
    25441 agaacactgc aaatcacggg ccaaatagta tagacctttt tcatgattta gcattgaaaa
    25501 taattttatt ttttccggcc cttgagaatc atctaattga aagattttcg ccggatcgct
    25561 gtccatgcta atgaccgcga cgtgtgcggt ttcagcctgg gatgcaattg tttgattcgc
    25621 cagactgata gcatcttcat ggcgatcgac gttaaaccac cagacgccgc ctgctggcat
    25681 atgtcgcagc tcatcccata atgatgagat accgatagag aatacagggt ccacaatgtc
    25741 cctcatgtca ccattttgtc tgattatcag tttactagcg aaagctaaga aataaaccta
    25801 ccattgaaat taaaacttat cagatttagc atgtgaatca atttagaagg tcacaagacg
    25861 tgtgatttta gtcttttcgt ttttaacttt ttctgttgcg taacggaaag tcaaaaagtg
    25921 agcaaattcc cgtctcgccg taaacaataa accggcgaaa tgctcacggt tagaattgtc
    25981 tcatgaatgg tacggttatt tcatagggat caagcaaaat gaataacaat gaaccagata
    26041 ctctgcctga tcccgcgata ggctatatct tccagaatga tattgtggcg ttaaagcagg
    26101 cattttcact gcctgatatt gattatgccg atatttccca acgcgaacag ttggccgcgg
    26161 cattaaaacg ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg attggtgaat
    26221 ggccgtactg ggattgcagg gggtgcgggg aggcgtgggg acaacaacca tcaccgccgc
    26281 attagcctgg tcattacaaa tgttgggaga aaatgtcctg gtggtcgatg cctgcccgga
    26341 caacttgttg cgcctgtcat ttaatgttga ttttacccac cgtcagggct gggccagagc
    26401 gatgctggat ggccaggact ggcgtgacgc tgggttgtgc tacacctcgc agctcgattt
    26461 gctgcctttt ggtcagttat ccattgaaga acaagaaaat ccacagcact ggcaaacccg
    26521 gctgagcgat atttgctccg gcttacagca actaaaagcc agcgggcgtt accagtggat
    26581 tttaatcgac ttaccgcgtg atgcctcgca gataacccac cagctgctga gtttgtgcga
    26641 gcactcgctg gcaatcgtca atgtggatgc caactgccat atccgactgc atcagcaagc
    26701 gctgccggat ggcgcacata ttttgattaa tgacttccgt attggcagtc aggttcagga
    26761 cgatatttac cagctttggt tgcaaagcca gcgccgatta ctgccgatgc tcattcatcg
    26821 tgatgaagcg atggctgaat gcctggcggc taagcaacca gtaggtgaat atcgcagtga
    26881 tgcgctggcg gctgaagaga tactgacgct ggcgaactgg tgcctgttga actactccgg
    26941 gctgaaaacg ccagtcggga gtgcatcatg agtatcctga cccggtggtt gcttatcccg
    27001 ccggtcaacg cgcggcttat cgggcgttat cgcgattatc gtcgtcacgg tgcgtcggct
    27061 ttcagcgcga cgctcggctg tttctggatg atcctggcct ggatttttat tccgctggag
    27121 cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc tgtatacgca tatcaacgcc
    27181 tcgcgtccgc gtccgctgga cccggtccgt tatctcattc aaacatgctg gttattgatc
    27241 ggtgcatcgc gcaaagaaac gccgaaaccg cgcagcgggc attttcaggt ctgcaaaata
    27301 ttcgtggacg ttaccatcaa tggatgaacg agctgcctga gcgcgttagc cataaaacac
    27361 agcatctgga tgaaaaaaaa gagctcggtc atttgagtgc cggggcgcgg cggttgatcc
    27421 tcggtatcat cgtcaccttc tcgctgattc tggcgttaat ctgcgttact cagccgttta
    27481 acccgctggc gcagtttatc ttcctgatgc tgctgtgggg ggtagcgctg atcgtacggc
    27541 ggatgccggg gcgcttctcg gcgctaatgt tgattgtgct gtcgctgacc gtttcttgcc
    27601 gttatatctg gtggcgttac acctctacgc tgaactggga cgatccggtc agcctggtgt
    27661 gcgggcttat tctgctcttc gctgaaacgt acgcgtggat tgtgctggtg ctcggctact
    27721 tccaggtagt atggccgctg aatcgtcagc cggtgccatt gccgaaggat atgtcgctgt
    27781 ggccgtcagt ggatatcttt gtcccgactt acaacgaaga tctcaatgtg gtgaaaaata
    27841 ccatttacgc ctcgctgggt atcgactggc cgaaagacaa actgaacatc tggatcctcg
    27901 atgacggcgg catggaagag tttcgccagt ttgcgcaaaa cgtgggggtg aaatatatcg
    27961 cccgcaccac tcatgaacat gcgaaagcgg gcaacatcaa caatgcgctg aaatatgcca
    28021 aaggcgagtt cgtgtcgatt ttcgactgcg accacgtacc aacgcgatcg ttcctgcaaa
    28081 tgaccatggg ctggttcctg aaagagaaac agctggcgat gatgcagact ccacaccact
    28141 tcttctcgcc ggacccgttt gaacgcaacc tgggacgttt tcgtaaaacg ccgaacgaag
    28201 gcacgctgtt ctatggtctg gtgcaggatg gcaacgatat gtgggacgcc actttcttct
    28261 gcggttcctg tgcggtgatt cgtcgtaagc cgctggatga aattggcggc attgctgtcg
    28321 aaaccgtgac tgaagatgcg catacttctc tgcgtttgca ccgtcgtggc tatacctccg
    28381 cgtatatgcg tattccgcag gcggcggggc tggcgaccga aagtctgtcg gcgcatatcg
    28441 gtcagcgtat tcgctgggcg cgcgggatgg tataaatctt ccgtctcgat aacccgctca
    28501 ccggtaaagg actgaagttt gctcagcggc tgtgctacgt caacgccatg ttccacttct
    28561 tgtcgggcat tccacggctg atcttcctga ctgcgccgct ggcgttcctg ctgcttcatg
    28621 cctacatcat ctatgcgcca gcgttgatga tcgccctatt cgtgctgccg catatgatcc
    28681 atgccagcct gaccaactcg aagatccagg gcaaatatcg ccactctttc tggagtgaaa
    28741 tctacgaaac ggtgctggcg tggtatatcg caccaccgac gctggtggcg ctgattaacc
    28801 cgcacaaagg caaatttaac gtcaccgcca aaggtggact ggtggaagaa gagtacgtcg
    28861 actgggtgat ctcgcggccc tacatcttcc ttgttctact caacctggtg ggcgttgcgg
    28921 taggcatctg gcgctacttc tatggcccgc caaccgagat gctcaccgtg gtcgtcagta
    28981 tggtgtgggt gttctacaac ctgattgttc ttggcggcgc agttgcggta tcggtagaaa
    29041 gcaaacaggt acgccgatcg aaccgcgtgg agatgacgat gcccgcggca attgcccgcg
    29101 aagatggtca cctcttctcg tgtaccgttc aggatttctc cgacggtggt ttggggatca
    29161 agatcaacgg tcaggcgcag attctggaag ggcagaaagt gaatctgttg cttaaacgcg
    29221 gtcagcagga atacgtcttc ccgacccagg tggcgcgcgt gatgggtaat gaagttgggc
    29281 tgaaattaat gccgctcacc acccagcaac atatcgattt tgtgcagtgt acgtttgccc
    29341 gtgcggatac atgggcgctc tggcaggaca gctacccgga agataagccg ctggaaagtc
    29401 tgctggatat tctgaagctc ggcttccgtg gctaccgcca tctggcggag tttgcgcctt
    29461 cttcggtgaa gggcatattc cgtgtgctga cttctctggt ttcctgggtt gtatcgttta
    29521 ttccgcgccg cccggagcgg agcgaaacgg cacaaccatc ggatcaggct ttggctcaac
    29581 aatgatgata acgcgatgaa aagaaaacta ttctggattt gtgcagtggc tatggggatg
    29641 agtgcgttcc cctctttcat gacgcaggcg acgccagcaa cgcaaccact gatcaatgct
    29701 gagccagctg tagccgccca gacggaacaa aatccgcagg tggggcaagt gatgtcaggc
    29761 gagcagggag ctgatgcccc catcgtggcg cagaacggtc cttcgcgtga tgtgaagctg
    29821 acctttgcgc aaatcgcgcc gccgccgggc agcatggtgc tacgtggtat taacccgaac
    29881 ggcagcattg agtttggtat gcgcagcgat gaagtggtga cgaaggcgat gctcaacttc
    29941 gaatacacgc catcgccatc gttactgcct gtccagtcgc agttaaaggt ttatctcaat
    30001 gatgagctga tgggcgtgct gccagtgacc aaagaacagt tgggtaaaaa aacgctggcg
    30061 caaatgccca ttaatccact gtttattacc gacttcaacc gtgtgcggct ggagtttgtc
    30121 ggccattatc aggacgtgtg cgaaaacccg gccagcacca cgctttggct ggatgttggg
    30181 cggagcagtg gactggatct gacctatcag accctgaatg tgaagaatga cctgtcacac
    30241 ttcccggtgc cattctttga cccgcgcgat aaccgtacta acaccttgcc gatggtcttt
    30301 gcgggtgcgc cggatgttgg gctgcaacaa gcatctgcca ttgtcgcctc gtggtttggt
    30361 tcgcgttctg gctggcgtgg gcagaacttc ccggtgctct ataaccaatt gccggatcgc
    30421 aatgccattg tctttgcaac caacgacaaa cggccggact tcctgcgcga tcatccggcg
    30481 gtaaaagccc cggtgattga gatgattaac catccgcaga atccttacgt caaactgctg
    30541 gtggtgtttg gtcgtgacga caaagacctg ttgcaggcag cgaaaggtat cgctcagggt
    30601 aacattctgt tccgtggtga aagcgtggta gtgaatgaag tgaaaccgct gctaccgcgt
    30661 aagccgtacg atgcgccgaa ctgggtacgt accgatcgtc cggtcacatt tggcgaactg
    30721 aaaacctatg aagaacagtt acaatccagc ggccttgagc cagcagcgat taacgtttcg
    30781 ctaaacctgc cgccggatct ctacctgatg cgcagtaccg gcattgatat ggatattaat
    30841 taccgctaca ccatgccgcc ggtgaaagac agttcgcgga tggatatcag cctgaataac
    30901 cagttcctgc aatccttcaa cctgagcagc aaacaggagg cgaaccgtct gctgctgcgt
    30961 attccggtat tacaaggttt gctggatggc aaaacagatg tctctattcc ggcgctgaaa
    31021 ctgggcgcga ccaaccagct gcgcttcgac tttgagtata tgaacccgat gccaggcggt
    31081 tcggtagata actgtattac cttccagccg gtgcagaatc atgtggtgat tggtgacgat
    31141 tccaccatcg acttctcgaa gtattaccac ttcatcccga tgccggatct acgcgccttt
    31201 gctaacgcgg gcttcccatt cagccggatg gcggatctgt cgcaaaccat caccgtgatg
    31261 ccgaaagcgc ctaacgaagc acagatggaa acgttgctga atactgttgg ttttattggc
    31321 gcacagacgg gcttcccggc gattaatctg acggtgaccg atgatggcag caccattcag
    31381 ggcaaagatg ccgacatcat gatcatcggt ggtatcccgg acaaactgaa agacgataag
    31441 cagatcgacc tattggtgca ggcgaccgaa agctgggtga aaacaccgat gcgccagacc
    31501 ccgttccccg gcattgtgcc ggacgagagc gatcgcgcgg cagaaaccca gtcaactctg
    31561 acctcttccg gtgcgatggc ggcggtgatt ggcttccagt cgccgtataa cgaccagcgc
    31621 agcgtgattg cgctgttggc agatagccca cgcggttatg aaatgcttaa cgatgcggtg
    31681 aacgatagcg gcaaacgcgc caccatgttc ggttcggtcg cggtgatccg cgagtccggt
    31741 atcaacagcc tacgtgttgg cgacgtttat tacgtaggtc atctgccgtg gttcgagcgc
    31801 ttgtggtatg cgctggcaaa ccatccgatt ctgctggcgg tgctggcgac tatcagtgtg
    31861 atattgctgg catgggtact gtggcgtctg ctgcgaatta ttagtagtcg tcgtcttaac
    31921 ccggataacg agtaattgaa gatgaatgtg ttgcgtagtg gaatcgtgac gatgctgttg
    31981 ttggctgcct ttagtgttca ggcagcctgt acctggcctg cctgggagca gtttaaaaag
    32041 gattacatca gtcaggaagg gcgcgtcatc gaccccagcg acgcgcgcaa aatcaccacc
    32101 tccgaagggc aaagttacgg tatgttcttt gccctggcgg ctaacgaccg tgcagctttc
    32161 gataatattc tcgactggac gcagaacaat ctcgctcagg gttctttaaa agaacgtttg
    32221 cccgcctggc tgtggggcaa gaaagagaac agtaagtggg aagtgctgga cagcaattcg
    32281 gcctccgatg gtgatgtctg gatggcctgg tcgttgctgg aggcggggcg tttgtggaaa
    32341 gagcagcgtt ataccgacat cggcagcgcg ttgctaaaac gtatcgcgcg ggaggaagtg
    32401 gtgacggtgc ctgggctggg ttccatgttg ttaccgggca aagtgggttt tgctgaggat
    32461 aacagctggc gttttaaccc cagctacatg ccgccgacgc tggcgcagta tttcacccgc
    32521 tttggcgcgc cgtggaccac gctgcgcgaa accaatcaac atttattgct ggaaaccgcc
    32581 ccgaaaggct tttcgccaga ctgggtgcgc tatgagaaag acaaaggctg gcagctaaaa
    32641 gccgaaaaaa cattgatcag cagctacgac gctatccgcg tttacatgtg ggtaggcatg
    32701 atgcctgaca gcgatccgca aaaagcgcgg atgctcaacc ggtttaaacc gatggcgaca
    32761 ttcactgaga aaaacggtta tccgccggaa aaagtggatg tggctacggg gaaagcgcag
    32821 ggtaaaggac cggtcggttt ttctgccgcc atgctgcctt ttttacaaaa ccgcgatgcg
    32881 caggcagttc agcgccagcg cgtggccgat aactttcccg gcagcgatac ctattacaac
    32941 tatgtgctga ccctgtttgg acaaggctgg gatcaacacc gtttccgctt ctcgacaaaa
    33001 ggtgagttat tacctgactg gggccaggaa tgcgcaaatt cacactaaac atattcacgc
    33061 tttccctcgg tctggccgtc atgccgatgg tcgaggcagc accaaccatc agcagaccct
    33121 gcggatggac agcggcaaca ctaatgccgt gcgcgggctg gcgaatattt accgccagca
    33181 gtcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagccagc ggcgcagcat
    33241 tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag aggtactgga
    33301 aaaccagggc aaatgggcgc aggcggcagc acttcagcgg caacgactgg cgctgggccc
    33361 cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg gacaacgcag
    33421 ccaggccgat acgttaatgc gcaatctggc gcagcagaag ccgaacaacc cggagcaggt
    33481 ttacgcttac gggctgtacc tctcaggtca caaccaggac agagcggcgc tggcgcatat
    33541 caacagcctg ccgcgcgcgc agtggaacag caatattcag gagctggtta atcgactgca
    33601 aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag aggctgaagc
    33661 ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc tggctgactg
    33721 ggcgtaacaa cgacgtgatt acaccgccgc ccgcactgca tatcagaatg tcctgacgcg
    33781 ggagccaact aacgccgatg ccattcttgg tctgacggaa gtggatattg ctgccggtga
    33841 caaagcggcg gcacgtagcc agctggcgaa actgcctgcc accgataaca cctcgctgaa
    33901 cactcagcgg cgcgtggcgc tggcacaggc gcagcttgga gataccgcag cggcgcagca
    33961 gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat
    34021 ggtactgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc aggcgctgga
    34081 aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc aggataacga
    34141 cacctttacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac gcggcgtgcg
    34201 cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcacccttg agcacgatta
    34261 ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta ccatgttgca
    34321 ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg tcaatatgaa
    34381 cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg gcacctgtac
    34441 attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg tggcggtcgg
    34501 ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct tcaacgtggt
    34561 ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt acaccgttaa
    34621 cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa aagactcccc
    34681 gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc taagtctgag
    34741 ctacgataaa ggtgaagtaa acggcgtctg ggcatcgctt agtggcgacc agttaaccgg
    34801 taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact ataaggtcat
    34861 taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc attacgacaa
    34921 agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg aatacctgtc
    34981 gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg agctgggggc
    35041 gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc tgatgaatct
    35101 gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg gcagcagtca
    35161 gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca actggtttgt
    35221 tggtacggca attgatatcc agcaggcgaa agattacgca cccagccatt tcctgctcta
    35281 cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc agccgctgat
    35341 accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct gcgatcgggt
    35401 atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt
    35461 aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt ttattttttg
    35521 caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta cgcaactgga
    35581 aagcattgct cgctctgtcc gcgaaccctt atcttcagct attttgaaag gcgatattcc
    35641 cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc gtgccgatgt
    35701 agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc gtccggtgcc
    35761 ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg tctactcgct
    35821 cgaacgtccg gcaaatccgc agccaatagc ctatctggtg ctacaggcgg attccttccg
    35881 tatgtataag ttcgtgatga gcactctctc aacgttagtg accatttact tacttttgtc
    35941 gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc atccgttacg
    36001 caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc accaactggc
    36061 attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa
    36121 ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc gcttcccggt
    36181 gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa
    36241 acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct
    36301 gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca aatcggtact
    36361 gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca ttgccaacgg
    36421 tgtacaggaa ccgtggcacg caatcacttt gggtcagcaa gtactcacta tcatgagcga
    36481 gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg tggcgatgtt
    36541 ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg catttaccgc
    36601 tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg aagcagccca
    36661 gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc agtttgcaat
    36721 ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg aagtgttact
    36781 gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg atcgcattga
    36841 gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct
    36901 tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacttct ctgcgctgca
    36961 actgatgcac ccgaatatgg tggcggatat gctggaactg ttaacccgct atcgcattca
    37021 gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc ctcatgctgc
    37081 ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg
    37141 catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact
    37201 gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga ttgctgcaat
    37261 tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg agactgaagc
    37321 acaacgagac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc tttttgctcg
    37381 cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc taccccaaac
    37441 tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt tttaaccttt
    37501 ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc
    37561 gcaggtaccc ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa
    37621 acctctctgt ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc
    37681 cttggccatt tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt
    37741 aagctcatta agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg
    37801 ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa
    37861 attgtcagta ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt
    37921 gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat
    37981 caggcgaaag accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt
    38041 ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt
    38101 gcgctccacc gtctgggcag caaaggccaa ctgattttta acgtgatcga aagtttctcg
    38161 caggtcatct tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcgttcggg
    38221 gcaatggcgt ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg
    38281 attatctgtt tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct
    38341 aaagcgactg gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt
    38401 gtactgggga cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa
    38461 ctcggctgcc gtaaatcggt ggtggggctg gttatcccga caggctactc gtttaacctt
    38521 gatggcacat cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt
    38581 cagatggata tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg
    38641 gcggcagggg taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc
    38701 catttgccgg tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct
    38761 cgtgcgctaa ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg
    38821 aaagaactgg accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa
    38881 acgcacgaat tatcctctta atctcaccac ttatgcccgc actcccttcc ccgagtgcgg
    38941 gtgtttgcgc ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat
    39001 ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc
    39061 atttagactg accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca
    39121 catgcagggc ataaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg
    39181 ctatgtgcag gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa
    39241 cggtttacag tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg
    39301 cctgctggtt aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc
    39361 catccctcgt attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt
    39421 gtggcagcag gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cttatgacac
    39481 cacgctgttt aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aagcgctctc
    39541 ttatctggca aatgccactg gcaaattgac catcacacca gaaaccatca accacgcgct
    39601 gcaaagtcag gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgcta
    39661 tcgtctgaaa gggtcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt
    39721 tgaagcggaa aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct
    39781 actggtggtg ggaaacgtgg atgcgcgctc ggttgtcgac caaatcaaca aaacgtttgg
    39841 cgaactgaaa ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc
    39901 ggaagcggtg agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga
    39961 tacgccgtgg cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct
    40021 ggcccgcgag gcgctgttct ggcatgttca gcaagcgtta agcgccagtg acagcaaaga
    40081 catcggtctt ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat
    40141 cgaatcacca aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa
    40201 ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact
    40261 ggagctgcag aaactgtttg ccgcctatgc acgggctgat accgatattc tgatgggcca
    40321 gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact
    40381 gcggcaggat ttccttaaca gcctgactgt ggagatgtta aatcaggatc tgcgtcagca
    40441 gttgtcgaat gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat
    40501 gaaagcgttg caggcggcct gggatcaaat catggcccca tctaccgccg ctgcgaccac
    40561 ctctgtcgcc acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg
    40621 cctgatggtg atgagttagc acaaccatca ggtatttatg gatgagctgg atagaggggt
    40681 aacactttat ccagcctttt gcatatgctg cgtttacgct ggcatcgcct cacgcgggat
    40741 aatcgcgccg cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt
    40801 ttccgcactg ccgcccgtca aacgtaccgc cagataaccg gcgctgaaag agtcgccagc
    40861 tgcggtggta tcgatcactt tttctttcgg cagtttcacc gccggaacat caactaacgc
    40921 ttcaccagca atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac
    40981 gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc
    41041 ttcgtcgtcc agcgtcagga aggcgatatc cgtgcattcc agcatttgct ggtacacctg
    41101 ctgtgtctct tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt
    41161 tccgcctttg gcgcggcatt cgcgcagcag ggaaagcagc ttttcgcggc tggtcgggct
    41221 taagatcgcc aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca
    41281 aatcgccgca gactgctcgc tcgccagcca gaatttggcg gcggcttcgt tacgccagta
    41341 gtagaacgta cgttcgccgg tgctgtcggt ttcaatgtag taaaggcccg gaagacggtt
    41401 ttccatccgt tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatctg
    41461 ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc
    41521 gacctgacgg gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac
    41581 gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga
    41641 catagtcgtg aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg
    41701 gtcaaccata ttaaaacagt gttccattat ttttttgagc cagaacgtat tcctgaaaga
    41761 ttccgttgtg gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttacgcgc
    41821 cgataatctt tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata
    41881 aggcaggtta tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg
    41941 cgtttttggt tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcggg
    42001 cggttaatgg ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaacgc
    42061 ctgccgccgg atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa
    42121 gagcagattg atttgctggc gcaaaaagcc gacttctttg tagagcacgg cctgctggca
    42181 tcggtcaata ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc
    42241 cagattgagc gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa
    42301 gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggtacc
    42361 gggatggcaa atttctctgc actaagtgaa gtgcgttatg actacatcaa aatcgcgcga
    42421 gaactgtttg tgatgctgcg tcagtcgccg gaaggccgca cactcttttc tcagctttta
    42481 catctaatga atcgctattg tcgcggggtg attgtcgagg gcgtagaaac accggaagag
    42541 tggcgtgatg ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg
    42601 gcaccgatgg aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc
    42661 gggctggact atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag
    42721 gcaggcaaaa taaccgctgc gatttcaggg gctttcttgt tgttgattgt cgtggcgatc
    42781 attttgattg caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct
    42841 gcggagttga atcgtccgtt cgctatccgt ggcaatctgg gcgtggtgtg ggagcggcaa
    42901 aaacaagaaa ctggctggcg cagctgggtg ccgtggcctc atgtacatgc agaagacatc
    42961 attcttggta atccaccgga tattcccgaa gtcacgatgg tgcatttgcc gcgagtagag
    43021 gcaacgctgg caccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc
    43081 gaaaagcccg acgcgcgcct gattcgcctc tctgaaaaga acaataactg gacgtttaat
    43141 cttgccaacg atgacaacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg
    43201 gataatattc ttttcgatca agggcggatc gccattgatg acaaagtaag caaagcggat
    43261 ctggaaattt ttgttgatcc gttaggcaag ccgctgccat tcagcgaagt tactggatcg
    43321 aaaggtaaag cggataaaga aaaggtgggc gattacattt ttggcctgaa ggcgcaggga
    43381 cgttataacg gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt
    43441 ggcgaaggga cgccgtttcc ggtacaggct gatttccgtt caggtaatac ccgtgttgct
    43501 tttgatggcg tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt
    43561 tctggcgatt cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg
    43621 ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt
    43681 gattatcgcg gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac
    43741 accaccggaa agccacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg
    43801 gcggacctgg gaccgttgat tggcgttgat tccgggaaag gggcagaaaa gtcgaaacgg
    43861 tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac
    43921 cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc
    43981 attgagcatg gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat
    44041 gctgacctgc gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat
    44101 attcatctgg aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga
    44161 ctgaaactga aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg
    44221 aacggtgacg cggaactacg cggtagcggt aactcggtgg cggcgctttt aggcaacagt
    44281 aacggcaacc tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt
    44341 gttgggctga atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg
    44401 gtgaactgcg cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt
    44461 gctttcgata ctgagaacgc gttgattaat gttaccggca cggcaagttt tgcttcggaa
    44521 cagctggatt tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg
    44581 ccgctgtatg tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cggaccgctg
    44641 attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta
    44701 ctggcactga tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag
    44761 atgaagaagt gatgataatt gccggatgtc tgacatccgg caattaaaca gatttagatt
    44821 tttggcaaag tgtgctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    44881 atccttgtga attcaatata ttgtatggaa attcagtcct gataagcgta gcgcatcagg
    44941 cttttcacac ttattacaac gactgatgtc gcgtctcatg ggtcagcagc agggcgatta
    45001 acgtcaagcc agccatcgcc gccagatata accccaccgc acctaacccg tagttagcct
    45061 gcaaccaggc tgcgatatac ggcgcaacgg aagccccgag aatcgacgat acgttgtaag
    45121 agaacgatgc tccggtgtaa cgcacttctg tcggaaacag ctctggtaac agcgcaccca
    45181 tcggcccgaa ggtcagcccc atcagactta accccagcag caggaaggca aaaaccagaa
    45241 tcgggttgcc agaaccgagc agcgggttaa aggcgaacag cgcgaacagg atgatcagcg
    45301 tggtgatgat caccatgctt ttacggcgac caaaggcatc agccagtaat ccggcgactg
    45361 gcaccatcac gccaaaacca ataactgcca tcatcagcat ccacaacact tcgttacgcg
    45421 gcaggccaag cccaactggc gcggcggcgg tactgaaggt catagagtag accgtcatga
    45481 tgtaaaacag cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat
    45541 gtttggtcag cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct
    45601 caaacaccgg cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg
    45661 ccgagaagat aaatggcaca cgccagcccc agctcataaa ctgctcatcg gtcagtagcc
    45721 aggaaagcag caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cctagctgtg
    45781 ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg
    45841 caccgcccca ttcaccgcct aagcccagac cctgaccaaa tcgagccagc gccagcagca
    45901 gtggggcgaa aataccaatc gtggcatagc ccggcagcag accaatcacc acggtcgaaa
    45961 tccccatcgt tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat
    46021 gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg
    46081 actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa
    46141 cggccgcagt ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg
    46201 cgacaaggac tttattacgc gagttgatcg gcgtgtattc ttgttcgtgg tcgagtgttg
    46261 tggctgttgc ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac
    46321 agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac caggcaaaaa
    46381 ggggataatg tttaggccag tgatttttct tctatgaaat gcttcacata attttataaa
    46441 tagtggataa aactggtttc aggttaaata aaagttacca actggattta tatgctgggg
    46501 ttatgagttg gagtgaaatt gtttgctccc aaatagatgg ctgaagaggc tctggggaag
    46561 agtttcaaga ggttatgtgc gcattatagc cggatgcgat gctggcgcat cttatccggc
    46621 ctacggggag ccaggccgaa taaaatcgta tgccggataa ggcgtttacg ccgcatccgg
    46681 cctattttaa ggctgcgttt tccctggcat tcgcgggtcg tctttatatt cggcggtggc
    46741 aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg ccatcagccc
    46801 cagcacggaa ccaaatgctg cgccagaggg ggatttcatc agcgatggca gggtgtaggt
    46861 catcacgatt ttaatcactt caaaaccaat agcagccaga aatgttccgc gaatcagggc
    46921 ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa gcagatagtt
    46981 ggcgaagatg gaaatcgcca ggccaatcaa tcgccacgtc ggtttcagcc actcaatgct
    47041 gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa ccggcaaccg aggtgatcga
    47101 aagcgtcaca atcagcgcaa tcaacaaacc aatcagtgaa ataaaatcac gcagatattt
    47161 tacccagaac ttctcctgat cttgcggcga gcgttcccag acatcgcgcg actgggcacg
    47221 aatcgcttca cgcaggttac ccatccagtt gatgccggaa taaagtgcca ccgccaggcc
    47281 gacaagccct acagtcgtac gctgctgaac ggcggtgttg atggtgtttt tcaacgtggc
    47341 ggctagtgtc ggatcgctga tgttttgcag aattttgtcg aagatatcct gtagcaacat
    47401 cggatgggag gccagcacaa agccccctgc ggcaaacgac accatcaaaa tcgggatcat
    47461 cgacaaaaat gagaaatagg tgatagccgc accaaactgg ttacccagcc gatcgttaaa
    47521 gcgttctgtc gcgcgaataa ggtgcgcaat aaccggttgg cgttggactt tttcggcagt
    47581 ggtggtgacg gtttccagcg cctggctaac tttgccgcct ttctcgctat tatccaactg
    47641 cttaatcggc tcgtgctcca gatcctgggt gggacgtttg atctcgtttt cctgcgtcat
    47701 ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc agtccaggtg
    47761 ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg aaagctcccg
    47821 acgctgcggg taaaccagcg aaagggagag cggctcggca cggtagcctg gcaacacttc
    47881 aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct gaataatccc
    47941 cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca acatgccgcc
    48001 agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg gatgtacacc
    48061 caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc tttgcggata
    48121 accaaagcgc gtcagatagt gcggactggc acagttgacc atggttagtt tgccgagggg
    48181 acgggcgata acgccatctt ccggtaacgc gccagtgcgt atcacgcaat caaaaccatc
    48241 atgaagaata tctaccggac ggttatggct actcagttcc agctcaattc ccggatgctg
    48301 atagagaaat tccgacagac gcggcagtaa caggtttttc gcgattcctg acgggatgtc
    48361 gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt ccagttcgct
    48421 gagattactc aacacatctt ttgcccgttg ataataggtc atgccttctg gcgtgagttt
    48481 gacccgtcgc gtagtgcgct ggagaagctg ggtgccaagt tgatgttcca gtgcctgtat
    48541 ctggcgcgaa acacttccct ttggcaaagc aaagaaatcc gccgcgcggg aaaaactttc
    48601 cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt ctttgaccga
    48661 ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg tttattgttt tttctaacat
    48721 ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc gatttctcag caggaaaagg
    48781 gcggaatagc tttatgagaa aggtgaatcc ccttaaacag agcgtatttt tcagtgattc
    48841 cctttaaagt gaatagagga cgtattggct atttgtgctt atttttgtct cattgccatt
    48901 tgttattttg gcttgtagtc gcggaatttc atttttaatt gcatgcgtac taaccaccaa
    48961 tataaggaaa tcctcaaaat tgttttaaat agtgccctcg acatgtaatt tcaggcgttt
    49021 agctgtaact atttatatcg gtaactattt tctacgcgtc atctctgtag aatggccacc
    49081 gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac ttaagtcttt
    49141 ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag gcagtcaata
    49201 tttattcatg gaataaaaat cagtttacag cagcgtattc caggagtgag tattcagggg
    49261 gtcagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc cttagttatg
    49321 ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac cgtggtgcag
    49381 tttcctgagg ttaaggtgtt aatcacggcg acggattgca ataaacggtg gttacaggaa
    49441 gttatccatt ttaatgtgct ggccattgtg cctcgcgatt caaccgtcga gacatttgcg
    49501 ttggcggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg gcggacgacg
    49561 ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga gattttaacc
    49621 atgttagcgg cgggcgaatc aaataaggag atcggcagag cgctgaatat cagtaccgga
    49681 acggtaaaag cgcatctgga atccctgtat cgccgtctgg aagtgaaaaa tcgcacccag
    49741 gcggcaatga tgttaaatat ctcctcctg
//
LOCUS       DAEBXD010000019        49536 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEBXD010000019 DAEBXD010000000
VERSION     DAEBXD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49536
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.019"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KJL94_001096"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KJL94_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBD4409536.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVLLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="KJL94_001097"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="KJL94_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBD4409537.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="KJL94_001098"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="KJL94_001098"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBD4409538.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="KJL94_001099"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="KJL94_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBD4409539.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="KJL94_001100"
     CDS             5724..6665
                     /locus_tag="KJL94_001100"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBD4409540.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="KJL94_001101"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="KJL94_001101"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBD4409541.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="KJL94_001102"
     CDS             7685..8935
                     /locus_tag="KJL94_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD4409542.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KJL94_001103"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KJL94_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="KJL94_001104"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="KJL94_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="KJL94_001105"
     CDS             10872..12122
                     /locus_tag="KJL94_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD4409543.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="KJL94_001106"
     CDS             complement(12178..13266)
                     /locus_tag="KJL94_001106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBD4409544.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KJL94_001107"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KJL94_001107"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBD4409545.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="KJL94_001108"
     CDS             complement(14200..15249)
                     /locus_tag="KJL94_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBD4409546.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="KJL94_001109"
     CDS             15348..16229
                     /locus_tag="KJL94_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4409547.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="KJL94_001110"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="KJL94_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBD4409548.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="KJL94_001111"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="KJL94_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBD4409549.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KJL94_001112"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KJL94_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="KJL94_001113"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="KJL94_001113"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBD4409550.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="KJL94_001114"
     CDS             21997..23073
                     /locus_tag="KJL94_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBD4409551.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="KJL94_001115"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="KJL94_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBD4409552.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="KJL94_001116"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="KJL94_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBD4409553.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="KJL94_001117"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="KJL94_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBD4409554.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="KJL94_001118"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="KJL94_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBD4409555.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KJL94_001119"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KJL94_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KJL94_001120"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KJL94_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBD4409556.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="KJL94_001121"
     CDS             complement(30815..31054)
                     /locus_tag="KJL94_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBD4409557.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KJL94_001122"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KJL94_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBD4409558.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KJL94_001123"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KJL94_001123"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBD4409559.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="KJL94_001124"
     CDS             complement(32940..33635)
                     /locus_tag="KJL94_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBD4409560.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="KJL94_001125"
     CDS             complement(33632..34030)
                     /locus_tag="KJL94_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBD4409561.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="KJL94_001126"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="KJL94_001126"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBD4409562.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KJL94_001127"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KJL94_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBD4409563.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="KJL94_001128"
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="KJL94_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBD4409564.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37383..38144
                     /locus_tag="KJL94_001129"
                     /pseudo
     CDS             37383..38144
                     /locus_tag="KJL94_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="KJL94_001130"
     CDS             complement(38261..38839)
                     /locus_tag="KJL94_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4409565.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="KJL94_001131"
     CDS             39009..39596
                     /locus_tag="KJL94_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBD4409566.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="KJL94_001132"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="KJL94_001132"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBD4409567.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KJL94_001133"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KJL94_001133"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD4409568.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="KJL94_001134"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="KJL94_001134"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBD4409569.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46117
                     /gene="osmF"
                     /locus_tag="KJL94_001135"
     CDS             45200..46117
                     /gene="osmF"
                     /locus_tag="KJL94_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBD4409570.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46124..47281
                     /gene="yehY"
                     /locus_tag="KJL94_001136"
     CDS             46124..47281
                     /gene="yehY"
                     /locus_tag="KJL94_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBD4409571.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47274..48200
                     /gene="yehX"
                     /locus_tag="KJL94_001137"
     CDS             47274..48200
                     /gene="yehX"
                     /locus_tag="KJL94_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBD4409572.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48205..48936
                     /gene="yehW"
                     /locus_tag="KJL94_001138"
     CDS             48205..48936
                     /gene="yehW"
                     /locus_tag="KJL94_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBD4409573.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48917..49024)
                     /locus_tag="KJL94_001139"
     CDS             complement(48917..49024)
                     /locus_tag="KJL94_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBD4409574.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49084..49536)
                     /locus_tag="KJL94_001140"
                     /pseudo
     CDS             complement(49084..49536)
                     /locus_tag="KJL94_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11958 a        12201 c        13311 g        12066 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaggac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac agcttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagagccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attataccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ttgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat gccgacaagc
    44521 cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat ctttcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccgg
    45421 tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta aagatgaaaa
    45481 cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga
    45541 gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt
    45601 gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga gtcgttatct
    45661 gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac gcgccgatgc
    45721 gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt tgctgtcact
    45781 ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct ctggcgttaa
    45841 tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag
    45901 cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt
    45961 gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc tcgatgcaaa
    46021 aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca aaaaagtggc
    46081 tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt atctacgtat
    46141 taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc tgccgtttat
    46201 cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc aactgtggcc
    46261 gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc
    46321 cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt
    46381 gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac
    46441 cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct gtagcgatgc
    46501 catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc agattgccat
    46561 tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa tgaaagaata
    46621 cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt tgctgtttgg
    46681 tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct acttttccac
    46741 tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc cttcggtggc
    46801 gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc cgtggctggg
    46861 gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg tgctctatgc
    46921 cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct
    46981 ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc
    47041 gctggcgtta ctggtatttc tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat
    47101 ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt tccaggggct
    47161 gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg ttctggcggt
    47221 gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac gtaatgattg
    47281 aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca
    47341 attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc aaatccacca
    47401 ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc tttgccggag
    47461 aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat gccattcaat
    47521 ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac
    47581 aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg ctactggggc
    47641 tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag cagcaacgtg
    47701 tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat gaaccttttg
    47761 gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt
    47821 tgctggggcg cactattgtg ctggtcactc atgatattga tgaggcgcta cggctggcag
    47881 aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg ctgacgatgc
    47941 tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc
    48001 acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg
    48061 aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc gcgcggggat
    48121 gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg cattttcagg
    48181 atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt
    48241 gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt tgctgccttg
    48301 ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct ggcactggct
    48361 catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac aggtgccgga
    48421 attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac tattgccgcc
    48481 gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt
    48541 ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat cctgcaggtg
    48601 acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa aggtatggga
    48661 atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc ggtgattctg
    48721 gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc ctcaacggta
    48781 ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa taccgcgtat
    48841 gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg cctgtttgaa
    48901 aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg agcatgacgc
    48961 caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt ttagctatgc
    49021 gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt tgttaacgaa
    49081 tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg tgagttgctg
    49141 ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc cttcgaatag
    49201 ttcagggcgt agttgattga gcgagtgggc gagcgaggac atcaatgcgc catccttgtt
    49261 gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc cagccaacca
    49321 ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg atgtagttga
    49381 tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg cactgaagcc
    49441 gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa tcctgaccgc
    49501 gctctttgat ccacgttcgc aggctatgta gattgc
//
LOCUS       DAEBXD010000020        48607 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEBXD010000020 DAEBXD010000000
VERSION     DAEBXD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48607
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.020"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            30..1280
                     /locus_tag="KJL94_001141"
     CDS             30..1280
                     /locus_tag="KJL94_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4409575.1"
                     /translation="MSKLESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHK
                     RVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEY
                     FGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHR
                     MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLA
                     ASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLN
                     FFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHI
                     GGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETH
                     GIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1277..2242
                     /gene="yggF"
                     /locus_tag="KJL94_001142"
     CDS             1277..2242
                     /gene="yggF"
                     /locus_tag="KJL94_001142"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD4409576.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2264..2773
                     /gene="fumE"
                     /locus_tag="KJL94_001143"
     CDS             2264..2773
                     /gene="fumE"
                     /locus_tag="KJL94_001143"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBD4409577.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNETLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2770..3483
                     /locus_tag="KJL94_001144"
     CDS             2770..3483
                     /locus_tag="KJL94_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBD4409578.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3455..4137)
                     /locus_tag="KJL94_001145"
                     /pseudo
     CDS             complement(3455..4137)
                     /locus_tag="KJL94_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4131..4808)
                     /locus_tag="KJL94_001146"
     CDS             complement(4131..4808)
                     /locus_tag="KJL94_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBD4409579.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4796..5179)
                     /locus_tag="KJL94_001147"
     CDS             complement(4796..5179)
                     /locus_tag="KJL94_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBD4409580.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5195..5503)
                     /locus_tag="KJL94_001148"
     CDS             complement(5195..5503)
                     /locus_tag="KJL94_001148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409581.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5504..6082)
                     /locus_tag="KJL94_001149"
     CDS             complement(5504..6082)
                     /locus_tag="KJL94_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBD4409582.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6105..6536)
                     /locus_tag="KJL94_001150"
                     /pseudo
     CDS             complement(6105..6536)
                     /locus_tag="KJL94_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6908..7927
                     /gene="epd"
                     /locus_tag="KJL94_001151"
     CDS             6908..7927
                     /gene="epd"
                     /locus_tag="KJL94_001151"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBD4409583.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7977..9140
                     /gene="pgk"
                     /locus_tag="KJL94_001152"
     CDS             7977..9140
                     /gene="pgk"
                     /locus_tag="KJL94_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBD4409584.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9355..10434
                     /gene="fbaA"
                     /locus_tag="KJL94_001153"
     CDS             9355..10434
                     /gene="fbaA"
                     /locus_tag="KJL94_001153"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBD4409585.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10625..11485
                     /gene="mscS"
                     /locus_tag="KJL94_001154"
     CDS             10625..11485
                     /gene="mscS"
                     /locus_tag="KJL94_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBD4409586.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11624..12259
                     /gene="argO"
                     /locus_tag="KJL94_001155"
     CDS             11624..12259
                     /gene="argO"
                     /locus_tag="KJL94_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBD4409587.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12352..13092
                     /locus_tag="KJL94_001156"
     CDS             12352..13092
                     /locus_tag="KJL94_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBD4409588.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13259..14209
                     /locus_tag="KJL94_001157"
     CDS             13259..14209
                     /locus_tag="KJL94_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4409589.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KJL94_001158"
     CDS             complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KJL94_001158"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBD4409590.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KJL94_001159"
     CDS             complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KJL94_001159"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBD4409591.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KJL94_001160"
     CDS             complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KJL94_001160"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBD4409592.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KJL94_001161"
     CDS             complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KJL94_001161"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBD4409593.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KJL94_001162"
     CDS             complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KJL94_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBD4409594.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21052..21711
                     /gene="rpiA"
                     /locus_tag="KJL94_001163"
     CDS             21052..21711
                     /gene="rpiA"
                     /locus_tag="KJL94_001163"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBD4409595.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21967..23199
                     /gene="serA"
                     /locus_tag="KJL94_001164"
     CDS             21967..23199
                     /gene="serA"
                     /locus_tag="KJL94_001164"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBD4409596.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23428..23487
                     /gene="ibsC"
                     /locus_tag="KJL94_001165"
     CDS             23428..23487
                     /gene="ibsC"
                     /locus_tag="KJL94_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBD4409597.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23820..23879
                     /locus_tag="KJL94_001166"
     CDS             23820..23879
                     /locus_tag="KJL94_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD4409598.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23980..24582)
                     /locus_tag="KJL94_001167"
     CDS             complement(23980..24582)
                     /locus_tag="KJL94_001167"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBD4409599.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24603..24786)
                     /gene="ssrS"
                     /locus_tag="KJL94_001168"
     ncRNA           complement(24603..24786)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KJL94_001168"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KJL94_001169"
     CDS             complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KJL94_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBD4409600.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25325..25903
                     /gene="ygfB"
                     /locus_tag="KJL94_001170"
     CDS             25325..25903
                     /gene="ygfB"
                     /locus_tag="KJL94_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBD4409601.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25929..27254
                     /gene="pepP"
                     /locus_tag="KJL94_001171"
     CDS             25929..27254
                     /gene="pepP"
                     /locus_tag="KJL94_001171"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBD4409602.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27251..28429
                     /gene="ubiH"
                     /locus_tag="KJL94_001172"
                     /gene_synonym="visB"
     CDS             27251..28429
                     /gene="ubiH"
                     /locus_tag="KJL94_001172"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBD4409603.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28452..29654
                     /gene="ubiI"
                     /locus_tag="KJL94_001173"
                     /gene_synonym="visC"
     CDS             28452..29654
                     /gene="ubiI"
                     /locus_tag="KJL94_001173"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBD4409604.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29728..29811)
                     /locus_tag="KJL94_001174"
     CDS             complement(29728..29811)
                     /locus_tag="KJL94_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409605.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30102..31196
                     /gene="gcvT"
                     /locus_tag="KJL94_001175"
     CDS             30102..31196
                     /gene="gcvT"
                     /locus_tag="KJL94_001175"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBD4409606.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31220..31609
                     /gene="gcvH"
                     /locus_tag="KJL94_001176"
     CDS             31220..31609
                     /gene="gcvH"
                     /locus_tag="KJL94_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBD4409607.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31728..34601
                     /gene="gcvP"
                     /locus_tag="KJL94_001177"
     CDS             31728..34601
                     /gene="gcvP"
                     /locus_tag="KJL94_001177"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBD4409608.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34868..35610
                     /locus_tag="KJL94_001178"
                     /pseudo
     CDS             34868..35610
                     /locus_tag="KJL94_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KJL94_001179"
     CDS             complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KJL94_001179"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBD4409609.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37145..37455
                     /locus_tag="KJL94_001180"
                     /pseudo
     CDS             37145..37455
                     /locus_tag="KJL94_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37619..38278
                     /locus_tag="KJL94_001181"
     CDS             37619..38278
                     /locus_tag="KJL94_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBD4409610.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KJL94_001182"
     CDS             complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KJL94_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBD4409611.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFKHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39697..39963
                     /gene="sdhE"
                     /locus_tag="KJL94_001183"
     CDS             39697..39963
                     /gene="sdhE"
                     /locus_tag="KJL94_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBD4409612.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39944..40351
                     /locus_tag="KJL94_001184"
     CDS             39944..40351
                     /locus_tag="KJL94_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBD4409613.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KJL94_001185"
     CDS             complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KJL94_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBD4409614.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41024..41920
                     /gene="xerD"
                     /locus_tag="KJL94_001186"
     CDS             41024..41920
                     /gene="xerD"
                     /locus_tag="KJL94_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBD4409615.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41945..42655
                     /gene="dsbC"
                     /locus_tag="KJL94_001187"
     CDS             41945..42655
                     /gene="dsbC"
                     /locus_tag="KJL94_001187"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBD4409616.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42661..44394
                     /gene="recJ"
                     /locus_tag="KJL94_001188"
     CDS             42661..44394
                     /gene="recJ"
                     /locus_tag="KJL94_001188"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBD4409617.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44485..45583
                     /gene="prfB"
                     /locus_tag="KJL94_001189"
     CDS             join(44485..44559,44561..45583)
                     /gene="prfB"
                     /locus_tag="KJL94_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBD4409618.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45593..47110
                     /gene="lysS"
                     /locus_tag="KJL94_001190"
     CDS             45593..47110
                     /gene="lysS"
                     /locus_tag="KJL94_001190"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBD4409619.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KJL94_001191"
     CDS             complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KJL94_001191"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBD4409620.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47756..47827
                     /locus_tag="KJL94_001192"
     CDS             47756..47827
                     /locus_tag="KJL94_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409621.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47824..47949)
                     /locus_tag="KJL94_001193"
     CDS             complement(47824..47949)
                     /locus_tag="KJL94_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409622.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KJL94_001194"
     CDS             complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KJL94_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBD4409623.1"
                     /translation="IIVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTF
                     NSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAG
                     LVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLL
                     HSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE
                     "
BASE COUNT        11666 a        12185 c        13120 g        11636 t
ORIGIN      
        1 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccgaatttg
       61 aactgccaga aattaccgat aatgaactgt tagtgagtgt aatttctgac agcgtctgtt
      121 tatcgacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc gacgatttag
      181 aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa gtgggtaaaa
      241 atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg atggggttac
      301 caagcggata ttcagcgggc tacagctacg aatattttgg cggcaatgcc acttatatga
      361 ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc tcttattttg
      421 ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat gccaattatc
      481 acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc aatattgcac
      541 tgctggcgtg tgcgggtccg atgggcattg gcgctatcga ttacgccatt aacggtggca
      601 tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtgcaga
      661 agctgctgcc ggtggatctg gcggccagta aaggcattga gctagtgtat gtgaatacca
      721 aagggatgag cgatcctatc cagatgctgc gggcgctgac aggagatgtc gggttcgatg
      781 acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa ttactggcgg
      841 aagatggctg tctgaacttc tttgccgggc tgacggataa aaacttcaaa gtgccgttta
      901 atttctacaa cgtccattac aacagcacgc acgtagtcgg tacatccggc ggttcaacgg
      961 acgacatgaa agaggcgatt gcccttagcg ccacaggaca gttacagccg tcgtttatgg
     1021 tgacccatat tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc
     1081 ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc attgccgatt
     1141 ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa
     1201 cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt ggcgttgata
     1261 tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc
     1321 agcgttggct gcctggccgc acaccggatg tggcgacaaa aataaaattg atggcctggc
     1381 ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag tggttatcgg
     1441 cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg gcaaaggcga
     1501 tgggccagaa gtcgatatcg cggttgatcc cattgaaggc acgcggatgg tggcgatggg
     1561 gcagagcaat gcgctggcgg tcatggcttt cgctcctcga gatagcctgt tacatgcgcc
     1621 cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga ttgatctgtc
     1681 tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa
     1741 gctgcgcatg gttacgctgg ataaaccgcg tctgagtgcc gcgattgaag aagcaacaca
     1801 gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg tgctgacctg
     1861 ctggcaggat aatccttacg atgtgatgta caccatcggc ggtgcgccag aaggcgtgat
     1921 ttctgcctgt gccgttaaag cgttaggcgg ggatatgcag gccgagctga ttgacttttg
     1981 tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc gcaagcgttg
     2041 taaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg tgagggggaa
     2101 cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca
     2161 gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa
     2221 tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga
     2281 agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc
     2341 gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga
     2401 tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga
     2461 tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtatgc
     2521 cgatatcacg cacttttccc agtactggca ttacctgaac gaacaggatg aaacgcccgg
     2581 atttgccgat gatataacct gggattttat cagcaatgtc aacagtataa ctcgcaatga
     2641 gacgctctac gatgcgttaa aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc
     2701 gcgttttagc ggaatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga
     2761 attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc gcagtaccat
     2821 gatgaagaga tagaacgcgt gcataaactg ttgctgcgta tgctggcggc cttacagacg
     2881 gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac gggtaaatct
     2941 accctaacca ccttctggga atatctcgcg caacaagacc cggagctgcc cgctattcag
     3001 gcgctcccga tggatggttt tcaccattac aatagctggc tggacgcgca tcaattgcgc
     3061 cccttcaaag gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa tctgcgccag
     3121 gtggtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa
     3181 gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt gttgctggac
     3241 gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat caacgcgcca
     3301 gctgctgcgt tgcgggagcg tctggtgggg cgcaagctgg cgggaggggt atcgctggca
     3361 gatgcagaag ccttttatga ccgtaccgac gggccgaatg ttcgccgggt gctggaagag
     3421 agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg tctggtggat
     3481 taacgttcct gattacacca tcttccagcc gcaccacacg agagaaatga cgccgggtaa
     3541 atgcggcgtc atgactgatt gctaccacgc tcgtgcctcg ctggcggcac ttctccagcc
     3601 agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg tccagcagta
     3661 atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc tgcgcactat
     3721 gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg acaacattgc agggcagcat
     3781 gggtacgttg agtgatttca tccgccgggc atttttgcag tttcaaacca aatgcgatct
     3841 cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacaga acagtacgcc
     3901 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     3961 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
     4021 gccattgtca ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     4081 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     4141 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     4201 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     4261 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaag
     4321 agctttggct gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag
     4381 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     4441 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     4501 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     4561 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     4621 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     4681 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     4741 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     4801 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     4861 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     4921 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     4981 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
     5041 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     5101 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     5161 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
     5221 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     5281 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     5341 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     5401 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     5461 aggccgccaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     5521 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     5581 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     5641 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     5701 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     5761 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     5821 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     5881 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     5941 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     6001 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     6061 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     6121 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     6181 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     6241 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     6301 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     6361 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     6421 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     6481 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     6541 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     6601 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     6661 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     6721 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     6781 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     6841 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     6901 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     6961 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     7021 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     7081 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     7141 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     7201 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     7261 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     7321 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     7381 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     7441 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     7501 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     7561 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     7621 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     7681 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     7741 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     7801 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     7861 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     7921 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     7981 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     8041 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     8101 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     8161 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     8221 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     8281 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     8341 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     8401 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     8461 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     8521 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     8581 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     8641 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     8701 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     8761 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     8821 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     8881 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     8941 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     9001 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     9061 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     9121 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     9181 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     9241 gagtgaatcc ccaggagctt acataagtaa gtgactgggg ggagcgaacg cagacgcagc
     9301 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     9361 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     9421 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     9481 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     9541 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     9601 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     9661 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     9721 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     9781 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     9841 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     9901 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     9961 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
    10021 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    10081 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    10141 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    10201 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    10261 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    10321 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    10381 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    10441 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
    10501 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
    10561 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
    10621 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
    10681 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
    10741 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
    10801 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
    10861 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
    10921 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
    10981 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
    11041 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
    11101 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
    11161 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
    11221 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
    11281 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
    11341 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
    11401 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
    11461 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
    11521 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
    11581 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
    11641 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
    11701 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
    11761 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
    11821 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
    11881 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
    11941 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
    12001 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
    12061 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
    12121 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
    12181 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
    12241 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
    12301 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
    12361 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
    12421 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
    12481 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
    12541 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
    12601 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
    12661 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
    12721 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
    12781 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
    12841 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
    12901 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
    12961 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
    13021 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
    13081 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
    13141 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
    13201 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
    13261 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
    13321 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
    13381 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
    13441 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
    13501 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
    13561 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
    13621 gactgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
    13681 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
    13741 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
    13801 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
    13861 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
    13921 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
    13981 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
    14041 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
    14101 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
    14161 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    14221 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
    14281 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
    14341 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    14401 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    14461 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    14521 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    14581 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    14641 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    14701 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    14761 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    14821 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    14881 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    14941 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    15001 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    15061 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    15121 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    15181 caggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    15241 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    15301 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    15361 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    15421 tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    15481 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    15541 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    15601 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    15661 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    15721 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    15781 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    15841 cagagatgtg gtgcgccatt tgtaaggtga aatcttccac ttccacaaca tggttgagga
    15901 tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agctctttga
    15961 caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc
    16021 cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac
    16081 tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt
    16141 tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat
    16201 cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggatccac
    16261 ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt aacgcctgca
    16321 taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag ttaaactcaa
    16381 tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata aaaattcctt
    16441 taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag
    16501 agcgtattgt ttttgaccgc taaaagcgtc tggtggtaat agcgatcgaa atcttcattc
    16561 gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt
    16621 tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg
    16681 gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag accccgtggc
    16741 tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca
    16801 atggcgacat tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact
    16861 tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag
    16921 ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgacgcc aaccgtttcg
    16981 acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg tgctcgctga
    17041 ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc
    17101 gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg
    17161 ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat gccaaaggct
    17221 tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta
    17281 ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg acgtgggtga
    17341 cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg acgtaagcgg
    17401 cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac
    17461 gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc
    17521 ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc
    17581 gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc
    17641 cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc
    17701 aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc
    17761 gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg
    17821 gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga
    17881 ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg
    17941 caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg
    18001 aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt
    18061 cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag
    18121 cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc
    18181 ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag
    18241 cgactgttcg cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc
    18301 tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat
    18361 ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgtggcagag
    18421 ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt
    18481 aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt
    18541 cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag
    18601 gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc
    18661 aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat
    18721 aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt catcaatttt
    18781 cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgatcg taaatgctac
    18841 ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt
    18901 aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg
    18961 ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat cgttttgaat
    19021 ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc
    19081 cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat
    19141 ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac
    19201 gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc
    19261 aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt
    19321 tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    19381 acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc
    19441 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    19501 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    19561 ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    19621 tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc
    19681 cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa
    19741 cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga
    19801 ccataatcga gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg
    19861 tgccagtaga gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg
    19921 gccagctctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca
    19981 agttgtacga acgcttctga agaattaacg atatggcagg gtacgctgcc aggaggaaga
    20041 tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact
    20101 ggcgctttca ataatgccga acgcgttacg ccgttaggaa aatatttttc ggcaaacggt
    20161 tttgagctga cgaacagata gtcgagcgca ccgagtttat cgacaagaca actcggcagc
    20221 gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta
    20281 cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc
    20341 agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc
    20401 ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc
    20461 agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac
    20521 agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta
    20581 atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc
    20641 tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt
    20701 cctgcactat gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca
    20761 tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg
    20821 cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct ctgttcgcac
    20881 cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg
    20941 ccattgcgcg atattttatg gatgagttaa ccacccagct tgccaacggg gtctgaatcg
    21001 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    21061 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggtaccatt
    21121 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg cacgatgaaa
    21181 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    21241 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    21301 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    21361 aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc caagcaggtt
    21421 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    21481 gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    21541 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    21601 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    21661 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    21721 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    21781 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    21841 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    21901 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    21961 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    22021 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    22081 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    22141 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    22201 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    22261 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    22321 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    22381 tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    22441 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    22501 ttttatgata ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct
    22561 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    22621 aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc
    22681 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    22741 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    22801 tctccactgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    22861 gaagcgcagg agaatatcgg cttggaagtt gcgggtaaat tgatcaagta ttctgacaat
    22921 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt
    22981 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    23041 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    23101 gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    23161 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa
    23221 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    23281 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    23341 tatttaatgg ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc
    23401 gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    23461 ttactgataa gtttcggagc ttattaacag tcagtctcaa gggaggagtc atcctccctt
    23521 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg
    23581 catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata
    23641 gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga
    23701 aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg
    23761 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    23821 tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac
    23881 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    23941 atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga
    24001 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    24061 ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt
    24121 ccgatcataa aaaccaccgc ccattcccag gcgctgaccg tactcatcaa aggcgaccag
    24181 cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg
    24241 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    24301 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    24361 gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac
    24421 cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    24481 ttccagctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc
    24541 ttgtcgggat aatgtcagtg ggagctctgg tagttgcgtc atggctggtg ccagataaga
    24601 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    24661 gtgtcgtcgc agttttaagg cttctcggac gaaccgagca tgctcaccaa ccgcggagcg
    24721 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    24781 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    24841 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    24901 agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtctttgc tttttcttgc
    24961 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    25021 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    25081 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    25141 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    25201 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    25261 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    25321 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    25381 acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga tgatatgtgg
    25441 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    25501 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    25561 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    25621 cgatcgggct gatgcactgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    25681 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    25741 catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    25801 agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    25861 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    25921 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    25981 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    26041 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    26101 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    26161 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg
    26221 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac
    26281 tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg
    26341 ctgatgaaat cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca
    26401 ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat
    26461 cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacatacac
    26521 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc
    26581 acgaatttaa ccgccacggt gcgcgctatc cttcctataa caccattgta ggcagcggtg
    26641 aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac ggtgacctgg
    26701 tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc
    26761 cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg ctggagtctc
    26821 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag
    26881 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt aatgttgatg
    26941 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    27001 gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    27061 ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac
    27121 aatatcgcgg tatcggcatt cgcattgaag acgacattgt gattaccgaa accggcaacg
    27181 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggctgctg
    27241 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    27301 ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag cgactgcgcc
    27361 agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg cgggtacctg
    27421 tcagcaactg gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac
    27481 taccgtgcat gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta
    27541 ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc
    27601 attgctgcga aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc
    27661 ccgtactcag agtcatgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt
    27721 gctgatagcc gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca
    27781 gcaggagcct tacgaacaac tggcagtgat tgccaacgtt gctacttctg ttgcgcacga
    27841 aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    27901 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    27961 gagcgacgag aagttttgcc gtgaactcca gtctgccttt ggctggcgac tggggcaaat
    28021 tacccacgcc ggtaaacgca gtgcttatcc gctggcatta acccgcgccg ccagaccgat
    28081 tacccatcgc actgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca
    28141 agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca
    28201 tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca
    28261 gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg
    28321 ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc
    28381 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    28441 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    28501 cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac
    28561 cggaacctct ggcggtagat gcactaccac aactgcgcgt ttcggctatc aatgccgcca
    28621 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctcgctcgt agggccagtt
    28681 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatctcg tttgacgatc
    28741 aaagcatggg ctatagccat cttgggcaca tcgttgaaaa ctcagtgatt cactacgcgc
    28801 tgtggaacaa agcgcagcag tcgtcagata ttactctgtt ggcccccaca gaattacagc
    28861 aggtcgcctg gggagaaaat gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    28921 cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc
    28981 cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac
    29041 cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc
    29101 ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa gcgcagcgga
    29161 tgcagcaggc aagtgaagac gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    29221 ttggcttatg caaggttgag agcgcgcgtc aggtgttccc gctgacgggg cgttatgcgc
    29281 gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    29341 tggcggggca gggcgttaat ctcggattta tggacgccgc agagctgatt gctgaactga
    29401 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgc cgctatgaac
    29461 gtagccgcaa gcacagcgcg gcgctgatgc tggctggtat gcagggtttt cgcgatctct
    29521 tttccggcgc taatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca
    29581 cgcttcctgg cgttaagccg caacttatcc gccaggcaat gggattaaac gatttgcctg
    29641 aatggctgcg ttaaaaattt ctcctctgtt gtttatttga tacccatcac actttcatct
    29701 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    29761 cgcattatat tttctaatac catgattttt tgatttagtg ttttttgaca tttttttagc
    29821 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaattgg
    29881 tgtatttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    29941 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    30001 aacgattgcg ttggggaccg ggagtggctc cgatgctggg ttttgtggtg ataatttcac
    30061 catgaaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    30121 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    30181 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    30241 ttgatgtgtc gcatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    30301 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag aggcaaagcc ctttactcgg
    30361 ggatgttgaa tgcctctggc ggtgtgatag atgatctcat cgtctactac tttactgaag
    30421 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    30481 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    30541 tgcaagggcc gaatgctcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg
    30601 cggtggaagg gatgaaaccg ttctttggcg tgcaggtggg cgatctgttt attgccacca
    30661 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    30721 tctggcgtgc gctggtggaa gcgggcgtta agccctgcgg tctgggggcg cgtgacacgc
    30781 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    30841 tagccgccaa catgggctgg actatcgcct gggaaccgac agatcgtgac tttatcggtc
    30901 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    30961 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    31021 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    31081 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    31141 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    31201 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    31261 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    31321 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    31381 gggcgatgac tgcgcggttg ccgaatcggt aaaagcggcg tcagacattt atgcgccagt
    31441 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    31501 cgaaccgtat gcaggcggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    31561 atcactgctg gatgcgaccg catacgaagc attgttagaa gatgagtaac ggctttattc
    31621 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    31681 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    31741 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    31801 aacagcaaga aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga
    31861 ttgtgccgaa agatattcag cttgcgacac caccgcaggt tggcgcaccg gcgaccgaat
    31921 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    31981 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    32041 atccgggctg gtataccgcg tacactccgt atcagcctga agtctcccag ggccgccttg
    32101 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    32161 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    32221 gcaaactgaa aaatgccaac cgcttcttcg tggcttccaa tgtgcatccg caaacgctgg
    32281 atgtggtccg tactcgtgct gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    32341 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    32401 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    32461 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    32521 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    32581 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    32641 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    32701 ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    32761 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    32821 accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg
    32881 cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg
    32941 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg atcacccttg
    33001 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag tgtgctgctg ggcgataacc
    33061 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc
    33121 ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc tatcacagcg
    33181 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    33241 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    33301 tcacttggac ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    33361 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    33421 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    33481 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    33541 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    33601 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    33661 acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc
    33721 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    33781 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    33841 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    33901 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    33961 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    34021 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    34081 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    34141 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    34201 aaaccggcat cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc
    34261 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    34321 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    34381 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    34441 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    34501 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    34561 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgagtattt
    34621 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    34681 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    34741 atttaccctc accccgaccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg
    34801 aagattgagc cagtttgtta gcaatctcaa agatacgtca acgaattaat ttttctcgga
    34861 aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgt
    34921 attactgttg gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc
    34981 ggcgcaggaa gtgatgaact taataatgca agccggtggc aaagcattcg tgctccaggc
    35041 ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga
    35101 accgctagca gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa
    35161 ccttaccgca gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg
    35221 ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt
    35281 caatgtctct tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc
    35341 atcgaaaggg gcgattgatt cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg
    35401 gatccgcgtt aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcgcggc
    35461 gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    35521 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    35581 agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    35641 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    35701 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    35761 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    35821 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    35881 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    35941 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    36001 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    36061 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    36121 ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc aagataatcg
    36181 caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt aaatccgcga
    36241 cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac atcggtaaag
    36301 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acatgagtag
    36361 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    36421 gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    36481 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    36541 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    36601 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa tttacggttg
    36661 gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga gagggttatc
    36721 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    36781 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    36841 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    36901 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    36961 cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    37021 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    37081 ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta caggaaatag
    37141 taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat tctggctggg
    37201 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
    37261 cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
    37321 accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat gaccctgcct
    37381 gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta tatgattgaa
    37441 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
    37501 atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
    37561 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg agtaagttat
    37621 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
    37681 cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
    37741 ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg gcggcagtat
    37801 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
    37861 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
    37921 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
    37981 cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca aaattttatc
    38041 tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
    38101 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
    38161 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
    38221 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatg
    38281 ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac
    38341 cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt tgtgataaag
    38401 gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata aatgcctgat
    38461 gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg
    38521 catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg acgacctgta
    38581 ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta cgacgccagt
    38641 tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg acactacctg
    38701 ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc accatctctt
    38761 gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga ttggtcgcct
    38821 gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct tcaatgttta
    38881 atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca
    38941 tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt ttaaaccata
    39001 gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt tcgctaaaga
    39061 gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt
    39121 ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc agctgcggtt
    39181 cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac agacgtaaat
    39241 tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga tcttctgcca
    39301 tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc gcgccagtaa
    39361 tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg
    39421 taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt gcttaacggc
    39481 gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca ctacgcgatc
    39541 tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg tgaattaatt
    39601 tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa
    39661 tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa caaagcccga
    39721 attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat gccgtttttc
    39781 gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg tctgctggaa
    39841 tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc cgatgcagaa
    39901 ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc tgtggcaatc
    39961 tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg ggctggttgc
    40021 cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga tggtgttact
    40081 ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc gccaggggga
    40141 aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa
    40201 agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg gcggtaaacg
    40261 gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg atttacggcg
    40321 gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat gcgctaagcc
    40381 gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg caccagctct
    40441 gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga cccacgaaca
    40501 gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc gttggccagt
    40561 agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg agcgcatcga
    40621 ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata attttacctt
    40681 caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg atttcaccaa
    40741 aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat ttcggggagt
    40801 cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg attttttctg
    40861 ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc ataacgtaag
    40921 cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta gtcacggcac
    40981 aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac aggagctggc
    41041 acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg aaaatacgtt
    41101 gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac
    41161 gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc tggagggcgg
    41221 gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt tccagtatct
    41281 ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc
    41341 ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac aggcgccatt
    41401 aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt atgctaccgg
    41461 gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc gtcagggcgt
    41521 agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt
    41581 ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat
    41641 tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct tctggcaccg
    41701 cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt
    41761 gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg tggtgcagat
    41821 gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg ctaccgagcg
    41881 tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag
    41941 atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag gctttgctca
    42001 ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat
    42061 tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg tgttgtacat
    42121 caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg gcacggctcc
    42181 ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat
    42241 cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata ttacctgtgg
    42301 ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga tcaccgtgcg
    42361 ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa tgaaagctat
    42421 ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc
    42481 accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc agcttggcgt
    42541 tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt accagccgcc
    42601 gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta aataattcgc
    42661 gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct
    42721 gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa
    42781 ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc
    42841 gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc
    42901 gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc
    42961 agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa
    43021 gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt
    43081 tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat
    43141 caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc
    43201 gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg
    43261 gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat cgcaattcct
    43321 aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt cgtgccgctg
    43381 gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc cggaaagtgc
    43441 cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc
    43501 agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg
    43561 tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat
    43621 gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc
    43681 ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg
    43741 tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt
    43801 tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt
    43861 cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac actctacccc
    43921 ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat
    43981 aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct ggccccttcg
    44041 ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat gaccatggaa
    44101 gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt
    44161 gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg
    44221 gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt cgataccgct
    44281 ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga tatcaacgag
    44341 tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat ttagcgtcat
    44401 cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc gcattttgac
    44461 tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa tcgcattcag
    44521 gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg ccaagaaaga
    44581 gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg aacccgaacg
    44641 cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca
    44701 aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag aagctgacga
    44761 cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa aactggcgca
    44821 gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt acctcgatat
    44881 tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg agcgtatgta
    44941 tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt cggaaggtga
    45001 agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt acggctggct
    45061 gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg
    45121 tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg atattgatat
    45181 cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca
    45241 gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg ggatcgtgac
    45301 ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga agcagatgaa
    45361 agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga
    45421 taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg atgactcccg
    45481 cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc tggacggcag
    45541 cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca acatgtctga
    45601 acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga aaacgcgtcg
    45661 tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga
    45721 tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag aactggaagc
    45781 gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta tgggtaaagc
    45841 gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga
    45901 tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg acatcctcgg
    45961 cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact gtaccgagct
    46021 gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct tgcaggatca
    46081 ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat cccgtaacac
    46141 ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga accgcggctt
    46201 tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt
    46261 tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc cggaactgta
    46321 cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg
    46381 taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac tctacatggc
    46441 ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta ctctggcaca
    46501 ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact tcggtaaacc
    46561 gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa ccgacatggc
    46621 ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta tccacgttga
    46681 gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg cagaagcaca
    46741 tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc tggcgcgtcg
    46801 taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat
    46861 cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct tcctggatca
    46921 ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag attacgttac
    46981 cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg accgtatggt
    47041 aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc
    47101 ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa
    47161 gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc
    47221 acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct aaatcacacc
    47281 attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa
    47341 ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg tagcgaaagt
    47401 caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg caacggcgga
    47461 tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag ttagtccaca
    47521 cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc cccttggcat
    47581 taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt gcggcatact
    47641 tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt tccgtttgca
    47701 taatttctca catgtaattc tgatcactca tcattataga taattgaaga gtgaaatgat
    47761 taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac
    47821 catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg caacacagtc
    47881 agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag cgcgcattaa
    47941 aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga agccacatac
    48001 gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg tcagcatgat
    48061 gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg agcataatgc
    48121 cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg tgagagagcg
    48181 tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa cggttggtgg
    48241 tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg aacatcacta
    48301 gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc atttttggca
    48361 ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg ctatgaacgc
    48421 gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag ctgttaaacg
    48481 tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata tcgtgtgaag
    48541 agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac tcgataaaca
    48601 cgatgat
//
LOCUS       DAEBXD010000021        48312 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEBXD010000021 DAEBXD010000000
VERSION     DAEBXD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48312
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.021"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            187..480
                     /locus_tag="KJL94_001195"
     CDS             187..480
                     /locus_tag="KJL94_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD4409624.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KJL94_001196"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KJL94_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBD4409625.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="KJL94_001197"
     CDS             complement(1922..2374)
                     /locus_tag="KJL94_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBD4409626.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KJL94_001198"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KJL94_001198"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBD4409627.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="KJL94_001199"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="KJL94_001199"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBD4409628.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISDPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KJL94_001200"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KJL94_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBD4409629.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KJL94_001201"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KJL94_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBD4409630.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KJL94_001202"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KJL94_001202"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBD4409631.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KJL94_001203"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KJL94_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBD4409632.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KJL94_001204"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KJL94_001204"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD4409633.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KJL94_001205"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KJL94_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBD4409634.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KJL94_001206"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KJL94_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBD4409635.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="KJL94_001207"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="KJL94_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBD4409636.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="KJL94_001208"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="KJL94_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBD4409637.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="KJL94_001209"
     CDS             complement(15085..15582)
                     /locus_tag="KJL94_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBD4409638.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="KJL94_001210"
     CDS             complement(15579..15941)
                     /locus_tag="KJL94_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD4409639.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="KJL94_001211"
     CDS             complement(15931..16278)
                     /locus_tag="KJL94_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD4409640.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="KJL94_001212"
     CDS             16387..16800
                     /locus_tag="KJL94_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409641.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="KJL94_001213"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="KJL94_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="KJL94_001214"
     CDS             complement(18324..20039)
                     /locus_tag="KJL94_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBD4409642.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="KJL94_001215"
     CDS             20206..21099
                     /locus_tag="KJL94_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBD4409643.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="KJL94_001216"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="KJL94_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23527)
                     /locus_tag="KJL94_001217"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="KJL94_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="KJL94_001218"
     CDS             23822..24496
                     /locus_tag="KJL94_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD4409644.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="KJL94_001219"
     CDS             complement(24535..26196)
                     /locus_tag="KJL94_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBD4409645.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KJL94_001220"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KJL94_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBD4409646.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KJL94_001221"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KJL94_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBD4409647.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="KJL94_001222"
     CDS             27650..27982
                     /locus_tag="KJL94_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBD4409648.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29203)
                     /locus_tag="KJL94_001223"
                     /pseudo
     CDS             complement(27984..29203)
                     /locus_tag="KJL94_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            29304..30404
                     /gene="cbrA"
                     /locus_tag="KJL94_001224"
     CDS             29304..30404
                     /gene="cbrA"
                     /locus_tag="KJL94_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBD4409649.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30365..31702)
                     /locus_tag="KJL94_001225"
     CDS             complement(30365..31702)
                     /locus_tag="KJL94_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4409650.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31777..32925)
                     /gene="dgoD"
                     /locus_tag="KJL94_001226"
                     /pseudo
     CDS             complement(31777..32925)
                     /gene="dgoD"
                     /locus_tag="KJL94_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32922..33539)
                     /gene="dgoA"
                     /locus_tag="KJL94_001227"
     CDS             complement(32922..33539)
                     /gene="dgoA"
                     /locus_tag="KJL94_001227"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBD4409651.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33523..34401)
                     /gene="dgoK"
                     /locus_tag="KJL94_001228"
     CDS             complement(33523..34401)
                     /gene="dgoK"
                     /locus_tag="KJL94_001228"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBD4409652.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34398..35087)
                     /gene="dgoR"
                     /locus_tag="KJL94_001229"
     CDS             complement(34398..35087)
                     /gene="dgoR"
                     /locus_tag="KJL94_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBD4409653.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35365..36021
                     /locus_tag="KJL94_001230"
     CDS             35365..36021
                     /locus_tag="KJL94_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409654.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36067..36879)
                     /gene="yidA"
                     /locus_tag="KJL94_001231"
     CDS             complement(36067..36879)
                     /gene="yidA"
                     /locus_tag="KJL94_001231"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBD4409655.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36993..37391)
                     /locus_tag="KJL94_001232"
     CDS             complement(36993..37391)
                     /locus_tag="KJL94_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBD4409656.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37631..40045)
                     /gene="gyrB"
                     /locus_tag="KJL94_001233"
     CDS             complement(37631..40045)
                     /gene="gyrB"
                     /locus_tag="KJL94_001233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBD4409657.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40074..41147)
                     /gene="recF"
                     /locus_tag="KJL94_001234"
     CDS             complement(40074..41147)
                     /gene="recF"
                     /locus_tag="KJL94_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBD4409658.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41147..42247)
                     /gene="dnaN"
                     /locus_tag="KJL94_001235"
     CDS             complement(41147..42247)
                     /gene="dnaN"
                     /locus_tag="KJL94_001235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBD4409659.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42252..43655)
                     /gene="dnaA"
                     /locus_tag="KJL94_001236"
     CDS             complement(42252..43655)
                     /gene="dnaA"
                     /locus_tag="KJL94_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBD4409660.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43952..44032
                     /locus_tag="KJL94_001237"
     CDS             43952..44032
                     /locus_tag="KJL94_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409661.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44262..44402
                     /gene="rpmH"
                     /locus_tag="KJL94_001238"
     CDS             44262..44402
                     /gene="rpmH"
                     /locus_tag="KJL94_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBD4409662.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44419..44778
                     /gene="rnpA"
                     /locus_tag="KJL94_001239"
     CDS             44419..44778
                     /gene="rnpA"
                     /locus_tag="KJL94_001239"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBD4409663.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44742..44999
                     /gene="yidD"
                     /locus_tag="KJL94_001240"
     CDS             44742..44999
                     /gene="yidD"
                     /locus_tag="KJL94_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBD4409664.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45002..46648
                     /gene="yidC"
                     /locus_tag="KJL94_001241"
     CDS             45002..46648
                     /gene="yidC"
                     /locus_tag="KJL94_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBD4409665.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46754..48118
                     /gene="mnmE"
                     /locus_tag="KJL94_001242"
     CDS             46754..48118
                     /gene="mnmE"
                     /locus_tag="KJL94_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBD4409666.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11993 a        13059 c        12205 g        11055 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccgacccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg tcctgataca
    29221 aaacggttcc cgccatcata cttcacaagg acttcacttt cagacaatag tcttttgcca
    29281 tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt ggcctcggcc
    29341 cggcagggtc ggcgttggca cgaaagttag ccggcaaaat gcaggtgatc gcgctggata
    29401 aaaagcacca gtgcggtact gaaggtttca gcaaaccttg cggcggtctg ctggcaccgg
    29461 acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtg atcgccaatc
    29521 cacagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    29581 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    29641 ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag gatgataaat
    29701 ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc tatctggtcg
    29761 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    29821 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    29881 tctttgataa tgcgataact gactgttact catggagtat cagcaaagat ggttatttta
    29941 tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    30001 aaatgagcgc ctttcagttc cagtttggca aagcggtgaa aagtgagaaa tgcacggtgt
    30061 tgtttccctc acgctggcag gattttgtct gtggtaagga caacgccttt ctgattggtg
    30121 aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg ctggatagcg
    30181 cagagattct gcgtgcggtg ttcctgaaac agccggagaa gagcaacgcc gcttactggc
    30241 gcgccacaca caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    30301 ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt ccacagttga
    30361 aagattatcc aacgcgcttc acatcaccca ccagcaggat gtaagagagc gcgccaatca
    30421 acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc gccaggtacc
    30481 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccgccgg
    30541 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    30601 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    30661 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    30721 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    30781 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    30841 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgcagaag
    30901 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    30961 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    31021 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    31081 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    31141 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    31201 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggga
    31261 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    31321 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccttggaat aaagtcacca
    31381 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    31441 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    31501 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaagttag
    31561 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    31621 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    31681 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    31741 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    31801 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    31861 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    31921 catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    31981 aatgcccata ctttgttact gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    32041 agccgccagt gcaatcggtc cgaacggaca gtgcggcgca agggtcacat cataggcttc
    32101 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    32161 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    32221 ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    32281 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    32341 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    32401 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    32461 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    32521 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    32581 tttaattttg tcgcgaacca ggccgcccat cagttgccag actggcgcat tcagcacttt
    32641 gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca ggattggacc
    32701 gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc gcgaaggatc
    32761 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    32821 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    32881 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    32941 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga
    33001 taaagatcgc tgcctaagcc cgccccaaca caacctgcgt ctatccactg cgccaggttt
    33061 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac cgcttttaac
    33121 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    33181 gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac ggtcatgccg
    33241 tagcccaccg cgcggcggat cacttcacta tggatattgg gcgtaacgat gagctgacag
    33301 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    33361 gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg tggggaattc
    33421 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    33481 tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg ccactgcatg
    33541 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg ccacgtcgca
    33601 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    33661 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    33721 accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat gcgaggcgcg
    33781 aacttcaaaa agctgcggca atatggcggg cgcattaagg ccgcgctcaa ggccagccgc
    33841 gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaatcaatg agtgatttaa
    33901 cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttgattt gctggctatc
    33961 ggcctgcacc catttgcaat gcgtaccggg cataacataa agagaggaag gagccagagc
    34021 tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat cgtcatgaga
    34081 gacgcataat ccgggaataa tccagatatt gtcgccaaca gacgttaatt gttcgccaat
    34141 agacgaaaaa cgggcaggaa cagataaata cggagcaact ttccagccga cattgctgcc
    34201 gaccattcct gccatcacca ctggcgtatt ttcttcacgc cagtcggtcg tgacttctgc
    34261 taacaccaca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg attgcctgct
    34321 ctccaggcag tggtcgccct gataaagcca ggcgcgcaga ttggtcgatc cccagtcaat
    34381 tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct ggcaatcatg
    34441 gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc gtatcgcatc gaacagcgcc
    34501 ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt tcgttcaaaa
    34561 accgcccgct gcagcgaact gatcgcaatg ctaagttgct gtaacaccgg gttatgcacc
    34621 gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc gcggtcctga
    34681 ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct ggaggtcgcg
    34741 cgctctgctg cccaacgggc aatcgccggt tccaccagat ttcgcacttc gctcatggca
    34801 ctgataagcc gtgggtcgta gtcgttttcc agcacccatt gcagtacgtc agtgtcgagg
    34861 taattccact ggttacgcgg tgccacaaac gccccgcgat aacgtttcat ttcaatcagc
    34921 cgcttcgcca tcagcgaacg gaacacctca cggatgatgt tgcgcgaggt tgcaaactct
    34981 tcacagagct ccgcctcagc cggaagtggc gagccaggta cgtatttgcc gtgaacgatc
    35041 tgtttaccca gcgtaatgac aatgcgatcg gttttattga gagtcatgga gagtccttgt
    35101 gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa ctttgtagta
    35161 ccagcgtgat gacgttcgcg tttgccgcgc gcgtaatgta gtacaaactt atattgttat
    35221 gctacaattt agatcaaaaa aagaacaata cataaaaaaa tgacatgcgt cgggcagaaa
    35281 tctgaaaagg gatatcaggc gctaaacagg agggaaagaa gagtatgctt tcaacggctt
    35341 agctactcgt ttaaaggatt aatcatgaag ttgaatttta agggattttt taaggctgcc
    35401 ggtttattcc cactggcgct gatgctttca ggctgtatct cgtatgctct ggtttcccat
    35461 accgcaaagg gtagttcagg aaagtatcaa tcgcagtcag acaccatcac tgggctatcg
    35521 caggcaaaag atagtaatgg aacaaaaggc tatgtttttg taggggaatc gctggattac
    35581 cttatcactg atggtgccga tgacatcgtt aagatgctca atgatccagc acttaaccgg
    35641 cacaatattc aggttgccga tgacgcaaga tttgttttaa atgcggggaa aaagaaattt
    35701 accggcacaa tatcgcttta ctactactgg aataacgaag aagaaaaggc actggcaacg
    35761 cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt cactggaaaa cctaaaaggc
    35821 acaatccatg agaaaaataa aaacatggat tactcaaagg tgatggcgtt ctaccatcca
    35881 tttaaagtgc gattttatga atactattca cccagaggca ttccggatgg tgtttccgca
    35941 gcattactgc cagtgactgt tacactggac atcattactg caccgctgca atttctggtt
    36001 gtatatgcag taaaccaata atcagtaagc gggcaaacgc gtttatgctg tttgcccgct
    36061 tactgattaa ttcagcacat acttctcaat agcaaacgcc acgccatctt caaggttgga
    36121 ttttgtgaca aagttcgcca cttctttcac cgaaggaata gcgttatcca tcgccacacc
    36181 gacgcctgca tattcaatca ttgcgatgtc gttttcctga tcgccaatcg ccatgatttc
    36241 ttccggttta atacctaaca cgtcggccag tgatttcaca cccgtacctt tgttaacgcg
    36301 tttatcgagg atttcgagga agtacggcgc acttttcagc acggtatatt tctctttcac
    36361 ttcctgcgga atacgcgcga tagcctggtc gaggatggcg ggttcatcaa tcatcatcac
    36421 tttcaggaac tgggtattgg ggtccatttt ctccgcttcg cagaacacca gcggaattgt
    36481 ggcaacgaag gattcatgca ccgtgtagta gctgatatcg cggttagcgg tgtacagcgt
    36541 ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga gagagttttt ccaggaaacg
    36601 atagtcgtca tagctgagag cggtttgcgc cacggtgcta ccatcagcgg ccttctgtac
    36661 cagcgcgccg ttataagtaa tgcagtagtc gcccggctgt tccatatgca gctctttcag
    36721 gtagttgtgc acacctgcat acggacgacc cgtcgttagc acgacattca cgccacgggc
    36781 gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg gtgtgatcgg gcagcagaag
    36841 ggtgccatcc atatcgatag caatgagttt gatagccatg agttcccaag gtagattagt
    36901 tcctgaccca tgctaacgcg attccgctca aaaatcagta caacacccga gggaaaaggg
    36961 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    37021 tccatgcctg cggaaagcag atcgttattg gcttgtgctg acacttcacc ttgcggtgag
    37081 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagccgt actggtatcc
    37141 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ctccagttgc
    37201 tcgccgctaa ctgattgatt tctctgttga ttactcaacc aggttgagag aatggcccct
    37261 aagccgccac tttgcagttt ttccagcagc acctgaatgc caccctgctc ctcaacccaa
    37321 cttaaaatcg cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    37381 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatctg
    37441 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    37501 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgcaaaat
    37561 caatatattg caggagtctc gtaggactaa taagcgtagc gcatcaggca cgctcgcatg
    37621 gttaacgcca ttaaatatca atattcgccg ctttcagggc gttctcttca ataaacgcac
    37681 gacgcggttc tacggcgtcg cccatcagcg tggtgaacaa ctggtcagcg gcaatcgcat
    37741 ccttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    37801 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggcca cgacgggact
    37861 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    37921 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    37981 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    38041 gggtacgcac gcgaacaatc ggctcgaaca ggttttgttc ggcattggtg tggacatcaa
    38101 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    38161 agcgggtaac ggtctgctca tcagaaaggt cagcttccgt caacgtcggc tgatagataa
    38221 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    38281 cgttgtactc agataccagt ttctctaacg cttcgccagc caatgccggt gcactggcgt
    38341 tggtgtgcag cgttgcgccg tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    38401 cgtctttaat gtactgttcc tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    38461 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaagaag gtcaacagca
    38521 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    38581 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    38641 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcgacg ttaaggattt
    38701 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    38761 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    38821 ctgccagttt gcccggcagg ccagctaagt cgagcgcacc tttacggcgg gtcatttcac
    38881 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    38941 tcgcatcagt tgggttttcc agcaggtatt ccgccagcag ttcgttcatc tgctgttcaa
    39001 ccgccgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    39061 ccggcacttt tacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    39121 tgactttggc ttttttgctg tagccttctt tgtccatgta ggcattcagg gtacgggtca
    39181 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    39241 agcagtagat gttttcctgg aagccatcgt tccactgcag cgccacttca acgccaatac
    39301 cgtctttttc ggtggagaag tagaagatat ttgggtggat cggcgttttg ttcttgttca
    39361 gatattcaac gaacgctttg atgccgcctt catagtggaa gtggtcttct ttgccgtcac
    39421 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    39481 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    39541 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccagcgga gcctgcggta
    39601 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctgaataacc agctccagtt
    39661 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    39721 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    39781 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    39841 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    39901 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    39961 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    40021 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatct
    40081 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    40141 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    40201 agcaacccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    40261 cgccgcccgc tttcacgagt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    40321 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    40381 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    40441 cgttccagca cctcagcgta ctctgtctct ttctcccagc cgcgctggaa agagaaagtc
    40501 agagaaaact cagggagaaa ttgcttacag gtatcatcca tatcggccgc gataccggcg
    40561 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagttc tttatcccac
    40621 gggcgtagct gttcgtaacg agtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    40681 cgcttgagat tgctccaggc ggtgaaaaat ccaggttcgt tgtgaaagca tccccagtcg
    40741 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    40801 aactgcatcg gcatcaggtg cgccagttcc gcgactttat gcccgtccgt accgtcaata
    40861 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgccgtctc gcgctcttcg
    40921 ccctgtaatc gcccgtggag aacaaatgcc tcctgctcat ggcgaatgac gcgaccaatc
    40981 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    41041 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    41101 gtttcaatat tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    41161 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    41221 aatcggtcag catcatgcgg acgttttcgc atttcagcgc gttcagaaca tccagcacat
    41281 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    41341 ccgcttcttc ctgttccggg ttgttggcag taattttcag ctggttttcg ctgacataca
    41401 ggcgcacacc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    41461 gcagatcgca gcctgcttcc agatgtttgt ccgggttctt cggcagaacg cgacggtaat
    41521 ccgggaagcg accatccacc agtttcgagg tgaaaataaa gtcgccaacg tgggcacgaa
    41581 tgttattgct gccaatctgc acacgcagca gattgtcgcc gccgtcgagc atacgcatca
    41641 gttcaatcac gcctttacgc ggtacgatca ccgaatggct tggcaaagat tgaccaattg
    41701 gcattgaaca gaccgccaga cggtggccgt cggttgccac ggtgcgcagt tcttcacctt
    41761 cggtttcaaa cagcataccg tttaagtaat agcgaacatc ctgatgcgcc atcgaaaact
    41821 gggttgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    41881 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    41941 gcccggagcg caccagcatc cgttcacctt ccagctgcac ggcaatttcc gcgccttcag
    42001 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    42061 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    42121 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    42181 cacctaacgg accgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    42241 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    42301 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    42361 cgcccaccaa acgcatcgcc aatctctggc agactgtggt tagtcagctc tttcgctagc
    42421 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    42481 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    42541 ttttcctgca atgccagcaa gtcgcgcagc gcctcacgca cgaagtcgat ggtgatcgcc
    42601 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    42661 ttagatcgta gacgcttggc gataaagaac gccacttcac ccggcaaacg aatgtcattt
    42721 tcgtcggcct ttttcatcag gatcgccaca cgggtttcca gctctggcgg ttcgatcgcc
    42781 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    42841 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    42901 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    42961 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    43021 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    43081 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    43141 ccataaagga acaacgggtt ataggcaccg ccagggttat ccgccacctg gcaagccgcc
    43201 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    43261 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    43321 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    43381 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    43441 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    43501 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    43561 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    43621 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    43681 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    43741 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    43801 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    43861 ctatttatcc acaggacttt ccagaactcg ctaagtgtaa acgatcctgc cgcgaggcgg
    43921 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    43981 atctaataaa gatccaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    44041 ccggcgcgcc atgctggttt ccactggtgt gaggtcgtac attttccctg cgaaaaggtg
    44101 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg tgtgtacaat tattacaatc
    44161 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    44221 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    44281 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    44341 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    44401 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    44461 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    44521 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    44581 aacgttcgac gcgcccatga acgcaatcgg attaaacgcc tgacgcgtga aagcttccgt
    44641 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    44701 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    44761 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    44821 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    44881 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    44941 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    45001 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    45061 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    45121 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    45181 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    45241 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgtt
    45301 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    45361 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    45421 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    45481 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    45541 gcagaacgct ggcgagaaac cgctggaaat ctcctcgttt ggtcagttga agcaatccat
    45601 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgtgg
    45661 cgcggcgtac tccacgcctg acgagaagta tgagaaatac aagttcgata ccattgccga
    45721 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcgatgctgc aacagtattt
    45781 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    45841 taacggcatc gtcgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    45901 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    45961 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    46021 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    46081 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    46141 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    46201 caaacagcgt atcagtcagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    46261 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    46321 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    46381 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    46441 gatgtcgccg accactgtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    46501 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtactgtact atatcgtcag
    46561 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    46621 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    46681 agttgaccgc cttttttctt ttcgtagggc ggataagcac cgcgcatccg ccacacaaag
    46741 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    46801 tggcggcgtt ggcatcctgc gcatctccgg cttcaaagcc cgtgaagttg ccgaaaccgt
    46861 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    46921 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    46981 agatgtgctg gaactgcaag gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    47041 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    47101 tcttaacgat aaacttgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    47161 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    47221 taatcatctg gtagaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    47281 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    47341 gatggccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    47401 gatgaaagta gtgattgccg gacgtcctaa tgccggtaaa tcgagcctgt taaacgcgct
    47461 ggcgggccgt gaagcggcaa tcgtaaccga tatcgccgga accacgcgtg acgtgctgcg
    47521 tgagcatatc cacattgacg gaatgccgct gcatatcatc gacactgccg ggctacgtga
    47581 ggccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    47641 cgaccgcgtg ctgtttatgg tcgatggcac cacgacagac gccgttgatc cggcagagat
    47701 ctggccggaa tttatcgccc gtctgcccgc gaaactgccg atcaccgtgg tacgcaataa
    47761 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    47821 tctctcggca aggactggtg aaggcgtgga cgtgctgcgt aaccatctca aacagagcat
    47881 gggctttgac accaatatgg aaggcggctt cctggcgcgc cgtcgccact tacaggcgct
    47941 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttgggag cctgggcagg
    48001 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    48061 atttacttca gacgacctgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    48121 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    48181 gttatgcctc aacccttttt taaacattaa aattcttacg taatttataa tctttaaaaa
    48241 aagcatttaa tactgctccc cgaacgattg tgattcgatt cacatttaaa caatttcaga
    48301 atagacaaaa at
//
LOCUS       DAEBXD010000022        47308 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEBXD010000022 DAEBXD010000000
VERSION     DAEBXD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47308
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.022"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="KJL94_001243"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="KJL94_001243"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBD4409667.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KJL94_001244"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KJL94_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBD4409668.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KJL94_001245"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KJL94_001245"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBD4409669.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KJL94_001246"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KJL94_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBD4409670.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KJL94_001247"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KJL94_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBD4409671.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KJL94_001248"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KJL94_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBD4409672.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KJL94_001249"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KJL94_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBD4409673.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KJL94_001250"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KJL94_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBD4409674.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KJL94_001251"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KJL94_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBD4409675.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KJL94_001252"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KJL94_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBD4409676.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KJL94_001253"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KJL94_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBD4409677.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KJL94_001254"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KJL94_001254"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBD4409678.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPLECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="KJL94_001255"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="KJL94_001255"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBD4409679.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="KJL94_001256"
     CDS             11233..12087
                     /locus_tag="KJL94_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4409680.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="KJL94_001257"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="KJL94_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBD4409681.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="KJL94_001258"
     CDS             complement(13550..14689)
                     /locus_tag="KJL94_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBD4409682.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KJL94_001259"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KJL94_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBD4409683.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="KJL94_001260"
     CDS             15875..17236
                     /locus_tag="KJL94_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBD4409684.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="KJL94_001261"
     CDS             17233..17751
                     /locus_tag="KJL94_001261"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBD4409685.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="KJL94_001262"
     CDS             17751..18071
                     /locus_tag="KJL94_001262"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBD4409686.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="KJL94_001263"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="KJL94_001263"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBD4409687.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="KJL94_001264"
     CDS             18890..20092
                     /locus_tag="KJL94_001264"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBD4409688.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="KJL94_001265"
     CDS             20189..20611
                     /locus_tag="KJL94_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD4409689.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="KJL94_001266"
     CDS             complement(20659..21531)
                     /locus_tag="KJL94_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBD4409690.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTTLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="KJL94_001267"
     CDS             complement(21543..22637)
                     /locus_tag="KJL94_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4409691.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="KJL94_001268"
     CDS             complement(22670..23668)
                     /locus_tag="KJL94_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4409692.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="KJL94_001269"
     CDS             complement(23693..25204)
                     /locus_tag="KJL94_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD4409693.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVTNGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="KJL94_001270"
     CDS             complement(25227..26210)
                     /locus_tag="KJL94_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBD4409694.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="KJL94_001271"
     CDS             complement(26307..29408)
                     /locus_tag="KJL94_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBD4409695.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="KJL94_001272"
     CDS             complement(29415..29588)
                     /locus_tag="KJL94_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409696.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="KJL94_001273"
     CDS             29706..30899
                     /locus_tag="KJL94_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBD4409697.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KJL94_001274"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KJL94_001274"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBD4409698.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="KJL94_001275"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="KJL94_001275"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBD4409699.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KJL94_001276"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KJL94_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBD4409700.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KJL94_001277"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KJL94_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBD4409701.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KJL94_001278"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KJL94_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBD4409702.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KJL94_001279"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KJL94_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBD4409703.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KJL94_001280"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KJL94_001280"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBD4409704.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="KJL94_001281"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="KJL94_001281"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBD4409705.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KJL94_001282"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KJL94_001282"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBD4409706.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KJL94_001283"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KJL94_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBD4409707.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="KJL94_001284"
     CDS             45620..46468
                     /locus_tag="KJL94_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBD4409708.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47308)
                     /locus_tag="KJL94_001285"
                     /pseudo
     CDS             complement(46807..47308)
                     /locus_tag="KJL94_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11282 a        12958 c        11893 g        11175 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgagcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg tcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tggtaacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg cattacgt
//
LOCUS       DAEBXD010000023        46762 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEBXD010000023 DAEBXD010000000
VERSION     DAEBXD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46762
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.023"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..1014
                     /locus_tag="KJL94_001286"
     CDS             <1..1014
                     /locus_tag="KJL94_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HBD4409709.1"
                     /translation="NEQATQELASTSTRLSGGMYADAHAEWGVKPKILGVGGGVGIRG
                     GFKGSLDATDDDSHSASSGSRATHDARHDIDAKASKDFKEASDYFTSRKVSESGSHTD
                     NNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQFAQ
                     YVMKRAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNAWRESRGDIGKGME
                     SVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRNDVKHQVDNMVTEYKGNIGDTQNSIH
                     GEENIVDRQYSELKNNHKQEEIQQNNRYNEENKRQILMPTPSEDALKQMVDDKKERLK
                     GPL"
     gene            1011..1520
                     /gene="traS"
                     /locus_tag="KJL94_001287"
     CDS             1011..1520
                     /gene="traS"
                     /locus_tag="KJL94_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBD4409710.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            1534..2265
                     /locus_tag="KJL94_001288"
     CDS             1534..2265
                     /locus_tag="KJL94_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBD4409711.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            2468..3205
                     /locus_tag="KJL94_001289"
     CDS             2468..3205
                     /locus_tag="KJL94_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409712.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            3256..5481
                     /gene="traD"
                     /locus_tag="KJL94_001290"
     CDS             3256..5481
                     /gene="traD"
                     /locus_tag="KJL94_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBD4409713.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGHQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEM
                     EQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDD
                     F"
     gene            5481..10751
                     /gene="traI"
                     /locus_tag="KJL94_001291"
     CDS             5481..10751
                     /gene="traI"
                     /locus_tag="KJL94_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBD4409714.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            10771..11517
                     /gene="traX"
                     /locus_tag="KJL94_001292"
     CDS             10771..11517
                     /gene="traX"
                     /locus_tag="KJL94_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBD4409715.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            11576..12436
                     /locus_tag="KJL94_001293"
     CDS             11576..12436
                     /locus_tag="KJL94_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4409716.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            12539..13099
                     /locus_tag="KJL94_001294"
     CDS             12539..13099
                     /locus_tag="KJL94_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBD4409717.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            13228..13440
                     /locus_tag="KJL94_001295"
     CDS             13228..13440
                     /locus_tag="KJL94_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBD4409718.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            13685..14146
                     /locus_tag="KJL94_001296"
     CDS             13685..14146
                     /locus_tag="KJL94_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD4409719.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTEMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            14192..14401
                     /gene="hha"
                     /locus_tag="KJL94_001297"
     CDS             14192..14401
                     /gene="hha"
                     /locus_tag="KJL94_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD4409720.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYGRIPKEIWKYVR"
     gene            14440..15030
                     /locus_tag="KJL94_001298"
     CDS             14440..15030
                     /locus_tag="KJL94_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBD4409721.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            15270..15518
                     /gene="repA"
                     /locus_tag="KJL94_001299"
     CDS             15270..15518
                     /gene="repA"
                     /locus_tag="KJL94_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBD4409722.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            15763..15837
                     /gene="tap"
                     /locus_tag="KJL94_001300"
     CDS             15763..15837
                     /gene="tap"
                     /locus_tag="KJL94_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBD4409723.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGRCD"
     gene            15830..16687
                     /gene="repA"
                     /locus_tag="KJL94_001301"
     CDS             15830..16687
                     /gene="repA"
                     /locus_tag="KJL94_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBD4409724.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            17389..17649
                     /locus_tag="KJL94_001302"
     CDS             17389..17649
                     /locus_tag="KJL94_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409725.1"
                     /translation="MAENGYGLAGQGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISICWWLCLKGYILYIKPDNTQIARQMSIMHPVIACL"
     gene            17756..18019
                     /locus_tag="KJL94_001303"
     CDS             17756..18019
                     /locus_tag="KJL94_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBD4409726.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSAQLRQQLQGRYQELRNEL"
     gene            18009..18308
                     /locus_tag="KJL94_001304"
     CDS             18009..18308
                     /locus_tag="KJL94_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD4409727.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLVENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFIL"
     gene            complement(18344..19009)
                     /locus_tag="KJL94_001305"
     CDS             complement(18344..19009)
                     /locus_tag="KJL94_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409728.1"
                     /translation="MMFWNRNKKDKATIANEKKRFNHLLSVAEKLPVKALPDLIRAIV
                     RPVQSDFLLAVAEEGTDARPDMTPQEFFFKGLTQIQSYEKMKECEMNGADYPLSLASD
                     IVLPWPWSLPRYIDNVSRIGTHKGKPWKQDKNNHYVDLWLPWRIGFVHGGNHSITAGI
                     LAGEGSLIPEHVYDMSWMFELVRTDGNNWFVEGQKAEAVKSGRSAAVFEIGRLLVEGA
                     KER"
     gene            complement(19579..20142)
                     /locus_tag="KJL94_001306"
     CDS             complement(19579..20142)
                     /locus_tag="KJL94_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD4409729.1"
                     /translation="MDIIEKRITKRHLSESELSGVNYYNCIFERIQLDNFNFRDCEFE
                     KCRFVNCSIKNLKLNFFKLIDCEFKDCLLQGVNAADIMFPCTFSLVNCDLRFVDFISL
                     RLQKSIFLSSRFRDCLFEETDLRKSDFTGSEFNNTEFRHSDLSHCDFSMTEGLDINPE
                     INRILSIKIPQEAGLKILKRMGVVVGG"
     gene            complement(20146..20877)
                     /locus_tag="KJL94_001307"
     CDS             complement(20146..20877)
                     /locus_tag="KJL94_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD4409730.1"
                     /translation="MTIPLLEINSLSFSYKVNLPPVFNNLSLKIEQGELIGLLGENGA
                     GKTTLFNLIRGGVSNYEGTLKRNFSGGELVSLPQVINLSGTLRNEEVLDLICCFNKLT
                     KKQAWTELNHKWNDNFFIRYDKIRRKRTYTVSYGEKRWLIISLMLTLCKNARLFLLDE
                     PTVGIDIQYRMMLWELINKITADGKTVFFSTHIFDELTRDKIPFYMLSKNSINRYSDM
                     SDFIQSNNETTPEKAFIKEVMGTGD"
     gene            complement(20874..21599)
                     /gene="mcbE"
                     /locus_tag="KJL94_001308"
     CDS             complement(20874..21599)
                     /gene="mcbE"
                     /locus_tag="KJL94_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17 immunity protein McbE"
                     /protein_id="HBD4409731.1"
                     /translation="MVTLKMAIIFLMEQIRTPFSLIWTIMSPTVLFFFLHFNEIELHY
                     GDTAWLGKQISWFVGYISFSVVLFNYCLYLVGRRESGFIATFVHNMDGRLLFIRSQLI
                     ASLIMSILYVFFFILVVLTGFQASPDYQIVMIILKSIYINAFMMVSLTFMASFRVTFQ
                     TASTIYSVLITVCMVSGIVSLKYNEGIVYWINQVNPIAIYSTILQSDQELSLMTIFFY
                     SIMLIISIISALTFKTEPVWSSQ"
     gene            complement(21609..22799)
                     /locus_tag="KJL94_001309"
     CDS             complement(21609..22799)
                     /locus_tag="KJL94_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBD4409732.1"
                     /translation="MINVYSNLMSAWPATMAMSPKLNRNMPTFSQIWDYERITPASAA
                     GETLKSIQGAIGEYFERRHFFNEIVTGGQKTLYEMIPPSAAKAFTEAFFQISSLTRDE
                     IITHKFKTVRAFNLFSLEQQEIPAVIIALDNITAADDLKFYPDRDTCGCSFHGSLNDA
                     IEGSLCEFMERQSLLLYWLQGKANTEISSEIVTGINHIDEILLALRSEGDIRIFDITL
                     PGAPGHAVLTLYGTKNKISRIKYSTGLSYANSLKKALCKSVVELWQSYICLHNFLIGG
                     YTDDDIIDSYQRHFMSCNKYESFTDLCENTVLLSDDVKLTLEENITSDTNLLNYLQQI
                     SDNIFVYYARERVSNSLVWYTKIVSPDFFLHMNNSGAININNKIYHTGDGIKVRESKM
                     VPFP"
     gene            complement(22780..23598)
                     /locus_tag="KJL94_001310"
     CDS             complement(22780..23598)
                     /locus_tag="KJL94_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HBD4409733.1"
                     /translation="MSKHELSLVEVTHYTDPEVLAIVKDFHVRGNFASLPEFAERTFV
                     SAVPLAHLEKFENKEVLFRPGFSSVINISSSHNFSRERLPSGINFCDKNKLSIRTIEK
                     LLVNAFSSPDPGSVRRPYPSGGALYPIEVFLCRLSENTENWQAGTNVYHYLPLSQALE
                     PVATCNTQSLYRSLSGGDSERLGKPHFALVYCIIFEKALFKYRYRGYRMALMETGSMY
                     QNAVLVADQIGLKNRVWAGYTDSYVAKTMNLDQRTVAPLIVQFFGDVNDDKCLQ"
     gene            complement(23600..24487)
                     /locus_tag="KJL94_001311"
     CDS             complement(23600..24487)
                     /locus_tag="KJL94_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McbB family protein"
                     /protein_id="HBD4409734.1"
                     /translation="MVLPDIKKGKDMINILPFEIISRNTKTLLITYISSVDITHEGMK
                     KVLESLRSKQGIISEYLLDKLLDESLIDKDKGKEFLITTGVINKTKTSPLWVNSVIIS
                     DVPHLFSNAREQWKSDGVFVSHIIDIKDNNINVSDSTLIWLHLENYHSDIVKRIYSKF
                     ESNPGVAFIQSYYLKESFRIDGVYSPDLGTPCHFCHIERWLSREEKSFRRNEMSWANL
                     LQLLKKYQMTLPALALGESERGFSYHLIKRRLQELTGTSLVKSHVDNFMSSVSADLIT
                     CILCKEPVIHWQACSCLER"
     gene            complement(24514..24723)
                     /locus_tag="KJL94_001312"
     CDS             complement(24514..24723)
                     /locus_tag="KJL94_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17"
                     /protein_id="HBD4409735.1"
                     /translation="MELKASEFGVVLSVDALKLSRQSPLGVGIGGGGGGGGGGSCGGQ
                     GGGCGGCSNGCSGGNGGSGGSGSHI"
     gene            25253..25705
                     /locus_tag="KJL94_001313"
     CDS             25253..25705
                     /locus_tag="KJL94_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4409736.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPIENKGIYAGNPARLIRIL"
     gene            complement(26108..26458)
                     /locus_tag="KJL94_001314"
     CDS             complement(26108..26458)
                     /locus_tag="KJL94_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBD4409737.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADLNSSTIIQSSSQSEQK
                     SDEETKKNAMKLIN"
     gene            complement(26461..27423)
                     /gene="parM/stbA"
                     /locus_tag="KJL94_001315"
     CDS             complement(26461..27423)
                     /gene="parM/stbA"
                     /locus_tag="KJL94_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBD4409738.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            27934..28860
                     /locus_tag="KJL94_001316"
     CDS             27934..28860
                     /locus_tag="KJL94_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBD4409739.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLF
                     LAGSAGLLQITENIRSEQCPGVTAAGRGAVSLENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVTHYGG"
     gene            28857..29168
                     /locus_tag="KJL94_001317"
     CDS             28857..29168
                     /locus_tag="KJL94_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409740.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARGGRFYRSRLASVKVYASPSPFTDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            29245..29928
                     /locus_tag="KJL94_001318"
     CDS             29245..29928
                     /locus_tag="KJL94_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD4409741.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGVELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            29929..30150
                     /locus_tag="KJL94_001319"
     CDS             29929..30150
                     /locus_tag="KJL94_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409742.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            30164..30598
                     /locus_tag="KJL94_001320"
     CDS             30164..30598
                     /locus_tag="KJL94_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBD4409743.1"
                     /translation="MYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            30643..31413
                     /locus_tag="KJL94_001321"
     CDS             30643..31413
                     /locus_tag="KJL94_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409744.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            31829..32254
                     /locus_tag="KJL94_001322"
     CDS             31829..32254
                     /locus_tag="KJL94_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4409745.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            32301..32723
                     /locus_tag="KJL94_001323"
     CDS             32301..32723
                     /locus_tag="KJL94_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBD4409746.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRYIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            32720..32911
                     /locus_tag="KJL94_001324"
     CDS             32720..32911
                     /locus_tag="KJL94_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409747.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            33946..34176
                     /locus_tag="KJL94_001325"
     CDS             33946..34176
                     /locus_tag="KJL94_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409748.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            34228..35589
                     /locus_tag="KJL94_001326"
     CDS             34228..35589
                     /locus_tag="KJL94_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBD4409749.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            35637..36200
                     /locus_tag="KJL94_001327"
     CDS             35637..36200
                     /locus_tag="KJL94_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4409750.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            36226..36462
                     /locus_tag="KJL94_001328"
     CDS             36226..36462
                     /locus_tag="KJL94_001328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409751.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMLLFLLLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            37105..37644
                     /locus_tag="KJL94_001329"
     CDS             37105..37644
                     /locus_tag="KJL94_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBD4409752.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            37707..37940
                     /locus_tag="KJL94_001330"
     CDS             37707..37940
                     /locus_tag="KJL94_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBD4409753.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            38006..39964
                     /locus_tag="KJL94_001331"
     CDS             38006..39964
                     /locus_tag="KJL94_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD4409754.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVTDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDPLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMYAPRPQTDTTERTDNLADAA"
     gene            40019..40453
                     /gene="psiB"
                     /locus_tag="KJL94_001332"
     CDS             40019..40453
                     /gene="psiB"
                     /locus_tag="KJL94_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBD4409755.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            40450..41169
                     /locus_tag="KJL94_001333"
     CDS             40450..41169
                     /locus_tag="KJL94_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBD4409756.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(41181..41369)
                     /locus_tag="KJL94_001334"
     CDS             complement(41181..41369)
                     /locus_tag="KJL94_001334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HBD4409757.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            41449..41607
                     /locus_tag="KJL94_001335"
     CDS             41449..41607
                     /locus_tag="KJL94_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD4409758.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            42298..42504
                     /locus_tag="KJL94_001336"
     CDS             42298..42504
                     /locus_tag="KJL94_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBD4409759.1"
                     /translation="MYIPQNFLPRSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSTDDGG"
     gene            42529..42816
                     /locus_tag="KJL94_001337"
     CDS             42529..42816
                     /locus_tag="KJL94_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409760.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            42935..43756
                     /locus_tag="KJL94_001338"
     CDS             42935..43756
                     /locus_tag="KJL94_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBD4409761.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(44053..44655)
                     /locus_tag="KJL94_001339"
     CDS             complement(44053..44655)
                     /locus_tag="KJL94_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBD4409762.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            44986..45369
                     /locus_tag="KJL94_001340"
     CDS             44986..45369
                     /locus_tag="KJL94_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBD4409763.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDEG"
     gene            45503..46180
                     /locus_tag="KJL94_001341"
     CDS             45503..46180
                     /locus_tag="KJL94_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBD4409764.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVFCFYAL
                     GYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEILNVLSE
                     YMGVKKM"
     gene            46268..46378
                     /locus_tag="KJL94_001342"
                     /pseudo
     CDS             46268..46378
                     /locus_tag="KJL94_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
     gene            46432..46762
                     /locus_tag="KJL94_001343"
                     /pseudo
     CDS             46432..46762
                     /locus_tag="KJL94_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT        11684 a        11141 c        12623 g        11314 t
ORIGIN      
        1 aatgagcagg cgactcagga actggcatca acaagtacca ggctatctgg tggaatgtat
       61 gctgatgctc atgctgaatg gggggttaaa cctaaaattc ttggtgttgg tggtggggta
      121 ggcatcagag gtggatttaa ggggagcctg gatgcaacgg atgatgactc acattcagca
      181 agtagtggca gcagggctac ccatgatgct cgccatgata tcgatgctaa agccagtaag
      241 gactttaaag aggccagcga ttactttacc agtcgcaaag tcagtgaatc cggcagccat
      301 actgacaata atgccgattc ccgcgtggac cagttgtctg cagccctgaa ctcagcaaaa
      361 cagagttacg accagtacac gacgaatatg acccgcagcc atgaatatgc ggaaatggcc
      421 tcccgtactg aaagcatgag cgggcagatg agtgaagact tgtcgcaaca gtttgcacag
      481 tatgtgatga aacgcgcacc gcaggatgca gaagctattc tgacaaatac cagttcgccg
      541 gagattgcag aacgccgtcg ggctatggcg tggtcttttg tgcaggaaca ggtacagcct
      601 ggtgttgata atgcctggcg tgaatcccgt ggggatattg gtaaaggaat ggagagcgta
      661 ccttcgggtg gtggcagcca ggatattatt gctgaccatc aggggcatca ggccattatt
      721 gatcaaagaa cgcaggacag taatatccgt aatgatgtaa aacatcaggt tgataatatg
      781 gtcacagaat ataaagggaa tatcggagat actcagaata gtattcatgg cgaagaaaat
      841 attgtagaca gacaatattc agagttaaaa aataatcaca aacaggaaga aattcagcaa
      901 aacaacagat ataatgaaga aaataaacga cagatattaa tgccaacacc gtctgaagat
      961 gcgttaaaac agatggtgga tgataagaaa gaacgattga aggggccatt atgatcactc
     1021 actatgatat aaaaatggag atgcagaaac taaaggaggt actctctgta gagggtgtta
     1081 atatcccatc attattacaa gtcataaagc cgggaactta tgtgttttta tgggttttat
     1141 tatggccaac ttttctgcgt ttagtatcgg ttaaaagtga tgtcagggat gttggctttg
     1201 atatatgtgc tagtggaatg atggggttcc tgctttttgt ggctattaca aatggaatga
     1261 tgttatacct tgcgattcct gatagtttca gaaaggattc aaaaataata aattttatgt
     1321 attctaagag taagacatat attttgttgt ttttgattgt tttttctatg gtttcgttta
     1381 tgcattctat cttatatgtt tttgcactaa tgattacatt catattattt ttcttggtgt
     1441 ataccataga tataaatcgt tataatctct cagctatagc ctcagtaata ggactattca
     1501 aaaaagagtc tgttagttaa aaggatattt tggatgaaaa caaaaaaatt gatgatggtt
     1561 gcactggtca gttccactct ggccctttca gggtgtggtg cgatgagcac agcaatcaag
     1621 aagcgtaacc ttgaggtgaa gactcagatg agtgagacca tctggcttga acccgccagc
     1681 gaacgcacgg tatttctgca gatcaaaaac acgtctgata aagacatgag tgggctgcag
     1741 ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg tggtgacttc tccggataaa
     1801 gcctactact ggattcaggc gaatgtgctg aaggccgata agatggatct gcgggagtct
     1861 cagggatggc tgaaccgtgg ttatgaaggc gcagctgttg gtgcagcgtt aggtgccggt
     1921 attaccggat ataactcaaa ttctgccggt gccacactcg gtgtaggcct tgctgctggt
     1981 ctggtgggta tggctgcaga tgcgatggtg gaagatgtga actataccat gatcacggat
     2041 gtacagattg cagagcgtac taaggcaacg gtgacaacgg ataatgttgc cgccctgcgt
     2101 cagggcacat ccggtgcgaa aattcagacc agtactgaaa cgggtaacca gcataaatac
     2161 cagacccgtg tggtctcaaa tgcgaacaag gttaacctga aatttgaaga ggcgaagcct
     2221 gttctcgaag accagctggc caaatcaatc gcaaatattc tctgaacctc gtcaggaggc
     2281 tgatctgacc ggcctccttg ctgacgcctc gcgttgctcg ttgtccaacc ccgggatgta
     2341 acaaaataaa ttttgtcacc tcccggcgtt tccgaaataa atatgaaaac actgagcggt
     2401 tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga agccgggatc ttccggagaa
     2461 agtcattatg acatcagaca atatcgctga atatgttggt gagaataatc aatttactga
     2521 gaagcctgta actgaatacc ccccggtgat acaggataat acagtgacca ctaaagacta
     2581 tctgtacagc aataatattg atactgacct tttagtggtt atcggagggc ttattgttat
     2641 attgtttctc atgatgctta tcatgtactt tattaattct ctcaggcata aggaattaac
     2701 tgaaatactg acaaaaatat cttttcctga aaaatccggg agcaatttgc ttaaaccgga
     2761 ggcggtcagc tgtaatttgt tgtccctgtg tgtgctggca ccggtattct gtacacccag
     2821 aaagctggct gaagtttcca gggagatcat cgtggcgagt gcgcaggaaa ttgcatggac
     2881 gccggggagc agtcgtattg atggccacca ggcggagaat atcacctggg cgatttcatc
     2941 gcagtctcct gccgataagt atgatctcat caataaaacg tcctgtgttt ctcctgacag
     3001 tggccgtttt tatgaaccgg acacagttct ggatcaccgt gaacgggtac gcctttttat
     3061 tcagatccag tttccgcatg ggagtgactg gtctgaagta ttgcgcctga ctgacattgt
     3121 tacagagaag gtctcttccg gcaggactgc aggcgggcat caccaccggc atacagggta
     3181 cacctggtct gtttctgtga attaacccca catcatcctt ttctgccctg ttccagattt
     3241 tcccggagtt tcatcatgag ttttaacgca aaggatatga cccagggcgg tcagattgcg
     3301 tccatgcgta tccgcatgtt cagccagatc gccaatatca tgctttactg cctgtttatt
     3361 tttttctgga tcctcattgg tctggtctta tgggtaaaaa taagctggca gacgtttatc
     3421 aacggctgta tttactggtg gtgtaccacg ctggaaggca tgcgggattt aataaaatcc
     3481 cagccggtct atgagatcca gtattacggc aaaaccttcc ggatgaacgc tgcccaggta
     3541 ctgcatgata aatatatgat ctggtgcgga gagcagctat ggtccgcatt cgttctggcc
     3601 acagttgtgg cactggttat ttgcctgatc accttctttg ttgtctcctg gattctgggg
     3661 catcagggga aacagcagag tgaaaatgag gtgaccggcg ggcgtcagct gacggataac
     3721 ccgaaagaag tggcccggat gctgaaaaaa gacggcaagg actccgatat ccggattggc
     3781 gacctgccga ttatccggga ttccgaaatc cagaacttct gtctgcacgg cacggtcggg
     3841 gccggtaagt cggaggttat ccgtcgtctg gccaactacg cccgtcagcg cggtgatatg
     3901 gtggtgattt atgaccgttc aggggaattt gttaaaagtt actatgatcc ctccatcgat
     3961 aaaatcctga atccgctgga tgcccgctgt gccgcatggg atttgtggaa ggagtgcctg
     4021 acacagccgg attttgataa taccgcgaac acgctgatcc ctatgggtac aaaagaggac
     4081 ccgttctggc agggttcagg acgtaccatt tttgcggaag cggcgtacct gatgcgtaat
     4141 gaccccaacc gcagctacag caaactggtg gacacactgc tttccatcaa aattgaaaaa
     4201 ctgcgtacct tcctgcgtaa ttcaccggca gccaacctgg tggaagagaa aattgagaaa
     4261 acggcgattt ccatccgtgc tgtgctgacc aactacgtga aagccatccg ttacctgcag
     4321 gggattgagc ataacggtga gtcttttacc atccgtgact ggatgcgtgg tgtccgggaa
     4381 gatcagaaaa acggctggct gtttatttcg tcgaacgccg acacccatgc ctccctgaag
     4441 ccggtgatct ccatgtggct gtccattgcc attcgtggtc tgctggcaat gggagaaaac
     4501 cgtaaccgtc gtgtgtggtt tttctgtgac gagttaccca cgttacacaa actgccggac
     4561 ctggtggaga tcctgccgga agcccgtaag ttcggtggct gttatgtgtt tggtatccag
     4621 tcctatgccc agctggaaga tatctacggt gagaaagcgg cggccacgct gtttgacgtc
     4681 atgaacaccc gtgccttttt ccgttccccg agccataaga ttgcagagtt cgctgccggt
     4741 gaaattggtg agaaagagca cctgaaagcc agcgagcagt attcctacgg tgctgacccg
     4801 gtacgtgacg gggtatcgac cggtaaggat atggagcgtc agacgctggt cagttattcc
     4861 gacattcagt ctctgccgga tctgacctgc tatgtcactc tgcccggacc gtatccggca
     4921 gtaaaactct ctctgaaata tcaggcacga ccgaaggtcg ctccggagtt tattccgcgt
     4981 gacatcaacc cggaaatgga gaaccgtctg agtgccgtac ttgctgcaag ggaagcagaa
     5041 ggtcgccaga tggccagcct cttcgaaccg gaggttgcct ccggagaagg cgtgactcag
     5101 gctgaacagc cacaacagcc acaacagcca caacagccac aacagccaca acagccacaa
     5161 cagccacaac agccacaaca gccacaacag ccacaacagc cagtgtcttc tgtcatcaat
     5221 gataagaagt cagatgcagg tgtgagtgtt ccggcagggg ggatcgagca ggagctgaaa
     5281 atgaaaccgg aagaagagat ggaacagcaa ctgccacccg ggatcagtga atccggtgaa
     5341 gtggtggata tggccgctta tgaggcatgg caacaggaaa atcatccgga catccagcag
     5401 cagatgcagc gtcgtgaaga ggtgaacatt aatgtgcacc gggagcgtgg ggaggatgtt
     5461 gagccgggag atgatttctg atgttgagtt tcagcgttgt taaatcggca ggcagtgccg
     5521 gaaactatta caccgacaag gataattact atgtgctggg cagcatggga gaacgctggg
     5581 ctggtcaggg ggctgaacag cttgggctgc agggcagcgt cgataaggat gtttttaccc
     5641 gtcttctgga gggcaggctg ccggacgggg cggatttaag ccgcatgcag gatggcagta
     5701 ataagcatcg tcccggctac gacctgacct tctccgcccc caaaagtgtc tccatgatgg
     5761 ccatgctcgg gggggataaa cgtcttattg atgcacataa ccaggccgtg gattttgctg
     5821 tccgtcaggt ggaggcgctg gcttccacac gggtgatgac ggacggacag tcagaaacgg
     5881 tgctgaccgg taatctggtg atggcactgt ttaaccatga caccagtcgc gatcaggacc
     5941 cacagttaca cacgcatgta gtggtggcta atgtcacgca gcataacggc gagtggaaga
     6001 cactgagcag tgacaaagtg gggaaaaccg gattttccga gaacgtgcta gcgaaccgga
     6061 ttgcgttcgg gaaaatatat cagagtgagt tacgtcagcg cgttgaggcg ctgggttatg
     6121 aaaccgaagt ggtcggtaag cacggtatgt gggagatgcc gggcgtaccg gtggaggcct
     6181 tttccagtcg ttcacaggct atcagggagg ccgtcggtga agacgcctcg ctgaaatccc
     6241 gggatgtggc ggcactggat acgcgaaaat ccaaacagca cgtcgatccg gaaatcagaa
     6301 tggctgaatg gatgcagacg ctgaaggaaa ccgggttcga catccgggca tatcgtgacg
     6361 cggcggatca gcgtgcggag atccgcacgc aggcgcccgg gcctgcttca caggacgggc
     6421 cggatgtgca gcaggcggtg acacaggcga ttgccggatt aagtgaacga aaagtgcagt
     6481 tcacgtatac ggacgtgctg gccaggacgg tcggcatact gccgccggaa aatggtgtga
     6541 ttgaacgggc acgcgccggt atcgatgagg ccatcagccg tgagcagctt atccccctcg
     6601 accgtgagaa ggggctgttc acatcaggaa ttcatgtgct cgatgagctg tccgtccggg
     6661 cactcagtcg tgacatcatg aaacagaacc gggtgaccgt acatccggag aaaagtgtcc
     6721 cccggacggc tggttacagc gatgcggtca gtgttctggc acaggaccgt ccgtcgctgg
     6781 ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga gcgggtggct gaactggtca
     6841 tgatggcccg ggagcagggg cgggaggtgc agattatcgc tgctgaccgt cgctcgcaga
     6901 tgaacctgaa gcaggatgaa cggctgtccg gtgagctgat aaccggacgt cgtcagctgc
     6961 aggagggcat ggtcttcacg ccgggcagta ctgttatcgt cgaccagggc gaaaaactct
     7021 ccctgaaaga gacgttaacc ctgctggacg gtgccgcacg tcataacgta caggtcctga
     7081 taaccgacag cgggcagcga accggtacag gcagtgcgct gatggccatg aaggatgccg
     7141 gggtgaacac gtatcgctgg cagggcggag aacagcgacc ggcaaccatc atcagtgaac
     7201 cggaccggaa tgtccgctat gaccggctgg ccggagattt tgcggccagc gtgaaagccg
     7261 gagaagagag cgtggcacag gtcagcgggg tacgggaaca ggccatactg acacaggcca
     7321 ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc ggaggtgacc atgaccgccc
     7381 tgtcaccggt ctggctggac agccggagcc gttatctgcg ggatatgtac cgaccgggga
     7441 tggtgatgga gcagtggaac ccggagacac gcagtcatga ccgctatgtt atcgaccggg
     7501 tgacggcgca gagtcacagc ctgaccctgc gggatgcgca gggcgaaacg caggtggtgc
     7561 gtatttcctc cctggacagc agctggtcgc tgttccggcc ggaaaaaatg ccggtggcag
     7621 acggcgagcg actgagggtg acagggaaaa ttcccggact ccgcgtctcc ggcggtgacc
     7681 gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt tgttgtgccg gggcgggctg
     7741 aaccggccac cctgcctgtg gctgattcac cgttcacggc actgaagctg gagaacggct
     7801 gggtggaaac gcccgggcat tccgtcagtg acagtgcgac ggtgtttgcc tccgtcacac
     7861 agatggcaat ggacaacgcc accctgaacg gtctggcccg cagcggtcgt gatgtccggc
     7921 tgtattcctc actggatgaa acccgtactg cggaaaaact tgcccgccat ccctccttta
     7981 cggtggtttc tgagcagata aaggcgcggg ccggtgagac atcgctggaa accgctatca
     8041 gtctgcagaa aaccgggctg cacacgccgg cacagcaggc cattcatctg gcccttcctg
     8101 tgctggaaag taaaaacctg gccttcagca tggtggacct gctgacagag gcgaagtcgt
     8161 ttgctgcaga aggaaccggt tttactgaac tgggagggga aatcaatgcg cagataaaac
     8221 gcggtgactt actgtatgtg gatgtggcaa aaggctatgg cacaggcctg ctggtttccc
     8281 gtgcgtcgta tgaggcagaa aaaagtattc ttcgccatat tctcgaaggt aaggaggcgg
     8341 tcacgccgct gatggagaga gtacccggcg aactcatgga gacgttaacg tcgggacagc
     8401 gtgccgccac ccgtatgata ctggaaacgt ccgaccgttt cacggtggta cagggttatg
     8461 ccggtgtggg taagaccaca cagttccggg cggtgatgtc agccgtgaac atgctgccgg
     8521 cgagtgagcg tccccgagtt gtggggctgg ggcccacgca ccgtgcggtc ggtgagatgc
     8581 gcagcgccgg cgtggatgca cagacgctgg cgtcctttct gcatgacacg cagctgcagc
     8641 agcgcagcgg agaaacgccg gatttcagca acacgctgtt cctgctcgat gagagctcaa
     8701 tggtgggcaa taccgacatg gcacgggcat acgccctgat tgcggccggt ggcggtcgtg
     8761 ccgtggccag tggtgacacg gaccagctgc aggccatcgc gcccggtcag cctttccgtc
     8821 tccagcagac gcgcagtgct gccgatgtgg tcatcatgaa ggagattgtg cgtcagacgc
     8881 cggaactgcg ggaggcggta tacagcctga ttaaccggga tgtggaaagg gcactgtccg
     8941 ggcttgagag tgtgaaaccg tctcaggttc cccgtcagga gggcgcatgg gcaccggagc
     9001 actccgtgac ggagttcagt cacagccagg aagcgaaact ggcagaagcg cagcagaagg
     9061 cgatgctgaa aggtgagact tttccggatg tccccatgac actgtatgaa gccattgtcc
     9121 gcgactatac cggcaggaca ccggaagcac gggagcagac gctgattgtc acgcacctga
     9181 atgaggaccg gcgcgtactg aacagcatga ttcatgatgc acgggaaaag gccggtgagc
     9241 tggggaaaga gcaggtcatg gtgcctgtcc tgaacacagc gaatatacgt gacggggagc
     9301 tgcgtcgtct ctccacctgg gagaataacc cggatgccct tgccctggtg gatagtgtgt
     9361 atcaccggat tgccggtatc agcaaggatg acgggctgat aaccctggag gatgcggaag
     9421 gtaacacgcg gctgatttcg ccccgggagg cggtggctga aggtgtcaca ctgtacaccc
     9481 cggacaaaat ccgggtgggg accggcgacc ggatgcgctt cacgaagagt gaccgggagc
     9541 gcggttatgt ggccaacagc gtctggacgg tgacagcggt ttccggtgac agtgtcacgc
     9601 tgtcagacgg acagcagacc cgggtgattc gccccggcca ggagcgggca gagcaacata
     9661 ttgacctggc ttatgccatc accgcccacg gtgcgcaggg ggcgagtgaa acctttgcca
     9721 tcgcgcttga aggcacggaa ggtaaccgga aactgatggc cggctttgag tcagcctacg
     9781 tggccctgtc gcgtatgaag cagcatgtgc aggtgtacac cgataaccgt cagggctgga
     9841 cggatgccat taacaatgcc gtacagaaag gaactgccca cgatgtgctg gagccgaaac
     9901 cggaccggga ggtcatgaat gcgcagcggc tgttcagtac ggcacgggaa ctgcgggacg
     9961 tggcggcagg ccgtgccgtt ctccgtcagg cagggctggc cgggggagac agccctgcac
    10021 ggtttattgc accgggacgt aaatatccgc agccgtatgt ggcactgccg gcgtttgacc
    10081 gtaacggcaa gtcagctggt atctggctga acccactgac cacggatgac ggaaacgggc
    10141 tgcggggatt cagtggtgaa ggacgggtga aaggcagcgg ggatgcgcag ttcgtggccc
    10201 tgcagggcag ccgtaacgga gagagcctgc tggctgataa tatgcaggat ggcgtccgga
    10261 ttgcccgtga taatcctgac agcggtgtgg tggtgagaat tgccggtgaa ggtcgtccgt
    10321 ggaatcccgg tgccatcacc ggtggccgcg tgtgggggga tatcccggat aacagcgtcc
    10381 agccgggggc cggaaatggc gaaccggtca cggcagaggt actggcacag cggcaggctg
    10441 aagaggccat ccgccgtgaa acggaacgcc gcgcagatga aattgtcagg aaaatggcag
    10501 agaacaaacc tgacctgccg gatggcaaaa cagagctggc tgtcagggat attgccggac
    10561 aggagcgtga ccggtctgcc atatctgaac gggaaaccgc actgccggag agtgtgctgc
    10621 gtgaatcaca acgggagcgg gaagcggtcc gggaggttgc ccgggaaaat ctgctgcagg
    10681 agcgtctgca gcagatggag cgggatatgg ttcgtgacct gcagaaagag aaaaccctgg
    10741 gcggagactg atacaggaag acaaacactg atgacaacgg ataacacgaa cacgacacgt
    10801 aacgattcac tggctgcacg gaccgatacc tggttgcagt cgtttctggt ctggtcaccc
    10861 ggacagcggg atatcatcaa aacggtggcg ctggtgctga tggtactgga ccacatcaat
    10921 ctgatattcc agctgaagca ggaatggatg tttctggccg ggcgaggagc ctttccgctg
    10981 tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc atatccggca accggccatt
    11041 aaccggctgt ggggatgggg aattattgcc cagttcgcat attatctggc cggctttccc
    11101 tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt
    11161 gaaacgcgca gtgggtggcg tacagcagcg gccattcttc tgatggccct gtgggggcct
    11221 ttgtccggca ccagttacgg cattgccggg ctgctgatgc tggcggtcag ttaccgactg
    11281 taccgggcgg aagacagagc ggaacgtctg gcgctgcttg catgcctgct ggccgttatt
    11341 ccggcactta accttgcctc cagtgatgca gcggcggtgg ccggtcttgt aatgacggtg
    11401 ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac cccgtttctg gcccggggat
    11461 tttttcccgg tattctacgc ctgtcatctg gctgtgctgg gcgttctggc gttgtgactg
    11521 gtatgatact gacgctgatt ctgattatta ataaacaaca taacacagga tatttatgaa
    11581 aattacggat cacaaactct ctgaaggtat tgccctgaca tttcgtgttc ctgaaggaaa
    11641 tataaaacat cctttgatta tcttatgcca tgggttttgc ggtatcagga atgttctgct
    11701 cccttgcttt gcgaatgcct tcacagaagc aggattcgca acgattactt ttgattatcg
    11761 aggattcggt gagagtgatg gagaacgagg gcgtctggtt cctgcaatgc agaccgaaga
    11821 tattatttca gttatcaatt gggcagaaaa gcaggaatgt attgataatc agcgtattgg
    11881 gttatgggga acatcgctgg gaggggggca tgtcttcagt gcagctgcgc aggaccaacg
    11941 cgtaaaatgt attgtcagcc agctggcttt tgctgacggt gatgttctgg ttaccggaga
    12001 gatgaatgaa tcggagagag cgtcttttct gtccacgctg aataaaatgg cagagaagaa
    12061 gaaaaacacc gggaaagaga tgtttgtggg ggttacccgg gtactgagtg ataatgaatc
    12121 aaaggtattc ttcgagaagg taaaaggtca gtatcctgaa atggatataa aaataccttt
    12181 ccttacggtt atggagacgt tgcaatataa accggcagag agcgcagcca aagtacagtg
    12241 cccagttttg attgttattg ccggccagga tagtgttaat ccaccagaac agggaaaagc
    12301 cttatatgat gctgttgctt ctggtactaa ggaattatac gaggaagctg acgcatgcca
    12361 ttatgatatt tatgaaggcg cgttttttga acgtgtggcg gcagtacaga cgcaatggtt
    12421 taaaaaacat ctttaattgt cggtattaat atgtcagagg tcgttgaggt cagcttaata
    12481 tcatagtgag tagtttcaac gacctggagg acatattttc acaaaggtca gggcaatcat
    12541 gacagagcag aaacgaccgg tactgacact gaaacggaaa acggaagggg aaacaccgac
    12601 ccggagccgg aaaaccatca tcaatgtcac cacgccacca aaatggaagg tgaaaaagca
    12661 gaaactggcg gagaaggctg cccgggaagc agagctgaca gcaaaaaaag cgcaggccag
    12721 acaggcgctg tccatttatc tgaacctgcc ctcgctggat gaggccgtga acaccctgaa
    12781 accctggtgg ccgggattat ttgacggtga cacaccccga cttctggcct gcggtatccg
    12841 ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg
    12901 cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc
    12961 ctgccggtat gacacggaag ggtatgtgac ggagcatatt tctcaggagg aagaagtgta
    13021 tgcggcagag cgtctggata aaatccgccg ccagaaccgg ataaaggcag aacttcaggc
    13081 cgtgcttgat gaacaataaa aaagcctcct tcacactaaa ggaggcggaa aaataaggct
    13141 tctctgaaac cttcttccat acccggcaga agaaggttat aaaaatttat aacacattca
    13201 ccggcctgga ttaaaggggg aaggaaaatg aacggaacag ggcgtgattc gcaaaactcc
    13261 ggggtatggc gctaccggca ggtcggacgc cgggctgtcc ggctggaagt caggggggac
    13321 tggccgggag ccgctgaggc atggctacgg gctgcgcagt ctgctccccg gacagactgg
    13381 cagcagtttg cccgaaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa
    13441 cgaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca tgagatttgc
    13501 tttttttaat atccggtcgg gaagtatgac cggacagttc tgaatagcac agagtttttt
    13561 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tgttattcag
    13621 tgtgtgctgc gatattcagg attacctgaa atacaggtgc catttatctg atatggagaa
    13681 taacatgagg aaatatattc ctctggtatt atttatcttt tcatggccgg tattgtgtgc
    13741 agatattcat ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc
    13801 acggaaggca gtgcggatca ggctgataaa tattgatgca ccggaaaaga aacaggatta
    13861 tggccggtgg tcaacggaaa tgatgaaatc actggttgca ggaaaaacgg ttaccgtcac
    13921 atattttcag cgagatcgtt atggtcgtat tctcgggcag gtttatgcac cagacggaat
    13981 gaatgttaat cagtttatgg tccgggccgg ggctgcctgg gtatatgagc agtacaacac
    14041 agatcccgtg ctgcctgtac ttcagaacga agccagacaa caaaaacgtg ggctctggtc
    14101 agatgctgat ccggttcccc cctggatatg gagacaccgt aaataaagcg atggcaatct
    14161 tatcattcat ctgttctcag cgggaataaa aatggcaaaa acaaaacagg aatggctgta
    14221 tcagttacgg cgatgttcat cagtcaatac gctggaaaaa atcattcata aaaacagaga
    14281 cagtttatcg accagtgaac gtgaatcatt taattcagca gcagaccacc ggttagcgga
    14341 attaatcacc ggaaagttat atggcaggat accaaaagag atatggaaat atgtaaggta
    14401 ataattcagc aataattaat taccaggagc agataaacaa tgaagctgat tatttttatt
    14461 ctcattgttc tgattattgc agcgttactt atccggatta tcctcagaag tgtaaatcag
    14521 cattctcctt tgctgatgca attgcatgcg gcaggtatac ggaccggtga tgcagagcga
    14581 atactgtccg gcggtgaata ctggcaacgt cagaagacgc tgctgacaga aagggaagtc
    14641 agttttatga aaggactgtt cagaattgtg gatatgaagc ggtggtatct gtgtccgcag
    14701 gtacgggtcg cagatatcgt ccagctgaac gggaatatcc ggccacgatc acgccagtgg
    14761 tggcagttat tcaggatggt gtctcagtgg catgttgatg tggttatcgt tgagcggcgt
    14821 tcgttcagta ttgttgcagc agtagagctg gatgatgcca gccatttacg acctgaacgc
    14881 agacgccggg atattcttct ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga
    14941 agccacgatg ccagaaaact gctgcagatg accggagaat ggctgaatac aacaggggct
    15001 gcccagcagt caccggaaca tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc
    15061 ggaaacggga aaaagcaagt tttccccgct cccggcgttt ccataactga aaaccatact
    15121 atttcacagt ttaaatcaca ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca
    15181 gatcttcgtc acaattctca agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac
    15241 gatccctgtt tgagtattga ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct
    15301 catcaggtac aaagcgagca tacagaaaag gtaatcctct gacgcttgca gaacgtcagc
    15361 aggcatccct ggcaagaaaa cgagctacac ataaagaact cagagtgttc attcctgctg
    15421 cgttaaaagc gcaattgcag gtgatgtgtg aagctgaggg cgttacccag gctgagatga
    15481 ttgccgaact gataaagcag aagagtgctt ttagctaact aaaatgtagc gacatcacat
    15541 tcttgcctgt ttctggtgtg cgtgatagat tgctgatcgt ttaaggaatt ttgtggctgg
    15601 ccacgccgta aggtggcagg gaactggttc tgatgtggat ttacaggagc cagaaaagtg
    15661 aaaaccccga taatcttctc taactttggc gagtacgaaa gattaacggg gctaacaaga
    15721 aactgcatag aagctgttgc tctatgcggg gagtatagtt atatgcccgg aaaagttcaa
    15781 gacttctttc tgtgctcact ccttctgtgc aacataagtg caggaaggtg tgactgatct
    15841 tcaacaaacg tattaccgcc aggtaaagaa cccgaatccg gtgtttacac cccgtaaagg
    15901 tgccggaacg ctgaagttct gcgaaaaact gatggaaaag gcggtgggct tcacctcccg
    15961 ttttgatttc gccattcatg tggcgcatgc ccgttcgcgt ggtctgcgcc ggcgcatgcc
    16021 accggtgctg cgtcgacgtg ccattgatgc gctgctgcag gggctgtgtt tccactatga
    16081 cccgctggcc aaccgcgtcc agtgctccat caccacgctg gccattgagt gcggactggc
    16141 gacagagtcc ggtgccggaa aactctccat cacccgtgcc acccgtgccc tgacgttcct
    16201 ggctgagctg gggttgatta cctaccagac ggaatatgac ccgcttatcg gctgctacat
    16261 tccgaccgac atcacgttca caccggcact gtttgccgct ctcgatgtgt ctgaggatgc
    16321 ggtggcagct gcgcgccgca gtcgtgttga atgggaaaac agacagcgca aaaagcaggg
    16381 gctggatacc ctgggtatgg atgagctaat agcgaaagcc tggcgttttg tgcgtgagcg
    16441 tttccgcagt taccagacag agcttaagtc ccgtggaata aaacgtgccc gtgcgcgtcg
    16501 tgatgcgaac agggaacgtc aggatatcgt caccctggtg aaacggcagc tgacgcgtga
    16561 aatctcggaa gggcgcttca ctgctaatgg tgaggcggta aaacgcgaag tggagcgtcg
    16621 tgtgaaggag cgcatgattc tgtcacgtaa ccgtaattac agccggctgg ccacagcttc
    16681 cccctgaaag tgacctcctc agaataatcc ggcacgcgcc ggaggcttcc gcacgtctga
    16741 agcccgccag cgcacaaaaa atcagcacca catacaaaaa acaatctcat gatccagctt
    16801 ctggtgcatc cggtcccccc tgttttcgat acaaaacacg cctcactgac ggggaatttt
    16861 gcttatccac attaaactgc aagggacttc cccataaggt tacaaccgtt catgtcataa
    16921 agcgccatcc gccagcgtta cagggtgcaa tgtatctttt aaacacctgt ttatatctcc
    16981 tttaaactac ttaattacat tcatttaaaa agaaaacctg ttcactgcct gtcctgtgga
    17041 cagacagata tgtacctccc accgcaagcg gcgggcccct accggagcca ctttagttac
    17101 aacactcaga cacaaccacc agaaaaaccc cggtccagcg cagaactgaa accacaaagc
    17161 ccctccctca taactgaaaa gcgccgccgt cccgcccaaa gggcgggaac aacatcgctt
    17221 ttaattatga atgttgtaac tatactccgt catcgctgtc agtcttctcg ctggaagtat
    17281 tgagtacacg ctcgtaagcg gccctgacgg cccgctaacg cggagatacg ccccgacttc
    17341 gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa gctctctcat ggctgaaaac
    17401 gggtatggtc tggcagggca agggatgggt aaggtgaaat ctatcaatca gtaccggctg
    17461 acgccgggct tcggcggttt tactccggta tcacatgtaa ccacagcgtg ccgccttcca
    17521 tgccgctggc gcggcataag catatgttgg tggctgtgtc tgaaaggtta tatactctac
    17581 ataaaacctg acaacacgca gattgccagg caaatgagca ttatgcatcc ggttatcgcc
    17641 tgcctgtgaa acaatatcac cggggcgaat atcgatactc tgactcgtta tacccatgcg
    17701 tgtggcatca aaaaacctgc aattgccctt tattgaatga aagggggagt caattatgaa
    17761 tagcaccaca cattacgaaa acgccaattt cctgcgcgag ctggccgaaa gcctgcctcg
    17821 tatttttcca gaaggcagta cagacaagtc tgcactattg caacgcctcg ctaatgaaga
    17881 actggcacgg gcggaatacg acgaacagat ccgagcgaaa gttgccgctg cccgcgccga
    17941 taagcggccg ggaatgagtt ccgcgcaact gcgccagcaa ctacagggac gttatcagga
    18001 actgcgcaat gagttatgaa atagaatggc gaccaggtgc gattgaggat ataacggcgt
    18061 tgtttgaata tctggtcgag aatgcatctc tttgggatgc aaggaacgtg acagagcgtc
    18121 tgatcactgc aaccgatcgc ctggctgatt accccaggct gtatgaaacg gatgaacgct
    18181 atggtgaagg ggttcgccgt attagcttac ttggacagca tgtactttat gaagtgaatg
    18241 atgaggccag tcgtgtcaat atcctggcgg ttgtaggcca gcgtcagaat ccccgattca
    18301 ttctttgagt gtccggcttg gaactccatc ttcagagaat cgatcaacgt tctttagctc
    18361 cttccactaa cagccgacct atctcaaaca ccgcagctga ccgtcccgac ttcaccgctt
    18421 cggctttctg tccctcaaca aaccagttat taccgtccgt tcggaccaac tcaaacatcc
    18481 atgacatatc gtaaacgtgt tccgggatca gcgacccttc tccggccaga atacccgccg
    18541 ttatggagtg gttgcccccg tgcacaaagc caatacgcca gggcaaccac aggtcaacat
    18601 agtggttgtt tttatcttgc ttccacggct tccccttatg ggttccaatg cgtgaaacgt
    18661 tatcgatata gcgtggtagg ctccaaggcc acggcagcac tatgtcgctt gccagcgaca
    18721 acggataatc agcgccgttc atctcacact ctttcatctt ttcgtaagat tgtatttggg
    18781 taagtccctt aaagaagaac tcctgcgggg tcatatccgg gcgggcatct gttccctcct
    18841 cagctaccgc cagcaagaaa tcactctgta ctggccgcac gatagcgcga atgagatctg
    18901 gtagcgcctt aacgggtagt ttctctgcca cagaaagcag atgattgaag cgtttttttt
    18961 cattggctat agttgcttta tctttcttgt ttcggttcca gaacatcact gtctgtagcc
    19021 ctccttatcg tgatcaggat gatggttttc attctaatgg tgtagtagtt tcacgtcacg
    19081 cgcacgtgca tattcttcga aagcttcggc gttagccggt caaccagggg gttgaggccc
    19141 aaccgtacga gatcgtacgc taagttcatt ttttgagcag aagaggtgag taaagaaaac
    19201 gagggttttg tttactcttc ccgtcaatgc cagtagccat gcggtttcag ggactttcag
    19261 tgttaataaa ctcggtaatc aaatcaacgt aaacctgcct aacaacgcca cgactttatt
    19321 gacttaaaat catatagtta gcttcttagc gtacgtttcc gctccattgg gcttcaaacc
    19381 ccacacccaa tgacatgaga aaagctggat ctgattctca aaatgctggg atacccatcg
    19441 agatggagaa ctcagtcaat gttccattct ttctgaaata tgtgtgggga cactgatctg
    19501 tcgggagccg gatgcggtag ttccgcaggt ccggttctga ggaggggccc gtccgggcaa
    19561 ccggcgggtc tactcacctc atccccctac aaccactccc attcgtttaa gtatttttaa
    19621 acctgcttcc tgtggtatct ttatagataa gatcctgttt atctcaggat ttatatcgag
    19681 accttctgtc attgaaaaat cacagtgaga caaatcagaa tgtctgaact ctgtattatt
    19741 aaactccgaa ccagtaaaat cagatttacg aaggtctgtt tcctcaaata aacaatcacg
    19801 gaagcgactg gataaaaaaa tagatttctg taaccgtaaa ctaataaaat caacaaatct
    19861 caaatcacaa tttacaagag aaaaagtaca gggaaacata atgtctgctg catttactcc
    19921 ttgtaataaa caatctttga attcacagtc aataagttta aaaaaattaa gttttaaatt
    19981 ctttattgag cagtttacga agcgacattt ttcgaactca caatcgcgaa agttaaagtt
    20041 gtcaagctgt attcgttcaa atatacaatt gtaataattt acacctgata attcactttc
    20101 actgaggtgt cgttttgtga ttcttttttc tattatatcc atgtgtcagt ctcctgttcc
    20161 cataacttct ttaataaatg ccttttctgg agttgtttca ttattggatt gaataaaatc
    20221 actcatatca gaataacgat ttatactatt ttttgaaagc atatagaagg ggattttatc
    20281 tcttgttaat tcatcaaaaa tatgagttga gaaaaataca gttttcccgt cagcggtgat
    20341 cttatttatc agttcccata acatcattct gtactgaata tcaatgccta cagttggttc
    20401 atccagtaaa aataaacggg catttttgca aagcgttagc atcaaagata tgataagcca
    20461 acgcttctct ccataactta ccgtgtaagt ccttttacgt cttattttat catagcgaat
    20521 gaaaaaatta tcattccatt tatgattaag ctctgtccat gcctgttttt tagttaactt
    20581 attgaagcag caaatcaaat caagaacctc ctcattcctt agtgtacctg aaagatttat
    20641 gacttgaggt agacttacta attctccacc agagaaattt cgcttgaggg tcccttcata
    20701 attactgact ccgcccctga taaggttaaa aagagtggtc ttcccggctc cattttctcc
    20761 aagcagacct atcaactctc cctgctcaat cttaagcgac agattattga aaacaggggg
    20821 taaatttact ttatatgaaa agctcagaga gttgatttct aaaagaggta tggtcattga
    20881 gaactccaga ctggctcagt tttgaaggtt agtgctgaaa taatacttat gattaacatt
    20941 atactataga aaaatatcgt catcagagac aactcctgat ctgactggag tattgtagaa
    21001 tagatggcga taggattaac ctgatttatc cagtaaacga tcccctcatt atattttaat
    21061 gatacaattc ctgataccat acagacagtt attaatacag aatagatagt gctggctgtt
    21121 tggaatgtga ctctgaacga tgccatgaaa gtgagtgaaa ccatcataaa tgcatttatg
    21181 tatatcgatt ttaaaataat cattactatt tgataatctg gacttgcctg gaatcctgtt
    21241 agtacgacta aaatgaaaaa aaacacatac aaaatagaca tgataagtga ggctattaat
    21301 tgagaacgaa taaataatag tcgaccatcc atattatgca cgaaggtagc aataaaacca
    21361 ctttctcttc ttccgaccag atacagacag taattaaaca gaacaacaga aaaagaaatg
    21421 tagcctacaa accatgatat ttgttttccg agccatgctg tatcaccata atgaagttca
    21481 atttcattga aatgaaggaa aaagaacaac actgtaggtg acataattgt ccatattaag
    21541 ctgaaagggg ttctgatttg ttccatcaga aaaattatcg ccatttttaa tgtaaccatt
    21601 tgttatcctt atgggaatgg taccatcttt gattctctga ctttaatacc gtccccggta
    21661 tggtaaattt tattattaat gtttattgca cctgagttat tcatatgaag gaaaaaatca
    21721 gggcttacta tttttgtgta ccagacaagg ctgttactta ctctttccct agcatagtaa
    21781 acaaaaatat tatcagaaat ttgttgaaga tagtttaata aatttgtgtc tgacgtaata
    21841 ttttcctcaa gcgttaactt gacatcatca gacagtagta ccgtattttc acacaaatcc
    21901 gtaaacgact cgtacttgtt gcatgacata aagtgacgct ggtaactatc aataatgtca
    21961 tcatcagtat aaccgccaat aagaaagttg tgcaggcata tatacgattg ccacaattcc
    22021 actacggatt tacaaagtgc ttttttcaga ctattagcat aggataatcc ggtactgtat
    22081 tttattcgac tgattttgtt ttttgtgcca tacagggtta gtactgcgtg tccaggagct
    22141 ccgggcaggg tgatatcgaa aatcctgata tctccttctg acctgagagc cagtaaaatc
    22201 tcatctatat gatttatgcc tgttactatt tcactggata tttcagtatt ggcttttccc
    22261 tgtaaccagt aaagaaggag ggactgtctc tccataaatt cacacaagga accttctatg
    22321 gcatcgttca aactaccatg aaagctacat ccgcatgtat ctctgtcagg ataaaatttc
    22381 agatcatctg cagcggttat attgtcgagt gcaattatga ctgcaggtat ttcttgttgt
    22441 tcaaggctaa acagattaaa ggctctgacc gttttaaatt tatgggttat gatttcatcg
    22501 cgggtcagtg atgagatctg aaaaaatgct tcggtaaaag cctttgcagc agatggaggt
    22561 atcatctcat ataatgtttt ctgaccaccg gtgactatct cattaaaaaa atgacggcgt
    22621 tcaaaatatt cacctattgc cccctgaatt gacttcagag tttcaccggc cgcgctggct
    22681 ggtgtaatac gctcatagtc ccatatctga gaaaacgttg gcatatttct gttcagtttt
    22741 ggactcatgg ccattgtggc cggccatgcg gacataaggt tactgtagac atttatcatc
    22801 gtttacatct ccaaaaaact gaacgatcag tggcgctaca gtcctctgat ccagattcat
    22861 tgtttttgct acgtatgaat cggtatatcc cgcccatacc cggtttttca gtcctatttg
    22921 atctgcaacc aatactgcgt tctgatacat cgaacctgtt tccattaagg ccatccggta
    22981 tcctctgtag cgatatttga acaaagcttt ttcaaaaata atgcaataga cgagagcaaa
    23041 atggggttta ccaagacgtt ccgaatcccc accggacagg cttcggtaga gtgactgagt
    23101 attacatgta gcaacaggtt ccagtgcctg acttagcggc aggtagtgat aaacattagt
    23161 tcctgcctgc cagttttctg tattttcaga taatctgcat aaaaaaactt caatcgggta
    23221 caatgccccc ccagaaggat aaggtcttct tacagagcca ggatcaggtg agctgaatgc
    23281 attgactaat aacttttcaa tagtacgaat ggaaagttta tttttgtcgc aaaagtttat
    23341 tcctgatggg agcctttcac gactaaaatt atgtgatgag gatatgttta ttacggagct
    23401 gaaacctggc ctgaagagaa cttctttatt ttcaaatttc tccagatggg caagaggtac
    23461 cgcggacacg aaagttcgtt cagcaaattc ggggagggaa gcaaagttac ctctgacatg
    23521 aaaatcttta acaatggcca gaacttcagg atctgtgtaa tgcgttactt ccactaaaga
    23581 gagttcgtgt tttgacatgt tatctctcca gacagctgca agcctgccag tgaattaccg
    23641 gctctttaca taaaatacag gtgattaaat cagcgctaac agatgacata aagttatcga
    23701 catgactttt aaccagtgaa gtacctgtca actcctgaag tcgccgtttt attaaatgat
    23761 agctgaatcc tctttctgat tcgcctaaag ccaatgctgg tagtgtcatt tggtattttt
    23821 tcagtaactg aagtaagttt gcccaggaca tttcgttacg tctgaagctt ttttcttccc
    23881 tgctcagcca tctctctata tgacaaaaat ggcagggagt accaagatca ggtgaatata
    23941 ctccatctat cctgaatgac tcctttaggt agtagctttg gatgaaggca acaccagggt
    24001 tactttcaaa ttttgaatag attcttttta caatgtctga atgatagttt tcgagatgta
    24061 accagattag tgttgaatcg ctcacattaa tattattatc ttttatatca atgatatgag
    24121 aaacaaaaac accatcagat ttccattgtt cccgggcatt actgaaaaga tgtggaacat
    24181 cgcttattat gactgagttt acccagagag gtgatgtttt cgttttattt atcacgcctg
    24241 tggtaataag aaactctttg cccttgtctt tatcaataag cgattcgtcc agtagtttat
    24301 caaggaggta ttcagaaata ataccctgtt tggatcgtag gctttcgagt acttttttca
    24361 ttccctcatg tgtaatgtct actgaagaaa tgtaggtaat aagcagggtc tttgtatttc
    24421 tggaaattat ctcaaacggc agaatattga tcatatcttt tcctttttta atatcaggga
    24481 gcaccatgct ccctgaacgg ttaattcaac gtatcagata tgtgaaccac ttccgccgct
    24541 gccaccgttt ccaccactac aaccgttgct gcaaccacca caaccgccac cttgaccacc
    24601 gcagctaccg ccgccgccgc cgccgccacc accaccaatg ccaacaccta atggagactg
    24661 gcgtgataat ttaagagcat caacggacaa aactacacca aattcactcg cttttaattc
    24721 cataaggact ccttttttaa gggaaattaa tgcttatgac atctgaatag tatgttggcc
    24781 aacactagtt acttgccgta attaactata ttttgcaatg ataatttgaa ctaaatttgt
    24841 acgcctgtta aatatttgat aataatatca gttggttatg tttatactgt tattgttttt
    24901 ttacaaaaat taatattgtg tttacatttt gctttcagtt gctgtcagga tgtcacacta
    24961 cccctttaag cccccactgg tctggcaggg ccggggatgg gtaaggtgaa acctgtcaat
    25021 cagtaccggc ttacgccggt atcatatgca acaactgagt gccgccttcc atatcatatc
    25081 atttgcccgt ttcccgccgt gcagatccgg tttccggctg aagtggatcc agacctgatt
    25141 tgttgctgag tatcggtaaa agagcggctt ataatctatt ctgaaacatg gcaagtgaat
    25201 ctgggctgta taattccggc aagtgtcatc tgtatttaaa ggtcgtgtga gtatgaaatt
    25261 tatggaatgt gctgtccggg atgtgattta tgggaccaat gtccgtattg tcaaaccggt
    25321 caacatttat gaatgtgaac tcagggacaa tgtgtttgtc ggccccttcg ttgagataca
    25381 gaaaggctgt gtcattggta gtggaagccg gatacagtcg cacacgttta tttgtgagaa
    25441 cgtaacactg ggggagaact gcttcattgg tcataacgtg acgttcgcca acgatctgtt
    25501 tcgttcagga gcaccagatc cgtcaccgga taactggatc agcattaccc tgggggattc
    25561 ggttactgtt ggcagtggag ccattattct gtctccgtat atatgcagcg gagctgttat
    25621 tggtgcgggg gcagttgtag taaaaccgat tgagaacaaa ggcatttatg caggaaaccc
    25681 ggcaagactg atcagaatat tatgaaaaag aaaagggccg ttactggccc tttttatcag
    25741 aggtaggtct gtgaatacgg caggtgtaca gaaatcgact gccggggatc gatttccagg
    25801 gccgcccggt attctgcgtc agtgatatca ccttttttca gtaaataacc gagagcatcg
    25861 tttcccagcc ctgacagtat gcgtattctg gcgatccgga ctggcaaata acccatcaaa
    25921 aacgttattg ccagacattg cgaactcacc aattttgatt attggagcca ctttttcata
    25981 agttccgtga aattatttca taataacgac cctgtttatt ttatgtaatg actggcaata
    26041 aaataacttg attaagttcc ctttatccag cctgatagtg gataaaggaa actcaatcat
    26101 aatttaatta gtttattaac ttcatcgcat tcttttttgt ctcctcatca cttttttgct
    26161 ccgattgtga agaggactga atgattgttg aagaatttaa atcggccatc tcttttggaa
    26221 atagcgatct caatatattc acgatatctg aaaatgtggt ttctttcgtc agcagttcac
    26281 ataaaaggaa aggggccctg ggatcttgtc tgtataaggc cagacctgcc gtcagtgcag
    26341 cccggttaag gcggctgcgt tccccttgcg ggatactgtc cagtgtatca caaacaattt
    26401 tatctgtttg atttacatct ggatttagct taaaggcaat ggttctgcgc ttgtccatca
    26461 ttaattacct atgagataca taccgttaac taaatcatat tgagagttat tggttttaaa
    26521 aaaacgttca tcacgaatct gtgtgtgttt ttttactgca tcgcatatta attctgcgcc
    26581 accacctata accataacat gagtataacc agaaaattca ttgagtgtat ttaatacacg
    26641 ttgctcaagt ttacgaagtg cttcattcat tgcttcggtg actattgata ttttgttctc
    26701 atcattaatt cgttgcttca gatagttatt atcttttctg tgaataatta tatcatcagc
    26761 aagatagcta cttccttttg ttctcgcaag agaaagggca tcttttactg cagatgtgac
    26821 cagagataca ccaagagatg agtctccgta tattttactg atccccgata atttccccat
    26881 tacctgagaa atatctaatg tggtgccccc gagatctata attaataaag aatctaactc
    26941 atccagttct tgtagaactt cataacctgc cggtatagat tcaggcatga cttttacatc
    27001 ttttattgtg aatgtatccc caccatttaa tgtaattttt ttccggaagt ttgctttctt
    27061 acgctcaata ttttccgtat tgggttggtt attcctgtcg taatactctg tcagaggaag
    27121 tgtgcaaaca atatccactt cgcttaccgg cagaccactg gtcagtaagg cgtggtgcac
    27181 cgcaacgaca ttaacgtcgc tgtattgcca tgcgatattg gttgtgacta cagcatccgg
    27241 gctgattgga tcaaatgaat actgttcgcc gttcagtgtg tagttaaaga cctttttatc
    27301 accaaaagaa actgcccact cgcgtttgaa gctgttcggg ctaatgtgct gtttaattgt
    27361 tccgtcgctt tcctgccact gtagtttgat gtttgttgaa ccgtcatcaa tgaataccaa
    27421 catttataaa actccttatg gtgttttttt ggtgtttttt tgggtgtgtt ttgggcttta
    27481 aatgggttta tttgtcaagt ttaccccatt ttaactatca atcaatgatt atttgtcttg
    27541 ttttggtgtt ttgttgggct tggtatgggt tgtttttggg ttttgtttgg cggataacaa
    27601 aagatgtcgc cgggctcctc tttttactgc gccggctgac gcggcgcggc aggaacgctg
    27661 cctgtggtca gtgtcccgcg tccggccggg cacgggacgg gcgcttttac cgctcccggc
    27721 tggcgtcggt gacagtgtac gccagcccgt cgcccttttc tgatgagcgc ccttcaagcc
    27781 gctttcgcgg catagccttg ccgtcaaaaa gacgacggct gcggtattcc acggtcggcc
    27841 tgacccgtta ccgggcgcgg tgaacagccc acaggcagcg gggaacgggc gccacagggg
    27901 cgccctcccg atgctcctgt gaggataatt cccatgccaa actggtgctc gaatcgtatg
    27961 tatttttctg gtgaaccggc acagatcgct gagattaaac gactggccag cggtgcagtc
    28021 acaccgcttt atcgccgcgc cacaaatgaa ggtattcagc tgtttctggc cggaagtgcc
    28081 ggacttctgc agatcactga aaatatccgc tctgagcagt gccccggagt gacggctgcc
    28141 ggacgtggtg ctgtatcact ggaaaatatc gcgttcaccc gctggctgac acacctgcag
    28201 aacggtgtac tgctggatga gcaaaactgc ctgatgctgc atgaactctg gctgcagagc
    28261 ggtactggac agcgtcgctg ggaagggtta ccggatgatg tcagggaaac tatcactgtg
    28321 catttcaccg caaaaagagg tgactggtgt gacatctggg gtaacgagga tgtatcggtg
    28381 tggtggaacc gtctgtgtga caacgtactg ccggaaaaaa ccatgccgtt tgacctgctg
    28441 acggttttgc cgacccgcct ggatattgaa gtgaatggct ttaacggcgg tgttctgaac
    28501 ggtgttcctt ctgcatatca ctggtataca gaacggtatg gcgtgaagtg gccttgtggg
    28561 tatgacctga atatcagcag tcagggggaa aacttcattc aggtggattt cgacacgccg
    28621 tggtgtcagc cggaaagcga cgttgttgca gaattaagcc gccgtttcgg ctgcacgctg
    28681 gagcactggt acgccgaaca gggctgcaat ttctgtggct ggcagttgta tgagcgcgga
    28741 gagcttgttg atgtgctgtg gggggaactg gaatggtctt ccccgacaga tgacgatgag
    28801 ctgccggaag tcaccggacc tgcgtggata gtcgacaatg tgacgcatta tggcggatga
    28861 agtatgacgg agacgggcgg gcaaccgccc gtttcttttc cgacaaagga tgtcgccgtg
    28921 ctcctctttt tactgcgccg gctgacgcgg cgcggcataa acgctgcctg tggtcagtgt
    28981 cccgcgtccg gcgggcacgg ggcgggcgct tttaccgctc ccggctggcg tcggtgaaag
    29041 tgtacgccag cccgtcgccc tttactgatg aacgcccttc aagccgcttt cgcggcatag
    29101 ccttgccgtc aaaaagacga cggctgcggt attccacggt cggcctgacc cgttaccgga
    29161 cgcggtgaac agcccacagg cagcggggaa cgggcaccac aggggtgccc tcccggtgtc
    29221 ctctgtaaga gaaggagtgc tgttatgtcc cgttttatcc tgggtgactg cgtgcgtgtt
    29281 atggccacgt ttccgggcaa tgctgtcgat ttcatcctca ccgacccgcc gtatctcgtc
    29341 ggtttccgtg accgttccgg gcgcaccatc gccggcgata aaaccgatga atggctgcaa
    29401 ccggcctgta atgaaatgta ccgcgtgctg aaaaaagatg cgctgatggt cagcttctac
    29461 ggctggaacc gcgtcgatcg ctttatggcc gcctggaaaa atgcgggatt cagcgtggtt
    29521 ggccacctgg tgtttaccaa aacctacaca tcgaaggccg catatgtggg ctatcgccac
    29581 gaatgcgcct acatcctggc aaaaggacgt ccggcgctgc ctcagaagcc attgcccgac
    29641 gtgctgggct ggaaatattc aggcaatcgt catcacccga ctgaaaagcc cgttaccagc
    29701 ctgcaaccgc tcattgagag cttcacacac ccgaacgcga ttgtgctgga cccgtttgca
    29761 ggcagcggct caacctgtgt ggccgccctc cagtccggac gccggtatat cggtgtcgag
    29821 ctgcttgaac agtatcaccg tgccggacag caacgactgg ccgccgtgca acgggccatg
    29881 cagcaggggg ccgcgaatga taactggttt gaaccggagg ctgcgtaaat gaactatgcc
    29941 ggacacgaaa aacttcgcgc cgacgtggcg gaggtggcca acaccatgtg tgaccttcgg
    30001 gccaggctga acgatatgga gcatcgctgt cgctttgatt ccgatgtgct tgttgaacgc
    30061 ctgacacgcc agacgctgta tcgcgccaat cgcctgttca tggaggcgta taccgaaatt
    30121 ctggaactgg attcctgctt caaagattga gaaggagacg agaatgtacg gaacatgcga
    30181 aacgctatgc cgggagctgg cagcaaagta tccgggagac acgccgctga tgctggttat
    30241 ctggtccccg gaagaaattc aggccctcgc tgacggaatg gatatttccc tgtccgatca
    30301 tgaaatcagg actgtcctgg cgcgcctgga ggacatcccg gaagaccagc ggactgaatc
    30361 cggtatttct tccggtgcgg cgatggagat catcaataac gtgagagaaa accgccaggt
    30421 aaccgtccct gctgaactgc tggcgtccct gattcagacc gctgaacagg cattgtggaa
    30481 acgtgaatgg gccgcccggg atcatggcct cgccgtcccg gaatgcgtca cccgccgtca
    30541 ggcggtggtt aatcaggtcc gcatcctgct gaaaaacaac acacacgaaa acgactgatg
    30601 ttatcgccgc ctccgggcgg cgatacaggg agttcatgaa ttatgaatga aacgctgaat
    30661 gcactgattt atcgccacgc cagcaacctg ttgctggcgc agggctggcc ggaagagacg
    30721 gatgttgacc agcgtaaccc gaagtatccg ggctggatca gcatttacgt gcggctggat
    30781 gcgccccggc tggcgacgtt acttatcaac cgtcacggcg gcgtactgcc gccgctcctg
    30841 gcctctgcca ttcagaaact gaccggaacc ggagcggaac ttgttctgtc cggcagtcag
    30901 tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc gtatgccggt
    30961 gagtggctgg cagaggacga aatcagggcc gttcttggtg cggtacgcga tgcagtatgc
    31021 agtgtcagtt accaggtggc agaagatgcg cggcgtatcc gtgcggcgct gaccaccacc
    31081 ggtcagacgt tgctgacccg ccagacgcgc cgctttcgcc tggtggtgaa ggaaagcgat
    31141 cacccctgct ggctcgatga agacgacgaa aacctgcccg tggtgctcga tgccatcctg
    31201 aaccggggcg ctcgtttttc gtcggtggaa atgtatctgg tcagtgagtg tattgagcat
    31261 atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac cggatgaacc gccccgccgc
    31321 tggtttgacc gtggtgttct gcgggaggtg gtccgggaag cccggaccga aatccgcagc
    31381 atggcggatg ccctggcaaa aatccggaaa tgatgacact ggcggggaaa aatctccgcc
    31441 ttttccagcc ggtccccgga aagagcaaaa ggatgtcgcc gcaggctccg ctttacccgg
    31501 ccatgcgggg catggccttg tgggttttca gttatgtggc ctcagcgtcg tgtgcgggct
    31561 gtgccgtgcc tccatcgtag ccgggcttct gcgggcgggt tgcaaggtaa ggtgaaccgc
    31621 tgcggtcacc cggtccctcc ttcccgtctg ccgtgatttt ccggtcgttc acccgctcag
    31681 atttcgcggt ctcaccctcc aactcccgtc agccgtgcgc gtcaggctgc ggcttccctt
    31741 gcgcccttgc aaccctgcag gcccggcaaa gatggaggca cggcaccgcc cggtgtcgcg
    31801 gaatatgtga acaacggagg aaaacatcat gcaatatgcg aaacctgtca ctctgaatgt
    31861 tgaagagtgc gaccgtctct cctttctgcc ttacctgttt ggcctggatt ttctgtatgc
    31921 cgaagcgtct gtatacgcgc tggcgaaaaa aatgatgccg gaatatgaag gcggattctg
    31981 gcacttcatc cgcctgccgg acggtggcgg ttacatgatg ccggatggcg accgtttcca
    32041 catggtgaac ggtgcaaact ggtttgaccg tacggtcagc gccgatgccg caggcatcat
    32101 tctgacttcc cttgtgatta accgccagtt gtggctgtac cacgacagcg gggatgcagg
    32161 actgacccat ctttaccgga tgcgcgatgc gcagttgtgg cgtcacatcg aatttcaccc
    32221 cgaatgcaac gcgatttacg cggcactgga ctgattaacg gacggggcgg caatgccgcc
    32281 cctgtaaagg agacgagaac atgtactgta ctgttaaaga aatcatccgc gatgtactgg
    32341 atacagacgt gccggacagt gaatgcgttt ttgccgtggt gctgacccgt ggggatgtgc
    32401 gctacatagc ccaggactgg aacctgtcag acgatgagct ggaaaccgtc atgcagcggc
    32461 tggacgatgc ctttgaatat ggtgcggatg tcagcattgt tcacgacgtt gttcgtgagc
    32521 tgatggaaga aaagcgagcc agccgtcagg tgacagttcc ggcggtgatg ctggaaaaag
    32581 tgatggcgct ggcaggcagt gaaatgaagc gcctgtatgc cgtcgggagt gagaacgggg
    32641 gcgacggtga cgcattcgtc agggaagaac gcgaagcaat ggacgttgtg ttacaggcgc
    32701 tggacgggga gcacatgtca tgaatatcag cacagaaacc cgcgaaattc tgcgtaatta
    32761 cagggccgtg attaatgcgc ggcgtcgtga aatggggcag aaaccgctca ccaccgcgca
    32821 gattgttgat gaaatctgcg attttgtggc gaatcagcag gcggttttcc tgggcggtca
    32881 ctacatctta cagggtagca gaaacaggtg atacaggggc aggcggtgga ggccgcctgt
    32941 cagtccgcgc gaaaacccct ggcaggctga cgcctgccgg aagggaagcg ggccacgctg
    33001 gcgcgtcgcc cgttaccctt cccgtctgtg atgtgtctga ctcaggagtg tttctttctt
    33061 atgttatgga actgttcctg atgttgttgt tcctttatct gctgctctga ttctgactac
    33121 tgcagtgttg ttctgtctgc cgggcgcacc ttcggctccc ggctgcctgg cggcatccgg
    33181 tcgtcaggcc cgactccgac aggcagctgc tggcgcaccc gccagtctcc gccgaacccg
    33241 ctggcgcggt ttcgggctgt cgctacggtc cgatgcgtga aaaccccggg cttgcagcga
    33301 ccgttccggt cgcgccaccc ttgtcgcgcc gtggcctgcg ctcccggcat cccggcaaaa
    33361 ggccgccggg atgtaagggg caacgccgtt tcgcgcacgc gctctccgtc gttgcggcgg
    33421 gtccctgctc attgcctgcc ggtgccacac acccgtatga gtccctgtcg cctggggctg
    33481 atacagccac aacctgccgc actgactgtc cagggtaaat gaacagccca caaaattttt
    33541 tacccaaaac ccggtaaaaa attttgcgtt ctgcccagga caggtgcgtc aggccgtggc
    33601 agtgatgccc cttgcggcga aggttctcaa acgggtgaag ttcataccac agggtataca
    33661 gcagcaggtt cccgcgaaac gtcaaaaccg cggctcccga aactcaacgg gtgatgttct
    33721 ttaaaaatca ggatttattt caggctccgg caatacgcat ttatgtgttc tgtcagtcat
    33781 ccggctggca ggtaatgaga acgcaggaac agaaggagga aaacaccgga gaacgtcaga
    33841 cgcagatatg aaaaaagcag gtcagggacc tgcttaatcc acaccgcaga gactgcacga
    33901 acagtcttta cggtttcatt aattaacgtc ataagaggct aaattatgac agtaagtatt
    33961 gtttcacctt ccgcagcggc tgtaaagccc cgccgtcatc cccgtttcag acgcgaagac
    34021 atccccgcgc cggaaattga ccccgtgctg aaagccttcg ggcgacacat tgcccggagt
    34081 tttcaccggg ggcgtggtgt acacatcccc gccatgaaaa acacggcctt cgggcaggtg
    34141 ctccgcacac tcgaactgaa acgcgctttt aactgatgag gtgagccgcc ccgcgagggg
    34201 cggcaacaga cggaaggaga acattttatg acattagcag caatcaccat gaccgccccg
    34261 gaagccgcca gccctgtgca gatgtaccgc gcgacctact caccggatga caacaaactg
    34321 cgcctgtatg ccgtgtcacg tcttgacccg gagacgtata aaaaagtgca tgatgccggt
    34381 ttccgctggg caccaaaaca ggcgctgttt gtcgcgccag cctggacacc gggccgggaa
    34441 gacgtgctcc tctcacttgc cggagagatt gaggatgaag acagcacgct cgctgaacgt
    34501 caggaagcac gggcggagcg gtttaccgga tacagcggaa agcgggccag tgaatccgca
    34561 caggcacttg atgaagtgga aagactggcc gcgatgatcc cgcccggtca gcccattctt
    34621 gtggggcatc acagcgaacg ccgcgcccgt cgtgatgcgc agcgtattga aaacggcatg
    34681 aaacgagccg tgatgctctt tgaacgtgcg gaatactggg aagagcgggc gcggtcggca
    34741 ctgcttcacg cgaagtataa agaacgtccg gacgttcgct ggcgtcgtat caaaaaaatc
    34801 gaagctgatt tgcgcaaggc tgaaaagacc atcgcgcagt cgcagaaata tctgacgatg
    34861 tggcgggctg aatcgctgga tctgaatatg gcaaaactca tcagcagtca tgaccatatc
    34921 agcgcctgtt tcccgctgga tacgtatccg cgcccggcag aaaaaagcca gtatgaagga
    34981 agtcgatcgt tatggtcggc cctggatgat gacatcatca ccacggagca ggcccgcgaa
    35041 attgcgatcc gctgtcatga acggcagatt cagcatcaac aacgctgggt taaccactat
    35101 cagaaccgcc tgatctatga gcgtgccatg ctggacgaaa gcggcggcgt ggttacccgg
    35161 acacaggatt ttgagccggg cggacaggtt ttcagccggg gcgagtggct gaccatcatc
    35221 cgtgtgaaca aaagcaacgg ggcggtgagt tcagtcacaa cgccaaatta cagttttctc
    35281 gggtacagcg gcacgatgaa agtgacgccc gatcgcatca cggactacaa agcaccatcg
    35341 gcagaagagg ctgccgtcgc cagtcaggcc gcgaagcgtc cgccggtagt caactatccg
    35401 ggggaaggtt tccgggaaat gacaaaggca cagtgggccg ccctgccccg ggactgtaag
    35461 gccgtgcgca gtgtggcaga agcagaagac cacggggcat accggtaccg ccgcacaatg
    35521 gacaataatt tccgcctggt gaatgtgtat atctccgaca tgaaaatcac ggaaatccca
    35581 cagaaataag gtatatcccc ccgggaaaat ccccggggga tgacggagat aatgatatgc
    35641 acagtcagtt aagagaacgt atccggctga tgcgcgcaag gctggataac gccgcgccgg
    35701 ttgctgaaat ccgggctgaa tctcagcttt ttgtgactcc tgccccggtc tgtgatcgcc
    35761 tggtgatgct ggcggagatc agcaaccgtg atcacattct ggaaccctct gccggcaccg
    35821 gggccatact gcgggcgatt cgggataccg cgccgggggc catgtgtgat gcggtggaaa
    35881 tcaacagcgg gctggtccgg tatctgcggg aaaattttaa cggtgtcagg gttcagtgtg
    35941 gcgacttcat ggaatggctg ccggtgcagt attacagccg ggttatcatg aatccgccgt
    36001 tcagccacgg gcaggatatc cggcatatcc tgcgggcctt ttccctgttg cgtccgggtg
    36061 gcgtgctggt cgccgtctgt ctcaacggac cacgccagca ggagaagctg ttaccgtttt
    36121 ctgacgtccg cgaggagctg ccgcgcggca cgtttgctta taccgatgtc ccgacgatga
    36181 ttattcgtct gcgtgcctga tgacacaggg ccggcgggag gataaatgcc tgtctgcccg
    36241 cgccgggaaa aaacgcgcgc cggtggcgct gaaaggggcc gcgctcacgc gccgcccctt
    36301 tcattacgcg gacgttctga tgggggcttc tgtttcttca tgcctgtcat ttgtctgtta
    36361 ctgatgttgt tgttcctgct actgctactg ctactgctac tgctactgct actgctactg
    36421 ctactgctac tgctactgtt cttctttttc ctgctgctct gattctgact actgcagtgt
    36481 tgtcctgtct gccgggcgca ccttcggctc ccggctgcct ggcggcatcc ggtcgtcagg
    36541 cccgactccg acaggcagct gctggcgcac ctgccagtct ccgccgaacc cgctggcgcg
    36601 gtttcgggct gtcgctacgg tccgatgcgt gaaaacccct ggcttgcagc gaccgttccg
    36661 gtcgcgccgc ctctgtcgcg ccgtggcctg cgctcctggc atcccggcaa aagaccgccg
    36721 ggatgtaagg ggcaacgccg tttcgcaaac cgcgcttcac gtcgttgcgg cgggtccctg
    36781 cccgttgttc tgtccggtgc cattcacctg tttaagcccc tgtcgccggg ggcaaagaca
    36841 gccacaaccg tctccgccag tccaggggtg aaatgcacat cccgcaaaac tttttgccca
    36901 gaagcaggca aaaacttttg cggtctgccc ccggacagtc gtttccgtcc gtggcagtga
    36961 tgccccatgc ggcgaaggtt ctcaaaaagg tgatggtgaa taccggaggg aacaaaaggc
    37021 aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aaaagatgat gggggctgaa
    37081 aacatcaaca gaaggaggca catcatggca gttcgtggca ttaacaaggt cattctcgta
    37141 ggacgcctgg gaaaagatcc ggaagtccgt tacatcccca acgggggcgc agtggcaaac
    37201 ctgcaggtcg ccacgtcgga aacctggcgt gacaaacaga caggggagat gcgggagcag
    37261 acagaatggc atcgcgtggt gctgttcggc aagctcgcgg aagtggctgg cgaatatctg
    37321 cgcaagggcg cgcaggtcta catcgaaggt cagcttcgca cccgtagctg ggaagataac
    37381 ggtatcaccc gttacgtcac cgaaattctt gttaagacca caggcaccat gcagatgctg
    37441 ggacgtgccg caggtgctca gactcagccg gaagaggggc aacagttcag cggtcagcct
    37501 cagccggaac cacagtcgga ggccggtacg aaaaaaggtg gcgcaaaaac gaaaggccgt
    37561 ggacgtaagg ccgcgcagcc cgagcctcag ccgcaaccgc cggaggggga ggattacggg
    37621 ttttcagacg atatcccgtt ctgatcgggc tgactgtgat aaccgccccg ccccgttctg
    37681 tgcggggcat caccggagag atgagaatga gcgaatattt cagaatactt caggggctgc
    37741 ctgacggtcc ttttacccgc aaacatgccg aagctgttgc cgcacagtac cggaacgtct
    37801 ttatcgagga tgaccacggc gaacagtttc gcctggttgt ccgtaataac ggtgcaatgg
    37861 tctggcgtac ctggaatttt gaggacggtg ccgggtactg gatgaaccat gtcatcaggg
    37921 atttcgggat tcttaagtaa gagaggtgcc ggacggggac acgtccggca gacaatcatt
    37981 attaccgttt tgggaggtac taactatgtc agttacagag tctaaggcaa aaacggagcg
    38041 taaatccagc cgtaaacctg caaaaacgca ggaaacagtc ctgtctgccc tgctggcgca
    38101 gacggaggaa gtgagcgtgc cgctggcctc actgattaaa tcaccgctga atgtgcgcac
    38161 ggtgccgtat tctgtggagt ccgtcagcga actggctgag tccattaagg gcgttggcct
    38221 gttgcagaat ctggtcgtgc ataccctgcc tggtgaccgt tacggtgtcg ccgcaggtgg
    38281 tcgccgactg gctgcactca acatgctggc agagcgcaac atccttccgg ctgactggcc
    38341 tgtacgcgtg aaggttattc tgcaggaact ggcgactgcc gcatcaatga ctgaaaacgg
    38401 tcatcgtcgg gatatgcacc ctgccgaaca gattgccgga ttccgcgcaa tggcgcagga
    38461 aggcaaaaca cctgcgcaga ctggcgacct gctgggctat tcaccccgcc acgtccagcg
    38521 aatgctgaaa ctggctgacc ttgcgcctgt catcctcgat gcgctggcag aagaccgcat
    38581 caccaccgaa cactgtcagg cgctggcgct ggagaacgac accgcgcgtc aggtgcaggt
    38641 gtttgaagcc gcctgccagt caggatgggg cggtaaaccg gatgtgcggg ttatccgcaa
    38701 cctgattacc gaaagtgaag tggcggtggc tgggaacagt aaattccgct tcgtgggggc
    38761 tgatgccttc tcgccagacg aactgcgcac cgatttgttc agcgacgacg agggtggcta
    38821 tgtggactgc gtggcgctcg atgccgccct gctggaaaaa ctccaggctg tcgcagagtt
    38881 ccttcgggaa gccgaaggct ggggatggtg tgccggacgc atggagcctg taggtgagtg
    38941 ccgtgaggat gccggaacat accgctgtct gccggagccg gaagcggtgc tgacggaggc
    39001 agaagaagaa cgcctgaacg aactgatgac gcgttacgac gcgctggaaa accagtgtga
    39061 ggaatccgac ctgctggaag cagaaatgaa gctgattgac tgcatggcga aggtcagggc
    39121 gtggacgccg gagatgcgtg ccggaagtgg cgtggtggtg tcctggcgtt atggaaacgt
    39181 atgtgtccag cgtggtgtgc agttgcgcag tgaagatgac gtgactgatg acgctgaccg
    39241 cacggaacag gtgcaggaga aagcgtcagt ggaggaaatc agtctgccgt tgctgacgaa
    39301 aatgtcttcc gaacgcacgc tggcagtcca ggcagcactg atgcagcaac cggacaaatc
    39361 cctgacactg ctggcatgga cgctctgcct gaatgtgttt gacagcggag cgtacagtaa
    39421 accagcacaa atcagcctgg aatgtaaaca ttattcgctg accagcgatg cgccgtcagg
    39481 gaaggaaggt gccgcattcc tggcgctgat ggcagaaaaa gcccgtcttg ctgccctgtt
    39541 accggaggga tggtcacggg acatgacgac attcctgtca ctcagtcagg aggtgctgtt
    39601 atccctgctc agtttctgca ccgcatgcag ccttaacggt gtccagaccc gtgagtgtgg
    39661 tcacacgtca cgcagtccgc ttgacccgct ggaaaccgct atcggattcc acatgcgcga
    39721 ctggtggcag ccgacgaaag caaacttctt cggacacctg aaaaagccgc agattatcgc
    39781 agccctgaat gaggcaggac tgtccggtgc cgcacgggac gcggagaaga tgaagaaagg
    39841 cgatgcggct gaacatgcag agttccatat gaaagacaac cgctgggtgc ctggctggat
    39901 gtatgcacca cgtccacaga cagataccac tgaacgcacc gataacctgg ctgatgccgc
    39961 ctgatgaaca accacaccgc cccgccggag acggggcggc agcaagggag ataccgtgat
    40021 gaaaactgaa ctgaccctga atgtattaca gaccatgagc gcacaggaat atgaagatat
    40081 ccgggcagcc ggaagtgatg aacgtcgtga gctgacacac gccgtgatgc gggaactgga
    40141 tgcgccggac aactggatga tgaatggcga gtatggcagt gagttcgggg gctttttccc
    40201 cgtccaggtt cgtttcacgc cagcccacga acgtttccac ctggcgttat gttcaccggg
    40261 cgatgtctct caggtctggg tgctggttct ggtgaatgcc ggtggtgagc cgtttgcggt
    40321 ggttcaggta cagcggcggt ttgcaccgga agccgtcagc cattcactgg cactggcggc
    40381 gtcactggac gcgcaggggt acagcgtcag tgacatcatc catatcctga tggcggaagg
    40441 aggtcaggca tgagcgcacg ttcacaggca ctggttcctc tcagcacaga gcaacaggcc
    40501 gcatggcggg cggtggcgga gaccgaaaaa cgccgccatc agggtaacac gctggctgaa
    40561 tatccgtatg cgggggcgtt tttccgctgt ctgaacggaa gccgcaggat atcgctgtct
    40621 gacctgcgat ttttcatgcc gtccctgacc gcagaagaac tgcacggcaa ccgtcttcag
    40681 tggctgtatg ccatcgatgt gctgattgaa acacagggcg aggtctgcct ccttcccctt
    40741 cccggtgatg cagcagagcg cttgttcccg tcggtgcggt ttcgtgtcag ggaacgcagt
    40801 cgccataaat ccgcgctggt catgcagaag tacagccgcc agcaggcgag ggaggcagag
    40861 cagaaagccc gtgcgtacca ggcgctggtc gctcaggctg aaatcgaact ggcattccat
    40921 tcgccggaaa cagtcggaag ctggcatgca cgctggagtg accgggtggc agagcatgac
    40981 ctggaaaccc tgttctggca gtggggcgag cgttttccgt cactggccgg aatggaacgc
    41041 tggcagtggc aggatatgcc attctggcag gtcatcgcag aggctggtat ggcggcaagg
    41101 gtggtcggtc atgctgttcg tgagatggag cggtggatgg tgccgaacaa actccgggag
    41161 gcagcgtgat gcggcaacaa tcacacggat ttcccgtgaa cggtctgaat gagcggatta
    41221 ttttcaggga aagtgagtgt ggtcagcgtg caggtatatg ggctatgatg tgcccggcgc
    41281 ttgaggcttt ctgcctcatg acgtgaaggt ggtttgttgc cgtgttgtgt ggcagaaaga
    41341 agatagcccc gtagtaagtt aattttcatt aaccaccacg aggcatccct atgtctagtc
    41401 cacatcagga tagcctctta ccgcgctttg cgcaaggaga agaaggccat gaaactacca
    41461 cgaagttccc ttgtctggtg tgtgttgatc gtgtgtctca cactgttgat attcacttat
    41521 ctgacacgaa aatcgctgtg cgagattcgt tacagagacg gacacaggga ggtggcggct
    41581 ttcatggctt acgaatccgg taagtagcaa cctggaggcg ggcgcaggcc cgccttttca
    41641 ggactgatgc tggtctgact actgaagcgc ctttataaag gggctgctgg ttcgccggta
    41701 gcccctttct ccttgctgat gttgtacggg catgaacaac ctgacttcag gaaggtcgtt
    41761 tcccttcagg aacggggatg aacgcgcgcc tgcggcgccc gttcttttcc cccgccttct
    41821 ctggttatgg cctgtcagaa atcacatcac cttccctgtg attattctct ttttcttcct
    41881 gctctgattc tgactactgc agtgttgtcc tgtctgccgg gcgcaccttc ggctcccggc
    41941 tgcctggcgg catccggtcg tcaggcccga ctccgacagg cagctgctgg cgcacctgcc
    42001 agtctccgcc gaacccgctg gcgcggtttc gggctgtcgc tacggtccga tgcgtgaaaa
    42061 ccccgggctt gcagcgaccg ttccggtcgc gccgcctctg tcgcgccgtg gcctgcgctc
    42121 ctggcatccc ggcaaaagac cgccgggatg taaggggcaa cgccgtttcg caaaccgcgc
    42181 ttcacgtcgt tgcggcgggt ccctgcccgt tgttctgtcc ggtgccattc acctgtttaa
    42241 gcccctgtcg ccgggggcaa agacagccac aaccgtctcc gccagtccag gggtgaaatg
    42301 tacatcccgc aaaacttttt gcccagaagc aggcaaaaac ttttgcggtc tgcccccgga
    42361 cagtcgtttc cgtccgtggc agtgatgccc cttgcggcga aggttctcaa aaaggtgatg
    42421 gtgaataccg gagggaacaa aaggcaggtc cccgcaaaac gtcaaaaccg cggctcccga
    42481 aactcaacag atgatggggg ctgaaaacca gagagaggag aaagcaggat gagtacccga
    42541 aatatccacg ttaacaccgc gtcgtacacg cttcttgttg ccgggaagaa aaaaaacacc
    42601 ggggaagagt gggacgtcct ggaattcagc agcctgactg agctgaaaaa atatcgcaaa
    42661 agccacccgg aaaagatggc cttcagttat agctacgcgc tcagtcaggg tgtggataag
    42721 cagttccgcc atatcaacat tgcggaagcc gatcacttca aacagttcct gcgtcagatt
    42781 aagcgtgccg gcctcgatat ccgggcgatc tgctgaccgg atgtgatgaa tacccctcct
    42841 gattgttgca atcttgttaa tggagatcgg gggggatgat attaagcgga aaaatttttt
    42901 attaaattta ttctttcata aaggagtagc tgttatgcga ttagcttccc gttttggtcg
    42961 gtataattcc atccgccgtg aacgtccttt aacggatgat gaattaatgc agttcgtgcc
    43021 ttcggtattt tccggtgata aacatgagtc ccggagtgaa cgttatacgt atattccaac
    43081 aatcaatatc atcaataagt tacgtgatga aggtttccag ccattctttg cctgtcagag
    43141 tcgggttcgt gatttgggac gtcgcgaata cagtaaacat atgttacgtc ttcgcaggga
    43201 agggcatatt aacggacagg aagttcctga aattatcctg cttaattcac atgatggttc
    43261 atccagttat cagatgatcc ccggaatttt tcgttttgtc tgcacaaatg gcctggtgtg
    43321 cggaaataat tttggcgaaa tccgcgttcc acataaaggt gatattgtcg ggcaggttat
    43381 cgagggagcg tatgaagtgc tcggtgtctt tgataaggtc actgataata tggaggcgat
    43441 gaaagaaatt catcttaaca gtgacgagca acatttattt ggcagagctg cactgatggt
    43501 caggtatgaa gatgaaaata aaacgccagt gacacctgaa cagataatta ctccccgtcg
    43561 tcgggaagac aaacagaacg atctctggac aacctggcag cgggttcagg agaatatgat
    43621 aaaaggtgga ttatcggggc gaagtgcctc cgggaaaaat accaggacaa gagccattac
    43681 aggtattgat ggtgatataa gaatcaacaa ggcgttatgg gtgattgccg aacagttcag
    43741 aaagtggaag tcatgataat ataagtaaag ccccgaaaaa attttcgggg ctttacttat
    43801 attatcattt ttcggcattg ctgtgtcgtc tgatatacct caatttcatc aaccggcttt
    43861 ccggttgtct gtaaatattg tgcctgcgaa aaatggtctg gcaggaggct gcgcagttac
    43921 cgggtatata taagatgggt tcccgcttcg cggggcttcg gccagtcagt attgtcactc
    43981 atatgatatt tttgtgtgtg gccttccatg ccgcttttgc ggcataaccg gtatctgaca
    44041 atgtctgtaa gattaattgt tctgcacgct gttaatttcc ggaagtgatt tcttcgtggc
    44101 cggaagccgg atacctttac tgctttttat cccggtataa atccggtaaa catctgatgc
    44161 gtaggcaaga cgtctctggt tctggcgttc ggttttcctg aatccggcat tgtatgcacc
    44221 aacggcctcc caggtgacgc cccatttttt aaatgctatt gccagataat aagcaccggt
    44281 ataaatgttc atgcagggat ctgttgtcag atgttctggc ttaattccgt agcgggccag
    44341 ttcgttaaaa tgctgggaat ctacctgcat cagtccgctg ccatatcccg ttaccggatt
    44401 aataccgatg gcattaaccc ggtaacggga ttcttgccat gatattgctc tcagtaaatc
    44461 aggatctatt ttgtaatccc ggcctgcaag atcaaagcaa tcagtggctt ggcagatctc
    44521 atttataaac atcaggcaga tggctaacat ccattttttc atttttccac ctctggtgac
    44581 tttatccgta aataatttaa cccactccac aaaaaggctc aacaggttgg tggttctcac
    44641 caccaaaagc accacacact acgcaaaaac aagtttttgc tgatttgcta cttgaatcat
    44701 taacttatgt tttaaataat gtattttaat ttattttaca ttataaaaag gattcattgg
    44761 tgaatcgcat atgattcacc aatgaattta acacaactca tagatgaatc agtaataata
    44821 aaacaacgag ttaaagcgaa ttgactctag attcaattcg tgatgtgatt tgctgcatga
    44881 atcgtaattt tcgtaacttg cgtcacgatt cgattcatcg atgaattgtc ttttttttat
    44941 atgtgaaata aatttgatgc gataagaaaa taaagaggta tacatatggc gaaagtgcag
    45001 gcttatgtca gtgacgaaat tgtctataaa ataaataaaa ttgtggaaag aagacgtgca
    45061 gaaggagcta aaagtacaga tgtaagtttc tcaagcatat ccactatgct tctcgaatta
    45121 gggcttcgtg tatatgagac tcagatggag cgtaaagagt ctgcatttaa tcagactgag
    45181 tttaataaat tgcttcttga atgcgttgta aaaacacaat catcagtagc gaaaattttg
    45241 ggtattgagt ctctcagtcc tcatgtctcc ggaaatccaa agtttgaata tgcgaatatg
    45301 gtcgaagata tcagggagaa ggtgtcatct gagatggaga ggttctttcc agaaaatgat
    45361 gagggttaaa aaagaattaa aagttgattt gcttcaaata acaggatttt gatctggttc
    45421 aatttgatga caattaaccg cataaaggtt atattaatta tgtggttaat gccacgttaa
    45481 aacttgatat taaaaatcgc cgatgagaga aaggccactt aacagtcaat ctgtaaataa
    45541 atacattctt aacgttcaaa atatctacag gaattctcct gttcctgttt gtgtccgtaa
    45601 caaaaaaagg aaaatccttt atgccaatgg ggcctttatt gaactctttt ccaaagaaga
    45661 taaacccttc tctggtgaaa gttatgtacg tctgcaggtt gaaatttttc tttcatcact
    45721 tgagcttgaa tgccagtctc ttgggcatgg ttccgcattt tgtcgtcgtt ttaattttca
    45781 tggagaaatc tatcaaataa ggatggaaaa tgtttcattt tataatgaag aatctgttgt
    45841 tctatggcaa attaatatat ttcctgacta tccattcttt cgtgtggaaa aagaaaatta
    45901 ttatcatcgt gatagctatg tacagtctgt tatatcgaat atgacagcaa aatcattggt
    45961 tgttttttgt ttctatgcat tagggtataa acatataaat atagctaaag aattaaaaat
    46021 cacagaagtg gcttctaaaa aaaggattaa aaagattaat gcggatatca ataaaagatt
    46081 gggttccttc gatttgttca ggaataaatg tattaagaca ggtgtaatgt acgaaatatt
    46141 aaatgttttg agtgaatata tgggtgttaa aaaaatgtaa taaagcaggg ctcgttttgg
    46201 gcccattttc gacaggccat ttctgtgcaa tcatgatttt attggtcagg aaacagaggt
    46261 gaattgagtg aggaggcgta acgcgagagg cgggataagc agaacagtct cagtgtatct
    46321 cgatgaagat acaaataacc gacttattcg ggccaaagat cgtagcggta gaagtaggta
    46381 atggtgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc cgtggcttcc
    46441 atctccatca gttgtccctc ctgctcagct actgaaggcg tggtgcgtaa cggtaaaagt
    46501 actgccggac atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc
    46561 acttacaccg cttctcaacc cggtacacac cagaaaatca ttgatatggc catgaatggt
    46621 gttggatgcc gggcaaccgc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac
    46681 ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt gatgtgattg
    46741 tctgcgctga aatggacgaa ca
//
LOCUS       DAEBXD010000024        46118 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEBXD010000024 DAEBXD010000000
VERSION     DAEBXD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.024"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(19..606)
                     /gene="mog"
                     /locus_tag="KJL94_001344"
     CDS             complement(19..606)
                     /gene="mog"
                     /locus_tag="KJL94_001344"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBD4409765.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(721..1674)
                     /gene="tal"
                     /locus_tag="KJL94_001345"
     CDS             complement(721..1674)
                     /gene="tal"
                     /locus_tag="KJL94_001345"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD4409766.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1953..3382
                     /locus_tag="KJL94_001346"
                     /pseudo
     CDS             1953..3382
                     /locus_tag="KJL94_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3452..4228
                     /gene="yaaA"
                     /locus_tag="KJL94_001347"
     CDS             3452..4228
                     /gene="yaaA"
                     /locus_tag="KJL94_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBD4409767.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4381..4677)
                     /locus_tag="KJL94_001348"
     CDS             complement(4381..4677)
                     /locus_tag="KJL94_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD4409768.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KJL94_001349"
     CDS             complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KJL94_001349"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBD4409769.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KJL94_001350"
     CDS             complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KJL94_001350"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBD4409770.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KJL94_001351"
     CDS             complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KJL94_001351"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBD4409771.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9600..9719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KJL94_001352"
     CDS             complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KJL94_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBD4409772.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9934..10620)
                     /locus_tag="KJL94_001353"
     CDS             complement(9934..10620)
                     /locus_tag="KJL94_001353"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD4409773.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11020..11160)
                     /locus_tag="KJL94_001354"
     CDS             complement(11020..11160)
                     /locus_tag="KJL94_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409774.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11256..11972
                     /gene="arcA"
                     /locus_tag="KJL94_001355"
     CDS             11256..11972
                     /gene="arcA"
                     /locus_tag="KJL94_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBD4409775.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KJL94_001356"
     CDS             complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KJL94_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBD4409776.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KJL94_001357"
     CDS             complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KJL94_001357"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBD4409777.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KJL94_001358"
     CDS             complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KJL94_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBD4409778.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KJL94_001359"
     CDS             complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KJL94_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBD4409779.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16251..17120
                     /gene="robA"
                     /locus_tag="KJL94_001360"
     CDS             16251..17120
                     /gene="robA"
                     /locus_tag="KJL94_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBD4409780.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KJL94_001361"
     CDS             complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KJL94_001361"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBD4409781.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17816..18336
                     /gene="yjjX"
                     /locus_tag="KJL94_001362"
                     /pseudo
     CDS             17816..18336
                     /gene="yjjX"
                     /locus_tag="KJL94_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KJL94_001363"
     CDS             complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KJL94_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBD4409782.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KJL94_001364"
     CDS             complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KJL94_001364"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBD4409783.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20985..22652
                     /gene="ettA"
                     /locus_tag="KJL94_001365"
     CDS             20985..22652
                     /gene="ettA"
                     /locus_tag="KJL94_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBD4409784.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22759..22926)
                     /locus_tag="KJL94_001366"
     CDS             complement(22759..22926)
                     /locus_tag="KJL94_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409785.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(23009..23695)
                     /gene="nadR"
                     /locus_tag="KJL94_001367"
                     /pseudo
     CDS             complement(23009..23695)
                     /gene="nadR"
                     /locus_tag="KJL94_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
     gene            complement(23716..25098)
                     /gene="radA"
                     /locus_tag="KJL94_001368"
     CDS             complement(23716..25098)
                     /gene="radA"
                     /locus_tag="KJL94_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBD4409786.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25147..26115)
                     /gene="serB"
                     /locus_tag="KJL94_001369"
     CDS             complement(25147..26115)
                     /gene="serB"
                     /locus_tag="KJL94_001369"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBD4409787.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26221..26865
                     /locus_tag="KJL94_001370"
     CDS             26221..26865
                     /locus_tag="KJL94_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBD4409788.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            26893..27908
                     /gene="lplA"
                     /locus_tag="KJL94_001371"
                     /pseudo
     CDS             26893..27908
                     /gene="lplA"
                     /locus_tag="KJL94_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            27940..28203
                     /locus_tag="KJL94_001372"
     CDS             27940..28203
                     /locus_tag="KJL94_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4409789.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28364..29083)
                     /gene="deoD"
                     /locus_tag="KJL94_001373"
     CDS             complement(28364..29083)
                     /gene="deoD"
                     /locus_tag="KJL94_001373"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBD4409790.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29140..30363)
                     /gene="deoB"
                     /locus_tag="KJL94_001374"
     CDS             complement(29140..30363)
                     /gene="deoB"
                     /locus_tag="KJL94_001374"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD4409791.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30415..31737)
                     /gene="deoA"
                     /locus_tag="KJL94_001375"
     CDS             complement(30415..31737)
                     /gene="deoA"
                     /locus_tag="KJL94_001375"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBD4409792.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(31864..32643)
                     /gene="deoC"
                     /locus_tag="KJL94_001376"
     CDS             complement(31864..32643)
                     /gene="deoC"
                     /locus_tag="KJL94_001376"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBD4409793.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            32901..34442
                     /locus_tag="KJL94_001377"
     CDS             32901..34442
                     /locus_tag="KJL94_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBD4409794.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34414..35277
                     /locus_tag="KJL94_001378"
     CDS             34414..35277
                     /locus_tag="KJL94_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBD4409795.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35491..36273)
                     /locus_tag="KJL94_001379"
     CDS             complement(35491..36273)
                     /locus_tag="KJL94_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4409796.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36270..37343)
                     /locus_tag="KJL94_001380"
     CDS             complement(36270..37343)
                     /locus_tag="KJL94_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBD4409797.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37465..37626)
                     /locus_tag="KJL94_001381"
     CDS             complement(37465..37626)
                     /locus_tag="KJL94_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBD4409798.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(37743..38348)
                     /gene="osmY"
                     /locus_tag="KJL94_001382"
     CDS             complement(37743..38348)
                     /gene="osmY"
                     /locus_tag="KJL94_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBD4409799.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(38741..40330)
                     /gene="prfC"
                     /locus_tag="KJL94_001383"
     CDS             complement(38741..40330)
                     /gene="prfC"
                     /locus_tag="KJL94_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBD4409800.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40421..41098)
                     /gene="yjjG"
                     /locus_tag="KJL94_001384"
     CDS             complement(40421..41098)
                     /gene="yjjG"
                     /locus_tag="KJL94_001384"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBD4409801.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41113..41559)
                     /gene="rimI"
                     /locus_tag="KJL94_001385"
     CDS             complement(41113..41559)
                     /gene="rimI"
                     /locus_tag="KJL94_001385"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBD4409802.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(41528..41941)
                     /gene="holD"
                     /locus_tag="KJL94_001386"
     CDS             complement(41528..41941)
                     /gene="holD"
                     /locus_tag="KJL94_001386"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBD4409803.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42044..43075
                     /gene="rsmC"
                     /locus_tag="KJL94_001387"
     CDS             42044..43075
                     /gene="rsmC"
                     /locus_tag="KJL94_001387"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBD4409804.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43352..43438
                     /locus_tag="KJL94_001388"
     tRNA            43352..43438
                     /locus_tag="KJL94_001388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43386..43388,aa:Leu,seq:cag)
     gene            43467..43553
                     /locus_tag="KJL94_001389"
     tRNA            43467..43553
                     /locus_tag="KJL94_001389"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43501..43503,aa:Leu,seq:cag)
     gene            43588..43674
                     /locus_tag="KJL94_001390"
     tRNA            43588..43674
                     /locus_tag="KJL94_001390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43622..43624,aa:Leu,seq:cag)
     gene            complement(43713..43949)
                     /locus_tag="KJL94_001391"
     CDS             complement(43713..43949)
                     /locus_tag="KJL94_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBD4409805.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44090..44878
                     /gene="fhuF"
                     /locus_tag="KJL94_001392"
     CDS             44090..44878
                     /gene="fhuF"
                     /locus_tag="KJL94_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBD4409806.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(44916..45593)
                     /gene="bglJ"
                     /locus_tag="KJL94_001393"
     CDS             complement(44916..45593)
                     /gene="bglJ"
                     /locus_tag="KJL94_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBD4409807.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(45551..>46118)
                     /locus_tag="KJL94_001394"
     CDS             complement(45551..>46118)
                     /locus_tag="KJL94_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD4409808.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        10923 a        12560 c        11617 g        11018 t
ORIGIN      
        1 ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc
       61 agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg
      121 tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca caccttccag
      181 cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct gtttgcgaat
      241 cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat
      301 ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac
      361 gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc
      421 caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc
      481 cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg gaatgccttt
      541 atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt
      601 attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca ctgcgaaggg
      661 agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg ctaagaatga
      721 ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa acttacggat
      781 accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga
      841 ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt tacgttcgat
      901 agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcaca
      961 gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc
     1021 ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc
     1081 tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac
     1141 aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag cgaaggagaa
     1201 cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag cacggatacc
     1261 ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg catcgttgta
     1321 gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc
     1381 atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac
     1441 tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc
     1501 ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat
     1561 gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc
     1621 agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt
     1681 atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac
     1741 gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc
     1801 tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg ctacgcagaa
     1861 gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa
     1921 agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc tttattaaca
     1981 gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt
     2041 ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt cttaaaaata
     2101 gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcgg
     2161 cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc ggtggaccag
     2221 gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg tttgccgaat
     2281 gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt ggcggaccgg
     2341 catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt
     2401 tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt gcccgagccc
     2461 tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct gtctttgctc
     2521 tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt
     2581 tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat atcgggcaac
     2641 ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg
     2701 gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt
     2761 ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca tcctggcctc
     2821 cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac accctggtca
     2881 tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc
     2941 tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tagggtgctg
     3001 aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt
     3061 atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc
     3121 ggatctgcac cttcgcaacg atcatcggcg gcaccttgct aagccttccg ctgatgtggc
     3181 aactggcaga tatataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct
     3241 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     3301 cccggtgttt gatccgttgc gttatccgga tatcggtcgc caactttctc cggacgcgtg
     3361 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     3421 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     3481 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     3541 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
     3601 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     3661 tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
     3721 cttgcaggcc gagaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     3781 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     3841 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     3901 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
     3961 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
     4021 gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa
     4081 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
     4141 gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact
     4201 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
     4261 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga
     4321 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
     4381 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
     4441 gtggcgcggc ggtgacggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
     4501 ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc
     4561 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc
     4621 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
     4681 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga
     4741 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct
     4801 ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa
     4861 acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa
     4921 cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag
     4981 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
     5041 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
     5101 acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat
     5161 tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat
     5221 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
     5281 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
     5341 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt
     5401 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
     5461 gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
     5521 caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc
     5581 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
     5641 agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc
     5701 tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc
     5761 agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg
     5821 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
     5881 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
     5941 cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga
     6001 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
     6061 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
     6121 tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta
     6181 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
     6241 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
     6301 acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcactaccg cgccgatttc
     6361 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc
     6421 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
     6481 gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
     6541 aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca
     6601 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
     6661 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc
     6721 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt
     6781 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
     6841 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
     6901 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
     6961 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
     7021 tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc
     7081 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
     7141 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
     7201 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
     7261 tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat
     7321 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
     7381 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
     7441 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
     7501 gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat
     7561 ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac
     7621 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
     7681 acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc
     7741 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
     7801 actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgta acaacgtgga
     7861 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
     7921 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
     7981 acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac
     8041 tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct
     8101 tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
     8161 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
     8221 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
     8281 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
     8341 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
     8401 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
     8461 gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg
     8521 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
     8581 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
     8641 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
     8701 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
     8761 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
     8821 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga
     8881 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
     8941 ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg
     9001 cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc
     9061 gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt
     9121 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
     9181 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
     9241 ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag
     9301 ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg
     9361 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
     9421 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
     9481 gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg
     9541 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
     9601 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
     9661 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
     9721 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
     9781 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
     9841 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
     9901 tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
     9961 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg
    10021 taaccagtcg accagtttta tgtcatctgc cactgccaga gccgtcagca atgccatgac
    10081 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
    10141 ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga
    10201 aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt
    10261 agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt
    10321 ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact
    10381 acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa
    10441 aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc
    10501 caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
    10561 cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat
    10621 attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc
    10681 ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa
    10741 aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc
    10801 ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt
    10861 tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
    10921 agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
    10981 aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt
    11041 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
    11101 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
    11161 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
    11221 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
    11281 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
    11341 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
    11401 acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac
    11461 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
    11521 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
    11581 aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
    11641 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
    11701 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
    11761 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa
    11821 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
    11881 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
    11941 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
    12001 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
    12061 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt ctatattacg
    12121 agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
    12181 gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgaggat
    12241 aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
    12301 cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc
    12361 cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg
    12421 taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag
    12481 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
    12541 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
    12601 accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc
    12661 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
    12721 ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt
    12781 cagtgccata ttcagctgca ggttctgctt acgccagtcc ccttcgggta aagggatatg
    12841 cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt
    12901 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    12961 gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc
    13021 ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc
    13081 ttccacgttc tgattgccat caaccatcaa tgactcaggt aacaaaaaat ggataaaact
    13141 tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat
    13201 agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc
    13261 cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat
    13321 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    13381 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    13441 ctatgtgaag tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg
    13501 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc
    13561 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    13621 cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc
    13681 cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag
    13741 taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg
    13801 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    13861 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    13921 tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt
    13981 gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    14041 acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat
    14101 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag
    14161 ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata
    14221 gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    14281 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac
    14341 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc
    14401 cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt
    14461 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    14521 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata
    14581 gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg
    14641 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    14701 gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct
    14761 tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc
    14821 caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg
    14881 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    14941 ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct
    15001 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac
    15061 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    15121 tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag
    15181 aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac
    15241 ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    15301 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    15361 aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc
    15421 tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc
    15481 tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc
    15541 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    15601 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    15661 ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    15721 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc
    15781 ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    15841 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    15901 tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    15961 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    16021 atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg
    16081 tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc
    16141 ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    16201 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    16261 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc
    16321 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    16381 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    16441 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    16501 ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc
    16561 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca
    16621 caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga
    16681 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct
    16741 ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa
    16801 cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc
    16861 aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat
    16921 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    16981 acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat
    17041 actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    17101 tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    17161 tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca
    17221 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tacccatgct ggtaatccga
    17281 gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc
    17341 cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca
    17401 ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt
    17461 tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt
    17521 cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg
    17581 tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac
    17641 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    17701 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    17761 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    17821 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    17881 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    17941 ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc
    18001 ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga
    18061 cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc
    18121 aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc
    18181 cgtgagtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    18241 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    18301 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    18361 tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    18421 tcaatcgctt ttcagcaaca cctcttccag ccactgacgc agctccacgg gcgcggcttt
    18481 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    18541 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    18601 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    18661 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    18721 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    18781 tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    18841 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg
    18901 tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    18961 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    19021 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    19081 taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc
    19141 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    19201 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    19261 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    19321 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    19381 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    19441 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggta
    19501 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    19561 gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc accgatgcgt
    19621 tgtgctgcaa ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa aatctctttc
    19681 gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct
    19741 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    19801 gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    19861 gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa acgccaggcg
    19921 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac
    19981 gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa cgccaccgcc
    20041 gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta
    20101 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    20161 cctgccagca ctgttaccag gcctgtgtta cccgctttca tcgccagacg gatacgctct
    20221 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    20281 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    20341 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    20401 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    20461 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    20521 accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc caggtaggga
    20581 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ctgtcgatta
    20641 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    20701 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    20761 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    20821 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    20881 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct acacttcgcc
    20941 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    21001 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    21061 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    21121 ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    21181 agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg
    21241 tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa cgcctggatg
    21301 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    21361 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    21421 gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac ctctccggtg
    21481 gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    21541 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa cgcttcctgc
    21601 acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc gataacgttg
    21661 caggctggat cctcgaactg gaccgcggtg aaggtattcc gtgggaaggt aactactcct
    21721 cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    21781 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    21841 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac cagaaacgta
    21901 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    21961 tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg agcttctcga
    22021 tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    22081 tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg ggtgaaacgg
    22141 tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    22201 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    22261 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt ggcgaacttt
    22321 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    22381 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    22441 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    22501 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    22561 actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg ctggagccga
    22621 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgcgaac
    22681 gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta ggcctgataa
    22741 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc gtgaacacct
    22801 tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc gtgcaggttt
    22861 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    22921 actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct cccccatcat
    22981 ctcccgcacc agctccacgc agcgcaggaa acggcttcac ttcggtagga atatattccc
    23041 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    23101 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    23161 ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt
    23221 tgctccacac atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    23281 tacggatatt tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg
    23341 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    23401 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    23461 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa
    23521 attcaagccc caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt
    23581 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    23641 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    23701 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    23761 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    23821 ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg
    23881 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    23941 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg ccgctggttt cggttacctt
    24001 cacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    24061 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    24121 cggattcgcc attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    24181 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    24241 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    24301 ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc
    24361 atcgccatcc agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    24421 cgaaccatct ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    24481 acgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    24541 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    24601 ggcaatcagg cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    24661 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    24721 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc
    24781 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc ctcctagtac
    24841 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    24901 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    24961 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    25021 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    25081 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc
    25141 aggcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    25201 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    25261 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    25321 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    25381 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    25441 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    25501 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa
    25561 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    25621 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    25681 ttcggttacc tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca
    25741 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    25801 cgggattttc cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    25861 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    25921 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    25981 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    26041 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    26101 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    26161 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    26221 atggctcgca caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt
    26281 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    26341 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    26401 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    26461 ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    26521 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    26581 ggcggctatt ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc
    26641 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    26701 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    26761 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    26821 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    26881 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    26941 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    27001 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    27061 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc
    27121 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    27181 gataaaactc tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc
    27241 gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca
    27301 ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt gctactcaat
    27361 gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    27421 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc
    27481 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcacgtgg
    27541 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    27601 cccgtcagag tagctgagag tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    27661 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    27721 cccgcgccca ggtatttacc gacagcctca acctcgcgcc gctggaagcc ctcgccgggc
    27781 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    27841 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    27901 taaggtaact cgctgattaa aatatccaag gaaggaaata tgatcgatct ggaaaaccag
    27961 gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc cgccgtgcgt
    28021 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    28081 ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatggcagaa
    28141 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    28201 tgaatagatt cgtgaataga tacttgaatg ttttttattt tctatccata tgaaaaataa
    28261 tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc
    28321 ccactccggc ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca
    28381 gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    28441 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    28501 cgccaaactc tgcagccacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    28561 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    28621 acaggttacc cacgcgagca tcaacgccca gtgctttagc tgcatctact gcgttacgca
    28681 ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    28741 tggaatcggt gcaggccccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    28801 ctgcgccaca ggaaccaacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    28861 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    28921 ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    28981 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    29041 cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta tcctttttgt
    29101 gacataacaa aggcggagtc aacgctccgc ccaaatccat cagaacatgg ctttgccata
    29161 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    29221 acggtgaccc agtgagcccg gttttacttt cgggccatat accagtaccg gaatgtgttc
    29281 acgcgtgtgg tcagtaccgg tccaggtcgg atcgcaaccg tggtcagcgg tgaggatcag
    29341 gatgtcgtca tcgcgcagca gagacatcag ctccggcaga cggcggtcga acagttccag
    29401 acccgcagca taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa
    29461 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    29521 cagcgcgtcc aagccagtcg ctttcacttt tttggtgata ccgcagttgg cgtagatgtc
    29581 tgcaatttta ccgacagaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    29641 ggtcggtgct ggcggctcaa cagccaagtc gtgacggtta ccggtacgct ggaagttacc
    29701 agctttgtca ccgataaacg gacgagcgat aacacgaccg atattgtagc cgccgttggt
    29761 cagctcttca cgggcgattt cgcacagttc gtagagttta tccagaccga aagtttcttc
    29821 atggcaggca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    29881 catgtgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    29941 gtaacccggc agattagcgc gttcgaccag tttatccagc agctcttgcg ggaagctgtt
    30001 ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg acaccggcga tttcccagtg
    30061 accagacggg gtatctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    30121 ttcagcgttg ccgtccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    30181 cagccccaga cgggtcagat ttggcagatt gagcggccct ttacgaccat tatcagcttc
    30241 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    30301 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    30361 catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat accgttattc
    30421 gctgatacgg cgatagacag ttggtgtgct ttccggtgcg ttatcggcaa gtttaattgc
    30481 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcga ggataaccgc
    30541 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    30601 gtaatcgatg gtgtcagatg cctgacgacg accgccgccc attgcaacca ctgccatccc
    30661 cagcgcgcgg gtatccattt cactgacaaa accttcggta tcagcataga ctgctttcgt
    30721 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    30781 tgccgctacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    30841 tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg gagatcagca tctccacgca
    30901 cagcgccatc gtgacatcaa acagacgcgg gttacgatac tcacccgtca ggaactgcac
    30961 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatgtcggt
    31021 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    31081 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac
    31141 cagcgcgtcc agaccttccg ccagtttctt cgccaggatc gaggcagtga tcagcgggat
    31201 ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatcag ccggagccag
    31261 tgaactggtc tgaccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    31321 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    31381 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccgcaggctg cgaccatcgg
    31441 ccccaacatc agcgaagtga catcgccgac gccgccggtc gagtgtttat caacaatcgg
    31501 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt
    31561 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    31621 aatctgccct tcggaaatag tgttgtcgcg aataccgttg ataaagaaac gaatttcttc
    31681 atcgctcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    31741 aacctcccga ggaaaagagt atgaggcggc ttttgccgga tgcgtctacg ccttatccgg
    31801 cctacgggta gatacgtctc gtaggcctga taagcgcagc gcatcaggcg taaagcatct
    31861 tacttagtag ctgctggcgc tcttaccgtc gccgtggccc agcgctttca gcaggcttgc
    31921 cagcaggctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag caccgaacag
    31981 ttcatctgcg atggcgagat atttctgcgc atcttccgca gtacgcacgc cgcccgccgg
    32041 tttgaaacca acggtttttt ctacgcccat atcacggatc acttccatca tgatgcgagc
    32101 gctttccggc gtcgcgttca cagccacttt accggtagag gttttgatga agtccgcacc
    32161 cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca gttcgccggt
    32221 ttcgatgatc actttcagca gtacattcgc tgccgcgcaa gcctctttac aggctttcac
    32281 caggtcaaaa ccaacctgct cgtcacccgc catcagcgcg cggtacggga acaccacgtc
    32341 aacttcatca gcaccgtagg cgattgccgc acgggtttct gccagcgcga tttcgatgtc
    32401 gtcgttaccg tgtgggaagt tggttaccgt agcgatacgg atttccgggg tgccctgctc
    32461 tttcagagtt ttgcgagcaa tcgggataaa acgaggatag atacagatag cggcggtatt
    32521 gccgaccgga gttttggcct gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc
    32581 attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt
    32641 catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt tagtattcta
    32701 acatctactc cgcaacacac ttcgatacac atcacaatta aggaaatcta cttacaagtt
    32761 tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg cgtcagtggt
    32821 ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc gcatcacctt
    32881 cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc
    32941 agcaaattgt caccagccca gtgcttagcc cggagcagaa gcgccatttt ctggcactgg
    33001 aagcagaaaa caatctgcct tacccacagc ttcctgccga agcccgccgc gcgctggatg
    33061 aaggtgtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac
    33121 ccgattacgc tcgttttctg gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag
    33181 atcttgatga cgcactctct ctgctgacca ttctttacca ccacgtaccg tcggtcacat
    33241 cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt agaattctaa
    33301 cacaaaacga gatcgatgtt cgaataaaac gtttctggcg ttacctcgac agaaccctgc
    33361 cagatgcctt tatgcacgcc aatatcggcc cgtctgattc gcccattacc cgcgcgatct
    33421 tacgtgcaga cgcgcaggtt tcaccgaacc tgacctttat ctacgatcct gaaatcaccc
    33481 ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg
    33541 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    33601 acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca
    33661 ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc
    33721 agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    33781 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    33841 ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgagaaagaa gggattgccg
    33901 cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    33961 cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg
    34021 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg
    34081 atgagccaga tccgatcacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt
    34141 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaacccg caggcactgg
    34201 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    34261 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc
    34321 gcgccgaagg ttcacgcacc aacaccacct ggctgggcga agaagccgca cgcaacactc
    34381 gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    34441 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    34501 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ttgcaatgac
    34561 tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg
    34621 gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat
    34681 gccacaccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt gcgcaaagcg
    34741 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca actgccgttt
    34801 gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccatct cacctgtctg
    34861 gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgtgac
    34921 ggcgcaatgc tcgatctcaa agcatggggt agcgaatgtc atcaacaatt aaccggacgc
    34981 gataatcaac agattaagcg cagcatctat ttgctggcag agcgcggcaa gctggcggaa
    35041 ctgcgtttgc tgatgatccc aggccaggtg gattatttgc aacacatcga agaactggcg
    35101 gcgtttatca agggacttgg tgatgttccg gttcgcctga acgcgtttca tgcccacggc
    35161 gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct
    35221 gatgcattaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat
    35281 gccggatacg gcctacggtt cggcacagac ttgtaggcct gataagacgc gtcaagcgtc
    35341 gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt
    35401 tcggcacaaa cctgtaggca tgataagacg cgttaagcgt cgcatcaggc attatgcgcc
    35461 gactgccgga tgctgcctac gaagggcaaa ctacccacga acgttaaaca ccgcatacgt
    35521 gttattaagc aacacttcgg caatctcatc cgcgggttcc gggcgtaact cgcaaagcac
    35581 ggcaaacaca cgggcagcct gttccgggcg gttaggctgc ccctgaaaac cgttgagcgg
    35641 catatccggc gcatcggttt ccagcaacaa cgatgccagc ggtaattttg cgatgacatc
    35701 gcgggtttta ctggcgcgtg gataggtgat agtaccgcct acgccgattt tgtagcccag
    35761 ctgtacaaac cgttcagcct gttgctggct gccagaaaat ccgtggacaa caccagcacg
    35821 cggcaaatcg tggtgtttaa gatgcatcgc cagtttgtcg tgcgtgcgcc gtgaatgcag
    35881 gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta accactgctg
    35941 cctctcaaat tgcggatcgt cgccgaagag atccagaccg atctccccca ccgccaccac
    36001 cttcgccgga cgcctttcca gcgcctgctg tagctgctca agagacacat cgctatgttt
    36061 ttccaacata ccaggatgca agcccaatgc ggcatacagc ggttgataat tttccgctaa
    36121 cgcctgcacg cgggcaaaat tttccgcctc tgtcgccgga acaatgatct tgcctacgcc
    36181 cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa
    36241 atggcagtga gtgtcgataa aacggcaaat caagccagat cctcattgtt aaatgtcggg
    36301 tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg tatcgttggc gactggcgca
    36361 ggcggaatga caatcgattc cggcgtcacc actggcacca gatggcgggt aagcggcgct
    36421 ttttcagtta atagcttgcc aaccgtggcg aggaaataac gaccgcataa acgcccaagt
    36481 ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg taatggcttc
    36541 ggcggataaa tttcggatat ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg
    36601 tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag gctactgtca
    36661 cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg tgacggcaca
    36721 gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac cggaatcgca
    36781 tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct acgataaaag
    36841 ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt tggtaaaaag
    36901 taattcggct cgtagtcatc ctgacgacag gcgcacatat aaaacgattt gccgctgtca
    36961 aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc ctccaccagc
    37021 cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa aaattcgcgt
    37081 tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca gataaacgcc
    37141 gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt aaactgcgcg
    37201 cgcataaact catccagcac gccagccgtg aaaattccac gctgtccgcc gccttcgcac
    37261 accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat attaccaagc
    37321 gttacaggta ttcgctgccc cacttctgcc ttcctgtttt tgttgtgaaa gaaagacaaa
    37381 gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact ggctttggat
    37441 aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca aactcactag
    37501 gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg taccggccag
    37561 accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga tgccccaacg
    37621 aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca
    37681 ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg
    37741 aattacttag ttttcagatc atttttaacg cttttcacac catctaccgc tttggcgata
    37801 ctttcagcac ggtcactttg tgcctgagaa tctacggtac cggagagctg aaccacgcca
    37861 tcggtggttt caactttcac tttacgtgaa gggacgatat cgtccgccag cagtttggct
    37921 ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct tcaccgagcc ttctttagcg
    37981 tcgcgaacgt gcagtttgtc gctgacagag gtcacgcctt caacgccttt cgccactttc
    38041 actgcctctt cggcctgggc ctgactttca acgaaaccgc tcagggtcac gactttttga
    38101 tcggttttga cagagatatc ggtgctcttg atgttgtcat gatccaccag cgccgccttc
    38161 actttcgcgg tgatggcgct gtcatccatg aaattaccga ctttattcat agagctatcg
    38221 actttttgcc ctgcgctttc attggtagtc tgcgcgttgt tttccgcgta ggcagagccg
    38281 gtcgcgacgg cagaggtcaa cattacagcc agcagagttt tcgaaatctt cagtcttgtc
    38341 atagtcatcg atttattcct gtatgtttgc tcgtaatttg agcctagcaa cacgaggttg
    38401 cattgctgaa tagggagaga cttcaccctc tacagaagtc aatggtcgcc atcacaaaag
    38461 cgatgggtga tgaataacga ccattacagc ctctgaatca gttattaata tcggtagaat
    38521 gacaatcgac ggctttagat actgatatct acgcattgaa cggtatttaa cgccatcaga
    38581 aatgtcatca ctttgttaaa tatagatcac aattttgaaa ccgctcggga tatcagcgaa
    38641 aacataagca aaagtgaatg ttttaagaac attccgtaag cggctaataa ggaagggaaa
    38701 ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa ttaatgctcg cgggtctggt
    38761 ggaactgaac gtccggataa cgttcctgtg ccaggcgcag gttgaccatg ctggtagcga
    38821 tgtaagcgag gttatcgccg ccatcaagcg ccagttggct ttcgttctta cgcttgaact
    38881 cttcgaattt cttcgcgtct gcacattcta cccagcgggc agtggcaacg ttgactgact
    38941 catacactgc ttcaacgttg tattcgctct tcaggcgcgc taccaccaca tcaaactgca
    39001 gcacaccaac cgcaccaacg atcagatcgt tgttggagat tggacggaac acctgcaccg
    39061 cgccctcttc ggaaagctgt accagccctt tgagcagctg tttttgcttc agcggatctt
    39121 tcaggcggat acgacggaac agttctggtg cgaagttcgg aataccggtg aacttcatca
    39181 tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc gtggttgtgc aggccgagga
    39241 tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc cgccataaag gtcagcgcgt
    39301 cggagatcac cacatctttc gcagtgcgca cctggcgcag tttcatgcct ttttcatatt
    39361 taccggacac cacacgcata aacgccacgc ggtcgcggtg tttcggatcc atgttggcct
    39421 gaattttaaa tacgaagccg gtaaatttat cttcgctcgc ttctacggta cgggtatcgg
    39481 tctgacgcgg catcggcgca ggcgcccact ccaccaggcc attcagcata tgatcaacgc
    39541 cgaagttacc cagcgcagta ccgaagaata ccggagtgat ttcgcccgca aggaacagct
    39601 ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag ttcgtcacgc agctgctgtg
    39661 ccagatcttc accaaccgca gcatcgagat ccgggttatt cagcccttta acaatgcgga
    39721 cttcctgaat ggtgtgccct ttaccgctct gatagagata ggtttcatct ttataaaggt
    39781 ggtaaacgcc tttaaacagc ttgccgcagc caattggcca agtgatcggt gcgcagccaa
    39841 ttttcagctc gttctcaact tcatcgagca gttccatcgg gtcgcggata tcacggtcaa
    39901 gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg ggtaacttcc atcagcttac
    39961 gggtacgatc ttcaacacct tttgcggcgt cgataaccat caggcagcag tccaccgccg
    40021 tcagggtacg ataggtatct tccgagaagt cttcgtgccc cggggtgtcg agcaggttaa
    40081 ccaggcaatc gtgatacgga aactgcatca cagacgtagt aatggagatc ccacgctgct
    40141 tttccatctc catccagtcc gacttagcgt gctggttgga accacggcct tttactgtac
    40201 cggcggtctg aatggcctgt ccgaacagca gcaccttctc ggtgatggta gttttaccgg
    40261 cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc cacctctttc aaataaggag
    40321 acaacgtcat aattctttct tcttgagtaa gcggcatcgc gccgcgcttg ttgaaaacga
    40381 aaaattgcgg ctattttacc catcaacggg ggggaggcaa tcagtgttta cacaggagct
    40441 gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc gatgccttct ggttgctcgc
    40501 gattgtgcgc attcagccag caggtcgcaa gcccggcgtt gatgccaccg agaatatcgg
    40561 actcggcagt gtcgccaacc atcagcacgc gtgaacggtc aggattgccc gcctgttcca
    40621 gcgcataatc gaaaattttc ttattcggtt tggcaacgcc aacttcttcg gaaatcacca
    40681 gcaaatcgaa gtaatcacgc aggcccgtcc gttccagacg cacttgctgc aaggcgctaa
    40741 aaccgttggt gatgatgccg attttagcgt tgctacgaat ggcgttaagc agagaaaccg
    40801 cgcccggcaa cggcgtacag atttccgcca tcgcattaat aaaggcatcg ttaagcaagc
    40861 ctgcttcaac tttcagccgt tcggcccagc tctcgaaacg cccgtgctga agctgtaatg
    40921 aagtgatcgc gccgttttga taatccaccc acagtggctt gttaacggcc tgatagtcct
    40981 gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa catccgctgc aggccggtga
    41041 atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa aatccagtcc cacttcatta
    41101 ttccaccttg tcttacatac tgattggcaa cgccatgatg atggcgtctt cgcgaccgtc
    41161 cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta aagcctaaac tttcgtacag
    41221 ggcaatggcg gcagcatttg aggcacggac ttccagccat agtgtcgcca cgccgcgttt
    41281 ttccagttcg tcgatcagat gttccagcag cgccctaccc agcccctgac gctgatagtc
    41341 gggatcgacc gcaatattga acaatgtagc ttcatccagc accacttgcg taatcgcaaa
    41401 cgccgccatt ttgccgtttt gcgttaactg aaagttgaga taacgctcgc cctggttgct
    41461 ggcaaaggtt ttttcactcc acggaaagga gtgggcgcgt tgttcaatgt ggtaagccgc
    41521 tggtaaatca gtcgtttcga gggaagaaat cgtgttcata tgtgcaaatt tgttgccata
    41581 acgcggcgcg tgccgttggg tttgcccgta attcggtgag cgccggtgat gccacctgag
    41641 cgccttccaa tgatagcggt tcgtcagtac ccaaccgcca gctgttgcag cgactgcctt
    41701 gcggcagcat cgcgattttt tctggcgtca gttgcagcac ttgatcgggg ctgacggtta
    41761 atgcgcgcag aacatcgctc actaaaggat cagtcagggc gggaagatcg tttgccacca
    41821 tcaccagacg gacgtgtgcc gggctcgcaa tggcaatctc cccctgcaac gcgccagggc
    41881 gacgcagcga ccactgggta atgcccagtt gctgtaactg ccagtctcgt cgggatgtca
    41941 tagcgaatcg ctcctgttgt caggggcgca aatatagcaa attcgtcgat accgcgccaa
    42001 catatggcta taatcgccgc cagtatcaat tgaggagcat tccatgtctg catttacccc
    42061 ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa caaagccgta ttctgtttgc
    42121 cggagactta caggatgacc tgcccgcgcg tttagatacc gcggccagcc gtgctcatac
    42181 ccagcaattc caccattggc aggtgttaag ctgccagatg ggggataacg cccgttttag
    42241 tctggtcgcc acggcgaatg atgtcgcaga ttgcgatacg ctgatttact actggccgaa
    42301 gaacaaaccg gaagcccagt tccagttgat gaatttactt tctctgctgc cagtggggac
    42361 agatattttt gtcgttggcg agaaccgcag cggcgtgcgc agcgctgagc agatgctggc
    42421 agattatgcg ccgttgaata aagtcgacag cgctcgtcgc tgtggcctct attttggtcg
    42481 tctggaaaaa cagccagtat ttgatgcgga taaattctgg ggcgaataca gcgtcgatgg
    42541 cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac ggtctggatg tcggtagcca
    42601 gttgctgctc tcgacgttaa ctccgcacac gaaaggtaaa gtgctggatg tcggctgtgg
    42661 cgcgggtgta ctttcagttg cctttgcgcg ccattcgccg aaaattcgtc tcaccttgtg
    42721 cgatgtctct gccccggcgg tagaagccag ccgcgcaaca cttgcagcca acggtgttga
    42781 aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa ggttgttttg atatgatcat
    42841 ctccaacccg ccgttccacg atgggatgca aaccagcctg gatgcggcgc aaacgctgat
    42901 tcgtggcgcg gtgcgtcatc ttaatagcgg cggcgagctg cgaattgtag cgaacgcctt
    42961 cctgccttac ccggacgtgc tggatgagac atttggcttc catgaagtta tcgcgcaaac
    43021 cgggcgcttc aaggtatatc gcgccattat gacccgccag gcgaagaaag gttgagtatc
    43081 tgatatctgt cgccgcatac cgtgtcggat gcggcgtgaa cgccttatcc gacctacaaa
    43141 acctgcacgt tagcccttcg taggccagat aagacgcgcc agcgtcgcat ctggcatctg
    43201 cccccgcatg cctaccaaac ctgcacgcta gtttcctgat ggacattttt ccagcaatta
    43261 cacatcaggc gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt
    43321 ggtagcaatt ctttttaaga attgatggta tgcgaaggtg gcggaattgg tagacgcgct
    43381 agcttcaggt gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa
    43441 aaccacgttg atattgctcg cactgggcga aggtggcgga attggtagac gcgctagctt
    43501 caggtgttag tgttcttacg gacgtggggg ttcaagtccc ccccctcgca ccaaacgagg
    43561 cgatatcaaa aaaagtaaga tgactgtgcg aaggtggcgg aattggtaga cgcgctagct
    43621 tcaggtgtta gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaattatc
    43681 tttacttcct ttcttgtttc ttccctgttt tgtcatccct gattcaaatt cattatcatc
    43741 agcatgatgc cagcaataag cgccaggcac gatggtagca aaatgtaatg ccgcaactgt
    43801 ttgtgataca gcatcaccgg cgtcaatact aatcccatca gaatgacaat ccgccactga
    43861 tccggcgata aatccgcata catcagccct gcaatcagca atcccggcag caacactccc
    43921 cagccactgc ccagcgtacg ttgcaacatg gtttcatctc tttcattgat aatgaaaacc
    43981 aatatcatat gataattttt atcatttgca agccagagaa atcccttgct atcgagtaaa
    44041 cctatcgcta ggattagcaa tcattatcat ttagattact atcacgatta tggcctatcg
    44101 ttccgcaccg ctctatgaag atgtcatctg gcgaacgcat ctccagccgc aggatgctgg
    44161 gcttgcacaa gccgtacggg cgatgatcgc caaacatcgt gaacatttgc tggagtttat
    44221 ccgcctggat gaacccgctc cgcttaacgc catgacgctg gcgcaatggt catcaccgaa
    44281 tgcgctaagt tctctactgg cggtctattc cgatcatatc tatcgcaacc aaccaacgat
    44341 gatccgcgag aacaaaccgc tgatctcact atgggcacaa tggtatatcg gcctgatggt
    44401 gccaccatta atgctggcgc tactgacgca ggaaaaagca ttagatgtgt cgccggaaca
    44461 tttccatgct gagtttcacg aaaccggacg cgtcgcctgt ttctgggtcg atgtgtgtga
    44521 agataaaaac gcaacaccac attcgccgca gcagcgaatg gaaacgttaa tcagccaggc
    44581 gctggttccg gttgtgcaag cactagaagc gaccggagag atcaacggca aacttatctg
    44641 gagtaatacc ggttatttga tcaactggta tctcactgag atgaagcaac tgctcggcga
    44701 ggctactgtt gaatcgctgc gccatgccct cttttttgag aaaacgctca cgaacggtga
    44761 agataatcca ctctggcgta ccgtggtact gcgcgacggc ctgctggtgc gccgcacttg
    44821 ctgccagcgt tatcgcttac cggatgtgca gcaatgtggc tattgtacgc tgaaatgaaa
    44881 gcactgccgg ggaagtaaac ccggcatcat gcggattaat agggatgcaa cacattactt
    44941 gcttcgcaat gccgcataca taacagaata tctgcggcct ccaacaaccc tgcatcagaa
    45001 ctgacgccca gtttcgacat cacattaaat ttatgcgctc ggatcgtttt gatattacgt
    45061 ttaagctgct cggcaatttg tgtcattgag tagccacgcg acataaagcg caatatttct
    45121 ctctccgtcg ggcttaacgt ccggctttgg ttaatgtacc actgattgtt cagtcggtcg
    45181 gtcgcctgac gtacaccatt taatgacaaa aagagttcct gatgaaaaat ctccagcgtt
    45241 gacgctttac ttaatacacc gtccagcggt gatggcgaca atgaaccaat cagccgagct
    45301 tcaatatcat catccacaat aactaaacgc cgggtacgcg gaaacttaat cgccagctca
    45361 gtcaggcaag ataatccctc tctgcgctca cttctcatgg cagaaaaaga aaaaatgacc
    45421 gccgcaaacg agatccgcga catggcatcc tgaaatgact cctgactggt ataggtatgg
    45481 agcttataag ggttgcccgc aaactttctg gataaactct caataccgat actactcatg
    45541 acgcattttt ctatgagtgc gacatttctc ttcttaattc ggctgtgttc cattctgcgg
    45601 catctccata aacagaaggc aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg
    45661 catacatttc agcgttgctg tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc
    45721 tgatagtttt attacttttc ttaagcaatg aagctatctg gttgatgccc catcctttac
    45781 ctaatagccg cagtaccttg cgctctgaaa gagtgagaat gacggagcga tagctaaaat
    45841 catgagtttc agtcattgcc ggagaggtca gcatggcgct gatacgctct gcgtgcgtat
    45901 tgccggaacg tacggtcttg accagattct caatgggttc aacatcagac aataacgtcg
    45961 ccgtagggca catgagcagt tctacagcct gggaatacca ggagcgtgac accatgaaaa
    46021 cccagtgaat ttcccgatat tgtgagatta aagaataata atgttcacaa acagaacgtg
    46081 gatcttcact ttcaccggct aaatcagcaa tgactaat
//
LOCUS       DAEBXD010000025        45794 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEBXD010000025 DAEBXD010000000
VERSION     DAEBXD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45794
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.025"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            104..2257
                     /locus_tag="KJL94_001395"
     CDS             104..2257
                     /locus_tag="KJL94_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD4409809.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2310..4040
                     /locus_tag="KJL94_001396"
     CDS             2310..4040
                     /locus_tag="KJL94_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409810.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4100..4591)
                     /gene="yajQ"
                     /locus_tag="KJL94_001397"
     CDS             complement(4100..4591)
                     /gene="yajQ"
                     /locus_tag="KJL94_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBD4409811.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4759..5670
                     /gene="panE"
                     /locus_tag="KJL94_001398"
     CDS             4759..5670
                     /gene="panE"
                     /locus_tag="KJL94_001398"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBD4409812.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5633..6223
                     /gene="yajL"
                     /locus_tag="KJL94_001399"
     CDS             5633..6223
                     /gene="yajL"
                     /locus_tag="KJL94_001399"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBD4409813.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6277..7725)
                     /gene="thiI"
                     /locus_tag="KJL94_001400"
     CDS             complement(6277..7725)
                     /gene="thiI"
                     /locus_tag="KJL94_001400"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBD4409814.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7931..8173
                     /gene="xseB"
                     /locus_tag="KJL94_001401"
     CDS             7931..8173
                     /gene="xseB"
                     /locus_tag="KJL94_001401"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBD4409815.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8173..9072
                     /gene="ispA"
                     /locus_tag="KJL94_001402"
     CDS             8173..9072
                     /gene="ispA"
                     /locus_tag="KJL94_001402"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBD4409816.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9097..10959
                     /gene="dxs"
                     /locus_tag="KJL94_001403"
     CDS             9097..10959
                     /gene="dxs"
                     /locus_tag="KJL94_001403"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBD4409817.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11014..11931
                     /gene="yajO"
                     /locus_tag="KJL94_001404"
     CDS             11014..11931
                     /gene="yajO"
                     /locus_tag="KJL94_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBD4409818.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12042..12560)
                     /gene="pgpA"
                     /locus_tag="KJL94_001405"
     CDS             complement(12042..12560)
                     /gene="pgpA"
                     /locus_tag="KJL94_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBD4409819.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12538..13515)
                     /gene="thiL"
                     /locus_tag="KJL94_001406"
     CDS             complement(12538..13515)
                     /gene="thiL"
                     /locus_tag="KJL94_001406"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBD4409820.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13593..14012)
                     /gene="nusB"
                     /locus_tag="KJL94_001407"
     CDS             complement(13593..14012)
                     /gene="nusB"
                     /locus_tag="KJL94_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBD4409821.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14032..14502)
                     /gene="ribE"
                     /locus_tag="KJL94_001408"
     CDS             complement(14032..14502)
                     /gene="ribE"
                     /locus_tag="KJL94_001408"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBD4409822.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14591..15694)
                     /gene="ribD"
                     /locus_tag="KJL94_001409"
     CDS             complement(14591..15694)
                     /gene="ribD"
                     /locus_tag="KJL94_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBD4409823.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15698..16147)
                     /gene="nrdR"
                     /locus_tag="KJL94_001410"
     CDS             complement(15698..16147)
                     /gene="nrdR"
                     /locus_tag="KJL94_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBD4409824.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16298..16837
                     /locus_tag="KJL94_001411"
     CDS             16298..16837
                     /locus_tag="KJL94_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD4409825.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17135..18019
                     /gene="tsx"
                     /locus_tag="KJL94_001412"
     CDS             17135..18019
                     /gene="tsx"
                     /locus_tag="KJL94_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBD4409826.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18196..18543)
                     /locus_tag="KJL94_001413"
     CDS             complement(18196..18543)
                     /locus_tag="KJL94_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBD4409827.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18672..19643)
                     /gene="secF"
                     /locus_tag="KJL94_001414"
     CDS             complement(18672..19643)
                     /gene="secF"
                     /locus_tag="KJL94_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBD4409828.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19654..21501)
                     /gene="secD"
                     /locus_tag="KJL94_001415"
     CDS             complement(19654..21501)
                     /gene="secD"
                     /locus_tag="KJL94_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBD4409829.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21529..21861)
                     /gene="yajC"
                     /locus_tag="KJL94_001416"
     CDS             complement(21529..21861)
                     /gene="yajC"
                     /locus_tag="KJL94_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBD4409830.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21884..23011)
                     /gene="tgt"
                     /locus_tag="KJL94_001417"
     CDS             complement(21884..23011)
                     /gene="tgt"
                     /locus_tag="KJL94_001417"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBD4409831.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23066..24136)
                     /gene="queA"
                     /locus_tag="KJL94_001418"
     CDS             complement(23066..24136)
                     /gene="queA"
                     /locus_tag="KJL94_001418"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBD4409832.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24229..24802
                     /gene="acpH"
                     /locus_tag="KJL94_001419"
                     /pseudo
     CDS             24229..24802
                     /gene="acpH"
                     /locus_tag="KJL94_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24807..26624)
                     /gene="malZ"
                     /locus_tag="KJL94_001420"
     CDS             complement(24807..26624)
                     /gene="malZ"
                     /locus_tag="KJL94_001420"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBD4409833.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26780..28153)
                     /gene="proY"
                     /locus_tag="KJL94_001421"
     CDS             complement(26780..28153)
                     /gene="proY"
                     /locus_tag="KJL94_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBD4409834.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28229..29548)
                     /gene="brnQ"
                     /locus_tag="KJL94_001422"
     CDS             complement(28229..29548)
                     /gene="brnQ"
                     /locus_tag="KJL94_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBD4409835.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29955..31250)
                     /gene="phoR"
                     /locus_tag="KJL94_001423"
     CDS             complement(29955..31250)
                     /gene="phoR"
                     /locus_tag="KJL94_001423"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBD4409836.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31308..31997)
                     /gene="phoB"
                     /locus_tag="KJL94_001424"
                     /pseudo
     CDS             complement(31308..31997)
                     /gene="phoB"
                     /locus_tag="KJL94_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32187..33389
                     /gene="sbcD"
                     /locus_tag="KJL94_001425"
     CDS             32187..33389
                     /gene="sbcD"
                     /locus_tag="KJL94_001425"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBD4409837.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33386..36529
                     /gene="sbcC"
                     /locus_tag="KJL94_001426"
     CDS             33386..36529
                     /gene="sbcC"
                     /locus_tag="KJL94_001426"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBD4409838.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36655..37836
                     /gene="araJ"
                     /locus_tag="KJL94_001427"
     CDS             36655..37836
                     /gene="araJ"
                     /locus_tag="KJL94_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBD4409839.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37979..38887)
                     /gene="mak"
                     /locus_tag="KJL94_001428"
     CDS             complement(37979..38887)
                     /gene="mak"
                     /locus_tag="KJL94_001428"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBD4409840.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39012..39923
                     /gene="rdgC"
                     /locus_tag="KJL94_001429"
     CDS             39012..39923
                     /gene="rdgC"
                     /locus_tag="KJL94_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBD4409841.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40001..40456)
                     /locus_tag="KJL94_001430"
     CDS             complement(40001..40456)
                     /locus_tag="KJL94_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409842.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40453..40788)
                     /locus_tag="KJL94_001431"
                     /pseudo
     CDS             complement(40453..40788)
                     /locus_tag="KJL94_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40943..41227)
                     /gene="ppnP"
                     /locus_tag="KJL94_001432"
     CDS             complement(40943..41227)
                     /gene="ppnP"
                     /locus_tag="KJL94_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBD4409843.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41299..41976)
                     /locus_tag="KJL94_001433"
     CDS             complement(41299..41976)
                     /locus_tag="KJL94_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBD4409844.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42237..42425)
                     /gene="yaiA"
                     /locus_tag="KJL94_001434"
     CDS             complement(42237..42425)
                     /gene="yaiA"
                     /locus_tag="KJL94_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBD4409845.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42475..42999)
                     /gene="aroL"
                     /locus_tag="KJL94_001435"
     CDS             complement(42475..42999)
                     /gene="aroL"
                     /locus_tag="KJL94_001435"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBD4409846.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43182..43640)
                     /locus_tag="KJL94_001436"
     CDS             complement(43182..43640)
                     /locus_tag="KJL94_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBD4409847.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43760..44569
                     /gene="proC"
                     /locus_tag="KJL94_001437"
     CDS             43760..44569
                     /gene="proC"
                     /locus_tag="KJL94_001437"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBD4409848.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44586..45701)
                     /gene="adrA"
                     /locus_tag="KJL94_001438"
                     /gene_synonym="dgcC"
     CDS             complement(44586..45701)
                     /gene="adrA"
                     /locus_tag="KJL94_001438"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBD4409849.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11907 a        11942 c        11200 g        10745 t
ORIGIN      
        1 gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa actatacttt
       61 ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca aatcattatt
      121 tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat cccctcccgc
      181 aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa cagcattatc
      241 tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta atccttgcga
      301 aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag cctatgccga
      361 caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta tgaccatcaa
      421 tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta caacagaatt
      481 atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa ctttaaaacc
      541 aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg acgaggagaa
      601 aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt cttcaactaa
      661 acagattgca tttgaaggat atacagacac agataacctc tccgattcta aatttatttc
      721 ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact ataatccaaa
      781 aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg tctatgaatc
      841 tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg aaaatggggc
      901 cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat gccctcattt
      961 ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac tcgatggtcc
     1021 tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc agcaaagtgt
     1081 gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta tacacaatag
     1141 aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg gtaagttact
     1201 gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt atgaaaatgg
     1261 gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc tggttaagca
     1321 atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca tactcgatgg
     1381 taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt ataagaatgg
     1441 aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct attcaatcga
     1501 atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca caggtaaact
     1561 aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag agtttaacga
     1621 tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac atggtacgga
     1681 ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg atcagaaatt
     1741 atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg attccagtgc
     1801 tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag ggattaaatg
     1861 gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag ctacagccta
     1921 caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc aacaagctgc
     1981 aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca tcggaaaaga
     2041 actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg cacaaggtaa
     2101 ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg ttgagagaaa
     2161 tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag aacctgcgct
     2221 taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac cacgtaaggt
     2281 tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg ttattaatga
     2341 ttttatctgt tttttattct catcaggttt tatccagcga acccactcag ccggatgttt
     2401 attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat accaccttca
     2461 gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc taccctgaac
     2521 gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa tattatctgg
     2581 atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta acttttttat
     2641 caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat atcaatagct
     2701 ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat gagaatgccc
     2761 tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat aaggcaataa
     2821 gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa gtcaccatgt
     2881 tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc attcattatg
     2941 ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt cgtgaatccc
     3001 attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc aacaaagaaa
     3061 aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa tcgaattact
     3121 taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag caagactatc
     3181 aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt tccccatcaa
     3241 caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg gaattgatga
     3301 gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat agtcagtatt
     3361 gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac aatgagctaa
     3421 tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta gaagactggg
     3481 atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc aaatatgctc
     3541 aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca cattctgaaa
     3601 gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt aatgatactg
     3661 ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat catatgattc
     3721 aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata aaagaggaaa
     3781 tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa aatgcagaat
     3841 gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca ataacaaaaa
     3901 tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat aagttggaaa
     3961 ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg gcatcaggaa
     4021 gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc cctgacaatc
     4081 agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc tgaccgagat
     4141 cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg ccagtcacgc
     4201 ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg atcatcttga
     4261 cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc acaaaccagg
     4321 ttttaccgct atgaacgata ttttccggta catccagtga actgccttca atgccgcgct
     4381 tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg gactcgctca
     4441 acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg ttacggaagt
     4501 caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct tcctgaagat
     4561 caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt tttgatgcga
     4621 agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta tcccttaatt
     4681 gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac ccggagtggt
     4741 tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt agggcaatta
     4801 tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg tgtaccgcaa
     4861 ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga atcgctgacc
     4921 gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct gaaagcatgg
     4981 caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac gccaatactg
     5041 ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca gccattactg
     5101 atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca tgtggcaaac
     5161 ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta tctggcggat
     5221 attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc cgagctgtgg
     5281 cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa ttgcccgaac
     5341 ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt cgcggcagtg
     5401 atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt gatgcaggtg
     5461 attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc gctgcgccac
     5521 accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca tgggattgcc
     5581 gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga atatgagcgc
     5641 atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca ccacgatcaa
     5701 cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg atggcaacct
     5761 ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg tcgaagtggc
     5821 tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg agtgttttcg
     5881 cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc gtatcgtcgc
     5941 ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc cgattggcaa
     6001 tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc aggacaagcg
     6061 cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta cagctatcga
     6121 ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg aagtggcatc
     6181 acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga tgcggcaaac
     6241 gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata ccttcacatt
     6301 gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc cgcgctcaca
     6361 ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca gtttatagaa
     6421 cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct gttcatcgac
     6481 agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt ccacttccac
     6541 cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg cttcctcaac
     6601 cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct tcgatttaac
     6661 cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg tgcgagcaaa
     6721 gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt aagaaatcag
     6781 cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg tcagcgtctg
     6841 actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt aacgttcagc
     6901 cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct gaccgtcgtc
     6961 gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa aacgcacgcg
     7021 gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc caatttcatg
     7081 cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac gcatcaacat
     7141 ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat cttcctgggt
     7201 gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga ggcgatcgtc
     7261 ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg cggattcaat
     7321 atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa aatcatgttt
     7381 gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc gatactgaac
     7441 caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga cttcgagaat
     7501 atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct ggttttcatc
     7561 tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct catcatagtg
     7621 ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc gcacagattg
     7681 gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg caatggttcg
     7741 ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc tgcggctgca
     7801 tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct taccattgcg
     7861 cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata ttgagagtca
     7921 gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg cgctgagcga
     7981 gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag aggcgctgaa
     8041 cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac aacaagccga
     8101 acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc cttttacacc
     8161 ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca ggccaaccag
     8221 gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt cgaaaccatg
     8281 cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta tgccaccggt
     8341 catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt agagtgtatc
     8401 cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga tctgcgtcgc
     8461 ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc tggcgacgct
     8521 ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt gtcggatcgc
     8581 gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg aatgtgcggt
     8641 ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc gcttgagcgt
     8701 attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg tgcattaagc
     8761 gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga gagcatcggc
     8821 cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc aacgttggga
     8881 aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact tctgggtctt
     8941 gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc gctgaaacaa
     9001 ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta catcatccag
     9061 cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat tgccaaatac
     9121 ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta
     9181 ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg
     9241 cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac
     9301 accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca taaaattttg
     9361 accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca cccgttcccg
     9421 tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccatcagt
     9481 gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg caccgtctgt
     9541 gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa tcacgcgggc
     9601 gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat ttccgaaaat
     9661 gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta ctcttcactg
     9721 cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct gctcaaacgt
     9781 accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga gctgggattt
     9841 aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac gctaaagaac
     9901 atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat
     9961 gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga tccctccagc
    10021 ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg
    10081 ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa
    10141 ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga cgtggctatt
    10201 gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata caaacccatt
    10261 gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca tgacgtggca
    10321 attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg tgctgacggt
    10381 caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga aatggtcatt
    10441 atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac
    10501 gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga actgacgccg
    10561 ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggtgatc
    10621 cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct gaacgctacg
    10681 ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga aatggccgcc
    10741 agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggtagcgga
    10801 gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg
    10861 gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc
    10921 gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca ctcctgctat
    10981 gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt aggaaaaacc
    11041 gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc agatcgcggt
    11101 aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg cgcgctggaa
    11161 ggtggcataa atttctttga taccgccaat agctattccg atggtagcag cgaagagatc
    11221 gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc caccaaagtg
    11281 ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt gcgctctatc
    11341 aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat tcatcgctgg
    11401 gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt aaaagccggg
    11461 aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca ggcgctggaa
    11521 ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta taatctgatt
    11581 tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt ggcggtgatt
    11641 ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac taccgcacga
    11701 ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa tgacgcgcag
    11761 atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc acaagttgcg
    11821 ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac ttcgcgggaa
    11881 gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata acagattgcc
    11941 gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga gtccaggcct
    12001 gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag aatacccagc
    12061 ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc gacaatatcg
    12121 tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat cggccacggc
    12181 ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg ccagtcattg
    12241 gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac aatgctgccg
    12301 tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc gatacagatc
    12361 cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag ataccagaaa
    12421 gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc acttccgaat
    12481 ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc gacatcttta
    12541 tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc cgtcacgaat
    12601 aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg gtacgccaag
    12661 gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga aacacaactc
    12721 gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa catggcgaga
    12781 aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt
    12841 cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg aatttgccag
    12901 atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac gtttgatcaa
    12961 gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg ccagcccggc
    13021 ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg ccccagagcg
    13081 cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg ataatggccc
    13141 acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat caaacaaact
    13201 gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg ccagcgtcag
    13261 ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca gtgctttata
    13321 agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca gcgtatcagt
    13381 gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt cgccgatgcc
    13441 cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa tcagggagaa
    13501 ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg gcgatcatgg
    13561 aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg aatcacaggt
    13621 gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc gaacgatttc
    13681 gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg tttagacagt
    13741 tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc cagcaggcgg
    13801 gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac cccggccagc
    13861 agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag gaactggtat
    13921 tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg gacggcacac
    13981 tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat ttcaggcctt
    14041 gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg cacctttgtt
    14101 gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc
    14161 aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc
    14221 agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg caatcaccgc
    14281 gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat aggcaccagg
    14341 cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca gtgcgtcaat
    14401 tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac
    14461 gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc ttcgggttcg
    14521 agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc ttccctttcg
    14581 agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca tgacgtatct
    14641 ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc gagcataatc
    14701 cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca tcgactaaac
    14761 ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg ctgttaatct
    14821 gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta tgctctggaa
    14881 tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta cgcgcgaacc
    14941 aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt tggctatcaa
    15001 tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga gtttgttcat
    15061 ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg gcgctgctgg
    15121 ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc tgaggcgaag
    15181 tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat gcgccaagtt
    15241 ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta ttcaattgct
    15301 cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc agacggtaaa
    15361 gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg cgcgctacgc
    15421 cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta cagggttcga
    15481 gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac gcgtgtactt
    15541 cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg ccatctttga
    15601 caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt tgcgccagct
    15661 ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg cgcgcgatct
    15721 cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa cggatatagg
    15781 cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc ttgctcggta
    15841 cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc atttcaacgt
    15901 aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc aatttctctt
    15961 cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc agctccgcca
    16021 cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg cgtacggatg
    16081 aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc
    16141 aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct gaactgacga
    16201 tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc ttttaccctc
    16261 taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg tttttcgact
    16321 gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg aagtgcgaca
    16381 gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc aagaaacagt
    16441 caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt accttctgcc
    16501 tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta tgtcgctggt
    16561 gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg gtgagtcgaa
    16621 tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg gaacaataga
    16681 caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca gcgtcctcgc
    16741 ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc agttaggctt
    16801 tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga cgattttgtg
    16861 cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg gattgatagt
    16921 aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga
    16981 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    17041 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    17101 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    17161 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    17221 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    17281 cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    17341 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa
    17401 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    17461 caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa
    17521 ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct
    17581 gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata
    17641 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    17701 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    17761 caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa
    17821 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    17881 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    17941 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    18001 agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt
    18061 taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg gcccacaaaa
    18121 gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc
    18181 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt
    18241 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    18301 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac acaacaattc
    18361 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag
    18421 ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc acggatagat
    18481 ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg ggatgatagc
    18541 catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    18601 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    18661 cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    18721 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc tacgtagatg
    18781 gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    18841 ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    18901 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    18961 atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa
    19021 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    19081 aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc tgccgccagt
    19141 cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc
    19201 atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc aatacgcttc
    19261 actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct gcccagcacc
    19321 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    19381 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    19441 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc
    19501 cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg
    19561 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    19621 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    19681 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    19741 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    19801 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    19861 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    19921 acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc
    19981 gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat
    20041 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    20101 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg
    20161 catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    20221 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    20281 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    20341 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    20401 ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc
    20461 gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc
    20521 ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct gaccttcgcg
    20581 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac
    20641 attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct ctttcgcacg
    20701 cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    20761 caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa tgttctgctg
    20821 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    20881 gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    20941 atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt ctttacgaac
    21001 agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga tattttgttc
    21061 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    21121 caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc gcggcgttgc
    21181 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    21241 agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    21301 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    21361 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    21421 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    21481 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    21541 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    21601 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag
    21661 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    21721 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    21781 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    21841 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    21901 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    21961 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    22021 tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag taagcgcgtg
    22081 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    22141 atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    22201 tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct tcaaccaggt
    22261 cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcggacaca
    22321 catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    22381 cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt aaatcttcgt
    22441 aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg tcaaaacgct
    22501 cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg taatcccagt
    22561 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    22621 gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga
    22681 agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc tggaagccgc
    22741 cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg ccgtgcagtt
    22801 tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg
    22861 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    22921 gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc
    22981 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    23041 caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat
    23101 tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg
    23161 ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg
    23221 tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac tggaagcccg
    23281 gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt tttgccgcct
    23341 gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc cggttaccgc
    23401 gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt
    23461 gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt
    23521 gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggctcgt
    23581 caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt
    23641 gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc agcggcatat
    23701 ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg acttcaaaca
    23761 gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc agcaacagtt
    23821 ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga
    23881 gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca aacagacgcg
    23941 ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta
    24001 aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac agtaaacgac
    24061 agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat tcaaaggaga
    24121 aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag tctagtgccg
    24181 gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta
    24241 gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt actggctgat
    24301 ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg
    24361 catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt ggtttcgtag
    24421 tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    24481 ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt atgcccgcga
    24541 gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga acaattactt
    24601 gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    24661 cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga
    24721 cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca
    24781 ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc
    24841 aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca
    24901 ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt gattgccacc
    24961 agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc
    25021 acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg
    25081 tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa
    25141 aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca
    25201 ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc
    25261 gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa ttgttgttga
    25321 tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc gtcaatttgc
    25381 tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa tgtgaagcca
    25441 cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa
    25501 tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt gatcccggca
    25561 acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc
    25621 cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact gtcttccccg
    25681 cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa gctggcatag
    25741 ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg ccagggcgat
    25801 tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca ggcatgggaa
    25861 tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg ctgcgtattg
    25921 tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg gcgataatcc
    25981 tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata cagcgctgtc
    26041 acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc gccgccatag
    26101 aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa gatgatctct
    26161 tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg aagactacgc
    26221 gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg tgggccgata
    26281 tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct gaagccctgc
    26341 ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg ccgccggggt
    26401 tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg cggctgactg
    26461 cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg cagcataatg
    26521 cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc tttgctttgt
    26581 ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc cctgtgatga
    26641 aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa ggatatttgg
    26701 gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc ctcctgaacc
    26761 ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg cggcgtttaa
    26821 acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc gagatacgcg
    26881 tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc ccgccgatgg
    26941 tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct ggcggcaaac
    27001 ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc gtggcgaagg
    27061 ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc agatacaccg
    27121 caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg cgacgcgacg
    27181 ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc atacggccta
    27241 cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca aagttaagaa
    27301 tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg ctaccggcag
    27361 tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg acgtagaaca
    27421 ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca ggatctttcg
    27481 cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc accatttgca
    27541 acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc cacaggttat
    27601 gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg aaaccggcga
    27661 caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc agctcaccga
    27721 agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc agtacccaaa
    27781 tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg gtcacatcgg
    27841 cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa cctgccagcg
    27901 ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta tgtaccgaca
    27961 tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata taggccaaca
    28021 acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa
    28081 ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc tttagcttat
    28141 tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg gcatcgcagc
    28201 cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc tgacgacctg
    28261 ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt aaccacgcca
    28321 gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg ctgaatgcag
    28381 atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc ggagcaatca
    28441 cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt gcgatacacg
    28501 gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt aagccgaggt
    28561 tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa gagagcggta
    28621 cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg accagacagg
    28681 cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt
    28741 aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac
    28801 ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc aggccagccc
    28861 agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa
    28921 caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag ccgttaacga
    28981 agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata gaacccgccg
    29041 gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc ggcgcaagga
    29101 agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata acgatagcga
    29161 aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc ggcgcaatcc
    29221 ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt
    29281 aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg aggctgtcaa
    29341 caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca acggcagtaa
    29401 tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg accattggag
    29461 ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc agagcgatga
    29521 tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa
    29581 cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa caattttagc
    29641 gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg cgttaatgca
    29701 gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa agcaaggagg
    29761 gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa aacactattt
    29821 ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa
    29881 cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg gcaataaaag
    29941 atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat aacaaaactg
    30001 aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg atgattcacc
    30061 gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga acgcgcttta
    30121 tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg
    30181 ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc
    30241 gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc
    30301 tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa tgtctgtttt
    30361 ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc
    30421 ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag caactgcttc
    30481 accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc
    30541 gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac ggtcaatggc
    30601 gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc cagttgatgc
    30661 atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg catgacgcga
    30721 atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt
    30781 ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg cccgttatct
    30841 tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa aataccgccc
    30901 tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg aaagcgttta
    30961 atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg
    31021 tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg atccacccac
    31081 agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc tgttaccgat
    31141 gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag
    31201 caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac gtaagatact
    31261 ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta aaagcgggtt
    31321 gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc
    31381 agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac atacacgtta
    31441 gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg atgcgtcata
    31501 aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc gcccgccatc
    31561 actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc ttccaccgcc
    31621 attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt cggcgaaaat
    31681 ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc
    31741 cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg cttgaggtgt
    31801 ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat taaatccggc
    31861 tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac cggctgaaag
    31921 ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc ttctacgacc
    31981 agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc attaatgcga
    32041 cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg
    32101 ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt ataatcgcga
    32161 ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac tggcatctcg
    32221 gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt gactggctgc
    32281 tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat gttttcgata
    32341 ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc aatttacagc
    32401 aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc acgctgaatg
    32461 aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc ggacatgcgc
    32521 cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc attccgtttt
    32581 tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa aaacagcagc
    32641 atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc tgcaaactgc
    32701 gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg ggttccagta
    32761 aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg gcacaaaact
    32821 ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt attggcggca
    32881 tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa tgcggtaaga
    32941 gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg gaaaacctga
    33001 acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg attaccgcac
    33061 agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat atcgaaatca
    33121 ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc gaatcattgc
    33181 ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc
    33241 aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac
    33301 tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc acgacgttgc
    33361 gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa
    33421 ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca gcaacgggct
    33481 gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg ccatttgtct
    33541 ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa atgatctcat
    33601 gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta
    33661 tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt tgcaggtgcc
    33721 tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag tgaaagataa
    33781 gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc gttcgatgct
    33841 gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt
    33901 gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca
    33961 gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg gcgtcacgtt
    34021 gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta ctgacgaaga
    34081 aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt taacgcgtca
    34141 ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg cgttagccga
    34201 agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg cacgaaatct
    34261 tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca
    34321 gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga gcattcgcca
    34381 ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga atacctggtt
    34441 acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt
    34501 ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt taacccatgc
    34561 tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc
    34621 taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca
    34681 tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta tccagaatgt
    34741 cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc gttataaaga
    34801 aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc gcatcaaaac
    34861 gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt gtggttccac
    34921 cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc agtctcgatt
    34981 actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca
    35041 actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca
    35101 agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca atatcacctt
    35161 gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcaca aacgccagct
    35221 gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata atcagcaaat
    35281 tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg cattggcggg
    35341 ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga cacgtcagca
    35401 agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa accgtattca
    35461 gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga
    35521 aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca
    35581 gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa aagcccaagc
    35641 gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt tccttgcggc
    35701 gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc tggaaaacca
    35761 gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac agcatcaaca
    35821 acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc aagagttagc
    35881 gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc gccagcagct
    35941 gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat
    36001 gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca aagagggcga
    36061 taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc tcgctaatca
    36121 gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg aggcgctgga
    36181 agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta ccctttccgg
    36241 cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc tggtcagcca
    36301 taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg atagcgaaac
    36361 gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt
    36421 gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag tgaaaaagat
    36481 caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac tattcagcag
    36541 gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat cctgcaagct
    36601 atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg tggtatgaaa
    36661 aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga atttggcatt
    36721 atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc tgccgggcat
    36781 atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc actcttttcc
    36841 agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt cattggcaac
    36901 gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct ggtatccggc
    36961 tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat tatcaaaccc
    37021 ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc caatttgctg
    37081 ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt tttattgatc
    37141 gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga tattcgcgac
    37201 gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc cccgtggtta
    37261 attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag ctacgtaaag
    37321 ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat tatgatgtta
    37381 gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg acgttattca
    37441 ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct gatgctcttt
    37501 ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg cgcgggatta
    37561 tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg aggagagtta
    37621 ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg cgcatattgc
    37681 ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc tgccgccctg
    37741 ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg ccagcaagcg
    37801 gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt gcgcgttgct
    37861 tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca cattttgtag
    37921 gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc tacgtttttt
    37981 actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg tgcttcgcct
    38041 tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac tgcccaaccg
    38101 tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca tccggatcga
    38161 gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga cgcaatgcca
    38221 gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct ttcagcgcat
    38281 gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag gtttcaatac
    38341 aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg tcttcgtcca
    38401 tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg atatgcgtcc
    38461 gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca aataccgtct
    38521 gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg gcatcatttg
    38581 ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc tgaccgttga
    38641 gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa cctggaatgc
    38701 ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc aacgtggcga
    38761 tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg cggtacaact
    38821 gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa tcgataccta
    38881 tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg acaaccggat
    38941 tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac gacaaggccg
    39001 tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc cgcgagattt
    39061 cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc ccatgcggca
    39121 gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc gatgcgttaa
    39181 cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa atcctcccgt
    39241 ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg gaacaggcgc
    39301 gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac tctctgctgc
    39361 cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt aacggtttga
    39421 ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta ctgcgtaaaa
    39481 gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa ctgacgctga
    39541 ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat gaagccgagc
    39601 tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat ctgaccagcg
    39661 aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg ctcgactggc
    39721 agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt aagttctgcg
    39781 acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag cgttttgatg
    39841 ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg attgaaggat
    39901 taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg cgacgcctgc
    39961 cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta cggcgagcaa
    40021 taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat cgcctcatcc
    40081 ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg ctggtaaatt
    40141 gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa tcagaaattg
    40201 aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta cgcactccag
    40261 cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc aacgtgatcc
    40321 aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa cgggtacagt
    40381 gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt aacttgctcc
    40441 atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc gaattttccg
    40501 cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt cgacaaataa
    40561 gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca gctgtggatt
    40621 attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg acgccggggt
    40681 atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca gcaatgcacg
    40741 ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa atacctggag
    40801 atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa attgaaaaaa
    40861 tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa aatgccctcc
    40921 cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta ggttcggcaa
    40981 cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct tcatacactt
    41041 gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc gtcatctctt
    41101 ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg ctggcgcgac
    41161 cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac tcattacttt
    41221 gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg ggccagaggc
    41281 gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa tcaatacgtt
    41341 agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac gttggtgaaa
    41401 tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt tcccagcatc
    41461 aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg gcgatttctg
    41521 caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa cgataacccc
    41581 cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg acggctcaag
    41641 aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta ataaaatgac
    41701 gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct ccactttacg
    41761 acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt cttcgcctgc
    41821 ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca ggctatgatg
    41881 ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct gcataggtac
    41941 aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt acttttcttt
    42001 cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg tgctacaaat
    42061 gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa tgattccagt
    42121 aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat gaaaaatgtg
    42181 acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg cggtcactat
    42241 ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt cggatgttca
    42301 ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt taccgtctgt
    42361 cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg cggtttcgtt
    42421 ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct aaaatcaaca
    42481 attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg gttcgtttgt
    42541 tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt ccagcacttc
    42601 ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt cttccggtgc
    42661 agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca cgatcccgtt
    42721 attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg tagcgataac
    42781 ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa atcccgccca
    42841 ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca accactgatc
    42901 ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg ttgttttacc
    42961 acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc cccaataggt
    43021 cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa ctagtacaat
    43081 ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc cttactatac
    43141 caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg actacgttgc
    43201 acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg
    43261 ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa atcgcgcatc
    43321 gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt aagcgccgct
    43381 gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac caaatcgccc
    43441 gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc
    43501 gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag taccagcggc
    43561 atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg acacgcgtcg
    43621 gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat tgtgtcgcgc
    43681 ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa acaatgaatt
    43741 tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg gcaatatggg
    43801 aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc aaatctgggt
    43861 atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca tcaacgccgc
    43921 agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg ttaaacctgg
    43981 catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact ctctggtcgt
    44041 ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa
    44101 aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga cctccgtaac
    44161 gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt tccgctgctt
    44221 tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg tgagcggttc
    44281 ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg tgctgggcgg
    44341 gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat
    44401 ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct caccgggagg
    44461 caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg cagtgatcga
    44521 agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat gactttcgcc
    44581 ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc
    44641 aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag cggcgcaacc
    44701 cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt gtttagccct
    44761 tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga catgattact
    44821 gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt aatttgtaac
    44881 tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg
    44941 ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag
    45001 ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg
    45061 ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc
    45121 tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag ggagagccac
    45181 cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg
    45241 caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc
    45301 aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac gcccattacg
    45361 cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc
    45421 ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg
    45481 accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt
    45541 aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc
    45601 ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct
    45661 tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa
    45721 attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct
    45781 cacccgttag cact
//
LOCUS       DAEBXD010000026        45279 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEBXD010000026 DAEBXD010000000
VERSION     DAEBXD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45279
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.026"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            131..574
                     /locus_tag="KJL94_001439"
     CDS             131..574
                     /locus_tag="KJL94_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4409850.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="KJL94_001440"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="KJL94_001440"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBD4409851.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KJL94_001441"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KJL94_001441"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBD4409852.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KJL94_001442"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KJL94_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBD4409853.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KJL94_001443"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KJL94_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4409854.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KJL94_001444"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KJL94_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBD4409855.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="KJL94_001445"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="KJL94_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBD4409856.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="KJL94_001446"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="KJL94_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBD4409857.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="KJL94_001447"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="KJL94_001447"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBD4409858.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="KJL94_001448"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="KJL94_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBD4409859.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="KJL94_001449"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="KJL94_001449"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBD4409860.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="KJL94_001450"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="KJL94_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBD4409861.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="KJL94_001451"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="KJL94_001451"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBD4409862.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KJL94_001452"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KJL94_001452"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBD4409863.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="KJL94_001453"
     CDS             13934..14314
                     /locus_tag="KJL94_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBD4409864.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="KJL94_001454"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="KJL94_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBD4409865.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KJL94_001455"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KJL94_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBD4409866.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KJL94_001456"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KJL94_001456"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBD4409867.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="KJL94_001457"
     tRNA            complement(17415..17490)
                     /locus_tag="KJL94_001457"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="KJL94_001458"
     tRNA            complement(17494..17569)
                     /locus_tag="KJL94_001458"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="KJL94_001459"
     tRNA            complement(17621..17696)
                     /locus_tag="KJL94_001459"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="KJL94_001460"
     tRNA            complement(17699..17774)
                     /locus_tag="KJL94_001460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="KJL94_001461"
     tRNA            complement(17911..17986)
                     /locus_tag="KJL94_001461"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="KJL94_001462"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="KJL94_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBD4409868.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="KJL94_001463"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="KJL94_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBD4409869.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="KJL94_001464"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="KJL94_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBD4409870.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="KJL94_001465"
     CDS             complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="KJL94_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBD4409871.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="KJL94_001466"
     CDS             complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="KJL94_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBD4409872.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="KJL94_001467"
     CDS             complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="KJL94_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBD4409873.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFVALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="KJL94_001468"
     CDS             complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="KJL94_001468"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD4409874.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="KJL94_001469"
     CDS             complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="KJL94_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBD4409875.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="KJL94_001470"
     CDS             complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="KJL94_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBD4409876.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="KJL94_001471"
     CDS             complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="KJL94_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD4409877.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="KJL94_001472"
     CDS             complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="KJL94_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBD4409878.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27907..30540)
                     /gene="mngB"
                     /locus_tag="KJL94_001473"
                     /pseudo
     CDS             complement(27907..30540)
                     /gene="mngB"
                     /locus_tag="KJL94_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30558..32474)
                     /gene="mngA"
                     /locus_tag="KJL94_001474"
     CDS             complement(30558..32474)
                     /gene="mngA"
                     /locus_tag="KJL94_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBD4409879.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32644..33366
                     /gene="mngR"
                     /locus_tag="KJL94_001475"
     CDS             32644..33366
                     /gene="mngR"
                     /locus_tag="KJL94_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBD4409880.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33469..34338)
                     /gene="sucD"
                     /locus_tag="KJL94_001476"
     CDS             complement(33469..34338)
                     /gene="sucD"
                     /locus_tag="KJL94_001476"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBD4409881.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34338..35504)
                     /gene="sucC"
                     /locus_tag="KJL94_001477"
     CDS             complement(34338..35504)
                     /gene="sucC"
                     /locus_tag="KJL94_001477"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBD4409882.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35598..36815)
                     /gene="odhB"
                     /locus_tag="KJL94_001478"
     CDS             complement(35598..36815)
                     /gene="odhB"
                     /locus_tag="KJL94_001478"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBD4409883.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36830..39631)
                     /gene="sucA"
                     /locus_tag="KJL94_001479"
     CDS             complement(36830..39631)
                     /gene="sucA"
                     /locus_tag="KJL94_001479"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBD4409884.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39932..40648)
                     /gene="sdhB"
                     /locus_tag="KJL94_001480"
     CDS             complement(39932..40648)
                     /gene="sdhB"
                     /locus_tag="KJL94_001480"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBD4409885.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40664..42430)
                     /gene="sdhA"
                     /locus_tag="KJL94_001481"
     CDS             complement(40664..42430)
                     /gene="sdhA"
                     /locus_tag="KJL94_001481"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBD4409886.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42430..42777)
                     /gene="sdhD"
                     /locus_tag="KJL94_001482"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42430..42777)
                     /gene="sdhD"
                     /locus_tag="KJL94_001482"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBD4409887.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42771..43175)
                     /gene="sdhC"
                     /locus_tag="KJL94_001483"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42771..43175)
                     /gene="sdhC"
                     /locus_tag="KJL94_001483"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD4409888.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43556..43663
                     /locus_tag="KJL94_001484"
     CDS             43556..43663
                     /locus_tag="KJL94_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409889.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43869..45152
                     /gene="gltA"
                     /locus_tag="KJL94_001485"
     CDS             43869..45152
                     /gene="gltA"
                     /locus_tag="KJL94_001485"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBD4409890.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT        11165 a        12084 c        11131 g        10899 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatctatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca aacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
    21481 ctgccgcttt cttatcagct gcagcctttt cagcagccgc tttcttctca gcttctgcct
    21541 tggctttttc agcagcttca gttgctgctt tctttcttgc ttcagctgca gctgcttctg
    21601 ccgcttccgc tttcttcttc agtgccgcag cggctacctc ggcttttttc tgcgcttcgg
    21661 ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg
    21721 ctgcttcttc cgcagcttta gcatctgccg ccgctttcgc tgccgccact tcagcttgct
    21781 tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgc tcctgagccg
    21841 ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca
    21901 gttcttcagc agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg
    21961 attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag
    22021 cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac
    22081 tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct
    22141 tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct
    22201 gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa
    22261 ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa
    22321 ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg
    22381 gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc
    22441 acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc
    22501 accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc
    22561 gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg aacaatgttg
    22621 atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg
    22681 ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag
    22741 ttgtcgtaat tcagttccag tttgtttacg cgctggttga gacggttata agccattacc
    22801 gccggaattg cggcgaacag accaattgcc gtcgcaatca acgcttctgc gataccgggc
    22861 gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgacaaaggc gtgcatgatc
    22921 ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac
    22981 ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac
    23041 gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct
    23101 ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctgactc
    23161 tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct
    23221 tcacgcgccg ctgcgttaag aatacgggtt cgctggataa taatggccca ggatgcgatt
    23281 gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa
    23341 tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac
    23401 gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag
    23461 tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta
    23521 tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca
    23581 ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat
    23641 gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca
    23701 caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat
    23761 tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc
    23821 gcgcgattga ggtttgggaa tgagggcaag ttaagggcgc gtaaaaattt atgggcctgg
    23881 agcggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac
    23941 aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg
    24001 acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg
    24061 ccagatttcc agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc
    24121 tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc
    24181 atacaaggtc gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg
    24241 acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga
    24301 atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt
    24361 caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca
    24421 gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg
    24481 aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg
    24541 ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa
    24601 acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcagc agaaccacac
    24661 ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag
    24721 cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt
    24781 tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca
    24841 gtgtcaccag cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg
    24901 tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg
    24961 ccagcaggcc gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat
    25021 attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca
    25081 gcggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt
    25141 cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga
    25201 ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa
    25261 agagtgcgcc gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg
    25321 agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca
    25381 cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc
    25441 accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg
    25501 gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc
    25561 cgaggcgtgc aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc
    25621 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgaa ttcgccacag
    25681 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
    25741 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
    25801 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca
    25861 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
    25921 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
    25981 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
    26041 ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
    26101 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
    26161 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
    26221 cctcttcctg atcaggaatg ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc
    26281 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
    26341 catcacccag aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
    26401 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga
    26461 ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag
    26521 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
    26581 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
    26641 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
    26701 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
    26761 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
    26821 tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac
    26881 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
    26941 agaccgtttc cataatggcc aggaggaacg ccataccgag cgtcagtggc acaaaaagga
    27001 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
    27061 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
    27121 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
    27181 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
    27241 tacgccgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
    27301 ggtgatcaat ttatagctaa attgccgcct ttcagccaat ttgatcgaga acaatttatc
    27361 tctttttgat gcccatttcc aagacttata cattgataaa tatcaatttc tacaaattat
    27421 gttaatcatg tttcaacaag gtaactttag tgatatttat gtttaaaatg tgtgaattat
    27481 aagactgaaa ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca
    27541 cggttgacct taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattat
    27601 taataaaggc ataaatctcc cccccgccga tattaacatt taattaactt ataattatat
    27661 tataattaac tcacccgtgg ttattatttt gttaaaaata aagccacata tacgtagctt
    27721 gagagtgaag ataaacacaa agttacctga tgcgctacgc ttgccagtgt ctacatcatc
    27781 tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc
    27841 ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat
    27901 tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga
    27961 tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca
    28021 agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt
    28081 aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca
    28141 tcccactggg ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt
    28201 gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg
    28261 cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg
    28321 acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg
    28381 cctaggcctt aaaagcagat cttctttccc cagtaagccc acgccgcgca gcaacgtaat
    28441 ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtagccctt cgctaaagac
    28501 agccatgccg ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac
    28561 cggcgcttct ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg
    28621 cgttagcgaa ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat
    28681 caggacgcga aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg
    28741 cctgttgtta tgactaagag tgacaaccaa ctcgacatct actctgccag tggattgtct
    28801 tgcgctgcgt tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct
    28861 ctgccaggcc tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca
    28921 ctcttctttt gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg
    28981 caacacccgg tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc
    29041 attgagcgca atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc
    29101 aatcacgttg ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac
    29161 aatctggttg atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat
    29221 ttgccgatcg attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg
    29281 ctgaccgcga tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat
    29341 cacttcttcc cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg
    29401 cggcatgttg tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc
    29461 agccagttcg aagcgggcga cgatttcgcg atgaactttg tcactacagc agcagccgat
    29521 gctgtcgtgg gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg
    29581 gtatccaaaa cccaacgtcc tggccagcgt tgccagcggt tccagcagat taacaatctt
    29641 attttcaata cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg
    29701 cacgcgcata tatttgccat caataaattc ccctttcagg gttgccagat tatctcgctg
    29761 cgcttcgatc ttctcaaata cctcttcaaa gcggctcatc acaaactttc gttgaggata
    29821 aatttcgcgc agtttctcca tcacttcgaa gatattttgc tgcaatggca tctgatcatg
    29881 cccattcggc agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact
    29941 gtcgaggcgt ttgcgtaatc cgttttcgtc cgcaggtaag tacttaccga tggcataacc
    30001 caacggcatc acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt
    30061 tttatccgta ccgtggtgct cagaacatcc acgccagaac atggtgcagg taatgccaaa
    30121 tccattgtag atatgcggca gttgcccgga catgccaaag gaatcgggta aataacctat
    30181 tttcatcggc tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat
    30241 ggattccgca gaaacaatcg tggtatcggt ctgggtatac taggggccga taatcaactt
    30301 gccggcttct accagtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc
    30361 ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag
    30421 tcggcacagg atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa
    30481 ataccactcc cgatcccagt gcatatgcgg ggtgatgtga acgcgagata ccgctttcat
    30541 cgtcgtttcc tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc
    30601 gcgtgacgcc gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca
    30661 ccaaaccagc caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc
    30721 gagaaaatgc cagcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc
    30781 gttaccatag agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc
    30841 gggatcgccc cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca
    30901 atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc
    30961 gaaaccattt tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg
    31021 cccaggcaga atgcataagc ggctttattc actggccctc caaggtcaaa ggaacacata
    31081 aaaccgagaa tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg
    31141 gtaagcgagt tattgatcca ggcgacaggt tcccccacca caaacagcat cagactgccc
    31201 gctcccaatg taccgagcac cgggtagaga taaaaagtca ggaagccatt gaatttactg
    31261 ctaagacgca agtgattttt catccagcgt atcaagtaac cggctatcaa tccgccaacg
    31321 accgcgccga gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc
    31381 gctaacgccg gtttatctgc cagcgaatag gcggtatagg ctgccagcac cggtaccatc
    31441 aaaattccga gcagcccgcc gcccagtttg cggtacatcc acagccagga gttttcttca
    31501 ttaaacagat cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc
    31561 ccccccgcga caatcaacgg tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc
    31621 tccgttttga cgcttttcac cggttgcgta tcttgttgta aggtacgcgt ctcatcgcca
    31681 cgctcgaggg taagcgattg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca
    31741 ggcacagaaa gcgcaggaat gccgttaaaa cgctcactct ccttgatggc gacttcagcc
    31801 gcaaaaatac aggcagttgc actattgagt tgatccgccg ttaaacgccc ttcaataccg
    31861 ttagcacctt gcttttcaac gtagacattc acgccgagtt tgcgtccggc tttttccaga
    31921 tactccgcag ccatgtaggt gtgagcaata cccgccgggc aggccgttac gcaaacgata
    31981 gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag
    32041 agcaattcat caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgccagg
    32101 cgcgtggtca gcgctgtcag cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc
    32161 gccagcaaca ccactaaatc aaccgcttcc gggccatcca cgccttccca ctgaagcggc
    32221 tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc
    32281 acagccaacc cttcacttaa ggcggtcggg ccaaggcttt cacggcgata cacttctttc
    32341 agaaattgct cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtga
    32401 atggcctctt cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga
    32461 gtcgtcaggt tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga
    32521 caaaacaata ccattgcgta atcaatttca cttgattgaa attatttgca ttttatttgt
    32581 attgtttaca ggatgaattt atcgggcata atggctaaat tcgtattaat gagatgctaa
    32641 ataatgggac acaaaccctt ataccgacag attgctgatc gcattcgtga gcaaattgcg
    32701 cgtggcgagc ttaaacctgg cgatgcgtta cctaccgaat cggcattaca aacagagttt
    32761 ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagt tagtcgagca acagatcctc
    32821 gaaagcattc agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt
    32881 cagttaacca gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt
    32941 ctgatatttg aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgtg
    33001 caggatcgtg tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg
    33061 gaagaaacct ggatgccgct tgcgttgttc ccggatctca cctggcaggt catggaaaat
    33121 tcgaaatatc actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa
    33181 atcattcctc tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag
    33241 ccgattcttg aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc
    33301 cgcaacgctt ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg
    33361 cgataagcac aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg
    33421 ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac
    33481 agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc
    33541 cagagcagcg aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg
    33601 gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt
    33661 aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat
    33721 catcacgatc gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt
    33781 agagccaggg atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc
    33841 cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt
    33901 acccggtttg tgaatgtgac ccggctggat accaattttg cattcacccg gggtgataac
    33961 gcctgggcag ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt
    34021 cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc
    34081 gatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga
    34141 agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt
    34201 agtgccgcct ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg
    34261 ttctgagtgg aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt
    34321 atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc
    34381 gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt
    34441 tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc
    34501 gcgccgatga taccatcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc
    34561 agaacggctt tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct
    34621 ttggttgcgc cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact
    34681 atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc
    34741 agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac
    34801 tggtcacgca tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt
    34861 ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc
    34921 agcgccaggt cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc
    34981 tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc
    35041 gggccagtca gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact
    35101 ttttcgattt ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg
    35161 tctacaacgg caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc
    35221 tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag
    35281 ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta
    35341 ccgcggccac cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa
    35401 gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca
    35461 tagcgggcaa acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct
    35521 tcagtaatcg ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc
    35581 aggtgaagct taaactacta cacgtccagc agcagacgcg tcggctcttc cagtaactct
    35641 ttgatcgtta ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac
    35701 agcgccaggt acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct
    35761 ttgatagcgt gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc
    35821 agggaaccga acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc
    35881 gtcagcttgc catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc
    35941 atgccgaggg tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc
    36001 gccatgctga cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc
    36061 acttccgggt aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc
    36121 agacggatgc cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc
    36181 ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc
    36241 agacgctctg ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca
    36301 gccagagccg gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct
    36361 ttcgccagat gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta
    36421 atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca
    36481 tcgttgtttt gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct
    36541 tcagatttgg cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc
    36601 tgacgagacg ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct
    36661 gatgccggta cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt
    36721 acgactgcgt cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca
    36781 ggcaggtcag ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt
    36841 tcagcgcgtc attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg
    36901 ccggagaggc ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt
    36961 cacggaaatg atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc
    37021 agacaaaatc cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga
    37081 acgggtagag ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct
    37141 gttccagcag gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg
    37201 gatcaagctc gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga
    37261 ggctggaaac cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac
    37321 gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg
    37381 gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg
    37441 aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca
    37501 tccggcccca tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt
    37561 tggcgaagtc accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat
    37621 aaccatattc aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg
    37681 cgccctgacc gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt
    37741 ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa
    37801 ccggaatgcc ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga
    37861 acagtttctc accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact
    37921 gcatttcaac tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca
    37981 tctcaacttt gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga
    38041 aagagtgcat gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat
    38101 cgcggtacag gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca
    38161 gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg
    38221 gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt
    38281 cgatgaagac atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg
    38341 caacggcttc cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga
    38401 tatcagtaca gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct
    38461 ggttgttgat aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt
    38521 tcagagtttc ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg
    38581 gcagcacttt gttgctgttc ggctcatcaa gtctgtccag acgggcacga acagagccga
    38641 taacgaccgg gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca
    38701 ggccgccatc ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc
    38761 cgaggtgttc tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca
    38821 gcacgttcac cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg
    38881 tgccgctgtt gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc
    38941 cttccagcga gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt
    39001 cagcggcggt cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac
    39061 cagactcgat acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact
    39121 cggcaccaat cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca
    39181 tggtttcttt gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg
    39241 tcagatcgtg gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg
    39301 gatctagatt cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt
    39361 taacctgctt cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt
    39421 tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg
    39481 taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta
    39541 agaagtcttc atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt
    39601 ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct
    39661 tacttcgccg tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct
    39721 cttgtctacg tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt
    39781 ttttaaagat ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg
    39841 ggtaatgact tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg
    39901 caacgcttgc gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact
    39961 tgatatggcc gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca
    40021 tgatgctgtg acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag
    40081 tctcggtatc acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga
    40141 taaacttatc gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa
    40201 tacattcata cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag
    40261 ctggcggatt ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga
    40321 attgtcccat gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca
    40381 caatcttctt gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct
    40441 tgccgttcat gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg
    40501 acaggctggg atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac
    40561 cttcttccgc ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat
    40621 agcgataaat tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct
    40681 tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt
    40741 ccgactcagg cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt
    40801 cgaagcggct atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat
    40861 acgccgtttc catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc
    40921 tggaagtgtc atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa
    40981 gccctttcgc catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt
    41041 gcagcgcttt acggatcgcc accggatctt caccgttacg attattgttc caccggttca
    41101 ggcgatccag agacgcttca acatcagact cgctggcatc gtgcagtgcg ccctgctcgg
    41161 cgatagactc ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg
    41221 agttgccgcc cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa
    41281 acagtcccgg aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa
    41341 ctttggtcgg aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt
    41401 tcaccggatc gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt
    41461 cgagaacttc tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat
    41521 cacagccgcg accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg
    41581 ccaggtcttt ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca
    41641 gataaccgcc ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg
    41701 tcgggtggaa ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc
    41761 cgacaccgtc gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgctc
    41821 cgccagtcgc cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc
    41881 acagtgcggt acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc
    41941 actcggagaa aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg
    42001 cgtgaccggt acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt
    42061 tcgactgacc gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca
    42121 ggcccatgtg ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg
    42181 cgtcctggtc accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt
    42241 tatcttcatg ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg
    42301 aacgggtcgg gaagacttta gagagcaggg cacaggtctg gccgctctgg gaaatttgca
    42361 gcgccgcgcg catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg
    42421 gcaatttcat cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc
    42481 aacgacaatc accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa
    42541 cacctgccac atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa
    42601 cactttggtg aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact
    42661 ggtggcgaaa aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg
    42721 aacgaggatg aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg
    42781 aggactcctg cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt
    42841 ttacccgctt cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct
    42901 acgacgacgt gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag
    42961 ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga
    43021 agccactgca ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg
    43081 gacgctatcg ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt
    43141 tttttcacat ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac
    43201 tacgggcaca gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc
    43261 tgcttgcggg tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca
    43321 gatgcgaaaa cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac
    43381 ctggcggcag tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg
    43441 gttttatcct gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc
    43501 taatttgaca atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg
    43561 taaaagttat gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt
    43621 gaccaattca ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag
    43681 tacttgattc gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc
    43741 tgtacccagg ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc
    43801 aggttgatgt gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga
    43861 gaccttaaat ggctgataca aaagcaaaac tcaacctcaa cggggacaca gctgttgaac
    43921 tggatgtgct gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa
    43981 aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta
    44041 cttttattga tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg
    44101 cgaccgattc taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc
    44161 aggaacagta tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga
    44221 ttacccgtct gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta
    44281 ttaccggcgc gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc
    44341 gtgaaattgc cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca
    44401 agtattccat tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact
    44461 tcctgaatat gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac
    44521 gtgctatgga ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca
    44581 ccgtgcgtac cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg
    44641 cttcactgtg gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag
    44701 aaattagctc cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt
    44761 ctttccgcct gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg
    44821 taatgcgtga aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg
    44881 aagtggctat ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac
    44941 tgtacccgaa cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt
    45001 ccatgttcac cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg
    45061 aaatgcacag ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa
    45121 aacgcgactt taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt
    45181 aacccgccgt tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa
    45241 taaaatccac tatcggacat tcatatattc taaaaaaaa
//
LOCUS       DAEBXD010000027        40803 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEBXD010000027 DAEBXD010000000
VERSION     DAEBXD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40803
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.027"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="KJL94_001486"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="KJL94_001486"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBD4409891.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="KJL94_001487"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="KJL94_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBD4409892.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="KJL94_001488"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="KJL94_001488"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD4409893.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="KJL94_001489"
     CDS             complement(3100..6843)
                     /locus_tag="KJL94_001489"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBD4409894.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="KJL94_001490"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="KJL94_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBD4409895.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="KJL94_001491"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="KJL94_001491"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBD4409896.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="KJL94_001492"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="KJL94_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBD4409897.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="KJL94_001493"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="KJL94_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBD4409898.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="KJL94_001494"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="KJL94_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBD4409899.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="KJL94_001495"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="KJL94_001495"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBD4409900.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="KJL94_001496"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="KJL94_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBD4409901.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="KJL94_001497"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="KJL94_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBD4409902.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="KJL94_001498"
     CDS             16240..16347
                     /locus_tag="KJL94_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4409903.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="KJL94_001499"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="KJL94_001499"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBD4409904.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="KJL94_001500"
     CDS             complement(17386..18195)
                     /locus_tag="KJL94_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBD4409905.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="KJL94_001501"
     CDS             complement(18199..18591)
                     /locus_tag="KJL94_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBD4409906.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="KJL94_001502"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="KJL94_001502"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD4409907.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="KJL94_001503"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="KJL94_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBD4409908.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="KJL94_001504"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="KJL94_001504"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBD4409909.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="KJL94_001505"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="KJL94_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBD4409910.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="KJL94_001506"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="KJL94_001506"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBD4409911.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="KJL94_001507"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="KJL94_001507"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBD4409912.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="KJL94_001508"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="KJL94_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBD4409913.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="KJL94_001509"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="KJL94_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBD4409914.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="KJL94_001510"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="KJL94_001510"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD4409915.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="KJL94_001511"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="KJL94_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBD4409916.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="KJL94_001512"
     CDS             29637..32504
                     /locus_tag="KJL94_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4409917.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSPDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="KJL94_001513"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="KJL94_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBD4409918.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="KJL94_001514"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="KJL94_001514"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBD4409919.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="KJL94_001515"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="KJL94_001515"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBD4409920.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="KJL94_001516"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="KJL94_001516"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBD4409921.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="KJL94_001517"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="KJL94_001517"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD4409922.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="KJL94_001518"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="KJL94_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10087 a        10577 c        10175 g         9964 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaagccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt ccagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct ctgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 atg
//
LOCUS       DAEBXD010000028        40250 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEBXD010000028 DAEBXD010000000
VERSION     DAEBXD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40250
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.028"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(7..486)
                     /locus_tag="KJL94_001519"
     CDS             complement(7..486)
                     /locus_tag="KJL94_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBD4409923.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            complement(670..2088)
                     /gene="gltD"
                     /locus_tag="KJL94_001520"
     CDS             complement(670..2088)
                     /gene="gltD"
                     /locus_tag="KJL94_001520"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBD4409924.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="KJL94_001521"
     CDS             complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="KJL94_001521"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBD4409925.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7236..8165
                     /locus_tag="KJL94_001522"
     CDS             7236..8165
                     /locus_tag="KJL94_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBD4409926.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8261..10597
                     /gene="arcB"
                     /locus_tag="KJL94_001523"
     CDS             8261..10597
                     /gene="arcB"
                     /locus_tag="KJL94_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBD4409927.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10827..11480
                     /gene="elbB"
                     /locus_tag="KJL94_001524"
     CDS             10827..11480
                     /gene="elbB"
                     /locus_tag="KJL94_001524"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBD4409928.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11477..12204
                     /gene="mtgA"
                     /locus_tag="KJL94_001525"
                     /pseudo
     CDS             11477..12204
                     /gene="mtgA"
                     /locus_tag="KJL94_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12201..12827)
                     /gene="yrbL"
                     /locus_tag="KJL94_001526"
     CDS             complement(12201..12827)
                     /gene="yrbL"
                     /locus_tag="KJL94_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBD4409929.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13040..13312)
                     /gene="npr"
                     /locus_tag="KJL94_001527"
     CDS             complement(13040..13312)
                     /gene="npr"
                     /locus_tag="KJL94_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBD4409930.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13309..14163)
                     /gene="rapZ"
                     /locus_tag="KJL94_001528"
     CDS             complement(13309..14163)
                     /gene="rapZ"
                     /locus_tag="KJL94_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBD4409931.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14209..14700)
                     /gene="ptsN"
                     /locus_tag="KJL94_001529"
     CDS             complement(14209..14700)
                     /gene="ptsN"
                     /locus_tag="KJL94_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBD4409932.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14818..15105)
                     /gene="hpf"
                     /locus_tag="KJL94_001530"
     CDS             complement(14818..15105)
                     /gene="hpf"
                     /locus_tag="KJL94_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBD4409933.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15128..16561)
                     /gene="rpoN"
                     /locus_tag="KJL94_001531"
     CDS             complement(15128..16561)
                     /gene="rpoN"
                     /locus_tag="KJL94_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBD4409934.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16609..17334)
                     /gene="lptB"
                     /locus_tag="KJL94_001532"
     CDS             complement(16609..17334)
                     /gene="lptB"
                     /locus_tag="KJL94_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBD4409935.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17341..17898)
                     /gene="lptA"
                     /locus_tag="KJL94_001533"
     CDS             complement(17341..17898)
                     /gene="lptA"
                     /locus_tag="KJL94_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBD4409936.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17867..18442)
                     /gene="lptC"
                     /locus_tag="KJL94_001534"
     CDS             complement(17867..18442)
                     /gene="lptC"
                     /locus_tag="KJL94_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBD4409937.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18439..19005)
                     /gene="kdsC"
                     /locus_tag="KJL94_001535"
     CDS             complement(18439..19005)
                     /gene="kdsC"
                     /locus_tag="KJL94_001535"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBD4409938.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19026..20012)
                     /gene="kdsD"
                     /locus_tag="KJL94_001536"
     CDS             complement(19026..20012)
                     /gene="kdsD"
                     /locus_tag="KJL94_001536"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBD4409939.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20026..20991)
                     /locus_tag="KJL94_001537"
     CDS             complement(20026..20991)
                     /locus_tag="KJL94_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBD4409940.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21201..22010
                     /gene="mlaF"
                     /locus_tag="KJL94_001538"
     CDS             21201..22010
                     /gene="mlaF"
                     /locus_tag="KJL94_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBD4409941.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22018..22800
                     /gene="mlaE"
                     /locus_tag="KJL94_001539"
     CDS             22018..22800
                     /gene="mlaE"
                     /locus_tag="KJL94_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBD4409942.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22805..23356
                     /gene="mlaD"
                     /locus_tag="KJL94_001540"
     CDS             22805..23356
                     /gene="mlaD"
                     /locus_tag="KJL94_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBD4409943.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23375..24010
                     /gene="mlaC"
                     /locus_tag="KJL94_001541"
     CDS             23375..24010
                     /gene="mlaC"
                     /locus_tag="KJL94_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBD4409944.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24010..24303
                     /gene="mlaB"
                     /locus_tag="KJL94_001542"
     CDS             24010..24303
                     /gene="mlaB"
                     /locus_tag="KJL94_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBD4409945.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24463..24717
                     /gene="ibaG"
                     /locus_tag="KJL94_001543"
     CDS             24463..24717
                     /gene="ibaG"
                     /locus_tag="KJL94_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBD4409946.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24772..26031
                     /gene="murA"
                     /locus_tag="KJL94_001544"
     CDS             24772..26031
                     /gene="murA"
                     /locus_tag="KJL94_001544"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBD4409947.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26079..26357)
                     /gene="sfsB"
                     /locus_tag="KJL94_001545"
     CDS             complement(26079..26357)
                     /gene="sfsB"
                     /locus_tag="KJL94_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBD4409948.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26584..27555)
                     /gene="ispB"
                     /locus_tag="KJL94_001546"
     CDS             complement(26584..27555)
                     /gene="ispB"
                     /locus_tag="KJL94_001546"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBD4409949.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27813..28124
                     /gene="rplU"
                     /locus_tag="KJL94_001547"
     CDS             27813..28124
                     /gene="rplU"
                     /locus_tag="KJL94_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBD4409950.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            28145..28402
                     /gene="rpmA"
                     /locus_tag="KJL94_001548"
     CDS             28145..28402
                     /gene="rpmA"
                     /locus_tag="KJL94_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBD4409951.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28529..29494
                     /locus_tag="KJL94_001549"
     CDS             28529..29494
                     /locus_tag="KJL94_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4409952.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29510..30682
                     /gene="cgtA"
                     /locus_tag="KJL94_001550"
     CDS             29510..30682
                     /gene="cgtA"
                     /locus_tag="KJL94_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBD4409953.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(30868..32301)
                     /gene="dacB"
                     /locus_tag="KJL94_001551"
     CDS             complement(30868..32301)
                     /gene="dacB"
                     /locus_tag="KJL94_001551"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD4409954.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32549..33025
                     /gene="greA"
                     /locus_tag="KJL94_001552"
     CDS             32549..33025
                     /gene="greA"
                     /locus_tag="KJL94_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBD4409955.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33181..33474)
                     /gene="yhbY"
                     /locus_tag="KJL94_001553"
     CDS             complement(33181..33474)
                     /gene="yhbY"
                     /locus_tag="KJL94_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBD4409956.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33600..34229
                     /gene="rlmE"
                     /locus_tag="KJL94_001554"
     CDS             33600..34229
                     /gene="rlmE"
                     /locus_tag="KJL94_001554"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBD4409957.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34329..36263
                     /gene="ftsH"
                     /locus_tag="KJL94_001555"
     CDS             34329..36263
                     /gene="ftsH"
                     /locus_tag="KJL94_001555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBD4409958.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36353..37201
                     /gene="folP"
                     /locus_tag="KJL94_001556"
     CDS             36353..37201
                     /gene="folP"
                     /locus_tag="KJL94_001556"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD4409959.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            37194..38531
                     /gene="glmM"
                     /locus_tag="KJL94_001557"
     CDS             37194..38531
                     /gene="glmM"
                     /locus_tag="KJL94_001557"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBD4409960.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            38759..39091
                     /gene="secG"
                     /locus_tag="KJL94_001558"
     CDS             38759..39091
                     /gene="secG"
                     /locus_tag="KJL94_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBD4409961.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            39106..39192
                     /locus_tag="KJL94_001559"
     tRNA            39106..39192
                     /locus_tag="KJL94_001559"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39140..39142,aa:Leu,seq:gag)
     gene            39651..40250
                     /locus_tag="KJL94_001560"
                     /pseudo
     CDS             39651..40250
                     /locus_tag="KJL94_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245267.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
BASE COUNT         9470 a         9923 c        10869 g         9988 t
ORIGIN      
        1 cttcgtttat ttgcatgaat atacaaaccg aaaataggag atgcaataca aaagctgtgg
       61 aaatatcaaa cagcctctca atttggcaga cagcgtctgc caaatctttg ccgatgggaa
      121 ttctgcagat ggtgtctgcg gaattcccaa tggtgttaat tcgccagaca ctgtctggcg
      181 aatttcaaca tgttgaaaaa agaggtaaaa aagcctcatt tggcgtaagt tttttgcaga
      241 gaccccacgt ttataccgtc gacttaaatc cttcgatagc cgatcaatca actgtgcacc
      301 ataagctgct ctggcctcac caccttgttc aaattcgaca atccgtcggc caatttccca
      361 gtacgtcgcg gtcatcagcg cgttaacgct acgtaccgtt tccgttcggg cgctatcgac
      421 cagatgaata atgccgttgt ggatttgctg gtagcctgtt gaggttctct ctgagagcga
      481 ttccattatc tgtcccttta tatggcgctg cgttgaataa tggaatcagc tatacgccgg
      541 gcatctaccg gtaacaatcc agatgagcgt attacattgg ttttcttcag attatgcgag
      601 gcgatttcca gaaagaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg
      661 ggacagagct taaacttcca gccagttcat aatgccgtct gccgctttgc gaccttcggc
      721 gatggcggta acgaccagat cggaaccacg gacgatatcg ccgccagcaa agattttcgg
      781 gttgctggtc tggaaagcgt tgtcgttgcc ttccggagcg atgatgcggc cttgtgaatc
      841 cagctcgacg ctgtgttttg ccagccattc catgttgtgt ggacggaaac caaacgccat
      901 gatcaccgca tctgccggaa cgatatgttc ggaacctgca acgatctccg cgcggcgacg
      961 gcctttggca tccggttcgc ccatttcggt acgtaccatt tttacgccgc tgactttgcc
     1021 gttaccgttc acttcaatcc ccagtggctg gacgttgaat ttgaactcta cgccttcttc
     1081 ccgcgcgttt ttcacttcgc ggcgggaacc cggcatgttc tcttcatcac gacgataggc
     1141 acaggtaacg tgctttgccc cctggcgtac agacgtgcgc acgcagtcca tcgcagtgtc
     1201 gccaccgcca agtactacca cgcgtttgcc ttccatgctg acgaacggtt cgtcgcgggt
     1261 ttcaccaaag cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac
     1321 gccatcggcg tcttcgtttt ccagcccgcc gcgcattgac tgataagtcc caacgccaag
     1381 gaacacggca tcgtaatcac tcagcagatc gtccagctgt acgtcgcggc ccacttcggt
     1441 attgagtttg aattcaatac ccatgccggt gaagatttca cggcgacgcg tcattacctc
     1501 tttttccagc ttgaaggccg gaataccgaa ggtcagcagc ccgccaattt ctggatgacg
     1561 gtcgaagaca acggctttta cgccgttacg cgtcaggaca tccgcacacg ccagacctgc
     1621 cgggcctgca ccgataatcg ccactttttt accggtttgc ttcacgccgg acatatccgg
     1681 acgccagccc atttcgaacg ctttatcatt gatataacgc tcaatgttgc cgatggtcac
     1741 cgcaccgaac tcatcgttca gggtgcagga accttcgcac agacggtctt gcgggcagac
     1801 acgtccgcat acttccggca gtgtgttggt ctgatgcgac agttctgccg cttcaaaaat
     1861 acgcccctcg ttggcgagct tcagccagtt tgggatgtag ttgtgtaccg ggcatttcca
     1921 ctcgcagtat gggttgccgc acgacagaca gcggtcagcc tgcgctttgg cctggccttc
     1981 ggaaaacggc tcgtaaattt caacaaactc aattttacgg atcttcagcg gtttctttgg
     2041 cggatcaacg cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc
     2101 ttactgcgcc tgcacgcgca actctgctgc gctacgacta cggtgaccca gcagtgcttt
     2161 tacatcactg gacttcggtt taaccagcgc gaatttcgtg gcgaaggttg accagttcgc
     2221 cagaatctct tcaccgcgct gagaaccggt atgctgaaca tgctcggtga taagaccgcg
     2281 cagatgctct tcatggatcg ccagatcgtc gacgcttaag acttcgacca gttccgggtt
     2341 aacgcgtttg cggaaatcac cgctttcatc gagaacgtaa gcgaagccgc cggtcatgcc
     2401 cgcgccgaag ttaacgccgg ttttacccag aatacagaca ataccgcccg tcatatattc
     2461 acagccgttg tcgccaatgc cttctaccac ggtgatagca ccggagttcc gcacgccgaa
     2521 acgttcaccc gcgcggcctg cggcatacag acgaccaccg gtcgcgccat acaggcaggt
     2581 gttgccgata atgcttgctt catggctgcg gaaggcggaa ccaaccggag gacgaatggc
     2641 gattaagccg cccgccatgc ctttaccgac atagtcgttg gcatccccgg tcaggtacag
     2701 ttccacgccg cccgcgttcc acacaccgaa actctggcct gcggtgccgt tgaagtacgc
     2761 tttgatagga tcggctgcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag
     2821 cgacgcgccg acagaacggt cggtgttgcg gatatcgaac cagaaggttt tgctctggcg
     2881 ttcatcgaca aacggtttcg cctgttgcag caactgcgcg ttcagtaggc cgttatcaaa
     2941 cggcgggttg ttttcagtgc agtagagtga cttacctgca tgtggctcgg cagtctccag
     3001 cagcttcgac agcgccagtt tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag
     3061 gtcggtgcga ccaatcagat ccaccagacg cgttactccc agctgtgcca tcagctcgcg
     3121 ggtttcacgg gcgataaact caaagtaatt agtcaccttg aacggcaggc cgtgatagtg
     3181 attcttacgc agtttgtcat cctgagttgc tacacccgtg gcgcagttgt tcaggtggca
     3241 aatacgcaga tatttacagc cgagcgccac cattgggcca gtgccgaagc cgaagctttc
     3301 tgcgccgaga atcgccgctt tgatgatatc gacacccgtt ttcaggccgc catcaacctg
     3361 caaacgaatt ttatgacgca agccgttggc aaccagcgcc tgttgggttt ccaccagacc
     3421 gagttcccac gggcagcctg catatttcac tgacgacagc gggcttgcgc cggtgccgcc
     3481 gtcatagccc gcgatggtga tcaagtccgc ataagctttc gccacgccag tcgcgatggt
     3541 gccaacgcct ggttcggaca ccagcttaac ggagatcatc gctttcgggt taacctgctt
     3601 gaggtcgaaa ataagctgcg ctaagtcttc gatagagtag atatcgtggt gcggcggcgg
     3661 ggagatcagc gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gagtgacttt
     3721 gtcgcccggc aactgaccac cttcgcccgg cttcgcgccc tgggcgactt taatctgaat
     3781 gacgtcggca ttgaccagat acgccggagt aacgccgaag cgaccagaag ccacctgctt
     3841 gatgcgcgac actttgttag tgccatagcg cgccgggtct tcgccgcctt caccggagtt
     3901 cgagttaccg ccgatgctat tcatcgcttc cgccagcgcc tcgtgggctt ccgggcttaa
     3961 cgcgccgata gacatcgcgg cggtatcaaa gcgtttaaac agttcgcttg ccggttcaac
     4021 atcagcaatg ttgaccgcgt tttcacccgg cgtaattgcc agcagatcgc gcagcgtggt
     4081 tgccggacgc tcattaacca gcttcgcgta ttcctgatag tcgctgtact cgccgctttg
     4141 taccgcctgt tgcagcgtgc gcaccacgtc cgggttgtag gcgtggtatt cgccgccgtg
     4201 gacgtatttc agcaggccgc cctggctaat cggcttgcgc gccagccagg cacgtttcga
     4261 cagattcagc agatcctgct ggaagtcttc aaagcttgcc ccaccaatgc ggctgaccgc
     4321 gccctggaag cacaggccca ctacatcatc gtgtagaccg accgcttcaa acagtttcga
     4381 gcagcggtaa gaggcgatgg tggagatgcc cattttggac atgattttgt acaagccttt
     4441 gttgatgccg ttacggtagt tgagcatcac ggtacgataa tctttggcaa tcgcgtgggt
     4501 gtctaccagg cggcccagcg tttcataggc aaggtacgga taaatagccg tcgcgccgaa
     4561 acccagcaac acggcgaagt ggtgcggatc gcgggcgctg gcggtttcga cgatgatgtt
     4621 ggcatcgcaa cgcaggcttt gatcgaccag acgggtctgg atcgcgccaa ccgccatcgg
     4681 agccggaacc ggcaggcgat ctttagcgat attccggtcg gagagcacca gcaacacggt
     4741 gccgctacgt accatttttt cggctttgtc gcatagctct ttgactgtcg cttcgagcgt
     4801 ggttttagtg acgtcaaagg tgatatccag cgtatctgcg cggtagtgct cctctttcat
     4861 cgtcgtgagc tgtttgaaat cggagtagag cagaatcggc gatttaaagc ttaaacggtg
     4921 cgcctggccc tctgcttcgc aaaagacgtt catttcacga ccgatactgg tggcgagcga
     4981 cataacatgc gcttcacgca gcgggtcgat tggcgggtta gtcacctggg caaactgctg
     5041 gcggaagtag tcgtaaataa tgcgcggctg actggagagc acggcgaatg gggtatcatc
     5101 gcccatcgaa ccgaccgctt cctgaccgtt ttcgcccagt acgcgaatta cggagtccag
     5161 ctcttccgcg ctgtagttaa actgtttctg gtagctggca agcgtgtcgt cgtccagttc
     5221 gcggctaccc acttcttcat cgggcagatc ttcaaacggt accagtcggc ggacgttttt
     5281 ctccatccac tctttataag ggtggcggct tttcagatcg tcatcggttt ctgccgagtg
     5341 cagaatacgc ccactgcgag tgtcgatcac catcagttcg cctggcccga cgcggccttt
     5401 ttcgaccact tcgtcaggct ggtaatccca gataccgact tcagaggcgc aggtgatgag
     5461 cttgtctttg gtaatgacgt agcgtgccgg acgcagaccg ttacggtcga ggttacaggc
     5521 agcgaagcga ccatcggaca tcacgatgcc cgccggacca tcccacggct ccatatgcat
     5581 ggagttaaag tcaaagaagg cacgcagttc cgggtccata tccgggttgt tctgccaggc
     5641 gggtggcact aacaggcgca tggcgcggat gatatccatc ccgcctgcca gcagcagttc
     5701 cagcatgtta tccatggaac tggagtcaga accggtttcg ttgacgaatg gcgcggcgtc
     5761 gtgcaggtca gggatcagcg gcgtctggaa tttataggta cgcgcacgcg cccattggcg
     5821 gttaccggtg atggtgttga tttcaccgtt atgcgccaga tagcggaacg gttgcgccag
     5881 cggccagcgc ggtacggtgt tagtggagaa gcgctggtgg aacaggcaaa tggccgattc
     5941 cagacgcagg tccgcaagat ccagataaaa gcgcggcaga tccgccggca tacacagacc
     6001 tttatagatg ttcaccagat tcgacaggct acagacgtag aagtctttgt cggcttcgag
     6061 acgcttttca atgcggcggc gggcgataaa cagacggcgc tccatatcgc gtggacgcca
     6121 gcctgccggg gcgttcacaa aaatttgctc aatgcgtggc agagaggaga gggcgatttc
     6181 acccagcacg ccttcgttag tggggacatc acgccagccc acaatcgaca aggtttcgcg
     6241 ttgcagttct tcttcaacga tgcggcgtgc ggcagcggcg agttcaggat ctttattcag
     6301 gaagagcatc ccgacagcgt agttttttgc taaacgccag ccgcgctcct gcgcaacgat
     6361 gcgaaaaaag cgatccggtt tttgtaacag caagccgcaa ccgtcgccgg ttttaccatc
     6421 ggcgagaatc gcgccacggt gctgcatgcg ggccagtgcg tgtattgcag tacgcactac
     6481 cttgtggcta ggttcgcctt ctatgtgggc gatcaggccg aaaccacagt tatccctctc
     6541 aagggattta tcgtacaaca tatcgtgaac ctcccccagg ctctgcggga accccacttc
     6601 cgaaccgctg cgcacgggca cagaaagagc gtggcgacgg ggtttgcgtg tcatacgcgc
     6661 gcctcgcatt cgcccccttg ttgtcctttt cgcatcggtt aatacggtga agaggggatc
     6721 tcaattactg cataaatatg atgagcaggc tgctcatcca gaaagcttcc agcggatttc
     6781 caacttatcg ggaaagctga tacaggtcaa atggcaagct tattggtaca gaaatgtgct
     6841 ttagcgcaaa attctttatt aaatagctga aattaaaata gattttatct ttaaggctaa
     6901 aaggagagtc gttagagaaa cagtcaattc ctgactgtca taaaggcgtg ttaaccctta
     6961 tcctgccttg aactgacgag aaaaccagca ttttatactg ccttaattgg taatgacgtt
     7021 tcatgctact gtttttgcct aaaatccata aggagataaa aaaatctgac acaagataag
     7081 caaaagtgat ttatgtgatt gtgcatgaaa tttcggtaat tttgattgat tatgcagttg
     7141 ataaaagcag gccgggacga ttgacccagg tcatcgccga attgagctaa aaatggcagg
     7201 ctttggcctc ttttcaaggc gcggtctatc agattatgca gttacagaaa ttagtcaata
     7261 tgtttggtgg tgatctcacc cgtcgttatg ggcaaaaggt gcataagctg acgctgcacg
     7321 gcggttttag ctgccctaac cgtgacggta ccatcgggcg tggtggctgc acattctgta
     7381 atgttgcctc gtttgccgat gaagcgcagc agcatcgttc cattgccgag caactggcgc
     7441 accaggcgaa tttagttaac cgcgctaaac gctatctggc ctactttcag gcgtatacca
     7501 gcacctttgc ggaagttcag gtgctgcgtt ctatgtatca gcaggcggtg agccaggcca
     7561 atattgtcgg tttgtgtgtt ggtacccgcc cggactgcgt gccggatgcg gtgctggatc
     7621 tgctttgcga atataaggac cagggttacg aagtgtggct ggagctgggg ctacaaaccg
     7681 cccacgacaa aacactgcat cgcatcaacc gcggccatga ttttgcctgt tatcagcgta
     7741 caacccagct ggcacgtcag cgcgggctga aggtgtgttc ccacttaatt gtcggcctgc
     7801 ctggcgaagg gcaggctgaa tgtttgcaaa cgctggagcg agtggttgaa accagcgtgg
     7861 acggcataaa gctgcatccg ctgcatattg tgaaaggcag cattatggcg aaagcctggg
     7921 aagcggtgcg tttgaacggt attgaactgg aggattatac gctcactgcc ggagaaatga
     7981 ttcgccatac gccaccggaa gtgatctacc accgcatttc cgccagcgcc cgtcgcccga
     8041 cgctgcttgc tccgctgtgg tgcgaaaatc gatggacggg aatggtcgag ctggatcgct
     8101 atctgaatga acatggcgta cagggatcgg cgctgggacg tccgtggcta cctccaacgg
     8161 agtaggtcgt tgaggggaat tccgcatttc tcacacaatt tataacgtaa ctgtcagaat
     8221 tgggtattat tggggcaggt tgtcgtgaag gaattcccta atgaagcaaa ttcgtctgct
     8281 ggcgcagtat tatgttgacc tgatgatgaa gttaggtctg gtgcgcttct caatgttgct
     8341 ggcgctggcc ctcgtcgttc ttgccattgt ggtacaaatg gcggtaacca tggtgctgca
     8401 tggtcaggtc gaaagcattg atgttattcg ttctatcttc tttggtttgc tgattacgcc
     8461 gtgggcggtc tactttctat cggtggtcgt cgagcaactg gaggagtcac gacaacgtct
     8521 gtcacggctg gtgcaaaaac tggaggagat gcgcgagcgc gatttgagcc tcaacgttca
     8581 gttaaaagat aatattgccc agctaaatca ggaaattgcc gttcgtgaaa aagcggaagc
     8641 agaactgcag gaaaccttcg gccaactgaa aattgaaatc aaagagcgcg aagagacaca
     8701 aattcagctc gagcagcaat cctcattctt acgttccttc cttgatgctt cacccgactt
     8761 ggttttttat cgtaacgaag ataaagagtt ttccggctgt aaccgcgcga tggagctgct
     8821 gaccggaaaa agcgaaaaac aactggttca cctgaaacct gctgatgttt actcaccgga
     8881 agccgccgca aaagtcattg aaaccgatga aaaagtgttc cgtcataatg tgtcactgac
     8941 ctatgaacag tggctggatt acccggacgg gcgcaaagcc tgctttgaaa tccgtaaagt
     9001 gccgtactac gaccgcgtgg gtaaacgtca cggtttgatg ggctttggtc gcgacattac
     9061 cgagcgtaag cggtatcagg atgcgcttga acgcgccagc cgcgacaaaa cgacgtttat
     9121 ttccaccatc agtcacgaat tgcgtacacc gctgaacggt atcgtcggcc tgagccgcat
     9181 tctgctggat accgaactca ccgccgagca ggaaaaatat ctcaaaacta tccatgtttc
     9241 ggccgtcacg ctggggaata tctttaacga tattatcgac atggataaga tggaacggcg
     9301 caaggtccag cttgataatc agccggttga tttcaccagc ttccttgccg atctggaaaa
     9361 tctctccgca ttgcaggcgc aacaaaaagg attgcgcttt aacctggagc ctacgctgcc
     9421 attaccgcat caggtcatta ccgacgggac gcgtttacgg cagatcctgt ggaacctcat
     9481 cagtaacgcc gtcaaattca cccagcaagg ccaggttacc gtgcgcgtgc gctacgatga
     9541 aggcgatatg ctgcattttg aagtggaaga ttctggtatc ggcattccgc aggatgagct
     9601 ggataaaatc ttcgccatgt attaccaggt gaaagacagt catggcggta aacctgccac
     9661 cggcaccggt attggtctgg ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat
     9721 tacggttacc agtgaacagg gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc
     9781 ggtggcagaa gaggtcgatg atgcgtttga tgaagacgat atgcctttac cggcgctgaa
     9841 tgtgctgctg gtggaagaca ttgaactgaa cgtgattgtc gcgcgttctg tgctggaaaa
     9901 attaggtaac agcgttgatg tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc
     9961 gggcgaatac gacctggtgt tgctggatat tcagttgcca gatatgaccg ggctggatat
    10021 ctctcgtgaa ctgacgaagc gttatccgcg cgaggattta ccgccgctgg tggccttaac
    10081 cgctaacgtg ctgaaagaca aacaagagta cctcaatgct ggaatggatg atgtgctgag
    10141 taagccgctt tcggttccgg cgctaaccgc gatgatcaag aaattctggg atacccagga
    10201 tgatgaggag agtacggtga cgacagaaga gaacagtaaa tcagaagcat tgctcgatat
    10261 tcccatgctg gaacagtatc tcgaacttgt aggaccgaag ctgatcaccg acgggttagc
    10321 ggtgtttgag aagatgatgc cgggctatgt cagcgtgctg gagttgaatc tgacggcgca
    10381 ggataaaaaa ggcattgttg aggaaggaca taaaattaaa ggtgcggcgg ggtcagtggg
    10441 gttacgccat ctgcaacagc tgggtcagca aattcagtct cctgaccttc ccgcctggga
    10501 agataacgtc ggtgaatgga ttgaagagat gaaagaagag tggcgtcacg acgtagaagt
    10561 gctgaaagcg tgggtggcaa aagccactaa aaaatgaccc cggctagacc ggggtgcgcg
    10621 aatactgcgc caacaccagg gaaatcttgg ctgcgccgta aattattatg atgagttaca
    10681 agggcacagc actgtttttc aggccgcacg cagttaagat agcaaaactt aaatgatttg
    10741 ttacatgaat cagttaaatg tgtgatgcgt accattttta ttaaaaaaat taatgtgttt
    10801 cagtcaattg cgaaaggatg atcacaatga agaaaattgg cgtaattctg agcggatgcg
    10861 gcgtctatga cggttctgaa attcatgaag cggtgttgac gttgttagct atttcacgca
    10921 gcggtgcgca ggcggtctgc tttgcaccgg ataagcagca ggttgatgtt atcaaccatt
    10981 taactggcga agcgatgacg gaaacgcgca atgtgctgat tgaagcggca cgaataacgc
    11041 gcggtgaaat ccgtcctctg gcccaggccg atgccgctga actggatgcg ttgattgtgc
    11101 cggggggggt tggcgcggcg aagaatttaa gcaattttgc cagtcttggt agcgaatgca
    11161 ccgttgaccg tgaattaaag gcgctggcac aagcgatgca tcaggccgga aaaccgcttg
    11221 gttttatgtg tattgccccg gcgatgctgc cgaaaatttt cgatttcccg ctgcgtttga
    11281 ccatcggtac tgatatcgat accgcagaag tgctggaaga gatgggcgcg gagcatgtgc
    11341 cgtgtcctgt cgatgatatc gtggttgatg aagacaataa gattgtcacc accccagcat
    11401 atatgctggc gcagaacatt gcagaagcgg cgagcggcat tgataagctg gtttcccgcg
    11461 tgctggttct ggctgaatga gtaaaagccg cttaacggtg tttagtttcg ttcgccgttt
    11521 tcttttgcgg ttaatggttg tcctcgccgt tttctggggc gggggcatcg cgttgtttag
    11581 cgttgcgcct gttcccttct cagcggtaat ggtcgagcga caggtcagcg cctggctgca
    11641 tggcaatttt cgttacgtgg cacattctga ctgggtcagt atggatcaaa tctcgccgtg
    11701 gatgggactg gcggtgattg ccgcagaaga tcagaaattt cctgagcact ggggctttga
    11761 tgtcgcttcc attgagaaag ccctggcgca caacgagcgc aatgaaaacc gtattcgcgg
    11821 tgcttcaacg atttctcagc agacggccaa aatctctttt tatgggatgg gcgtagctgg
    11881 gtacgaaaag ggctggaagc tggattaacg ctggggatag aaacagtctg gagcaaaaag
    11941 cgtatcctga cggtttacct gaatatcgcc gaatttggcg acggtgtgtt tggcgtcgaa
    12001 gctgcggcac aacgttattt ccacaaaccc gcgagcaaac ttacccggtc ggaagctgca
    12061 ttactggcag ctgtattacc taatccactt cgtttcaaag tctcctcgcc atcgggctac
    12121 gtgcgtagcc gtcaggcgtg gattttacgg cagatgtatc agttaggcgg tgagccgttt
    12181 atgcaacaac accagctgga ttaagcttcg cgcgaagtga gggccagcgt tttgctctca
    12241 cgaggttgca gatctttctg cagggcaata gccagcgccg gttgggcgat aaatcgtttc
    12301 cataaccgct cttgcttacg cagacaacac cacttcgacc aggtcgccag tggaatcaat
    12361 gtgctttcac caatgttgtc gcagacgacc gggataacct ctgactcact gatgcggtga
    12421 cagaggatat tctgcggctt cagcgacatc gtcacgatac ggttatcctg caaataacgc
    12481 ttcagctgtt tcagtagctg gcgcagttgt gcaatgtctt cttcgtaacg gcattgttca
    12541 gcaaattcgg tcagcgtaat ggaaggcttg ccgtcaaaat cggcaatcac atcgtagaca
    12601 taaccggttc cgcaatccgt ctcaacggta ccgtgatagc gcggtattcc actccagtct
    12661 tttaaccggc gaccaagatg cgcgtagtac tttaactcac ggcggatctc tttatcgcca
    12721 ccacgatggt agacaatctt gatacagcgt tgggcatctt ccggatgcgc ataacatttc
    12781 cgatgtcttc cggtgcccag gggactttgt tcagataagc gaatcatgcc atctcctgga
    12841 aatattttct gacgatctcc agtatggtcg attaacttaa acaaaaccta aacaatgcct
    12901 cttaaggttt tcttaaggtt cttctgaaag tgaaaagcgc acaaaatgta caaatgacgt
    12961 tgattgagaa cgatttcagt acgtgatttt cttattgtcg ggggagcttg aagggagttg
    13021 tatgtcaaag tgatgaagat taatcttcat caaaaccaga attaaagagg gcgataacgg
    13081 cggccagtgc ttcctcttcc tgtggaccgg tcgcttcaac ttcaatctgc cgtcctttgg
    13141 cagaatccaa catcagcagc gcaataacgc tgttggcttc agcctcggtg ccttcgtcat
    13201 tacgtaacag cacttcagcg tcaaaaccct gcattaattc aaacagcttc attgcaggcc
    13261 gggcatgcat gcccagcttg tttgtgattt caacagtttg cttgacggtc atggtttacg
    13321 tttttccagc gtacgatggc gtgactggac gtttttaccg cgcgagcgga agtagtctgc
    13381 cagttgctct gcaatataca ccgaacggtg cttcccgccg gtacaaccaa tggcgaccgt
    13441 caggtagcta cggttgttgg tttccagcat aggtaaccat agctcaagat agcttcgcgt
    13501 ctggtagata aaattgtgta cttctgtgtg gcggtcgagg aacgcggcga caggtttatc
    13561 aagacctgtc attggacgca gtttcggatc ccagtgcggg ttcggcaaga agcgcacgtc
    13621 aaagacgtaa tctgcatcga tagggatacc gtgtttgaag ccgaaagact caaagaccat
    13681 ggtcagttcg cgttcacgtt tacccagcag acgggtacgc agcatttcag ccagctcgtg
    13741 aacggacatt tctgaggtat cgacaatcag atccgctcgc gaacgcagag gctccagcaa
    13801 atcgctttct ttgtcgatag cactttccag cgacaggttt ttgctggaaa gcggatgcag
    13861 tcggcgcgtg tcactgtaac gacgaattaa ggtattacgg tcggcatcca ggaacagtag
    13921 ttgcggtgag aaagcgtcag gcaggttact catcgcctgt tcgaatattt ctggtgactc
    13981 cggcatatta cgaacatcaa tgctgacggc ggcagaaatc tcgcgatcgg ccagagttcg
    14041 agccagatcg ggtaacaaca ctacgggaag gttatccacg cagtaaaaac ccatatcttc
    14101 cagcgcacgc agggcgacag atttacctga acctgaacgt ccgctgacga tcatcagtac
    14161 catgtactgt ttctcctcac aacgtctaaa agagacatta ccgaataact acgcttcatc
    14221 cggagtacct tcggtatccg taatgatttg atacagctct tcatcgctct gggctgcgcg
    14281 taaacggcgg cagatggttt tgtccgccag acgtttcgcc accagcgaca gagtatgcag
    14341 gtgcgtttta gtttggtctg ccggcaccag cagggcaaaa agtaagtcca ccggttggtt
    14401 gtcgatggca tcgaaagcta taggcgtttc gagctgaacg aaaacgccaa cggcacgcag
    14461 agtatcttct tcaagtttgc catgcggaat ggcaatacca ttgccgatac cggtactgcc
    14521 cattttttcc cgcgtcagga tagcttcaaa aaccacctga ggcggcaggc taagttgttt
    14581 cgccgccagc tcgctgatga tttccagggc gcgttttttg ctctgacagt ggacgcggct
    14641 tcgcgtacat tccctgttaa gaacactgct aagctgtaga gttgtatcat tatttgtcat
    14701 aatttcacct aagaacctgc ccactcaaac agtggaacag gctcggagca ttaaccgtac
    14761 aaatggcccg ttgtgccgca caacaggccg ccatgcacat gctaattgcc cggacaatta
    14821 gtgttgtttc agtttatctt tatgtttggt caactggcgt gccagcttat caattaagcc
    14881 atcaatggca gcgtacatgt cctgaccttc cgcgctggca tgaatttcac caccgtttac
    14941 atgcagtgtt gcatctgagg tgtgggtgac tttctccact ttcagaacaa catagacctg
    15001 gttgattcgg tcaaaatatt gctcaagttt ggcaaattta gctgtaacaa attcgcgcag
    15061 tgcctcggtg atctcgacgt tatttccggt aatgttgagc tgcatagtgt cttccttatc
    15121 ggttgggtca aacgagctgt ttacgctggt ttgacggcgg aatggataaa gactctcggt
    15181 acttcgcaac agtgcggcgt gccaccatga taccttgttc cgacagcaaa gaggttaact
    15241 tgctgtcgct caacggtttc gctgggtttt ccgccgcgat taatttcttc accagtgcac
    15301 gaatcgccgt ggaggaagct tcgccgccgc cctcggtatt gacgtgactg gaaaagaaat
    15361 acttcagttc aaaaatgcct cgtggactat gcaggtattt ttgcgtggtc acgcgagata
    15421 tcgtcgattc atgcatttcg acagcctggg cgatatcggc cagtaccatc ggtttcatat
    15481 attcttcacc ttgctcaaag aaggcttgct gctgttcaac gatacagcga ctcacgcgca
    15541 gtagcgtatc gttacggctt tccagactct tgatcaacca tttggcatcc tgcagattgc
    15601 tgcggataaa ctggctgtca ccatcgttgc gcgcgttatt gcacatcgag gcgtagtgct
    15661 ggttgatttg cagacgcgga atgctgtcac tgttgagttc taccgtccag tgaccgttat
    15721 gcttacgcac cagcacatct ggaatgacat actcaggttc gccagtctgg atcgactgcc
    15781 cggggcgcgg atcgagcgac tggatcagat tgacggcttc tttcagcaca tcttctttca
    15841 gacgcgtgac gcgcattaaa gtgcggaagt cgtgattggc taacagatcg agatgatcgc
    15901 taatgatcaa tctggcctct tccagccacg gcgtggtctt atcgaattgg gagagttgga
    15961 ttagcaggca gtcacgcaga tcttttgccg ctacaccgac cgggtcaaac cgttggatcc
    16021 gcttaaggac ggcttcaacc tcgtcgatgt cgatctcttc atcgcccata ctttcgagaa
    16081 tatcttccag cgggacagtc agataaccgg tttcatcaac ggcatcgacg atagaggtag
    16141 caatagcgcg gtcagtgtcg gaaaacggtg tcagctcgac ctgccacatc aggtaatcct
    16201 gcaaggtctg cgtcgtttcg ccctggtaga ccggcagctc gtcgtcaatg tagtcaccgc
    16261 tggtgccgga tggtgtacca gcggtgtaaa tggtgtccca actggcatcg agcggcagct
    16321 cttccggcat ctctttttgt tcgagcgcgt cggcggtgtc cagcgtttca ctgtcttgcg
    16381 tttcgcgggt gtcgatttct tcatgagtgt cgatttgctc aagcagcgga ttactctcca
    16441 gcgcctgctg tagctcctgc tgaagttcca gcgtcgacaa ctgcaacaga cgaattgcct
    16501 gttggagctg tggcgtcatc gccagttgtt ggctaagcct gagttgcaaa ccttgcttca
    16561 tgttcagaat cgtactctcc tgctaaaacg tcgcaaactt ctaccctatc agagtctgaa
    16621 gtcttcccca aggtatacac gcttaacgtg ttcgtcttgt aagatttctg taggcgtgcc
    16681 gtgggagatc aaatgcccct gactgacgat ataagcgcgt tcacaaaccg ccagtgtttc
    16741 acgcacgttg tggtcagtga tcagcacgcc caggccgctg tcgcgcaggt gttcaatgat
    16801 gcgtttaatg tcgataaccg agatcgggtc aaccccggca aacggttcgt cgagcagaat
    16861 aaatttcgga ttcgcagcca gtgcgcgggc aatttctaca cggcgacgtt cacccccgga
    16921 gagtgactgc cccatgctgt cacgcaggtg ctcaatgtga aactcttcca tcagctcgtt
    16981 cgcgcggtct tcacgttgtt cagcagacaa gtcgtcacga atttgcagta ccgccatcag
    17041 gttatcgtaa acgctgaggc gacggaaaat ggaggcttcc tgtggcagat agccgatacc
    17101 gcggcgcgcg cgtgcatgca gaggcagcag actgatatcg tcatcatcaa taatgatgtt
    17161 gcccgcatcg cgcggcacaa tgcctacaac catgtagaaa gtggtggtct taccggcacc
    17221 gtttggcccc agcagaccga caatttcccc ggagttgacg gtcaggctga cgtcttctac
    17281 cacgcgacgg cctttatagg cttttgcaag gttctttgca gttaatgttg ccataacgaa
    17341 ttaattaccc ttcttctgtg ccggggtctg gcctttgttg tttttgtcct gcagctgcga
    17401 cggcaccaga acggtggtta cgcgcttgcc tttgtcgctg aaagcctgca ttttctgctc
    17461 tttcaccagg taagtgatct tatcgccctt aatgttgcta tcgacctgct gcagataagc
    17521 attacccgtc agaacgacaa aatcttttgc cagttcgtag tgcatctggg aagcgtgacc
    17581 ttcaacgggt ttaccgttgt cctgcatctg gtagaacgtt gccggtttac cgtatccgtc
    17641 aatcacttct ttaccttgtt cgccgcccgg acgggtaacg accactttgt cggcattaat
    17701 tttgatggtg ccctgggtga cgatgacatt accggtaaag gtaaccacgt tgccttgcat
    17761 atcaagagat tgctggtccg attcaatgtg gatcggctga tcagtgtctc cggttacggc
    17821 aaatgccgga atgctggcgg ccagaagtga gctggcaagc acaagattaa ggctgagttt
    17881 gtttgttttg aatttcatag gatgttctaa ccttttcaat cagctcggcg ttcttgctgc
    17941 gtaagttgcc gcgcattttc agaccgctgg agttaaatgt tgttccgtat aacgtgacga
    18001 ggtcttcaga ggtaacatcc tgcgtcacca gattgatctg cgcgttatcc gtcgtgattc
    18061 tgcgaagttg agagtctggc acaagtgcgt tgacttcaac gtgtccatat aaatagagca
    18121 tccggtcatt ggtcagcttg gctttatctg cttttacgga ccatgtcggg attttatcct
    18181 tatcaaacgt ggtaagtacc ggctgcgtaa accacgaaac ggcctgatcg gaataatatt
    18241 caacgtgttg agcaatcaat cgatagctta gtgccccttc tgggttatag acgagcgtgt
    18301 ccgtatgctc gcttttatag gtgggatcat tgttgttgac gaccacctgg gcggtatcgt
    18361 ctttttcggc catattaatg ccgatcatca ccagaaccgc cagtgatagc acaatgataa
    18421 cccaacgtct ggctttactc atatcgattg ccctttggct tcatccagtt tgccctgcgc
    18481 caggagtaat aagtcgcaaa cttcgcgcac tgcgccacga ccgccagcaa tgcgcgtcac
    18541 gtaatcggcg cgcgggatca acagtggatg cgcatcggcc acggcgacgc ttaaacccac
    18601 tttttccatt accggccagt cgatgagatc atcgccaaca taagccacat tttccggggc
    18661 aatcgccaat ttttccagca aatcgctaaa ggcgatcagt ttgtttgact gcccctgata
    18721 caagtgagtg atccccaatg tggcacaacg atcttctacc agtttagcct ttcgcccggt
    18781 aatgatagcg acttcaatat cagaggtgag cgcacaacga atgccataac cgtcacgaac
    18841 attgaacgct ttcagctctt cgccgttatt gcccatataa atcaggccat ctgacagtac
    18901 gccatcaacg tcgaggatca gcagacgaat gttctctgct tttgccataa cgtcggcgct
    18961 gacagggccg taacaggtcg caagcgacgc acctgctttg ctcattgttg tttatccttg
    19021 aatctttaca ctacgcctgc acgcagtaaa tcatgcatat gtaacacacc gagtaaatgg
    19081 tcgccatcgg caaccatcac ggaggtgata tggcgggact gcattaagtt cagtgcctca
    19141 acggccagaa tgccagggcg cacacgtatt ccccccggcg tcatcacatc ggcaatactt
    19201 aactgacgaa catccacgcc catatcgaag acacggcgta aatcaccgtc ggtaaagatg
    19261 ccttcaatca tcatattgtc atcgcaaatg acagtcatac caagattttt gcgggtaact
    19321 tccagcaatg cgtcacgcag actggccgtt ttcttaacat gcgggatctc atcgcccgta
    19381 tgcataatat cgtttacgcg cagcagaagt ttacgaccca gtgcgccgcc tgggtgtgag
    19441 agcgcaaaat cttcagcagt aaagccgcgt gcttttaaca gcgcgacagc gagggcatcg
    19501 cccataacca gcgtggcggt ggtgctgctg gtcggtgcca gccctaacgg acaggcttct
    19561 ttcgctactt taacacacag atgcacatct gcggcgcgcg ccatgctgct ctccgggcga
    19621 ccggtgatgc agattaacgg tacgtgaaga cgcttaagca ctggaattaa ggccgtgatt
    19681 tcgctggatt cacctgagtt agagatagca atcaccacat cctgtggggt gaccatgcct
    19741 aaatcaccat gcgcggcttc actaggatgg acgaaaaatg aaggtgtacc ggtgctggca
    19801 aacgttgcgg ccattttgcg cccgatgtgc cccgatttcc ccatccccat gacgacaact
    19861 ttccctttac accagaacat cttttcacag gcaagcgtga aattctgatt gatgtattga
    19921 tcaagctccg ccaggcattc acgttcaatc gccaggactt ctttacctgc ttgctgaaag
    19981 tcaaaacccg gttgtaactc tacgtgcgac ataatgcgtt tccagttatt caacgagtat
    20041 tggcgataac cagtacaaca tcgccagcca tacgataaat ccgccagtta ataatacccc
    20101 tacaccacgg cccggttgcg gggagcgccg ccagcacagc aacgcaaaaa taatgctcac
    20161 cagcaacatc acgctgtagt cacgactgta cgccagtgga tcaatctctc ctggcgttat
    20221 cagcgcgggt aaacccaaca cgatgacaat attaaaaatg tttgcgccaa tgatatttcc
    20281 gacagcaatg tcgttttcac ctttgcgaac ccccgctatt gcggttgcca gttccggcag
    20341 actggttccg atagcaattg ccgtcagagc catcgtcaac tcgctgatgg caaagtaatt
    20401 cgccagcacc gtggcgttat caaccaccat ccgcgtggcc actggcatga tgataagcgc
    20461 aatgccgagc cataaaaacg cgacgggcaa tccgccgtca cgcggcagct ctgcaagctg
    20521 ctctctggtc aggctgtcag tcccctgacg tgcaagttta acaatgaaca gcagccatag
    20581 cacagccaga aagaggagaa agataccatc gcttcgacta agttgtccgt catagagtac
    20641 ggaaccggcc accacgctga ccaacaacat taagggtaat tcacggcgca gaacatcgga
    20701 atggacggta aaaggacgaa ccagcgcagc cagaccgagg atcagcaata tattgataat
    20761 gtttgagccg agggctgtac caacggctaa atcgcgttgt tcgtgcagag acgcggcaag
    20821 cgagacgatg atttctggta acgatgtacc aatactgacc accgtcatgc cgatgatcag
    20881 cggcgggatg ccaaaggttc ggcaaagaat agacgcggca aaaaccaggc ggtcggcact
    20941 gtaaacgacc aaaagtaaac caacaattaa cagtgccgta gctaaaagca tctaacgtcc
    21001 tttcttcagg tatactcgcc ggtccgctga agattttcag aaagccgtaa cggattctta
    21061 attttgactt tatgcggcta aaaagtaaaa caaatgccag ctttcgctaa ccacggcggg
    21121 taatattctg taaatatgtt gggttcaagg ttaaattgag cgccatgctt agaaaatcaa
    21181 cgcaagacga agggtgaatt atggagcagt ctgtggcgaa tttagtcgat atgcgcgatg
    21241 tcagttttac gcgtggcaat cgctgcatct tcgataatat ttccctgacc gtgccgcgag
    21301 ggaagatcac ggcgatcatg gggccatcgg gcatcggtaa aacgacgcta ctccgtctga
    21361 ttggcgggca aatcgcacca gatcatggtg agatcctttt cgatggtgag aatattccgg
    21421 cgatgtctcg ttcgcgcctg tatacagtgc gtaaacggat gagcatgtta tttcagtccg
    21481 gggcgttgtt cactgatatg aacgtatttg acaacgtcgc ctatccactg cgcgaacata
    21541 cccaacttcc cgcgccattg ttgcatagta cggtgatgat gaagctggag gccgtggggc
    21601 tgcgtggagc ggctaaacta atgccttctg aactttccgg tgggatggcg cggcgtgcag
    21661 cgctggcgcg tgcgattgcg ctggagccgg atctcatcat gtttgatgaa ccttttgttg
    21721 ggcaagatcc catcaccatg ggcgtactgg tgaagctgat ttctgagctg aacagcgcgc
    21781 tgggcgtgac ttgtgtggtg gtttctcacg atgtgccgga agtgttaagt attgcggatc
    21841 acgcctggat cctggcggac aaaaaaattg tcgctcatgg cagtgcccag gcgttgcaga
    21901 cgaatcctga tccgcgcgta cgtcagtttc tggacgggat agctgacggg cctgttccgt
    21961 tccgctatcc tgccggcgat tatcacgctg atcttttacc agggagttaa gccactcatg
    22021 ctgttaaatg cgctggcgtc gctcggacat aaagggatta aaaccctgag aacgttcggg
    22081 cgggccgggt taatgttatt caatgcgctg gtcggcaaac cggaatttcg caaacatgcg
    22141 ccgctgctgg tgcgccagct ctataatgtc ggcgtcctgt cgatgctgat tattgtagtt
    22201 tctggcgtgt tcatcggaat ggtgttgggg ctgcaaggtt atctggttct gaccacttat
    22261 agcgcggaaa ccagtctggg tatgctggtg gcgttatcgc tactgcgtga actggggccg
    22321 gtggttgccg cgctgttgtt tgccgggcgt gctggttcgg cgttaaccgc agaaatcggc
    22381 ctgatgcgcg ctacagagca actctccagt atggagatga tggcggtgga tccgctgcgt
    22441 cgggttattt ctccccgttt ctgggctggg gttatttcat taccactgtt gacggttatc
    22501 ttcgtcgccg tggggatctg gggcggatcg ctggtcggcg tcagttggaa aggcattgat
    22561 agcgggttct tctggtcggc aatgcaaaat gccgtcgact ggcgtatgga tctggtcaac
    22621 tgtctgatta agagcgtggt gttcgccatc acggtgacgt ggatttcgtt gtttaacggc
    22681 tacgacgcca tcccgacgtc tgccgggatt agccgggcaa ccactcgcac cgttgtccac
    22741 tcgtctctgg ctgttctggg gctggatttt gtgctgaccg cattgatgtt tgggaattga
    22801 gttcatgcaa acgaaaaaaa atgaaatttg ggtgggtatc tttttattag cagcactgct
    22861 ggcggcgctg tttgtttgcc tgaaggcggc gaacgtgacg tccatacgta ctgaatcgac
    22921 ctacacgctt tatgcgacgt tcgataacat tggcggcctg aaagcgcgct ctccggtcag
    22981 cattggtggt gttgttgtgg gccgggtggc ggatattacg ctggacccga aaacctatct
    23041 gccgcgcgta acgctggaaa ttgaacaacg ttataaccac attcctgata ccagttcgct
    23101 gagcattcgt acttccggcc tgctgggaga acaatatctg gcattaaacg tcggttttga
    23161 agacccggaa ctggggactg ctatcctgaa ggatggcgat acaattcagg acaccaagtc
    23221 tgcgatggtg ctggaagatc tcattggtca gttcctttac ggtagtaaag gcgatgacaa
    23281 taagaatagt ggcgatgcgc cagctgctgc gccaggtaat aatgaaacca ctgaacctgt
    23341 gggtacaacg aaataatttc aggagaaccg acgcatgttt aaacgtttaa tgatggtcgc
    23401 tttgctggtg attgcacctc tgagtgcggc aaccgcggca gaccagacca atccttataa
    23461 gctgatggac gaggcggcgc agaaaacgtt cgatcgcctg aagaatgagc aaccgcaaat
    23521 tcgggccaac ccggattatc tgcgtaccat tgttgatcag gaactgctgc catacgtaca
    23581 ggtgaaatac gccggtgcgc tggtgctggg ccagtattac aagagtgcga cccctgctca
    23641 acgtgaagcc tactttgccg ctttccgtga gtacctgaag caggcttacg gtcaggcgct
    23701 ggcgatgtat cacggtcaaa cctatcagat tgcgccagaa cagccgctgg gcgataaaac
    23761 cattgtgcct attcgcgtta ccattattga cccgaatggc cgtccgccgg tgcgtctgga
    23821 cttccagtgg cgtaaaaact cccagacggg aaactggcaa gcttacgaca tgattgctga
    23881 aggcgtcagt atgatcacca ccaaacaaaa cgagtgggga acgctgctgc gtaccaaagg
    23941 tatcgacggc ctgactgcgc aactgaaatc gatttctcaa cagaaaatca ctctggaaga
    24001 gaaaaaataa tgagcgagtc actgagctgg atgcagacgg gtgacacgct ggcgttatcc
    24061 ggagagctgg atcaggacgt tctgctaccg ctttgggaaa tgcgtgagga ggcggtgaag
    24121 gggattacct gcatcgatct tagccgtgtg tcccgcgtgg atacgggggg actggcactg
    24181 ctgctccatc ttatcgatct ggcgaaaaag cagggcaaca acgtgacgct tcagggggta
    24241 aacgacaaag tgtataccct cgcgaaattg tataatttgc ctgctgatgt tctgcctcgt
    24301 taaatttttt caggccggat atggcgttaa ccgcctgtcc ggcaaacctg cctgcaagtc
    24361 gagccttgtc tgatttatca gcgaggcttt ttgcttattt atgccagcgc aactttgctc
    24421 taagatgttt cgctggttta ttaactgatg attgaagatc ccatggaaaa taatgaaatt
    24481 cagagcgtgt tgatgaacgc tctctccctc caggaagtcc acgtttccgg cgatggcagc
    24541 cactttcagg ttattgccgt gggtgagttg tttgacggta tgagtcgggt taaaaaacag
    24601 cagacggtct atggcccgct gatggaatat attgcggata accgcattca tgctgtgtcg
    24661 atcaaagcgt atacccctgc ggagtgggcg cgcgatcgca aactgaacgg cttttgagct
    24721 atgggcgatt cgccggtagc ggatatgaat tgttaactga gaacaaacta aatggataaa
    24781 tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat
    24841 gctgctctgc ctattctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac
    24901 gtcccgaaac tgaaagacgt cgatacatca atgaaactgc taagccagct gggtgcgaaa
    24961 gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct
    25021 tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc
    25081 tttggtcagg ggcaagtttc actacctggc ggttgtacga tcggtgcgcg tccggttgat
    25141 ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt
    25201 aaagcttccg tcgatggtcg tctgaaaggc gcacatatcg tgatggataa agtcagcgtt
    25261 ggcgcaacgg tgaccatcat gtgtgctgca accctggcgg aaggcaccac gattattgaa
    25321 aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg
    25381 aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc
    25441 ggtgtctatc gcgtgctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg
    25501 atctctcgtg gcaaaattat ctgccgtaac gcgcagccag atactctgga cgccgtgctg
    25561 gcgaaactgc gtgatgctgg agcggacatc gaagtcggcg aagactggat tagcctggat
    25621 atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattcccg
    25681 accgatatgc aggcccagtt cacgctgttg aacctggtgg ctgaagggac cgggttcatc
    25741 accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg
    25801 cacgccgaaa tcgaaagcaa taccgttatt tgtcatggtg ttgaaaaact ttctggtgca
    25861 caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg
    25921 gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa
    25981 gacaaactgc gcgctttagg tgcaaatatt gagcgtgtga aaggcgagta atcgtctgag
    26041 agctggcagc cccgttcgcg gggctaccgc cagaccactc attttttcgg tttagtgtag
    26101 ctacgcatca actgcgttct gtcgataaac tcatgggtct gcggatcatg gtagcgtgat
    26161 ggccagataa cccaggggtc agttcccagg gctttcgcaa taatcatctc tcctttcggc
    26221 catgggcgcg ataatgcgtt tgccagcgtt gaggaactca aaccatttct gcgagattcc
    26281 gccgccattg acgttccttt cttgcgcaaa cctgcaatga tgtcagcggg atgccagtca
    26341 atgaaattac tttccatctt tcctcactcc ttaagttagt cgctttcatt cagaacactt
    26401 cttctgtcgg gcatcactat actcctgatg gtctattgcg ctaaaaagtt cctgtaaaga
    26461 gtgtgaatat tcagaatata taaagattta tcgccctttt ttcaggtgtt atagcgctga
    26521 attagtgttt ttatggaatt tactggaatc atcccgcgca ctgcgcggga tgaagggagg
    26581 ggattaacga tcgcgttgaa cagcgatgtg cgcgaggccg atgagtgctt ctcgccaagg
    26641 ggtgtccggg agcacctgta acgctgcgat ggctttgtct gcttcttcct cggcgcgctg
    26701 acgcgtccat tcaagagatc cacaagcgtt cattgcttcc agaaccggtt ccagaagatg
    26761 gcgaccgtta ccttgttcga tggcggtacg gatcatctgt gcctgttctg gtgtgccatg
    26821 atgcatcgca tgcagcagcg gcagcgtcgg tttaccttcg ttcagatcgt cgccgacgtt
    26881 tttacctaac tgttcgccat cggcattgta atcgagtaaa tcgtcgatca actggaaagc
    26941 agtgccgaga tagcgcccat aatcctgcag gcctttctcc tcctccggcg tacagccagc
    27001 cagaatcccg gaacactgcg cggcagcctc aaacagacgc gcggttttgc tatagataac
    27061 gcgcatgtag ttttcttcag tgatgtccgg atcgttaacg ttcatcagtt gcagaacttc
    27121 accttctgcg atgacgttta cagcttctga catgacttcc agcactttga gtgaaccgag
    27181 gctggtcatc atctggaacg cgcgggtata aataaaatcg cctaccagca cgctggcggc
    27241 attgccaaat gcggcgttgg cggtagcttt acccctgcgc atatctgatt catccacaac
    27301 gtcgtcgtgt agcagagtcg ccgtgtggat aaactcgatc agggcagcaa tggtgacatg
    27361 cgcatttccc tcatagccaa cagctcgtgc agccagtaca gcaatcatcg gacgaatacg
    27421 tttaccgccg ccgctgacga tgtaatagcc taactgattg atcagttgga cgtcggaatt
    27481 aagctgctca aggattgccg cattaacacc cgccatatct tgcgcggtta actcattgat
    27541 tttttctaaa ttcatcgcaa aagccgggct tttcgccccg attaccttac tgtgaacaaa
    27601 ggcaacttag ggtttatggt taggaatata gggtaattgt actgaaaaaa tggcacagat
    27661 aaacgttacc gtacaagttg tgtttttttc ttcgtgtatt gactgtagca cttgtcaaag
    27721 gcgtgcgttt tgcgtaatat tcgcgcccta ttgtgaatat ttatagcgca ctctgaatca
    27781 ttgaaaaggt gtgcgcggaa gcggagtttt atatgtacgc ggttttccaa agtggtggta
    27841 aacaacaccg agtaagcgaa ggtcagaccg ttcgcctgga aaagctggac atcgcaactg
    27901 gcgaaactgt tgagttcgct gaagtgctga tgatcgcaaa cggtgaagaa gtcaaaatcg
    27961 gcgttccttt cgttgatggc ggcgtaatca aagctgaagt tgttgctcac ggtcgtggcg
    28021 agaaagttaa aatcgttaag tttcgtcgtc gtaaacacta tcgtaagcag cagggccatc
    28081 gtcagtggtt cactgatgtg aaaattactg gcatcagcgc ctaagacctg aggagagatt
    28141 tcaaatggca cataaaaagg ctggcggctc cacacgtaac ggtcgcgatt cagaagctaa
    28201 acgcttgggc gttaagcgtt tcggtggcga atccgttctg gcgggtagca tcatcgttcg
    28261 tcaacgtggt accaaattcc acgctggcgc taacgtaggt tgcggtcgtg accacactct
    28321 gtttgctaaa gcagacggta aagtgaaatt cgaagttaaa ggcccgaaaa accgtaaatt
    28381 tatcagcatc gaagctgaat aagtttttcg cgtcccggta acggatgaaa gccccgcaac
    28441 acgttgcggg gctttttaca ttcgataacc ggtaaaaatg cgcatggtgc gacgttaatc
    28501 cgcaaatgcg ctttgcaggg aatacggtat gaagcagcag gcaggcattg gcattctttt
    28561 ggcgcttacc acagcaattt gctggggggc gttgccaatc gcaatgaagc aggtgctgga
    28621 ggtgatggaa cctccgacaa tcgtgtttta ccgtttcttg atggcgagta ttggccttgg
    28681 tgccattctt gcggtgaaga agaggttgcc gccattacgc gtgtttcgta agccacgctg
    28741 gttgattttg ttggcagtgg cgaccgccgg gctgtttggg aacttcatcc tgttcagctc
    28801 atccttgcaa tacctgagtc cgaccgcttc gcaggtgatt gggcaactct cgccagttgg
    28861 catgatggtt gccagcgtat ttatcctgaa agagaaaatg cgcagcactc aggttgtagg
    28921 ggcattgatg ctcctgagcg gtctggtgat gttttttaac accagtctgg tcgagatatt
    28981 tacaaagctc accgattaca cctggggagt tatctttggg gtcggtgcgg cgacggtttg
    29041 ggtgagttat ggcgtggcgc aaaaggtttt attgcgtcgg ctggcctcac cgcagatcct
    29101 gtttttactg tacactttat gtacaatcgc gctcttccct ctggcaaagc ctggagtgat
    29161 agcgcagctt agccactggc agctcgcatg tttaattttt tgcggactga ataccttggt
    29221 aggatatggc gccttggcgg aagcgatggc tcgctggcag gcagcgcagg tgagcgcgat
    29281 catcacgctc accccactgt ttacgctgtt tttttcagat cttttatcac tggcctggcc
    29341 cgatttcttc gccagaccga tgttaaacct tttaggttat ctcggtgcgt ttgtcgtggt
    29401 tgcgggcgcg atgtattccg ccattggtca tcgtatttgg ggcggattac gtaagcatac
    29461 aacggtggta tcgcaacccc gcgcaggcga atgatttacg gagaataaaa tgaagtttgt
    29521 tgatgaagca tcgattctgg tcgttgcagg tgatggcggt aatggttgcg tgagcttccg
    29581 ccgcgaaaag tatattccga aaggcggccc ggatggcggc gacggcggtg atggtggtga
    29641 cgtatggatg gaagccgacg agaacctgaa cacgcttatc gattatcgtt ttgaaaaatc
    29701 tttccgtgca gagcgcggtc agaacggcgc aagccgcgat tgtaccggta agcgcggtaa
    29761 agacgtgacg attaaagtgc cggtaggtac gcgtgtaatc gaccagggta ctggtgaaac
    29821 catgggtgat atgactaaac acggtcagcg tctgctggtt gctaagggcg gctggcacgg
    29881 tctgggcaat acccgtttca aatcgtcagt taaccgtaca ccacggcaga aaactaacgg
    29941 tacgccgggc gataagcgtg agctgctgct ggagctgatg ctgctggctg acgtcggtat
    30001 gttggggatg ccaaacgcgg gtaaatcgac ctttattcgt gcggtatcgg cggctaaacc
    30061 gaaagtggcg gattatccgt ttaccactct ggtgccaagt ctgggtgtgg tacgaatgga
    30121 caacgaaaag agcttcgttg ttgccgatat tccaggactg attgaaggcg ctgcggaagg
    30181 cgcaggtctg ggcattcgct tcctgaagca cctggaacgt tgccgcgtcc tgttgcacct
    30241 catcgatatc gatccgattg acggcaccga tccggttgaa aacgcgcgta ttattatcag
    30301 cgagctggaa aaatacagcc aggatctggc ggcgaaaccg cgttggttag tcttcaacaa
    30361 gatcgatctg ctggataagg cagaagccga agagaaagcg aaagtgatcg ctgaagcgct
    30421 gggctgggaa gataaatatt atctgatctc tgcggcgagc ggactgggcg tgaaagatct
    30481 ctgctgggat gtgatgacct ttatcattga aaacccggtc gtgcaggctg aagaagcgaa
    30541 acagccagag aaagtcgaat tcatgtggga tgattatcac cgtcagcagc ttgaagagat
    30601 tgctgaagag gatgatgaag actgggatga cgactgggac gaagacgacg aagaaggcgt
    30661 tgagttcatt tacaagcgtt aatcactaat gaccaatgcc aaataagcct gatgcgctac
    30721 acttatcagg cctacaagat gattgcaata tattgaattt gcacgatttt gtaggccgga
    30781 taaggcgttc acgccgcatc cggcataaac aaagcgcact ttgtcaataa tctgaaaccc
    30841 cggccatgcg ccggggtttc tttttgacta attgttctga taaatatctt tgtacaaacg
    30901 gctttcaaaa cgcactaacg gaatacggcg attacgctga tccgcgggtt ttactgcata
    30961 gccagaaaga tattgtacaa atgccattcg ttgcccgctc gctgtggtaa tgaatcccgc
    31021 caggttatat accccctgca acgaaccggt tttcgctgag actttgccat ccacgcccgc
    31081 ctgatgcaga cctgcacggt actgcaaaga gccgtcatag cccgcaagcg gcagcatgga
    31141 gataaagtta agttcattgt cgtgttgggc aatgtattgc agcacctgca tcatggtggc
    31201 gggggcaatc aggttatgcc gcgaaagccc tgaaccatcg gcaataatgg tgttaccaat
    31261 atcgacaccg gcttgctggc gcaggatctg acgcacggcg tccgacccag cccgccatgt
    31321 tccaggcaca ttgaagcgcg catggccgat catgcggaaa accgtatcgg cgatcatgtt
    31381 gtccgacttt ttcagcataa tcttaagcag atcgtgcagc ggggccgact gtttactggc
    31441 aactaccgtt ccaggttcgt taacctgagt ctggcgcagc agtgttccgc tccaggtgat
    31501 acccgcctgt tttaactcat ctttcagaat tgcaccggca tagctggctc catcctgcac
    31561 ggcaaaagcc aacgggagcg gctcagaacg ttgtggcagg catcccgtca gcgtaaagcg
    31621 gttcaggtcg cttggcacca catccagttc gcagtattgc gcttcggcag aaccacgggg
    31681 gagggtgcgt acctggctga acatcgtaac ggggtaataa gatgccacgc gtataaaagc
    31741 catatcacca ggctttgggg cactgtagag cgagacggag aaacagttgc ggtcaactat
    31801 ggcggcagca ggcggagcgc taaagcattg tgtcatgtca ttccatggcc agccgggggc
    31861 tttatcgtgg ctggcgaaaa tggaggtatc tatcaacaca ttgccatcga tttggttgac
    31921 gccagatttt ttcaaagtcg cgaccatatt gcgaatatcc tgacgtttta acgtcggatc
    31981 ggcaccaaat cgcgccacta agtcaccctt aagtacgccg ttttccacat tgcctttggt
    32041 ttcaagcgtc gtggtgaaac gaaaatcggg gccgagttga atcaacgccg ccagcgcagt
    32101 aatcactttc tgggtactgg cgggcagcgc catctgctga ctgtggtaat caatagcggg
    32161 ggccgacgca ccgacttttt gcaccatcag ggcaaggttg gcacctgcgg ggagttgagt
    32221 aacgtactca tcaacattcg cggcctgaac actgaacgct atacagctgg tcaatccgat
    32281 gataaatctg gaaaatcgca taatctcgcg ctaacaacct ggaatcgagc cgtcatacta
    32341 cggcgcaacg ccctataaag taaacgatga cccttcggga acttcagggt aaaatgacca
    32401 tcaaaatgtg aattgtagct gacctgggac ttgtacccgg gtcggtattt ttttgcttct
    32461 ggtcccggtg aggagttatg ccgggcaggc cgaacagccg gggtgggtga agacttgccc
    32521 catcaggaat attcaagagg tataacaaat gcaagctatt ccgatgacct tacgcggcgc
    32581 tgaaaaatta cgcgaagagc tggattttct gaaatctgtg cgccgtcctg aaatcattgc
    32641 tgctatcgcg gaagcgcgtg agcatggcga cctgaaagaa aacgccgaat accacgcagc
    32701 tcgtgaacag cagggcttct gcgaaggccg tatcaaagac atcgaagcca agctgtcaaa
    32761 cgcgcaggtg attgatgtca ccaaaatgcc caacaatggg cgcgttattt ttggtgctac
    32821 cgtaacggtg ctgaatctgg attctgacga agaacagact tatcgcatcg ttggcgatga
    32881 cgaagctgac tttaaacaaa acctgatttc tgtaaactcg cctattgctc gtggcctgat
    32941 cggcaaagaa gaagatgatg ttgtggtcat caaaacgccg ggcggcgaag tagaatttga
    33001 agtaattaag gtggaatacc tgtaagaatt acccaatact caagatgttg atgtattgta
    33061 aagaaaggaa aaaggccgct ctgcggcctt ttatcaacga acagagcgtg gcattttgct
    33121 ctcctgcctg cggaaaaccc ctcgttttac acagcaaatg tgtgtaactt taggataatc
    33181 ttagcgtggc agcgagattt tacgttcttt agttgggcga taaagcacca gcgttttacc
    33241 gatgacctgt acattacagg cgccggtttc gcgcacgata gcttccacga tcaaggtttt
    33301 tgtttcgcga tcttcggtgg cgattttcac cttgatgagt tcatggtgct ctaacgcttg
    33361 ttcaatctcg gccagcaccc cttcggtcaa accattactg ccaagcagaa caactggctt
    33421 gagcggatgt gccagacctt tcaggtgctg tttttgttta gtactcagat tcatcgtatt
    33481 ttttgcttac gttgggattg aaaacgggtc attctaccgc catctcccat atatcaccaa
    33541 ataggcgcgt aaaaatttac gcaattggtt acgatgagtt atccccatgg gaaagttaaa
    33601 tgacaggtaa gaagcgttct gccagctcca gccgctggct tcaggaacac tttagcgata
    33661 aatatgttca acaggcacag aaaaagggat tacgttcccg tgcctggttt aaacttgatg
    33721 aaatacagca aagtgacaaa ctctttaaac cgggaatgac ggttgtcgac cttggtgctg
    33781 ctccgggtgg ttggtcacaa tatgtggtca cccaaattgg cggcaaaggc cgcatcatcg
    33841 cttgcgatct tttacctatg gatcctatcg ttggtgtgga ctttcttcag ggcgattttc
    33901 gtgatgaact ggtgatgaaa gcactgctgg agcgcgttgg cgacagcaaa gtccaggttg
    33961 tcatgtccga tatggcacca aacatgagcg gaacaccggc ggtggatatc ccccgagcca
    34021 tgtatctggt ggaactggcg ctagaaatgt gtcgtgatgt attagcgcca ggtggcagtt
    34081 ttgtagtgaa ggtgttccag ggcgaaggtt tcgatgagta tctaagggaa attcgctccc
    34141 tgtttacgaa ggtcaaagtt cgtaagccgg actcttctcg tgcacgttcg cgagaagtgt
    34201 atattgtagc gaccgggcgt aaaccctaac cgggagattt cagacgaaag tttgaaagat
    34261 gctggatata gagtatcctg acgctgtttt taacacagtt gtaataagag gttaatccct
    34321 tgagtgacat ggcgaaaaac ctaatactct ggctggtcat tgccgttgtg ctgatgtcag
    34381 tattccagag ctttgggccc agcgagtcta atggccgtaa ggtggattac tctaccttcc
    34441 tacaagaggt caataacgac caggttcgtg aagcgcgtat caacggacgt gaaatcaacg
    34501 ttaccaagaa agatagtaac cgttatacca cttacattcc ggttcaggat ccgaaattac
    34561 tggataacct gttgaccaag aacgtcaagg ttgtcggtga accgcctgaa gaaccaagcc
    34621 tgctggcttc tatcttcatc tcctggttcc cgatgctgtt gctgattggt gtctggatct
    34681 tcttcatgcg tcaaatgcag ggcggcggtg gcaaaggtgc catgtcgttt ggtaagagca
    34741 aagcgcgcat gctgacggaa gatcagatca aaacgacctt tgctgacgtt gcgggctgcg
    34801 acgaagcaaa agaagaagtt gctgaactgg tagagtatct gcgcgagccg agccgcttcc
    34861 agaaactcgg cggtaagatc ccgaaaggcg tcctgatggt cggtcctccg ggtaccggta
    34921 aaacgttgct ggcgaaagcg attgcaggcg aagcgaaagt tccgttcttt actatctccg
    34981 gttctgactt cgtagaaatg ttcgtcggtg tgggtgcatc ccgtgttcgt gacatgttcg
    35041 aacaggcgaa gaaagcggca ccgtgcatca tctttatcga tgaaatcgac gccgtaggcc
    35101 gccagcgtgg cgctggtctg ggcggtggtc acgatgaacg tgaacagact ctgaaccaga
    35161 tgctggttga gatggatggc ttcgaaggta acgaaggtat catcgttatc gccgcgacta
    35221 accgtccgga cgttctcgac ccggccctgc tgcgtcctgg ccgtttcgac cgccaggttg
    35281 tggtcggctt gccagatgtt cgcggtcgtg agcagatcct gaaagttcac atgcgtcgcg
    35341 taccattggc acccgatatc gacgcggcaa tcattgcccg tggtactcct ggtttctccg
    35401 gtgctgacct ggcgaacctg gtgaacgaag cggcactgtt cgctgctcgt ggcaacaaac
    35461 gcgttgtgtc gatggttgag ttcgagaaag cgaaagacaa aatcatgatg ggtgcggaac
    35521 gtcgctccat ggtgatgacg gaagcgcaga aagaatcaac ggcttaccac gaagcgggtc
    35581 atgcgattat cggtcgcctg gtgccggaac acgatccggt gcataaagtg acgattatcc
    35641 cacgcggtcg tgcgctgggt gtgactttct tcttgcctga gggcgacgca atcagcgcca
    35701 gccgtcagaa actggaaagc cagatttcta cgctgtacgg tggtcgtctg gcagaagaga
    35761 tcatctacgg gccggaacat gtttctaccg gtgcgtccaa cgatattaaa gttgcgacca
    35821 acctggcacg taacatggtg actcagtggg gcttctctga gaaattgggt ccactgctgt
    35881 acgcggaaga agaaggtgaa gtattcctcg gccgtagcgt agcgaaagcg aaacatatgt
    35941 ccgatgaaac tgcacgtatc atcgaccagg aagtgaaagc actgattgag cgtaactata
    36001 atcgtgcgcg tcagcttctg accgacaata tggatattct gcatgcgatg aaagatgctc
    36061 tcatgaaata tgagactatc gacgcaccgc agattgatga cctgatggca cgtcgcgatg
    36121 tacgtccgcc agcgggctgg gaagaaccag gcgcttctaa caattctggc gacaatggta
    36181 gtccaaaggc tcctcgtccg gttgatgaac cgcgtacgcc gaacccgggt aacaccatgt
    36241 cagagcagtt aggcgacaag taagttcccg catcagatga ctgtatttgt accgaaaacc
    36301 ccggggcgtg ctccggggtt ttttcttatc aattcatacc agggataaca taatgaaact
    36361 ctttgcccag ggtacttcac tggaccttag ccatcctcac gtaatgggga tcctcaacgt
    36421 cacgcctgat tccttttcgg atggtggcac gcataactcg ctgatagatg cggtgaaaca
    36481 tgcgaatctg atgatcaatg ctggcgcgac gatcattgac gttggtggcg agtccacgcg
    36541 cccaggggcg gcggaagtta gcgttgaaga agagttgcaa cgtgttattc ctgtggttga
    36601 ggcaattgct caacgcttcg aagtctggat ctcggtcgat acatccaaac cagaagtcat
    36661 ccgtgagtca gcgaaagttg gcgctcacat tattaatgat atccgctccc tttccgaacc
    36721 tggcgctctg gaggcggctg cagaaaccgg tttaccggtt tgtctgatgc atatgcaggg
    36781 aaatccaaaa accatgcagg aagctccgaa gtatgacgat gtctttgcag aagtgaatcg
    36841 ctactttatt gagcaaatag cacgttgcga gcaggcgggt atcgcaaaag agaaattgtt
    36901 gctcgacccc ggattcggtt tcggtaaaaa tctctcccat aactattcat tactggcgcg
    36961 cctggctgaa tttcaccatt tcaacctgcc gctgttggtg ggtatgtcac gaaaatcgat
    37021 gattgggcag ctgctgaacg tggggccgtc cgagcgcctg agcggtagtc tggcctgtgc
    37081 ggtcattgcc gcaatgcaag gcgcgcacat cattcgtgtt catgacgtca aagaaaccgt
    37141 agaagcgatg cgggtggtgg aagccactct gtctgcaaag gaaaacaaac gctatgagta
    37201 atcgtaaata tttcggtacc gatgggattc gtggtcgtgt aggggatgcg ccgatcacac
    37261 ctgattttgt gcttaagctg ggttgggccg cgggtaaagt gctggcgcgc cacggctccc
    37321 gtaagattat tattggtaaa gacacgcgta tttctggcta tatgctggag tcagcactgg
    37381 aagcgggtct ggcggcagcg ggcctttccg cactcttcac tggcccgatg ccaacaccgg
    37441 ccgtggctta tctgacgcgt accttccgcg cagaggccgg aattgtgata tctgcatcgc
    37501 ataacccgtt ctacgataat gggattaaat tcttctctat cgacggcact aaactgccgg
    37561 atgcggtaga agaggccatc gaagcggaaa tggaaaagga gatcagctgc gttgattcgg
    37621 cagaactggg taaagccagc cgtatcgttg atgccgcggg tcgctatatc gagttttgca
    37681 aagccacgtt cccgaacgaa cttagcctca gtgaactgaa gattgtggtg gattgtgcaa
    37741 acggtgcgac ttatcacatc gcgccgaacg tgctgcgcga actgggggcg aacgttatcg
    37801 ctatcggttg tgagccaaac ggtgtaaaca tcaatgccga agtgggggct accgacgttc
    37861 gcgcgctcca ggctcgtgtg ctggctgaaa aagcggatct cggtattgcc ttcgacggcg
    37921 acggcgatcg cgtgattatg gttgaccatg aaggcaataa agtcgatggc gatcagatca
    37981 tgtatatcat cgcgcgtgaa ggtcttcgtc agggccagct gcgtggtggt gctgtgggta
    38041 cattgatgag caacatgggg cttgaactgg cgctgaaaca gttaggaatt ccatttgcgc
    38101 gcgcgaaagt gggtgaccgc tacgtactgg aaaaaatgca ggagaaaggc tggcgtatcg
    38161 gtgcagagaa ttccggtcat gtgatcctgc tggataaaac cactaccggt gacggcatcg
    38221 ttgctggctt gcaggtgctg gcggcgatgg cacgtaacca tatgagcctg cacgaccttt
    38281 gcagcggcat gaaaatgttc ccgcagattc tggttaacgt acgttacacc gcaggtagcg
    38341 gcgatccgct tgagcatgag tcagttaaag ccgtgaccgc agaggttgaa gctgcgctgg
    38401 gcaaccgtgg acgcgtgttg ctgcgtaaat ccggcaccga accgttaatt cgcgtgatgg
    38461 tggaaggcga agacgaagcg caggtgactg aatttgcaca ccgcatcgcc gatgcagtaa
    38521 aagccgttta aagcgttaga taactggcta aaaaggcggc gattgtcgcc ttttttctcg
    38581 gcgaacacat cggttttgct gtttttttcc gcagttgata caatgcgata aaattgccct
    38641 tgcgaaggtc attcgttttg gttagtattc acacccgctt cagtgggaaa gattaaaaac
    38701 tcccgcttta ttggttgaag cattggtacg cggcaactcc gcaaggaaca ggttgattat
    38761 gtacgaagct cttttagtag ttttccttat tgtggcaatt ggccttgttg gtctgatcat
    38821 gctgcagcaa ggtaaaggcg ctgatatggg agcctccttc ggagcaggcg cttccgctac
    38881 gctgtttggt tcaagtggtt ctggtaactt catgacccgc atgacggcgc tgctggcaac
    38941 gttattcttc atcatcagtc tggtgctggg taacatcaat agcaacaaaa ccaatagagg
    39001 tagcgaatgg gaaaatctga gtgcaccggc gaaaaccgaa caaactcagc cagctgctcc
    39061 ggctaagccg accagcgata tcccgaacta aaaagtagta tccgtgccga ggtggtggaa
    39121 ttggtagaca cgctaccttg aggtggtagt gcccaatagg gcttacgggt tcaagtcccg
    39181 tcctcggtac caaattccag aaaagagacg ctgaaaagcg tctcttttcg ttttggtcct
    39241 ggtctgatga atggttgtca ggtatctgta tctcgtccag ttgctttact cgtagctggt
    39301 accttgaaac gatggtgccg gtacgcctta gttataaact tttccctact gctgacataa
    39361 ttggcacttt attgtgtcaa ctccagttcc ggtacgaata cggatatgtt tacttctcga
    39421 taaaccatcc tgctcctgtc agtataacta ctgatggcta cgttctgcgt aatgtttaga
    39481 gacgcattga taatgttatt taattactga gaacgcaatg gtcgcatcat ttaaaaagaa
    39541 aaaactaaca gtgtttcggt ctgatatgga tcagagttga aaaacatgtc tgatataaaa
    39601 ttgaacattg ttgataattg tttatattgg ttgtgaactt ggatttctgg atgaagtatt
    39661 caacaaattt gctagaagat tttaaatctc attggttgtt gtatctttgt gttattgttt
    39721 ttggtattac gaacttagtc gcctcttccg gagcgcatat ggttcagcga ttgctgttct
    39781 tcgtcctgac catcctggtt gttaaacgta tatcatccct tccgcttcgc ctgcttgttg
    39841 ccgcaccatt tgtgttactg actgcggcag acatgagtat tagcctctat tcatggtgta
    39901 cctttggtac aactttcaat gatggatttg cgattagtgt gctgcagagt gatccggatg
    39961 aagttgtgaa aatgctgggg atgtatatcc cttatctatg tgcttttgct tttttatccc
    40021 ttcttttttt ggcagtaata ataaaatatg atgtttcctt gccgacaaaa aaagtgacag
    40081 gaatattatt gctgattgtc atttcgggca gtttattttc cgcttgtcaa tttgcttata
    40141 aagatgcaaa aaataaaaaa gcgttcagtc catatatact agcgtcgtga tttgctacct
    40201 atacgccgtt tttcaatctc aactattttg ctttagcagc gaaagagcat
//
LOCUS       DAEBXD010000029        39747 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEBXD010000029 DAEBXD010000000
VERSION     DAEBXD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39747
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.029"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..157
                     /locus_tag="KJL94_001561"
     CDS             <1..157
                     /locus_tag="KJL94_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4409962.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            386..976
                     /gene="uidR"
                     /locus_tag="KJL94_001562"
     CDS             386..976
                     /gene="uidR"
                     /locus_tag="KJL94_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBD4409963.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1364..3037
                     /gene="uidA"
                     /locus_tag="KJL94_001563"
     CDS             1364..3037
                     /gene="uidA"
                     /locus_tag="KJL94_001563"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBD4409964.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3073..4098
                     /gene="uidB"
                     /locus_tag="KJL94_001564"
                     /pseudo
     CDS             3073..4098
                     /gene="uidB"
                     /locus_tag="KJL94_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4137..5402
                     /gene="uidC"
                     /locus_tag="KJL94_001565"
     CDS             4137..5402
                     /gene="uidC"
                     /locus_tag="KJL94_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBD4409965.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5628..7136)
                     /locus_tag="KJL94_001566"
     CDS             complement(5628..7136)
                     /locus_tag="KJL94_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD4409966.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KJL94_001567"
     CDS             complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KJL94_001567"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBD4409967.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8611..10257
                     /gene="fumB"
                     /locus_tag="KJL94_001568"
     CDS             8611..10257
                     /gene="fumB"
                     /locus_tag="KJL94_001568"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD4409968.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10400..11803
                     /gene="fumC"
                     /locus_tag="KJL94_001569"
     CDS             10400..11803
                     /gene="fumC"
                     /locus_tag="KJL94_001569"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBD4409969.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KJL94_001570"
     CDS             complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KJL94_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBD4409970.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPTTLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KJL94_001571"
     CDS             complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KJL94_001571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBD4409971.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14285..15604)
                     /locus_tag="KJL94_001572"
     CDS             complement(14285..15604)
                     /locus_tag="KJL94_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBD4409972.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15829..16572)
                     /gene="rstA"
                     /locus_tag="KJL94_001573"
     CDS             complement(15829..16572)
                     /gene="rstA"
                     /locus_tag="KJL94_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBD4409973.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16701..17036
                     /locus_tag="KJL94_001574"
     CDS             16701..17036
                     /locus_tag="KJL94_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBD4409974.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17033..17755)
                     /gene="folM"
                     /locus_tag="KJL94_001575"
     CDS             complement(17033..17755)
                     /gene="folM"
                     /locus_tag="KJL94_001575"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBD4409975.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17792..19174)
                     /locus_tag="KJL94_001576"
     CDS             complement(17792..19174)
                     /locus_tag="KJL94_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4409976.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19360..20304)
                     /locus_tag="KJL94_001577"
     CDS             complement(19360..20304)
                     /locus_tag="KJL94_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4409977.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20828..22360
                     /gene="pntA"
                     /locus_tag="KJL94_001578"
     CDS             20828..22360
                     /gene="pntA"
                     /locus_tag="KJL94_001578"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBD4409978.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22371..23759
                     /gene="pntB"
                     /locus_tag="KJL94_001579"
     CDS             22371..23759
                     /gene="pntB"
                     /locus_tag="KJL94_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBD4409979.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23784..24818)
                     /gene="tqsA"
                     /locus_tag="KJL94_001580"
     CDS             complement(23784..24818)
                     /gene="tqsA"
                     /locus_tag="KJL94_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBD4409980.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25229..25594
                     /gene="mdtJ"
                     /locus_tag="KJL94_001581"
     CDS             25229..25594
                     /gene="mdtJ"
                     /locus_tag="KJL94_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBD4409981.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25581..25910
                     /gene="mdtI"
                     /locus_tag="KJL94_001582"
     CDS             25581..25910
                     /gene="mdtI"
                     /locus_tag="KJL94_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBD4409982.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25949..26770)
                     /locus_tag="KJL94_001583"
     CDS             complement(25949..26770)
                     /locus_tag="KJL94_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBD4409983.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26870..26953)
                     /locus_tag="KJL94_001584"
     CDS             complement(26870..26953)
                     /locus_tag="KJL94_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409984.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27046..27381)
                     /locus_tag="KJL94_001585"
     CDS             complement(27046..27381)
                     /locus_tag="KJL94_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBD4409985.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27949..28206)
                     /locus_tag="KJL94_001586"
     CDS             complement(27949..28206)
                     /locus_tag="KJL94_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4409986.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28222..29031)
                     /locus_tag="KJL94_001587"
     CDS             complement(28222..29031)
                     /locus_tag="KJL94_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4409987.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29138..30040
                     /locus_tag="KJL94_001588"
     CDS             29138..30040
                     /locus_tag="KJL94_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4409988.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30175..31395
                     /gene="mlc"
                     /locus_tag="KJL94_001589"
                     /gene_synonym="dgsA"
     CDS             30175..31395
                     /gene="mlc"
                     /locus_tag="KJL94_001589"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBD4409989.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31520..32215
                     /gene="bioD"
                     /locus_tag="KJL94_001590"
     CDS             31520..32215
                     /gene="bioD"
                     /locus_tag="KJL94_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD4409990.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32168..33460)
                     /gene="clcB"
                     /locus_tag="KJL94_001591"
     CDS             complement(32168..33460)
                     /gene="clcB"
                     /locus_tag="KJL94_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBD4409991.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33619..34233)
                     /gene="dmsD"
                     /locus_tag="KJL94_001592"
     CDS             complement(33619..34233)
                     /gene="dmsD"
                     /locus_tag="KJL94_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBD4409992.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34276..35130)
                     /locus_tag="KJL94_001593"
     CDS             complement(34276..35130)
                     /locus_tag="KJL94_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD4409993.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35132..35749)
                     /gene="dmsB"
                     /locus_tag="KJL94_001594"
     CDS             complement(35132..35749)
                     /gene="dmsB"
                     /locus_tag="KJL94_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD4409994.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35760..37798)
                     /locus_tag="KJL94_001595"
                     /pseudo
     CDS             complement(35760..37798)
                     /locus_tag="KJL94_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37859..38968)
                     /locus_tag="KJL94_001596"
     CDS             complement(37859..38968)
                     /locus_tag="KJL94_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD4409995.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38951..>39747
                     /locus_tag="KJL94_001597"
     CDS             38951..>39747
                     /locus_tag="KJL94_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4409996.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSASL"
BASE COUNT        10120 a         9634 c        10173 g         9820 t
ORIGIN      
        1 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
       61 tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
      121 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacgg
      181 tcgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
      241 ggcagccaaa tcacggctat tggttaacca attgctgggt gaaaagtata cgaatagagt
      301 gtaccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg gtaaactagt
      361 tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag cacaaccgac
      421 acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat ttcacagtgc
      481 ctcgatgaaa gccatctgta aatcttgcgc cattagtcct gggacgctct atcaccattt
      541 catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga gggcgctggc
      601 tcgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg agtctattgt
      661 ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta tggcggaagg
      721 gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga tcactgaatt
      781 tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag acatcaacac
      841 ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg atatcgaagc
      901 ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga ttggcggtat
      961 attaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc cgcgtttcat
     1021 atcgtaattt ctctgtgcaa aaattatcct ccccgtgttc ggagaattcc cccccaaaat
     1081 attcaccgta gccatatgtc atgagagttt atcgttccca atactctcga acgaacgttc
     1141 gattgcttat tttatggctt ctgtcaacgc tgttttaaag attaatgcga tccatatcac
     1201 gctgtgggta ttgcagtttt tggttttttg atcgcggtgt cagttctttt tatttccatt
     1261 tctcttccat gggtttctca cagataactg tgtgcaacac agaattggtt aactaatcag
     1321 attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac gtcctgtaga
     1381 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     1441 aaactgtgga attgatcagc gttggtgcga aagcgcgtta caagaaagcc gggcaattgc
     1501 agtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgtgggcaa
     1561 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     1621 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     1681 gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa
     1741 aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg
     1801 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     1861 ctatgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     1921 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     1981 ggtggccaat ggtgatatca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     2041 tggacaaggc accagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     2101 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     2161 gctgcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     2221 accgttctac tttactggct ttggtcgtca tgaagatgcg gatttgcgag gcaaaggatt
     2281 cgataacgtg ctgatggtgc acgatcacgc attaatggac tggattgggg ccaactccta
     2341 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     2401 cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc tctttaggca ttggtttcga
     2461 agcgggcaac aagccgaaag aactgtacag cgaagaggct gtcaacgggg aaactcagca
     2521 ggcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     2581 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     2641 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     2701 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     2761 ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     2821 aaaagaactg ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     2881 cgtggatacg ttagccggcc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     2941 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     3001 acaggtatgg aatttcgccg attttgcgac ctcgtaaggc atattgcgcg ttggcggtaa
     3061 caattgacct acatgggcct cgggctttgc tacagcctgg tgaatattcc ttatggttca
     3121 cttgctaccg cgatgaccca acaaccacaa tctcgcgccc gtctgggcgc ggctcgtggg
     3181 attgccgctt cattgacctt tgtatgcctg gcatttctga taggaccgag cattaagaac
     3241 tccagcccgg aagagatggt gtcggtatac catttctgga cgattgtgct ggtgattgcc
     3301 ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg aaaatgtggt acgtatcgtg
     3361 gcgcagccgt cattgaagat cagtttgcaa accctgaaac ggaatcgccc gctgtttatg
     3421 ttgtgcatcg gtgcgctgtg tgtgctgatt tcgaccttcg cggtcagcgc ctcgtcgttg
     3481 ttctacgtgc gctatgtgtt aaatgatacc gggctgttca ctgtgctggt actggtgcaa
     3541 aacctggttg gtactgtggc atcggcaccg cttgtgccgg ggatggtcgc gaggatcggt
     3601 aaaaagaata ccttcctgat tggcgctttg ctgggaacct gcggttatct gctattcttc
     3661 tgggtttccg tctggtcact gccggtggcg ttggttgcgt tggccatcgc ttcaattggt
     3721 cagggcgtta ctatgactgt gatgtgggca ctggaagctg ataccgtaga atacggtgaa
     3781 tacctgaccg gcgtgcgaat tgaagggctc acctattcac tattctcatt tacccgtaaa
     3841 tgcggtcagg caatcggagg ttcaattcct gcctttattt tgggattaag cggatatatc
     3901 gccaatcagg tgtaaacgcc ggaagttatt atgggcatcc gcacatcaat tgccttagta
     3961 ccttgcggat ttatgctact ggcattcgtt attatctggt tttatccgct cacggataaa
     4021 aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa aagtgcagca gcaattaatc
     4081 agcgatatca ctaattaata ttcaataaaa ataatcagaa catcaaaggt gcaactatga
     4141 gaaaaatagt ggccatggcc gttatttgcc tgacggctgc ctctggcctt acctctgctt
     4201 acgcggcgca actggctgac gatgaagcgg gactacgcat cagactgaaa aacgaattgc
     4261 gcagggctga taagcccagt gctggcgcgg gaagagatat ttacgcatgg gtacagggag
     4321 gattgctcga tttcaatagt ggttattatt ccaatattgt tggcgttgaa ggcggagcgt
     4381 attatgttta taaattaggt gctcgtgctg atatgagtac ccggtggtat cttgatggtg
     4441 ataaaagttt tggctttgcc ctgggggcag taaaaataaa acccagtgaa aatagcctgc
     4501 ttaaattagg tcgcttcggg acggattata gttatggtag cttaccttat cgtattccgt
     4561 taatggttgg cagttcgcaa cgtacattac cgacagtttc tgaaggagca ttaggttatt
     4621 gggctttaac gccaaatatt gatctgtggg gaatgtggcg ttcacgagta tttttatgga
     4681 ctgattcaac aaccggtatt cgtgatgaag gggtgtataa cagccagacg ggaaaatacg
     4741 ataaacatcg cgcacgttct tttttagccg ccagttggca tgatgatacc agtcgctatt
     4801 ctctgggggc atcggtacag aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa
     4861 gcataccgct cgacccgaat tatacgttga aaggggagtt gctcggcttt tacgcgcagc
     4921 tcgaaggttt aagtcgtaat accagccagc ccaatgaaac ggcgttggtt agtggacaat
     4981 tgacctggaa tgcgccgtgg ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa
     5041 tgaatggtgc cgtggtggat accgacattg gctatccctt ttcattaagt cttgatcgta
     5101 accgtgaagg aatgcagtcc tggcaattag gtgccaacta ccgtgtaact ccgcaattta
     5161 cgctgacatt tgcaccgatt gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga
     5221 ttgaaggcgc gggtatctta ggtggtatga actatcgggt cagcgaaggg ccgttacaag
     5281 ggatgaattt ctttcttgct gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg
     5341 gcgatcgcct gaattactgg gatgtgaaaa tgagtattca gtatgacttt atgctgaagt
     5401 aagaaataac gccggagaga aaaacctccg gcgtttcaga ttgttgacaa agtgcgcgtt
     5461 ttttatgccg gatgcggcgt aaacgcctta tccagcctac aaaaactcat aaattcaaag
     5521 tgttgcagga aaaggtaggc ctgataagcg tagcgcatca ggcaatctct ggtttgtttt
     5581 cagatgaaaa cgccggagtg aaaattctcc ggcgttttgg ccgtgaatta ctgctgcgga
     5641 attgccggta cagccggaac gttaagagct ggcatcgcaa acatgccaac aaaatcttct
     5701 aacgacattt tctgcccatt taacgttatc tgaccgttag catattgcag gctggtggtg
     5761 atggtattgt cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca
     5821 acttgctgtt tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc
     5881 tgagtcatca actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta
     5941 accgaacgat ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct
     6001 ttcaggaaca gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc
     6061 gcgatagtaa tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc
     6121 gtcactttct cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc
     6181 tgagtttgcg cgttatattg ctggctaaac tgatgccagg cttcaccatc aatctggccg
     6241 acttttaaag tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc
     6301 gagtaatcca gttggctatt gatcgtttta ccgtcattga ccagatccga tttaccgctg
     6361 atctccatgc cttccagcag tgccagttct ttgccttcca ctgaaatggt cattttttcc
     6421 agtgacagtt tttggtttcc tacgcgctca ccaaaacttc ccagcgtgct ggaaccgtcg
     6481 gttttcagat tattaaaggt caactgcact ttctggttgt attcgttaac tgcgtctatc
     6541 cgaccacttt gcgcctctcc ggaaagggag atggctttgc cgtctctgtc agcatttaac
     6601 tggaactcgc cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggattg
     6661 agcgaaatat cggaactgga atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc
     6721 gtttcacctt ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt
     6781 tgaattgatg ccatcgacgg gatcaggttc agttttttaa gctgggcaag cgggaaggga
     6841 ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggattttct
     6901 ttcccggcaa tgggtttcac caacagttgc aactggctgc tgaatacgcc gcgatgatag
     6961 ttttgataac tcacttccag gttggattca ggagctgtca gtttgagttg cgcgttcgcc
     7021 tgcgcgacca tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct
     7081 gtccagacta cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg
     7141 attatccata aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata
     7201 agcttagcaa gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc
     7261 taaacggccg tggcctttga cagtcaccgg tgattcgttg gcggcaataa acgctgattc
     7321 acccggttta agctgtaact gctgagaacc tttccacaac gttgcatcgc cttcgacgca
     7381 gaacaaaatg gcggcactct gctggctgat ggtggtttct ttatcactaa ggtcatgcag
     7441 cgagaaggca aaatcatcca caggaatcgg gaagtccagt tctgcacctt gtttcaccgg
     7501 ctgggtcaac aactggttag ccggtttggc ttcgaatttc acattggcaa ccagttccgg
     7561 aatatcaatg tatttaggcg tcagacctgc acgcagcacg ttatcggagt tcgccatcac
     7621 ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc
     7681 gccaggattc aatttcacca cattcagtaa cagcggggag aacaggccgc tgtcttccgg
     7741 gtaaaattca gaaattaaac gaatcgtttg ccacggttca ccctgctgac tgtcgagcgc
     7801 cgattttaaa atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggttggc
     7861 gaacagttcg cttaaacgtt cggcatgtgg ctgttgtaaa aagtgagcaa tcgccggatg
     7921 tgcaccggcg accggctgga gtagggagac aatctcggaa aattcacgaa acgcgttcat
     7981 cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt
     8041 acgctcggcg gcatccatcg ggatacctgc ggcattttct ttggcaaaac cgatttcaga
     8101 attgtgtttg tttggatgaa cctgaatgga gagtggctgt gctgcgcata atactttgaa
     8161 caggaaaggc agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatc
     8221 actctcaatc acatcacgca gtgaaacgat atctccggcg gcattctgca ctcgtgaact
     8281 gcttttcgga tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat
     8341 accataaagt tcagtcaacg ccgttttgct accccaggca tagttttgca ctgagttaat
     8401 gagtttttgc atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag
     8461 aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa
     8521 aaactgtgta ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca
     8581 taaccaaacc aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcagtct
     8641 ccttttccac tcaaaaaaaa tgatactgag tattacctgc taaccagtga acacgttagc
     8701 gtatctgaat ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactctgttg
     8761 gcgcgtcagg cgtttcatga tgcgtcgttt atgctgcgtc cggctcacca acaacaggtg
     8821 gccgacattc tgcgtgaccc ggaggccagc gaaaatgata aatatgtggc gctgcaattc
     8881 ctgcgtaact ccgacatcgc ggcgaaaggc gttctgccaa cctgtcagga taccggcacc
     8941 gcgattattg ttggtaaaaa agggcagcgt gtatggaccg gtggtggtga tgaagcggcg
     9001 ctggcgcgcg gtgtctataa cacttatatc gaagataatc tgcgctactc gcaaaacgcg
     9061 ccgctggata tgtataaaga agtgaatacc ggcaccaatc tgccagcgca gatcgatctt
     9121 tatgccgttg atggcgacga atacaaattc ctctgtatcg caaaaggtgg tggttcggca
     9181 aacaagacgt atctctatca ggaaaccaaa gcgttactga cgccggggaa actgaaaaat
     9241 tacctggttg agaagatgcg cacgctgggt accgcggcct gtcctccgta tcatattgcg
     9301 ttcgttattg gtggaacttc tgcagaaacg aaccttaaaa cggtgaaact ggcttccgcg
     9361 aaatactatg atgaactgcc aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg
     9421 gaactggaaa aagaattact gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt
     9481 ggtaaatact tcgctcacga catccgcgtg attcgcctgc cacgtcacgg cgcatcctgt
     9541 ccggttggta tgggtgtttc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt
     9601 caggggatct ggatcgaaaa actggaacat aatccaggta aatatatccc tgaagagctg
     9661 cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc
     9721 ctcgcacagt tgtcgcagta tcccgtttct acacgcttat cgcttaacgg cacgattatc
     9781 gttggtcgtg atattgctca cgccaaactg aaagagcgga tggataacgg tgaagggctg
     9841 ccgcaataca ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt
     9901 tatgcctccg gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa
     9961 ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg
    10021 acggatgcct gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggccgct
    10081 gtattggcgc agggaagtat taagagcctg gaatgtgttg aatatccgga actgggaatg
    10141 gaagccatct ggaaaattga agtggaagat ttcccggcgt ttatccttgt ggatgataaa
    10201 ggaaatgact tcttccagca gatacaactc acacagtgca ctcgctgtgt gaaataaaca
    10261 gagccgccct tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa
    10321 tagtaaataa tgagtgagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta
    10381 atcaggtgag gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggggcgat
    10441 tgatgtcccg gcagataagc tgtggggcgc acaaactcaa cgctcgctgg agcatttccg
    10501 catttcgacg gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc
    10561 agcggcaaaa gttaatgaag atttagactt gttgtctgaa gagaaagcga gcgccattcg
    10621 gcaggcggcg gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg
    10681 gcagaccggc tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc
    10741 cagtgaatta ctcggcggtg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt
    10801 gaacaaaagc caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcgctgct
    10861 ggcactgcgc aagcaactca ttccgcagct taaaaccctg acacagacgc tgagtgaaaa
    10921 atcgcgtgca tttgccgata tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc
    10981 gctaacacta gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctcaaaca
    11041 tatcgaatac agcctgcctc acgtagcgga actggctctt ggcggtacag cggtgggtac
    11101 tggactaaat acccatccgg agtatgcgcg tcgcgtagca gatgaactgg cagtcattac
    11161 ctgtgcaccg tttgttaccg cgccgaacaa atttgaagcg cttgcgacct gtgatgccct
    11221 ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga
    11281 tgtccgctgg ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa
    11341 tgagccgggc agctcaatca tgccggggaa agtgaaccca acccagtgtg aagcattaac
    11401 catgctctgc tgtcaggtaa tggggaacga cgtggcgatc aacatgggtg gcgcttccgg
    11461 taactttgaa ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg
    11521 cttgctggca gatggcatgg aaagttttaa caaacactgc gcagtgggta ttgaaccgaa
    11581 tcgtgagcga atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac
    11641 ccacattggt tatgacaaag ccgcggagat cgccaaaaaa gcgcataaag aagggctgac
    11701 cttaaaagct gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt
    11761 acggccagaa cagatggtcg gcagtatgaa agccgggcgt taatctgcaa catacaggtg
    11821 cagccgtgga atgatcaaac gaagcggctg cgcctgaggt ttataacggt gctgcacatt
    11881 gtcggcatcg taatttaaca actcaccaac gtccggcacg cccgcgccat tatcccgatt
    11941 aatcagtgca atcagtggtg tagggcaggc gtgttggact tgtttttgat ctcctttgta
    12001 ccagacgcgg gcaatcggct gcaccttcac tggacgtttg atttttaact tcgcgttctg
    12061 tggcagggca gcgatatcct gatactctcg ctccagtttt ctttgccact cctcgcgcgt
    12121 ccacggtgcg acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc
    12181 acgatgcaaa ttcttaatga tatgtttatt agcccaacca aagcgtaaag tggtggggtc
    12241 gtgcagaacg gtgagcgtgc ggtaagcatt aagggtgatc agccccggca aatgacgatg
    12301 cacccattca aaacgtgccg cggtggggag ttctgattca accgtgacga tatgctcgaa
    12361 cgtggtcttg agtttattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt
    12421 atcgacctgg taacacaaca cgccaggcag acggacagcg gccttgctgc tgcgattttc
    12481 ggactgttgt tgaataaata aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt
    12541 gccgagatgt tgttttacct caatacgatt aagcggatta tgctcatcct ctttttttac
    12601 ctccggcaaa gagaacacgc gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa
    12661 tgtagccagc tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta
    12721 acgcgccata atattaacca ctttagttac aacatactta ttttatagca cagtcgtggt
    12781 ggcttgacca tcgtgcgcgt agtgtcaggc agaggtaaat tgcgggatgt tatgccataa
    12841 cggccagcta aacgagaagc gagcaccacc cagttcgctg atgtcacaat taaccgtacc
    12901 gcccattgcc agtgctattg agtggacaat tgccagcccc agcccgcagc cgccggttga
    12961 gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg
    13021 ggcaatccct gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag
    13081 gctggtttca accgttgaat ggcagtagcg cagggcgtta ttgagcaaat tatccagcac
    13141 gcgctccatt aagcgcatat ccaacgccgc ataatggcct tgcatgagcg tttttatccg
    13201 taccgtttta tcgggcgtta ctgcctgaat atctgccaga tgcgttgaca gccacaacgg
    13261 caggtctggt tcgctaagat gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag
    13321 cagctcttca attaaagctt caagttgact gatatcacga ttcaacgcct gggattcggc
    13381 ggcgctcagg ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag
    13441 ttcgtgagcg ataccgtcaa taagctgttt tttgctggca attaaggcgt tgatattgtc
    13501 cgccatctgg ttaaatgcga taccaagtcg ttcaaagctc gaaccctcat caaagtggat
    13561 acgttcattg agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc
    13621 ctgccagtgc ggacgcatcc agataaacac cggaaaggcg agggaaatag caataaaagc
    13681 gatcagggcg atatccagca atcgcatttg atggaggtaa taaagataag gaacaggacc
    13741 aactgccagc acgtagtggc tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag
    13801 ggcgacaatt tcgccgccac gcagtcggtg catggaaata tcatcaagat ggtatttact
    13861 cagtggctcg acacgcagat cgaaagagag atttaaatcc atctctttca gcgtcttacc
    13921 ccagtcgtgt ggggggatct ctcgcaattc gctgcgcatc agatacagcg aactgttcat
    13981 caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac
    14041 cagcagagac atcacaagga agcagacaaa caataacagg taaaactgga taaacaattt
    14101 tttcattggc atcgcccgca acagaatact attcgtatta attcacgact gtggagcaat
    14161 gtaaaccgcc aactggataa gaccttgttt ggaagataat gtccggaaca gacggatgta
    14221 tccccggtgt attcatattc agtaaattca attttacacc ggggcttgtt tattatgcta
    14281 aggtttaatt taacgtgacg ctttgttgtt ctttagtctc tctatcctta agaagacggg
    14341 ctgatattac accggacatt attgaaccat tcacattaag cgctgttctt gccatatcaa
    14401 cgatcggctc tatggcaata aaaataacta ctaatgcgac agggaaatta agtgttgaaa
    14461 ggacaatcag cgccgcgaat gtcccaccac tacctacgcc tgctacccct attgagccaa
    14521 gcgcgatggc aggtaaaaga gttgcgagga attgcaatga gaagggatcg atacccattg
    14581 ttggagcaat catcgccacc atcacggcag gataaatccc agcgcagcca ttttgtccca
    14641 tgctggaacc aaaagacgct gcaatattag caatcgtatt tggaaccccc aatttttcca
    14701 atgcagaaat agccagagga attgaagccg cactactgcg agaaacaaac gcaaaggtaa
    14761 gtacttgcca gacggtcttg taatagttaa cggggttttt acctattaac agcaaaatgc
    14821 ccgagtgaac aataaacatc acaaaaacgg ccagataaca ggcgacgatg aacccgagca
    14881 aattggtaaa ctgtgaaagc tgatacgttg agaagacttt cgccattagt gccattaccc
    14941 cataaggcgt cagcgcaatg acaatacgga ccattttcat tacccatatt tgaatcgcgt
    15001 tgatccccgc gctcaatttt tcgccctctg ctggtgcatc tttctttact tttaacagcg
    15061 caataccagc aagaattgta aagatgacga tgccaataac cgaaacactt cttgccccgg
    15121 tcagatcaag gaagaggttg gttgggatca atgacgtaat gagtcgcggt aaagataccg
    15181 cagctgtttt cgagacgtca tcgctggtta aagaggattg catatgacca aaggcgctgg
    15241 catcaaggcc aattgcactg gcaatagcca ggccaactaa acctgcaatc atgactaaac
    15301 acaacataca ggcaacaata gttaaagaca tcttgccaat accagcgcta ttttcgagct
    15361 tattaatcgc tgaaagaatc gaaatgaata tgagtggaac ggcaacaagt tttaataaat
    15421 taacataacc gttaccagca atattatacc attcgataac ggtttcactg cttgtctcag
    15481 gaatgaaatg cagggagata ccaatgatag cccccaggaa aatcgcgata aaaacacttt
    15541 tggaaaggga ttcgctcttt tgatccgttt tgaaaatccc ccatgagcag atcaaaaaaa
    15601 gcacaatata tgtgaaagtt gtaaccgttt tttaagcctc ttgcctcatt cagcacctga
    15661 aatgccggaa agagtttgtt gtaaaaaatc tgcgccataa gacgcaggga cgaggcgatt
    15721 atagagaaat tttttctcat tttcatatca aatgtagggg gtttggaatt tgaataagct
    15781 cttattaatt gaattcgtta attgttctct ttttcagatg aacaaatatt agtcatctga
    15841 ttgtttattg tcactatccc atgcatgagg cgcaaaaaga tagcctttgt tacgcacagt
    15901 tttaatgcga taaggttctg cggcgttatc gagcagtttt tttcttaacc gcgaaatagc
    15961 cacgtccacg ctacgatcca gtccgtcata actgacgccg cgtaaatttt tcagcaatgc
    16021 atcgcggtcc atgatttgcc cggcatgcgt agctagttcc cacaataatt cgaaatcagc
    16081 tgtcgagagc gagatttcag tgttagccag ggtgactacg cggttgatgg ggtcgatagt
    16141 taacgtgccg aaatgcaggg ctttgtaggg agtaagagac gtttcctgaa gacctttggt
    16201 cagtgtggct tgctcattct gacgcaaatg caaacgtaaa cgcgctagca aaacagcagg
    16261 gggcgtcgtt ttgagaatat agtcgcaggc acccatttcc agtgccagga tgtggttcat
    16321 atcgctatcg agagaggtta gaagaacaat cggtccagac cactttgcgc gtaaatcacg
    16381 acaaatggtc atgccgtcct tgcctggtag catgatgtcg agtaacacca aatccggatt
    16441 ttctcgcaaa atggtttctt cggcctggtc gccgcgcggt tctacggtaa cctgcatatc
    16501 atgttttgcc aggtacgcgg caatcagtga accgacttcc gcatcatctt ccacaaatac
    16561 gatagtgttc ataacattca cggtagatat aaaaacgtca caatacaccg cgctgttttt
    16621 acttactatc aatcgtttct aaacaagttt tcatcaggta ttctactgat gacttgttat
    16681 ggaagtgtta aggtaaaaag atggggctgg taatcaaagc cgcgctcggt gcgctggtag
    16741 tgctgttgat tggtgtttta gcaaaaacga aaaattatta tatcgccggg ctgattccac
    16801 ttttcccgac ctttgcgctt atcgcgcatt atattgttgc cagcgaacgc ggcattgaag
    16861 ccttacgcgc aaccatcatt tttagtatgt ggtcgattat tccctatttt gtttacctgg
    16921 tgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc cgcttttgtt ggttctgttg
    16981 cgtgttgggg gataagcgca tgggtgttga ttatatgctg gataaagctg cattaacgca
    17041 gatgacgacc gccatcaagt gggaaactgc gtccggtgac aaagcaactg gtaagtaagt
    17101 aatcgaccag gtcgatcact tctttctcgc caggcgcggt tttcatcagt gatttattca
    17161 gcgcctgttg tcgatattcg gcatcatcat gttcattaaa caggatcagc gatggcgcaa
    17221 tagaattcac tttcacttcc ggtgccagct tgcgggcaaa cgagcgggtc atattatcca
    17281 gtgccgcttt gcttgcagca tacgcaacat gtttgtcgct accgcgctcc accacataat
    17341 cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc acgcagtaat ctttccagcg
    17401 catggttgag caggtatggg gtattaacgt ggatctgcat catgcaagcc agtacgtcgg
    17461 ccagtggcgc acccggtttt tccgcaatcc acgcactggc gttatgcaaa atagcacgca
    17521 gaccatgggt ggtttttagt acttcatcgg caaacgccat cacaccgtcg ttggtcgaaa
    17581 aatcagcctg aatacactgc gcacctgcat taatcagtcc atcaatggct ggatagtgtg
    17641 tccgatagct gacaatcacc ggttgctttt gattaatgaa atgccatgcg agggcgaggc
    17701 cgatgcgacg acctccgcca gtaattaata ttggcaaggg ctgggtttta cccatcgtta
    17761 tctcctttgc tatccaacga tgggatgaac gttatcccac cagcatccag gtcgccggaa
    17821 ctgaggcaat taacagcatg ccaatcagca ccatttcctg acggttcagt acattatcat
    17881 gtgtatgcgt tttgcgcgca tacaggaaaa ccagcagccc cggtgcatac agaacaacgg
    17941 ataacagcaa gtgcattgga ccagaggcgt acaataacca taagccataa atgcaggcac
    18001 cgacacctac cgctttatgc agtggacggg ttgcgatttt cagcaagaac gcgccgacca
    18061 ggaaataggg caccagaatc atttctgagg cgatggtcag caacgtgttg taatcggaac
    18121 cggtcagcca gatgagtacc agacaaattt gcacgcagat gttggtcaac cacagtgagg
    18181 cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg tgggaatgct ttatgagtgg
    18241 cagccaggaa cggaacttct gccgccatga tggtccagct taagtacgcg ccgcaaacgg
    18301 aaacgatcaa accggcagcg atgatgattt cgccccatgg tcccatcatt tccaccatca
    18361 gaccggccat tgacgggtta cgaatttcag ccagttcagg acgtgccacc acacccagcg
    18421 aaagcagcgt taccagcaag taaacgccca gagcggagag aaccgccagc agtgtcgctt
    18481 tgccaacatc acgtttatta cgcgcacgcg cagaaacaac cacggcacct tccacaccaa
    18541 tgaaaaccca cagggtgatc agcatggtgt ttttcacctg ttcccaaaca ggtacgccaa
    18601 gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt gaacatcatc atcgccagca
    18661 caacaaacag acccagcggc aacaattttg ccagtgtcgc caccaggtta atgctggcag
    18721 cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca caataaagcc gatgcaccga
    18781 ctatcgattg ccaggtatta ccatcgccaa acaggcgcaa ttccggcgtg tccgtaaaaa
    18841 agcttaacgc ggaaaaaacg atcaccagat aggagacgtt ggcgatgacc gcgcacagcc
    18901 agtatcccca tgcggaacaa aagccgatta gctcgccaaa cccttcgcgg gcataggtaa
    18961 agataccgcc atcaagttcg ggacgaatgc gcgtgaggat cagcatggca aaggccagca
    19021 ataaaatgcc agcgccagta ataccccagc cgatgagcag tgctgccggg ctggcaactg
    19081 ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag cattgagctt aataccagcg
    19141 cggtgagtgc gctcagtccc agtttctttt ccatcgatat ccagtgatta aaacgtgata
    19201 agaagaataa atattagggc aaaacgcata aaaatggagt ttgccactga agcgcgggat
    19261 tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa atttgtgact aaaaatgcga
    19321 aaggcggcat caagccgcct tataggagta actgaatagt tatttataca gatctgcgct
    19381 gacggtcagg ttattaccac gtttctgcca ctggcgggtg atgtggtaat acttagcacc
    19441 tttcttcgcg gcacgtttcg caacctgata ggagacttcg gtcatgttgc cgtagttgcc
    19501 agagaatttg atgctgtcga acgggaccat catcgctgct gtcgctttgt tcagttcttc
    19561 gactttagtg ccatccggga gcgtgacggt gtaacgcccg ccttttgatg actgggtttc
    19621 aaagaagcga ccgacttcag aacttggtga tgcggtagtc gcaacacccg ggatctcaac
    19681 tttcttcgcg gcttcgccac cggcagccag agctgcacgt cctgcttcgg aatctgccgg
    19741 gatcacatcc gggctctgga cgatacgttt cttagcatct tttttataga tgaatgcagt
    19801 aatacgctgg ttgccgccct ggttggcatc gatttgacga acgatgtaga aagagtaggc
    19861 acctttcgct tttgccgctt tggtgatggc atcattgact tctggctggc tacgatagaa
    19921 gccctggacg gtgaccgtgt caaacggttc aatcagaaca gcctgatctt tcggcagttc
    19981 gacaacaccg ttaattacgc gattacttgt ttcttctgct ttttcagcat cggctttata
    20041 gaggtcagcg accacacgcc agttaccgct gttaccaaaa tcagaagtgt cgacaacata
    20101 aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg gctttcaccg cttcgccaat
    20161 agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt ttaaccgctg ccgcttgctc
    20221 cggtgtcagt tctgttgctg cgttaacgga gaatgccgta gcagaaagca gtgccgacgc
    20281 caggagggtg ttcttaagct tcataaaaat aatccttcgc cttgcgcaaa ccaggtactg
    20341 gtattgttat taacgagaaa cgtggctgat tattgcattt aaacggtgta actgtctgcg
    20401 tcatttttca tatcacattc cttaaaccaa ttttaatcct gctcaaatga ccgtctatgc
    20461 ttaaaaaaca gccgtatcag catcattact actgaagcaa ctgaattgta taagttaatt
    20521 taatgttaag tagtgattcg tgccggggcg atgtctcgtt ttacccgacc gtcgaagaca
    20581 attatcagtc tttatccggc gttctaaggt gtttatccca ctatcacggc tgaatcgtta
    20641 atattttgcg agttcacgcc gaaatactga tttttggcgc tagatcacag gcataatttt
    20701 cagtacgtta tagggcgttt gttactaatt tattttaacg gagtaacatt tagctcgtac
    20761 atgagcagct tgtgtggctc ctgacacagg caaaccatca tcaataaaac cgatggaagg
    20821 gaatatcatg cgaattggca taccaagaga acggttaacc aatgaaaccc gtgttgcagc
    20881 aacgccaaaa acagtggaac agctgctgaa actgggtttt accgtcgcgg tagagagcgg
    20941 cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg caagcgggcg ctgaaattgt
    21001 agaagggaat agcgtctggc agtcagagat cattctgaag gtcaatgcgc cgttagatga
    21061 tgaaattgcg ttactgaatc ccgggacaac gctggtgagt tttatctggc ctgcgcagaa
    21121 tccggaatta atgcaaaaac ttgcggaacg taacgtgacc gtgatggcga tggactctgt
    21181 gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc tcgatggcga atatcgccgg
    21241 ttatcgcgcc attgttgaag cggcacatga atttgggcgc ttctttaccg ggcaaatcac
    21301 tgcggccggg aaagtgccac cggcaaaagt gatggtgatt ggtgccggtg ttgcaggttt
    21361 ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg cgtgcattcg acacccgccc
    21421 ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc ctcgagctgg attttaaaga
    21481 ggaagcgggc agcggcgatg gctatgccaa agtgatgtcg gacgcgttca tcaaagcgga
    21541 aatggaactc tttgccgccc aggcaaaaga ggtcgatatc attgtcacca ccgcgcttat
    21601 tccaggcaaa ccagcgccga agctaattac ccgtgaaatg gttgactcca tgaaggcggg
    21661 cagtgtgatt gtcgacctgg cagcccaaaa cggtggcaac tgtgaataca tcgtgccggg
    21721 tgaaatcttc agtacggaaa atggtgtcaa agtgattggt tataccgatc ttccgggccg
    21781 tctgccgacg caatcctcac agctttacgg tactaacctc gttaatctgc tgaaactgtt
    21841 gtgcaaagag aaagacggca atatcactgt tgattttgat gatgtggtga ttcgcggcgt
    21901 gaccgtgatc cgtgcgggcg aaattacctg gccggcaccg ccgattcagg tatcagctca
    21961 gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact gaggaaaaat gtgcctgctc
    22021 accgtggcgt aaatacgcgt tgatggcgct ggcaatcatc ctgttcggtt ggctgtcaag
    22081 cgttgcgccg aaagagtttc ttgggcactt caccgttttc gcgctggcct gcgttgtcgg
    22141 ttattacgtg gtgtggaatg tatcgcacgc gctgcataca ccgttgatgt cggttaccaa
    22201 cgcgatttca gggattattg ttgtcggagc actgttgcag attggccagg gaggctgggt
    22261 tagcttcctt agttttatcg cggtgcttat agccagcatt aatattttcg gtggcttcac
    22321 cgtgactcag cgcatgctga aaatgttccg caaaaattaa ggggtaacat atgtctggag
    22381 gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt ctggccggtc
    22441 tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg atggcgattg
    22501 cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc ttgctggcga
    22561 tggtcattgg tggggcaatt ggtatccggc tggcgaagaa agttgaaatg accgaaatgc
    22621 cagaactggt ggcgatcctg cacagcttcg tgggtctggc ggcagtgctg gttggcttta
    22681 acagttatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt cacctgacgg
    22741 aagtgttcct cggtatcttc atcggggcgg taacgttcac gggctcggtg gtggcgttcg
    22801 gcaaactgtg tggcaagatt tcgtctaaac cgttgatgct gccaaaccgt cacaaaatga
    22861 acctggcggc tctggtcgtt tccttcttgc tgctgattgt atttgttcgc acggacagcg
    22921 tcggcctgca agtgctggca ttgctgataa tgaccgccat tgcgctggta ttcggctggc
    22981 atttagtcgc ctccatcggt ggtgcagata tgccggtggt ggtgtcgatg ctgaactcgt
    23041 actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg ctgattgtga
    23101 ccggtgcgct ggtcggttct tcgggggcta tcctttctta cattatgtgt aaggcgatga
    23161 accgttcctt tatcagcgtt attgcgggtg gtttcggcac cgacggctct tctactggcg
    23221 atgatcagga agtgggtgag caccgcgaaa tcaccgcaga agagacagcg gaactgctga
    23281 aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg caggcgcaat
    23341 atcctgtcgc tgaaattacc gagaaactcc gcgctcgtgg tattaatgtg cgtttcggta
    23401 tccacccggt tgcggggcgt ttgcctggac atatgaacgt attgctggct gaagcaaaag
    23461 taccgtatga catcgtgctg gaaatggacg agatcaacga tgactttgct gataccgata
    23521 ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat gatccgaaga
    23581 gtccgattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg attgtcttta
    23641 aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc aaggaaaaca
    23701 cccacatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa gctctgtaac
    23761 cctgacggcc tctgcggagg ccgtcactct ttattgagat cgcttaacag aacggcgatg
    23821 ctttgacctc ccgctgtttg ttcaagcgca attttgacaa taattgtcaa cggcacggaa
    23881 agcagcatac ccaccggtcc taacaaccat ccccaaaaaa tcaacgacaa aaataccacc
    23941 aatgtcgaaa gccccagccc acgtcccatg atgcgcggct ccagaatatt gccgaagacc
    24001 agattaatca gcagatatcc cgccagcacc agcaacgctt cgtagaagcc attaaacacc
    24061 agtacctgag cgataggggg gattgccgcg agaactgaac caatattagg gatgtaatta
    24121 agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa catcgagtgc ggcgagcatc
    24181 gcccaggcga ccaggccggt gatgatgctg atggctgttt tcagcaccag ataatgagaa
    24241 acactgtcaa tcgcacgttg aatcgccgcc atcccttcaa ccggacgcgc catcatttgc
    24301 tgaaattttc cgggcaattg tggcacttcg agcagcataa acagcaccgt cagcagcaat
    24361 aaaaatattg atgacatggc attagataac tgcgtcaata agttggtgag caacgtcatc
    24421 gccgcgttcg gatcaatgta atgcgccagt tggtcaactg agacgtcaat ccctacgcgt
    24481 tgcaacaacg gctcaagagc ttgcagcggc gtcataatag agttgcgata ttgcggtaac
    24541 gtccacgtca actcgttgag cgcggaaccc agataagcta atagcaacac catcgccatc
    24601 acgatgattg tcatcaaaat cgacactgcc agtacacgtg gcacacgcca gcggaccata
    24661 tgttgcacca gcgggttaag aataacagca ataaataatg cgagaataaa cggcacgatg
    24721 atctcggcgg caaaacggat accgcagaga ataatgacca gcattcccaa cataatgacg
    24781 atttttaggc cattgagcgt gatgatcggc tttgccatgc ttgtccccgt ttttcttcct
    24841 ttgctaataa taatgtcttt ttcgctccgt catcataacg aaaatcaaac cgctcaggta
    24901 aagaagtgaa aatattttga gttaattctt aagctatgat acaaatcagg cgtgttcaac
    24961 taccgaggac aattatcatc tgcgatgacg agaagcaaca ctgcggataa ttgtaatatt
    25021 atggacaatt tgttcaggac gttattttct acatttaccc atcttcgtac ctcatccact
    25081 atcttgctcg taggtgagca gcactggcgc aacgcgcttt agtcctttct tctgcctaag
    25141 cttgccctat gcgcagggct tgaccacttt actttggttt cgctgaatta agcgaaaatt
    25201 aaaatagttc tcttgcagga gaaggacaat gtatatttat tggattttat taggtctggc
    25261 tattgctaca gaaattaccg gtacgctgtc aatgaaatgg gcgagcgtca gtgagggaaa
    25321 tggcggcttt attttaatgc tggtgatgat ttctctgtcg tatatatttc tctctttcgc
    25381 cgttaaaaaa atcgccttag gcgtagctta tgcgctgtgg gaaggtatcg gtattttatt
    25441 tattaccttg tttagcgttt tgttattcga cgaaagttta tcgctgatga aaattgccgg
    25501 gttaaccacc ctggtcgccg ggatagtgtt gatcaaatca ggtacccgta aagcgcgtaa
    25561 acctgaactg gaggtgaacc atggcgcagt ttgaatgggt tcacgccgcc tggctggcat
    25621 tggcaatcgt gctggaaatc gttgctaacg tctttttgaa attttctgac ggctttcgtc
    25681 gcaaaatatt tggcttgctc tccctggcgg cggtgctggc ggcctttagt gcgctttctc
    25741 aggccgttaa agggatcgac ttgtctgtcg tttatgcatt gtggggcggg tttggtattg
    25801 ccgccacgtt agccgcaggt tggatcttgt ttggtcaacg gttaaatcgt aaaggctgga
    25861 ttggcctggt cttgctgttg gctggaatga tcatggtgaa acttgcctga tgaagacgct
    25921 gcccgcgctg tcgggcagcg tttgaacatt atttttgcga cagttgatcc agcttgtcgc
    25981 gaaaaccggt aacagaaatg gcccggttat cggcgcgcca gcgatctttc gcggcaggag
    26041 ccgaactttg caccccaatt aattgccagc cgtcatcggt atgcaacatc agaggcgaac
    26101 cgctgtcacc cggcaaggta tcgcactgat gtgacatcac cgacgtttgc gcccagccag
    26161 tcacttcaca gttttgatga ctgtataacg tatcgagatg atcttcaggg tatcccgcct
    26221 gagtcacttt acgaccagcc gattttaatg cggcagtaag cgcggcttta tctccctcaa
    26281 ataacggcaa cggtgtaatg ccagaagggg gattacgtag cacaatcaat ccgaagtccc
    26341 acggtgcagc tgcgggagga acaatccaac catccccatc tgcttttaac cgctttccca
    26401 gtgtcggatc aacgcggcct tctatgtcgt ggatctcata gcgccaaaga cctttatttg
    26461 acacaaaacg cagcgctacc gccttatccg ctttaccctt aggcggcgtc aataaacagt
    26521 gtcctgccgt taatgccaga ttgggtgcaa tcagcgtcgc cgtacataaa ttgccgctgg
    26581 ccgtttccag ttgcccaacc gcatcccacg gtgattgggt cgtgtcattc actggcacac
    26641 gatcatcatg accaaaaaac agggtgctga cctcatcgtt tgccgatttg gcaacgtctg
    26701 gtttatctgc aaacacaaaa gcagacgtca aactaattgc acccaacact acagcaatgg
    26761 ttgtacgcat atcacactct ggtgggtaat tatgattatt aaaagcaaac cctcataaat
    26821 actatagacg ggacgggatg aaagtgggag taaaatcaga taactacagt caggaaagat
    26881 aaaaacgggc ggcaatcagt gcgcataaaa taaggatgat aagaatgaac tcaaagcgat
    26941 aacggcccac catactaccc tccgctaaag gcggtgctaa acgttacccg cttagcaccg
    27001 taattttcag gcgcgagggg gcgcgccagc attactgttg aaaacttacg ctgcgggttg
    27061 tgcagcaggt tttgccggtt gctgatggcc gtgtttcccg gcgtgtttct tcgctgcctg
    27121 cgctttttgt tcaggggctt tctgttcagc tttcgtattt ttatgatgct ttttagccgc
    27181 ctgcgctttc tgggcaggtg ctgctttatg ctgtttttta tgatgtgtag ttttcgctgg
    27241 cgctgctttg gtggtcgtcg cagtcggagc aggtgtggtc gcagtctctg cagcaaaggc
    27301 ggcagaagac agacccatag cagcggcaac aaccagagct aatacttttt tcattgtcat
    27361 accctcaatt tgttttttca tttaacccca ctgcggggcc gttgaaataa ctatatccct
    27421 gggaatggca gacttccgtg agtggttggt ttcagcgtgt aacgatatgt acaaacgctg
    27481 aataaattac agcgttgata ataacgtatt gtgacttaag gaaaatttag ctaccaataa
    27541 tagtagtctt gatcgggtta actattatac tacttacaaa gcggtagaat aactgcgcat
    27601 cagtaataaa tgacacacag caaaatgaat ccgtttattt gggtacttat aatcctgatg
    27661 acgctagatg cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg
    27721 ttggtttgag caggcaataa gtccggacgg gtatttaccg cagtccggac ttatttttca
    27781 ggcgtgcaga cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc
    27841 aataaatgcg ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc
    27901 ggcaatactc gaccccagat agtaactgaa cagatacagc gaggaggcct agcctttagc
    27961 gcgttttgcg cgggggccga tccagctgct ggctactgag tgggctgcga agaatccggc
    28021 tgagaagagt aacattccgg caaagatcag ccacagcgag ctaaataagg tcatcagtaa
    28081 accaaacggc ataactcccg tcgaaaacaa catcaccgga ccgcgcccat agcgggtggt
    28141 catggttccg gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc
    28201 aaccacggcc tgactgacat gctaggggga aagcatcaac cgatagccga tgtaattaaa
    28261 cagcgtgacg aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg
    28321 gtcacgccag tgcagacgaa agttgataaa taacgtctta gggcgcagcg aagtcgggca
    28381 aaaatggcgt gattcaggga ggattttcca gaacatcaac gccgaggcca gcgcgaaaca
    28441 accgattgcc gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa
    28501 gcgtccgctc atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac
    28561 gaaactgggg tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa
    28621 cgaaagccca atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga
    28681 aagtaacgta caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat
    28741 ggcatcggat agcgggccag taaatagcaa accgatagcc aacatcgccg tggaaatgga
    28801 cagtgaaata ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat
    28861 aggctgcaca caatagagaa gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt
    28921 gacgcgcata aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg
    28981 cttgtcagtg tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc
    29041 ttgctaaata tgcctgtgga tcaggcttat acatagggta ggaaaatcga attgttctgt
    29101 ctaatatatt aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct
    29161 gcgttacttt gttgctgttg cggaagagct gcattttggg cgcgcggctg cccgcctgaa
    29221 tatttcgcaa ccgccgctaa gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg
    29281 actgctggca cgaactaatc gcagtgtatt gctgacggca gcaggaaaac agtttcttgc
    29341 agatagtcgg caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg aaagactgca
    29401 tcagggtgaa gcgggggagt tgcgtattgg ttttacttcg tccgctcctt ttattcgggc
    29461 ggtgtccgat acgttatcgc tgtttcgccg tgattatcct gatgtccatt tacaaacccg
    29521 cgaaatgaac actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt
    29581 gctgcgtaac acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct
    29641 tatggcgatg atcccgtacg atcatcccct ggcaaataat ccgagtgtaa cactggctga
    29701 actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg ggactgggct
    29761 gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca tcactcagga
    29821 ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg tttcaatttt
    29881 gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga
    29941 ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccagctgc
    30001 gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag
    30061 cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt
    30121 gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt
    30181 gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg cgcggtttat
    30241 cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcagctg
    30301 gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcgagag
    30361 ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt ggttgaaact
    30421 gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct tgctctgcgc
    30481 gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa agatgacttg
    30541 ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa
    30601 cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac ggaaaatggt
    30661 attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggcgctg
    30721 gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg gacgatggca
    30781 gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac
    30841 aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag cagtagtctc
    30901 gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg cgggaaccac
    30961 ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca gctgcgtctc
    31021 aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc attgtgtcag
    31081 gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg cgcgcatgta
    31141 gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca
    31201 ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat ccgtcagcag
    31261 gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc
    31321 acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt gttgattcgt
    31381 ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg
    31441 ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat atttgtgaag
    31501 cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt
    31561 agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaagggt
    31621 tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa
    31681 agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc
    31741 tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct
    31801 catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg tagaagggac
    31861 tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg tagtgcagga
    31921 acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct
    31981 aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat
    32041 caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa aacttccggc
    32101 accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata
    32161 catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtcc
    32221 gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc
    32281 cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt gccgccaaca
    32341 gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca gggaaccata
    32401 atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa
    32461 agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt ttacaaagga
    32521 agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt acggtgctat
    32581 agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc aacccgacta
    32641 gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga caggcgttca
    32701 ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg
    32761 catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac gcgtcgctat
    32821 gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc gggccgagag
    32881 aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca gcaagcgggg
    32941 cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat ttccattcct
    33001 ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa agaatcatcg
    33061 caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca agcgatttaa
    33121 ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg taatcggtcg
    33181 gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa cccgccgcca
    33241 gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc gctgcattga
    33301 ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc gcatgacgaa
    33361 acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc agacggcgga
    33421 acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat gggcaatgaa
    33481 acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg tgctttaaga
    33541 gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gcgcgtaatc gtcaccatcc
    33601 ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa tcaacagttg
    33661 tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt agaaagggtg
    33721 ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa ggtgccatgc
    33781 cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag ccatcaacag
    33841 cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc
    33901 gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt cgcgatccag
    33961 ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca aacgctgcca
    34021 ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga
    34081 cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg caaccagagg
    34141 tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac
    34201 agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat gtgcgggttg
    34261 ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca ggccataaaa
    34321 gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa caagtccggc
    34381 aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac ctgcgcataa
    34441 caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc tggcttgttg
    34501 cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct cggcacaagc
    34561 gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc
    34621 ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga agaaagccag
    34681 cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc aggtcatcat
    34741 ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc ccaacgcttg
    34801 cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa aaaatatgga
    34861 accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg agttaaacgc
    34921 tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca tcaacagcca
    34981 gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg cggcgaacca
    35041 gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg taaagataac
    35101 cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta gccagataac
    35161 cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata ttgggttttg
    35221 tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc tggcgcagct
    35281 cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat atgggttgtt
    35341 tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct ttttcagcat
    35401 tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg atacaaacat
    35461 cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg gggcagactt
    35521 ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa gcgaacacgt
    35581 tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca taaatacggc
    35641 ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg caggttttac
    35701 agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg ggctactcct
    35761 taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc cagcggtgaa
    35821 gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc accaaacata
    35881 tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat acgcggagtc
    35941 acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt atcgccatga
    36001 cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc
    36061 tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg gcgacttcac
    36121 tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt tgggcgagga
    36181 tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc tctgttggca
    36241 tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag tggtcaatgc
    36301 caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga ttgatgtcgc
    36361 catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg gggacatcca
    36421 gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca tggtcgatgg
    36481 catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc atcgggaacc
    36541 attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg tttatgccga
    36601 cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg ccgttggagt
    36661 gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg atctctcgtg
    36721 ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat tccggcgttt
    36781 tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg ttacgtggtg
    36841 cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga aatggctgat
    36901 cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg ccatcggtgc
    36961 cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta tagcgtggat
    37021 cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag taagtgacac
    37081 caccgccgct catccgcgtt tctgccgggt gatttccgaa cataacgacc agtttcgtat
    37141 tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc attgcggcac
    37201 tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa gagttcatca
    37261 gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt ccgtacagga
    37321 catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca ctgatggtat
    37381 ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg ccgacgcgct
    37441 tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag cgcccgcgta
    37501 aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca gactccaccc
    37561 agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg cagttaacgg
    37621 tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg accatttggg
    37681 aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg gaggttttca
    37741 tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg attttcatca
    37801 ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt gtagtgggtt
    37861 atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc agtggtgaag
    37921 ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg ccagacatat
    37981 tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg cgtggtgtta
    38041 ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata tcgccgttgg
    38101 caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg caggctgcct
    38161 tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg aaaccaaaca
    38221 gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt gaggcataaa
    38281 ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca atttccgcca
    38341 gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt gcctcggggt
    38401 cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc ttatagatac
    38461 ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc attttggcgt
    38521 acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga tagacgtcag
    38581 gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt tcgaattttt
    38641 cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag tcgttaggaa
    38701 taatatcttc ctgctcaacg gtcatcaggt ctgccagcag aatgtcggca tattttgccg
    38761 atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg tcctgcagaa
    38821 tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc gcgtagttcc
    38881 agatggactc tactgtcaga cggatggacg gacttgaagt agttgactca acgctactta
    38941 aggtcctgat atgactgaca ttaaccatga cgatactgca tatccatcca gttctttctc
    39001 tgggtctccc tatctgtatc agcaagtcta cacctcaagt cctgacacaa ggctgacaca
    39061 aatcaaggac cttgctacgc cacagctagt ccggagaacc aacgggcgtt acactgtccg
    39121 cttcagactt aaaggagcta ccagcccgtt tctatcagtc tcaacccgca gcacagaccg
    39181 gaggaccgct acaatgcgtc agagagagct cgcagccaca gctaaggcat tcctactgga
    39241 caatcctgaa gtcacactac aggagcttac agcgcacata cggtccatgg ctgagcagtt
    39301 cctgactgat gccagtgatg actactggaa tggcgtagag gtggcgacac tggttgacga
    39361 gaagtccaac ctcagggaac ttgcagcgac aagagcgttg agtgtggacc agcagagggg
    39421 cattaagtta gcgctggaag tcctgaccgc agcacagcag agagtcgaca ctggtgatac
    39481 ctctggtctg attaagctga ttgatggtgg cgtaagccag cttactgatg ataataacca
    39541 tactgattac tcaactatag gcgactctac gtcgattctg aagaatgagc agggggatag
    39601 accagcagtt ttcacacagg aacgtcagtt actcactcca gagtcacgag aatcatctgt
    39661 actttttcag gaattggtga catcactgct ggctgagaag gtccagacac tgaaacaatc
    39721 cagctttaag gacttgtcag cgtccct
//
LOCUS       DAEBXD010000030        38417 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEBXD010000030 DAEBXD010000000
VERSION     DAEBXD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38417
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.030"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..74)
                     /locus_tag="KJL94_001598"
     CDS             complement(<1..74)
                     /locus_tag="KJL94_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBD4409997.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KJL94_001599"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KJL94_001599"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBD4409998.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="KJL94_001600"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="KJL94_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBD4409999.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KJL94_001601"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KJL94_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBD4410000.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="KJL94_001602"
     CDS             2704..2940
                     /locus_tag="KJL94_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBD4410001.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="KJL94_001603"
     CDS             2943..3800
                     /locus_tag="KJL94_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4410002.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5797
                     /locus_tag="KJL94_001604"
                     /pseudo
     CDS             3800..5797
                     /locus_tag="KJL94_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KJL94_001605"
     CDS             complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KJL94_001605"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBD4410003.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6400..7296)
                     /locus_tag="KJL94_001606"
     CDS             complement(6400..7296)
                     /locus_tag="KJL94_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBD4410004.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7345..7584)
                     /locus_tag="KJL94_001607"
     CDS             complement(7345..7584)
                     /locus_tag="KJL94_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBD4410005.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7687..8286
                     /gene="nemR"
                     /locus_tag="KJL94_001608"
     CDS             7687..8286
                     /gene="nemR"
                     /locus_tag="KJL94_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBD4410006.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8323..9420
                     /gene="nemA"
                     /locus_tag="KJL94_001609"
     CDS             8323..9420
                     /gene="nemA"
                     /locus_tag="KJL94_001609"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBD4410007.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9501..9908
                     /gene="gloA"
                     /locus_tag="KJL94_001610"
     CDS             9501..9908
                     /gene="gloA"
                     /locus_tag="KJL94_001610"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBD4410008.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10011..10658
                     /gene="rnt"
                     /locus_tag="KJL94_001611"
     CDS             10011..10658
                     /gene="rnt"
                     /locus_tag="KJL94_001611"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBD4410009.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10751..15209
                     /locus_tag="KJL94_001612"
                     /pseudo
     CDS             10751..15209
                     /locus_tag="KJL94_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15253..16461)
                     /locus_tag="KJL94_001613"
     CDS             complement(15253..16461)
                     /locus_tag="KJL94_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4410010.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16469..16969
                     /gene="tnpA"
                     /locus_tag="KJL94_001614"
     CDS             16469..16969
                     /gene="tnpA"
                     /locus_tag="KJL94_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBD4410011.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KJL94_001615"
     CDS             complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KJL94_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBD4410012.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17690..18517
                     /gene="mepH"
                     /locus_tag="KJL94_001616"
     CDS             17690..18517
                     /gene="mepH"
                     /locus_tag="KJL94_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBD4410013.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18645..19226
                     /gene="sodB"
                     /locus_tag="KJL94_001617"
     CDS             18645..19226
                     /gene="sodB"
                     /locus_tag="KJL94_001617"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBD4410014.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19372..20541)
                     /locus_tag="KJL94_001618"
     CDS             complement(19372..20541)
                     /locus_tag="KJL94_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410015.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KJL94_001619"
     CDS             complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KJL94_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBD4410016.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21095..22120
                     /gene="purR"
                     /locus_tag="KJL94_001620"
     CDS             21095..22120
                     /gene="purR"
                     /locus_tag="KJL94_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBD4410017.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22117..23049)
                     /locus_tag="KJL94_001621"
     CDS             complement(22117..23049)
                     /locus_tag="KJL94_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4410018.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23162..24373
                     /locus_tag="KJL94_001622"
     CDS             23162..24373
                     /locus_tag="KJL94_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBD4410019.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24664..25812
                     /gene="cfa"
                     /locus_tag="KJL94_001623"
     CDS             24664..25812
                     /gene="cfa"
                     /locus_tag="KJL94_001623"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBD4410020.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KJL94_001624"
     CDS             complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KJL94_001624"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBD4410021.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26708..28081
                     /gene="mdtK"
                     /locus_tag="KJL94_001625"
     CDS             26708..28081
                     /gene="mdtK"
                     /locus_tag="KJL94_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBD4410022.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28122..29378)
                     /locus_tag="KJL94_001626"
     CDS             complement(28122..29378)
                     /locus_tag="KJL94_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBD4410023.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29686..29762
                     /locus_tag="KJL94_001627"
     tRNA            29686..29762
                     /locus_tag="KJL94_001627"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29720..29722,aa:Val,seq:gac)
     gene            29767..29843
                     /locus_tag="KJL94_001628"
     tRNA            29767..29843
                     /locus_tag="KJL94_001628"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29801..29803,aa:Val,seq:gac)
     gene            29951..30256
                     /locus_tag="KJL94_001629"
     CDS             29951..30256
                     /locus_tag="KJL94_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBD4410024.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30382..31986
                     /locus_tag="KJL94_001630"
     CDS             30382..31986
                     /locus_tag="KJL94_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBD4410025.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(31998..32810)
                     /locus_tag="KJL94_001631"
     CDS             complement(31998..32810)
                     /locus_tag="KJL94_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410026.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32814..33599)
                     /locus_tag="KJL94_001632"
     CDS             complement(32814..33599)
                     /locus_tag="KJL94_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBD4410027.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33596..34264)
                     /locus_tag="KJL94_001633"
     CDS             complement(33596..34264)
                     /locus_tag="KJL94_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBD4410028.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34328..34966)
                     /locus_tag="KJL94_001634"
     CDS             complement(34328..34966)
                     /locus_tag="KJL94_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBD4410029.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34979..37080)
                     /locus_tag="KJL94_001635"
                     /pseudo
     CDS             complement(34979..37080)
                     /locus_tag="KJL94_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37101..37727)
                     /locus_tag="KJL94_001636"
     CDS             complement(37101..37727)
                     /locus_tag="KJL94_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBD4410030.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KJL94_001637"
     CDS             complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KJL94_001637"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBD4410031.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9261 a        10032 c         9403 g         9721 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttacttttgg tcaaatttgg cctgatggtc cagattagcg atctgtggca
     5101 atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat tgcagatgcc
     5161 caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta tcgctgtcac
     5221 taatcccccg gtgtatgatt ttgctgattt tcttaactat aatctggcaa aaatcgttgg
     5281 cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag
     5341 ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc gccatccaac
     5401 actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt tgagtaacag
     5461 ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc tgaactgttc
     5521 tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat cgcgagtacg
     5581 ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc
     5641 actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc gtcatcatca
     5701 acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc tttcgcaact
     5761 tgagcaagca ccaccacaag gtacgctggc ctcgtaatta cttaattaca ccacaggcat
     5821 agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg ccgccaacgt
     5881 ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc gcgatgacgg
     5941 catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct aaatgcccgg
     6001 taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg cctgcggatt
     6061 ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg gcatgaatat
     6121 ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc agacctttat
     6181 cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac gtgacgaggt
     6241 tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc gccagaatgg
     6301 ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata agtgtaaacc
     6361 agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt cgtaccccag
     6421 tgcagcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt ctgcttcgac
     6481 agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct gcgacggtaa
     6541 acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact cctccgccac
     6601 cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac gaccaccacc
     6661 aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga gcgtgccatc
     6721 cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa gggtaaacgg
     6781 cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg
     6841 cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg catccattaa
     6901 cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat taattagcga
     6961 ctgttcggcg ctcttaatga tgtgattgcg gtcagtgatg taatgaccaa tgacgttttc
     7021 ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt cacgcaggtg
     7081 aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac cataaatatc
     7141 agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac tgaccagctg
     7201 gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac gggaaaactc
     7261 cgggccttcc ggcgcaatag taatacgctg aaccataatc gcttcctctt atcagatata
     7321 agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt cgtctgatga
     7381 cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc gcagcacctc
     7441 ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac tacgaaaaca
     7501 gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc tctggacggg
     7561 aaagaactct aattgctccg ccacctcgcc ctcctcagat aagattatta ccattattga
     7621 agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc tactacactc
     7681 caacgcatga acaaacacac cgaacatgat actcgcgaac atctcttggc gacgggcgag
     7741 caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact aaaaaccgct
     7801 gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt tggcgttgcc
     7861 atgcttgagc gtcattacgc cgcatatcac cagcgactgg ctgagttgct gcaatccggc
     7921 gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa ccagttttgc
     7981 caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg
     8041 tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcgc cctgctctcg
     8101 caggctctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga accgctacaa
     8161 caggcacaag tgctttacgc actatggctt ggcgcgaatc tgcaggccaa aatttcgcgc
     8221 agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc gacgcctgcc
     8281 gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc tgaaaaactg
     8341 tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg
     8401 acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc ggaatactat
     8461 cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cacaaatttc tgcccaggca
     8521 aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc atggaaaaaa
     8581 atcaccgctg gcgttcatgc tgaaaatggt catatggcct tgcagctgtg gcacaccgga
     8641 cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt
     8701 agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg cgttgaaaca
     8761 tccatgccgc gtgcgcttga actggaagag attccgggta tcgtcaatga tttccgtcag
     8821 gccattgcta acgcgcgtga agccgctttt gatctggtag agctccactc tgctcacggt
     8881 tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca gtacggcggc
     8941 agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat tgaagaatgg
     9001 ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa cacagataac
     9061 ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa acgcggcatt
     9121 gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac tgatgcgttc
     9181 cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc atacacagta
     9241 gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac
     9301 tggattgcga acccggatct ggtcgcccgc ttgcagctca aagctgagct taacccacag
     9361 cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc gacgttgtaa
     9421 tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt gaatctgtta
     9481 gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc
     9541 aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt accagcgaaa
     9601 acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg
     9661 tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact gcttatggtc
     9721 acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg
     9781 gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg
     9841 aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt cgcggtctgg
     9901 gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg catcgacaag
     9961 taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag atgtccgata
    10021 acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
    10081 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc
    10141 tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac
    10201 catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt gacccgaacg
    10261 atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt aaagttgtac
    10321 gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac aacgccaatt
    10381 ttgatcacag ctttatgatg gccgccgcag aacgcgcctc gctgaaacgt aacccgttcc
    10441 accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
    10501 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc
    10561 tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc
    10621 tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat
    10681 gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat attcacgggg
    10741 aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg tgttttcacc
    10801 ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc agtcgcaaac
    10861 ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg gctccgggaa
    10921 aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg gcgaagatac
    10981 ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga taaaagccct
    11041 gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg atgaacggcg
    11101 gcggcgcggc gaaacggtaa ttattgatga agtccacgcg gtagcgggca gtaaacgtgg
    11161 tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct cagcacagcg
    11221 aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc ttggtggcga
    11281 tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac gaattgtcgt
    11341 accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcaccggcg aagacagcca
    11401 tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg atgaagtgtt
    11461 gcgccatcgc tcgaccattg tctttactaa ttcacgcggg ctggcggaaa aactgacagc
    11521 acgattaaat gagctttacg ccgcacgctt acagcgttcc ccgtctatcg ccgttgatgc
    11581 ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtacaaagta gcgacgtttt
    11641 tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca ccgaacaggc
    11701 gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtcttgaac tggggattga
    11761 tatgggcgcg gtggatctgg tgattcaggt ggcaacgccg ctttctgttg ccagtgggtt
    11821 acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc tgtttttccc
    11881 ccgtacccgg cgtgatttag tcgattccgc agtcattgta gagtgtatgt tcgcaggcag
    11941 gctggaaaac ctgacaccac cgcataatcc tctcgacgtc cttgcgcagc aaaccgttgc
    12001 cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac gccgtaccgc
    12061 accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc tttccgggcg
    12121 ctatccctct ggcgattttt ctgcttttcg ccccaaactg gtctggaaca gggagaccgg
    12181 gatattgacc gcccgacctg gcgctcaatt gttggcggtt accagcggcg gcaccattcc
    12241 ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaaaaggccg gttcgcggcg
    12301 ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgaca ttatcactct
    12361 cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg tgactcctgc
    12421 tccgggtcgt tctgcccagc tccccttctg gcgtggtgaa ggtaacggac gtccggctga
    12481 attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg
    12541 gactattccc ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga
    12601 cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg
    12661 ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca
    12721 tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc
    12781 tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc
    12841 cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt
    12901 aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat
    12961 gccgggccgc acgccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca
    13021 gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga
    13081 atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg
    13141 tggcgaaatt caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt
    13201 attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg
    13261 ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc
    13321 gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc
    13381 tcctggtaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc
    13441 aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta
    13501 tctggaaaat cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg
    13561 tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac ggctaccaga
    13621 gtcattgcca gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg
    13681 ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg
    13741 tattgccatt gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct
    13801 gcaacaaaac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca
    13861 agccgccaga gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga
    13921 acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatctcc
    13981 aggcgtttat gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg
    14041 tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgcc
    14101 agaaatgctc gatgaattac tggcaaccgg agcggttatc tggtcgggac aaaaaaaagc
    14161 tgggtgaaga tgacggcctg gtggcactgc atctacagaa atatgccgca gaatcgttca
    14221 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    14281 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    14341 tcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    14401 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    14461 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    14521 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    14581 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    14641 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    14701 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    14761 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    14821 cgacacaagc cacgcaaaca cgccactata caccagtggc gctctccgcc aacgatccgg
    14881 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gtaccaacgc
    14941 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactctat ctggcgcaag
    15001 gcggcaaaaa aatgctggtc tggcaggaaa aggaggaatt actcgcccca gagattttcc
    15061 aggcgctgac caccgcactg cgtcgcgaac caaggctgcg ctttacgcta acagaagtga
    15121 atgatcttcc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    15181 gttcgccaca agggctggat tggggatagt tgacatcctc cacgccctga aagacgtggt
    15241 tccctgctac gttcaagctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    15301 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    15361 agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    15421 tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
    15481 tggcggaaca gcaagcacct gaccgccact ccagagctgc ttgtactcaa gctggcggcg
    15541 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
    15601 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
    15661 ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
    15721 cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
    15781 gttgttgctg aatttgacct tccggcttaa ctgtcgctga agtctcgcca gcgtcttctg
    15841 gtttttttga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
    15901 agccacgcca gcatccagcc cgatcattga tgctgaaggg tgaaccggag ttgatacttc
    15961 actttctgtc tgaatactga tgtaccacgt accgcaggac tggctgacag tgacattttt
    16021 cacaactccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt tcggcagaaa
    16081 aatacggctg ttttcctgat cgagcttaac gtcctgcggg tagcggaatg cagcattctg
    16141 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
    16201 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
    16261 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
    16321 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
    16381 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca cctggtctta actggaattt
    16441 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
    16501 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa
    16561 ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt acttttcaaa
    16621 tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
    16681 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
    16741 catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
    16801 aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc caatatccgt
    16861 catccgccag tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
    16921 tccccgccct gaaggacgag gttttacgac gcaccggata aaggactgac ggatgcccgt
    16981 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
    17041 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
    17101 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
    17161 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
    17221 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
    17281 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
    17341 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
    17401 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
    17461 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
    17521 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
    17581 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
    17641 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
    17701 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
    17761 ggcccatgct tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc
    17821 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
    17881 gaaaaaagcc gccagtaaaa gtatgaccaa aagcaaaacc gcttcttctg ttaaaaaatc
    17941 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
    18001 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
    18061 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
    18121 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
    18181 cagctcaccg cataccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
    18241 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
    18301 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
    18361 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
    18421 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
    18481 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
    18541 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
    18601 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
    18661 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
    18721 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
    18781 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
    18841 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
    18901 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
    18961 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
    19021 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
    19081 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
    19141 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
    19201 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
    19261 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
    19321 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
    19381 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
    19441 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
    19501 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
    19561 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
    19621 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
    19681 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
    19741 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
    19801 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
    19861 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
    19921 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
    19981 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
    20041 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
    20101 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
    20161 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
    20221 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
    20281 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
    20341 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
    20401 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
    20461 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
    20521 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
    20581 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
    20641 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
    20701 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
    20761 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
    20821 acacaaaaag tgatattacg catttttaca cactgtgatg aaaaaatctc ccgtcattta
    20881 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
    20941 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
    21001 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
    21061 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
    21121 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
    21181 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
    21241 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
    21301 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
    21361 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
    21421 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
    21481 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
    21541 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
    21601 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
    21661 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
    21721 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
    21781 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
    21841 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
    21901 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
    21961 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
    22021 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
    22081 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
    22141 ttccggctcc cgtagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
    22201 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
    22261 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
    22321 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
    22381 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
    22441 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
    22501 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
    22561 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
    22621 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
    22681 aagttcgaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
    22741 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
    22801 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
    22861 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
    22921 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacacgat gcagctcctg
    22981 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
    23041 tgaccacata gtctgcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
    23101 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
    23161 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
    23221 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
    23281 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
    23341 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
    23401 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
    23461 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
    23521 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
    23581 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
    23641 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
    23701 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
    23761 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
    23821 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
    23881 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcgc
    23941 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
    24001 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
    24061 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
    24121 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
    24181 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
    24241 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
    24301 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
    24361 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
    24421 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
    24481 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
    24541 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
    24601 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
    24661 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
    24721 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
    24781 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
    24841 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
    24901 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
    24961 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
    25021 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
    25081 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
    25141 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
    25201 ggactagcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
    25261 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
    25321 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
    25381 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
    25441 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
    25501 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
    25561 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
    25621 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
    25681 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
    25741 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
    25801 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
    25861 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
    25921 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
    25981 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
    26041 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
    26101 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
    26161 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
    26221 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
    26281 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
    26341 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
    26401 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
    26461 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
    26521 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
    26581 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
    26641 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
    26701 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
    26761 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
    26821 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
    26881 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    26941 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    27001 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct acatggaaaa
    27061 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    27121 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    27181 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    27241 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    27301 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    27361 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgag cctgcggtta tgaaacgact
    27421 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    27481 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    27541 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    27601 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcggaccgg
    27661 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    27721 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    27781 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    27841 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    27901 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    27961 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    28021 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    28081 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    28141 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    28201 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    28261 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    28321 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    28381 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    28441 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    28501 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    28561 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    28621 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgcccccc cacattcacc
    28681 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    28741 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    28801 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    28861 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    28921 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    28981 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgcaggca
    29041 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    29101 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    29161 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    29221 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    29281 aactcgcctt cagtaagttg cgtcgcgcca aggacctcgc cggtcttaac aatttgcaca
    29341 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    29401 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    29461 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    29521 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    29581 taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    29641 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    29701 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    29761 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    29821 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    29881 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    29941 gagaatcgct atggcaactt tgttacaact tcattttgct tttaatggcc catttggcga
    30001 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    30061 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    30121 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    30181 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    30241 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    30301 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    30361 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    30421 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    30481 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
    30541 ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct cgaatggcta
    30601 caaaagcaag aagccagcca tctccagcgt tatggcgtca aaaaagaaac cttgcacgat
    30661 cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt tttacgatta
    30721 gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg ccaggttacc
    30781 gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt acccagcgag
    30841 acgtttgatt tagcggtgat tgccacgggt cacgtctggc ctgatgaaga agaagcaacc
    30901 agaacgtatt ttcccagccc gtggtcaggc ctgatggaag caaaggtcga tgcgtgtaac
    30961 gtgggtatta tgggaacatc cttgagcgga ctggatgcgg caatggcagt ggctattcag
    31021 catggttcgt tcattgaaga tgataaacaa cacgtcgttt ttcaccgcga taacgcaagt
    31081 gaaaagctaa atattacgtt gatgtcgcgc acgggtattt tacccgaagc cgatttctat
    31141 tgccctattc cctacgagcc cttacacatc gtcaccgatc aggcattaaa tgctgagatt
    31201 caaaaaggtg aagagggcct tttggatcgg gtatttagat tgatagtaga ggaaatcaag
    31261 tttgctgatc cagactggag ccaacgcata gccttagaga gcctgaatgt cgattccttt
    31321 gctcaagcct ggtttgccga gcgcaaacaa cgcgacccat ttgactgggc agaaaaaaat
    31381 ctccaggaag tcgaacgcaa taaacgagaa aaacatactg ttccctggcg ttatgtcatt
    31441 ctgcgcctgc atgaagccgt acaggaaatt gttcctcatc tgaatgaaca cgaccataaa
    31501 cggttcagta aaggccttgc cagggttttc atcgataatt atgcggcaat cccttcagag
    31561 tctattcgtc gactactggc cttacgtgaa gcggggatca ttcatattct cgccctcggt
    31621 gaagactacg aaatggaaat taatgagtcg cgcaccgtcc tgaaaacgga agacaacagc
    31681 tactcgtttg acgtttttat tgatgcccgc ggacagcgtc cgcttaaagt gaaagatatt
    31741 cctttccctg ggctacgcga gcaattacag aaaacagggg atgaaatccc tgatgttggc
    31801 gaagattata cgttacagca acccgaagat attcgtggac gcgtagcgtt cggcgcgttg
    31861 ccctggttga tgcacgacca gcctttcgtt cagggactta cggcatgtgc agaaattggt
    31921 gaggcgatgg ctcgggcggt cgtaaagcct gcatcccgtg ctcgtcggcg tctttcgttt
    31981 gattaaaggt aaagctatca gcactcaaca aactgatgct gttcattaag acgatagcgg
    32041 actccggttc gaatattgtt ttcaccttca atggcgacgg cgaagcgaac tctttcgccg
    32101 tcatgatatg ccagtgcggc gcttccccct agcccgagaa taattgagtc gaccacgccc
    32161 gtgctcacta ccgttgaatt ttcaccgctg gcaatgatgt gtacattgtc gccgctgttg
    32221 gcaattctgg cgtttgcgcc aaagctggcg atttgcacca ggtcaccatt actggcgaca
    32281 tgacaccgtt cacccaatgt acaaacgcgt actcgcattc cggtattcgc aatccgacta
    32341 ctatctccag cgctgcttat ccgcgaagaa ttaccggtta cagcaacccg tgcgcgttca
    32401 ccggagctgc caatgtgact gttaaagccg acacttccaa tccgcacgct ataacccata
    32461 ctggcaattt gcgcggcata cccagcggtt gcgattcggg cgttatcctc acaacagccg
    32521 atgcgagcat gatcgtcgct gcgggcaaac tgactgcaaa atggtatctc tccggggtct
    32581 gtggccagtt tttgcataga ggaaacttcc tgatgcgcga agttttcatc cgccagccat
    32641 tttttccacg catactcaac cagactctcc gcccagtccg tatatccttc ctcaatcagt
    32701 gcatgacgaa tatcggcata actaccgccg cgtgggaaat ggcgcaggaa ccagcgatac
    32761 atcaccgctc cgatacgcca ttctcgcagg tctgctcgtg taatgatcat tcgttagtgc
    32821 cggtgatagc catcgaccat gcttttaaag gtttcgtgtg gcgtacgccc cgtggtgcaa
    32881 agataaagat gaccgaagat aaaaaagagg cttataaatg ccagagcaaa atgcgcctgc
    32941 aataaccagt atcttacgcc aggaaacaca tctcccacgg cttgcggata gagacacaac
    33001 agccccgtca atagtaacaa cggcagcaat ccatacatga caccaacata ggcgacctgc
    33061 tgtaagggat taaatttaga ctgtgttgtt gccgggaaag gatgttcttc cccctgcata
    33121 atgccaaaca aataaaatcg cgtttgtttt gccgctcgtt ccagccaccc ctgacgacga
    33181 atgcgatagt ggtgaccatt atccccaacg gcattgatca gcacaaagcc gagccagcat
    33241 gccagtaaca aaaatccgca aacttcatgc accgcaacca gacttttaac cgcagttgcg
    33301 cccaccatcg caaagtgatt tatcagccca cttgccagca acaatacaaa gagtaacgca
    33361 ttcgaccagt gccataatct gaccgctttg ctgtaaagat aaaccttctc accatgatcg
    33421 gtggctgatt tcttcacgcc acgagcacga agcaaggcgt gcagccccag caccaaccac
    33481 atcccaacca gcaatactcc ggcaatgatc aaccacaccg gccaaaattc gggagtgaac
    33541 tgtggcacat agttcgctaa ctggctctga aactgttcag catgttgcga cgggttcata
    33601 cattttcctt tttaatcaaa tgttgaccga accggcgata cagatgcggt tttccggcac
    33661 caggtagctg atattgatag tatttattat cctgcaacca cgcctgaatt tctgggctat
    33721 cttcgcgacc aaagatcaat gcgtgttccg gacaggcgct gacgcaaatg ggcggaaagc
    33781 cttttgccag tcgggactca gcgcagaaat cacatttatc tgccacttta gtgacagggt
    33841 taaggtagcg aacctggtac ggacaagcgc caatgcaata actgcagccg atgcactgcg
    33901 atttttctac ccgcacaatc ccctgctcat cacgccacga tgcccccgtc gggcatacgt
    33961 cgatgcacgg agcatcttcg cagtgctgac atgactggcg aaaaaagtga tactgcgttt
    34021 cgttgtcgtt atcggtgact ggaatgtgtg ctatcgataa acggcttccc tgagcgggga
    34081 cgtggttagt tttacggcat gcgcgggcgc aaatattaca gccattgcac cgtgactcgt
    34141 catgaatcat ggcataccga acttcctttt cttgcctcgt gttcgctaac agagaagacg
    34201 cagaaccagt aaaaaagatt actgttccca tccccagaac aaatttgcgt cgagtaaagg
    34261 acatctgatt atcccctaat gcagtggctg caactgtcca ccctatccat gacatctccc
    34321 tgaaatttca ggggttctga aactgctcta cctcgaacca ttcgcataag ccataagcct
    34381 tgccataggt cataaatcgc tcgctaaaca agtacaacac cgccgtcgat gactccacct
    34441 gccgtatatc ggcatattca ggtgcaacat ccatcgcggc gatcgcggct tcaatctgcg
    34501 cttcctggaa gtaatatggc gcttgcatca gcatggcgac tttgtaagga cgaggatacg
    34561 tctggcactc aaaacgaacg gcgtgtgcaa tggcgcgaca aatgtcctgc tccacaactt
    34621 gcaaagacat cgccgcgtag ttttcactca tggcctgcgt tgagtaatag taatcatcct
    34681 gacttcccgt catacaagcg atatccgcgt agtctccgtt ctgcttaatg ctttccagca
    34741 aaactgacag ttcctcttct gcaaccgaat agggtggcga gagaaacacg gtgcgcgcca
    34801 ccagctgccc agccgcagaa tgctggcgaa tgaactcagc caggatttca gcaggttctg
    34861 gttcggttac cgtatcaacg gggtgacgca tcccctgcaa ccattgccgc ttcgcctcgt
    34921 caacttcgct gactttcgct aacggcaatt catcctgatg gttcatctta cccattcctt
    34981 acgcaggcag tagattgtgt gctgccagat cggcggcaat atcttccagc cccagacgct
    35041 gcaatgtttc gcgggttgga caaccaagct gtgggtccca gcccatttct ttgtagaaca
    35101 tcgtgagcga agcatgcata tcgtcacgat ccattttgtc agtaccttca gtaaagaccg
    35161 ggatctgcgg atccttgtcg aaaacccagg aacagataag atcgtgttca ttacgcatat
    35221 ctttggtttg catcggtttt accgtgtagg cacgatgcaa cgtaaagata cgctctgcag
    35281 ctaaatctaa tttttcctga gtcatctctt cgccggtgat cgctttgaag aatttggctt
    35341 aaagcgccag atcgccccgg taattacggc ttttcagcgg cgaaacggtc attggccaga
    35401 cccagttgca cagagtgacg gcgttatgca aacagacccg caacagcgac cactttgcat
    35461 atttaatttt tgcgtcattg attggcgtgt agtttttggt ttcatcgtaa gcatcttcag
    35521 aaccaaaaag ttctttcgcc acttcacgtt gcagtttcaa tggcaagccg gaaccaataa
    35581 agttgatatg ggtatgcgtc atgcagtcac ggttgaacat acagttaaca atggaaccga
    35641 cctgcgctga cgcttcattg gcatggtgaa ccggatagcc aaacggcgac cagagtttat
    35701 ttttcgcgta gccccagtac tctttaccca aattccagcg ttctgcgatg gcatatgaac
    35761 catcagccag gtgactcagc tcacccacac gatgcgccag acggtagtaa aaatctttaa
    35821 tgaagttaac gtcacccgct tccagttgat cccagcgaat ttctgcatac tcttcagctg
    35881 gcagaacacg cttgaacaca cctttgctgt aacaataagt aaagtcgcga tgcaactgcc
    35941 cgtagttaca ccataggccg tagtcatcga acaaattcag accaaccagg ttaccaatca
    36001 cacgaccatc gtctttatct tcaaaatctt tcggcccgtt cgggaagatg gtggtatgga
    36061 caaagtttgc tacacaagtg ttaccacctg tgctgggcac gccaaactct ttcacccgag
    36121 gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccgctc atttttactg
    36181 tgtatttctc tgccgccggt cctaagtcaa aaacggattt ataggtacga aagccgaccg
    36241 tattctgatt gcctggcgga atttcacccg tttcaatcgg accaccttca gccgcgcccc
    36301 aaaacagccc tttacgtgct gtccagcgtg acttgggatc tgaatactct gcccacgatt
    36361 gtggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc atcatgtaat
    36421 cattgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc ccttcaaccg
    36481 caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg ccgctgtggt
    36541 tacggctatt caacatgcca gagagaggaa taaggttttc cccagcctga ccaatagccg
    36601 ccacacaggt ttccgggctg gtcaatcgac aaatttcttc cgtcgttgcg cgcgtccctt
    36661 ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc ttcagccata
    36721 ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat ttcatttgcg
    36781 ctgcaaaaaa gccacccata tgggcatcgc gactaaattt cctttggtaa aagtagaaag
    36841 tgaggtgata tttacgcgag aactacaggg ggcaccagat ccagttaatg ggccggtagc
    36901 aaagactaat ttattcgctt catcgaaagg tttcgtgcct agcggtactt cgtcatacat
    36961 aattttgtag ccgaagccca tgccaccgac aaaactttta aacttactgg aatcttcgag
    37021 ggtaatattt cctgtcgtga gattgactct taatatatta cctgtccaac cgttagccat
    37081 gattttttcc tttgcaagat tcacacagta atatctttcc actcgataat ttttaacgcc
    37141 cctgttgggc aggcgtttgc gcattcaccg cataacacac atttcgagga ttttttactt
    37201 tcggtattta cggtggccat catccacggg catgccgtag tacaggcgct acagccaata
    37261 caacgtttat gatcgacggt aatacagcct tctttctgct gccaggtaat cgcaccaatc
    37321 gggcagacgt tcatgcattg cggctctttg cattgacggc aggtgtccgc ggtatagttg
    37381 agatcgccat acaggccgcc gccagagcca accccgttgt cgccaaagaa ataattgcga
    37441 tggattttga tacgggagaa gaatgtccct actgagccat cgttgaagtt ggtacaagag
    37501 atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt
    37561 gtatttatca accctactgt gccgctatca atatcttcct gtttgcaacc caaaagtgac
    37621 agtaacgcag gggcaattgt tagaccagca aggccttttc ctgatatacg caagaattct
    37681 aatcgcgtta agccaatatc taatagtgga cgatcaaccg ggttcattta tttcgcatcc
    37741 tcttcgcaaa cacagtgctg acaattcagc agcctgatat taagcattca actaatacgc
    37801 tgctgactct gtccccaaca ccagatattt tccctaaccc gatagtgtat gagaaaagat
    37861 ttaacgccga taaactaatc ctttatggtt aggagaaaaa ataatgtgat tattctgagc
    37921 ccttaacatt gatcgttatc aattaaaatt acaaagcaag aaattataag cgaactgata
    37981 tttattatca tttgaaataa atttaactta agcgacaatc atttgtgacg ttcattgcag
    38041 aaaataaaaa taacattatc gctataaatt aaaatacata ccgaaattcg tataatttca
    38101 gcagttgtta tttagaatga ttaactggac cgcaactgaa gcaagaatat caggatgaag
    38161 cttaccgcct catcctgaat ggtcatttta ccagtgcgtt aatgacggtt tccatcgcct
    38221 gcttttccag ctcactgcgc tgaatgcgtt tattggctaa tgctgtacgt aatacgcctg
    38281 cgataacaat atgctttggt tcctgcccca gatcgcgcat ttccagcaca actttaccga
    38341 caactctgca catttctcgg tacagttcat cctcttttgt ccgatttccc attctttata
    38401 cctatttatc atgccaa
//
LOCUS       DAEBXD010000031        37629 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEBXD010000031 DAEBXD010000000
VERSION     DAEBXD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37629
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.031"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..176)
                     /locus_tag="KJL94_001638"
     CDS             complement(<1..176)
                     /locus_tag="KJL94_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBD4410032.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KJL94_001639"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KJL94_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD4410033.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KJL94_001640"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KJL94_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBD4410034.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KJL94_001641"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KJL94_001641"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBD4410035.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KJL94_001642"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KJL94_001642"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBD4410036.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KJL94_001643"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KJL94_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBD4410037.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="KJL94_001644"
     CDS             complement(5180..5422)
                     /locus_tag="KJL94_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBD4410038.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KJL94_001645"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KJL94_001645"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBD4410039.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KJL94_001646"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KJL94_001646"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBD4410040.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="KJL94_001647"
     CDS             complement(8339..9313)
                     /locus_tag="KJL94_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD4410041.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="KJL94_001648"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="KJL94_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBD4410042.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="KJL94_001649"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="KJL94_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBD4410043.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="KJL94_001650"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="KJL94_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBD4410044.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="KJL94_001651"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="KJL94_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBD4410045.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="KJL94_001652"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="KJL94_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBD4410046.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="KJL94_001653"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="KJL94_001653"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBD4410047.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="KJL94_001654"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="KJL94_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBD4410048.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="KJL94_001655"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="KJL94_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBD4410049.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KJL94_001656"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KJL94_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBD4410050.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="KJL94_001657"
     CDS             23180..23383
                     /locus_tag="KJL94_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBD4410051.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="KJL94_001658"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="KJL94_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBD4410052.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="KJL94_001659"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="KJL94_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBD4410053.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="KJL94_001660"
     CDS             26388..26660
                     /locus_tag="KJL94_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBD4410054.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KJL94_001661"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KJL94_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBD4410055.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="KJL94_001662"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="KJL94_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBD4410056.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="KJL94_001663"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="KJL94_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD4410057.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="KJL94_001664"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="KJL94_001664"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBD4410058.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="KJL94_001665"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="KJL94_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="KJL94_001666"
     CDS             complement(31907..32305)
                     /locus_tag="KJL94_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBD4410059.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="KJL94_001667"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="KJL94_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBD4410060.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33846..34274
                     /gene="rplM"
                     /locus_tag="KJL94_001668"
     CDS             33846..34274
                     /gene="rplM"
                     /locus_tag="KJL94_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBD4410061.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34290..34682
                     /gene="rpsI"
                     /locus_tag="KJL94_001669"
     CDS             34290..34682
                     /gene="rpsI"
                     /locus_tag="KJL94_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBD4410062.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35077..35715
                     /gene="sspA"
                     /locus_tag="KJL94_001670"
     CDS             35077..35715
                     /gene="sspA"
                     /locus_tag="KJL94_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBD4410063.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35721..36218
                     /gene="sspB"
                     /locus_tag="KJL94_001671"
     CDS             35721..36218
                     /gene="sspB"
                     /locus_tag="KJL94_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBD4410064.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVCFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="KJL94_001672"
     CDS             complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="KJL94_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD4410065.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9116 a         9416 c         9896 g         9201 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actagaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacagcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttccccaaa
    33721 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    33781 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    33841 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    33901 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    33961 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    34021 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    34081 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    34141 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    34201 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    34261 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    34321 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    34381 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    34441 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    34501 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    34561 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    34621 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    34681 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    34741 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ttttttgctg ccgtctgaaa
    34801 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    34861 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    34921 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    34981 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    35041 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    35101 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    35161 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    35221 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    35281 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    35341 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    35401 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    35461 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    35521 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    35581 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    35641 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    35701 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    35761 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    35821 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    35881 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg tgctttaacg
    35941 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    36001 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    36061 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    36121 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    36181 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    36241 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    36301 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    36361 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    36421 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    36481 ggaataagac tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa
    36541 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    36601 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    36661 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    36721 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    36781 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgacttcat cagcccgacg
    36841 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    36901 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    36961 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    37021 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    37081 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    37141 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    37201 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    37261 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    37321 agcaagctca ccatcgcgca actggctcct atgcgctcca tgtagcgggc ataaccgccc
    37381 accgccataa tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca
    37441 ttataaatgt cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac
    37501 cctaaccaga ctccgcacat catcaggaag acacctgcgg cagccagcac cacctgaggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaacatt
//
LOCUS       DAEBXD010000032        36833 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEBXD010000032 DAEBXD010000000
VERSION     DAEBXD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.032"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..247
                     /locus_tag="KJL94_001673"
     CDS             <1..247
                     /locus_tag="KJL94_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD4410066.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KJL94_001674"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KJL94_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBD4410067.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KJL94_001675"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KJL94_001675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD4410068.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="KJL94_001676"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="KJL94_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBD4410069.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="KJL94_001677"
     CDS             complement(3258..3542)
                     /locus_tag="KJL94_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410070.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KJL94_001678"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KJL94_001678"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBD4410071.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="KJL94_001679"
     CDS             complement(4443..5651)
                     /locus_tag="KJL94_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410072.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="KJL94_001680"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="KJL94_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBD4410073.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="KJL94_001681"
     CDS             complement(6446..8131)
                     /locus_tag="KJL94_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBD4410074.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="KJL94_001682"
     CDS             8401..8778
                     /locus_tag="KJL94_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBD4410075.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KJL94_001683"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KJL94_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBD4410076.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="KJL94_001684"
     CDS             9225..9512
                     /locus_tag="KJL94_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBD4410077.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="KJL94_001685"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="KJL94_001685"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBD4410078.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="KJL94_001686"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="KJL94_001686"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBD4410079.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="KJL94_001687"
     CDS             11269..11745
                     /locus_tag="KJL94_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBD4410080.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="KJL94_001688"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="KJL94_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBD4410081.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="KJL94_001689"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="KJL94_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBD4410082.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="KJL94_001690"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="KJL94_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBD4410083.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="KJL94_001691"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="KJL94_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBD4410084.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="KJL94_001692"
     CDS             16302..16790
                     /locus_tag="KJL94_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBD4410085.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="KJL94_001693"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="KJL94_001693"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBD4410086.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KJL94_001694"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KJL94_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBD4410087.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KJL94_001695"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KJL94_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBD4410088.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KJL94_001696"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KJL94_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBD4410089.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KJL94_001697"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KJL94_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4410090.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KJL94_001698"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KJL94_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBD4410091.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="KJL94_001699"
     CDS             complement(22242..22757)
                     /locus_tag="KJL94_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4410092.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="KJL94_001700"
     CDS             23406..25175
                     /locus_tag="KJL94_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD4410093.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="KJL94_001701"
     CDS             25386..25709
                     /locus_tag="KJL94_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBD4410094.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="KJL94_001702"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="KJL94_001702"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBD4410095.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="KJL94_001703"
     CDS             complement(26533..27546)
                     /locus_tag="KJL94_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD4410096.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="KJL94_001704"
     CDS             complement(27645..29075)
                     /locus_tag="KJL94_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBD4410097.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KJL94_001705"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KJL94_001705"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBD4410098.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KJL94_001706"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KJL94_001706"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBD4410099.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KJL94_001707"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KJL94_001707"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBD4410100.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KJL94_001708"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KJL94_001708"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBD4410101.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KJL94_001709"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KJL94_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBD4410102.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KJL94_001710"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KJL94_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBD4410103.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9213 a         9157 c         9442 g         9021 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaa
//
LOCUS       DAEBXD010000033        36520 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEBXD010000033 DAEBXD010000000
VERSION     DAEBXD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.033"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(156..1391)
                     /locus_tag="KJL94_001711"
     CDS             complement(156..1391)
                     /locus_tag="KJL94_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD4410104.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KJL94_001712"
     tRNA            complement(1538..1614)
                     /locus_tag="KJL94_001712"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KJL94_001713"
     tRNA            complement(1657..1743)
                     /locus_tag="KJL94_001713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KJL94_001714"
     tRNA            complement(1764..1839)
                     /locus_tag="KJL94_001714"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KJL94_001715"
     tRNA            complement(1898..1974)
                     /locus_tag="KJL94_001715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KJL94_001716"
     CDS             complement(2077..3462)
                     /locus_tag="KJL94_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4410105.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KJL94_001717"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KJL94_001717"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBD4410106.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KJL94_001718"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KJL94_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBD4410107.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KJL94_001719"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KJL94_001719"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBD4410108.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KJL94_001720"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KJL94_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBD4410109.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KJL94_001721"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KJL94_001721"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBD4410110.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KJL94_001722"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KJL94_001722"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBD4410111.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KJL94_001723"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KJL94_001723"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD4410112.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KJL94_001724"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KJL94_001724"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD4410113.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KJL94_001725"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KJL94_001725"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBD4410114.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KJL94_001726"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KJL94_001726"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBD4410115.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KJL94_001727"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KJL94_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBD4410116.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KJL94_001728"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KJL94_001728"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBD4410117.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KJL94_001729"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KJL94_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBD4410118.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KJL94_001730"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KJL94_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBD4410119.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KJL94_001731"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KJL94_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBD4410120.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KJL94_001732"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KJL94_001732"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBD4410121.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KJL94_001733"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KJL94_001733"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBD4410122.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCITWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KJL94_001734"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KJL94_001734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBD4410123.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KJL94_001735"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KJL94_001735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBD4410124.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KJL94_001736"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KJL94_001736"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBD4410125.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KJL94_001737"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KJL94_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBD4410126.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KJL94_001738"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KJL94_001738"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBD4410127.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KJL94_001739"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KJL94_001739"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD4410128.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KJL94_001740"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KJL94_001740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBD4410129.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KJL94_001741"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KJL94_001741"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBD4410130.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KJL94_001742"
     CDS             complement(31267..32913)
                     /locus_tag="KJL94_001742"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBD4410131.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KJL94_001743"
     CDS             complement(32916..32966)
                     /locus_tag="KJL94_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410132.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KJL94_001744"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KJL94_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBD4410133.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="KJL94_001745"
     CDS             33502..35022
                     /locus_tag="KJL94_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBD4410134.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KJL94_001746"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KJL94_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBD4410135.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="KJL94_001747"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="KJL94_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBD4410136.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="KJL94_001748"
     tRNA            complement(36439..36514)
                     /locus_tag="KJL94_001748"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8919 a         9996 c         9199 g         8406 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg tactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcatat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgtacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgca tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acattaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAEBXD010000034        35155 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEBXD010000034 DAEBXD010000000
VERSION     DAEBXD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35155
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.034"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            88..1116
                     /gene="murB"
                     /locus_tag="KJL94_001749"
     CDS             88..1116
                     /gene="murB"
                     /locus_tag="KJL94_001749"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBD4410137.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1113..2078
                     /gene="birA"
                     /locus_tag="KJL94_001750"
     CDS             1113..2078
                     /gene="birA"
                     /locus_tag="KJL94_001750"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBD4410138.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KJL94_001751"
                     /gene_synonym="panK"
     CDS             complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KJL94_001751"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBD4410139.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3419..3494
                     /locus_tag="KJL94_001752"
     tRNA            3419..3494
                     /locus_tag="KJL94_001752"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3452..3454,aa:Thr,seq:tgt)
     gene            3503..3587
                     /locus_tag="KJL94_001753"
     tRNA            3503..3587
                     /locus_tag="KJL94_001753"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Tyr,seq:gta)
     gene            3704..3778
                     /locus_tag="KJL94_001754"
     tRNA            3704..3778
                     /locus_tag="KJL94_001754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Gly,seq:tcc)
     gene            3785..3860
                     /locus_tag="KJL94_001755"
     tRNA            3785..3860
                     /locus_tag="KJL94_001755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Thr,seq:ggt)
     gene            3975..5159
                     /gene="tuf"
                     /locus_tag="KJL94_001756"
     CDS             3975..5159
                     /gene="tuf"
                     /locus_tag="KJL94_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4410140.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5389..5772
                     /gene="secE"
                     /locus_tag="KJL94_001757"
     CDS             5389..5772
                     /gene="secE"
                     /locus_tag="KJL94_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBD4410141.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5774..6319
                     /gene="nusG"
                     /locus_tag="KJL94_001758"
     CDS             5774..6319
                     /gene="nusG"
                     /locus_tag="KJL94_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBD4410142.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6478..6906
                     /gene="rplK"
                     /locus_tag="KJL94_001759"
     CDS             6478..6906
                     /gene="rplK"
                     /locus_tag="KJL94_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBD4410143.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6910..7614
                     /gene="rplA"
                     /locus_tag="KJL94_001760"
     CDS             6910..7614
                     /gene="rplA"
                     /locus_tag="KJL94_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBD4410144.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8027..8524
                     /gene="rplJ"
                     /locus_tag="KJL94_001761"
     CDS             8027..8524
                     /gene="rplJ"
                     /locus_tag="KJL94_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBD4410145.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8591..8956
                     /gene="rplL"
                     /locus_tag="KJL94_001762"
     CDS             8591..8956
                     /gene="rplL"
                     /locus_tag="KJL94_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBD4410146.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9276..13304
                     /gene="rpoB"
                     /locus_tag="KJL94_001763"
     CDS             9276..13304
                     /gene="rpoB"
                     /locus_tag="KJL94_001763"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBD4410147.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13381..17604
                     /gene="rpoC"
                     /locus_tag="KJL94_001764"
     CDS             13381..17604
                     /gene="rpoC"
                     /locus_tag="KJL94_001764"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBD4410148.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KJL94_001765"
     CDS             complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KJL94_001765"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBD4410149.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KJL94_001766"
     CDS             complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KJL94_001766"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBD4410150.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KJL94_001767"
     CDS             complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KJL94_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBD4410151.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KJL94_001768"
     CDS             complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KJL94_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBD4410152.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KJL94_001769"
     CDS             complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KJL94_001769"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBD4410153.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KJL94_001770"
     CDS             complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KJL94_001770"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBD4410154.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23291..23391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KJL94_001771"
     CDS             complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KJL94_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBD4410155.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24015..24788
                     /gene="nudC"
                     /locus_tag="KJL94_001772"
     CDS             24015..24788
                     /gene="nudC"
                     /locus_tag="KJL94_001772"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBD4410156.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24828..25892
                     /gene="hemE"
                     /locus_tag="KJL94_001773"
     CDS             24828..25892
                     /gene="hemE"
                     /locus_tag="KJL94_001773"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBD4410157.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26049..26720
                     /gene="nfi"
                     /locus_tag="KJL94_001774"
     CDS             26049..26720
                     /gene="nfi"
                     /locus_tag="KJL94_001774"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBD4410158.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26763..27353
                     /locus_tag="KJL94_001775"
     CDS             26763..27353
                     /locus_tag="KJL94_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBD4410159.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27540..27812
                     /gene="hupA"
                     /locus_tag="KJL94_001776"
     CDS             27540..27812
                     /gene="hupA"
                     /locus_tag="KJL94_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBD4410160.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27825..28520
                     /locus_tag="KJL94_001777"
     CDS             27825..28520
                     /locus_tag="KJL94_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBD4410161.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KJL94_001778"
     CDS             complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KJL94_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBD4410162.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29185..30561
                     /gene="zraS"
                     /locus_tag="KJL94_001779"
     CDS             29185..30561
                     /gene="zraS"
                     /locus_tag="KJL94_001779"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBD4410163.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATLLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30558..31883
                     /gene="zraR"
                     /locus_tag="KJL94_001780"
     CDS             30558..31883
                     /gene="zraR"
                     /locus_tag="KJL94_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBD4410164.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWSYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KJL94_001781"
     CDS             complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KJL94_001781"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBD4410165.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KJL94_001782"
     CDS             complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KJL94_001782"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBD4410166.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8384 a         8997 c         9570 g         8204 t
ORIGIN      
        1 ctacaaactc ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag
       61 cctcgttttt gcatcaggaa agcagctatg aaccactcct taaaaccctg gaacacattt
      121 ggcattgatc ataatgctca gcacattgta tgtgccgaag acgaacaaca attactcaat
      181 gcctggcagc atgcaaccgc agaaggacaa cccgttctta ttctgggtga aggaagtaat
      241 gtactttttc tggaagacta tcgcggcacg gtgatcatca accggatcaa aggtatcgaa
      301 attcatgatg aacctgatgc gtggtattta catgtaggag ccggagaaaa ctggcatcgc
      361 ctggtaaaat acactttgca ggaaggtatg cctggtctgg aaaatctggc attaattcct
      421 ggttgtgtcg gctcatcacc tatccagaat attggtgctt atggcgtaga attacagcga
      481 gtttgcgctt atgttgattg tgttgaactg gcgacaggca agcaagtgcg cttaactgcc
      541 aaagagtgcc gttttggcta tcgcgacagt atttttaaac atgaatacca ggatcgcttc
      601 gccattgtag ccgtaggtct gcgtctgcca aaagagtggc aacctgtact aacgtatggt
      661 gacttaactc gtctggatcc tacaacagta acgccacagc aagtatttaa tgcggtgtgt
      721 catatgcgca ccaccaaact ccctgatcca aaagtgaatg gcaatgccgg tagtttcttc
      781 aaaaaccctg ttgtatctgc cgaaacggct aaagcattac tggcgcaatt tccaacagca
      841 ccaaattacc cccaggcggg tggttcagta aaactggcag caggttggct tattgatcag
      901 tgccagctaa aagggatgca aatgggtggg gctgcggtgc accgtcaaca ggcgttagtt
      961 ctcattaatg aagacaatgc aaaaagcgaa gatgtggtgc aactggcaca ccatgtaaga
     1021 caaaaagtgg gtgaaaaatt taatgtctgg cttgagcctg aagtccgctt tattggtgca
     1081 tcaggtgaag tgagcgcagt ggagacaatt tcatgaagga taacaccgtg ccactgaaat
     1141 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaatgctgg
     1201 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
     1261 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta cttaatgctg
     1321 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt attgactcca
     1381 cgaatcagtt ccttcttgat cgtatcggag agcttaaatc gggcgatgcc tgtgttgcag
     1441 aataccagca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct tttggcgcaa
     1501 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
     1561 gtctggttat cggtatcgtg atggcggaag tattacgaaa gctgggagca gataaagttc
     1621 gtgtcaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attcttgtgg
     1681 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg
     1741 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
     1801 ggatcaatct tgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
     1861 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     1921 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac
     1981 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga
     2041 tgggcggtga aatatccctg cgtagtgcag aaaaataaga aaggggagta ttcgctcccc
     2101 tgcaaattat ttgcgtagtc tgacctcttc taccacatga ttagcacttt tcgtcaggat
     2161 taaactggcg cgctcacgag taggtagaat attttgcttt aagttcagcc agttgatctc
     2221 tttccacaat gtcatggcag tcttaatcgc ttcttcttta gttaatttcg cgtagttatg
     2281 aaaataggaa tccgggtctg taaaagcccc ttcgcggaat ttcagaaaac ggttgatata
     2341 ccacgtctga agtaagtctt ccggtgcatc aacatatatc gaaaaatcga caaaatcgga
     2401 aacaaataca tgatgtggat cgtgtggata atccatcccg ctctgtaaga catttaaccc
     2461 ttcaagaatt aaaatatcag gctgaacaac agttttatct ccatccggga tcacatcata
     2521 aataagatgc gagtaaacag gggctgtaac gtttggcacg ccggatttga gatcggaaac
     2581 aaacttcacc aggcgatgca tatcatacga ttccgggaag cctttcttct tcatcagacc
     2641 acgttctttc agaacctgat tagggtgaag gaagccatct gtagtgatca gttcaacacg
     2701 acgatgttcc ggccaacggc ttaatagcgc ctgcaataca cgggcggttg tacttttccc
     2761 caccgcgaca ctgccagcaa tactgataat gtaaggaatg cgttgcccgt tggtaccaag
     2821 aaactgttcc agaactgcct gacggcgcag attcgagctt atatagaagt tcagcaaacg
     2881 tgacaaaggt aaatagatct cggcaacttc ttctaacgag agatcttcat taataccttt
     2941 gagacgggcg atctcatctt ccgataacgt cataggtacg gaatcacgca gagctgccca
     3001 ctggttgcgg tcaaactgta ggtaaggcgt cattaacgtt tgctctttta tactcataag
     3061 catgtttctg gcggtcatag ctctatcggc aaagcgatat ttgccatggc gcgtcacagt
     3121 tctgttgaat gggttaatgg gcaggagggt aacaccagat ggggaggaat aataagaaaa
     3181 aatctctgcc aggaagctat cgttgaaaag caatgtgaca gatattccat ctgataaaaa
     3241 gaattatggt ttagcaggag cgcattgttg agcacaatga tgttgaaaaa gtgtgctaat
     3301 ctgccctccg ttcggctgtt tcttcatcgt gtagcataaa atgtgaccaa taaaacaaat
     3361 tatgcaattt tttagttgca tgaactcgca tgtctccata gaatgcgcgc tacttgatgc
     3421 cgacttagct cagtaggtag agcaactgac ttgtaatcag taggtcacca gttcgattcc
     3481 ggtagtcggc accatcaagt ccggtggggt tcccgagcgg ccaaagggag cagactgtaa
     3541 atctgccgtc acagacttcg aaggttcgaa tccttccccc accaccaatt tcggccacgc
     3601 gatggcgtag cccgagacga taagttcact taccggctcg aataaagaga gcttctctcg
     3661 atattcagtg cagaatgaaa atcaggtagc cgagttctag gatgcgggca tcgtataatg
     3721 gctattacct cagccttcca agctgatgat gcgggttcga ttcccgctgc ccgctccaag
     3781 atgtgctgat atagctcagt tggtagagcg cacccttggt aagggtgagg tcggcagttc
     3841 gaatctgcct atcagcacca cttcttttct cctccctgtt ttttccttct gtttattgca
     3901 ttcaacaagt cgggcatgtt gcctggttga tgtggtgata tcaccgattt atccgtgtct
     3961 tagagggaca atcgatgtct aaagaaaagt ttgaacgtac aaaaccgcac gttaacgtcg
     4021 gtactatcgg ccacgttgac catggtaaaa caacgctgac cgctgcaatc actaccgtac
     4081 tggctaaaac ctacggcggt gctgctcgcg cattcgacca gatcgataac gcgccggaag
     4141 aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc ccgacccgtc
     4201 actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg atcaccggtg
     4261 ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg atgccgcaga
     4321 ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc gtgttcctga
     4381 acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg gaagttcgtg
     4441 aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt ggttctgctc
     4501 tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg gctggcttcc
     4561 tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
     4621 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggca
     4681 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
     4741 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg gcgtgctggt gagaacgtag
     4801 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
     4861 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
     4921 aaggcggtcg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
     4981 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
     5041 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
     5101 caatccgtga aggcggccgt accgttggtg cgggcgttgt agcaaaagtt ctgagctaat
     5161 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
     5221 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
     5281 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
     5341 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
     5401 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
     5461 ttgctcctgg cggcgattgt cggcaactac ctttatcgcg acattatgct gccgctgcgt
     5521 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     5581 ggtaaagcta ccattgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     5641 ccgattcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     5701 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     5761 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     5821 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     5881 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     5941 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     6001 cgacgcgagc tggcacttgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     6061 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     6121 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     6181 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     6241 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     6301 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     6361 cgtgagattg gaatacaatt tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat
     6421 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     6481 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     6541 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     6601 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     6661 tacgctgacc gttctttcac tttcgttacc aagaccccgc cggcagcagt tctgctgaaa
     6721 aaagcggcag gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     6781 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     6841 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     6901 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgtgagaa agttgatgca
     6961 accaaacagt acgacatcaa cgaagctatc gcactgctga aagagctggc gactgctaaa
     7021 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     7081 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     7141 tttacccaag gtgcaaacgc tgaagctgct aaagctgcag gcgcagaact ggtaggtatg
     7201 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     7261 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg tggcctgatg
     7321 ccaaacccga aagtgggtac tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa
     7381 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     7441 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     7501 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     7561 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     7621 tacgtgggcg gtgattttgt ctacaatctt acccccacgt ataatgctta atgcagacgt
     7681 atatccgaga tattcgggtt gtggcaaggc ggcaactgag tgagtcgcca agagcatagc
     7741 taactatgtg actggtgcga atgaaggaag ccaacgccgc cacaagttga atagcgacgg
     7801 atagaaaaga tttgttcgtt ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga
     7861 gtttcgcaag aaacttaatc ccctgcgtag acggtgacag aacgctaaga ttattctttt
     7921 atattctggc ttgtttctgc tcaccgtaat taagacgctc tctccgtttg gaggagtgaa
     7981 gtgagttcca gagattttct ctggcaaaca tccaggagca aagctaatgg ctttaaatct
     8041 tcaagacaaa caagcgattg ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc
     8101 agtagttgcg gattcccgtg gcgtaactgt agataaaatg actgaactgc gtaaagcagg
     8161 tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga
     8221 aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt ccgaccctga ttgcatactc
     8281 tatggaacac ccgggcgctg ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa
     8341 atttgaggtc aaagccgctg cctttgaagg tgagctgatc ccggcgtctc agatcgaccg
     8401 cctggcaact ctgccgacct acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga
     8461 agcttcggct ggcaaactgg ttcgtactct ggctgctgta cgcgatgcga aagaagctgc
     8521 ttaatcgcag ttatcttttt aacgcattcg cttacgtata aacttattct gatattcagg
     8581 aacaatttaa atgtctatca ctaaagatca aatcattgaa gcagttgcag ctatgtctgt
     8641 aatggacgtt gtagaactga tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc
     8701 tgctgtagct gtagctgctg gcccggttga agctgctgaa gaaaaaactg aattcgacgt
     8761 aattctgaaa gctgctggcg ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac
     8821 tggcctgggt ctgaaagaag ctaaagactt ggtagaatct gcaccggctg ctctgaaaga
     8881 aggcgtgagc aaagacgacg cagaagcact gaaaaaagct ctggaagaag ctggcgctga
     8941 agttgaagtt aaataagcca acccttccgg ttgcagcctg agaaatcagg ctgatggctg
     9001 gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt
     9061 tgactactgc tgtgcctttc aatgcttgtt tctatcgacg acttaatata ctgcgacagg
     9121 acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg
     9181 gaaagtgttc cattttccgg tcaacaaaat agtgttgcac aaactgtccg ctcaatggac
     9241 agatgggtcg acttgtcagc gagctgagga accctatggt ttactcctat accgagaaaa
     9301 aacgtattcg taaggatttt ggtaaacgtc cacaagttct ggatgtacct tatctccttt
     9361 ctatccagct tgactcgttt cagaaattta tcgagcaaga tcctgaaggg cagtatggtc
     9421 tggaagctgc tttccgttcc gtattcccga ttcagagcta cagcggtaat tccgagctgc
     9481 aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg
     9541 gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag
     9601 cgccggaagg caccgtaaaa gacattaaag aacaagaagt ctacatgggc gaaattccgc
     9661 tcatgacaga caacggtacc tttgttatca acggtactga gcgtgttatc gtttcccagc
     9721 tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg taaaacccac tcttcgggta
     9781 aagtgctgta taacgcgcgc atcatccctt accgtggttc ctggctggac ttcgaattcg
     9841 atccgaagga caacctgttc gtacgtatcg accgtcgccg taaactgcct gcgaccatca
     9901 ttctgcgcgc cctgaactac accacagagc agatcctcga cctgttcttt gaaaaagtta
     9961 tctttgaaat ccgtgataac aagctgcaga tggaactggt gccggaacgc ctgcgtggtg
    10021 aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta
    10081 tcactgcgcg ccacattcgc cagctggaaa aagacgacgt caaactgatc gaagtcccgg
    10141 ttgagtacat cgcaggtaaa gtggttgcta aagactatat tgatgagtct accggcgagc
    10201 tgatctgcgc agcgaacatg gagctgagcc tggatctgct ggctaagctg agccagtctg
    10261 gtcacaagcg tatcgaaacg ctgttcacca acgatctgga tcacggccca tatatctctg
    10321 aaaccttacg tgtcgaccca actaacgacc gtctgagcgc actggtagaa atctaccgca
    10381 tgatgcgccc tggcgagccg ccgactcgtg aagcagctga aagcctgttc gagaacctgt
    10441 tcttctccga agaccgttat gacttgtctg cggttggtcg tatgaagttc aaccgttctc
    10501 tgctgcgcga agaaatcgaa ggttccggta ttctgagcaa agacgacatc attgatgtta
    10561 tgaaaaagct catcgatatc cgtaacggta aaggcgaagt cgatgatatc gaccacctcg
    10621 gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa ccagttccgc gttggcttgg
    10681 tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg cgatctggat accctgatgc
    10741 ctcaggatat gatcaacgcc aagccgattt ccgcagcagt gaaagagttc ttcggttcca
    10801 gccagctgtc tcagtttatg gaccagaaca acccgctgtc tgagattacg cacaaacgtc
    10861 gtatctccgc actcggccca ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag
    10921 acgtacaccc gactcactac ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca
    10981 tcggtctgat caactctctg tccgtgtacg cacagactaa cgaatacggc ttccttgaga
    11041 ctccgtatcg taaagtgacc gacggtgttg taactgacga aattcactac ctgtctgcta
    11101 tcgaagaagg caactacgtt atcgcccagg cgaactccaa cctggatgaa gaaggccact
    11161 tcgtagaaga cctggtaact tgccgtagca aaggcgaatc cagcttgttc agccgcgacc
    11221 aggttgacta catggacgta tccacccagc aggtggtatc cgtcggtgcg tccctgatcc
    11281 cgttcctgga acacgatgac gccaaccgtg cattgatggg tgcgaatatg caacgtcagg
    11341 ccgttccgac tctgcgcgct gataagccgc tggttggtac tggtatggaa cgtgctgttg
    11401 ccgttgactc cggtgtaact gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg
    11461 cttcccgtat cgttatcaaa gttaacgaag acgagatgta tccgggtgaa gcaggtatcg
    11521 acatctacaa cctgaccaaa tacacccgtt ctaaccagaa cacctgtatc aaccagatgc
    11581 cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt gctggcagac ggtccgtcca
    11641 ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg
    11701 gttacaactt cgaagactcc atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca
    11761 ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag
    11821 agatcaccgc tgacatcccg aacgtgggtg aagctgcgct ctccaaactg gatgaatccg
    11881 gtatcgttta cattggtgcg gaagtgaccg gtggcgacat tctggttggt aaggtaacgc
    11941 cgaaaggtga aactcagctg accccagaag aaaaactgct gcgtgcgatc ttcggtgaga
    12001 aagcctctga cgttaaagac tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta
    12061 tcgacgttca ggtctttact cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg
    12121 aagaaatgca gctcaaacag gcgaagaaag acctgtctga agaactgcag atcctcgaag
    12181 cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc
    12241 tcgacaaact gccgcgcgat cgctggctgg agctgggcct gacagacgaa gagaaacaaa
    12301 atcagctgga acagctggct gagcagtatg acgaactgaa acacgagttc gagaagaaac
    12361 tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct ggcaccgggc gtgctgaaga
    12421 ttgttaaggt atatctggcg gttaaacgcc gtatccagcc tggtgacaag atggcaggtc
    12481 gtcacggtaa caagggtgta atttctaaga tcaacccgat cgaagatatg ccttacgatg
    12541 aaaacggtac gccggtagac atcgtactga acccgctggg cgtaccgtct cgtatgaaca
    12601 tcggtcagat cctcgaaacc cacctgggta tggctgcgaa aggtatcggc gacaagatca
    12661 acgccatgct gaaacagcag caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt
    12721 acgatctggg cgctgacgtt cgtcagaaag ttgacctgag taccttcagc gatgaagaag
    12781 ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg
    12841 gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg cgacctgccg acttccggtc
    12901 agatccgcct gtacgatggt cgcactggtg aacagttcga gcgtccggta accgttggtt
    12961 acatgtacat gctgaaactg aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg
    13021 gttcttacag cctggttact cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc
    13081 gtttcgggga gatggaagtg tgggcgctgg aagcatacgg cgcagcatac accctgcagg
    13141 aaatgctcac tgttaagtct gatgacgtga acggtcgtac caagatgtat aaaaacatcg
    13201 tggacggcaa ccatcagatg gagccgggca tgccagaatc cttcaacgta ttgttgaaag
    13261 agattcgttc gctgggtatc aacatcgaac tggaagacga gtaattctcg ctcaaacagg
    13321 tcactgctgt cggggtaaaa cccggcagcg gattgtgcta actccgacgg gagcaaatcc
    13381 gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc
    13441 aaaattgctc tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag
    13501 ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt
    13561 atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac
    13621 cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag
    13681 cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct gaaatcgctg
    13741 ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac
    13801 tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg
    13861 actgaagagc agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg
    13921 ggtgcggaag caatccaggc tctgctgaag agcatggatc tggagcaaga gtgcgaacag
    13981 ctgcgtgaag agctgaacga aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt
    14041 atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac cagagtggat gatcctgacc
    14101 gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg tggtcgtttc
    14161 gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa
    14221 cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag
    14281 gaagcggtag acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac
    14341 aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta aacagggtcg tttccgtcag
    14401 aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg taatcaccgt aggtccatac
    14461 ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc tggagctgtt caaaccgttc
    14521 atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg
    14581 gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg
    14641 gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta
    14701 ctgatcgaag gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac
    14761 ttcgatggtg accagatggc tgttcacgta ccgctgacgc tggaagccca gctggaagcg
    14821 cgtgcgctga tgatgtctac caacaacatc ctgtccccgg cgaacggcga accaatcatc
    14881 gttccgtctc aggacgttgt actgggtctg tactacatga cccgtgactg tgttaacgcc
    14941 aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta tcgctctggt
    15001 ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac
    15061 ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg
    15121 atgattgtac cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca
    15181 atctccaaaa tgctgaacac ctgctaccgc attctcggtc tgaaaccgac cgttattttt
    15241 gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc gttctggtgc atctgttggc
    15301 atcgatgaca tggtcatccc ggagaagaaa cacgaaatca tctccgaggc agaagcagaa
    15361 gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga acgctacaac
    15421 aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac
    15481 ctgcaaactg aaaccgttat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac
    15541 agcatctaca tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt
    15601 gctggtatgc gtggtctgat ggcaaagccg gatggctcca tcatcgaaac gccaatcacc
    15661 gcgaacttcc gtgaaggtct gaacgtactc cagtacttca tctccaccca cggtgctcgt
    15721 aaaggtctgg cggataccgc actgaaaact gcgaactccg gttacctgac tcgtcgtctg
    15781 gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca tgaaggtatc
    15841 atgatgactc cggttatcga ggggggtgac gttaaagagc cgctgcgcga tcgcgtactg
    15901 ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatattct cgttccgcgc
    15961 aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggta
    16021 aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg tatgtgcgca ctgctacggt
    16081 cgtgacctgg cgcgtggcca catcatcaac aaaggtgaag caatcggtgt tatcgcggca
    16141 cagtccatcg gtgaaccggg tacacagctg accatgcgta cgttccacat cggtggtgcg
    16201 gcatctcgtg cggctgctga atccagcatc caggtgaaaa acaaaggtag catcaagctc
    16261 agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaacacc
    16321 gaactgaaac tgatcgacga attcggtcgt accaaagaaa gctacaaagt gccttacggt
    16381 gcggtactgg cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg
    16441 gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg
    16501 atcgacggcc agaccattac tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg
    16561 gttctggatt ccgcagaacg taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc
    16621 gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgcctgc gcagtacttc
    16681 ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc
    16741 ttggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc
    16801 gttgcggacc tgttcgaagc tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc
    16861 ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc gtctggttat caccccggta
    16921 gacggtagcg atccgtacga agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa
    16981 ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt
    17041 ctgcgtctgc gtggtgttca tgctgtgact cgttacatcg ttaacgaagt acaggacgta
    17101 taccgtctgc agggcgtaaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg
    17161 ctgcgtaaag ctaccatcgt taacgcgggc agctccgact tcctggaagg cgaacaggtt
    17221 gaatactctc gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca
    17281 acttactccc gcgatctgct gggtatcacc aaagcgtctc tggcaaccga gtccttcatc
    17341 tccgcggcat cgttccagga gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa
    17401 cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt
    17461 accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct
    17521 gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt
    17581 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
    17641 cgggtttttt tatgggtgtt attcagaggg caaattcttc acggaaaata ttaagaaagg
    17701 cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cggcactacc
    17761 ataaaaaaac acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa
    17821 taaatttacg ttcttacatt ccgtctcata gtctttgcga ggcgcgtccc agatagctgt
    17881 cccagtcttt ccataccggc tgcaaaccct gagcggttaa cacggcagca accgcttccg
    17941 gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg gtgattatcg gcatagccac
    18001 ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc cagcggaata acgcgatcgc
    18061 gaaaccacgg tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagtcgga
    18121 aggcgcagat ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc
    18181 cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg gctttgccag taatgctgtt
    18241 gcagccatag caaatgttcg gcaaccatat agcagtcaac gcgccagttg tcggaaaggc
    18301 caattagcgc gccgaggcct atcttatcaa tccccgcacg ccccagccga tccggcgttt
    18361 ccagccgcca gaagaagtcc tgttttttgc ctttcaaatg atggcgggta taagtcgcct
    18421 cgtgatatgt ctcctgataa accatcaccc catccagacc aagttgcttt aactcggcgt
    18481 attccgtctc cgccagcggt tgcacttcca tttgtagtga agagaactgt tcacgcaggg
    18541 cggggagatg acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta
    18601 atagcagatg ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt
    18661 catccagcgt tttgcgcttg atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt
    18721 tagcgcaaag attggaaaga taaagcggga cgtagaaact aactgtgttg ccaaatcgct
    18781 gacgggtcag acgctgcgcc tgttgggcca gttgttccag atagccactg gcggcaggcg
    18841 ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt
    18901 cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc
    18961 gattgctgaa ggttttcatg ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg
    19021 agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc agcaggccta cttctaccgc
    19081 cagacgaaat gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac
    19141 taacaccgcg tcggccccca tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc
    19201 agcatcgaca accaccggaa ctgtggcctg ctggataata atctccagca tggcacgggt
    19261 ttccagtccc tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac
    19321 ttcttccaga cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc
    19381 ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg
    19441 ggcgtcaggg tgaatctcca attttagcca gtttgtgcct aacgcttcac gtgccagatg
    19501 ggcggcgaaa atggcttctt ccgctgtttt cgccccggaa gtattcggca gcagggccac
    19561 acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt
    19621 catcgccagt gtcaccagct ggctgccgga agcgcggatc gcctccacca tcagttgtga
    19681 agaagcgaat ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg
    19741 taacatctca accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga
    19801 tatgttgcgt ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg
    19861 cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca
    19921 tcggttgatc gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg
    19981 cggcgcaacg ccaggctgcg ccactggctc gacttaccgt cgaacagtcg gagttctccc
    20041 gcaggcgtct ctataccgct taataactta atggcttcca gtgcctgcaa agtgcccatc
    20101 accccgacca ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta
    20161 tctggccaca ggcagcggta acacccctgc tcccagggcg gtgtcagtac catcaactga
    20221 ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca
    20281 ttaatctcct ggcgagtcgc catattgtcg gtacagtcga gcaccacatc ggcttgtgca
    20341 accgcatctt tcaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatgtcg
    20401 ggattcaact gtgtcagtcg ctgttggctg acctgcgatt tcgggctgtt gatatcttca
    20461 gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt
    20521 accagcgtcc cgacgccagc ccctgccagg tatagcgcag caggtgtacc cagcccgcca
    20581 agaccaataa tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg
    20641 tcgagcagaa tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc
    20701 aatttccagc aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac
    20761 gacggcgata ctgccgacac ccgttgctat caccgcaggc gcgcgtgcca gactgatacc
    20821 gccaatcgcc acggtgggat aatccgccag gcgctcaaca tgccgtgcca actgttccag
    20881 cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat
    20941 ataagagggg cgtgctgcca gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc
    21001 cagccgcagg cctgccgcgc ggatggcatt gagatcggtg gcttgcaaat cttcctgccc
    21061 caaatggacg ccatacgcct gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa
    21121 tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc
    21181 atcgcgccga tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc
    21241 gatccactgt acgctgtcca ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa
    21301 atcaggctga tacatcacgc ttcctcctta tgcaggtaga tttctccgcc tctggcacgg
    21361 aagttctccg acatatccgc cattcccact tcaatggttt gcgcggcggc gtaatcgcgt
    21421 acttcctggc tgattttcat cgagcagaat tttggcccac acatggagca aaaatgggcg
    21481 actttgccgg actcttgggg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg
    21541 agggccagat taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta
    21601 tcgcgaattt gcgcgcccgg atgccctttt gccaggtcag cagcgtgggc ggcaatctta
    21661 taagtgataa gcccctgctt aacatcttct ttattgggca gacccagatg ctcttttggc
    21721 gttacgtaac agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac
    21781 gtgaagtggt catagcccgg tgcaatatcg gtagttagcg gccccagagt gtaaaacggc
    21841 gcttcgtggc agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcaca
    21901 tggcctgggc cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttcg
    21961 cccagcgtat gcaattcggc aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg
    22021 cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg
    22081 cggaagtgtt gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc
    22141 gaaccgccgc gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc
    22201 agtacgcccg catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg
    22261 tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg
    22321 tagatcggca ctgtaccgat cggcaccgga ctgttacgca aaatccactc gcgggtttcg
    22381 tgaatatagc gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat
    22441 accagctttt ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg
    22501 ttaactttta ccaggaagtt acggccaata atcatcggct ccgattccgg atgattaatg
    22561 ttggccggga taatcgcacg tccggcagca acttcatcac ggacaaattc cgcagtgata
    22621 ttttccggca gatgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacctcgcta
    22681 cggatgcgct cgcggcccat attctcgcgg atggcgatga attccatgtc cggcgtgatg
    22741 atgccctggc gggcgtagtg cagttgggtg acacggcgtc ctgttttggc gcgttttggc
    22801 gttaataagc cgctaaaacg tagctcgtcg agaccatcat ctgccagccg cgttctggtg
    22861 taatcggaac tgcgcacggt aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg
    22921 cgtagttttg ccagcccttg ctgcacgtta atggcgatct gcggatcacc atacgggccg
    22981 gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact gcggctgttc tttgctaccg
    23041 ccaattagcg tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg
    23101 ccagtgatat aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa
    23161 tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat agctcattcc
    23221 aaaaagttaa ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag
    23281 gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg
    23341 cggatcccga ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagcccccac
    23401 aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga
    23461 cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca
    23521 gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg
    23581 cggtttccag gctggaatcg tagtaatcca taatctgttg ggtattggct tcgagttgcg
    23641 gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct
    23701 cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa
    23761 gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt
    23821 agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc
    23881 ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt
    23941 attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag
    24001 gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca
    24061 gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt
    24121 tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt
    24181 taatacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg
    24241 ttgggctgtt tcaactggcc ggacgtggcg tgcaactggc ggagttttac cgatcgcata
    24301 aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt
    24361 gtagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc
    24421 gtcgcgatga ttcgctcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag
    24481 tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga
    24541 tggaagagag cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt
    24601 ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg
    24661 acccgaaaga attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc
    24721 cgcccggcac cgtagcgcgc cgtctgatag aagatactgt ggcgatgtgt cgggcagagt
    24781 atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta
    24841 aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga
    24901 tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg
    24961 attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc
    25021 gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga
    25081 tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct
    25141 gcaaagccga cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga
    25201 tgaacgcggt gcgtaccatt cgtcacgaac tgaaaggcga agtgccgctg attggttttt
    25261 ccggcagccc gtggacgctg gcaacctaca tggtggaagg cggcagcagc aaagccttca
    25321 ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctctg ctcgataaac
    25381 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggcagtga
    25441 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
    25501 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag
    25561 tcacgctgtt taccaaaggc ggcggacagt ggctggaagc catggctgaa accggttgcg
    25621 atgcgctggg cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata
    25681 aagtcgcgtt gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg
    25741 aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc
    25801 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag
    25861 tgcatcgact gtctgaacag tatcaccgct aagagcctga tccatcaggc tattttattt
    25921 gccattttga ggccgggcag tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt
    25981 tccgcgctgt ccggcctcaa actgccttca acaattacgc ctgttgaacc aggctctaag
    26041 gagcgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga
    26101 tctgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt
    26161 ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg
    26221 agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt ccaggttttc
    26281 tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg
    26341 atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc
    26401 attttggctt attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta
    26461 aattcgaacc gctctccagc aaaccgggcg cgctggcccc actgatggat aaaggcgagc
    26521 agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc
    26581 atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc
    26641 tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct
    26701 atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt tgagaactca
    26761 acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg cagcatgtca
    26821 ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc tgccagcaaa
    26881 ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga
    26941 ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc
    27001 cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt
    27061 taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga
    27121 gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgcgaaatgg
    27181 gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa
    27241 tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg
    27301 cagacctgcg tgagtcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga
    27361 aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg
    27421 tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt
    27481 ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta
    27541 tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg
    27601 ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg
    27661 ctgtacaact ggttggtttc ggtaccttca aagtgaacca tcgcgctgag cgtactggcc
    27721 gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt
    27781 ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg
    27841 aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc
    27901 tgtttctcac tgcctgtagc cacaactctt cacttcctcc ctttaccgcc agtggatttg
    27961 ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc
    28021 ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg
    28081 gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg
    28141 cgcgttttga cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc
    28201 agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga
    28261 ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg
    28321 aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga
    28381 tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg
    28441 aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac
    28501 ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca
    28561 gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat
    28621 atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac
    28681 cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg
    28741 cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt
    28801 ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg
    28861 tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac
    28921 cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt
    28981 attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac
    29041 gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc
    29101 ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa
    29161 agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct
    29221 aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg
    29281 acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa
    29341 ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg
    29401 catcatgcgc agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg
    29461 tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga
    29521 aaacagcttt attcccctca ggaaatgcag cagttacatc cgggagatga agaagcgtgg
    29581 cggcggatcg atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg
    29641 atgtttgctg ctggaatgta ccggatgcgc catatgcagc agtatgccgc gacaccacaa
    29701 gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga
    29761 aacaccctga ttattctctt cgccctggcg acgctcttgc tggcaagcgt attgtcattc
    29821 ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa
    29881 gagaagctgg tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca
    29941 ctttcctcga ttaaaggact ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa
    30001 gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc
    30061 gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg
    30121 ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta
    30181 cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag
    30241 gtcctgttga atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc
    30301 gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga
    30361 attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc
    30421 accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag
    30481 gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg
    30541 cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca
    30601 ttagccactg cactattttg caggctttac tgcgcggctg gagctataac gtcgcgctgg
    30661 cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt
    30721 gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa
    30781 acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg
    30841 cactgaaaac tggggcgcag gattatctca tcaagccgct ggatttcgat aacctacagg
    30901 cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga
    30961 ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa
    31021 tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggcaccggta
    31081 aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa
    31141 cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa
    31201 aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg
    31261 gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc
    31321 gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg
    31381 tccggctgat tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc
    31441 gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac
    31501 ggcgggaaga tatccctctg ctggctggcc attttcttca gcgctttgcc gagcgtaatc
    31561 gcaaggcggt aaaaggcttt acgccacagg cgatggattt gctgatccac tacgactggc
    31621 cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg
    31681 aatatatttc cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc
    31741 aaagtcagga tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg
    31801 agaaaacggg cggcaacaaa accgaagccg cccgtcagtt agggatcacg cgcaaaacgc
    31861 tattggcaaa actgtcgcgt tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt
    31921 tccggcagaa gcaatcatcc cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg
    31981 cttctgccac ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg ctggttacca
    32041 cctgctcgtc atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt
    32101 ccagcggcag gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag
    32161 ccatcaccac gctgagagaa gcacgttcat cccactctga cgttttctcg tccagtttgc
    32221 cttcacaggc cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct
    32281 gggtttccgg atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt
    32341 cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt
    32401 tcacggttgg ccagatgata cgttccatgg tgcgctgatg aacttcatcg gttactaccg
    32461 gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc gttatcttta tcgcctacgc
    32521 gtttgtgatc ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa
    32581 agctcgcttc ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa
    32641 aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca
    32701 cgataacgcc tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct
    32761 cacgcagata caccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga
    32821 tcttatggcg ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac
    32881 ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca
    32941 ccagcggcgc ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca
    33001 acagcgccgg gatatcggtc acgccgatag caacgttttg cagcgcgggt tccagcgcag
    33061 tgcctgcatt acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc
    33121 aggccagcgc gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca
    33181 ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc
    33241 ggcaatcact tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc
    33301 agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga
    33361 acctttcact tccaggcctt catcggcagc tttaataccg gcgattttcg cggagtacac
    33421 gcggctcatc tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc
    33481 attggatttc acgaatttcg ccactttcca gcagaacagc gcatcacgca gttcctgttc
    33541 ggtcggctga cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg
    33601 atcctgaacc agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc
    33661 ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc
    33721 ttcgctggcg gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc
    33781 ggtttccgca tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc
    33841 ggttttgtac gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg
    33901 gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc
    33961 cgcatcggta tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt
    34021 agcaacggag gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt
    34081 ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg
    34141 accggcggct tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc
    34201 aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt
    34261 tgcaagcgtc agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct
    34321 gctcttcacc acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg
    34381 gccgccgata tcgatgttct caaccgcatc ttccagcgag cagccttcac gagcaacagt
    34441 ctgggcgaac ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc
    34501 cataatggca tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag
    34561 ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac
    34621 cggcagacct ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc
    34681 gcgtgcggaa agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag
    34741 agcgcggcgg actggacgac gttgttgcat ggtaaatccc ctgggtttga ctattacaga
    34801 gagcgttagc tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat
    34861 ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat
    34921 ggtgatcaca attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt
    34981 gtggataact ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc
    35041 agtcattttt ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgccttc
    35101 atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga agaga
//
LOCUS       DAEBXD010000035        34938 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEBXD010000035 DAEBXD010000000
VERSION     DAEBXD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34938
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.035"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..2261
                     /locus_tag="KJL94_001783"
     CDS             <1..2261
                     /locus_tag="KJL94_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBD4410167.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3347
                     /locus_tag="KJL94_001784"
                     /pseudo
     CDS             2258..3347
                     /locus_tag="KJL94_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(3435..4733)
                     /gene="exuT"
                     /locus_tag="KJL94_001785"
     CDS             complement(3435..4733)
                     /gene="exuT"
                     /locus_tag="KJL94_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBD4410168.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5217..6629
                     /gene="uxaC"
                     /locus_tag="KJL94_001786"
     CDS             5217..6629
                     /gene="uxaC"
                     /locus_tag="KJL94_001786"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBD4410169.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6644..8131
                     /gene="uxaA"
                     /locus_tag="KJL94_001787"
     CDS             6644..8131
                     /gene="uxaA"
                     /locus_tag="KJL94_001787"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBD4410170.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8214..8765
                     /locus_tag="KJL94_001788"
     CDS             8214..8765
                     /locus_tag="KJL94_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBD4410171.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8770..10014)
                     /gene="sstT"
                     /locus_tag="KJL94_001789"
     CDS             complement(8770..10014)
                     /gene="sstT"
                     /locus_tag="KJL94_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBD4410172.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10610..11575)
                     /gene="alx"
                     /locus_tag="KJL94_001790"
     CDS             complement(10610..11575)
                     /gene="alx"
                     /locus_tag="KJL94_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBD4410173.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11846..12844)
                     /locus_tag="KJL94_001791"
     CDS             complement(11846..12844)
                     /locus_tag="KJL94_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4410174.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12923..13615)
                     /locus_tag="KJL94_001792"
     CDS             complement(12923..13615)
                     /locus_tag="KJL94_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBD4410175.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13688..14191)
                     /locus_tag="KJL94_001793"
     CDS             complement(13688..14191)
                     /locus_tag="KJL94_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBD4410176.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14276..15412
                     /gene="rlmG"
                     /locus_tag="KJL94_001794"
     CDS             14276..15412
                     /gene="rlmG"
                     /locus_tag="KJL94_001794"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBD4410177.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15697..16011
                     /gene="higB"
                     /locus_tag="KJL94_001795"
     CDS             15697..16011
                     /gene="higB"
                     /locus_tag="KJL94_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBD4410178.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16008..16424
                     /gene="higA"
                     /locus_tag="KJL94_001796"
     CDS             16008..16424
                     /gene="higA"
                     /locus_tag="KJL94_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBD4410179.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16469..18487)
                     /gene="fadH"
                     /locus_tag="KJL94_001797"
     CDS             complement(16469..18487)
                     /gene="fadH"
                     /locus_tag="KJL94_001797"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBD4410180.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18713..21064)
                     /gene="ygjK"
                     /locus_tag="KJL94_001798"
     CDS             complement(18713..21064)
                     /gene="ygjK"
                     /locus_tag="KJL94_001798"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBD4410181.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21081..21500)
                     /locus_tag="KJL94_001799"
     CDS             complement(21081..21500)
                     /locus_tag="KJL94_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410182.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21478..21831)
                     /locus_tag="KJL94_001800"
     CDS             complement(21478..21831)
                     /locus_tag="KJL94_001800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410183.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21832..22152)
                     /locus_tag="KJL94_001801"
     CDS             complement(21832..22152)
                     /locus_tag="KJL94_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410184.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22286..23719)
                     /locus_tag="KJL94_001802"
     CDS             complement(22286..23719)
                     /locus_tag="KJL94_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4410185.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23782..24231)
                     /locus_tag="KJL94_001803"
     CDS             complement(23782..24231)
                     /locus_tag="KJL94_001803"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBD4410186.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24228..27305)
                     /gene="ebgA"
                     /locus_tag="KJL94_001804"
     CDS             complement(24228..27305)
                     /gene="ebgA"
                     /locus_tag="KJL94_001804"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBD4410187.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27488..28471)
                     /gene="ebgR"
                     /locus_tag="KJL94_001805"
     CDS             complement(27488..28471)
                     /gene="ebgR"
                     /locus_tag="KJL94_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBD4410188.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28689..29020
                     /locus_tag="KJL94_001806"
                     /pseudo
     CDS             28689..29020
                     /locus_tag="KJL94_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29062..30441)
                     /gene="ygjG"
                     /locus_tag="KJL94_001807"
     CDS             complement(29062..30441)
                     /gene="ygjG"
                     /locus_tag="KJL94_001807"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBD4410189.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30859..32379
                     /gene="aer"
                     /locus_tag="KJL94_001808"
                     /pseudo
     CDS             30859..32379
                     /gene="aer"
                     /locus_tag="KJL94_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32533..33156)
                     /gene="nfeR"
                     /locus_tag="KJL94_001809"
                     /gene_synonym="yqjI"
     CDS             complement(32533..33156)
                     /gene="nfeR"
                     /locus_tag="KJL94_001809"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBD4410190.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33433..34178
                     /gene="nfeF"
                     /locus_tag="KJL94_001810"
                     /pseudo
     CDS             33433..34178
                     /gene="nfeF"
                     /locus_tag="KJL94_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34232..34307)
                     /locus_tag="KJL94_001811"
     tRNA            complement(34232..34307)
                     /locus_tag="KJL94_001811"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34272..34274),aa:Ile,seq:cat)
     gene            34432..34938
                     /gene="mug"
                     /locus_tag="KJL94_001812"
                     /pseudo
     CDS             34432..34938
                     /gene="mug"
                     /locus_tag="KJL94_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8539 a         8974 c         9174 g         8251 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caagcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctaatctat cgtcagggag
     3061 ggaccaccac cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa
     3121 ccggtgggca aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga
     3181 aacgtgtgcg tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac
     3241 ccttgactct ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg
     3301 gcaaattgtc agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg
     3361 acaaacgctt ttttctatat aaacgacgaa gccgcctgat aaggcggctt cgtcgttata
     3421 acttacgtta aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca
     3481 atcgccggtt tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact
     3541 gccagaactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc
     3601 gcaaacagcg tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact
     3661 tcgttacgac cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg
     3721 gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc
     3781 atacccgggc caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc
     3841 aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg
     3901 aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg
     3961 taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgca
     4021 aggaaacgcg gcagtgcgat accccagaac tgacggttac gcaggatctg accaacggac
     4081 attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt
     4141 tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc
     4201 gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg
     4261 attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag
     4321 tagcctactg cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc
     4381 gccgggatca tcgctgcttc cgcggcaccg accgcaccac gagcaacagc taggccaccc
     4441 cagctacctg ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca
     4501 taaccgattt tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta
     4561 taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct
     4621 tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg
     4681 ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac
     4741 ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg
     4801 ttagttacga ccgggtaacc gaaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt
     4861 ttcgtgctaa ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg
     4921 gcaatttgca ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac
     4981 cttaaaaggt gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca
     5041 gcgaggccag tactggcgcg gcctgcagcg agatttacca ctttgagagt aattttttta
     5101 actacgttta ttgatctaac tcaagaaaat atcttcggac tctggaaatt ggtgtgataa
     5161 ctttgtcagc atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga
     5221 ctccgtttat gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg
     5281 actacgcaaa agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg
     5341 cggaagacta tcgttttaaa aacctgtatg acatctggtt gaagggcgat cactacaaat
     5401 ggcgcgctat gcgtaccaac ggtgtggctg agcgtctgtg tactggtgat gcgtctgacc
     5461 gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc
     5521 actggacgca cctcgaactg cgtcgtccgt tcggtatcac tggcaaattg ctttctccgt
     5581 caactgccga tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg
     5641 cgcgcggcat catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg
     5701 attctctgga gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc
     5761 cgagctggcg tccggacaaa gccttcaata tcgaacaggc gacctttaac gactacatgg
     5821 cgaagctggg cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct
     5881 tgaccaaacg tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg
     5941 acgtggtgat gtttgctgaa gcgaacgaag cggaactgga cagcattctg gcgcgccgtc
     6001 tggctggcga aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt
     6061 tccttggtgc cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc
     6121 gtaataacaa cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca
     6181 acgaccgtcc gatggcggaa gagttgtcta agctgctgag caagcagaac gaagaaaacc
     6241 tgctgccgaa aaccattctc tactgcctga acccgcgcga taacgaagtg ctgggcacca
     6301 tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt
     6361 ggtttaacga tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtc
     6421 tgctgagccg ctttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc
     6481 acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag
     6541 caccggcgga catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc
     6601 gtgactactt cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat
     6661 ccatgcgctg gataacgtcg cggtcgcttt agcagatttg gctgaaggca cagaagtcag
     6721 tgtcgataac cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt
     6781 aacggatatc gcaaaagggg ccaacgtcat caaatatggc ctgccgattg gttatgcatt
     6841 ggcggatatt gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga
     6901 tctggatcag tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcc
     6961 tgaagtgcag atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat
     7021 cctgccaacc gtgggctgtg tcaacggcat cgcgcggcaa atccagaacc gtttcctgaa
     7081 agagaccaac aacgccgaag gtaccgacgg cgtgttcctc ttcagccaca cctatggctg
     7141 ctcacaactg ggcgacgatc acatcaacac ccgcaccatg ctgcaaaaca tggtgcgcca
     7201 cccgaacgcg ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc
     7261 attccgtgaa acgttgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca
     7321 gcaggatgat gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg
     7381 caacgataaa cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg
     7441 ttctgacggt ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt
     7501 gattgctaac ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca
     7561 gttgctgatg gaccattgcc gcgacgaagc aacgtttgaa aaactggtca ccatggtcaa
     7621 cgacttcaaa cagtacttta ttgcccatga tcagccgatc tacgaaaacc catcaccggg
     7681 gaacaaagcg ggcggtatca ccacgctgga agacaaatca cttggctgta cccagaaagc
     7741 gggttccagt gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccagggct
     7801 gaacttgtta agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg
     7861 ctgccatatg gtgctgttca gtactggtcg cggcacgcca tacggtggat ttgtgccgac
     7921 ggtgaaaatc gccaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga
     7981 cgcgggtcag ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac
     8041 catcgttgag tttgccaacg gtaagcaaac ctgcaacgag cgtaacgact tccgcgaact
     8101 ggcgatcttc aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg
     8161 cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg ctaaggattt atcatgaccg
     8221 cgtattggct ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt
     8281 tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg
     8341 tacatttttt tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc
     8401 gtacattgat taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga
     8461 ctggcggaat tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta
     8521 cgattgtcag tacctgggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt
     8581 ggttttcaac gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacga
     8641 tgattgaggg tagttttctg cttatgaatg gcctgaatat cattcgtttc tggcggatgc
     8701 agaaaagggg aattgatccg tttaaagtag agaaaacccc tcccgccata gacgaaaggg
     8761 gttaaacaat taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc
     8821 tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa
     8881 tacgccgata ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa
     8941 catattacag gccagtggga tcagcagcaa agaacccccc gccacgccgg atgcgccaca
     9001 ggcacacaga gaagccacta cgctcaacag cagcgctgtg ggcagatcga ccggaatacc
     9061 cagcgtatta accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt
     9121 gatggtggct cccagcggaa tagaaactga ataggtatcg cgatccagat tcagcttttc
     9181 acacagcgcc atattcaccg gaatgttcgc tgcagagctg cgggtgaaga aggcatacac
     9241 accgctttcg cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca
     9301 caccagcaat gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag
     9361 cagttgcgcg tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag
     9421 cccaaaaata ccgaacggtg cgaagcgaat gaccagtttc accataaagg taacggcatt
     9481 cgacatatca ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaagccgag
     9541 gccgatcgcc cacaccagaa tcccgatgta gttacctttc agcagggcgt cgatggggtt
     9601 ggaaaccatg ctcattacca gtccgcgcat cacttcgaca atgcctgacg gcggcgaaat
     9661 atcacccgcg ctactggaca agtgcagggt agaagggaag gcaaagctga agactactgc
     9721 ggccagagct gcagagaagg tgcccagcag atagaggaac aaaataggac ggatattggt
     9781 tttctgcccg tgctggtggt tagcaataga tgccattacc agcatcaaca ccaggatggg
     9841 ggcaacagct ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc
     9901 gggttttgag atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac
     9961 caggctgcca tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg
    10021 atcctttcat tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg
    10081 taacaaagtg tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg
    10141 ctggatttta aagagaggtc atgttttgca acttaatggt tgcatttatt taacataaac
    10201 agagggaagg actggctact aaggctgatt agataacata tgggcaaaac tatttggaat
    10261 atgagaacgg ttgcgctcct ttaacgtgtt atgccatata aagtcactgg cgcagggagc
    10321 gcgcaggggg cggccaatcg ccgccgcccc ctgcaccccc aggctctggc gaacaaaatc
    10381 gccgctgcgc ggttccctca gcctatccct tgcggctacc gggtcgggcg caaggtaacg
    10441 tccctgtaag atgcgccctc gccccggcct ccagggaacg cttcggcgat tttgacgcca
    10501 ccaacacccg tgcggtcttt ttgtaaagag ctataagccg attacataat taatagctca
    10561 cctttgtctg gaatgagtta tctctaggaa tggcttaata tacagcgaat cattctcccc
    10621 gctgcttatc atgccgataa ttcacccagg cgttgataat aaacgtcatc accagaatgc
    10681 caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct
    10741 tgataccgat aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg
    10801 ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gattgcaaac aggtttgagg
    10861 tcagcacaat gaacgggtca gtggtcacgg cgaagatagc cggaatgcta tccacggcaa
    10921 aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca
    10981 atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac
    11041 cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg
    11101 ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact
    11161 gcgaaatcag ccagctgcct gtgaagatca tgatggtacg cagaacaatc gccccgagca
    11221 cgccatacac cagcacgcga cgttgtaatg ccgccggaac ggagaaatag ctgaacaaca
    11281 tcagccagac aaagacgtta tcgaccgcca gcgatttctc aatcagataa cccgtgagaa
    11341 aggccagggc ctgtggatcg gcaacggcgc gaccttcggt ttgcaccaga taccaccaga
    11401 aagcggcgtt aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt
    11461 tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata gccagcataa
    11521 tggcgacaac aacagcgaat ccgccccata gcaacggcgt gccgacagta ttcatagaag
    11581 ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat
    11641 agacctcgcc ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc
    11701 tttcgggttg cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc
    11761 aatgaagtta ctcccctttg caggtaacaa agtatgacag accattcagt cagtcaatgg
    11821 tctgttacat accttttaca ctgttttact ttggcaagta tagttttacg ctatctgccg
    11881 gaaaaatcac cccagtttgg cggcggatct cggtcagcag tttggctgtg atgcgactga
    11941 ccgccagccc tggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt
    12001 cgtagagcat ggtattaata tgctgcggct gggtgagatc ctgcatctgg ctgccacgcg
    12061 gcacaaagca gactttctgg cattcagaca gtttttcaat caccagcgat cccgcttcgc
    12121 cctgaatctc gcttcccagg acagaatcac tgacctttga gtgctgcaag gtgacgctga
    12181 aatcaccgta atccatcacc accacgccgt gggcgtcaac gccgcttgcc agcaaactcg
    12241 ccgttgcctg cacgcttttc ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa
    12301 agccgatatc cataattgaa ccgttagaga atgccggatt aaaggtgttg ggattctcac
    12361 cgtccaggta acgttgatac cgcgaggaat actggcaata gttgaaaaag actttacgca
    12421 gttttcctgc tttcggcagc gcctggcgca acaaatgaaa gttcggcagg caggcggttt
    12481 taaatgcctc aaacagcacc acctgatttt cgcgcgcaca ggcaatggcg gcatccactt
    12541 ccgccagatt cgacgccagc ggtttttcgc aaatcacatg aattttatgg ctaaggaaaa
    12601 gttgtgtctg ggaaaagtgc agggaattcg ggctggcaat atacaccgcg tcaatggcat
    12661 cgctttccgc catcgcttcc agcgacgtaa acagatgctc gacagaaaaa tcattggcga
    12721 agtgctgagc ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct
    12781 catgggcggc ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta
    12841 tcataacgcg tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat
    12901 cttcgacgtg cgcagatacg cattattctt tcgccggaca gccatgctca ctaaacggcc
    12961 caatcatgac cgatggcccg agatatttcg gctggcgatg gaggattttt gcgtcaatca
    13021 ccgcgctgac acgagcgagt ttctcccgca gttgagtata aaaaccgtgc ttcatattga
    13081 ggtctggcgc gttaaacccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc
    13141 gttggttatg aaattcttgc gagataatgg tgatatggtt ttccccaaac acctttttgg
    13201 cacgcactac cgaatccagc gttgagaatc cggcatagtc gcaaaagatc actttcgccg
    13261 gtacgccttt agcgatcaaa gcctgctgca tacctgatgc ttcatcgtaa tttttacgtc
    13321 cgttatcgcc actcaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg
    13381 tatcaatacg tcgggtaaag tagcgattac ccggcctcgc ccccagcaat aaacccacgt
    13441 tacgcgccgg gacggcgttg acatcgcccc aggtcagctg cttgctggcg ttgaccataa
    13501 cgcgatcggc gataaaaatg gtcgctcctg ccacaagtaa cagtaagcag gcaatcttta
    13561 gagtccggcg agaaaaacaa atacggagaa gaaggcgggc aaatgcgcgc agcatggtcg
    13621 ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaaagat aacgcctgac
    13681 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    13741 actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac gctttgttat
    13801 gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa aacggttccg
    13861 gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    13921 gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt
    13981 catacatcac tttattgatc ggcggggcat tacgcagaaa ctgatttttc agatcctgag
    14041 tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    14101 gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc agctgctcgg
    14161 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    14221 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    14281 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    14341 taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt tggacgacac
    14401 agaaatcccc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    14461 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    14521 gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    14581 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    14641 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    14701 caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc tcggcccgac
    14761 caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca atgaaccgcc
    14821 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    14881 taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct tctttatgca
    14941 acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta atggcgttat
    15001 tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc
    15061 gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttggatcg
    15121 cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt
    15181 gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat
    15241 gttccactac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg ttgccaaccg
    15301 ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca ctattgcgac
    15361 gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt aagattggga
    15421 gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac gttaccccta
    15481 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc tggaatcgcg
    15541 cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgtttttc
    15601 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt
    15661 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    15721 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    15781 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    15841 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    15901 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    15961 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    16021 ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc
    16081 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    16141 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    16201 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    16261 gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat
    16321 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    16381 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    16441 cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg
    16501 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag
    16561 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    16621 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    16681 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcagga
    16741 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc
    16801 gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc
    16861 caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct
    16921 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    16981 aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac
    17041 tcaatacctt cggatgatcg atcccgtcga taggcggaac acgcggcacg atcccactgg
    17101 cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca
    17161 gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg taaaactcct
    17221 ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgcgcatcaa
    17281 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    17341 cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc attttggttt
    17401 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga
    17461 ggcagccctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct gattgcgctt
    17521 ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata tcggcatcgc
    17581 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    17641 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    17701 gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg ataatggtcg
    17761 cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg ccgccgcctt
    17821 cgaccaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg acgcgttcgc
    17881 gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    17941 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcagagc
    18001 ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag cgggcgaaat
    18061 cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg
    18121 gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    18181 aaatttgcag ggcgattttg ccgccttcct gatgtacggc ttcggtaatg gtgcgatggt
    18241 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    18301 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    18361 acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc attgaaccca
    18421 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    18481 agctcataaa attgtccagt atgtaaaata attgttatgt agtcggatga gttctaattt
    18541 agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    18601 atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc ttgggggatt
    18661 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct
    18721 tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg
    18781 cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    18841 atcctttggc gtgcctgaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt
    18901 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt
    18961 agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga acaaaggtgt
    19021 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg
    19081 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    19141 ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag
    19201 tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    19261 gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc
    19321 tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    19381 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    19441 cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacggc
    19501 ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct
    19561 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    19621 ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc gcgccatatt
    19681 ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    19741 gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat acttccatca
    19801 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    19861 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    19921 gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    19981 tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    20041 gattgccgga gaaccagcgt ccggtcaccg acggcgtgac ggtgttataa ttcaccgcac
    20101 cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    20161 gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct
    20221 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    20281 tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt
    20341 tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt
    20401 tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc
    20461 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt
    20521 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    20581 gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt tccagtagtg
    20641 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt gctgtcagtt
    20701 tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact ttcttgccgt
    20761 cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat tcttccgtca
    20821 gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac agatgaccat
    20881 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    20941 tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    21001 catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gttttgattt
    21061 tcatgagatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    21121 ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    21181 ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    21241 tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccttt taacgcttca
    21301 tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc gtcggcacgt
    21361 ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt gagatagagt
    21421 tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc ggcaaggtca
    21481 tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt agcgatcgta
    21541 cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac
    21601 gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa tgtcgcgggt
    21661 attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca ggtcatccag
    21721 cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac gataaccgcc
    21781 atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca cctaggcatt
    21841 accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga accaggagcc
    21901 gtacttcatc tccaggccac cttcaatata ggtgccgtcg ctgtatttat gtttattctc
    21961 accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa atgaaggaga
    22021 agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc
    22081 tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc atggtgcagt
    22141 gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt tatcgttgta
    22201 attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa gagggaaaat
    22261 gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc gcttcaatat
    22321 ggcgctcttc agccgttaat ccctttatat atttgctgta tttccaccac gcaaagccga
    22381 ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc gcgccagtcg
    22441 ggaatgtcga agcgacaaac cctacggcaa atatcgcaat cagcattgaa acaacgataa
    22501 taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat ttggcgcgta
    22561 aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg gcggtcatat
    22621 taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg ataatcatca
    22681 gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg ttttcgttaa
    22741 gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa attttcaccg
    22801 gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc agaccgacaa
    22861 agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg aatacctgca
    22921 ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc agcaccgagg
    22981 agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta acgaacgatt
    23041 tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca cctgcggcca
    23101 taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc atcgcgtcaa
    23161 cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc agtaaaatgt
    23221 aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc cccagcattt
    23281 tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg ataacggtgg
    23341 tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca taagcaataa
    23401 cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg taggcagtaa
    23461 taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg gcttctgagt
    23521 ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa taaaaaatcg
    23581 tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta ttgataacgt
    23641 tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg ccgattgtat
    23701 tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg tggcggcaaa
    23761 ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga tggtgacttt
    23821 gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat ggatatcgca
    23881 aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta aatattcacg
    23941 gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg catattcaat
    24001 tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc cggtaaatag
    24061 cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga tggagtgggc
    24121 gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt gccacttctt
    24181 gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca ttgcttattc
    24241 tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc caggctttgc
    24301 gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa gtcacggaac
    24361 cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag gccaagtagc
    24421 tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca gtgctgggca
    24481 gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg ctgcggaacc
    24541 accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg ctgacggttg
    24601 ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct gcgccagata
    24661 tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg accgtaatac
    24721 gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg aatgatgtgc
    24781 ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc ggcagcgatg
    24841 cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat aactgtgcgg
    24901 ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag atgttcctgc
    24961 atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg gttgtcgatc
    25021 atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc atttacctgg
    25081 caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta gccgcgaacg
    25141 gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg tgcgaaaggc
    25201 actggctgcg cggtgttttc cttcagcggg aactgataag aggcgataga atgcccggct
    25261 tcgctgtagc gggtgcggga atctttggtc accgtaatgt tgaggaacgc ttcgcgggcg
    25321 tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac gtcgcgcagt
    25381 ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg cagggtgtag
    25441 tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg agtcagcgcg
    25501 tggattttta ccggcgcgat aacctgtttg tactctttca ggcccgatcc cggcgtctga
    25561 tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc gccgccgaat
    25621 ttataccaga cattgccgtg gtcgtcctgt gcctggatcc cgtggtcgca ccactcccag
    25681 acataatgac cctgaatgca atcgtgctta tagaagacgt tctggtactc cgtcagcccg
    25741 cccggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt cggatgcggg
    25801 tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat aatatcgacc
    25861 acttcagcat cgcgatcttc ttcgtaatga accagtcgcg tgtcatccag cgctttcgcc
    25921 gcatggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag cgaccagatg
    25981 atgatcgacg gatggttttt ctgtgcgtgg atatggcgaa caatgcgctc gacgtagact
    26041 ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc
    26101 gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag ttcgtaaaaa
    26161 cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg cttcatcaac
    26221 tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg atcgttgtcg
    26281 tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag accgtcgcgc
    26341 actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt gccgttggcg
    26401 tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca ttgctgcggc
    26461 tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa atgatcaatg
    26521 gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt cgtgacgaca
    26581 ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc gcaataggct
    26641 tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt tccgaccaga
    26701 taaacatcgc ggaagatccc cgccgaccac cacatatcct ggtcttccac gtaggtagag
    26761 tccgcccact gcatcacgcg cacacacaac aggttgtcgc cggttttaac catcgcgctg
    26821 atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac ccacatactg accgttaacg
    26881 tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt accctgccag
    26941 ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct ggggacaaac
    27001 ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc
    27061 atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc agaggtgaag
    27121 gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgact gcttaagggc
    27181 agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga atcatatgaa
    27241 aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga
    27301 ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt agtttgtgta
    27361 attgctgggg ggttagtaaa atttttacta aaaagcagca ggtaaatgca tttactttaa
    27421 ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa tgattgccgg acagtgtgta
    27481 aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag actagcagcg
    27541 gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga cttcccatca
    27601 tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg gtggggatat
    27661 cgttaacgct gataagcgaa atatcttgtg ggatgttcag gccacgttca tgaattgccc
    27721 gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga tagtcttccc
    27781 gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg ccgcgccaga
    27841 tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg acctcacgaa
    27901 tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta acgccctggt
    27961 tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc accgcatcgt
    28021 aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac gcgctggcag
    28081 cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg tttttaatgt
    28141 ctggtaatcc gttgtgttca taacagttgg tcagttcgat cgccagcttt tcgcactgag
    28201 tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc tcctgctggt
    28261 agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt ttacgggcgc
    28321 tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc gtctcttctt
    28381 tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg gatacgccag
    28441 cttcgattgc gatgtctttt aatgttgcca tgtttacctt ttttactttc cctgatgtgc
    28501 gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata aagcgcgttt
    28561 ctttgacgct gtaacggtaa cacagcggct taggaatgtg atcacgcctg cattctccat
    28621 ttttagccta caggattgag aaaaactttt ctatagttta gtaaaatttt atccgaaggg
    28681 aggaagcaat ggaaaccgtg gcttacgctg attttgcacg tctggaaatg cgcgtcggaa
    28741 agattgtgga agtgaaacgc catgaaaacg ccgacaagct gtacatcgta caggttgatg
    28801 tggggaaaaa acactgcaaa ccgtgaccag cctggtgccg tactacagcg aagaggagct
    28861 gatggggaaa acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc gtggtgaaac
    28921 gtcggaatgc atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg tgttgttaac
    28981 gccggagcgg atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga tggcgacgtc
    29041 gtatcgccat ccgatttgat attacgcttc ttcgacactt actcgcatgg ccgccagcgc
    29101 cttacgcgcc gctttgatca ccagttcaca ctgttcaatg gtcagtgtca gtggcggttc
    29161 aatgcggatc gttttggcgt tattgagcgt tccggccacc agtacgcgct ggcggaacat
    29221 ctcgctggca aagttatagc cgatttcgtt atcaacaaac tcaatcgcca tcaacatccc
    29281 tttaccacgc gcttcctgta ccagatcggg atattcccgc gccagttgac ggaaaccgtc
    29341 cagcaacata tcgccttttt gctcagcctg agccggtaag ttctgctcca gcaacacatt
    29401 gatggtcgcc agcgccgccg cacaggccag cgggttgccg ccaaaggtgg tggtatgcag
    29461 gaatgggttg tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg cgccaatcgg
    29521 catcacgccg ccgccgaggg cttttgccag acacagaatg tccggctgaa cgttctcatg
    29581 ttcgcaggca aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat cgaggatcat
    29641 cagtgcgccg aactcatcgc acagcttacg cacggcggtg agatagcccg gcggcggcag
    29701 aattacgcca ccttcgccct gaatcggttc gaggatcacc gcagccacat catcaccggt
    29761 ttttttgcac tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg gcacatgacg
    29821 gaaccctggc agcagtggca taaacggttt gcggaaggtc gatttcgccg tggccgacag
    29881 cgcgccaagt gatttaccgt ggaacgcgcc gctggtggca ataaaagtaa atttgccgcg
    29941 cggcgactga taagccttcg ccagcttcag cgccgcttca acggactcgg tgccgctatt
    30001 acagaagaag ctgtatttca gtttaccggg cgttagcgca gcaagggttt tcgccagcat
    30061 agcccgtaac ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa gttgattctg
    30121 tacggcggaa accacaactg gattacggtg ccccacgttg aaaattccaa aacctcccag
    30181 gcagtcgata aactcctgtc cctgggtgtc gacaagcgta tttaaacctc ccgcttgcca
    30241 ctctacggct ccgtaatccc cgccggcggt aacagatttg cgatactcta aaaaccccgg
    30301 attgacatgc tctttgaagt attcaatcac ctctcggtta agtgctttca tctcctcatg
    30361 atccagcgtt cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta aagccgatgc
    30421 gctcgaaggt aacctgttca aaatatgctc cggaggctcg cgtatcacat gataccgatt
    30481 taagtattgc agggattgcg ccactccggc tgcgatcgcg aaaatcggga taaacacaaa
    30541 tgcggttatc atttgtgcaa tatttaatgc tatccgttaa catttgttgt tattttgcac
    30601 ggtttttgtc gggtatggat ttgatgattg ctgcgcaaat gcacgttttc agggcaataa
    30661 ttgcctgatt tttgggcaag ataattaacg ttttcgtata gtaacagagt gaaagtattt
    30721 taaatcaaag aattagattt gtgctttaat cgcaaattgc gatctaaatc aaattaatcg
    30781 gttaaagata accgcagcgg ggccgacata aactctgaca agaagttaac aaccatataa
    30841 cctgcacagg acgcgaacat gtcttctcat ccgtatgtca cccagcaaaa taccccgctg
    30901 gcggacgata ccactctgat gtccactacc gatctgcaaa gctatatcac tcatgctaat
    30961 gacacttttg tgcaggtgag cggctttacc ttgcaagagt tacaagggca gccgcacaac
    31021 atggtgcgtc acccggatat gccaaaagcg gcgtttgcgg atatgtggtt caccctgaaa
    31081 aaaggggagc cctggagcgg catcgtgaaa aatcgccgca aaaatggcga ccattattgg
    31141 gtgcgggcca atgcggtacc gatggtgcgc gagggaaaaa tcagtggcta tatgtcgatt
    31201 cgtacccggg cgacggatga agagattgcg gcggtggagc cgctgtacaa agcgttgaac
    31261 tccggacgta ccagtaagcg tattcataaa ggcctggtgg tgcgtaaagg ctggctgggt
    31321 aaactgcctt cattaccgct tcgctggcgg acgcgtggag tgatgaccct gatgtttatc
    31381 ttgctggcgg ccatgctttg gtttgttgct gccccggtgg tgacgtattt cctctgtgtg
    31441 ttagtggtat tgttggcaag cgcttgtttt gaatggcaga ttgtgcgccc gatagaaaat
    31501 gtcgcccgtc aggcactgaa ggtggcgacc ggagagcgta atagtgttga gcacctgaat
    31561 cgcagcgatg agctggggct gacattacgc gcggtagggc agcttggcct gatgtgccgt
    31621 tggctaatta acgatgtctc aagccaggtg tccagtgtca gaaatggcag tgagacgctg
    31681 gcgaaaggca ccgatgaact gaacgaacat acccagcaga cagttgataa cgttcagcaa
    31741 acggtggcga ccatgaacca aatggcggcg tcggtgaaac agaactctgc cacggcgtcg
    31801 gctgccgata aactttctat caccgccagt aatgcggcag tgcagggtgg ggaggcgatg
    31861 accacggtga tcaagacaat ggacgatatc gccgacagta cctagcgcat tggcaccatt
    31921 acttcgctga ttaacgatat tgcgtttcag accaatattc tggccctgaa tgcggcggtg
    31981 gaagcggcgc gtgccggcga acagggcaaa ggttttgcag tggtggcagg ggaagtgcgt
    32041 catttagcca gccgcagcgc taatgctgcc aacgatattc gcaagctgat tgatgccagt
    32101 gctgataagg tgcaatccgg ttcgcagcag gtacacgccg ccggacggac gatggaagat
    32161 attgtggcac aggtgaaaaa cgtcacccag ttgatcgccc agattagcca ttcaacgctg
    32221 gaacaggccg atgggctttc cagcctgacc cgtgcagtgg atgagcttaa cctgatcacc
    32281 cagaaaaatg ccgagctggt ggaagagagt gcgcaggtgt cggcgatggt gaaacaccgc
    32341 gccagccgac tggaagacgc ggtgacggtg ctgcattaat cggtatgccg gatccggcgg
    32401 ttggagcaca atgcctgatg cgatgctggc gcatcttatc aggcctgcgg ggtgtgtagc
    32461 gggtgtaggc cggataaggc gcgtcaagcg tcgcatccgg caattgcacc gcgccactgg
    32521 cggatgcggc gattaatcca gttgcgtaat atcaaaagcg gcgcggtcga tcaccgcaat
    32581 gatctttttt atttgtgcat cactgatatc gctctggttg acgcgtaaat ccagcaccgc
    32641 tttaaaatta tccagcgccc gcttcatttg cgggttctgg cgcagcgccg cgccaacgca
    32701 acgcgctttg atgcgttctt caatcatctc cacttgttcg cggttttctt ccagccactg
    32761 cgcaccttgt tcggtcagcg caatctgctt cttaccacct tcctcttcgc ggatggtaat
    32821 cagcgactgc tcctgcagaa aatccagcgt cgggtagatg acgcccgggc ttggggtgta
    32881 attcccctgg gttagattct caatcgcttt aatcaattcg taaccgtggc tgtcatcgcg
    32941 cgagagaata tccagaatca ccagacgcaa ttcaccgtga ccaaagaaac gttgccgacg
    33001 accgccgccg cgaccgtggc gaccaccgca acattgaccg tgttcatgct ggtgaccgtg
    33061 tccgcagtgc tcgtgttctg acttctcacc tttgcagcag ccctcatgcc gtggctggcc
    33121 ttcatgttta caacaccctt cgtgatgatg gctcatatca gcctcctttt gcttgattag
    33181 atatatctaa tttatatctg atgtgagcat tactgcaagt acaaaaatga tttttaaacg
    33241 tggaagcacc tcacatctag gccggataag acgttcacgc cgcatccggc agcattgcta
    33301 tggcactgaa tgccttcaca tacttcagac atatcagttc tacaaatcgc ttgcatttat
    33361 catgattaac aatcattatc atttgcgaat tttatttaga tatatctgat taccatcacg
    33421 aaggcgataa caatgaataa caccccccgc tacccgcagc gcgttcgcaa tgatctgcgc
    33481 ttccgtgaac tgactgtgct acgcgttgag cgcatcagcg ccggttttca gcgcattgtc
    33541 cttggcggcg aggcgttgga cggttttaca tcgcgcggct ttgacgatca cagcaaactc
    33601 ttctttcctc aacctgacgc tcactttgtg ccgccaacgg taacggaaga gagcatcgtc
    33661 tggccggaag gaccacgccc accgtcgcgt gactatacgc cactgtatga cgaactacgc
    33721 catgaactgg cgatcgattt ctttattcac gacggtgggg tcgccagcgg ctgggcgata
    33781 aacttacggt ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc
    33841 tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca
    33901 aacttgccgt taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg
    33961 attatctcgc gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg
    34021 tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg
    34081 gcgaaggtaa agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac
    34141 agcgggtacg tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg
    34201 ctgacgattt ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg
    34261 accaagcgat tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt
    34321 ataaagtaac tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa
    34381 tgtattttca atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag
    34441 gatattttgg ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc
    34501 gccgggactg gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag
    34561 gccgggttta ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt
    34621 tgtggcatca ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag
    34681 gagctgcacg caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg
    34741 ttggcgattc tgggcaaaca agcatatgaa cagggattca gctagcgcgg tgcacagtgg
    34801 gggaaacaaa cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt
    34861 ttaagtcgcg tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg
    34921 gtagtgcgtg ggcgataa
//
LOCUS       DAEBXD010000036        34086 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEBXD010000036 DAEBXD010000000
VERSION     DAEBXD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34086
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.036"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..578
                     /locus_tag="KJL94_001813"
     CDS             <1..578
                     /locus_tag="KJL94_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4410191.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="KJL94_001814"
     CDS             complement(575..1711)
                     /locus_tag="KJL94_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4410192.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="KJL94_001815"
     CDS             complement(1717..2040)
                     /locus_tag="KJL94_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD4410193.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="KJL94_001816"
     CDS             2223..2366
                     /locus_tag="KJL94_001816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410194.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KJL94_001817"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KJL94_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBD4410195.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="KJL94_001818"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="KJL94_001818"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBD4410196.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="KJL94_001819"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="KJL94_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBD4410197.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="KJL94_001820"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="KJL94_001820"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBD4410198.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="KJL94_001821"
     CDS             complement(8959..9567)
                     /locus_tag="KJL94_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBD4410199.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="KJL94_001822"
     CDS             complement(9638..10723)
                     /locus_tag="KJL94_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4410200.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="KJL94_001823"
     CDS             complement(10726..11088)
                     /locus_tag="KJL94_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD4410201.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="KJL94_001824"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="KJL94_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBD4410202.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="KJL94_001825"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="KJL94_001825"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD4410203.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="KJL94_001826"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="KJL94_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBD4410204.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="KJL94_001827"
     CDS             complement(15380..15589)
                     /locus_tag="KJL94_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410205.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="KJL94_001828"
     CDS             15873..16235
                     /locus_tag="KJL94_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBD4410206.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="KJL94_001829"
     CDS             16779..17462
                     /locus_tag="KJL94_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD4410207.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="KJL94_001830"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="KJL94_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="KJL94_001831"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="KJL94_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBD4410208.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="KJL94_001832"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="KJL94_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBD4410209.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="KJL94_001833"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="KJL94_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBD4410210.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="KJL94_001834"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="KJL94_001834"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBD4410211.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="KJL94_001835"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="KJL94_001835"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBD4410212.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="KJL94_001836"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="KJL94_001836"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBD4410213.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="KJL94_001837"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="KJL94_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBD4410214.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="KJL94_001838"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="KJL94_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBD4410215.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="KJL94_001839"
     CDS             complement(29422..29853)
                     /locus_tag="KJL94_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBD4410216.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="KJL94_001840"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="KJL94_001840"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBD4410217.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="KJL94_001841"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="KJL94_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBD4410218.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="KJL94_001842"
     CDS             32939..33898
                     /locus_tag="KJL94_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBD4410219.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34086)
                     /locus_tag="KJL94_001843"
                     /pseudo
     CDS             complement(33885..34086)
                     /locus_tag="KJL94_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8470 a         8929 c         8690 g         7997 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccgga taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata atcatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcg
//
LOCUS       DAEBXD010000037        33546 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEBXD010000037 DAEBXD010000000
VERSION     DAEBXD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33546
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.037"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            78..1355
                     /gene="waaA"
                     /locus_tag="KJL94_001844"
     CDS             78..1355
                     /gene="waaA"
                     /locus_tag="KJL94_001844"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBD4410220.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1363..1842
                     /gene="coaD"
                     /locus_tag="KJL94_001845"
     CDS             1363..1842
                     /gene="coaD"
                     /locus_tag="KJL94_001845"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBD4410221.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KJL94_001846"
     CDS             complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KJL94_001846"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBD4410222.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KJL94_001847"
     CDS             complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KJL94_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBD4410223.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KJL94_001848"
     CDS             complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KJL94_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBD4410224.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KJL94_001849"
     CDS             complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KJL94_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBD4410225.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4269..5489
                     /gene="coaBC"
                     /locus_tag="KJL94_001850"
     CDS             4269..5489
                     /gene="coaBC"
                     /locus_tag="KJL94_001850"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBD4410226.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5467..5925
                     /gene="dut"
                     /locus_tag="KJL94_001851"
     CDS             5467..5925
                     /gene="dut"
                     /locus_tag="KJL94_001851"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBD4410227.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6032..6628
                     /gene="slmA"
                     /locus_tag="KJL94_001852"
     CDS             6032..6628
                     /gene="slmA"
                     /locus_tag="KJL94_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBD4410228.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KJL94_001853"
     CDS             complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KJL94_001853"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBD4410229.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KJL94_001854"
     CDS             complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KJL94_001854"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBD4410230.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8215..9078
                     /locus_tag="KJL94_001855"
     CDS             8215..9078
                     /locus_tag="KJL94_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBD4410231.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9299..10123
                     /gene="dinD"
                     /locus_tag="KJL94_001856"
     CDS             9299..10123
                     /gene="dinD"
                     /locus_tag="KJL94_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBD4410232.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10414..11031
                     /locus_tag="KJL94_001857"
     CDS             10414..11031
                     /locus_tag="KJL94_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBD4410233.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KJL94_001858"
     CDS             complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KJL94_001858"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBD4410234.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12968..13591
                     /gene="gmk"
                     /locus_tag="KJL94_001859"
     CDS             12968..13591
                     /gene="gmk"
                     /locus_tag="KJL94_001859"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBD4410235.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13646..13921
                     /gene="rpoZ"
                     /locus_tag="KJL94_001860"
     CDS             13646..13921
                     /gene="rpoZ"
                     /locus_tag="KJL94_001860"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBD4410236.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13940..16048
                     /gene="spoT"
                     /locus_tag="KJL94_001861"
     CDS             13940..16048
                     /gene="spoT"
                     /locus_tag="KJL94_001861"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBD4410237.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16055..16744
                     /gene="trmH"
                     /locus_tag="KJL94_001862"
     CDS             16055..16744
                     /gene="trmH"
                     /locus_tag="KJL94_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBD4410238.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16750..18831
                     /gene="recG"
                     /locus_tag="KJL94_001863"
     CDS             16750..18831
                     /gene="recG"
                     /locus_tag="KJL94_001863"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBD4410239.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KJL94_001864"
     CDS             complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KJL94_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBD4410240.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20350..21741
                     /gene="xanP"
                     /locus_tag="KJL94_001865"
     CDS             20350..21741
                     /gene="xanP"
                     /locus_tag="KJL94_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBD4410241.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21862..23571
                     /locus_tag="KJL94_001866"
     CDS             21862..23571
                     /locus_tag="KJL94_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD4410242.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KJL94_001867"
     CDS             complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KJL94_001867"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBD4410243.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25952..27335)
                     /locus_tag="KJL94_001868"
                     /pseudo
     CDS             complement(25952..27335)
                     /locus_tag="KJL94_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27628..27722
                     /locus_tag="KJL94_001869"
     tRNA            27628..27722
                     /locus_tag="KJL94_001869"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27662..27664,aa:Sec,seq:tca)
     gene            28023..29207
                     /locus_tag="KJL94_001870"
     CDS             28023..29207
                     /locus_tag="KJL94_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4410244.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29619..30662
                     /locus_tag="KJL94_001871"
     CDS             29619..30662
                     /locus_tag="KJL94_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBD4410245.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31177..31605
                     /locus_tag="KJL94_001872"
                     /pseudo
     CDS             31177..31605
                     /locus_tag="KJL94_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31635..32786)
                     /locus_tag="KJL94_001873"
                     /pseudo
     CDS             complement(31635..32786)
                     /locus_tag="KJL94_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32851..33505
                     /locus_tag="KJL94_001874"
                     /pseudo
     CDS             32851..33505
                     /locus_tag="KJL94_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8406 a         8816 c         8266 g         8058 t
ORIGIN      
        1 aaaaacggga aaagtaatgg taaagccaca gctaaataca tagaatcccc agcacatcca
       61 taagtcagct atttactatg ctcgaattgc tttacaccgc ccttctctac cttattcagc
      121 cgctgatctg gatacggctc tgggtgcgcg gacgtaaggc tccggcctat cgaaaacgct
      181 ggggtgaacg ttacggtttt taccgccatc cgctaaaacc aggcggcatt atgctgcact
      241 ccgtctccgt cggtgaaact ctggcggcga tcccgttggt gcgcgcgctg cgtcatcgtt
      301 atcctgattt accgattacc gttacaacca tgacgccaac cggttcggag cgcgtacaat
      361 cggctttcgg gaaggatgtt cagcacgttt atctgccgta tgacctgccc gatgcgctca
      421 accgtttcct gaataaagtc gaccccaaac tggtgttgat tatggaaacc gaactatggc
      481 ctaacctgat tgcggcgcta cataaacgta aaattccgct ggtgatcgct aacgcgcgac
      541 tctctgcccg ctcggccgca ggttatgcca aactgggtaa attcgtccgc cgcttgctgc
      601 gtcgtattac gctgattgct gcgcaaaatg aagaagatgg tgcacgtttt gtggcgctgg
      661 gcgcaaaaaa taaccaggta accgttaccg gtagcctgaa attcgatatt tctgtaactc
      721 cgcagttggc tgctaaagcc gtgacgctac gccgccaatg ggcaccacac cgcccggtat
      781 ggattgccac cagcactcat gaaggtgaag agagcgtggt cattgccgca catcaggcat
      841 tgttacagca attcccgaat ttattgctca tcctggtacc ccgtcatcca gaacgcttcc
      901 cggatgcgat taaccttgtc cgccaggctg gactaagcta tatcacacgc tcttcagggg
      961 aagtcccctc caccagcacg caggttgtgg ttggcgatac gatgggcgag ttgatgttac
     1021 tgtacggcat tgccgatctc gcctttgttg gcggttcact ggttgaacgt ggtgggcata
     1081 atccgctgga agctgccgca cacgctattc cggtattgat ggggccgcat acttttaact
     1141 ttaaagacat ttgcgcgcgg ctggagcagg caagcgggct tattaccgtt accgatgcca
     1201 ctacgcttgc aaaagaggtt tcctctttac tcaccgacgc cgtttaccgt agtttctatg
     1261 gccgtcatgc cgttgaagta ctgtatcaaa accagggcgc gctacagcgt ctgcttcaac
     1321 tgctggaacc ttacctgcca ccgaaaacgc attgaggttg ttatgcaaaa acgggcgatt
     1381 tatccgggta ctttcgatcc cattaccaat ggtcatatcg atatcgtgac gcgcgccacg
     1441 cagatgttcg atcacgttat tctggcgatt gccgccagcc ccagtaaaaa accgatgttt
     1501 accctggaag agcgtgtggc actggcacag caggcaaccg cgcatctggg gaacgtggaa
     1561 gtggtcgggt ttagtgattt gatggcgaac ttcgcccgta atcaacacgc tacggtgctg
     1621 attcgtggcc tgcgtgcggt ggcggatttt gaatatgaaa tgcagctggc gcatatgaat
     1681 cgccacttaa tgccggaact ggaaagtgtg tttctgatgc cgtcgaaaga gtggtcgttt
     1741 atctcttcat cgttggtgaa agaggtggcg cgccatcagg gcgatgtcac ccatttcctg
     1801 ccggagaatg tccatcaggc gctgatggca aaattagcgt agcgtttatg ccggatggta
     1861 tgccatccgg ctcgcatgaa ttacttctgg cactgccgac aataaaacgt tgcccgctgc
     1921 gcatgtttag tcgccacaat cggcgtaccg cacacccgac acggctcacc ttttcgcccg
     1981 taaacctgca attcctgcgc gaaatagccc ggtttaccat cactttgcag aaaatctttc
     2041 agcgttgtac caccctgctc aatcgaacgc agcaacaccg ctttaatcac ccgagctaac
     2101 aattcacact ctgccagcga cagtgatgac gccagccgat ccggatggat ccccgccgca
     2161 aacagtgatt cgctggcata gatattccct acccctacca ccagcttgtt atccatcagc
     2221 cacggtttaa tcgccgtttt tttcttcgcg cacttctgat gcagatactc accattgaaa
     2281 tcgtcgctaa gcggctccgg tccaagatgg gccagcacat tatgcccttc cagctctttg
     2341 gtccacagcc aggcaccaaa gcggcgcgga tcggtgtagc gcagcacttt accgttgctc
     2401 atcaccaaat ccacatggtc atgcttttca gggggaagtt cttctggaag gatgcgcagg
     2461 ctgccagaca tccccaaatg aatgataatc cagccctcag gcagctccag cagcagatat
     2521 ttagcccgcc gctgcacgct aagcactggt tgatcgctta atcggtagat ctcttctgaa
     2581 accggccagc gcaagcgtcc gttgcgcacc accgcatgaa gaatggttgc accaacgaga
     2641 tgcggttcta tgccgcgacg gctggtttca acttcgggta attcaggcat agcatctcca
     2701 ggaatgaaca gatgcaatca atatgggggc aagcagataa caaaaaaccc cgccggagcg
     2761 aggtttttcg ttacatcaaa gcgagaatta tttgattttc gcttctttgt agatcacgtg
     2821 ctgacgaaca actggatcga attttttcag ttccagtttt tccggcttag tacgtttgtt
     2881 cttcgtagtg gtatagaagt gaccagtacc agcagaagaa accagcttga ttttctcacg
     2941 aataccttta gccatgattt atttcctcta agtacttagt acttttcgcc acgggcacgc
     3001 agttcagcca gaactgtatc gatgcctttt ttatcgatta cacgcatacc tttagcagat
     3061 acgcgcaggg tgacaaaacg cttctcgctc tcaacccaga aacggtgaga gtgcaggttc
     3121 ggcaggaaac ggcgtttagt cgcgttcagt gcgtgggaac ggttgttacc ggtcaccgga
     3181 cgcttgccag taacttggca gactcgggac atgtctattc tccaaaaatc aaattagctc
     3241 gagcttcgta tggggtattg gcgcctcgtc aggctttaca gcccggtcat cgcagttcta
     3301 tgtgaactct cgattgccag gcccaaatgc caaacccgag attctcaaag gtggcgtagt
     3361 atacgctgac tcagcgatgt gctcaagtcc cgaacagaca aagatcccga aggatcgcgc
     3421 atagcgggtt aaatccagcc gcgttcggca aaagaaacat actctccacg cccaatcacg
     3481 atatggtcga gcacgcgtaa atccatgaac tgacaactct ttattatccg ttcagtaatg
     3541 agtttatccg ctttactggg ttcagcacaa cccgaagggt gattatgtgc aaggatcagc
     3601 gccgaggcgt ttatttttat cgcttcgcgg ataatttccc gaggatggac ttcaacatgg
     3661 tttagcgtgc cggaaaaaag acggctatgc gttataaccc ggtgttgtga gtcgagaaag
     3721 atcaccataa agatctcccg ctcctcaccc gtgagctggc tttgtaaaaa ttcccgcgtc
     3781 atctccgggc tgagtaaagg gctttcttca cgcatccgca cgttgtagta acgtcgcgcc
     3841 agttcagcaa tcccctttaa ctgggcaaat ttcgctacgc caattccatg aacaccacta
     3901 aattgttcat attcagaggt taacaagcca taaagagagc cgaaattctc cagcatctct
     3961 tttgccaggg ttaatacatc tttaccgcgc gttccggtac gcagaaatag cgccagcagc
     4021 tcgacatccg ttaaggcgct aataccaaac ttcagcattt tttcgcgcgg catcaacagc
     4081 tgtgcattgt ttttcacctt cacctccttt gtggtgctcg catcctgtca tagccgttcc
     4141 gttaaatcga cggccagttt tcaatcctgg aaagagcctc gcaaagtgaa tctcaggtga
     4201 aggcacgaca acaaaagtat tgtgataaaa tcgccaactt ctggtgtcac ccaacaggaa
     4261 aaatcatcat gagcctggcc ggtaaaaaaa tcgttctcgg cgttagcggc ggtattgctg
     4321 cctataaaac ccctgaactg gtgcgtcgtt tgcgcgatcg cggggccgac gtccgcgtag
     4381 ccatgaccga agcggcaaaa gcctttatca ccccacttag cttgcaggcg gtttctggtt
     4441 atcccgtttc cgacagtctg cttgacccgg cagccgaagc cgctatgggc catattgagc
     4501 tgggtaaatg ggctgattta gtgattctcg cccctgccac ggcagatttg attgcccgtg
     4561 ttgctgccgg aatggcgaat gacctggtat cgacgatttg tctggctaca cctgcgcctg
     4621 tagccgtgct ccccgccatg aaccagcaga tgtaccgtgc cgctgccacg cagcataatt
     4681 tagaggtgct tgcttcccgt ggtttgctca tctgggggcc agacagtggc agtcaggctt
     4741 gtggtgatat cggtcctgga cgaatgctcg atccgttaac cattgtggat atggcggtgg
     4801 cgcatttttc gcccgtcaac gacctgaaac atctgaacat tatgattacc gccggtccga
     4861 cgcgtgaacc gctcgatccg gtgcgttata tctctaatca cagctccggc aagatgggtt
     4921 ttgctatcgc cgccgctgct gcccgtcgtg gcgcgaacgt cacgctggta tcaggtccag
     4981 tttcactacc gacgccaccg tttgttaaac gtgttgatgt gatgaccgcg ctggaaatgg
     5041 aagccgccgt gaatgcttct gtacagcagc aaaatatttt tatcggttgc gccgccgtgg
     5101 cggattatcg cgcagctacc gtggccccag aaaaaatcaa aaagcaggcc acgcagggtg
     5161 atgaattaac aataaaaatg gttaaaaacc ccgatatcgt cgcaggcgtt gccgcactaa
     5221 aagaccatcg accctacgtc gttggatttg ccgccgaaac aaataatgtg gaagaatacg
     5281 cccggcaaaa acgtatccgt aaaaaccttg atctgatctg cgcgaacgat gtttcccagc
     5341 caactcaagg atttaacagc gacaacaacg cattacacct tttctggcag gacggagata
     5401 aagtcttacc gcttgagcgc aaagagctcc ttggccaatt attactcgac gagatcgtga
     5461 cccgttatga tgaaaaaaat cgacgttaag attctggacc cgcgcgttag gaaggaattt
     5521 ccgctcccga cttatgccac ctctggctct gccggacttg acctgcgtgc ctgtctcgac
     5581 gacgccgtag aactggctcc gggtgacact acgctggttc cgaccgggct ggcgattcat
     5641 attgccgatc cttcactggc ggcaatgatg ctgccgcgct ccggattggg acataagcac
     5701 ggtatcgtgc ttggtaacct ggtaggattg atcgattccg actatcaggg ccagttgatg
     5761 atttccgtgt ggaaccgtgg tcaggacagc ttcaccattc aacctggcga acgcatcgcc
     5821 cagatgattt ttgttccggt agtacaggct gaatttaatc tggtggaaga tttcgacgcc
     5881 accgaccgcg gtgaaggcgg ctttggtcac tctggtcgtc agtaacacat acgcatccga
     5941 ataacgtcat aacatagccg caaacatttc gtttgcggtc atagcgtggg tgccgcctgg
     6001 caagtgctta ttttcagggg tattttgtaa catggcagaa aaacaaactg cgaaaaggaa
     6061 ccgtcgcgag gaaatacttc agtctctggc gctgatgctg gaatccagcg atggaagcca
     6121 acgtatcacg acggcaaaac tggccgcctc tgtcggcgtt tccgaagcgg cactgtatcg
     6181 ccacttcccc agtaagaccc gcatgttcga tagcctgatt gagtttatcg aagatagcct
     6241 gattactcgc atcaacctga ttctgaaaga tgagaaagac accacagcgc gcctgcgtct
     6301 gattgtgttg ctgcttctcg gttttggtga gcgtaatcct ggcctgaccc gcatcctcac
     6361 tggtcatgcg ctaatgtttg aacaggatcg cctgcaaggg cgcatcaacc agctgttcga
     6421 gcgtattgaa gcgcagctgc gccaggtatt gcgtgaaaag agaatgcgtg agggtgaagg
     6481 ttacaccacc gatgaaaccc tgctggcaag ccagatcctg gccttctgtg aaggtatgct
     6541 gtcacgtttt gtccgcagcg aatttaaata ccgcccgacg gatgattttg acgcccgctg
     6601 gccgctaatt gccgcgcagt tgcagtaata tgacgccgga tgacttttca tccggcgagt
     6661 ttctttaaac gccaaactct tcgcgatagg ccttaaccgc cgccagatgt tccgccattt
     6721 ccggcttctc ttccagatag gcaatcagat ctttcagggt gatgatagag atcactttgc
     6781 agttgtaatc acgctcaact tcctgaatcg ccgaaatctc gccgcgcccg cgttcctgac
     6841 gatcgagcga aatcaacacg ccagcaagcg tcgcgccatt ggcctgaata atctccatcg
     6901 actcgcgaat cgccgttccg gcggtgatca catcatctac cagcattacg cgtccttgta
     6961 acgcgctacc aaccagattg ccgccttcac cgtggtcttt tgcttctttg cggttaaagc
     7021 agtacggcag gtccaggtcg tgatgctccg ccagtgccac agcggttgtg gtggcaatcg
     7081 ggatcccttt gtaagcaggg ccaaacagca gatcgaactc aatgccggaa tccaccaacg
     7141 cttcagcgta aaaacggcct aacagtgcca gatcgcgccc ggtattaaac agcccggcgt
     7201 tgaagaaata ggggcttttg cgcccggatt tcagcgtaaa ctcgccaaac tttaacacct
     7261 gcttgctaag cgcaaattca ataaactggc gctgatatgg tttcatgcct tcgctcctca
     7321 tcttactttt ctacagacaa aaaaaaggcg actcatcagt cgccttaaaa atcagtttgc
     7381 cagcgccgcc ttctgcgtcg ctacaatgga ttcgattccc cctcgggcca gcgccaacaa
     7441 ggtgagtagc tcttcatggg tgaacggctc gccttctgcc gtcccctgca cttcaatgat
     7501 gcgcccgtct tcggtcatca ctacgttcat gtcggtctct gcggctgagt cttcaacgta
     7561 ttccagatcg caaattgctt caccgtttac aataccgaca gaaactgcgg ctaccatccc
     7621 tttcatcggg ttggttttca gcttgccgtt ttccaccagc ttctgtagcg catctgccag
     7681 cgccacgcag gcacccgtaa tcgacgcggt acgagtgcca ccatcagcct gaagcacgtc
     7741 gcagtccagc gtaatggtga actcacccag cgctttcaaa tctactgccg cgcgaagagc
     7801 acgggcgatc agacgctgga tttccattgt gcgtccaccc tgcttacctt tcgccgcttc
     7861 acgagcgtta cgggtgtggg tagaacgtgg cagcatgccg tactctgcgg tgatccagcc
     7921 ctggccctga cctttcagga agcgcggcac gccttcttca atagaggcgg tacacaatac
     7981 tttggtatcg ccaaattcga ccagcaccga gccttctgca tgttttgtat agttacgagt
     8041 cagggtaacg ggacgcacct gattattgct acggcctgct ggacgcatat tgaaatctcc
     8101 ggcttgaaac aaatgtggct gcgcattata cggacttccg gcggttattc ctatcctgac
     8161 aaggcatcga tggctataat ccttccacct ctccttttat aaacaggaac gtctatgatc
     8221 cgcagtatga ccgcctacgc ccggcgtgaa atcaagggtg aatgggggag cgcaacctgg
     8281 gaaatgcgct cggtaaacca gcgttatctg gaaacttact ttcgtctgcc ggagcagttc
     8341 cgtagccttg aacctgtcgt tcgcgagcgt attcgttctc gcctgacgcg cggtaaagtg
     8401 gaatgtaccc tgcgctatga gccagatgtt agcgcacaag gtgagctgat cctcaatgaa
     8461 aaactggcta aacagctggt aactgccgcg aactgggtaa aaatgaagag tgacgaaggg
     8521 gaaatcaacc cggttgatat tctacgctgg ccgggcgtga tggcagccca ggagcaggat
     8581 ctcgacgcga ttgccgctga aattctcgcg gcgttggatg gtacgctgga cgactttatc
     8641 gtcgcgcgcg aaaccgaagg tcaggcactg aaagcattga tcgagcagcg tctggaaggc
     8701 gtcaccgccg aagtggtcaa agtccgcgcc catatgccgg aaattctgca atggcagcgt
     8761 gagcgtctgg tcgcgaagct ggaagatgct caggttcagt tggaaaacaa ccgtctggag
     8821 caggagttgg ttctgctggc acaacgaatt gacgttgccg aagagctgga ccgcctcgaa
     8881 gcgcatgtta aagagacgta caacattctg aagaaaaaag aagcggttgg tcgtcgtctg
     8941 gactttatga tgcaggagtt caaccgtgag tcgaacactc ttgcgtcgaa atctatcaat
     9001 gccgaagtga caaactccgc catcgagctg aaagtgttga ttgagcagat gcgcgagcag
     9061 attcagaata ttgagtaact cgtaacacca attctgactt ttcagccttc acaaatgccg
     9121 cccactcaaa cagagcggca tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg
     9181 ctccagattt tgcgatgttt ttttgcccag cacactgaga acgtgagata ctcacaactg
     9241 tatataaata cagttacaga tttactttct ttgcaatcga tatcacatgg agtgggcaat
     9301 gaacgaacat catcaacctt ttgaagagat aaaactgatt aatgcaaacg gagcagaaca
     9361 atggtcagca agacaacttg ggaaactact gggttattca gagtatcgtc actttatacc
     9421 tgtattaacg cgcgccaaag aggcctgtga gaacagtggt cacacaattg atgaccattt
     9481 cgaggagatc ctcgatatgg tcaaaattgg ctcaaatgcc aaaagagcat taaaagacat
     9541 cgtactctcc cgctatgcct gttacctggt agtacaaaac ggcgaccccg cgaaaccggt
     9601 cattgcggca gggcagactt attttactat ccagacccga cggcaggagc ttgctgacga
     9661 tgaagcattt aagcaacttc gtgaagacga aaagcgtctg tttctgagaa atgagttgaa
     9721 agaacataac aaacaattgg ttgaggcagc acagcaagct ggggtagcga cagctactga
     9781 tttcgccata tttcagaatc atggttacca ggggctgtat ggtggattag atcagaaagc
     9841 catccaccaa ctgaaggggc tgaaaaagag tcagaagatc ctggatcata tgggttcaac
     9901 agaactggcg gctaatctct ttcgagctac ccaaacagaa gaaaaactca agcgggatag
     9961 cgtgaattca aaacagcaag ctaataccac acactttgac gtgggtagca aggtgaggca
    10021 aaccattcag gaacttggcg gaaccatacc tgaggagttg ccgaccccgc aggtcagcat
    10081 caagcagttg gaaaacagtg taaaaattac agagaagaaa tagcagaaac gaaaaactct
    10141 ttttgttgat tgagacaccc gatgcgtaag ccaaggtcca ggtgcagtga acacaatggc
    10201 taaacattgc acctttcttt tcccccctca gttttaacct attttttctt atgcattttc
    10261 tcagacaaga agtcagaaga atgcatctac gctacagaaa atagcgattt cacataacta
    10321 aaagttatca atttcctctc accttaaaaa atatcaatcg tgacaatgcg cacaaatcgc
    10381 taccctgcca gacagatttt tagggagaga accatgctgt tacacatttt gtatttggtt
    10441 ggcattactg ccgaagccat gacgggggcg ctggcggccg ggcgacggcg catggataca
    10501 tttggcgtaa ttattattgc aaccgccacc gcaattggcg gagggtcagt gcgcgatatt
    10561 ctgctgggcc actatccgct cggctgggtc aaacacccgg aatatgtgat tatcgtcgct
    10621 accgccgcag tactcaccac tatcgttgct cctgtaatgc cttacctgcg caaagtgttt
    10681 ctggtgctcg atgcgctcgg actggtggtc ttttctatca tcggcgcaca ggttgcactg
    10741 gatatggggc acggcccaat tattgccgtt gtcgcggcgg taaccaccgg tgtatttggc
    10801 ggcgttttgc gcgatatgtt ctgtaaacgc attccgctgg tctttcaaaa agagttgtat
    10861 gccggtgtct catttgcctc cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac
    10921 catgatgtgg tgattatctc caccctggta ttcggctttt ttgctcgttt actggcgcta
    10981 cgtcttaagc tgggattacc ggtcttttac tacagccacg aaggtcacta aggttcaaaa
    11041 cctgtgatct gctgggcagc cagccaactg cccagcttct tgatttgcgc attttccttc
    11101 cattcaataa cctgtctggc gcgcccagat ccagtccccg gcagctgctg ccagaactgc
    11161 tccgtgctaa ataaaagttg cgaccaggac cgttcatcac tggcattaag cgccgcccgg
    11221 gttagcggta ttcccattgc catcacccag cgagtaaaag gctggttacg agccagatta
    11281 aactgatgcc ataactgcgc acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc
    11341 tctggcgtta ataaaagcca ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg
    11401 cgccaaccgg cctcaccaat gccatccagc cccagaacct gttttgaccc cagccagact
    11461 aagcgtgaaa tgaactgttc ctgacaaaca tcagaagcaa agtagcaggt caacgggtta
    11521 aagcggtttt ctggtggtgt cggttttgta cgttctgcac cgcgccagac cacatcatca
    11581 atgcgaggaa taccctgacc ggcaaggctg acgaggatct gatcaccagg cgcaatatcc
    11641 cactcctgcc agcgcctgac ggaaccaata ttcacccgct ggactttttt atcatccagc
    11701 atgacaggtg cgagtgacgc aaccaccgat attttaccgc tcttacccac cgcaaactga
    11761 atcgccttca cttcggcaac ctgagctaca ggttgatatt tccaggccac cagccactcc
    11821 gcctggcccg gtagccaatg acgggattcg ggttccttag cccctcgcac aaccacgcca
    11881 tcggtgacga agggtaattt cgctttccac cactcattgc gtacgcgcgc aacctcatca
    11941 gcatttttaa ccgcacgggt atacctctgc gttagagtaa aacctgcggt agccagctcc
    12001 tttaaacgat cggtcattaa ctgcggtcca tccggccatg cccagacaaa aacagccagt
    12061 gagtttagcg tgtcgctatt cccctggcgc atcatcaggc cagcaacttt tgcgcgggca
    12121 tttattcccc ccatttgttg ttggatatgc ccctcgcgct ggagaaatat ttccccctga
    12181 agcgtactgt tggctaaagg cccgctaacg gtttgcggca cagcggaaat taagctaact
    12241 ttctgcgtcc agtcctcgcc tttcaggcca ttaccgcgac tgattgcttt gttcagtttc
    12301 ccgtcccgat aaaccagggt taccgcaacg ccatcaactt ttggctgcac ccaaagatcg
    12361 ctacgttctc gcatccacag acttaatgcg tttttatccg ccattttgcg tacacccgta
    12421 tgggcaaccg gatgcataac cgcaccattt aaaggcggca tcatcacgtc gcggggctcg
    12481 ctcacaaagc agcgttgcca ctgcgtaaga cgggcgctta actgatcgta aacaccgtct
    12541 tccacctcac tttttccttc cttccagtag tcatcgtccc actgttttat ttgctgttgc
    12601 aggcgggaaa tttcttcctg tgctctggct ggcgaccagg ccggacagac cgcccacaca
    12661 gatgattgcc agcacaagat acttattaat atcgccatcc atactttcat cttcacctcc
    12721 gctgtagtca ggctggcaga tatacaacgt ggtgaaggca aagccgagtg gcaaaaacgg
    12781 agtctgcgag gacgcttcct gagaatcgtc tttattgcag taaatcacag gcaaatgcgg
    12841 aaacagctac gcaaaacgca acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca
    12901 tggccgcgcc gtgtataata agctcgtatg taggctttat ttcgctaatc acatacgaaa
    12961 gatactcatg gctcaaggca cgctttatat tgtttctgcc cccagtggcg cgggtaaatc
    13021 cagcctgatt caggctttat taaaaaccca accgttgtat gacacccagg tttctgtttc
    13081 acacaccaca cgccaaccgc gtcctggtga agtccacggt gaacattatt tctttgttaa
    13141 tcatgatgaa tttaaagaaa tgattagcag agatgcgttc ctcgaacacg cagaagtttt
    13201 tggtaattac tatggcactt cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga
    13261 tgtttttctc gatatcgact ggcagggcgc gcagcaaatt cgccagaaga tgccgcacgc
    13321 gcggagtatc tttattttac cgccgtccaa aattgaactg gaccgccgtc tacgcggtcg
    13381 cggtcaggac agcgaagagg tcattgcaaa gcgtatggcg caagctgttg cagaaatgag
    13441 ccattacgcc gaatatgatt atctgattgt gaatgatgac ttcgataccg cgttgaccga
    13501 tttgaagacc attattcgcg ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga
    13561 cgctttaatc agcaaattgt tggcagactg aacctgattt cagtatcatg cccagtcatt
    13621 tcttcacctg tggagctttt taagtatggc acgcgtaact gttcaggacg ctgtagagaa
    13681 aattggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt
    13741 aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg
    13801 cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac gccaggaaca
    13861 gcaagagctg gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtta
    13921 atcacaaagc gggtcgccct tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta
    13981 cctgccggaa gaccaaatca agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca
    14041 cgaggggcaa acacgttcaa gcggtgaacc ctatatcacg cacccggtag cggttgcctg
    14101 cattctggcc gagatgaaac tcgactatga aacgctgatg gcggcgctgc tgcatgacgt
    14161 gattgaagat actcccgcca cctaccagga tatggaacag ctttttggta aaagcgtcgc
    14221 cgagctggta gagggggtgt cgaaacttga taaactcaag ttccgcgata agaaagaggc
    14281 gcaggccgaa aactttcgca agatgattat ggcgatggtg caggatatcc gcgtcatcct
    14341 catcaaactt gccgaccgta cccacaacat gcgcacgctg ggctcacttc gcccggacaa
    14401 acgtcgccgc atcgcccgtg aaactctcga aatttacagc ccgctggcgc accgtttagg
    14461 tatccaccac attaaaaccg aactcgaaga gctgggtttt gaggcgctgt atcccaaccg
    14521 ttatcgcgta atcaaagaag tggtgaaagc cgcgcgcggc aaccgtaaag agatgatcca
    14581 aaaaatcctc tcggaaatcg aagggcgttt gcaggaagcg ggaattccct gccgcgtcag
    14641 tggtcgcgag aagcatcttt attcgattta ctgcaaaatg gtgctcaaag agcagcgttt
    14701 tcactcaatc atggacatct acgctttccg cgtgatcgtc aatgattccg acacctgtta
    14761 tcgcgtgctc gggcaaatgc atagcctgta caagccgcgt ccgggtcgcg tgaaagacta
    14821 catcgccatt ccaaaagcga acggctatca gtctttgcac acctcgatga tcggcccgca
    14881 cggtgtgccg gttgaggtcc agatccgtac cgaagatatg gatcagatgg cggagatggg
    14941 tgttgccgcg cactgggctt ataaagagca cggcgaaacc agcaccaccg cgcaaatccg
    15001 cgcccagcgc tggatgcaaa gcctgctgga gctgcaacag agcgccggta gttcgtttga
    15061 atttatcgag agcgttaaat ccgatctctt cccggatgag atttacgttt tcacaccgga
    15121 agggcgaatt gtcgagctgc ctgccggtgc aacgcccgtc gacttcgctt atgcagtgca
    15181 taccgatatc ggtcatgcct gcgtgggcgc acgcgttgac cgccagcctt acccgctgtc
    15241 gcagccgctt accagcggtc aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa
    15301 tgccgcgtgg ctgaactttg tcgttagctc aaaagcgcgc gccaaaattc gtcagttgct
    15361 gaaaaacctc aagcgtgatg attctgtaag cctgggccgt cgtctgctca accatgcttt
    15421 gggtggtagc cgtaagctga atgaaatccc gcaggaaaat attcagcgcg agctggatcg
    15481 catgaagctg gcaacgcttg acgatctgct ggcagaaatc ggacttggta acgcaatgag
    15541 cgtggtggtc gcgaaaaatc tgcaacatgg ggacgcctcc attccaccgg caacccaaag
    15601 ccacggacat ctgcccatta aaggtgccga tggcgtgctg atcacctttg cgaaatgctg
    15661 ccgccctatt cctggcgacc cgattatcgc ccacgtcagc cccggtaaag gtctggtgat
    15721 ccaccatgaa tcctgccgta atatccgtgg ctaccagaaa gagccagaga agtttatggc
    15781 tgtggaatgg gataaagaga cggcgcagga gttcatcacc gaaatcaagg tggagatgtt
    15841 caatcatcag ggcgcgctgg caaacctgac ggcggcaatt aacaccacga cttcgaatat
    15901 tcaaagtttg aatacggaag agaaagatgg tcgcgtctac agcgccttta ttcgtctgac
    15961 cgctcgtgac cgtgtgcatc tggcgaatat catgcgcaaa atccgcgtga tgccagacgt
    16021 gattaaagtc acccgaaacc gaaattaatg ttttatgaac ccaacacgtt atgcacgcat
    16081 ctgcgaaatg ctcgccaggc ggcagcctga tctgaccgtc tgcatggagc aggtccacaa
    16141 acctcataac gtttctgcga ttattcgtac cgcagatgcc gttggcgtac atgaagttca
    16201 cgccgtctgg cctggtagcc gcatgcgcac catggcttcg gcagcggcgg gtagtaacag
    16261 ctgggtacag gtgaaaacac accgcaccat tggcgatgcc gtcgctcatc tcaaaggcca
    16321 gggcatgcag attctggcaa cccatctttc tgataatgct gtcgatttcc gcgaaattga
    16381 ttacactcgc ccgacctgca ttttgatggg acaggagaaa acgggcatca cgcaggaagc
    16441 attggccctg gcggatcagg acatcatcat tccgatgatc ggcatggtgc agtcgctgaa
    16501 tgtttccgtt gcctcagccc tcattcttta cgaagcccag cgtcagcggc aaaatgcagg
    16561 catgtacctg cgtgaaaaca gcatgttgcc ggaagcagag caacaacgcc tgttgtttga
    16621 aggcggctat ccggtgctgg cgaaagtcgc aaaacgcaaa ggcctgcctt atccccacgt
    16681 caatcagcaa ggcgagatcg aagctgatgc cgactggtgg gctactatgc aggctgcagg
    16741 gtaagtgcca tgaaaggtcg cctgttagat gctgtcccac tcagttccct aacgggcgtt
    16801 ggcgcagcac ttagtaacaa actggcgaaa atcaacctgc ataccgtgca ggatctactc
    16861 ttacaccttc ccctgcgcta cgaagatcgc acccatctct accccatcgg agaactactg
    16921 ccgggcgttt atgccacggt ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt
    16981 cggcggatga tgacctgcca gatcagcgac ggttccggca tcctcaccat gcgctttttc
    17041 aatttcagcg cggcaatgaa aaatagcctg gcgacgggcc gccgtgtact ggcttatggc
    17101 gaagcaaagc gcggtaaata tggtgcggag atgatccacc cggaataccg cgtgcagggc
    17161 gatctcagca cgccagaatt acaggaaacg ctcacgccgg tttatccaac aacggaaggc
    17221 gtaaagcagg ccacgctgcg taaattaacc gaccaggcgc tggatctgct cgacacctgc
    17281 gccattgaag aactcctgcc gccggaactg tcacaaggaa tgatgacgct accggaagcg
    17341 ttgcgcactt tgcaccgccc gccaccgacg ctacagctta gcgatctgga aaccgggcag
    17401 catccggcgc aacgtcgtct gattctggaa gaactgctgg cgcacaacct cagcatgtta
    17461 gccttacgtg ccggagcaca gcgttttcat gcccagccgc tgagcgccaa tgacacgctg
    17521 aaaaataaac tcctcgccgc cttaccgttc aagccaacgg gcgcacaggc acgcgtagtg
    17581 gcggagatcg agcatgatat ggcgctggat gtgccgatga tgcgcctggt acagggcgat
    17641 gtaggttccg gtaaaacgct ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc
    17701 aaacaggtgg cactgatggc accaaccgaa ttactcgccg agcagcacgc caataacttc
    17761 cgcaactggt ttgcaccgct cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggc
    17821 aaagcgcgac tgtctcagca ggaagccatc gccagcggtc aggtgcagat gattgtcggt
    17881 acacacgcca tcttccagga acaggtgcag tttaacggcc tggcgctggt gattatcgac
    17941 gaacagcatc gttttggcgt gcatcagcgt ctggcattgt gggagaaagg ccagcagcag
    18001 ggcttccatc cgcatcagtt gatcatgacc gccacgccga tcccccgcac gctggcaatg
    18061 acggcgtatg ccgatctcga tacctcggtg atagatgagc tgccgccagg ccgcacgcca
    18121 gtgactacgg tcgctattcc tgatacccgc cgtaccgaca tcattgaccg cgtgcgccac
    18181 gcctgcataa ctgaaggtcg tcaggcgtac tgggtttgta cgttgattga agagtcagag
    18241 ttactggaag cgcaggcggc ggaagctacc tgggaagagt tgaaactggc gctaccagaa
    18301 ttgaatgttg gcctggtaca cgggcggatg aaaccagccg agaaacaggc ggtgatggcg
    18361 tcgtttaaac aaggtgagct acatctgctg gttgccacta ccgttattga agtcggtgtt
    18421 gatgtgccta acgccagcct gatgattatc gaaaaccctg agcgtctggg tctggcgcag
    18481 ttacaccagc tgcgcgggcg cgtaggtcgt ggcgcggtgg cttctcactg cgtgctgctc
    18541 tacaaaacgc cgctttctaa aacggcgcaa attcgcctgc aagtgctgcg cgacagtaac
    18601 gacggttttg tgattgcgca aaaagatctg gagattcgcg gccctggcga attgttaggc
    18661 acgcgtcaga cgggtaatgc tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg
    18721 atcccggaag ttcagcgcct ggcgcgacat attcacgaac gttacccaca acaggcaaaa
    18781 gccttgatag aacgctggat gccggagaca gaacgttact cgaatgcgta aaaggcggcg
    18841 gtgagaagac cgccgcttca tcggttaacc ggcaaaaatc ggcaacatta aatacagctt
    18901 aatcaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    18961 cgccatgtgc gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgccgttgg
    19021 cgttgcaccg aggccaaaac cacagtgacc cgcagccagc actgccgcat cgtagttttt
    19081 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    19141 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa
    19201 cgccatcgcc aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa
    19261 gacgcggtaa aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac
    19321 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    19381 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat
    19441 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    19501 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    19561 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    19621 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    19681 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    19741 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    19801 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    19861 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    19921 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    19981 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    20041 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    20101 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    20161 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    20221 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    20281 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    20341 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgctcagact
    20401 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    20461 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcactccagc gctattaatc
    20521 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    20581 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    20641 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    20701 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    20761 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    20821 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg
    20881 ctgacctcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    20941 aatctgctgc tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac
    21001 ccttacttac gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg
    21061 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    21121 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    21181 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    21241 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    21301 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    21361 aacggagtga tccagttgac tggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    21421 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    21481 ccggttctgg gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt
    21541 atcgtttctc gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    21601 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    21661 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    21721 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    21781 cctctttcac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    21841 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    21901 gctctgttag tggtgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    21961 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat
    22021 caccgttttt ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggt
    22081 cagcccgcgc ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg ccaactgacc
    22141 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    22201 accgcgccgc taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc
    22261 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca
    22321 ccagaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    22381 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    22441 gttacgctga ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag
    22501 cgtaacgccg acggcagctg gcaagtggaa aatctgcgca tggcggatat ccgtctacaa
    22561 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    22621 ggtcgcttgg aagtcatcga tgctcgtctg caaggtccgg actgggcggt gaccgacctc
    22681 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggcaaa
    22741 ctgtcgatga acgccagcga gttcatttat ggttcgctgc atttatttga cccgattata
    22801 aacgcggaat tttccccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    22861 ggtatggtca gaacgtcagg gaactggctg cgtgacggta aaacgttgat ccttgatgat
    22921 gcggcaattg ccgggctgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    22981 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    23041 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    23101 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    23161 gccgccgccg cgacattcaa tcgtgttgat gttcgtcgcc cgtcgctggc gctgaccgcc
    23221 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    23281 gccaccgcaa gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc
    23341 ggtgtgccgg tgaatatttt gcaacaatgg ggatggcctg aattaccgtt gactggcgac
    23401 ggcaatattc agcttaccgc cagtggagat attcaggcca atgccccgct gaaacctacg
    23461 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    23521 gcgggcgtcg tttccagcgg tgaagttaca tcgacggagc cggtgcggta aacatcaatc
    23581 tcgccgggta cgactcagac gtacccggca ttccgtcaat agattacaac gtaattgtca
    23641 gcgcattccc ttgaggcgtc accaccagcc cctgctcact ttcagcctgc gaaccgcctt
    23701 gcagaccatt tactttcaca acattgcgca agcacagcgt ccagttcttc gcctcgcccg
    23761 caccagtcac agtaatcgtg ttgccagtac gtgctgcttt taaagtaaag atcaccgatc
    23821 cgtcagcagc aggcacttca cagacggctt catgcccgtc ttgcagatta aagaggtgga
    23881 atgccgtgcc ttcgtgccac acgtaatcgg gacgttgatc gttgttgccc agcgccagta
    23941 gagtgttatc acgcacataa acgggcagac tcaggaagcc gtgctgctgt ttatgccagc
    24001 gactaccgtc gagttcatcg ttgtgccaca ggtgtgtcca gcgaccttcc ggcaggtaga
    24061 actgcacatc gcccgcttca gtgaacaccg gcgcaaccat cacgttgtcg cctaacatgt
    24121 attgacggtc aaggtaatca caagccggat cgtccgggaa ctccatcatc atggcccgca
    24181 tcatcggcgt accccgcgcg ttcgcacgcg cagcttcacg atacagatac ggcatcatgc
    24241 ggcatttcag ttgcgtgaag aagcgcacca catcacagga ctcatcatcg taggcccacg
    24301 gcacacgata agatttgcta ccgtgtaaac ggctatggct ggagagcaaa ccaaacgcgc
    24361 accagcgttt gtaaacgtgc gccggagcgg tattttcaaa gccgccgata tcgtggctcc
    24421 agaagccaaa acctgaaagg ccaatagaca aaccaccgcg caggctttcc gccattgatt
    24481 cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa tttctgcgca ccgacggagg
    24541 ccgagcgggc aaacaagaca gcttcttcct caccaacggt gtccttgagc acgttccaca
    24601 ccagttcgtt gtagatgtac gcataatggt tatgcatttt ctgcggatcg gaaccgtcaa
    24661 accactgaac atcaattggg atacgttcgc caaagtcggt cttaaagcaa tcaacgccca
    24721 tcgcgaccag acctttcagt ttgtcggcgt accatttgca ggcatccgga ttggtaaagt
    24781 cataaatcgc cagacctggc tgccatttat cccactgcca tagcgaaccg tccgggcgtt
    24841 tgagtaaata gcctttctct tgtaactctt taaagacggg ggatttttga ccgatatagg
    24901 ggttaatcca gacgcagatt ttcagccctt tcgctttcag gcggcggatc atcccttccg
    24961 ggtcagggaa agtcagcggg tcccactcaa aatcgcacca ctggaaggct ttcatccaga
    25021 aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc cgccatacca tcgataaagc
    25081 tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt ggttagccac aggccgaagg
    25141 accacggggg cggcagcgcc ggacgaccag taaagcgggt ataacgatca agtaccgctt
    25201 tcggcgtcgg gccgtcgata acaaagtatt cgagatattc actctcaacg ctgaactgca
    25261 ctttggagac tttctccgat cccacttcaa aagagacaca ctggggatga ttgaccagta
    25321 ccccataacc acggttagtc atgtagaacg ggatattttt atacgcctgt tcagtacttg
    25381 tgccgccgtc ccggttccag gtctctaccg tctggccatt gcgcaccagg gcagtaaagc
    25441 gctctcccag accgtaaact gtttcgccaa cgccaagatc aagccgctca aacatataat
    25501 tgcgttgatt attcgtgtcc tgcacgtagc cattattttt cacctgacta ccggtaatac
    25561 gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc tttgctgaca cgcgcgctta
    25621 agttgccact tttaaactca gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct
    25681 gcaaaatatt gagcggataa tgaggaccgt tattcagcgc cccctgaaaa tgctcaatcc
    25741 gcacaccgac aataccttcc tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa
    25801 gctgccaggt acgttcacgc acatcacggg gggcagcata gaccaccatt tcattatcct
    25861 gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc
    25921 agtttccatc gctaattttc attgccgttc cttagttctg cacttcttga gaggtgaatt
    25981 gttgctggca acggcgttta ccctgagcca gctgttccat aacggttttc agattgtgcg
    26041 tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag caaataacag atcgccggaa
    26101 caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct gttctgcgct ttttctgccg
    26161 catcatatcc gccataagcc agcatccagc cgataagagc gccgccgaag gccaacccca
    26221 gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt caggcgttta ccattgcacc
    26281 actcgccgta gtcgacggta tcgaacatca ttacccactg gataggtgtc accagttgat
    26341 gcaacacacc aatcacgaag atgaagacaa acatagtgat gctggcctgc atgggaacaa
    26401 agaacatcgc gaggctaatc actgccagca gggcgttcgt ccaccagaag atagtgactt
    26461 tacatttcca gtcggtcaga ggttttgcca gtgcggaacc aatcaggtta cccacgcaat
    26521 aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa aatccatgtg acgtaataca
    26581 tcatcgcccc accgcgtacg cacaccgcca ggatattgaa aatggttagt aaaccgacaa
    26641 tccgccactg gtcgttttgc cagatatcac gtaaatcttc ccgcatagac gttgttgtag
    26701 atggtgcttc aacgcgttct ttagtggtga agaaacaaaa tgccagcatc atgaatgcca
    26761 ccacggaaag gaccacgata ccgccctgga aaccgagtgg tttattatca ccgccaatta
    26821 aattaaccag tggcatcatc agaacagtag aaagcatgcc tcccgcggtc gccagcacaa
    26881 aacgccagga ttgcagcgag atacgctgag tcgggtcatt ggtgattaca ccacccaatg
    26941 cgcagtaagg gatattgacg acggtataaa gtaaggtaag ttagggtgta agtaattgct
    27001 gcatagatca ttttgccgtt catactgaga tctggcgtgc tataggccag tacacagacg
    27061 atcccgaatg gcagtgcgcc aaacagtacc cacggacgaa atttacccca gcgagagcgc
    27121 gttcgatcgg ccaacaaccc catgcaagga tcggaaatcg catccagtgc acgagcgacc
    27181 aaaaacatgg ttccgacaaa tccggcagga atgccaaaaa tatcggtata aaagaacatc
    27241 atatataaca ttacgttatc gaaaataatg tggctggcgg cgtctcccat gccataacca
    27301 attttctctt taacggacaa cacttcactc ctcatctgct tttccttgat gctatagggg
    27361 tgctgagacc gataacgcat tttgtaaacc atccctgtga gagcgaatat tccgatatct
    27421 ggttattaat ttatgattct tgttttatgt gatcgtggta gcgttaattc cgctcatata
    27481 tcattgtaaa atatgggttt tatataaatt ataatgcttt cgtgataata cgctgcgttt
    27541 attaggcgga aaaaactgat ctgggggatg tagaaactca aggaagtagc tataatgcgc
    27601 cccgcctcca tgtagcaatc gaggcgcgga agatcgtcgt ctccggtgag gcggctggac
    27661 ttcaaatcca gttggggccg ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc
    27721 cagtttacct cttctgaact ctcccaatgt caataaatct cttctaatcc cttgtatatc
    27781 agtatttatt tgtatcctga ggtcaaccta cttcaccccg tatcaagttt cttttggggg
    27841 tactttaggg ggtgttattt cctattgaaa tacaagcacc cccataatag gaaaaatgtg
    27901 atacataaca acggctttta acaatatctt tgttatacaa ggggtgtatt gggggtatca
    27961 ggttagttac tggcagaaat ccccctcagg aacatgaatt catcaaaaag ccggaggatg
    28021 tcatggcatt aaccgatatc aaagtgaaaa ccgcaaaacc caaagacaaa ccctataagc
    28081 ttgctgatgg cggcggtatg tatctactga ttaacactaa tggttctaaa tactggcgca
    28141 tgaagtaccg ctttgctggt aaagaaaaga tgctttctat tggcgtatac cccgatgtaa
    28201 cgctagccga tgctcgcgag aaacgcagtg aagccaggaa actcctcgct gctggaggcg
    28261 atccaaggga ggcaaaaaag gaagaaaaaa tcgcgcagca aatgagtctg aaaaatacgt
    28321 tcgaggctat tgctcgcgaa tggcaccagt taaaagctga tcggtggtca ttacgctatc
    28381 gtgatgaaat cattgatacc tttgaaaaag acatttttcc ctacattggc aagcgcccta
    28441 ttgccgaaat caaaccaatg aagttactgg aagcactgcg taaaatggaa aaacgtggag
    28501 ctctggagaa aatgcgcaag gtccgccagc gctgtggtga ggtgttccgc tatgcaattg
    28561 ttactggccg ggcagactat aacccagccc ccgatcttgc cagtgcttta gctacaccga
    28621 aaaaagtaca ttttcccttt cttactgcta atgaacttcc ccacttcctc aacgatctgg
    28681 cgggttatac cggaagtata atcactaaga cagctactca gatcattatg ttaaccggcg
    28741 tacgaacaca agaactgcgt tttgcacgct gggaagatat cgattttgag acaaaattgt
    28801 gggagatccc ggcagaagta atgaagatga agcgacctca tattgtgccg ctctctgagc
    28861 aggtcattat gctatttaag cagcttgaac caatctcaaa acatcatccc ctagtcttta
    28921 tcggtagaaa tgatccccgc aaaccaatta gtaaggaaag cattaaccaa gtaattgaat
    28981 tacttggata taagggaaga ctcacagggc acggtttcag acacaccatg agtacaattc
    29041 tgcatgaaca aggctttaat tccgcctgga ttgaaatgca gttagctcat gtggataaaa
    29101 attccatcag gggtacctat aatcatgccc tgtatctcga tggccgccgt gaaatgatgc
    29161 aatggtacgc tgattacatt gattcactct ccagccggga aagttaagtc agaaatactt
    29221 ttccctgatg ttttcctcaa ttaagtattt tatcctctgg cctacaaggc tggaggattt
    29281 ttgttattat attatttttt tgcgtcttca aacttattac aaaaacataa ttaaaaacaa
    29341 attgacattt ttaaattggc aattacaaaa tacagccttg cagttatttc gataccttta
    29401 gcaaaggaat agttgattca gatataattt agcatttcaa tcatctgaca tcattataaa
    29461 aaatgagatt cacataaaga taaaattatt gttctcaagg aaagcatgaa ggggcttcgt
    29521 aagcaatatt cattcggtgc caagacagga cttgatacac agaatctcaa cttttatgct
    29581 ctcagactat atcaccacat gttttacagg ctcagaaaat gaacgataga ttatgctttg
    29641 aagtgcacga taacaagggt tatttcgtat ttccggatac ctggtttggc ccattgctgg
    29701 gggagtttga agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca
    29761 tcaacaaact ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc
    29821 tggcatatgc atttctggaa cagaatgccc cgcgtaaggc actgaacgcc gctcttaaag
    29881 gactggcggc aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc
    29941 atcccgaaaa ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact
    30001 tacagcgcca tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaaa
    30061 ataatcaggg ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc
    30121 gagccttcag tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg
    30181 gtctcctgca ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct
    30241 ttgccacaaa cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg
    30301 ctgtgtggca tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact
    30361 caccattatg gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt
    30421 cctccgtgct gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag
    30481 atgatttcga aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg
    30541 acaagacact ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct
    30601 ggccctggat attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt
    30661 agatattgca ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg
    30721 tgttccttcc ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga
    30781 tagcgttgta aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata
    30841 ctttatcgtc aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg
    30901 taatcccctc ctctggcgtt ttcggttgaa ctggtatccc cgtccaacac aggcagccat
    30961 tataaacagt aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac gtactgttga
    31021 atgtgcttgt gttctgaacc tcgtacaacc agtacatttg atatcgaatt ataaacaaaa
    31081 cattgcatcc aactttttta atatggacaa tagttgtcca tacggtggtt ttatattcga
    31141 taaatcaatg gatttcattg agtcacagga ctggtaatgg atgaaaatgc tttaaggttt
    31201 gcctcatact ggcgcaactc gcttgcagat gctgagtcag gaaagggctg ttttgaacgg
    31261 aaagacgccc aaaatttcac tcactggcat gggatagcgg cgggacgtct tgacgaagcg
    31321 attgtcaata aattttttaa gggagaaaaa gacgatgtcg aaactgtcaa tgtcatctta
    31381 cgcccaaaag tttatttccg gttactgcag catggtaagg accgttccgc aggcacgcct
    31441 gatattgtta caccgatagt gacgccagcc ttgctaagcc gtgagggttt tttatatccg
    31501 acgccagcga cctccattcc cagagacctg cttgaacctt tgccaaaagg agcattttcg
    31561 attggtgaga ttgggcagta tgacaaatac aagacgaccc atactgatcc tacccacgta
    31621 atgtggacac ggccctaagc gagattatgg ttttcaaatt gttccgggct gagaccgcca
    31681 caggcactgt gacgacgcca gcgattgtaa tcacactcga tataattaaa caccgttgcc
    31741 cgcattattt cccggctgct aaagcgttcc ccgtggatac attccacctt cagcgaatga
    31801 aagaagcttt ccacacaggc attgtcataa cagtagcctt tcgcactcat actgccacgc
    31861 aggttgtgtc gcttcagcat tgcctgataa tcccctgaac agtattgacc accacggtcc
    31921 gtatgaacaa tgacgctttc cgggcgtctt ctccgccaca acgccatttg cagtgcatca
    31981 caggccagtt gtgctgtcat tcgcggtgac atcgaccagc caatgacgga gcgtgaccac
    32041 aggtcgatga ctactgcgag atacaaccag ccctcatcgg tacgcaagta cgtgatgtca
    32101 cccgcccact tctggtttcg ggccactggc gctgaagtcc tgctccagca gattctccaa
    32161 tacgggcagg ccatgtgcac ggtagctgac cgggctgaac ttccggctgg ctttcgcccg
    32221 cagcccctga cgacgcaggc tggcggcaat ggttttaata ttgaactccg gcagttcgtc
    32281 agcaaggcgg ggagcaccgt atcgctgctt tgcctcaaag aatgccttat ggacagcggc
    32341 atcgcaggtg agccgaaact gttggcgcag gctcatctgg tgacgacgcc tgagccagac
    32401 ataccagccg ctgcgggcaa ctcgaagtac acgacacatc gctttgatgc tgaactctgc
    32461 ccgatgattt tcgataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc
    32521 ttttggagga tggacagttc ctcagcctgc tccgccagtt gtcgtttaag gcggacattt
    32581 tcagcggcca gttcgctttc gcgctctgac gaactcattt gttgctgctg tttactgcgc
    32641 caggcataaa gctgagattc atacaggctg agttcacggg ctgcggcggc cacaccgatg
    32701 cgttcagcga gtttcagggc ttcgttacga aattcaggcg tatgttgttt acggggcttc
    32761 ttgctgattg atactggttt tgtcatgagt cacctctggt tgagagttta ctcacttagt
    32821 cctgtgtcca ctattggtgg gtaagatcac cctccattaa aactaaaacg ggcgctttct
    32881 gatatatcat ctgtaagcgc agtaatacca ttagtagcac ctctcggcgg cgcattgatt
    32941 attgaatatt ataactggaa tgttatattt cttgttatga tgctgttcag tattataata
    33001 atgttattat caccaaagat aactcatgca aatgagaaag taatatgtca aaacagtgca
    33061 tcctttgtga ccaaagagtt tatctgcggc actatacttg tctcattaat actcagttct
    33121 ctattttgct acatatcaat atcaccatca attttcatgc atacttatgg cctgagtact
    33181 tttagttact caattatttt ctccttctca gttttatttt tcataatcgg aaatcaatta
    33241 tcaaagattg aaaaattatc caacctatgg tttttattac ccaatagcca attttatcgc
    33301 attacaaata aatggttcaa aaacaggatt tgccgcatct attacaggtt ttactcaaac
    33361 aataagcgct ggtacaatca gtttcctgtc agtaaccgta agcgacaacg gtgttggtcc
    33421 cggaactact ttaggtcttg gttcactaat aacttcatta ctgtcaatag ccgtaatcgc
    33481 cattgcagga aataataaaa agtaagtgta ctctgactgc gtatgtatct acttgaaccg
    33541 ccccgg
//
LOCUS       DAEBXD010000038        33544 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEBXD010000038 DAEBXD010000000
VERSION     DAEBXD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33544
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.038"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            529..666
                     /locus_tag="KJL94_001875"
     CDS             529..666
                     /locus_tag="KJL94_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410246.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="KJL94_001876"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="KJL94_001876"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBD4410247.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /gene="virB"
                     /locus_tag="KJL94_001877"
     CDS             complement(2992..3921)
                     /gene="virB"
                     /locus_tag="KJL94_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBD4410248.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="KJL94_001878"
     CDS             complement(4138..4374)
                     /locus_tag="KJL94_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD4410249.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="KJL94_001879"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="KJL94_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBD4410250.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="KJL94_001880"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="KJL94_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBD4410251.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLNILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLENLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="KJL94_001881"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="KJL94_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBD4410252.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="KJL94_001882"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="KJL94_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBD4410253.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="KJL94_001883"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="KJL94_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBD4410254.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="KJL94_001884"
     CDS             complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="KJL94_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBD4410255.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="KJL94_001885"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="KJL94_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBD4410256.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="KJL94_001886"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="KJL94_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBD4410257.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="KJL94_001887"
     CDS             13562..15178
                     /locus_tag="KJL94_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBD4410258.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="KJL94_001888"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="KJL94_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBD4410259.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="KJL94_001889"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="KJL94_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBD4410260.1"
                     /translation="MSRFVFILLCFTPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="KJL94_001890"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="KJL94_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBD4410261.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /gene="mxiH"
                     /locus_tag="KJL94_001891"
     CDS             17140..17391
                     /gene="mxiH"
                     /locus_tag="KJL94_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBD4410262.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="KJL94_001892"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="KJL94_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBD4410263.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /gene="mxiJ"
                     /locus_tag="KJL94_001893"
     CDS             17703..18428
                     /gene="mxiJ"
                     /locus_tag="KJL94_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBD4410264.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="KJL94_001894"
     CDS             18425..18952
                     /locus_tag="KJL94_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBD4410265.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /gene="mxiN"
                     /locus_tag="KJL94_001895"
     CDS             18894..19589
                     /gene="mxiN"
                     /locus_tag="KJL94_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBD4410266.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /gene="mxiM"
                     /locus_tag="KJL94_001896"
     CDS             19582..19989
                     /gene="mxiM"
                     /locus_tag="KJL94_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBD4410267.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="KJL94_001897"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="KJL94_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBD4410268.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="KJL94_001898"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="KJL94_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBD4410269.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="KJL94_001899"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="KJL94_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBD4410270.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="KJL94_001900"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="KJL94_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBD4410271.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="KJL94_001901"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="KJL94_001901"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBD4410272.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spa15"
                     /locus_tag="KJL94_001902"
                     /gene_synonym="spaK"
     CDS             26022..26423
                     /gene="spa15"
                     /locus_tag="KJL94_001902"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBD4410273.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26427..27719
                     /gene="spaL"
                     /locus_tag="KJL94_001903"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26427..27719
                     /gene="spaL"
                     /locus_tag="KJL94_001903"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBD4410274.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27812..28168
                     /gene="spa13"
                     /locus_tag="KJL94_001904"
                     /gene_synonym="spaM"
     CDS             27812..28168
                     /gene="spa13"
                     /locus_tag="KJL94_001904"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBD4410275.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="KJL94_001905"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="KJL94_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBD4410276.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGLFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spa33"
                     /locus_tag="KJL94_001906"
                     /gene_synonym="spaO"
     CDS             29009..29890
                     /gene="spa33"
                     /locus_tag="KJL94_001906"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBD4410277.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spa24"
                     /locus_tag="KJL94_001907"
                     /gene_synonym="spaP"
     CDS             29895..30545
                     /gene="spa24"
                     /locus_tag="KJL94_001907"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBD4410278.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="KJL94_001908"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="KJL94_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBD4410279.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spa29"
                     /locus_tag="KJL94_001909"
                     /gene_synonym="spaR"
     CDS             30820..31590
                     /gene="spa29"
                     /locus_tag="KJL94_001909"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBD4410280.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31597..32625
                     /gene="spa40"
                     /locus_tag="KJL94_001910"
                     /gene_synonym="spaS"
     CDS             31597..32625
                     /gene="spa40"
                     /locus_tag="KJL94_001910"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBD4410281.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="KJL94_001911"
     CDS             32638..32880
                     /locus_tag="KJL94_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410282.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10465 a         4881 c         6713 g        11485 t
ORIGIN      
        1 ttgttgacta cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg ctgtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atataatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtggg ttttttgttt atcaccatgt
     2641 aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgtagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttcttatat ttgcatctgt aacttttaat ttgttgttta ttctatgttc
     4681 tgttgtggac tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgccat ttccacctag attctctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatactga caacatatcc cccgtttttt tgagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagttcttcc aatgcctttt taagcgagtt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatattatt gatggagttt gcaattttag cccacagttc tctatgagat
     6901 atcatttgat ctccatcaag ctcggcttct ttcggagctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatattc gttcctggaa agaatattca atagttgagc ggatttattt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7141 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7381 ttgattggtt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtttcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7621 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7741 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8161 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct
     8401 gggttggttt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8641 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattttctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9661 tttttctttt ctggtgactc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtgt taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgctgg gacttccaag cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10021 tgtgcattag ttgtattaat attttggtta gcagtaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcttgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggaaa accagtggtt gtggtgctta cattatgcat aataattact
    10201 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttagcttt taagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aatactggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaacctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcggtga tatttaaaga cataaggtct cctttttgtc
    10681 tgctttactt taataagtat aagatttaat ataaaagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tatagcgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataagt
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgcgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcat acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgatctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caataatttt
    11881 tattgatttt tcgatgattt cttctttttt atctgttgct tcggttttta attcgtttaa
    11941 tacacgagtc atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttggtg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatttc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 atcgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt ctttttgcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgc ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact agtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaaaacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 acgctctgcg gtattcaaca ttaatttgat gtctctacct gtgaaagagt gttgaattga
    12961 gtttttagct gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 attgaggaaa agcaataagg atttttgatt ctctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta tcaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg aggatcatac tttattaact
    13261 ctccattact tggtgattgt ggggatgtaa aatgtatttg gtatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctcgttt catcatgaaa tcccacaaga taaagtgcct
    13441 gatgtatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagaa agaaataagg ggatcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga gctgaagttt tttgcgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac gattagatac
    13861 ctataaactt ggtgaagtta ataaaagaca tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaaacga aaataaaaca
    13981 aatatttact gattatttaa ataataagaa ttggggaccg gtcgacaaga atattagtca
    14041 tcatgggaaa aattatggtt ttcaattaac tcctgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 tgatcatatc gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
    14341 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
    14461 cgcattaaaa gggttaaact ctaaaagggg ggagccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attgtaacca aaaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
    15121 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
    15241 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgctta gctactgatg atgaaggcgg
    15421 aaatctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggatg tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt ttaccccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaatattc catcaagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tctgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tattctgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgatgat gtatggaaga gggtgggagg ctgttggagc ctataatgca
    15901 ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctatagacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gacaatttcc cagaaaacat tattcctgta acagatagta
    16201 aatcggatgg tataatttat ttgaccataa gcaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga gcaaattgat atcaattcac aatttaattc atttgaatat gacgggattt
    16321 cttttcacct taaaaatatg agggaggata aatcgagagg ccatatacta aacggaatgt
    16381 ataagaacca ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacactgga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcgtc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 caaacaaaga aataaataga attcaacaat atatcaatca acgattccca tttatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtgttttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgata aattgaaaaa tgctctgatt gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg ggggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaaat aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgaacataa aaatatatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccgaat
    17161 gatgattgga cattgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 ggtgaactaa cattggcact agataaatta gctaaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atccaataca
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagtct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaagt taaatcggaa tttttaacaa atgaagtaat atatttattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttggtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg tgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgcaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgcttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttcagctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaactg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagcccaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatatagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggtgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc gtcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaataat atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataaaagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaacgttaa
    23161 aagataacct tcctctagat cgggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatt attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gacgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga agtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agataaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtccctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataaatgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta attttgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tggggtctta taatcaatga gctatacaaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tgttgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acagataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtattaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttactga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttagaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagacaa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggctc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcagtc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgactcattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgcactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagaaca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 tcttttagaa aaaaatatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgacatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtacct gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc tcaaaaattt gatacaatat ctgatattgt ccgtttttct gattctggct
    30181 tgatggaata taagcaatac ttgaaaaagc atacagatct tgagttagca cgttttttcc
    30241 aaaggtctga agaagaaaat gctgatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaatcaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttgtt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtggggtatc ctgtctaagg ccttgattga acaatatatc aatattcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat tatctattgg
    30661 tttgttacag acagtgactc aactccaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt agctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggccttct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggtat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tcttaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggcgaacat
    31561 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgactgttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg gaggctgttt aaggatataa tattattttt cttggcattt
    32161 tctattcttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttattttaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgcactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacggagac aggtaaatta tggacgaaaa gaagcttaaa gcccttgcgg
    33421 ctgaactggc taaaggcctc aaaactgagg ccgacctcaa tcagttttcc cgtatgctga
    33481 cgaaatatcc cggctcttaa aacgatagtg gctcaggctt tcatttttca gcgttcccca
    33541 gaag
//
LOCUS       DAEBXD010000039        33200 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEBXD010000039 DAEBXD010000000
VERSION     DAEBXD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33200
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.039"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="KJL94_001912"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="KJL94_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBD4410283.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="KJL94_001913"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="KJL94_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="KJL94_001914"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="KJL94_001914"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBD4410284.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4475)
                     /gene="araH"
                     /locus_tag="KJL94_001915"
     CDS             complement(3489..4475)
                     /gene="araH"
                     /locus_tag="KJL94_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBD4410285.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /gene="araG"
                     /locus_tag="KJL94_001916"
     CDS             complement(4490..6004)
                     /gene="araG"
                     /locus_tag="KJL94_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBD4410286.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /gene="araF"
                     /locus_tag="KJL94_001917"
                     /pseudo
     CDS             complement(6074..7063)
                     /gene="araF"
                     /locus_tag="KJL94_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7860..8363
                     /locus_tag="KJL94_001918"
     CDS             7860..8363
                     /locus_tag="KJL94_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBD4410287.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /locus_tag="KJL94_001919"
     CDS             complement(8443..8694)
                     /locus_tag="KJL94_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBD4410288.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="KJL94_001920"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="KJL94_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBD4410289.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="KJL94_001921"
     CDS             9158..9481
                     /locus_tag="KJL94_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBD4410290.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="KJL94_001922"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="KJL94_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBD4410291.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="KJL94_001923"
     CDS             complement(10186..10425)
                     /locus_tag="KJL94_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBD4410292.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="KJL94_001924"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="KJL94_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBD4410293.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="KJL94_001925"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="KJL94_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBD4410294.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="KJL94_001926"
     tRNA            complement(12749..12835)
                     /locus_tag="KJL94_001926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="KJL94_001927"
     tRNA            complement(12848..12921)
                     /locus_tag="KJL94_001927"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="KJL94_001928"
     tRNA            complement(12976..13051)
                     /locus_tag="KJL94_001928"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="KJL94_001929"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="KJL94_001929"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBD4410295.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="KJL94_001930"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="KJL94_001930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBD4410296.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="KJL94_001931"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="KJL94_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBD4410297.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16589..16765
                     /locus_tag="KJL94_001932"
     CDS             16589..16765
                     /locus_tag="KJL94_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410298.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /locus_tag="KJL94_001933"
     CDS             16752..16976
                     /locus_tag="KJL94_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBD4410299.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="KJL94_001934"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="KJL94_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="KJL94_001935"
     CDS             complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="KJL94_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBD4410300.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="KJL94_001936"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="KJL94_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBD4410301.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="KJL94_001937"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="KJL94_001937"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBD4410302.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="KJL94_001938"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="KJL94_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4410303.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="KJL94_001939"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="KJL94_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBD4410304.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="KJL94_001940"
     CDS             complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="KJL94_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBD4410305.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="KJL94_001941"
     CDS             complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="KJL94_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBD4410306.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="KJL94_001942"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="KJL94_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBD4410307.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="KJL94_001943"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="KJL94_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBD4410308.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="KJL94_001944"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="KJL94_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBD4410309.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="KJL94_001945"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="KJL94_001945"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD4410310.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /locus_tag="KJL94_001946"
     CDS             complement(28474..28887)
                     /locus_tag="KJL94_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4410311.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="KJL94_001947"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="KJL94_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBD4410312.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="KJL94_001948"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="KJL94_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBD4410313.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="KJL94_001949"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="KJL94_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31326..32534)
                     /locus_tag="KJL94_001950"
                     /pseudo
     CDS             complement(31326..32534)
                     /locus_tag="KJL94_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32542..32971
                     /gene="tnpA"
                     /locus_tag="KJL94_001951"
                     /pseudo
     CDS             32542..32971
                     /gene="tnpA"
                     /locus_tag="KJL94_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         8335 a         8479 c         7837 g         8549 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcca ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggttgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    31381 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca
    31441 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    31501 cgattttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga
    31561 acagcaagca cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca
    31621 taccagccct gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc
    31681 ggctggctga ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg
    31741 actatcatta cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg
    31801 atatttgcga tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg
    31861 ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcttctt ctggtttttc
    31921 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg
    31981 ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
    32041 gcctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca
    32101 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg
    32161 ctgttttcct gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc
    32221 tttttgaatc aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg
    32281 tctttcagtg actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg
    32341 gcatttttcc actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg
    32401 gccttatgat tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct
    32461 gcgaagcgcc tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc
    32521 tgtagtcgtt tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc
    32581 ggaaggcact gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc
    32641 cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct
    32701 tattttgaag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tctgttaatt
    32761 aactatcccc ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc
    32821 gggtaggtta ctgcgacggg atcgaccaga tattgctgcc aggtattacc gcaaagatgt
    32881 tttgtggcgt cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
    32941 ccaatacatt gaacaacagc aaacacctgg tcagatgaaa aaacgagcct tatatccccg
    33001 ccctgaagga cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat
    33061 ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag
    33121 agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga
    33181 actaatatca agcggcgcta
//
LOCUS       DAEBXD010000040        32633 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEBXD010000040 DAEBXD010000000
VERSION     DAEBXD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32633
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.040"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..956)
                     /locus_tag="KJL94_001952"
     CDS             complement(<1..956)
                     /locus_tag="KJL94_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBD4410314.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="KJL94_001953"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="KJL94_001953"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBD4410315.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KJL94_001954"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KJL94_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KJL94_001955"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KJL94_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBD4410316.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="KJL94_001956"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="KJL94_001956"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBD4410317.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="KJL94_001957"
     CDS             complement(5018..6346)
                     /locus_tag="KJL94_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD4410318.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="KJL94_001958"
     CDS             complement(6354..8702)
                     /locus_tag="KJL94_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD4410319.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KJL94_001959"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KJL94_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBD4410320.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KJL94_001960"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KJL94_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBD4410321.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KJL94_001961"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KJL94_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBD4410322.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KJL94_001962"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KJL94_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBD4410323.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KJL94_001963"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KJL94_001963"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD4410324.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="KJL94_001964"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="KJL94_001964"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBD4410325.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="KJL94_001965"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="KJL94_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBD4410326.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KJL94_001966"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KJL94_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBD4410327.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19171
                     /locus_tag="KJL94_001967"
                     /pseudo
     CDS             18273..19171
                     /locus_tag="KJL94_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
     gene            19177..21609
                     /locus_tag="KJL94_001968"
     CDS             19177..21609
                     /locus_tag="KJL94_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBD4410328.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21755..22570
                     /gene="ybiV"
                     /locus_tag="KJL94_001969"
     CDS             21755..22570
                     /gene="ybiV"
                     /locus_tag="KJL94_001969"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBD4410329.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22722..23987
                     /locus_tag="KJL94_001970"
     CDS             22722..23987
                     /locus_tag="KJL94_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBD4410330.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24228..25820)
                     /locus_tag="KJL94_001971"
     CDS             complement(24228..25820)
                     /locus_tag="KJL94_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBD4410331.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26039..26959
                     /gene="ldtB"
                     /locus_tag="KJL94_001972"
     CDS             26039..26959
                     /gene="ldtB"
                     /locus_tag="KJL94_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD4410332.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27017..28135)
                     /locus_tag="KJL94_001973"
     CDS             complement(27017..28135)
                     /locus_tag="KJL94_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBD4410333.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28132..28599)
                     /gene="mntR"
                     /locus_tag="KJL94_001974"
     CDS             complement(28132..28599)
                     /gene="mntR"
                     /locus_tag="KJL94_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBD4410334.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28785..28913
                     /gene="mntS"
                     /locus_tag="KJL94_001975"
     CDS             28785..28913
                     /gene="mntS"
                     /locus_tag="KJL94_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBD4410335.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29185..30768
                     /gene="opgE"
                     /locus_tag="KJL94_001976"
     CDS             29185..30768
                     /gene="opgE"
                     /locus_tag="KJL94_001976"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD4410336.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30816..31331)
                     /gene="ompX"
                     /locus_tag="KJL94_001977"
     CDS             complement(30816..31331)
                     /gene="ompX"
                     /locus_tag="KJL94_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBD4410337.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31384..31449)
                     /locus_tag="KJL94_001978"
     CDS             complement(31384..31449)
                     /locus_tag="KJL94_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410338.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31684..32571
                     /gene="rhtA"
                     /locus_tag="KJL94_001979"
     CDS             31684..32571
                     /gene="rhtA"
                     /locus_tag="KJL94_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBD4410339.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8169 a         8699 c         7882 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatac catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 cccggaaaaa ccgcttgatg cgccagagct gctcgatttt gcccagcagt atgcctgcca
    19141 gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa actggacacg
    19201 ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa gccgccagtc
    19261 tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga taagccgatc
    19321 ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat ctggatcaaa
    19381 cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc gatcttcccg
    19441 gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg tcccggtgct
    19501 ggctttgcgg tgagcgaaga gaacaaacgt gttctgcatg aagtgtgccc atggtggcgc
    19561 ggtcagacgg tacaggatcg ctgctatggc atgtttaccg atgagcaaaa aggtctgctg
    19621 gcgaccggca tcattaaagc ggaaggcaat atgacctccg gcgatgcgca cctggcggtc
    19681 aatttcccgc tgctgctgga aaaagggctt gatggtctgc gtgagaaagt agcggaacgt
    19741 cgctcgcgca tcaatctgac ggtgctggaa gatttgcacg gcgaacagtt cctgaaagcg
    19801 attgatatcg tgctggtggc agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt
    19861 gcaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg aactgctggc gatggcagaa
    19921 aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct gcaactgtgt
    19981 tacttcatcc agttgatttt gcagattgaa tctaacggtc actcggtgtc gtttggtcgt
    20041 atggatcagt atctctaccc gtactatcgc cgcgacgttg aactgaacca gacgctggat
    20101 cgcgaacacg ccatcgaatt gctgcatagc tgctggttga aattgctgga agtgaacaaa
    20161 atccgctccg gctcccactc aaaagcctct gcgggaagtc cgctgtatca gaacgtcacc
    20221 attggcggac aaaatctggt tgatggtcaa ccaatggacg cggtgaatcc actctcttac
    20281 gcgatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac
    20341 catgcaggaa tgagtaacga tttcctcgac gcctgcgtac atgtgatccg ctgcggcttc
    20401 gggatgccgg cattcaacaa cgacgaaatc gtgatcccgg aatttattaa actcggtatt
    20461 gaaccgcagg acgcttatga ctacgcagcg attggttgta tagaaaccgc cgtcggtggc
    20521 aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt gatgctggcg
    20581 gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg
    20641 ttgtcggcag gtaacttcaa caacttcgat gaagtgatgg acgcgtggga tacgcaaatc
    20701 cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat gctggaagag
    20761 aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg agcgaaaagt
    20821 atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggtcgg tattgccaac
    20881 ctcggcaaca gcctggcggc agtgaagaaa ctggtgtttg aacagggcgc gattggtcag
    20941 caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga gcaactgcgt
    21001 cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgataccgt cgatacgctg
    21061 ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa cccacgctac
    21121 ggtcgtggtc cggttggcgg caactattac gcgggtacgt cgtctatctc cgctaacgta
    21181 ccgtttggcg cgcaaactat ggcaacgccg gacgggcgta aagcccacac cccgctggca
    21241 gaaggcgcta gtccggcctc tggtactgac catctcggcc ctactgcggt cattggctca
    21301 gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca gaaactgaat
    21361 ccggcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct gcgtaccttc
    21421 tttgaagtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga aacgctgctg
    21481 gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc gggttattcc
    21541 gccttcttca ccgcgctctc tccagacgct caggacgata tcatcgcccg tactgaacat
    21601 atgctgtaat ctcttcggcc cggctgcggc gtcgccgggc taaatcattc acatcatcaa
    21661 tttcatcctt actttcattc gaaatataat ttgtgctctg cgtcacattg ttattacact
    21721 gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga
    21781 tatggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt ttatggcgca
    21841 atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta atcagtatta
    21901 ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg
    21961 cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc gacatgaatc
    22021 gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct
    22081 gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca
    22141 ctaccatcgc ctgaaacctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt
    22201 ttcgctcaac ctgccggatg aacaaatccc gttagtgatc gacaaactac acatagcgct
    22261 ggatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga ttattcccgg
    22321 tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa
    22381 tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc
    22441 ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga
    22501 taataatcat gaaggcgcgc tgaatgtgat tcaggctgtg ctggataaca catccccttt
    22561 taacagctga cctctctacc ggagccatgc gctccggttt tcccttcttt ttacatcctg
    22621 tacatcacat caaatcatta aacttgcatt aacttattta taacttgtat tgtcacatgc
    22681 aaattagcat agtttcgaat gaaagttaat gcgaggtggt tatggcttct acttttacca
    22741 gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac gcgctgcggg
    22801 cgcagcttgg cgatgtccag cagatcttta atcagctaag cgatgacatt gccacgcgag
    22861 tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg
    22921 ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa cgtcgcggtt
    22981 gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat cagtcgatgc
    23041 tggactatct gaaccgcaac cgctttgacg aggtctacaa aggccccggc gataatttct
    23101 tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg caggcgcaaa
    23161 tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga
    23221 catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc taccctgacc
    23281 gtatccgccg ccttccgccc ggaacgacct ccaaaggtct tggagcgcat accgactccg
    23341 gggcgctgga acgctggctg cttccagcgt atcagcacgt tttcgccaac gtctttaatg
    23401 gcaatctggc gaaatacgat ccctggcatg cggcacatcg tacggaagtt gaagagtaca
    23461 cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccagggctgg actgcgctct
    23521 cggatatgct gcctggtcag gggttgctgc acgtcgtgcc cattcctgaa gccatggcgt
    23581 acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc
    23641 ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag gcgttaacca
    23701 gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc atccattccg
    23761 ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct gcggcaccga
    23821 tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat
    23881 cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc tttacgcttg
    23941 ccgacctcaa cattcacggt aagcgagcgt tgggcatgga tgtttgagag ttaatggaag
    24001 tgaattgatg tgggattaag aaaatcgatt cgacgttact cagattgctg acaagtgcgc
    24061 tttcttaatg cctgatgcgg cgttaacgcc ttatccggcc tacaaagtcc tgcagattca
    24121 atatattaca ggtactgcgt aggcctgata aacgtagcgc atcaggctat tttgcatttg
    24181 tcatcagccg tcgtcacccc gacagtatcg gggtgacgga taatcactta ctcgatccct
    24241 ttactacgca ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt
    24301 tccagaatgc gggtcgccag ggagcttacg aactcacggt cgtgtgaaac aaagatcagc
    24361 gtgccctgat acagttccag cgccatgttc agtgactcga tggattccat atccaggtgg
    24421 ttggtcggtt cgtccatgat cagaatgttc ggcttctgca tcattaactt accaaacagc
    24481 atccgccctt tttccccacc ggaaagcact ttagctggct ttttgatgtc gtcctggctg
    24541 aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt catcgccttc ctgcttccac
    24601 tgactcatcc attcgaacac ggtcagatca ttttcaaact catattcgtg gtcctgggcg
    24661 tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc cgctgtccgg ttgcagatcg
    24721 cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag taccgcaagt
    24781 ttttcaccga cctccagcag caggttgaga tttttaaaca gtggaccgtt atcaaaccct
    24841 ttggtcagac cttccacttc cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg
    24901 ataaacgggt tctgacggct ggaggctttc acctcttcca gtttgatttt atcaatctgg
    24961 cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa
    25021 gattgcaact cagcaatctg cgctttcttc ttggcgttat cggccagcag acgttcacgc
    25081 gcctgggtcg ccgccgtcat gtactcatcg tagttacccg gataaacgcg cagctcgccg
    25141 taatccagat ccgccatgtg ggtacagacc atgttaagga agtgacggtc gtgcgagatg
    25201 atgatcatgg tgctgtcacg ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg
    25261 tcgaggttgt tggtcggttc gtcgagcagg agaatatccg gctcagcaaa cagcgcctgc
    25321 gccagaagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc gtagtgctgt
    25381 tccactggaa ttcccacgcc aagcaacagt tcaccggcgc gagcttccgc agagtaaccg
    25441 tccatttcgc cgtatttcac ttccagatcg gccactttat agccgtcttc ttcactcatt
    25501 tccggcaaag catagatgcg gtcgcgctcc tgcttcactt cccacaactc tttatgcccc
    25561 atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg acgcagctta
    25621 ccaatgcgct cgttgggatc gagggaaacg ttacccagcg tcggctcaag gtcgccgccg
    25681 agaatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc gtaacggttg
    25741 ccgccgccga atttgacgga aatgttttca aacaacggct tactgccgaa ctgcatggtg
    25801 acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat
    25861 gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa gccaaaggca
    25921 aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact tataatgcgc
    25981 ttccaatact ctaatattct caacccaatg gcctgccagg cacaaaatct cgcttaacat
    26041 gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct tttgcagtac
    26101 cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg gtcagaatca
    26161 ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg cggagtacca
    26221 gatggggctt tccaatatga tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg
    26281 cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc atgaaggcat
    26341 cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca acaccgttat
    26401 cgtgctgccg atcggcattg gtcagttagg caaagatacg cctatcaact ggaccaccaa
    26461 agttgagcat aagaaagcag gcccgacctg gacgccgacc gccaaaatgc acgcagagta
    26521 ccgcgctgcg ggcgaaccgc taccggctgt cgttccggca ggtccggata acccgatggg
    26581 tctgtatgca ctctacatcg gtcgcctgta tgctatccat ggcaccaacg ccaacttcgg
    26641 tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct
    26701 gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg taaaagcgac
    26761 caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta ccaccgaagc
    26821 ccagtttgaa ggtcaggaaa tcgtgccaat taccctgacg aagagcgtgc agacagtgac
    26881 cggtcagcca gatgttgacc aggttgttct tgatgaagcg atcaaaaatc gttccgggat
    26941 gccggttcgc ctgaattaat cgtcaaaacg taaaacaaag gcggactcat aatccgcctt
    27001 ttttatttgc cagaccttac caggccggga tcataactaa caaaacgtat cccaccagcg
    27061 ccgcccacaa cagcatcggt attgaataga gatggaaacg ccaccagatg cggcgatcgt
    27121 tcgccatacg tagcgcaatc aaatttgcca gcgatcctgg taataaacca aagcccccca
    27181 cgtttaccgc ccatgccagt aataaagacg gaggtacata attgagcagc aaaatggtac
    27241 tcggcacgtt actgatcacc tgcgataaac cgactgccgt taaccataac ccgggttcag
    27301 ataaatgact cacgttaccc aacacgcctt gcaacgctgg aagctgggtc agtaaatgga
    27361 cgtcgataaa catcgccata aacaccagca gcaacgtcca gtccacactc aacaccacgc
    27421 gacgcgccag cagcacaaag cccgccgcca caatcaccag tccccacagc tcttgtttga
    27481 actccagcgc cgtcagaaag acgatataca gccccagaca attccacacc agccgcggtt
    27541 tccactccgg tgtttgcacc cccgtatggt attgcagcgc ctttccaggg aaacaacacc
    27601 agcacaacag caggagtgtc agcatcattg cgccagccat cggtgccatt tgggcaataa
    27661 atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg ttgccaattg
    27721 gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa aataatcagc cgattaaccg
    27781 ggatctcaca cagtcttttt agcgtgatag tcagcggaac aacaataaac agcgcgacat
    27841 cgttggtcag aaaggtagaa agcagcgccg ccgccagcac cataaacatc gccagccgac
    27901 gctccgtagc aaagcggcgc accattttgc gccccagcac atcaaaataa ccgcttaact
    27961 ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag
    28021 cagcaggcca ggatttcggt gcaaagggca cgaaaaagct taatccgata ccaacaagaa
    28081 ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc
    28141 accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc
    28201 catgccttcc gcgtcgcgac gggcgatttc cggactgacg cccaacacca acaagaaatt
    28261 ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa
    28321 aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagcc gcttaagcat
    28381 tttagccacc gtcggttgcg aaactcccag acgagcagcc atgtccacct gacgagcttc
    28441 ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg
    28501 atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca ctaactgcgt
    28561 cacttttttt gctattggcg tacctgcgcg acgactcatt gtgcttcctc attatggtga
    28621 cgcatacata gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa
    28681 aaattaacgt tttagcaata gctatataat atagcctgtg ctatatctgt atgtaatgca
    28741 atcatccctc aaggatcgac gggattagca agtcaggagg tcttatgaat gagttcaaga
    28801 ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatgctg ctctctatgt
    28861 tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc ttcagataaa tagcggcgtc
    28921 gcggtacgcc gcttcactcc tgcttttatg caggcataac gcgttttggt ctgaaaaaaa
    28981 ccactttttg tcggatttgc aatccccttc gcaaaagatt tgttcgtcag tagttgacct
    29041 gaacggcggc tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga
    29101 cccctttccc ctcttcacgc actcttgcag gtattgaccc ttgacgccag ggtaagcaca
    29161 tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct tgttacccgt
    29221 agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt aattaacctc
    29281 ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat tttgcttttg
    29341 ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtgtcagttc gctggtggcg
    29401 gcttgttatt tcccttttgc tcaggcctac ggcgcgccta atttcaatac attgctggca
    29461 ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc gtggtacagc
    29521 tacctggtcg gcttatttat ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag
    29581 aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc tggtattcag tgtggcgaca
    29641 ttttttgttg ctcccgtgca aaacctggcc tggggtggcg tatttaaact gaaagatact
    29701 ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga ggtgattgaa
    29761 gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt cactgccgtt
    29821 aagccgaagt atcagaccta tgtggtagtg atcggtgaaa gcgcgcgtcg cgatgccctc
    29881 ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa cggtttgata
    29941 tttgctgact acattgctgc cagtggctcc acgcagaaat cgcttggctt aacgctcaat
    30001 cgcgtagtcg atggcaaacc acagtttcag gataactttg tcaccctggc aaatcgcgcg
    30061 ggcttccaaa cctggtggtt ttccaaccag ggccaaatcg gcgaatacga tactgctatc
    30121 gccagcatcg ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa ttttgaagca
    30181 gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct ggcgcaagag
    30241 cactcgcaac cgcagctgat tgttctgcat ctgatgggct cgcatccgca ggcctgcgac
    30301 aggacacaag gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg ctatctctat
    30361 accatgacgc aaacggacga tttactgcgc aagctgtacg atcagttacg caacagcggc
    30421 agcagcttct ctctggttta cttttctgac cacggtctgg cctttaaaga gcgcggcaaa
    30481 gacgtgcaat accttgccca tgatgataaa tatcagcaaa atttccaggt gccttttatg
    30541 gtcatttcca gcggcgataa agcgcatcgg gtgattaaag cccgccgctc agccaatgac
    30601 ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa tatcaaatac
    30661 ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca gttacagaag
    30721 gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc
    30781 gccccgagag gcggattttt atatcaccaa agtgattaga agcggtaacc aacaccggca
    30841 atccaagtgc ctacgtcaac gctacgaata cggctctgct cgtaagagaa gtccagagca
    30901 acgttttcca tcgggttgaa ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg
    30961 tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac cataacccac acccactaca
    31021 ccgtagatgc ttgcccagtc gttaatgcgg taagccggac cagcagtgat gccgtagtac
    31081 tggtttttgt tgtagtcacc agagcttgca gtacggcttt tctcggtgta agtgaaagaa
    31141 ccgatcacac ccagcgggct gttgtcttct tcatagcggt atttcaggtt gaaaccgccc
    31201 attttgttca tttggccctg agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc
    31261 gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga aagacatgca
    31321 atttttttca taaccacctc aaatgtgatt caaataagtc ctaagtttta aatatatcaa
    31381 aaattaatgg gaaactcttc gcgatttgtg atgtctaacg ggccatttca tgtaacagaa
    31441 cgtttccata caccgctatc catctaaatt taaatcactt tttcagagaa ctgcgtaagt
    31501 attacgcatg ttttccctgt cattcatcca gattattcct aatcaccaga ctaatgattc
    31561 catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc
    31621 agcattgggt atatccagta cactccacgc tttacttaag tctagatatt tgtgggagaa
    31681 aggatgcctg gttcattacg taaaatgccg gtctggttac caatagtcat attgctcgtt
    31741 gccatggcgt ctattcaggg tggagcctcg ttagctaagt cactttttcc tctggtgagc
    31801 gcaccgggtg tcactgcgct gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt
    31861 aagccatggc gactgcgctt tgccaaagag caacggttac cgctgttgtt ttacggcgtt
    31921 tcgctgggtg ggatgaatta tcttttttat ctttctattc agacagtacc gctgggtatt
    31981 gcggtggcgc tggagttcac cggaccactg gcggtggcac tgttctcttc tcgtcgcccg
    32041 gtagatttcg tctgggttgt gctggcggtt cttggtctgt ggttcctgct accgctgggg
    32101 caagacgttt cccatgtcga tttaaccggc tgtgcgctgg cactgggggc cggggcttgt
    32161 tgggctattt acattttaag tgggcaacgc gcaggagcgg aacatggccc tgcgacggta
    32221 gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt
    32281 gaagcactct ggcactggtc ggttattcca ttgggtctgg ctgtcgctat tctctcgacc
    32341 gctctgcctt attcgctgga aatgattgcc ctcacccgtt tgccaacacg gacatttggt
    32401 acgctgatga gcatggaacc ggcgctggct gccgtttccg ggatgatttt cctcggagaa
    32461 acactgacac ccatacagct gctggcgctc ggcgctatca tcgccgcttc aatggggtct
    32521 acgctgacag tacgcaaaga gagcaaaata aaagaattag acattaatta aatttacatt
    32581 tctgtatggt tatgcataac catacagaat ttctcgctac ttttcctcta cac
//
LOCUS       DAEBXD010000041        32143 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEBXD010000041 DAEBXD010000000
VERSION     DAEBXD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32143
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.041"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="KJL94_001980"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="KJL94_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="KJL94_001981"
     CDS             1128..1235
                     /locus_tag="KJL94_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410340.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="KJL94_001982"
     CDS             complement(1288..2046)
                     /locus_tag="KJL94_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBD4410341.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="KJL94_001983"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="KJL94_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBD4410342.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="KJL94_001984"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="KJL94_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBD4410343.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="KJL94_001985"
     CDS             3759..4619
                     /locus_tag="KJL94_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBD4410344.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="KJL94_001986"
     CDS             complement(4660..5196)
                     /locus_tag="KJL94_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBD4410345.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFIGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="KJL94_001987"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="KJL94_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBD4410346.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="KJL94_001988"
     CDS             6174..6764
                     /locus_tag="KJL94_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4410347.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="KJL94_001989"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="KJL94_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..9107)
                     /locus_tag="KJL94_001990"
                     /pseudo
     CDS             complement(8291..9107)
                     /locus_tag="KJL94_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="KJL94_001991"
     CDS             complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="KJL94_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBD4410348.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9830..12091
                     /gene="katE"
                     /locus_tag="KJL94_001992"
     CDS             9830..12091
                     /gene="katE"
                     /locus_tag="KJL94_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBD4410349.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="KJL94_001993"
     CDS             complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="KJL94_001993"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBD4410350.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12909..14261)
                     /gene="celF"
                     /locus_tag="KJL94_001994"
                     /gene_synonym="chbF"
     CDS             complement(12909..14261)
                     /gene="celF"
                     /locus_tag="KJL94_001994"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBD4410351.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="KJL94_001995"
                     /pseudo
     CDS             complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="KJL94_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="KJL94_001996"
     CDS             complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="KJL94_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBD4410352.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="KJL94_001997"
     CDS             complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="KJL94_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBD4410353.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="KJL94_001998"
     CDS             complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="KJL94_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBD4410354.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="KJL94_001999"
     CDS             complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="KJL94_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBD4410355.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18218..19045
                     /gene="nadE"
                     /locus_tag="KJL94_002000"
     CDS             18218..19045
                     /gene="nadE"
                     /locus_tag="KJL94_002000"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBD4410356.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19275..20162
                     /gene="cho"
                     /locus_tag="KJL94_002001"
     CDS             19275..20162
                     /gene="cho"
                     /locus_tag="KJL94_002001"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBD4410357.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20122..20697)
                     /gene="ves"
                     /locus_tag="KJL94_002002"
     CDS             complement(20122..20697)
                     /gene="ves"
                     /locus_tag="KJL94_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBD4410358.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20900..21385)
                     /gene="spy"
                     /locus_tag="KJL94_002003"
     CDS             complement(20900..21385)
                     /gene="spy"
                     /locus_tag="KJL94_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBD4410359.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21715..22683)
                     /gene="astE"
                     /locus_tag="KJL94_002004"
     CDS             complement(21715..22683)
                     /gene="astE"
                     /locus_tag="KJL94_002004"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBD4410360.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22676..24009)
                     /gene="astB"
                     /locus_tag="KJL94_002005"
                     /pseudo
     CDS             complement(22676..24009)
                     /gene="astB"
                     /locus_tag="KJL94_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24006..25484)
                     /gene="astD"
                     /locus_tag="KJL94_002006"
     CDS             complement(24006..25484)
                     /gene="astD"
                     /locus_tag="KJL94_002006"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBD4410361.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25481..26515)
                     /gene="astA"
                     /locus_tag="KJL94_002007"
     CDS             complement(25481..26515)
                     /gene="astA"
                     /locus_tag="KJL94_002007"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBD4410362.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26512..27732)
                     /gene="astC"
                     /locus_tag="KJL94_002008"
                     /pseudo
     CDS             complement(26512..27732)
                     /gene="astC"
                     /locus_tag="KJL94_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28178..28984
                     /gene="xthA"
                     /locus_tag="KJL94_002009"
     CDS             28178..28984
                     /gene="xthA"
                     /locus_tag="KJL94_002009"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBD4410363.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29151..29859
                     /locus_tag="KJL94_002010"
                     /pseudo
     CDS             29151..29859
                     /locus_tag="KJL94_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29864..30541
                     /gene="ydjY"
                     /locus_tag="KJL94_002011"
     CDS             29864..30541
                     /gene="ydjY"
                     /locus_tag="KJL94_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBD4410364.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30556..31263
                     /locus_tag="KJL94_002012"
     CDS             30556..31263
                     /locus_tag="KJL94_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD4410365.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31263..31811
                     /locus_tag="KJL94_002013"
     CDS             31263..31811
                     /locus_tag="KJL94_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBD4410366.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31821..>32143
                     /locus_tag="KJL94_002014"
     CDS             31821..>32143
                     /locus_tag="KJL94_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD4410367.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8246 a         8242 c         7612 g         8043 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttccagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgataa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaatcatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     8401 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     8461 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     8521 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8581 atattatcga aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc
     8641 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8701 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8761 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
     8821 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8881 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8941 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     9001 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     9061 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     9121 ttattatctt atttaaatct aagtcattta caatataagt ttaagagcga cgccacagga
     9181 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     9241 taattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     9301 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     9361 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     9421 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     9481 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9541 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9601 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9661 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9721 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9781 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     9841 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9901 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9961 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
    10021 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
    10081 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
    10141 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
    10201 acgtattgtt catgcacgcg gctcagcagc tcacggttat ttccagccat ataaaagctt
    10261 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
    10321 taggttctct accgttcagg gtggagctgg ctctgccgat accgtgcgtg atatccgtgg
    10381 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
    10441 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
    10501 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10561 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10621 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10681 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10741 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10801 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10861 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10921 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10981 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    11041 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    11101 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    11161 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    11221 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    11281 ataccaagag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    11341 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    11401 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    11461 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    11521 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11581 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
    11641 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccatgaa
    11701 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11761 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11821 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11881 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11941 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    12001 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    12061 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    12121 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    12181 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    12241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    12301 ggatcacccc gatggcttgc gtcatccagc acttgcagga acagtgcctc gctaatctct
    12361 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattaaccgg taaatcacca
    12421 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    12481 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12541 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12601 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12661 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12721 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12781 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12841 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12901 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12961 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    13021 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    13081 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    13141 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgtgcccca gcttacaggt catttctacc
    13201 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    13261 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    13321 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    13381 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    13441 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    13501 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13561 ccagatactt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13621 ccatttacca gcacatcctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13681 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13741 atattacaca cgccgataaa acgtttaaat ccggtatgac gataaacggc ttcagtaacc
    13801 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13861 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13921 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13981 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    14041 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    14101 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    14161 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    14221 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    14281 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    14341 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatatc
    14401 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    14461 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgtttttttg
    14521 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
    14581 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
    14641 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactacggta
    14701 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
    14761 ccatctcaac aaactcttcc agccctgttt cgcggaaatt taatgaagaa atcactgttt
    14821 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcaaca aagcaataag
    14881 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
    14941 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
    15001 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
    15061 ctagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
    15121 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
    15181 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
    15241 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
    15301 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
    15361 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggctttcgct
    15421 gcggcaaaat cgccctgctt cgcctgtttc aatgcggcat acgccaggct acgcgcttgt
    15481 ccagagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
    15541 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcaagatta
    15601 cccgtaccgg catcgattaa aatttcaggg gttagcgata tcttcttcgc tctcttcttt
    15661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    15721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    15781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
    15841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    15901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    15961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    16021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    16081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    16141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
    16201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
    16261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    16321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
    16381 ggaaagaata ataaagccgg gaattaatgc cgagaaagaa cgcgatactg aagcaggtac
    16441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    16501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    16561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    16621 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    16681 tgccattaaa gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    16741 accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
    16801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    16861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    16921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    16981 atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata actctttgac
    17041 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    17101 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
    17161 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    17221 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    17281 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag agatagacat
    17341 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    17401 tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg gtaacttgcg
    17461 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    17521 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
    17581 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    17641 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
    17701 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    17761 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    17821 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    17881 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    17941 gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    18001 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    18061 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    18121 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    18181 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    18241 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    18301 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    18361 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    18421 ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    18481 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    18541 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
    18601 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    18661 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    18721 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
    18781 caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc tttataagaa
    18841 agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag tggcactcgg
    18901 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg aacaggtcgc
    18961 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaacgt
    19021 tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg aaaaagagta
    19081 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    19141 aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    19201 gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata gataaccagc
    19261 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    19321 caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc acccgaccag
    19381 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    19441 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    19501 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    19561 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    19621 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    19681 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    19741 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa
    19801 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    19861 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgagtgg
    19921 tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat
    19981 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    20041 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    20101 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    20161 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    20221 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    20281 aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    20341 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    20401 tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct gtaacggttt
    20461 taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt
    20521 cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    20581 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    20641 accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    20701 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatga ttatcggcaa
    20761 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    20821 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt
    20881 aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    20941 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga
    21001 tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    21061 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    21121 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    21181 tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    21241 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    21301 cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    21361 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    21421 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    21481 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    21541 aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt
    21601 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    21661 ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    21721 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa
    21781 cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt
    21841 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    21901 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc
    21961 gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag
    22021 atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacagacca gcgcgccaaa
    22081 atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagcgcctc
    22141 cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg
    22201 taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca
    22261 acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc
    22321 ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcggttcat
    22381 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    22441 caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat
    22501 ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg cgccttgcgg
    22561 tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt
    22621 gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc
    22681 cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    22741 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    22801 taagacgatc gcggtagtaa cgatccaccc agtcattgag cacattaaac aaggtatcgt
    22861 tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc ccccgcaacc
    22921 gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag acttttagtt
    22981 cactaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgct
    23041 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc
    23101 tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta
    23161 tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga acgcctgttg
    23221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgctctg
    23281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattaag
    23341 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    23401 atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac cgtaatgacc
    23461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
    23521 cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg attcagtgac
    23581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    23641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    23701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    23761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    23821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    23881 ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    23941 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    24001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
    24061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    24121 gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt actggcagca
    24181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    24241 aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt gttgttcgcc
    24301 atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaagcgg cccgaacacc
    24361 tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt cagcaacgat
    24421 gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    24481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    24541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagactagca
    24601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    24661 tgcccgactg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    24721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    24781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    24841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    24901 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    24961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    25021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    25081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    25141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    25201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    25261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
    25321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    25381 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    25441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    25501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    25561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
    25621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    25681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    25741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    25801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    25861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    25921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    25981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    26041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    26101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    26161 tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    26221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
    26281 ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    26341 catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgcc ctttcgatac
    26401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    26461 caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc atcatgatga
    26521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    26581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
    26641 catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg cgtaatcggc
    26701 attcagtaca cagccaatca acaaacctaa gccgcgaact tcactgaaca aaccatagcg
    26761 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    26821 catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg ccagcggatt
    26881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    26941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta acagatcggg
    27001 cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc cggtttgtac
    27061 ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa
    27121 cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa tcaccgcaca
    27181 ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    27241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    27301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct tatggctgcc
    27361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    27421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    27481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    27541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    27601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    27661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    27721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttgcatgtt aacaataagt
    27781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    27841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    27901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    27961 gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg gaaattactg
    28021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatca ttgacatcat
    28081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    28141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    28201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    28261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    28321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    28381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    28441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    28501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    28561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    28621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    28681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    28741 caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc aaacagcaga
    28801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    28861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    28921 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    28981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    29041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    29101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    29161 agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    29221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg
    29281 gattgttcgg ttatagcctc tatattctgt tattcatcaa tgccccctct ttctgttacc
    29341 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag gacacaactc
    29401 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    29461 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    29521 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    29581 aatattcaaa attacgctta cggattcacc accatcgcct tctggcctta tacccttatt
    29641 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    29701 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    29761 attctcatcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    29821 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    29881 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    29941 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    30001 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    30061 tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    30121 atcgctgttt atggcttatg cgcccccgaa agcattttat gaagccctga aagaggcagg
    30181 tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag
    30241 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    30301 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    30361 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    30421 caatgcaaca tacacttatg gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa
    30481 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    30541 aagccaagat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    30601 ctcatcatcc tgttattcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    30661 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    30721 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    30781 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    30841 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    30901 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    30961 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    31021 ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat acgttttcgc
    31081 tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta ttcctgggca
    31141 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg aactgtttat tctgtttgcc
    31201 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgac
    31261 tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc
    31321 atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta
    31381 tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc
    31441 cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg
    31501 tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caccgataaa ctgctggcag
    31561 tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg ctggagtacg
    31621 ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc cgactggctg
    31681 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
    31741 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    31801 aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    31861 tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    31921 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    31981 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg
    32041 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg ggcggctcgg
    32101 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaa
//
LOCUS       DAEBXD010000042        31961 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEBXD010000042 DAEBXD010000000
VERSION     DAEBXD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31961
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.042"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(78..968)
                     /gene="rarD"
                     /locus_tag="KJL94_002015"
     CDS             complement(78..968)
                     /gene="rarD"
                     /locus_tag="KJL94_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBD4410368.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1020..1487)
                     /locus_tag="KJL94_002016"
     CDS             complement(1020..1487)
                     /locus_tag="KJL94_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBD4410369.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1652..2521
                     /gene="pldA"
                     /locus_tag="KJL94_002017"
     CDS             1652..2521
                     /gene="pldA"
                     /locus_tag="KJL94_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBD4410370.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2654..4483
                     /gene="recQ"
                     /locus_tag="KJL94_002018"
     CDS             2654..4483
                     /gene="recQ"
                     /locus_tag="KJL94_002018"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBD4410371.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4547..5167
                     /gene="rhtC"
                     /locus_tag="KJL94_002019"
     CDS             4547..5167
                     /gene="rhtC"
                     /locus_tag="KJL94_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBD4410372.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5229..5849)
                     /gene="rhtB"
                     /locus_tag="KJL94_002020"
     CDS             complement(5229..5849)
                     /gene="rhtB"
                     /locus_tag="KJL94_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBD4410373.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5960..6982
                     /gene="pldB"
                     /locus_tag="KJL94_002021"
     CDS             5960..6982
                     /gene="pldB"
                     /locus_tag="KJL94_002021"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBD4410374.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGLWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6990..7790
                     /gene="yigL"
                     /locus_tag="KJL94_002022"
     CDS             6990..7790
                     /gene="yigL"
                     /locus_tag="KJL94_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBD4410375.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7866..8765
                     /gene="bioP"
                     /locus_tag="KJL94_002023"
     CDS             7866..8765
                     /gene="bioP"
                     /locus_tag="KJL94_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBD4410376.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8653..9606)
                     /gene="metR"
                     /locus_tag="KJL94_002024"
     CDS             complement(8653..9606)
                     /gene="metR"
                     /locus_tag="KJL94_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBD4410377.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9842..12103
                     /gene="metE"
                     /locus_tag="KJL94_002025"
     CDS             9842..12103
                     /gene="metE"
                     /locus_tag="KJL94_002025"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBD4410378.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12142..12957)
                     /locus_tag="KJL94_002026"
     CDS             complement(12142..12957)
                     /locus_tag="KJL94_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD4410379.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13219..13980
                     /gene="udp"
                     /locus_tag="KJL94_002027"
     CDS             13219..13980
                     /gene="udp"
                     /locus_tag="KJL94_002027"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBD4410380.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14121..15548
                     /gene="rmuC"
                     /locus_tag="KJL94_002028"
     CDS             14121..15548
                     /gene="rmuC"
                     /locus_tag="KJL94_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBD4410381.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15643..16398
                     /gene="ubiE"
                     /locus_tag="KJL94_002029"
     CDS             15643..16398
                     /gene="ubiE"
                     /locus_tag="KJL94_002029"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBD4410382.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16412..17017
                     /gene="ubiJ"
                     /locus_tag="KJL94_002030"
     CDS             16412..17017
                     /gene="ubiJ"
                     /locus_tag="KJL94_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBD4410383.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17014..18654
                     /gene="ubiB"
                     /locus_tag="KJL94_002031"
     CDS             17014..18654
                     /gene="ubiB"
                     /locus_tag="KJL94_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBD4410384.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18733..19002
                     /gene="tatA"
                     /locus_tag="KJL94_002032"
     CDS             18733..19002
                     /gene="tatA"
                     /locus_tag="KJL94_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBD4410385.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19006..19521
                     /gene="tatB"
                     /locus_tag="KJL94_002033"
     CDS             19006..19521
                     /gene="tatB"
                     /locus_tag="KJL94_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBD4410386.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19524..20300
                     /gene="tatC"
                     /locus_tag="KJL94_002034"
     CDS             19524..20300
                     /gene="tatC"
                     /locus_tag="KJL94_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBD4410387.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20342..21124
                     /gene="tatD"
                     /locus_tag="KJL94_002035"
     CDS             20342..21124
                     /gene="tatD"
                     /locus_tag="KJL94_002035"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBD4410388.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21121..21609)
                     /gene="rfaH"
                     /locus_tag="KJL94_002036"
     CDS             complement(21121..21609)
                     /gene="rfaH"
                     /locus_tag="KJL94_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBD4410389.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21776..23269
                     /gene="ubiD"
                     /locus_tag="KJL94_002037"
     CDS             21776..23269
                     /gene="ubiD"
                     /locus_tag="KJL94_002037"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBD4410390.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23315..24016
                     /gene="fre"
                     /locus_tag="KJL94_002038"
     CDS             23315..24016
                     /gene="fre"
                     /locus_tag="KJL94_002038"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBD4410391.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24208..25371)
                     /gene="fadA"
                     /locus_tag="KJL94_002039"
     CDS             complement(24208..25371)
                     /gene="fadA"
                     /locus_tag="KJL94_002039"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBD4410392.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25381..27570)
                     /gene="fadB"
                     /locus_tag="KJL94_002040"
     CDS             complement(25381..27570)
                     /gene="fadB"
                     /locus_tag="KJL94_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBD4410393.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27760..29091
                     /gene="pepQ"
                     /locus_tag="KJL94_002041"
     CDS             27760..29091
                     /gene="pepQ"
                     /locus_tag="KJL94_002041"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBD4410394.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29091..29705
                     /locus_tag="KJL94_002042"
     CDS             29091..29705
                     /locus_tag="KJL94_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBD4410395.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29744..31195
                     /gene="trkH"
                     /locus_tag="KJL94_002043"
     CDS             29744..31195
                     /gene="trkH"
                     /locus_tag="KJL94_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBD4410396.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31207..31752
                     /gene="hemG"
                     /locus_tag="KJL94_002044"
     CDS             31207..31752
                     /gene="hemG"
                     /locus_tag="KJL94_002044"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBD4410397.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7419 a         8142 c         8742 g         7658 t
ORIGIN      
        1 gtgagtctcc tgtcagcatg gcaatatgcc cactctcata caagagtcgg catatttttt
       61 cagaatatat ttatttttta tttggacgtt ctacgctgcg tataaatcgc gtccatcaca
      121 aaaattgcca gcgccaccca aataaaggtg aaagtcacca tcttatcggc acccggtttt
      181 tcaccataaa acgtcacagc cagcaggaac atcagcgtcg ggccaatgta ctgaaaaaag
      241 cctaacgttg agagacgcag gcgcgtggca gcggcagtaa aacacaacag cggtacggta
      301 gtgacaatac cggcggcgat cagcagtaaa ttcagcgaca tcgggttttg ccccatatgg
      361 ctggttgaac tgtcggcaat agcaaacagg taaatcgccg ccacgggcag cagccacatg
      421 gtttcaatta acatgccggt ttgtgcttct accgcaatct tcttgcgcac caggccgtag
      481 aaggcaaaac taaatgccag ccccagcgcg ataataggta gcgaaccaaa agtccacagc
      541 tggaccaaca cgccacatat cgccagaatc accgccagcc attgcatccg gcggaatcgc
      601 tcgccgagga aaatcatccc cagcacaatg ttcaccagcg ggttaataaa gtaaccaagg
      661 ctcgcttcca gcatatggtg attgttcacc gcccagataa acagtagcca gttgccacca
      721 atcagtacgg cagagactgc cagcataaaa attttctgtg gcgtctgaat cagcgttttt
      781 aaataggacc actggcggca aatgctcatc agcaccacca taaagaaaaa cgaccagatc
      841 acgcgatgcg tcaggatctc atcggcgggc acgtagtaaa tcagcttgaa gtatgctggc
      901 gcaatacccc aaataaaata agcggcaaga gcgagtaata cgccctgccg cgtttgtttt
      961 gcatccatcg ggaatactca tttttaattg gtaacagcag tttacctgct tttatgtctt
     1021 caacccacca tataggtggc ggtggcactg gcaatataaa gctgttcttc attgtgtaat
     1081 tcaacgcggg cgacggcgac tttattgcct gcacgcaaca ggctactggt agcagtaaaa
     1141 cgctcgcccc tgcctgggcg cagataatca acgcgaagat caatggtccc catccgcgat
     1201 agccgctggc gtagttcatc ttcactgatg gtttcgtggc gggttaaggt acttcccacg
     1261 cacaccaggc cggcggcgac atccagcgcc gacgcaatga ctccgccgtg caaaatgctt
     1321 tgcgcccagt tacccaccat catcggctga tttttaaagg ccagctgtgc gaactctttt
     1381 tcgtaacgct ccagttccat ccccaatgcg cggttaaatg gcatgtgata aacaaacatc
     1441 tcacccacta atttcagggc ttgttcagcg gtcagtacgg cagacatatc atccttacac
     1501 ttcattggtt aatgaaatgt tgattttatg cttctttgtt gttggtttct actttaggaa
     1561 gggataacta acggcaatgg agttaagtat gtaaaatagc ccgcagaaaa acattcacct
     1621 tatcaataat tcgttacgga gaacacgacc gatgcggact ctgcagggct ggttgttgcc
     1681 ggtgtttatg ttgcctatgg cagtatatgc acaagaggca acggtgaaag aggtgcatga
     1741 cgcgccagcg gtgcgtggca gtattatcgc caatatgctg caggagcatg acaatccgtt
     1801 cacgctctat ccttatgaca ccaactacct catctacacc caaaccagcg atctgaataa
     1861 agaagcgatt gccagttacg actgggcgga aaatgcgcgt aaggatgaag taaagtttca
     1921 gttgagcctg gcatttccgc tgtggcgtgg gattttaggc ccaaactcgg tgttgggtgc
     1981 gtcttatacg caaaaatcct ggtggcaact gtccaatagc gaagagtctt caccgtttcg
     2041 tgaaaccaac tacgaaccgc aattgttcct cggttttgcc accgattacc gttttgcagg
     2101 ttggacgctg cgcgatgtgg agatggggta taaccacgac tctaacgggc gttcagaccc
     2161 gacctcccgc agctggaacc gcctttatac tcgcctgatg gcagaaaacg gtaactggct
     2221 ggtagaagtg aagccgtggt atgtggtggg taatactgac gataacccgg atatcaccaa
     2281 atatatgggt tactaccagc ttaaaatcgg ctatcacctc ggtgatgcgg tgctcagtgc
     2341 gaaaggacag tacaactgga acaccggcta cggcggcgcg gagttaggct taagttaccc
     2401 gatcaccaaa catgtgcgcc tttatactca ggtttacagc ggctatggcg aatcgctcat
     2461 cgactataac ttcaaccaga cccgtgtcgg tgtaggggtt atgctaaacg acttgttttg
     2521 atgaacggtt gagtggttgg caaatctgga atccagcatc caggattacc ctctcagaga
     2581 ctaaaagcat tgcagtttct cgcgcaggcg ctgaaaatag cgcctgtttt tatttcaggc
     2641 aatcggggtg aatgtggcgc aggcggaagt gttgaatctg gagtccggag ctaaacaggt
     2701 tttacaagaa acctttggct accaacagtt tcgccccggc caggaagaaa ttatcgacac
     2761 tgtgctttcc ggtcgcgatt gcctggttgt tatgcccact ggtggcggaa aatccctttg
     2821 ctatcaaatt cctgccttat tgctaaacgg ccttaccgtg gttgtttcac cgctgatttc
     2881 gttgatgaaa gatcaggtgg atcaactaca ggccaacggc gtggcggcgg cgtgccttaa
     2941 ctcgacgcaa acccgcgaac agcaacttga agtgatgaca ggctgccgca ccgggcaaat
     3001 tcgcttactg tatatcgccc cggaacgcct gatgctggat aactttcttg agcatctggc
     3061 gcactggaat ccggtgttat tagccgtcga tgaagcgcac tgtatctccc aatggggcca
     3121 cgatttccgc ccggaatatg ccgcgctcgg tcagttgcgc cagcggttcc cgacgctgcc
     3181 gtttatggcg ctgaccgcca cagccgacga caccacgcgc caggatatcg tgcgcctgct
     3241 ggggctgaac gatccgctga ttcaaatcag cagttttgac cgtccgaata ttcgctacat
     3301 gctgatggag aaattcaaac cgctcgatca gttgatgcgc tacgtgcagg aacagcgcgg
     3361 taagtctggc attatctact gcaacagccg ggcgaaagta gaagacaccg ctgcgcgcct
     3421 gcaaagcagg ggtattagcg cggcggccta tcatgccggg ctggaaaata atgtccgcgc
     3481 cgacgtgcag gagaaattcc agcgcgatga tctgcaaatt gtggtggcga cggtggcgtt
     3541 cggcatgggc atcaataaac caaacgttcg cttcgtggtc cactttgata ttccgcgcaa
     3601 tatcgaatcc tattatcagg aaaccggtcg cgccgggcgt gatggcctgc ctgcggaagc
     3661 gatgctgttt tacgatccgg ctgatatggc gtggctgcgc cgttgtctgg aagagaagcc
     3721 gcaggggcag ttgcaggata tcgagcgcca caaactcaat gcgatgggcg cgtttgccga
     3781 agcgcaaact tgccgtcgtc tggtattgct gaactatttt ggcgaagggc gtcaggagcc
     3841 gtgcgggaac tgcgatatct gcctcgatcc gccgaaacag tacgacggtt caaccgatgc
     3901 tcagattgcc ctttccacca ttggtcgtgt gaatcagcgg tttgggatgg gttatgtggt
     3961 ggaagtgatt cgtggtgcta ataaccagcg tatccgcgac tatggtcatg acaaactgaa
     4021 agtctatggt atgggccgtg ataaaagcca tgaacattgg gtgagcgtga tccgccagct
     4081 gattcatctc ggcctggtga cgcaaaatat tgcccagcat tctgccctac aactgacaga
     4141 ggccgcgcgc ccggtgctgc gcggcgaatc ctctttgcaa cttgccgtgc cgcgtatcgt
     4201 ggcgctcaaa ccgaaagcga tgcagaaatc gttcggcggc aactatgatc gcaaactgtt
     4261 cgccaaatta cgcaaactgc gtaaatcgat tgccgatgaa agtaatgtcc cgccgtacgt
     4321 ggtgtttaac gacgcaacct tgattgagat ggctgaacag atgccgatca ccgccagcga
     4381 aatgctcagc gttaacggcg ttgggatgcg caagctggaa cgctttggta aaccgtttat
     4441 ggcgctgatt cgtgcgcatg tcgatggcga cgacgaagag tagtcagcag cataaaaaag
     4501 tgccagtatg aagactccgt aaacgtttcc cccgcgagtc aaatgtatgt tgatgttatt
     4561 tctcaccgtc gccatggtgc acattgtggc gcttatgagc cccggtcccg atttcttttt
     4621 tgtctctcag accgctgtca gtcgttcccg taaagaagcg atgatgggcg tgctgggcat
     4681 tacctgcggc gtaatggttt gggctgggat tgcgttgctt ggcctgcatt tgattatcga
     4741 aaaaatggcc tggctgcata cgctgattat ggtaggcggt ggcctgtatc tctgctggat
     4801 gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc ctgcaccaca
     4861 ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca atctcgccaa
     4921 tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg ataacgttgg
     4981 cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg cgtggtttac
     5041 cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac gtctggcgaa
     5101 gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt tgattatttc
     5161 gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc cgcatccgac
     5221 attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat aaacaacgag
     5281 ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag agcaatcctt
     5341 tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt ggtgacgccg
     5401 agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg aaatagcgcc
     5461 gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg ctggaacaaa
     5521 tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc ggcgcgccac
     5581 tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac ttcaaacgca
     5641 atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat atgaatcgcc
     5701 agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg gttgagcgag
     5761 gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat cgatgtcagc
     5821 aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta tttttgtgcc
     5881 acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat tttacgtggt
     5941 ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa gagaaaacgc
     6001 gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg atgaagcaga
     6061 gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac agcaccatga
     6121 ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg cggaactggc
     6181 ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg ggcagggacg
     6241 ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta atgattatgt
     6301 tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtctgtggc gtaaacgcta
     6361 tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac gccatccagg
     6421 tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc gtatgccgtc
     6481 atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc gtgatggcta
     6541 tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac tgacccacag
     6601 cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga ttcgcgtcgg
     6661 tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg tgttagccgg
     6721 tgcgggtgat gacgccacgc aaacgcttct cttacaagct gaagaggaac gcgtggtgga
     6781 taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc ctgtcgaagg
     6841 aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa aggacgcaat
     6901 gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact cacccagcgg
     6961 aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct gatttagatg
     7021 gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact ctgaagctgc
     7081 tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt gatgtggggc
     7141 aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt gcgcgcgttc
     7201 acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt gccagcgatc
     7261 tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc gacgacgaat
     7321 ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg gtgttcaaat
     7381 atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg tttttcacct
     7441 gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc tggggcgatc
     7501 gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc ggcgtgtcga
     7561 aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag gattgtattg
     7621 cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa ggctgcatta
     7681 tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt attggtacta
     7741 atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa tcgttcttta
     7801 tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc aaagagtttt
     7861 ccattgtggc gttacttatc atcaccacga ttctgtgggc cttctccttt agcttttatg
     7921 gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc gttggcttgg
     7981 cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa acggtcggcc
     8041 tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt ttccgcgctt
     8101 atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg ctctacatca
     8161 cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct tttagcgcct
     8221 tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac catttctgga
     8281 ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg gtgggttaca
     8341 aacgcctgat ggaaactcgc ccgatgccgc agtataacgc ctttgcgtgg ttctatcttg
     8401 gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag aaaatgccgc
     8461 aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc gggattggct
     8521 actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc attatgaata
     8581 atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa cagccgcact
     8641 ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg catcgtaagt
     8701 gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc gcgctgagcg
     8761 aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg cgtacagtcg
     8821 gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct caaaactctc
     8881 tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt gaatcaacaa
     8941 taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa gaaaatgccg
     9001 ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc tggcgagatc
     9061 ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca ccaggcgtac
     9121 ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat cggaggtcat
     9181 caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca cgcccgattt
     9241 aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg gtgtcagcca
     9301 ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg gttcattgca
     9361 ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca acaggatttc
     9421 tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga agccaaggcg
     9481 ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca acgttgccgc
     9541 agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt gttttacttc
     9601 gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata tacagtaacc
     9661 gccaattatg gatgtgtaaa catctggacg gctaaaatca ttcgtctttt aaatttatgg
     9721 tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg gcgatgaata
     9781 aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta gaggaagaaa
     9841 aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc gcgagctgaa
     9901 aaaagcgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc tgacagtagg
     9961 gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg acctgctgcc
    10021 ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc tgggtaatgt
    10081 tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt tccgtattgg
    10141 tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca aatggtttaa
    10201 caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca aactgacctg
    10261 gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg ttaagcctgt
    10321 gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac agtttgaccg
    10381 ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag aactggcgaa
    10441 acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac taccacaggc
    10501 gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga aactgctgct
    10561 gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc tgcctgttca
    10621 ggggctgcat gtggaccttg tacatggtaa agataacgtt gttgaactgc acaagcgcct
    10681 gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct ggcgcgccga
    10741 tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt tgtgggtggc
    10801 atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc gtcttgatgc
    10861 agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg cactgctgcg
    10921 cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc caattcaggc
    10981 gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg cggcgatcac
    11041 cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc agcgtgcgcg
    11101 ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa ccacggaaat
    11161 tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact accgcacggg
    11221 cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac tggatgtgct
    11281 ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc acctcgacgg
    11341 atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg tgaagccacc
    11401 gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg cgaagtatgc
    11461 gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga ccatactctg
    11521 ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga ttgcgctggc
    11581 gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc agattgacga
    11641 accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt atctccagtg
    11701 gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac aaatccacac
    11761 tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc tggacgcaga
    11821 cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt ttgaagagtt
    11881 tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa acgtaccgag
    11941 cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg cagagcgcct
    12001 gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc gcgcggcact
    12061 ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa ccgggtggta
    12121 ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct taaaccattc
    12181 cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc gataatcagc
    12241 gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat tagcagcccg
    12301 cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct gaccaccata
    12361 taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt tcggtgaatt
    12421 cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta gctgtggatt
    12481 atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga tcattaaacg
    12541 atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat cggccagcac
    12601 ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat cggcaaaatc
    12661 attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc cctccagcgc
    12721 cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct gcactacaat
    12781 gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag gcatgttatc
    12841 cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg tctgaacgat
    12901 ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg ttgccatggt
    12961 attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca tgcaacaaat
    13021 cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag tgatttatgt
    13081 gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg gtgatgagta
    13141 tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg actaaaccga
    13201 ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct cactaaaaac
    13261 gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt ggaaaagatc
    13321 gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac tacctggcgt
    13381 gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg cccgtctacc
    13441 tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg tatcggtaca
    13501 acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac ggcgtctgtc
    13561 cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt cgctgatttc
    13621 gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac tcacgttggc
    13681 gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac ttactctggt
    13741 cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg cgtaatgaac
    13801 tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct gcgtgccggt
    13861 atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc tgagacgatg
    13921 aaacaaaccg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg tctgctgtaa
    13981 ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc gtagcgcctc
    14041 gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg tgcgggtaag
    14101 ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt attgcgttgg
    14161 tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa aaagccgagc
    14221 aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa caaattaccc
    14281 aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc agcctgcaaa
    14341 gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg gaagccgcac
    14401 agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc ctcagtgagc
    14461 agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt gatgagcaaa
    14521 accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac ggtttccgcc
    14581 gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg acccacgaaa
    14641 ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac ctgacgcgcg
    14701 cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg acgcgggtgc
    14761 tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc atcgaaaatg
    14821 acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga aaagatgtgg
    14881 tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc gaagacgact
    14941 acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat atccgtttgc
    15001 tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac gtgctgatgt
    15061 ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag ctgatcaccg
    15121 aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg gcgttgcgta
    15181 ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa atcgccgatc
    15241 gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc gcgattggtc
    15301 aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc tcttcaggtc
    15361 gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa attaaacgcg
    15421 agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat cgacttcggt
    15481 cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat aatcagcagt
    15541 cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg acgcccaatc
    15601 tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga taagtcacaa
    15661 gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga tatggtcgcc
    15721 cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat gtcatttggt
    15781 attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg tgggcagacc
    15841 gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg cctggtcgga
    15901 gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat gggacgcgag
    15961 aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa cgctgaggcg
    16021 ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct gcgtaacgtc
    16081 accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg cggccgcctg
    16141 ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta tgatgcatac
    16201 tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga cagctaccgt
    16261 tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc catgatgcag
    16321 gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt ggcgctgcat
    16381 cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag tgacggcagg
    16441 aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa cagcccgctc
    16501 gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt cattgattct
    16561 ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg ctgactgcac
    16621 cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc ttaccgcact
    16681 gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa acttcgttgc
    16741 gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata ccggtgatat
    16801 cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc atcacggcat
    16861 taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta tggcacccgg
    16921 tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg ctgttgatgc
    16981 cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt acggcgccta
    17041 tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc caaaatgcgt
    17101 atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa tcggcataaa
    17161 gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc ggtttggatc
    17221 aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat tgccgatcag
    17281 ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa gcagcagatt
    17341 gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga aatcaagccg
    17401 ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa tggtaaagag
    17461 gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga tctgaaactt
    17521 atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg tctgcgccca
    17581 accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt gctgcgggaa
    17641 tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct ctacatcccg
    17701 gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat ttacggcatt
    17761 ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt gctggcggaa
    17821 cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca tgccgatatg
    17881 caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata tatcggcatt
    17941 gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc agaaaacttt
    18001 atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga ttctggctgg
    18061 gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt ctgtgaacct
    18121 atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa tctgtttaat
    18181 acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca gaaaaccctg
    18241 ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg gaaaacagcg
    18301 aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct ggtgagagca
    18361 tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga actggtttac
    18421 gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc ccgcgagctt
    18481 cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg cgctacgtta
    18541 gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat gcccggctgg
    18601 ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg ctgatttttt
    18661 catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat catctaccac
    18721 agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc cgtcatcgtt
    18781 gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg tgcgtcgatc
    18841 aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac cagtcaggat
    18901 gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca ggaacaggct
    18961 aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt tgatatcggt
    19021 tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc gcaacgactg
    19081 cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact ggcgacaacg
    19141 gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag tctgaaaaag
    19201 gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat ggatgaacta
    19261 cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga aaaggcgagc
    19321 gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc gcatgagggc
    19381 gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc agaaaccacg
    19441 ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc accttcccct
    19501 tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt atcacgcatc
    19561 tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg atattcctgt
    19621 gtctggtcta tttcgccaat gacatctatc acttggtatc cgcgccattg atcaagcagt
    19681 tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt acgccgatca
    19741 agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat caggtgtggg
    19801 catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg ctgctggttt
    19861 ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc tttccgctgg
    19921 catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc gacatcgcca
    19981 gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt gaagtgccgg
    20041 tggcaattgt gctgctgtgc tggatgggga ttacctcgtc agaagactta cgcaaaaaac
    20101 gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg ccggatgtct
    20161 tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt gtcttcttct
    20221 cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct gaagcagaaa
    20281 gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata tggagtacag
    20341 gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc gtgatgatgt
    20401 tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca ctaacctgcg
    20461 tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt caacggcggg
    20521 cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga ttattgagct
    20581 ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta accgcaactt
    20641 ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg ccgcagaatt
    20701 aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga cattgctgga
    20761 gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca cacgcgaaga
    20821 gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg tttgcgatga
    20881 acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga aattgctgat
    20941 cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat catcccggcg
    21001 caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc gtggagaaga
    21061 tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg ggattgcgtt
    21121 ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat taataagatt
    21181 aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct gaaagccttc
    21241 gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg gatcgacaat
    21301 gtctttcggt ttatataccg atagctgatg aattaccgcc gatgggacta tcgctggcga
    21361 cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg tggtatgaat
    21421 cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac tgactgcggt
    21481 acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat taaccgcctg
    21541 tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca gtaaatacca
    21601 ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa agctttacgc
    21661 taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta atttaacaaa
    21721 tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg ctacaatgga
    21781 cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc agggtgagct
    21841 aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg ctgaccgcac
    21901 tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact caatgccggt
    21961 gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc aggaagatgt
    22021 ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg agccgccaaa
    22081 aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa acatgccgac
    22141 aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg acgtcgatct
    22201 caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga ttacctgggg
    22261 gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt atcgccagca
    22321 gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg gcgcgctgga
    22381 ttatcaggag tggtgtgcgg cgcatccggg cgaacatttc ccggtttctg tggcgctggg
    22441 tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc tttcagagta
    22501 tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta tttccaatga
    22561 cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc ctggtgagat
    22621 ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg atagtttccc
    22681 ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt ccacctatac
    22741 cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag tgtttgtacc
    22801 gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg aaggctgttc
    22861 ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga agcgcgtcat
    22921 gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga tcgtttgcga
    22981 tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca cccgtatgga
    23041 cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg attttgcctc
    23101 gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat ggccggggga
    23161 aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg cgcatattga
    23221 cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat gcgcgtttgt
    23281 tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct gtaaagtgac
    23341 ctcggtagca gctatcacgg ataccgtata tcgtgtccgc atcgtgccag acgcggcctt
    23401 ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca aacgtccgtt
    23461 ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg gcgcttctga
    23521 aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc aaatcgtggt
    23581 cgaccttccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga tgattttgat
    23641 tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt tggcgcgtaa
    23701 cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc tgtatgatct
    23761 ctgcgagctt gaggcgcttt cgttaaagca ccttggtctg caagtggtgc cggtggttga
    23821 acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg tattgcagga
    23881 tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga tggcgaaaat
    23941 tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt ttggcgatgc
    24001 gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag aacggcaact
    24061 aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaattggtg cacgatgcct
    24121 gatgcgacgc tggcgcgtct tatcaggcct acattggtgc cggatcggta gaccggataa
    24181 ggcgttcacg ccgcatccgg caagtggtta aacccgctca aacaccgtcg caataccctg
    24241 acccagaccg atacacatcg tcgccagacc aaactgaacg tctttgcgtt ccatcagatt
    24301 cagcagcgtg gtgctgatac gcgcaccgga acaacccagc ggatgaccca gcgcgatcgc
    24361 accaccattg aggttgatct tctcgtcaat ctgctccatt agcccgagat ctttaatgca
    24421 cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac acgccgatat cgctggcaga
    24481 aagccccgct tttttcagcg ccagcttcga ggccggaacc gggccgtaac ccataatcga
    24541 tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg cgcggcttaa gacctaattc
    24601 atgggcgcgg ctttcactca tcaccagcat ggcagctgcg ccatcggaaa gtgcagaaga
    24661 tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc ggacgcagcg tggcgagggc
    24721 ttccacggtg gtttccgggc gaatcacttc gtcgtaatta aactgcttca ggacgccgtc
    24781 ggcatcgtga ccaccagttg ggatgatttc atttttaaat gcggccgact gcgtggcggc
    24841 ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc tgataccgtg
    24901 catacgcgcc agcatttctg ccgttaagcc catcatgccc gccgctttgg cgacattgcg
    24961 gctcaggccg gggtgaaaat cgacgccgtg actcatcggc acatggccta tatgctccac
    25021 gccgccaacc agacatgcct gcgcatcgcc agtcatgatc attcgtgctg cgtcatgcag
    25081 tgcctgcatg gatgaaccac acaagcgatt gacggtaacc gccgggacag agtgcggcac
    25141 ttctgccagc agcgccgcgt tacgggcgat gttaaaaccc tgctccagcg tctgttgcac
    25201 acaaccccag taaatatcgt cgagggccgc cgcttccagc gccgggttac gcgccagcag
    25261 gctacgcatt aaatgagcgg agagatcttc cgcacgcacg ttacgaaaag caccgccctt
    25321 cgaacggccc atcggggtgc gaattgcatc gacaatgaca acctgttcca ttgtgactcc
    25381 ttaagccgtt ttcaggtcgc caaccggacg ggctggctca accggaggat agtacggttc
    25441 gttatgacgc gctttattac gcagaccttc cggcacttca tacagcgggc cgaggtgctg
    25501 atattgctgt gccatatcga ggtattttgc gctaccgagg gtgtccagcc agcggaacgc
    25561 gccgccgtgg aacggaggga agcccaggcc gtagaccagc gccatatccg cttccgccgg
    25621 agtggcgata atgccctctt ccagacagcg caccacttcg ttgaccatcg ggatcatcat
    25681 gcgggcgata atctcttctt cgctgaaatc gcgcttcggt tggctcactt ctgccagcag
    25741 gtcttcaacg gcggcgtctt cttctttctt cggcttacct ttgctgtctt ctttataacg
    25801 ccagaaaccg aggccgttct tctgaccaaa gcggttggca tcaaacagcg cgtcgatggc
    25861 atcgcggtaa tctttctgca tccgctgcgg gaagcctgct gccatgacag cctgagcgtg
    25921 atgcgcggta tcaatgccca caacgtccag cagatatgcc gggcccatcg gccagccaaa
    25981 ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg ccgtcgcgca gcagctggct
    26041 gaaaccggcg aaatacggga acagcacgcg gttaacaaag aagccggggc agtcgttaac
    26101 cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca actttcgcga tggtttcgtc
    26161 ggagcttttc tcgccgcgaa taatttctac caacggcatt cggtggaccg ggttaaagaa
    26221 gtgcatcccg cagaagtttt ccgggcgttc cagcgcgttg gccagttcgc tgataggaat
    26281 ggttgaagtg ttagacgcca gcacggtatc ctggcgtact ttttgttcgg tttctgccag
    26341 tacggctttt ttcactttcg ggttttcaac aaccgcttct accacaatat ccacgcggtc
    26401 aaatccggcg tagtcgagcg ttgggtggat cgtggagatc acgccagcca gtttcagacc
    26461 atcgattttg ccgcgctcaa gctgcttgtt cagcagtttc gcggcttcgg tcatgccgag
    26521 ggttaacgac ttgtcgttga tatctttcat gacaaccggc acgcctttcc acgcggactg
    26581 gtaagcgata ccgccaccca tgatgcctgc gcccagcaca gcagcctgtt tcggggtttc
    26641 aacgtctttg gtgagtttct tcgctttgcc ttttacatat tgatcgttaa ggaaaatgcc
    26701 gaccagtgcg cgagcttcgt tggtatgcgc cagcgggaca aaacttttgt tttccagatt
    26761 taaggcttct tcacgaccaa aacgggccgc agcttcaatg gtttttactg cggtgatggg
    26821 ggccggataa tgtttccccg ccgtttgtgc gaccattcct ttagcgatgg tgaagctcat
    26881 ggtggcttca atcttgctca gttttagcgg ttcctgcttc ggctgacgtt tcgctttcca
    26941 gtcgaggtcg ccgttaatgg cctggcgtaa aatcgccatc gccccttcaa tcagtttttc
    27001 tgctttgact acgccatcca ccagaccgat tttcagcgcc tgatccgcgc cgacatcttt
    27061 accggcggca atgatttcca gtgcactgtc agcgcccagc atacgtggca tacgtacaga
    27121 accgccaaag cccggcatga tgcccagttt ggtttccggc agaccgatgc gcagatccgg
    27181 cgtcgccaga cgataatcgg tcgccagcac gcattcgcag ccaccaccca gcgcatagcc
    27241 gttgacggca gcaatggtcg gcaccggcag atcttccagg cgattaaaca cgctattggc
    27301 aaagtgcagc cactgactta actgttcttc aggaacgagg aacagggaca aaaattcggt
    27361 gatatcagca ccgacgataa aggctgcttt gttcgaacgc agcagcagcc cttttagatc
    27421 tgattgctgt tccagcacgc cgatggcctc gccgaggctg gcgacggtcg cagtgtcgag
    27481 tttattaact gaacctgggg catcaaatac cagttcggca atgccatctt ccagccagtc
    27541 aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca aggtgatctg
    27601 gtcgtaccag atgcatcgga gtgtgtgttt tgtgttaaaa atatgcaaat aaaagattaa
    27661 agaaatgccg ctctgatcac gctcggcaga aatcacgctc tggatgaacg atgtgctaag
    27721 atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact ggcctcgctc
    27781 tataaaaatc atatagctac cttacaagaa cggactcgcg atgcgctggc gcgcttcaag
    27841 ctggatgcat tacttattca ctccggcgag ctgttcaacg tttttctcga cgatcatccc
    27901 tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca ggtgccaaac
    27961 tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt ggttctatct gccggttgat
    28021 tactggcaca acgtcgaacc gctgccgacc tccttctgga ctgaagatgt ggaagtgatc
    28081 gcgctgccga aagccgatgg cattggtagc ctgctgcctg ctgcgcgcgg caatatcggt
    28141 tatatcggtc cggtgccgga acgtgcgctg caactgggta ttgaggccag caacatcaac
    28201 ccgaaagggg tgatcgacta cctgcattat taccgctcct tcaaaaccga gtacgaactg
    28261 gcctgtatgc gtgaagcgca gaaaatggcg gtcaatggtc atcgtgcggc agaagaagcg
    28321 ttccgttctg gcatgagcga gttcgatatc aatattgcct atctgaccgc gaccggtcat
    28381 cgtgataccg acgtacctta cagcaacatt gtggcgctta acgaacacgc tgcggtgttg
    28441 cattacacca aactggacca ccaggcaccg gaagagatgc gcagcttcct gctggatgcc
    28501 ggggccgaat ataacggcta tgcggctgac ctgacccgta cctggtcggc aaaaagcgac
    28561 aacgactacg cacagctggt gaaagacgta aatgatgaac aactggcgct gatcgccacc
    28621 atgaaagcag gcgtcagcta tgtggattac cacatccagt tccatcagcg catcgccaaa
    28681 ctgctgcgta aacatcaaat catcaccgat atgagtgaag aagcgatggt cgaaaacgat
    28741 ctcaccggac cgtttatgcc gcacggtatt ggccatccgc tgggcctgca ggtgcatgac
    28801 gtcgccggtt ttatgcagga tgatagcggt acacacctcg cggcaccggc aaaatatccg
    28861 tacctgcgct acacccgtat tctccagccg ggcatggtgt taaccatcga accgggtatc
    28921 tacttcattg aatcgcttct ggcaccgtgg cgtgaagggc agttcagcaa gcacttcaac
    28981 tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc gtatcgaaga caacgtggtg
    29041 atccacgaaa acaacgtgga aaacatgacc cgggatctga aactggcgtg atggaaagct
    29101 ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat caaaaagagc cgcttcataa
    29161 cgatgttggc gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa tcggtgcggg
    29221 cagaacaccc cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg ccggatgatt
    29281 ctcaacagct gggtttctct gacgacgggg agccggcggg aacggcaggt aaaccgatgc
    29341 tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc tgtggtagtg cgctactacg
    29401 gcggcatatt gcttggcacc ggtgggttag tgaaagcgta tggcggcggc gtgaatcagg
    29461 cgctgcgcca gctaacgacc caacgcaaga cgccattaac cgaatatact ttgcaatgtg
    29521 aatatagtca gttaaccggc attgaagcgt tgctggggca gtgtgacggc aaaattatca
    29581 acagtgatta tcaggcattc gttctgctgc gggtggcgct tccggcggcg aaagtggctg
    29641 aattttccgc aaagctggcg gattttagcc gtggttcatt gcaattgtta gcgattgaag
    29701 aataatcccc acttcgtttt gcagaactaa ggaagcggca gagatgcatt ttcgcgccat
    29761 tacccgaatc gttggactac tggtcatctt attttcaggg accatgatta tccctgggct
    29821 ggtagcactc atctaccggg atggagcggg ccgcgctttt acccagacct tttttgtcgc
    29881 cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa gagaagggcg aactgaaatc
    29941 ccgtgagggg tttctgatag tggtgctgtt ttggaccgtg ctgggtagtg tcggtgcgct
    30001 cccttttatc ttctcggaaa gcccgaacct cacgattacc gatgcgtttt ttgaatcttt
    30061 ctctggcctg accaccaccg gggccactac gctggtgggg ctggattcgc tccctcatgc
    30121 catcctcttt tatcgccaga tgctgcaatg gtttggcggg atggggatca tcgtgttagc
    30181 ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg cagaaatgcc
    30241 cggcccgctg aaagataaca aaatgcgccc gcgaattgcg gaaacggcga aaactctgtg
    30301 gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg ccggaatgga
    30361 tgcctttgat gccatcggcc atagctttgc gactatcgct attggcggct tctcgacaca
    30421 tgatgccagt atcggttatt tcgacagtcc gactattaac actatcattg ctatcttcct
    30481 gctgatctcc ggctgtaact acggtctgca cttttcactg ttaagtgggc gtagtctgaa
    30541 ggtttattgg cgcgatccgg aatttcgcat gtttatcggc gtacagttta cgctggtggt
    30601 tatttgtaca ctcgtactgt ggtttcataa tgtctacagt tcggcgctga tgacaattaa
    30661 ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc gggtttacga ctgacagcat
    30721 tgcccgctgg ccgctctttt tgccggtact gcttttatgt tcagctttta tcggcggttg
    30781 cgccgggtca acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt ttaagcaggg
    30841 gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta agctggggaa
    30901 tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga tttttctccg cctatgcatt
    30961 ggtgtttatt gtcagtatgc tggcgattat cgccacgggc gtggatgatt tttctgcctt
    31021 tgcctcagtt gttgcgacat tgaataacct ggggccaggg cttggcgtgg ttgctgataa
    31081 ctttaccagt atgaacccgg tggctaaatg gatcctgatt gccaacatgc tgtttggtcg
    31141 tctcgaggtc tttacattac tggtgctctt taccccgact ttctggcgtg aatgatggag
    31201 taatacgtga aaacattaat tcttttctca acaagggacg gacaaacgcg cgagattgcc
    31261 tcctacctgg cttcggaact gaaagaactg gggatccaga cggatgtcgc caatgtgcac
    31321 cgcattgaag aaccacaatg ggaaaactat gaccgtgtgg tcattggtgc ttctattcgc
    31381 tatggtcact accactctgc gttccaggaa tttgtcaaaa aacatgcgac gcgactgaat
    31441 tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga gaagcgtact
    31501 ccacagacca acagctacgc gcggaagttt ctgatgaact cgcaatggcg tcccgatcgc
    31561 tgcgcggtca ttgccggggc gctgcgttac ccacgttatc gctggtacga ccgttttatg
    31621 ctcaagctga ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga agttgtctat
    31681 accgattggg agcaggtggc gaatttcgcc cgagaaatcg cccatttaac cgacaaaccg
    31741 acgctgaaat aagcataaag aataaaaaat gcgcggtcag aaaattattt taaatttcct
    31801 cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac
    31861 acgccgccgg gtcagcgggg ttctcctgag aattccgaca gagaaagcaa aaataaatgc
    31921 ttgactctgt agcgggaaag cgtattatga acaccctgcg c
//
LOCUS       DAEBXD010000043        31766 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEBXD010000043 DAEBXD010000000
VERSION     DAEBXD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31766
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.043"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="KJL94_002045"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="KJL94_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBD4410398.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="KJL94_002046"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="KJL94_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBD4410399.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="KJL94_002047"
     CDS             2630..3391
                     /locus_tag="KJL94_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBD4410400.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="KJL94_002048"
     CDS             complement(3442..4326)
                     /locus_tag="KJL94_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBD4410401.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="KJL94_002049"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="KJL94_002049"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD4410402.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="KJL94_002050"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="KJL94_002050"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBD4410403.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="KJL94_002051"
     CDS             6202..6474
                     /locus_tag="KJL94_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBD4410404.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="KJL94_002052"
     CDS             6844..7920
                     /locus_tag="KJL94_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4410405.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="KJL94_002053"
     CDS             7947..9326
                     /locus_tag="KJL94_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410406.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="KJL94_002054"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="KJL94_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="KJL94_002055"
     CDS             10409..11245
                     /locus_tag="KJL94_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBD4410407.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="KJL94_002056"
     CDS             11250..12197
                     /locus_tag="KJL94_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBD4410408.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNEALDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="KJL94_002057"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="KJL94_002057"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBD4410409.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="KJL94_002058"
     CDS             13324..14082
                     /locus_tag="KJL94_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4410410.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="KJL94_002059"
     CDS             14200..15558
                     /locus_tag="KJL94_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410411.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="KJL94_002060"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="KJL94_002060"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBD4410412.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="KJL94_002061"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="KJL94_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBD4410413.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="KJL94_002062"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="KJL94_002062"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBD4410414.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="KJL94_002063"
     CDS             19503..20054
                     /locus_tag="KJL94_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBD4410415.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="KJL94_002064"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="KJL94_002064"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBD4410416.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVHARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="KJL94_002065"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="KJL94_002065"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBD4410417.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="KJL94_002066"
     CDS             23307..24347
                     /locus_tag="KJL94_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD4410418.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="KJL94_002067"
     CDS             complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="KJL94_002067"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBD4410419.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26043..26315
                     /locus_tag="KJL94_002068"
     CDS             26043..26315
                     /locus_tag="KJL94_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBD4410420.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="KJL94_002069"
     CDS             complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="KJL94_002069"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBD4410421.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26775..27395
                     /locus_tag="KJL94_002070"
     CDS             26775..27395
                     /locus_tag="KJL94_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD4410422.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="KJL94_002071"
                     /pseudo
     CDS             complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="KJL94_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28778..29431)
                     /locus_tag="KJL94_002072"
     CDS             complement(28778..29431)
                     /locus_tag="KJL94_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD4410423.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29431..30948)
                     /locus_tag="KJL94_002073"
                     /pseudo
     CDS             complement(29431..30948)
                     /locus_tag="KJL94_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30921..31766)
                     /locus_tag="KJL94_002074"
                     /pseudo
     CDS             complement(30921..31766)
                     /locus_tag="KJL94_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7957 a         7605 c         8240 g         7964 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcaaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgccgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc taggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatctg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atatggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gcttcgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatggggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaagcgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcacc atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcagca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatctg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca tgcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaaa ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
    25681 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
    25741 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct
    25801 gatccataga tataaaaacc ttatatatta atacgattgt gcttatgttg tttatgtcat
    25861 gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga
    25921 cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc
    25981 taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc
    26041 ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca ctggctaacc
    26101 cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc
    26161 agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat tctgaagggg
    26221 cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa acccttagca
    26281 ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc
    26341 aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc
    26401 atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg
    26461 gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt cgatgtccaa
    26521 ttcttcgttt aactcacgca ccagcacctg ctgctggctt tcatccagct cgactttacc
    26581 tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat
    26641 tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg
    26701 ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa aggctttata
    26761 tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg ttgttgcatc
    26821 agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca ttagtgggat
    26881 ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg gcggcgctag
    26941 tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag
    27001 ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt tacgcgctgg
    27061 tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt
    27121 tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg gcagcaaaac
    27181 atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg ggattaaccg
    27241 aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca tggtttgcgt
    27301 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
    27361 ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg ctactgcccg
    27421 ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg taccagccgc
    27481 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
    27541 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata ttccatgctt
    27601 tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta tagaaatctt
    27661 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
    27721 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
    27781 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
    27841 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
    27901 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
    27961 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
    28021 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
    28081 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
    28141 agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag gccgcttcaa
    28201 tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc aggtcatgca
    28261 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
    28321 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
    28381 cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
    28441 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag ctagcagaga
    28501 ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
    28561 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt tggtcaagtg
    28621 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
    28681 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
    28741 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
    28801 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
    28861 ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac tggcgaaaat
    28921 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
    28981 gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac aaagtggcag
    29041 gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac
    29101 gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga
    29161 actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt gcggtgggta
    29221 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccggcca
    29281 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
    29341 tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg ctttctggta
    29401 aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga cataacctac
    29461 ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca gttgccataa
    29521 cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg cctcagtggt
    29581 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
    29641 tgaaaatccc accgcgaagg cagtcagcac agggcgcaac atgagtgggc atttcacgta
    29701 aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc
    29761 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
    29821 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
    29881 gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca actggcgacg
    29941 ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg ttgctaccag
    30001 ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
    30061 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
    30121 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
    30181 gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt
    30241 tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg
    30301 gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa
    30361 tcgccagcat cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc
    30421 attgccagcg gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt
    30481 cgctcaatac tgccactaag atccacagca gaaatgcgct ttctttcact gcaagggtga
    30541 ggcccagacc gatccaaatc ggtccattgg cggagtgaaa tacgggaaat agtcatatag
    30601 cagccctccg tcagcaaaga gcagcagggc gctggtggca aaagccacat ggggaatggc
    30661 gagcagccac ggcagacggg cgcacatacg ctgccatttc ggcccaggcc atagcgccac
    30721 aatcaccagc agggcaatca acaatgcccc gaccgccgcg atggttgtcg acaccagcgt
    30781 tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagc gtgtcaacga
    30841 caacgcaggc gatatcagcg tgagagctgc cgggatcagc ggtgcataaa tcaccgccac
    30901 catcgcccac agcaaaaaga ttaatgcgta ccgtaacggc gtaaccattc ttgttccagt
    30961 gcattcaccc atcctgcgtg cggttcagcc agtaccggcg gcagatcctg cggcattctt
    31021 gattgcaata tttcgcgctg cccgtcaggc agtttttgcg gatcgagaac agaaggatcg
    31081 ccccagacaa cgggatctgc tttacgcagt tgcgcatcgg gtgagagcag gaaattggca
    31141 actaccttcg ccgcagcact ggcattcgcg ttggctggag tggtgacgaa atgcacgttg
    31201 ccaatcatcc cctcgcgaaa gccaaaactg taactgcttg ccggcaaatc gccgctggcg
    31261 attttttgct gcgcatgtgc ggggttaaag gtcagcgaca ggcgcaatgt gccggctttc
    31321 agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttccttcgcg ccacaaatac
    31381 ggatgcagtg catcaagata ttgctacaag ggcgcagtga cacgggcgaa agtcgcatcg
    31441 tccggcgctt cttttaatgc ggcgggatct ggcgtcagca taatcagcaa ctgttcaaga
    31501 aacgccgtgc cggtaaagtc cggtgggcgc ggataagtaa ccgtgccggg attggcttta
    31561 gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc gcggcgggcg
    31621 ataaacgtca gttgtgcgcc gccccagggc gattccgcac cttgtgtcgg cactgaaaaa
    31681 tctccccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt ctccgcccag
    31741 cccgtttgta ataaattggc ctcttt
//
LOCUS       DAEBXD010000044        30471 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEBXD010000044 DAEBXD010000000
VERSION     DAEBXD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30471
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.044"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..795
                     /locus_tag="KJL94_002075"
     CDS             <1..795
                     /locus_tag="KJL94_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4410424.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="KJL94_002076"
     CDS             806..1897
                     /locus_tag="KJL94_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4410425.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KJL94_002077"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KJL94_002077"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBD4410426.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="KJL94_002078"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="KJL94_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBD4410427.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="KJL94_002079"
     CDS             4859..5254
                     /locus_tag="KJL94_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBD4410428.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="KJL94_002080"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="KJL94_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBD4410429.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="KJL94_002081"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="KJL94_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBD4410430.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="KJL94_002082"
     CDS             complement(6563..7603)
                     /locus_tag="KJL94_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD4410431.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="KJL94_002083"
     CDS             complement(7676..8311)
                     /locus_tag="KJL94_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBD4410432.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="KJL94_002084"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="KJL94_002084"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBD4410433.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="KJL94_002085"
     CDS             complement(8937..9380)
                     /locus_tag="KJL94_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBD4410434.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="KJL94_002086"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="KJL94_002086"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBD4410435.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="KJL94_002087"
     CDS             complement(9720..10223)
                     /locus_tag="KJL94_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4410436.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="KJL94_002088"
     CDS             complement(10217..10741)
                     /locus_tag="KJL94_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBD4410437.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="KJL94_002089"
     CDS             10950..11945
                     /locus_tag="KJL94_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD4410438.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTSLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="KJL94_002090"
     CDS             11954..12832
                     /locus_tag="KJL94_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD4410439.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="KJL94_002091"
     CDS             12913..13920
                     /locus_tag="KJL94_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBD4410440.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KJL94_002092"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KJL94_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBD4410441.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KJL94_002093"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KJL94_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBD4410442.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KJL94_002094"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KJL94_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBD4410443.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KJL94_002095"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KJL94_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBD4410444.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KJL94_002096"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KJL94_002096"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBD4410445.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KJL94_002097"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KJL94_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBD4410446.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KJL94_002098"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KJL94_002098"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBD4410447.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KJL94_002099"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KJL94_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBD4410448.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KJL94_002100"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KJL94_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBD4410449.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KJL94_002101"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KJL94_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBD4410450.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KJL94_002102"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KJL94_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBD4410451.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="KJL94_002103"
     tRNA            complement(27654..27730)
                     /locus_tag="KJL94_002103"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="KJL94_002104"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="KJL94_002104"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBD4410452.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..>30471)
                     /locus_tag="KJL94_002105"
     CDS             complement(29429..>30471)
                     /locus_tag="KJL94_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD4410453.1"
                     /translation="AAKEHQRLLSIANTVPYFQLSVRDTSIDTYVLIVGESVRVDNMS
                     LYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTADSVLSHDIHNYPDNII
                     NMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFDELLLPHLSQALQQNT
                     QQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIHYTDSLLGQVFELLKD
                     RRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWYSPVLGDGVDRTTENN
                     IFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDANHDVFDYVMLRKEFTE
                     DKQGNPTPEGQG"
BASE COUNT         7327 a         8314 c         7629 g         7201 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgc
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcagt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa cctcccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tcgtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaagctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagttaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct a
//
LOCUS       DAEBXD010000045        29236 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEBXD010000045 DAEBXD010000000
VERSION     DAEBXD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.045"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..218
                     /locus_tag="KJL94_002106"
     CDS             <1..218
                     /locus_tag="KJL94_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4410454.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KJL94_002107"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KJL94_002107"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD4410455.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRSASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KJL94_002108"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KJL94_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBD4410456.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="KJL94_002109"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="KJL94_002109"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBD4410457.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="KJL94_002110"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="KJL94_002110"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBD4410458.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="KJL94_002111"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="KJL94_002111"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBD4410459.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="KJL94_002112"
     CDS             7229..8179
                     /locus_tag="KJL94_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBD4410460.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="KJL94_002113"
     CDS             complement(8220..8690)
                     /locus_tag="KJL94_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBD4410461.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="KJL94_002114"
     CDS             complement(8745..9947)
                     /locus_tag="KJL94_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBD4410462.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="KJL94_002115"
     CDS             complement(10034..10576)
                     /locus_tag="KJL94_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410463.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="KJL94_002116"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="KJL94_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBD4410464.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="KJL94_002117"
     CDS             complement(11319..11921)
                     /locus_tag="KJL94_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD4410465.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="KJL94_002118"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="KJL94_002118"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBD4410466.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="KJL94_002119"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="KJL94_002119"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBD4410467.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="KJL94_002120"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="KJL94_002120"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBD4410468.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="KJL94_002121"
     CDS             16319..17209
                     /locus_tag="KJL94_002121"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBD4410469.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="KJL94_002122"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="KJL94_002122"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBD4410470.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLVLAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="KJL94_002123"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="KJL94_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBD4410471.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="KJL94_002124"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="KJL94_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBD4410472.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KJL94_002125"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KJL94_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBD4410473.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KJL94_002126"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KJL94_002126"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBD4410474.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KJL94_002127"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KJL94_002127"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBD4410475.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KJL94_002128"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KJL94_002128"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBD4410476.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KJL94_002129"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KJL94_002129"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBD4410477.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KJL94_002130"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KJL94_002130"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBD4410478.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KJL94_002131"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KJL94_002131"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBD4410479.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KJL94_002132"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KJL94_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBD4410480.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="KJL94_002133"
     CDS             complement(27384..27845)
                     /locus_tag="KJL94_002133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4410481.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="KJL94_002134"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="KJL94_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBD4410482.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="KJL94_002135"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="KJL94_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7086 a         7855 c         7300 g         6995 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcactgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgtcctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAEBXD010000046        28426 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEBXD010000046 DAEBXD010000000
VERSION     DAEBXD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28426
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.046"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            564..1334
                     /locus_tag="KJL94_002136"
     CDS             564..1334
                     /locus_tag="KJL94_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBD4410483.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="KJL94_002137"
     CDS             1325..1798
                     /locus_tag="KJL94_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBD4410484.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="KJL94_002138"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="KJL94_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBD4410485.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="KJL94_002139"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="KJL94_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBD4410486.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="KJL94_002140"
     CDS             3233..3727
                     /locus_tag="KJL94_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBD4410487.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="KJL94_002141"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="KJL94_002141"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBD4410488.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KJL94_002142"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KJL94_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="KJL94_002143"
     CDS             7571..8485
                     /locus_tag="KJL94_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD4410489.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="KJL94_002144"
     CDS             8700..10061
                     /locus_tag="KJL94_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410490.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KJL94_002145"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KJL94_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBD4410491.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KJL94_002146"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KJL94_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBD4410492.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="KJL94_002147"
     CDS             12611..13900
                     /locus_tag="KJL94_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBD4410493.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="KJL94_002148"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="KJL94_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBD4410494.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16204
                     /gene="hpaD"
                     /locus_tag="KJL94_002149"
     CDS             15365..16204
                     /gene="hpaD"
                     /locus_tag="KJL94_002149"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBD4410495.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRTI
                     LKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWREGQFK
                     EFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVNAVFP
                     LPA"
     gene            16214..16594
                     /locus_tag="KJL94_002150"
     CDS             16214..16594
                     /locus_tag="KJL94_002150"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBD4410496.1"
                     /translation="MPHFIVECTENIREEARLPGLFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16662..17465
                     /gene="hpaH"
                     /locus_tag="KJL94_002151"
     CDS             16662..17465
                     /gene="hpaH"
                     /locus_tag="KJL94_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBD4410497.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17476..18279
                     /gene="hpaI"
                     /locus_tag="KJL94_002152"
     CDS             17476..18279
                     /gene="hpaI"
                     /locus_tag="KJL94_002152"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBD4410498.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18301..19656
                     /gene="hpaX"
                     /locus_tag="KJL94_002153"
     CDS             18301..19656
                     /gene="hpaX"
                     /locus_tag="KJL94_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBD4410499.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19666..20556
                     /gene="hpaA"
                     /locus_tag="KJL94_002154"
     CDS             19666..20556
                     /gene="hpaA"
                     /locus_tag="KJL94_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBD4410500.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20583..21520
                     /locus_tag="KJL94_002155"
                     /pseudo
     CDS             20583..21520
                     /locus_tag="KJL94_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21747..23305
                     /gene="hpaB"
                     /locus_tag="KJL94_002156"
                     /pseudo
     CDS             21747..23305
                     /gene="hpaB"
                     /locus_tag="KJL94_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23323..23835
                     /locus_tag="KJL94_002157"
     CDS             23323..23835
                     /locus_tag="KJL94_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBD4410501.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24228..26378
                     /gene="btsT"
                     /locus_tag="KJL94_002158"
     CDS             24228..26378
                     /gene="btsT"
                     /locus_tag="KJL94_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBD4410502.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26428..26631
                     /locus_tag="KJL94_002159"
     CDS             26428..26631
                     /locus_tag="KJL94_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD4410503.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26642..27598
                     /gene="yjiA"
                     /locus_tag="KJL94_002160"
     CDS             26642..27598
                     /gene="yjiA"
                     /locus_tag="KJL94_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBD4410504.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27667..>28426)
                     /locus_tag="KJL94_002161"
     CDS             complement(27667..>28426)
                     /locus_tag="KJL94_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBD4410505.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6958 a         7430 c         7122 g         6916 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcac
    15841 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    15901 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    15961 gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga
    16021 gttttgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16081 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16141 taccgagctg ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc
    16201 gtaaggacgt tttatgccgc actttatcgt ggaatgtact gaaaatattc gtgaagaagc
    16261 ccgcttacct gggctgtttg ccagtgttaa cactgcactg gcggcaacgg ggatttttcc
    16321 gcttggggga atacgtagcc gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa
    16381 gcacgattac gcctttgtgc atatgacgct aaaaatcggc agcggacgca gtctggaaag
    16441 ccgtcaggaa gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg cctcactgat
    16501 ggagagccgt tacctagcgc tttcatttga gattgcagag ctgcacccga cgttgaattt
    16561 caaacagaac aacgtacacg cgttgtttaa ataaatttgc accagcttcc aggccggatg
    16621 ataaggcgct gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc
    16681 acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga
    16741 tctcgctgga ttacccggag atcaccatcg aagacgccta cgccgtgcag cgcgaatggg
    16801 ttcgcctgaa aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga
    16861 aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggtgcactg atggacgaca
    16921 tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag
    16981 tggagctggc ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg
    17041 tttacaacgc cacggactat gtgatcccgg cgctggagct gatcgacgct cgctgccaca
    17101 acatcgatcc ggaaacccag cgcccgcgca aagtattcga caccatttcc gataacgccg
    17161 ccaacgccgg agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt
    17221 ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg
    17281 tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg
    17341 tacaactgga agccgggcaa atcattctcg gtggttcgtt cacccgcccg gttccggcgc
    17401 gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg
    17461 tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag
    17521 attggattat ggctggggct gagcagcagc tacagcgcgg agttactggc cggaccagga
    17581 ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc
    17641 caactacagg cgattgcgcc ctaccccagc cagccagtgg tacgcccatc gtggaacgat
    17701 ccggtgcaaa tcaaacaact gctggacgta ggcacacaaa ccttactggt gccgatggta
    17761 caaaacgccg acgaagcccg taaagcggta cgcgccaccc gttatccccc cgccagtatt
    17821 cgcggtgtgg gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg
    17881 caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag
    17941 aacttaccgc agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat
    18001 ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt
    18061 gagcaggcga tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat
    18121 gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac
    18181 accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa tagttcttct
    18241 atttcgatgg cgaagcaaaa caacaactct gtctactaaa cgtacgatgt ggagcgcatc
    18301 atgacgacct ctaccctgca atataaagat aataaaactg ttgaaattga acatcgtgtc
    18361 attaataaat tatttcgtcg cctaatcatt tttcttttta ttctgtttgt tttttcgttt
    18421 cttgatcgca tcaatatagg atttgccgga ctgacaatgg gcaaggatct tggcctgacg
    18481 tccacaatgt ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc
    18541 cccagtaata tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg
    18601 gtctggggaa ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac actctatcta
    18661 ctgcgtatgc tggttggtat tgctgaggcg ggttttttac ctggtatttt ggtttatctg
    18721 acctggtggt tcccggcata tcatcgggcc agagccaatg ccttatttat gatagcgatg
    18781 ccagtcacta tgatgctggg attgatcctt tcaggctaca ttctggcctt ggatggactg
    18841 tggaatctaa aaggctggca atggctcttt ttgctggaag gtctgccatc ggtcattctt
    18901 ggtgtcgtca cctggtttta tctcaacgac acaccagacc aggcgacctg gcttgatgaa
    18961 aacgaaaaac aggctttaaa aacgatgatt ctccgtgaac aagagagtgc atttgcccat
    19021 gcatctgttc cacgctcaac cttacgtgaa gtcctgaccc cagccatcat gctatataca
    19081 cttgcttatt tttgcctgac taatactctg agtgcgatta atatctggac gccgcaaatc
    19141 ctacagagtt tcaatactgg cagtagcaac atcatgattg gtttgctggc ggccatcccc
    19201 caattctgca caatttttgg catgatctgg tggagtcgtc gttccgaccg tcttaaagaa
    19261 cgcaaaaagc atacgattct tccctacctg tttgccgctg ccggatggtt actcgcttct
    19321 gcgacagatc acagcctcat tcaactgagc agcatcatta tggcctcaat gggatcattt
    19381 accgcgatga ctatcttttg gacaacaccg gatcaagtta taagcttgca atcgcgtgcc
    19441 gttgcgatgg ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag tccattactt
    19501 atcggtattt tgaaagatgc aaccggcagt tttagctcag gtttgtggtt tgtagcagga
    19561 ttgctggtac tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg ggaacactct
    19621 ctttcatctg aacttatcgc accaaaaccg caataaggag tcatgatgtc cagtgaagct
    19681 atctttgaac atggttatga aaatattgat ttggtccggg aatatgatgc ccgctacgct
    19741 ggtgaagatg tgcattatga aacatttgcc cggttagcga atttctttgg acgcgatatg
    19801 cgccctcact ggcacgatag atattttcaa ctccattatc tggtcactgg acgcattact
    19861 ttacaactgg atgagcactt ctattctgtg cgcgcaccgc tttttgtact aacaccacca
    19921 tcggtgccac atactttctt tactgatcct gatagcgatg ggcatgttct gaccgtacgt
    19981 caggagttaa tttggcccct ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg
    20041 ctacaaggta tctgtctgtc actggaggga atggaacata cacaggcggc attaaatcat
    20101 tattggccga taatatccct ggagtttcaa caaaataagc aaggacggga aattctattg
    20161 caatcactgg cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc aaatgatatt
    20221 tcaacatgca gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg gctgattgac
    20281 gaaaaatatc gccaacattt aatggtccct gaatatgcaa caatgctcgg tatgagtgaa
    20341 tcacggctta ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag attgattttc
    20401 gaacgagtga gccgggaagc cagacggctt ctgctctata gcgagcaaag catcaaccag
    20461 atagcccttg atcttggcta caaagatccc gcctatttcg cccgtttttt caaccgaatg
    20521 atgggttgcc caccaacaca attccgtggt cgctgaccat ttatcaacct gagagactcg
    20581 acatgaaaat atctttacgc tcactatttg tggcgtgcct gattgcgcca gtggcattca
    20641 cctacgcctc cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa ggtggccctt
    20701 ctttattaaa agctgaccgt ttaccgatgg caacggcact tattattggc aataaaacct
    20761 agttaattga tgctggttac ggggccagct tacaactggt aaaaaataat attccactgc
    20821 gccagattga taatatttta ctcacccatt tacacagtga tcatattctg gactatccat
    20881 cagtcttaat gaatgcatgg gctagcggtc tgcaggacaa aactattcat gtattcggtc
    20941 cgccaggcac ccgagccatg accgctgcca gttggcaggt cttcgatcgg gacatcacac
    21001 tgcgtatgga agaagaagga aaacccgatc ctcgcaaact cgttacagct caagatgttg
    21061 gtgaaggcgt agtttataaa gatgataccg tgaccgtgac tgcattaaag gtccctcatt
    21121 ctcctttccc tgacggtgag gcttttgctt atcgcttcga cacacaaggc aagcgaattg
    21181 tcttctcagg agatacctcg tactttcccc cactggcaac tttctcagaa ggcgctgata
    21241 tacttgtgca tgaggccgta catgagccct ccgtcgctaa gctggcagac agtattggca
    21301 atggtaaaac actggcaaaa gccattgctt ctcaccatac gactattgag gatgtaggca
    21361 aaattgccaa tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta ccaatcaccg
    21421 ttccggatga aacatggaag caagaagcac agaaaactat tcaggcccta tcgtcgttgg
    21481 tcatgaaaat atgacgatta ccattccatt cgccccataa aaaattccgt ccccgttagc
    21541 ccttgttagc ggggaatatt atgatttgcc ttactgtaag ttgatcacga tcgcagttta
    21601 ctatttaact ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg
    21661 acaaccgcgt tcaacgcttc aattaaataa caaaaacaac acacaaaatt aacaatcgta
    21721 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
    21781 gtcctttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
    21841 atggcgagcg agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg
    21901 ttgcccaact gtacgacgcg ctgcacaaac cggagatgca ggactctctg tgttggaaca
    21961 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg
    22021 acctgcgcca gcaacgcgac gccatcgctg aatggtcacg cctgagctat ggctggatgg
    22081 gccgtacccc agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt
    22141 acggtcagtt cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct
    22201 actttaacca cgcgattgtt aacccaccga tcgtcatttg ccgaccgata aagtgaaaga
    22261 cgtttacatc aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt
    22321 ggttgccacc aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt
    22381 gatgggcgaa aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt
    22441 gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga
    22501 ctacccgctc tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact
    22561 gatcccatgg gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat
    22621 ggaaggcggt ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact
    22681 cgacttcatt acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg
    22741 tggtgtgcag gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag
    22801 tgactcgatg tgttcagaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc
    22861 cgcactgcaa acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat
    22921 agaacgcaac gttaccagtg gcctgatcta cctcccttcc agtgcccgtg acctgaacaa
    22981 tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt
    23041 ccagcgcatc aagatcctca aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg
    23101 tcacgagctg tatgaaatca actactccgg tagccaggat gagattcgcc tgcaatgtct
    23161 gcgccaggca caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct
    23221 gtcggaatac gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa
    23281 catgctggat aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa
    23341 cgcctgcgct ttcatgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc
    23401 gagggcgacg ccggacaatg cgggattacg gcaacggctg tctgctcggt cacggataca
    23461 ccaccgtcgc tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc
    23521 aacggcaagt tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc gcgccacttc
    23581 gcgggcatga caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt
    23641 ccgctggcgc agccggtgct aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat
    23701 gtgcaggcga ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt
    23761 gcagaaggtc acggacttat ttactttaaa cgccgtttcc atccggtgat gctggaaatg
    23821 gaagctgcga tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg
    23881 atctcaatca catatctacc cgatgaataa ttcttgccca accgctcacc gccattcctg
    23941 accgcttaca tccctaaaat aaccactcag ttatttacct tactttacgc gcacgtatct
    24001 ctggcaacat cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag
    24061 aacggcttcg gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc
    24121 aagtgagtga atccccggga gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg
    24181 ccgcacctgt aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa
    24241 aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
    24301 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
    24361 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
    24421 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
    24481 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
    24541 tgggaccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
    24601 gggtagtgct ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta
    24661 acggcgcatc tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg
    24721 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg
    24781 ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg
    24841 cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg
    24901 ttattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    24961 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    25021 atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg
    25081 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
    25141 cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag
    25201 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    25261 cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca
    25321 ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt
    25381 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca
    25441 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga
    25501 aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag
    25561 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    25621 gtgcgccgac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    25681 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
    25741 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    25801 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    25861 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    25921 tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca
    25981 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
    26041 tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc
    26101 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    26161 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    26221 caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    26281 cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
    26341 tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc
    26401 ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat
    26461 ctcggccagg cggcgaagat gttgattggt attccagact acgacaacta cgtcgagcat
    26521 atgaagacca accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc
    26581 cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag
    26641 catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca aaactaccct
    26701 gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt
    26761 cggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga tcaaaacgct
    26821 gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaggacgcac tactcgacct
    26881 gctggataat ctcgacaagg gcaatattca gttcgatcgt ctggtgattg aatgcaccgg
    26941 catggccgat cccggcccga ttattcagac ctttttctcc catgagattt tatgccagct
    27001 ctacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa
    27061 ccagttcacc atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac
    27121 cgacgtcgca ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc
    27181 accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg
    27241 ttttatgctg gaagagaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa
    27301 acaaaacgat atttcgtcaa ttgtggtgga actggattac ccggtagata tcagcgaagt
    27361 ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg
    27421 gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta
    27481 cagcgccgac tgggacaggc cgtggggcga tgaaaaaccg catagcacaa tggtgtttat
    27541 cgggattcag ttgccggagg aagagattcg ggcggcgttt gcgggattga ggaagtaagt
    27601 tcgggaaaga tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta
    27661 gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt
    27721 cagttcagcc aatccccatt ctgaagaatt acaaacatct tttgttaaat aaaaagagct
    27781 tttactatta agtttctcat aaatagattt ctttttgata aaatcatagt gttgtgtttc
    27841 agaatttttg gaaccactta tgagtgttaa attacctatt ttatgcagcc agtagacatg
    27901 ctcttgaggt tggaatctat taatccagta ttcattaact aatgcttgag gaagaatatg
    27961 ctcaattgtg attctccccg tataggtttt aatcacgctc tcatcttgtt gctccatatc
    28021 taagcgaagc aaaattgctt ttattaagtt cacttgattc ggacgaggct catataaatc
    28081 ttcatccaga gcagctataa aacctgaatt atctgcatgt tggtttatct gatttatcac
    28141 agaatcaaaa ggcatatcat tatttattgc taccagagca gaataacaaa ccatttctct
    28201 ttggctttta atttgttttt ttaaccaccc atgcatatat accttctcaa aggcggtaat
    28261 aaattgagaa aaatcatcta aattaaaatc ttctgtacga gccattcgat tcatgaatgc
    28321 cataaccgga ggaatccact catctacccc taaattactt agcgatgcta tttttcgtcg
    28381 tattgaaggg tgttcaaaat cattctctaa aatcttagta taattt
//
LOCUS       DAEBXD010000047        28338 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEBXD010000047 DAEBXD010000000
VERSION     DAEBXD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.047"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KJL94_002162"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KJL94_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="KJL94_002163"
     CDS             complement(998..2020)
                     /locus_tag="KJL94_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4410506.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="KJL94_002164"
     CDS             complement(2034..3536)
                     /locus_tag="KJL94_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD4410507.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KJL94_002165"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KJL94_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="KJL94_002166"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="KJL94_002166"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBD4410508.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="KJL94_002167"
     CDS             complement(5851..6192)
                     /locus_tag="KJL94_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBD4410509.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KJL94_002168"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KJL94_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBD4410510.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KJL94_002169"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KJL94_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBD4410511.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="KJL94_002170"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="KJL94_002170"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBD4410512.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="KJL94_002171"
     CDS             12871..14214
                     /locus_tag="KJL94_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD4410513.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="KJL94_002172"
     CDS             complement(14276..14482)
                     /locus_tag="KJL94_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBD4410514.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="KJL94_002173"
     CDS             14807..15361
                     /locus_tag="KJL94_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD4410515.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="KJL94_002174"
     CDS             complement(15424..16362)
                     /locus_tag="KJL94_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410516.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KJL94_002175"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KJL94_002175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBD4410517.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="KJL94_002176"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="KJL94_002176"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBD4410518.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="KJL94_002177"
     CDS             complement(19573..19953)
                     /locus_tag="KJL94_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD4410519.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="KJL94_002178"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="KJL94_002178"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD4410520.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="KJL94_002179"
     CDS             21011..21976
                     /locus_tag="KJL94_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4410521.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="KJL94_002180"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="KJL94_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBD4410522.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KJL94_002181"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KJL94_002181"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBD4410523.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KJL94_002182"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KJL94_002182"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD4410524.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="KJL94_002183"
     CDS             25350..25895
                     /locus_tag="KJL94_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBD4410525.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="KJL94_002184"
     CDS             complement(25879..26526)
                     /locus_tag="KJL94_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4410526.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="KJL94_002185"
     CDS             26738..27529
                     /locus_tag="KJL94_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBD4410527.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="KJL94_002186"
     CDS             27539..>28338
                     /locus_tag="KJL94_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBD4410528.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7542 c         6989 g         6854 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAEBXD010000048        27760 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEBXD010000048 DAEBXD010000000
VERSION     DAEBXD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27760
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.048"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            252..2273
                     /gene="uvrB"
                     /locus_tag="KJL94_002187"
     CDS             252..2273
                     /gene="uvrB"
                     /locus_tag="KJL94_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBD4410529.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2465..3373)
                     /gene="yvcK"
                     /locus_tag="KJL94_002188"
     CDS             complement(2465..3373)
                     /gene="yvcK"
                     /locus_tag="KJL94_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBD4410530.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3638..3782
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3770..4759
                     /gene="moaA"
                     /locus_tag="KJL94_002189"
     CDS             3770..4759
                     /gene="moaA"
                     /locus_tag="KJL94_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBD4410531.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4781..5293
                     /gene="moaB"
                     /locus_tag="KJL94_002190"
     CDS             4781..5293
                     /gene="moaB"
                     /locus_tag="KJL94_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBD4410532.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5296..5781
                     /gene="moaC"
                     /locus_tag="KJL94_002191"
     CDS             5296..5781
                     /gene="moaC"
                     /locus_tag="KJL94_002191"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBD4410533.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5774..6019
                     /gene="moaD"
                     /locus_tag="KJL94_002192"
     CDS             5774..6019
                     /gene="moaD"
                     /locus_tag="KJL94_002192"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBD4410534.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6021..6473
                     /gene="moaE"
                     /locus_tag="KJL94_002193"
     CDS             6021..6473
                     /gene="moaE"
                     /locus_tag="KJL94_002193"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBD4410535.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            6610..7314
                     /locus_tag="KJL94_002194"
     CDS             6610..7314
                     /locus_tag="KJL94_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD4410536.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7519..8232
                     /locus_tag="KJL94_002195"
     CDS             7519..8232
                     /locus_tag="KJL94_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD4410537.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(8268..9224)
                     /locus_tag="KJL94_002196"
     CDS             complement(8268..9224)
                     /locus_tag="KJL94_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBD4410538.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(9224..10465)
                     /gene="clsB"
                     /locus_tag="KJL94_002197"
     CDS             complement(9224..10465)
                     /gene="clsB"
                     /locus_tag="KJL94_002197"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBD4410539.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(10462..11223)
                     /locus_tag="KJL94_002198"
     CDS             complement(10462..11223)
                     /locus_tag="KJL94_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD4410540.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADIMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11356..11766
                     /locus_tag="KJL94_002199"
     CDS             11356..11766
                     /locus_tag="KJL94_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBD4410541.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11728..12834)
                     /locus_tag="KJL94_002200"
     CDS             complement(11728..12834)
                     /locus_tag="KJL94_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4410542.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12845..13978)
                     /locus_tag="KJL94_002201"
     CDS             complement(12845..13978)
                     /locus_tag="KJL94_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4410543.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(13971..15707)
                     /locus_tag="KJL94_002202"
     CDS             complement(13971..15707)
                     /locus_tag="KJL94_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4410544.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15700..16695)
                     /gene="hlyD"
                     /locus_tag="KJL94_002203"
     CDS             complement(15700..16695)
                     /gene="hlyD"
                     /locus_tag="KJL94_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBD4410545.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(16698..17369)
                     /gene="cecR"
                     /locus_tag="KJL94_002204"
     CDS             complement(16698..17369)
                     /gene="cecR"
                     /locus_tag="KJL94_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBD4410546.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17598..18785
                     /gene="rhlE"
                     /locus_tag="KJL94_002205"
                     /pseudo
     CDS             17598..18785
                     /gene="rhlE"
                     /locus_tag="KJL94_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(18992..19474)
                     /gene="ybiA"
                     /locus_tag="KJL94_002206"
     CDS             complement(18992..19474)
                     /gene="ybiA"
                     /locus_tag="KJL94_002206"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBD4410547.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            19594..21744
                     /gene="dinG"
                     /locus_tag="KJL94_002207"
     CDS             19594..21744
                     /gene="dinG"
                     /locus_tag="KJL94_002207"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBD4410548.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            21772..22734
                     /gene="ybiB"
                     /locus_tag="KJL94_002208"
     CDS             21772..22734
                     /gene="ybiB"
                     /locus_tag="KJL94_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBD4410549.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            22875..23960
                     /gene="hcxB"
                     /locus_tag="KJL94_002209"
     CDS             22875..23960
                     /gene="hcxB"
                     /locus_tag="KJL94_002209"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBD4410550.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(24189..24449)
                     /locus_tag="KJL94_002210"
     CDS             complement(24189..24449)
                     /locus_tag="KJL94_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4410551.1"
                     /translation="MKTINTVVAAMALSTLSFGVFATEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(24714..24980)
                     /gene="ybiI"
                     /locus_tag="KJL94_002211"
     CDS             complement(24714..24980)
                     /gene="ybiI"
                     /locus_tag="KJL94_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBD4410552.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(25054..25731)
                     /gene="ybiX"
                     /locus_tag="KJL94_002212"
     CDS             complement(25054..25731)
                     /gene="ybiX"
                     /locus_tag="KJL94_002212"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBD4410553.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(25773..27760)
                     /locus_tag="KJL94_002213"
                     /pseudo
     CDS             complement(25773..27760)
                     /locus_tag="KJL94_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         6530 a         7175 c         7450 g         6605 t
ORIGIN      
        1 cttgccataa aactgtcatc actcatcttg acaaatgtta aaaaagccgt tgctttgggg
       61 ataacccggt aaggctggag ttttatctcg ccacagagta aattttgctc atgattgaca
      121 gcggagttta cgctgtatca gaaatattat ggtgatgaac tgttttttta tccagtataa
      181 tttgttggca taattaagta cgacgagtaa aattacatac ctgcccgccc aactccttca
      241 ggtagcgact catgagtaaa ccgttcaaac tgaattccgc ttttaaacct tctggcgatc
      301 agccagaggc gattcgacgt ctcgaagagg ggctggaaga tggcctagcg caccagacgt
      361 tacttggcgt gactggctcc gggaaaacct tcaccattgc caatgtcatt gctgaccttc
      421 agcgcccaac catggtactt gcgcccaaca aaacgctggc ggcccagctg tatggcgaaa
      481 tgaaagagtt cttcccggaa aacgcggtgg agtatttcgt ctcctactac gactactatc
      541 agccggaagc ctatgtaccg agttccgaca ccttcattga gaaagatgcc tcggttaacg
      601 aacatattga gcagatgcgt ttgtccgcca ccaaagcgat gctggagcgg cgtgatgtga
      661 ttgtagtggc gtctgtttcc gcgatttatg gtctgggcga tcctgattta tatctcaaga
      721 tgatgctcca tctcacggtc ggtatgatta tcgatcagcg cgcgattctg cgccgactgg
      781 cggagctgca atacgctcgt aatgatcaag cattccagcg tggtactttc cgcgttcgtg
      841 gcgaggtgat agatatcttc ccggcagaat cggatgacat tgcacttcgc gtggaactgt
      901 ttgacgagga agtggaacga ttgtcgttat ttgacccgct gaccgggcag attgtttcca
      961 ctattccacg ttttaccatc tacccgaaaa cgcactacgt cacaccgcgc gagcgcatcg
     1021 tacaggcgat ggaggagatc aaagaagaac tggccgccag acgcaaagtg ctgttggaaa
     1081 acaacaaact gctggaagag cagcggctga cccagcgtac ccagtttgat ctggagatga
     1141 tggacgagct gggctactgt tcggggattg aaaactactc gcgcttcctc tccggtcgtg
     1201 gaccgggtga gccaccgccg acgctgtttg attacctgcc tgccgatggg ctgctggtcg
     1261 tcgatgaatc tcacgtcacc attccacaaa ttggcggcat gtatcgcggt gaccgggcgc
     1321 gtaaagagac actggtggag tacggcttcc gcctgccatc agcgctggat aaccgtccgc
     1381 ttaagtttga agagttcgaa gcattagcgc cgcaaaccat ctatgtttcg gcgacgccgg
     1441 gtaattacga gctggaaaaa tccggcggcg atgtggtgga tcaggtggtg cgtccaaccg
     1501 gattgcttga cccgattatc gaagtgcggc cggtggcgac acaggttgat gatcttcttt
     1561 cggagattcg tcagcgagcg gcaattaacg aacgcgtgct ggtcaccaca ctgaccaagc
     1621 gaatggcgga agatcttgcc gaatatctcg aagaacacgg tgagcgcgtg cgttatcttc
     1681 actcagatat cgacaccgtc gagcgtatgg agattatccg cgacttgcgt ctgggtgagt
     1741 tcgacgtatt ggtagggatc aacttactgc gtgaaggtct ggatatgccg gaagtgtcgc
     1801 tggtggcgat cctcgacgct gacaaagaag gcttcctgcg ttccgaacgt tcgttgatcc
     1861 agaccattgg tcgtgcggca cgtaacgtta acggtaaagc gattctctac ggcgatagga
     1921 gcactccatc aatggcgaaa gcgattggcg aaaccgaacg tcgccgtgag aaacagcaga
     1981 agtacaacga ggaacacggc attacgccgc aaggcttgaa caagaaagtg gtcgatatcc
     2041 tggcgctggg gcagaacatt gccaaaacca aagcgaaggg cagaggaaaa tcgcgcccga
     2101 ttgttgagcc ggataatgtg ccgatggata tgttgccgaa agcgttgcag cagaaaatcc
     2161 atgaactgga agggctgatg atgcaacacg cgcagaatct ggagttcgaa gaagcggcac
     2221 aaattcgtga ccagttgcat cagttgcgtg agttgtttat tgccgcatcg taacaggata
     2281 gcgaagaaga ctgatgacaa acggaaaaca gtctgatgcg ctacgcttat caggcctaca
     2341 ttttctccgc aatatattga atttgcgcgg tttgtaggcc ggataaggcg ttcacgccgc
     2401 atccggcata aacaacgcgc actttgtctt caataggaag ccggaatttt ccttcggatt
     2461 tccgttaacc taaagcctgt aacgcctttt ccagcgcgtt atgtaacaac tggcggtcat
     2521 gacgatacgg aatatcgctg gcctccagta cctcctggat cacaatccgc tctttcaccg
     2581 ccgagacatc cacttttggc ccgacgatga ccgcatcaat gaccttttta ccaacatact
     2641 gctccataat tgccagcttg ctttccagct tcaaattagc cgcaggtaaa ctcaactcac
     2701 gccccagatt gccgatataa accatcggcg ctggcgtgcg gcgtaatgcc tgggcgattt
     2761 ccttcagcag cagaattggc atcaggctgg tataaaaact gccaggccca ataatgatga
     2821 gatccgcttc attgatagcg tgaaccgcct cacgcgttgc gggtacatta ggcgttaaca
     2881 ataactcttg aatcggcgta gttaactggt cgatattgac ctcgccgtaa acttcatgcc
     2941 cctgatcgtc aatcgccatc agatcaacag gatgctctga cattggaatc aaatgcgtat
     3001 ccactttcag cagattacga attaaattga tggcttccag aggccgcacg ctaaggtgat
     3061 ccagcgcctt taacatcaag tttccgagat tatgaccgga aagttcgcca ttgccgccaa
     3121 aacggtattc aaacatcgcg gaggcgacgt tcggttccgt tatcagctgg ttgaggcagt
     3181 tgcgcatatc gccccaagca atgccgcctt ctgaacggcg aatacgcccc gtcgagccac
     3241 cattatccgt ggtggtgacg atacccgtta aacgagaacc caaagacgaa agtgatgaga
     3301 gaacgcgtcc cagtccatgc cctccgccga gagcaacgac acgatcaaga tcagccagcg
     3361 tacgattgcg catatagttt cctggagtca gattatccgc gctacagtag cgcaaattgc
     3421 ggggaaacag caattaacct gccaatattg aggatacaaa ctattttctt ctatctctaa
     3481 aggacgatgc acgctatgcc tccctgatga tgtatatcaa agtaaaaccg ccattttccc
     3541 ttattctgta gcgaaatagc acgatcatga cgctatatac atgattacat agcgaaagtg
     3601 tggatggtaa aaatctcatt tacacgctag tatcggctta accactaaac actctagcct
     3661 ctgcacctgg gtcaactgat acggtgcttt ggccgtgaca atgctcgtaa agattgccac
     3721 cagggcgaag gaagaaatga cttcgcctcc cgtatctgga aaggtgtaca tggcttcaca
     3781 actgactgat gcatttgcgc gtaagtttta ctacttgcgc ctgtcgatta ccgatgtgtg
     3841 taactttcgt tgcacctact gcctgccgga tggctacaaa ccgagcggcg tcaccaataa
     3901 aggctttctt accgtcgatg aaattcgccg ggttacgcgc gccttcgcca gtctgggcac
     3961 cgaaaaagtg cgcctgacag gaggagagcc gtctttacgc cgcgacttta ccgatatcat
     4021 cgccgctgtg cgggaaaacg acgctatccg ccagattgcg gtcaccacca atggttaccg
     4081 tctggaacgc gatgtggcga actggcgcga tgcgggactt actggcatta acgtcagtgt
     4141 cgacagtttg gacgcccgcc agtttcacgc tattaccggg caggataaat tcaaccaggt
     4201 catggcaggg attgatgctg catttgaggc cggttttgag aaggtcaaag tcaataccgt
     4261 gctgatgcgt gatgttaatc atcaccagct cgacaccttt ctgaactgga tccagcatcg
     4321 ccctatccag ctgcgtttca tcgaactgat ggaaacgggc gagggcagcg agctcttccg
     4381 taagcatcac atctctggtc aggttctgcg tgacgagcta ctgcgtcgcg gctggatcca
     4441 ccaattacgt caacgcagcg acggtcccgc gcaagtcttt tgccatccag attacgccgg
     4501 agagattggc cttatcatgc cgtatgaaaa agacttctgc gccacttgca accgcctgcg
     4561 cgtttcctcc attggtaaac tccatctctg cctgtttggt gaaggcggcg ttaacctgcg
     4621 cgatctgctg gaaggcgata cccagcaaca ggcgctggaa gcgcgtattt cagcggcgct
     4681 gcgggagaag aaacagaccc atttcctgca tcaaaacaac accggtatta cgcaaaactt
     4741 atcgtacatt ggcggctaaa acgtcaaaag gagagatcag atgagtcagg taagcactga
     4801 atttatcccg acccgtattg ctattcttac ggtttctaat cgtcgcggtg aagaagacga
     4861 tacctccggt cactatctgc gcgattcggc gcaagaagcg ggccatcacg ttgtcgatga
     4921 agccattgtg aaagaaaacc gctacgctat tcgcgctcag gtatctgcgt ggatcgccag
     4981 cgacgatgta caagtggtat tgattacggg tggtactggc ctgacggaag gtgatcaggc
     5041 tcccgaagcg ctgctgccgt tgttcgaccg tgaggttgaa ggttttggtg aagtgttccg
     5101 tatgttgtcg tttgaagaga ttggcacttc cacgttgcaa tctcgtgcgg tagcgggcgt
     5161 cgctaacaaa acgctgattt tcgccatgcc gggttcgacc aaagcgtgcc gtaccgcatg
     5221 ggaaaatatc atcgcgccgc agctggatgc ccgtacgcgt ccgtgtaatt tccatccaca
     5281 tttgaagaaa taagtatgtc gcaactgacc catatcaacg ccgctggcga agcgcacatg
     5341 gtggatgtct ccgccaaagc ggaaaccgtg cgtgaagcgc gggcggaagc ctttgtcacc
     5401 atgcgcagcg agacgctggc gatgattatt gatggtcgcc accacaaagg cgacgtattt
     5461 gccactgcgc gtattgccgg tattcaggcg gcaaaacgca cttgggatct gatcccgctg
     5521 tgtcacccgc tgatgctcag caaagttgaa gtcaatttac aggccgagcc ggagcacaat
     5581 cgggtgcgta tagaaacctt atgccgcctg accgggaaaa ccggtgtcga aatggaagcg
     5641 ttaaccgcgg cctccgtggc ggcgctgacc atttatgaca tgtgcaaagc ggtgcaaaaa
     5701 gatatggtga ttggtccggt acgtttgctg gcgaagagcg gcggcaagtc gggtgacttt
     5761 aaggtggaag cggatgatta aagttctttt tttcgcccag gtgcgcgagt tggtgggaac
     5821 agatgcaacc gaagtggctg cggattaccc aaccgttgaa gcgttacgcc agcacctggc
     5881 tgcgcagagc gatcgctggg cgctggcgct ggaagatggc aaattgctgg ctgccgtcaa
     5941 ccagacgctg gtgagttttg accatccgct gactgacggc gacgaagtag ctttcttccc
     6001 gccggtaacc ggaggttaag atggcagaaa ccaaaattgt tgtcggcccg cagccgttca
     6061 gcgtaggaga agagtacccg tggctggcgg agcgtgacga agacggtgcg gtagtcacct
     6121 tcaccggaaa ggtgcgcaac cataacctgg gcgacagcgt caaagcatta accctcgaac
     6181 actatccggg gatgactgaa aaagcactgg cagaaattgt tgatgaagcg cgtaaccgct
     6241 ggccgctggg gcgcgtcacg gtgattcacc gcatcgggga attatggccg ggcgatgaaa
     6301 tcgtttttgt cggtgtcacc agtgcgcatc gcagcagtgc gtttgaagcc gggcagttta
     6361 ttatggatta tctcaaaacc cgcgcaccgt tctggaagcg cgaagccacg ccggaaggcg
     6421 accgctgggt tgaagcccgg gagagcgatc tgcaggcggc aaaacgctgg tagttttttg
     6481 ttagccggat aaggcaccgc tgcgtccggc aatttcaccg ctcagtacat gcgtttacgc
     6541 tgcgtcgatg cacagcctca tcattttgca gtatccttaa gatattcctt atatcttcag
     6601 gagatcgtca tggacagatt cccacgttct gattcaatcg tacaaccccg ggctggcttg
     6661 caaacttata tggcgcaagt ctatggctgg atgaccgttg gcttgttgct gaccgcattt
     6721 gttgcctggt atgcggctaa ttccgcggcc gtgatggagc tgttgttcac taaccgtgtc
     6781 tttttaatcg gtctgatcat cgcgcaatta gcattggtta ttgtgttatc agcgatgatt
     6841 caaaagctga gcgcaggtgt aacgacgatg ctctttatgc tttattcggc gctgacgggt
     6901 cttacgcttt ccagtatatt cattgtctat accgctgctt ctatcgccag tactttcgtc
     6961 gttactgccg ggatgttcgg cgcaatgagc ctgtacggtt acaccacgaa gcgcgattta
     7021 agtggctttg gcaatatgct gtttatggcg ttaatcggca ttgtgctggc atcgctggtc
     7081 aacttctggc tgaaaagcga agcattgatg tgggcagtta cctacatcgg cgtgattgtc
     7141 tttgtcggat tgacggcgta tgacactcag aaattgaaaa atatgggtga gcagattgat
     7201 acccgcgaca cgtcgaacct gcgcaaatat tccattcttg gtgcgttaac cttgtatctg
     7261 gacttcatca acctgttcct gatgttgtta cggatcttcg gcaaccgccg ttaattcttt
     7321 ttcaccatca ctatcccggc catgtgtcgg gatttttttc ttactaaaaa cgacacttaa
     7381 caagtaccag gtataaaata tgccttatga gcgagcggaa tcgttttccg tatccataaa
     7441 tacaaaagta ttttgtatgc gtggttttaa atttattgat ggtgaatatt aatgtttttt
     7501 ctaaggacaa ctaaaagaat ggaatcatac tcgcaaaaca gcaataaatt agattttcag
     7561 cacgaggcca ggatattaaa cggtatatgg ctcattaccg ctttaggttt ggtggcaacc
     7621 gcaggactag cctggggagc taagtatctc gaaattacgg caaccaaata tgattcacca
     7681 ccaatgtatg tcgccatagg gttattatta ctttgtatgt atggcttaag taaggatatc
     7741 aacaagataa atgccgccat cgcgggcgta atatatctgt ttttactctc tttggtggcg
     7801 attgtcgttg caagtttagt tcctgtatat gccattatca tcgtgttcag cactgcgggc
     7861 gcgatgtttt taatcagtat gctggccggt ttattattta atgttgatcc tggttctcac
     7921 cgttttatca ttatgatgac gttgacaggg ttggccctgg taatcatcgt gaatgcggca
     7981 ttaatgagtg aacggcccat ttgggtaata agttgcttaa tgattgtgtt atggccaggc
     8041 attatctcgc atggacgaaa taagctcctt gaacaggcag gaaaatgcca tagtgaagag
     8101 ttgtggagtc cggttcgttg cgcttttaca ggtgcattaa cactctatta ctattttatc
     8161 ggcttctttg ggatacttgc cgcgatagct ataacgcttg tctggcaaag gcatacgcgt
     8221 ttttttcatt agtcgtaaat gcagatacta aagaacttaa ctaaacttca catcgccgct
     8281 tcatttttcg cccgtagctt cttcgcttgg ctttccagta gcaggtaaca gatcagcgcc
     8341 agcagcagcg ggataaagta atacaggacg cgataagcga gcagggcggc gataatgctc
     8401 cctttcgagg tatgctcccc agccagtagt gcgataaaca ccgcttccag cacaccgatc
     8461 cccgccggaa tatgcacgat gacgccagca atactactaa ccagtaacac gcccagtaca
     8521 aagaaatagt tcacgctttg accaagtaac agccagataa tcgcccccat taccatccag
     8581 ttaacgctgg aaatcagcat ctgagcgagg gcgaatttcc atgaaggcag caccagcttt
     8641 tgccctttga tggtcatatg gcggtgcttc gcgaaagcgc aaaaccacaa ataaaccgcg
     8701 ataatcatca gtaagccaat gccgagaata cgcagcgtag tttgatcgac ataccagtaa
     8761 tccggcaact ccaccacgcc tgcggtaaag ataatccctg ccagtaaaat atagcccagc
     8821 cagttggtgg taatactgag cgaggaaatc cgcgtaatag tgctgcccgg taaccctagt
     8881 cgggagtaca aacgatagcg cataccaatg ccgccgaccc aggtactgag cgtcaggttg
     8941 aaggcgtagc agataaacga caccagcatc acctggcgct tcgccagttt gtgaccgcag
     9001 taaaagcggg cgagcaggtc atagcagccg taaatcagat agctgacgac caccagcccg
     9061 accgcactaa gcagcgcaac gcgattgtag tcgcggatga ccttccagac ctcttcccag
     9121 tccacttttt tggcgtagac caccagcaac acaatcaccg cgataaaaaa cagccaggtg
     9181 aggatcttct ttgctaagcg ccagcgcggg tgtgatttac tcatcagggg tttacccccg
     9241 tgttttcagt ttctacccga tcctgcgttt ccattgtcgg ttgtgcgggg ggatcaacct
     9301 gcgtcagacg tggtgtgtgt gccggaagcc agccaaccag cgccgggaag tggcgtaaaa
     9361 agtggaacgc cagcacgctt ttggtcaggt tccaccaggt gcgtttgggc agcatggttt
     9421 catccacctg ctgacaatct gcggcaataa tgccgttcag attatcgcgc agcgtctggt
     9481 taaaatgacg atcgtggatg atgacatttg cttcgagatt cagtgacaaa ctgagcggat
     9541 cgagattact ggaccctact gtcgcccagt gatcgaccat caatgccact ttgccgtgga
     9601 gcgggcggcg gcggtactca aaaacctgaa tgccgccttt aaccagatag ttatacagca
     9661 agcgcgcacc gactctgaca atcggcatat ccggttcgcc ctgaatgatc agtttgatcc
     9721 gcaccccgcg ccgtgccgct ttacgcaagg cgtgtaaaaa tcgatagccg gggaagaagt
     9781 aggcgttggc gataatcact tcccgctgcg cctgagtgag cattttcaga taatggcgtt
     9841 caatatcatc gcgatgttct tcgttatcgc gccagaccag caatacctgc gcttctcccg
     9901 gctggcggtt ctcttccgct ttgtgatgac gtcgccacca gcgtcgtgcc gcgctctgtc
     9961 caggcaggtt ttccagctca aactggagaa tatcttcgac aatcggccct tcaaggcgta
    10021 ccgcgtaatc ctgtttagcc tctggaccgt agctggacat atgctcggcg gagtaattca
    10081 gcccgccaat aaaggcgata cgcgcgtcga tcaccacaat tttgcgatgc atccggcgaa
    10141 acacattggt gcgcatacca aaaaggcgag ggcggggatc gtagtagcgg aacactacgc
    10201 cagctgccgt cagttcattg acaaactcat cgctgagatc cggcgaaccg tagccatcca
    10261 gcaagacttc cgctttaacc ccgcgttgcg ctgctgccag tagtgccgca tgcagctgtt
    10321 tgccgacgtt atcctcaaac cagataaacg tttcaagaat gatgcgttct tgtgcctcgc
    10381 caatcgcctt aaacaccgcg ggataatatt gctcgccgtt ttccagcaac tggatcttat
    10441 tgccttcgcg ccagctacat ttcataaatg aatctccgca cttaaagggg catgatcaga
    10501 aaggtgtcgc catgtccgca gcggcaacgc ggttggcgcg ctggcgctgg catttttgac
    10561 gtagatcctg tccagtcgta gtagaggaaa ttgcaccgga aacgtgcgcg ccgggcgtcc
    10621 gtgggcgcgg gtaaaaatct catccagtcc ggcctgcact tttaacggat gattagcttt
    10681 ttgccgccag tcattgaaat cacccgccac caataccggt tcgccgtccg gtagctcatt
    10741 cacccattcg gcgagcatcg caagctgcgc ctgacggtgc gcctcacgca ggcccagatg
    10801 tacgcacatc acatgaatcg cttttccggt catcggcggc acaatgcggc agtagagcac
    10861 gccgcgcttt tccgcaccat cgaccgaaac atcgcgattc tcataatgtt caatgggata
    10921 acgcgacagt acggcgttgc cgtgatgccc ttccgggtat acggcattgc gaccgtaggc
    10981 aaaatcgctc cacataatgt cggcgagaaa ctcgtagtgc gaggtatcgg gccagttttc
    11041 cacatgcagc ggatgaactt cgtgcgcgcc catcacttcc tgcaggcaaa caatatcggc
    11101 gctgacggta cgcacggcat cgcgaagttc cggcaaaatg aagcgtcggt taaacgcggt
    11161 aaagcctttg tgaatgttga tggtgagcac cttgaacgaa aattgttgtg tttgatcggg
    11221 cataattttc ctgtctttgc ctcttatctc attgaaatag tgtagtcggc gtcacaaaaa
    11281 ggtgcggtct tacggaattt tccgtaaagt tcggtactct gagtaagtag agataaattc
    11341 ttcaggagag aagccatgaa gtggcaacaa cgtgttcgtg tcgcaacggg tctaagttgc
    11401 tggcagatta tgttgcattt actggtagtg gcgctgctgg tggtgggctg gatgagtaag
    11461 actctggttc acgtcggcgt gggattatgc gcactgtatt gtgtcacggt agtgatgatg
    11521 ctggtgtttc agcgccaccc cgagcaacgc tggcgtgagg tggcagacgt gctggaagag
    11581 ctgaccacga cctggtattt tggcgcagcg ctgattgtgc tgtggctgtt gtcccgcgta
    11641 ctggaaaaca actttttgct ggcaattgca gggctggcga tccttgctgg cccggcggta
    11701 gtgtctttgc tggcgaaaga taagaagtta catcacctta cgtctaaaca tcgcgtacgc
    11761 cgctgaccct gtcgtggccg ttatcaccag tagcggccac aaactattcc acacaatatc
    11821 cagactcgca tccttcaaat aaatctgctt ggtaatgtcc gtaaagtggc gaatagggtt
    11881 aatccacgtc agattttgca gccacactgg catgttttcc accggcgaaa cgtaaccgga
    11941 aaggagaatg gcgggcatca taaaaacaaa cacgccgata aacgcctgct gttgtgtcga
    12001 gcagagtgat gaaatcaaca gaccgaatcc caccagcgat aaaccgtaaa tcaccatcgt
    12061 aaagtagaac agcgctagcg atccggcgaa ggggatttga tacgcccaga taccaatcgc
    12121 cagcacaatg gtggcctgga aggtggcgac aattaacgcc ggtacggctt tgccgatgaa
    12181 gatctgccag gtggtgagcg gcgaaaccag tagctgatcg agcgtacctt gttcacgttc
    12241 gcgggcgacg gaaagtgaag tgacgatcat tacgccgata gtggtgatca tggcgatcag
    12301 cgacggcacc acaaaccatt tgtagtcgag attcgggtta taccagttgc gtaccaccag
    12361 ctcgctgttg ttgggtttcg gttttccttc cagcagctcc tgctgataat ttttgacgat
    12421 ctgttgcagg tagttggcgg caatttgcgc actgttggag ttacgcccgt cgaggatcaa
    12481 ctgcaacggc gcggtctgga aggtatccag tttgcgcgag aagtcagccg ggaaacgcac
    12541 cagtagtaac gccttttgtg tgtcgatggt cgggcggatc tcctgtgggc ttttcagcag
    12601 cagcacatgt gtaaaggcgc tggcgcgggc aaaacgttga gtaagttcca ccgaatgctc
    12661 gccgttatct tcatcgtaga tggcgatggt ggcgttagtc acttccagcg tggcggcgaa
    12721 cgggaacagg atcacctgaa ttagcacggg taaaatcaga atcgcgcggg tttgtggttc
    12781 gcgcagcaac gactgcaact ctttgcggat taacgtccat aagcgatgaa acatgctctt
    12841 ctccctaatc cagccgacgt ttggttttca gccacgtcag gccgataaac atcaccgccg
    12901 aagcgatcaa aaacagcacg tttaccacca gcaccactgg aatattcccg gcgaggaaca
    12961 ggctttgcag ggtgctgacg aaataacgag cgggaataat gtacgtcacc gcgcggatca
    13021 ccgcgggcat actgtcgatc tgaaaaataa agccggaaag cataatcgac ggcagaaaag
    13081 cggcgttcag ggcgacctga gcggcattga actggttgcg ggtaatcgtg gaaatcagca
    13141 gccccattcc cagggtactg agtaaaaaca ggctggagat aaaaaacaga atcagcagcg
    13201 aaccgcgata cggcacgccg agaataaaca ctgacaccag catacacagc aacatcgcca
    13261 gcatcccgag aaagtaataa gggatcagct tacacagcag cagttccgtg cgggtaatct
    13321 ccgtagagag cagagcctcc atggtgccgc gttcccattc tcgcgccacc accagcgagg
    13381 tgagaatcgc gccgatgacc gtcatgataa tggtcaccgc acccgggata ataaagtgct
    13441 ggcttatcgc cgccgggtta aaccagtagc gggtttgtac atcaataagc ggttcaaaag
    13501 tctgcccgtt gtcctccgct cgctgcattt gccagatctg ccagatccct tcgacatacc
    13561 cctgcacaaa gttagcggta ttcggctcac tgccgtcggt gatcacctga atcggtgcgg
    13621 tggcgttggc gcgctccatc tgttcggcga aatccacggg aataaccacc agaccgcgaa
    13681 ttttccccga ctgcattttg gcgatcagtt cctgacggtt atcgctgatg gtggcgtcga
    13741 tgtagggcga accggtcatg gtgtgggtga aatccagcgc cgcttcgcta cgctgttcca
    13801 gcaaaatccc gacccgcagc ttgctggagt cgaggttaat gccgtaacca aaaataaaca
    13861 gcagtagcag tgggatcact accgcaatca gccagctact cggatcgcga acgatctgcc
    13921 gcgtctcttt gacgcacagc gcccgcacgc gatgccagga aaggatcggg ttactcattg
    13981 ctatgctcct tatcccagtc gtggatcaac tgaataaagg cttgctccat ggtgggatcg
    14041 ggttgctcat cgttagctga ctgtgctttc aaatcgtccg gcgtaccgct ggcgattaat
    14101 ttcccacggt acaccaggcc gatgcggtcg caatattccg cttcatccat aaagtgggtg
    14161 gtgaccatca ccgtgacgcc tttctctacc atgctgttga tgtgcagcca aaattcacgg
    14221 cgggtgaggg ggtcaacgcc ggaagtcggt tcgtcgagaa agagaatatc cggttcatgc
    14281 atcagcgaac aggccagcgc cagccgctgt ttaaaaccta atggcagttc atcggtggcg
    14341 tgtgaggcaa tacttttcag gccaaatgcc tcgctcatac gggagatttt ttcgttctgc
    14401 gcccgaccgc gtaagccata cacaccagag aaaaagcgta aattctgttc gaccgtcagg
    14461 ttgccgtaga gcgaaaattt ttgcgccata tagccgagat gctggcgcgc tttaccggaa
    14521 ctctctttca gatccatccc cagcaccagc gcctggccgg aagtcggcac cagcaaaccg
    14581 cacatcatct taaaggtggt cgatttaccc gcgccgtttg gccccagcaa accaaaaatc
    14641 tccccacgtt taacggcaaa gttgacgtga tcggtggcgg caaaatcacc aaatttcttg
    14701 gtcagttctt tcgcttcgat caccgtctcg ccgggtgtgc cttctaccgt atgcaatatt
    14761 gcacccagcg gcgattctga ggttccggca ccgcccagca aatcaataaa cgcatcttca
    14821 aaacgcggcg tagtttcgtt gatgttgatt tccggcatcc cgtcggcatg gcgaatatcg
    14881 tctggtgtgg cctctttttt gaggatcaga cgtaccgatt tcccctgaat catgccatcg
    14941 ctgacctgcg gcagtttcaa ggcgcgttgc aacagtttgc ggttgccctc gtgtggactg
    15001 gtcatcagaa agctgcgtcc ggccatggtt tgtgtcaggg cttttggttc tccctgatac
    15061 agcaactcgc cttcgttcat cagtaacacg tcacggcact gctcggcttc gtcgagatac
    15121 gaggtactcc agaggattaa catcccttcg cccgccagct catgcaccat ctgccacagt
    15181 tcgcgccgtg agatagggtc aacgccgacg ccgggttcat cgagcagcaa cactttcggt
    15241 tcgcccacca gggtacaggc caggccgagt ttttgtttca tcccaccgga gagcttgccc
    15301 gccaggcgtc cggtaaacgg cccaagagac gtgaactcca gcaggcgagc aaaagtttgc
    15361 ttacgtgcct cgccggtgac gctgcgcaaa tccgcgtaca gattgaggtt ctccatcacc
    15421 gtgagatctt catacagacc aaatttctgc ggcatatagc cgagcacggc gtgcagcgcg
    15481 ccgtcgtttt tgatcggatc aaagccaatc accgtggcac tgccgctgtc gggtttcagt
    15541 aatcccgcca acatccgcat cagtgtggtt ttacctgcac cgtccggccc caccaacccc
    15601 gtcacataac cggcgtgaat ggtacaatcg agcggcgcga cggcgggctt gtccatgccc
    15661 ggaaagcgtt tttccaggcc gttcagcgtg ataacggcat cattcatgtc ctgcctcgtc
    15721 accgaattgt accgtcactg gcattccctg gcgtaacgca tcatcggcgt cggtcaccac
    15781 aatacgcagg cgatagacga ggtcggtacg cagatccggt gtttcgacgg ttttcggggt
    15841 aaattcagca gtcggcgaaa cgaaaccaat ctgcccgtga tacggcttgt ccgggcgacc
    15901 atcggtataa agcagcactt tgcgccccgg ctgggcctgg tcaagattcc gttcatcaac
    15961 ataagcgcgc acccacaccg gacgcgttag tgaaacggta aacaccgtgc caccttcatt
    16021 gagaaccgtg cctggctcta ccgcgcgcgt taacagcgtg ccatcagacg gtgctatcaa
    16081 cgttgagtcc tgtaaattca gctccgcctg cgccagttgc gcctgcgcct gttcgaggct
    16141 ggctttcgcc tgagcgatgt cctgttcacg gttaccggaa cggtactgac gcaatttatc
    16201 ctgtgctgat ttcagcgttg cctgtgcctg gtcgcgcgag gagcgggcat tttccaggtc
    16261 atttgccgaa atggtgcggc ttttccacag tccttgctgg cggttataga agttctgcgc
    16321 atagtcatag gcggcttgcg cctgtttcac cgctgcggcg gcctgagcga tttcttcatc
    16381 gcgatacccg gcaagcatca gattatactg cgcctgtgcc accgagacgc ccgctttcgc
    16441 ctgcatcagg gcaatctcat acggcttgtg atccagttcg cccagcacct ggcccgcttt
    16501 gatagcatca ccttcgtcca ccgccagcga ttcaacgcgc cccccaacac ggaaactaag
    16561 attcaccgta cgaatatcca cgttgccata cagcgtcagg ccgttatcct ggcggctttg
    16621 ataccaccag tagcctccgg caaccacggc ggcaagtacc actaccgcca gtccgatcac
    16681 gacaggtttt ttcatcacta caaactcctt tgcgataatc cttgcaaaat caggtcgata
    16741 tgacaggtca ccgtctggtt gatcagttcg gtcttttctt catcgaacgc ggtccagccg
    16801 gtacgtaaca gaattgtttc tttgccaaga cggaacgcca gaatctcacc aatcaacgca
    16861 tgggtatgaa ggatcatgcg ggtgtcatta gcgtcgcagc cggtccaggc ggcaatcagg
    16921 cgtgtcaggt ggctgtgtag cggactaatc acctgttcgt gcaccaggtg gtaggctgcc
    16981 gtgggagaga gctgctcacg ggagataaac ttgctgaggt tgacggtgtc atcctgggtg
    17041 agcagcttaa tcatgttcct gcaggcgcga aggatcagtt cacggatggc agcccgatca
    17101 ggctgtggtt gggcgaacaa gcgttcggct tcctcggcat gcggacggaa ctgctcgcca
    17161 ataaaatcgg caatccactg ggcgcaggcg aggtacaaat cttctttcga accgaagtag
    17221 taggtgatgg cagcgatatt ctgcccggcc tgggcggcta tctcgcgggt ggtggcgttc
    17281 attccatatt caccaaactg cgccagtgcg gcagcaatca gctgtttttt cgcctgttca
    17341 cccttgattg tcatggcagg attattcatc gcacagtcca ttcttaatca aatgattgat
    17401 taagattatg actccatatg ggagttgtcc agtatggcta agaattttag ctacgccagt
    17461 cacagggata atttatgcgc tgcgtcacaa aaactgctac actccgctcc ctcatgacat
    17521 tgtggttttt gtcattttcc ttttcagtat ctccctgaaa actacaccgg taacggtcgg
    17581 ggcggttcgg agtagttatg tctttcgatt ctttgggttt aagccctgat atcctgcgcg
    17641 ccgttgccga gcagggttac cgtgaaccca cccctattca gcagcaggcg atccctgcgg
    17701 tgctggaagg ccgcgacctg atggctagcg cccagaccgg caccggcaaa acagcgggct
    17761 ttacgctgcc gctgttgcaa cacctgatca ctcgccagcc gcacgccaaa gggcgtcgtc
    17821 cggtacgtgc gctcattctt accccgaccc gtgaactggc ggcacagatt ggcgaaaacg
    17881 tccgtgatta cagcaaatac ctgaacattc gttcgctggt ggtgtttggc ggtgtcagca
    17941 ttaacccaca aatgatgaag ttgcgcggcg gcgttgatgt gctggtggca acgccgggac
    18001 gtttgctgga cctggaacat caaaatgcag tgaagctgga tcaggttgaa atcctcgtcc
    18061 tcgatgaagc tgaccgcatg ctcgacatgg gctttatcca cgatatccgt cgcgtgttaa
    18121 caaaactgcc agcgaaacgc cagaacctgc tgttctctgc gaccttctct gacgatatta
    18181 aagccctggc ggaaaaactg ttgcacaacc cgctggaaat cgaagtggca cgccgcaata
    18241 ccgcgtctga tcaggtgacc cagcacgttc actttgtcga taagaaacgc aaacgcgaat
    18301 tgctgtcgca catgattggt aaagggaact ggcagcaggt gctggtgttt acccgtacca
    18361 aacacggcgc taaccatctg gctgagcagc tcaataaaga tggcatccgt agtgtggcga
    18421 tccacgggaa taaatcgcaa ggtgcgcgta cccgtgcgct ggctgatttt aaatcgggtg
    18481 acattcgcgt actggtggcg accgatatcg ctgcgcgcgg tctggatatc gaagagttgc
    18541 cgcacgtcgt caaccatgaa ctgccaaacg taccggaaga ttatgtccac cgtatcgggc
    18601 gtaccggtcg tgcggctgct accggtgaag cgttgtcgct ggtgtgtgtt gatgaacaca
    18661 aactgctgcg tgatatcgaa aaactgctga aaaaagagat cccgcgcatt gcgattccgg
    18721 gctatgagcc ggacccgtca atcaaagccg aaccgatcca gaacggtcgc cagcaacgtg
    18781 gcggcatcag gctgatgaac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta
    18841 cgtgaactct gcaatatatt gaatttgcat gcttttgtag gccggataag gcgtttacgc
    18901 cgcatccggc atttcacaac aagcacttgt cagcaatttg agaacacggg aaaaaattta
    18961 tctgttttac cgcccataag ccaccgagac gttacttctc tatagccaat tgttcgcgca
    19021 actccattaa aaggtagccc agtcgattct tgcccttacc attaccaccg tctccccagt
    19081 aagcatcgtt ttccgtatgc tcaaccagtt ttgcgggcgc ggttgccagc aagagcacac
    19141 gcagttctgc atgctgttcg aatttagcgc gaagtgcttt acgcatcacc tgttctttaa
    19201 ccgactccca gtttttacga agtggcttgg aacgattacg ccccatgcga gcggcgacca
    19261 tgggggaaga aacccgacgg atctcttcgc ggtatttttc atcaaggaat ttttgtgcct
    19321 gaaaatagtg ttcggaggtg ggccaggtat tcccgtcaac cttgatgggc ctggcggcaa
    19381 agttagaaaa atcaccgtag tcatcgctgg tgctgtagaa atttatgatg gtgtcttgca
    19441 tgacgtgttg gatcctttgt gctcgaacgg gcattaaacc gcattatgtt ggtggttatt
    19501 gcgagccgct ttccagaaac agaaaaacca ttacccctga aaaccgaaaa atgccacaat
    19561 attggctgtt tatacagtat ttcaggtttt ctcatggcat taaccgccgc gcttaaagcg
    19621 caaattgccg cctggtataa ggcgcttcag gaacagatcc ccgactttat tccccgtgcg
    19681 ccgcagcggc agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga agggcggcat
    19741 ctggcgattg aagcccccac cggcgttggg aaaacgctct cctatttgat ccccggcatc
    19801 gccattgccc gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt ggcgttgcag
    19861 gatcagattt acagcaaaga tttaccgctg ctgaaaaaga tcattcccga tcttaaattc
    19921 actgccgctt tcgggcgtgg gcgctacgtt tgtccacgta atctgacggc gctcgccagc
    19981 actgaaccca cgcaacagga tctgctggcg tttcttgacg acgaactgac gccaaataat
    20041 caggaagagc aaaaacgttg tgcgaagctg aagggcgatc tcgacactta taaatgggat
    20101 ggtctgcgcg atcatacgga tatcgctata gatgatgatc tctggcgtcg tttgagtacc
    20161 gacaaagcca gctgcctcaa ccgtaactgt tactactatc gtgaatgccc gttttttgtt
    20221 actcgtcggg agattcagga agcggaagtg gtggtggcaa accatgcgct ggtgatggcg
    20281 gcgatggaaa gcgaagccgt actgcctgac ccgaaaaatc tgctgctggt gctggacgaa
    20341 ggtcatcacc tgccagatgt ggcccgggat gcgctggaga tgagcgctga aatcaccgcg
    20401 ccctggtatc gactacagct ggacttgttc acgaaactgg tcgctacctg catggagcag
    20461 tttcgcccga agaccatccc accgctggcg atccctgaac gtttgaatgc gcattgtgaa
    20521 gagttgtatg agcttatcgc ctcgttaaac aacattctca atctctacat gcctgccggg
    20581 caggaggcag agcaccgttt tgcgatgggc gaactgcctg atgaattgct ggagatctgc
    20641 cagcggctgg caaaactcac cgagatgctg cgtggtctgg cggagttatt tcttaacgat
    20701 ttaagtgaga aaaccggcag ccacgatatt gtgcgtctgc atcggttgat tttacagatg
    20761 aaccgcgcgc tggggatgtt tgaggtgcaa agcaaactct ggcggctggc gtctctggcg
    20821 caatcttccg gtgcgccggt gaccaaatgg gcgacgcggg aagagcgcga agggcagcta
    20881 cacctctggt ttcactgcgt gggaatacgt gtcagcgatc agctggaaag gctgctgtgg
    20941 cgcagtattc cgcacattat tgtcacctcc gcaaccttgc gttcgctgaa tagtttttcg
    21001 cgtttgcagg agatgagtgg tctgaaagag aaagcgggcg accgttttgt ggcgctggat
    21061 tcccccttta accactgcga acagggcaaa attgttattc cccggatgcg ctttgagcct
    21121 tccatcgaca acgaagagca acacattgcc gaaatggcgg cctttttccg tgaacaggtg
    21181 gagagcaaaa aatatctcgg tatgttggta ctgtttgcca gcggacgggc gatgcagcgc
    21241 tttctcgact atgtgacgga tttacgcctg atgttgctgg tgcagggcga tcagccgcgt
    21301 tatcgcttag ttgaactgca ccgcaaacgc gttgccaacg gtgagcgtag cgtgctggtg
    21361 ggcttacagt catttgccga agggcttgat ttgaaaggtg atctgctcag ccaggtgcat
    21421 atccacaaaa tcgcttttcc gcccatcgac agcccggtgg tgatcaccga aggggaatgg
    21481 ctgaaaagcc tcaaccgcta tccgtttgag gtgcaaagcc tgccgagcgc ctcgtttaac
    21541 ctgattcagc aggttgggcg actgattcga agccacggtt gctggggcga agtggttatc
    21601 tacgacaaac gcttgctgac aaaaaattat ggcaagcgac tactggatgc attaccggta
    21661 tttccgatag agcaaccgga agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa
    21721 tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta aatggactat
    21781 cgcaaaatca ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat
    21841 actgcgcgcg gtctgtatgc tcatatgctc aatggtgaag tccctgacct tgagttgggc
    21901 ggcgtattga ttgcgctgcg tatcaaaggg gaaggggaag cagagatgct cggcttttac
    21961 gaagccatgc aaaatcacac catcaagctg acgccgcctg caggcaagcc gatgccgatt
    22021 gtcatcccca gttacaacgg cgcgcgtaaa caggctaacc tgacgccatt attggcgatt
    22081 ctgctgcata aactcggttt tcctgtggtg gttcacggag ttagcgaaga tccaactcgc
    22141 gtgctgacag aaaccatttt cgaactgatg ggtattacac caacgttgca cggcgggcag
    22201 gcgcaggcga agctcgacga acatcaaccg gtgtttatgc ctgtcggcgc gttttgcccg
    22261 ccgctggaaa aacaactggc gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc
    22321 ctggcgaaac tggcgacgcc atttgccgaa ggtgaggcac tgtgtctttc cagcgtttcg
    22381 catccggaat acattggacg cgtcgcgaag ttctttagcg atatcggcgg gtgggcgtta
    22441 ctaatgcatg gtactgaagg cgaagtgtat gctaatccgc agcgctgtcc gcagatcaat
    22501 ctcattgacc gtgaagggat gcgggtgctg tatgaaaaac aggaccctgc tggtagcgag
    22561 ttactgccac aagcaaaaga tccggaaacc acggcgcagt ggactgagcg ttgcctggct
    22621 ggcagcgaag cgattcccga atcgctgaaa atccagatgg cttgctgcct ggtggctacg
    22681 ggtgaagcgg caactatcag cgatggcctg gcgcgcgtta atcaggcatt ttaattttta
    22741 tctcagccgg atgagccacg tcatccggct tttaccccac cgcaatatga aattcctgca
    22801 tctttattga ccttcccgcg cccggcgtgc agcataaaaa aacaacaaac acataacata
    22861 aacaggagtt aaccatggaa agtggtcatc gctttgatgc tcagacgctg cacagtttta
    22921 ttcaggctgt atttcgtcag atgggtagcg aggaacaaga agcgaaatta gttgccgatc
    22981 atttaatcgc ggcaaacctg gcagggcatg attctcatgg tattggcatg atcccaagct
    23041 atgtgcgttc ctggagtcag gggcatctgc aaattaacca tcatgccaaa atcgttaaag
    23101 aggcgggggc ggcagtcacg ctcgatggcg atcgcgcatt cggtcaggtc gcggcacacg
    23161 aagcgatggc gctggggatt gagaaagcgc atcagcacgg tattgctgcc gtggcgctac
    23221 ataactcgca tcatatcggc cgtatcggtt actgggcgga gcagtgttca gcggcggggt
    23281 ttgtctctat ccactttgtt agcgtggtcg gtattccaat ggtcgcgccg ttccacggtc
    23341 gcgacagccg ctttggcacc aatcccttct gtgtggtttt ccctcgtaaa gataatttcc
    23401 cgctgttgct tgattacgcc accagcgcca ttgcatttgg caaaacccgc gtcgcctggc
    23461 ataaaggcgt ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg ccgacgacca
    23521 atccggcggt aatgcaggag tcgccgttgg ggtcgctgtt gacctttgcc gaacataaag
    23581 gctacgccct tgcagcgatg tgtgaaattc ttggcggggc gctttctggc ggtaaaacga
    23641 cgcatcagga aacgttgcaa accagccccg atgccattct taactgcatg accactatca
    23701 tcatcaaccc ggaactgttc ggcgcgccgg attgtaacgc gcagaccgaa gcctttgccg
    23761 agtgggtgaa agcctcgccg catgatgacg ataagccgat tttgctaccg ggcgagtggg
    23821 aagtgaacac gcgtcgcgaa cggcaggagc aggggattcc attggatgcg ggaagctggc
    23881 aggccatttg tgatgcggcg cggcagattg gtatgtcgga agagacgttg caggctttct
    23941 gtcagcagtt agccagctaa aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa
    24001 cataggatca atgagggtta gagcatatgc gtctgtcggc aaacagacag ggaaatactt
    24061 gtgctggacg tagcgtaaac gcctgatccg tccaacggtt cggattttgt aggcctgata
    24121 agacgcggta gcgtcgcatc aggcatctgt tgccggatgc ggcataaacg ccttatccgt
    24181 cctacgaatt acttataaat taccgcagta ccgcttaatt tattgttgtt ggtggcggaa
    24241 gtgatgctgt atccactggc accggctgcc gccgctttct cagccagttt cgcttccagg
    24301 gcatcgaggg tggatgcgcc atcggcagaa accacgccga ttttattcat gttctgtgcc
    24361 tgggatgccg ttaccggttc cgtcgcgaac acgccaaatg acagagttga aagagccata
    24421 gcagcaacaa cagtattgat agttttcata attaatctct cgcaggtgat ttttgtataa
    24481 gtgacgttgt ttcgtcgatg tgatgagtat cacgtttttt tacgagagat aaaatcgaag
    24541 agaattgacc gccttgttca aataaattga ttgatatcta acatactgat aaaccattta
    24601 tttgttgtac ttatggactt gttttatgcc gcgtggcagt cacagcagcg ttaaaggcac
    24661 ggaatgccac gcaaaataca aattatactc gctatgcatc tcgccagttg tagttaacgt
    24721 aactgtctgt ctttcgaacc tctgcgatta tatcctgtat acgctggttt ttgtaaatct
    24781 ttctcctgct gacaatgaat acataagcgc acgccaggaa tggcttcccg acgggcttgc
    24841 gggatggggg caccgcactc ttcacattca tacaggcttt cgccacgcgg aatttcaccc
    24901 cgagcgcggg caatcgcatc ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg
    24961 ttagcccaag cggatgccat atcgtcctcc ccatatcaat gcttgtacag ttaagtgtag
    25021 ctaatatagg gacgaacctg ggcagttcaa gcgtcagatc tccgaccatt cccgcagcag
    25081 attatgataa agattgagca gcgacaggat ctcttcattt tcaccatagc ggcttttcag
    25141 cgactgaata tttttgtcca gttcaaacag catggcgcgc tttttatcat cgcggatcat
    25201 cgactggatc cacataaatg atgccactcg tacgccgcgg gttacgggtg tcacgcaatg
    25261 caggctgctg gagggataca acaccagatc acctgccggg agttttaccc gatgttgtcc
    25321 aaaggtgtca ttaacgacca gttcgccgcc gtcgtagctt tgtggatcgc ttaaaaacag
    25381 cgtggcagaa aggtcagtgc gcatccagcc gttttgtgga tggctgcgta ctgcgccatc
    25441 cacatggaaa ccataggttt cattgttctg atagcgatta aacagcggcg tggaaagggt
    25501 acgcggcaag gcagcggcaa agaacaatgc atgttggtta accgcgttca gcacctcatt
    25561 ttgcagtgcg gcgtataacg cgctgcgggt gtctacttgc tgattgttct taacttgtgc
    25621 accttgtgct ccggtggtga cgcgtccatc cacccattcg gcttgttcca gttgttcgcg
    25681 aaaatgggcg acgtcctgtg gcgataacac gccgggaatg tggtacatca tacaagttct
    25741 ccaaaaagtg gggcctgcgc cccacatctg aatcagaaat gcatattggc tgtgagcaag
    25801 aaggttcttg gctcgcccgg gtgataacgg tagccgctct tgttgattga ggcgacgtaa
    25861 tcggtatcaa acaggttgta aacgtttagc tggaagtcga gattgcgatt aacccgatac
    25921 cccagtttgg catcggcgac ccagtaacct tctgtaaatg ctggcgttcc caccgcgccg
    25981 tctgaacctt tatgtatact gccgatatag cgtgcgcccg cgccaacaga gatatcatcg
    26041 gttgcctgat attggctcca taaggtgaag gcgtgctccg gggtatacgg cagcgatgag
    26101 gaaccatcct gggcaacatc tttgccgttt ttgatggttg ctttttgctg ggtatagccg
    26161 ccaatcacct gccacgcggg agtgatattc cctgccacgg atatctcata gccttcgacg
    26221 cgtttcttac cgtattgcga gtaagtcccg tcatcatttt gctcaacttc attttcgata
    26281 tcagtacgga acagcgcggc ggtgagcaac aggcgtttat ccagaacctg ccatttggtg
    26341 ccaatctcgc tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc actgttacca
    26401 ctgccagact gcgcaagggc gaagttgttg ccgcccggag gctgctggga aacggcatag
    26461 ttaatataga cattgccgtt ttccgtcagg tggtacagcg ccccggcttt ccagttcacc
    26521 agattgcccg acttggcggt gtcgacggtg gtgactggag aaccttttgc cacaccagct
    26581 gggcaggtga tggcaccgcg tccgctgccg ccgcaggcgg tggcactgtc atattcagta
    26641 tgataattat ccagacggat cccgccgttc agctcaaaat cacgggtgat ttgcagcgta
    26701 tcgaaggcgt aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc gttgcgcgtc
    26761 aggccgccgg gatgaattct gctgtcagga tgataaatat ttaccgccgg taacgtcacc
    26821 ggattaacgc catagttagt ctgcgtttca cgggtaaatt ccacgccggt actgacatca
    26881 tgaccgatag aaccggtata gaacgtcgag gtcaggttgg tctggttggt gagaatttta
    26941 ttactcacat ctttggtatt cgccgtgcgt gactaggtcc agctattcac atcgctggtg
    27001 ggctgagtaa tattcgacgc cccgcccata atcgccgtca tcaggtaatc ctgctttacg
    27061 cgcgaccaac gggtagtatt gcgaatggtg gtgttatcgt tgatgtcgtg ctcaaaacgc
    27121 atggtggcgg tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta aaagttatga
    27181 gtatcaactt ttccggaatg attcagggcc gccgctcccg cagatggggc agaatagccc
    27241 ggcaaaccga tggtcggaat gccgccgtct ggcgtgttgt gctgagtgac atgcagataa
    27301 ttaagataca aacgatttgc tgtaccaagg ccaaaagcga cagaaggggc gacgccgtaa
    27361 cgctcatttt tgactttgtc gcgtccggca tcgtgggttt tttcgcccat cacattcagg
    27421 cgcaccgcgg tagtatcacc aatgacctga ttgacgtcca gcgtgccgcg gcggaaccag
    27481 gcgctgccaa tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg cttgctgatc
    27541 atattgatcg agcctgtcgg tgcgctgcgc ccgtagtcgg tgccggacgg ccctttaatc
    27601 acttcgacct gctcggtatt gaaggtgtcg cgcgagacgc tgccgatatc gcgaatacca
    27661 tcaatataaa tactgttaga ggtatcggca ccacgcatat aaatggcgtc gccagtggtg
    27721 gagttaccgt tctcacccgc aaaaaacgca cccacgccgg
//
LOCUS       DAEBXD010000049        27590 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEBXD010000049 DAEBXD010000000
VERSION     DAEBXD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27590
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.049"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KJL94_002214"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KJL94_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBD4410554.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KJL94_002215"
     CDS             complement(1614..1937)
                     /locus_tag="KJL94_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBD4410555.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KJL94_002216"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KJL94_002216"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBD4410556.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KJL94_002217"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KJL94_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBD4410557.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSYARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KJL94_002218"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KJL94_002218"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBD4410558.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KJL94_002219"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KJL94_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBD4410559.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KJL94_002220"
     CDS             7537..8574
                     /locus_tag="KJL94_002220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD4410560.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KJL94_002221"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KJL94_002221"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBD4410561.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KJL94_002222"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KJL94_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBD4410562.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KJL94_002223"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KJL94_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBD4410563.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11615
                     /locus_tag="KJL94_002224"
                     /pseudo
     CDS             10745..11615
                     /locus_tag="KJL94_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(11650..12984)
                     /gene="srmB"
                     /locus_tag="KJL94_002225"
     CDS             complement(11650..12984)
                     /gene="srmB"
                     /locus_tag="KJL94_002225"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBD4410564.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13116..13853
                     /gene="trmN"
                     /locus_tag="KJL94_002226"
     CDS             13116..13853
                     /gene="trmN"
                     /locus_tag="KJL94_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBD4410565.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13838..15460)
                     /gene="nadB"
                     /locus_tag="KJL94_002227"
                     /pseudo
     CDS             complement(13838..15460)
                     /gene="nadB"
                     /locus_tag="KJL94_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15716..15871
                     /gene="rseD"
                     /locus_tag="KJL94_002228"
     CDS             15716..15871
                     /gene="rseD"
                     /locus_tag="KJL94_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBD4410566.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15868..16443
                     /gene="rpoE"
                     /locus_tag="KJL94_002229"
     CDS             15868..16443
                     /gene="rpoE"
                     /locus_tag="KJL94_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBD4410567.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16476..17126
                     /gene="rseA"
                     /locus_tag="KJL94_002230"
     CDS             16476..17126
                     /gene="rseA"
                     /locus_tag="KJL94_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBD4410568.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17126..18082
                     /gene="rseB"
                     /locus_tag="KJL94_002231"
     CDS             17126..18082
                     /gene="rseB"
                     /locus_tag="KJL94_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBD4410569.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18079..18558
                     /gene="rseC"
                     /locus_tag="KJL94_002232"
     CDS             18079..18558
                     /gene="rseC"
                     /locus_tag="KJL94_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBD4410570.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18756..20555
                     /gene="lepA"
                     /locus_tag="KJL94_002233"
     CDS             18756..20555
                     /gene="lepA"
                     /locus_tag="KJL94_002233"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBD4410571.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20571..21545
                     /gene="lepB"
                     /locus_tag="KJL94_002234"
     CDS             20571..21545
                     /gene="lepB"
                     /locus_tag="KJL94_002234"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBD4410572.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21817..22497
                     /gene="rnc"
                     /locus_tag="KJL94_002235"
     CDS             21817..22497
                     /gene="rnc"
                     /locus_tag="KJL94_002235"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBD4410573.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22494..23399
                     /gene="era"
                     /locus_tag="KJL94_002236"
     CDS             22494..23399
                     /gene="era"
                     /locus_tag="KJL94_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBD4410574.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23411..24139
                     /gene="recO"
                     /locus_tag="KJL94_002237"
     CDS             23411..24139
                     /gene="recO"
                     /locus_tag="KJL94_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBD4410575.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24151..24882
                     /gene="pdxJ"
                     /locus_tag="KJL94_002238"
     CDS             24151..24882
                     /gene="pdxJ"
                     /locus_tag="KJL94_002238"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBD4410576.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24882..25262
                     /locus_tag="KJL94_002239"
     CDS             24882..25262
                     /locus_tag="KJL94_002239"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBD4410577.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25682..25762
                     /gene="shoB"
                     /locus_tag="KJL94_002240"
     CDS             25682..25762
                     /gene="shoB"
                     /locus_tag="KJL94_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBD4410578.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25956..26216)
                     /gene="yfhL"
                     /locus_tag="KJL94_002241"
     CDS             complement(25956..26216)
                     /gene="yfhL"
                     /locus_tag="KJL94_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBD4410579.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26431..27527
                     /locus_tag="KJL94_002242"
                     /pseudo
     CDS             26431..27527
                     /locus_tag="KJL94_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6805 a         6865 c         7230 g         6690 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtagctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgttgctgcc gcacatttat gaacttaccc gagtgatgga tacgcttcgt gaggctgcca
    10981 aaaaggagtc agatccggac ggggaactac gcgttgtttc gggcgaaacg ttgctctctt
    11041 accgaatgcc gcaggtgttg cagcgttttc ggcaacgcgc accaaaagtg cgtttatcgt
    11101 tgcaggcgct gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg
    11161 gcgtctttta tcgcgtaggg aatgatgatg ccctgaaccg acgagagttg ggtgaacaat
    11221 cgctggtgct ggtggcttca ccacaaattg cagatgtcga ttttactgag ccgggaagac
    11281 ataacgcctg tagctttatt atcaacgaac cgcaatgtgt cttccggcag atatttgaga
    11341 gcacgctgcg acagcggcgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa
    11401 gcatcaagcg ttgtgtcgcg gcgaatatcg gtgtcagtta cttgccccac tttgcggtgg
    11461 aaaaagagat tgagagcgga gaattaattg aattgccttt tggcgaacag tcgcagacca
    11521 ttacggcaat gtgcgcacat tatgctggaa aagcggttag cccggcgatg cacattttta
    11581 ttcagtgtgt tgaagagtgt tttgtggttg cgtaaaaaaa caatgccggg cttaaacccg
    11641 gcattgagat tactcttctg tcgtttgcgg tggcacgccc gttccgctcg gtttacggcg
    11701 cttaccaata tttttggtgt cgcgatggcg ttttttcacc cgcggcttct ctttctcttt
    11761 agcttttttc ttctcggcgc gttttgccag tactttcttc gatggcttgc cggtctgctt
    11821 ctcgcttggc gcacgcgttt tcgggcgtaa ctcatcaata acgcgagctt taatcggctc
    11881 ttcaatgtaa cggccaacct tgcccagcag caaatggtca tgggcttcca ccagcgaaat
    11941 tgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata cgatgcaaat aagtatcgcc
    12001 actgcgcggc atatcgaagt taaagacgtg gctgacgtca ggaatgtcga taccgcgcgc
    12061 ggcaacatcg gtggcaacca gtacgtttac gcggccttcg gtcaggcgtt taattgcttc
    12121 gttacgcttg ccttgtacca tctcaccttc gagatagcag ttgttgatgc ccgcttcgcg
    12181 cagccagttt gccagctcat gcacacgttc acgcttacgc acaaacacga ttgagcgagt
    12241 cgcttcaggc tgttttaaca ggtgcaccag caatgcggtt ttatgttcca ggtcatcggc
    12301 gcggtaatac cactgatgaa tttttttgcg ctcacgggtg gacggattgg cagaaacttc
    12361 caccggatct tccagcagac gctcggcaaa gtcctgaatc gcatcgcctt ccagcgttgc
    12421 cgaaaagagc agggtctgtt tacgccagcg agtttcgcca gcaatatgtt cgatatcctg
    12481 agcgaagccc atatccagca tacggtctgc ttcgtcgagg atcagcgttt caaccgcgcg
    12541 gcaatcgaag ttctcttctt ttatgtattg cagcagacgt ccggtcgtgg cgaccacgat
    12601 gtcctgattt tcgctgaaca cttccgcgtg gttcatatag gctacgccgc cggtgatggt
    12661 ggcgatatcc agatgcgtat gtttcgccag ttcgcgggca tgatcggcca cctgcatcgc
    12721 cagctcgcga gttggggtga ggatcaaaat acgcggcgga ccggatttct tacgtgggaa
    12781 atcgagcagg tgctgcaacg ctggcagcag atacgccgcc gttttaccgg tgcctgtcgg
    12841 cgcagaaccg agtacatcac ggccatcgag cgcaggcgga atggcggcag cctgaatggc
    12901 ggtcgggcga gtgaaacctt tatcctggag ggcttccagc aggctttcgt cgagttcaag
    12961 ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg gggcgctgat tatagacgtt
    13021 acggctgcaa tcttcatctg tttgtatgga tatcgcttct cgggtattgt tctcacccgg
    13081 tgctatcgcc gtttttcctg caaggtttta ttttcatgtc acagtctaca tccgtgcttc
    13141 gtcgtaatgg atttactttt aaacagtttt ttgttgctca cgatcgctgt gcgatgaaag
    13201 tgggaacgga tggcattttg ctgggcgcat gggcaccggt ggctggggtg aaacgttgcc
    13261 ttgatatcgg cgcgggtagc gggttgctgg cactgatgct ggcgcagcga accagtgaca
    13321 gcgtgataat tgatgccgtt gagctggaaa gtgaagctgc cgcgcaggcg caggaaaata
    13381 tcaaccagtc cccgtgggca gagcggatta acgtccatac ggcggatatt cagcagtgga
    13441 tcacacagca gacagtacgc ttcgatttaa tcatcagtaa cccaccttac tatcagcagg
    13501 gagtagagtg cgcgacacct caacgggaac aggctcgcta taccactacg cttgatcacc
    13561 catcgctgct gacctgtgcg gcggagtgca ttaccgaaga gggatttttc tgcgtggtgc
    13621 tgccagagca gattggtaat ggttttactg aactggcatt aagcatgggc tggcatttac
    13681 gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc gcatcgggtg ctgctggcat
    13741 tctccccgca ggcgggagaa tgctttagcg atcgcttagt cattcgtggg ccagaccaga
    13801 actattccga agcgtatacg gcgctgaccc aggcttttta tctgtttatg taatgattgc
    13861 cgggggaaag gatcgatgga ccggaatgcg tgagcagttc cgggtaatcc agcgtaaaat
    13921 gcagcccccg gctttctttg cgcatcatcg cgcagcgaac aatcaactcg gcaacctgta
    13981 ccagattacg cagctccagc aaattatttg agacgcggaa atgggcgtaa tattcgtcta
    14041 tttcttgttg gagcatggtt atccgccgca gggcgcgttc caggcgcttc gttgtgcgca
    14101 caatgccaac gtaatcccac ataaacagcc gtagctcgtg ccagttatgc tgaattacta
    14161 cccgttcgtc agggttctca acgcggcttt catcccacgg cggtaacgta ctgacgccgt
    14221 gggcataagg catacgtctg gtgatatctt ctgccgccga ccagccatag accagacact
    14281 ccagcaatga attcgaggcc atgcggttag cgccgtgtaa gccggtataa ctcacctcgc
    14341 caatggcata caagccctcg acgtccgtac gcccatgatc atcaaccatt acaccacctc
    14401 aggtatagtg tgcagcaggc acaatcggta ccggttcttg tgtgagatca atccccagcc
    14461 cgagcaattt ttcataaatc atcgggaaat gctggcgaat aaaatcggcg ggcttatggc
    14521 tgatgtcgag gaacatacaa tctgcgccga ggcgtttcat ttcatggtca atggcgcggg
    14581 cgacaatatc gcgcggggcc agttcgccgc gctcatcaaa atcgggcata aaacgcgtgc
    14641 catccgggcg cttgagataa gcgccttcgc cgcgcagtgc ttccgttaac aggaaattgc
    14701 gtgcctgtgg gtgatacagt gcggtagggt ggaactgatt aaattcgaga ttggcaaccc
    14761 gacagcccgc gcgccacgcc atagcaatgc catcgccaga agaaatatcc ggattggtgg
    14821 tgtactgata caccttcgac gcaccgccgg ttgccagtac cactgctttt gcgtggcagg
    14881 tttccaccgt ttctttattt cggttccata cccacgcgcc tacaacccgt cgcgtgcccg
    14941 gcaggccaat tttgtcagag acaatcagat caactgcgtt gctgcgctcc agcacacgaa
    15001 tattcggatg gttctgcgcc ttgctcacca gcgtggattg tacttctcta ccggtggcgt
    15061 cggcggcatg aagaatacga cggtgactat gtccaccttc acgggtcaga tggtagcttt
    15121 cttcgccatt cggttgaacg tgggtatcaa acaaaacccc ctggtcaatt aaccatggca
    15181 cacaggaacg agcattgctg gcgacaaatt caactgcatg gtgatcgcaa ataccagccc
    15241 cggcaatcaa tgtgtcttcc acatgcgagt caatgctgtc agtttcatca aacacggcgg
    15301 caataccgcc ctgggcataa aatgttgaac cttccgttac cgggccttta cttagaacga
    15361 tgacctgatg ctggtcagcc aggcgtagcg ccagtgaaag tccggctgcg ccgctaccaa
    15421 taatcaacac gtcacatgaa tgttcaggga gagtattcat tttctttgtt taatttacta
    15481 aacattgttt ggtcagcata gcatcatgtt gtgcgaataa acacctgcta ttttaatatt
    15541 tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa aaagagaagt tactggctgg
    15601 tggaggatta ggtggtgaaa taaaaaggcc gttgggttac tcttcaggca gttaaatggg
    15661 catttctaca cagataatgc gatgttcaga ttctgtagac ttataatgat agataatgat
    15721 ccgtctacag catgacaaac aaaaacagat gcgttacgga actttacaaa aacgagacac
    15781 tctaaccctt tgcttgctca aattgcagct aatggagtgg cgtttcgata gcgcgtggaa
    15841 atttggtttg gggagacttt acctcggatg agcgagcagt taacggacca ggtcctggtt
    15901 gaacgggtcc agaagggaga tcagaaagcc tttaacttac tggtagtgcg ctatcagcat
    15961 aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg atgttcccga tgtggtacaa
    16021 gaagctttta ttaaagccta tcgtgcgctg gattcgttcc ggggagatag cgctttttat
    16081 acatggctgt atcggattgc tgtaaataca gcgaaaaatt acctggttgc tcaggggcgt
    16141 cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa acttcgaaag tggcggcgcg
    16201 ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag aagaactgag acagatagtt
    16261 ttccgaacta ttgagtccct cccggaagat ttgcgcatgg caataacctt gcgggagctg
    16321 gatggcctga gctatgaaga gatagccgct atcatggatt gtccggtagg tacggtgcgt
    16381 tcacgtatct tccgagcgag ggaagctatt gataacaaag ttcaaccgct tatcaggcgt
    16441 tgacgatagc gggatactgg ataagggtat taggcatgca gaaagaacaa ctttccgctt
    16501 taatggatgg cgaaacgctg gatagtgagc tgcttaacga actggctcat aacccagaaa
    16561 tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc aatgcggggt gatactcccg
    16621 aggtgctcca tttcgatatc tcttcacgcg tgatggccgc cattgaagaa gagccagtac
    16681 gtcaatcggc gacattgatc ccggaagccc agcctgcgcc gcatcaatgg cagaaaatgc
    16741 cattctggca gaaagtacgt ccgtgggcgg cacagcttac ccaaatgggc gtagccgcat
    16801 gcgtatcgct tgcagttatc gttggcgtcc agcactataa tggacaatct gaaacgtccc
    16861 agcagcccga aacgccggta tttaatacac tgccgatgat gggtaaagcc agcccggtaa
    16921 gcctgggagt accttctgaa gcgaccgcaa acaatggtca acagcagcag gtacaggagc
    16981 agcgtcgtcg cattaatgca atgttgcagg attacgaact gcaacgccga ctccactctg
    17041 aacagcttca gtttgagcag gcgcaaaccc agcaagccgc tgtacaggtg ccaggaattc
    17101 aaactttagg aacgcaatcg cagtaatgaa gcaactttgg tttgccatgt cattagtgac
    17161 aggtagcctg ttattctctg ctaacgcctc ggccactccc gcgtccgggg cgttattaca
    17221 gcagatgaac ctggccagtc agtcactgaa ttacgagctg tcattcatca gcatcaataa
    17281 acagggtgtt gagtctctgc gttatcgaca tgcacgcctc gataaccgtc ctcttgcaca
    17341 attgttgcaa atggatggcc cgcgccggga agtggtacag cgcggcaatg aaatcagcta
    17401 ttttgaaccg ggacttgaac cgttcacgct taatggcgat tacattgttg attctctgcc
    17461 atcgcttatc tataccgatt tcaaacgcct ttctccttac tacgacttta tctccgtcgg
    17521 gcgcacgcgt attgctgatc gtctttgcga agtcattcgc gtggttgccc gagatggtac
    17581 acgctacagc tacatcgtgt ggatggacac cgaatcgaaa ttaccgatgc gggttgatct
    17641 ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg attgctttta acgtcaatca
    17701 ggatatcagc agcagtatgc agacgctggc gaaggcaaat ttgccgccgt tgctttctgt
    17761 tcctgtaggt gaaaaagcta aattcagctg gacgccaacc tggttgccac agggttttag
    17821 cgaagtttcc agtagtcgac gtccgctacc gacgatggac aacatgccta tcgaatcacg
    17881 tctctattcc gacggattat tcagcttctc ggtaaacgtt aaccgcgcta cgccatcgag
    17941 caccgatcag atgttgcgca ccggacgcag aaccgtcagt acaagcgtac gtgataacgc
    18001 cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg aaacgcattg ccgagaatat
    18061 taagttcggg gcagcgcaat gatcaaagag tgggctaccg tcgtctcctg gcaaaacggg
    18121 caggcgctgg tcagttgtga tgttaaagct tcatgcagca gctgcgcttc acgcgccggt
    18181 tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca cgcataccat tgtcgtaccc
    18241 tgtgatgagc cgttagtgcc ggggcaaaaa gtggaattgg ggatcgccga aggcagcctg
    18301 cttagctccg cattactggt ttatatgtcg ccgctggtgg gattattcct catcgcttcg
    18361 ctatttcagc tactctttgc ttctgacgtc gcagcattat gtggtgcggt cctcggcggt
    18421 attggcgggt tcctgattgc gcgcggctac tcgcgtaagt ttgctgcccg ggcagaatgg
    18481 caaccgatca tcttaagcgt ggcattgccg cctggccttg tgcgatttga aacatcttcg
    18541 gaagacgcga gccagtgatt tcactggctt tgttgcattt tgcataacaa atgcgaggac
    18601 gtttccagat accacaatcc atacggtatg aacatttcct cgcctcaatg ttgtagtgta
    18661 gaatgcggcg tttctattaa tacagacgtt aagctcagaa cagcgacttc taaaagcctg
    18721 gttaaccggg cattaaggca caataatcat actttatgaa gaatatacgt aacttttcga
    18781 tcatagctca cattgaccac ggtaaatcga cgctgtctga ccgtattatc cagatctgcg
    18841 gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga ttccatggat cttgagcgtg
    18901 agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga ctacaaagcg tctgacggcg
    18961 aaacctatca gcttaacttt atcgacaccc cgggccacgt agacttctcc tatgaagttt
    19021 cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt cgacgccggg cagggcgtag
    19081 aagcgcaaac cctggcaaac tgctacaccg ccatggaaat ggatctcgaa gtggtgccgg
    19141 tactgaacaa gattgacctg ccggcagccg atcctgaacg cgtggcggaa gaaattgaag
    19201 atatcgtcgg catcgacgcc accgatgcag tgcgctgttc agcgaaaacc ggcgttggcg
    19261 tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc gccggaaggc gatccggaag
    19321 gcccgttgca ggcactaatt atcgactcct ggttcgacaa ctacctgggc gttgtttcac
    19381 ttatccgtat taaaaacggc accctgcgta agggcgacaa agtgaaagtc atgagtaccg
    19441 ggcagaccta taacgccgac cgtctgggca tcttcacgcc gaaacaggtt gaccgcactg
    19501 aactgaaatg tggtgaagta ggctggctcg tatgtgcgat taaagatatc cacggcgctc
    19561 cagtcggcga taccttaacg ctggcgcgta atccggcaga aaaggcgctg cctggcttta
    19621 agaaagtcaa accgcaggta tacgccggtc tgttcccggt aagttccgac gactatgaag
    19681 ccttccgtga cgcgctgggt aaactcagcc tgaacgatgc ctcactgttc tatgagccgg
    19741 aaagctccag cgcgctgggc tttggtttcc gctgcggctt ccttggcctg ctgcacatgg
    19801 agatcatcca ggagcgtctg gaacgtgaat acgatctgga tctgatcacc actgcgccga
    19861 ccgtagtgta tgaagttgaa accacgtcaa gggaagttat ctacgtcgac agcccatcca
    19921 agctgcctgc ggtaaataac atttacgaac tgcgcgagcc gattgcagag tgtcacatgc
    19981 tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg cgtagaaaaa cgcggcgtgc
    20041 agaccaatat ggtttaccac ggtaatcagg tggcgctgac gtacgagatc ccgatggcgg
    20101 aagtggtgct cgacttcttc gatcgcctga aatctacctc gcgtggttat gcgtctctgg
    20161 attacaactt caaacgcttc caggcgtccg acatggtacg tgtagacgta ttaatcaacg
    20221 gtgaacgtgt tgatgcgctg gcgttgatca cccaccgtga taattcgcaa aaccgaggtc
    20281 gcgagttggt ggagaagatg aaagatctga tcccacgcca gcagtttgat atcgccattc
    20341 aggcagcgat tggtacgcac attattgcgc gatccaccgt gaaacagctg cgtaaaaacg
    20401 tactggctaa atgttatggc ggcgatatca gccgtaagaa aaagctgctg cagaagcaga
    20461 aagaaggtaa gaaacgcatg aagcagatcg gtaacgttga gctgccgcag gaagcgttcc
    20521 tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc atggcgaata
    20581 tgtttgccct gattctggtg attgccacac tggtgacggg cattttatgg tgcgtggata
    20641 aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg gctgccggtg
    20701 actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa gcctggctgg ctggaaaccg
    20761 gagcttctgt ttttccggtg ctggctatcg tattgattgt acgttcgttt atttatgaac
    20821 cgttccagat cccgtcaggt tcgatgatgc cgactctgtt aatcggtgat tttattctgg
    20881 tagagaagtt tgcttatggc attaaagatc ctatctacca gaaaacgctg atcgaaaccg
    20941 gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca aagcttgatt
    21001 acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttacgatccg gtctcaaaag
    21061 agctgacgat tcaaccggga tgcagttccg gccaggcgtg tgaaaacgcg ctgccggtca
    21121 cctactcaaa cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt aatggtgggg
    21181 aagcgaccag cggattcttt gaagtgccga aaaacgaaac caaagaaaat ggaattcgtc
    21241 tttccgagcg taaagagaca ctgggtgatg tgacgcaccg aattctgaca gtgccgattg
    21301 cgcaggacca ggtggggatg tattaccagc agccagggca acaactggca acctggattg
    21361 ttccgccggg acaatacttc atgatgggcg acaaccgcga caacagcgcg gacagccgtt
    21421 actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc cacggctatc tggatgagct
    21481 tcgataagca agaaggcgaa tggccgactg gtgtgcgctt aagtcgcatt ggcggcatcc
    21541 attaatagcc atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga
    21601 gataaatctc ccgtggctaa cgacatcccc cgtcgttgtg tatagaatat tcccccgaag
    21661 tttaaggttg gcacctccag gttgccacgg cacacgaaac agcgttggtc cccatatacc
    21721 gctaaactga aactgcagcg aagcagttag cagagccatg tatatcaggt ctgtttcgtg
    21781 tgctgaattg ttgacgcatt tatttattgg tatcgcatga accccatcgt aattaatcgg
    21841 cttcaacgga agctgggcta cacttttaat catcaggaac tgttgcagca ggcattaact
    21901 catcgtagtg ccagcagcaa acataacgag cgtttagaat ttttaggcga ctctattctg
    21961 agctacgtta tcgccaatgc gctttatcac cgtttccctc gtgtggatga aggcgatatg
    22021 agccggatgc gcgccacgct ggtccgtggc aatacgctgg cggaactggc gcgcgaattt
    22081 gagttaggcg agtgcttacg tttagggcca ggtgaactta aaagcggtgg atttcgtcgt
    22141 gagtcaattc tcgccgacac cgtcgaagca ttaattggtg gcgtattcct cgacagtgat
    22201 attcaaaccg tcgagaaatt aatccttaac tggtatcaaa ctcgtctgga cgaaattagc
    22261 ccaggcgata aacaaaaaga tccgaaaacg cgcttgcaag aatatttgca gggtcgccat
    22321 ctgccgctgc cgacttatct ggtagtccag gtacgtggcg aagcgcacga tcaggaattt
    22381 actatccact gccaggtcag cggcctgagt gaaccggtgg ttggcacagg ttcaagccgt
    22441 cgtaaggctg agcaggctgc cgccgaacag gcgttgaaaa aactggagct ggaatgagca
    22501 tcgataaaag ttactgcgga tttattgcca tcgtcggacg tccgaacgtt ggcaaatcca
    22561 cattgttgaa caaactgctg gggcagaaaa tctccatcac ctcccgcaag gcgcagacaa
    22621 ctcgtcaccg cattgtgggg atccatactg aaggcgcgta tcaggcgatc tacgtcgata
    22681 caccgggcct gcatatggaa gaaaaacgcg ccattaaccg cctgatgaac aaagcggcga
    22741 gcagctctat tggcgatgtt gagctggtga tttttgtcgt tgaaggcacc cgctggacgc
    22801 cggacgacga aatggtgctc aacaaactgc gcgaaggcaa agcgccggta atcctcgcgg
    22861 tgaacaaagt ggacaacgtg caggagaaag ccgatctgct gccgcacctg cagttcctgg
    22921 caagccagat gaacttcctc gatatcgtgc caatctctgc cgaaaccggg ctgaatgttg
    22981 acactattgc ggcaatcgtg cgtaagcatc tgcctgaagc gactcatcac ttcccggaag
    23041 attacatcac cgatcgctca cagcgtttta tggcgtctga aatcatccgc gaaaaactga
    23101 tgcgtttcct tggcgctgaa ctgccgtact ccgtgaccgt ggagatcgaa cgtttcgtct
    23161 ctaacgaacg cggcggttgt gacatcaacg gtttgattct cgttgagcgt gaagggcaga
    23221 agaagatggt tattggcaac aaaggggcca agatcaaaac catcgggatt gaagcgcgta
    23281 aagacatgca ggaaatgttc gaagcgcctg tccaccttga actgtgggta aaagtgaaat
    23341 cgggttgggc cgacgacgaa cgcgcactgc gcagtctcgg ttatgttgac gatctttaag
    23401 agtcactccg atggaaggct ggcagcgcgc atttgtcctg catagtcgcc cgtggagcga
    23461 aaccagcctg atgctggacg tcttcacaga ggaatcgggt cgcgtgcgtc tggttgccaa
    23521 aggcgcacgc tctaaacgct ctactctgaa aggtgcatta cagcctttca cccctctctt
    23581 gctacgtttt ggcgggcgtg gcgaagtcaa aacgctgcgc agtgctgaag ctgtctcact
    23641 ggcgctgcca ttaagcggta tcacgcttta cagcggtctg tacatcaacg aacttctctc
    23701 ccgcgtactg gaatacgaga cgcgcttctc tgaactcttt ttcgattact tgcactgcat
    23761 tcagtctctt gcaggtgaca ctggtacgcc agaacccgcg ctgcgtcgct ttgaactggc
    23821 actactcggg catctgggct atggcgtcaa ttttacccat tgcgcgggta gcggcgagcc
    23881 ggtagatggc accatgacgt atcgttatcg cgaagaaaaa gggtttatcg caagcgtcgt
    23941 tatcgacaat aaaacgttca ccggaaggca gttaaaagcg ttaaacgcgc gggaatttcc
    24001 tgacgcagac acactacgcg ccgcgaaacg ctttacccga atggcgctta agccgtatct
    24061 tggcggtaaa ccattaaaga gcagggaact gttccggcag tttatgccta agcgaacggt
    24121 gaaaacacat tatgaatgat gaggattgtc atggctgaat tactgttagg cgtcaacatt
    24181 gaccatattg caacgctgcg taacgcacgc ggtactgctt acccggatcc ggtgcaggcg
    24241 gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg tgaagatcgc
    24301 cgccacatta ctgaccgcga tgtgcgcatc ctgcggcaga cgctggatac ccgaatgaat
    24361 ctggagatgg cagtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt
    24421 tgctgcttgg taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggacgttgca
    24481 gggcagcgtg aaaaaatacg cgatgcctgc aaacgtctgg cagatgccgg gattcaggtt
    24541 tctctgttta tcgacgccga tgaagagcag atcaaagctg cggcagaggt tggcgcaccg
    24601 tttatcgaga tccacaccgg ttgctatgct gatgccaaaa ctgacgccga acaggcgcaa
    24661 gagctggtgc gtatcgccaa agccgcgacc tttgccgcta gtctcggtct gaaagttaac
    24721 gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat
    24781 gaactgaata tcggtcatgc cattattggc cgtgcagtga tgaccggact gaaagatgcg
    24841 gtggcagaaa tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag
    24901 gcacggatat cgtagagatc gctcgcatag aagcagtgat cgcccgatcc ggtgagcgcc
    24961 tggcacgccg cgtattaagc gataacgaat gggcaatctg gaaaacgcac caccagccgg
    25021 tacgttttct ggcgaagcgt tttgctgtga aagaagccgc agcaaaagcg tttggcaccg
    25081 ggatccgcaa tggtctggcg tttaatcaat ttgaagtatt caatgatgag ctcggcaaac
    25141 cacggctacg gctatggggt gaggcattaa aactggcgga aaagctcggc gttgtaaata
    25201 tgcatgtaac gctggcagat gaacgccact atgcttgtgc cacggtaatt attgaaagtt
    25261 aaaataatac cgttctcttt aaaaagtaaa acccccgaac tggtaaggaa cgggggtttt
    25321 actttttaac tttaattttg tgcagcatgc accctcaaca acagcgatta tggatcagtg
    25381 tacttatctt ttacaagcaa cttgcaatct ttagcataaa aactcgagcc tttacgaaga
    25441 aagcaatatt gatggaaaga ttaacgtgac cgccaattcg taagtacatt aaaattggct
    25501 tcgttattga agattttgct gtgctttaca ccatgccaca gaattccccc attgaaacga
    25561 gtggtgtcgt caaagctctg gtgtggagtg cagcatgcac cctcaataac tcgcacgttc
    25621 agttttgggg agatgtaagg gctaatctga atggctgcat tccttgttta aggaaaaacg
    25681 aatgactgat tgccgatacc tgattaaacg ggtcatcaaa atcatcattg ctgttttaca
    25741 gctgatcctt ctgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa
    25801 tcggacgcgc ctttaataac tataaataag tgtcttggca gatactatat aaattaactt
    25861 agtgaatgat tatgctaatg tcatcaatta aataaatata atggcgttaa ggcttcccag
    25921 taatataatt aatactctac ttccagagta gaatattaaa ttttatccgc gtggtgcatc
    25981 agcacaaatt tatcccacaa ctgttcttct gtctcgacat gcgccggatc tttcacaata
    26041 gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc tacgcattcg
    26101 gtacacttat cgctgttaat ttcgtagata tgatctccca ttgaaatcgc ctcattcggg
    26161 cattcgggtt cacacatatc acaattaata cagcgtttag taattagtaa agacatttca
    26221 atggattacc gttaaatcat tttaaaatca gtaagttgta tcgagtttgt atgctttact
    26281 gtcattaact tactgtatgt tgatccagtg tatttaacct tgataaactc agtccagcaa
    26341 cacaaaaccg caacacattg cattttgtcc cgtagaaaag acttgtatgt gtgagcttgt
    26401 tttctgcgcc tacgcagata aggattgaga atgccgcgca ctgtaacaca taatccggat
    26461 agccccaata atgacgatgt tttagccgca tctgaaaaat gggacgcctg taaacccccc
    26521 tataccagcg cacacatgaa aatctgtgtt gctgccgcca aaatcatcct cgctgcttcc
    26581 ggcgtggctc gccgttccaa atacgaaaaa gagaactatc tccgtatcga tttcagcaaa
    26641 gccggtaagg ttacatttta cgccgagttt ccaaaaaaga tgggcctcaa aggtaaaaag
    26701 ctcggcgagt ggccggagct cgttatccag ctggcgcgcg aaaaagcact aggtatggct
    26761 gacggtggac tgcgggcaga gtccgtacat gcagcgctgg aaatgtaccg ggatgaccca
    26821 aagccaaagt agcccggcag aagctgagcc cggacagttt cacaacctac ggggtgcgta
    26881 tcgaccggat taaagcaacg ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc
    26941 ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac gccctggagt
    27001 tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc aacggccgca
    27061 atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa cctaccccca
    27121 cacggctttt taccgatatt gaatcaatcg cccgactctg gctcaatgtt gctgcctgca
    27181 cctctgtaca ccagaagaat gctgttcgct tcatgatcat cactggtgtt cgtccgatta
    27241 atgtccataa cctgcgctgg gactacgttt acgaggaggc tggtgaaatt gtttatccgg
    27301 aaggggttat cggcatgcga ggggctatga aaacacaaaa ggctttccgc ctgccgataa
    27361 cgcctgagat ccggcggatt atcgacgagc agaaagcctg gcgtgattca gttcctgagt
    27421 gcaacaggga ttatgtattt ttgcagccac gtgatccaat gcagccattt tcaaaacgat
    27481 cactggataa gctggtgaaa acatacagcc cggacggggc tgtaaaatga ggtgtactgg
    27541 caatagcgga cactaccatt tgttcttttt ttaagcagcc atctgatgat
//
LOCUS       DAEBXD010000050        27158 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEBXD010000050 DAEBXD010000000
VERSION     DAEBXD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27158
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.050"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            56..850
                     /locus_tag="KJL94_002243"
     CDS             56..850
                     /locus_tag="KJL94_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD4410580.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="KJL94_002244"
     CDS             872..2281
                     /locus_tag="KJL94_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410581.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="KJL94_002245"
     CDS             2259..3038
                     /locus_tag="KJL94_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBD4410582.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="KJL94_002246"
     CDS             3035..3895
                     /locus_tag="KJL94_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBD4410583.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="KJL94_002247"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="KJL94_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="KJL94_002248"
     CDS             complement(4635..4895)
                     /locus_tag="KJL94_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD4410584.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="KJL94_002249"
     CDS             complement(4886..6157)
                     /locus_tag="KJL94_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD4410585.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="KJL94_002250"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="KJL94_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBD4410586.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="KJL94_002251"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="KJL94_002251"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBD4410587.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="KJL94_002252"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="KJL94_002252"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBD4410588.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="KJL94_002253"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="KJL94_002253"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBD4410589.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="KJL94_002254"
     CDS             11499..11651
                     /locus_tag="KJL94_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD4410590.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="KJL94_002255"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="KJL94_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="KJL94_002256"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="KJL94_002256"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBD4410591.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="KJL94_002257"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="KJL94_002257"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBD4410592.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="KJL94_002258"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="KJL94_002258"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBD4410593.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="KJL94_002259"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="KJL94_002259"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBD4410594.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="KJL94_002260"
     CDS             17363..17686
                     /locus_tag="KJL94_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBD4410595.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="KJL94_002261"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="KJL94_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBD4410596.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="KJL94_002262"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="KJL94_002262"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBD4410597.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="KJL94_002263"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="KJL94_002263"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBD4410598.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="KJL94_002264"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="KJL94_002264"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBD4410599.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="KJL94_002265"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="KJL94_002265"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBD4410600.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="KJL94_002266"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="KJL94_002266"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBD4410601.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="KJL94_002267"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="KJL94_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBD4410602.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="KJL94_002268"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="KJL94_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBD4410603.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="KJL94_002269"
     CDS             complement(24261..24668)
                     /locus_tag="KJL94_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBD4410604.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="KJL94_002270"
     CDS             24815..25408
                     /locus_tag="KJL94_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBD4410605.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="KJL94_002271"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="KJL94_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..>27158)
                     /locus_tag="KJL94_002272"
     CDS             complement(26628..>27158)
                     /locus_tag="KJL94_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410606.1"
                     /translation="DGKEPIIERIFRLVERYPSRNAAARAWGINEGTLKNYYNRRDYA
                     PIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDAQILMFLSFLKP
                     QEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLPDEKVREIYALS
                     EAKGDHLNLPQKAASA"
BASE COUNT         6560 a         6771 c         7340 g         6487 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggcgtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacca gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ttggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcaattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggatcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt caggggcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccggataa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggct cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga tgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcacgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat taccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat gtgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatc
//
LOCUS       DAEBXD010000051        26076 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEBXD010000051 DAEBXD010000000
VERSION     DAEBXD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.051"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KJL94_002273"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KJL94_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBD4410607.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KJL94_002274"
     CDS             complement(2138..2584)
                     /locus_tag="KJL94_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBD4410608.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KJL94_002275"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KJL94_002275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBD4410609.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KJL94_002276"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KJL94_002276"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBD4410610.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KJL94_002277"
     CDS             complement(5347..6009)
                     /locus_tag="KJL94_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBD4410611.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KJL94_002278"
     CDS             6182..6595
                     /locus_tag="KJL94_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBD4410612.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KJL94_002279"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KJL94_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBD4410613.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KJL94_002280"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KJL94_002280"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBD4410614.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KJL94_002281"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KJL94_002281"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBD4410615.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KJL94_002282"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KJL94_002282"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBD4410616.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KJL94_002283"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KJL94_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBD4410617.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KJL94_002284"
     tRNA            complement(9861..9948)
                     /locus_tag="KJL94_002284"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KJL94_002285"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KJL94_002285"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBD4410618.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KJL94_002286"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KJL94_002286"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBD4410619.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KJL94_002287"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KJL94_002287"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBD4410620.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KJL94_002288"
     CDS             14005..14592
                     /locus_tag="KJL94_002288"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBD4410621.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KJL94_002289"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KJL94_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBD4410622.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KJL94_002290"
     CDS             14984..15841
                     /locus_tag="KJL94_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBD4410623.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KJL94_002291"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KJL94_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBD4410624.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVVTGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KJL94_002292"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KJL94_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD4410625.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KJL94_002293"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KJL94_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBD4410626.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KJL94_002294"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KJL94_002294"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBD4410627.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KJL94_002295"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KJL94_002295"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBD4410628.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KJL94_002296"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KJL94_002296"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBD4410629.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="KJL94_002297"
     CDS             complement(22898..24037)
                     /locus_tag="KJL94_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBD4410630.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="KJL94_002298"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="KJL94_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="KJL94_002299"
     CDS             complement(25322..26017)
                     /locus_tag="KJL94_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4410631.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6647 c         6906 g         6306 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggttac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DAEBXD010000052        26057 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEBXD010000052 DAEBXD010000000
VERSION     DAEBXD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26057
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.052"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            348..641
                     /locus_tag="KJL94_002300"
     CDS             348..641
                     /locus_tag="KJL94_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBD4410632.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="KJL94_002301"
     CDS             713..1372
                     /locus_tag="KJL94_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBD4410633.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="KJL94_002302"
     CDS             1464..1910
                     /locus_tag="KJL94_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBD4410634.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="KJL94_002303"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="KJL94_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="KJL94_002304"
     CDS             3390..3809
                     /locus_tag="KJL94_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD4410635.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="KJL94_002305"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="KJL94_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBD4410636.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="KJL94_002306"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="KJL94_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBD4410637.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="KJL94_002307"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="KJL94_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBD4410638.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="KJL94_002308"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="KJL94_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBD4410639.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="KJL94_002309"
     CDS             complement(8332..9864)
                     /locus_tag="KJL94_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBD4410640.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="KJL94_002310"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="KJL94_002310"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBD4410641.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="KJL94_002311"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="KJL94_002311"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBD4410642.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="KJL94_002312"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="KJL94_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="KJL94_002313"
     CDS             complement(14765..16501)
                     /locus_tag="KJL94_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBD4410643.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="KJL94_002314"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="KJL94_002314"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBD4410644.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="KJL94_002315"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="KJL94_002315"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBD4410645.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="KJL94_002316"
     CDS             complement(18223..18957)
                     /locus_tag="KJL94_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBD4410646.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="KJL94_002317"
     CDS             19158..19412
                     /locus_tag="KJL94_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBD4410647.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="KJL94_002318"
     CDS             complement(19462..20922)
                     /locus_tag="KJL94_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBD4410648.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="KJL94_002319"
     CDS             complement(20859..21416)
                     /locus_tag="KJL94_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBD4410649.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITPLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="KJL94_002320"
     CDS             complement(21442..22296)
                     /locus_tag="KJL94_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBD4410650.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="KJL94_002321"
     CDS             complement(22296..23105)
                     /locus_tag="KJL94_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBD4410651.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="KJL94_002322"
     CDS             complement(23115..23873)
                     /locus_tag="KJL94_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4410652.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="KJL94_002323"
     CDS             complement(23870..24850)
                     /locus_tag="KJL94_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBD4410653.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="KJL94_002324"
     CDS             complement(24850..25872)
                     /locus_tag="KJL94_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBD4410654.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6522 a         6395 c         6744 g         6396 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agtacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcaggggggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggcta
//
LOCUS       DAEBXD010000053        25212 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEBXD010000053 DAEBXD010000000
VERSION     DAEBXD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25212
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.053"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            2..976
                     /locus_tag="KJL94_002325"
     CDS             2..976
                     /locus_tag="KJL94_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD4410655.1"
                     /translation="MFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSPD
                     DEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASNA
                     LGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYKQ
                     AALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGEG
                     VDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDAT
                     AWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(1046..1528)
                     /locus_tag="KJL94_002326"
     CDS             complement(1046..1528)
                     /locus_tag="KJL94_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBD4410656.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            1763..4345
                     /gene="leuS"
                     /locus_tag="KJL94_002327"
     CDS             1763..4345
                     /gene="leuS"
                     /locus_tag="KJL94_002327"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBD4410657.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4360..4941
                     /gene="lptE"
                     /locus_tag="KJL94_002328"
     CDS             4360..4941
                     /gene="lptE"
                     /locus_tag="KJL94_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBD4410658.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            4941..5972
                     /gene="holA"
                     /locus_tag="KJL94_002329"
     CDS             4941..5972
                     /gene="holA"
                     /locus_tag="KJL94_002329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBD4410659.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5974..6615
                     /gene="nadD"
                     /locus_tag="KJL94_002330"
     CDS             5974..6615
                     /gene="nadD"
                     /locus_tag="KJL94_002330"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBD4410660.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6639..7250
                     /locus_tag="KJL94_002331"
     CDS             6639..7250
                     /locus_tag="KJL94_002331"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBD4410661.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7510..7827
                     /gene="rsfS"
                     /locus_tag="KJL94_002332"
                     /gene_synonym="rsfA"
     CDS             7510..7827
                     /gene="rsfS"
                     /locus_tag="KJL94_002332"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBD4410662.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7831..8298
                     /gene="rlmH"
                     /locus_tag="KJL94_002333"
     CDS             7831..8298
                     /gene="rlmH"
                     /locus_tag="KJL94_002333"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBD4410663.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8329..10230
                     /gene="mrdA"
                     /locus_tag="KJL94_002334"
     CDS             8329..10230
                     /gene="mrdA"
                     /locus_tag="KJL94_002334"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBD4410664.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10233..11345
                     /gene="mrdB"
                     /locus_tag="KJL94_002335"
                     /gene_synonym="rodA"
     CDS             10233..11345
                     /gene="mrdB"
                     /locus_tag="KJL94_002335"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBD4410665.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGMWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11356..12444
                     /gene="rlpA"
                     /locus_tag="KJL94_002336"
     CDS             11356..12444
                     /gene="rlpA"
                     /locus_tag="KJL94_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBD4410666.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12583..13794
                     /gene="dacA"
                     /locus_tag="KJL94_002337"
     CDS             12583..13794
                     /gene="dacA"
                     /locus_tag="KJL94_002337"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBD4410667.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13906..14169
                     /locus_tag="KJL94_002338"
     CDS             13906..14169
                     /locus_tag="KJL94_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBD4410668.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14270..14911
                     /gene="lipB"
                     /locus_tag="KJL94_002339"
     CDS             14270..14911
                     /gene="lipB"
                     /locus_tag="KJL94_002339"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBD4410669.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15170..16123
                     /locus_tag="KJL94_002340"
     CDS             15170..16123
                     /locus_tag="KJL94_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD4410670.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            16332..17297
                     /gene="lipA"
                     /locus_tag="KJL94_002341"
     CDS             16332..17297
                     /gene="lipA"
                     /locus_tag="KJL94_002341"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBD4410671.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17398..17601)
                     /gene="tatE"
                     /locus_tag="KJL94_002342"
     CDS             complement(17398..17601)
                     /gene="tatE"
                     /locus_tag="KJL94_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBD4410672.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17730..18518)
                     /locus_tag="KJL94_002343"
     CDS             complement(17730..18518)
                     /locus_tag="KJL94_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBD4410673.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            18611..18994
                     /gene="crcB"
                     /locus_tag="KJL94_002344"
     CDS             18611..18994
                     /gene="crcB"
                     /locus_tag="KJL94_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBD4410674.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(19048..19257)
                     /gene="cspE"
                     /locus_tag="KJL94_002345"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19048..19257)
                     /gene="cspE"
                     /locus_tag="KJL94_002345"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD4410675.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19432..19992)
                     /gene="pagP"
                     /locus_tag="KJL94_002346"
     CDS             complement(19432..19992)
                     /gene="pagP"
                     /locus_tag="KJL94_002346"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBD4410676.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20580..21965
                     /gene="dcuC"
                     /locus_tag="KJL94_002347"
     CDS             20580..21965
                     /gene="dcuC"
                     /locus_tag="KJL94_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD4410677.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(22006..22347)
                     /locus_tag="KJL94_002348"
                     /pseudo
     CDS             complement(22006..22347)
                     /locus_tag="KJL94_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(22685..23194)
                     /locus_tag="KJL94_002349"
     CDS             complement(22685..23194)
                     /locus_tag="KJL94_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD4410678.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            23357..23602
                     /locus_tag="KJL94_002350"
     CDS             23357..23602
                     /locus_tag="KJL94_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4410679.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            23577..25208
                     /locus_tag="KJL94_002351"
                     /pseudo
     CDS             23577..25208
                     /locus_tag="KJL94_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT         6378 a         6204 c         6296 g         6334 t
ORIGIN      
        1 gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga
       61 aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat
      121 tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgctg agcaaggtca
      181 ttgtgaagca cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga
      241 taatgagcaa gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc
      301 caacgccctt ggctggatac tggatcgtgg agaagacccc aactataaag aagcggttac
      361 ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc ttggatggat
      421 gtacagaaat ggcaacggag tcgcacaaga ctatacgctg gcatttttct ggtacaaaca
      481 agctgcatta caaggccata gttacgcaca agacaatctg gccgatcttt ataaagacgg
      541 agaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa gcgcacagca
      601 gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg aaggggtgga
      661 ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg aaagcgtcgg
      721 cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca
      781 ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa
      841 cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga
      901 cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac
      961 tgaagcttta ctgtagaaat aaaaacaagg gccggggaat ttcattctgc ccatattctc
     1021 tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat
     1081 aaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac
     1141 atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat
     1201 gattgaggtc ctggaaaatt tcgcgccatt caagctgcgt tttatcggtg atgtctgcca
     1261 gctcctgcca caagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca
     1321 ggctctcttc atagctcatg gcgaactctt ccaggtcacg tctgacagct cgcgtcagct
     1381 catcgatctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca
     1441 ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gttcagtgac gcgaccagtt
     1501 cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata
     1561 ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg
     1621 tacgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac
     1681 gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg tagccgtatt
     1741 gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga tagaatccaa
     1801 agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg agagcaaaga
     1861 gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca tgggccacgt
     1921 acgtaactac accatcggtg acgtgatcgc ccgttaccag cgcatgctgg gcaaaaacgt
     1981 tctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg cggtgaaaaa
     2041 caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa accagctcaa
     2101 aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc cggaatacta
     2161 ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat ataaaaagac
     2221 ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac aagttatcga
     2281 cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc agtggtttat
     2341 caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg atcactggcc
     2401 agacaccgtt aaaaccatgc agcgtaactg gatcggtcgt tccgaaggcg tggagatcac
     2461 cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc cggacacctt
     2521 tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga aagcggcgga
     2581 aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag ttgccgaagc
     2641 tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg ttcacccatt
     2701 aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt acggcacggg
     2761 cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct ctaaatacgg
     2821 cctgaacatc aaaccggtta tcctggcggc tgacggctct gagccagatc tttctcagca
     2881 agccctgact gaaaaaggcg tgctgttcaa ctctggcgag ttcaacggtc ttgaccatga
     2941 agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg agcgtaaagt
     3001 gaactaccgc ctgcgcgact ggggtgtttc tcgccagcgt tactggggcg cgccgattcc
     3061 gatggtgacg ctggaagacg gtaccgtaat gccaaccccg gacgaccagc tgccggtgat
     3121 cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag atccggagtg
     3181 ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt tcgacacctt
     3241 tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag aaggtatgct
     3301 ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg gtatcgaaca
     3361 cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg atgcaggcat
     3421 ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc
     3481 cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg atgctatcgt
     3541 tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt
     3601 ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat
     3661 ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt ctccggctga
     3721 tatgactctc gaatggcagg aatccggtgt ggaaggggct aaccgcttcc tgaaacgtgt
     3781 ctggaaactg gtgtacgagc acacagcaaa aggtgatgtt gcggcactga acgttgatgc
     3841 gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaactatcg ctaaagtgac
     3901 cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta tggagctgat
     3961 gaacaaactg gcgaaagcac cgaccgatgg cgagcaggat cgcgctctga tgcaggaagc
     4021 actgcaggcc gttgtccgta tgctcaaccc gttcaccccg cacatctgct tcacgctgtg
     4081 gcaggaactc aaaggcgaag gcgatatcga caacgcgccg tggccggttg ctgacgaaaa
     4141 agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag tccgtgccaa
     4201 aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg gccaggaaca
     4261 tctggtagca aaatatcttg atggcgttac tgtacgtaaa gtgatttacg taccaggtaa
     4321 actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct ggcaacactg
     4381 ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg tgacaccacg
     4441 caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa cgggccatta
     4501 agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga taaagaaacc
     4561 acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca
     4621 tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt gaatgcgacc
     4681 gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg ttcattcttc
     4741 gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt aaaagagatg
     4801 tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc tgcggatatt
     4861 cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc
     4921 tccaccatgc tgggtaactg atgattcggt tgtacccgga acaactccgc gcgcagctca
     4981 atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta ttgcaggaaa
     5041 gccaggacgc tgttcgccag gtagctgcgg cacaaggatt cgaagaacac cacacttttt
     5101 ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct atgagtctgt
     5161 ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat gcggcgatca
     5221 atgaacaact tctcacactc accggacttc tgcatgacga cctgctattg atcgtccgcg
     5281 gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgctctt gcgaatcgca
     5341 gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg gttgctgtgc
     5401 gcgcaaaaca gctcaactta gaactggatg acgcggcgaa tcaggtgctc tgctactgtt
     5461 atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg ctctggccag
     5521 acggcaaact gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc
     5581 cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc
     5641 agcaactgcg tctggaaggc agcgagccgg ttattttgtt gcgcacatta caacgtgaac
     5701 tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg
     5761 ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa
     5821 gtcagccgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc accctcaaac
     5881 aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg ttgtgccata
     5941 aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc tctgtttggc
     6001 ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg
     6061 attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg tccccagccg
     6121 gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga caagccatta
     6181 tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc gcaaacactg
     6241 aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat tggtcaggat
     6301 tcattgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa tgcacatttg
     6361 atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata ccagcaatgg
     6421 ctggaagatc atttgacaca taacccggaa gatcttcacc ttcagcctgc cggtaaaatt
     6481 tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga acgtttgcaa
     6541 aacggtgaat cgtgtgagga tttattgccg gaaccggtat tgacttacat taaccaacaa
     6601 ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc
     6661 atggcgaaac acaagcgaat gtcgatggtc tgtacagcgg tcatgcgccc acacccctga
     6721 ccgcacgcgg tattgagcaa gcgcaaaatc tgcatacact gctagatgat gtttcctttg
     6781 acctggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg gttctcagtg
     6841 accgccagct ccccgttcat atcatccctg aactcaacga aatgtttttt ggcgactggg
     6901 agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc gcgtggtgca
     6961 atgactggca gcatgctatc cccacgaatg gtgaaggatt tcaggcattt tcgcaacgtg
     7021 tggaacgctt tatcgcaagg cttagtgaat atcagcacta tcagaatatt ttaatcgtca
     7081 gccatcaggg cgtactgagt ctgttaattg ctcgtttaat tggcatgcct gccgagtcta
     7141 tgtggcattt tcgcgttgac caggggtgtt ggagcgccat tgatatcaac caaaaattcg
     7201 cgacgctacg cgtcctcaat agccgtgcca tcggggtcga gaacgcatga cttttctgtt
     7261 tttttacggg taagccgcaa cgaccattga cagccccggg caggctgata ttctccgcag
     7321 ccagactttt tccgccagac acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa
     7381 tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg acaattgtcc
     7441 cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg attcacccag
     7501 ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat tgatgacctc
     7561 aaaggtcagg acatcatcgc cttagacgtt caaggcaaat ccagcatcac cgactgcatg
     7621 atcatctgta cgggtacgtc cagccgtcat gttatgtcca ttgctgacca cgttgtgcag
     7681 gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg
     7741 attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg
     7801 tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg
     7861 aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt tccgaaagat
     7921 atgcccttcg agctgattga aattccggcc ggcaaacgcg gcaagaatgc ggacatcaag
     7981 cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc
     8041 accctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga gctggaacgc
     8101 tggaagctgg atggtcgcga cgtaagtcta ctgattggcg ggcctgaagg gttgtcgcct
     8161 gcctgtaaag cggcggctga gcagagctgg tcgctgtcgg cgcttaccct cccccatccg
     8221 ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac caccaaccat
     8281 ccttatcacc gtgagtgata agggagcttt gagtagaaaa cgcagcggat gaaactacag
     8341 aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc
     8401 tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa tctgcaaatt
     8461 gttcgcttta ccgactacca gacccgctct aatgaaaacc gcattaagct ggtgcctatc
     8521 gcgcccagcc gcggcattat ctacgaccgt aacggtatcc ctctggccct caaccgcact
     8581 atctaccaga tagaaatgat gccggagaaa gtcgataacg tgcagcaaac gctggacgct
     8641 ttgcgcagcg tggtagatct gaccgatgac gatattgctg cattccgaaa agagcgcgca
     8701 cgttcacacc gtttcacctc tattccggtg aaaactaacc taaccgaagt acaagtagct
     8761 cgctttgccg tcaatcagta ccgttttccg ggtgtcgaag ttaaaggcta taaacgtcgt
     8821 tactatcctt acggttcggc gttgacccac gtcatcggct atgtgtcgaa aatcaacgat
     8881 aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc aacgcatgat
     8941 attggtaagc tgggcattga gcgttactat gaagacgtgc tgcacggtca gaccggttat
     9001 gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg
     9061 caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata tattgaaacg
     9121 ctgctggcgg gtagccgcgc agctgtggtt gttaccgaac cgcgtacagg tggggtgctg
     9181 gcgctggttt ccacgcctag ttatgaccca aacctgtttg ttgacggtat ctccagcaaa
     9241 gattattccg ccttgctgaa cgacccgaat acaccgctgg tgaaccgcgc cacacagggg
     9301 gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt aagcgccggg
     9361 gtgatcacgc gcaatacgac gctgtttgac ccaggctggt ggcaactacc agggtcggaa
     9421 aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg
     9481 gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat cgatcgcctc
     9541 tccgaatgga tgggtaaatt cggttatggt cattacaccg gtatcgacct ggcggaagaa
     9601 cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat
     9661 cagggtgaca ccattccggt tggtatcggc cagggttact ggacagcgac cccaatccag
     9721 atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc gcatttgctg
     9781 atgagcaccg ccgaagacgg caaacaggtg ccatgggtac aaccgcatga accgcccgtc
     9841 ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac
     9901 cgccctaatg gtacggcgaa taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa
     9961 tctggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt
    10021 gccgagcgtt tacgtgacca caaactgatg accgcctttg cgccatacaa caatccgcaa
    10081 gtggctgtcg ccatgattct ggagaacggc ggcgcgggtc cggcggttgg tacgctgatg
    10141 cgccagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc tgcggaaaat
    10201 ccagcggttg ccgcagcgga ggaccattaa tcatgacgga taatccgaat aaaaaaacat
    10261 tctgggataa agtccatctc gatcccacaa tgctgctgat tttactggca ttgctggttt
    10321 acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa
    10381 tcggccaaat cgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg
    10441 tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg gtggcggtag
    10501 atgctttcgg agccatctct aaaggcgctc aacgctggct ggacctcggt attgttcgtt
    10561 ttcagccgtc ggaaattgcc aaaatagccg taccactgat ggttgcgcgc tttatcaacc
    10621 gcgacgtttg cccgccatcg ttgaagaata ctggcatcgc gctggtgctg atatttatgc
    10681 ccacgctgct ggtggctgca cagcctgacc tgggaacatc aatcctcgtt gcgctttccg
    10741 gtctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc gcagtagtgc
    10801 tggtagcggc gttcattccg attctgtggt tcttcctgat gcatgattac cagcgccagc
    10861 gcgtaatgat gctcctggac ccggaatcag acccactcgg cgcgggctat cacattattc
    10921 agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg cacggcactc
    10981 agtcacagct tgaatttctc cccgaacgcc atactgactt tatcttcgcg gtactggcgg
    11041 aagagctggg attagtgggc attctgattc tgctcgctct ctacattctg ctgatcatgc
    11101 gcgggatgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg gctggtggct
    11161 taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc ggtattctgc
    11221 cggttgtagg ggttccgctc ccactggtca gttatggagg atcggcgcta attgtgctga
    11281 tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg
    11341 tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc aggaatgctc
    11401 gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta
    11461 tgtaacggcc ccatagttga aattagcggg gcggacccgc gtttcgaacc actgaacgcg
    11521 acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct
    11581 cgatttagcc aggcgggact ggcggcaatc tatgatgccg aaccaggcag taacctgacg
    11641 gcctctggcg aagctttcga tccgacaaag ctgacggcgg cccatccaac gcttccgatc
    11701 cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt gcgcattaat
    11761 gatcgcggcc cttacggcaa cgaccgcgtt atttcgcttt ctcgcgcggc agctgaccgt
    11821 cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc ccaggatggt
    11881 tcgctttctg gtcctggtat ggcttgtacc acagtcgcca aacagactta cgccctgcct
    11941 gcacctcccg atttaagcgg tggcgcggga acaagttcag tgtctggccc gcagggtgac
    12001 attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc
    12061 agtagcggtt tcctcggcgc tccaacgacc ttagcgccta gtgtactgga aggcagcgaa
    12121 ccgacgcctg cgccacagcc cgttgttaca gcttcgtcga caacgcctgc aacctcgcct
    12181 gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc
    12241 ggggccgtaa gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg acagaagttc
    12301 ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct tggcccattc
    12361 gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc ccaattacag
    12421 tcatttatta ctaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa
    12481 tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt tgctatagta
    12541 gggcactttt ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc
    12601 gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct
    12661 gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg
    12721 gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat
    12781 gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca
    12841 atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc
    12901 accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt
    12961 ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc
    13021 atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa cagctacgtt
    13081 aacgcactgg gcctgaaaaa tacccacttc cagacggtac atggtctgga tgctgatggt
    13141 cagtacagct ccgcgcgaga tatggcgctg atcggccagg cattgatccg tgacgtaccg
    13201 aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac
    13261 cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact
    13321 gacaaagcag gttacaatct tgttgcttct gcgactgaag gccagatgcg cttgatttct
    13381 gcggtgatgg gtggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc
    13441 tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga gttcgcctct
    13501 gaaccggttt ggtttagtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac
    13561 ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct gaacagcagt
    13621 gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat
    13681 ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc
    13741 ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaaatt
    13801 gaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa aaactcccgc
    13861 cttctggcgg gagttgctat ttaattacgt tacgccggag ctgacatgaa aaccaaactt
    13921 aacgaactgc ttgaattccc tactcctttt acttacaaag ttatggggca ggcgttacct
    13981 gagctggttg atcaggtggt tgaagtggta cagcgccatg cgccaggtga ctacacccca
    14041 acggtaaaac caagcagcaa aggcaactac cactcggtat ctatcactat caacgccact
    14101 catatcgagc aggttgaaac actgtatgaa gaactgggca aaatcgatat tgtccgcatg
    14161 gttctgtaac tcgcttctcc gttacccggc tcctggtcgg gtaactcccc tctcctcgct
    14221 gtgatatact tttcccccac ttttactcat tctccacgga gatgccgttt tgtatcagga
    14281 taaaattctt gtccgccagc tcggtcttca gccttacgag ccaatctccc gggctatgca
    14341 tgaattcacc gatacccgcg ataatagtac ccttgatgaa atctggctgg tcgagcacta
    14401 tccggtattc acccaaggtc aggcaggaaa agcggagcac attttaatgc cgggtgatat
    14461 tccggtgatc cagagcgatc gcggtgggca ggtgacttat cacgggccgg gacaacaggt
    14521 gatgtatgtg ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt
    14581 gcttgagcaa acagtggtga ataccctggc tgaactgggt atagaagcgc atcctcgggc
    14641 tgacgcgcct ggtgtctatg tcggggaaaa gaaaatttgc tcactgggtt tacgaattcg
    14701 acgcggttgt tcattccacg gtctggcatt aaacgtcaat atggatcttt caccattttt
    14761 acgtattaat ccttgtgggt atgccggaat ggaaatggct aaaatatcac aatggaaacc
    14821 cgaagcgacg actaataata ttgctccacg tttactggaa aatattttag cgctactaaa
    14881 caatccggac ttcgaatata ttaccgctta attccataca tcaatggccc aatttacact
    14941 gggtcattac tcataatacc tgctaatgct ttacatctcc ttcgcgtttg tatattttct
    15001 ttatcacttg cagaaatgcg gtgccacgcc ttgctccgta actcacccca ttgattaacc
    15061 tattgaaata gttgattatg gtgagcaaaa gcgttccgtt ccaattagac ataagattat
    15121 tgtaattttt taaatattat tttcccatga ataaaattgg agtaagtgcg tggatagtaa
    15181 taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac
    15241 aaccttacga aatattgatc ttaaccttct gactattttt gaagctgtat atgtacataa
    15301 agggatcgtt aatgcagcga aagtgcttaa tctgaccccc tcggcaatca gtcagtctat
    15361 tcagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc agggtgtcac
    15421 tcctaccgca tttgcgatgc atctacatga gtatatcagt cagggccttg agtccattct
    15481 tggcgcgctg gatatcgaag gaagctatga taagcaacga acgataacta ttgcgaccac
    15541 tccctcggtc ggagccctgg tccttcctgt catctatcgg gcgattaaaa ctcactatcc
    15601 gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca gtcagtttca
    15661 aaccgatctc atcatcgata acatgttttg caccaatcgt acggtgcaac atcatgttct
    15721 gttcaccgac aatatggtgt taatttgccg caagggaaat ccactactct ctttagaaga
    15781 tgacagagag actatcgaca acgctgcgca tgtacttctg ttaccggaag ggcaaaattt
    15841 cagcggtctg cggcagagag ttcaagagat gtttccggac cggcaaatca atttcaccag
    15901 ctacaacatt ttgacaatcg ctgcactggt tgccaacagt gacatgttag cgattattcc
    15961 cagccgtttt tataacctgt ttagtcgttg ctggccgctg gaaaaactgc cttttccgtc
    16021 cttaaatgag gagcaaatag atttctctat ccactacaac aaattcatcc tgcgtgatcc
    16081 catcctgcac ggagtaatag atgttatccg taatgcattt tgaaaaagta ttgttacagc
    16141 cagatcacag cgcagaacga taaaatggca caatccaaca acaattttac attttcgcga
    16201 ccgctttggc tgctttcagg tccgtttcaa tgatatactg ccagtcgtta attcaaaaat
    16261 agttgataat tacaacaatc tattgaattg aaacgctttc cttcgtaatt cgcaactgga
    16321 acacgcacgc tatgagtaaa cccattgtga tggaacgcgg tgttaaatac cgcgatgccg
    16381 ataagatggc ccttatcccg gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca
    16441 agccggaatg gatgaaaatc aagcttccag cggactctac acgtatccag ggcatcaaag
    16501 ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga agcctcctgc cctaacctgg
    16561 cggaatgctt caaccacggc acagcaacgt ttatgatcct cggcgctatt tgtacccgcc
    16621 gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc tcctgatgcc aatgaaccag
    16681 tgaaactggc gcagaccatt gccgatatgg cgctgcgtta tgtggttatc acctccgttg
    16741 accgtgatga cctgcgcgat ggcggtgccc aacactttgc ggattgtatt actgccattc
    16801 gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgc ggtcgaatgg
    16861 atcgtgctct ggatattctg actgcaacgc ccccagatgt gttcaaccat aacctggaaa
    16921 acgtaccgcg tatttaccgg caggtacggc ctggcgcaga ttacaactgg tcgctgaagc
    16981 tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac caagtctggt ctgatggtgg
    17041 gtctgggtga aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg
    17101 tgacgatgtt aacgctgggg caatatttgc agccaagccg ccatcacctg ccggttcaac
    17161 gttacgttag cccggatgag ttcgacgaaa tgaaagccga agcgctggcg atgggcttta
    17221 cccatgctgc atgcggtccg tttgtccgct cttcttacca cgccgatttg caggcgaaag
    17281 gaatggaagt taagtaatcc tgtaataatt acttacatct gtctgataaa aaacccgctt
    17341 tttgaagcgg gttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
    17401 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
    17461 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
    17521 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
    17581 ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
    17641 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
    17701 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
    17761 ttgtttaaga tgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
    17821 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
    17881 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
    17941 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccacgaacc
    18001 caggcggcag gaagtaccag gatttcagct ccctgtaaag cctgtgccag cgccagctct
    18061 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccttccac ctccagtaac
    18121 ggagctattt cattaccagc atcaacatgg cgtgattcct gaatggcaaa tgcatcatag
    18181 agatgcagtt tggcataacg ggcgacgatg tttcctgcct gaagtgccac cagcatattc
    18241 catgcgcgcc ccggcgttga aggaagatga atcgtcagaa ttgtcgtcat catgttacgt
    18301 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
    18361 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac aaacagcgat
    18421 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
    18481 acagatgtaa cagcaaactg tcctgcagca accagcattg ccacctcctg tcagagtcgt
    18541 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
    18601 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
    18661 agcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
    18721 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
    18781 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
    18841 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
    18901 ctgggcatta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
    18961 attctggctg ttttcggcct caaccgtaca ctaaagacca aaaaaaaccc gctgattaag
    19021 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
    19081 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
    19141 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
    19201 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
    19261 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttatcagta caccaattgt
    19321 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
    19381 ttgcttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
    19441 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
    19501 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
    19561 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
    19621 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
    19681 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
    19741 acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
    19801 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
    19861 ctgttgccag gtttgtgcaa tattttctct aaacgttgtc atcctctcat ctgcgttagc
    19921 aaaaacttta ccaacgctga ttaactgaat aaaaacaaag gaaaagatag cgacatattt
    19981 actcacgttc atttgtgacc ataaaacatt tatcaaaaat ctactactag catagcaaag
    20041 ctacaattaa cataacctta atagacccaa cataaagaat aatctgaata gctgctctat
    20101 ttatttccgc ttgttcaagt taaatatttc cgcgcatcga ttaaagatga tgcgtcgttt
    20161 tctgcgatgg gaatagtcaa aaaagaaaaa ccaagtcttt tttgatgaca aaatgcaatc
    20221 aaggaaaaat tattttattt ttttaacgag atacaacaat catcttaacg aagtatataa
    20281 tatatatcgt caaccatgct catattagat tattttttga gcgcacagcc gcagctaaca
    20341 ccattgcaat taacaaattt gcatcaatcc accatcaatt tacacacatt attatgtgat
    20401 aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagca acttcaatgt
    20461 cttaatggca gtttttcttg attttaatca gcattcatcg ccaatttatt gggcatattt
    20521 tttccttaag ctttaggaag tttttattat ttactttggg cctggagaca ggaaaaatta
    20581 tgctgacatt cattgaactc cttattgggg ttgtggttat tgtgggtgtg gctcgctaca
    20641 tcattaaagg gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta
    20701 tcagtgccat tatggggcac aaagtgttac cgtccagcca ggcttcaaca ggctacagcg
    20761 ccacggatat cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca
    20821 tgatgattat gatgctgtgt ggctttgccg cttacatgac ccatatcggc gcgaatgata
    20881 tggtggtcaa gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga
    20941 ttgccgccta ttttgttgct tgtctgatgt cactggccgt ctcttccgca accggtctgg
    21001 gtgttttgct gatggcaacc ctgtttccgg tgatggtaaa cgttggtatc agtcgtggcg
    21061 cagcagctgc catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg
    21121 tggtgctggc ggcgcaggct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc
    21181 tgcctatctc aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt
    21241 atctggataa aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca
    21301 ctgcccccgc gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg
    21361 acggtaaatg gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg
    21421 cctccattct ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag
    21481 tggcttatcg cgggatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg
    21541 gggtattcgc tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta
    21601 cctcgtttgg ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg
    21661 cggcagtcac gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga
    21721 aactggcgca ctcttccggc attaacccgg cgtatttgac tatcccgatg ctgcaggcgt
    21781 caaacctggg tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg
    21841 cgaaaatctc accatttgaa gtcgtaaaac gcacctcggt gccggtgctt gttgggctag
    21901 tgattgttat cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacagaca
    21961 agtaaatagt aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta
    22021 tatgcgttgt ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct
    22081 ggcgcaatat tcaagataac gcctggcagt ggtgcggctg atggtcagtg cctgcgccac
    22141 cgtttctgcc gtatgttgca caccaggctc tttaaacagt tttcgcaccg cgtttagcgt
    22201 caaggggtca atgccggtcg gcagttcgtc tttgggttca ccgcgagcat aggcattaaa
    22261 catctcatca atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg
    22321 gcggaaacgg gttagcgttt gccccagtgt attgattcac ttgaagtacg aaaaaaccgg
    22381 gagaacattg gattattcgg gatctgatgg gattagattt ggtggggctt gcaagcctgt
    22441 agtacaaatt ttaggcgtta accaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt
    22501 tcattgaaaa aacgaaaaac aaaaacacta taaatcattt caatcatagc ttcaaaaaac
    22561 gttcaaaacc gataacaacc aggctgtaac caaatgactc atatcacaaa tcagctcatg
    22621 ccgtttaggt atgttacatg tgtgattatg tgaggtgaag tatgttttag ctggctcatg
    22681 gttgttatac ggcttttttc gcatcttgtg gttccaacaa aggagctaca ggctcttcct
    22741 gtttatgaac accatacatt gacaaaatct cttggcgagt ttctggaggt aatttatgca
    22801 gcgccaaagc tgtttgtagt gtttggcgat caatacccag cgttgccaat agggtgtcaa
    22861 cggagatgga ggggcgttct tgcattaatc cggaaagccc atatttagcc atgtttagct
    22921 ctatctctcg gacttcattt tctcctagtt tattgattaa cagggagaac attttcaatg
    22981 tatcagcatt catttcttcg atagcaattt tttgcatagc cggtattgcc gggatatatt
    23041 caaccccagg cccgcgacgc ccttcttgtg aacgcttcgg ccatccttct ttctttgctc
    23101 gtttatgcac cccctgtatt gtcccaggca ttccatccag ttcagtaatt tgtttgggtg
    23161 tcatccactg gtttatctca tttgcgcttt tcataaacta agtttaccag atttatctat
    23221 ataaaaatca atggattaca aaataaacca cattaactga tataaaccgg ttttgtttat
    23281 ttggtaatgt ataaaacaga taatcaagac gaattatttg ttttggtaac cctttatcat
    23341 acgcaatatg gttggtgtgc gaaagatgag taacgattgg catcgggccg acgtgattgc
    23401 ggccctcaaa aaaacaggaa cccctctggc aaaattgtcc agacgttcag gttattcaac
    23461 cgggacgctt gcaaatgcat tagtcaggcc atggcctaaa ggtgaacgca ttattgctga
    23521 cgccttaaac atgaaacctg aggaaatttg gcctagtaga tatctaaggg atcacaatga
    23581 gagagtggat atcagcaatt gaatgcatgg gtttatctgg tttacctggt tcattaatgg
    23641 gggttcacaa aagagctcag agagaaggat ggctcaagag aagaagagaa ggaattagag
    23701 gggttgctta cgaataccat atatcttctc ttcctaacat tgctcgagct gaagttttgc
    23761 tccaacaagg aaaatttaaa accagtcagg gatgttttga aatcgcccgc cccacgctgg
    23821 aagctcatga ttatgatcgt gaggcactat ggagcaaatg ggataaagcc agcgattccc
    23881 agcgcagcct ggctgaaaaa tggttgccct caattcaggc cacagacgaa atgctgaacc
    23941 aggggatttc aacgaaaacg gcttttgcga ccgttgcagg gcattaccag gtcagcgcat
    24001 ccactttgcg ggacaagtat taccaggtac agaagtttgc gaagcctgac tgggcggctg
    24061 cacttgttga tggacgtgga gcatcccgtc gcaatgttca caaaagtgaa tttgacgagg
    24121 atgcctggca gtttctgatt gcagattatc tgcgaccgga aaaacccgcc ttccgcaaat
    24181 gttatgagcg tctggaactg gcagcccgcg agcatggctg gagtattccc tcccgtgcca
    24241 cggcctttcg ccggattcag caactggacg aggcaatggt tgttgcctgt cgtgaaggtg
    24301 aacatgcact gatgcatctg ataccggcac agcagcgaac tgtggaacac ctggacgcca
    24361 tgcagtggat caacggcgac ggttatctgc ataacgtctt tgtacgctgg tttaacggtg
    24421 atgtgatccg tccgaaaaca tggttctggc aggatgtgaa aacccgaaaa attctgggct
    24481 ggcgctgcga tgtgagcgag aacattgatt caattcgcct ctcgttcatg gatgttgtga
    24541 ctcgctacag tatcccggag gattttcaca tcaccattga taacacccgt ggtgctgcga
    24601 ataaatggct gacgggaggc gcgcccaatc gctaccgctt taaggtaaaa gaggacgatc
    24661 caaaaggact gtttttactg atgggtgcga aaatgcactg gacaagcgtt gttgccggta
    24721 aaggctgggg ccaggcaaaa cctgttgaac gtgctttcgg tgttggtggg cttgaggaat
    24781 acgttgataa gcatccggca ctggctggcg catatacggg gccaaatccg caggcaaaac
    24841 ctgataacta tggcgaccac gctgttgatg cagagctgtt tctgaaaacc tttgccgaag
    24901 gtgtggcgat gttcaatgcc agaacaggcc gtgaaacaga aatgtgcggg ggcaaactct
    24961 cgtttgatga tgttttcgag cgtgaatacg ccagaacgat tgtgcgtaag ccaaccgaag
    25021 aacaaaaacg gatgctgtta ctgcctgccg aggcggtgaa cgtttcacgc aaaggcgagt
    25081 ttacgcttaa agttggcggc tcccttaaag gcgcgaaaaa cgtttattac aacatggcat
    25141 taatgaatgc cggagtgaaa aaagttgtgg tcagatttga tccacagcag ttacacagca
    25201 cggtttattg ag
//
LOCUS       DAEBXD010000054        24954 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEBXD010000054 DAEBXD010000000
VERSION     DAEBXD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24954
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.054"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(50..274)
                     /gene="cspD"
                     /locus_tag="KJL94_002352"
     CDS             complement(50..274)
                     /gene="cspD"
                     /locus_tag="KJL94_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBD4410680.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            597..917
                     /gene="clpS"
                     /locus_tag="KJL94_002353"
     CDS             597..917
                     /gene="clpS"
                     /locus_tag="KJL94_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBD4410681.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            948..3224
                     /gene="clpA"
                     /locus_tag="KJL94_002354"
     CDS             948..3224
                     /gene="clpA"
                     /locus_tag="KJL94_002354"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBD4410682.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(3627..3714)
                     /locus_tag="KJL94_002355"
     tRNA            complement(3627..3714)
                     /locus_tag="KJL94_002355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3678..3680),aa:Ser,seq:gga)
     gene            complement(3968..4186)
                     /gene="infA"
                     /locus_tag="KJL94_002356"
     CDS             complement(3968..4186)
                     /gene="infA"
                     /locus_tag="KJL94_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBD4410683.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4471..5175)
                     /gene="aat"
                     /locus_tag="KJL94_002357"
     CDS             complement(4471..5175)
                     /gene="aat"
                     /locus_tag="KJL94_002357"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBD4410684.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5217..6938)
                     /gene="cydC"
                     /locus_tag="KJL94_002358"
     CDS             complement(5217..6938)
                     /gene="cydC"
                     /locus_tag="KJL94_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBD4410685.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6939..8705)
                     /gene="cydD"
                     /locus_tag="KJL94_002359"
     CDS             complement(6939..8705)
                     /gene="cydD"
                     /locus_tag="KJL94_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBD4410686.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8828..9793)
                     /gene="trxB"
                     /locus_tag="KJL94_002360"
     CDS             complement(8828..9793)
                     /gene="trxB"
                     /locus_tag="KJL94_002360"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBD4410687.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10337..10831
                     /gene="lrp"
                     /locus_tag="KJL94_002361"
     CDS             10337..10831
                     /gene="lrp"
                     /locus_tag="KJL94_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBD4410688.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10966..14994
                     /gene="ftsK"
                     /locus_tag="KJL94_002362"
     CDS             10966..14994
                     /gene="ftsK"
                     /locus_tag="KJL94_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBD4410689.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            15149..15760
                     /gene="lolA"
                     /locus_tag="KJL94_002363"
     CDS             15149..15760
                     /gene="lolA"
                     /locus_tag="KJL94_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBD4410690.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15771..17114
                     /gene="rarA"
                     /locus_tag="KJL94_002364"
                     /gene_synonym="mgsA"
     CDS             15771..17114
                     /gene="rarA"
                     /locus_tag="KJL94_002364"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBD4410691.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17205..18497
                     /gene="serS"
                     /locus_tag="KJL94_002365"
     CDS             17205..18497
                     /gene="serS"
                     /locus_tag="KJL94_002365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBD4410692.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            18737..21181
                     /gene="dmsA"
                     /locus_tag="KJL94_002366"
                     /pseudo
     CDS             18737..21181
                     /gene="dmsA"
                     /locus_tag="KJL94_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            21192..21809
                     /gene="dmsB"
                     /locus_tag="KJL94_002367"
     CDS             21192..21809
                     /gene="dmsB"
                     /locus_tag="KJL94_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD4410693.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            21811..22602
                     /locus_tag="KJL94_002368"
     CDS             21811..22602
                     /locus_tag="KJL94_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD4410694.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(22638..23264)
                     /locus_tag="KJL94_002369"
     CDS             complement(22638..23264)
                     /locus_tag="KJL94_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD4410695.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            23579..24727
                     /locus_tag="KJL94_002370"
     CDS             23579..24727
                     /locus_tag="KJL94_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410696.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         6222 a         6161 c         6643 g         5928 t
ORIGIN      
        1 gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg
       61 ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga
      121 catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg
      181 aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg
      241 cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat
      301 acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct
      361 agaaatacaa tttagagaat tagggagagc cgtcaagcat ttgacagggg ccagggggca
      421 ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct
      481 gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc
      541 tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg
      601 gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc
      661 taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt
      721 ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga
      781 tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag
      841 aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta
      901 cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag
      961 aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta
     1021 tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg
     1081 aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa
     1141 ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc
     1201 agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg
     1261 gtgcaaacgt tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc
     1321 gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag
     1381 acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg
     1441 gggaggaacg tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa
     1501 tcgacccact aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc
     1561 gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag
     1621 aaggtcttgc ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga
     1681 tttactctct cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa
     1741 aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg
     1801 atgagatcca caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta
     1861 acctaatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc
     1921 aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg
     1981 atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt
     2041 atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg
     2101 cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag
     2161 cgggcgctcg cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg
     2221 atattgagtc cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg
     2281 accgcgatac cctgaaaaaa ctcagcgatc gcctgaaaat gctggtcttc ggccaggata
     2341 aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac
     2401 ataaaccggt cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga
     2461 cggtacagct ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata
     2521 tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc
     2581 agggcggttt gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg
     2641 aaatcgagaa agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta
     2701 cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca
     2761 ctaacgccgg ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca
     2821 gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg
     2881 acaacattat ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat
     2941 tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg
     3001 aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg
     3061 cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc
     3121 tggtggacgg cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg
     3181 gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag
     3241 gttggtgaag tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg
     3301 tgaaaaccct atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta
     3361 aggataaaaa agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa
     3421 acgtgtagta tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa
     3481 aacctttgcg aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact
     3541 ccacccgccc accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca
     3601 ctttcgtacg agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg
     3661 ttgccgtata cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg
     3721 ttttgctacc aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac
     3781 ggtcaagaag aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt
     3841 atatctacat cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa
     3901 aataaaaagg ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt
     3961 aaaacaatca gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt
     4021 caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga
     4081 tgtgtgcagt aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg
     4141 tttcaagaac ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg
     4201 tatcactacc gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat
     4261 ttgcgcgcta aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc
     4321 cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc
     4381 gaaaaattcc ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata
     4441 acactctggg gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg
     4501 tacccagaaa ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc
     4561 ccggcgggga atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc
     4621 gataagctta ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga
     4681 cgcattttcc atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc
     4741 gtacataccg ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag
     4801 ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg
     4861 atcgctggca cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg
     4921 cgagcgttta tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac
     4981 cgcgcgggga tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat
     5041 accacgctgg taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag
     5101 gccgttaggc tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag
     5161 ctgaaccaag cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac
     5221 aaaccctgct tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct
     5281 tgctcaataa tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga
     5341 agtcgatggg tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg
     5401 atctggcttt cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc
     5461 gcatcatgta acagcgtacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg
     5521 cgtccgcctt cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca
     5581 acgcgacgca agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga
     5641 ttatcacgca gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt
     5701 cgtagagccg cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt
     5761 tgcgggtccc atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc
     5821 ccgagaatcg ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt
     5881 tgggattgct ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca
     5941 acacgagttt gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata
     6001 cgtacggcag aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt
     6061 gcttcaaacg cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca
     6121 ttgccgccaa cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg
     6181 agcagcatta tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc
     6241 cattgaattt ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc
     6301 agctcagctt gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga
     6361 gtcagatttt gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc
     6421 gtcagtaaca taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg
     6481 attgtcacca ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa
     6541 agatgatcga gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag
     6601 cgcgccagtc cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc
     6661 tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta
     6721 cgggtgattg cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt
     6781 ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg
     6841 atactggcga gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga
     6901 cgtttataca gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg
     6961 gccagtaatg tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca
     7021 ataatccggc catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg
     7081 tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc
     7141 tgttcactgt gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga
     7201 tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc
     7261 tggtcgccaa caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag
     7321 gcgttatcca gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg
     7381 ttatcccgca attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta
     7441 cgccatgatt ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga
     7501 tatgagagaa aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca
     7561 accaacactg cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta
     7621 ccttccggcg acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct
     7681 aattccgcct caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg
     7741 tcagctgcac caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc
     7801 tggaaaaact ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta
     7861 tcgtagtgac caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga
     7921 gcaattgaca gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc
     7981 acttccattg tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct
     8041 tcaccacgac caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca
     8101 cttaagcgag caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc
     8161 agcgccataa acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag
     8221 ttagagggga agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc
     8281 ggcagatagc gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc
     8341 cagctccccg caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga
     8401 acctgacggc ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt
     8461 aaccagacca cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc
     8521 agggcttcac ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag
     8581 gcctgggcaa tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa
     8641 cgttgggaga tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta
     8701 ttcattgcac gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg
     8761 gaaaaaataa aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt
     8821 tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca
     8881 tgcagccagt accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg
     8941 cggcaaagac gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt
     9001 tgatgtagcc gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac
     9061 cgatagcaac aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat
     9121 cgcgcagacg aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt
     9181 tggtgtgcag aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt
     9241 tttccgcgcg gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca
     9301 gatacagcgc ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc
     9361 gatagaagaa accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt
     9421 cagagggcag gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag
     9481 tgtattcggc gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga
     9541 tatgatcaaa aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta
     9601 ataacggacc ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg
     9661 tcagttggcc gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg
     9721 cgtagacagc agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt
     9781 tggtcgtgcc catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat
     9841 ttacagacgt aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc
     9901 gataagcagg tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat
     9961 aagccgatta aatgatgaat aaacgccctt gttaatgaat atctggcatg ttgtactaaa
    10021 aatcgatgtt ttgctttgac aatcccctag tgttttgcga aaacattcga ggaagaaaaa
    10081 aacagtattc ttatatgcgc ataaccatgt atgtaaatac catgtttacc gtgctagtga
    10141 aatctacgta tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg
    10201 gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg
    10261 tgacgggtag cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata
    10321 cagagagaca ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat
    10381 cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc
    10441 taaacgtgtg ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca
    10501 agggtttatt cagggctata cggcgctgct taacccccat tatctggatg catcacttct
    10561 ggtattcgtt gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac
    10621 cgctgtacaa aaacttgaag aaattcagga gtgtcattta gtatctggtg atttcgacta
    10681 cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt aagttgctgg gggaaaccct
    10741 gctgcgtctg cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca
    10801 gagtaatcgt ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt
    10861 tgattacact cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc
    10921 gttttagcat cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac
    10981 actgaagaca aagaagtcac attgacaaag ttaagtagcg gccgccgcct tctggaggca
    11041 ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagctttaac
    11101 ccttcggacc ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg
    11161 atgcccggtg cgtggttggc agatacgctg ttctttattt ttggcgtgat ggcttacacc
    11221 attcccgtca ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa
    11281 tacattgatt attttgccgt ttcgctacgc atcattggcg ttttggcgct catccttacc
    11341 tcctgtggtc tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc
    11401 attggcagct tactaagcac tacgctacaa ccactgctac acagtagcgg gggaactatt
    11461 gcgctgctct gcgtttgggc agcgggcctg acgttgttca ccggttggtc atgggtgacc
    11521 attgctgaaa aactcggcgg ctggatttta aacattctca ccttcgccag taatcgtacc
    11581 cgtcgcgatg atacctgggt cgatgaagat gagtatgaag acgacgaaga gtatgaagat
    11641 gaaaatcacg gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg
    11701 cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg
    11761 ttgttctccg gtaagcggat ggatgatgac gaagagatta cctacactgc acgcggtgtg
    11821 gctgccgacc cggacgacgt cctattttcg ggcaatcgtg caacgcagcc agaatatgac
    11881 gaatacgatc cattattaaa cggtgcgcca attaccgaac ctgtcgctgt agcagctgct
    11941 gctaccacgg cgacacaaag ctgggctgcg ccggttgaac ctgtgactca gacgccgcct
    12001 gttgcctctg ttgatgttcc accttcgcaa cctacagtag cctggcagcc tgtaccgggt
    12061 ccacaaacgg gagagccggt tattgctcct gcaccggaag gttacccaca gcagtcacaa
    12121 tatgcgcagc ctgcagtgca atataatgag ccgctgcaac aaccagtaca gccgcagcag
    12181 ccgtattatg cacctgcagc tgaacaacct gcgcaacagc cgtattatgc ccctgcgcca
    12241 gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc agcaatccac ttttgctcca
    12301 cagtctacat accagactga gcaaacttat cagcagccag ccgctcagga gccgttgtac
    12361 caacagccgc aacccgttga acagcagcct gttgtggagc ctgaacccgt tgtagaagag
    12421 acaaaacccg cgcgtccgcc gctttactat tttgaagaag tggaagagaa gcgagcccgt
    12481 gaacgtgaac aacttgcggc ctggtatcaa ccgattccag aaccggttaa agaaccagaa
    12541 ccgatcaaat cttcgctgaa agcaccttct gttgcagcag tacctccagt agaagccgct
    12601 gccgctgttt ctccgctggc atctggcgtg aaaaaagcga cactggcgac gggggctgcc
    12661 gcaaccgttg ccgcgccagt cttcagtctg gcaaatggcg gtggaccgcg tccgcaggtc
    12721 aaagagggga ttggcccgca gttgccacga ccgaaacgta tccgcgtgcc aactcgtcgt
    12781 gaattggcgt cttacggtat taagctgccc tcacagcgtg cggcggaaga aaaagcccgt
    12841 gaagcccagc gcaatcagta tgattctggc gatcagtata acgatgatga aatcgatgcg
    12901 atgcagcagg atgaactggc acgtcagttc gcccagacac agcagcaacg ctatggcgaa
    12961 cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag atgctgcggc agaggctgaa
    13021 ctggctcgtc agtttgtgca aactcaacaa caacgttatt ccggcgaaca accggctggg
    13081 gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt gctggatgat
    13141 ggtccacacg aaccgctgtt tacgccaatt gttgaacctg tacagcagcc gcaacaaccg
    13201 gttgcaccac agcagcagta tcagcagcca caacagccgg ttgcgccaca gccgcagtat
    13261 cagcagccac aacagcaggt tgcgccgcag ccacaatatc agcagccgca acaaccggtt
    13321 gcgccacagc agcaatatca gcagccgcaa caaccagttg cgccgcagcc gcagtatcaa
    13381 cagccgcaac aaccggttgc accgcagccg cagtatcagc agccacaaca gccagttgcg
    13441 ccacagccgc aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg
    13501 ttgcacaaac cgacgacgcc gctgccttct ctggatttgc tgacaccgcc gccgagcgaa
    13561 gtggagccgg tagatacctt tgcgcttgaa caaatggcgc gtctggtgga agcgcgtctg
    13621 gctgatttcc gtattaaagc cgatgtcgtt aattactctc cggggccagt tatcactcgc
    13681 tttgaactta acctggctcc tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac
    13741 cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc
    13801 tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg
    13861 gataacgcca aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc
    13921 gccggtgagc cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact
    13981 accggttccg gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca
    14041 cagccagaag atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat
    14101 gaaggcattc cgcacctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg
    14161 ctgcgctggt gtgttaacga gatggagcgt cggtataaac tgatgtctgc gctgggtgtg
    14221 cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt
    14281 ccagacccgt actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa
    14341 gaaccataca ttgtcgtgtt ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa
    14401 aaagtggaag agctgatagc tcgtctggcg caaaaagccc gtgccgcggg tatccacctc
    14461 gtactggcaa ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt
    14521 ccgacccgta tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag
    14581 gctggcgcgg aatcactgct gggtatgggg gatatgctct actctgggcc gaactccacg
    14641 ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag
    14701 gactggaaag cgcgtggtcg cccacagtat gttgatggca tcacctccga cagcgaaagc
    14761 gaaggtggtg cgggtggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg
    14821 gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc
    14881 attggttata accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc
    14941 gaacaggggc acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg
    15001 cattgccgga taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta
    15061 ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat
    15121 cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gctatcacct gtgcattact
    15181 ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct
    15241 ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc
    15301 ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca
    15361 tatgacacaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa
    15421 cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt
    15481 tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg
    15541 cgatgacttt gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa
    15601 cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag
    15661 cagttatcag ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac
    15721 cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc
    15781 tgtcgctcga tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa
    15841 atttagcaca gtatatcggt cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg
    15901 ctatcgaagc cgggcatttg cattctatga tcctctgggg gccaccgggt accggcaaaa
    15961 caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg
    16021 tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg
    16081 caggtcgccg cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg
    16141 atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa
    16201 acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtttat ctgttgaaat
    16261 ccctgagtac agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg
    16321 gctatggtgg tcaggatatt gttctgccag atgatacacg acgtgccatt gcagaactgg
    16381 tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag
    16441 tctatgatag cggtaagcgg gtcctgaagc ctgaattact gatcgaaatc gccggtgaac
    16501 gtagcgcccg ctttgataac aaaggcgatc gcttttacga tctaatttcc gcactgcata
    16561 agtcggtacg tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga attattaccg
    16621 ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc gattgcgtct gaagacgtcg
    16681 gtaatgccga tccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg
    16741 ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc
    16801 caaaaagcaa cgctgtctac actgcgttta aagccgcgct ggccgatgct cgcgaacgcc
    16861 cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg
    16921 gctacgggca ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg
    16981 tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg
    17041 aaggcaagat tggtgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa
    17101 aacgctaccg ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg
    17161 ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc
    17221 tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg
    17281 atgtagataa gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa
    17341 acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg
    17401 aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa
    17461 aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc
    17521 tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagccgct
    17581 ggggtacccc gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc
    17641 actctggcct cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga
    17701 aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg
    17761 aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg
    17821 gtacgggtca gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag
    17881 aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg actaacctgg
    17941 tgcgcggtga aatcatcgat gaagatgatc tgccaattaa gatgatcgcc cacaccccat
    18001 gcttccgttc tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc
    18061 agttcgacaa agttgaaatg gtgcagatcg tgcgcccaga agactcaatg gcggcgctgg
    18121 aagagatgac tggtcatgca gaaaaagtcc tgcagttgct gggcctgccg taccgtaaaa
    18181 tcatcctttg cactggcgac atgggctttg gcgcttgcaa aacttacgac ctggaagtat
    18241 ggatcccggc acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc
    18301 aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc
    18361 ataccctgaa cggttctggt ctggctgttg gtcgtacgct ggttgcagta atggaaaact
    18421 atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac
    18481 tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgccttt agggcgcttt
    18541 ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc
    18601 gagtgaaaat ctaccgatct ctttgatttt caaattattc tatgtataca agcctatata
    18661 gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgatcaa
    18721 gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggagag
    18781 tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt
    18841 aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcaga
    18901 cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat
    18961 gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacggac aataccggcg atgacaatta
    19021 cgacggccta cactaggttc gcgcctgcct gcgtgggcgt tccatgcgtc accgtgtcta
    19081 caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt
    19141 cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat
    19201 caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc tgggcggcac
    19261 catgacccgc tcctggccgc cgggaaatac cctggtcgcg cgcctgatga actgctgcgg
    19321 cggttatctt aaccattacg gtgactactc ttccgcgcaa attgcggaag gtttgaacca
    19381 tacctacggc ggctgggcag atggtaacag cccgtcggat atcgaaaaca gtaaattggt
    19441 agtgctgttt ggtaataacc caggcgaaac gcgaatgagt ggcggtgggg tgacttacta
    19501 tcttgaacag gcacgccaga aatctaatgc tcgcatgatc atcatcgatc cgcgctatac
    19561 cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccaggaa cagatgccgc
    19621 actggttaac ggtctggcgt acgtcatgat cactaaaaac ctggtggatc aggcattcct
    19681 cgataaatat tgcgttggct atgacgagaa aaccctgccc gccagtgcgc cgaaaaatgg
    19741 tcattataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa caccggaatg
    19801 ggcctcgcaa atcaccggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg
    19861 cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga
    19921 aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaatgg
    19981 agggaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgacctt
    20041 ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg
    20101 cccggaaatg acggcgctgc gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat
    20161 caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg
    20221 tacccatgaa atcctccagg atgataagaa gtgcgagctg attgtggtta tcgactgcca
    20281 catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca
    20341 gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca
    20401 ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa
    20461 acgtcttggc gttgagcaac agtttactga aggccgtacc taggaagagt ggatgcggca
    20521 tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa
    20581 gcaggggatc tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg
    20641 tgaagatccg caggcaaacc cactgactac gccatcgggc aaaattgaga tttattcgca
    20701 ggcgctggct gacattgccg ctacctggga attgccagaa ggcgatgtga tcgatccact
    20761 gccgatctac acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct
    20821 gcagcttaca ggtttccact ataaatctcg cgttcactca acttacagca acgttgatgt
    20881 gctgaaagcg gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg
    20941 tatccacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc
    21001 gaaagtgacg ccacgaatga tgccgggcgt ggtcgcactg ggtgaaggcg cctggtatga
    21061 cccggatgca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca ctcaacgtcc
    21121 gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta
    21181 aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt
    21241 gcaaaacctg cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagtttcc
    21301 gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga
    21361 acgtgtttgc ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta
    21421 aagtctgccc gagcggtgcg atgcataaac gcgaagatgg ttttgttgtg gtcgatgaag
    21481 atgtatgcat tggctgccgt tactgtcata tggcttgccc gtatggcgca ccgcaatata
    21541 acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggta
    21601 aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct ggatttcggc cctatcgacg
    21661 agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta
    21721 ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg gataccactg
    21781 gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg
    21841 atgatcttca cggtcttcgg gcaatgtgtg gcgggtggtt ttatcgttct ggctttggcg
    21901 ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg catgtttggc
    21961 ttatgggtac tgatgggcat tggctttatc gcctctatgc tccatcttgg ttcaccgatg
    22021 cgcgccttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc
    22081 ggttcgatct tttttgctgt aggcggcatc ggctggttgc tggcaatgct gaaaaagctg
    22141 tcaccggcat tgcgtacgct atggctggtg gtgacgatgg ttcttggcgt cgtctttgtc
    22201 tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg
    22261 acgccgatgg gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg
    22321 agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag
    22381 caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcggat cgtgcttttg
    22441 gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg
    22501 ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc
    22561 tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt
    22621 tcggccccgg ttctctttta tttctgcttc gttaacgtgt cataactggt catcaaatta
    22681 cgataatcag gaatatggtt agagaacaac gtcgccagtc cttcaatatc attacgccag
    22741 tcacggtgca gctcgcaagc cacgccgaac catgtcatca attgcgcacc agcttgcgac
    22801 atgcgatccc acgccgaatg ccgggtaatt tcattaaagg tccctgatgc atcagtcacg
    22861 acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg
    22921 gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa
    22981 tcttcgttat cccaggcgtt aatattgccc gggcgagcaa tgtaaggtgc atcggggaat
    23041 tgtgctttca gttctagaac taatgggccg ttaggaccgg tttcaaaact ggtggtaaga
    23101 atagttggta agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac
    23161 ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctggtgatc aacaagcaaa
    23221 acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga
    23281 gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa gcatagacgg
    23341 gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt
    23401 gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta gcgttatgaa
    23461 tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca
    23521 atgtttttgg cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat
    23581 gtccacgtat acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc
    23641 gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg
    23701 gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac
    23761 agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctgtcct ccttcatttt
    23821 tgctgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt tggcgtggcg
    23881 ttttgtcgcg ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg cgctgatgtg
    23941 cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg tttattacgt
    24001 gggaacgttt ttaggccagt tactggtcag caaagtctca accgagctga tgagcgtatt
    24061 gccgtgggtt acaggtttga cattggcagg gatcttaccg ctgttgttta cgcgtgtcct
    24121 gaatcagcag gctgaaaacc atgattcgac gtcaattacg gcaatgctaa aactccgtca
    24181 ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gttctgggat ctctatatgg
    24241 cctgatgcct ctgtacctca atcacaaagg ggtgagcaat gccagtattg gtttctggat
    24301 ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc tggcggataa
    24361 gtttggtcga ctgctggttt tgcgtgttca ggtctttgtc gtcattctcg gcagtattgc
    24421 gatgcttagc caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac
    24481 gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc aactggtggc
    24541 gatgaaccaa gccttactgt tgagctatac cgtgggaagt ctgcttggcc cgtcatttag
    24601 cgctatgcta atgcagaatt tctccgataa tttattgttt atcatgatcg ccagcgtatc
    24661 gtttatctat ttgctgatgc tgcttcgcaa cgccggtcat acgccgaaac ccgttgctca
    24721 cgtgtaaatg aattcaagca aagtgtgaac ttactgtttc acactctgct tttttgtttc
    24781 ttctatctga cttgcttttt tccaaatttt attcgtttaa aaataaaatg tgcagcaggt
    24841 tataattttg catttcgcta tttccgcact tcttatttgc cgcgcataat ccctcgtttt
    24901 acagatgccc ctttaatttt ggcgaaggat ttgtctatgg ctgggaatgt tcag
//
LOCUS       DAEBXD010000055        24600 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEBXD010000055 DAEBXD010000000
VERSION     DAEBXD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.055"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..374)
                     /locus_tag="KJL94_002371"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="KJL94_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="KJL94_002372"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="KJL94_002372"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBD4410697.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="KJL94_002373"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="KJL94_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBD4410698.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="KJL94_002374"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="KJL94_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBD4410699.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="KJL94_002375"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="KJL94_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBD4410700.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="KJL94_002376"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="KJL94_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBD4410701.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="KJL94_002377"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="KJL94_002377"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBD4410702.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="KJL94_002378"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="KJL94_002378"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBD4410703.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNIGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="KJL94_002379"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="KJL94_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBD4410704.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="KJL94_002380"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="KJL94_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBD4410705.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="KJL94_002381"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="KJL94_002381"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBD4410706.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="KJL94_002382"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="KJL94_002382"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBD4410707.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGITSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="KJL94_002383"
     CDS             10736..11161
                     /locus_tag="KJL94_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBD4410708.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="KJL94_002384"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="KJL94_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBD4410709.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="KJL94_002385"
     CDS             12185..13621
                     /locus_tag="KJL94_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBD4410710.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KJL94_002386"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KJL94_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBD4410711.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KJL94_002387"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KJL94_002387"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBD4410712.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KJL94_002388"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KJL94_002388"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBD4410713.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="KJL94_002389"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="KJL94_002389"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBD4410714.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KJL94_002390"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KJL94_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBD4410715.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="KJL94_002391"
     CDS             complement(20454..20747)
                     /locus_tag="KJL94_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD4410716.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="KJL94_002392"
     CDS             complement(20744..22033)
                     /locus_tag="KJL94_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD4410717.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="KJL94_002393"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="KJL94_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="KJL94_002394"
     CDS             complement(23047..23811)
                     /locus_tag="KJL94_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBD4410718.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="KJL94_002395"
     CDS             24127..>24600
                     /locus_tag="KJL94_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBD4410719.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6096 a         5940 c         6462 g         6102 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatatcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaataaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DAEBXD010000056        24351 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEBXD010000056 DAEBXD010000000
VERSION     DAEBXD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24351
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.056"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(193..765)
                     /locus_tag="KJL94_002396"
     CDS             complement(193..765)
                     /locus_tag="KJL94_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBD4410720.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            983..1843
                     /gene="tesB"
                     /locus_tag="KJL94_002397"
     CDS             983..1843
                     /gene="tesB"
                     /locus_tag="KJL94_002397"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBD4410721.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRSGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1892..3178)
                     /gene="amtB"
                     /locus_tag="KJL94_002398"
     CDS             complement(1892..3178)
                     /gene="amtB"
                     /locus_tag="KJL94_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBD4410722.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3208..3546)
                     /gene="glnK"
                     /locus_tag="KJL94_002399"
     CDS             complement(3208..3546)
                     /gene="glnK"
                     /locus_tag="KJL94_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBD4410723.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3727..5508)
                     /locus_tag="KJL94_002400"
     CDS             complement(3727..5508)
                     /locus_tag="KJL94_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD4410724.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5501..7273)
                     /locus_tag="KJL94_002401"
     CDS             complement(5501..7273)
                     /locus_tag="KJL94_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD4410725.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7303..7761)
                     /gene="decR"
                     /locus_tag="KJL94_002402"
     CDS             complement(7303..7761)
                     /gene="decR"
                     /locus_tag="KJL94_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBD4410726.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7915..8733)
                     /gene="cof"
                     /locus_tag="KJL94_002403"
     CDS             complement(7915..8733)
                     /gene="cof"
                     /locus_tag="KJL94_002403"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBD4410727.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8832..10524
                     /locus_tag="KJL94_002404"
                     /pseudo
     CDS             8832..10524
                     /locus_tag="KJL94_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10589..11284
                     /gene="queC"
                     /locus_tag="KJL94_002405"
     CDS             10589..11284
                     /gene="queC"
                     /locus_tag="KJL94_002405"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBD4410728.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11336..11734)
                     /gene="fadM"
                     /locus_tag="KJL94_002406"
     CDS             complement(11336..11734)
                     /gene="fadM"
                     /locus_tag="KJL94_002406"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBD4410729.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11828..12199)
                     /locus_tag="KJL94_002407"
     CDS             complement(11828..12199)
                     /locus_tag="KJL94_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBD4410730.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12350..14221)
                     /gene="ppiD"
                     /locus_tag="KJL94_002408"
     CDS             complement(12350..14221)
                     /gene="ppiD"
                     /locus_tag="KJL94_002408"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD4410731.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14413..14685)
                     /gene="hupB"
                     /locus_tag="KJL94_002409"
     CDS             complement(14413..14685)
                     /gene="hupB"
                     /locus_tag="KJL94_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBD4410732.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14894..17248)
                     /gene="lon"
                     /locus_tag="KJL94_002410"
     CDS             complement(14894..17248)
                     /gene="lon"
                     /locus_tag="KJL94_002410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBD4410733.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17436..18710)
                     /gene="clpX"
                     /locus_tag="KJL94_002411"
     CDS             complement(17436..18710)
                     /gene="clpX"
                     /locus_tag="KJL94_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBD4410734.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18836..19459)
                     /gene="clpP"
                     /locus_tag="KJL94_002412"
     CDS             complement(18836..19459)
                     /gene="clpP"
                     /locus_tag="KJL94_002412"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBD4410735.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19706..21004)
                     /gene="tig"
                     /locus_tag="KJL94_002413"
     CDS             complement(19706..21004)
                     /gene="tig"
                     /locus_tag="KJL94_002413"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBD4410736.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21348..21665)
                     /gene="bolA"
                     /locus_tag="KJL94_002414"
     CDS             complement(21348..21665)
                     /gene="bolA"
                     /locus_tag="KJL94_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBD4410737.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21970..22548
                     /locus_tag="KJL94_002415"
     CDS             21970..22548
                     /locus_tag="KJL94_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4410738.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22592..24067
                     /gene="ampG"
                     /locus_tag="KJL94_002416"
     CDS             22592..24067
                     /gene="ampG"
                     /locus_tag="KJL94_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBD4410739.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5717 a         6705 c         5868 g         6061 t
ORIGIN      
        1 cgaggtaatc ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat
       61 caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt
      121 acacagccac tggatcggtc aactgatcct taactgatcg gcattactcc gggcggagag
      181 ggttaacgtc gcttaatatt gcgtaggagc gggaactgcc gaagacggat tcacctgagt
      241 tggtgaagtc gaaggtacgg tagtcgttgc gccaccgctg gcctgaaccg gcacggcggt
      301 ttgctgtacc ggcaccaatg tcaggtcggc tttagttccg ccctggttga tcaccggctg
      361 aacggtatcg gtgataaata ccagtttgtc attcacggta atcgccgcac tcaacagaat
      421 acgcgcgttc ggctgaacat ctgccgggtt aaatggcaga acaaagctga atggtgactg
      481 tttaccttca gtacgcaccg ctttctgcgc cagcactttt gacggtgcat cggctaacga
      541 cgcgtcagaa agtgtcacgg tcagcacagc atcaggcggc agtgcgactt tctgacggat
      601 ccagacggta ccggagacat tcggttgctg gatagcaggt tgttgtgttg ctgaaataga
      661 cgtatttgca gccggggctg gcgtctgaat atccgcgctt ttatctgcgc aagccgccaa
      721 cgcaatcgca accgctaaac cactggccat gtgcacgagt ttcattcatt tctccttatt
      781 atcaatgcac cagcgggcta actttcctcg ccggaagagt ggttaacaaa atagtaacgt
      841 caacaagtgt ggcacacatc acgcatttct gcctgtaatt agcccgtaat tcagactatt
      901 tcacccatcg gaccagtggc aaatttgcgt tatactcaac tcactttggc ttgctgcggc
      961 agctttgtta ctggagagtt atatgagtca ggcgctaaaa aatttactga cattgttaaa
     1021 tctggaaaaa attgaggaag gactctttcg cggccagagt gaagatttag gtttacgcca
     1081 ggtgtttggc ggccaggtcg tgggtcaggc cttgtatgct gcaaaagaga ccgtcccgga
     1141 agagcggctg gtacattcgt ttcacagcta ctttcttcgc tcaggcgata gtaagaaacc
     1201 gattatttat gatgtcgaaa cgctgcgtga cggtaacagc ttcagcgccc gccgggttgc
     1261 tgctattcaa aacggcaaac cgatttttta tatgacggcc tctttccagg caccagaagc
     1321 gggtttcgaa catcaaaaaa caatgccgtc cgcgccagcg cctgatggcc tgccttcgga
     1381 aacgcaaatc gcccaatcgc tggcgcacct gctgccgcca gtgcttaaag ataaattcat
     1441 ctgcgatcgt ccgctggaag tccgtccggt ggagtttcat aacccactga aaggtcacgt
     1501 cgcagaacca catcgtcagg tgtggatccg cgcaaatggt agcgtgccgg atgacctgcg
     1561 cgttcatcag tatctgctcg gttacgcttc tgatcttaac ttcctgccgg tagctctaca
     1621 gccgcactgc atcggttttc tcgaaccggg gattcagatt gccaccattg accattccat
     1681 gtggttccat cgcccgttta atttgaatga atggctgctg tatagcgtgg agagcacctc
     1741 ggcgtccagc gcacgcggct ttgtgcgcgg tgagttttat acccaagacg gcgtgctggt
     1801 tgcctcgacc gttcaggaag gggtaatgcg taatcacaat taaaaaaata gccggaggtg
     1861 aaaaccgtcc ggctattttt tgcagtgctt gttacgcgtt ataggcattc tcgccgtggc
     1921 tgttgacgtc cagcccttct cgctcttgtt cttccggcac acgtaggcca accgtcagat
     1981 ccgccagttt gtagccaata aatgccacca caccggacca gacgatcgta atggcgatgc
     2041 tttccaactg taccagcaac tgatggccca tcgtcacacc ttcagcgaag cccacgccgc
     2101 ccagagaact ggcggcaaaa atcccggtca tgatacagcc gacaatgcca caaacgccgt
     2161 gcacaccgaa gacatcgcag ggatcatcca cccgcagcaa gcgtttgagc atggtaacgc
     2221 cccacaaacc cgccagacca gctaccacgc cgataatcaa cgcgccgcca accccaatgt
     2281 agccgcacgc tggcgtcacg ccgaccagac cggcaatcgc gccagaacac gctcccagca
     2341 gagaaggctt accacgcagc gcccattcac cgaagatcca gccaagaatt gccgccgccg
     2401 ttgcgaccac agtattcaca aatgccagtg ccgcgatttc attcgccgtg cccactgacc
     2461 cggcgttaaa gccaaaccaa ccgatataga gaatggcagt cccggtgaag accatcggca
     2521 ggttgtgcgg tttaaacgcc tctttaccga agcctacgcg tttgcctatc agatacgcgc
     2581 ccaccagacc ggcgattgct gcgttaatgt gcaccaccgt gccacccgcg aaatccagcg
     2641 caccgtgaga agccagcaaa ccaccgcccc acaccatatg cgcaatcgga atgtaagaga
     2701 gcgtcagcca taccaccacg aaaatcaaca cagctgagaa gcggattcgt tccgccagcg
     2761 ccccaactat caagcctacg gtaatgcagg caaacgatcc ctgaaacgct acgtggatat
     2821 actgataaat gctgcccatc accgccgtca gttcgatgtt ttttagcatc aaccagttaa
     2881 tgttgccgaa gaagttgttg ccctcaccaa acgccagcga ataaccgtaa accacccaga
     2941 gaatacagac cagtgcaaac gtcaccgtca cctgcgtcag catcgacagc acgtttttgc
     3001 cgcgaatcaa cccaccgtaa aacagggcaa tccccggaat agtcataaac agcaccagcg
     3061 cagtacaaat catcataaac gcattgtcgg ctttatcggc caccgcaggt gcagccatta
     3121 ccagtcccgg cagcatcgcc agtgaagcaa gcccagtttt tatcgtcgct atcttcattt
     3181 ttcgttcctg ttgctgtgtg ccagagatta cagcgccgct tcgtcggctt cgccggtacg
     3241 aatacgaatg acgcgttgca attcagcgac gaagattttg ccgtcgccaa tttttccggt
     3301 gtaagccgcc ttactgacga tatcgatcac ttcatcgagt tggtcatcag caatcgccac
     3361 atcaattttt acttttggca ggaagttgac gctgtattcc gccccccggt acagctcggc
     3421 atgccctttc tgacgcccga aacctttcac ttcggtgacg gtcaggccct gaataccaat
     3481 ggaagataac gcttcacgaa cgtcttccag cttgaatggt ttgattatca cggtcaccag
     3541 cttcatagat cccctccggt cagaattcgg taatggttct gctacacgaa gaaggtattg
     3601 caagcggtgt gccagaaatg aaaaacgaga aagagagcag gaagttaaga agattgaaaa
     3661 tgaaaacgca ctatgacagt gcacagtgcg ttacattttt gcaccaacga tgaaatattg
     3721 cgctattcag gcgctcaatg attcctcttc acgcacgctg gctgccagct cttcgcccgc
     3781 aagttgcagt tgatacatct gccagtagcg tccctgggcc gccagcagtt gctggtgagt
     3841 gccctgctcc acggcttgcc cacgatgaag caccagaatg gtgtcggcat caataatagt
     3901 tgataagcgg tgagcaatca ccaccagcgt ggtatgttca cgcaccgccg ccagagcatg
     3961 ttgaatcgcc tgttcagtac cggagtcaat gctggcggtt gcctcatcaa ggatcaggat
     4021 ttgcggcgtc tccaccagca cgcgcgccag tgccagcagt tgcttttgcc cgactgagag
     4081 attatttccc tgctcgccca gcggcgtata aatacaatca ctcatgctgc gcgccagctc
     4141 cgccagttgc acggtttcca gcgcctgcca gacgcgttct tcggagatat cccgccccag
     4201 cgtcacatta gcgaggaagg tatccgccag caccaccgga tcttgctgca ccattgccac
     4261 gccctggcgc agcgcgctat gacttagcga acttaatgga cgaccatcaa ggtgaatctc
     4321 accttccgtt agcgggtaat agcccatcaa taaactggcg agggtacttt taccactgcc
     4381 ggtatgcccg accagcgcca caaagttacg cgaaggcaca gagagattaa tgttctttag
     4441 caccagattg tcatcgcgat aagcaaatga cacgttatcg acttcgatgg tgccactctg
     4501 taacgggtaa tcatcattgc catattgctg gcgcggtccg tccatcagtt caaacacgcg
     4561 ctcaccagca acaacagcct gttgcagcat cgcctgttgc gtggtcagtt cgattaaggg
     4621 ttcgttaagt cgcccaagat agctgataaa cgcatacaac acgcccactt caatggtgcc
     4681 gctggcggag aagccaaaca gcatcaataa gccacaaaga atgagcgatg aaaacagact
     4741 cagcagcgga cgcagcagaa aaccgtcgag gcgcagggtt tgcatcctag ccatatagtg
     4801 tgaacgactg gcctccccca tacgttcgcc aaatcgcgcc tgctgacgaa actgctggat
     4861 aacgctcatg ccattgatga tttcgttaaa gccgtcgttg atatccgcca gataggcgcg
     4921 cacacggcgg acaatcggcg tgctgtaacg ctggtatatc accattacca ccagcaccac
     4981 cgggaaaatc attatcgcca ccagcgccat tcgccagtcg aggctgaaca tcgccaccag
     5041 catcgcgccc accagtgcgg cactgcgcag gacagttgcc actacggtaa cgtagagatc
     5101 gcggatcact tcagtgtcat tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc
     5161 aaactcgctt aatggctggc gtaacgcagc atccatcacg tcggtacgca actgttgcac
     5221 tgcgcctact gccgcccgat taaacagcag cgactgcgcg taatgtagcc cggcggcaaa
     5281 cagttgtagc ccaacatacg ccgcagccag ccctgcaacc actttcaacg gcaggttatt
     5341 tttcgctacc atattgtcga taaaatagct gataagcagc ggcccactga cttctgccgc
     5401 cgccgcaacc cacatcatca ggaccgcaat ccccagcggt ttacgccacg gcgaaccata
     5461 cgctaacagg cgcttgagag tcggccacag ttggctaaaa ctacgcatcg acggcctcct
     5521 cgcgaatttc cggaacgtcg tcgagcgccg cctccagttg ttgatagcga tacatatcgc
     5581 gataccaacc gctttgttgc gccagctcat catgattgcc acgctgggcg atatgtccgt
     5641 gctgcatcac aataatttca ctggcttccg tcagtgccga aaggcgatgg gcactgatga
     5701 ttaccgttct tccctgtccc cactgacgca ggttatgcag gatctggtgc tctgtgcgtc
     5761 cgtccaccgc cgaaagcgca tcatcaagga tgaggatttc cgcgttgact aataacgctc
     5821 gagcaatgga gatacgctgt ttttgcccac cggaaagcat cacgccgcgc tcgcccactt
     5881 ctgtatcgta accttgcggt agacgcaaaa tatcgtcatg tacgctggct aaccgcgcga
     5941 catgctcaat ctcttgctgg gtggcattcg ggcaacccag cgcgatgtta ttcgccacgg
     6001 tgtcagaaaa aaggaatggc gtctggctga ccaccgccag gcggctacgc cagctatcga
     6061 gttgtaactt cgtcagagga atatcatgaa agcgaatatc cccctcgctg acgtcgaaat
     6121 gacgctgaat gagcgacaac agggtacttt tgccggaacc agtcggcccg caaataccca
     6181 gcatctgacc gggtttcagg gcgaaattga cgttttccag cgcaggatgg tcagtctgcg
     6241 gatacgtgaa ctggtgaata tttacatcca gttcgccacg cccttccggc acgggttcgc
     6301 taccatcgtt caccaccggc gcttccgcca gcatcgcgcg aatacggctg tatgcagcac
     6361 taccacgttc cacaatgtta aacatccatg ccagcgccag cattggccaa atcatcagac
     6421 ctaaatacat cataaaactg gtgagctggc ccagcgttaa actgccctgc accaccatcc
     6481 aactaccgcc gccaatcgcc agcaagttcg ccataccaat cgcgatatag atggtcgggt
     6541 cgaagcgagc atcaatacgc gccacccgca tgtttttttt gccggtatct tcggcatccg
     6601 cggcaaataa cgccgactgg cgatcttcca gaccaaaggc tttgatcatg cggatactgg
     6661 tgaggctttc ctgggtgcgg tcattaagac tggaaaacgc cgcctgtgcc agcttaaagc
     6721 gttcatgcaa ggcatcgccg ttgcgcttaa tcatgatcgc catcactggc atcggtaaca
     6781 gggcaaataa ggtcaactgc cagctaattt gcgtagacat cataatcaac acagcgcagc
     6841 ccattaccag tgaatccacc agcgtcagca ccccttctcc ggcggcaaac acgacgcgat
     6901 cgacgtcatt ggtcgcacga gccatgagat cacccgtgcg atgacgcagg taaaactccg
     6961 ggtgctgccg gcttagctga cggtaataat cttcacgcag ttcaacagcc agttggtaag
     7021 acgcgccaaa cagcaatacg cgccagacat aacgcaggag ataaaccaca acggcaatca
     7081 gcaccatggt ggcgatccac atcaggctca gcccggtagt aaagtgttgt tctgtcacgc
     7141 catcgacaac aataccaacc acttttggcg gaaccagttg cagcatcgcg ataatgacaa
     7201 gcaaggcgac agccccaaga tagcgacgcc attcccgacg gaaataccag cttaattgag
     7261 caaataatcg cacgcgttgt gtcctgacct gattctggat atttattcga tgggtaaaga
     7321 agtggtgtat ttaatctgtt ccatcgcgaa gctggaagtg acgtccgaca gccccggcac
     7381 gctgtttacc agacgcttat aaaactcgtc gtagcgtttc atgtcggcaa cctggacgcg
     7441 catcagataa tcgtattcac cagccatgcg ccagaccccc agcacttctg gcatttcggt
     7501 aaccaccgtg acaaagcggc aataccattc gctgctgtga tgttgcgttt ttatcagcac
     7561 aaaggcggtc aggccgaggc ctattttttc cggatccagc agggcgactt tgccgataag
     7621 gataccgtcg tcctccagcc gtttcaggcg cttccagcag ggggtggttg tcagattaac
     7681 ggcttcagcc agtgcctgca aagagagggt gcaatcctgc tgcagtaagg ccagcagctt
     7741 acggtcaatt ttatctaaca tagcccttcc acggagaatt tttttctctt ttaattctat
     7801 tttaaaggtg aaatcgcgac aatttattct gtgcggttta aacaccacgg cacaaaatga
     7861 caaattcaac gcaaaaaaaa accgggcaat tgcccggtgc tggaagggat ctcgttattc
     7921 gggggaataa ggtagatgtg gatagtccag ccagtgcgtc aaatagtgag agacagcctg
     7981 atttcggcaa tgtccaatca ccggtaaatg cgggagctcc gcgcgcagtt gcggcatcgc
     8041 attgcccata ataaacccgc taccgacgct gcctaacatt tcgcgatcgt tcatcgcatc
     8101 accaaaggcc atgcaatcgc gcaacgataa acctaaatgt tgggtccgca ccgtcaatgc
     8161 agcgccttta ttgcagccca ccggcagcac ttcgaggcaa tccgtggcgg aaaaacacaa
     8221 atgtgcacgc tcgcctaatg cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg
     8281 atcgccacag aagcagatct tggtgacgct gccgagtggc atttttttga catcgattat
     8341 ctgataacga aaaccgctat agacaaatac ctgcaacaac gcagggctct ctttcccggt
     8401 aaaccaaccg tcgtcattga agatatgcat gctggctcgg gtatcccatt gctgatacag
     8461 caccagctcc gcgacatccg caggtaaatc atcacgatgt aaaagttcac cttccagaga
     8521 atgcacgcgc gttccgttgc cggtaatcaa atatgcatcc agcgatagcg ccccgagaat
     8581 atgctgcatc tccagcgcat gacgccccgt ggcaaaagtg agggtaatgt cgcattcacg
     8641 cagtcgcgcc aaagtagaga gggttttctc acctaaatga tggtcaggca tcaataaagt
     8701 gccatccata tcaaatgcta ccagacgagc catttctttc tccactctgt gacacggtta
     8761 atggtgattg agtatcacct gatatatacg gaagtaatag tgaatagtta ataatattgt
     8821 tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg tggcaacctt
     8881 cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag cgaacgccat
     8941 gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca ggcgcagtca
     9001 ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct acgcaatgcg
     9061 atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct ggcgcaactg
     9121 gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg gcaaaacgat
     9181 acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc aggctttttg
     9241 cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac ccgcttcgat
     9301 aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc tactgacagg
     9361 ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga tgcagtaaaa
     9421 gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact ggatcaatta
     9481 tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt ctttttacat
     9541 cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct ggcgcatccg
     9601 caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc ggagctggtg
     9661 cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc ggttgagtac
     9721 tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca tcccgtgcaa
     9781 atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag tggcatcagt
     9841 ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg gcaggcgcga
     9901 aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt cggagaaaac
     9961 ctgatcaccg ccagtcatgc attactgcta ggctggacta ttccgcactg gcaagtaccg
    10021 gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat agaacttcat
    10081 accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact cacaattatt
    10141 tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga cctcatgatg
    10201 ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct acgttgcgat
    10261 ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc gacgctggac
    10321 gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc ggtttttagc
    10381 cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg cattagtgcc
    10441 cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt taccgaagcc
    10501 tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc gttaccataa
    10561 cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt tcagtggagg
    10621 ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag tccattgcgt
    10681 gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg aactggcgct
    10741 gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg agctggcggt
    10801 cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag ccgatggcat
    10861 cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg caatatatgc
    10921 gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt tctccggcta
    10981 cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt tgggcatggc
    11041 gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa cctgggcgct
    11101 ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct gctataacgg
    11161 cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca acggtttgaa
    11221 tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa ccgggttgag
    11281 gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa aacgtttact
    11341 taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct ttctgcgttt
    11401 taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct tccggctcca
    11461 gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac tgactggtaa
    11521 tagttaacag gtcgcttaat accgctgggc gacgatagtt aatattgata ttgaccacga
    11581 cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc accccatccc
    11641 agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg taaacgtcga
    11701 gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac gttgttgtta
    11761 taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc
    11821 aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg cccatccccg
    11881 gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa
    11941 tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg
    12001 cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt actgctgctt
    12061 tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc gccacagaag
    12121 ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagggta atgagcagtg
    12181 ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac
    12241 gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac
    12301 cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat tattgctgtt
    12361 ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa
    12421 agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgatcttccg
    12481 gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg
    12541 tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc
    12601 taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc agaccggcag
    12661 cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca
    12721 ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat
    12781 ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg aatgcgcggt
    12841 cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca cctaccagac
    12901 cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc aggttatctt
    12961 tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca ccggccagag
    13021 actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag tacgcatcga
    13081 gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct aacgatttca
    13141 ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga
    13201 caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg gggatagtgg
    13261 catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg gcagattttt
    13321 ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt
    13381 catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat
    13441 cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg
    13501 cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt
    13561 tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt
    13621 taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc gccagctcgt
    13681 cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag
    13741 tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga
    13801 taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa
    13861 tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg tactgatcca
    13921 acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt
    13981 cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga ttacgctcgc
    14041 tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact tttgcggcgt
    14101 agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa
    14161 tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca
    14221 tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc
    14281 aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac agaaagtcac
    14341 cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac
    14401 tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac
    14461 tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc
    14521 acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag
    14581 agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat
    14641 atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt
    14701 tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc
    14761 agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc taacttagcg
    14821 agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt
    14881 ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg
    14941 ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg gcaattacgt
    15001 tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact gttttaatcc
    15061 cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt acctgaccac
    15121 gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag
    15181 aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt
    15241 ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc cccagttttt
    15301 ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc acttcgccga
    15361 gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca atggtcagca
    15421 agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg ttttcgttat
    15481 cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta tcgccgttaa
    15541 tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg cggcacagtt
    15601 tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc
    15661 cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct
    15721 gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga
    15781 ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg
    15841 cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta
    15901 cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct cgcatgtcag
    15961 aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc actttcgcca
    16021 ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt
    16081 ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg gtggctttgg
    16141 caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg cagaggatcg
    16201 gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg cgatctttca
    16261 cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt
    16321 tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa ccacgcacta
    16381 cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt
    16441 tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg gcttcgtttt
    16501 cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca
    16561 gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct
    16621 caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt
    16681 taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag
    16741 caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc agcacttctg
    16801 gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca
    16861 gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa
    16921 agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca
    16981 ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg
    17041 tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa
    17101 tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa
    17161 ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt
    17221 caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt
    17281 aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga
    17341 tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc
    17401 cctttttggt taactaattg tatgagaatg gttaattatt caccagatgc ctgttgcgct
    17461 tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga ctcgtcgata
    17521 accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag cagtgcggct
    17581 tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata
    17641 gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga
    17701 tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc
    17761 agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat aagaccaaac
    17821 ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac
    17881 ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg gtgggaaatc
    17941 actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc
    18001 tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc agctaccgta
    18061 ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc tcgggtaatg
    18121 gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc
    18181 tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat gttttcaacg
    18241 tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg
    18301 tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt cggaccgatc
    18361 agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt gcgcagacgt
    18421 ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg
    18481 taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc
    18541 ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg
    18601 cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa
    18661 aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac
    18721 ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg
    18781 cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc tggcatcaat
    18841 tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga
    18901 agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca
    18961 taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg
    19021 tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc
    19081 aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag gccgcctggc
    19141 ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc
    19201 cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt tctgggtttt
    19261 ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc
    19321 cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac
    19381 cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc
    19441 gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc tgctgtaccg
    19501 attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg gcatcaccat
    19561 atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt ttcgcacggc
    19621 tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg cctttgtgcg
    19681 aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg ttgaaagtgg
    19741 tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt tcttccagag
    19801 caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg ataacttctt
    19861 tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc tcttcgtcag
    19921 ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact acgcggcgtt
    19981 tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg ttgccaccga
    20041 aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc gcagccggta
    20101 cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg cggttacgga
    20161 tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga ccttctacgg
    20221 aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc agttcacgct
    20281 cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg ttttctgcgt
    20341 ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg tggcctttga
    20401 taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg aaatcagacg
    20461 ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg gttacgcggt
    20521 cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta cgcagagtat
    20581 ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca acttcgatcg
    20641 cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag taagtgaagt
    20701 cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga ttgatttttt
    20761 ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg tcctggcgta
    20821 cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg cggaagccgt
    20881 caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc tcgatgctgt
    20941 cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca actgaaactt
    21001 gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc aagcacaggt
    21061 tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt taccagaatc
    21121 atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta tgagtcgaga
    21181 acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt cgtctgaatg
    21241 ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt ttttcataaa
    21301 atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta cgcgatgctt
    21361 cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc ccactcctta
    21421 atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc cgctaaagta
    21481 ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc gctgaccagc
    21541 acaactttaa aatggctttc agagccggct gggacattgt gacgatagct ttcatccact
    21601 acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg ctcacgtatc
    21661 atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa atactagccg
    21721 cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct ggtttttccg
    21781 tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat cagcgtttct
    21841 tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc cacttcccct
    21901 cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag agtctctgag
    21961 agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat gcttgcagga
    22021 tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc acagcaggat
    22081 ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac cgatcaggcg
    22141 ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg cgctctgcgt
    22201 ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat ggttggtccg
    22261 aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt gtcccagggc
    22321 aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac cgcgcagaat
    22381 ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc gttccaggcc
    22441 tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat cgctgatatg
    22501 tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt tctgccctct
    22561 ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac gtatttttca
    22621 acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac cgctcgccct
    22681 gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca aaaccattgg
    22741 tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac cgctgatgga
    22801 ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca cgcaaatcct
    22861 gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac tccgctggat
    22921 ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg tcttcgatgc
    22981 gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa tcagcgtgct
    23041 gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg cagataaatg
    23101 gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct gtattatcgc
    23161 gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc tggaacaagc
    23221 ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta ttttgcttct
    23281 tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca cgtttttgat
    23341 tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc ttggcttact
    23401 ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt cgctgttccg
    23461 ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact ggctgctgtc
    23521 gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa acctctgtgg
    23581 cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata agtcattttc
    23641 cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt atgtcggccc
    23701 cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat tctccgtcgc
    23761 cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg aatatacacg
    23821 agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct ttgccatgtg
    23881 gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga cgatggacgc
    23941 gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg ttttagtcgc
    24001 cctttctggc gtcatgcttg gtggtttgct ggattatctg gcgctacgaa aaacgcatct
    24061 gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta ttacggctaa
    24121 ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg tttggaaatt
    24181 attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag tctttatttt
    24241 ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt ttatttcgca
    24301 ttggttatac caattgcccg ccaagactcc ttctgcactc cccttttgtt a
//
LOCUS       DAEBXD010000057        24318 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEBXD010000057 DAEBXD010000000
VERSION     DAEBXD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24318
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.057"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            138..1220
                     /locus_tag="KJL94_002417"
     CDS             138..1220
                     /locus_tag="KJL94_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBD4410740.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1709..2507)
                     /locus_tag="KJL94_002418"
                     /pseudo
     CDS             complement(1709..2507)
                     /locus_tag="KJL94_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2517..3068)
                     /gene="ybcL"
                     /locus_tag="KJL94_002419"
     CDS             complement(2517..3068)
                     /gene="ybcL"
                     /locus_tag="KJL94_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBD4410741.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3237..3569)
                     /gene="emrE"
                     /locus_tag="KJL94_002420"
     CDS             complement(3237..3569)
                     /gene="emrE"
                     /locus_tag="KJL94_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBD4410742.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3914..4228)
                     /gene="fliE"
                     /locus_tag="KJL94_002421"
     CDS             complement(3914..4228)
                     /gene="fliE"
                     /locus_tag="KJL94_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBD4410743.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4442..6107
                     /gene="fliF"
                     /locus_tag="KJL94_002422"
                     /pseudo
     CDS             4442..6107
                     /gene="fliF"
                     /locus_tag="KJL94_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6100..7095
                     /gene="fliG"
                     /locus_tag="KJL94_002423"
     CDS             6100..7095
                     /gene="fliG"
                     /locus_tag="KJL94_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBD4410744.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7088..7774
                     /gene="fliH"
                     /locus_tag="KJL94_002424"
     CDS             7088..7774
                     /gene="fliH"
                     /locus_tag="KJL94_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBD4410745.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7774..9147
                     /gene="fliI"
                     /locus_tag="KJL94_002425"
     CDS             7774..9147
                     /gene="fliI"
                     /locus_tag="KJL94_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBD4410746.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWKASLQGLE
                     RIFPTVS"
     gene            9169..9510
                     /gene="fliJ"
                     /locus_tag="KJL94_002426"
                     /pseudo
     CDS             9169..9510
                     /gene="fliJ"
                     /locus_tag="KJL94_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            9507..10634
                     /gene="fliK"
                     /locus_tag="KJL94_002427"
     CDS             9507..10634
                     /gene="fliK"
                     /locus_tag="KJL94_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBD4410747.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10739..11203
                     /gene="fliL"
                     /locus_tag="KJL94_002428"
     CDS             10739..11203
                     /gene="fliL"
                     /locus_tag="KJL94_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBD4410748.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11208..12212
                     /gene="fliM"
                     /locus_tag="KJL94_002429"
     CDS             11208..12212
                     /gene="fliM"
                     /locus_tag="KJL94_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBD4410749.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12209..12622
                     /gene="fliN"
                     /locus_tag="KJL94_002430"
     CDS             12209..12622
                     /gene="fliN"
                     /locus_tag="KJL94_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBD4410750.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12625..12990
                     /gene="fliO"
                     /locus_tag="KJL94_002431"
     CDS             12625..12990
                     /gene="fliO"
                     /locus_tag="KJL94_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBD4410751.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            12990..13727
                     /gene="fliP"
                     /locus_tag="KJL94_002432"
     CDS             12990..13727
                     /gene="fliP"
                     /locus_tag="KJL94_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBD4410752.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13737..14006
                     /gene="fliQ"
                     /locus_tag="KJL94_002433"
     CDS             13737..14006
                     /gene="fliQ"
                     /locus_tag="KJL94_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBD4410753.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14015..14800
                     /gene="fliR"
                     /locus_tag="KJL94_002434"
     CDS             14015..14800
                     /gene="fliR"
                     /locus_tag="KJL94_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBD4410754.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15085..15708
                     /gene="rcsA"
                     /locus_tag="KJL94_002435"
     CDS             15085..15708
                     /gene="rcsA"
                     /locus_tag="KJL94_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBD4410755.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15752..15940)
                     /gene="dsrB"
                     /locus_tag="KJL94_002436"
     CDS             complement(15752..15940)
                     /gene="dsrB"
                     /locus_tag="KJL94_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBD4410756.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16103..16330
                     /gene="yodD"
                     /locus_tag="KJL94_002437"
     CDS             16103..16330
                     /gene="yodD"
                     /locus_tag="KJL94_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBD4410757.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16628..17443
                     /locus_tag="KJL94_002438"
                     /pseudo
     CDS             16628..17443
                     /locus_tag="KJL94_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17440..19134)
                     /gene="yedQ"
                     /locus_tag="KJL94_002439"
                     /pseudo
     CDS             complement(17440..19134)
                     /gene="yedQ"
                     /locus_tag="KJL94_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19305..19487)
                     /locus_tag="KJL94_002440"
     CDS             complement(19305..19487)
                     /locus_tag="KJL94_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBD4410758.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19566..20483)
                     /locus_tag="KJL94_002441"
     CDS             complement(19566..20483)
                     /locus_tag="KJL94_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBD4410759.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20656..21576
                     /gene="yedA"
                     /locus_tag="KJL94_002442"
     CDS             20656..21576
                     /gene="yedA"
                     /locus_tag="KJL94_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBD4410760.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21565..22035)
                     /gene="vsr"
                     /locus_tag="KJL94_002443"
     CDS             complement(21565..22035)
                     /gene="vsr"
                     /locus_tag="KJL94_002443"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBD4410761.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22016..23434)
                     /gene="dcm"
                     /locus_tag="KJL94_002444"
     CDS             complement(22016..23434)
                     /gene="dcm"
                     /locus_tag="KJL94_002444"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBD4410762.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23501..24041)
                     /locus_tag="KJL94_002445"
                     /pseudo
     CDS             complement(23501..24041)
                     /locus_tag="KJL94_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24081..>24318)
                     /locus_tag="KJL94_002446"
     CDS             complement(24081..>24318)
                     /locus_tag="KJL94_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410763.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6094 a         6263 c         6042 g         5919 t
ORIGIN      
        1 agataaaaat agggatctac ttcacaattt aaatgagaaa ctaaaactta catcttgaaa
       61 taatctcatt gattggatga atatttgtag tgcagtgaca tcatttttaa ataatagttc
      121 aaataaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag
      181 tactgatggc gatgtctgct caggcagcag agatttacaa taaagacagt aacaagctgg
      241 atctgtacgg aaaagttaat gctaagcact acttctcttc taacgatgca gatgatggcg
      301 acacaactta tgttcgtctg ggcttcaaag gcgaaactca aatcaacgat caactgactg
      361 gtttcggtca gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa ggttcttcca
      421 aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc tccatcgatt
      481 atggccgtaa ctacggtgta gcatacgata tcggtgcatg gaccgacgtt ctgccagaat
      541 tcggtggcga tacctggacc caaacagatg tgttcatgac tggtcgcacc acaggtgttg
      601 caacctatcg taacaacgac ttctttggtc tggttgatgg tctgaacttt gctgctcagt
      661 atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa cggtgatggt ttcggtttct
      721 ccactactta tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa tctgaccgca
      781 ctaatgatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa aatgctgaag
      841 tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctacc acctattctg
      901 aaacccagaa catgactgtt tttggtaata accatattgc caacaaagca caaaacttcg
      961 aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct tacctgcaat
     1021 ctaaaggaaa agacttgggt gcgtggggtg atcaggacct gattgaatat attgatgtag
     1081 gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgattacaaa atcaacctga
     1141 ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc gttgctgtag
     1201 gattggttta ccagttctaa tctgattact caaagatatg ttgtgggagg ctttgcctcc
     1261 ccaacatata agtggcgctc tcaagccact ttccttagaa gcactatctt gcttctgact
     1321 atataaatta ccctttattt tctgggacgt ttcaacgccc catttttaat aatactcagt
     1381 gaacaattaa cgtatcaatt agatttaata acaacgatat ccgtacctaa cctgatatct
     1441 aatgtcataa gcatcccttt aattatcccc tcaacctctt gtaaatttcc cactcttgag
     1501 ctccacatag cccaaacgcc ggatgcaaaa aaaatcacac gtgcatctct gaaattgtga
     1561 tggaggaagt gagtatcaat tatatgtgct tataaacgtt cccgattggc acagaagtca
     1621 ccggattgtt caggctccaa taggttgagt atgtcgtatt tattttccaa aataaaaatc
     1681 gatgctgttg ctccttaata aaatactttt actctctgga cgcttttctt agctctcttg
     1741 gtgttatatt aaaacgatta tggaaccttt cagtaaaacg agaaggacac ttgtaaccat
     1801 tttccctggc aatctcgctg atgggtttta ctgtcgtttg tatagcagga caacgcatta
     1861 tttaacctca cgtcgtccaa tatactttga aaacttattc cctcgcttgc cagacggcga
     1921 tgcaatgtgg aaacagaaat gtagagatat ctcgctacct tgtttgctgt ccattttgtt
     1981 ctaggttcgg atagcattag gttataacaa cgacttatta atgattgttt actatctgat
     2041 aaaagtaaac aattaacatg atgtgctcct aatgaaagta gaacgcccat tgctaagtgt
     2101 tcctgaattt gcgttgagaa gcctcgggaa atagatgttt ttagttgctc ccaacaatat
     2161 attaactcgg ggttctgagg taagcagaaa cctgtcgtgt tacgaatttg atcagttatc
     2221 gcataaaatt tttgaaactt ttcaattaga tcaatgggat aataaagcat ttctgcaaga
     2281 taaagacctg cttctggata attcacaata taaaattcat atccacaggg aaataatatt
     2341 atctgattat tatcaacagt tagactatgc gtctcccaat tgataacttt ttttccctga
     2401 cggatacgac ataaagctgg cataagaggc ttaaccctgt gaatctcatg atgtttatgc
     2461 atccgtattt cttcaatctt taggttagac ttatctcttg ccagcatact cttaccctat
     2521 tttatctcat aaactggtgt tatctcagcg gttgcaattt tattggcatt aagcatataa
     2581 ccaactaacg ctccgctgga attagaatcc acaggaatct tatcagtttt tagagcccat
     2641 actttaaact ggtaatgatg gggtttatct cctttgggag gacatgctcc accaaatcct
     2701 gcataaccaa aatcatttcg gccttgaaca gcaccagtcg gaagttttgt accatcacgt
     2761 cttcctgcat cagtgggcaa atatgttaca gttgctggaa tattagcaac agtccagtgc
     2821 caccatccac tgcctgtagg agcatctgga tcatacacag ttacggcaaa gcttttggtg
     2881 ccttcaggag caccagacca ggttaatgag ggcgatgtat taccaccttc acacccaaat
     2941 ccagaaaata catgagacgt tgtaagttgc tctcctgttt ttatttcatt actagtgacc
     3001 tgaaatgctg cggcctgcgc agaaaatgtt atgaatgcca atacacttga aacgataagt
     3061 tttttcataa aaacctcatt gttgtgacct atcgttattt tatttgatgt gccttgatct
     3121 cattatgaat aaaggcgcaa tgatcgcgca aatgcaatca taattataaa ggtgccccga
     3181 acattagcca taatatgcac aaatcagatt cctgttatct caaggagaat tattttttaa
     3241 tgcggtgcac ttcgtgacaa caagttaatg actaacacac cggcacaaat caacatcatg
     3301 cctatgatag ctggcaggtc cagtcgttga ccaaaaaatc cccaggaaag caaactaatc
     3361 agaacgatac cgactcctga ccagatagca taagcaatcc ctgtaggtat ataagccagc
     3421 gtctgagcta ataaccagaa tgatgcacaa tagcaaataa ttgtgccaac agagggccat
     3481 aaccgtgtaa acccctctga aaacttcatt aaggttgtac caatgacctc tgcaagtatt
     3541 gcgccaccaa gataaatata agggttcata gcatattctt tcctgttcat actggagaga
     3601 attgtactac agtttgaaca caactcaccg ttttcatcct ggtgtatgtg tatccaaggg
     3661 gctctggcgt ggcaagagat aaaggcagga acaataatga tcagaaggaa ccccaggcta
     3721 acgaatgagt tcactcttcc tgggagcaaa gcgaagacaa aacatgttat cgcgtttacg
     3781 gatcgtggat ggcagaaaac aaccaggatc aggtatccat tttaaaccag aaattgagtg
     3841 agtttgcccc atccatgccc cacgctgtgg gatcggatat aattaaacaa gcatagtcaa
     3901 caggttacac aacctacacc tgcatggaca tcacttcctg atacgccgcc accagcttat
     3961 tacgcacctg aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat
     4021 cgtttagcgc cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg cgggcgactg
     4081 tttgtgtatc gcttatgcga tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg
     4141 gttgcggcag tgattcctgc gcacacgcac tcatcgccgt agcctgtaac tggctgataa
     4201 ccccttcaat cccctgtatc gctgacattt tcgtctcccg gataatttct ggtaacaaag
     4261 cctaccagta agtcaataag acaaaggcgc taaatagcaa caaaaaaact ggtttattgg
     4321 cggatagaaa aaacgaaagc acaaataatg ggagcgtcaa tttttcgagt ttgctgaccc
     4381 gagagtgagt cttgttccac tttgccaata acgccgtcca taatcagcca cgaggtgcgc
     4441 gatgaatgcg actgcagccc agacaaaatc tcttgagtgg cttaatcgcc tgcgtgcgaa
     4501 tccgaaaatt ccattgattg ttaccggttc cgcggcagtg gcggtcatgg tcgcactgat
     4561 cctgtgggcg aaagcccccg actaccgcac attattcagc aatctttccg atcaggatgg
     4621 tggcgcaatt gtcagccaac tgacgcaaat gaatattcct taccgcttca gcgaagccag
     4681 cggcgctatt gaggttccgg cagataaagt tcacgaactg cgcctgcgcc tggcgcaaca
     4741 aggtttgccc aaaggcggcg cggtcggttt cgaactgctc gatcaggaaa agtttggtat
     4801 cagccagttc agcgaacagg tgaattatca gcgggcgctg gaaggcgagc tttctcgtac
     4861 catcgaaact atcggcccgg taaaaggggc gcgcgtacat ctggcaatgc cgaaaccgtc
     4921 tttattcgtc cgtgaacaaa aatccccttc tgcatcggtg acggtaaatc tgttacccgg
     4981 ccgcgcactc gatgaagggc aaattagcgc tattgtacat ctggtttcca gcgcggttgc
     5041 tggtctgccg cctggaaacg tcacgctggt ggatcagggc gggcatctgt taacccagtc
     5101 caataccagc gggcgcgatc ttaatgacgc tcagttgaaa tatgccagcg atgtcgaagg
     5161 ccgtattcag cagcgtattg aagcgatcct gtcacctatt gttggtaacg gtaatattca
     5221 cgcctaggtc acggcgcaac tggacttcgc cagtaaagaa caaacggaag aacagtatcg
     5281 ccctaacggt gatgaatctc atgcggcgct tcgttcacgc cagcttaatg agagcgagca
     5341 aagcggttcc ggttatccgg gcggcgtacc gggggcgttg tcgaatcaac cggcacctgc
     5401 gaataacgcg ccaatcagca cgcctccgac aaatcaaaat aaccgccagc agcaggcgag
     5461 caccaccagc aatagtgggc cgcgtagcac acagcggaat gaaaccagta actacgaagt
     5521 cgatcgcacc attcgtcata ccaaaatgaa cgtgggcgat gtgcaacgtc tgtcagtcgc
     5581 ggtagtggtg aattacaaaa ccttgccaga cggtaaacca ttgcctctca gcaacgaaca
     5641 gatgaagcaa attgaagctc tgacccgcga ggcgatgggc ttttctgaaa aacgcggtga
     5701 ttcgctcaat gttgttaact cgccgttcaa tagcagtgac gaaagcggcg gagcactgcc
     5761 gttttggcaa cagcaagtgt ttatcgatca gttgctggct gccggtcgct ggttgctggt
     5821 tgctggtact gctggtggcg tggttgctgt ggcggaaagc ggtacgcccg cagctaacac
     5881 gtcgcgccga ggcggtgaag actgtgcagc aacaggcgca ggcccacgag gaagtggaag
     5941 atgcggtgga agtccgtctg agcaaagacg aacaactcca acaacggcgc gctaaccaac
     6001 gactgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac gatccgcgcg
     6061 tggtggcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct gacaggcacc
     6121 gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag
     6181 cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc
     6241 tccaacaagc agctaaccga tgtgctggcg gagtttgagc aagaagctga acagtttgct
     6301 gcactgaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc tctgggtgaa
     6361 gaacgtgccg ccagcctgct ggaagatatt ctcgaaaccc gcgataccgc cagcggtatt
     6421 gaaacgctca actttatgga gccgcagagc gccgccgatc tgattcgcga tgagcatccg
     6481 caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggccgccga tattctggcg
     6541 ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg
     6601 cagccagccg cgctggccga actgaccgaa gtactgaatg gcttgctcga cggtcagaat
     6661 ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg aaattatcaa cctgatgaaa
     6721 acgcagcagg aagaagccgt tattaccgcc gtgcgtgaat tcgacggcga gctggcgcag
     6781 aaaatcatcg acgagatgtt cctgttcgag aatctggtgg atgtcgatga tcgcagcatt
     6841 cagcgtctgt tgcaggaagt ggattccgaa tcgctgctga tcgcgctgaa aggggccgag
     6901 cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc
     6961 gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg tcgaaaacga acagaaagcg
     7021 attctgctga ttgtgcgccg tcttgccgaa actggcgaga tggtgattgg cagcggcgag
     7081 gatacctatg tctgataatc tgccgtggaa aacctggacg ccggacgatc tcgcgccacc
     7141 accggcagag tttgtgccaa tggtcgagtc ggaagaaacc attattgaag aggccgaacc
     7201 cagccttgag cagcaactgg cgcaactgca aatgcaggcc cacgagcagg gttatcaggc
     7261 ggggattgcc gaaggtcgcc agcaaggtca cgaacagggc tatcaggaag gactggccca
     7321 ggggctggaa caaggtctgg cagaggcgaa ggctcaacaa gcgccaattc atgcccggat
     7381 gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg cttgatagtg tgatagcttc
     7441 gcgcctgatg cagatggcgc tggaggcagc acgtcaggtg atcggtcaga caccaacggt
     7501 ggataactcg gcactgatca aacagatcca acagttgttg cagcaagaac cgttattcag
     7561 cgggaaacca cagctgcgcg tacacccgga tgatctgcaa cgtgtggatg atatgctcgg
     7621 cgcaacttta agtttgcatg gctggcgctt gcggggcgat cccaccctcc accctggcgg
     7681 atgtaaagtc tccgccgatg aaggcgatct cgacgccagt gtcgccactc gctggcaaga
     7741 actctgccgt ctggcagcac caggagtggt gtaatgacca cgcgcctgac tcgctggcta
     7801 accacgctgg ataactttga agccaaaatg gcgcagttgc ctgcggtacg tcgctacggg
     7861 cgattaaccc gcgctaccgg gctggtgctg gaagccaccg gattacaatt gccgctcggc
     7921 gcaacctgtg tcattgagcg ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc
     7981 gttggcttta acggtcaacg gctgttttta atgccgctgg aggaagtgga aggtgtcctg
     8041 cccggcgcgc gtgtatatgc caaaaacatt tcggcagaag ggctgcaaag cggcaagcag
     8101 ttgccgctcg gtccggcgtt attaggtcgt gttctggacg gtagcggtaa accgctcgat
     8161 ggcctgccct tccccgatac gacggaaacc ggtgcgctga ttaccccgcc atttaacccg
     8221 ttgcaacgta caccgattga acatgtgctg gacaccggcg tgcgcccaat caatgccctg
     8281 cttaccgtcg gacgcgggca gcgtatgggg ctgtttgccg ggtcgggcgt tggtaaaagt
     8341 gtgctgctgg ggatgatggc acgctacacc cgcgccgatg tcattgtcgt gggtttgatt
     8401 ggtgaacgtg ggcgcgaagt aaaagatttt attgagaata tcctcggtgc cgaaggacgt
     8461 gcacgctcag tggtgattgc cgctccggcg gatgtttctc cgctcctgcg aatgcaaggt
     8521 gccgcctatg ccacgcgcat tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg
     8581 attatggact ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc
     8641 gaaccacctg ccactaaagg ttatccaccg tcggtgtttg ccaaattacc ggcactggtc
     8701 gagcgtgccg gtaatggcat tagcggcggc ggctcgatta ccgcgtttta taccgtgctc
     8761 accgaaggcg atgaccagca ggaccccatt gctgactccg cgcgggcaat cctcgacggt
     8821 cacattgtgc tgtctcgccg actggcggaa gccgggcact atccggctat cgatattgaa
     8881 gcgtcgatca gtcgcgcaat gacggcgttg atcagtgagc aacattacgc gcgagtgcgc
     8941 accttcaaac agctgttgtc gagttttcag cgtaaccgcg atctggttag cgtcggcgcg
     9001 tatgccaaag gcagcgatcc gatgctcgat aaagccatcg ccctgtggcc gcagctggag
     9061 ggctatttgc aacaaggcat ttttgaacgc gcggactgga aagcgtctct ccaggggctg
     9121 gagcgtattt tcccgacagt gtcataaccc aggagataac ggcagatggc ggaagaacag
     9181 ctcaaaatgt tgatcgatta tcagaatgaa tatcgcaata acctcaatag cgatatgagt
     9241 gccgggatga ccagcaaccg ctggatcaac tatcagcagt ttatccagac gctggaaaaa
     9301 gccattactc agcatcgcca gcaacttaat cagtggacgc agaaagttga cattgccctg
     9361 aacagctggc gagaaaagaa acaacgtttg caggcctggc agacactgca ggaacggcaa
     9421 tccacggcgg cactgcttgc agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc
     9481 cagcgcgccg ccatgaggaa acctgaatga ttcgcttagc gcccttgatt accgccgacg
     9541 ttgacaccac cacattgcct ggcggcaaag ccagcgatgc tgcacaagat tttctcacgt
     9601 tgttgagcga agcattagca ggcgagacaa caactgacaa agcggccccc cagttgctgg
     9661 tagcaacaga taagcccacg acaaaaggcg agccgctggt cagcgatatt cttgccgatg
     9721 cgcaacaagc ggatttactg atccctgtgg atgaaacact tcctgtcatc aacgacgaac
     9781 aatccacatc aacaccgtta accaccgctc agacgatgac gttggctgcg gtggctgaca
     9841 aaaatacgac aaaagacgaa aaagcggatg atcttaatga agacgtcacc gcaagtctga
     9901 gcgccctttt tgcgatgttg ccgggttttg acaatacgcc caaagtgact gatgcaccgt
     9961 caaccgtgtt accggcagag aaaccaacgc tcttcacaaa actgacttct gcgcaactca
    10021 ctacagcaca gcctgatgac gcccccggca caccagctca gccattaaca ccgctggtag
    10081 cagaagccca gagtaaagcg gaagtcatca gcacgccttc gccggtgacc gctgacgcca
    10141 gcccgctaat cactccacac cagacacagc cactgcccac cgtcgccgcg cctgttttga
    10201 gtgcaccgct gggttctcac gaatggcaac aatcattgag ccagcatatt tcgctgttca
    10261 cccgccaggg gcaacaaagt gcagagttgc gtctgcaccc acaggattta ggtgaagtgc
    10321 aaatttccct caaagtggat gataaccagg cgcagatcca gatgatttca ccgcatcagc
    10381 acgtacgcgc cgccctggaa gcagcgctgc cggtactgcg tacgcagctg gccgaaagtg
    10441 gcattcagtt agggcaaagc aacattagtg gcgaaagctt tagtggtcag cagcaggccg
    10501 cttcccagca acagcaaagc caacgcacag caaaccatga acctctggcg ggggaagacg
    10561 acgatacgct tccggttccc gtctctttac aagggcgtgt aacaggtaac agcggcgttg
    10621 atattttcgc ctaacgtcag aggtagcacc gtaatccgcg tcttttcccc gctttgttgc
    10681 gctcaagacg caggataatt agccgataag cagtagcgac acaggaagac cgcaacacat
    10741 gactgattac gcgataagca agaaaagcaa gcgatcgctt tggatcccga ttctggtatt
    10801 cattaccctc gcggcctgtg ccagcgcagg ttacagctac tggcattcgc atcaggttgc
    10861 cgctgacgac aaagcgcagc aacgcgtcgt gccctccccg gtcttctatg cgctggatac
    10921 ctttacggtc aatttgggcg atgcggatcg cgtgctttat atcggcataa ccctgcgact
    10981 gaaagatgaa gcaacccgct cgcggctgag tgagtatttg ccggaagtcc gtagtcgctt
    11041 gctgttactg ttttcacgtc aggatgctgc cgtactggcg acagaagaag gcaagaaaaa
    11101 cctgattgcc gagattaaaa ccacactttc caccccgctt gttgccgggc aaccgaaaca
    11161 ggatgtcacc gacgtgctgt ataccgcttt tattctgcga taacgacatg ggcgatagta
    11221 ttctttctca agctgaaatt gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac
    11281 cgacagccag tgttagcggc gaaagtgaca ttcgtccgta cgatccgaat acccaacgac
    11341 gggttgtgcg cgaacgtttg caggcgctgg aaatcattaa tgagcgcttt gcccgccatt
    11401 ttcgtatggg gctgttcaac ctgctgcgcc gtagcccgga cattaccgtc ggggccatcc
    11461 gcattcagcc gtaccatgaa tttgcccgca acctgccggt gccgaccaac ctgaacctaa
    11521 tccacctgaa accgctacgc ggcacagggc tggtagtgtt ctcgccgagt ctggtgttta
    11581 tcgccgtgga taacctgttt ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg
    11641 agtttaccca tactgaacag cgcgtcatca accgtatgtt gaagctggcg ctggaaggct
    11701 atagcgacgc ctggaaagcg attaatccgc tggaagttga gtacgtgcgt tcggaaatgc
    11761 aggtggaatt taccaatatc accacctcgc cgaacgacat tgtggttaac acgccgttcc
    11821 atgtggagat tggcaacctg accggcgaat ttaatatctg cctgccattc agcatgatcg
    11881 agccgctacg ggaattgttg gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga
    11941 actggcgcga taacctggtg cgccaggtgc agcattcaca gctggaactg gtggccaact
    12001 ttgccgatat ctcgctacgc ctgtcgcaga ttttaaaact gaaacccggc gacgtcctgc
    12061 cgatagaaaa acccgatcgc atcatcgccc atgttgacgg cgtcccggtt ctgaccagtc
    12121 agtacggtac tctcaacggt cagtatgcgt tacggataga acatttgatt aacccgattt
    12181 taaattctct gaacgaggaa cagcccaaat gagtgacatg aataatccgg ccgatgacaa
    12241 caacggcgca atggacgatc tgtgggctga agcgttgagc gagcaaaaat caaccagcga
    12301 aaaaagcgct gccgacgcgg tgttccagca atttggcggt ggtgatgtca gcggaacgtt
    12361 gcaggatatc gacctgatta tggatattcc ggtcaagctg accgtcgagc tgggccgtac
    12421 gcggatgacc atcaaagagc tgttgcgtct gacgcaaggg tcagtcgtgg cgctggacgg
    12481 tctggcgggc gaaccgctgg atattctgat caacggttat ttaatcgccc agggcgaagt
    12541 ggtggttgtt gccgataaat atggcgtgcg gatcaccgat atcattaccc cgtccgagcg
    12601 gatgcgccgc ctgagccgtt agtgatgaat aaccatgcta ctgtgcaatc ttccgcgccg
    12661 gtttctgctg agccactgct gcaggtgagc ggcgcactca tcgccattat tgccctgatc
    12721 ctcgctgctg cctggctggt aaaacggctg ggatttgccc ctaaacgcac tggcgttaac
    12781 ggtctgaaaa ttagcgccag tgcttcgctg ggcgcgcgtg aacgggtcgt ggtggtcgat
    12841 gtggaagatg cacggctggt gctcggcgtt accgcagggc aaatcaatct gctgcataaa
    12901 cttccccctt cagcaccaac ggaagagata ccgcagaccg attttcagtc ggtcatgaaa
    12961 aatttgctta agcgtggcgg gagatcctga tgcgtcgttt gttttctgtc gcaccggtcc
    13021 ttctctggct gattacgccc ctcgccttcg cgcaactgcc gggtatcacc agccagccgc
    13081 tacctggcgg tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt
    13141 tgacgttcat tccagcgatt ttactgatga tgaccagttt cacccgcatc attattgttt
    13201 ttggtttatt gcgtaacgcg ctgggaacgc cctccgcgcc acctaaccag gtattgctgg
    13261 ggctggcact gtttttgacc ttttttatta tgtcgccagt gatcaacaaa atttatgtcg
    13321 atgcgtacca gccattcagc gaagagaaaa tctcaatgca ggaggcgctg gaaaaagggg
    13381 cgcagccgct gcgtgagttt atgctgcgtc agacccgcga ggcagattta gggttgtttg
    13441 ccagactggc gaataccggc ccgttgcagg ggcctgaagc tgtgccgatg cgcattttgc
    13501 tcccggccta cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca
    13561 tccctttttt gattatcgac ctggtgattg ccagcgtgtt gatggcattg gggatgatga
    13621 tggttccccc cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg
    13681 gctggcaatt gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga
    13741 cacctgaatc ggtcatgatg atgggaactg aagcgatgaa agtcgcgctg gcactggcag
    13801 ccccgctgtt gctggtagcg ctggtcacgg gccttatcat cagtattttg caggcagcca
    13861 cgcagattaa cgaaatgacg ctgtcgttta ttccgaaaat catcgccgta tttatcgcca
    13921 tcattattgc cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca
    13981 ctaacctgcc gtatatcatc gggtagcccg tacaatgatg caggagacaa gcgatcaatg
    14041 gctttcctgg ttaagcctgt acttctggcc gttactgcgc gtgctggcgc tcatttctac
    14101 cgcgccaatt ctgagcgaac gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat
    14161 gatcacgttc gccattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt
    14221 tgctctgtgg ctggccgtac agcagatcct gatcggcatt gcgcttggtt ttaccatgca
    14281 atttgccttt gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc
    14341 atttgcgaca tttgtcgatc ctggcagcca tctcaatatg cccgttttag cgcgcatcat
    14401 ggatatgctg gcattactgc tgttcctgac atttaacggt catttatggt tgatttcact
    14461 gctggtcgat acctttcaca ccctgccgat tggtagcgaa ccgttgaaca gcaatgcgtt
    14521 tctggcaccc accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc
    14581 gctcattact ctgttgctga ccctgaatct ggcattaggt ttactgaatc gtatggcccc
    14641 gcaattatcc atttttgtta ttggatttcc attaactctg actgtcggca tctctttaat
    14701 ggcggcatta atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa
    14761 tttgctggct gatattatta gtgaattacc attaatataa ttccgtaacg tttatcatgc
    14821 taccctaagg attatccgaa aaataatatc tgcgaacatc ttccaggata ctcctgtagc
    14881 gaaatatttg ttttaagctc actcacatat cgcaacattt actttaagac aattccaggc
    14941 aaattataca acactttacg ggatagtaag tccgcctgaa aaattgcgag agtggcgcat
    15001 taggtgaccc atgttgttcc gtttagtcat gatgaaatat tcaggtaagg ggaattatcg
    15061 ttacgcattg agtgagggta tgccatgtca acgattatta tggatttatg tagttacacc
    15121 cgactaggtt taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac
    15181 attgaaaccg ttgatgacct tgccatagct tgtgattcac agcgtccttc agtggtgttt
    15241 attaatgagg actgtttcat ccacgatgct tctaacagtc agcgcatcaa gcacatcatt
    15301 aatcaacatc ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat
    15361 gaatatctat tggtcagaaa aaatttattg atcagttcta aatcgattaa accggaatct
    15421 ctcgacgata tccttggcaa tattctgaaa aaagagacaa cgataacctc gtttttaaat
    15481 atgccgacgt tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt
    15541 cagggaacca ttcaaatctc cgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat
    15601 aaaggtaata ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc
    15661 cgactgacgg ataatgtgac taatggtatt tttgtcaaca tgcgctaaca cattctgact
    15721 ggtgatttcc caccagtcag gctgaataag attactctgc tttctcaaca aagataccgt
    15781 cctgatgccc tgcttcatta aagaaccaga tgccgagcgg gtagtcttcc agcgcaacca
    15841 ggtacattgt gccttcacta aactcctcaa ctgccagtac cactccagga cgacgcggac
    15901 cgccatccgt tttgactgtt acccgatcat tcaccttcat cgttttcctc ctgtggcttt
    15961 gtgccagtgt agaacaattt cgtttttctg gcagcgccaa gcgcgcgcga gtgctgattt
    16021 tctcgacggt ctatacttaa gagatgctag gcggacttaa cgactggcgg caacaacaga
    16081 gtaacggttg cgaggaaaga tgatgaaaac cgcaaaagtg tacagcgata ccgcaaaacg
    16141 tgaggtcagc gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta gcgaaagcga
    16201 agttcatcgc agccagaacg attctgaaca cgttagcgtc gatggacgtg aatatcatac
    16261 atggcgtgaa ttggcggatg ccttcgaact ggatattcat gacttcagcg tctctgaagt
    16321 gaatcgttga attcacaata aaaaaatccc gaccctgagg gtgtcgggat gaaacttgct
    16381 taagcaagaa gcacttaaaa aattcgttac accaggaaat ctgatgtgtt catcacctta
    16441 tctgcaattt ttttcgctga caagaaaata ttcgcgctat gaatgattaa cgctacttat
    16501 gaatagaaat gtgacgcgcg tcaccccttg gtggctctgg tgtcaacggg atcgctcccg
    16561 aaaaaatcat ctttgattat ttgtgctgtt ttgagaatca tcctggtaag gggagtactg
    16621 cgcgaccatg ttttcaattc aacaaccact actggttttt agcgatcttg atggcaccct
    16681 gctggacagt catagttatg actggcaacc ggcagccccc tggctcagcc gtttacacga
    16741 agccaatatt cccgtcattc tctgtagcag taaaacttta gcggaaatgc tgtacttgca
    16801 aaaaatgttg gggctacaag gcttaccgct gattgcagag aatggcgcag tgatccagct
    16861 tgctgaacaa tggcaggata tagacggttt tccacgcatc atctcaggta ttagccatgg
    16921 cgaaatctgc caggttttaa atacgctacg cgagaaagaa cattttaaat tcacgacttt
    16981 tgatgatgtc gacgatgcaa ccattgccga atggacggga ttaagccgta gctaggcggc
    17041 gctgacgcag ctgcatgagg cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat
    17101 ggcacaattt atcgctcgtc tgaacgaact gggcttgcag tttatgcagg gtgcgcgctt
    17161 ctggcacgta ctggatgcct ctgccggaaa agatcaggct gccaattgga ttatcgcgac
    17221 ctatcaacaa ttgtcaggca gacgcccaac cacacttggc ctgggcgatg ggccaaacga
    17281 tgcgccttta ctggaggtaa tggattacgc ggtgattgtg aaagggctaa accgtgaagg
    17341 ggtacatctg catgatgagg atccggcccg cgtctggcga acgcagcgtg aaggaccgga
    17401 aggctggcgt gaagggctgg accatttttt ctccgcccgt taagcgttat cgctcgcgaa
    17461 tacccgattg cgcccggcct gttttgcgag gtaaagccga cggtcggcca atgactggag
    17521 ttgttcaaaa tcataatcac cggtttcctc gctgctactt acccccagcg aggcactgat
    17581 gcgtatcgtt gtactcttgg cgatcagcat ctctttttca tttaacttca ggcgaatacg
    17641 ttctgcgacc tccgcagcct gcgtcagatt cgcgcctggc agaatcacac aaaactcctc
    17701 accaccgacc cgcccggcaa cgtcctgcgc acgcaaggaa ctgctaatta atccggcagc
    17761 atgagaaaga acacggtcgc ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg
    17821 gtcaagatcg acctgaatga cagaaaaagg atgttggtgc gtctgacaca atttagcgag
    17881 cggacgggct ttttcgaaca gtgcgccacg gttatataaa cgcgttaagg tgtcgtgcca
    17941 cgcctgccac tgcaacgagc tttgcagaac atacatgttg ctgaccatcc ggcgaatcac
    18001 ataccaggag aggagtaaca tggtggtaaa gagcgcccac agcagggtta atgcaatgct
    18061 gatactgccg aaatcgccgc gcacgccttc gcttagcgta tggacgcgcg ccagcacacc
    18121 gtcgaaatgg tccagacgtt cccagctaac atagcgactg tccatacgaa tgccgccccg
    18181 cgtgtcatgt tccattgcct gcgccagcaa ggccagttca cgaggatcaa aaatattccc
    18241 tgttggatgg tcaggattgg aagaggtcaa aaatctcagc ttactgtcat agagctgata
    18301 ctcaccatcg aggtttttat cgatggcgtt tcttaaaaat tgctgcatag tacgcacggg
    18361 aatactcatc cccagcacgc cataccagta gttattactg tctaccggaa cactgacggt
    18421 aacctgcggt tcagtattgc tggcgtgttc cggttgcgaa gtgaaccagc gtaccgcgcg
    18481 gtgacgattt tctcgttgcg aatggccgat aaaccagggt tgggtgacat agccgtaata
    18541 acgcgttggt acattgcgcg taaacaaggt cggctgcgtc gaaacgtaaa atccagcacg
    18601 cgagacatac atcgcctgtt caaccatcga cgaggagttg tgcgccagtc gcagcaagta
    18661 accaacttcc agtgcagcgg taatttcatt gtcgaggctt tcattttcgc gagacaggaa
    18721 attcccctcg ctgactaatg catccgatac gccattgact ggcagggtgc gccgtcggtt
    18781 gagttcgatt tgccaggcgt gctcgtcgcg atgctgttcg aactcggtta cggcattacg
    18841 cagagaggtg aaatccagtg gcgccacgag agcttcgcgc atgccattac gcaaaaagat
    18901 cagtttgtcg acgttatact gcaaatgctt atcgagcgcg ttggctacgt tttccagatg
    18961 attacgctag ctggagatat aggcatcttc cagcaccacc acttcacgcc aggtgagcaa
    19021 ggtggaaaaa agcaatacca cgataaagca gagattaacg acatgaccgg gccccaggcg
    19081 gcgtgcgagt tttttcaacc agctctggtt ttccattttt gtctcgtgct gcaccctgac
    19141 gactcccaac ctgctttttt atgattctgg cataacccgg caatagcgta ccgcaaaaca
    19201 gcgataattt tataatggtg gcatgataag acgcgactat cattcattgc aggcattggg
    19261 acaccaggcc ggatgtgatc cggcctggtg ttaatgatac cctttcaaac ttcttccgca
    19321 gaacgactcc cctcccccgg aacaagatcg gtttcatgaa aagcttcccg cttgaccccg
    19381 taaccgtcat accaacgaca ctcaaccata ccgctggagt atccagtgac aatcatccgc
    19441 gggccgccct ctttaaccgt aacttcctca ctaaccataa agctcatact cgcctccttt
    19501 tttcgagtga aacttgttca ccttagttga agatggcgaa ttttgcataa tagccagtgc
    19561 gagtattaat gtgcctgacc gcgcattttc gctactgctt tcactcccag caccacgatg
    19621 ccgccgatga taaatccaag aatcagattt aaaacagtcg gtaatatcat cgccaccact
    19681 gcactttgct gcccggcgat atgttcaata gcgtgatgca gcggcgcaat accatgtacc
    19741 acaatcccgc cgccgacgag gaacatcgcc agcgtgccga caatcgataa tgctttcatc
    19801 agccaaggcg cgataatcaa taacccttta cctaatgcct gcatcagcgc gctagatttt
    19861 tccgccagcc aataccctag gtcatcaatc ttaacgataa cccctaccag accgtagacg
    19921 cccacggtca ctaccagcgc gatgcctgaa agcaccagca cctgattaag caacggcgcc
    19981 tctgccacaa tccccagcgt gatggcgacg atttccgcag acaaaataaa atcggtacga
    20041 atcgcccctt taattttgtc cttttcaaac ttcagcggat cctgcgccgc cagcttctcc
    20101 agacgctgct ggctctgcgc tggatcttct ttatgtttac gcgcctccag catatgcagc
    20161 actttctcta ccccttcaaa gcagagaaac gcgccaccaa tcatcaacag tggcgtaatc
    20221 gcccacggga taaacgcact gatgatcagc gccagcggca ccagaatcac tttattgatc
    20281 agcgatcctt tcgccacgcc ccagaccacg ggaagttccc ggttggcccg cacgcctgaa
    20341 acttgttgcg cattgagcga taaatcatcc cctaatacac cggcggtttt cttcgccgcc
    20401 agtttgccca tcacagagat atcgtccagc agtgtggcga tatcatcgag caacgtcagt
    20461 aaactactcc cggccaaaag cattccctct catttttatt gttgaagggt gtaagtatga
    20521 agcaaaaacg gcagctctga tacttgcttc acaggtcaac atttatttaa caatcaagtg
    20581 caattatatc tctcgccagc agaatgattt tcacgtttac tatgaaccac ctttttattt
    20641 ccgccgtgag ggattatgcg tttccgccag ttgttaccgc tttttggcgc gctgtttgcg
    20701 ttgtatatca tttggggctc aacctatttt gtcattcgga ttggcgtggg aagctggcct
    20761 ccgttaatga tggcgggcgt tcgattccag gcagccggta ttttattgct ggcatttttg
    20821 ctactgcgcg gacacaaact tcccccgcta cgtccgctgc tcaatgccgc gctgattggc
    20881 ctgttattgc tggctgtcgg taatggcatg gtgacggttg ccgaacatca aaatgttcct
    20941 tccggcatcg ccgccgtagt gattgcaacc gtgcccctct ttaccctgtg cttcagtcgc
    21001 ctgtttggca ttaaaacgcg caaactggaa tgggtgggta ttgccattgg gcttgccgga
    21061 atcatcatgc tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt
    21121 ttaatcggct cgattagctg ggcgtttggc tcagtttatg gctcgcgcat taccttacct
    21181 gtagggatga tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt aatgatcgca
    21241 tcgatgattg cgggtgaaaa actgacggcg ctcccttccc tgtcaggctt ccttgcggtc
    21301 ggctatctgg cgctgtttgg ttcgattatc gccattaacg cttatatgta tttaatccgt
    21361 aatgtcagtc cggctctcgc caccagctac gcctacgtta acccggtggt cgcggtcttg
    21421 ctgggtacgg gactgggtgg cgaaacgctg tcgaagattg aatggctggc gctcggcgta
    21481 attgttttcg cggtggtact ggtcacgttg ggaaaatatc tcttcccggc caaacccgtt
    21541 gtcgcgccag ttattcagga tgcatcaagc gagtaaatga atcccctgcg tgtcgatctg
    21601 cgcgctggca ccttcgccgc agatccactc ttccagacgc tcggtaagcg ccgcatccgt
    21661 cagcttctcg cgcctacgta acgcacactc ccagacaatc agtacgcgcc agccgagctc
    21721 ctgcaagcga ctgatatcgc ggcgatcgcg ttcaacattt ttacctatct tctccagcca
    21781 gaactcagtt cgagtcgcag gcactttaaa cagatagcag tgatgatgat gccagaagca
    21841 gccgtgggta aatatcacgc agcgatattc atcaacgaca aaatccgggc gtccgggcag
    21901 actggcgtcc tgaacgcgaa acgcaaggcc ctgcccggtt aacaaactgg cgaggcgctt
    21961 ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt ttgctgcggg tggccttatc
    22021 gtgaacgtcg accatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg
    22081 gctcaagcag ttttgccacc gcggcaaaga ccggcacgac caccgagttg ccgaactggc
    22141 gataggcctg agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac
    22201 ccattaagcg cgcgcattca cgaggcgtta accgacgtgg acgatgttgc tgattcagcg
    22261 gatcgtcaaa gtctttctca cccgtggcca tatcccaacc gcgatcgatt aaaatttccg
    22321 cgccatcttt gtaataacga gcagacagcg tacgggtgac gctttgcgga ttgttcggat
    22381 aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca tatcgataga
    22441 ggtacttcca cagcaccggc gtcaggatat atttcgcctc aaccatcggg tccagcagct
    22501 gcgccagcgt cactcgctgt gcagggaaac attcgctgat atcacgcaga gtaaaatcgg
    22561 ctttcagatt cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca
    22621 ggaaatgttt accatcgatg attttcggat cgtccggccc gttatcttct gcatcggcaa
    22681 cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg gaacgttttt cccttatcgt
    22741 gactcttcag gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa
    22801 tgcgcaccac atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc
    22861 cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca
    22921 gtaaaacatc gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca
    22981 cgccttcttg atgactgagg gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg
    23041 ggtcgcaata atggttggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa
    23101 acacgcactg tccaccaatc gattcaaaac cacgacggat gccgccaatt ccggcaaata
    23161 gatcgataaa gcgaaacgca taatgcggat gatgttccgg tggtttgggt aacaacgttt
    23221 gcagatgggc aaactctttc tcacttaaac ggtgccatgc cgagtcattc gccagcgcac
    23281 gttttaaaat tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagca
    23341 ttttaacatc ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc
    23401 tgtatgaatc ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc
    23461 cgacaggtta acataattac tgccgtttag ggccacgcgc tcagccagcg ggtgaaaacg
    23521 catctatcac cttgtgatcg ataccttcat tctcccccgc cagttcggca ccgagcttcg
    23581 ccatcaaaca gcgccaagct caatgcccct gaaacggcaa atacgcgcgc caggccaatc
    23641 gcccccaggg cttccagccg atccgcatcc tggacgattt tagcttccgt tgttaagggg
    23701 gcgatttgcg cgctgaaact gtgagcggca atggcatgac aaacggcctc aattttctcc
    23761 gccggaaatt gcacgaactc ttcacggagc aggcgacgcg tctcttctgc cgccaggatt
    23821 gaagaacgct gccgttgcgg atgatttttc gccaggctca cgatatcgtg aaaataacag
    23881 gcggttaaaa tcaccagcat atcgacatcg tcatctgccg ccagtttctg tgccgtagcc
    23941 cagacacggc gaaaatgaca caggtcatgc gccgcgtcct gatgttgatg atggtttttc
    24001 aaccagttct cgaactgtgc ctgccagtgt tgtaactcca tacgcgctcc gttgccaaag
    24061 agtcgcaacc ttagcagttt ttattcatag cgtctgcaac gtgcgggacg aggtcgggtg
    24121 taccatgcaa tgccgcctaa caaggcacca accagggcca taaacagaaa tccgaccaat
    24181 gctcctaacc acttccccgc ggtagatcct aaatcacttt caaatcccga cacgggggca
    24241 tcggggatcc ggctcatcac ccagatgtat cgcgccattg cccagacgaa atagccacac
    24301 catgtataga aagcccac
//
LOCUS       DAEBXD010000058        22896 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEBXD010000058 DAEBXD010000000
VERSION     DAEBXD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22896
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.058"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KJL94_002447"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KJL94_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBD4410764.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KJL94_002448"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KJL94_002448"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBD4410765.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KJL94_002449"
     CDS             complement(3736..4092)
                     /locus_tag="KJL94_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD4410766.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KJL94_002450"
     CDS             complement(4096..4512)
                     /locus_tag="KJL94_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBD4410767.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KJL94_002451"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KJL94_002451"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBD4410768.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KJL94_002452"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KJL94_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KJL94_002453"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KJL94_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBD4410769.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KJL94_002454"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KJL94_002454"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBD4410770.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KJL94_002455"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KJL94_002455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD4410771.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="KJL94_002456"
     CDS             10521..11504
                     /locus_tag="KJL94_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBD4410772.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KJL94_002457"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KJL94_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBD4410773.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KJL94_002458"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KJL94_002458"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBD4410774.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="KJL94_002459"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="KJL94_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="KJL94_002460"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="KJL94_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBD4410775.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="KJL94_002461"
     CDS             complement(15306..15515)
                     /locus_tag="KJL94_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBD4410776.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KJL94_002462"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KJL94_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBD4410777.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KJL94_002463"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KJL94_002463"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBD4410778.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KJL94_002464"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KJL94_002464"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBD4410779.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="KJL94_002465"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="KJL94_002465"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBD4410780.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KJL94_002466"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KJL94_002466"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBD4410781.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KJL94_002467"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KJL94_002467"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBD4410782.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..>22896)
                     /locus_tag="KJL94_002468"
     CDS             complement(22469..>22896)
                     /locus_tag="KJL94_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410783.1"
                     /translation="FIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKL
                     RTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKM
                     LPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNKGI"
BASE COUNT         5895 a         5947 c         5505 g         5549 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaa
//
LOCUS       DAEBXD010000059        22512 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEBXD010000059 DAEBXD010000000
VERSION     DAEBXD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22512
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.059"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..603)
                     /gene="capU"
                     /locus_tag="KJL94_002469"
     CDS             complement(<1..603)
                     /gene="capU"
                     /locus_tag="KJL94_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBD4410784.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
     gene            complement(606..1448)
                     /locus_tag="KJL94_002470"
     CDS             complement(606..1448)
                     /locus_tag="KJL94_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD4410785.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1634..2351
                     /locus_tag="KJL94_002471"
                     /pseudo
     CDS             1634..2351
                     /locus_tag="KJL94_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3024..4532
                     /gene="ltrA"
                     /locus_tag="KJL94_002472"
     CDS             3024..4532
                     /gene="ltrA"
                     /locus_tag="KJL94_002472"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBD4410786.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4613..5100
                     /locus_tag="KJL94_002473"
                     /pseudo
     CDS             4613..5100
                     /locus_tag="KJL94_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5183..5440
                     /locus_tag="KJL94_002474"
     CDS             5183..5440
                     /locus_tag="KJL94_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410787.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(5998..6765)
                     /gene="fecE"
                     /locus_tag="KJL94_002475"
     CDS             complement(5998..6765)
                     /gene="fecE"
                     /locus_tag="KJL94_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBD4410788.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6766..7722)
                     /gene="fecD"
                     /locus_tag="KJL94_002476"
     CDS             complement(6766..7722)
                     /gene="fecD"
                     /locus_tag="KJL94_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBD4410789.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7719..8717)
                     /gene="fecC"
                     /locus_tag="KJL94_002477"
     CDS             complement(7719..8717)
                     /gene="fecC"
                     /locus_tag="KJL94_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBD4410790.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8714..9616)
                     /gene="fecB"
                     /locus_tag="KJL94_002478"
     CDS             complement(8714..9616)
                     /gene="fecB"
                     /locus_tag="KJL94_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBD4410791.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9661..11985)
                     /gene="fecA"
                     /locus_tag="KJL94_002479"
     CDS             complement(9661..11985)
                     /gene="fecA"
                     /locus_tag="KJL94_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBD4410792.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12072..13025)
                     /gene="fecR"
                     /locus_tag="KJL94_002480"
     CDS             complement(12072..13025)
                     /gene="fecR"
                     /locus_tag="KJL94_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBD4410793.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13022..13543)
                     /gene="fecI"
                     /locus_tag="KJL94_002481"
     CDS             complement(13022..13543)
                     /gene="fecI"
                     /locus_tag="KJL94_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBD4410794.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13841..14023
                     /locus_tag="KJL94_002482"
                     /pseudo
     CDS             13841..14023
                     /locus_tag="KJL94_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14017..14121
                     /locus_tag="KJL94_002483"
                     /pseudo
     CDS             14017..14121
                     /locus_tag="KJL94_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14461..14718
                     /locus_tag="KJL94_002484"
     CDS             14461..14718
                     /locus_tag="KJL94_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBD4410795.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERD
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15038..15169)
                     /locus_tag="KJL94_002485"
                     /pseudo
     CDS             complement(15038..15169)
                     /locus_tag="KJL94_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15451..16809
                     /locus_tag="KJL94_002486"
     CDS             15451..16809
                     /locus_tag="KJL94_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBD4410796.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17048..18433)
                     /locus_tag="KJL94_002487"
     CDS             complement(17048..18433)
                     /locus_tag="KJL94_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD4410797.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18483..18830)
                     /gene="tnpB"
                     /locus_tag="KJL94_002488"
     CDS             complement(18483..18830)
                     /gene="tnpB"
                     /locus_tag="KJL94_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD4410798.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18827..19207)
                     /locus_tag="KJL94_002489"
     CDS             complement(18827..19207)
                     /locus_tag="KJL94_002489"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBD4410799.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19562..19996)
                     /locus_tag="KJL94_002490"
     CDS             complement(19562..19996)
                     /locus_tag="KJL94_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBD4410800.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19984..20385)
                     /locus_tag="KJL94_002491"
     CDS             complement(19984..20385)
                     /locus_tag="KJL94_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410801.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20645..21214)
                     /locus_tag="KJL94_002492"
     CDS             complement(20645..21214)
                     /locus_tag="KJL94_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBD4410802.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21959..22102)
                     /locus_tag="KJL94_002493"
                     /pseudo
     CDS             complement(21959..22102)
                     /locus_tag="KJL94_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
BASE COUNT         5422 a         5889 c         5977 g         5224 t
ORIGIN      
        1 ttcgtgccct ttttccgggc gcaacattcc tacctgagca attacggggc atcctcttcg
       61 ggacgccagc caggaaagaa cactcggagg caaactgttt cgggaatcaa cgtataattc
      121 ctggaaatca aagccaggag gcacaacagt aacccgagtt cgacatcctt cctgctccag
      181 atgtgtcttc atatttgttc cgggaacaat cacctcatcg caaaaatgat ttattgaaaa
      241 aaattttgtt tttcgtgtca aatatgtctt ttgcctgata attctgaatg gatgtttcca
      301 ggtaaaaaac cgtaccaaac caacaatatt actatcatgt ccactgtgac aaacgattgc
      361 atcgggctga aaactatgaa ctattccgag taatctccat acagtaggaa tgtgaagact
      421 gtttcgaaat aacgcgaatg tgatatcaat tccaaactta ctggcttcaa aagcaatttt
      481 gctgttctcc ctgcagacaa gcagaactga atgccccatt ttcttcaggg ccttcatttg
      541 agcaacagcc tgtaattcct gaccacctat attcggtgat gattccgtaa atagtatatt
      601 cattattagt catccggtaa tcttggcccc ttatgaaggg ccagcaacga tgaaaaaaac
      661 ggtgttgtat aataaaaaag tcgtcttttc agccatacgc aattttcccg ttccttggtg
      721 cttattcttc ctatccgtgt agttccttta gcaggagcat tcattcttct ttctgttgta
      781 tataaataat ggaatccaag ttcttcagcg atctgaatat aatccttgtt ataataacct
      841 tccggccaac acaaatgttt actgcatttt cctgtcattt tttttaaata ttctctcccg
      901 ctcaataagt cctgccgaag atgtttacac tgttcttctc gtgaagtgaa ctttttatcc
      961 caacgtgtat gagtatgtgt atgaacatga aattcgacca gaccagattg caacatttcg
     1021 ttcacttcag accagcgaag cataacatta tcagcattcc cggttgctat ttgatgttcg
     1081 caatcgcgat gagaatattc ctttccagga gagtgacgaa ccggaccatt tccaataaaa
     1141 ctggtaatta aaaaaatatg tgccttgaga ttaaactcgt ttaataaagg atatacctga
     1201 aaccagttgt caaggtatcc atcatcaaat gtcaacataa cgcttttacg gggaagtttt
     1261 ccgccccgat aaaaaaactc cagttcatct gatgacagtg ttttccagtt gttttcagcc
     1321 aaccatttca tttgtttccg gaatgtctcg ggagaaagtg taaccagtcc tgggcaatgg
     1381 ctgacatgat gatacattaa taccggaaca tgatttgctt taaacaaaat gccccctccg
     1441 tttaaaattt aaagtttctg gcacccagta agtagagata aaaggaaagc ctaataaagg
     1501 tggtgaactt tatatactac taaatcaaac atcaacaatt tataaacaat aatatattat
     1561 catatctttt aaaaacggct cactaagaac cttgaggtgg cacactaaat ttagctacct
     1621 gaacagaggt gatatgctca cctcagaaca acacaggtgc cataatgaaa aaagaaattt
     1681 cagcgcagag tttaaacgcg aatccgctca actggtcgtt gaccagaaat acaccgtggc
     1741 agatgcagcc agcgctatgg atgtcggcct ttccacaatg acgcgatggg tgaaacaatt
     1801 acgtgatgag cggcagggaa aaacaccaaa agcctccccc attaccccgg aacaaattga
     1861 aatccgtgag ctcaggaaaa agctacaacg tattgaaatg gaaaatgaaa tattaaaaag
     1921 gctaccgcgc tcttgatgtc agactccctg aacagttttc gataatcggg aaactcaggg
     1981 cgcgttatcc tgtggccact ctctgccatg tgttcggggt ccatcgcagc agctacaaat
     2041 actggaaaaa ccgtcctgaa aaaccagacg gcagacgggc tgtattacgc agccaggtac
     2101 ttgaactgca tggcatcagc cacggctctg ccggagcaag aagcatcgcc acaatggcaa
     2161 cccagagagg ctaccagatg gggcgctggc ttgctggcag actcatgaaa gagctggggc
     2221 tggtcagttg ccagcagccg actcaccggt ataagcgtgg cggtcatgag cacgttgcta
     2281 tcccgaatca tcttgagcga cagttcgccg taacggaacc aaatcaggtg tggtgcggtg
     2341 atgtgaccta tagtgtgccc ggagttcagg gcgggcatgg atgcttaaat gaaccgcgag
     2401 tctgtctgga atattgaacc ggtaactcac gatgagaaac ccaacaatcc caccgggtgt
     2461 gacggtggag aacctgagcg gcagtgacct gcggcatgcc cgcagggtga tgtaacccgc
     2521 tgacaacggg gattgaggcg agatcactaa gccgagatga tcctcaaggt taagtactga
     2581 aaggctgaag aacatgaacc cgttaatccg cctctgtggg ttgaaaacgt caccacggcc
     2641 tacgtgatct gacaggccgt gcaggaggaa ctggcagtga tacgtaagca ctgccggtcg
     2701 aaggtgtttt gacatgtatg cgaaacaccg gggcagcagc gtctatcacg cttgcgttgc
     2761 tgacttctgc caacttgcgg caagcaagga taaagagtgc gacgggcagc ctcctcagta
     2821 tgcctgagtc caggcaggta aaccggggaa ggtcagcgac ggatgttaag ggggcatggc
     2881 tccgatgacg cgctgactgg cggagcttcc gtagtagtcc gcgatgggga aagcccatta
     2941 catggcgaag ggaagcagtt tgaatgtgtt tgcgacgtga attaactggc ctaacgaggt
     3001 gaagaccttt gataatcagc gaaatgcaac gcaagcttgc cacatgggca gccaccgatc
     3061 cgtccctacg gattcaacgg ctgctgcgtc tgataacaca accagaatgg ctggctgaag
     3121 cggcgcggat cacgctttca tcaaaggggg cgcatacccc cggcgttgat ggcgtgaaca
     3181 aaacaatgct acaggccaga ctggctgttg agctgcaaat cctcagggat gaattactct
     3241 caggccacta ccagcccttg cccgccagac gggtttacat ccctaaaagc aacggcaaac
     3301 tgcgaccact gggtatcccc gcgttgcggg atcgtattgt tcagcgggcc atgctgatgg
     3361 cgatggagcc gatatgggag agtgattttc atacgctctc atatggcttc cggcctgagc
     3421 gcagtgtcca ccacgcgatc cgcacggtga aattacagct cacagactgc ggtgaaaccc
     3481 ggggacgctg ggtgattgaa ggcgacctgt ccagttactt cgacaccgta catcatcgac
     3541 tgctgatgaa ggctgtacgc cgcaggatca gtgacgcacg tttcatgact ctgctgtgga
     3601 aaaccatcaa ggcgggacat atcgatgtcg gtctctttcg ggcggccagt gaaggtgtgc
     3661 cacagggcgg tgttatatcg ccgctattat cgaacatcat gctgaatgag ttcgatcaat
     3721 acctgcatga gcgctacctg agcgggaaag ccagaaaaga tcggtggtac tggaataaca
     3781 gtatccaacg gggccgaagt acggcggtca gagaaaactg gcagtggaaa cccgcggtgg
     3841 cgtactgccg ctatgccgat gattttgtcc tcatcgtcaa aggcaccaaa gcacaggcgg
     3901 aagccatcag ggaggagtgt cggggtgtgc tcgaaggcag tctgaaactc aggctgaaca
     3961 tggataagac taaaatcacc catgttaatg acggctttat ctttctgggg cacaggatca
     4021 ttcgcaaacg cagtcgttat ggcgagatgc gagtggtctc aacgatcccg caggagaaag
     4081 ccagaaactt cgccgcatcg ctgacagcac tgttatcagg caactacagt gaaagcaaag
     4141 tcgatatggc tgaacaactc aaccgaaaac tgaaaggctg ggccatgttc tatcagttcg
     4201 ttgattttaa ggccaaagtc ttcagttata tcgaccgtgt cgtgttctgg aagctggctc
     4261 actggctggc ccgcaaatac cgtacaggta tcgcttccct gatgaggtgg tggtgtaaat
     4321 caccggaacc gggtcagagc aaaacgtggg ttttatttgg taaaaccaat cacggcaagc
     4381 tcagcggcga aatactgtac cggttggtgg ggcaaggcaa gaagctgttc cgctggcggc
     4441 tacccgaagg taatccctat ctgaggacgg agaccagaaa cacgtataca tcgcgcttta
     4501 cagaagtggc aatggcgttc gccagcattt aaatggagag ccggatgcgc tgaaaggtgc
     4561 acgtccggtt cggggaggag aggcagggaa atagtccgac tacgccctgc ctcttactct
     4621 actctggacg ggtaagcgct gggcgtacct cgccgttgtt ctcgacctgt tcgcaagaaa
     4681 accagtgggc tgggccatgt cgttctcgcc ggacagcagg cttaccatga aagcactgga
     4741 aatggcatgg gaaacccgtg gtaagcccgt cggggtgatg ttccaagcga tcaaggcagt
     4801 cattatacga gcaggcagtt ccggcagtta ctgtggcgat accagatcag acagagtatg
     4861 agccggcgtg gaaactgctg ggataacagc ccaatggagc gcttcttcag gagtctgaag
     4921 aactaatggg tgccagcgac gggctatgta agcttcagcg atgcagctca cgcaataacg
     4981 gactatatcg ttggatatta cagcgcacta agaccgcacg aatataatgg tgggttacca
     5041 ccaaacgaat cagaaaaccg atactggaaa aactctaacg cggaggccag ttttagttga
     5101 ccacaacaga ctacctgaag ggagccgcgg tcgcctagca gttgcagtag caggagatca
     5161 tccagccgca gtacaggtca cgatgactct ggttaatgat accggctttg accccgtatt
     5221 ttccggctct atcgctgaat catggcgtca gcagccgtgc acaccatcct attgttgtga
     5281 ctgggaggct gccaccatgc ttcgcgcttt ccctctggcg aaaaagggag aaggacgggc
     5341 ccgtctgcct tcactttatg ccagcttcgg taagctgggt gagacaccga ctcatgaaga
     5401 tatcattgat aacaatcgat ccatcaactg gcctgtataa cgtggctgcc ggtgattaag
     5461 aaagctgcac ctacctaagt agtagcaaac gcacactttt tagaaaaatc gatggtcaga
     5521 aactggatta gcaattccgt tccagggttg cttttgattt acgttggcgt ctgatcattg
     5581 atttatcctc aaaagcccaa cctcattggt aatgaaccag ctccgtgaat gtccgctctg
     5641 gcacagagcg aaattttttg atctcccccc ctgaaatcta aacttagtca tgtcacgttt
     5701 ttgggtttct aaaattttaa cttcgcgttt ttcgttgccg taagggttat acagaaatgt
     5761 ccgttaagca gagttcaaaa ttgattgccg tgatcacgac tggtttgaaa gccgcgccca
     5821 agcctgtaca gctctggttt gcgttgatta tgaacctgtc agcctaaagc aagcggatgg
     5881 acgatgagta ttggtaatct ttcagagtcc ggaaaagttc agccccagtc tgaacaggct
     5941 tgctggcgcc agtccagttt cattcagtcg tggtttggtt cttacggcct gtgcaatcta
     6001 cctcattagg cacatcggcc tgccagatac cggctcgggg tgtatttccg cttccacgct
     6061 gaatactgtt ctcagcaatc ctggggtcat cacctcttct ggtgtgcctt gcgccataac
     6121 atgtccgttt gccattacca ccagttgatc gcagtaccgg ctagcctgat taaggtcgtg
     6181 cagcacagcg accaccgttt tcccctgagt ccggagttcg cccatcaacc gcatcaggtc
     6241 cacctggtga ttgatatcaa gataggtggt tggctcatca agtaatacaa cgggcgtatt
     6301 ctgggccagg accatcgcca gaaatgcgcg ctggcgctga ccgccggaaa gctcggttaa
     6361 ccgacgaacg gcaagatgat tgatccgggt ctggttcatg gcgacattaa ctcgtgcatt
     6421 gtcttcagcg gagagacgcc cccagagtga cagccaggga ttacgaccat acgaaaccag
     6481 ctcctggact gtgatcccct ctggcgttaa atggtgctga ggcagcagcg aaagcctgcg
     6541 ggccaactgg cgtgatgaga gcatatttat gggattatcg ccgagaaata cggtgccaga
     6601 ctgcggcatt aaaagccgcg aaaaacagtt taacagcgtc gatttcccgc aaccgttagg
     6661 gccgatcagg gcggtgatct tccccgttgg cagtgagagt gaaacgtcgt taagtacctt
     6721 gtctgtcccg taactgaccg tcagattttc agttcgtaaa gtcatttatc gcattctcac
     6781 aagcaaccag acaaaccacg gcgcaccgat aatggcggtc agcacgccaa ccgggagctc
     6841 cagtggggga tgaataattc tcgccagcag atcggcaacc accaacagca acgcacctgt
     6901 cagggctgaa acaggcagca gtctgcggtg acgtccaccg gtgatgctac gcatcatatg
     6961 cggcaccacg agaccaataa agctaatcgg gccgcaggcg gccacgccgg tagatgtcat
     7021 ggcgacagct agtaacaaag cccagaatcg ggtatggggc accgacacac cgagcgtggt
     7081 ggcgcgcgca tcgccgagtg caaggaggtc gagatcgcgg caaaaactca ggctcagcgg
     7141 cagaaataaa atcatcagcg ggatggcaat cttcacaaag ctccagtcac ggccccataa
     7201 gctgccggtc agccacagca gggcgttgtt cacatcctgc gggcgcgaga gcatcagata
     7261 atccgtcagg ctggcccagc atgcagaaag cgccacgccg gtgagcgcca gcttcatcgg
     7321 ctggtgggtc tttgccagca tcttcagtaa tatcaacccc gccatgccgc ccgcaaaggc
     7381 cagcagcggc agcaccatca cgggcagtga cggcataaga agtagagccc ccacagaggc
     7441 caggctggcg gcatggttaa cgccgagaat atccggtgat gccagagggt tgcgcacaat
     7501 cccctgtatc agcacgcccg ccacggcgag ggctgcaccg acaaacagtg ccagcagcaa
     7561 gcgcggcagt cggtactcca tcaatacata ataatgctcg cgtccggcct gccagtcggt
     7621 cagcagcgcg cgccacggca cggggatcac tcccatatgg agtgataaca gcgcacagcc
     7681 cgccagggca agggtgatga aaataaccag cgcaattttc atcctcgcct cctcacaagc
     7741 cagacaaagc aagggctgcc aatcagcgcc agcactgcgc ctgcgggcag atctccgggg
     7801 aaggccagcg cgcgtgcgag tacatctgcc agcagcatca gcgtggcccc cagcagcatg
     7861 ctcactggca gtacgttgcg ctgatcgaag cctgcccaga agcgcgccag atgtggcacc
     7921 agcagaccga taaacgccac cggacctgcc acgctgacgc acgcaccaac cagaagcagc
     7981 actaacatat tgatgaccaa acgtagcctc gtcaggttca ctcccagcgt atgggcggtg
     8041 ctgtcgctga ggttgagcag gttcagttga ttcgccagca gtaacacgac agggactgca
     8101 gtgaccacca ccggcaagag ctgccagaca tcctgccagc gggcgtggga cactccgcct
     8161 gccagccagt aaaagatgcc gtaagcatga tcttcggcca gcagcagggt gatgcgggta
     8221 aggcccatac aaaaggccga cagcgcgata cccgcgagga tcagtttgtt tctgtcatgg
     8281 gtatgacgaa atccgcctcc tgcggtcatg accagcagcc agctcacgcc gcccccgcat
     8341 gccgcgatga acgacagaga atagcctgca atcggcgtcg gactcagcgc gctggtaagc
     8401 gccatagcca gcgccgcgcc gctgttaatg ccgagcagtg aaggagaggc cattgggttg
     8461 tgggtcaggg tttgcagcag cgtgcccgcg agcgccaggc ttgcgccgat cagaacggcg
     8521 accaggcttc gtggcaaacg aaggttttgc accagcgctt ctggtagcgt tggcgtgtgt
     8581 ccaggcagca gggcgcgggt tgcatctgct ccggaaacag gaatggccga gtagcaaaac
     8641 agactcagcc agaaaataat aataagtgct gcaacgggaa gcccccacag cagcatcggg
     8701 tgttttatcg cggtcatttc acaacggtaa gcggctgatg gtggaagatt tttaccgtgt
     8761 cagcggcaat acgctctgca gcaaaaatac cgcgcatccg cgcccaggtg ttactgtcga
     8821 ccgaagcaac ctgctgcttc tgcgcggcgg ttaacatctg ccagagcgga tcttgttgcc
     8881 agcgtttaac aatgctctct tcgcgatagt gggcaaccag cagccaggca ggattgaccg
     8941 ccagcagttg ctccaggccg atggacggca tggacgaacc cgccatcgca gcgggaacgt
     9001 tcagccccag agaggccagc acgctgccgg tccaggtctc ctgagtatgc aggttgaatt
     9061 gctgttcgcg tgatgtgcca aaggccacgc gtgtcccttt gggaagctgg ctggcccact
     9121 gcgccatcct ctctttatgt tgttccagac gtgcctgcat ctctcgcttt ttacccacca
     9181 tttcgccgat gatagccgca gattgcaaat tttcagcgta ggtttcgttg cgggacttaa
     9241 gcagcagtac cggcgcgatt tgctgcaagg cgatgtaaac ccccgcatgg cgactgctgt
     9301 cggcaatgat caggtctggt ttcagagcgg caatggcttc caggctcggc tgcgcgcgcg
     9361 ttccgacgga ctgccacggt ttcaggtgcg cacgcacttc gggcaggatg cgttttgcat
     9421 cgttatcgtc ggcaataccg atcgggctga cgtccacggc ggccagcgca tcggcgaacg
     9481 agagttccag caccacaatc cgttgtggcg ttttttcgag tgtaaacgtg ccgtgttcgt
     9541 cctgaaccat ggcggcaaag gcgtggctga tcaccagcag caggcctgca aaaagaaaac
     9601 ggataaatgc caacataatc acattccagc taaaagcccg gcaagccggg cgttaacaca
     9661 tcagaacttc aacgacccct gcatatacag cgtgcgcggc tggcctgcat agatgccttt
     9721 gttgttgtcg tcataagagc ggatgaagta gtcctggtcg aagatgtttt tcacaccgaa
     9781 tgccaggttc agatctgcca tctgcgggcc aaagtcatac gccacgcgtg caccccagag
     9841 catgaagccg ggaatgcggc cggtactgcc gtcggcgctc tctttcaccg tattggcgtt
     9901 atccgcaaac tggctggact ggaaatcgct gttcagattg aacgtccagt ttcctggctt
     9961 gtagtccacg cccagcgtgc ctttatgttt cggggagaat ggtaccagat tgccgtaggt
    10021 gtcgcctttc tcgcggattt ccgcgttcac atacgcatag ctggcgtaga tggaaacgtt
    10081 atcaagcgtt ggcgttagcg tacccagatc gtaacgtgcc tgcgtttcca gcccggtatg
    10141 gcgcgttttg ccacgtgcag tgacggtgtc gttggtctgg ttggagtcgt actgattgtt
    10201 aaagttaatc aggaacagcc ccatttccgc cgtcagcgcg ccgtcgtcgt agcgggtacc
    10261 gagttcccag gttcgcgctt tttccggttc cacattgccg ctttgcacag ccttgccaat
    10321 ctggctgtac tgtacggtgc cgaacgagcc ttcagtgttt gcataaagat tccagctgtc
    10381 agtcaggtga tagagcacgt tcaacgccgg aagcggtgcg ttatagctca cttcttcgtg
    10441 cgtgcctgtg atggcgttgt tctggtatga ctcgatatgt tcgaaacgca tacccggcgt
    10501 gatggtccag ttgccgatgt cgattttgtc atccagatac caggcgtgcg cctcggtgcc
    10561 ggaacgcgta tcgcggtcgt aagggcttga gccggacggc aactgcccgc tgctggtggc
    10621 ggtgtagtaa cgcatttcat gcgttgattc attcacatag cgatagccca cgcccacttc
    10681 gtgcgcggaa gggccgatca taaagatctg gctgtagcgt ggctcaatac cgcgcaccca
    10741 gtagttacgc ggcgagaggg tgatgcgttt gccttgctcc aggtagccgc tgcgcagggt
    10801 ttgggtgtag aacccctgaa tgttgaattt atgctggctg tctggctgga actggtagcc
    10861 caggctcgcc agcttgcgac gaccccagaa gcggtcatac gggcgggtgg attgccagcg
    10921 atcggcgtcg taatccgcgc gagacaggcc accgggcatg tcggcttcac cgtcgtaata
    10981 ttgcagcagg ctgttgaagg tgtgcacctc atccggcgca tatttgcttt tcagcatcag
    11041 gtcgtcgatg cgggtggcgc tgtgctcgcg ccagtcactg ccgcgcgtgc cggagtagag
    11101 cagcgcggtg ccaaaaccgt tgtccgctgt gccgcccacc atcaggttgt gcgtctcttt
    11161 cgggttgttt tgtgaagagg ttgggctgag ctgaccttcc acgcccgcct cgataccaaa
    11221 gtcctgcgga atggcacggg taacaaagtt caccacgccg cccacgctct gcggtccgta
    11281 acgcaccgca ccaccaccgc gtaccacgtc aatggcatcc atgttgccga gcgaaatggg
    11341 agccagtgaa agctgcggct gaccgtaagg ggcgaagggg acggggatgc cgtccatcag
    11401 gacggtcgag cggctggcga ggcgcgggtt caggccccgg atgccaaagt tcatcgccag
    11461 gtcgtggctg ccggtgccgt tgttttccgg cgcgctgacg ccagggatgc ggttaagtac
    11521 ctcacgcatg gtggttgcgc cggttttggc gaaatcctca cggcggatca cgtcacgcgc
    11581 gccagcatgt tcaaatacgt cgttttcacg cgcatcaccc agccagtcgc cgaccacggt
    11641 cagggcatct tcttttggtg cgggcgcggg ctccagcgtc cagctgttat ttcccagcgg
    11701 ttttacctgc agtccgctgc cgtccagcag ttgttgcagg ccgctctcga cgtcgtaatc
    11761 gccgtgcagg ccgttgctct gcttgccgcg cgtcaggctg gcgtcaaccg agagggtaaa
    11821 tccgctgtgt gcggcatact gattgagcgc tttatcgagc gatcccggtg cgatattaac
    11881 ctgtgcagca aaagcggaaa acgagagacc ggccagcggc agcaggctca ggcgaatggc
    11941 gttaaccaaa ggtgttgttt tacgaaaaac gcgtaacggc gtcatacctt ccccatcatc
    12001 atttttgttg tgttcagcta tgagtcgaac gagaagggta aaaaggacaa tcgaaataag
    12061 aattattttc cttacagtgg tgaaatgttt atccagtacc gcgtaataga ctgaatttta
    12121 acgggaagcg tttgcgcgat aacgttcagg atcgcatcgg tatttttcag cgggaacgtc
    12181 ccgctcaggc gcagcccggc aacggcggga tcgcagcgca gcacgccgtt gcggtaacgg
    12241 gttagcgtgg ctatcacctc acccagcggt ttatcgctga agctcaggat gtccttcgtc
    12301 cagcttgtac tctcgtcatc cagcggtttc actgcgccaa actcagaggc gctgaactgc
    12361 aggctttcac cagcgttcac gatgcgtttt tgcgcggggg cactggcgag aagcacttcc
    12421 acagcgtgct gctgaacgtc aagctgcgtg aaattatcct gctggcggac ggtaaattct
    12481 gtccctaaag cagtgagctg gccctgacgg gtcaggacac ggaaggggcg ttgcagggca
    12541 tctttcgcgg tagtaatggc gatttcaccg taccagagcc ggacggtgcg ctgatgcgca
    12601 tcaaaacgca catccgccgc gctttgggta ttcagcgtga gcagggagcc atcttccagt
    12661 tgctgacggc tgactgtgcc tttggcggtg cggtaatctg cccgcagacc ttcgccggtt
    12721 tccgactgcc agagctgcca gcctccgcca gcgccgagca acagcagtaa tcctttcatc
    12781 acgtgacggc gggtgaggcg ggtatcgtgc aacgcccggc tggcaacgtc gccaggcaca
    12841 ccgccaagct ggttgcgcag gttttcaacc tgctgccagg cccactggtt atcctgatcc
    12901 tgttcatacc actgttgcca gcgcgcttcc tgttgtgggc tgacgcgctc gccgcttagc
    12961 acggcatacc agtgggaagc tgaacgcagc gcctgacggc gggaatcggt taacaaagga
    13021 ttcataaccc atactccaga cggaacagca ggcagtgctc gacggctttc gccacgtatt
    13081 ttttcacgga gctgatggaa acaccgagtt tgtgcgcaat ctcgctgtat gtcagaccat
    13141 ccagttgcga aagcagaaac gcttcacgtg ttttgccgtt tagcccgtcc agcatgctgt
    13201 cgaggagttg tagggtctcg agttggcttt cgcgttcctc aggtgaaggc gctcccccct
    13261 ccggcataag cgccagcatc tccagatacg ctttttccag cgcgtttcgg cgaaacaggt
    13321 ccaccatcac gcgtttggcg atagtgcaga ggaaggagcg aggatcgcgg atcgtcgaga
    13381 gcgtttcgct gaccattacc cgcaaaaaag tgtcctgggc aatgtcatct gcatcaaaag
    13441 cagactggag tttgcgcgtc agccagcttt tcaaccagcc gtgatgtgtg ccataaagcg
    13501 actcgaacgt taaggaagct gtggtagtgg cgcggtcaga catgcggagt gcatcaaaag
    13561 ttaattatca cgtagtcata ttaatatgag aatggttatc attacaattg gaaataaaat
    13621 tgtttccaat agacattttt aacatgttgt ttttctaagt gttataaggt aggtataaaa
    13681 tgggatggag cctctgcttc tgacatgtgt cggtcagaat gactcatgat gtggtctgct
    13741 attattgaca tcctcactgc cctaaaggat ggggatttcg gtaatgatct tacccagcaa
    13801 tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca agacaaaaca
    13861 gtttcaacca gtaaaaaaac gtaaacacca ttcgcatgaa tttcgtagtt aagatctgaa
    13921 gcttgcagaa tgcatcggtg ttactgccgg agtccgtgaa cttagtctgc atgaatcacc
    13981 acagctttac atctgtcgca gtaaacagca aaatcagcaa acttacttaa ttatatcgag
    14041 tgcgattaca atcgctggcg atggtacagt tggtgtggcg gtctcagtcc ggaacaattt
    14101 gaaaatcata atctcactta aaacggcatc cattttacaa aagatggccg tctgtgtatt
    14161 tgtaatgaat accttctaca tgtttagagt gcataatcca cgacaaggcg atatcctaaa
    14221 aattagctag caggcaactt tgatggcata gataatgttt ttagtaaaaa agagttgact
    14281 gaatataata cagcctcttc ctacataaga aggaatgtgc tcacaaaaaa aaaccattag
    14341 tctgcttcct gttcttacaa taatcagtat tatgtatttt atttatatag caaaatatgc
    14401 ttgttacacc tgtcataatt aggcatggta aaattattga ctgaaccctg ggggagaaag
    14461 atgcacagtg attattataa tatggttttt ggtgaaaaat tggccaatat tttatatgaa
    14521 gctaactctc aattttttta cgagagaaat gtcatagaag aggcagtcaa cgctttattc
    14581 tgtgagcgtg atataataaa caacaagaac ataataaaga agctgatgtt ctttttgtca
    14641 gatgttaatc acaccaagaa agatgttgtt cagagcgcac ttaatattat cattgacatt
    14701 acatcaggtg atatttgaga ttttacataa aagataatct ctcaaggagt cttatctgaa
    14761 aacgaaatat atagtccctc tcgttttgta atatcgattg gctttgttga atcggttacg
    14821 taccccggag ccggtcatat tttcaggtaa tacacacgct ttcagacatt tcggctttcg
    14881 tcatttttgt atcatgggca tagcttctgc tacctgacca tcataatcac gcagcttcag
    14941 tgaagcagtg accacctgtt taacccggtg tatcgttatg atcttatcca gcaattgaac
    15001 cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga tcatcgtttc
    15061 cgatggaagc ataataagct ttttctgctt ctgccggtgg gatatggccc tgcctttcca
    15121 gcaatcgtcg attgttatac cagtccaccc acgtgagtgt ggtcagctca tcatactgac
    15181 acgtgcatta cccctaccgc gataatgcaa ccgtacaata tagggcgttt cctaaacgca
    15241 gccctcatca ctccaatcca acaagacaca gcatttttga tcacgaagaa agacaaataa
    15301 tttccgtaaa tgtttccgtt gttacaaata acaacagtgt tttacatttc tttggcctca
    15361 ggcccgcgca tgttgccatt cgatgtcatt ttcctgactc gtttcttctt tatggtgtag
    15421 tgtgatcttc actttataga tgctaacaat atgaaaagaa aaattattcc cgtgctaatc
    15481 ggttgtacgc tctctttctc tgccctggcg gcacaaccta cggctgagcg ctatgtcgtc
    15541 agttttccag aaggcacgca cgtgaactac gccggcgcat ttgccagtgc attcccgaac
    15601 ggccttccgg ttgggatagg ttctggcctg ctgttcaccg gtaaacaggg cgatgccctg
    15661 acattcgcaa ccattaccga tcgcggtccg aacgcggatt caccgaaaga ggggaaaaac
    15721 gaaactaaaa ttttcgttac gccagatttc gcgccgctgc tgatgacgat tcgtgtacaa
    15781 aacggcaaag cggaggccat cgatccccgt cctctgcatg acgataaagg cgcgatcaac
    15841 ggtctgccgc tggcaagcga cgtgattggc tccaccaatg aagtggcctt cagcgacacg
    15901 ttacatagac tgaaggggga taatcgcggg ctggatacgg aagggatcac tccggatggt
    15961 aaaggaggtt actggctgtg cgatgagtat ggcccgttcc tgattaacat cgacagtaaa
    16021 gggaaaatcc tggcaatcca cggtccgcag gcggcggaag gggaaaaagc gattgcgggc
    16081 ggtttgccaa atatcctcaa atggcgtcag gcgaaccgcg gctttgaagg cctcacccgt
    16141 atgccggacg ggcgtattat tgtcgccgtg caaagtacgc tggatatcga cgctaagagc
    16201 aagaaaaagg cgctgtttac gcgtctggtg agcttcgatc cggcaagcgg aaaaaccgcg
    16261 atgtacggtt atcctatcga cagcgcggcc tacagcaaaa acagcgacgc taaaatcgga
    16321 gacatcgtgg cgctcgataa ccagcacatc ctgctgatcg aacagggaag agataaaaac
    16381 aacagaatgc gtaacctgat ttacgaggtc gatctcaata aggcgagcga cctgtcaggc
    16441 ttcgacaagc cgggtgagta tccggagttt gatgatgaga aaaccctgtc acagcgcggc
    16501 attacccttg cgcagaaaac gcaggttgtt gatctgcgct ccttgggctg gcagcaggag
    16561 aaggctgaag ggttggcgct gatcgacagc aaaacgctgg cggtggcgaa cgataacgac
    16621 tttggcgtga aggtggcgat gcaacatccg gtcgagggca agacattcaa ggattatcgg
    16681 gtgaacgcgg aaggaaagct gacgcttgac gacaagcagg ttgaaacgac cctcagagtg
    16741 aagccactgg agaaaccgga gtctgacagt gaactgtgga ttgtgacgct gccggaagcg
    16801 ttgaaataag tttataaggt acggacagca gtatcgctta gtttttcaaa ttcatgtatg
    16861 tgaacaatat tggggttccg gcgtagaccc ccaatgaaag cgtcaccacg tgaaaacatg
    16921 cctgcacggt ttacacgtca tcccatggtg gcagccggtt aactttcctg cgccaccagt
    16981 ggaaaaaggc cataagcagt ccgaacaaaa gtaagcgtac agcgagggcc gtattgacgg
    17041 ggatgtgtta ttcagctggc agtgctatgc gccacggaag cagttcgctg acccggttga
    17101 ccggccagtc tgctatgacg ccaagcacat ggcgaaggta gctttctgga tccacgtcat
    17161 tcagtttgca cgtcccgatc aggctgtaca gtagcgctcc ccgctcacca ccatggccag
    17221 agccgaagaa cagggtcttc atcttttcac gcaaccagct ttccagggat ttcaacagcg
    17281 gtttcgtttt tcgctgacgt tcagcaagcc gctgctctgc cggcattccc cttatatccg
    17341 cctctatggc gtacaactga ccgatctgct ccagggcttc ttccgtcagt gctgacggga
    17401 tgcggacgtg cacatcgtgg atctttcggc gggcatgagc ccagcaggca gcttccgtta
    17461 tcccaccatt gcgatacagc tcgttgaacc cggcgtacgc atccgcttgc agcacaccgc
    17521 tgaagcaagc aagatgagtc tgcggatgga tgctttttct gtccgggctg taagcgaacc
    17581 acactgcagg tgccaacgct gacccggcat tgcggtcatc acgaacatac gcccacaacc
    17641 gcccggtctt cgtcttctta ttacccggca gcagtacctg gaccggggta tcatcggcat
    17701 ggagtttgcc gtcagtcatg acatagccat gaagcgcctc ttccagcgga gacagcagcc
    17761 ggcagcatgc atccacccag cccgacagca gtgaacggct cagctccaca ccttgccggc
    17821 cgtatatttc tgactggcaa tacagcgggg tgtgctctgc atacttcgag gtcagcacgc
    17881 gggccagcag ccccggtccg gcgatacccc gctcgatggg ccgcgaaggt gcaggtgcct
    17941 gcacgatggt atcgcactga gtacaggcat gtttttcccg taccgtccgg ataacccgga
    18001 aggcgctacg catcaactcc agctgttcgg cggtatcctc gcccagatag ctcagtgaac
    18061 cgccgcagtt cgggcagcac ggcgccgcag gcaacagtcg cttttcgtca cggggtagtg
    18121 attcagggaa cggcttacgg gtgcgggtct gacgcaacgg acgctgtact gccgggtcat
    18181 acaccctacc agtcagcgta tcgctctctt tctgaagccg gttcagatcg gcttccattt
    18241 gtgcgatacg gcgggagact ttttcggaac gactgccgaa gttcatccgg cggagtttat
    18301 ccagctgcgc ctgcagatgg tctatttcgc gctcccggtt gctcagcttt tcctgcaggg
    18361 cgtggatcag cgcttcctgt tcggccaggc gctgtttcag caggaagatg tcgtcagaag
    18421 agatgtcgtt cataagcccg tattttaccg ggcttattct gtgacaacca ggataaagag
    18481 atttacagca tggtcaggga ggtcagcagc cgcttgggct gtcgccagtc gataccttcc
    18541 agcagcatcg ccagctgcgc ctgcgtaagg aacactttgc catcacgggc tgacggccag
    18601 gcgaagcgcc cacgctccag ccgtttggtc aggaggcaca gtccgtcgcc ggtggaccac
    18661 agcagtttaa cctgactgcc gctgcggccc cggaaaatga aaatatggcc ggacatggga
    18721 tcgtctttca gcgccgtttg tactttcgca gccaggccgt tgaagctatt tctcatatcg
    18781 gtgataccgg caaccagcca aattttggtc ccggaaggta acgggatcat cgcttcagtt
    18841 cctgtatcag cagagtcagg agcttttcgc tgacattgcc attgaagcgg agcgtcccgt
    18901 gccggaacgt tacctcacag ctgatactga gggtttccgg gtcctctgcg agcgattctg
    18961 gctgttcggc agctgcatcg agagtcacag gaagtagctg ggggctctct gaagaaggta
    19021 atagcagctt tccctcgcgc cattgttgtc gccatttgaa caacagattg gcgttaatgc
    19081 cattttcaag agcaagtttt gagatggata tcccgggttc acaggaggca gcaacgagca
    19141 gctgtttaaa ttcgggagga taattagggc agccttttcg cctgccggga gtcacatttt
    19201 tctgcatatc tgacactttg gttcccacta cttatttggt ggacaccact ttgtctaatt
    19261 cgtcagattc tgaccagacg gttcaggctg tacgcttacc actattttat ccctgttgaa
    19321 tttggttaag ccggtgcaga cgcgccggga aacgcacacc tgaaccggca caataaaata
    19381 caatacaacc ccctgttact ggagatacga atggaatgac gaggcagcct gtcagtcaca
    19441 gggggctgtt ggcataaaat caacggaaag gccaccaaca ccggaattaa gctccacagt
    19501 ccccgcactg gcaatccatt cccgggaaag gcatcaccgc aaactgctcc ggaggcaacc
    19561 attacagcct ctgtaacgga ttatcagccc tggcatcttc cgacggcacc tcctgccgcc
    19621 cccacgccct gctgtatacc ccggtaccgc agcgtgggca gcacttttcc ccctcataat
    19681 cttcgtcaca catcacacaa aaccactttc tcaccttcac cgtataatcc ggaaccgtca
    19741 tgccctgcag atgcaaacga cgggcctctt tctctgccgg gtcaagccag gtacatcgca
    19801 gcaggatttt ttcagcgaca gtcatctggt catgttcaaa ccacggtggt tgtccgtcat
    19861 tgccccgctg aagtgttaac gggcatccac aggacacaca gcacaaaacg tcctgtcggt
    19921 tgtgcttcgc agcttctttc gccgtaacaa aatggccttt gctgtcattc gccatataac
    19981 agttcagaat tcgcatattt cctcctgagt tgtccgggga atactgaatc ctccggtctg
    20041 gcagtgtgtg cagtactgct ccccataata atcgtggtga cattgtctgc agtgccagct
    20101 ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt taatcaactg
    20161 tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg tcagcctgtc
    20221 gtcagtgtgt tcaaaccagg gaagttcagt ttcgtattgc ggatggtatc tgagtgcact
    20281 gccacacaag tggcaggtgt agttagcata aggtgcggcc tgtgcagtac gggcgcccgt
    20341 cagacgtccg ttgccatcaa gagcgataaa ggattttgcg tacatagtca tatacctcag
    20401 ccgccagacg acacgcaggc aacagccgtc ccggtacggg cagcgtggtc agggtgtgaa
    20461 tggcggttca gttaaaatct ggactggagt ttgggtaaag caggctggtt gctgttacag
    20521 cactgtggtt gtcacacaat ggaacactga cgtaaaaaac agtcagtggg cgagtggata
    20581 catcgttgcc gccagacaac aacccatact tttggtttgc cggatattgg cgcgccagtc
    20641 gtgctcaact ccggctacag ccaaaattgc gctcaccggt gcagtgagct ttggtatgct
    20701 ccttcgccag atagtcagca cgttccagca cttgctgaaa gccggtgtca tcattacgcg
    20761 ccagccacac cgccggccag gccggaaaat gcaccagcgt ggcatggcac ccgacatcca
    20821 ctcccagggc actgcaccac gcctgtttaa tcatcccggc cagagactcc ggagcgcggt
    20881 aatcaccggc acgacaccag gtatcccggt tgaccagcag catcaggtga tagtgttttt
    20941 tacctttgca ctccccaaac tcccgggccc agacgtaatg cagggttgtg ggatgcacgc
    21001 gtttaccttc ccgatgctta cgtttctggt aagcgtcgat tcgggctttc agggcattga
    21061 tgaagcggga tatcacagct gcgtccgttg ctgccggtac atccgggaga cgcaggtcaa
    21121 cccgcaatgc cgtcaggcgg ggatgaacat tcagtgcgtg ccgcaccgtc tcacgaatac
    21181 gttgctgcca gaaggggttg tatttgtagg tcatggttaa atctccgtat ggttcatacg
    21241 gaatagccac gtcgtaaaaa atacgcagag tccctgaagt gacctccggc aggacactgt
    21301 ctcaggcact ctgtgataac ccgaacggtc gtctccggac tgacggttga attttcagcg
    21361 tcgtttttta atgtaaaaaa ccggttacgg gtaaggctgt gaggagggag tgatggtgat
    21421 acgcaaaaag aagtgcaggg actgtggaaa cgcgattact cataatacag tgtgttgccc
    21481 gtattgtggt tctgtcgacc ccttcggcta ctaccggaat acagaccgta tcgttaccat
    21541 actgctggcg ctgattattg tggtcctgct gactactgtc agcgtcagtg tgtgtatact
    21601 gtgtagctgg tgagataacg gttcatgtat cccctgagct gacagtgtac cgtgtatatt
    21661 aagcgtaacc gacaggttct ccttcatacc atattaaatt ttccagggat tgccgctgcc
    21721 ttatttcgtc ccatacagga aaactcatct gggtttctga caaatacgcg aaactcacaa
    21781 cccctcaata tcaaccacac gttgtaagcc ggtaatacct gaaaatcagc ctattaccgc
    21841 ccacgcctcc ggcaccattc ccttttgatt tcgaattctt tgttgtcgct ataccgctta
    21901 atgttcccgg tcctccggtg cagataacac cggagtcatc gtagcgctgc ttcaatattc
    21961 aggtgacgta tttccagacg gaagccggcg cgcgatcgta aagtttgttc attgtcagtt
    22021 ctgccagccg gttgtcaacg gcagagttaa aggcttccag ctccgcaagg gtaagtttat
    22081 aacgcgtgtg ggaattcact tttcccgcgt ctcctgacgt attacgggct gccacttacc
    22141 tggtgtcccg caatacagcc ttagtgttcc gttatcgggg atccggacgc accgcaacaa
    22201 ccggggaagt atttctggtt tagagcagtg tcaagaggga tttctcagcc cctcactcac
    22261 cggctcagtc tcagggcagg agcattaata cgctcagttc atgtcttata caggaatggg
    22321 cacaggggga gctggcgtgt cactgctgat tttacgcatc agcgttcatg tatctgcact
    22381 ctgatacatg aacgattcac agcgctctgt taccggagag atttactctc ttagcttact
    22441 ctggcaaatc ctttacgtta ctctctgatg acttcttata tttataatac caatcagaca
    22501 gccttatccc at
//
LOCUS       DAEBXD010000060        22159 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEBXD010000060 DAEBXD010000000
VERSION     DAEBXD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.060"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KJL94_002494"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KJL94_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBD4410803.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="KJL94_002495"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="KJL94_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBD4410804.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="KJL94_002496"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="KJL94_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBD4410805.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KJL94_002497"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KJL94_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBD4410806.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="KJL94_002498"
     CDS             3251..5011
                     /locus_tag="KJL94_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBD4410807.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="KJL94_002499"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="KJL94_002499"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBD4410808.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KJL94_002500"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KJL94_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBD4410809.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KJL94_002501"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KJL94_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBD4410810.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KJL94_002502"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KJL94_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBD4410811.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KJL94_002503"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KJL94_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBD4410812.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="KJL94_002504"
     CDS             complement(9679..11586)
                     /locus_tag="KJL94_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4410813.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KJL94_002505"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KJL94_002505"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBD4410814.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="KJL94_002506"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="KJL94_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBD4410815.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KJL94_002507"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KJL94_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBD4410816.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMLLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KJL94_002508"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KJL94_002508"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBD4410817.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="KJL94_002509"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="KJL94_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="KJL94_002510"
     CDS             complement(17598..18113)
                     /locus_tag="KJL94_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4410818.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="KJL94_002511"
     CDS             complement(18121..18663)
                     /locus_tag="KJL94_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4410819.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="KJL94_002512"
     CDS             complement(18761..19744)
                     /locus_tag="KJL94_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4410820.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..22107)
                     /locus_tag="KJL94_002513"
                     /pseudo
     CDS             complement(19735..22107)
                     /locus_tag="KJL94_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5495 a         5803 c         5204 g         5657 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgaact ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac agcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaatggt aatgactcca acttactgat agtgttttat
    22141 gttcagataa tgcccgatg
//
LOCUS       DAEBXD010000061        21722 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEBXD010000061 DAEBXD010000000
VERSION     DAEBXD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21722
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.061"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..120)
                     /locus_tag="KJL94_002514"
     CDS             complement(<1..120)
                     /locus_tag="KJL94_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD4410821.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIF"
     gene            complement(113..1003)
                     /locus_tag="KJL94_002515"
     CDS             complement(113..1003)
                     /locus_tag="KJL94_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBD4410822.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1033..2289)
                     /gene="wzx"
                     /locus_tag="KJL94_002516"
     CDS             complement(1033..2289)
                     /gene="wzx"
                     /locus_tag="KJL94_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBD4410823.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2305..2850)
                     /gene="rfbC"
                     /locus_tag="KJL94_002517"
     CDS             complement(2305..2850)
                     /gene="rfbC"
                     /locus_tag="KJL94_002517"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBD4410824.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2855..3733)
                     /gene="rfbA"
                     /locus_tag="KJL94_002518"
     CDS             complement(2855..3733)
                     /gene="rfbA"
                     /locus_tag="KJL94_002518"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD4410825.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3792..4691)
                     /gene="rfbD"
                     /locus_tag="KJL94_002519"
     CDS             complement(3792..4691)
                     /gene="rfbD"
                     /locus_tag="KJL94_002519"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBD4410826.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4691..5776)
                     /gene="rfbB"
                     /locus_tag="KJL94_002520"
     CDS             complement(4691..5776)
                     /gene="rfbB"
                     /locus_tag="KJL94_002520"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD4410827.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(6149..7042)
                     /gene="galF"
                     /locus_tag="KJL94_002521"
     CDS             complement(6149..7042)
                     /gene="galF"
                     /locus_tag="KJL94_002521"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBD4410828.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7217..8611)
                     /gene="wcaM"
                     /locus_tag="KJL94_002522"
     CDS             complement(7217..8611)
                     /gene="wcaM"
                     /locus_tag="KJL94_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBD4410829.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8622..9842)
                     /gene="wcaL"
                     /locus_tag="KJL94_002523"
     CDS             complement(8622..9842)
                     /gene="wcaL"
                     /locus_tag="KJL94_002523"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBD4410830.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9839..11119)
                     /gene="wcaK"
                     /locus_tag="KJL94_002524"
     CDS             complement(9839..11119)
                     /gene="wcaK"
                     /locus_tag="KJL94_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBD4410831.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11299..12777)
                     /gene="wzxC"
                     /locus_tag="KJL94_002525"
     CDS             complement(11299..12777)
                     /gene="wzxC"
                     /locus_tag="KJL94_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBD4410832.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12779..14173)
                     /gene="wcaJ"
                     /locus_tag="KJL94_002526"
     CDS             complement(12779..14173)
                     /gene="wcaJ"
                     /locus_tag="KJL94_002526"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBD4410833.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14228..15598)
                     /gene="cpsG"
                     /locus_tag="KJL94_002527"
                     /gene_synonym="manB"
     CDS             complement(14228..15598)
                     /gene="cpsG"
                     /locus_tag="KJL94_002527"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBD4410834.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15791..17227)
                     /gene="cpsB"
                     /locus_tag="KJL94_002528"
     CDS             complement(15791..17227)
                     /gene="cpsB"
                     /locus_tag="KJL94_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBD4410835.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17230..18453)
                     /gene="wcaI"
                     /locus_tag="KJL94_002529"
     CDS             complement(17230..18453)
                     /gene="wcaI"
                     /locus_tag="KJL94_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBD4410836.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(18450..18929)
                     /gene="gmm"
                     /locus_tag="KJL94_002530"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(18450..18929)
                     /gene="gmm"
                     /locus_tag="KJL94_002530"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBD4410837.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18929..19887)
                     /gene="fcl"
                     /locus_tag="KJL94_002531"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(18929..19887)
                     /gene="fcl"
                     /locus_tag="KJL94_002531"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(19890..21011)
                     /gene="gmd"
                     /locus_tag="KJL94_002532"
     CDS             complement(19890..21011)
                     /gene="gmd"
                     /locus_tag="KJL94_002532"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBD4410838.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(21037..21585)
                     /gene="wcaF"
                     /locus_tag="KJL94_002533"
     CDS             complement(21037..21585)
                     /gene="wcaF"
                     /locus_tag="KJL94_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBD4410839.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21601..>21722)
                     /locus_tag="KJL94_002534"
     CDS             complement(21601..>21722)
                     /locus_tag="KJL94_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBD4410840.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5421 a         5728 c         5008 g         5565 t
ORIGIN      
        1 aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt cctcaatcca
       61 gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag aactatgcat
      121 tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa atatgttttt
      181 tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga attaaagaaa
      241 aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc aaaagaaatg
      301 catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta atcccacaga
      361 tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg atatgaacta
      421 atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca ataaaaagtt
      481 cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt ccggatgaga
      541 ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc tctcgaagac
      601 catttttttt gatattacaa ataggataat aatgtgactt atctctatca ataaaaactg
      661 ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg ataaactcat
      721 tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt tcaagagaat
      781 atcgaatccc aatattttgt gcttttgcta taccttcatt cctaccaaga caaattaaat
      841 gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta ttatcgacta
      901 ctacaacgta acaattttgc tcctttagtt cagtgatcag tgcattcaga tttttaagtt
      961 cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa gtgactcagt
     1021 aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc attgtaataa
     1081 cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta acccatattt
     1141 ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat gaatcaaagc
     1201 agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg ccagcgcatt
     1261 gaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt tataattctc
     1321 accaaaccat attttcaata caagccctga atataacaag cctatgatta tcaccggcaa
     1381 acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt ctttaaagtc
     1441 tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta ttattcctaa
     1501 ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc ccataatatt
     1561 tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac ttactgtcat
     1621 ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa cccccgaaat
     1681 tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc ttgcaaatat
     1741 taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg ccggaattcc
     1801 cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt tttcatcccc
     1861 ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa tagccaacag
     1921 tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat aattaacaat
     1981 tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca ccaaaaaaga
     2041 tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa ttgcaatttc
     2101 tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa caagtgcaat
     2161 tgcaattgta taaataccga aaccttcagg ccccaaacct cgagcgagaa atcctagcga
     2221 ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac tattttttat
     2281 tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa atttttgacc
     2341 attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat ttatactttt
     2401 gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg ttttgtagac
     2461 aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg gtatccataa
     2521 ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg ttggtgatga
     2581 tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta ccagttttcc
     2641 ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag ttgatttaga
     2701 atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa ctttctgatt
     2761 gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa aaattagcac
     2821 atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc attttcagca
     2881 gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact tgctcagcat
     2941 caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc ccctgacgct
     3001 cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc cccgtgtcca
     3061 gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc atataaatac
     3121 ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt ttcgccattt
     3181 ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt tttggttcca
     3241 gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc acaccgtagc
     3301 gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg ttaacagcgg
     3361 tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt accagcgcac
     3421 aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga ccatccggac
     3481 tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca agcaattgtt
     3541 gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata cccgccaaca
     3601 taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc agttgtttgc
     3661 tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga ataatacctt
     3721 tacgcgtttt catttcacca ttccttttaa ttcatctcgc tctagcatca tgagcgagat
     3781 gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg tttcacgccc
     3841 acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt attgaggcga
     3901 gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc attaagtttg
     3961 ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc ggcataatcg
     4021 tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc tggtttattt
     4081 gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc cgttggtgcg
     4141 ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg caacatcgtt
     4201 ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat aagatgcttt
     4261 gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta gacatttagc
     4321 ggtgctgttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa aacataatca
     4381 gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat cgcttcgacg
     4441 catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc tactgcagtg
     4501 tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc agccacacct
     4561 tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc aatcaagtta
     4621 cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt gccgaaaagc
     4681 aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga taagcaccgc
     4741 ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt ttacgaatcc
     4801 cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta tcggcatcaa
     4861 tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc tcacgataag
     4921 atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact acgtcgatgt
     4981 ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct tcggttacga
     5041 cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt tgattccctt
     5101 taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat ggaataagtt
     5161 tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg gttggtaaac
     5221 cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac gcagaatacg
     5281 ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg ttattatttg
     5341 cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga tgaaagcgga
     5401 aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct tctaaaagaa
     5461 cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt gaacggtcaa
     5521 cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg aaaatacgcg
     5581 acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca gaatcagaaa
     5641 catcagcgag tgattccagg ttcccggcgt atgttaattt atcgacatta acaacactat
     5701 cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct gcgccaccag
     5761 taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg aaatagactg
     5821 ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt aacagctacc
     5881 agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc aaatccgaaa
     5941 aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc ctggaaaaat
     6001 ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa cggcaacggc
     6061 aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat aattttctta
     6121 tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca atacctttac
     6181 ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc tgcatatagc
     6241 ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca acggattgtt
     6301 ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca ccaggctgag
     6361 tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg gccatgatgt
     6421 ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaatg cggctgactt
     6481 taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag aggtcacccg
     6541 gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca atcatggcag
     6601 caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct ggcagtacca
     6661 cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg cctaaaccta
     6721 aaggttcgcc ctgacgcacg ttcataatga tcacgcccgg cggacagatg gactgtactt
     6781 ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca taagaggtgt
     6841 cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt tctttgatcc
     6901 ctgcagccac aatctcgtca acaatgtact gaatcattgg cttgtcgacg attggtagca
     6961 tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc gctacaggaa
     7021 taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct gaattatagc
     7081 ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat tacttgtgat
     7141 gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct ccatttttcc
     7201 aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc ttttccacat
     7261 taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt tcattcaccg
     7321 catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg ccgcgaacgt
     7381 ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc tgcatcacat
     7441 tgatatttcg cataaaaaga tgttgcggct tgttgtgtaa ctccagcgag gcacgcttca
     7501 tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt tggatgccgc
     7561 gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg aaattttgcg
     7621 gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg gcgctattaa
     7681 tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact gtcgcgttat
     7741 caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata ttacgaataa
     7801 caaaatgttt accattttca acatggatca actgccgaca atccgatccc gtgatattcg
     7861 ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa gtgcttcccg
     7921 caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata cgctcgatga
     7981 catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca atggcgtcgc
     8041 cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg ttatgaaatc
     8101 cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg gtcaggttgc
     8161 gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca aagccgctca
     8221 tcgccagccc tttgatggtg cagtcagaac aacgcacatc cagggtaatg ttatgcatac
     8281 cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc cctctgccgt
     8341 tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag atgccggtgt
     8401 tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg ctgaaggctt
     8461 gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg ctttgtcggg
     8521 cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt gccgtcagga
     8581 aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc ctgcagcaag
     8641 ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt ttcgcgcgca
     8701 cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc cagacgttgt
     8761 gccagtgcgc gagcatcgtt ctcaggcagc agccagccgg atttatcggc ctccaccagt
     8821 tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat cgcttccatt
     8881 agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg caacaggaag
     8941 acatccgcgt cgtcgagcat ctccttcact tcatggctcg gtttaaagcc cggcatctct
     9001 accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc ccacaggcca
     9061 atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg gcaggcttca
     9121 atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat ttccagcggc
     9181 gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc catacgcgat
     9241 acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca cagatcgctt
     9301 atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt gtagtggttg
     9361 agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt gccgcgaatg
     9421 atacccagtt cgcgtagttt tgctgcggtt acccccgctg ggccaaaatg agcgatgaac
     9481 acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat caggttccgc
     9541 gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg atgaatgcca
     9601 cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcccgcagg ttcgtcctgt
     9661 aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt gttctctgta
     9721 tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa cgcggtaatt
     9781 tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa gccgaccttc
     9841 atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc gtctgacgtt
     9901 cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct aaggtatccg
     9961 caaccatcgc ttgcaggctg ccgtccagta aatgacgaat atcaatcgcc atctctggca
    10021 gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca atcgccggag
    10081 tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg agttcacagg
    10141 ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg tggtaacggg
    10201 caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct ttgttatagc
    10261 tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg agaatgcgat
    10321 tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga cgtttgtcga
    10381 acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca acgtccagcc
    10441 agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc agccacgcgg
    10501 tatcgacgcc atgttccact tttgcggtgg tgatatttct gcgtttcatc aaatcaaggc
    10561 tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc gccagttggt
    10621 taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc ggctttttcg
    10681 ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg acaaaaaacg
    10741 atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag tcggtgaatc
    10801 cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag agcaatacct
    10861 gatgctggta gcggcgacgg aggacttttt taatgcgccc gacaacgccc gccgcgctat
    10921 tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg agcagccagg
    10981 aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg aggatgttga
    11041 tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa gtgtggttgc
    11101 ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg ctgtgtttgt
    11161 tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag gcctacaggt
    11221 cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc aaccgttgtc
    11281 ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt cgctgcgaca
    11341 aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca tcaccacaaa
    11401 cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca gttgcccttt
    11461 cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat aaaacggcag
    11521 ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa tcatcacgaa
    11581 gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga cgccgattgc
    11641 gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt tgaatacgtt
    11701 gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa tcgggttacc
    11761 cacggagcgc agcagaccca caatacacag caattgcagc accggaataa tgctgttcca
    11821 cttttcgccg aataccagcg gtacgaagtt attcgacacc accatcagcc ccagcagcgc
    11881 cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac gcagcttttc
    11941 ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga ttgggttcag
    12001 cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg ccacgcccgc
    12061 gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt tgatgatgct
    12121 gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca gcgagaagtg
    12181 cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc tcaccgcact
    12241 attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg cgctaactac
    12301 cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt tgttgaactc
    12361 cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca caaacgccag
    12421 cgacaatgtt ttaatcaacg gtgccaagtc cgggttattc agcacgtcgc cgatgacatc
    12481 actcaacaaa aacaccgcca cacataccac aagccccagc ccgacgttca gccagtacag
    12541 cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag cgataccgaa
    12601 gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca ggccgaactg
    12661 gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc cgatgatgat
    12721 caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta agctcatatc
    12781 aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt ttgatatcga
    12841 accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt tccattttct
    12901 ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta atgcccggtt
    12961 tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg ttatgcgcta
    13021 ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg ataaactgcg
    13081 gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg cgcggatcgt
    13141 tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa cggaacttcc
    13201 acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata accggccctg
    13261 gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc agcagcagaa
    13321 taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaacc cccgaaagcg
    13381 gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt gaatggagaa
    13441 tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc gccagttgat
    13501 ggaccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag acgttatgaa
    13561 tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag tcgttagaaa
    13621 cgccgcccgg tttcgggtcg tggtaaacgc ccaccacttc aaaccctaac cacggctgat
    13681 tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccactacg gcgaccatcc
    13741 gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat gaacggcaaa
    13801 ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt tgcgtgtcga
    13861 aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc cagttttgta
    13921 gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa aaatcggtga
    13981 tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg tgcatgtaga
    14041 ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg atggtgatat
    14101 ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc tcgcgctttt
    14161 ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg taagggaaga
    14221 tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc atcagcggca
    14281 catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag gtgcgcaggt
    14341 taaagcacca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc gccagcgcct
    14401 cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc agtttgctgt
    14461 tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg cccagcgttt
    14521 ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg ctgtcgcagt
    14581 aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg gcgtcttcct
    14641 tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc gtgccgcctg
    14701 cagcagtcac cacatcaata gtgttccaag agagacgcgg atcatggatg atcttcgcgc
    14761 cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag ccctcgataa
    14821 actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag gcaatgccca
    14881 tatccgcgcc gtgtttgatg accgcattgc gggtgtcgtc gcgacattcc ggcagcagcg
    14941 ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg attaattcca
    15001 cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga cccgctgcgc
    15061 cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg atataaccga
    15121 acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg cgtttggttt
    15181 catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc agtccggtat
    15241 caccgctgat cgtgcgagcc ccctcgcgca ccagcttcat gccgttgtaa tccatcggat
    15301 tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg gcgaaataga
    15361 tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc tgtaaacctt
    15421 ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct aacacaatgg
    15481 ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata tcttcattca
    15541 gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat tttttcatgt
    15601 cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt atttttgtcg
    15661 ttatttacac cgcggtttcg cattcattgc ctgatgcgac gctgccgcgt cttatcaggc
    15721 ctacaaaacc gaaccgtagg tcggataagg cgttcacgcc gcatccgaca aacagcgcct
    15781 gatgcgacgt ttacacccgt ccgtagcgat ccgcgaagcg caccacatca tcctcttcga
    15841 gataagagcc ggagcgcact tcaattaaat cgagcggaat tttccccggg ttttccaggc
    15901 agtgcgtcgc ccccagcgga atataaatgg actcgttttc accaagcagt ttgatatcac
    15961 catcaatggt gacttttgcc gttcccgcga ccactaccca gtgttccgcg cggtgatggt
    16021 gcatctgtac cgacaaaccc tcgcccggtt tcacggtgat gcgtttcacc tggtagcggt
    16081 cgcccgcgtc gatagagtca tatttgcccc acggacgata cacttcgcga tgcacccgat
    16141 gctcatggcg accatcggct ttgatctgct cgaccacttt tttcacatcc tgtaccgcgt
    16201 tacggtcggc aatcagcacc gcatctttgg tctgcaccac taccagatct ttcaccccga
    16261 cggtggtgac caggcctgat tcggcataca catagctgtt ttcagttttg tgattaatca
    16321 catcgccgtg gcaaacgttg ccctcggcgg tgtgggcgct gatctcccat aatgaagacc
    16381 aggaaccaac atcgctccag cccgcatcca tcggcaccac aacagcatct gccgtaggtt
    16441 ccatgaccgc gtaatccacc gactcttccg ggcaggcgag aaacgcttct tcatccacac
    16501 gaataaaatc gagatccgga tcgacggcgc tcatcgcttt ttcgcaggca tcgaggatat
    16561 ccgggcgata ttttttcagt tcttcgagat agcgtccggc gcggaacagg aacatgccgc
    16621 tgttccagta atattcgccg cttgctacat aggcctgggc ggtttccaga ttcggttttt
    16681 cgacaaactg cgccacttca aaggccaccg tatcctgctc acccacagac acttcaccgc
    16741 gacgaatata gccataaccg gtttctggta gatccggcac aatgccaaag gtcaccagct
    16801 tgcccgcttc ggcataaggc atggcattac gcacggcggc acggaacgcg tcttcatcgg
    16861 caatcacatg atccgccgcc aataccagca ttaacgggtc gctctccggg ctatgacgtt
    16921 ttgccgccag cgccgccagt gcaatggcag gtgcagtgtt acgccctgcc ggttcgagaa
    16981 taatgttctc ggtgagtttg ttcagttgac gcagctgttc cgcgacaata aagcggtgct
    17041 gctcattgca aatcaccacc gggctttcgc actccacgcc gttcaggcgg cagatggtgg
    17101 tttgcagcat ggtgagatcg cctttcaggc ataaaaactg cttggggtaa agtacgcggg
    17161 aaagcggcca taaacggcta ccggagccac ctgccatcac aactggatag agtttcgact
    17221 gcgccataat tatccccgaa tatcatttat aaattgacgt aacacgttct ctttatcgag
    17281 cgtgcgttcg gcatattcac gtgccaccgt gttatgtttg ggcagcagga gcgcctgacg
    17341 aatccccgcc accagcgcct cgaccgattc cggttctacg caaaccgcaa tacccggaaa
    17401 ggtttcgcaa agctgcccca gttctgtgtg ggcctctgcg gtaatcaccg cgttaccgcc
    17461 taccgccaga atattggtca gtttcgacgg caatacggca tctgccgcgc cgcgtttttg
    17521 caccaccaga tggcaatcgc ccatcttcag cagtgcaggt aaagcgtcat acgattgcag
    17581 cgggaaaaat tgcatgttgc gcagtccacg ctgctgcgcc attttttcca gccgcgcttt
    17641 gccgccgctc tgcccgacaa tggcaaaaat cagcggttca tcgcgcagga gatcggcagc
    17701 ttcaataacg ttttccagcc cctgcttttc accaatattg ccggagtaaa gaatgatttt
    17761 tttgttatcc ggcaggccaa gctggttacg aagggcatca acatcggcgt ctgcaacatg
    17821 ctgaaaacgg gcaatttccg accagttggg gaagaagatg acgttatccg ccgccacgcc
    17881 tttttcgatg gctttattca tcatcgaacg cgaaatcgtg gaaacgttat cgacgttatg
    17941 cagtccgcta cgttcgaacg ccgttgccag ctgtgccact ttgccgcctt tgccttttcc
    18001 ggcaaggccc agccccagca tggcgtccac ttcgtaatcc tgaatatgca gcacggtacg
    18061 cgcaccagag agcttcgcca gcaggcgcat tcccggcgtg caaaacagcg ttggcactac
    18121 gccaataatg cgatccggct tccagcgacg ttgcgccatc agtggaaaga aactgctgac
    18181 ggcaaaactg ccgagatgca acaagcgttt cagggtgctc ggctgttttg gcacgtacag
    18241 cgggcagcgc cacaccgtgg cagccccttc ttctcgtttg tagcgccagg cggaatagtt
    18301 ctcacccacc tgccattgcg ggtagtaagg cggtgcggta atgacccgca cctcatgacc
    18361 ttgtgccgcc aaccattcca ccatctcgcc ggtgtatttg ccgatgccgg ttaactccgg
    18421 cgagtagtta atgccgtaga ccagtatttt cataatccgg gtactccggc acgcttctca
    18481 gcgagaaaat aggctcggct gttagcgtga acatcgttac tggcgagcag cgcgtccggc
    18541 gtcagccagc ggtaatcgtc atgctgctca tccggcagta acagctcttc ttccgctact
    18601 ctgaagcgaa aaccgagcac cacatagtga gtggtgaaat ccgtgccgga gaagttatcg
    18661 tcataaaaat gctgccagac accgtaaaac tggcctgctg ttatcggcag acgcagcccc
    18721 agttccgcca tcgtcagccg ctcaaatgcg gcttccagcg tttcgtcttt ctgcacgcgc
    18781 cctcccggca caaaccagta accctgcgcc gggcggttgg ttcttttgcc aagcagaaac
    18841 tcgccgcgac tgttctcgac aataaagtcg agagagacaa gcggtgtgga gcgcactaca
    18901 gtggcaaagt cttcctgacg taaaaacatc attccccccg aaagcggtct tgattctcaa
    18961 ggaaccactg gtaagtgctg gcaagccccg cttccagtga gatttcgtga taccagccaa
    19021 gctgatgcag gcgcgtcaca tccagcagtt tgcgcggcgt accatccggt ttgctggcat
    19081 caaaaaccac ccgacctttg taacccacca ctttggcgat ggtttgcgcc agctcgcgga
    19141 tagtgcagtc aacgcccgtg ccgacgttaa tgtgcgacag catcggttgg gtattctcca
    19201 gccagacttc atgcgccagc tccatgacat gaatgctcgc cgccgccata tcatcgacgt
    19261 gcaggaattc acgcatcggt gtaccgctgc cccataccac cacgtccgtg ccgtcgcctc
    19321 gtggaagcga cgcagcaatg ctggtatcac atgggagtta ctcgggtgga agttgtcatg
    19381 cggcccgtac aggttggtcg gcatgacagt gcggtaatcg cgtccatact ggcggttgta
    19441 agattcgcac agtttgatcc cggcgatttt ggcaatggcg taaggctcgt tggtcggctc
    19501 cagcgtgccc tgcaataact cgctttctgc catcggctgt tttgccagtt tcgggtagat
    19561 gcaggacgat ccgagaaaca tcagtttgtt cacgtcgttc tgatgcgcgg cgtgaatgat
    19621 gttgctctca atcatcatgt tctggtagat gaaatccgcc ggataggtgt tgttggcaac
    19681 aatgccgccc actttcgccg ccgccagata gacctggtca atgcgttcgc tggcaaagaa
    19741 atcatgcacg gcgcggctgt ccagcaggtt cagttcatcg cgggtacgta ataccagctc
    19801 cacatcaccg cgctgttcga gctgccgctt gatggcagaa ccgaccatcc cacaatggcc
    19861 agcgataaaa atgcgttgtt tactcatgct tatgactcca gcgcgatcgc cacgtcgtag
    19921 ccgtgagatt tcagcagaga gtgttttttc gccgcttcga ggtcattagc caccatttca
    19981 gacaccatct ctctgagggt gatttccggt ttccagccca gtttttcgtg cgctttggtc
    20041 gggtcgccga gcagcgtttc aacttctgcc ggacggaagt aacgcggatc aacggcgata
    20101 atcacatcac ccggtttaac gcccggcgcg tcatgcccgg tgacggaaac cacaatgccc
    20161 ttctcttcaa cacccgtgcc ttcaaagcgc agtttgatgc ccagctgtgc tgccgccatt
    20221 tccacaaact gacgtacgga gtactgaacg ccggtcgcga taacgaaatc ttccggctgt
    20281 tcctgttgca gcatcatcca ctgcattttt acgtagtctt tggcgtggcc ccagtcacgc
    20341 agggaatcca tattgccgag gtacaggcac gactccagcc cctgggcgat gttggcgatt
    20401 gcgcgggtga ttttgcgggt aacgaaggtt tcgccgcggc gcggggattc atggttgaag
    20461 agaattccgt tacaagcgta catgccgtag gattcgcggt agttaacggt gatccagtag
    20521 gcgtacagtt tggcgaccgc atacggagat cgcgggtaga acggcgtggt ttctttctgc
    20581 ggaatttcct gcaccagacc atacagttca gaggtggaag cctgatagaa acgagttttc
    20641 ttttccagac cgaggaagcg gatcgcctcc agcaggcgca gcgtacccat cgcgtcgacg
    20701 tcagcggtat attctggtga ctcaaaagag accgcaacgt ggctcattgc gcctaggttg
    20761 tacacttcat ccggctgtac ttcacgcaaa atgcgcgtca ggttagaggt atcactcagg
    20821 tcgccataat gcagatggaa tttcgggttg caggtgtgcg gatcctgata aatgtgatcc
    20881 acgcgctcgg tgttgaatga cgatgcgcga cgcttaatac catgcacctc gtaacctttt
    20941 tccagcagaa actctgccag gtaagaaccg tcttgtccgg ttacaccggt gatgagagcg
    21001 acttttgaca tgtcttattc ctctgtattt ttgaatttat tcagtttcaa cgcgttcgcg
    21061 tatcaccact gcgggattcc cccggcaaac cacatttgcc ggaagcgatt taaaaacact
    21121 gcttcgtgca cccacgacgg tgccgtcgcc gattgtgacg cctggggcaa caaagacatc
    21181 ggttgccagc cagcatttct cgccaatcac aataggcgtg gcgttaatgg tgaaatgttg
    21241 acttgcatgg tcgtggctac cggtgcataa ataacttttt tgcgatatca ccgaatgtgc
    21301 gccaatggtt atttcaccga gggtatataa attgacgtca tcgccaaccc acgcgtaatc
    21361 acctaaggtt aatttccacg gataggtaat ttttactgac ggacgaataa ctacgttttt
    21421 tcctattttt gctccgaata aacgtaataa aaaagcccgc cagcgataca atacttgtgg
    21481 cgaccaggca aatattgttg cctgtactgc ccaccataat tgcactttaa tagcgttgcc
    21541 gccccggaac cctttcggca ccgagaaacc gcttaaatct tgcattggtt ttccttatat
    21601 tcagactttg ttatataagg ctttagtctt acctgtcgta cgttggcgta aatgccagga
    21661 taattctgcc cagaagccag gtacatgtaa tatctgccgt tggacttttt tggcgtcagc
    21721 ac
//
LOCUS       DAEBXD010000062        21703 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEBXD010000062 DAEBXD010000000
VERSION     DAEBXD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21703
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.062"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            60..1061
                     /locus_tag="KJL94_002535"
     CDS             60..1061
                     /locus_tag="KJL94_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD4410841.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1352..1786)
                     /locus_tag="KJL94_002536"
     CDS             complement(1352..1786)
                     /locus_tag="KJL94_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD4410842.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1758..2549)
                     /locus_tag="KJL94_002537"
     CDS             complement(1758..2549)
                     /locus_tag="KJL94_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4410843.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2549..3229)
                     /locus_tag="KJL94_002538"
     CDS             complement(2549..3229)
                     /locus_tag="KJL94_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBD4410844.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3226..4425)
                     /locus_tag="KJL94_002539"
     CDS             complement(3226..4425)
                     /locus_tag="KJL94_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBD4410845.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4426..4779)
                     /locus_tag="KJL94_002540"
     CDS             complement(4426..4779)
                     /locus_tag="KJL94_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410846.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4779..5519)
                     /locus_tag="KJL94_002541"
     CDS             complement(4779..5519)
                     /locus_tag="KJL94_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBD4410847.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5742..6032
                     /locus_tag="KJL94_002542"
     CDS             5742..6032
                     /locus_tag="KJL94_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBD4410848.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6056..6400)
                     /locus_tag="KJL94_002543"
     CDS             complement(6056..6400)
                     /locus_tag="KJL94_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410849.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6413..7477)
                     /locus_tag="KJL94_002544"
     CDS             complement(6413..7477)
                     /locus_tag="KJL94_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410850.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7480..7785)
                     /locus_tag="KJL94_002545"
     CDS             complement(7480..7785)
                     /locus_tag="KJL94_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410851.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(7787..8389)
                     /locus_tag="KJL94_002546"
     CDS             complement(7787..8389)
                     /locus_tag="KJL94_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410852.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8389..10404)
                     /locus_tag="KJL94_002547"
     CDS             complement(8389..10404)
                     /locus_tag="KJL94_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBD4410853.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10582..11007)
                     /locus_tag="KJL94_002548"
     CDS             complement(10582..11007)
                     /locus_tag="KJL94_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410854.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11011..11451)
                     /locus_tag="KJL94_002549"
     CDS             complement(11011..11451)
                     /locus_tag="KJL94_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HBD4410855.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11462..12607)
                     /locus_tag="KJL94_002550"
     CDS             complement(11462..12607)
                     /locus_tag="KJL94_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HBD4410856.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12611..13168)
                     /locus_tag="KJL94_002551"
     CDS             complement(12611..13168)
                     /locus_tag="KJL94_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410857.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13143..13532)
                     /locus_tag="KJL94_002552"
     CDS             complement(13143..13532)
                     /locus_tag="KJL94_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HBD4410858.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13519..14073)
                     /locus_tag="KJL94_002553"
     CDS             complement(13519..14073)
                     /locus_tag="KJL94_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410859.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14070..14477)
                     /locus_tag="KJL94_002554"
     CDS             complement(14070..14477)
                     /locus_tag="KJL94_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HBD4410860.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14476..14710
                     /locus_tag="KJL94_002555"
                     /pseudo
     CDS             14476..14710
                     /locus_tag="KJL94_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14707..15648)
                     /locus_tag="KJL94_002556"
     CDS             complement(14707..15648)
                     /locus_tag="KJL94_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBD4410861.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(15660..16163)
                     /locus_tag="KJL94_002557"
     CDS             complement(15660..16163)
                     /locus_tag="KJL94_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410862.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(16168..17388)
                     /locus_tag="KJL94_002558"
     CDS             complement(16168..17388)
                     /locus_tag="KJL94_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD4410863.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(17403..18140)
                     /locus_tag="KJL94_002559"
     CDS             complement(17403..18140)
                     /locus_tag="KJL94_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD4410864.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(18028..19494)
                     /locus_tag="KJL94_002560"
     CDS             complement(18028..19494)
                     /locus_tag="KJL94_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBD4410865.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(19494..21116)
                     /locus_tag="KJL94_002561"
     CDS             complement(19494..21116)
                     /locus_tag="KJL94_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HBD4410866.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(21119..21691)
                     /locus_tag="KJL94_002562"
     CDS             complement(21119..21691)
                     /locus_tag="KJL94_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBD4410867.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
BASE COUNT         4928 a         5929 c         5385 g         5461 t
ORIGIN      
        1 atggactatg acataaagat ctgatatttt cctcacaaaa acttagggat tttattttaa
       61 tgcataagag caactgcttt gatacggcaa ggcttgtggc agctatgatg gttctaatca
      121 gtcaccacta cgcgctctct ggacaactcg agccgtatat attcggcttt gaaagtgctg
      181 gcggcattgc tgttatcata ttcttttcca tatcaggata cctgatttca aaatcagcaa
      241 tcagaagtga ttcctttatt gatttcatgg ctaaaagagc aagaagaata ttccctgctc
      301 ttgtcccttg ctctgtactg acgtattttt tatttgggtg gatgcttaac aacttcagta
      361 cagaatattt tagtcatgac attatccgga agaccataag ctctatttta atgagtcagg
      421 ctccagatgc cgacattaca tcacatttaa ttcatcccgg tattaacggc agcttgtgga
      481 cactaccact ggagtttatg tgttacataa taactggagt ggctgttgct ctactaaaga
      541 gcggaaaaac gttcatagtt atactattga tttttgtttc actttcatta gttgaaagtg
      601 tatctgagaa caaggatgta atattctcca ttccagtatg gctgtatcct ttgcggggga
      661 tggctttctt ttttggtgcc actatggcga tgtatgagaa atcttggaat gtaagtaatg
      721 tgaaaatttc cgtgatttct ttgcttgcta tgtacgcata cgcgagctat ggcaaaggca
      781 ttgactatac aatggcttgc tatattctgg tttctttctc aacaatagct atatgcacat
      841 cctttggaga tccactggtt aagggtaggt ttgattactc ttatggtgta tacatttatg
      901 cattccccgt tcagcaggtt attataaata cattgcatat tggcttttac ccttcgatgt
      961 tgttaagtgc cattgtcgtt cttttcttgg ctcatctttc ttggaatcta attgaaaaaa
     1021 gattccttac acgaagctcg ccaaaattat ccctgaattg aataataaag gggccatcga
     1081 gcccctgaat aaatcaaagc cagatataac actgcctggc agtggacacc gttctgatgc
     1141 ctcgtcctgc aaggacgagc gaaggtggag ttcttttcca cgcagctatt tagtacagta
     1201 ctgagtcata ttgtttatgt atgagttaac ttccgtaata tcaattaaat aaaataagtt
     1261 gaggccattg tagaagtgag cattatatcc aggttatctg gacctgttga aaaatgcgta
     1321 ttattccaac aattacccta gattaacaag tctatttatt tggatgcata ggccactcgg
     1381 gttttactac attaacacgc ataagcagta cgcgatattt tttccatgcc agtaacagtt
     1441 tcgtttcatc atctgttgcc atacctaaat caaccgcatc ctgaagcggc gctattacat
     1501 tcgttgcctc tgctatcagt gcagatttct tttcatcatt tattaaggct aattgttctg
     1561 gtgttggaga aggacggtca acaatgacag ggtgaccgtt ctcaccacag gtaatcattt
     1621 ttgttacact ctgtgagtta atcagtgttt tccactctga ttccgagatt tccactgcat
     1681 catcaggaat attggtgccg tgaaattcag tcgaataaaa tccgtttgtc gatgcagaat
     1741 aaaaatatga catttcgtta atatcccatt gcaatgtaat acgcatcagc agatgaacca
     1801 tatcctgaac cgggcgacac tgtaatatca aatccagtgt tatggacatt tgtcgccgat
     1861 acagtcggct gccctgacgc gccagtaacg ttcaatgtaa ctgtgacagc aaggactctt
     1921 gagaatgtca tcgggaacct catcccgtat gttgaagata cattcgggga gccattcaac
     1981 tgaccgcact gtaaaattaa tccagacgga aatttttgcg cagcagccgt tgcggtatta
     2041 cttgacgcga acaggctcat atcaggtatc tgattcgccc ctgtacctac attcctttta
     2101 gccgcttctc ccaaaccaag gtatgtgaga atgtcagcaa tagtattttt cccaataatg
     2161 tcacggccaa cagaagttaa atcagtctgc gctgctgtat catttccagt gaaatatggg
     2221 agtttatttg cacctgttgc gagcccagct aatgccgaca gcgtggcatc aagcgcctgg
     2281 aaatctttcc cgaaagcggt ccccattttg gatataaacc cgttcaggtc tccatcatca
     2341 agcacatcca gcctgctttt gttggcggtg tactgcgcca acgctgccgc gataaagctg
     2401 gcttgccgaa tagctttgtt gacctgtgcg ctggatgctt tacctgccgt aaaccctgaa
     2461 agcagagccg gaagtgcttc ccagtcagct tgtgaggtga cgttagcgtt cggatcaagc
     2521 gcgaaaggtt taaagttgtt tattgccatt agagtattgt cccccatgct ccaacatcga
     2581 acccgccgat gtattcgtta tccatatcaa acccaaagaa tttagagccc tcggatggtg
     2641 cttctaccga aggcgtttca acatcaccgg cccatacgcc agctgcttta acggtgagat
     2701 agccctgttt gatagcggca atcagttcaa gagacacatc agaaatatca gtctcgggga
     2761 atacccaaac cgatatcgtc atgtcctggt tgtcgacaat ttgcatcctc aggcctgagc
     2821 cttcagtcgc agcgtcaagg atgggaggca gagagtcgtt ccgaccgtcc cagttgttga
     2881 tagcgatttt cgctttcaga atgatgcggt acgtctcatc gcttagcgtc gtatagccag
     2941 aatcaggatc atatggccct ttccagatgc cctggtcata cccaagcccg tcagtgtccc
     3001 agctgaaata aactccgcta attggctggc tgactatgcg actgcgtccg atccacagac
     3061 cgaggatgtc gagctgtaca ccgacagcag tatcaatatc gaaggctgtt ataagccctg
     3121 acatagtgct ggacacatca atcagcgggc gggtgctcag atctatatgg tcaaaaaaga
     3181 gtggcttggt agcgtggtag ttagtgatca gttcggtgta tttgctcatg aggtcaccgt
     3241 gatactgata ttcgcggtgc tacaggacgc agaagcatca taggcaatat caatgtttga
     3301 tgccgatacg ctgccagacg acttaccgat cagcaggtcg gtaatatcgt aatagcgggc
     3361 attcccgccg ctcacaacgc cgaggtttgc cggggaataa atacggctca gcagaacgtc
     3421 gtcgccaatt gttaggccat ttatataatc ggcaacagcc tgtttaatct gctcgccgat
     3481 ttgagaggta tagccggtaa aaactttcag ggtaatggct acgaaaattg gcacatcggt
     3541 agagcgcgaa aaactgatga cgtgtggatt accgtaagta tccggcaccg tgacagaagt
     3601 tttaccgtaa gttgcggttc cctgcccttt actcccccgg attgtttggg ctatctcggt
     3661 aacatcccct ccatcgacga tggcggaaat agagtgtggc ggcagcccgt tgctgtcggt
     3721 tgccccagtg tcgttctcat atagtttgtg acgtgtcacg ccagcaacat tagcgatagc
     3781 accgtcgaca ccttcaaacg gtgtgatcga tggtagcgcg acgctttgcc cctgccgaat
     3841 gcgcagctct gcgtcggttt cggctggtga accgacagta gccgcaactg gattggttac
     3901 cgacacccaa cctcgggtcg gggtgttgat agtggtaata gtcccggcca gcgccgcaac
     3961 cgaaccgcta ttcgcacatg tggccgtcac cagcacagta ccatcaacgc cgatcgctac
     4021 acttgcggga aaattccaga taatgccgtt tttatcccgt gcggagccat tcgtgatagt
     4081 cgtgcctgcc gtaccggtta acagaaggtc agcagtagag tttgtcgcta cttttcgcgt
     4141 gatcccgtta attttcacat tgctgctaag cgctgcggcc tgcgctgtcg tcggtgaaaa
     4201 agagttgtag atcccgatag cggtgttgtt agcgtcatgc acggcaagag ccaccagcgc
     4261 gaccatctgc ccgtctttgc tgtctggttc gaggtaggca tcactaccgt aaatctgcct
     4321 gaaatagctg gtcagtgtat ctaggattgt ctggtaatca ggcgcactaa tcccctgggc
     4381 ggttaccgtt gccgatagcc ccagcgtgtc gaggttcaaa gccatttatg cctcgcttgt
     4441 tacagtcgtc tggccgtaga ttgtgtcaat ggaggaagtg aaggtgacgc gacggctggt
     4501 gccgtcataa ttggtatcga aggaaagaat cgacagaacg cccggtgtat cctgtatgcg
     4561 ttcgcgtata gccaggatgt agacatctga tcgctgcttc ccaagcactg actgaacata
     4621 cggcgtgcct tccgtcagat cgagaaacca ctgaccgcgc cacagctcga aacgggtttt
     4681 tacggcctgg gcgacacatt ccggactgtc gataaggaaa gtatcgtcac cctggccgaa
     4741 agtgtaatcg ccttcagtat cttcgcgacg gtatcgcatt attgcggccc tccggtagtt
     4801 cccccgccag tctgtactcc gccatgttta tgcgtggcga cactgatacc agaagctgtt
     4861 acgtcattcg ttaccatcac cggcccaagc atcgtcgcag taccgccgct ttcgcccatt
     4921 ccctgagaca agttgccatt aatcgttacg ttgccgttca gcgtgatagt cggggatgtg
     4981 attgtcgttc caccttcagc cgtagctgta agctggcccg gtgttttaat ggtgatgtta
     5041 tgtcctgcgg cgacttctac aaacgccgca ccatcatcgg ttcgcagctg cgcggcgctg
     5101 gtactgatac cgctgatttt ccgtgcttgc gactgcgggc caacgatggc gaacgcatca
     5161 gataagtcat gctggcgcgg gtcgacggtc tcctgaacgc cgccgctctg ccaccaaaaa
     5221 tcgatgcaac ggtcagcaaa aatcaggagg cactcgtcgc cttcttttac cggaaaggtc
     5281 agcgtgcaac cgccgccgcg cgggaagatg accggcacat ccaccagcgg ttttaattcg
     5341 gtggatccat cgccaacaat accgcgtagc gccacctcta ctgtgcaggt aacagtctca
     5401 ggatcgaacg actgaatgat gccgggcatc gctacgcgca tctgggtaga caccgaatcg
     5461 gcaatggcct gcgcggtctg ctgctcaccg ccgatctgtg attgagttgg aattggcata
     5521 aaacccccat aaaaaaacca gccgaagctg gtttggtatc aattatctct tagtaactaa
     5581 gctacatcag tgtgaagtaa ggcttttccg ctgcaatggc acacattcac aaggtgtatt
     5641 aggatatgtt tgcaacaagt aagcattgaa aatcaatttc agccatgaag ccgccataga
     5701 gtggacctgc accatcgcat tattgactac aggagtaaca aatgggattt agatttcgta
     5761 aaagaatccg gattgcgccc ggactcgcga tcaacattag taaaagcgga gtaagcactt
     5821 caattggtgg taaaggttcc accattaaca tcgggaaaaa aggcgtaaag atgacaaatg
     5881 gccttcccgg cacggggctg tcgcatacca ctaaccttta ctctcccggg aaatcgacag
     5941 aaaaaaaaca acttactcat aagcaaaaaa taattagaaa catcctgttc gtaattattg
     6001 tgtttataat aattaaagct caatattttt gacgcatgcc cgcctatctg gcgggctatt
     6061 ttacttttcg gcagtcgtat gttgcatact gacgcggcga atccatgctg gcttgcagcc
     6121 actgtgcgtt gagaatggct ttgccgtttc gattgatgta ctcaagacca acccatcttc
     6181 caggctgatc ggtagccata cgccaatcca tcttgatatt gttataatcg cctttgtttt
     6241 tcaggaaggt gattttttgc atttctggct ttgcgccatt gattctggct aagccatcat
     6301 ctgcccaatg gattttaaaa tcaccacatt gctgatccgc aaaagcgggt gctgatataa
     6361 gcaccgcgaa cacggtaaca gtgcaaagta aatgtctcac ggccccacct cactaattcg
     6421 tcgtcccttg cattgctttc gggctataaa gatcacgagc gccacgcgca aaacacatca
     6481 aatccatgta ccacgcctga cctctggtgt cgccagtata gtcgatagct ttgacgatat
     6541 aaacgccatc cgtcgcaatg ctggcagcct gtgacgtcgt gccggtcagc acacggttgc
     6601 cgttctcttc tgtttcggtg atacgcccgg gcgactgtgc tatttcgcta ttgccgagcg
     6661 tggcgcggta caccgaagcc tgatcgagct ggataagacc attaatacgg atgtttgggt
     6721 ttatcaggca tcgcacgttt actccgccac ccatcgtttg ttgcggcata ccgatcaggc
     6781 cagtattggc attcaacaca atggcttcgt gaatatattt atcctccggc accatctgga
     6841 cctgaccatc caccagttgc catgtcgctt tgcactgcgc agcaatatta tccatcacgt
     6901 tgcggctgga tgagtaaatc gcgcggccac gaggaaacac ggtatcagga aaatcgccgg
     6961 taatgccctg tgtcacgccg aacgcgttga aatcctgcat cgtcgcccgg tgcagatccg
     7021 caacggtata tccagcggca agcgtggtga tggtggtcgc atagaggaac gcttcgtgat
     7081 caccaatggc ctgaatcaac acccaggaat ccgtaatgtt gtccttcccg gtgacggtga
     7141 agcgaatatc accgtcaaaa atcaggccgt agttctgacc gttcacctgc cctatctggt
     7201 ctggtgaaat ctcccgggac acaccaacct ggctcgcatc aacatccggc gctataccgt
     7261 catacccggc aatgatgcga attttggcaa actcctgccc aagtatcttg ttcgtggtat
     7321 cggtcgaaag gttgtaaatt ttcacgttcg ccacgcgcgg ccagcgtgtg tctgcccact
     7381 cgatctggaa cgtgacctta aagtcagaca gggaaacgcc ctgcccgttc tggtccaaca
     7441 gttgcagctc aaaatggcgc atccagttaa gagacatttc tactcctgta cgaaaatgag
     7501 gtggctgtat gtgccgaggt tggttttggt gggctcgtct ggtgcgccct tatcggtcgc
     7561 caccaccagc gcgccatcaa tgccaagctg tggatattgt cgtaaaaggt ttacgccggt
     7621 cagtagaggt acgccagaga gaagcgcggc accgccgcta tccatcacgt ccataatcca
     7681 gccagccgca tcacgccaga tgatcctgag tgtatacgtg gtattgccca gcaaaactcg
     7741 gaactgctga ttgtcaggag aaagcggtat ttcgttaaac tgcatatcat cccccgaatg
     7801 ctgatgtaac gcttccgccc agctggctca gcaaggattc gtttggcggt gtagtggatt
     7861 tcatcccgga attctgcacc gccgatgtgc tgacgccatc ctgcatatct gatttatctg
     7921 caacgctaac gctctgcgtc tgcgacatga tcacttcacg cagggtaagc gtgcagttca
     7981 gcacgttctc gctggtttta tccgttgtca cctcgatggc tcgcaccagc atattgctgt
     8041 aaacccgctt tccggtcact acatcgaacg gcaccctaga ggactggaga tccagtagtt
     8101 gctgataggt ctctttcggg ctaagtccgg cgctgaggcc gattgacgat gtatcaatga
     8161 agtccagcaa cgaaccgcca cctgcgaagc cgcattccat cgtgacttcg ctggggcgct
     8221 tatacgcatg atcggcgatg aaacccgacg cgctattcgt tgttggcttc tccaccgggt
     8281 gctcagtaat ttcgagcgca tcagaatgct tttcagaaac aaccacgctg gggattaata
     8341 ttccaattcg ccgggattgc tggcgaaaaa ttgctgataa aatatccatt atctcggtcc
     8401 tgcggggagt tgctgggtta actgtgaatt cacgcccttt tgacggtcaa cagtcaaacg
     8461 ggcagcctcg cgcggatcga aaacgccgtg gatgttaata ttcgtttcct gctgaatcac
     8521 cggggcgctg gtgggcatat tgctcattac tttcggaatg tagttgcgcg tttcctgcgg
     8581 cattagcccc attccataac gcttaacatt cccgatcccc cagttatatg atgccagtgc
     8641 tttgctaagg tctccgccgt tctgccgcaa cagctggctc aggtacttag cggctgcctg
     8701 agctgacttt tccgggtcga atacatcatt cccacgcagg cccatgtcgc gcgccgtgcc
     8761 atccataaac tgaaacagac ctttagcgcc agcgcctgaa acggcgaact gattaccgcc
     8821 cgattccgtt atggccacgc tgcgcaataa cccctccgga agccggtaga gctgttccag
     8881 gttggtaaac atcggctgca tccatcccag cagttcagag cctgctttgg ttggctgtgg
     8941 ccgcttaact gactggccga gctgctcagg ctcatcatca ccgaaccagc cgcgcaccgt
     9001 tcggcccacg ctgcgaggat cgaatcccca gtgctcttta atccagtcgg cggttccgtt
     9061 ggcgctgtct gttaccatcg gcatcgctga cggattttcg ctgccctgat taagcatctg
     9121 tttgccgatg ctggcggcat cagcccagcg gccatcttta atggcgttga gcaggtcggc
     9181 gatcatgttc agcatcttgc cgaactcacc catttgcgaa atgaagttgc taaaatccca
     9241 ctttagggac catgacttgg ggtcaatacc gagcaactta gccagcgctt ttgtcaggtc
     9301 cgtaacagtc tttttcaggt caccaatcat tttgacggcc tgatcaattt cagtcttcca
     9361 tttcccccag tcaatcaggc ttttgccacc ttctttccac gtctggtaat catccaagag
     9421 cagaccaagt gcaataataa gcgtcgttat catgcccaag ggtgatgaca tgaaagcggt
     9481 atttagcagc cgccatgcaa ccaacagccc actgaacaat gcgataagct gctgcgtggc
     9541 tgggttcagc tttttgaacc aggcgatcac accctcaaca gcttgcccag ttcgccataa
     9601 cacgcgggta atcgcgtcac ctgcccagag aattcctttg atgatcttcg tgatgaccgc
     9661 ttcaatcttc ggccagttgt cgagtatctg cctgcggaag ttatcaatac tgccagcaag
     9721 gccaccagct aggttagagc caatcttgtc tttcgcctgt ccaagcgtca tcgtcagatt
     9781 acgcatggag gtcatgaaaa tattggactg tttagccgct gactcagcat taaaaccaat
     9841 acgctttgcc gtcaacgcgt actcagaact gagctgcccc atccctctgc gcatcgccat
     9901 cagcgtgttt tcatcgatgc cgagcatctg cgcgtattgc ttcgcgcggt aatacggcat
     9961 gttgttgagc ttttggccaa cgccagtaaa gatggcagca gtatcacgca tctttccgct
    10021 ggcatcgcgg gtctggacac caagacggtt caggaaccct tccgcccccg gattgctacg
    10081 catgaagcca gccaaccctt cgagggagga catagccgac tcggcgctgg caccggtttg
    10141 cgatgcggcg tatcccagcg ctttgatgcc ctggacactg gcccccgtcc gctgggatgc
    10201 ccagtaaatt ttatccagac cgttcgcgat ctgagtggta aatccgacaa tgctcagcgc
    10261 tgcgcctttc accaccgcgc cgaccttcag aacgttcgcg gtaacgcctt tcagcacggc
    10321 ttcaaactta ttagcgccag cctgatcgat atcgaatccc agcgaaacaa ggaaatcttt
    10381 aatcgtatct gcgttaccgc tcattggccg ctctccattt atctacccgg gcgtcgttat
    10441 cctcgcgcat gtcgaggtag tcattgagaa gcgcgatgcg gcaaagatct accgcaccgc
    10501 tgttaaggtc tttctggtca atatggaagg caagcgccgg gcgaagaata aaatcttcac
    10561 cgcccggcag gctgttgaag gttattccgc tagcggggtg ggcgtctcgc tggtagggag
    10621 tccttgcaaa aaatttccca gcgagtcggc gaccacccgc gccaccagtt gcagcatggt
    10681 aagcaggtcg atatcgtcaa acgccatttc gccatgctgg cagaccggca cccagccttt
    10741 catgtgctcg cgtgaaacaa cggaaagaca ggggaacagg atagcgtcca cgtcgccatc
    10801 actcagatcg gacacagcat tggcaatctt tggcaggata gtagccatcg cgccttcggt
    10861 gtctttgctg ctgatcttct cctgaacgct ccggaagtcc gaaaccatcc cggccagaac
    10921 cggcaacagc ttgcgggaca ccttcagctg ttcgaaaacg ctgagctttg cggtgcgata
    10981 tttcacgcct ttaatttcga attccatgcg ttaaaactcc ccgagaagct ggtcaatctt
    11041 gccgcagtcg aatacccagg cgacggttcc gccctcttta gcgttattga aatcaggctg
    11101 tttctggaat gcacacgaac gcgcagtaga aatatcaccc gatgccgtgt tgcgaatgac
    11161 gatcacgtta ttgccccagg tggcagagga ctggctttgc gcgttatacg ccagagacag
    11221 ctttttattc accggggagg ttttgagtag cgtcaccgta atggtgcctg acttatcggc
    11281 gtgcaggctg tgcatcacct cgccatcggc accgatagtc atggtgtttt tgttgccgcc
    11341 catggtctgg gtgatacctt cctcagagtt cgcagaaccc tgaccaagat cgataacgcc
    11401 ggtcggcccg gtgagcgacg cggttacatc gagaaaagaa taagttgcca tttatcgctc
    11461 cttagcgaac cacgttgatc tgcacatcgg cataatgaac tgcgccagcc agcttacagg
    11521 ccacctggat taacggtgct ttgcgcgctt cacggtcggc ctgcgcctgt tcggacagcg
    11581 gttgcgcata cacgtaatag cctttggtca gcgtatcgcc ggaattcagc tgtccgatag
    11641 ggccaccatt ccacacgcca gcagctacca gaccgttcgt gacggactga tccatggact
    11701 gttcaacgtt ggaaagcaga cgggtcacac cggcatcagt ctgcggaatt ttggtggtgc
    11761 tggtgtaaag caggttatag aggttggtct gaacgtagtt ctgcaaccag tcgagcccgt
    11821 ggcgctcgtc gaagaagtca ccgttcgcca tgacaccctg ttgcaggatc gccgtgtcgt
    11881 tggcgtagta cacgaataca ttgcagtttt ttgcatcaag tgccgatgcc tggctgactg
    11941 tcagtgtttc atacccgaca cccggctcct gcttaaactt gagcgtaatc gcggtattac
    12001 tgccattgaa attaaccgtg aatgcccggc caaatgcaga taacgcagcg tatttattac
    12061 ccgatgaata ttgaataaaa ctgcgtgaat atccggcggt tttcagtttt gatgccaaat
    12121 catcgctgga tgcagtctgc aggcatttct catcgcttgt cgtaatcgcc agaatacggc
    12181 ttacagaaga ggattcgatc gccgcagcca ctttcagcca gtctgcatcc ggaatatctt
    12241 catcgtctgc aatccccagc ccataccatg aagtataatc gagcatggca ttcacagcct
    12301 gctccagcgt ctcaggcgtg gcctgttcgc tgtctccctt cgttttcacc caacgaccaa
    12361 caaaaacctc ctgaggtttc ggtgattgtg agaaaaacac ctgcgcagcc ttatattctg
    12421 gtgattccac gccaaaatct tttccaatat cttccgcggc agaataacgg cgaatgcgct
    12481 cacttaccgg aatgattgtg gacgggccga gaatgagtaa tgcaccaaaa tttcgccctg
    12541 atgctgcacg cggcgacatg atcacatcaa cattaacaac gtttgataca ggcaagccct
    12601 gtgccatagc ttaatctccg aaaaagatga ctggtgcttc caccagcgat ttaataccgt
    12661 actcgcgcac aaccttccgg cgcagacgca ccgtcatatc gtagcggcgg acccattgct
    12721 gattaataag ttcagggaag agacctgtgt aatcgccaag agacagcccc agcgcattca
    12781 gtgctgcgtt gttctgcggt acagatatac cgtcacgaaa ccgggacgca tacaccatcc
    12841 ccgccggacc ataaaacgaa gccatacact caatcgtttc atgccgccag agctgagagc
    12901 catcatcggt ctgtctggtg aatgccggac tgtcatcacc tgaccatccg ataaccccaa
    12961 acgcacacca gttcgtttca accggtagca gtggcggctg ctctttctgc cagcgcgggc
    13021 gaaccatccc ggcagacaga ccggaaacgt tacgcatcca ctggcttaac agcctgtcga
    13081 gcgcttcgtc ataatccgga tcgccactgg ttggtattaa ccatccgcgc tctgtactgg
    13141 tgttattgct caaccggagt tcccccatca aacggcatca actcacaatg cgcctgaacg
    13201 aatccggccc cataagctgt atacgggtcg acgaaggtca cacgataatc acggccctga
    13261 tacgtcacga tatcggcatc acggccagtc tgtccctgcg tcagtcgctc agtcgtcaca
    13321 atcagaattg caccgctgat tacctgccct gcctgcatac ggcggttttc cagagagcga
    13381 tcaacagtta cgactccggc aaactgcttt ttaacttcac tgtcgctgcc gatcccgtcc
    13441 tcatccaccg tttgcactcg gcgtgttacc cacaaattga agtcgcaaaa atcggggtca
    13501 aaaagcacat ctgttacatc aagagtcggc atctttatcc ctcacaacat gggtaatagc
    13561 tctgcgatat tgcccggtgt caattaatgg tttcgccaga tcggttccgg gagattcgcc
    13621 agcaacacgc cgagcaagtt ccagtgttgc ccccttgcgc ccccgacgag cccgggcttc
    13681 aacggtgcta tcagcaagcg gtgtaaagcc ggtaatggtc atgtaacgcc tgacgccatt
    13741 cgcggccagc gttccggcgc ggttaagcgc tctttccgca cccgccgcat ttccatcaag
    13801 cgcagcctgc gccgctgctt tgagctgcgg caccgtctgt tcctctacgg atttaacgcc
    13861 ggggatcagg tgcgggcgtg ggggtatgtt ttgcgctggt gagccgtatt cgttgacgta
    13921 accgatcccg gcattaccaa acggaacatc ctcacgctcg ctgtcttctt ccgggatgcc
    13981 caccagcaca tcctttttgg ttagcgaccg gagcgcatcc agaatggcct gagcgttatc
    14041 aaccctcgtt gttacaccac ttttgaaact catagctggc gaccgcccgc accgaacatc
    14101 gtgatcagct gataaaattc agcgccatat cgggtgttat tccagaagcc tgcgtcaggg
    14161 tttagcgtcg cgctggtgtc atagctgacg cttaccttgt caacggactt ggaggactga
    14221 acaccattgg ttgaaccgcc cgggccgcca accagcattg cccgactatc tgccgcccat
    14281 agcgtcatgt agtgagccac gaacaacccg gcaaagtacg gaaacaaatc tttgccggtg
    14341 acgttttcgc tcagcagttc atcggccaga ttcagacgaa actggatttg cgcttcggga
    14401 tatttggcag ggtcagcaaa ctgcgggaag tcgcggcgaa aatcacttac cgctggcagg
    14461 ctttgattct ttggcatttt ttacctcgtt acgcgcgtct gtggctttgc caacggatac
    14521 ttccgcgtgc gcacgagtga accagtgcgt ggcaacgtct tcctccactg catgacggcc
    14581 tttaacaaac tcgcgccgcg aaccgtcggg aagcgtgagc acaaacgggg tatgtacgtg
    14641 tattactgca ttattttttg ccatcgggtc atccttaatg gccccgccag gggggccatg
    14701 tggctgttaa atgccatcaa cgtacgaaat ggtttctttg tacactggct caaccgcacc
    14761 cagcttgccg tagtaggtcg caatctgata cagaccgcga tactggatag gaacgctctg
    14821 caacggcacc agcggatagc ggacgtattt tttatcgttg gtgtacgcaa ccatgcgatc
    14881 cttattcccc acaccacggc ctttcagcca tttaaccgcg cggatattca gcggaacacc
    14941 gttctggtga tagctgatgg tgttggtctg aaggtacgtc aacagggact ggttacccgc
    15001 agatgaaacg atgatgctgg acaacagagc aaactgctct ggcgggatca gcaaatcacg
    15061 cggaaccaca gagtaaccgg aagcggccca cgcatcagac agcacctggt taatgcttgc
    15121 gcggatttcg tccggtgttg agattgccca cgttttggca gcgttgttga caggcacgcc
    15181 gtccagggta acaaggcctt tcaggtttaa tgcggaatcg ccaacataca cctgctcgtc
    15241 gttgtccatc tgccatttga gctgcatccc gtcgtacttc tgggtgtcaa tcgggcgacc
    15301 tacctgctga gctgctgcca gctctacaac ggtccagccc agttccatgc cccagagatt
    15361 cagcgggtta ccggatttgc cgatatccac gtttacgcca gcaatagcgg ttgagtcttt
    15421 gcctacccag tttttgccat tcggatttgc accagtaccc gcagcggcga agctggtatt
    15481 cgtccagctg gaaatgtcat ctgcgataga gacatcttca cgcaactgaa tatcgcgggt
    15541 ccaggtgtac cccgccagtg gcaggttcag agtctggtcg agtcgctcca gctctccgat
    15601 gagaaaggca ccagagctgt caacggttgc ctgatcaaaa gtaatcattc gtctgttcct
    15661 taaatcttcc aggaaatttc tgcattgccg ttagcatcac cggcacctgt gaattcagcg
    15721 ttggtcagca ccacattttt gccactgact gacgtggaca tgaatccacc cagcggcact
    15781 ttgatggatt catcagtgga gacgacaacg tataccgggt cgcctttttt gatggtgctg
    15841 gcatcaaaat cagaaccgag attaacggtc acgtagccac gcttcatggc gtcgcccggg
    15901 aagttcttgc ctgttcccac ctggcgaacc atgtccggct gcgaagtggt cggataaggg
    15961 cgcacgtaga tccccttcac cttgtctgcg gtatcgccat ctgccagcgg cacgaaaaaa
    16021 ccgtcagcat cgtatttacc agccagccca taggcagcaa aggcgttatc ggatttaagg
    16081 accaccggtt cgacggttaa gtcctgcggg cgagagatag ccccggcaat gccaacaggc
    16141 atccggtaca gatatgcagt cattggatta tcctttgcgg ttagaccaga attcagcgtt
    16201 ttgtttgttc agggaagcga tgctggtcat gcccatgttt ggacgttgtg catcgcccgt
    16261 ggtgctgcgg gtgtttcgcc ctttggcaat ctcagacaca gcgttaaacg ccatatcgac
    16321 cgattgctta ggcaatttgc ggatatccgc atcaccgaca acctggcgaa ccagcgtttt
    16381 gtcagcagaa gccagaacct cgcgtttgaa cacggtcggt ttcatcttac ggctcagatc
    16441 gatacccgga acgataactt cggcacgcca ggctgagtca ccagtaatcg tggtttcctc
    16501 ttcatcgtcc tcgccgtcac cggtcggatt atcgtcaggt ttattatcgt tatcgcccgt
    16561 ggcatttcct tccagcttag ccagcagggc tttcagtaat gttttgaggt catcatcact
    16621 gtcgccggtt ggacctccgc ccatctctgg tactttgtcc ggtagcggtt gctgcgggga
    16681 caggttgatg ttgagattaa cgccctgcgg caaatccccc tcatctcctg taaccgatgc
    16741 gggagccgac tccaccagtt cgttcatggt gtcagcgtca cccgttttga tggccgtgcg
    16801 catgcgggtc caccagcttt tcttttgatt tgccattgtg tctctgtctc caattgcaca
    16861 acgatttccg gctctgcctt tggggacaag agccacatgg tttccggtaa tatcgacctg
    16921 ctcggcttta cctggctcgg tctgctcgta ctccgcgtca tagccgcacg acacttcacg
    16981 caggccatct tcgataagct gaatggcgtt ttcgtctttg acgataaggt cagccagcat
    17041 caaatcagag tggtcacccg tcccgcgccg cacattctga agatgcccga cagcaagctc
    17101 tttccagttc tcgggattta ccagccgcac attcccgttt tcatcttcag gatgcaggat
    17161 cgtgatgctc atcccttcga atgaggcgag cgtggccgga tggaatacct gctcaggaga
    17221 gcgcgttacg actatctcac cgagcttgtc gggtttgagg tttggcagat cggcagcgcc
    17281 gtagagctgt ttacccgttc gacctatcgg cacgtctttg cacagcagcg agccgtcagc
    17341 cagctgatag cgggtttccc ccagccgggt attgaaaaaa tatttcatgg tttacctgcg
    17401 attcaggcga gataagaatg agggttggga aaaacgattt ctttataaca gcgacaattc
    17461 gggagctcgc cagcgtgacc ggtcatgcca tcaagcgttg gaggtttgcc ccattcgaca
    17521 aacttacctt ccatctcccg atgagaatgc cggacgtcgc catcttcggc tgtacgccag
    17581 atataaccat tcgagccgat tgacagcgca cgcgcctgat cgagcgcgcc ggttgcacgt
    17641 ccaagctcgg tacgggcgat aaggttcgct cgtgagcgtg acacgtcacc ggacgctgct
    17701 atctctttcg cgaatggttc agcgcggcca ccagccacaa cggcctcgat ggccttgttc
    17761 tgaatgtcat acacccgatc ggcggcctca agaggcagtg acttgatata cttaatttgc
    17821 tcggcgacga tggatttcat cacctggcct accggggcgc ggtcgaccat gttgcgcagc
    17881 tctgcgctga tgttccggct gtgctgacgc cactgctttt cattctggcg tgcaatgtcg
    17941 gcggtaaagt tctcagcaac cttcgtcgcc cagggggtga tgatttcgct gtagcgctcc
    18001 agcgcatcca ttatttcggt aacgctatca tttgaaccat cgtagcgccc atttacgata
    18061 tccccgaccg cccgcgctat ctgccgtagg ctcgttcgat atcggatctc cgcctggcgg
    18121 ctctggcggt ttgtcgccaa gttcgccgat gcctggcggc gcttcgtctt cggcattctc
    18181 gatatcctcg tcggtaatgg atgccccgat gccggtgacg tcagagtttt cgcgcaggtc
    18241 ggtcatcgcc gccttacgcg tcatcaatcc gtcgcccagc gcggtactaa tcgcgttggt
    18301 ggtgtttacg gccaccgttg atcggtcaac gtctgacatt tgccatagcg ggttaaactc
    18361 aaacgtgaaa tcgtccggca gcggctttcc gagttccgag cggtgcataa tgtccagtat
    18421 ccggcgcatc ggcagccgta agcggcgctc ctgcaatgag ctcacccggt cgtaatagtt
    18481 ggcgaggtct gcatcaccag tagagaagcc tttcggggat tgaccgaaca accgcaccag
    18541 cgggatacca acggcaccgc taatctgctc agcaaactgc gaaagaatgt catccagacc
    18601 actaaagctg tactggtggg tttcgaactt atcccgcgag tccatgagcg tcataccttc
    18661 attgctctgg aactggcgga tcaggtcgat gttcttcagc aacgcctcga acgccgggcc
    18721 tccaagcgcg ataagctcgc gcaacttctc cacgctatag gtacgcagat gtgctttgta
    18781 gaccagctgc gccgcgccga cagtggcgct gtcgaacgca gtaagccgat cccagatacg
    18841 ctctacaacc gacattcccc attcgttttc ggtcatcttc tgctggaatg gcagcgtcac
    18901 cccgtcgaag cgaatcaggc ggctgtgatg gatgcgccag gccgggatgc ccgttgcagt
    18961 ggtcaccacg tcgtaaaact caggtttgcc gagatctggc cccatctctt taatgcggcg
    19021 ggtcaggacc gggttaatca tccagcggtc gagcgggaga atgcccttaa acttgccttc
    19081 tccaatggtt tcgagccgca gcggggtcat tggtgcctgc ccctcgatca tgatgaagcc
    19141 gaccgcgccg ccgtagaggc gagaccattt cagcacgtcg ttcagcgcat cccagatttg
    19201 caactcatcc agttgtgatt cgagaatgcc acgatctttt gcatcaattt ccgaagtgat
    19261 gcgaatgcct ttgcgggtca tatcatccgg gatagcatcg accgcttcgc cgataatcca
    19321 ggacgaacga taggaccatt ccaccagcat gcggttacga ctggtgaaat tagcccggta
    19381 ggtggatgct gagtgctggt taggtgtctg catccccacg cgggcaacaa agttctcata
    19441 gccatcagcg gtggcctgtg ccgttcgccg cgtggcttgt ttgtttcgtg ccatcaggcc
    19501 tgtctcccta gcagctccca gatgttcagg gctgaattca ttggcgcgta gctgatcatc
    19561 accgagtcgg caaggtttgg cgaccgggtt ccatcaggct gtttatcaat aacgattttt
    19621 cccacaccat taatggaata ggtcggctgc gaaagctcga tgatgagttt atctttgagt
    19681 gccatgctac tgctgattga gatgatttcg tccgggttgt aatccatacc ttcaaccacg
    19741 gcgcgccagg tattctgaaa aagtttacgt aaccgccacc agctctgggc tttggcgtta
    19801 gcgaagaagt ccttgttcag gcgggcggcc tgtccgttgt caccgcgcac cgcttcgtcg
    19861 tccggatcaa ataccgcgcc actacctcga aacggtgtgg cgagtattga cggtcggcgc
    19921 gcagcgttac gcagttcgtt gatagcgcgt gcatcgccgc gaacgccagc gcccaacccg
    19981 tcctcgtcaa agcgaaactc ttcgaggttg tcctgttcgc aaaagccgaa aaccttctcg
    20041 acggactgat aaatgtcgct gcccacaccg gaccattccc gcacattctc caggaggaag
    20101 ccatgacggg tggaaaagga atttttgtcc ctgccttcgt cggcgacgtc catcgcgccc
    20161 agtcgcttgc ctgttggctg gatgcccagc ttgatatgcg cgtcgacagc agcctgtacc
    20221 cattcggatg gaatcaggac gccttccgct gatgcactgt agttcaggtc aagttcctgt
    20281 gccaccacca ccggattgtc gattttctcg cattccctgc gataccactc ttcatccttg
    20341 cgaggatcat cccgccagtg gaatgtgaat accggtatct tcccgccatg acgcttctga
    20401 gcgaacgggt tcgccatgcc gttaactgaa ctcaggtcaa tacggcaacg cgtcgtttgt
    20461 gacaacgccg catcaatcag cagaggacgc tgaaggaatg cagactcatc aaccagataa
    20521 agcgtggtac ggtcaccacg gccaatatta tcgccagcct cacctttgat aacggcacca
    20581 gtttcaggaa actcaacgcg catgtatggt gcgtgcttct tttcgtccca cgaacctcga
    20641 aactctacag gtagtgtttc cacgaacttg cgcgccttcc agaacaatgc tttcgggtca
    20701 ccggtgctgt cgacgtattc ctctttacgg gagccgaaac cgataaccat ttctttgttg
    20761 aagagacaaa gcgagcatgc cagcccgatc gcggtccaac tgagccccat ttcacgggat
    20821 ttttcggtaa taccattctc ccgattaccc cagcgttcca taatccagtg gatccactcc
    20881 tcctgcttag ggaagagtaa aaacggaatg gtcaccggca ggccataatc aatattacgc
    20941 gggtccgttg tcatgcccca gtcgatgatg aactgagccg gattggttcg gtaaaactgt
    21001 tttagtgcag gcaatatttc aggattctgg cgaatgcgct gtaggcgttc catccgccat
    21061 tcaaaaacca tctggtaatc aggatgttta aaatcgaagg ggaatggtaa cggcatactt
    21121 agcccatcat ttttctatac gcctctgcag cctgctccgg cgttaagttg gtaatttctg
    21181 ttctgactgg tcctccgtca gcaccagtca cttcattttt gacattgtct ttaaacgcct
    21241 gaacagaaac atgacgcccg agcaactcaa ggtttttaac cttatcaggc catttgattt
    21301 tcttcaggag agcagcacta tctgctgatg ccatctcgac gacatccatt cctgatagcg
    21361 ttgtacgcca caccttaggc cagtctttaa tctgcttcag ttcgccattg gcaagcagaa
    21421 tgtcaagcac gtccatctga tcgatttcag tcagccgtcg caatacatag gcggcgtcta
    21481 tacctacctc ttcattgcgt tcagccttta gctcagcaat gcgatctgct atgtcaggtt
    21541 ttgacaggtt ctcgctacca gtggcgcgag cagttttctc gctgtaaccc gccctgatag
    21601 ctgcctgagt ggcgtttaaa tcttttaaat attccctgca gaacagttcc tgcttaggtg
    21661 tgagctgttt caaatcacac ctccgaaaca ttgctccaat ttc
//
LOCUS       DAEBXD010000063        21505 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEBXD010000063 DAEBXD010000000
VERSION     DAEBXD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.063"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KJL94_002563"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KJL94_002563"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBD4410868.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KJL94_002564"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KJL94_002564"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBD4410869.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVGF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="KJL94_002565"
     CDS             complement(3122..3421)
                     /locus_tag="KJL94_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBD4410870.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="KJL94_002566"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="KJL94_002566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBD4410871.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="KJL94_002567"
     CDS             complement(4633..5070)
                     /locus_tag="KJL94_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBD4410872.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="KJL94_002568"
     CDS             complement(5116..5457)
                     /locus_tag="KJL94_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4410873.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KJL94_002569"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KJL94_002569"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBD4410874.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KJL94_002570"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KJL94_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="KJL94_002571"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="KJL94_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4410875.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="KJL94_002572"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="KJL94_002572"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBD4410876.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="KJL94_002573"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="KJL94_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBD4410877.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13865
                     /gene="xylR"
                     /locus_tag="KJL94_002574"
                     /pseudo
     CDS             12688..13865
                     /gene="xylR"
                     /locus_tag="KJL94_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13973..14797)
                     /locus_tag="KJL94_002575"
     CDS             complement(13973..14797)
                     /locus_tag="KJL94_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBD4410878.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="KJL94_002576"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="KJL94_002576"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD4410879.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="KJL94_002577"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="KJL94_002577"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBD4410880.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="KJL94_002578"
     CDS             complement(18729..19202)
                     /locus_tag="KJL94_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBD4410881.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KJL94_002579"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KJL94_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBD4410882.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20353..21351
                     /gene="yiaK"
                     /locus_tag="KJL94_002580"
     CDS             20353..21351
                     /gene="yiaK"
                     /locus_tag="KJL94_002580"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBD4410883.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21363..>21505
                     /locus_tag="KJL94_002581"
     CDS             21363..>21505
                     /locus_tag="KJL94_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD4410884.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5399 a         5489 c         5283 g         5334 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagccc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgtcttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatat acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgttgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgctca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc aggcgttatg aggagataag
    21361 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    21421 aggcgctcga ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa
    21481 tcgacggcaa gaatatttat gcgca
//
LOCUS       DAEBXD010000064        21387 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEBXD010000064 DAEBXD010000000
VERSION     DAEBXD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.064"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..134)
                     /locus_tag="KJL94_002582"
     CDS             complement(<1..134)
                     /locus_tag="KJL94_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4410885.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="KJL94_002583"
     CDS             complement(236..1216)
                     /locus_tag="KJL94_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBD4410886.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="KJL94_002584"
     CDS             complement(1224..1934)
                     /locus_tag="KJL94_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBD4410887.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KJL94_002585"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KJL94_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBD4410888.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="KJL94_002586"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="KJL94_002586"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBD4410889.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="KJL94_002587"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="KJL94_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBD4410890.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="KJL94_002588"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="KJL94_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBD4410891.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="KJL94_002589"
     CDS             8511..8621
                     /locus_tag="KJL94_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBD4410892.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KJL94_002590"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KJL94_002590"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBD4410893.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KJL94_002591"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KJL94_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBD4410894.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="KJL94_002592"
     CDS             11503..12135
                     /locus_tag="KJL94_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBD4410895.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KJL94_002593"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KJL94_002593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBD4410896.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="KJL94_002594"
     CDS             15667..16599
                     /locus_tag="KJL94_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD4410897.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="KJL94_002595"
     CDS             complement(16640..18070)
                     /locus_tag="KJL94_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD4410898.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="KJL94_002596"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="KJL94_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBD4410899.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="KJL94_002597"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="KJL94_002597"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBD4410900.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="KJL94_002598"
     CDS             complement(19931..20200)
                     /locus_tag="KJL94_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBD4410901.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="KJL94_002599"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="KJL94_002599"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBD4410902.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="KJL94_002600"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="KJL94_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBD4410903.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DAEBXD010000065        21253 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEBXD010000065 DAEBXD010000000
VERSION     DAEBXD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.065"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..791
                     /locus_tag="KJL94_002601"
     CDS             <1..791
                     /locus_tag="KJL94_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBD4410904.1"
                     /translation="AAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQI
                     AVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEKV
                     TDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTDH
                     YYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFSF
                     FFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="KJL94_002602"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="KJL94_002602"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBD4410905.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="KJL94_002603"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="KJL94_002603"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBD4410906.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="KJL94_002604"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="KJL94_002604"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBD4410907.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="KJL94_002605"
     CDS             complement(4989..7223)
                     /locus_tag="KJL94_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBD4410908.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="KJL94_002606"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="KJL94_002606"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBD4410909.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="KJL94_002607"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="KJL94_002607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBD4410910.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="KJL94_002608"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="KJL94_002608"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD4410911.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="KJL94_002609"
     CDS             complement(12978..14318)
                     /locus_tag="KJL94_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD4410912.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="KJL94_002610"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="KJL94_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBD4410913.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="KJL94_002611"
     CDS             complement(16106..16555)
                     /locus_tag="KJL94_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBD4410914.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="KJL94_002612"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="KJL94_002612"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBD4410915.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="KJL94_002613"
     CDS             complement(17355..17684)
                     /locus_tag="KJL94_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBD4410916.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="KJL94_002614"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="KJL94_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBD4410917.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="KJL94_002615"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="KJL94_002615"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBD4410918.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="KJL94_002616"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="KJL94_002616"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBD4410919.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5670 c         5072 g         5285 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctggtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat gccgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatatcg
    16861 gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaatgc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccgc tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAEBXD010000066        20836 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEBXD010000066 DAEBXD010000000
VERSION     DAEBXD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.066"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KJL94_002617"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KJL94_002617"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBD4410920.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="KJL94_002618"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="KJL94_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBD4410921.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="KJL94_002619"
     CDS             complement(2649..3200)
                     /locus_tag="KJL94_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBD4410922.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="KJL94_002620"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="KJL94_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBD4410923.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="KJL94_002621"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="KJL94_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBD4410924.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="KJL94_002622"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="KJL94_002622"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBD4410925.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="KJL94_002623"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="KJL94_002623"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBD4410926.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="KJL94_002624"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="KJL94_002624"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBD4410927.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="KJL94_002625"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="KJL94_002625"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBD4410928.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="KJL94_002626"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="KJL94_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBD4410929.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="KJL94_002627"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="KJL94_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBD4410930.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="KJL94_002628"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="KJL94_002628"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBD4410931.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="KJL94_002629"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="KJL94_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBD4410932.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="KJL94_002630"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="KJL94_002630"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBD4410933.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="KJL94_002631"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="KJL94_002631"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBD4410934.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="KJL94_002632"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="KJL94_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBD4410935.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="KJL94_002633"
     CDS             complement(18174..18569)
                     /locus_tag="KJL94_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD4410936.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="KJL94_002634"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="KJL94_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4410937.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="KJL94_002635"
     CDS             19485..20078
                     /locus_tag="KJL94_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4410938.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="KJL94_002636"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="KJL94_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBD4410939.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4962 a         5226 c         5520 g         5128 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacatt
//
LOCUS       DAEBXD010000067        20748 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEBXD010000067 DAEBXD010000000
VERSION     DAEBXD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20748
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.067"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..554
                     /locus_tag="KJL94_002637"
     CDS             <1..554
                     /locus_tag="KJL94_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4410940.1"
                     /translation="EKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQRAKEAR
                     GGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLA
                     WDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYEL
                     YDKPEAVQEALAKVIPFFNTHLG"
     gene            572..1210
                     /locus_tag="KJL94_002638"
     CDS             572..1210
                     /locus_tag="KJL94_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4410941.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1501..2229
                     /gene="mpaA"
                     /locus_tag="KJL94_002639"
     CDS             1501..2229
                     /gene="mpaA"
                     /locus_tag="KJL94_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBD4410942.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2204..3163)
                     /gene="ycjG"
                     /locus_tag="KJL94_002640"
     CDS             complement(2204..3163)
                     /gene="ycjG"
                     /locus_tag="KJL94_002640"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBD4410943.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3282..3788
                     /gene="tpx"
                     /locus_tag="KJL94_002641"
     CDS             3282..3788
                     /gene="tpx"
                     /locus_tag="KJL94_002641"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBD4410944.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3832..5373)
                     /gene="tyrR"
                     /locus_tag="KJL94_002642"
     CDS             complement(3832..5373)
                     /gene="tyrR"
                     /locus_tag="KJL94_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBD4410945.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5521..6582)
                     /locus_tag="KJL94_002643"
     CDS             complement(5521..6582)
                     /locus_tag="KJL94_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBD4410946.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6579..7976)
                     /locus_tag="KJL94_002644"
     CDS             complement(6579..7976)
                     /locus_tag="KJL94_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBD4410947.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8132..9130
                     /locus_tag="KJL94_002645"
     CDS             8132..9130
                     /locus_tag="KJL94_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4410948.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9241..10113)
                     /gene="ompG"
                     /locus_tag="KJL94_002646"
                     /pseudo
     CDS             complement(9241..10113)
                     /gene="ompG"
                     /locus_tag="KJL94_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10186..11267)
                     /locus_tag="KJL94_002647"
                     /pseudo
     CDS             complement(10186..11267)
                     /locus_tag="KJL94_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11281..11940)
                     /gene="pgmB"
                     /locus_tag="KJL94_002648"
     CDS             complement(11281..11940)
                     /gene="pgmB"
                     /locus_tag="KJL94_002648"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBD4410949.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11937..14204)
                     /gene="ycjT"
                     /locus_tag="KJL94_002649"
     CDS             complement(11937..14204)
                     /gene="ycjT"
                     /locus_tag="KJL94_002649"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBD4410950.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14201..14824)
                     /locus_tag="KJL94_002650"
     CDS             complement(14201..14824)
                     /locus_tag="KJL94_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4410951.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14821..15243)
                     /locus_tag="KJL94_002651"
     CDS             complement(14821..15243)
                     /locus_tag="KJL94_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4410952.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15265..16040)
                     /locus_tag="KJL94_002652"
                     /pseudo
     CDS             complement(15265..16040)
                     /locus_tag="KJL94_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16058..17110)
                     /locus_tag="KJL94_002653"
     CDS             complement(16058..17110)
                     /locus_tag="KJL94_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBD4410953.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17141..17983)
                     /locus_tag="KJL94_002654"
     CDS             complement(17141..17983)
                     /locus_tag="KJL94_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBD4410954.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17970..18851)
                     /locus_tag="KJL94_002655"
     CDS             complement(17970..18851)
                     /locus_tag="KJL94_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBD4410955.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWITVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18872..20155)
                     /locus_tag="KJL94_002656"
                     /pseudo
     CDS             complement(18872..20155)
                     /locus_tag="KJL94_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20169..>20748)
                     /gene="ycjM"
                     /locus_tag="KJL94_002657"
     CDS             complement(20169..>20748)
                     /gene="ycjM"
                     /locus_tag="KJL94_002657"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBD4410956.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5063 a         5654 c         5047 g         4984 t
ORIGIN      
        1 cagaaaaacg aattaaagct gtagtgagta ttacgggtgt taatatcggg cgtctttttc
       61 gggaaggttt cagcaattat gatccgattg gcgtattgaa tgcaatggcg tcacaacgag
      121 caaaagaggc tcgtggtgga gaattacaga tcaacgaatt acttcccgcc agcctggatg
      181 cagcaaaagc ccatggactg accgaacgcg atgtttatga agcaactgac tactataaaa
      241 caccacgcgg gcaacaaccg ggaggtgcga caaaaatgtt gttctcacat gcacagaaaa
      301 cgctggcctg ggatgccttc gcatttactg aagtgttact gacccagccg gtgatggtag
      361 tagttggtga aaaagtcggt gccttcggtg cttatcgtga tggactggaa gtctatggcc
      421 gggcgatggt ttcgcaggat cgtcagttag tttcgttacc cgatttctca cattatgaac
      481 tatatgacaa accggaagca gttcaggagg cgctggcaaa agttattcct tttttcaaca
      541 cccatctggg ataagtttca tgagaaatat tatgaaaaat gtactgattc ttggtgctgg
      601 cggtcagata gcccgccatg tgattaatca actggcagat aaacaaacta ttaaacaaac
      661 tttatttgcc cgtcagccag caaaaataca caaaccctat ccaactaata gccagattat
      721 tatgggggat gtactgaatc atgcagcact gaagcaggcc atgcagggac aggatattgt
      781 gtatgccaac cttacggggg aagatttaga tattcaggca aattcagtga tcgctgcgat
      841 gaaagcctgt gatgttaaac gtctgatctt tgttctttcc ctcggtattt atgatgaagt
      901 tccaggaaaa tttgtcgaat ggaataatgc agttattggt gaacccctaa aaccattccg
      961 cagggctgcg gatgcaattg aggcttctgg actggaatat acaattcttc gccctgcctg
     1021 gctgactgat gaagatatta ttgattatga gcttacctct cgcaatgaac cattcaaagg
     1081 tacgatcgta tcacgtaaga gtgtcgcagc attaattaca gatatcatcg ataaacctga
     1141 gaaacacatt ggtgagaata taggcatcaa tcaacccggg actgatggcg ataagccatt
     1201 cttcatgtaa atcaattata tagataccat cgtactgtac tatgtgcagt acgatttatt
     1261 aaacttatta aggtgaactg atcaatcaga tgcgattaac atgtgtagaa ccagtaagaa
     1321 taaaatacgc catgtatata acattacgac aattctaata tctgcacatc aatgaaaatg
     1381 caaacaactt ccggcaaaaa agtgatacaa gatcgatgac atagcgttga agaaaaggat
     1441 ttgataatcc gcaaaatttg cacaacactg cctgtaatac ttcctgaacc agagacgatc
     1501 atgaccgtaa cccgctcacg cgccgaacgc ggcgcatttc cgcccggaac agaacattac
     1561 ggacgttcat tattgggcgc gccgttaatc tggtttcctg cccctgcagc cagccgcgaa
     1621 agtggtttga ttctggctgg cactcacggt gatgaaaact cttcggtcgt cacgctctcc
     1681 tgtgctctgc ggactttgac accttctttg cgccgccatc atgtggtgct gtgtgtgaat
     1741 cctgacggct gccagctggg gttacgggcc aatgctaatg gtgtggattt aaacagaaac
     1801 tttccggcgg cgaactggaa ggaaggtgaa acggtttatc gctggaacag cgccgctgaa
     1861 gaacgtgatg tcgtattact gacaggtgac aaacctggct ccgaacctga aacccaggcg
     1921 ctgtgccagc ttatacaccg cattcaacct gcctgggtgg tctcattcca cgatccactg
     1981 gcttgtattg aagatcccag acacagcgaa ttaggcgaat ggctggccca gtcgtttgaa
     2041 ttgcctctgg taaccagcgt cggttatgaa acgcctggct ctttcggcag ttggtgtgca
     2101 gatctcaact tgcattgcat caccgcagag tttccgccca tctcctccga cgaagccagc
     2161 gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc atcctaaaga tgcaattcgc
     2221 ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag ccaggtcggt ccgtcaagat
     2281 cggcgaaact gacctgcggc accagcggta aagcggcgct aatggcacga gaggtacaca
     2341 acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc ttcagtcgcc agcgcttccg
     2401 tcagaccacc ggttttatcg agcttaatgt taaccatctc atagcgccct ttcagcgcct
     2461 tcagattgct acgagtatga caactttcat cagcacaaat cggcaacgga tgaataaaat
     2521 tctccagcgc cgcatcgtcc tgcgcaggaa gcggttgttc aagcatcgca acgcctaaat
     2581 ccgccaatag ctggcaacgc gccgccaacc cttctgcacg ccaggattca tttgcatcaa
     2641 cgatcagcgt ggcatcgggc acagcggtgc gaattgccac catccgctca ctgataagat
     2701 ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt gatgcactat
     2761 tggccatctg atcaggcgta ccgatgacaa ccgtctgtgc agtgataact gtctcgggaa
     2821 gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgccgcg agatcccaca
     2881 atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc aactcctccc
     2941 gtgtcagccc tttctctaat tgcggcacga cgctcataat ttgcgccatt accgaggcat
     3001 cactttctcc ataacgcgga tacggcgtgc attcgccggt gcctttaata ccctcttctt
     3061 ccagttcaac caccaccacg cgcgcttcac tgcgacttcc ccgggcaatc acaaacgggg
     3121 tatgtaaggg ccaggcttcc tcaaatacct taacgattct catctttact ccttgttgtc
     3181 gcggtggtct gcggattaag cgcaaatagc gtttgctgtg ttattgacag ttagcataaa
     3241 ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa accgttcatt
     3301 tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc aaagcgcaga
     3361 cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt gcgggtaaac
     3421 gcaaagtgct gaacattttc ccgagtattg ataccggtgt ttgcgccgca tcagtacgta
     3481 agtttaacca actggcaact gagatcgaca acaccgttgt gctgtgtatc tctgccgatc
     3541 tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt atcaccctct
     3601 ccactttccg taacgctgaa tttctgcaag cctacggtgt ggcaattgct gatggcccac
     3661 tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga aaatgacaat gtgattttca
     3721 gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg gctgtactga
     3781 aagcataagt taatatgcct gatggtgttg caccatcagg catattcgcg cttactcttc
     3841 gttcttcttc tgactcagac catattcccg caacttattg gcaatcgcgg tatgtgaaac
     3901 gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct gggttaatac
     3961 cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat cttcgcccac
     4021 ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc tgtggacgca gctcataacc
     4081 gtccagttgt gtcagtgcgc gatagatagc gttctttaac tgccgcacat ttcccggcca
     4141 cgcataacgt gtaagtacag tattcaggtc agcggccagt ttcggacgcg gcacgccctg
     4201 ctcgtcggca aagcgggcga cgaacagctc agttaacggc ataatgtcct gcggacagtc
     4261 acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat cttcacggaa
     4321 cacgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca cccgcacatc
     4381 gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa gaaaacgcag
     4441 taatttcgcc tgcatccgtg gtgacatttc ccctatttca tccaacagca ccgaaccacc
     4501 gttcgcctgc tcaaagaacc ctttcttccc ttccggagca tgaccaaaca gttcactctc
     4561 gaccgcatct tctggtatag acgcgcaatt cagcgccagg taaggtttgc ccgctctggg
     4621 gcttgcctga tggcaggcgt aggcaaaaag atctttacct gtacctgtgt cacccgtaat
     4681 cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacgt gcttcatttt
     4741 cgggctgacg gcgacaattt ggctgaaggc gctgacgtcc tgggcggcga cattttgcaa
     4801 ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca ggacgtgttg
     4861 atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct gcccattaat
     4921 aacgacatgc tcgttatgcg aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa
     4981 gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt gcccaaaaag
     5041 ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag agagcacagg
     5101 ttcaggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca
     5161 cgggacagta cgcacatcgg taacacctgc aatacggcgt atttcggcca tcagactgct
     5221 gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg gatcaatctc
     5281 aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc gggtcagacc
     5341 gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa aaaagaacaa
     5401 ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac aatcattgac
     5461 acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc cgatattgca
     5521 ttatttttcg ctgggcgttt tgcctttttg cagagtttct ttcacctgac cgataagctg
     5581 acgacggaaa tccccaaggc gaggtttgtc atcgtcaatc cacggcagcg ggcggcaaag
     5641 ctccatagct ttaatcccga gtcgtaccgt cagaagtcca gcaccaattc cctgagctgc
     5701 gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca cttcgcgcac
     5761 cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca gacgcaaacg
     5821 gctgtaatac cccagttcaa tgccatacag cgtggcaatg cgattaatta aacgcagatt
     5881 gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa tcatcaacgt
     5941 tgattccgcc gccgaatggc tgatttcgcg ccgcgcctgg gcatctaaaa ctggctggac
     6001 caaatgcgca tacaggctga ccacttcacg atcgttctgc gtttcatgga ttgaggcata
     6061 ccagcgttgc agcgccggat gcgactgatc aatacccgcc tgctgcgcca gtttttcgca
     6121 aaatgcgcga cctttgcccg tgccgtggct atgcaataaa tcacgcgctt cgtcgcgttc
     6181 atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag aacctacgcc
     6241 agcgccgata atcaatgccc cagcggcaca tccacccagc gctacccagt cctgggtttg
     6301 ccaggcattc attgtccact gtataccctg cccgacaacg cttgcgccaa acagagccag
     6361 cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg cgtccattac
     6421 cgcttcgact tgcccctctt cttcctgcgc ttcgtcgagc gtggccgggg caaaattttg
     6481 cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt ggattctgtt cgacctccag
     6541 cggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt atctcctatt
     6601 aaaaattcca gcgcggcatc aagacgaata tgcggcagcg gtttgtcgac atccatcacc
     6661 tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc agaacgcctg accgggcaaa
     6721 cgcgcaggaa cttcaccggg gtaaacggtg agaggcgcac catcgctgag ccggttaccg
     6781 cgcaatgccg gaattttctc gccgttgacg tcaataatgc cgctggtggt cgcctgcacc
     6841 gaggccagcc ccaggcaatc catactaatc ctttcaaacg ccgcattttg ccaggcatcc
     6901 tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct gatcgaccgt tacatggtcc
     6961 gctttcatcg cggcaaacag cagtttgtcg ataaccggcg agaataagcg gcgaaacagc
     7021 gtgcgctgcc cgtagtggaa actttgcatc agctgcgtca gtgccagacg catatcatta
     7081 aatgcctgtg gcccactgtt gagaggttgc aggcaatcca ccagcacaat ctggcggtca
     7141 aagcgcagaa aatgattctt atagaacccc ttcaccactt tctcgcagta ataattaaac
     7201 cgctcgcgca gcattccggc attggtgtgc ttatcggcct gcgccagttt ggactcgtcc
     7261 caggcatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc catatctccc
     7321 ggcaagacaa agcgcccagg ctgaataaag tgcagcccct gctgtttaca gtggtggaga
     7381 taatcggtcc acacggcggc aatgtctgcc agccggtttt cgtcggcagg cgctagcggg
     7441 tcaagtcctt cgcacatcat tcgccatttc gccgaccatt cgccgcgctg accattgagt
     7501 aagcccgtca tctggcgcga ccagcttaag taatcctgcg ccagcatcgg caggtcgagc
     7561 aaccattcgc cgggataatc cacaatttcc agatacagcg tggaggtatc cttaaagtgg
     7621 cgcagcagcg aatcgttcga tttaaaacgt aacgccaggc gaatttcact gacgccgcgc
     7681 gtaggcgtcg gccaggcggg aggatcacca tataactgcg ccagtccttc atcataggtg
     7741 aagcgcggaa tgccaaagtc acgctgggga atgcgtttta cgcccagcaa gcgctcttca
     7801 cgcaccgcgc ttaacagcgg caaacgtgct ccggcatgaa tattgagcaa ctgattaacc
     7861 atcgcggtga taaacgctgt tttgccgctg cggctaagtc cggttacagc gaggcgcaga
     7921 tgtctgtcga caccccgatt caccagcgca ttgagttcat ttttaagtcg cttcattgct
     7981 gtcctgtgct gctctgaaga ccagaaaaaa aatcattcgt aaggatatga atgtgggggc
     8041 caggccattg attatcaact gcccgtgttg tcatcgacgg gcgaactcca taagatgtgc
     8101 agcattcacg ttcggacgga gttcctgacc tatgtcccct actatttatg atattgccag
     8161 ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg aataagcaaa ctaatatctc
     8221 cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa ttacagtatc agccaaacaa
     8281 gctggcccgc gcgctgacct cttcgggttt tgatgccatt atggtgattt ctacccgttc
     8341 gaccaaaact acggcgggta atccgttttt ctctgaagtt ttgcatgcca tcaccgccaa
     8401 agcggaagaa gaaggtttcg acgtgatatt acaaacgtcg cacaatccgg cagaagatct
     8461 gcaaaaatgc gaaagcaaaa ttaaacagaa aatgattaaa ggcattatta tgctcagttc
     8521 gccagcggat aagtcatttt ttgcccaact cgataaatac gatattccgg tggtggtaat
     8581 tggcaaagtt gaaggtcaat atgcccatgt ttattctgtc gataccgata attttggtga
     8641 cagcattgcg ttgaccgatg cattaattga aagtgggcat caaaatattg cctgcctgca
     8701 tgctccgctt gatgtccatg tttcggttga tcgcgttaat ggctataagc aaagtctgtc
     8761 cgcgcataat attgcagtgc gtgataaatg gattgttgac ggcggttata cccatgaaac
     8821 agcactgcaa gcagcacggc aattattaag tcagtcaccg ttgcctgagg cagtgtttgc
     8881 cactgacagc ctgaaattaa tgagcattta tcgtgcggcg gcagagaaaa atattgctat
     8941 tccgcagcag ttagcggtgg tgggttatag taatgaaacg ctgtcgttta ttttaacgcc
     9001 tgcaccaggc ggcatcgatg ttccgacgca ggagttaggt caacaaagct gcgagttatt
     9061 attccgctta atttcgggaa aaccgtcacc acagaatatt accgttgcca cgcatatgac
     9121 gctgaaataa ataccagcta ccttgcctga atgccggatg cgatgctggc gcatcttatc
     9181 cggcctacat ttatagatcg gataaagtac tcgcgccgca tccggcaatt tagcccatta
     9241 tcagaacgag taatttacgc cgacacctgc ataatggaat ttatcgctgt cgccttcgtc
     9301 gtgatcctgc cactcaaacg cgtattccag cgaaacggaa agtccgttct ggaaatcata
     9361 accgtaaaat aaacccacac ggttaaaatc atggccttca cgttcaatat catcctgcca
     9421 gtcccagtta ctctagcgat ccagcccaat gcgcgtatac ggcgtcaccg agtggttgcc
     9481 gagcgtcagc ggcaaatagg cgcgaatttc ttgtgtggaa aactcaccgt tattgcggct
     9541 gtcgtccata ttgaagccgc gctcgagata atagttcact cgcaaagcaa ccgtttcgtt
     9601 gaaggtatat tgcagacctg tttccgtttc gacacgggta tcagcgtaac cggtcgtgtt
     9661 caaatcgttg gcaaatttat acatcgacaa ccaaccgttg aaacgtaaat catcagtcag
     9721 tttcacatcc cagtctggcg cgattttcca gcgctgcata ttcgccgtgt ctttgcccgg
     9781 ttcatcaacg tagtgataac cataattacg gaaaccgccg gtcaggccga aactgaaatc
     9841 atcgctttcg aggaactgat aatgcacctc cagctccggg cgatcaaacc tcgttccacg
     9901 tttacccgcg ctataatcta ccggcccttc ctgataatag gccagagcaa ttctccacgg
     9961 cccgttggcg gcattaaaat agactgaagg ctccgccagc ccatccatat cttcgccata
    10021 accctcgacg ttttctattt cgtacatcgc accgatatta aagtgccagt cgctcttttc
    10081 ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt gcggtacagg gtaataactt
    10141 tttttgttcc ttgtattatc ctgggggaat gccttccccc tgtatttagc gaattgctat
    10201 ttccgtttct gcatcaaaga aatgacattt cgtcatatca aaatgaatag tgatattttc
    10261 tcctgcatga taatcattta acgcaccagc acggaccact aactcgtgcc cccaaccgtg
    10321 gtgtagagca taaattccgc accggttaat tctgccacgc taattttggc ggaaatgtta
    10381 ttttcctctt gcgcgtccgg atgaatatct tccgggcgta ttcccatcac gatgggctta
    10441 tgcaaacttt cctgtgtttt cagaaccgct aatttctctt cgagaatggt taatttaagc
    10501 gtttccgtaa cgaatttatc gccatcgatc gtgccgcgaa taaaattcat cgctggtgat
    10561 ccaataaatc cggcaacaaa catattcgcg ggttggttat aaacggtttt cggcgcacct
    10621 acttgctgaa caatcccgtc tttcataatc acaatccgcg ccgccatggt catcgcttcg
    10681 gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt tctgatgcag cttgctgatc
    10741 tctgcacgca tttgtacgcg cagcttggca tcaaggttag agagcggttc atccattaaa
    10801 aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca cacgctggcg ttgcccgccg
    10861 gaaagcgcgc ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg cgccgcccag
    10921 ttcacccgct catcaatcac ctctttggcg attttttgca tcttcaggcc aaacgccatg
    10981 ttgtcgtaaa ccgtcatatg cggatacaac gcgtagttct ggaacaccat cgcgatattg
    11041 cgtgctttgg ctggaacgtc attcattcgt ttgccgtcga tcaacagatc gccgccgctg
    11101 atctcctcaa gcccggcaat catgcgcagg gtggtcgatt taccgcagcc cgacgggccg
    11161 acaaatacga tgaactcttt atcggcaatt tccaggttga agtccttcac cacatgcacc
    11221 tggttatcgt agattttttg aatatgttgt aacgaaagct gagccatgtt gattcctttg
    11281 ctatacgttt tgccagaagg ccgataaccg cgaccaggtc agggagtccg ttgagggcaa
    11341 cagtaattgc gccccggtta agcccgcacc gatccccacc gagcgcatac cgctggcgtt
    11401 aatggcgtca atgcccgcct gcgcatcttc aatgccgata catgcctgcg gcggcacgcc
    11461 cagcccggca caggcagcga gaaagatttc cggatccggt ttcgagtttt taagttggga
    11521 agcatccgcg cagaaggtga aaaactcgcg cagctccagc gccgctaaaa tcgtcggcgc
    11581 attcagggag acggaagcca gccctaccgg aatctgctgt gcacggagct ctgccagcaa
    11641 attgcgaata ccgggcagca cagcattaac cgtcagctcg cgcagtgagt ggacgtaaag
    11701 cagatttttg cgatacgcca gttgcgcccg ctcctgcggg ttaaagtcgc cctctttgcc
    11761 cccgtgttgc agaatgcgcc gcagagactc atcgcggctg atccctttta gagattcgtt
    11821 aaactgcgca tcaatactga tgccaatttc agcggcaatc tgctgccacg cctggaaatg
    11881 cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa attacccctt gcagtttcat
    11941 tcatcctcct gatgcgtggt agcggtccca ttgaagggca aaataaaatc acccaaacag
    12001 aaaacagatt cttcagccac ggatataagc tgaccgttca aacgcagtga aacgggcgca
    12061 gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct gtaattcgca gccctgccag
    12121 aacagaggga aagacaactg ttgccactgc tcaagtaacg ccggatttag atgcaattca
    12181 ccgtcacgca cgctcacccc ggcaaaaccc tgaatcgccc ccagccagat agcgccagtt
    12241 gcggcagcat ggataccatc atcacaacta tgcggatcag caccaagatc gatttcagtc
    12301 ccctcgcgcc agaactgata actttgggtc agcaggccac agcgtgcagc aacaatgccg
    12361 tggattgctt tacttaatga cgagtcgtga atagtgcgcg gttcataaaa ttgcagattg
    12421 gcaagacacg atgccgatga gaactgctct ggcagcatgt aattgagcat caccacatca
    12481 gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg aataatccag cagaatggtt
    12541 tgcttccccg ccgccgcttt gtatttcgcc agattaatcg ccggcttagt cataaacgaa
    12601 tcatcctgcg gcaaaacacc gtcgggctga atttctggca gccgcagttc tttaaggaac
    12661 atttcggcac gatgaataaa cgcgtcgtca ctacagccaa actggcgggc aatactcagc
    12721 gcctgctgga cgttgtaata caccatatag ctggtgaagg cgttattatt gacatgttcg
    12781 gtatattcgt ctggcccaat aacatcatga atttccagac ggtcgttaac cctcaccgcg
    12841 cggctaatcc agaactttgc agtttccaga agtagcgcca tgccttcatg cgcaatgaaa
    12901 ctttcatccc ccgtggtctg ccagtattga ataaccgccc aggcgatatc ggccaccaga
    12961 tgatgttccg cctgcgccga ggccactttt tgccgcagcc cggtgcgaat gttaatggcg
    13021 gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa tagcgcgccc
    13081 tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca agttgtgcca gcgataacgc
    13141 agtaaacttc gggcaaccgt cggatcgcta aacagatgga acggcagcaa aaatacttct
    13201 gtatcccaga aaacgtggcc tttgtagcct tcgccggtta agccttttgc cgcaatactg
    13261 ctgcgctcgc cgtgagccgg cgtcatgatg cgcagatgat aaagcgcata atctaacgct
    13321 tgctgatcgt gcgcatcgcc gccatttacc gtgatacgac gtttctgcca ccattgacgc
    13381 cagttttctg ttgatgctgc aagatgttgg tcgtaactct gttgcgcgca catttcaagc
    13441 tggcgaagcg acacgctgcc ccactcgtct aaagcagctt gcctgtcatc ccgccagtcg
    13501 atccacacca gtttttgcaa cgtcactgtc tcgcctgcat gaagctgcgt actggtatgt
    13561 tgcagtaaac ggcgctcttt ggcggtatag cattgctgca catcaccgct cacctggcag
    13621 caacaactga tggccacatc actgcgccca tcctgggtgg tgtagatccc ctgcatcaaa
    13681 tgctgaccaa acacccgcac ctgggtttcg tcgagatgct ggcgaccgtg gttggtttgc
    13741 gtggcgtcaa tgcctgttga aatcagcact gacgcgtcgg cgtccagtgg cataatagtg
    13801 atttccagcg caatgagcgg cagttggtct gccgaaacaa agcgacggct ggcgatggtg
    13861 taacctgcgc cgttgctggt acgccagaca acgctacggc gtaattctcc actggcaaag
    13921 tcaagttcac gctgccaggc ttcgcgggat aacgagaaaa cctcaccatt tatggcaatc
    13981 tccatcccca agatatcagg cagattcacc agttcgttga tttcaccttt tcccgcccga
    14041 tgatacagcc ccgccagata catccctcgc gtctggcgcg tgtaatcttc ttcatggctg
    14101 gcgcgaagcc caagatagcc gttcccctgc gccatcagcg atgcatactt gttcagggta
    14161 tgctggctga aatggggttc tgataacgtt actggcctgg tcataattcc acacgcgtcc
    14221 ctgtttctgc ggattgatat aacgccgcca ccagttgctg gatgatgtac ccctgctcgg
    14281 cgtcggctat catcacgggc ttgccctgta cgtgattgat aaaggcttcc atgctgcgca
    14341 aatggcggtt gtcgtctgcc atttcccgtt gcatcagcgt cattaattca ccgttgttat
    14401 cggtgtagat atgtgctgga aacagcgtcg caccggcttt atcaccacaa aagctgacgt
    14461 tcataatcaa ctgttcgcgg atgttgagtg caaatgacgt ttccagccac agaatgccgc
    14521 cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac gctgtaagtt gccggatccc
    14581 actcgccaaa ttgaccacag ctcttttgcg tgccgatctt ttgaaaacta tgcgcattca
    14641 cgcttttcac cgccggaaag cccagcacat acatcgcagc atccagcata tgaatgccga
    14701 tgtcgatcag caggccacca ccctgcagtt ctttattggt aaagacaccc cagccgggaa
    14761 cgccgcagcg acgcagggcg cgggcggtgg taacgtaaat ctctcccaaa acgccgttgg
    14821 tcacctgttc acgcagctgt tgcgtatcga gcgcaaacga tggtgaaagt cgtaggccag
    14881 caccttgccc agtttgcgcg cggtatcgca catttcccac gcctgttctg tcgtcatggc
    14941 gggcggtttt tcgcacatca catggcagcc tgcttccagt gccatcaggg tatgttcgta
    15001 atgaaaacgg ttaggtgagc cgacgctaac cacatcaggt ttcaccgcca gcagcatggc
    15061 ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct gcgcctggga
    15121 aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt agtacgaagc
    15181 atgaacctta tccgccacct ggcccgcgcc tattatcgcg acccgcagcg gagagcttgt
    15241 cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg aatcacggta
    15301 cgcctgggca ggatcttctg cccggatacg cccttcatac accacataac cctgatagtt
    15361 atcggcgcgc agctgttcaa acagcgcgtg gaaatcgagg gtgccactgc ccggctggta
    15421 acgatggtta tcggcaatat gcacatgacc gagcaagtca cggttgtcgt gcagcgcctg
    15481 cgccaggtta tcttcttcga tgttcatgtg atagaaatcg ccgataatct gcacatgttt
    15541 aagattgttt tcgacgatgt aacgacgagc atcggcgagg gtgttgatca tatgatcctg
    15601 atagcggttt aacggttcga gatacaccac ggttccggta cgcgcggcga cctgttccag
    15661 tacgcgcagg gaatcactca ccagtttgcg gtcgccgtcc aggctacgcg gcgaggtcat
    15721 cggcggtaag cggaaggtaa acatgcccca cgctgccgga aaccccccac ttctgccagc
    15781 gcctcgagaa tgcgttcgat ctgctttaag ccattaagac gacgctcttc gataaagtca
    15841 ccaatccagc cgtcatagcc gccgcaggcg gtggtcactg gtaaaccggt ttctttgatc
    15901 gccgctttga cttcttcgat gttgttgacc agcagtttgc cgtcaatctc aaaaccatcg
    15961 aagcccatct ctttgatata acgaaatctc tcgcggatat tttccggaaa gaatgcctga
    16021 ttctgtgtgc cgattttcat tttttcattc ctttgaatta aaacgtaacg cccattttga
    16081 tgctctgttc cggatgctga tcgacatact gcatgtagct ttccgggctg ttggcaaagg
    16141 tcactaccgg gtcgattaaa tcttcgcaat tgagataacc gttcatcagc agttcccagc
    16201 aggtttcttc aatacgctta cggctccagc gcggataatc cgggttcggt tcgctgcacg
    16261 cgcgggagaa aacaattttg gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt
    16321 cagcaaatgg tttggcaaac gcgacatagg agatggtgcc gccgtaggcc aggccgcgca
    16381 gcgccgattg cagcgcgtcg gcgtaaccgc tggtttcgat aatcacatca gccccctgct
    16441 tgccggtcag cgttttgatc tctttgccta cgtcagtgcc aatggggtta aggcagaaat
    16501 cagcaccgtg gcgacgggca atatcacagc gatgggcgat aggatcgacg ccaattacca
    16561 ccgatgcgcc agcgcgtttc gccagttgga tggcaatttg accgatcgcg ccaagcccta
    16621 ccaccacgac aaaatcccct acgcgtacgt tggcatcgcg cacgccgctc atggcaaact
    16681 gcgccgggtc gtaacagacg gcgtttttcc aggagctgcc ttgcggcatt ttgcgcagct
    16741 tgtagttatt cactgcgtta atgatgaccg tctcggagag cgggccgtag ccgcatacgc
    16801 tgtcgcccac cgcgtagtct gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca
    16861 tgttgccaag ctggaatttg ccaaactcaa tgccgcgcgg cgcatctgcc ggacgcgggg
    16921 tgaacatctg ccactcgccg ttaaagtctt catcgataaa cgggctggcg gcgcggaagt
    16981 cgaccacttc cgttccgtgt ttcggtgcgc cgaaacgcac gcggatcttc acttcattgg
    17041 ctaaaatcgc ccgatcttca tactcaacca gcgcagcaac acgcggtgct gtggctacta
    17101 actttttcat gtaaaactcc ttgtaaagaa actggccgct ttagcccttc acgccaccgg
    17161 cggtcaaacc actcttaata aaacgttccg aaagcgcata cataatcacc accggcaatg
    17221 cggtcaccag cgaggccgcc atcatccgtc cccagatgta atcaggcgta ctgaacagcg
    17281 cgttcaggcc cactggtaag gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca
    17341 gatagtcgtt ccacgccacc ataaagcagt agacgaacac cgatatcagc ccggacatcg
    17401 ccaggggcac ggtgatacgg aagatgattt gcagacggtt gaggccatcc atcatcgccg
    17461 cttcttcgat ctcatcaggg atggtgtcga agtaactttt cagcataaac acggcggtgg
    17521 gcagggtttg cgtcaccatg gtaatgatca gcgccatctc ggtgtcgtaa ataccaagcg
    17581 cggtgatgat tttgaacaga ggcaccacca gcaaaatgcc agagaacata tacaccgtgt
    17641 aaaagctggc gttgatggtc atccggcctt taaagcgcag gcgggaaagc gcgtaagctc
    17701 cgagaatgcc gagaaatacc gccaccacgg aggaaaccac cgacaccacc atgctgttac
    17761 ggaagtagtc gacaaacggg aaaatcatcg ggttaaaaat gtcgacgtaa tgctccagcg
    17821 tccactgttg cggcagcagc gtaggatgca gcgatatcgc ctctttcgcg cttttgaacg
    17881 aggtcatcag catcacaaaa aacggaaaca gcgtgatgat gagaaacagc gccagcccgc
    17941 agtaaaaacc gatacgactg agcgtgcgtt tatttgttgc cattgaggtt cacccgtttc
    18001 ctggtcagca gaatgacagc gaaaataatg atgaagagca ccaccgagat cgccgccgct
    18061 ttccctaagt cattaaaggc aaaggcggtt ttgtagagat agacaccaag aatatcgacc
    18121 ttggttgtca gcagataaac atcagcgaac atgtagaaca tccagatggt gcgcagggtc
    18181 accaccgtcg ccaggaccgg cataattgcg ggcagcgtga cgatgcgaaa ccgctgccag
    18241 gcattagcgc catccatctc tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc
    18301 gccagaaacg agataaaggc atacgggaag tagcgccaga tggcgaacag caccaccagc
    18361 acaaagctac tgcccggatt atcaaaccac agcggtgcct gctcatagag atgcagaaga
    18421 tcgacgccga ggtagttcac aatgccgtag ccgttgttga acatgtattt ccaggcaaac
    18481 accagcgaaa tggacggcgt tacgtaggag aggatcacca gcgaacgcgc ggttttgcgc
    18541 aggcggaact cgcggttgaa aaacatcgcc acggccagcc cgagcacggt gctgcccgcc
    18601 accaccagcg ccgtatacca gaccgtaatc cacagcgagt gccagaagcc gggatcggag
    18661 agaatgcgca catagttact caccccaaca aacgttgact cgatgttggg attgagcggc
    18721 agacgtaaaa aactgatttc gatattcgac accatcggcc acgccaccag accgcccagc
    18781 agtaataagc tgggcgcgag aagcagcagc gcaaacggca tatcggaacg acctgaaaaa
    18841 agcctgttca ttgcggattc ctgtcctggg tttagtgctg ttcgatcagt tcatccagct
    18901 ttttctggct cgcttgcagc gtgccaggga gatcggcttt acccacggtg acgttatgca
    18961 ccatggagct caccacacca gaacccgtca catcgcccat gcgggtaaag tttttatccc
    19021 ctactgcgcc aaagacctga atatttggca gttcaccgat tagctgattc ggtagttcac
    19081 ccagcgcctt aataacgtcg ttgtctttcc aggtggcggt agtgacaacc gctttattca
    19141 ccggcagcgc agcacctggc gacatcatca cccagtcggc aatgttgtcc gcctgctcca
    19201 taaaggtgac aaatttttct gctgcttcgg tcttttgtcc ggcggtaatg gtcagcgagg
    19261 tcaacatgcc gtagaccgca gagtttttct cggttggcac cacgaaaccg acgtttttcg
    19321 ggtcgccttc tttaatcaca gccggaagga tataggtgga gtaaatcgcc atcggcgcgg
    19381 tgccgttcat aaaggcgtct ttcacttcca tgatgtcgtt agaacccggc atggtgttgg
    19441 cagcaaggtc gcggtaatag gtcagcgcct gcatcatctc tggtgtatca agggtgattt
    19501 tgccttcggc gttaaagacg ttggcctagt tggataacgc aaactgggag aaggattgtt
    19561 ccgtcagcac gctttctgcc gtaggcagcg caataccgta ttttttattc gccgggtcat
    19621 tcagtttctg tgcaacgtcc agcagctgtt gccagttttt cggctcctcc agccccgctt
    19681 ttgccagcac atctttgcga taccagatgt cgccaatcca ggcgctgaca ggaacaccgg
    19741 tccatgcgct accatcttcg gtacgcacaa tacgcagtac gccatcgtaa aacgcgcctt
    19801 caccaacgtt gctgatgacc gtggcaaccg ctttgcgatc gataagctgc tctttatcca
    19861 taactttggc gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa
    19921 gggtgatgac tttggtgtta tgagcgtctt cttccacggg cacttgttta acggtgatac
    19981 ctgggttttc tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct
    20041 ccaccgaaga atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg
    20101 aaatcagggc gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat
    20161 gttccttttt atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat
    20221 atatcaactt cactaatcag atcccgacca tgcagatcag taatatttac atgctgaata
    20281 tttttactga cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata
    20341 cgcattacgg atgaattaac cgtatcgatg gtaaaattat tatccggatg aaactcgtta
    20401 tggctgcgac gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta
    20461 gcttcatcgt tcagttctcg tgttatctct tcactgtgat attttttacg gttaatcgca
    20521 cggttatatc cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga
    20581 atatatatcg cgggtacacc ggggaaactt aacaaaatcg catgggcaag gataaacctg
    20641 gtgcaacgtt cttcatcgct actctcgcgg cggcttaacg cattcatata ggtgacattc
    20701 atctcatagg gactgcgcgt gccgtcggga ttatttttcc agtttact
//
LOCUS       DAEBXD010000068        20713 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEBXD010000068 DAEBXD010000000
VERSION     DAEBXD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.068"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="KJL94_002658"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="KJL94_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBD4410957.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="KJL94_002659"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="KJL94_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBD4410958.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="KJL94_002660"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="KJL94_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBD4410959.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="KJL94_002661"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="KJL94_002661"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBD4410960.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="KJL94_002662"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="KJL94_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBD4410961.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYEALGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="KJL94_002663"
     CDS             complement(8217..9110)
                     /locus_tag="KJL94_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBD4410962.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="KJL94_002664"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="KJL94_002664"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBD4410963.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAEH
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="KJL94_002665"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="KJL94_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBD4410964.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="KJL94_002666"
     CDS             complement(10241..11473)
                     /locus_tag="KJL94_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBD4410965.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="KJL94_002667"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="KJL94_002667"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBD4410966.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="KJL94_002668"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="KJL94_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBD4410967.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="KJL94_002669"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="KJL94_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="KJL94_002670"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="KJL94_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBD4410968.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="KJL94_002671"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="KJL94_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBD4410969.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="KJL94_002672"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="KJL94_002672"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBD4410970.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="KJL94_002673"
     CDS             complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="KJL94_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBD4410971.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4982 a         5724 c         5033 g         4974 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa gcgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgt gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac aacgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttctag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 agcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttagccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAEBXD010000069        20703 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEBXD010000069 DAEBXD010000000
VERSION     DAEBXD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20703
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.069"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..129)
                     /locus_tag="KJL94_002674"
     CDS             complement(<1..129)
                     /locus_tag="KJL94_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD4410972.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNT"
     gene            complement(307..1956)
                     /gene="actP"
                     /locus_tag="KJL94_002675"
     CDS             complement(307..1956)
                     /gene="actP"
                     /locus_tag="KJL94_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBD4410973.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1953..2267)
                     /locus_tag="KJL94_002676"
     CDS             complement(1953..2267)
                     /locus_tag="KJL94_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBD4410974.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2467..4425)
                     /gene="acs"
                     /locus_tag="KJL94_002677"
     CDS             complement(2467..4425)
                     /gene="acs"
                     /locus_tag="KJL94_002677"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBD4410975.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4818..6254
                     /gene="nrfA"
                     /locus_tag="KJL94_002678"
     CDS             4818..6254
                     /gene="nrfA"
                     /locus_tag="KJL94_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBD4410976.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6299..6865
                     /gene="nrfB"
                     /locus_tag="KJL94_002679"
     CDS             6299..6865
                     /gene="nrfB"
                     /locus_tag="KJL94_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBD4410977.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6862..7533
                     /gene="nrfC"
                     /locus_tag="KJL94_002680"
     CDS             6862..7533
                     /gene="nrfC"
                     /locus_tag="KJL94_002680"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBD4410978.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7530..8486
                     /gene="nrfD"
                     /locus_tag="KJL94_002681"
     CDS             7530..8486
                     /gene="nrfD"
                     /locus_tag="KJL94_002681"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBD4410979.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8506..10224
                     /locus_tag="KJL94_002682"
     CDS             8506..10224
                     /locus_tag="KJL94_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBD4410980.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10217..10600
                     /gene="nrfF"
                     /locus_tag="KJL94_002683"
     CDS             10217..10600
                     /gene="nrfF"
                     /locus_tag="KJL94_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBD4410981.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10597..11193
                     /gene="nrfG"
                     /locus_tag="KJL94_002684"
     CDS             10597..11193
                     /gene="nrfG"
                     /locus_tag="KJL94_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBD4410982.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11535..12848
                     /gene="gltP"
                     /locus_tag="KJL94_002685"
     CDS             11535..12848
                     /gene="gltP"
                     /locus_tag="KJL94_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBD4410983.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12926..13615)
                     /locus_tag="KJL94_002686"
     CDS             complement(12926..13615)
                     /locus_tag="KJL94_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD4410984.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13709..15856)
                     /gene="fdhF"
                     /locus_tag="KJL94_002687"
                     /pseudo
     CDS             complement(13709..15856)
                     /gene="fdhF"
                     /locus_tag="KJL94_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(16054..17520)
                     /gene="mdtP"
                     /locus_tag="KJL94_002688"
     CDS             complement(16054..17520)
                     /gene="mdtP"
                     /locus_tag="KJL94_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBD4410985.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17517..19565)
                     /gene="mdtO"
                     /locus_tag="KJL94_002689"
     CDS             complement(17517..19565)
                     /gene="mdtO"
                     /locus_tag="KJL94_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBD4410986.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            complement(19565..20596)
                     /gene="mdtN"
                     /locus_tag="KJL94_002690"
     CDS             complement(19565..20596)
                     /gene="mdtN"
                     /locus_tag="KJL94_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBD4410987.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
BASE COUNT         4644 a         5456 c         5667 g         4936 t
ORIGIN      
        1 cgtgttttta tgatgtttgc tgtaattatc tgattttgtt ttctttgtat gtgatgaact
       61 tgtgatagag tggttatcgt taatctggca aagagggaaa aacatattat ttaaaccatt
      121 atagcgcata aaatatcctt ataattaaca agaaagaaaa ggcatattct ctgcattatc
      181 attttctacg actgtcaaaa atcgctcatt ttttaatgag tttatttgtt taatattatg
      241 ggaaaaggtg atgcatgttg gagaggaagc gtattccccg gtcaggcgac cggggaaggg
      301 gagagattaa tgtgcgcggc cttgctcaac gccaaagccg gtctgggagc ggataaactg
      361 cgcgcggaac agttcacgct cgcgcgcccc ttccgctgag ttatcggttg ccgagaagaa
      421 ccagatgccg aggaatgcca cgctgataga gaacagcgcc gggtattcat acgggaagat
      481 ggctttttcg tgaccgagga tctgtaccca aatagtcggg ccgaggatca tcagcaccac
      541 tgcggtaatc agccccagcc agccgcccag catcgcgcca cgggtagtca gtttcgacca
      601 gtacatggaa agcagaatga tcgggaagtt acagctcgcc gcgatggcaa acgcaaggcc
      661 taccataaag gcgatgttct gattctcaaa cagcacgccg aggataatcg caatcacgcc
      721 gaggatcagt acggtgattt ttgatacccg cagctcttca cgttcggtcg cgcctttttt
      781 gaagacgtta gcgtacaagt catgcgaaac cgccgatgcg cccgccagtg tcagatccgc
      841 aaccaccgcg aggatagtgg cgaaagcaac cgctgaaata aaaccgagga ataggttgcc
      901 gcccactgca ttcgccaggt gaacggccgc catgttgtta ccaccaatca gatggcccgc
      961 cgcgtcttta tattccggat tcgcaccaac aagcatgatc gcgccgaagc cgataataaa
     1021 ggtcagaata tagaagtagc ccataaatcc ggtggcgtag aacacgctct tacgtgcttc
     1081 gcgggcatcg ctgactgtaa agaagcgcat cagaatgtgc ggcaagcccg ccgtaccaaa
     1141 catcagtccc agccccagag agagcgcgga gatcggatct ttcaccagtc cgcccggttt
     1201 catgatgtcg acacctttcg ggtgtaccgc catcgcttcg ctgaacagat tgttgaagct
     1261 aaagccgacg tgtttcatca ccataaaggc cataaagctg gcaccgaaca gcaacagcac
     1321 agctttgata atttgtaccc aggtggtcgc cagcatgccg ccgaacagga cgtacatcat
     1381 catcagcacg ccgaccagca ccaccgcaat gtgatagtta aggccaaaca gcagctcgat
     1441 cagtttacct gcgcctacca tctgagcgat aaggtaaagc gccaccacta ccagagaacc
     1501 acaggccgaa agaatacgaa tcggcccttg tttcagacga taagaggcca catcggcaga
     1561 ggtgtagcgt cccaggttac gcagacgttc ggcaatcaga aacagaatga ttggccagcc
     1621 caccaggaag cccaacgagt agatcagccc gtcatagccg gaggtaaaca ccagcgcgga
     1681 gatccccaag aatgaggcgg cggacatata gtccccggca atcgccagcc cgttctggaa
     1741 gccagtgata ttgccgcctg cggtgtagta gtcgctacga gaacgtacgc gttttgatgc
     1801 ccagtaggta atgccgagcg taaacacgac gaaaatcagg aacataataa tcgcctgcca
     1861 gttcgttggc tggcgctcta cggccccgct aatagcatcc gcggcgttag ctgcgaaagg
     1921 gagtgtggcg gcaagcgccg tcagaactct tttcatgatg cttgtacctc atgcaggact
     1981 tcattattaa gacggtcgaa ttcgccgttc gcccgccaga tgtagatacc ggtgagaaca
     2041 aaggagatca caatcactcc aacaccaatt ggaataccgc gtgtgacgct ggtgttcgga
     2101 ttcagcggcg tgcccagcca gccgggcgcg aaggcgatca gtaaaataaa gccgatataa
     2161 actgccagca taataatcga caggatggtg gcaaaccgtt gccgtttttc gactaactcc
     2221 ctgaaatgcg cattgtcttc tatccgctga taaatcgtac cattcatcac agattctcca
     2281 gaggtaatgt agggattgtt ttaattcccg ctcccttatg ggagaaggtt aacgctcggg
     2341 taacccttgc cgaatgtagg ccggataagg cgtttacgcc gcatccggca atcaatgcct
     2401 gatgcgacgc tgtcgcgtct tatcaggcct acaaaccgtt accgattcgc atccggcaat
     2461 tgtgggttac gatggcatcg cgatagcctg cttctcttca agcagcttct cgaccacgcc
     2521 aggatcggca agcgtcgagg tatcgcccag gttgctggta tcgcccgccg caattttgcg
     2581 cagaatacgg cgcataattt tgccggagcg ggttttaggc agggagtcgg tccagtgcag
     2641 cacgtctggc gtcgccagcg ggccaatctc tttacgcacc cagttgcgga cttctgcgta
     2701 cagttctggt gacggttcct ccccgtgatt aagcgtgaca taggcgtaga tcgcctgacc
     2761 tttaatattg tgcggaatac ctactacggc ggcttcggca atcttcggat gcgccaccag
     2821 cgccgactct atctctgccg tccccagacg gtgaccggag acgttcagca cgtcgtccac
     2881 acgcccggtg atccagtaat agccatcttc atcgcgacgc gcgccgtcgc cgctgaaata
     2941 catatttttg aaggtggaaa aataggtctg ctcaaaacgt tcgtgatcgc caaacagcgt
     3001 acgcgcctga ccaggccagg agtcggtgat caccaggcta ccttcggtag ccccttccag
     3061 cgggttacct tcgttatcga ccagcgccgg ttgcacgccg aagaacggac gtgttgccga
     3121 accggctttc agctcggtag cgccaggcag cggggtgatc atgaaaccgc cggtttcggt
     3181 ctgccaccag gtatcgacca ccggacattt ctcgttgccg atttttttcc agtaccactc
     3241 ccacgcttcc gggttaattg gctcgcccac ggaaccgaga atgcgtagcg acgaacggtc
     3301 ggtgccttca atcgctttat cgccttccgc catcagcgcg cggatcgccg tgggtgcggt
     3361 atagagaata ttgacctgat gcttgtccac cacctgcgcc atacgggcag gcgtcggcca
     3421 gttcggtacg ccttcaaaca tcagcgtggt cgcgccgcag gccagcgggc cgtacagcaa
     3481 ataactgtgt ccggtaaccc agcccacatc ggcggtgcac cagtagatat cacccggatg
     3541 ataatcaaag acatatttaa aggtcagcgc cgcgtacacc agataaccgc cggtagtgtg
     3601 cagtacgcct tttggttttc cggtagaacc ggaggtatag agaataaaca gcggatcttc
     3661 ggcgttcatc tcttccgcct ggtgctgatc gctggcttgc tcaaccaggt cgtgccacca
     3721 caggtcgcgc ccttcctgcc agtcgatgtt tccgccagta cgcttcagta ccaccacatg
     3781 ctctacgctg gtgacgttcg ggttttttag tgcgtcatca acgtttttct tcagcggaat
     3841 actacgcccg gcgcgcacgc cttcgtcgga agtgatcacc agtcgtgagt tggaatcaat
     3901 aatgcgcccg gcaacggctt ccggcgagaa gccgccgaaa atcaccgaat gcatcgcgcc
     3961 aatgcgggcg caggccagca tcgcaaccgc ggcttccggc accatcggca tataaatcgc
     4021 caccacatca ccttttttaa tgcccagctc gagcagggta ttggcgaagc ggcagacgtc
     4081 gcggtgcagc tctttatagc tgatatgttt gctctggctg gcgtcgtcgc cttcccagat
     4141 gatggcggtg cgatcgccgt tttcttgcag atggcggtca aggcagtttg ccgccagatt
     4201 cagcgtgccg tcctcgtacc atttaatgga cacattaccg ggggcaaagg aggtattttt
     4261 caccttctgg tacggtttga tccagtcgag aatttttccc tgttcgcccc agaaggtatc
     4321 aggtacgtta atagattgtt gatacatcgc ctcgtactgc tgagggttta tcaggcaacg
     4381 gtctgcgatg ttggcaggaa tggtgtgttt gtgaatttgg ctcatgcttt tgttctcctt
     4441 gtaggatgtt aataatatgt ggcataagcg ttaaatgtag gggtattggc agttttgttt
     4501 agtatttggg cgacagatca cgcaaaagta gaatggtgca aataaacggc agggtaattt
     4561 ttgaaggtca ccaacaaaag ttgattaatt ctttgaggaa catgcagtta tgcatgctgt
     4621 tgaaaaagag gaagatactg actaactcta aagtggtatt tcacatgcac ttacaattga
     4681 ttaaagacaa cattcacagt gtggttattt gttacacata ggggtgagca atgtcatgac
     4741 agtgtaggtg cggttactgt cgtgaaaaac aataaaaacc gccattgcaa caatggcgca
     4801 attcggatga agcccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat
     4861 tgattccttt tttctttttc acttctgttc acgctgaaca aacggcagct cccgcaaaac
     4921 ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca gcatcacgat caatatctct
     4981 cctggaaagc cacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc
     5041 tggtgatcct gtgggcgggg tatcccttct cgcgcgatta caacaagcct cgtggacatg
     5101 cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag
     5161 atggtccgct accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc
     5221 agaaagacgg cgaagatggc tacttccacg gtaaatgggc gcgcggcggc ccggaaatcg
     5281 tcaacaactt aggttgtgcc gactgccata acaccgcctc accagagttc gccaaaggca
     5341 aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac
     5401 cttttgagaa agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg
     5461 agtattactt cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag
     5521 tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt
     5581 cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc
     5641 acggtaaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag aacgccgaag
     5701 gcaaactcta caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg
     5761 cgaactgcca tacccaggat aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcaat
     5821 cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga
     5881 aagcggcgct ggatgcaggc gcgacggaag ccgaaatgaa gccgattcag gacgatatcc
     5941 gtcatgccca gtggcgttgg gatctggcga tcgcttccca cggtattcat atgcacgcac
     6001 cggaagaagg tctacggatg ctcggtacgg cgatggataa agcggcggat gcgcgcacca
     6061 aactggcgcg cctgctggcg accaaaggta tcacccatga aatccagatc ccggatatct
     6121 cgaccaaaga gaaagcccag caggccattg gcctgaacat ggaacaaatc aaggccgaga
     6181 agcaggactt catcaaaacg gtgatcccgc agtgggaaga gcaggcacgt aaaaacggtc
     6241 tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat
     6301 gagcgtatta cgttcgttat taactgctgg ggtgctggcg tcgggcctgt tgtggagcct
     6361 gaacggaatt accgctaccc ctgcggtgca ggcatctggt gatcgctacg aagttaccca
     6421 gcagcgtaac ccggatgccg cctgtctgga ctgtcataaa ccagataccg aaggtatgca
     6481 tggcaaacat gcttccgtta tcaacccgaa taacaaattg ccggtcacct gcaccaactg
     6541 tcacggccaa gcatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga
     6601 gccgatgtat aaggtcggag agcagaacag cgtctgtatg tcctgtcatc tgccggaaca
     6661 gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg
     6721 ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa
     6781 gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt
     6841 tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc
     6901 gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat
     6961 ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg
     7021 gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt
     7081 agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag
     7141 cactgcgatc atgtgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc
     7201 gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc
     7261 gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat
     7321 ttctgtcgta agaccaattt gcaggccggt aagcttcccg cgtgcgttga agcttgcccg
     7381 acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg
     7441 cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc
     7501 gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat
     7561 cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc
     7621 tggtgacgct ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagta
     7681 cgttgctgcg caccacgctg attgtcgggc cgggcgcggt gatcctcggt ctgttgatcc
     7741 tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta
     7801 cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta catggtggtg ctggtgctgt
     7861 ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc
     7921 tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacgc
     7981 tgatgctggt gttggcggtg ctgctggggg cttataccgg ctttctgctg tcggcgctga
     8041 aatcgtatcc gttcctcaat aacccgagcc tgccggtgct gctcctcttc tccggcatct
     8101 cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt
     8161 ccacggaagc gcactttgtt caccggatgg aaatccccgt ggtatggggt gaaatcttcc
     8221 tgctggtggc gttttttgtc ggtctggcgc tgggcgatga cggtaaagtt cgtgcgctgg
     8281 tggcggcatt aggtggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg
     8341 ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctgccg
     8401 tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt ttcattctct
     8461 acgccggaca gttgacggtg gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt
     8521 cctgaagtcg gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg
     8581 acggcctttg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc
     8641 attctggcgc agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc
     8701 atcagcgatt tctcggtcat ttatgtcgcc caacatagct acagcctgct gtcgtgggaa
     8761 ctcaaactgg cggcggtgtg gggcggtcat gaaggttcgc tgctgctttg ggtgctgctg
     8821 ctttccgcct ggagcgcgct gtttgcctgg cattatcggc agcaaagcga tccgctattt
     8881 ccgctgacgc tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtggtgctg
     8941 tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaatccg
     9001 atgctgcaac atcccggtct tatctttcat ccaccgctgc tttgccttgg ctatggcggt
     9061 ttgatggtag cggcgagcgt ggcgctggcg agtctactgc gcggcgagtt tgatgcggcc
     9121 agcgcccgaa tttgctggcg ctgggcgtta cctggctgga gtgcattaac ggcggggatc
     9181 atcctcggtt cctggtgggc ctactgcgaa ctcggctggg gcggctggtg gttctgggat
     9241 ccggtggaaa acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagtttg
     9301 tccctgacac gccagcgggg gattttccgc cactggtcgc tgttgctggc gatagttact
     9361 ctgatgctgt cgctgctggg caccttaatt gtccgttctg gcattctggt ttcggttcat
     9421 gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc actgattagc
     9481 cttgcgtctt tggctctgta tggctggcga gcgcgggacg gtggcccggc ggtgcgtttt
     9541 tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta
     9601 ctgatcgtgc tggtgggaac gctttatccg atgatttacg gcctgctggg ctggggacgc
     9661 ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac cgtttggtct gttgatgctg
     9721 gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc ggcgctggta
     9781 gctcatgctg gcgtgctgtt atttgccgcg gggatcgtgg tttccagtgt cagtcgtcag
     9841 gagatcagcc tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt
     9901 tttgagcgac tcgatctgca agccagaggc aattacacca gcgaaaaagc gatagtggcg
     9961 ctgtttgacc atcagcaacg cattggtgaa ttaacgccgg agcggcgttt ttatgaagca
    10021 cgccgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg
    10081 gtcatgggtg aaaaaaccag agcggatcgt tacgcttttc gtttgtatgt acaaagcggt
    10141 gtgcgctgga tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg
    10201 cgggggaaga agcgcgatga ataaagggct tctcacgcta ctgctgttat ttacctgttt
    10261 tgcgcgcgct caggttgtag acacctggca attcgccaat ccgcaacaac agcaacaggc
    10321 gttaaatatt gccagccagt tacgttgtcc gcagtgccag aatcaaaact tactcgaatc
    10381 caatgcgccg gtggcggtca gtatgcgcca tcaggtttat agcatggtgt cggaggggaa
    10441 gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa
    10501 cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt tgttattgct
    10561 gatggcactg atcctctggc gagtgagggc taagcgatga aacagcctca aataccggtg
    10621 aaaatgctga caacactcac gatattgatg gtattcctct gtgtcggcag ttatctgtta
    10681 agcccaaaat ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctacatcag
    10741 tttgccagcc agcaaacccc ggaagcgcag cttcaggcat tgcaggataa aatccgcgct
    10801 aatccacaaa acagcgaaca atgggcgtta ctgggcgagt actatctgtg gcaaaacgat
    10861 tacagcaatt cgctgctggc gtatcgtcag gcgttgcaac tacgtggtga gaacgctgaa
    10921 ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat gaccgcccag
    10981 actcgcgcaa tgatcgacaa agccctcgcg ctggactcta acgaaatcac cgctctgatg
    11041 ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa
    11101 aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat
    11161 atggcaaaac tgttgcagcg gagatcggat taatcatctg gtgatgaaga ttatgtcatt
    11221 gagctgcata aaaaataatc gaatgaacat atgccaaaaa taatcaccaa tcagtattat
    11281 tgcagattaa acaaataaaa atctttccat aataaatggt tattcattaa tcctgccata
    11341 aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca
    11401 taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa
    11461 ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg cgctccattg
    11521 aggaagttat tctgatgaaa aatataaaat tcagcctggc ctggcagatt ctgtttgcta
    11581 tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc cgcgactggc
    11641 tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc aaaatgattg
    11701 ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt gatgccaaac
    11761 agctcgggcg tattggcgcg aaaaccatta tttacttcga ggtgatcacc accgtcgcca
    11821 tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc
    11881 agctggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta caaagcagtt
    11941 cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg
    12001 cgaaaggcga aatgctgccg atcatctttt tctcggtgct gtttggtctg gggctttctt
    12061 ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca
    12121 tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt gcgctgattg
    12181 cggtgacggt ggctaacttt ggtttctcgt ctctgtggcc actggcgaaa ctggtgctgc
    12241 tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg gcacgtctgt
    12301 gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt ctggcgtact
    12361 ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa gcctacggag
    12421 caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt
    12481 cgacgctgta tcaaagtatt gccgctattt tcatcgctca gctgtatggc attgacctgt
    12541 ccatctggca ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa gggattgctg
    12601 gcgtggctgg cgtgtcgttt gtggtgttgc tggcaacgct gggtagcgta ggtattccgc
    12661 tggaaggtct ggcgtttatt gctggtgttg accgtatcct cgacatggcg cgtactgcgc
    12721 tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa cacaaatttg
    12781 accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat aaaactgcgg
    12841 atcaataatt gaagattgcc ggggatatcc cccggcaatg tgtggatgcc tgatgcgacg
    12901 cttgccgcgt cttatcaggc ctaccttaac cgttggttaa tttttcaaac tcttcacacc
    12961 cggtatcaaa cccttccata cagctcaggt tcaaccagtg cagcgccttt tgtttgttct
    13021 tctcgataaa accttcctca ccgtttaaga acatcattcc cgcccagtac tcggaataac
    13081 cggttcggga aattgcagag ctgcgtttga aataccaggt tgccttgtca tcgtcggcct
    13141 taatgcccac gccgttggcg taaatcaaac caagcagcat ttgggcatcg acggcggagt
    13201 cattctccag atcttccgag gcgttttcta acagcgaaat cgcctttgga taatccggtt
    13261 tacccgcttg agtatttacc agaatatgcg ccagcgttac ttcaccttct ttactgcccg
    13321 cttgcgccgc tttctctgca agaacttttg cttgtggata gtccagactg accggattgg
    13381 tgattttgat ctgcgccaac agcgcgcagg catcagcatc accgctgtcg gcggctttct
    13441 gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat
    13501 attgtgcacg tcggtccccg gcctctgctg cctttaaata ctggctgcca ggctcggagt
    13561 cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc gataattttt ttcattttga
    13621 taatacgatt gggtagggag taaccagtat aaagagttcg aatggataaa aaaacagcct
    13681 ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt
    13741 tcaacttgtt gtactcgtca atcacgtact gctcggcggc gcgctgatcg gagatcggct
    13801 cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt aagctttcgg
    13861 taaccagctc gttacaggca ccaatccacc actggtaggt catgtaaatc gcccctttgt
    13921 tcggacgatc gctgacctgc gcacgggtga taattttgcc tttacgcgag tgcactcaaa
    13981 ccaatgcctc atcttcaata cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc
    14041 caggttcatc agccagcgcc gccagtgccg cacagttacc ggtcatcgaa cggcaagagt
    14101 agtgaccaac ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg agtttgtcga
    14161 ttggcgctac ccagtcgcag gtgaagaact gcgccagacc gttcggggta tcaaacttct
    14221 ctttaaacag ataagaagtc ccctgatcgg catctgaagt atcgcggcaa ggccactgaa
    14281 tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg cacagatgac
    14341 gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat cggataaccc atacgggtgg
    14401 cgatttcact gatgatttgc cagtccgttt tcagatccca tttcggttca actgctttga
    14461 agaaacgctg gaagccacgg tcagccgcag taaacacgcc ttcatgctcg ccccacgacg
    14521 ttgacggcaa aatgacatcc gccgccgacg cggttttggt tataaagatg tcctgaacga
    14581 tgaccagttc cagatcttca aaggctttac gtactgccga cagctccgca tcagtttgca
    14641 gcggatcttc gcccataatg tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca
    14701 gctcgctgat gcgatagccg gtatgagcag gcaggctttc cacgccccag gctttggcga
    14761 atttttcgcg gttagccgga tctttcacgt actggtatcc cggatacgta tccggcagcg
    14821 cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt acacccgcat
    14881 gcggcttacc gaggttactg gtcagcatcg cgaggctggt cagagaacgc acggtttcca
    14941 cgccctggta gaactgggtt acacccatgc cccacaggat ggcggcgctt ttcgcctggg
    15001 catacatccg tgccgcctga cgaatctcac tggcgctgac accggtgata tcttcaaccg
    15061 actccggcgt gtagctttca acgattttac gatactcttc aaagccttct gtacgggaag
    15121 cgacgaacgc tttgtcgtac agattttctt caataatgac atggcccatc gcattcaaca
    15181 gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa
    15241 ttttgcgcgg atcgcagacg ataattttcg ccccgttacg tttagcgtta attacgtgat
    15301 tcgccacgat tgggtgggaa tccgccgggt tgtacccgaa aacgaacact aaatcggtat
    15361 tatcaatttc gttaatagca ttgctcattg cgccattacc gaccgattgg tgcagacctg
    15421 caaccgatgg gccgtgtcag acacgagcgc agcagtcaac gttattggta ccaataacgg
    15481 cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc cgtaccacgc gaggagccgg
    15541 tcgtctggat ggcatccgga ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt
    15601 aattcagtgc ctcatctcag gaaacaggtt cgagtttgcc gccacgctgg cgacggatca
    15661 tgggggtttt caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc cagccataat
    15721 aacccttcag acacagggta ccctggttgg ttttcccctg cgctgcctcc gcccggacga
    15781 ttttgccgtt atcgacgacc aggttgattt tgcaacctga tgcgcaatag gggcaaaccg
    15841 tgacgacttt tttcatcggt ctcgctccag ttaatcaaat cacgcatacg cgctctcgac
    15901 tacagtatgc atcttttatg ccacatttta tttggggtca ttccctgata ttacgggcac
    15961 tatttattca aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa
    16021 ataccgcgtg acagccatca cgcggcagac attttatttt ttctcgacga cgggagccgc
    16081 ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc
    16141 cagcatcagt aatgacatct cttcggcaag tactggcaac cgggcttcag tggcctgtaa
    16201 gcggctggtt aagccgcgct gataggcggc ctcggcagcg cgctgggtga agcgcgttgc
    16261 ttccacgtgt tcagcctgca tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt
    16321 gacggcaacg tcacgcaccg cgttcagtac tgactggttg taacgttcaa tcatcatgtt
    16381 gctggcggcg cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa acagcggcaa
    16441 tttcagaccc gggatgaagt tgaactggcg actggttttt ttgaataagg tatccaggtg
    16501 gatggcatca aggccgaaaa acgctttgat atcaaagctc ggatagaaca acgcccgcgc
    16561 ggaatccacc tgatctaatg acgcctgaac ataccagcgc atggcttgca gatccgggcg
    16621 tctggcgagc aactcataag agagtgtcgc cggaatgccg gtctggactc gtggtaatgc
    16681 cacaggtttg atctccggca tatcgctggc tcccgcgcca atcaatgcgc gcagagattc
    16741 tctcgtttcg gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg ccagaatctg
    16801 tgcccgcgcg ccgtggaaag gcacttgtgc ttccagaccg tgcgccactt tactttggtg
    16861 cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct aacagatcga gcatctgata
    16921 gctggcctgc atactgtaat aaagctgcgc tacgcccgtg gtcagcgata gctctactgc
    16981 tgcggtttct gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg
    17041 cacaccccac aaatcaagat ccagtccggc aaacaaacct actgtggctt ccgtatagta
    17101 cggcccgtcc atccccagtg cgggcgcatc catcgcataa gggcttaaaa agccgttcgc
    17161 cgagacacgt tggcggttaa gcatccctaa cgctgccacc tgcaactgtg aaccggcatc
    17221 taacaaatcg gcctgcgatt gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg
    17281 tgaaccactt agcgtccgtt ggatcagggc atccaactgc gggtcattga gttgtttcca
    17341 ccactgcgcc tgcggccagc cggagctggc aagatgaata tcatcggcca gtttgatttg
    17401 ttccggtttg agctgttgat gaggcgcaga atccttacgt accagggcac agccggaaat
    17461 cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac gattgatcat
    17521 tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc
    17581 aagcgggcga aaagctggca gacgtgttgc tcctgtttta ataaggtggg caggatggcc
    17641 tgcgaggtgg gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc ggttgccaga
    17701 ccggcagcgt atttctccag ggcgtcggca aactgacctg ctctgtccgg ctgtagtgcg
    17761 ttatccagcg cctgcgccga attccaggtg gcatcccagg cgtgaaggat atcgcggccc
    17821 tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct gtccagttga
    17881 cgctccagcg ccacgcgttg gcacatctct tcacaggcat taaacgccgc atgcagaccg
    17941 atacgaattt gcagataagt gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt
    18001 actttactta acatacccag cgcgccagcc agtttttgcg gcagcgtgcg cgcttcactt
    18061 tcaggccaga caaaggtgta aatcatcgcg gacaccacgg taccaatgat gatacccagg
    18121 gcgcgatcgc gaatttccac caggtcgtac actgggccaa aaacgttttc gagcgtggcg
    18181 agcgcgaagg tgaccaccat ctgtgtgccg atataagaag agcgttcaga gctggtggca
    18241 atccatgcgg ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac aatattgtcc
    18301 agccagggca tgaccagtag cgtgaataac agcgccagaa tcgcgccgca aaaggccccg
    18361 ccaaaacgca gcaccatctt ctggtacgac gaaccgacat ttggattagc gacgatcacg
    18421 caggtcagca tacaggtgtg aatgccttcc cagtccacgc cgctgtaaaa ggtgtaacag
    18481 atcaaacagg cgagcaatgt ttttaccgcg tagcgtatat agtctggatt ggtaaaagca
    18541 tcggcgacca ttgatggcgg tttggcggcg ggcgttggcg gcgtattcgg gttcatctgg
    18601 cccagttgta acaacgtctg gcagatattc tccaggttac attcccgtgc cgccaccgct
    18661 tcactttcac tgatccgcca gtcgctttgc cagcactgac cttcagcaac ggcatgctgc
    18721 agcttgttga tttctgaagc taatttttgc cgaaattcaa taattgcctg agaatcagca
    18781 aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt tgccacgcag
    18841 ctttgccacc atgcgctttg agttcgccag ttggcatcgt ccgcgagaca aaagacattc
    18901 agtttttgca gcgccagcgc ctctctttca atccgcgttt cgggtagcgg tgcgaggctg
    18961 tccgtcaggt ggctaatggc atcatcaagc cgatcattaa gcgcttgatg catttgcgta
    19021 atggcacgat tgggaaacca cagcacgccg attaacgtca tcagcaaggt tggatagagg
    19081 ccaacaacga tacaccacag cgttaagcgc acgactactt ccggatagtc gagcatggcg
    19141 gggaaggttt gcccgtaaat ggcgacagcg aaaaagacca gtcccagccg atgggtgcgc
    19201 atcaaaaaca tgcagcccat caggatcggc ccggcgatga tcaatcggat caacggttcg
    19261 ccgtatgacc atttgtagat caaaaacagg ctgccgatct ccagcaccgt ggcaaccaca
    19321 aacaagatcg ccacaaattt ggtgtaaaac gcgttcgact gaataccgat accgtaaaac
    19381 agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat cagaatcacc
    19441 agcaggcagc ctacccagag ttgcacgatc tgcggcacgc ggccgggtcg ccgctcgctt
    19501 aactcttcat gaaagaacgc cagcagcctg accaccggta atggcaggga gttgagcgcg
    19561 ctcattattg cggctcaaga ttagcgactg ccgaagcgcc gatgcggaac atttccgggt
    19621 caggtttatc gaccatgatt ttgaccggaa aacgctgggc aacgcggacc cagttaatag
    19681 aacgagacac ttttggcagg ccgcccagca ccaggccgcc gtcatccggt agcacgccgt
    19741 agccaatcga atccacttta ccctcgaagg ttttgccgct gtcactcatc aggcgaatcg
    19801 ttgcgggtgt acctgagcga atatttttca gatcggtttc gcggaagttg gcgatcacgt
    19861 accagtgacg agtgtcgatg agggtaaaaa tagggcgcat ggcagaagca aattgcccga
    19921 cggaggtttt gagggaaatg atccggccat caaatggcgc gcgaacggtc gtcatttcca
    19981 gatgtagttt ggtcagggca atatccgctt cgaccgccgc acgttgcgcc accagcgcat
    20041 cgacgccgct gacggcgctg gcggctgact gcgcctgtaa caatacggca ttaagatccg
    20101 cttctgcggc gcgctgcgcc gttcttgcac ggtcaacgtc ttctgctgag acaaaaccct
    20161 ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc ctgtttcgcg gcggcacggg
    20221 ctttttctac cgtggcatta accgagtcgg caccaaactg ttgggcgtca acgctacgct
    20281 gggtgagcat aatttgctta tccagcgccg cgagggaggc ttcggctttc gccaggttgg
    20341 cttcgtacgg gcgcgggtcg atgcggaaca gcaaatcgcc ctgtttcacc gcctggttgt
    20401 cggtgaccgc cagttctaca atgcgaccgc tgacttccgg caccacatca atggtatctg
    20461 ctgacgcgta agcgtcatta gttgatggcg cactgtctac gagccagata acgaaaacaa
    20521 gggcaaccag cgccaacgca accactaaca gagcagggaa tttactgcga ggtgcttttt
    20581 tcggcgtact ttccatgagc gcgtctcaaa tagattagaa aaatgccagc cagaacagca
    20641 tggcgtagag agcaaaaagg gcggtataaa taattccgac aaatgccaga ttgatatttg
    20701 ccc
//
LOCUS       DAEBXD010000070        20699 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEBXD010000070 DAEBXD010000000
VERSION     DAEBXD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.070"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            81..350
                     /locus_tag="KJL94_002691"
     CDS             81..350
                     /locus_tag="KJL94_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBD4410988.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KJL94_002692"
     CDS             344..709
                     /locus_tag="KJL94_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBD4410989.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KJL94_002693"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KJL94_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBD4410990.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KJL94_002694"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KJL94_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBD4410991.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KJL94_002695"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KJL94_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBD4410992.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KJL94_002696"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KJL94_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD4410993.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KJL94_002697"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KJL94_002697"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBD4410994.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KJL94_002698"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KJL94_002698"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBD4410995.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="KJL94_002699"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="KJL94_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KJL94_002700"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KJL94_002700"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBD4410996.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KJL94_002701"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KJL94_002701"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBD4410997.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="KJL94_002702"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="KJL94_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBD4410998.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="KJL94_002703"
     CDS             complement(16437..17195)
                     /locus_tag="KJL94_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBD4410999.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KJL94_002704"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KJL94_002704"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBD4411000.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KJL94_002705"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KJL94_002705"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBD4411001.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="KJL94_002706"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="KJL94_002706"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBD4411002.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="KJL94_002707"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="KJL94_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5692 c         5218 g         4899 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatac gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg caccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttgg
//
LOCUS       DAEBXD010000071        20521 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEBXD010000071 DAEBXD010000000
VERSION     DAEBXD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.071"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="KJL94_002708"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="KJL94_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBD4411003.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="KJL94_002709"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="KJL94_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBD4411004.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="KJL94_002710"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="KJL94_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBD4411005.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KJL94_002711"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KJL94_002711"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBD4411006.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KJL94_002712"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KJL94_002712"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBD4411007.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="KJL94_002713"
     CDS             4449..4814
                     /locus_tag="KJL94_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBD4411008.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="KJL94_002714"
     CDS             4964..5482
                     /locus_tag="KJL94_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBD4411009.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="KJL94_002715"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="KJL94_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="KJL94_002716"
     CDS             6713..7195
                     /locus_tag="KJL94_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBD4411010.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KJL94_002717"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KJL94_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBD4411011.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KJL94_002718"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KJL94_002718"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBD4411012.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KJL94_002719"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KJL94_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBD4411013.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KJL94_002720"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KJL94_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBD4411014.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KJL94_002721"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KJL94_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBD4411015.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="KJL94_002722"
     CDS             10939..11730
                     /locus_tag="KJL94_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBD4411016.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="KJL94_002723"
     CDS             11751..13037
                     /locus_tag="KJL94_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD4411017.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KJL94_002724"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KJL94_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBD4411018.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="KJL94_002725"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="KJL94_002725"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBD4411019.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="KJL94_002726"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="KJL94_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBD4411020.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="KJL94_002727"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="KJL94_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBD4411021.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="KJL94_002728"
     CDS             complement(16985..17275)
                     /locus_tag="KJL94_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBD4411022.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KJL94_002729"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KJL94_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBD4411023.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="KJL94_002730"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="KJL94_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBD4411024.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="KJL94_002731"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="KJL94_002731"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="KJL94_002732"
     CDS             19137..20378
                     /locus_tag="KJL94_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4411025.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5358 c         4815 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcgaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAEBXD010000072        20288 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEBXD010000072 DAEBXD010000000
VERSION     DAEBXD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.072"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="KJL94_002733"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="KJL94_002733"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBD4411026.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KJL94_002734"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KJL94_002734"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBD4411027.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="KJL94_002735"
     CDS             complement(1727..2998)
                     /locus_tag="KJL94_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411028.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="KJL94_002736"
     CDS             complement(3181..4386)
                     /locus_tag="KJL94_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411029.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLHSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="KJL94_002737"
     CDS             complement(4486..4842)
                     /locus_tag="KJL94_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBD4411030.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="KJL94_002738"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KJL94_002738"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KJL94_002739"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KJL94_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBD4411031.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="KJL94_002740"
     CDS             6772..9828
                     /locus_tag="KJL94_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD4411032.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="KJL94_002741"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="KJL94_002741"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBD4411033.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="KJL94_002742"
     CDS             10335..10523
                     /locus_tag="KJL94_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBD4411034.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="KJL94_002743"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="KJL94_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBD4411035.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="KJL94_002744"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="KJL94_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBD4411036.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="KJL94_002745"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="KJL94_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBD4411037.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="KJL94_002746"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="KJL94_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBD4411038.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="KJL94_002747"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="KJL94_002747"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBD4411039.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLIAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="KJL94_002748"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="KJL94_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBD4411040.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="KJL94_002749"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="KJL94_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBD4411041.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRIAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KJL94_002750"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KJL94_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBD4411042.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KJL94_002751"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KJL94_002751"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBD4411043.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5317 a         4854 c         5175 g         4942 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgtg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaata gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcatc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgata
//
LOCUS       DAEBXD010000073        20218 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEBXD010000073 DAEBXD010000000
VERSION     DAEBXD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20218
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.073"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="KJL94_002752"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="KJL94_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="KJL94_002753"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="KJL94_002753"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBD4411044.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="KJL94_002754"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="KJL94_002754"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBD4411045.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="KJL94_002755"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="KJL94_002755"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBD4411046.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KJL94_002756"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KJL94_002756"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBD4411047.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KJL94_002757"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KJL94_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBD4411048.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KJL94_002758"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KJL94_002758"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBD4411049.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KJL94_002759"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KJL94_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBD4411050.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KJL94_002760"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KJL94_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBD4411051.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KJL94_002761"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KJL94_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBD4411052.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KJL94_002762"
     CDS             complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KJL94_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBD4411053.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KJL94_002763"
     CDS             complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KJL94_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBD4411054.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KJL94_002764"
     CDS             complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KJL94_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBD4411055.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12654..13094)
                     /locus_tag="KJL94_002765"
     CDS             complement(12654..13094)
                     /locus_tag="KJL94_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBD4411056.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KJL94_002766"
     CDS             complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KJL94_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBD4411057.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KJL94_002767"
     CDS             complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KJL94_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBD4411058.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14036..15076
                     /locus_tag="KJL94_002768"
     CDS             14036..15076
                     /locus_tag="KJL94_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD4411059.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15112..16113)
                     /gene="add"
                     /locus_tag="KJL94_002769"
     CDS             complement(15112..16113)
                     /gene="add"
                     /locus_tag="KJL94_002769"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBD4411060.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KJL94_002770"
     CDS             complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KJL94_002770"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBD4411061.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KJL94_002771"
     CDS             complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KJL94_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBD4411062.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19166..20194
                     /gene="malI"
                     /locus_tag="KJL94_002772"
     CDS             19166..20194
                     /gene="malI"
                     /locus_tag="KJL94_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBD4411063.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5140 a         5120 c         5200 g         4758 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagccgcttc gacggcagct ttgcgcggat
     9481 caacctgttc ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     9661 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
     9721 gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg
     9781 ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
     9841 cccgttctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
     9901 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
     9961 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc
    10021 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    10081 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat
    10141 tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg
    10201 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca
    10261 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    10321 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    10381 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    10441 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    10501 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    10561 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    10621 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    10681 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    10741 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    10801 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    10861 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa
    10921 tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac
    10981 cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    11041 agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    11101 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    11161 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    11221 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    11281 gtttcagtgg aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg
    11341 tctgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    11401 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    11461 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    11521 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    11581 atgacacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    11641 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    11701 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    11761 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    11821 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    11881 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    11941 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    12001 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    12061 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    12121 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    12181 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    12241 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    12301 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    12361 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    12421 acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    12481 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    12541 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    12601 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    12661 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    12721 gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg
    12781 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    12841 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    12901 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    12961 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    13021 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    13081 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    13141 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    13201 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    13261 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    13321 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    13381 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    13441 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    13501 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    13561 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    13621 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    13681 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    13741 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    13801 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    13861 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    13921 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    13981 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    14041 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    14101 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    14161 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    14221 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    14281 agcggcgctt gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc
    14341 acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    14401 taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct
    14461 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    14521 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct
    14581 tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    14641 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    14701 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    14761 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    14821 cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    14881 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    14941 ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    15001 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    15061 gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc
    15121 ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc
    15181 attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt
    15241 atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg
    15301 aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt
    15361 ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat
    15421 cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg
    15481 aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca
    15541 gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc
    15601 atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc
    15661 ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac
    15721 accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac
    15781 aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag
    15841 gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga
    15901 ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt
    15961 ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt
    16021 atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg
    16081 gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa
    16141 gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta
    16201 cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt
    16261 tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac
    16321 cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg
    16381 cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga
    16441 tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat
    16501 ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat
    16561 gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata
    16621 aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa
    16681 tattgaaact tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc
    16741 agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc
    16801 gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt
    16861 tcccggtagg attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa
    16921 cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg
    16981 gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt
    17041 ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga
    17101 tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa
    17161 accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc
    17221 cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt
    17281 ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact
    17341 gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt
    17401 atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc
    17461 cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct
    17521 tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc
    17581 agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt
    17641 tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc
    17701 tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt
    17761 aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat
    17821 gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc
    17881 cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa
    17941 ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg
    18001 cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt
    18061 gatacatagc taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg
    18121 aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct
    18181 ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt
    18241 cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt
    18301 caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc
    18361 cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca
    18421 tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg
    18481 gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg
    18541 caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt
    18601 tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg
    18661 cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc
    18721 gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg
    18781 agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga
    18841 tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga
    18901 acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca
    18961 gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg
    19021 attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca
    19081 cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca
    19141 cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt
    19201 tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttaatg gcaaagggcg
    19261 aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg
    19321 caatcgacag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt tgatcgtccg
    19381 tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag ctctggaagc
    19441 acagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg
    19501 gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg ctgcaggaag
    19561 cagcgatgac ctgcggcgga tggcagaaga aaaagctatc ccggtgattt tcgcttcccg
    19621 tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg ctgcacagtt
    19681 gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg gagggcaaag
    19741 ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac taaaatttgg
    19801 cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc aagccgcgga
    19861 agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt gctataacga
    19921 aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa gcggggaaag
    19981 cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg atgcgacacc
    20041 aaccacactt gatgatatac ctgttacctg ggccagcacg ccagcgcggg aacttggtat
    20101 cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct
    20161 tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc tatacttg
//
LOCUS       DAEBXD010000074        20143 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEBXD010000074 DAEBXD010000000
VERSION     DAEBXD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20143
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.074"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            376..579
                     /locus_tag="KJL94_002773"
                     /pseudo
     CDS             376..579
                     /locus_tag="KJL94_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            598..5262
                     /locus_tag="KJL94_002774"
                     /pseudo
     CDS             598..5262
                     /locus_tag="KJL94_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5265..5645
                     /locus_tag="KJL94_002775"
     CDS             5265..5645
                     /locus_tag="KJL94_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411064.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(5700..5987)
                     /locus_tag="KJL94_002776"
     CDS             complement(5700..5987)
                     /locus_tag="KJL94_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBD4411065.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            6116..6625
                     /locus_tag="KJL94_002777"
     CDS             6116..6625
                     /locus_tag="KJL94_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBD4411066.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            6622..8040
                     /gene="phrB"
                     /locus_tag="KJL94_002778"
     CDS             6622..8040
                     /gene="phrB"
                     /locus_tag="KJL94_002778"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBD4411067.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(8190..9671)
                     /gene="dtpD"
                     /locus_tag="KJL94_002779"
     CDS             complement(8190..9671)
                     /gene="dtpD"
                     /locus_tag="KJL94_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBD4411068.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            9942..10685
                     /gene="ybgI"
                     /locus_tag="KJL94_002780"
     CDS             9942..10685
                     /gene="ybgI"
                     /locus_tag="KJL94_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBD4411069.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10708..11364
                     /gene="pxpB"
                     /locus_tag="KJL94_002781"
     CDS             10708..11364
                     /gene="pxpB"
                     /locus_tag="KJL94_002781"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBD4411070.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            11358..11804
                     /locus_tag="KJL94_002782"
     CDS             11358..11804
                     /locus_tag="KJL94_002782"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HBD4411071.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFCQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGD"
     gene            11835..12290
                     /locus_tag="KJL94_002783"
     CDS             11835..12290
                     /locus_tag="KJL94_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HBD4411072.1"
                     /translation="MEAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQ
                     SNRMGYRLQGQILKRITDRELLSHGLLPSVVQVPHNGQPIVLMNDAQTTGGYPRIACI
                     IEADMYHLAQIPLGQPIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            12280..13014
                     /gene="pxpA"
                     /locus_tag="KJL94_002784"
     CDS             12280..13014
                     /gene="pxpA"
                     /locus_tag="KJL94_002784"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBD4411073.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            13050..13841
                     /gene="nei"
                     /locus_tag="KJL94_002785"
     CDS             13050..13841
                     /gene="nei"
                     /locus_tag="KJL94_002785"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBD4411074.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(13838..14884)
                     /locus_tag="KJL94_002786"
     CDS             complement(13838..14884)
                     /locus_tag="KJL94_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBD4411075.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(15036..16112)
                     /locus_tag="KJL94_002787"
                     /pseudo
     CDS             complement(15036..16112)
                     /locus_tag="KJL94_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(16109..16840)
                     /locus_tag="KJL94_002788"
     CDS             complement(16109..16840)
                     /locus_tag="KJL94_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD4411076.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(16855..19314)
                     /locus_tag="KJL94_002789"
     CDS             complement(16855..19314)
                     /locus_tag="KJL94_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4411077.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(19365..19931)
                     /locus_tag="KJL94_002790"
     CDS             complement(19365..19931)
                     /locus_tag="KJL94_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4411078.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4966 a         4847 c         5645 g         4685 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 gctggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agccagtttc tccggggtaa cgagcaggtg
      421 taccagtgtc aggtggcatt tgtattgccg ggtgagcggg tcatgatggc cttcacctac
      481 gccagaacca caccgctgac cccggcagat atgacgcgct gggctgaaat taaaaagaat
      541 ctgcgcttca ggatgcggca ggaagtcagg acgaattaat catacaggga acgggacatg
      601 gaagaggcat tctgggcggc aagacagggt gatgccctgc tgcatacgtc gtttatggca
      661 gatgtgctgg gggcggtgct ggaggtggcc gcaaatgtcg ttattgatgc cctcattgtc
      721 ggcacaacct cgttactggc gacgtcagtg gggatcacga tggggtgcac ggccgtcgtg
      781 ctgaccggtt ttgtggcggg tgtggcgatg gtgtacaccg gcgtgacaga aaaggtcagc
      841 gaagcctgtt cagccctcgc aaatatgctt ttcccgcctc agattgaagg ttacatcctc
      901 accggctctg gtgacacatg gatcaacagc aaacctgccg cgcgggcagc ggcgactgcg
      961 gcatcgcggc aggacataga ggcgcaggaa gcgcaggcga aggcagaaca ggaagaagcg
     1021 cagcgtcagg aggatgccag aacgttccgg gatgtggccg gggaatatct ggaaatggcg
     1081 ggggccatag ccctggcggt taacccggtg acagggcctg tgctgatgtc ccgggctctg
     1141 cacgaccagc tcagtacgga agaagggcgt tctgaaatgg tggatggcgt gacacatttt
     1201 gtgtcggaac tatggcagcc cactgtggcc agtgcggccc cgggatccac cccgacccct
     1261 gacgataaaa tcgactgcca taaacatcct tcctcactga cgcagtttct cgcacaaaaa
     1321 aaagccgcct ttctggatga ccccctggga acggccctta acctgctgaa tccgggcggt
     1381 atgctggaaa ccgcctttca gggagtgaat gcggttattg gcagcatcag taatctgttc
     1441 aaaggcgatg acgagccgcc cgcagcggaa tatatcgcgg aaggcgcgcg ggatgtgcgt
     1501 atcaacagcc agccggcgac acgcagcggg gcacgctgta cctgcgaggc cagggtggtg
     1561 aatgagccgg ggaacggggc gtttgtgtca ccggatgtgc gcattggcgg tccgttactg
     1621 gtggtcaggg atatcagaag cggcaggtcg cagataaccc tggtggcaac cgtggcgctg
     1681 atgtttcttc gcccgggcaa aatgctttct aaaatcgcct gttttgctgc cagtgtcggg
     1741 atgagcatgc tgacgcagaa ggccaccagt gcccttccgc atccggtcaa tgccgccacc
     1801 ggggccaaat acctggccga tgacgatgac ttcgatttca gtctgccggg ccactttccg
     1861 ctggactggc agcgcgtgta cagcagtcgt gacgaacgta cggaaggaat gtttggtcag
     1921 ggctggagtg taatgtataa agtcagcctc gtgtgcacac cgggtagcgc agacgaaaac
     1981 tgcatgacct ttgtgtcggg catggggcga cgcttggata tggaagccgt actgccgggg
     2041 ggcgggtttt acagccccgg tgaagggctg gcggtaaggc gtggtgagca ggggcactgg
     2101 cttatcagca gcgatgacgg gcagtgtttt ctgtttgagg ctgacccgca ccacccgcag
     2161 cgccagcggc tgaaaatgct gggcgaccgt aacagcaact gtctgaatct gtactacgac
     2221 gaccggggcc gcattgtgga aatcagcggc gaacagcagc gtccgtgcat ccgtctgtat
     2281 tacgaactgg cggcgcatcc ccggcgggtg acacagattt atcagcattt ccctgagact
     2341 gcacctttac tgctgcgtcg ttacagctat gacgaggccg gccacctgaa cggcgtgtac
     2401 gacagcacgg ggcacctgct gcgtgagttt gcttacgacg aaaaccactg catgacgctg
     2461 caccgccagc cgggcggcga agggtattat taccagtggg gctggtatga aggtccggat
     2521 gacgcggcat ggcgggtgac ggggcaccac acggacagcg gtgaacagta ccggctggcg
     2581 tgggacctgg cgcagcgcag gctgtgcgtg acggacggac tggggcgcac acgttatcac
     2641 cagtgggatg cgcagaatca ggtgacggcg taccaggacg aagccggaca ggtgacgaca
     2701 ttccgctgga gcgacgaaga acgactgttg ctggggatga ccgaccctca gggcggcaaa
     2761 tggcgctatg tgtatgacct gcaggggcat atcacggaga cgcacgaccc gctggggcgg
     2821 gtggagcagg cgcagtggca cccggtgtgg caccagccgg aaacggaggt ggacgcggcg
     2881 ggaaacacct ggcgctgtgt gtacgatgaa cgcggcaacc ttcttgccgt gaccgacccg
     2941 ctgcagcaga acacccggta tcagtatgac caccacggct aggtggtgca gataacggat
     3001 gcgcgcgggg ggaataaata cctccagtgg aatgaagacg ggcagctcat gcggcacacg
     3061 gactgttccg gctcgcagac ggcgtggttc tacgacgaac gcacgaggct tatcagaatg
     3121 acggatgcgc agagccacag cacgcgctac gggtatgacg acagcggtca tctgacggag
     3181 gtgatactgg cggacggccg cacggtgaat taccagtcag acgcggcggg caggctggta
     3241 aagtacacca gcccgatggg gaggataacg cgctggcagc gggacgggca gggccgggtg
     3301 cgcagccgga cggacgcgat ggggcgcagg acggcattcg ggtacgacgc ttacgggcgt
     3361 ctggtcacgc tcacgaacga gaacggagaa cgctaccggt tccgccatga tgtgctggac
     3421 cgtctggcgg agcagataaa cccggacggc tgccgccagt cgtaccgcta caacgcgctg
     3481 aacgcggtca cggaagtggt gttcaccggg gaccggggcg gtgaaatccg ccaccggctg
     3541 gcgcgtgatg cggcgggccg gctcacggca aaagagacag ccgatggccg tacggaatac
     3601 gtccatgatg cggcagacca gctactggaa atccgtcgcc agcggcacga aactgacgcg
     3661 ccggaagtta tccgcttcag ctacgacaga ctggggcgga tgctgagcga ggagacggcg
     3721 cagggcatcc tgacccacca gtacgataaa ctgggcaacc ggactgccac cacctttccg
     3781 gacggcagaa cgcagcgcca tctgtactac ggcagcggcc atctccagca gataaatctg
     3841 gaccgggagg tcatcagcga gttcacgcgc gaccacctgc accgtgaagt gctcagaagc
     3901 caggggcgac tgagcacgcg gcagctttac gaccccggcg ggcggctgaa gcgcagggag
     3961 acatacagcg ggatgcgcgg ggtggtaccg gagacgttca cggaccggca gtacagctac
     4021 agtggcgagg atgaactgct gaagacgcgg cacagccggc ggggggaaac ggattacttc
     4081 tacgacccca cggggcgcat cacggcgtgt cgttcggagg atgaggggta tctggcgagc
     4141 tggcagtacg acgcggcggg aaacctgctg gggaggcgcg cgggggaaag ggccacggca
     4201 gaaaacagcg tggtgccgtt caaccggctg atgtcttacc gtggtgtgca ctaccgttat
     4261 gacgaatttg gccgtgcggt ggaaaaggag ggccggagcg gaacgcagag ttaccgttat
     4321 gatgcggaac accggatggt ggaagtgacg acagcgcggg ggacataccg gtatgtgtac
     4381 gatgcactgg ggaggcggac ggaaaaacag cacatcagcc ctgacggtaa gccgtacaac
     4441 cggacaaaat ttttatggga cggaatgcgg ctggcgcagg agagcaggcc ggagggaaca
     4501 ggcagcctgt acatctacag ggaccagggg agttatgaac cgctggcgcg ggtggataag
     4561 gcgggcaaag agggcccgaa ccggatactg tattttcaca cggatgtgaa cggcgcgccg
     4621 gaagagatga cggacagcga cgggaaaatc gtgtgggaga cggggtatca ggtctggggt
     4681 aacacgatac aggagaagga ccacggcggg gtggagcaga acctgcgcta ccagggacag
     4741 tacctcgaca gggaaacggg gctgcactac aatctgcaca ggtactatga tccggatgtc
     4801 gggcggttca tggtaactga cccgataggg ttgagaggcg gtttaaatct ctacagttat
     4861 gcacctaatc ctctaaaata tgctgatcca cttggattaa ctccatgtgc tgttagtaac
     4921 caaaaagcga atcgtttatt agatagtagt gaaaccaaag ttactgttag gtctagaagt
     4981 gacgctgaac aattgtttat ggatagatat ttaggtcata attataaaaa tatgacaggt
     5041 gagtcaggcc ccagcactaa gaacttaatg gaatatctta ctggaaataa aacaaaagca
     5101 ggttcatatc attgggatga tataaaagat ccttctgtaa caaaaccttc atatagagtg
     5161 tcaggtcatg gactaggaaa tcctgatggt gatttaccac atttacaagt tcatcaacat
     5221 ggtggaagtg ttagacatat atttttcccg tgggagacat aataatgaac aatctcaata
     5281 cattattaag tggaaaggtt ttgctcaaag aaattagtcc attaatgcct ggatatagaa
     5341 catggattga aatttcattg atcgatgaat taaaaccatc ttatcctttt agattagatg
     5401 aatatgctat gatagggcat tcaccttatg ccaatgaatg taataaagat gaagctaaat
     5461 ttaaactacg aatatcatca tttttagctt ctgacataga taatgaatac gatccatcat
     5521 atgattatgt tggaaaatat gaaattatcg cttcacttaa tgagttaaaa actaggttaa
     5581 gtactcttca tgtaaacttg gagcaattta taaattcatc ggaagatgat gagtatccat
     5641 tataataaga cagaataaaa agctaggaag gttttaaatc ttcccagctt ttttaatatt
     5701 taactttcgt tcagcacact aaacatccca tcccgcagtt cctgcacctc gtcactgtca
     5761 gcgatgatgg tcaggccccc ctccgaaatc ttcaccgtga caccccgtcc ggtatcaaat
     5821 ccggcctctt tcagccagtc gcccttaagg tgcaggctgg gatgttgtga ataataccgg
     5881 gctataccgg ttttgcggtc ctggtggcgg gctctgacat agccgacttt atagcggcgc
     5941 tgtgttttgg aaatggtgac ttctggcgta atatcgtgtt cagccatgat taactacctt
     6001 gattagttgc ttgcggggag cgcgctttcg taatcttgcc ctgctagcgg attaacttca
     6061 caaacatcat ttaccaacgc ctacacttac tcctgtatac cgttcaggag cagtaatgaa
     6121 tctacaacga tttgatgaca gcaccctaat ccgtatcttc gcccttcatg agttacatcg
     6181 actgaaagaa catggcttaa cgcgcggggc gcttctcgat tatcacagcc gctataaact
     6241 cgtctttctg gcccattctc agcccgagta ccgcaaactt ggcccgtttg tggctgatat
     6301 tcatcagtgg caaaatctgg atgattttta caaccagtac cgccaacgcg taattgtttt
     6361 gctttctcat cccgccaacc cgcgcgatca caccaacgtt ttgatgcacg ttcagggtta
     6421 ttttcgcccg catattgatt ccacagaacg ccagcagctg gctgcgctta tcgacagtta
     6481 tcgccgtggc gagcaaccac ttcttgcgcc gctgatgcgt atcaaacact atatggcgct
     6541 ttatcctgac gcctggcttt cagggcagcg ttatttcgaa ctttggccgc gtgtgattaa
     6601 cttgcgccat tcaggagttt tatgactacc catctggcct ggtttcgcca ggatttacgt
     6661 ctgcacgata atctcgcact ggctgccgcc tgccgcaatt cgtctgcacg cgtgctggcg
     6721 ctgtatatcg ctacaccacg ccagtgggag acgcataaca tgtcgccgcg tcaggctgaa
     6781 cttatcaatg ctcaactgaa tgggctacaa atcgcgctgg cggaaaaagg tattccttta
     6841 ttgttccgtg aagtggatga ctttgtcgcc agtgtcgaaa tagttaaaca ggtgtgcgcg
     6901 gaaaacagcg ttactcatct tttttataac tatcagtatg aggtgaatga gcgagcgcgg
     6961 gatgtggacg ttgaaagagc gctgcgtaac gtggtgtgtg aaggatttga tgacagcgtg
     7021 atcctgccgc cgggggcggt gatgaccggc aatcacgaga tgtacaaagt ctttacgcct
     7081 tttaagaatg cctggctgaa acggctgcgg gaagggatgc cggagtgcgt cgctgcacca
     7141 aaagttcgta gtagcggatc gataaagccc gcgccatcca ttacgctgaa ttatcctcgt
     7201 cagtctttcg atactgcgca tttcccggtg gaagaaaaag cggcgattgc gcaattacgc
     7261 cagttttgcg agaacggtgc tggagagtat gagcaacaac gagattttcc ggcagtggaa
     7321 ggcaccagcc gtttgtccgc cagcttggca acgggcgggt tatcgcctcg ccagtgtttg
     7381 catcgcttgt tggcggaaca gccgcaggcg ctggacggtg gggccggtag tgtctggctt
     7441 agtgagctga tctggcgcga gttctaccgt catctgatga cgtattaccc ctcgttgtgt
     7501 aaacatcgtc catttattgc ctggacggat cgtgtgcagt ggcaaagcaa tcccgcacat
     7561 ttacaggcct ggcagaaagg caaaacggga tacccgatta ttgatgccgc tatgcgtcag
     7621 cttaacagca ctggctggat gcataaccgg ctacggatga ttacagccag ttttctggtg
     7681 aaagatttgt tgatcgactg gcgcgaaggc gagcgatatt tcatgtcgca gctgattgat
     7741 ggtgatttgg cagctaataa cggtggctgg cagtgggcgg cttctacggg tactgatgct
     7801 gcgccgtatt ttcgtatttt caatccgata actcagggcg agaaatttga ccgtgagggc
     7861 gagtttattc gtcgatggtt accggagctg cgcgatgtac cagggaaagc ggtgcatgag
     7921 ccgtggaagt gggcgcagaa agcaggtgtg aagctggatt atccgcaacc gatagtcgag
     7981 cacaaagagg cgagagtaca aacgttggca gcgtatgagg cggcgcggaa ggggaaataa
     8041 cagacgcgtc aggcaatcga gcccagatgc cggatgcggc gtgaacgcct aatccagcct
     8101 acaaatttgt gcaaattcaa taaattgcag gaaatacgta ggcctgataa gacgcgtcaa
     8161 gcgtcgcatc aggcatcggt gctggcctat tatgactcca gcgccagcgc gcggtttctg
     8221 aatttcagcg cctgatacag ccaaatcatc agtaccagtc ctacacacgc cagtgcgccc
     8281 caggtaattt gatcaaatac ttcaatatat gcattgatgg agtagttgat cgccccggaa
     8341 gcatcaaacg aagcctgcga tgtctgatcg gcaatcacgc ccgccagata gttcgcgatc
     8401 gcgccagaaa gcagcatgta gatgccggtt aatacgccgg tcacgcccgg aatttcaatg
     8461 cgcgtaattt gcgacatggc aaccgggtcg ataaacagtt ccgcaaagcc catcaccgcg
     8521 aggcctaaca ccatcagtgg cagagaagag tgaccataca ttgcggacca gcgggcgctt
     8581 aaggtcagaa tgcagaatcc ggcgctcatc aggccaaggc caagagcaaa ttttccccag
     8641 atgcgcacgg tacgattacc tgcgacgctt tctttaacca cccacgccag gaacacaccg
     8701 cacagcataa ctgcgaaggc attaatcgac tggaacatcg cggtcggaac ggtataacca
     8761 aacatatcgc ggttaacgaa gcggtcgata taaaggctaa tcgagctacc gccctgttgt
     8821 gcgaaggccc agaacaacat actgaagaag gtgagcgtca caatcagccc cagctccttc
     8881 cgctgtttct ggttttctgc tttgcgataa atttttgcca gtacacccag accaataata
     8941 gtcgcgacaa ttaaggcgta taccgaccac tctttccaga acagcacggt aatcagtgca
     9001 ggcgttgcca ccagcagaac cagcagccat ccccagttcg gcaggagaaa gtttgtcgca
     9061 cgcagtactt ttttgttaac gccgcgggta tgagtgaaat gacgattgcc acataagaaa
     9121 atgaccagac ccgcgatcat gccaaccgcc gccaggccaa agcccatcgc ccagctgtac
     9181 tcttcctggg cgtaaccaca ggcgataggt gcgataatag atcccacgtt acccgccgca
     9241 tacatcagcg agaaaccgcc atcacgacgc ggatcggttg gctcatacag ctcgccgagc
     9301 agacagctta cgttagattt aaacaggcca tagccgcaga cgataatcgc cagggacaga
     9361 tagaggaatg acggatggat ctcactggca cccagcacca catgaccgat cgccatcaac
     9421 aacgccccca gcatcaccgc catgcgattg ccgagaactt tatccgccaa aaagccaccg
     9481 aggattggcg tgacatacac cagcgaacag taggcgctaa ataactcgta ggcgtgagta
     9541 tcgttgtatt ttagttgatt ggtgagatag agaatcagca gggcacgcat gccgtaaaag
     9601 ctaaaatatt cccagatttg cagcgcaacg acatagtaaa tagcgcgcgg ttgtgatgcg
     9661 tgtttattca taataattcc acaattgacg gcaacgtcac gtaatagttg cgcggcgtgc
     9721 ttgtgtttcc ttaaagttaa aactttgata cagatctgat tatttatgca atatgctgtc
     9781 tgattgcata aatatacatt agctgaagcg tgatgatata aacaatttgc gtcttttggc
     9841 gtcgggtttg tttcttaata tgtcaaaaga ttggctatcg acgaacaggc gatgtaacgc
     9901 tatgttaacg gtgtagctat cagaatacgg aaggcgcgaa gatgaaaaac accgaactgg
     9961 aacaactgat taacgaaaaa ctgaacagcg cggcgattag tgattacgcg ccgaatggtt
    10021 tgcaggtgga aggcaaagag acggtgcaaa aaattgttac cggtgtcacc gccagccagg
    10081 ctttgctcga tgaggcagtg cgtctgggcg cagatgcggt cattgtgcat catggctact
    10141 tctggaaagg agagtctccg gtcattcgcg gaatgaagcg taaccgttta aaaacgttgc
    10201 tggcgaatga tatcaacctg tatggctggc atttgccgct tgacgcacat cctgagctgg
    10261 gcaataacgc acagctggcg gcgttactgg ggatcacggt catgggcgaa attgagccgt
    10321 tggtgccgtg gggagagctg accatgcctg tgccgggaat ggaactagcc tcctggattg
    10381 aagcgcgtct gggacgtaag ccgttatggt gtggcgatac cggacctgag gtggtccagc
    10441 gcgtcgcctg gtgcacgggc ggcgggcaaa gttttatcga tagcgccgcg cgttttggcg
    10501 tggatgcttt tattactggc gaagtttctg aacagaccat tcattcagcc cgcgagcagg
    10561 gattgcattt ttatgctgca ggtcaccatg ccactgaacg cggtggtatt cgcgcattga
    10621 gcgagtggct gaatgaaaat accgatcttg atgtgacctt tattgatatt cctaatcctg
    10681 cataacgaat aatcagaggg atcgaaagtg caacgagcgc gttgttatct gataggtgaa
    10741 acggcggtag tgctggaact ggaaccgccg gtgacgctgg ctagccagaa acggatctgg
    10801 cgactggcgc agcgtctggt ggatatgccg aatgtggttg aagccattcc cggcatgaac
    10861 aatatcacgg tgattttgcg taatcctgag tcgctggcgc tggatgccat agagcgtttg
    10921 caacgctggt gggaggagag cgaggccctg gaacctgagt ctcgctttat tgaaattccg
    10981 gtggtttacg gtggtgcagg cggaccggat ttggcggtgg tcgcggcgta ttgcgggttg
    11041 agcgaaaaac aggttgttga attgcactcc tccgtggaat acgtggtctg gtttttaggt
    11101 tttcaaccgg gcttcccgta tctcgggagt ttgccggaac aactacacac gccacggcgc
    11161 gctgaaccgc gcttgctcgt tccggcaggt tctgtcggga tcggcgggcc gcagactggt
    11221 gtttatccgc tggcaacgcc gggtggctgg cagttgattg gtcatacctc actcagcctg
    11281 tttgatccgg cgcgtgacga acccatctta ttacgtccgg gagacagcgt gcgatttgta
    11341 ccgcagaagg agggaatatg ctgaagatta ttcgtgcggg catgtacacc actgtgcagg
    11401 atggcggtcg tcacggtttt tgccagtcgg gtatcagcca ctgtggcgca ctggatatgc
    11461 ctgcgctgcg cattgctaac ctgctggtgg gtaatgacgc caatgccccc gcgctggaga
    11521 tcacgctcgg tcagttaaca gttgagttcg aaactgatgg gtggtttgcc ctgacgggtg
    11581 caggttgcga agcgcggttg gatgataacg ccgtctggac cggctggcga ttgccgatga
    11641 aagcaggcca gcgtttaacg cttaaacgcc cgcagcacgg gatgcgcagt tatctggcgg
    11701 tcgcgggtgg tattgatgtt ccgccggtaa tggggtcatg cagcaccgat ctcaaagtgg
    11761 ggattggcgg gctggaaggc cgtttactga aggatggtga ctgactcccg attggcaaat
    11821 cgaagcgtga ttttatggaa gcgcagggcg ttaaacagct gctgtggggc aaccgcattc
    11881 gcgccttgcc ggggccggaa tatcatgagt tcgatcgcgc ctcgcaggat gcattctggc
    11941 gttcgccctg gcagcttagc tcgcaaagta accgaatggg ctatcgttta caggggcaaa
    12001 ttttaaaacg catcaccgat cgcgaactgt tatctcacgg tttgttaccg agcgtggtgc
    12061 aggtgccgca taacgggcag ccgattgtgt tgatgaacga cgcacagacc accggtggtt
    12121 atccacgtat tgcctgtatc attgaggctg atatgtacca tctggcgcaa attccgctcg
    12181 gtcagccgat tcattttgtc cagtgttcac tggaagaggc actgaaagcg cggcaagatc
    12241 agcaacgtta ttttgaacaa ttagcgtggc ggctgcacaa tgaaaattga cctgaacgcc
    12301 gatctgggcg aaggctgcgc cagcgacgca gagctattaa cgctggtttc ctctgccaat
    12361 attgcttgtg gatttcatgc aggcgatgcg caaaccatgc aggcttgcgt gcgtgaagca
    12421 ataaaaaatg gtgtcgcgat tggcgctcac ccgagttttc ccgacaggga aaattttggt
    12481 cgcagcgcca tgcagctgcc gccggaaacc gtttacgccc agacgctgta tcaaattggc
    12541 gcgctggcaa cgattgcccg tgcgcaaggc ggcgtaatgc gtcatgtcaa accgcacggc
    12601 atgttgtaca accaggcggc gaaagaagca caactggcag acgccatcgc cagagcggta
    12661 tacgcttgcg atccggcatt ggttctcgtt gggctggcag gaagcgagct gattcgtgca
    12721 ggcaagcaat atggcctgac aacgcgcgag gaagtgtttg ccgatcgcgg ttatcaggct
    12781 gacggctcgc tggtgccgcg aagtcagcca ggcgcgttga ttgaaaacga agaacaggcg
    12841 ttggcgcaaa cgctggagat ggtgcaacac ggcagagtca aaagtatcac cggcgaatgg
    12901 gcaacggtcg cggcgcaaac ggtctgcctg catggcgacg gcgagcacgc tctggcgttc
    12961 gcccgccgat tacgagctac atttgccaaa aaggggattg ttgtcgcagc ataaccccgc
    13021 ttaataaaga atgaaaaaag gatatcacca tgcctgaagg cccggagatc cgccgtgcag
    13081 cggataacct ggaggcggcg atcaaaggca aaccactaac tgatgtctgg tttgccttcc
    13141 cgcagttaaa aacttatcaa tcacaactta tcggtcaaca cgttacccat gtggaaacgc
    13201 gtggtaaggc gttgttaact cattttccca acggtttaac gctctacagc cataatcagc
    13261 tttacggcgt ctggcgcgtg gttgataccg gcgaagagcc gcagaccacg cgagtattgc
    13321 gggtaaaact gcaaacggct gacaaaacca ttctgcttta tagcgcctcg gatattgaga
    13381 tgttgaggcc ggaacaactg accacgcatc cgtttttaca acgcgttggt cccgatgtgc
    13441 tggatccgaa tctgacgccg gaggtggtaa aggaacggtt attgtcgccg cgctttcgta
    13501 accgtcagtt tgcaggatta ctgctcgatc aggcgtttct ggctgggctt ggcaattatt
    13561 tgcgggtgga gatcctctgg caggttgggt tgactggaaa tcataaagcg aaagatctca
    13621 atgcggcgca actggatgca ctcgcacacg cgttactgga gattcctcgg ttttcttacg
    13681 ctacgcgggg gcaggtggat gagaataagc atcatggggc gctgtttcgc tttaaggttt
    13741 ttcatcgtga tggcgaactg tgcgaacgct gtggcggcat cattgagaaa accacgttgt
    13801 cctcgcgccc gttttactgg tgtccaggct gccagcacta ggccgaccgc ttcggcgcat
    13861 aggttgaaat aaaccgcgca atggctggcc ccgtcagcaa aatactgaac aggcgcaggg
    13921 tttgcatcgc cataataagc gccatatcgg cattgctccc tgcggcgatg accgccacgg
    13981 tatcaagccc gccgggactg gtggcgaggt aggcagtcat aaaatcaata tgcataaacc
    14041 gggtcagtcc ccacgccata cctgcacaaa tagccagcag agcaaaaatc gacagcagga
    14101 tttgcggtag cgggcgtagt gcccgcagta agatttgttt atcgaaacca agaccaatcc
    14161 gccagccaat tgccatatac gccatcgcca gcagccattc cggtagttcg atggtgatga
    14221 gctgaccaga ctggagcact gcgcccgcca gcatcgggat gagcatcgtg ccggaaggaa
    14281 gacgcagcat gcatcccgcc gtaccggcaa cgactgcgag caaaatcgta agcaggagat
    14341 taatgctcac tggcgggaac cagacgattt gctggttaac cgcttcagcg ttatcaccca
    14401 gcatcatgcg ggtgaccaga acagcggccc ccgcgacgaa cagcactcgc agatattgca
    14461 taaacgccac caggcgaata tctgcgccgt aatcttgcgc catagcgacc attgccgctg
    14521 cgccgcctgg agaagaaccc caggcaccgg tatttccagg cagtgagcta tagcgcacca
    14581 ataaccaacc cacgatggcg ctggaaagca gcgtcaccag taaaatagca agcacgatcg
    14641 gccagttggc ggccagcgtg gtgagaatcg aaccagtgag gttttgcgca atcatgcagc
    14701 caagaatggc ctgcgcggcg agaaaagcgg agcggggaag ttgcagagta attccgcgca
    14761 tactgaagat gatcccggcg atcatcggcc caagtaatag cgccgccggg agatgtaccg
    14821 cgaggaaacc aatagaaagg agaagtgata gcacacataa cattccccac tgcaaaactg
    14881 gcatccttcg ctccctcatt attttctctt tggtgtatcg atacagcata aagaaagccg
    14941 ggctaggaaa aaataacagc aatcaaggaa aaggggaaaa tcagcaattt tctgaaagag
    15001 atgccctttc cggctgcaaa gggcatcatg gcaaatcagt tatagttaat tttaaaaagc
    15061 accactgtat cgaagggccc agcctcgatg ggggtacctg caatggacga aagttcagct
    15121 gtataggttt tttcgtagac caacatgtcg ctggtgaaat cggcgtattc agcatattta
    15181 ttgaaggtat aaggcgaggc gttttcatcc agcaaacgta gcttcagacc attaccaatc
    15241 aacaatgctt tatcttcttc aaccaacgtt tcttcggtat aaaaggagga actgacttta
    15301 aatccgtcgg tgcattggtc attttgtgat tttgtcgtct taatactgaa tgttttcgac
    15361 attgtgtggc gacgaatatc cagcaaatta aatttaccaa aatcaatcat ctgtgtttcc
    15421 ggtgaaatgg aaaaattgac gctgcaatcc agcacgcgaa tgttttccag accagtaata
    15481 tgatatttca ggttttgggc agtagggtct tcgttaacgc taccggcacc gtcaaattgc
    15541 acgacgatat agtcgctaag cgtactttga taatcatgcg gcggcatttc tcgtattttg
    15601 acgtataacc gcatgcgagc gagaaaggtt cgcgacatgt gaatattagc gggatcaccc
    15661 gagcaaattt tgttctgcca tcccatcagg ggtataaatg tcaatgttct tactgtcgat
    15721 acactggtta gtgtcgatgc ggctttttcc ctggttggcg tcataatcaa cgccattgta
    15781 agttactccg agttgataat aacgatcctg cacccccggg taggggttta cccatgcgta
    15841 gacatgttcg ctttcaaagt tgccgttggt attgttgtca caatagacgg gaattttaat
    15901 atcgtcagat tcccagatct tattttaata tcgtcagatt cccagatctt atcgccgagt
    15961 ttggcattgc ctggtacagc aaaaggttga atcgcttctg atttttctac cgagccaccg
    16021 gatgaaccaa agtagcagtt cagggcaagc gcagacttca acgacagaaa taacagacag
    16081 atgacgagca acactccctt gccagcactc atttcttcgc ctccacggct tgtaaatggc
    16141 attgtccgct gcagttgagc gtcgtcatgc gtaacgcgcc gtaatcatcc atataaccga
    16201 gataaaactg gctgccgcta taatttccgg tgtttgtgtt gaccgtacta aaaggcgcaa
    16261 ccatcacggt tttgaagccg gggagaacgc ctttttcatt tcgtcccagg taggcaatgg
    16321 tcagataata cgcggtagga tttttcagtg tcagttggtt gccctgctgg ctgacctgta
    16381 actgcagttc gaccttttct cctgctttct tgcgtaatgc agccggtcgc cagaacacct
    16441 tgatacgact ctgaatggcg acttgcagaa cggcatggtc gctgtttttt tccggggcgg
    16501 gaggaatttc acgcatattg tagaaaaata gcgtttcgcg atcgcccggt aattttgtgg
    16561 tcgaggcttg ctttactatc cgtacctgtg ttgttgcttt cggttccaga cgttgtatcg
    16621 gcggcagggc gaccagaaga tcatcgcttt tctccccttt ttcattttcg agccaggaat
    16681 aagcgaggta ggggagttta tcgctacgat tatccacccg cagactggta gctttatcat
    16741 tagcgttaaa cacaatacga gtgcgatcgg gttgtagtgc agcgtggctg cacagactgg
    16801 caaccaacaa caaaaggggt agtcctttca taaatgtcat tttcatttcc ttaattaatg
    16861 acaaggtaaa atcaggcgat ttgcggtgtc ttccatatgt tcaggaagat ggagcgagca
    16921 atgctggcta tcaccccaga ccacggtaaa cttttgattt tcagcgactc cgcttaacca
    16981 ggcatgtcct tcctcgccaa ccatccccac gctaatgccg ctgtcatcct ggcggatatc
    17041 cgctccgagg ggaggaaact gaccgctggc gttgcgaatg atgacgttaa cgtctttacc
    17101 ggaacgggaa gccagtgatt tgtaacctat cgcgccttca atccacgttt ctttgataac
    17161 gttttctgca actgttacgc cgtcgggtaa gtcattcatg ttcaccgcca cggtggaagg
    17221 ctggtaactg gaaatcaacg gcaccaccgc aatgccaaaa tggttggtgt agtcgagatt
    17281 gccctgaacc ggaatatctg ccacgccatc ggtgctgacc atcaggcgtg gttcattggt
    17341 ggagctgcgg cgatgaaacg ctgcaccata ttgggttgcg gtgaaagaac cgctccagct
    17401 gctgctgacg gaactgtaat cattggcggc ataggtacca gaaatatccc actcacccgc
    17461 tgaactcagg tgctgatagt taccgctcac ctgggctcca ttgtcaggac ggtcggattg
    17521 cagtccggca gacatgcccc agctattacg ttcatccagc gtatcgttcc acgacatgcg
    17581 gtgtgtggtg ctgtggctac tgttttgcat gtcatagcca acccgaccac cgttaccgaa
    17641 ggggagcgaa atcgacaggt aaatctggtt gtcgcgatct ttatcttcgt aatgggttgt
    17701 attgaatgac gtagaaatag agatatctct ccagtcacca atatcaacat taaaacctgc
    17761 cgtgatgttg gcggtcgtgg aggcgtctgc attccaccag gtttgatgta gcaggttggc
    17821 gtaaagattg aggtttagtg gggtaatcgg ttggcccaca gataagctga tcgtctgttt
    17881 ttcgtcctgc gcatcgctgt cgttgtattt gtgatccaga tagttggcgt agctgtgaaa
    17941 atgacgatcg gagaaacgat aagcggcgag ggaaatcgtg ctgttagtgg catccacttg
    18001 tttgctgtaa ttaaaacggt agcttaagcc ccgctcgtcc tgctgagtat caaaatggct
    18061 actggcccac gtgacatcaa acgacagcgc accaagccac agcatatttt gcccaatacc
    18121 cattgcggca gaatggtagt catcaccaga aagcagcagg ccgctgtaca gcgaggtgtt
    18181 tgacagcatc ccccaggaaa cttcattgct aaaaaaggtt tcattttcag tttgatgtga
    18241 catggagggc cgtggctgac ccgcggccag tttatagcga acctgtccct gacgagtcag
    18301 gaagggcgtc gatgctgctg aaacctggaa attgttcacc cgaccatctt cttccgtcac
    18361 tttgacatcc agtgtgccct gaacagactg attaaggtcg tcaatgataa atgggcctgg
    18421 tgggactttt ttctggtaaa tgacgcggcc tgattgactg atcgtcaccg tggcattggt
    18481 ctgtgcaata ccgctaattt gtggggcgta gccgcgtagt tcccatggca acattcgatc
    18541 gtcacttgcc agtgccgcgc cggtataaga aaaaccgtcg aaaatattgg gactaaaatc
    18601 cgtttcgccg agggttaatt ttgaacctaa ttgcggtaac gggcgaaaaa gataggtacg
    18661 tgatatttcg cctgactgtt catggttatc atcgctatca gtatgattca actgataatc
    18721 actgcgtaag cgccatgccc ccgtgttgat tccggctgtt ccataggcgt taagattagt
    18781 gctgctgctg ccatcctgtg ggcggtagct gctggcaaac aagttgtaat ccatcaggac
    18841 accggcgaca ccttctttcc atgtggaggg tggggtccag ttttctgagt gccacgccag
    18901 ccaggcttgc ggaatactaa tattcagttg ctgattggct tgatcgaaat tgaagagcat
    18961 ttcaggtcgg gagctaaaat cgacgcactg atttatcaat ggtaacgact gacggacttc
    19021 aggttttaag ccaaatttat cgaccagtaa atcattgatg cacggaatgg ttttatcgtc
    19081 atttttgtgc cagttaatct tttgctcgtt actgatttgg ttattattta ccgcaacgct
    19141 aacaaaatac tcaccgggag caatgactcc tttttccttt aataatgaaa tatcaatgcg
    19201 gtcgcgcatt gatttatcga gaacattcag attgaattcg accgccaatg cgcaaggcat
    19261 cgccacgacc aggcaggata caaaagagag tcgataaata ttcacggtgt ccatacctga
    19321 taaatatttt atgaaaggcg gcgatagcgc cgccaaagta atacttattt ataatccagc
    19381 acgtaggttg cattcgccgt aactgcgcct ggcgttacag gtgtagcctg atcaatttgt
    19441 tccatccagg caaagaaatt cagcgtttgt tcgctggagg tcggagccag gtcgatatct
    19501 ggagtagcgg tgcctaaaac gatattgctt tgatctttat tcattaaacg tacgccgacg
    19561 ccggtggctt cacccgtgct ggtgttgttg agaatcggat ctgcgccggt ggttttcgct
    19621 gagctatcaa aggtgactgc aactttagaa atcttgccac cggaaccgtt gctggagttt
    19681 tccagatcac agttgactaa gagcaggtcg acagcaacgg catcgctgtg atgtgactga
    19741 ttaatgtggc tggtggtcac ctgaccgagt tcaacctctt tatcaatatc atcctgatgt
    19801 atttcacacg gtgcttcaat aacttcacct ttaaagtgaa tttcaccgga tccttcatca
    19861 gccgcataaa caggtgctgt gaacagcaga gatacggcca gtgcggccaa tgttttttgt
    19921 cctttaaaca taactgagtc ctttaacaga aaagaaaaaa gtatatcggt gcaagaatat
    19981 cttatttgat tgtcgaatgt ttttagtttt tcttaaaaat attattaatt ttattaaatg
    20041 taaaagtaat taattgatgg tatgaattaa atgtttgatg aataaagtat tgggaattat
    20101 aggggtgcgc tatatttggt ggtttattca tgtaactgag agg
//
LOCUS       DAEBXD010000075        19375 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEBXD010000075 DAEBXD010000000
VERSION     DAEBXD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.075"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KJL94_002791"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KJL94_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBD4411079.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KJL94_002792"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KJL94_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBD4411080.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KJL94_002793"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KJL94_002793"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBD4411081.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KJL94_002794"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KJL94_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBD4411082.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KJL94_002795"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KJL94_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBD4411083.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KJL94_002796"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KJL94_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBD4411084.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KJL94_002797"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KJL94_002797"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBD4411085.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="KJL94_002798"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="KJL94_002798"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBD4411086.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="KJL94_002799"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="KJL94_002799"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBD4411087.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="KJL94_002800"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="KJL94_002800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBD4411088.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="KJL94_002801"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="KJL94_002801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBD4411089.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="KJL94_002802"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="KJL94_002802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBD4411090.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="KJL94_002803"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="KJL94_002803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBD4411091.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="KJL94_002804"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="KJL94_002804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBD4411092.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="KJL94_002805"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="KJL94_002805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBD4411093.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="KJL94_002806"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="KJL94_002806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBD4411094.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="KJL94_002807"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="KJL94_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBD4411095.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="KJL94_002808"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="KJL94_002808"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBD4411096.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAEBXD010000076        19260 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEBXD010000076 DAEBXD010000000
VERSION     DAEBXD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19260
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.076"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            31..330
                     /locus_tag="KJL94_002809"
     CDS             31..330
                     /locus_tag="KJL94_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD4411097.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            327..2075
                     /locus_tag="KJL94_002810"
     CDS             327..2075
                     /locus_tag="KJL94_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD4411098.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2364..2642
                     /locus_tag="KJL94_002811"
     CDS             2364..2642
                     /locus_tag="KJL94_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411099.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2639..2944
                     /locus_tag="KJL94_002812"
     CDS             2639..2944
                     /locus_tag="KJL94_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411100.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2954..3115
                     /locus_tag="KJL94_002813"
     CDS             2954..3115
                     /locus_tag="KJL94_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBD4411101.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3257..3481
                     /locus_tag="KJL94_002814"
     CDS             3257..3481
                     /locus_tag="KJL94_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411102.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3782..4939
                     /locus_tag="KJL94_002815"
                     /pseudo
     CDS             3782..4939
                     /locus_tag="KJL94_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4979..5551
                     /locus_tag="KJL94_002816"
     CDS             4979..5551
                     /locus_tag="KJL94_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBD4411103.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5553..6764
                     /locus_tag="KJL94_002817"
     CDS             5553..6764
                     /locus_tag="KJL94_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBD4411104.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6761..7099
                     /locus_tag="KJL94_002818"
     CDS             6761..7099
                     /locus_tag="KJL94_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBD4411105.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7096..7392
                     /locus_tag="KJL94_002819"
     CDS             7096..7392
                     /locus_tag="KJL94_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBD4411106.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7392..7832
                     /locus_tag="KJL94_002820"
     CDS             7392..7832
                     /locus_tag="KJL94_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD4411107.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7816..7998
                     /locus_tag="KJL94_002821"
     CDS             7816..7998
                     /locus_tag="KJL94_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411108.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8054..8344
                     /locus_tag="KJL94_002822"
     CDS             8054..8344
                     /locus_tag="KJL94_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBD4411109.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8364..10025
                     /locus_tag="KJL94_002823"
     CDS             8364..10025
                     /locus_tag="KJL94_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD4411110.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10039..10320
                     /locus_tag="KJL94_002824"
                     /pseudo
     CDS             10039..10320
                     /locus_tag="KJL94_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="KJL94_002825"
     CDS             complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="KJL94_002825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBD4411111.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="KJL94_002826"
     CDS             complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="KJL94_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBD4411112.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13417..14787)
                     /gene="purB"
                     /locus_tag="KJL94_002827"
     CDS             complement(13417..14787)
                     /gene="purB"
                     /locus_tag="KJL94_002827"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBD4411113.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="KJL94_002828"
     CDS             complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="KJL94_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBD4411114.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="KJL94_002829"
     CDS             complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="KJL94_002829"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBD4411115.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="KJL94_002830"
     CDS             complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="KJL94_002830"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBD4411116.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="KJL94_002831"
     CDS             complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="KJL94_002831"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBD4411117.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17924..19174
                     /gene="icd"
                     /locus_tag="KJL94_002832"
     CDS             17924..19174
                     /gene="icd"
                     /locus_tag="KJL94_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBD4411118.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5014 a         4727 c         5159 g         4360 t
ORIGIN      
        1 taaagaacaa cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc
       61 agtggcaaca ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc
      121 atggtaacga ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa
      181 tatgattgcg tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg
      241 ccccgcagta aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag
      301 atgcttaacg tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac
      361 gcggaataaa acacaaagac acagaggtaa ttatctttgc gggtagtgat gcctggtcgc
      421 acgcgaaaca atggcaggag caggatggcc ccgcatccgg cgataatgtg ccgcctgtgt
      481 ggcttgggcc aaatcagctt gccgaacttg atgcactgaa aattgttccg gatggaaaaa
      541 aacgcgtaag gctgtaccag gccggagaac tggatttggt ggagaccaaa aagattggtc
      601 agaagctggc ggcggcagat attcaggacg caaattttta ccccgaagga atgcacgtcc
      661 agaagtgtga aaactggcgg cgctatctga atgctgagcg tgaaaatatt gccgcagggc
      721 ttaccatgcc ggagcagaaa aatacgcaac tggcacaaat ggcagacagt gagcgcgcgc
      781 agctgcttgc cgagcgcttt gatggcgttt gtgtgcatca ggaaagtgaa atcgttcacg
      841 tatggcgcgg cggggtatgg tgtccggtca gcacaatgga acttagccgc gaaatggtgg
      901 cgatctattc agagcacagg gccactttca gcaagcgcgt aatcaataac gccgtggaag
      961 cgttaaaagt tattgccgaa cccatgggcg agccgtccgg cgatttgctg ccgttcgcca
     1021 atggtgcgct tgacctgaaa acgggggaat tttccccgca cacgccggag aactggatca
     1081 ccacgcacaa cggcattgag tacacgccac cagcaccagg ggagaacatc cgcgataacg
     1141 cgctaaactt tcataaatgg cttgagcacg cagccggaaa agaccagcgc aagatgatgc
     1201 gtatatgtgc cgcgctgtac atgattatgg cgaaccggta cgactggcag atgtttattg
     1261 aggccaccgg agacggcggg agcggtaaaa gtacattcac acacatagcc agccttctgg
     1321 cagggaaaca gaacacggta agcgctgaaa tgacatcgct tgatgatgct ggtggacgtg
     1381 cgcaggttgt cgggagtcgt cttatcgtcc tggctgacca gccgaaatat acaggcgaag
     1441 gaacgggcat caagaaaatc acgggcggtg accccgcgga aattaacccg aaatacgaaa
     1501 agcgtttcac ggctgtaatc agggcggtgg tgctggcgac caacaataac ccgatgatat
     1561 tcaccgaacg ggccggaggt gtggcacgtc gtcgcgtgat tttccgtttc gacaatatcg
     1621 ttagtgaggc cgaaaaagac agggagctac cggaaaagat tgcggctgaa atccctgtca
     1681 ttatccgccg attgctggcg aactttaccg actctgagaa agcacgggct ttactactgg
     1741 aacagcgtga cggtgatgaa gctctggcaa taaagcaaca gacggatccg gttattgagt
     1801 tttgccagtt cctgaatttt ctggaggaag cacgcggcct gatgatgggc ggcggtggcg
     1861 attcagtgaa gtacacgacc agaaacagcc tttaccgcgt ctatctggcg tttatggcgt
     1921 acgcaggcag gagcaaaccg ctaaacgtaa atgactttgg caaggctatg aagccagccg
     1981 cgaaagttta cggacatgaa tatattacgc ggaaagttaa gggagtaacg cagactaacg
     2041 caataacaac agacgagtgc gacgcgtttt tataattttt tgtaattgct gtctaccctg
     2101 tctacctgag taaagaaaaa tacatttaat tcagtgcatt aagttaggta gatagccttt
     2161 ttttactgtc tacccactat ctaccctctc tacctgattt tacctgaatc agacagggag
     2221 gtagacacgg ggtagatagt ggataaaagc actctacccc gctgaaagca gcgccattac
     2281 tggcatgagg gccactaagg tagataaggt agacaagggg aggcacaact caaaactttt
     2341 taaacgaggg ggtaaaaata aatatgcaca cttcagggaa atttaataaa tcattcaaaa
     2401 aacgcagaga cagaacagaa ccgaaatatc gcgcgttaga catgacagag cacgctttaa
     2461 aggtggcaat cagaacgata gaccgccacg cgggggaagg atacgcgaaa gcacatcccg
     2521 aactgataag cgcattcatg accacgacgg cggcaaattt tgccacgctg acagagcggg
     2581 agattgccga agcggaacag gtaacaacaa tcaacgttaa aaccggagag gtggaatcat
     2641 gagcgaacca aaatttggtg aaaaatttta caagcacaac ggacggatca cgattctgca
     2701 aataagcgca gctacaccag gctggtgggt tgaaacagac gaaggatcct cacccgtggc
     2761 atcatgggcg ctttgtgtgc ttagttaccc tgaccgcgat gtgtatcagg atattctgcc
     2821 tgtgatatcc actgacaaag gcatgaaacc cgtagacatc aaaaacatgg ggtttcagtg
     2881 cgtaatgctc actgaaaaaa tgatggaaga gatgaaaaag aacaatgccg gatcgcttca
     2941 ctgaggtggc aatatgacag cacaaattgc agcttacggg cggctggtgg gcgacccgct
     3001 ggtaaaacag agcagcaagg gcacaccgga gccgccaacg gagaactacg cagacctgag
     3061 aaatgcagta aatcgcactt acgcagacat ggggctgagc aaattagcag agtaggaata
     3121 tcagaaccac accacgcagc cggagcaatc cggctttttt tgcgtcattt gaaacggtat
     3181 ggagcactac tggcttttcg tcacggtccg gcatagttac tatctgaaac aaacagacac
     3241 aacagaggaa aacgcaatgc cgatgaaatt tgatgagata cttaaacagc gtgataaata
     3301 ccatgctgac aacatggaga cgatgaatat cactgattac cgcgcatttc tggaaacggg
     3361 ggcgctgatt gaaaaggatc atcatggttt tgttcgttgc gcgctatccg gtgaaatgct
     3421 ggcagtaaat cctgaacaga cagatgcatt gatagaattt ctgaaaggga tcagagactg
     3481 agcacccata cagcacatac agccggagca atccggcttt ttgttatttt ttgtaaatta
     3541 tttgttcgtg gttgttccac gttgttcact gagcggatcg gcatatttta cccgaactga
     3601 atcatgatta ttctcgcccg tggtgccagg acgctggggc cactttcccg cctgttaatg
     3661 tgctcgccaa tattcattac caggcgggaa aacgatcggt gcgattactg atttccttat
     3721 gaaaaacggt tgagtgtttg ccgcgtcctg gagttcctta cttaacccca ggactttttt
     3781 tatgcagaga ataatcgaat tacgccagca gaaaaccgcc attaaaaatc agatgcgcga
     3841 catgctggag aacgctgaaa aagaaaaccg cagtcttaac gatgctgagg gcacaaaatt
     3901 tgacgaatta cgcgctaaag ctgaatccct cgataaagac atttcccgcc ttgaagccat
     3961 tgcagacgaa gagcgcagca agccaggtaa aagcagccag accactgacc ccgcagaact
     4021 acgcaactac attctgaccg gtgaaacccg cgcattaagt acaggcgttc ccgctgatgg
     4081 tggttatacc gttatccctg aactgaacac cgaaatcatg cgaatgctgg cggatgaatc
     4141 caccatgcgt cgcatctgta ccgtgaagaa aatcagcagc aacgagttta agcagcttgt
     4201 ttccgctggc ggtgcgaccg ttaaccacgg tgaagagggt aaggcacgcg aacagaccag
     4261 caccccgcag attaacgagg tgagcattaa gctgtatccg gtctatgcgt acccgcgcac
     4321 cacacaggaa atcgtggatt tttccgatgt ggacatcctt tcatggctga cgggtgagat
     4381 tggcgacacc ttcacggaaa ccgaagaaag cgatctggtt gtgggcgacg gtgacaaaaa
     4441 agcaaaaggt tttttatccg taccccgtgc agagaagaac gacaaagagc gtgattttgg
     4501 tacgttgcag gtaattaaac cttccgaatc tctggcgtgg acatctgcgg acccgctgat
     4561 cgacctgaaa tttgcattac gtaaaaaata ccgcaaaaac gcggtctgag tggttaactc
     4621 cacgacggcg gcaaaacttc agaaggtgaa gaacgcgaac ggtgattata tctggcgcga
     4681 ccgtttacag acgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt
     4741 tatgcctgat aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca
     4801 cgaaacaggt gttcgcacca gaccggacaa cctgacagaa ccaggattca tcaaaatttt
     4861 cacgcaaaaa tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggcact
     4921 gccggaagac gacgattaac agcatacaga aggggctaaa aagccccttt agtgttttat
     4981 gggtgaaaaa attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcggagc
     5041 cggtacgctg acgggctacg ttgttcgctg ggataagctt tcagaactgt tatggggtga
     5101 gttttacgaa aaattccagc ggggggcgtt tactgaatgg cttgcggcgg gtaatgacgt
     5161 tcgcggcctg tatgagcatg accacagcat gttactgggg cgcacccgtt ccggcacgct
     5221 gaaactggaa gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagtac
     5281 agggcgcgac gttatcgaac tggttaagcg cggggacatc tcggggatga gctttggctt
     5341 tcgctcccgt aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt
     5401 ggcggaactg tacgaaatta ccgttacatc ggtaccggct taccccgatt ccggcgtgga
     5461 gctggcccgc cgttccctgt atgagcagca ccccgaaaaa atgccgcgtg cggataatcg
     5521 ccgctggtgg gcggatttag cgggggtgtg atatgtggcc tttcagacga aaaaaagagc
     5581 agcgcagcat gacgcttgat gaatttatgg cgctggccgg cacatcgaac acgggggcgg
     5641 gtgagtacgt atcatctggg acagcggaat cactccccgc cgtcatgaac gccgtcacgg
     5701 tcatctctga cgcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg
     5761 ggaaggaggc ccgcgaatgg cttgattctc atccggtcga tcacatcctc aacgagcgcc
     5821 cgaacgcgtg gcagacaccc taccagttta agcgaatgat gatccgctac tgcctgttaa
     5881 gtggtaatgc ttatgcggtt attcagtggg ggcgtgatgg ttttccggcg gctttacatc
     5941 cttacccgcc gcagtcggtg aacgtggagc agacaggtga tcataactgg cgctactgca
     6001 tcactgacgc ctacaccgga aacacccgca actatttacc gtgggaggtt ctccaccttc
     6061 gttactccac ggatgacggt tttatggggc gctcacctgt aaccatctgc cgcgaatcgc
     6121 tggggcttgg gctggcccaa caacgccacg gcgcgagcgt gatgcgtgat ggcatgatgg
     6181 cggcaggggt tatcacgtca ggcgaatggc tggacggcgt gaagggcaaa caggcattag
     6241 ccgcactgga acgctacaaa ggggccagaa acgccggaaa aacgcccatc cttgaagggg
     6301 ggatgagcta tcagcagctg ggcatgagta atcaggatgc cgaatggctg gcctcccgtc
     6361 gcttcaccat tgaagacatc gcccgaatgt tcaacgtctc gccgattttt ttgcaggaat
     6421 acagcaacag cacctacagc aacttcagcg aggcaagccg cgcatttctc accatgacga
     6481 tgcgcccgtg gctggcgaac tttgagcagc agataaaaaa cgccttgctg gtggcctcgc
     6541 ctgtacctgg tatccggtat caggtggagt ttgacagcgc ggacctgtta cgggccacac
     6601 ctggcgaacg ctttgccacc tatgagcgcg gcataaaatc cgacgttatg tgtccgaacg
     6661 aagcccgcga acgtgaaggg ctgtcaccgc gtgatggtgg tgatgagttc agccaggcat
     6721 ggaaacagga agtaaaaatc agcgagggag aaaaaccgga atgaacatag ggcgactgcg
     6781 tgacaggata acgattcaga ccctgaaaca gaccagggat ataaccggcg aaatactcga
     6841 aacgtgggag gacggtcaca cactctgggc aagcgtgaac atggtcagca gcaaggaggc
     6901 catttcatcg ggcgcagagc tggcgattgg taccgtaagg atctggatac ggtaccggaa
     6961 ggacatcaac gccaccagcc ggataaaggt cagtacgggg ccgctggcgg ggcgtgtact
     7021 gaatatcatc gggcagccgc tgccggatgc cgccaggaca cgccttgaaa ttctttgtcg
     7081 tgagggcgcg gaaaaatgac agaagaactt atcaccctgg aagaagtgaa actccattgc
     7141 cgcatcgatg gcgacgatga agaccagtta atcagcggat acattgccgc atcgcttgag
     7201 gcgtgccaga tacacatagg caggcgcttt gatgacgggc tggcgttcac gccagccata
     7261 aagattggct gcatgatgtt tatcgctcac ctgtatgaaa atcgccagat tgtcgcggat
     7321 aacgcaaaaa cacgcgtacc catgacgata ggcgcactct ggacggctta ccgtgatgtg
     7381 ggggggtact gatgccgtgg caaccattaa gacgatgcac agaaccaggc tgtaacaggc
     7441 gcgttaaatc cggcaagtgt gaggagcaca ggcgggcggc atggcgtgca gaggatgcca
     7501 gacgaggaca ccgccgcgcg cgtggatatt ccagacagtg ggacaaatac cgcgccatgt
     7561 atctgagtaa aaacccgtta tgcgtgcgat gccttgagaa ggggatatat acgcccgccg
     7621 tggtggtgga tcacattatc ccgattaatg gcggtgatga tgttctcttc tggcccgaat
     7681 ggaaccatca accattgtgc caggcgtgtc ataaccagaa aacgaaatgg cttgatccgg
     7741 caacaaaaag caagcgtgcc gcaggtggat ttcgtgaaga ggaagaacgg gctgctaacc
     7801 gcaataactg gatgtatgac gctgatgaat gagcgggaac aaaaccgcct tatccgtgga
     7861 cttataaggc agcgtgacgc atggaagaca caggaaacag ggcataaaga taaagcgtca
     7921 ggacgcgcag aacgcatcac agcgaagcga ttaaccgacc gtgaccgcga ggttatggaa
     7981 tgtttccgca atcgctgatg aggctgtatg acggggtggg gggcgttttc aggacaaacc
     8041 cgaccccgcc tacctggatg atatcgccgt gaagcagtgg cgggaaaaat cgcggcagct
     8101 tgcggaacag ggagacctga cccccgccga ctggagcaat ctggaactgt attgcgtcaa
     8161 ctactccatt taccggaaag ccgttgcaga ccttgcggcg cgcgggttca gcattgttaa
     8221 cagtcagggc ggcgagagca gaaaccccgc attaagcgca aaatccgacg ctgaaagagt
     8281 gatgataaaa atggcctcct tgctcggttt tgacccgata agccgccgta aaaatccacc
     8341 gtaaacagaa gaagaggacg agcttgaccg cctggaataa gtacgcagaa gacgtaaaaa
     8401 cgggcaaaat tccggcctgt aaacggttaa aacaggctgt taaacggtac ttttcggacc
     8461 ttaaaagccc cctttacacg ttcgatcgtg aggttgtgga gcggtttatt gccttttcca
     8521 gggtgtgccc gcacgtaaaa ggaccgatgc gtggcagacc cattgagctg gagccgtggc
     8581 agcagttcgc ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga cggcgcaaat
     8641 acacgagcgc ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc gccgcgattc
     8701 tggctaactg gtttctgatt atggagaacg gtcagcagga tatctacacc gcagcggtga
     8761 gccgtgacca ggcgcggatc gtgtttgata atgcgcgtca gatgtgcctt ttatcccgac
     8821 cgttacgcag gcgggtgaat attcaggcgc ataaggtgat acacccgaaa agcaacagcc
     8881 tgttaaagcc gctggcagca aaagcggcaa ccatcgaggg gacaaacccc agtctttcca
     8941 tcgtggacga ataccaccta caccccgata acggcgttta ttccgcgctt gagctgggga
     9001 tgggcgcacg tcccgaaggg ctgttatttg ccattaccac atcgggcagt aacgtcgttt
     9061 cagcctgtaa acagcactat gattattgct gccagatcct ggacggcgaa gaggtcaacg
     9121 attcaatttt tgtactgatt tacgaactgg acgacgaaaa cgaggttgat gatccggcga
     9181 tgtggataaa ggcgaaccct aatattgatg tttccgtgga tcgtgaaaaa ctggcctcaa
     9241 ccatccagaa agcgcggggt attccgtcgc agtgggtgga gatgatgaca aagcgattta
     9301 atatctggtg tcaggggact accccgtgga tgggtaatgg tgcatgggct gagtgtgccg
     9361 gaacgttcac ggaggaagat ttacacggtc aggagtgtta cgcggggctg gacttatcat
     9421 caaccagcga tatttccagc gtatgctatg cctttccggt cggtaaaacc attatgctga
     9481 tttcccgtca ttatctgccg gagttccagc tacagaaccc cgccaataaa aaccgcgctg
     9541 tctatcgcca gtgggctaaa gcgggctgga tacgcacaac gccaggtgac tgcattgatt
     9601 acgaccggat cagagatgac atcatgcagg atgcggagaa atttaatatc aggctggtgg
     9661 gctttgatac gtggaacgcc acgcatctca ggacgcaatt acagggggct ggttttgagg
     9721 tggagccgtt cccgcaaacc tacctcagat tcagtccggc ggcgaaatcg ttcgaagttt
     9781 ttgttaaccg cagggtgatt gtgcatcgtg gcgatccggt gttgtcctgg tcgatgagta
     9841 acgtcgtgat gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa aaatcaccga
     9901 acaagataga cccgagcgta gcggcgctga tggcgtttgg tacattccag gcagagcatg
     9961 aggattttgc ttttgatata agcgacagcc accgccagaa actggaagaa tttagcgggg
    10021 tgtaatgagg tcagcagcat gacagaagcc gaaatactgc gattaatccg ccgcgtctct
    10081 ggaatcagcc agtaggctga cgaacagacc acgcagccgg acagcgtgac agccgaaaat
    10141 tatgcgcgtg ttgttgctga ggtgatgcgc cgtgatggta tccagcttaa tgatgcggat
    10201 atgcgcgaca tacggatccg cgttctggaa atgctggcct acaatcgccg cgttgaactg
    10261 tatcgggaga aggagaaaat cacgtaccac tggaagaagc cggagcggtt acggcggtaa
    10321 ctcactgaga ttaaagcaaa gcgcaaaatt gcgctggcta catccctcac atctgaggac
    10381 tgttaaccag caggccggaa tatattcaga aattacgaaa ttttcgcagt ttaattactc
    10441 gtcagcttaa tcgctaaccc gctctgatat tttcggaaac ctcaatttga ggaagtcggc
    10501 gaggtaactg tttgatattc ccgatagcgc aaaaatgcgc ggtcgggcag aatgaccacc
    10561 acctcaatcg tgacggagag tgacaaccag tgacacaccg tgacaaaaag acggtttaac
    10621 cgttcattaa aaatcgacgt gattttgtgc tttccggatg acgtggccaa catcattttt
    10681 tagcgaaaaa ttctgattaa ggtcactggc tgaacggtca taatgaccac gcagaccatc
    10741 gaggcaacca tagcgatcat cgttacggtt ggagcttttc cgccattggt ggaaaaggtg
    10801 cacatctgga tcatcattac ggtgatcaag ggtatgtaaa acccaccagc ctgattaaag
    10861 gttaaccgga aaaaaaatcc ggtagccccg ttatggggat ccccatatcg acattaacgc
    10921 ccctcatgaa ttggtgcgct tccccctgga aagattaccc gcctgtatat ttcttgtgtc
    10981 tatttgttcc acgttgtttc atacagtgca ccgaacggtg tagttaatgg tgtagtcaat
    11041 ttgcgatttt tggcactttt tcaatgaaaa ttgtttgatc gaatcaatga cttgatgtag
    11101 tggtaaaagg acatatttat tcatctttcg gcgcagaatg ctggcgacca aaaatcacct
    11161 ccatccgcgc accgcccagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    11221 tgatttcgcg ggctaccgcc agccctacac cttgcccagg gcgtaaagta tcaacccgtt
    11281 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accggggcca tcatcctcga
    11341 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    11401 atttacaggc attatccagc acgttgccca tcacctcgac aaaatcgttc tgctcaccga
    11461 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    11521 tgttcagcgc tgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga
    11581 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    11641 ggctgatttg ctccagcatt accggctcag catcactgac gctcatcttt tcactacgca
    11701 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    11761 tgagcgtcgt acggtatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    11821 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    11881 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    11941 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    12001 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    12061 attttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    12121 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    12181 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    12241 agtcaggctg gatcattttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    12301 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    12361 cgacatgcaa cttattgttt tcccactgcg caagggtata gaacagattg ctctcgccac
    12421 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    12481 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca
    12541 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaggtagcc
    12601 ctggccgcga acggtggtaa tgacttcttg cggatattgt gcctgaattt ttttgcgcag
    12661 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    12721 tagcattaac gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat
    12781 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    12841 gagatcaacc tgaaacgggg ggagcgaaat gacctgtgaa gccagaccgc tattacgccg
    12901 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    12961 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    13021 cagaatcggc agtgaaacat cgttgctacg ccagcggcga atcagtgaca gaccgtcctc
    13081 gtcaggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    13141 ggcttctttg gcatcttctg cgtcatcgac ctgatgacca gcatcctgaa tctgaacttt
    13201 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    13261 ctgtcttaat tattaaaata gtgtaacgcg attatgtgtt tatgggggta aacattaaat
    13321 aaaccagcgg ggaggggagg taaagtgaaa aaagaaaaag tggataatct taataagcag
    13381 gccggacagc atcgccatcc ggcactgatt tgaggcttat ttcagctcat caaccatcgt
    13441 gatagctcga ccaatatagt tagccggcgt catcgctttc aggcgggctt tctcttcttc
    13501 tggcaacgcc agaccatcga taaactgctt catgccttcg gcgtcaacgc gcttaccgcg
    13561 agtaagctct ttcagcttct cgtacggttt ttcgatgcca tagcgacgca taactgtctg
    13621 gattggttct gccagcactt cccagttgtg atccagctca tccagcagat ggtcacggtt
    13681 cacttccagt ttgctcacgc ctttcagggt ggattgatac gcaatcagcg cataaccgat
    13741 acccacgccg aggttacgca gcacggtgga gtcggtcagg tcacgctgcc agcgggaaac
    13801 cggcagtttg cttgccagat gctgcaatac cgcgttggaa aggcccagat tcccttcgga
    13861 gttttcgaag tcgatcgggt taactttatg cggcatggtg gaagaaccaa tctcaccagc
    13921 aatggttttc tgtttgaagt ggttaagggc gatataaccc cagacgtcac ggtcaaagtc
    13981 gatcagaatg gtgttgaagc gcgcaacgca atcaaacagt tcggcaatgt agtcgtgcgg
    14041 ttcaatctgg gtagtgtacg ggttccattg aatacccagc gaggtgacga actcttcgct
    14101 gaactgatgc cagtcaactt ccgggtaagc ggcgatgtgg gcgttatagt taccgaccgc
    14161 gccgttgatt ttgccgagga tctccacctg gttaagctgg cggtgctggc gctccatacg
    14221 gtaggcgacg tttgccatct ctttaccgat ggttgacggc gtggctggct gaccgtgggt
    14281 acgagacagc agcgggatat cgcggtactg aacggcgaga tctttaatgc catcaatcag
    14341 ttgacgccag tacggcagga tcacttcatc acgcgcggtt ttcagcatta atgcgtggga
    14401 gaggttattg atatcttccg aagtacaggc aaagtggatg aattcagaaa ccgcgtgcag
    14461 ttccgggatc tccgccactt tttctttcag gaaatactca accgctttaa cgtcgtggtt
    14521 agtggtacgc tcgatggttt tgatgcgtgc ggcatcttct tcgctgaaat tggcgacaat
    14581 tgcatcaagg taaccgattg cgtcggcagc aaaagcagga acttccttga tcgctgcgtg
    14641 cgcggccagt ttttgcagcc aacgtacttc aacttgtaca cggaatttca gcaaaccata
    14701 ttcgctgaaa atcccgcgca gcgcgctgac tttatcgccg tagcgtccat cgacagggga
    14761 aacggcggtc agtgaggata attccataga tcacaactcc ggggttaaat gagcaagaat
    14821 ttgttttgcc tgagtggtca ggcgattacg agaaaacatc agttgtagac gtccgccgcc
    14881 gacctggtgc cagagcacgg cggcgcgaat gcctgccagc agggttgcgc gaactttcgc
    14941 ctgcacttgt gggctttgca gtacagcagg ggaaccggtg acctgaatgc gcgggccaag
    15001 cgggctgatc acatcaacat agatagcagc catcgcgctc atcagcgttt cggactgtaa
    15061 atcgaagtgt tcgagctggc gttgcaggcc gttgatccgg ttgcccagag tgtcgagcgc
    15121 gcctttcgct gaggagagtt tgcgctcaag caccatcaag ctgagtgtgt agcgggttaa
    15181 ttcggcgttt aagccctggc aactgctggc attgagcacg ccgagcaggg tttccagccc
    15241 gacgcgcagg ttggcttcgc taccgccaaa aaccgccagc gtcgagctgg ggttcatatc
    15301 aataatactg ttgagtgaga cgtgtagcgc atcggcatca caatgcccct ggtgagcgag
    15361 ttgttgcacc aggcgtgccg actgacaaat accggccagg gcgagggtga tgtcatagta
    15421 attctttgcc acgttcactg cttccttgtt ttaagtaaag ataataatca gaccggcagc
    15481 ggcagacgct gctcaataat accgccaccg aggcacactt caccgttata gaagacggca
    15541 gactggcccg gcgtcacggc ggcaaccggc tcatcgaaaa tcactttaat gcgatcgtcg
    15601 tccagcgcct tgacggtgca cggaatgtcg gtctggcgat agcgggtttt taccgtgcaa
    15661 cgcatagtgc cggtgaatgg ttcgcgatcg acccagtgca gctgctgggc aatcaacccg
    15721 acagacatca gccgcgggtg ttcatggccc tgagcgacaa ccagaatgtt gttttcgacg
    15781 tctttgtcca ccacatacca cggttcttcg gtgccttctt tggtgccacc gatacccaga
    15841 cctttacgct gaccgagagt gtgatacatc agcccctggt gctcaccaat ttcatcgcca
    15901 tcgacggtaa tgattttgcc cggttgcgcc gggagataac ggcccaggaa ctcgcggaat
    15961 ttacgttcgc caatgaagca aatgccggta gagtcttttt tcttcgcggt gaccagacca
    16021 agatcttcag caatcttacg cacctgcggt ttttccagtt cgcctaccgg gaacaggctt
    16081 tgcgcaatct gctcatggct gagcgtataa aggaagtagc tctggtcttt attgctgtcc
    16141 agaccacgca gcaggcggct cttgccatcg acatcggccc gacgtacgta atgaccggta
    16201 gcgatataat cggcacctaa atcttcggcg gcaaattcga ggaaggcttt aaatttgatc
    16261 tccttgttgc acagaatatc cggattcggc gtgcgaccgg ctttatattc ggcaaggaac
    16321 agttcgaaga cgttgtccca gtactcggca gcaaagttaa cggtgtgcag ttcaatgccg
    16381 agcttgtcgc agaccgcctg ggcatcagcc agatccgctg ccgctgtgca atattcctca
    16441 ccgtcgtctt cttcccagtt cttcataaac aggccttcga cctgatatcc ctgttgttgc
    16501 aacagccagg cagaaacgga ggaatcgaca ccgccggaca tgccgacgat tacttttttt
    16561 gcggtttcag acattggatc actcacgaca ttgaacttca aggcggcgta ttctatcacg
    16621 cagcctttta gatgacaccc tttgtaaaag gccagttaaa atcgccaatc atctccagcg
    16681 gataacgttg cccgctttga taacaacgaa tactttccgc caccagcggc gaacgaagat
    16741 ttgacgcctg taaaatttct tcggcgctga cccaacggca gcagtcgata tcgctgtcat
    16801 gaggctgggt cgggcatatt tgctcaagct caatggcaaa gaggaaacgc aaaaacggcg
    16861 ttttatctgg cgcaatccac tgatgcatac gaataaagtg ttgcggctgc gcgctgatgc
    16921 cggtttcttc ccacagttca cgggcggcgg cttccactaa ggtttcatcg gcttccagat
    16981 gcccggcagg ttggttccat aacgctttac cattaatcgt ctcttcaacg actaaaaatt
    17041 tgccttctgc gtgcaccacg caagcaacgg taacgtgcgg tttaaacatt ttgcatcctt
    17101 aatctgtcac ttctcgccat tcgccattgg caagattatc caaagagtaa tctcccatcg
    17161 catagcgaat cagtcgcagc gtggggaaac caacatgggc ggtcatgcgg cgcacctggc
    17221 gattacgtcc ttcatataag gtgatcttca gccagctggt gggaatactt ttgcgttcac
    17281 gaattggtgg attccgcggc cataaccacg cgggttcgtc aaccagctcc gcgcctgcgg
    17341 gcagggtagg gccatcattt aaggttacgc cattgcgcaa ggcttcaagt gcgtcttgtg
    17401 tgggaatacc ttccacctgc acataataga tttttccggt gcgtttaccc ggctgggtta
    17461 aacgcgcctg caacgcgccg ttattggtca gcaccagcaa cccttcgcta tcgcggtcaa
    17521 ggcgacctgc tgcataaacg ccctgaaccg ggatgaattc ttttaatgtt ttgcgtccgg
    17581 tttcatcggt gaactgcgga agaacatcgt agggtttatt gaagaggatc acacgcgtgg
    17641 gctggttttc atgtttacgc ctggtagaac gttgtgagct gaatcgctta acctggtgat
    17701 ttctaaaaga agttttttgc atggtatttt cagagattat gaattgccgc attatagcct
    17761 aataacgcgc atctttcatg acggcaaaca atagggtagt attgacaagt caattacaaa
    17821 tcattaacaa aaaattgctc taaagcatcc gtatcgcagg acgcaaacgc atatgcaacg
    17881 tggtggcaga cgagcaaacc agtagcgctc gaaggagagg tgaatggaaa gtaaagtagt
    17941 tgttccggca caaggcaaga agatcaccct gcaaaacggc aaactcaacg ttcctgaaaa
    18001 tccgattatc ccttacattg aaggtgatgg aatcggtgta gatgtaaccc cagccatgct
    18061 gaaagtggtc gacgctgcag tcgagaaagc ctataaaggc gagcgtaaaa tctcctggat
    18121 ggaaatttac accggtgaaa aatccacaca ggtttatggt caggatgtct ggctgcctgc
    18181 tgaaaccctt gatctgattc gtgaatatcg cgttgccatt aaaggcccgc tgaccactcc
    18241 ggttggtggc ggtattcgtt ctctcaacgt tgccctgcgt caggaactgg atctctacat
    18301 ctgcctgcgt ccggtacgtt actatcaggg cactccaagc ccggttaaac accctgaact
    18361 gaccgatatg gttatcttcc gtgaaaactc ggaagacatt tatgcgggta tcgaatggaa
    18421 agctgactct gccgacgccg agaaagtgat taaattcctg cgtgaagaga tgggcgtgaa
    18481 gaaaattcgc ttcccggaac attgcggtat cggtattaag ccgtgttctg aagaaggcac
    18541 caaacgtctg gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac
    18601 cctggtgcac aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta
    18661 ccagctggcg cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt
    18721 taaaaacccg aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct
    18781 gcaacagatc ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg
    18841 tgactacatt tctgacgctc tggcagcgca ggttggcggt atcggtatcg cccctggagc
    18901 aaacatcggt gacgaatgcg ccctgtttga agccacccac ggtactgcgc cgaaatacgc
    18961 cggtcaggac aaagtaaacc ctggctctat tattctctcc gctgagatga tgttacgcca
    19021 tatgggctgg actgaagccg cagacctgat tgttaaaggt atggaaggcg cgatcaacgc
    19081 caagaccgta acctatgact tcgagcgtct gatggaaggc gctaaactgc tgaaatgttc
    19141 agagtttggt gatgcgatca tcaagaatat gtaatcacta catgtgttaa atatttaaac
    19201 gggcgtataa cacgcccgtt gttttattta tgtggatatt attaatagca tatcgagcat
//
LOCUS       DAEBXD010000077        18024 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEBXD010000077 DAEBXD010000000
VERSION     DAEBXD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18024
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.077"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            155..586
                     /gene="osmC"
                     /locus_tag="KJL94_002833"
     CDS             155..586
                     /gene="osmC"
                     /locus_tag="KJL94_002833"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBD4411119.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(642..1568)
                     /locus_tag="KJL94_002834"
                     /pseudo
     CDS             complement(642..1568)
                     /locus_tag="KJL94_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1561..2547)
                     /locus_tag="KJL94_002835"
     CDS             complement(1561..2547)
                     /locus_tag="KJL94_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4411120.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            complement(2544..3440)
                     /locus_tag="KJL94_002836"
                     /pseudo
     CDS             complement(2544..3440)
                     /locus_tag="KJL94_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            complement(3454..3786)
                     /gene="ddpX"
                     /locus_tag="KJL94_002837"
                     /pseudo
     CDS             complement(3454..3786)
                     /gene="ddpX"
                     /locus_tag="KJL94_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            complement(4044..4661)
                     /locus_tag="KJL94_002838"
                     /pseudo
     CDS             complement(4044..4661)
                     /locus_tag="KJL94_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4586..5731)
                     /gene="dosC"
                     /locus_tag="KJL94_002839"
     CDS             complement(4586..5731)
                     /gene="dosC"
                     /locus_tag="KJL94_002839"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBD4411121.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            complement(5769..5810)
                     /locus_tag="KJL94_002840"
     CDS             complement(5769..5810)
                     /locus_tag="KJL94_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411122.1"
                     /translation="MVQLVDLARCVSF"
     gene            complement(6108..7427)
                     /locus_tag="KJL94_002841"
     CDS             complement(6108..7427)
                     /locus_tag="KJL94_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBD4411123.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(7558..9093)
                     /gene="gadC"
                     /locus_tag="KJL94_002842"
     CDS             complement(7558..9093)
                     /gene="gadC"
                     /locus_tag="KJL94_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBD4411124.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(9249..10649)
                     /locus_tag="KJL94_002843"
     CDS             complement(9249..10649)
                     /locus_tag="KJL94_002843"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4411125.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            complement(11010..13793)
                     /locus_tag="KJL94_002844"
     CDS             complement(11010..13793)
                     /locus_tag="KJL94_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD4411126.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(13850..16210)
                     /locus_tag="KJL94_002845"
     CDS             complement(13850..16210)
                     /locus_tag="KJL94_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD4411127.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(16248..17933)
                     /locus_tag="KJL94_002846"
                     /pseudo
     CDS             complement(16248..17933)
                     /locus_tag="KJL94_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         4680 a         4339 c         4266 g         4739 t
ORIGIN      
        1 cttgagatat atattcccgg ttatctattg tgggaattta atttaagtgc agaagtaata
       61 ttttcgccgt attttattcg gaatatcctg cttatcctcg tgctgtttct cacgtagtct
      121 ataatttcct ttttaagccc acaggagagc aacaatgaca atccataaga aaggtcaggc
      181 acactgggaa ggcgatatca aacgcgggaa gggaacagta tccaccgaga gcggcgtgct
      241 gaaccaacag ccgtatggat ttaacacgcg ttttgaaggc gaaaaaggaa ccaaccctga
      301 agaactgatt ggcgcagcgc atgccgcatg tttctcaatg gcgctttcat taatgctggg
      361 ggaagcggga ttcacgccaa catcaattga taccaccgcc gatgtgtcgc tggataaagt
      421 ggatgccggt tttgcgatta cgaaaatcgc actgaagagt gaagttgcgg tgccgggtat
      481 tgatgcctct acctttgacg gcatcatcca gaaagcaaaa gcaggatgcc cggtctctca
      541 ggtactgaaa gcggaaatta cgctggatta ccagttgaaa tcgtaaagca ttgccggatg
      601 acgcgtcagg cgcgtgaatg cctgatgcgt tgttagcatc tctacagcgc ccgccagcac
      661 cggagctcac gtccgtcctc ccttattgct aatgattgcc ggacttcaca tccgtgggtt
      721 gccagagggc aacgttcgta gaaaaaacag ccttgcggca acgtgcggtt tcccggcaga
      781 tccgttttac gtaatgctca ttcttcctcc agcggtttat caatggcggg gagggaatcc
      841 agcaataatc gggtgtatgg atgtgccgaa gcagtcaaca cctgttgcgc gtcgcccagt
      901 tctacaatct gcccgagata catcaccgcc acccgatcgc tcatatgacg tatcaccgag
      961 acattgtgtg aaatcagcac ataagtcagc ccgtgatttt cctgtagcgt taccagcaaa
     1021 ttgaggatct gcgcctgcac ggagatatcc agcgccgagg ttggctcatc aagcacaatc
     1081 acgtcaggct gcgaagagag tgctctggca atggcgatgc gttgccgctg cccgccggag
     1141 aacgcatgag gcaggcggtc gagatactcc ggacgaatac ccacctgcac agccaactcc
     1201 tctgccaacg ctcgccgctg ttgttcacta ctacgcttag ctatccagag cgattctgtg
     1261 atgatgcgcc acaccggtaa gcgcggatta agcgaagaga gcgggtcctg aaacaccatc
     1321 tgcataatgc gttgtgagcc tgaacggatg tactgcccgt ggctcggttg cagcatgccc
     1381 attaaaagct gtgcgagggt gcttttgccg cagcctgact cgccgacgat ccctaaggtt
     1441 tcaccacggc ggatctgtaa atcaatacca ttaatggcat gaacatgttc cgtagattta
     1501 ccaagccagt ttttacgggc cggaaaattg atatggacgt cgcgtaacgt taataacgtg
     1561 tcagacactt atgacctcct gctgcggata ccaacaggcg cagtgctggt tgttgtcacc
     1621 acacgctgtc agcgccggga cgttttcaca ctgtgcacct gccgcatagc aacgatcgcg
     1681 aaaagcgcag ccgtcaggca aatgggtgag gtttggtacc gtccctggaa tggcgggtaa
     1741 tggctggcgt ggtactccat gttctggtgc gcattgcagc aaaccaatgg tatacggatg
     1801 ccggggatga tggataacgt ctgccgtccc gccgctttca attacgcttc cggcatacat
     1861 cacgtaaacg ctatcgcaca gttgcgacac cacggccata tcatggctga tgaacagtac
     1921 cgcagttcca ctggcgcgtg ctttatgttt aagcaaacgc agtacctgca actgtactgt
     1981 gacgtccagc gccgtcgtcg gttcgccggc aataatcaat tgcggctcgc aggagaatgc
     2041 cagcgcaatc attacccgct ggcgcatacc acctgaaagc tcaaacggat agcgcgacat
     2101 aacttccacg gcatccggga tttgcatctc ttccagcagg gcaatcgctt tagctctggc
     2161 ttcccgacga cttattggtt gatgatggcg gattacgtcc atcatctgaa gacctattcg
     2221 acgtgtcgga ttaagggcgg tcatcggttc ctgaaagatc atcgccactc gtgcgccgcg
     2281 ccactgacga agctgttttt cccgagcgtt aagaacatct tctcccagca gtgaaatctg
     2341 cccccgatgt acgcaataac tgcccgtcgg tagcagacgc ataatcagca ttgcggtgac
     2401 tgatttacct gagccggatt ctcccaccag accgacaatt tcgccgcggt taatctgcaa
     2461 ggacacattg ttgagcgcgt gaacatcacc gttaaaaccg gggaaactca aatgcagctg
     2521 ttgaatgtcc agaacgggtt gggtcatgac tgctttcctc ctgctttcgg gtcaagcaga
     2581 tcgcgaatac catcaccaaa gagattaaac ccgacggagg taagcaaaat cgctgccccc
     2641 ggaaatgcgc aataccacca ctgatcgagt acatagttgc gaccattcgc taccatcgcg
     2701 ccccattcag cactcggttg ttgagcacct aggccaataa atcccaacgt ggcggccatt
     2761 aaaatcgcgc taccgatatc cagcgatgcc tgcacgatca gcggcggtag ggagttacgt
     2821 aaaatatgtc agttgatcag atgccaacga gacgcgccaa aggttttcgc cgcctgaaca
     2881 taggtatact ggcgtacaac taatgcttgc ccccgcgcca agcgcacata aaagggaatt
     2941 cgcacaatag caatcgccag catggcgtta aataaactcg gcccgagagc ggcagccagt
     3001 gccattgtca gtaccaacga aggaatcgac agcataatgt ccatgatgcg catgatgatg
     3061 gcgtctgcgc gtccgccaag cacaccggat agacatccga gtagcgaacc aatcataccc
     3121 gcaatggcga ccaccactaa tccggcgaga attgattgct gactgccgac cagtacgcgg
     3181 ctaaacagat cgcgtcccac ttcatcggtg ccaaaccagt gcgccgcaga aggcggcaaa
     3241 aggcgggcgg ttaaatcaat ggcgttggga tcatgcgtcg ccagccacgg tgaaaaaatc
     3301 atcatcagtc gcattaatac aatgatgact gcgccggtca cggtgagcgg actgcctttc
     3361 agcatccata ccagttttgc accgttaaat cgcgtttgtt tttgtgggcg tacggcggac
     3421 gtttcctcgc ttagcatcat tcaccacctc cacgtcatca cgaagcgtca ggtcgagcgc
     3481 cgtgccacgg ctgtgattag aaccgaccgt cacatcaaca acatattgcg ggtctgggca
     3541 ggcttgccac aacatcgcct gtgcttgttg tgggcgatac gcatcgtaaa tcaccagttg
     3601 taagcctgac agctgggcga tactgatact tttcgccagc gcggtaatcg catctttatg
     3661 tagcagacaa cgcgcttgct gataaatagc tttacctgtg atgttatcaa cgcaggcata
     3721 tttcaattcg atctccagat cagggaagat cacggctaaa tcaaccagtt cggtggtatc
     3781 cgacataaac ggttcctgta taagacaaaa attgctgcgc tttccgctta tgcagatctc
     3841 atgccatgcc gggataagcg gcagaatgct ggcttaaagt tatatttttt aacttttgat
     3901 caacatttgt gcagcgtagt gcagttttgg tgcaagaggg aaagttaagg aaggaatctc
     3961 ccggaatcgt agctgaaatc acagtattta agtgacagtg tcaccttaaa ggaaaacccg
     4021 cgagtgcggg cgagaggaat ttgtcagatt ttcagcggta acacgctgct catccagcct
     4081 ggaatttctt cggcgggaag ggggcgggaa aagaaatatc cctgaataac gcgacagtgg
     4141 atcttacgta gcatctcaaa ttgctctttg gtttcgaccc cttccgccac gacggttaaa
     4201 ttgaggcttt gcccaatgct ggtaatggct tcaagtaagg ccaggatgcg tttttccgtc
     4261 agacaacgat cgacaaagct tttgtcaatt ttgatttccg ttaccggaag actgactaag
     4321 cgggataatc cggaaaagcc cgtgccaaaa tcatctaccg ataaacccac gcccatatca
     4381 cgcaggatct gaatgcgctt aaagatttcg gtatcgtgtt ccatcatcat gctttccgtg
     4441 atttccaccg tcagctggtg gccgtcaata ccccaggctt gcattgcatc agacacctga
     4501 ttaggcagct gattactgcg aaagtgcagc gccgacaagt tcacggataa cgccgggata
     4561 tgaatattct ggctacgcca ttctgctaac tgacggcaag cttccgcgat gacccagcgc
     4621 ccaatatttt cgatttcacc aatctcttct gcgagaggaa tgagacttta cgcagaattt
     4681 catcgccagt gttatggccc cacgtatcgt tgatctcttt gaatttatca acgtcaataa
     4741 tcagcactga cagcggtgta ccggtccggt tggcatgggc aatttcgcgt ttgaagatag
     4801 tcggcaggaa acggcggtta agtaatttcg tcagtacatc cataccgact tcgtggcgcg
     4861 atacttcttc aaacaattca cgcagcaagg taataatctg cgatacggta tttcttatct
     4921 gtaatagaaa ttttacccgc aaacttctgt tattcaaaat cctggtgtta cgcatggttt
     4981 gattgaaaat accgtcgaaa tcctgaatca gacgggagat atggcccact tcggcaatgc
     5041 cgctaaaata atgtcgacct ttatggttaa accacaggcc aaaatcagcc tggcttaaag
     5101 gcaaactgct gcctaaatca gaatccagca ggactttata gataatatct atttcccatg
     5161 aaagtattga ggctatttgc cgttcttttt cttcttcggc gttttccagt aacgagaaga
     5221 tacgatagtt ttcatcttcc tttgaggcac tactgtcact aaaggtaaac gcgcgggtca
     5281 tcacttccat cgcgatatca atactgttaa tcgagaaatg gtagacctga agtttttctg
     5341 cggcggaata atccgaagag aagattaccg gatagaggat ttttttcagc acccgaaacc
     5401 ccatctcgac aatttctacc ggaattccta tgcgggcatg cacttccgcg acggtatgct
     5461 ggatttgtat tagcctttcg acatcgtcaa cctgggcaga aagcacgtta ataatccagc
     5521 gttccatcgc acttttcaac tgccgctcaa cttgttcatt actcaagaat tcttcggcat
     5581 gcgggtcgat gcggacaatt cgataaaact cgatactcag ataatgagca tgcgcaaggg
     5641 caatttctgc ggctttagca cgaatgagag gatctgcctg ttcgacaagt ccggtccact
     5701 catctttcat tcttttaaaa tacatctcca taattcacac ccttataagg ctgggaaatc
     5761 agacggaatc aaaacgaaac gcaacgtgcg agatcgacta actgcaccat attctcctga
     5821 aatatgaaga tatactgaaa agaaataagc gacttaagac agtttcaatc tacgctactg
     5881 ttcttcagaa gagtatagcc catcgtaatt atttttcggt gacagcgaat atcgtatggt
     5941 ttttcatatt catacatttt tattagggat ttatagctgt ttaactaagt gtggttaatt
     6001 gcgtggttaa tttgacttat gtaagcatga ttactagtgg gatatttcac aaggtgtaac
     6061 aagccggtgg gtaaagcacc ggcttgttac aaagtaagca tggtagttta actgccccaa
     6121 cgactttgca gatagctgac cgcttgttga gtctgcggtt tattcagata gtcctcacgg
     6181 aacaagatgg tgccgctaat ttctggtacc gcatcgttaa gatcgagctg ctttttcagt
     6241 tccggtacgc cgccgttaat catccagtct ggctctatct ttgaaggttc acccacttta
     6301 tagaaggcga taccgatata caggcgggtc ctggttggtt taacgacatc cgcccaccat
     6361 tttgccaaca cgtcataacg cgcggcactc cgtgagaacg gccagtaaat ttggggagca
     6421 atgtaatcca gcaatccttg ttccacccat cgacgggtgt ctgcgtagga ttcgtcatag
     6481 gctgccgcgc ctcgggtatc ggaaccgagc ggatcgtgtg atcggttacg ccacacgcct
     6541 gccgggctaa cgccaaattc gactcccggc ttaatgcttt taatggtgtg tgataccttt
     6601 gcaattaact gctgagtatt gttgcgccgc cagtctgctt ttgacgcaaa tgcgcctccg
     6661 tatttacggt acgtttcgtt atcatttagc cgtgaacccg gtgattcagt atagaaatag
     6721 tcgtcaaact gcacgccatc taccggatag cgggaaacca cttcagcgac tattgatgtg
     6781 atccagtcct gaacctcagg gatgcccggg tcgaggacaa agcgatcgcc agacgttctg
     6841 atccagtcac ggtgttgcac atagacgctc gccggttgtt gagacagagt gctattcagt
     6901 tccctgatag taccgggctt cgtattaacc gatacgcgat aggggttaaa ccaggcgtgt
     6961 actttcatcc cacgcttgtg ggcttcgtcg agcatgaatt gcagcggatc gtaacccgga
     7021 ttttcaccaa tcttaccggt cataagatcg gaccacggca aaattttcga tggccacagg
     7081 gcggtaccgt ccggcttgac ctggaaaaag accgtgttta tgccgagacg ttggagatga
     7141 tccagtttgt cgatcatcgc ctgttgttgt acacgggctc ggctggtggg attactaatg
     7201 ttaaccgagg aaaccggtgg ccagtcgagg cgagaaaccg tggccagcca gatgccacgc
     7261 atcggttgtg acgattgttg cgtcgtggct ggtggctttg aacctgctgg tggtgtcacc
     7321 atggactctg gaggcgtgct tttacaacta cacagtaaaa gtgccaatgc aaccagtatc
     7381 gctggtcttc taatcgttaa tttcttgttt cgggagcaga tatccatatg tgctggtttc
     7441 cggtaaacag atgtgcgctc attctcgtac ttatcccggt taagtcaata cgacagcaag
     7501 cacgaaaaag ggagcgatga attatcgctc ccttgtctta taaccattca gacatggtta
     7561 gtgtttcttg tcattcatca caatatagtg tggtgaacgt gcacgcgggt gcaggaagaa
     7621 gtgacctttt ggtgcgttct gactgttgat tggctccaga gtgacgccgg tattcgcttt
     7681 gcctttacga tcatgaacag catagagaat aaagggcagg gcaagtacca ccaggaaact
     7741 aacaaccagt aattcaacat acatatcggt agaatcaccc tggatgttat ccggcggcag
     7801 gaaggaaaca ataaacgcca taattgaagt cagcagaccg acaattgcca cgaccagttt
     7861 caccccttta ccaccaggga tattaaatgt gcgttttaag tcaggatgtt taagaaccaa
     7921 cacaatgtag ccaataaaca gcatgaaata agcacacaga taaatcacca ccgtcagcgc
     7981 cagtgcgatc aggaaggaca tgttgttacc gccaccggta ttggtgagga tgatcaacgc
     8041 gatagaagta atcaccagct gcgaaatgac cagcgttacc ggtacgccat ttttgttcat
     8101 tttagcgaat gccgctggca gcaggttttt ctgcgctgtg acatacatcc cgcgagaagg
     8161 accaacaatc caggaggcga tttccgccag aacacccagc aacagcagtg cggagatcac
     8221 gcgaaccgtc cactcaattt ccggtgccac atgggacatc agaacggtaa aggtttgcat
     8281 tacccctgcg gagaggttga tttcattacc cggaatgacc atcgcaatag acaaaccgcc
     8341 aacagagctt aagcagattg ccgccaccat cagcagtaac atagccagtg gatagtcgcg
     8401 cccggggttg ctcatttcat tgacgtgagt tgcggaagct tctacgccca tataactcaa
     8461 aatgaaggca acaaatacaa ccagtgtgcc cactttagag aagtcaggga agaaggtctt
     8521 cgaatccatt tcgatagcaa cgggggcacc ggagtgcagg taaatagccg ctaatgcgat
     8581 caaaataaat gcaggtaaca ggataccggc gaagaagcca actttagcaa ttcgcgccgt
     8641 gtatttcgtg ccaccaaact gcgttaatgc cagcgcccaa agaatgatga gtgctgcaat
     8701 agttttggta atggggtctt cattcagcgc tggccatttc aggatgtagg agagtgcccc
     8761 taacacgaaa tagagcatcg gaataaaacc aatggcgatt tgcagatagc caaatgagat
     8821 cgctgcaaat ccccatctcg gacccagagt atttgatacc caggcgaaga caccaccttc
     8881 ttcccagccg tcgacggtgg ccatttccgc agcacaaagt cccacgggaa taaaccataa
     8941 aatcccgcct aatagcagga agaagactaa tgaaaagccc gatgttgcga aggtagggta
     9001 ttcataaaca gccattacca tcgatgccgt tatggcaaag aatccgagta atgtgagctg
     9061 cttagcttta cctgtctgta ctgatgtagc catattatcc ccctaaaacg gtattcctgt
     9121 cggaaccgca cctgttttgt tctgaagcgt attcagaaca atattttccg ttgctaatgc
     9181 cagtgaacag actttggaaa ttatcccgaa acgggttcgt ttcgggacac cgttaccgtt
     9241 aaacgttatc aggtatgttt aaagctgttc tgctgggcaa taccctgcag tttcgggtga
     9301 tcgctgagat atttcaggga ggctttgtag tcttccagca acagttcagc aaagtccatt
     9361 tcgaagccgc gacgacacat aatgcgcatc accacgatgt cggtggcttc accgccgaga
     9421 gtgaaggccg gaacctgcca gccgcgcaga cgcagacgtt cagagaggtc gtacagggtg
     9481 tatcccggat cttcaccatc tttcagtttg aagcaaaccg ccgggatgcc ttcgtccggg
     9541 cgaccggtac agatgaactc atacggcccc agtttggcga tctcatccgc cagataggca
     9601 gcgacctggt aggaagcatt ctgtactttg gtatagcctt cacgaccaag gcgcaggaat
     9661 tcatagtact gtgcaatcac ctgacccgcc gggcgggaga agttgatggc aaaagtacca
     9721 atctgaccac cgaggtagtc aacgttgaac accagttcct gcggcagcgc ttcttcgtca
     9781 cgccagataa cccagccgca gcccagcgga gccagaccga atttatggcc tgaagcactg
     9841 atcgatttca cacgcggcag gcggaagtcc cagacgatat ccggggcgac gaacggtgcc
     9901 agaaagccac cgctggcggc gtcgatgtgc atgtcgatgt cgataccggt gtcggcctgg
     9961 aatttatcca gtgcatcgtg cagcggttgc gggaactcat agttaccggt gtaggtcacg
    10021 ccgaaagtcg gcaccacgcc gatggtattt tcgtcgcagg cttcaatcat gcgtttcggg
    10081 tccataaaca actgaccggg gcgcataggg atctcacgca gctccacatc ccagtagcgg
    10141 gcgaatttat gccagcagat ttgcaccgga ccgcacacca ggtttggttt atccgttggt
    10201 ttgcctgcag cttccatacg cttgcgccaa cgccatttca tcgccatccc gccgagcata
    10261 caggcctcgg aagaaccaat ggtgttggtg ccaacggcct gaccattttt cggcgcaggc
    10321 gcatgccaca gatcggcaac catgtttacg caacgcaggt cgatggctgc ggattgcgga
    10381 tattcttctt tgtcgatcca gtttttgtta atggataaat ccatcaactt gtggacgttt
    10441 tcgtcgtccc aggtctggca gaaagtggcc aggttctgac gagcgttgcc atcaagatat
    10501 aattcatcat tgataatctg aaatgcgaca tcgtcgcgca tttcgtgcag cggaaaacgt
    10561 tttgattctg cgatagtgga aatagacttc gcaccaaaac gtgaatcgag tagttccgac
    10621 cttaaatccg ttacttgctt cttatccatt ttaaactcct taaaatgatt ggatcgcatt
    10681 aaaaaagtag gatttatcga taaagtaagc aagtgcaaag gactcgtgtt taaataacaa
    10741 aatcctaatg ttatttatcg tgagatattg ttggaatgat attttttcat taaaaacaat
    10801 gtaatagaga attcttgggt ggtggaaagg tgttttatgt tgttattttt atgcgctttc
    10861 tgtgcctcct gaattatcac gtaaaaatca ggcattaaaa tatcactatt agtacttggt
    10921 tattattttg aacgcattta taaaattatt acataaaaat agcgaatatt gctaaaatcc
    10981 ccgccaacga tgtgttgacg gggctgttat tattttggca ataatactcc ggtataagta
    11041 tttaccggat gagaaagata ttgtttaacg gcagtgttaa cattctctac cgtcatttgt
    11101 ttcaacaatt gctcctgctc agtccatgct gcaggatcgt catattgaat aagactattt
    11161 ataatagtgt tcgctaattg ttgaacgcta cgctgttgga tatcgaggct gcgctgaacg
    11221 ttttgctggt attcgttcag ttcttgctca ctgatcccct tagtcaaacg cttaaccatc
    11281 acttcattcg ctaacgttaa ctgttcatca tgtcgttctg gttgacaagt aaaagccagc
    11341 aaatgactga tatctttagc ctgaggatca accgagaggc gagaagaaac gctgtatgcc
    11401 ccagatgcct gttcacgaat attaacgcgt agatcttttg ccagtgcgac gttaaaagca
    11461 tcgagcgcca tacgcgtcgc cagattaaca ggtgtccggg aatcataacg cttccactgt
    11521 gaaacctgtg ccacaggttc attttgttct tttacagtaa ccgatgcgtt gtccgtcgcg
    11581 cgagttaatg gtttacctgc ggctaatggc gaatcagagt gtttgattga gcctaagtaa
    11641 cgcgtaatta acgccacgag tttgtcttct gcgacattac cgacaatgac aaacgtgata
    11701 tccgctggag atgaaaacaa ctggcgatcg gcagccagcg catctgcggc agtaaactgt
    11761 gcaatctgat tttcttgcag taatttcgtg cggtcatcag catagcgcgt ctcatacatc
    11821 agctgggcga atttctccgc tggacgctgg tcgagcgttt tcaacgcctg aatttgagca
    11881 ttttgtagcg atgcccaaat attgtcgtta atcgtgctgt gggtgattcg ctggttaatc
    11941 aactggaaac caggttcagg gttattagtc cgcgcgctaa cggagagcaa cgtattcatg
    12001 ccactgactt tactgctcat ggttaccgag ttttccgcac tccagcgttt caggctggaa
    12061 gaggagagtt cgccaacgcc gcttccgcta actgctttat ttgccagcgc gataagtgat
    12121 ttttgctgcg cagggaaact taaatcgcct ttattcgata cggcaataat ttgtagcttt
    12181 tgctcttcac ccgcggattt tgccagaata accttggcac cattggaaag tgttaatgat
    12241 gtcagattct cagccagcgt ttctttgctg ctaatttccg cctgtgggtc agcgtctact
    12301 gttaacgata aatttctgcc tgggaagatg taagccgcca gctttttgtt ggcgtactct
    12361 ttttccagcg ccaggatagc cgcaggagac aatgcttttt tggcggcaag ctcattgttt
    12421 accatttgct cccaaaatgc gtcctggttc tttcttaact gctgccattt ttccgccaga
    12481 ctttgcacgg taatttgctg ccacagacgt ttcgaaagtt gatatgtctc ttccggcgac
    12541 aagaacggcg tattatttaa tgagctggat gccaggcgac tggtcagcat acgtaaatca
    12601 cgttcagctt gctgatcaac cgcatttttc agccaggtga ggcgggtaga tttgacatca
    12661 tcgagttctt cagcagaaaa accatgctga tcaatggttg ccaactctgc cattaatgca
    12721 ttcgcagcat cctgcatatt atcgtctcgt gcatttacac ggaaaaacag cgactgataa
    12781 tcgggtgcaa ttttaacgct gcgcgcagtg ccgccagaaa tagtcttcaa ctcgcccgac
    12841 tgtatgcgtt cctgcagacg ttgattgaac agctgaacta acatgctcca ttcagcttgt
    12901 tcgacaaagc tttgctcatc gttcacttgt accattggca ggcgataata gagtgcgatg
    12961 ccgttcaccc ggttttcttt atcattaatg atattaaagc gcaggtggtt ttcggctttt
    13021 gtcggccaga cgcgattttc agctgctttg ttagccggaa gcttacttaa attatccttt
    13081 atcagcgcca gcgcttcttt actgtcgata tcgccgacca cgataaaggt catattattt
    13141 ggttgatacc agcgttgata aaattggcgc aattgtgccg gcgtgaccgt ggcgacagta
    13201 tccatcaggc cgataggttc acggtctaaa ttacgggtat tagccagcag gaaagggcgg
    13261 cgcgcctgag aggtgcgcca tttcgcatcc tgatgggcac gccattcctc agtaattacg
    13321 ccacgttcag cgtctacttc gagtttttca aaggttgcgg cattactcca ttcactgaag
    13381 attgccatca cttgttgcag attttgtttc tgcgtagtcg gcaaactcac ctgatacacc
    13441 gtttcgtcat agctgatata ggcattaaca tcgcgaccaa aacgcaggcc cattgactca
    13501 aatgtttcga tgactttatt acccggccat gtttttgtgc cgttaaacat catatgttct
    13561 acaaaatgag ccacgccgag ctcattgtct tcttcctgca atgaaccggt atgaatttgc
    13621 agccataaat ttacctgatc ctttggatga gcatgcggat aaatcatata tcgcaagtca
    13681 ttgtccagtt gcccggtaat taacttttca tcctgcggta aggcggcggc aatcagccgc
    13741 ccggggagca acagagttgc cactaacgtc agtaagaaac agaggtttct cataattatc
    13801 tccatgcgaa aaccgggcga atttacccgg ttaagtaaaa tccgaactat taaaatttca
    13861 tgctgacatc aagccagaaa gtacggccac tggcgtaggt cgccacaccg gtgttcgttg
    13921 tatcaacagc ggttttgcta tccagtacat tgagaatatc ggcactgatc gtcaggtttt
    13981 gttgtttcag aaattgtggc gtccaggaca agcgggtgtc ccaggtcagg ctgctatcca
    14041 gtttttcgtc aacatactgc ttgtaatctg aatattcgct gatgtattgc gcatttgtct
    14101 taccaaggat aatgcgagct ttacgcgctt cttgccaggc cagcgtattc gcccacacta
    14161 acccgctcgg ttgatgcgtg aaatccatgt ttaaggagat ctttaatggg ttattaaaat
    14221 ctgccactgg aacgctatcg taagagacca gattaccgtt ataaaccacc tggttatcac
    14281 cggtattgct ctcctcataa ccattgttta acgacaagtt gcccttgctc ttgatatagc
    14341 taaagacaat ttgcgggtta atatctacct ggcggatatg caggggttcg gccagttcaa
    14401 aactgaggtt aaacgaatgc gttttggttt tgccatcgtt gttatattca gtaatggtgg
    14461 ttttagtcgc gctgtcggta cgactgcttt tgctgatttg atcatgcgct tcacttgcgc
    14521 gtgcaataac gttcttatcg attttttgct gcaatcccat tgccagttca tcgttataag
    14581 gcgttttcaa attctgataa cgcgtcaggg ttttattacc tgataccgat tccgtccagc
    14641 tattgcggat atcacgtaat cccatatcaa gaatattccc gccatagtaa cggttataac
    14701 cggcggtaat cattgaggtt tgatcagcaa aaatatccca ttccgtcata aagcgcgggg
    14761 agatattgtg gtttgacaga tagttgtcat agtcataacg cacaccgggc attaacgaca
    14821 cattccgcca gctaatgtga tccgccatat acagcgtgta gttgtcaatt cccaggctgc
    14881 ctttaccttt atggtaaatg gtatggttag ttttctttcc tgcagcatta atcacatagg
    14941 attcagactg gttatggcgt tcagtccatg catcggaata gatgtattcc gcgccgaagt
    15001 agggttgatg cgaaacatca cccacggcga atttttgcca gtccaggcgt gttttgaagg
    15061 tgtaattatc tacagcctgg gaaatgtgtc ctaatccgcc acgggtacaa cgccctgtaa
    15121 tatcaccata tgtacatgaa agttcggtgt accagatgtc atgatcgtga cgggtataat
    15181 cactaatatg atcccaacca acggtggtac gtagtttggc ccatgcgagt tgcgtatcca
    15241 tatcccatgc cagaccatag gatttattac ccatttcgcg atcagactgc gggaaggtgc
    15301 tggtattata atcacggctg gagccggtat attttaaggt taaatcgtgg gtaaagcggt
    15361 cgctggcaaa ccaggtaaat ttgctcaatg cagtatcgat aacgttttta tactgtgccc
    15421 gaccggcgac aatgccgtca ttcgaaacat aatccgcgcg ggtgatatca gactggcggc
    15481 gcgataaacc ggcggtaacg ccaaagttat cagccagctc ctgattaaac gacaaggtat
    15541 aaaagttctt tttaaaatct ggagagaaat aggtacttcc tgaagaacct tgattaaatg
    15601 cgctcttgtt attctcatcg atatgcgatg ttaaccagtc caaacgcgta gtgcgataac
    15661 ccaatttcac cttgctatca tcagcgttga agcgtttgat ctttgcatca attaccccgc
    15721 cattgaagcg accaaattca accggcacaa aactgtcata aagcgtcaca ttgtccagta
    15781 agctgacatc aagataatac ccctgtgaca tcccgctaat attggttgca ctactggcat
    15841 cggactcatt cgctgggttc aggttattag ttgcactaat accgtcaatc aaataggcat
    15901 tctggtaggg cgacgcaccg tgaatagaga ttttctcagg gcgaatatct ccctggttca
    15961 acgaggtact ttgcgttgaa tccatgcgta ccgcagggtt ggttctcagc agatcgctga
    16021 tattgccatt cccggtcggt aaacgctcga tgctttcact ggtgtaatgc gtgttgccgt
    16081 ttacgggtac agggacagga gcatagaccg tcatctcttc aaaaaaatac atgtacatgt
    16141 ttttgtcatc gacggcctgc gccacatcag cgccacataa aatgacgcca ggaataagaa
    16201 ctcgcttcat ttatttatat ctccgtacag cagggctttt attgttgtta taaaaccgtg
    16261 ctaatgtcac aaatatcatc agccaggttc cagacaccgg gttgatgtgt aaccataatg
    16321 acgccgcttg tgggtagttt ttcacgcact aaacgcagta agcgtatagc ctcttgttcc
    16381 tcaagatgag aggtagtttc gtcaagaaat atccatttcg gacgtcgtaa aattaatcgt
    16441 gccagggcga tacgttgttt ttcgccgctg gaaagaatat ctccccagcg atcgtggtca
    16501 tgaatacgcg cagccaattt tcccagacca acctgatgca gtacttcgct caacgattta
    16561 tcgtctacgg gcaggggaag tgctttacaa ataatctctt tcagtaagcc gcttttgatt
    16621 aacggtgttt gtgacacata ccaactgtca gcaggagaag aaatatcacc tttaaaccac
    16681 ggccagcagt gggataatgt tttaagcagt gtggtttttc ccgcaccaga gtagcctttc
    16741 agtaatagcc atttgcctgg cgaaacatga aagttcaagt tctctaatat gatcttatta
    16801 tcaggcgtac gaatactcgc attagccact tgtaccgcat gttggcaatt tttaggctta
    16861 ttcgtagggc gctattcagt gagttgatgg aactcataca agcgatcgat aaccgcagcc
    16921 agttcagcaa gttcgtcata tttataaata aaccagctta aattgttcga tactagcata
    16981 aatgcctggc gcgatttcat cagtccgccc agattgatct gcccgctaat aaactgcggc
    17041 aataacagaa agtagggaag aacgctaagc gagcgcgaat aaatattttg ccagtaatca
    17101 agccaccgct gacgattcat taaacgatgc cagttctctt taatggtatg aaaattgtcg
    17161 cgcaactcct ggcgttgtag agactcagca ttgcttagcg caatcagttc tgcctgctta
    17221 ttatgctgca caagattagt tcgaaatgtc gcttcgctac gttgtttttc cacattaagc
    17281 ggacgaatac gtttacccac cttatgggta aataaagttc caccgatcac aatgagcacg
    17341 acggtataga ccatataccc ctggatattc cattctgttc caccaacagt gaatgagagc
    17401 gtacccgcgc tttgccaaag aataacggta aaggtgatca gcatgctaag tgactggatg
    17461 aagccaaatg aaagactgag cgttttgttg atcagtaaga gaatgtcttc agcgatacgt
    17521 tggtcagggt tatctgtatt tttatgctcg ccgtagatct gcgtgaagta gtagttttta
    17581 tctgcaaacc agcgattaag gtagtaatct gttaaccatt cgcgccagcg aatagtcagt
    17641 agtttaatta accatgtttt gtttacggat attaatacaa agatccccag cagggcaggg
    17701 aaccaaagaa cgagctgcca aagcttatcg gtttctttct ggcttagcgc attgaaaaaa
    17761 tcattattcc agtcgtttaa ccagacctga attttaacca cgccaaggat cattgcgagg
    17821 ataataatga ttaacaacac tgaagtttta ttattcttac gcagccaaaa gggcttcagc
    17881 agacagagat actttgctat caacatacaa aacgtaatgg gaatggttat cattagcgaa
    17941 aattgattgt gccatccaat gattatctat ggcaatgatt tactgatgaa atttatttaa
    18001 gcatcattta acaatatgaa atat
//
LOCUS       DAEBXD010000078        17820 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEBXD010000078 DAEBXD010000000
VERSION     DAEBXD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17820
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.078"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..186)
                     /locus_tag="KJL94_002847"
                     /pseudo
     CDS             complement(1..186)
                     /locus_tag="KJL94_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(287..1018)
                     /gene="rlmB"
                     /locus_tag="KJL94_002848"
     CDS             complement(287..1018)
                     /gene="rlmB"
                     /locus_tag="KJL94_002848"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBD4411128.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1198..3639)
                     /gene="rnr"
                     /locus_tag="KJL94_002849"
     CDS             complement(1198..3639)
                     /gene="rnr"
                     /locus_tag="KJL94_002849"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBD4411129.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3678..4103)
                     /gene="nsrR"
                     /locus_tag="KJL94_002850"
     CDS             complement(3678..4103)
                     /gene="nsrR"
                     /locus_tag="KJL94_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBD4411130.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4308..5606)
                     /gene="purA"
                     /locus_tag="KJL94_002851"
     CDS             complement(4308..5606)
                     /gene="purA"
                     /locus_tag="KJL94_002851"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBD4411131.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5710..5907)
                     /locus_tag="KJL94_002852"
     CDS             complement(5710..5907)
                     /locus_tag="KJL94_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBD4411132.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5989..6993)
                     /gene="hflC"
                     /locus_tag="KJL94_002853"
     CDS             complement(5989..6993)
                     /gene="hflC"
                     /locus_tag="KJL94_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBD4411133.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6996..8255)
                     /gene="hflK"
                     /locus_tag="KJL94_002854"
     CDS             complement(6996..8255)
                     /gene="hflK"
                     /locus_tag="KJL94_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBD4411134.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8341..9621)
                     /gene="hflX"
                     /locus_tag="KJL94_002855"
     CDS             complement(8341..9621)
                     /gene="hflX"
                     /locus_tag="KJL94_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBD4411135.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9697..10005)
                     /gene="hfq"
                     /locus_tag="KJL94_002856"
     CDS             complement(9697..10005)
                     /gene="hfq"
                     /locus_tag="KJL94_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBD4411136.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10091..11041)
                     /gene="miaA"
                     /locus_tag="KJL94_002857"
     CDS             complement(10091..11041)
                     /gene="miaA"
                     /locus_tag="KJL94_002857"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBD4411137.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11034..12881)
                     /gene="mutL"
                     /locus_tag="KJL94_002858"
     CDS             complement(11034..12881)
                     /gene="mutL"
                     /locus_tag="KJL94_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBD4411138.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12891..14228)
                     /gene="amiB"
                     /locus_tag="KJL94_002859"
     CDS             complement(12891..14228)
                     /gene="amiB"
                     /locus_tag="KJL94_002859"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBD4411139.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14247..14708)
                     /gene="tsaE"
                     /locus_tag="KJL94_002860"
     CDS             complement(14247..14708)
                     /gene="tsaE"
                     /locus_tag="KJL94_002860"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBD4411140.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14680..16227)
                     /gene="nnr"
                     /locus_tag="KJL94_002861"
     CDS             complement(14680..16227)
                     /gene="nnr"
                     /locus_tag="KJL94_002861"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBD4411141.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16226..17365
                     /gene="queG"
                     /locus_tag="KJL94_002862"
     CDS             16226..17365
                     /gene="queG"
                     /locus_tag="KJL94_002862"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBD4411142.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17348..17401)
                     /locus_tag="KJL94_002863"
     CDS             complement(17348..17401)
                     /locus_tag="KJL94_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411143.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17634..17709)
                     /locus_tag="KJL94_002864"
     tRNA            complement(17634..17709)
                     /locus_tag="KJL94_002864"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17674..17676),aa:Gly,seq:gcc)
     gene            complement(17745..17820)
                     /locus_tag="KJL94_002865"
     tRNA            complement(17745..17820)
                     /locus_tag="KJL94_002865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17785..17787),aa:Gly,seq:gcc)
BASE COUNT         4052 a         5072 c         4483 g         4213 t
ORIGIN      
        1 cgccattcat ggccatatca atgattttct ggtgcgtacc gggctgagag gcggtgtaag
       61 tgaactgtag ttgccatgtt ttacggcaat gagagcagag atagcgctga tgtccggcag
      121 tgcgccgtta cgcaccacgc cttcagtagc ggagcaggaa ggacatctga tggaaatgga
      181 agccacgcaa gcaccttaaa atcaccatca tacactaaat cagtaagttg gcagcattac
      241 caaaaaaacc atccaaatct ggatggcttt tcataattca gaggaactag ctgcgctgac
      301 gaaccgcttc aaataagcaa attccggttg caaccgaaac gttcagggaa gaaacacttc
      361 ctgccattgg gatgctgatc aactcatcgc aatgttcacg ggtcaggcga cgcatacctt
      421 caccttccgc gcccatcacc agcgccaggc gtccggtcat tttgctctga tagagtgtat
      481 gatccgcctc gcctgccgta ccgacgatcc agatattctc ttcctgcaac atacgcatgg
      541 tgcgcgcaag gttagtcacc cgaatcagcg gaacgctttc tgccgcgccg caggctactt
      601 ttttcgccgt agcgttgagc tgtgcggagc gatctttcgg cacaatcacc gcatgaacgc
      661 ctgcggcgtc cgcactacgc aggcacgcgc cgaggttgtg cggatcggtt acgccgtcga
      721 ggatcagcag gaacggttga tcgagcgaag cgatcagatc cggcagatcg ttttcctgat
      781 actgacgtcc tggcttcacg cgggcgataa tgccctgatg cacggcaccg tcgcttttct
      841 cgtcgagata ttggcggttt gccaactgga taaccacgcc ctgagactca agggcgtgaa
      901 tcagcggtaa cagacgttta tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt
      961 caggggcgcg ctccagcagg gcctgcactg cgtggatgcc gtaaatcatt tcgctcatta
     1021 atgtactcgt tgtttacgtt ttgcctgatg cactacgttt atccggccta catgatctct
     1081 gcaatatatt gaatttgcgt gattatgtag gccggataag gcgttcacgc cgcatccggc
     1141 atgaacaaag cgcattttgt cagcaatcta accctctttt tttaaagagg gtattgatca
     1201 ctctgccact tttttcttcg ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt
     1261 tttcgccgat ggctttttcg cttttctcgc gtctttcttc gccgctttcg gcttcgtttt
     1321 tttctcaccg cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg
     1381 cttgccgcct tttttgcctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac
     1441 gttgcgcggt gcgcgttcac tggagatcag actaaagtcg attttgcgct cgtccatatt
     1501 aaccgcttcg acgcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc
     1561 ccccatcagg cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac
     1621 atggaccaga ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac
     1681 gctggaaatt acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag
     1741 ccagtcagcg acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg
     1801 acccagttgc aacatctctt ccatcgaata atggtagccg ccggtttcag tggtgttgcc
     1861 ctgatgcccc tgctctttcg ccagcagata tttaatggcg cggtgcagcg tcaggtcagg
     1921 ataacgacga atcggcgaag taaagtgcgc ataggactgc aatgccagac cgaagtgacc
     1981 acggttttct ggatcgtaaa tcgcctgttt catcgagcgt agcagcatgg tttgcagcat
     2041 ttctgcatca ggacggtcgg caaccgactc cagcagttcc gcgtagtcac gcggttccgg
     2101 cttgttacca cccggcagct ccagccccag ctccgccagc actgaacgga aagaggtaat
     2161 cgcttcggtg ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac
     2221 gaaacgcgcc gccgagatat tcgccagaat catgcactct tcaattaatt tgtgcgcgtc
     2281 gttacgctgg gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc
     2341 ttcttcgctc tcaaatgaga tcccaccgcg ttcttcacgg gctttatcca gcactttata
     2401 gaggttatgc aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg
     2461 atcgccctgc agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac
     2521 cgcttcgtag aatttgtagc ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac
     2581 catacacagg cggtctacct gcgggttgag cgaacacagg ccgttagaga gcacttccgg
     2641 cagcatcggg ataacctgcg aagggaagta caccgacgtg ccacggttac gcgcttctct
     2701 gtccagcggc gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg
     2761 ccagccgccg ccgcgttttt tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc
     2821 gccatcaatg gtgaccagcg gtaaatcgcg caaatcgaca cggcccgctt ttgcttcttc
     2881 cggcacttct tctttcagcc ccgcaacctg ttgctcaaca gcctgcggcc agatatacgg
     2941 aatttcatgg gtacgcagag cgatatcaac cgccatgccg gtgcccatat tgtcgcccag
     3001 cacttcgacg attttaccca ccgctttggt gcggcgagtc ggacgctgag tcagttcgac
     3061 tacgaccaca aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa
     3121 gctcagacgg ctatcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc
     3181 aacaatctgg ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt
     3241 acggtcagca cccagcggct gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg
     3301 ctcgctggag agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc
     3361 acggtgacca ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg
     3421 acgacgagtg aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc
     3481 ttcaagctgc tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg
     3541 tttttcacgt ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta
     3601 tttttcagct tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc
     3661 tctgatgaag attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca
     3721 accaaatcgg caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa
     3781 agcgcctgtt tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc
     3841 agcgataagg gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccggttta
     3901 cccaggcgaa tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt
     3961 tgattgatta ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa
     4021 atactggtca tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa
     4081 tcagtgaaac tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt
     4141 atattgatga taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa
     4201 aaaagcgagg atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta
     4261 tcacactgtt agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac
     4321 gggtcgcgca gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc
     4381 acaccggtca gctcttcaat acgcttgatg tagttcagcg ccgcctgcgg caggccgcta
     4441 cgatctttca cgccgaaagt ggattcagac cagcccggca tggtttcgta aatcggctct
     4501 acacctttcc agtcgtcagc tgccagcgga gtggtagtca cttcgcgacc atccggcata
     4561 cggtaagcca cgcagagttt aacctctttc aggccatcca gaacgtccag tttagtcagg
     4621 cagaagccag acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag
     4681 ccggtacgac gacgacgacc cgtagttgca ccgaattcgt taccctgctt gcagaggaac
     4741 tcgccagttt catcaaacag ttcggccggg aaaggacctg cacctacacg agttgagtaa
     4801 gctttgagga tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc
     4861 acgccaccag cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc
     4921 agcagcgtac cctgcgcacc ttcgaacatg acgaaatcgc cacgctgacg cgcctggtcg
     4981 agcaggtcag aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca
     5041 tccagaactt tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg
     5101 tgatattcca tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca
     5161 acacgcagac cgcgacgtgc cactttatct tcataagcag gcccgatacc acgaccggtg
     5221 gtgccgatcg ctttcgcgcc acgcgctttc tcacgcgcgt tatccagagc aacgtgataa
     5281 tcaaggatca gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg
     5341 tcttccagtt ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg
     5401 atgatgctgg ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt
     5461 tcaccgttga ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat
     5521 ttagcccgtt cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg
     5581 cccagtacga cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc
     5641 taccatcgct ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct
     5701 gttttttgtt cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac
     5761 aagtccaccg ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat
     5821 cttcttccat gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc
     5881 aagcgccagc cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac
     5941 atccctgagg atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc
     6001 ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc
     6061 ctgattgcca gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc
     6121 cggatcttta ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg
     6181 cataatgcgg ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt
     6241 cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc
     6301 acgctcggcg cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg
     6361 cttgatacgc acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac
     6421 cggaactttg cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc
     6481 tgctgccggg gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg
     6541 tacttcgagg gtcagacgac cacgggaatc ggtgacgata tctttcacgt ccaggcgacc
     6601 aatttcagaa cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat
     6661 gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta
     6721 agagtcgacg atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat
     6781 ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag
     6841 accaggctca taaaccaaag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag
     6901 cgtaataccg cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac
     6961 gatgatgata atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg
     7021 ttacgctgcg cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg
     7081 ttgcttgctc cactggttgt ggaggaagag gctggcggca gacgcagcag attgctggca
     7141 ccgttatcgc tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga
     7201 accatcaggt tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt
     7261 tccatcgtct cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc
     7321 agaagtttag caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac
     7381 gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg
     7441 gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg
     7501 tcaaacgccg cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcagc
     7561 gtgataccca tgtcatacgg acgaatcgtc tcttccagtt cgcgctgagt atcgctacga
     7621 atcacggtac gaccttccgt cagaatgcgg tccatggtgt atttaccgat aactccacgc
     7681 agggcgctgt cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat
     7741 ttttccggat tggtgacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc
     7801 gacgtcagca tcacaccaga agcggccagt tcacgcacgg cttccacgtt caccggtttg
     7861 acttcgtcga taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta
     7921 ccaaagcgtg ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc
     7981 cagataatga caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc
     8041 gggccttgcg atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg
     8101 agctttttgc tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg
     8161 cctttgtttc catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg
     8221 tcttgtccgt tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg
     8281 gtgatccctg ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg
     8341 ttagatcagg taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat
     8401 cggcatacga acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc
     8461 ctgaagctga taaaaacgac tcctcagacg cccttcctgc ggtggcagac gcaatgtatg
     8521 ctgcgccacc tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc
     8581 tccggtctgt gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg
     8641 cggttcgaaa tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg
     8701 agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc
     8761 cgccgcatca atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt
     8821 aaatgccgcc accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac
     8881 ggtttcaccg acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa
     8941 ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc
     9001 gttggtatat cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc
     9061 ctgctcacgc tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg
     9121 caacaaacga cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc
     9181 tttctgtctt tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg
     9241 cgccagctca acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa
     9301 aataaggccg gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg
     9361 cgccgggctc agggcatggt caaaaagaac gaccgaagca cccatcgctt tgacagcttc
     9421 cgcaatttca actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct
     9481 accggtaatc acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg
     9541 gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc
     9601 agcatcataa cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga
     9661 tcgctggctc cccgtgtaaa aaaacagccc gaaaccttat tcggtttctt cgctgtcctg
     9721 ttgcgcggaa gtattctgcg cgctgctacc atgatggtag ttactgctgg taccgccacc
     9781 ggcgttgtta ctgtgatgag aaaccgggcg agatgggaca acagtagaaa tcgcgtgctt
     9841 gtaaaccatc tggctgaccg tgtttttcaa caggatcacg aactgatcaa aagactcgat
     9901 ttgcccttgc agcttaatac cattcaccaa ataaatagaa actggaacac gttcccgacg
     9961 cagtgcgttc aggaacggat cttgtaaaga ttgcccctta gccattctct cttttcctta
    10021 tatgcttatt tgtactttga acctttcgat tctgaaaaaa ttgcgcacga tacgtctcaa
    10081 ttgtacacat tcagcctgcg atagcaccaa caacctgtaa tacttcgtca cgcgcctgtt
    10141 ctggtttttc actgtcaagc cagtgaaccc cttcccaacc acgcagccag gttatctgcc
    10201 gcttcgccaa ctgtctcgtg gcgcaaacac ctcgataaac catttcatcg tatgagattt
    10261 cgccttcaag gtaagaccac atctggcgat aacccacgca acgaatggaa ggcaagtccg
    10321 tatgcaaatc tcctcgggca aaaagcgccc ggacttctgc ttcaaaacct gaagccaaca
    10381 tctgatgaaa acgctgctca atgcgttgat ggagcagttc acggctcgcc ggggcgatgg
    10441 cgaactgatg cacctgatac ggtagagcgt ctcctgacgt ttgcgtcagt tccgttaaag
    10501 ttttacccga aatgaaaaaa acttccagtg cccgggaaag cctttgtgga tcatttggat
    10561 gaatccttgc tgccgcaacc ggatctacct cctgaagttg acgatgcaat gactcccaac
    10621 cttgctctgc cgcctgttgc tcaattctgg cccgcacttc cgggtctgcc gacggtagcg
    10681 gcgacaaccc ttccagcaat gccttgaaat acaacatcgt accgcccact aacagtggga
    10741 tccgccccgc cgcggtgata tcggccattt ccgccagcgc atcgcggcga aaatcagcag
    10801 ccgagtaagc ctgcgacgga tcgcgaatat ccagcaatcg gtgcggcgcg gcgagtaact
    10861 cttcagcgtt cggcttcgcc gtcccgataa ccatcccttt gtaaataagg gcagaatcaa
    10921 cgcttatcaa ctctactggt aaaattttac gcagctcaat ggctaacgcc gttttaccgg
    10981 aggccgtcgg ccccatcaaa aaaatcgcct taggcaggct cgccttactg atatcactca
    11041 tctttcaggg cttttatcgc cggatgtaaa tcaacagatt gtaacagacc acccggcggc
    11101 gttttcacaa gttgcggaca taaccgttcc acgtccgcca gcagggttat ggcctgtgcc
    11161 attgaccact gcgcatgttc gctcatcaga tttcgtgcaa tccactgcgc aatattgcca
    11221 ggttcgaata cggactgctt cgccaggtag cctatcagtt caggaatcaa gatttgtaaa
    11281 ttttgttggc gtaagggtaa aggcacggcc ctgatggtca catgctgtgc atctgactgg
    11341 aaatcaatac ccaattccgc tagggcagac tgcgcttttt ctaatgccga tttttcttcg
    11401 ccagaaactt ttagccgcaa cggaatcagc agcggctggg cgcaaacggg cgcttcaccc
    11461 ggcgtcaatt gtgcctgacg cagccaacgt tctgccactg gcaaggataa aagtgaaatg
    11521 ttgccatcgc gctccagcaa cgcacagtcg gaatggacga tagtcagtac ccgaccaaaa
    11581 ctctgactgt tcgccgcaag tgcaggttcc tgcggttccg gcgcttttgg tttttgcatc
    11641 ggcgcgggcg tttgcaaaag ctggcgatac acttcacctt gctgtttctg gtagcctggc
    11701 tgcgcattcg gccagggggc agccggacga ctgcctgagg cgggcgcagg agagtagcgc
    11761 ggagctaccg gctcacgagc tgccggttct gcaaagtgat tgcgccccgc cgccacgcgg
    11821 ttttccggga tggcacgcgg tgcgggttgt ggttcatcgt ccagcggtag cggcgtttcc
    11881 agttgctgtt gtagcacgct cagcacgccc tgatagataa aatcatgcac cagacgcgac
    11941 tgatggaaac gcacttcgtg tttggcgggg tgtacgttga cgtccacctg atgcgggtcg
    12001 atctccagat acaacacaaa tgccggttgc tgatcggccc ccagtttgtc ttcgcaggcc
    12061 tggcggatcg catgattgat caggcgatcg cgcatcatgc gaccattcac gtagcaatac
    12121 tgaatttccg ccagtgcggg cgtagtgtga tttggatcgg ccacccagcc gcgcagggtg
    12181 agatcgccgt gttgccattc aatcgccagc gcttgttcga gaaaagcggt gccgcaaatc
    12241 gcgcctaagc gccgttcttt ttgcccgcct tccggcactg cgcggtactg acgcacaatt
    12301 ttaccgttat gcgacaggtt gatcgtgacg tcgaaacgcg ccagcgcaat gcggcggatg
    12361 atttcatcaa tatggctaaa ttcggttttc tcggtgcgca ggaatttgcg ccgcgccggg
    12421 gtgttgtaga acagatccag cacctccagc gtcgttccca ccggatgcgc tgccggttta
    12481 accgtcacgt ccatgtcgcg tccttcggca taggcctgcc aggcttcctg ctgttctgcg
    12541 gtgcgtgaag tgagcgtcag gcgggaaacc gaactgatac tcgccagcgc ctcaccgcga
    12601 aagcccaggc tgataatggc ttcgagatcg tccagagagg cgattttact ggtggcatga
    12661 cgagccagcg ccagcgccag ctcatctttt ttgataccgc agccgttatc acgaatgcgg
    12721 ataagtttcg ccccaccgcg ttcgatatca atatcgatac gcgtcgcacc tgcatcgagg
    12781 ctgttttcca ctagttcttt gactaccgac gcaggtcgct cgaccacctc acctgcggca
    12841 atctggttcg ccagttgtgg cggtaagacc tgaattggca tcaatcgtcc ttagtttggc
    12901 agcgtgcgat ctggcgtcgt caccgtactg gcagtttgtg ccgttgcacc ctgcggcgca
    12961 gattgcatcg gatgcgcaag gaagtaattg cgcaggcctt tgtaaatggc ttctgccagc
    13021 tgttgttggt aatcgtcgct cgccagcaaa cgttcttcgc tgttgttgct gataaaaccg
    13081 gtttcgacca gtactgatgg gatatccggc gagcgcagaa cgccaaggct ggcgtgttct
    13141 ggtcgacgtt tatgtatttc gccaatgcgt tgcaactgac tgatcatact ggtcgctaca
    13201 tcatacccta cccgctggga atgaccgaac tgtaaatcca gcaccgcctg gcttaaatag
    13261 gggtcagact gactgttcgc cagcacatca cccgccccac ccagcagctc cgacagtttc
    13321 tcgtactgct ccagccagct ggccatttca ctgttggcgc gacggttaga gagcacccat
    13381 acggaagcgc cagtcgcact acggttcggt gcggcatcag cgtgaatcga cacgaggaaa
    13441 ttggcgtttt gcttatgtgc cacatcactg cgccccatca ccgagataaa gtaatccccg
    13501 tcacgggtta aaacgccttt aaacatcgga tcgtcattga gcaaagtacg caatttacgc
    13561 gcgatggcga tggtgacatt tttctcccgc gtaccaccgg ggccgatagc gccagggtcc
    13621 tgaccgccgt gtccggcatc aatagcgatg ataattttat cgccagtgtt agccgtcgcg
    13681 cgcgctgccg gacgcgttac cgtattactg ctgataacac ccgtagtgcg gttactttcc
    13741 gttttaaacg gattgcgcgc cggttcgctg acgcgcggtg cgacaaccgc aggcgtttca
    13801 acgcgtttcg caaccacggg cggcggagga ggcggtggcg gcacatcggc gttaatcgta
    13861 aagacgacag tgtaattgct gccattctgc cgcttcaccg cttcggtttt accgttttcg
    13921 gtaagatcga ccaccagccg tagcgtttgt gcatctttag gcgttccaga gcgaatcgcc
    13981 ttcaccagat tattgccgct gaacaacaac ggcagtccct gaatcacgcc cgtttgtttg
    14041 atatcgagcg ccacggtgcg tttgctttga tggctaaacg cataatcagg atcgccaata
    14101 aaactcaacg ttatccgcgc ctgttgatta ccgttagaaa cctgaatatc agagagcgtc
    14161 gcggcaccca ccggcgtgca cagcagcagc agcgtcgcta ccaaccaatt tctgatgcga
    14221 tacatcatcc cgccaccttt caaaggttaa ccggctaaac gcgccagcaa caattcaccc
    14281 gcagaggaaa ccgcactcac gcgcgcctca cggccttgtg cctgataatc aatgtgtatt
    14341 tcgacatccg ggtcaggaag aacacctgta ccttgttgtg gccactccac caggcagatg
    14401 gcatcgttgg caaaataatc gcggatcccc ataaactcca gctcctcggg atcggcaagg
    14461 cggtacaaat caaagtgata gaccattaag ttgtcgagcg tatagggttc gaccagcgta
    14521 taagtggggc ttttgacatt accctgatga cccagagcct gtaaaaagcc ccggctaaag
    14581 gtggttttac ctgcgcctaa atcgccatac agatagatta cggttgcgcc atcgcaggct
    14641 ttcgctaccc gctcgcccag gtctaatgtt gcctgctcat cagggagcgg aattactcga
    14701 ttcatcatgg tttttatcag tcacttccgg gttaacaata cgctgtagcg tggaaaagag
    14761 atcggttgcc agcatcccgc gcgttccaaa acgcgccgcc agtacgtcag ctgccgcacc
    14821 gtgcgcgaca cagcctgcac aggctgcatc atacggcgac agtttttgcc caagcaatgc
    14881 gccaataata ccagagagca catcgcccat gccgccgctc gccatgcctg catttccggc
    14941 atcaataatg cctaaagcgt caggatgggc ggcgaccacg gttccggcac ctttcagcac
    15001 cgctacgccg ccataacgtt gtaccagacg tttggcgcaa tgtaagcggt cactttcaat
    15061 ttcagcgacg gaacagccta acaaccgtgc ggcctcgcta ggatgcggcg tgatcacgcg
    15121 attgtgacgc ttatcgggat tgattgccag caggttcaat gcatcggcat cccacaacat
    15181 cggtttgcga aaattctcaa ctttttgcag ggcttttttc ccccactctt gctggcccag
    15241 accgggacca atcaccacca catcggccca ttccaggctt tcggtaagag agtccatagt
    15301 cagttcatgc accatcaatt ctggtcgtgc agtcagcagc ggcgcaatgt tctcactgcg
    15361 ggtcagtact cggactaaac cagcaccagc acgtagcgcc gcttcccccg tcatacgaat
    15421 agcccccgcc gtgccgtgat cgccaccgat aatcaccagc cgcccgtgat cgcctttatg
    15481 cgaagtcggg cgacgcggtt tcagccagtg agaaagttgt tctgccgaaa accgctgaat
    15541 tttcgtctcc tgacctgcca gccagctatc cagccccagt gagtcaaaat gcagttgtcc
    15601 tgtaacatcc cgcgcttttc cagtgagcaa gcctagtttc agcgcaataa aagtgatggt
    15661 gtgatcggcg ttgatcactg cgcctggcgt agcgccggtt tcagccagca ggccggaagg
    15721 gatatcaacc gccacaatcg gcgcaggatg ggaattagcg tggtcgatta actggctaat
    15781 ggattcgcgg ggcgcttgct gcaagccggt accgagcagc gcatcaacaa tcagatctac
    15841 cgattcgggc cagacaatat tcgaagcatg gatctcgcca cccgcgttta accatgcttc
    15901 gcgtgccagc gcggcctctt ccggcaacgg tttgtcgctc tcctgggcca acaacgtgac
    15961 ctcaatgccg accgctttgg ccagtcgcgc gaccacgtag ccatcgccgc cgttattacc
    16021 atgaccgcac agcaccagcc agtggcgggc gtcaggatac gccgaacgac acacctggaa
    16081 tgcggcctcg ccagcgcgaa gcatcagctc atagagtgtg agccccagca catctgccgc
    16141 ctcgcgttct ccgcggcgga tatcgtcggc gtaccagacg gtgtgtggta tacttacggg
    16201 gtttttcttc attgtatggt ccgtcatgtc agagcccctc gatctcaatc agttagcgca
    16261 aaaaattaaa cagtgggggc tggaactggg ctttcagcag gtaggtatta ccgataccga
    16321 tctcagcgag tccgagccca aactgcaagc atggctggac aaacaatacc acggcgaaat
    16381 ggactggatg gcacgtcacg gtatgctgcg cgctcgcccc catgagttat tacccggtac
    16441 gctgcgtgta atcagcgtgc ggatgaatta ccttcctgct aacgccgcat ttgccagcac
    16501 gctgaaaaac cccaaactcg gctatgttag ccgttatgcg ctgggccgtg actatcacaa
    16561 acttctgcgc aaccgactca aaaagctggg cgagatgatt cagcaacatt gtgtttcgct
    16621 gaattttaga ccgtttgtcg attctgcgcc tattctcgag cgcccgttag ctgaaaaagc
    16681 tgggctcggc tggacaggta agcactcact tatcctcaat cgcgaggccg gttcgttctt
    16741 ctttttaggc gaattgctgg tcgatattcc gctgcccgtg gatcaaccag tcgaggaagg
    16801 atgcggcaaa tgcgtggcct gtatgacgat ttgcccgacc ggtgccatcg tcgagccata
    16861 taccgtcgat gctcgccgct gtatctctta tctcaccatc gaacttgaag gggcgctccc
    16921 ggaagagttg cgaccgttaa tgggaaaccg tatttacggt tgcgatgact gccagcttat
    16981 ctgcccgtgg aatcgctatt cgcaactcac tacagaagac gatttcagcc cgcgtaagcc
    17041 gctacacgca ccggaactca ttgagttatt tgcctggagc gaagagaagt ttttaaaagt
    17101 cacggaagga tcggcgattc gccgtatagg tcacctgcgc tggctgcgta atatcgccgt
    17161 ggcattaggc aatgcccctt gggatgaaac gattttgaca gcgctggaaa gtcgtaaagg
    17221 tgagcaccca cttctcgatg agcacatagc gtgggcgatt gcgcagcaaa tagagagacg
    17281 aaatgcgtgc atagtcgaag tgcaattgcc gaaaaaacag cgtctggttc gggtgattga
    17341 aaaagggtta ccgcgtgacg cctgattcat ccacagcttg tgaataaaaa taaaaacgca
    17401 ttgcaattca agacgaataa aatcgtcaag tgatcgactc gacaaaacgg aatcttgatt
    17461 tttaacttat ttatcaatag gttaaaaaat accattcacc gtgcgaagtt tctttgaaat
    17521 taagattaca gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacaa
    17581 aaaccagcaa taacgcatcg cgtcgctgta gatattttat tgagagaaga atttggagcg
    17641 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
    17701 tattcccgct tgggtggtct gtgccttaca gcacttttaa attttggagc gggaaacgag
    17761 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
//
LOCUS       DAEBXD010000079        17548 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEBXD010000079 DAEBXD010000000
VERSION     DAEBXD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.079"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="KJL94_002866"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="KJL94_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="KJL94_002867"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="KJL94_002867"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBD4411144.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KJL94_002868"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KJL94_002868"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBD4411145.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="KJL94_002869"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="KJL94_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBD4411146.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="KJL94_002870"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="KJL94_002870"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBD4411147.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="KJL94_002871"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="KJL94_002871"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBD4411148.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="KJL94_002872"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="KJL94_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBD4411149.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="KJL94_002873"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="KJL94_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBD4411150.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="KJL94_002874"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="KJL94_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBD4411151.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="KJL94_002875"
     CDS             11402..11617
                     /locus_tag="KJL94_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBD4411152.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KJL94_002876"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KJL94_002876"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBD4411153.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="KJL94_002877"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="KJL94_002877"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBD4411154.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="KJL94_002878"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="KJL94_002878"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBD4411155.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="KJL94_002879"
     CDS             complement(16353..16892)
                     /locus_tag="KJL94_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBD4411156.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="KJL94_002880"
     CDS             complement(16908..17426)
                     /locus_tag="KJL94_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD4411157.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4080 a         4352 c         4866 g         4250 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttat
//
LOCUS       DAEBXD010000080        17364 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEBXD010000080 DAEBXD010000000
VERSION     DAEBXD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17364
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.080"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(271..525)
                     /locus_tag="KJL94_002881"
     CDS             complement(271..525)
                     /locus_tag="KJL94_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBD4411158.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            835..2607
                     /gene="aspS"
                     /locus_tag="KJL94_002882"
     CDS             835..2607
                     /gene="aspS"
                     /locus_tag="KJL94_002882"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBD4411159.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2725..3177
                     /gene="nudB"
                     /locus_tag="KJL94_002883"
     CDS             2725..3177
                     /gene="nudB"
                     /locus_tag="KJL94_002883"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBD4411160.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3206..3946
                     /locus_tag="KJL94_002884"
     CDS             3206..3946
                     /locus_tag="KJL94_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4411161.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3981..4502
                     /gene="ruvC"
                     /locus_tag="KJL94_002885"
     CDS             3981..4502
                     /gene="ruvC"
                     /locus_tag="KJL94_002885"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBD4411162.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4504..5106)
                     /locus_tag="KJL94_002886"
     CDS             complement(4504..5106)
                     /locus_tag="KJL94_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBD4411163.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            5175..5240
                     /gene="yobI"
                     /locus_tag="KJL94_002887"
     CDS             5175..5240
                     /gene="yobI"
                     /locus_tag="KJL94_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBD4411164.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5379..5990
                     /gene="ruvA"
                     /locus_tag="KJL94_002888"
     CDS             5379..5990
                     /gene="ruvA"
                     /locus_tag="KJL94_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBD4411165.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5999..7009
                     /gene="ruvB"
                     /locus_tag="KJL94_002889"
     CDS             5999..7009
                     /gene="ruvB"
                     /locus_tag="KJL94_002889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBD4411166.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7156..7941)
                     /gene="znuB"
                     /locus_tag="KJL94_002890"
     CDS             complement(7156..7941)
                     /gene="znuB"
                     /locus_tag="KJL94_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBD4411167.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7938..8693)
                     /gene="znuC"
                     /locus_tag="KJL94_002891"
     CDS             complement(7938..8693)
                     /gene="znuC"
                     /locus_tag="KJL94_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBD4411168.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8772..9704
                     /gene="znuA"
                     /locus_tag="KJL94_002892"
     CDS             8772..9704
                     /gene="znuA"
                     /locus_tag="KJL94_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBD4411169.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9720..11042
                     /gene="mepM"
                     /locus_tag="KJL94_002893"
     CDS             9720..11042
                     /gene="mepM"
                     /locus_tag="KJL94_002893"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBD4411170.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            11162..12133
                     /gene="lpxM"
                     /locus_tag="KJL94_002894"
                     /gene_synonym="msbB"
     CDS             11162..12133
                     /gene="lpxM"
                     /locus_tag="KJL94_002894"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD4411171.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12264..13706)
                     /gene="pyk"
                     /locus_tag="KJL94_002895"
     CDS             complement(12264..13706)
                     /gene="pyk"
                     /locus_tag="KJL94_002895"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBD4411172.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKDY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13834..14703)
                     /gene="hexR"
                     /locus_tag="KJL94_002896"
                     /gene_synonym="yebK"
     CDS             complement(13834..14703)
                     /gene="hexR"
                     /locus_tag="KJL94_002896"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBD4411173.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15040..16515
                     /gene="zwf"
                     /locus_tag="KJL94_002897"
     CDS             15040..16515
                     /gene="zwf"
                     /locus_tag="KJL94_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBD4411174.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16750..>17364
                     /locus_tag="KJL94_002898"
     CDS             16750..>17364
                     /locus_tag="KJL94_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD4411175.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPVMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRM"
BASE COUNT         4429 a         4293 c         4517 g         4125 t
ORIGIN      
        1 caacagcgtg atgcgtatct ttaccgacat caacgccgat aaagacctgg acggattcgt
       61 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacgggg
      121 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      181 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      241 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      301 agtaacgggg gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac
      361 tcgcaccaga aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg
      421 attaactacc tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa
      481 atcgatcacc accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa
      541 aacaaaatcg tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt
      601 aatttttatg agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc
      661 ccgatataaa gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa
      721 tttccagtat aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact
      781 tggtcacctg cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt
      841 acagaatatt gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt
      901 tgggtcaacc gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa
      961 ggtatcgtgc aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa
     1021 ctgcgtaatg agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat
     1081 attaaccgcg atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc
     1141 aaccgcgcag atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg
     1201 aaataccgct acctcgacct gcgtcgcccg gaaatggcgc agcgcctgaa aacccgcgct
     1261 aaaatcacca gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact
     1321 ccgatgctga ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg
     1381 cacaaaggta aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg
     1441 atgtccggtt ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc
     1501 gctgatcgtc agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg
     1561 caagtgcgtg aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg
     1621 gatctgggcg atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat
     1681 aaaccggatc tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt
     1741 gagtttgcag tatttgcggg gccggcgaac gatccgaaag gccgtgttgc cgctctacgt
     1801 gttccgggtg gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa
     1861 atctacggcg cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa
     1921 ggtatcaaca gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat
     1981 cgtactgccg cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt
     2041 gccgacggga tgggcgcact gctcctgaaa gtgggtaaag accttggtct gaccgacgaa
     2101 agcaaatggg caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc
     2161 ggcctgacgg caatgcacca tccgttcacc tcaccgaaag atatgactgc tgcagaactg
     2221 aaagctgcac cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa
     2281 gtgggcggtg gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt
     2341 ctgggtatca acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa
     2401 tacggtactc cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg
     2461 actggcaccg acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt
     2521 ctgatgactg aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag
     2581 gttgtgaaga aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc
     2641 atcaaggcgg caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc
     2701 gaaggcagtc aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc
     2761 ttagtggtca tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat
     2821 cccgatttct ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct
     2881 gccatgcgcg aagtaaagga agaggttacc attgatgttg tcgctgaaca actgacctta
     2941 attgactgtc agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg
     3001 ccgggcgtga cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag
     3061 atcgttttca ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc
     3121 actaagtcct ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca
     3181 ggcagggcta tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca
     3241 tcgtaaagct gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct
     3301 ggtaaccgcg gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc
     3361 ggtggataaa gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg
     3421 tggtgtgggc ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc
     3481 tggcggcacg gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga
     3541 agtgcgtcac gcattcagca aatgtggcgg taacctcggc actgacggtt ccgtagctta
     3601 tctgttcagc aaaaaaggcg tgatctcctt cgagaaaggc gacgaagaca ccatcatgga
     3661 agcagctctg gaagcaggtg ctgaagacgt tgtgacatat gatgacggcg cgattgatgt
     3721 ctacactgca tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa
     3781 agcagacagc gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac
     3841 cgcgccgaaa ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt
     3901 ttaccataac ggtgaaatct ctgatgaggt tgcagcgact ctctgatgat gcctgctaaa
     3961 cagcaaaacg gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga
     4021 ccggctacgg cgtcatccgc caggtaggta ggcaactgtc ctacctgggt agcggatgca
     4081 tacgcaccaa agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg
     4141 aaatcatcac ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga
     4201 acgctgactc agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtt gcggcggtga
     4261 atcaggaatt gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta
     4321 tgggtagtgc cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg
     4381 ctaatccaca ggcggatgcc gccgatgcgc tggcgattgc catcacccac tgccacgtta
     4441 gtcagaatgc gatgcagatg agcgaatcgc ggctgaacct gacgagaggg cgactgcgtt
     4501 aagttatacc gcctcggtca gttccggctg aggcgtttca actccctccg cgtgaatcaa
     4561 caccaaaccc ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat
     4621 agaacctaag aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag
     4681 ttgtccaaac gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa
     4741 gacaaatttt gaacagaact ggcgcgaaga ttcgtattta aaacccgtgt ggtaaagttt
     4801 gcgtagccgg gagggaacct gttcaacaat tcgttgtttt tgttgttctg tcagcccagc
     4861 gtctaatcgc tttatagcat agcgttgatt agacgaacgt ttaataaaac gagatagcgt
     4921 ggtgatggtt gaaaggggaa cccggctttc tgcaaccagg aagtcttcac cgttatgacc
     4981 gataatgatc ccgacgtgat tactccagca atttgatgca gctgatattt gaccaaataa
     5041 ggcagcacct atacatgtaa agacgatatc tccaatttca tattcagcag ggtaattaat
     5101 attcacgttg tcattccatt gaaatagata cgaatatacg taatagcata aaaaattatt
     5161 tttcaggagt cgcaatgtat atttttatta cgcatttctt cactgaatat gtaatattca
     5221 aatatttgct tccaatataa cctgttgaat aaattatact gtgccatttt tcagatcatc
     5281 gggacacctc gcaagttttc ttcatccttc gctggatatc tatccagcat ttttttatca
     5341 tacagcatta tctttgattc attacgcagg agcgtcatgt gataggcaga ctcagaggca
     5401 tcatcattga aaaacaaccc ccgctggtgt taattgaagt gggcggcgta ggctatgaag
     5461 tgcatatgcc gatgacctgt ttttatgaac tccctgaagc gggtcaggaa gcgatcgttt
     5521 tcacccactt tgtggtgcgt gaagacgcgc aactgctgta cggttttaac aataaacaag
     5581 aacgcacgtt gttcaaagag ttgatcaaaa ccaacggcgt cggcccgaag ttggccctgg
     5641 cgatcctctc cggaatgtca gcgcagcagt tcgttaatgc cgttgagcgt gaagaagtgg
     5701 gggcactggt gaaactgccg ggtattggca aaaaaaccgc cgaacgcttg attgttgaaa
     5761 tgaaagaccg atttaaaggt ttgcatggcg atctctttac gccagccgcc gacctggtac
     5821 tcacgtctcc tgctagcccg gcgaccgacg atgctgaaca agaagcagtt gccgcgctgg
     5881 tggcgctagg ctataaacca caagaagcaa gccgcatggt gagcaaaatc gctcgccctg
     5941 acaccagcag tgaaacatta attcgcgaag ccctacgcgc cgcgttatga ggtaaaggat
     6001 gattgaagca gaccgtctga tttctgccgg taccactttg ccggaagatg tagcagatcg
     6061 cgccattcgc cctaaattac tggaagagta tgttggtcag ccgcaggttc gttcacagat
     6121 ggagattttc atcaaagcag cgaaactgcg cggcgatgcc ctcgatcatt tgttgatttt
     6181 tggtcctccg gggttgggta aaactacgct tgccaacatt gtcgccaatg aaatgggcgt
     6241 taatttacgc acgacttctg gtccggtgct ggaaaaggcg ggcgatttgg ctgcgatgct
     6301 cactaacctt gaaccgcatg acgtgctgtt tattgatgag atccaccgtt tatcgccagt
     6361 tgttgaagaa gtgctgtacc cggcaatgga agactaccaa ctggatatca tgattggtga
     6421 aggtccggcg gcacgctcca ttaaaattga tttgccgccg tttaccctga ttggcgcaac
     6481 cacgcgcgca ggttcgctga catcaccgtt gcgcgaccgt tttggtattg tgcaacgtct
     6541 ggagttttat caggtgccgg atctgcaata tatcgtcagt cgcagtgcac gctttatggg
     6601 gcttgagatg agtgatgacg gtgcgcagga agtagctcgt cgcgctcgcg gtacgccacg
     6661 tattgccaac cgtctgctgc gtcgagtgcg tgatttcgcc gaagtgaagc acgatggcac
     6721 catctcggca gatatcgctg ctcaggcgct ggatatgttg aatgtcgatg ctgaaggttt
     6781 cgattatatg gaccgcaaat tgttgctggc ggtaatcgat aagttctttg gtgggccggt
     6841 aggtctggat aacctggcgg cagccattgg cgaagaacgt gaaaccattg aagatgtact
     6901 ggaaccttat ttgattcagc aaggcttttt gcagcgtaca ccgcgcgggc gtatggcgac
     6961 gacgcgggcg tggaatcact ttggcataac gccgccagaa atgccgtaag tcggattggt
     7021 cgctcgcgcc gcatccgaca ctggcagtgt gtctgatgcg acactgttcg cgtcttatca
     7081 ggccagccag agatagcgaa ccgtaggtcg gataaggcgc tcgcgccgca tccgacaaat
     7141 gtgttcagcg atagattagc tggcctgctt tttcatcata ctgataataa ataacagtgc
     7201 cgcacatagc accaccgacg gacccgccgg cgtatcgtaa aacgcggaaa aggttaaacc
     7261 gccagttact gccaccatcc ccaccaaaac agcgacacca gccatctgtt ccggcgtacg
     7321 ggcaaagcga cgcgcagtag ctgcaggaat aatcagcaac gaagtaataa tcaacgcacc
     7381 gacaaatttc atcgctacac caatcgtcaa tgccgtcacc agcatcaaca acaatttcac
     7441 ttgctgtaat ttcacaccat caacaaacgc cagatccggg ctaatcgtca tcgacagcaa
     7501 attgcgccat tgccagaaca aaatagccac cacgatgacc acgccaatcg caatagagat
     7561 gagatcttct ggcgtcactg ccagtaaatc accgaacagg taagccatca aatcaacacg
     7621 aatgttagac atcagactaa cgaccactag gcccagcgac agggcactgt gcgccataat
     7681 ccctaataac gtgtcgatcg ccagctgtgg acgcttctcc agccatacca gaccgcccgc
     7741 cagcagcagc gtaacggcaa tcaccgcata gaatggattc acgtccagca acaaaccaaa
     7801 cgcgacacca agtaatgagg catgagccag cgtatcaccg aaataagaca tacgacgcca
     7861 gactacaaac gaacccagcg gacccgcggc acaggcgagc atgatcccgg ctaaccaacc
     7921 gggaaataat aattcaatca tgagcgatca tttccccgac gcaaaacaat tcgtccctgt
     7981 aaatcgtgac gatgattatg atgatggcga tagataccca gttgttcagc accacgagga
     8041 ccaaacattg aaataaactc cggatgcagg gaaacaactt ccggtgtgcc ggaacaacaa
     8101 atgtggtgat tcaggcacag cacttcatcg gtttttgcca tcaccagatg cagatcgtga
     8161 gaaaccatta aaacgccaca atccagttca cgacgcaact ggtcaataag gtcatataac
     8221 gccacttggc catttacatc cacgccttga gtgggttcat ccagcactaa taattgcggg
     8281 caatttaaca atgctcgcgc taatagtaca cgctgcgttt cgccaccaga gagcttttgc
     8341 atcggtgcgt taatcaggtg cccggcctgg acacgtttca gtgcaggcaa aatatcttct
     8401 ttatgtgtac cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa cgtggtgtcg
     8461 agatacagct tctgcggtac atagccgatg cgcagttttc cgttgcgctt gataacccct
     8521 tcatcgggtg ttaccagccc gagcactacc cgtaccagtg tcgacttacc tgcgccattt
     8581 ggcccaagta aagtcaaaat ttttccaggt ttaagttcca gcgacacatc agagaggacg
     8641 cggcgttggc caaaagaaac cgagacattt tccagggaaa ccagacttgt catgttaatt
     8701 ttagtcttgc agtagtcatg aaatgttata atatcacact tctcatattc attacgatga
     8761 ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc agcattatcc gccgctctct
     8821 ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa cccgttgggt
     8881 tcatcgcttc tgccattgct gatggtgtaa cagaaacgga ggttttactg cctgacggcg
     8941 cttcagaaca tgattattca ctgcgtccat cggatgtaaa acgcttacag aacgcggact
     9001 tagtcgtttg ggttggcccg gagatggaag cgtttatgca aaaaccggta agcaaattac
     9061 cagaagcgaa gcaggtaacg attgcgcagc ttgaagatgt gaaaccgcta ctgatgaaaa
     9121 gtattcacgg cgatgatgat gatcacgacc acgcggaaaa aagtgacgaa gatcaccatc
     9181 acggcgattt caacatgcat ctttggcttt ccccagagat agcgcgggct acagcggttg
     9241 caatccatgg aaaattagtg gaacttatgc cgcaaagtcg agccaaactt gacgccaacc
     9301 tgaaggattt tgaggcacaa ttggcctcaa ccgaaacgca ggttggtaac gagctcgcgc
     9361 cgctcaaggg gaaaggttat ttcgtttttc acgatgctta cggctatttc gaaaaacagt
     9421 tcggactgac accgcttggt cattttaccg ttaaccctga gattcaacct ggcgcgcagc
     9481 gtttacatga aataagaaca cagttggttg agcaaaaagc aacctgcgtt tttgctgagc
     9541 cacagttcag gccagcggtc gttgagagcg tcgcacgagg gacatccgtt cgtatgggaa
     9601 cgttggatcc cctggggacg aatatcaaac tgggtaaaac aagctattca gaattcctga
     9661 gtcaattagc caaccagtat gcgagctgcc tgaaaggaga ttaatgagga agtgattacg
     9721 tgcaacagat agcccgctct gtcgccttgg cgtttaataa tttaccgcga ccacaccgcg
     9781 ttatgttggg gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg ccctatgttt
     9841 atcaccgtga tgccacgcca attgtcaaaa ccattgagct ggaacagaac gaaattcgtt
     9901 cgctcttacc tgaagccagt gagccgattg atcaagctgc acaagaagat gaagccattc
     9961 cccaggacga actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa tatgttgttt
    10021 ccactggcga tacgctaagc agcattctca atcagtatgg tattgatatg ggtgatatca
    10081 cccaacttgc tgctgccgac aaagaattgc gtaacctgaa aatcggtcaa caactctcct
    10141 ggacattaac cgcggacggc gaactgcagc gcctcacctg ggaagtgtct cgtcgtgaaa
    10201 cccgaaccta tgaccgtact gccgctaacg gttttaaaat gaccagcgaa atgcagcaag
    10261 gagagtgggt taacaatctg ctgaaaggta ccgtcggagg gagctttgtt gccagcgcca
    10321 gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat taaagccatg cagtggcaaa
    10381 tggatttccg caaactgaaa aaaggcgatg aatttgcggt attaatgtct cgagaaatgc
    10441 ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg tttgcgttca gaaggtaaag
    10501 attattacgc aatccgcgct gaggatggca aattctacga tcgtaacggt actggtctgg
    10561 cgaaaggatt cttgcgattc ccgacggcga aacagttccg tatctcgtct aactttaacc
    10621 cgcgtcgaac taatccggtg acaggtcgcg ttgcaccaca cagaggtgtt gatttcgcca
    10681 tgccacaagg tacgccagtg ctttcagtgg gtgacggtga agtggtggtt gccaaacgca
    10741 gtggcgcagc aggttattat gtggctattc gtcatggtcg cagctacacc acgcgttata
    10801 tgcacttgcg caagattctg gtgaaaccgg gacagaaggt gaaacgtggc gaccgtatcg
    10861 cgctttccgg taataccgga cgttcaactg ggccgcatct gcactatgaa gtatggataa
    10921 accagcaggc cgtaaacccg ctgacggcaa aactgccgcg taccgaaggg ctgaccggct
    10981 ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt gccgcagcta cggtttgatt
    11041 aattaacatc cattcgcagc cggtacgcag tcagtaccgg ctttttttat ttggtgcggg
    11101 gcaagttgtg ccgctacact atcaccagat tgatttttgc cttatccgaa actggaaaag
    11161 catggaaacg aaaaaaaata atagcgaata cattcctgag tttgataaat cctttcgcca
    11221 cccgcgctac tggggagcat ggctgggtgt agcagcgatg gcgggtatcg ctttaacgcc
    11281 gccaaagttc cgtgatccca ttctggcacg gctgggacgt tttgccggac gactgggaaa
    11341 aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac gtagtgaagc
    11401 tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga tggcaatgat
    11461 ggctgagttg gcaatacgcg ggccggagaa aattcagccg cgcgttggct ggcaagggct
    11521 ggagatcatc gaagagatgc ggcgtaataa cgagaaagtt atctttctgg tgccgcacgg
    11581 ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa tggcagcgat
    11641 gttccataat cagggcaacc cggtttttga ttatgtctgg aacacggtgc gtcgtcgctt
    11701 tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca ttcatccagt cggtacgtca
    11761 ggggtactgg ggatattatt tacccgatca ggatcatggc ccagagcaca gcgaatttgt
    11821 ggatttcttt gccacctata aagcgacgtt gcccgcgatt ggtcgtttga tgaaagtatg
    11881 ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc aagacgcatc gtctgacgat
    11941 tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat gatcatacga ttgagcggcg
    12001 gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca gaacaataca cctggatact
    12061 aaaattgctg aaaactcgca aaccgggcga aatccagccg tataagcgca aagatcttta
    12121 tcccatcaaa taaaaaagcc tctcgcgagg agaggccttc gcctgatgat aagttcaagt
    12181 ttgcttcaga atattcgaaa tctgttgaac tatcattgaa ctataggccg gatgtggcgt
    12241 tttcgccgca tccggcaacg tacttactct accgttaaaa tacgcgtggt attagtagaa
    12301 cccacggtac tcatcacgtc gccttgggtg acaatcacca ggtcaccaga catcaagtaa
    12361 tctttatcgc gcagcagatt aaccgcttcg ctggcagctg ctacgccgtc attagcgcta
    12421 tcaaagtgca ccggcgtaac gccacgatag agagcagtca ggttcagcgt acgttcatgg
    12481 cgcgacatgg cgaaaattgg tagaccagag ctgatacggg aggtcatcag cgcggtacga
    12541 cccgattcgg tcatggtaat gatcgccgta acgcccttca ggtggttagc tgcgtacatt
    12601 gccgacatgg caatagcttc ttccacattg tcgaactgaa cgtccagacg gtgtttagaa
    12661 acgttgatgc tcgggatttt ttccgcaccc aggcaaacgc gcgccatggc tgcaacagtt
    12721 tctgacggat actgcccagc ggcagtttct gcagacagca tcacagcgtc agtaccatcc
    12781 agaacggcgt ttgctacgtc catgacttct gcacgcgtcg gcatcgggtt agtaatcatt
    12841 gactccatca tctgggtcgc cgtgattacc gctcggttta gctgacgcgc acgacggatc
    12901 aacgctttct gaatgccgac cagttccggg tcgccaattt ccacaccgag gtcgccacgt
    12961 gcaaccatta ccacgtcaga ggcgaggatg atgtcatcca ttgcatcctg gctgcaaacg
    13021 gcttccgcac gttcaacctt ggcaacaatt ttcgcatcac atcctgcatc gcgtgccagg
    13081 cgacgggcat agttcagatc ttcgccacag cgtgggaagg agacagccag gtaatctacg
    13141 ccaatcaacg ccgcagtctt aatgtctgct ttgtcttttt cggtcagcgc ttcagccgac
    13201 aaaccgccgc caagtttgtt gataccttta ttgttggaga ggggaccacc gacggtgact
    13261 tcggtgaaca ctttcatgcc ctgaacttcc agtactttta actggacgcg accatcgtcc
    13321 agcagcagga tgtcaccagg cacgacgtca gcaggcaggc ctttgtagtc gataccgact
    13381 ttttctttgt cgccttcacc tttacccagg ttggcgtcga gcaggaattt atccccaata
    13441 ttgaggaaaa ctttgccttc tttaaaggtg gatacacgga ttttgggccc ctggaggtca
    13501 cccagaatag ccacatgacg ccccagtttt gcggcaatct cacgaacttt atccgcgcgc
    13561 attttgtgat cttcaggcga gccgtgagaa aagttcatac gtacaacgtt ggcacccgcc
    13621 gcgataactt tttcaagatt attatcgcga tctgttgctg ggcctaacgt ggtaacgatt
    13681 tttgttctgc gaagcctttt ggacatgtaa tactccgttg actgaaacaa ccaggtgttg
    13741 cttgaacatg aaatccgaat gaaataacgc cgcgatggcg ataaattaat ctcattcgga
    13801 tgtcgtataa gattaggaca gtgacagtcg cttttagcga tcgtcactta aattaagtaa
    13861 ctgcttatca aaacgcgatt ccttcagcgc ttccttgacc cgcttcaagt tatctctgaa
    13921 ttttgcaccg cgtcgcaaag taaatcctgt cgccagcaca tctatcacgg tcagctgtgc
    13981 aagtcgagaa accatgggca tataaatgtc agtatcttcc ggtacgtcga gggtaattgc
    14041 cagcgttgct tcccgggcga gcggggtacc cgcagaggtg agggcaatca ccatggcgtc
    14101 gttttcgcgt gccagctgcg ccagctcgac cagatttttt gttcttcctg tgtgagaaat
    14161 cagcaccacc acgtctccgt cgctacaatt catacaactc atgcgttgca gcacgatatc
    14221 atcggagtac accaccggaa cattaaaacg aaagaactta ttcatcgcat cgtgggcaac
    14281 ggcggcagaa gagcctaatc cgaaaaaggc gatttttttt gcctgagtga gcaagtcgac
    14341 ggcgcggttg atggcagatt tatccagtga atgacggaca tgatcgagcg ttgccattgc
    14401 ggactcaaat attttccccg tgtatgattc aacgctgtca tcttcattga cgttgcgatt
    14461 aacataggga gtgccattcg ccagactttg tgccagatga agtttaaaat caggaaaacc
    14521 gcgcgtgtcc atgctgcgac agaaacgatt caccgtcggt tcgctaacat tggcttccag
    14581 cgccagagca gcaatactcg aatggatcgc gttatcgggc gaagccagaa tgacctcggc
    14641 aactttgcgc tctgatttgc tcaaatgttc cagctgagac tggatttttt ccagcatatt
    14701 catgatgtaa agagactcac gggtaatgac gatttccgca ctgaaagaaa tcgaaatgca
    14761 gttttgtcag atattacgcc tgtgtgccgt gttaatgaca aaagcagata aaaaagttgt
    14821 tatttttttc ataacatgat cagtgtcaga tttttaccga atggaaaacg atgatttttt
    14881 tatcagtttt gccgcacttt gcgcgctttt cccgtaatcg cacgggtgga taagcgttta
    14941 cagttttcgc aagctcgtaa aagcagtaca gtgcaccgta agaaaattac aagtataccc
    15001 tggcttaagt accgggttag ttaacttaag gagaatgaca tggcggtaac gcaaacagcc
    15061 caggcctgtg acctggtcat tttcggcgcg aaaggcgacc ttgcgcgtcg taaattgctg
    15121 ccttccctgt atcaactgga aaaagtcggt cagctcaacc cggacacccg gattatcggc
    15181 gtagggcgtg ctgactggga taaagcggca tataccaaag ttgtccgcga ggcgctcgaa
    15241 actttcatga aagaaaccat tgatgaaggt ttatgggaca ccctgagtgc acgtctggat
    15301 ttttgtaatc tcgatgtcaa tgacactgct gcattcaacc gtctcggcgc gatgctggat
    15361 caaaaaaatc gtatcactat taactacttt gccatgccgc ccagcacttt tggcgcaatt
    15421 tgcaaagggc ttggcgaggc aaaactgaat gctaaaccgg cacgcgtagt catggagaaa
    15481 ccgctgggga cgtcgctggc gacctcgcag gaaatcaatg atcaggttgg cgaatacttc
    15541 gaggagtgcc aggtttaccg tatcgaccac tatcttggta aagaaacggt gctgaacctg
    15601 ttggcgctgc gttttgctaa ctccctgttt gtgaataact gggacaatcg caccattgat
    15661 catgttgaga ttaccgtggc agaagaagtg gggatcgaag ggcgctgggg ctattttgat
    15721 aaagccggtc agatgcgtga catgattcag aaccacctgc tacaaattct ctgcatgatt
    15781 gcgatgtctc cgccatctga cctgagcgca gacagcatcc gcgatgaaaa agtgaaagta
    15841 ctgaagtccc tgcgccgcat cgaccgctcc aacgtacgcg aaaaaaccgt acgcgggcaa
    15901 tatactgcgg gcttcgccca gggcaaaaaa gtgccgggat atctggaaga agagggcgcg
    15961 aacaaaagca gcaatacaga aaccttcgtg gcgatccgcg tcgacattga taactggcgc
    16021 tgggccggtg tgccattcta cctgcgtact ggtaaacgtc tgccgaccaa atgttctgaa
    16081 gtcgtggtct atttcaaaac acctgaactg aatctgttta aagagtcgtg gcaggatctg
    16141 ccgcagaata aactgactat ccgtctgcaa cctgatgaag gcgtggatat ccaggtactg
    16201 aataaagttc ctggccttga ccacaaacat aacctgcaaa tcaccaagct ggatctgagc
    16261 tattcagaaa cctttaatca gacgcatctg gcggatgcct atgaacgttt gctgctggaa
    16321 accatgcgtg gtattcaggc actgtttgta cgtcgcgacg aagtggaaga agcctggaaa
    16381 tgggtagact ccattactga ggcgtgggcg atggacaatg atgcgccgaa accgtatcag
    16441 gccggaacct ggggacccgt tgcctcggtg gcgatgatta cccgtgatgg tcgttcctgg
    16501 aatgagtttg agtaatatca gcgcttatcc tttatggtta ttttaccggt aacatgatct
    16561 tgcacagatt gtagaacaat ttttacactt tcaggcctcg tgcggattca cccacgaggc
    16621 tttttttatt acactgactg aaacgttttt gccctatgag ctccggttac aggcgtttca
    16681 ctcataaatc ctctgaatga aacgcgttgt gaatcatcct gctctgacaa ctcaatttca
    16741 ggagccttta tgaatccaca attgttacgc gtaacaaatc gaatcattga acgttcgcgc
    16801 gagactcgct ctgcttatct cgcccggata gaacaagcga aaacgtcgac cgttcatcgt
    16861 tcgcagttgg catgcggtaa cctggcacac ggtttcgctg cctgccagcc agaagacaaa
    16921 gcctctttga aaagcatgtt gcgtaacaat atcgccatca tcacctccta taacgacatg
    16981 ctctccgcgc accagcctta tgaacactat ccagaaatca ttcgtaaagc cctgcatgaa
    17041 gcgaatgcgg ttggtcaggt tgcgggcggt gttccggtga tgtgtgatgg tgtcacccag
    17101 gggcaggatg gaatggaatt gtcgctgcta agccgcgaag tgatagcgat gtctgcggcg
    17161 gtggggctgt cccataacat gtttgatggt gctctgttcc tcggtgtgtg cgacaagatt
    17221 gtcccgggtc tgacgatggc agccctgtcg tttggtcatt tgcctgcggt gtttgtgccg
    17281 tctggaccga tggcaagcgg tttgccaaat aaagaaaaag tgcgtattcg ccagctttat
    17341 gccgaaggta aagtggaccg catg
//
LOCUS       DAEBXD010000081        17173 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEBXD010000081 DAEBXD010000000
VERSION     DAEBXD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.081"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..185
                     /locus_tag="KJL94_002899"
     CDS             <1..185
                     /locus_tag="KJL94_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBD4411176.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="KJL94_002900"
     CDS             complement(210..401)
                     /locus_tag="KJL94_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBD4411177.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="KJL94_002901"
     CDS             complement(506..742)
                     /locus_tag="KJL94_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBD4411178.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KJL94_002902"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KJL94_002902"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBD4411179.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KJL94_002903"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KJL94_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KJL94_002904"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KJL94_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBD4411180.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="KJL94_002905"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="KJL94_002905"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBD4411181.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="KJL94_002906"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="KJL94_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBD4411182.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="KJL94_002907"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="KJL94_002907"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBD4411183.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="KJL94_002908"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="KJL94_002908"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBD4411184.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="KJL94_002909"
     CDS             complement(10860..12233)
                     /locus_tag="KJL94_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411185.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="KJL94_002910"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="KJL94_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBD4411186.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="KJL94_002911"
     CDS             complement(13344..13583)
                     /locus_tag="KJL94_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411187.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="KJL94_002912"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="KJL94_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBD4411188.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="KJL94_002913"
     CDS             13960..14247
                     /locus_tag="KJL94_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBD4411189.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="KJL94_002914"
     CDS             14826..14969
                     /locus_tag="KJL94_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBD4411190.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="KJL94_002915"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="KJL94_002915"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD4411191.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="KJL94_002916"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="KJL94_002916"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBD4411192.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KJL94_002917"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KJL94_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBD4411193.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4156 c         4336 g         4149 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taatcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttc ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAEBXD010000082        16105 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEBXD010000082 DAEBXD010000000
VERSION     DAEBXD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.082"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(175..1071)
                     /gene="yhaJ"
                     /locus_tag="KJL94_002918"
     CDS             complement(175..1071)
                     /gene="yhaJ"
                     /locus_tag="KJL94_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBD4411194.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTVEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1176..1877
                     /locus_tag="KJL94_002919"
     CDS             1176..1877
                     /locus_tag="KJL94_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBD4411195.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1900..2064
                     /locus_tag="KJL94_002920"
     CDS             1900..2064
                     /locus_tag="KJL94_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411196.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2276..3586)
                     /locus_tag="KJL94_002921"
     CDS             complement(2276..3586)
                     /locus_tag="KJL94_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBD4411197.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3614..4945)
                     /locus_tag="KJL94_002922"
     CDS             complement(3614..4945)
                     /locus_tag="KJL94_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBD4411198.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5220..6584)
                     /gene="tdcG"
                     /locus_tag="KJL94_002923"
     CDS             complement(5220..6584)
                     /gene="tdcG"
                     /locus_tag="KJL94_002923"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD4411199.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6656..7045)
                     /locus_tag="KJL94_002924"
     CDS             complement(6656..7045)
                     /locus_tag="KJL94_002924"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBD4411200.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7059..9353)
                     /gene="tdcE"
                     /locus_tag="KJL94_002925"
     CDS             complement(7059..9353)
                     /gene="tdcE"
                     /locus_tag="KJL94_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBD4411201.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9387..10595)
                     /gene="tdcD"
                     /locus_tag="KJL94_002926"
     CDS             complement(9387..10595)
                     /gene="tdcD"
                     /locus_tag="KJL94_002926"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBD4411202.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10621..11952)
                     /gene="tdcC"
                     /locus_tag="KJL94_002927"
     CDS             complement(10621..11952)
                     /gene="tdcC"
                     /locus_tag="KJL94_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBD4411203.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11974..12963)
                     /gene="tdcB"
                     /locus_tag="KJL94_002928"
     CDS             complement(11974..12963)
                     /gene="tdcB"
                     /locus_tag="KJL94_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBD4411204.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13062..14000)
                     /gene="tdcA"
                     /locus_tag="KJL94_002929"
     CDS             complement(13062..14000)
                     /gene="tdcA"
                     /locus_tag="KJL94_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBD4411205.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14190..14534
                     /gene="tdcR"
                     /locus_tag="KJL94_002930"
     CDS             14190..14534
                     /gene="tdcR"
                     /locus_tag="KJL94_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBD4411206.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14790..15329
                     /locus_tag="KJL94_002931"
     CDS             14790..15329
                     /locus_tag="KJL94_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411207.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15351..15698
                     /locus_tag="KJL94_002932"
     CDS             15351..15698
                     /locus_tag="KJL94_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411208.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15867..>16105
                     /locus_tag="KJL94_002933"
     CDS             15867..>16105
                     /locus_tag="KJL94_002933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411209.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKI"
BASE COUNT         4365 a         4156 c         3627 g         3957 t
ORIGIN      
        1 tgttgacaaa gtgcgctttg tttatgccgg atgcggcgta aacgccttat ccagcctaca
       61 aattgccaaa attcaatata ttgcagtgac tgcgtaggcc tgataagcgt agcgcatcag
      121 gcagttttgc gtttgtcagc agtcagaagc accttgtgaa aggtgcttaa tagattattt
      181 tccagcgaac aactttggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc
      241 acgacgccag gccataataa tatcgatctc gctggtcgat tccgggctga cgacacgcaa
      301 ccgcccttcc gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc
      361 cgccagtaat gcctgacgtt tatcttcaac cgtgctcacc gttaagcgcg gctgtttgtc
      421 cagcagctgt acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg
      481 atatttcaca cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg
      541 cgcggcaacg tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga
      601 acgaaaatgc atatccggcg cgataacgat atccgcccgc ccctgctcca gccgttccca
      661 cgcccccgcc agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt
      721 gtcgattaac gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg
      781 cgtttcccaa ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag
      841 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      901 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      961 tgcggaaggc acgcgtccca gctcatccgc tgccgccgca aaactgcccc ggcgatcgat
     1021 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
     1081 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
     1141 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
     1201 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
     1261 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
     1321 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
     1381 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggag
     1441 aggcgttgct gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag
     1501 acaaaccgtt aacgcgaatg cagctttggc tggatacctg cccgcagcga gagaatccgc
     1561 tgattcaaaa gctggcgctt aatatggaca agcagcaatt aatcgcctcg ccagagggtg
     1621 cgatgggaag cctgcaatta cgccaacaag tgtggctgca ccatatcgtg ctcgacaaag
     1681 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1741 aatttcatgc gcttacgcat catgaagaga aagcggcgct aacctgcggt gatggggcgt
     1801 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1861 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcgaccaaa
     1921 aagcgccagc cagcgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1981 ggctatgaag agatgttgag cgagctggaa gctatcgtcg cggatgctga aacgcgttta
     2041 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgtaaa cgccttatcc
     2101 ggcctacaaa acacaacaac atcaacaaat taaacacccc gtaggccgga taagatgcgc
     2161 cagcatcgta tccggcatca ggtacaaaac acaacaacat caacaagttg agcaccccgt
     2221 aggccggata aggtgcgcca gcatcgcatc cggcacaata ctcaaaacct ggcgtttatc
     2281 tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg
     2341 ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc
     2401 cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg
     2461 tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc
     2521 cgatcatact gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca
     2581 tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta
     2641 actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt
     2701 catcatccgc tccgaagtgt tctgctacaa ccaccacagg cattattgca gtgatcccct
     2761 ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg
     2821 atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct
     2881 cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct
     2941 gcgataacgc acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga
     3001 cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg
     3061 actcttgctc gccctctgcc acacacgcct ggtgggtaaa caccacacca ttgtgcgtct
     3121 caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt
     3181 tccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga
     3241 ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca
     3301 gcacttccag cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca
     3361 gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg
     3421 aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg
     3481 ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg
     3541 cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa
     3601 aatttcaggt aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga
     3661 cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca
     3721 gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc
     3781 tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga
     3841 tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt
     3901 tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca
     3961 tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc
     4021 taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt
     4081 cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa
     4141 tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt
     4201 cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca
     4261 gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac
     4321 cgacgttata cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca
     4381 gcttggtcag caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga
     4441 tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt
     4501 ccgtcacgcc gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga
     4561 cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt
     4621 tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg
     4681 tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca
     4741 aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa
     4801 tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt
     4861 ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga
     4921 cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt
     4981 tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa
     5041 taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataacg
     5101 gagatggata agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg
     5161 gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt
     5221 cagccgcaga ccactttaat ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc
     5281 atatctttgc cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc
     5341 gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta
     5401 cgttcaatgc acgggatttg tacctgtccg gcaaccggat cgcaggtcag cccaaggtta
     5461 tgctccatcg cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca
     5521 gttaaccctg ccgctgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc
     5581 gcgccagaga tggaggcgtt cattttatac agcgcgccaa tagcccccgc tgccagaaaa
     5641 tagcgggcaa ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact
     5701 gccggaataa tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg
     5761 ttttcttcac taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg
     5821 ttagagatgt tatcgctaga aaccagctga cgacgcagcg ctacggcacg acacggcaca
     5881 ttgagcggac caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc
     5941 acttgccaga tacgggcaaa ccctgcgtca atttccgctt tgctgcgcag cgccagctcg
     6001 ttgtgcatca tcaggccaga tatagacagg ccgttgtaat cacacatttt cagcagttca
     6061 cctgctgagt ggaaatcgta aggtacggac gtttcgacat cgtgcgacag gccgaagtgt
     6121 tcttcttcga caataaaccc gccgccgaca gagtaatagg ttttacttaa tagcgcttcc
     6181 tgtcccttcc aggcagtgat ccgcattccg ttctcatggc gaggcaacat ttcgggatgg
     6241 aagataatgt tctttgctac aggaaaatca acaatatgcg caccagatgc cactggcagc
     6301 cgcccgctgc gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc
     6361 ggactgtttc ctgccagtcc catgataatg gcgacatccg tggcatggcc tttgcccgtc
     6421 agtgacaacg acccgtacag atcgaccaca atatggctcg tcgcggttaa taagccgcta
     6481 ctttccagcc gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg
     6541 gagggaccaa tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat
     6601 tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt cccgctcacg aggctttacg
     6661 cactacgtac tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcaacct
     6721 gcacacagct ccgggtcggg taggtcgcct gatgctcatc gaagaactgc ttatagactt
     6781 cattgatggt ggcaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc
     6841 ccacgctcag cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgtgcct
     6901 gatcttgcac atcagccggg atctcaccgg tctgcgggca aaccggaatt tgcccggagg
     6961 tgaagaccat gctgcctaaa tcaacgccct gaacataagg gccgattgcg cctggggcac
     7021 gttgcgtttc gataatcttt ttcatacatc ctccggcgtc agagcgcctg ggtaaaagta
     7081 cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca
     7141 gagacacgga ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt
     7201 tcccgattca ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg
     7261 aaatacccat ccagcaggcc aacaaggttg gttttacgta ctggatcttc tttgcccagc
     7321 gccgcaggaa cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt
     7381 ttcgccaccg acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta
     7441 gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg gcgtattacc ggttttctgg
     7501 ccgtacacca cgttagaagt gatagtcaga atcgactggg tagggacggc gttgcgatag
     7561 gttggcagcg ctttaatttt cttcataaag cgttcaacca ggtcgcaggc aatgctgtct
     7621 acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc
     7681 aggccgtttt cgtcacggat tggtttcacg cgggcatatt tgatggcaga cagggagtcc
     7741 gtcgccaccg acaggcccgc gatgccgcat gccatagtgc gatagacatc acgatcgtgc
     7801 agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc
     7861 gcgctgatgt actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg
     7921 tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg gcccgacctg aatcttcagc
     7981 ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca
     8041 ccaaagaact gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa
     8101 tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt catactgcaa ggaagaggtg
     8161 acgatcgaca cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga
     8221 atggtcaggt taggttccgg tgccggcccc atagtgtgca gggtgtgcaa atagcggaag
     8281 gagtttttgg tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc
     8341 cagattgggt cgccggagaa cagcgaatca aattccggtg tacgcaggaa gcgtaccata
     8401 cggatcttca tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gaggacgcca
     8461 gctttaaagt cgcgctcaat gtagatatcc aggaacgatg ccgtgcgccc cagcgacatc
     8521 gcgccgccat tttgcgattt cactgccgcc agataagcga agtagagcca ctgcaccgct
     8581 tcctgtgcat tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga
     8641 atctgcaaca gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc
     8701 agatcctcgc ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgc
     8761 accagataac tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacga
     8821 ccatagccat ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt
     8881 gagtaaacat caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca
     8941 aattcactgt ccatttcacg gccataggcg tggaatgaac ttttaatcat attaatgcca
     9001 ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc
     9061 agcggctggt taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg
     9121 aaatcaaccg gcgcgtgggt tgcgttttcg atacggatgc cttccattac cttttcccac
     9181 aattccgtgg tggcaggcgt cgcttcggcg agaaaagatt catcgccttc atacggtgta
     9241 tagttatgtt gaataaaatc gcggacatta atttcgcttt tccagtccgt acctttaaag
     9301 ccaagccatg cgtcagcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat
     9361 aatctctcta caatacttca actaaattat gcaaattctg cgggcgcgtt aactttacct
     9421 aaatgaatgg catccaaagc aatcattttt tcttcgttag tcggaataac gacgcaaatg
     9481 acatgcgcat tttcactgga aacaattcgc tcgccaaaag agttagatcg attattcatt
     9541 tctgtatcaa tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaaa
     9601 cttgaattct ctcctattcc gccggtgaat ataattccat ccagccgacg taatgaagct
     9661 gcatgtccgg caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg
     9721 cgttcgtgac cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa
     9781 atacctaata atcccgactc tttattcact acgcgttcca ggtcacccag gctctggttg
     9841 gtttggctgg cgacccagga catcgcacca aagtcgacat cgccactgcg ggtacccatc
     9901 atcaagcctt ccagcggcgt cattcccatt gaggtgtcaa cactctgacc gttgcgaact
     9961 gcgcagattg acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc
    10021 agattcagca gcgaatgtgc gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca
    10081 taacggcgta cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc
    10141 ggagccatcg tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat
    10201 aattgctgcg ccgattcaat accacttaaa tttgcgtaat tatgcagggg tgccagtgga
    10261 gaaacgcgac ggatattatc aatgacttca tcggtaataa tggcggactc ggtaaaaata
    10321 ctgccgccgt gagcgatgcg gtggccaatt aaggccacgc tgtcatttaa attccgtttt
    10381 tccagttcaa atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct
    10441 ggctctcccc catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct
    10501 gacattaata cttcacaatc gctggcatcg agtacggaaa acttaatcga agacgaacca
    10561 cagttaataa ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga
    10621 ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac
    10681 gttatccaga cgaccacggt atttcgccag agacggcgct ttacggatag catacatcgg
    10741 caacaggcac agcagggatg cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat
    10801 gttcgggttg gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgagagt
    10861 attcagttta cccagcgaca ctttggtttt gtcaccttta taaccgaact tcagaatcag
    10921 accattcaag ccttccagcg tccccagata gtgaccgaag aaagatttga agatagccac
    10981 gagtgcgatg atggaagccg catattccag tgtaatcgcg aacgttgttt tcgtaccggt
    11041 catggacgca aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc
    11101 cgccatgttg gccggagaca gagtaaacag gcagctaaag gcaaagaaca tcaccactgc
    11161 aaccatcagc atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg
    11221 accgaagtct ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga
    11281 gaagttaaag gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc
    11341 atgaccggtt aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta
    11401 agggatcagc gacagagaaa tcagcaccag gctggcgata aacggccata ccaggtagct
    11461 catcacttta accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag
    11521 gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg agctggtttt cccagaacgt
    11581 cataaaggta ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa
    11641 gtacaggaac gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt
    11701 aatgttgccg gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata
    11761 aaatgcgatg gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc
    11821 tgcgcggata gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt
    11881 ccatgtggta tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc
    11941 tgaagtactc atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg
    12001 agagacgcga gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg
    12061 aatatattgg tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc
    12121 ggtgacgact ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct
    12181 gaccagcacg atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg
    12241 ggagacatca caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga
    12301 gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt acgccaataa cacgaatggt
    12361 cgggttaata gatttaattg ccaccgcaat accagcaatt aaaccaccac caccaattgg
    12421 cacaatcacg ttatcgacat catagagatc ttccataatt tccagaccaa tcgttccctg
    12481 gccagcaatc actttcggat catcgtaagg tgggataaaa atacgacctt ccatttcgac
    12541 aatttcgctc actttagcga tagtgtcgtt gaagttatca ccatgcagaa cgacttctgc
    12601 ggagtagtcg cacgttgccg ctactttgga ttttggcgca ccttttggca tcaccacttt
    12661 accgtcgata cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga
    12721 acaggccacc acgcctttgc gtttttccgc atcggtcagt gaacttaatt tattaaatgc
    12781 gccacgaatt ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc
    12841 tttgcaacgt tcactaaaat agttggagcg aggcatgcct gttttataaa ttcgcccagc
    12901 cagtcgttgt ttcgcttcaa taatatcatc aatagcaacc ggcagatcgt atgtaatatg
    12961 cattataaaa cctcttcgcc tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa
    13021 acagacaggt ggattattta tgtttaaata aaaacaaaca actaaccaac ttctattaat
    13081 tgccttcgtc tacacccatt ataagatgaa tactctttgg ctaattccac caaaaccgat
    13141 gctgcttttt taatacgata atttttcgac cataccgcgg catattgtgc cacaggtaat
    13201 gtttcttcaa ccggaatagt aataaattga ttagaaccaa aaggtgacgt catatcacaa
    13261 ggaattacag ttaagaaatc agcattgaga acaagattat aaattgtcac gactgagtcg
    13321 gttttaacga tgttttcaat actgatgcca tttctttgta acgtagtaag cagttcgctg
    13381 tagtacccca tattagtttg tggcaacacc cactgttcgt tcttcaacga ctccagcgtg
    13441 gtggtgccgg tgcatgttcg ggacttactg gctaccagca caaactcgga ctcgaacagc
    13501 ggctcaacat gtaaatcctg aagcttcatt tctgcactta acgtaccaat cgcaaaatcc
    13561 agtcgaccgt cgcggattgc cggtaagaac gaagacagtt gcgcttcata catagaaacc
    13621 tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc ctgacataaa agtaaaacca
    13681 atcaatgaag gaaaaccaaa tgagacttcc accaccgctt cagaagacat accgcttatc
    13741 tcattaacca tatttttcat ttcacgggta atggattcgg aacgggagag taacaattga
    13801 ccggcaggtg ttaatgttac accggtattt ttccgcacca ctaattccac accaaaataa
    13861 tcttcaatat cgttaatgat tttactgacg gccggttgag ttaaccctaa ttcttttgca
    13921 gccgagccga tagagccact tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc
    13981 ggaagaagaa tagtgctcat aacgacctac gttaattacc tcattgacgg catgaagtgt
    14041 atcaaaatga aataaacagg atatgtgcga ccactcacaa attaactttc attactttcc
    14101 aaggtatcgt tattacaatt aaatatcttt aaatatcaac aagttaaagc ataaaaatca
    14161 gcataaaccc tgatttttat caaaaaaaaa tgacggggat aaccatattt tatggcgata
    14221 acatcattcg ttatgtggtt aatacaaaaa aggggctgag accatatttc aagcaattac
    14281 cggataatta ccaggcaaaa tttgagttaa atttaatgag caagttctca aattttatta
    14341 taaataaacc attttcagcg ataaatactg cggcacgtca cattttttca cgctatttgt
    14401 tggagaacaa acatttattt tatcaatatt ttaaaatttc gaatacaggt attgatcatc
    14461 tcgaacaatt gattaacgtc aactttttct cttctgacag gacgtcattt tgtgaatgca
    14521 atcgttttcc ataaattctt ctcccctcat aggcgacgaa tagcattttg tgttgaggat
    14581 cacaaaacga ataattgctg gtcgccgcga taaggtcaga cgaagtcaac aagggggatt
    14641 ttcactgtgc tttacatcag cgtaggacac agaatgttgc ttcgtttact gtaacgccgg
    14701 gtaaatgagc gttttttgat agtgcgtaag tgtcgtgcga ggaaaataag catctattat
    14761 tgtttgtcat gacgacaagg caggcatcaa tgaaaggctt cccaattgca catatttttc
    14821 acccttcaat cccgccaatg cacgcagtgg ttaacaatca caatagaaat attgattatt
    14881 ggacggtaaa aagaaagttt gcagaaattg tctccaccaa tgacgttaat aaaatttaca
    14941 gtataagtaa tgaactccgg agagtattat ctgcaattac tgcattgaat ttctatcaag
    15001 gcgatgttcc ttctgtcatg atccgaatcc aaccggaaaa tatgagtcca ttcatcatag
    15061 atatttctac aggagaacat gatgattata tcatacaaac attagatgta ggcacttttg
    15121 caccttttgg tgaacaatgt acttgctcag ccgtcaataa aaaagagctg gaatgtatta
    15181 aggagacgat ttctaagtat tgcgcaaagt tcacccaaaa agaagccatc ttaacacccc
    15241 ctaggcactt taataaaacc tgcattactt cagattgttg gcaaattctc tttttttcac
    15301 ccgatcattt taataatgat ttttattgat tatttataaa ggataaaata atgttccctg
    15361 tttcatcaat tggtaatgat attagtagtg atttagtccg tcgaaaaatg aatgaccttc
    15421 ctgaaagccc cataggaaat aacctcgaag cactagcacc aggtatagaa aaactaaaac
    15481 agacctctat tcaaatggtc actttactta acgcgttaca accaagtgga aaatgcatta
    15541 tcactggtga ttttcaaaaa gaattagcgt acttacaaaa tgtaattctt tataatgact
    15601 cgtctcttcg tctggatttt ttaggttata acacccaaat tattcaacga tcggacaata
    15661 cctgcgaact taccattaat gaaccgttaa aaaactagga aatatctaca ggcaatatca
    15721 atgttaattg cccattaaaa gatatttaca atgaaatcag gaggttgaac gtaatcttta
    15781 gttgtgggac tggagatata gttgatctat cctctctgga cttacgtaat gtcgatttag
    15841 attattatga tttcacagat aaacatatgg ctaatactat tctaaatcat tttaaattgg
    15901 attcaacaaa ttttactaat gccaacatgt ttcaggttaa ttttgttagt tcaacacaaa
    15961 acgccacaat ctcctgggat tatttactaa aaataacgcc tgttttaata agcattagcg
    16021 atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg tttaaatgag cttggagaca
    16081 ttaccgaaga acaattaaaa attat
//
LOCUS       DAEBXD010000083        16009 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEBXD010000083 DAEBXD010000000
VERSION     DAEBXD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.083"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            42..373
                     /locus_tag="KJL94_002934"
                     /pseudo
     CDS             42..373
                     /locus_tag="KJL94_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            520..1881
                     /gene="yegQ"
                     /locus_tag="KJL94_002935"
     CDS             520..1881
                     /gene="yegQ"
                     /locus_tag="KJL94_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBD4411210.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2223..2540)
                     /locus_tag="KJL94_002936"
     CDS             complement(2223..2540)
                     /locus_tag="KJL94_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411211.1"
                     /translation="MKEIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            2946..3845
                     /gene="yegS"
                     /locus_tag="KJL94_002937"
     CDS             2946..3845
                     /gene="yegS"
                     /locus_tag="KJL94_002937"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBD4411212.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(3927..4700)
                     /locus_tag="KJL94_002938"
     CDS             complement(3927..4700)
                     /locus_tag="KJL94_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4411213.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4806..5846)
                     /gene="gatD"
                     /locus_tag="KJL94_002939"
     CDS             complement(4806..5846)
                     /gene="gatD"
                     /locus_tag="KJL94_002939"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD4411214.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5894..7249)
                     /locus_tag="KJL94_002940"
     CDS             complement(5894..7249)
                     /locus_tag="KJL94_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBD4411215.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(7253..7537)
                     /gene="gatB"
                     /locus_tag="KJL94_002941"
     CDS             complement(7253..7537)
                     /gene="gatB"
                     /locus_tag="KJL94_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBD4411216.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(7568..8020)
                     /gene="gatA"
                     /locus_tag="KJL94_002942"
     CDS             complement(7568..8020)
                     /gene="gatA"
                     /locus_tag="KJL94_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBD4411217.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(8030..9292)
                     /gene="gatZ"
                     /locus_tag="KJL94_002943"
     CDS             complement(8030..9292)
                     /gene="gatZ"
                     /locus_tag="KJL94_002943"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBD4411218.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            complement(9321..10175)
                     /gene="gatY"
                     /locus_tag="KJL94_002944"
     CDS             complement(9321..10175)
                     /gene="gatY"
                     /locus_tag="KJL94_002944"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBD4411219.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(10483..11535)
                     /gene="fbaB"
                     /locus_tag="KJL94_002945"
     CDS             complement(10483..11535)
                     /gene="fbaB"
                     /locus_tag="KJL94_002945"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBD4411220.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11792..13069
                     /locus_tag="KJL94_002946"
     CDS             11792..13069
                     /locus_tag="KJL94_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411221.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13066..14069
                     /locus_tag="KJL94_002947"
                     /pseudo
     CDS             13066..14069
                     /locus_tag="KJL94_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            14066..15031
                     /locus_tag="KJL94_002948"
     CDS             14066..15031
                     /locus_tag="KJL94_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBD4411222.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15005..15751)
                     /locus_tag="KJL94_002949"
     CDS             complement(15005..15751)
                     /locus_tag="KJL94_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD4411223.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(15799..>16009)
                     /locus_tag="KJL94_002950"
     CDS             complement(15799..>16009)
                     /locus_tag="KJL94_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBD4411224.1"
                     /translation="LAGYFNEYPWWVAHYYQRRPDNDGMAWRFWQHSDRGQVDGINGP
                     VDFNVFNGTGMSCRHSLMGLKKRLK"
BASE COUNT         4002 a         4133 c         3823 g         4051 t
ORIGIN      
        1 agatgctgtt ttcgataagc aatttcaatc aaggagtcat tatggctggt tggtttgaac
       61 tcagtaagag cagtgataat cagttccggt ttgtgctaaa agcgggcaat ggtgagacta
      121 tcctcaccag cgagctgtat acctcaaaag cctctgcgga aaagggcatc gcgtcggtgc
      181 gtagcaacag cccgcaagaa gagcgctatg agaaaaaacg gcaagcaacg gcaaattcta
      241 tttcaatctg aaagccgcta atcatcaaat tatcggctcc agccagatgt acgccaccgc
      301 gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc agacggtgaa
      361 agacaatacg taattacgat gccgggcgtg ggttgttggc gtccggcgct ttaccagatc
      421 ccccctacag cgctacaatg cccgccctta aagtgggggc ctcccctaac cgcttcatca
      481 ggtgaagcgg atctgacctg tcatcagaac gagagaatta tgtttaaacc ggaactcctt
      541 tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc agatgctgtt
      601 tatgcgggcc agccgcgtta ctccctacgt gtgcgcaaca acgaattcaa ccacgaaaat
      661 cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt cgtggtcaac
      721 attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc ggtggtggaa
      781 atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt gcgtgagcac
      841 ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtgaactg ggcgacggtg
      901 aaattctggc agcaaatggg actgacccgc gtgatcctct ctcgcgagct gtcgctggaa
      961 gagattgaag agatccataa tcaggtgccg gatatggaga tcgagatctt cgttcacggc
     1021 gcactgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa caagcgcgac
     1081 ccgaaccagg gcacctgcac caacgcctgc cgctgggagt acaacgtcca ggaagggaaa
     1141 gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca aaatgttgag
     1201 ccgacgctgg gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga agcccagcgt
     1261 ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat gaactcgaaa
     1321 gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca ttcgctgaaa
     1381 atcgaaggtc gtaccaaatc tttctactat tgcgcacgca ccgcacaggt ttaccgcaaa
     1441 gctatcgatg acgccgctgc gggaaaaccg ttcgatacca gcctgctgga aactctggaa
     1501 ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca cgacgattat
     1561 cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg tgagtttacc
     1621 ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc cgttggcgac
     1681 agtcttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca catggaaaac
     1741 gccaaaggcg aagctatgcc ggtcgcacca ggcgatggtt atactgtgtg gctcccggtc
     1801 ccgcaggatc ttgagctaaa ttacgcgctg ctaatgcgta atttctccgg ggaaaccacg
     1861 cgtaaccccc acggtaagtg attaatttcg attatttttc ccggatggaa aattcttaga
     1921 aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga aaaacataat
     1981 ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct cccttacacg
     2041 ggcttatttt ttgtacataa gctgtaaaga aggattgatc cctgataacc gtcttccatt
     2101 gaccacatcg atagaatccc ccttcatagc acaatgcctt tcacgtaacg gtatcgtgct
     2161 cgcccaggtt ccggctacgc acagccagaa cgcgcatgtt tgacgcttac aaaaaaatat
     2221 tctcactctc cacatttgaa tgtcagacga gcgacgccat gtaatcctgc accttctgtc
     2281 ttcaggtcaa ctatctgcat ttttttgccc tgagtaacac agaaatgggc tgcatcattt
     2341 tttactatat tttctgcacc agatattctg cccctggcta aagaagcttc ggcttcggtg
     2401 tagtattggt tatcgagttt acgctgaata ttacttttat atgcaagacc aaatttaccg
     2461 atacttgttt catcattatg cacagcacaa ccagatataa gaaaaatact aattaatgat
     2521 atagcagcta tctctttcat ctcaccttcc cccattaaat accaacgaca ctctctagtg
     2581 tttaaatata ataatggcat gattattata attgaatagg attataataa atgctctgta
     2641 caacatttcc tacataagta ggaattacgg acattgaggc ccttcagggt aactccatgg
     2701 gggctttaat atattatatt gaagatgcca ctgtttagtt gaatattagg tatatgctct
     2761 tttttgaaat ttatcggtgg cagccgttag tattcgctgt ccccattgca agctcctggt
     2821 ggtaaccact gaatcctcca tacttgaact gactttttat cctccgactt tcatcctgtt
     2881 ctgactccac cttttgtttt ctgctctaca ctatctacag accaatcata aaggcacata
     2941 cgatcatggc agaatttccc gccagcttac tgattcttaa tggtaaaagt actgacaatc
     3001 tacccttgcg cgaagcaatt atgctgttgc gtgaggaagg aatgacgatc catgtgcggg
     3061 tcacctggga gaaaggtgat gccgcacgat ttgtagagga ggcccggaag ttgggcgtcg
     3121 caacggtgat tgccggtggt ggcgatggca ccattaatga agtttctacg gcgttgattc
     3181 agtgtgaggg ggatgacata cccgcgctgg gaattttgcc attaggaacc gccaatgatt
     3241 ttgccaccag tgtagggatt cctgaggcac tggataaggc gctgaaactg gcaattgccg
     3301 gtaatgccat tgcgatagat atggcgcagg tcaacaaaca aacctgtttt attaatatgg
     3361 cgacaggcgg atttgggacg cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc
     3421 tgggtggcgt ctcttacatc attcatggct taatgcgcat ggatactctg caaccggacc
     3481 gttgtgaaat ccgcggtgaa aactttcact ggcaaggtga cgccctggtc attggtattg
     3541 gtaacgggcg tcaggccggt ggcggtcaac aattgtgccc gaacgcgtta attaacgatg
     3601 gcttgctgca actgcgcatt tttaccggcg atgaaattct tccggctctc gtatcaacct
     3661 taaaatctga cgaagataac ccgaatatta tcgaaggcgc ttcgtcgtgg tttgatatac
     3721 aagccccaca cgaaatcact tttaatcttg atggcgaacc gttgagtggg caaaacttcc
     3781 atattgaaat acttccggcg gcgttgcgtt gtcgattacc accagattgt ccattattgc
     3841 gttaatcaaa ctattctctc ttgaaccagg gagaatccgt aatgccggat gcggtgtaaa
     3901 caccttatcc ggcatacaga acaatactac accgtaatta attttacccg tgtcttctgc
     3961 aatgccaatt tatccacctc tgacacagca tcatcggtaa caataatgtc aattttttcg
     4021 attggcagta cctgattaaa accgcgacga ttaaatttcg atgaatcgag aacagcaatc
     4081 actttatttg cggcggtgac catcgccccg ctaatcgaat aaccttcatt aaaggtcgta
     4141 ataccattaa ccgcatcaat accatcagca ccgacaaaca ttaaatcggc attaatatcc
     4201 tgcaatgaac gctcggcaat ggaaccatgc atcgagcgcg ttttatggcg gaccgtgcca
     4261 ccacagacga ccagagtaat gtctttattt tcggaaaggg caaacgctgc cgggagactg
     4321 ttggtaatca ccgtgatatt tttagcggtc attaatcctt cggcgataag cattgtggta
     4381 cttccgctgt cgagaataac ggtcatccct tcatggatca ttttgactgc cgcctgcgct
     4441 attctgtttt ttggcgcgct ggcgagctga aatcgctctt taaacccgac ttcctgggtc
     4501 tcggtttcac tattaccgga cattattttc gccgcaccgc catgaaagcg cgtaaccacg
     4561 cctttttgtt cgagaaagcg caaatcggca cggattgtcg cttccgaggc agcaaatact
     4621 cccgccagat cctgaacaag cacggttccc tgttcattca ctaattggat gatcttattc
     4681 cttcgctcga atgaattcat cgtcatttac ccatattcaa ttgtggctag tgtaaacgaa
     4741 gggggaccat tggtgaatga tctgttttgc acaataccgg gccagcatca cgctggcccg
     4801 cggtttcatg gaatgagcaa cactttgccc ggcatggcat tacgagcgat gtcacgcacc
     4861 gcctgggtga agctttcaaa gcttccacgg tgagcgatta atggctccag gcttaactta
     4921 cgttctgtca gcaaccggct cgccgtttcc cactcctgcc ccggccaagg gctggaatag
     4981 ttcatccaac tgccgataac cgtcagctct ttacgcaata ttttgccaaa cgttgccgat
     5041 gttaaatgca gatcctgatg caacgtgccc accagcgcca gttgggcatg aggaccggca
     5101 atctctaccg ccagttcgac agtttgcggt acgccagccg tctcgaggat aagctgatta
     5161 aagcgcagtt cgcgtaaaac gctctgcatt tgcggcgcgc tcatttcgct actgttaaat
     5221 gtttgcatcg caccgaaaga ttttgccagt gccagttttt ctgagctaat gtcgatcgcc
     5281 gtcacactct ttgctcccag cgcgacagcg cactgaatag ccagcaggcc aatggttccg
     5341 gcaccaataa taataacgtt tttattctca caaccttgcg ctaaatgaaa agcatgcagg
     5401 ccaacggtaa tcggctcaat aaaagcccca tcctcaatag gcatatccgt gggtagagca
     5461 aagacatttt ttcgcttaac gacaatatat tcagcaaatc caccatcacg ccgcgagcca
     5521 ataaaatcat attttgcgca ctgggaataa aaccctttca aacactctgg acaagtaaaa
     5581 cagggtaata acggcacaca ggcaaccgca tcgccagggt gtaaatcatc aacaccggat
     5641 cccaccgcat caatatagcc gctaaattca tggcctaacg ttattggata ataatgtgca
     5701 ccatttttaa atatcctggg taaatcggaa ccacataagc ccgagctggc aatttttacc
     5761 cgcacctcat cctgatgttt aatttcagga atgacgcttt ttgcaacgcg cacgatacca
     5821 tcagtatcat tcactactga tttcataaaa actcctgatt gttaaggggg ataacctccc
     5881 cctcaggtaa aaattattct gcgagaacgg ctttctcttg tttagtaaaa ccacgcgctc
     5941 tacgccaggt catgaaaata ccggtcagat aaattgcgcc gataataatg aaaccgggaa
     6001 tattttgcgg ggagaaaacc tgaatcagta accaggtaat gggagaaccg ccctgatcca
     6061 ttgaagccac catacccccg gcttttaacg ccccagcatt agccgccggt tgggtgtgca
     6121 aaccaatagt ttgcgtcgcg atccacaggg tgatgctcat aatgatgaca cccgagatta
     6181 aggtgcggaa cagatttcca cgatgcaccg cgaccgccat cgccacgaag aagccgatag
     6241 tggcaagatc gccaaacggc agtacctgat tacccggaac acaaacagca attaaaatgg
     6301 tgagcggaat aaaaatcagg cttgccgata ccaccgccgt atgccccagc agtaatgcgg
     6361 gatccaggcc aatcaggaac tcctgaccgc cgaacttcgc ctgtaaacga ctacgggcct
     6421 gcttagcgat gggcgttaaa ccatccatga tgggtttaat catccgtggc atcagcagca
     6481 taactgccgc cgtttttacc gccagctgca atacaccttt gacatcgtaa cccgcgagga
     6541 tgccgataat cagccccatc acaaaaccca cggtgacagg ttcgccaaaa gggccaaatt
     6601 tgcgctgaat atcgtcggcg ctaaatttaa ttcggttaac gcctgggatt ttctcgatga
     6661 tagcatcgac cagcaccgca atcggcccca tatacgccga cgtaccgtgc ggaatagcaa
     6721 taccttccag ctcaaagaaa tttcgggtat cgcgggcaaa ccagtcgcag agcttataaa
     6781 caaacgccgc gtgaattacc acacccgcca tccctatcat ccatgaaccg gttgccaggt
     6841 gcagcaacgc gccggtgaag gtcatatgcc agatattcca gatatcaaca tttaccaccc
     6901 gcgtcatacg ggtcagtaac atcgccacgt taaccaatat cgcaatcgga atcgccacca
     6961 gcgcaatttg cgacgcccag gtcattggtg aagagcccgg ccagccgaca tcgaccacat
     7021 gcagattcag gtcgaaattt tccgccatcg ctttcgccgc cggaccaatg gaatccagca
     7081 ttaagccaat cacaaggcca atgccaacaa agccaatccc gatatgcaga cccgctttaa
     7141 agcaatcgcc tgccttcatg cctaatattt tagaaaaaat aatgatgaca accggcagca
     7201 tcactgtagg gccgaggtcg agaatataac gcatgacttc tgaaaacata ggtcacccct
     7261 gtaagatagt cagaatttta ttttgtaatg cttcgatacc gacaccagaa acaaaaggca
     7321 tgccgtgaac taacggaata tcgccaaaac tacgatccac cctggcagtg gtacatatca
     7381 aatgcacacc atccatatag gtttctattt cattaacccg acactggatt aattcaacag
     7441 gaatattatg actctgacac aactctttaa tttcttccgc cgccatcgta gaggtcgcaa
     7501 ccgcgcctcc gcaagcgaca ataatcttgc gtttcataaa tagtactttt tattgttaca
     7561 gagggattta ttcgtctgaa tctaaaacat actttgtgaa gtattccttt aactgagttt
     7621 caggaagagt aattagtgtc tcgacgatat cgggctgttg taacttgcca aataaacagc
     7681 gtaaaagttt caattgctgc tgcggatttt ccacaatcaa cgcaataacc aacgataccg
     7741 ccacgtcgtt atcatcatcc gcttgctgaa aatgaacttt atttgttggc cttaacagat
     7801 aaatggctga cgacttagca tgaatcgcct cacaatgcgg tattgcaatg gcgtgctgct
     7861 caagcattat cccggtaggg aattctgctt ctctggcaat taatgcctgt ggccaggtat
     7921 catgaaccac acctttcgcg agcatctcat taccgatata ggttaaaact tcgctacgat
     7981 cgacaaaaga aattccgcta cgaacaaaca ggttagtcat ataccgtcct tattccgcac
     8041 agccgtagcg ataggcgcgc aaaacatcat aaattttatt cataataagc tgatgcggca
     8101 ttgctgataa ttccccggac tgaatgcgtt caaattgttt gggaagatac tgactaatca
     8161 tgcccagtgg gatgtccacg ccttgcagat tcaccatcat cgtttcaacg ctatttttaa
     8221 tccgactatg cggccagtaa taacgaatac gatccgacag gctgtaacga atatccagta
     8281 atgaatcgtt aaaacccgtg cgataatatt ttttccagta ttgcggttcg tcgagcatca
     8341 cttcttcaat taccgccagg caaccgctgc gattttcggg ggcgataagt tcctgctcaa
     8401 tttgtgccag tgcaaatatc gcctcgcgta aagcaaaggt taatgcggga ccgactttca
     8461 atattgcaaa gtgatcgcgg actaattccc aataagccgt ccgggtctgg taatcggtag
     8521 aatgtgcttc ataaaccatt cgggtgtttt ctatccattg cgccagcgcc tgcgcttcct
     8581 gcggctgata atggataata ttgctgtgat caaattccac acccggctgc accacgatgg
     8641 caatcacacg tgttaacgcc tctgtcagcc cacgggcaat aaaggccttt tgatgcgtac
     8701 gtaaagtatt ggcggcatct tcaacatggg tgatgtgtac tgactgaatg gcgctggcct
     8761 caccgcccgg aaccggaact tcagtgccaa tgacatagct cagttgctca cgctggcaat
     8821 ctgtcgccac actttccgca gcaaagcaaa gcacagcagc tcgttccgca accgtttctg
     8881 gtgctaacgg tatggtatcc cccgcgcagg acattgacgc atcaagatga attttactga
     8941 agccagcacg aacatatgct tttaccagct cgacggattt ttccatcgcc gcatccgcat
     9001 tttcttgctg ccagcagttt ggtcccagat gatcgccgcc gagaataatg cgttcgcgtg
     9061 caaacccaac tttatcggca atcgtaaaaa caaattcgcg aaagtctgcc ggtgtcattc
     9121 cggtataacc gccaaattga ttgacctggt ttgacgttgc ttcaatcagc actttgcgcg
     9181 tgctgttgcg atcaaatgcc agcgccgctt cgataaccaa cggatgggca gaacagactg
     9241 aacatatgcc gatatgttca ccagctttat gccgggcaat taacgttttc atgttttttc
     9301 cttgttaaat ggcaggtgcg ttatgccctg ccctcgcagc cacaatcggc aatcactttg
     9361 ctcaccacat cgcgcattgc ggatttagcc gactgcaaat aatcccgggg atcggtcgct
     9421 tcggggtgct cggtcaggta attttttaac gactgcaaga aggcattttt cagctccgtt
     9481 gcaacgttga ttttgcatat ccccagtttg atggtttgct gaatatcctt agtcgataat
     9541 cctgacgcgc catgcagcac cagcggtaag ttcacccact ggcgaatgtt ctccagtcga
     9601 gaaaaatcaa gcaccggtgc gctggcatac ataccgtgag ccgtgccgat ggcgaccgcc
     9661 agggaatcaa tcccggttgc ctcggcaaat tcacgcgcct gtgcggggtt ggtgtacaac
     9721 gcatcgactt cattgacttg cacatcatct tcctggccgc caagttgccc cagctccgcc
     9781 tcgacgctga catcaaagcg atggcaaaaa tccaccacct ctttgacccg tgaaatattt
     9841 tgcgcaaaag gcaaatgcga ggcgtcaatc atgactgagc gcacgccaga acgaaccttc
     9901 tgagcgatat cgtcaaattt cgtgtgatgg tcgagatgaa ttgccagtgg atggtgatat
     9961 tgcttcgcca tcgcgctgac cagcgccaac agattttctg taccagcatg agtaaatgtg
    10021 ccaggcgttc cggcgatgat gaccggcgca tgcaggttgg cagcggtttc caccaccact
    10081 tgcatcgttt cgagattgtg aatattgaat gccggaaccg cataaccgcc gcgctgtgcg
    10141 ttgttcagca tctgctttgt cgataccacg tacattttca tatcttgtcg tttgctttcg
    10201 atttcaaaat ataatgaaat tatttgcttt taaatgtcga gataatgatc acaaaaacaa
    10261 caatatgaaa attgttcgag gagtgaaagg caaaaaaacg gcctcccgat agggaagccg
    10321 tagcaaagtg cgcgtgtttt tatgccggat gcggtgtaaa catacgtggc agtgctatgt
    10381 aggcatgata agacgcttcc gcgtcgcatc aggcataggt tgccggatgc agtgtaaacg
    10441 tcttatccgt cctacggggt ggtgctgttc gtatgaggcg tatcaggcaa tagtaatttt
    10501 gctatcgaga taaacgtcct gcacggcatt aatcagtttc acgccgtcag ccatcgattt
    10561 cttgaacgcc ttacgtccaa gaatcagccc cattccgcct gcgcgtttgt tgataaccgc
    10621 agtacgcact gcatcgctga ggtcagtttc accgcctgca gcaccgccgg agtttatcaa
    10681 cccagcccgg cccatatagc agttagctaa ctgataacgc accagatcaa tcgggttttc
    10741 gctggttaac ttgctgtaca cgcgatcgtc ggtataaccg taattaatcg ctttatagcc
    10801 gccgttattt tccgccattt tttgtttgac gatatctgca cctatggttg ccgccagatg
    10861 gttcgcctga ccagtcaggt cggcggaaac atggtaatca acgccatctt tcttaaaggc
    10921 ggagtttcgc aaataggccc acagcactgt caccatgccc agctcgtgcg cgcgttcaaa
    10981 ggccgcagaa atttcttcaa tctggcgacg tgactcttcc gagccaaaat agatagtcgc
    11041 accaaccgcc accgcgccca tgttgaaggc ctgctccacg ctggcataca gcgtttgatc
    11101 gtaggtattc gggtaactta gcgtctcgtt gtgattaagt ttgacgagaa atggaatgcg
    11161 atgcgcatag cgccgcgata ccgacaccaa cacgccgtaa gttgatgcca cacagttaca
    11221 gcccgcttcg atcgccagtt caacaatgtt tttcgggtca aagtagagcg ggttagcagc
    11281 aaatgaagct ccggcagagt gctcaacgcc ctggtcaacc ggcagaatag aaagatatcc
    11341 tgtgccagcc agacgcccgg tgttgtacaa cgtctgcata ttacgtaaca ccgctggcgg
    11401 gcgattattg tcaatcatca cgcggtctac gtagtcatgt ccggggagat aaagctggtc
    11461 agaaggaata gtcatacaac ggtgctgtaa aaggttgtcg gcgtctttgc caagcaactg
    11521 cgcaatatct gtcattacta tgctcccgta aattccgatt ggatatcggc tatggattgt
    11581 cctggcccgc cttttgcggg caatcataat cctagtcgtt acgggcgagt ttttccatct
    11641 tttggatgtt ttttcagcgt tttctgttgg ctcgattcat cagaaaaaat gttagcgcgg
    11701 tcaagttttc accgcaaagg tattcaaaag gcattattat gtggtattgt catcgcgtac
    11761 cttacattac ctgtcatgaa ggaattaaaa gatgaaaaca acagcaaagc tgtcgttcat
    11821 gatgtttgtt gaatggttta tctggggcgc gtggtttgtg ccattgtggt tgtggttaag
    11881 taaaagcggt tttagtgccg gagaaattgg ctggtcgtat gcctgcacag ccattgcggc
    11941 gatcctgtcg ccaattctgg ttggctccat cactgaccgc tttttctcgg cgcagaaagt
    12001 gctggcggta ttgatgttcg ctggtgctgt gctgatgtat ttcgctgcgc aacagaccac
    12061 ttttgccggg ttctttccgt tactgctggc ctactcgcta acctatatgc cgaccattgc
    12121 gctgactaac agcatcgctt ttgccaacgt gccggatgtg gagcgtgatt tcccacgcat
    12181 tcgtgtgatg ggcactatcg gctggattgc ctccggtctg gcatgtggtt tcttgccgca
    12241 aatgctgggg tatgccgata tctcaccgac taacatcccg ctgctgatta ccgccggaag
    12301 ttctgctctg ctcggtgtgt ttgcgttttt cctgcccgac acgccgccaa aaagcaccgg
    12361 caaaatggac attaaagtca tgctcggcct ggatgcgctg atcctgctgc gcgataaaaa
    12421 cttcctcgtc tttttcttct gttcattcct gtttgcgatg ccactggcgt tctattacat
    12481 ctttgccaac ggttatctga ccgaagttgg catgaaaaac gccaccggct ggatgacgct
    12541 cggccagttc tctgaaatct tctttatgct ggcattgccg tttttcacta aacgctttgg
    12601 tatcaaaaag gtattattgc ttggtctggt caccgctgcg atctgctatg gcttctttat
    12661 ttacggtagt gcggatgaat atttcaccta cgcgttactg ttcctcggta ttttgcttca
    12721 cggcgtaagt tacgattttt actacgttac cgcttacatc tatgtcgata aaaaagcccc
    12781 cgtgcatatg cgtaccgctg cgcaggggct gatcacgctc tgctgccagg gcttcggcag
    12841 tttgctcggc tatcgtcttg gcggtgtgat gatggaaaag atgttcgctt atcaggaacc
    12901 ggtaaacgga ctgactttca actggtccgg gatgtggact ttcggcgcgg tgatgattgc
    12961 cattatcgcc gtgctgttca tgattttttt ccgcgaatcc gacaacgaaa ttacggctat
    13021 caaggtcgat gatcgagata ttgcgttgac acaaggggaa gttaaatgaa aacagaacgt
    13081 attctcggtg ctctttatgg gcaggcgtta ggggatgcga tggggatgcc ctccgagctt
    13141 tggccacgca gccgcgttaa agcacacttt ggctggattg accgttttct gcctggacca
    13201 aaggagaata acgcggcctg ttattttaac cgcgccgaat tcaccgacga tacctcgatg
    13261 gcctgtgtct ggcggatgcg ttactggaat gtgaaggcaa gatcgatccg aatctgattg
    13321 ggcgtaatat tctcgactgg gcgttgcgtt tcgacgcctt taacaaaaac gtactaggtc
    13381 cgacctcgaa gattgcgctt aacgccattc gcgacggtaa acccgttgct gaactggaaa
    13441 acaacggcgt aaccaacggc gcagcgatgc gcgtctcgcc attaggttgt ttgcttccag
    13501 cgcgtgatgt tgattccttt attgatgatg tggcgctggc gtccagcccg acccataaat
    13561 ccgatctggc ggttgcaggc gcggtagtca tcgcatgggc gatttctcgt gccattgacg
    13621 gagaacgctg gtcagcgatt gttgattcat tgccttcaat tgcgcgacat gcacagcaaa
    13681 aacgcaccac cacgttcagc gcctcactgg cggcacgtct ggagattgcg ctgaaaattg
    13741 tgcgtaatgc cgacggcacc gaatccgcca gcgaacagct ttaccaggtc gttggcgcag
    13801 gcaccagcac tattgagtcc gttccgtgcg ccattgcgct agttgaactg gcacaaaccg
    13861 acccaaaccg ctgcgccgtc ctgtgcgcca atcttggcgg cgacacagac accataggtg
    13921 ctatggcgac ggcgatttgc ggcgcgttgc atggcattaa cgctgtcgat cctgcgttaa
    13981 aggcggagct ggatgcggta aatcagcttg atttcaaccg ctatgccaca gcgctggcga
    14041 aatatcgtca acaacgggag gcggtatgag cggcgctcga ttacacacac tgctgcctga
    14101 attaaccacg cgtcagccgg tgatggtcgt cggcgcggcg gtcattgacg tgatcgccga
    14161 cgcttatgcc ctcccctggc gtgggtgcga tatcgaactg aaacagcaga gcgttaacgt
    14221 tggcggctgc gcactgaata ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa
    14281 tgccttgccg ctcggtcagg gcgtgtgggc ggagattatt cgcaaccgga tggcgaaaga
    14341 ggggttaatc agcctgatcg ataacgccga aagcgataac ggctggtgtc tggcactggt
    14401 tgagccggat ggcgaacgca cttttatgtc attcagcggt gtggaaaatc aatggaatcg
    14461 ccaatggttg gcacgattaa ccgttgcgcc tggcaacctg ctctattttt ccggttatca
    14521 actggcctcg ccctgcggcg aattgttagt ggaatggctg gaaaagctgc aagacgtgac
    14581 gccgtttatc gatttttgcc cacgtattgg cgatatcccg gacgcattac tggcgcggat
    14641 catggcctgt cgacctttag tgtcactcaa tcgtcaagag gctgagattg ccgccgaacg
    14701 ttttgcttta tccgcagaga taacaacact tggcaagcaa tggcaggaga aatttgccgc
    14761 gccgttgatc gttcgtctcg ataaagaagg cgcatggtat ttcagcaacg acgcttctgg
    14821 ctgcattccg gcatttccaa cgcaagttgt agacaccatt ggggcgggcg acagtcatgc
    14881 aggtggcgta cttgccgggc tggcctctgg tctgccactg gcggatgccg tattactggg
    14941 caatgcagtg gcgtcgtggg ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga
    15001 ggaactactc ctcgcacaca aaaacgtata gatcgctgcg acagtggcta atgctgtact
    15061 caataggccg ttgctgttgg tcaagcgcca cttgcttgat caccagcacc ggtattttgc
    15121 tatctaactg aatatgtgac tgaaactcgg catccggcat ccgggcgcta acgcgggaac
    15181 gcgtacgttg tgggtaaatg tgctggctgc ggaaatagtc ataaagagaa attccgatgg
    15241 catcaacatc atgaattaaa tgcgccggaa cccacgattc ctcaatcgat accgcttctt
    15301 catcgacata gcgaatacgc ttaagcaaaa agacatcact tcccgcttcg accgccagct
    15361 gctgcgcaac ttcttcggga catttcacga cacgcttatt aacccatagc gtatcgggct
    15421 ttttcccgca caataccacc tgctgagaaa aacctctcgc ttctttcaac gagtattcga
    15481 agatgttgtt gatctgcgtg ccgtaaccgc gcgaacgcgt caccacgccc tcttcttcca
    15541 gcgcctgcat cgccttgcgc accgtaatgc gcgaaacgcc ggttaactga cttaaatcac
    15601 gctcaccggg caaaatattg ccatgctcca gcaccccgct gcgcacggca ttttttaccg
    15661 tttcggcaaa cttaatataa agcggcgtgt tatccgccgc taaaatacgc tcgttcagtt
    15721 gggcaatcag ctgggtatgc gcttgttcca tttgtctttt tcctgccagg gagtctggtg
    15781 ggagtatatc actgcttctt atttaaggcg tttctttaat cccatcaacg aatgcctgca
    15841 actcatcccc gtgccattaa acacattaaa atccaccgga ccattgatgc catctacctg
    15901 tccacggtcg gaatgctgcc agaagcgcca ggccatgccg tcattgtccg gacgacgttg
    15961 ataatagtga gccacccacc acggatattc attgaaatag cccgccaga
//
LOCUS       DAEBXD010000084        15544 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEBXD010000084 DAEBXD010000000
VERSION     DAEBXD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15544
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.084"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KJL94_002951"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KJL94_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBD4411225.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="KJL94_002952"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="KJL94_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBD4411226.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="KJL94_002953"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="KJL94_002953"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBD4411227.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="KJL94_002954"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="KJL94_002954"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBD4411228.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="KJL94_002955"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="KJL94_002955"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBD4411229.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="KJL94_002956"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="KJL94_002956"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBD4411230.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="KJL94_002957"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="KJL94_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBD4411231.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="KJL94_002958"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="KJL94_002958"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBD4411232.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="KJL94_002959"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="KJL94_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBD4411233.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="KJL94_002960"
     CDS             7138..7749
                     /locus_tag="KJL94_002960"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBD4411234.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KJL94_002961"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KJL94_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBD4411235.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KJL94_002962"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KJL94_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KJL94_002963"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KJL94_002963"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBD4411236.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="KJL94_002964"
     CDS             complement(11943..12302)
                     /locus_tag="KJL94_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411237.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="KJL94_002965"
     CDS             complement(12299..12805)
                     /locus_tag="KJL94_002965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411238.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="KJL94_002966"
     CDS             complement(12951..13514)
                     /locus_tag="KJL94_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411239.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="KJL94_002967"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="KJL94_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="KJL94_002968"
     CDS             complement(14722..15096)
                     /locus_tag="KJL94_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBD4411240.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..>15544)
                     /gene="wzy"
                     /locus_tag="KJL94_002969"
     CDS             complement(15100..>15544)
                     /gene="wzy"
                     /locus_tag="KJL94_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBD4411241.1"
                     /translation="GSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLFGRG
                     FGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSLILF
                     FSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
BASE COUNT         4361 a         3734 c         3532 g         3917 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gccc
//
LOCUS       DAEBXD010000085        15400 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEBXD010000085 DAEBXD010000000
VERSION     DAEBXD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.085"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..1175)
                     /locus_tag="KJL94_002970"
     CDS             complement(<1..1175)
                     /locus_tag="KJL94_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD4411242.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="KJL94_002971"
     tRNA            complement(1374..1458)
                     /locus_tag="KJL94_002971"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="KJL94_002972"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="KJL94_002972"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBD4411243.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="KJL94_002973"
     CDS             2802..4304
                     /locus_tag="KJL94_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBD4411244.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="KJL94_002974"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="KJL94_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD4411245.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="KJL94_002975"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="KJL94_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD4411246.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="KJL94_002976"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="KJL94_002976"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBD4411247.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="KJL94_002977"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="KJL94_002977"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBD4411248.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="KJL94_002978"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="KJL94_002978"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBD4411249.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="KJL94_002979"
     CDS             complement(11869..13065)
                     /locus_tag="KJL94_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBD4411250.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="KJL94_002980"
     CDS             13258..13761
                     /locus_tag="KJL94_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4411251.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="KJL94_002981"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="KJL94_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBD4411252.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="KJL94_002982"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="KJL94_002982"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBD4411253.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3972 a         3930 c         3966 g         3532 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatca ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaaa gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca tacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 agcgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata
//
LOCUS       DAEBXD010000086        15098 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEBXD010000086 DAEBXD010000000
VERSION     DAEBXD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.086"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="KJL94_002983"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="KJL94_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBD4411254.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="KJL94_002984"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="KJL94_002984"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBD4411255.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="KJL94_002985"
     CDS             1531..2478
                     /locus_tag="KJL94_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBD4411256.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="KJL94_002986"
     CDS             2548..3354
                     /locus_tag="KJL94_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4411257.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="KJL94_002987"
                     /pseudo
     CDS             3364..3771
                     /locus_tag="KJL94_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3771..4265
                     /locus_tag="KJL94_002988"
     CDS             3771..4265
                     /locus_tag="KJL94_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBD4411258.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="KJL94_002989"
     CDS             4331..5128
                     /locus_tag="KJL94_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBD4411259.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="KJL94_002990"
     CDS             5139..5927
                     /locus_tag="KJL94_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBD4411260.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="KJL94_002991"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="KJL94_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBD4411261.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="KJL94_002992"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="KJL94_002992"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBD4411262.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="KJL94_002993"
     CDS             complement(8370..10001)
                     /locus_tag="KJL94_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBD4411263.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="KJL94_002994"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="KJL94_002994"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBD4411264.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="KJL94_002995"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="KJL94_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBD4411265.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3936 g         3589 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatagcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggagaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtagaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca tggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct agtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc gcacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gcggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gtacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattaa gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 ctcaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg tcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttca
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaa
//
LOCUS       DAEBXD010000087        15030 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEBXD010000087 DAEBXD010000000
VERSION     DAEBXD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.087"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..236
                     /locus_tag="KJL94_002996"
     CDS             <1..236
                     /locus_tag="KJL94_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBD4411266.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="KJL94_002997"
     CDS             246..884
                     /locus_tag="KJL94_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBD4411267.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="KJL94_002998"
     CDS             887..2050
                     /locus_tag="KJL94_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD4411268.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3759
                     /locus_tag="KJL94_002999"
     CDS             2134..3759
                     /locus_tag="KJL94_002999"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBD4411269.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="KJL94_003000"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="KJL94_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBD4411270.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="KJL94_003001"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="KJL94_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBD4411271.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="KJL94_003002"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="KJL94_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD4411272.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="KJL94_003003"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="KJL94_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBD4411273.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="KJL94_003004"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="KJL94_003004"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBD4411274.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="KJL94_003005"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="KJL94_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBD4411275.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="KJL94_003006"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="KJL94_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBD4411276.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="KJL94_003007"
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="KJL94_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBD4411277.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="KJL94_003008"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="KJL94_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBD4411278.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="KJL94_003009"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="KJL94_003009"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBD4411279.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSRERRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="KJL94_003010"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="KJL94_003010"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBD4411280.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /locus_tag="KJL94_003011"
     CDS             complement(12356..12631)
                     /locus_tag="KJL94_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4411281.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="KJL94_003012"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="KJL94_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBD4411282.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="KJL94_003013"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="KJL94_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBD4411283.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="KJL94_003014"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="KJL94_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBD4411284.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="KJL94_003015"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="KJL94_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBD4411285.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..>15030)
                     /locus_tag="KJL94_003016"
     CDS             complement(14468..>15030)
                     /locus_tag="KJL94_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD4411286.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3510 a         3666 c         4051 g         3803 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cattttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccacccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac tcatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgtatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac tattccttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgtg
     7621 gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa ataattgttt
     7681 cgttgtgtga atcacactct atccgtgtgg aattatttgc gggtcgcgcc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtacacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca
     9901 cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgaac ggcgtctggc gctggtgaat gctattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgtgctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg gaaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgtta
//
LOCUS       DAEBXD010000088        14854 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEBXD010000088 DAEBXD010000000
VERSION     DAEBXD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.088"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KJL94_003017"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KJL94_003017"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBD4411287.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KJL94_003018"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KJL94_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBD4411288.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KJL94_003019"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KJL94_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBD4411289.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="KJL94_003020"
     CDS             2977..3060
                     /locus_tag="KJL94_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBD4411290.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KJL94_003021"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KJL94_003021"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBD4411291.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KJL94_003022"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KJL94_003022"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBD4411292.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="KJL94_003023"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="KJL94_003023"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBD4411293.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="KJL94_003024"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="KJL94_003024"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBD4411294.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KJL94_003025"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KJL94_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KJL94_003026"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KJL94_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBD4411295.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="KJL94_003027"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="KJL94_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBD4411296.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KJL94_003028"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KJL94_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBD4411297.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="KJL94_003029"
     CDS             13186..14385
                     /locus_tag="KJL94_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBD4411298.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3700 a         3781 c         3910 g         3463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catt
//
LOCUS       DAEBXD010000089        14705 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEBXD010000089 DAEBXD010000000
VERSION     DAEBXD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14705
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.089"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(247..1188)
                     /gene="fabY"
                     /locus_tag="KJL94_003030"
                     /gene_synonym="yiiD"
     CDS             complement(247..1188)
                     /gene="fabY"
                     /locus_tag="KJL94_003030"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBD4411299.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1233..1670)
                     /gene="dtd"
                     /locus_tag="KJL94_003031"
     CDS             complement(1233..1670)
                     /gene="dtd"
                     /locus_tag="KJL94_003031"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBD4411300.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1667..2539)
                     /locus_tag="KJL94_003032"
     CDS             complement(1667..2539)
                     /locus_tag="KJL94_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBD4411301.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2533..3132)
                     /gene="yihX"
                     /locus_tag="KJL94_003033"
     CDS             complement(2533..3132)
                     /gene="yihX"
                     /locus_tag="KJL94_003033"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBD4411302.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3231..4016)
                     /locus_tag="KJL94_003034"
     CDS             complement(3231..4016)
                     /locus_tag="KJL94_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4411303.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4050..4946)
                     /gene="yihV"
                     /locus_tag="KJL94_003035"
     CDS             complement(4050..4946)
                     /gene="yihV"
                     /locus_tag="KJL94_003035"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBD4411304.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5114..6010
                     /gene="yihU"
                     /locus_tag="KJL94_003036"
     CDS             5114..6010
                     /gene="yihU"
                     /locus_tag="KJL94_003036"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBD4411305.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6034..6912
                     /gene="yihT"
                     /locus_tag="KJL94_003037"
     CDS             6034..6912
                     /gene="yihT"
                     /locus_tag="KJL94_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBD4411306.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6929..8170
                     /gene="yihS"
                     /locus_tag="KJL94_003038"
     CDS             6929..8170
                     /gene="yihS"
                     /locus_tag="KJL94_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBD4411307.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8308..9210
                     /locus_tag="KJL94_003039"
                     /pseudo
     CDS             8308..9210
                     /locus_tag="KJL94_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9409..11445
                     /gene="yihQ"
                     /locus_tag="KJL94_003040"
     CDS             9409..11445
                     /gene="yihQ"
                     /locus_tag="KJL94_003040"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBD4411308.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11491..12865
                     /locus_tag="KJL94_003041"
                     /pseudo
     CDS             11491..12865
                     /locus_tag="KJL94_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12908..14311
                     /locus_tag="KJL94_003042"
     CDS             12908..14311
                     /locus_tag="KJL94_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411309.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14379..>14705
                     /gene="ompL"
                     /locus_tag="KJL94_003043"
     CDS             14379..>14705
                     /gene="ompL"
                     /locus_tag="KJL94_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBD4411310.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3580 a         3814 c         3691 g         3620 t
ORIGIN      
        1 ggttttgact atcaaaaatc gctcaaacct gaactaatat gatgagttaa gcattatctg
       61 cattaattgc cactcagctc tacaaataat atttaaatgc tcctgaacag tgatgtagca
      121 agacacgtat ggtgccaacg aagaataaaa atttataaag ttcttattca atataaccgg
      181 aatagtcagg gactgatgta aatgaccgga atgaatgaga gaggcagcac cgcccctctc
      241 cataagctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct tcgcgggcag
      301 aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga tttcgacctg
      361 catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc
      421 aaggtcggct accgcatggg gtttaccgct aatcggtttg ctgtagcgga tatgcgcatc
      481 cgccagaata atcgttccgc cgaggtggcg ttcacgcagc atcagccaga taagccccca
      541 accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt gcggattctg
      601 attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt gaatacgcac
      661 gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct gcgcgcacca
      721 gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca aaaaatggcg
      781 aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca gcttggcgaa
      841 aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc
      901 caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca cgtccgggtg
      961 aacgaccata aagcgaatgg acgcttcatt gtcggcatta atatacaatc ggcctaccgc
     1021 caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc
     1081 gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc gaaactgata
     1141 gtaacgctct aactcttctt ctgtttgtgg aacctgaagg tgatacatag cgatactctc
     1201 tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa tgtcacgggg
     1261 ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg cgtgttcatc
     1321 tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg atccggtgat
     1381 gcgcctttgg agaaacttgg gcgcatcccc cgttcggtat ctgcggcgag ggtaaactgg
     1441 gaaaccacca gtacactgcc gcccgcctgt tgcacgttga gattcatctt gccttcggca
     1501 tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg
     1561 tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc cgtcacttct
     1621 ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat ggttcgtcgt
     1681 cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg acagtaattt
     1741 ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga atggggatca
     1801 ccgccagcac accgtaaatg agctgatatg acgggaacat ggtgatataa agcgcgaaac
     1861 ctttctttcc tgcttcgaac aggagtgcgg cgacaaacgc gccgacaatc gcgtcgcggt
     1921 taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc cacgacaaca
     1981 gcaacggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc gcccagcgca
     2041 gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc cccagcgtta
     2101 aaatcatcca gtacacggcg aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga
     2161 tggtattcaa cgcgctatcg atggagtaca tcaacaataa cgcggtgacg atcagcccgc
     2221 acgccccaac ggcggtcatc ttgttggaat tagcaacaaa ttgttcgata taccgctgaa
     2281 taacatcgcc agtagcaggc agaaagttgg caaaaataaa gtgacgtaac tgaatgctga
     2341 cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta
     2401 atgagagcaa cgacacatag gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa
     2461 tacgttgcca gagtagtttt agccaggccc atagtggacg ggtacggtgc ctggctttgt
     2521 cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg ttttatcttt
     2581 caccagaata ctggtaatgc ctagctgatt agctccttct atattatcgg cgttatcgtc
     2641 gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg
     2701 tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag cagcatcacg
     2761 aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat tggaaagcac
     2821 caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt ccgggcgcag
     2881 cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg gtagagccat
     2941 ctcatgacac agcgcctctg cgaacgcttc atcgctaatt tccccacgct catgctgatg
     3001 aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac gcgttaaatc
     3061 gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac ctaaatcaaa
     3121 gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc gggaaaggat
     3181 gggtttggct atgtggcaag gtatgaagcc aggttaacgg tcacaaaaat ctacgcgtct
     3241 tcctggggaa gaatgatttc aatttggctg tcttgtaagc gtgattttag cgcagcgggc
     3301 ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt taccaatttc aaccgccgcc
     3361 gaagcatgat acttagtgtg atcggcgacc agcagaatat ttctcgcgtg cgccatcatc
     3421 gttttcacca cgttagcttc gtttacatca aactccatca acgcgccatc gctctcaatc
     3481 gccccaacgc ttgttaccag ataatcagca cgaaaatcag ccacaaagga cgccgctgaa
     3541 gggccaatga tcccgctatt atgagagcgc aacgtaccgc cgggcaccat cacttcaaag
     3601 cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt tggtgattat ccgcaaatga
     3661 ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg taccaatggt gataaatatt
     3721 gttgaaccat caggaatata gtctgccacc gcttcggcaa tcgctttttt tttctcggtt
     3781 tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc tggaagcccg acccgcgcca
     3841 ccgtgatggc gcgtaatcag gccttgctcg cttaatttac gaatatcccg gcggaccgtc
     3901 tgcgtggaga catccagcag atttgccagc tcatcaatat tcatataccc acgctcggcg
     3961 atcagcatga ggagttggtc gtgccgcggg ttaccggtta gttcggtaag gctcatgaaa
     4021 attcctcgaa aaaccatcac cctggcattt tatacaaaaa gtgacaaaaa agatcgggtt
     4081 tgatcacagt cagggagccc ggctcgtcca ccgggacgtg tgcattttaa cgccgctaca
     4141 ccgctggcga agcggactga ctccgctaaa tccccacttg ttgccagcgc caccgccaaa
     4201 gcgccgtgaa aaacatcacc cgcacctgtg gtatctacca catcaacttt gaaggccggt
     4261 tgatgctgac gcccaccatt ttccagccag tcgcagcctg cgctaccctg ggtcacatag
     4321 acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac tggccatctc tttcacgccc
     4381 gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat cgcttaatgc caccagctca
     4441 ctgatatcct gcggcgtaat gtccccgtcc agaacggtca tcacacccgc ctgacgggcc
     4501 agggtgaagg cttttttagc gccgtcgtgc cagcgtacat ctgccagcac aacatcccac
     4561 tgagagaaat caatttcctc caaccactct gcgtcaggca gcagatccgg gctggggtag
     4621 ttaatgatta tccgctcgcc tttggtatcc accatgatgg cggattgcga agatttcgcc
     4681 tggttatacc gtttggtgta acgggtgtta acgccccagg attccagctc tgccagcagg
     4741 ctgttgcccg cgtcgtcatc acctacgcga ccaataaaat cgacctgcgc ccccagcctt
     4801 gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa cttccgtata atttctcgcc
     4861 acgtatttac cgctctccgt cggtaaccct tccacgtaat agatgcgatc catcacggtt
     4921 atacctacac aagcaacacg aatcatggtc attccttaaa cattttggga tgcgttcatt
     4981 ttaatttcac caattgttta aaaagtgacg gctgtcaatt ttttgacgat aaattacaaa
     5041 ttcgatcaaa aacagacaag caaagcatgt cataaatgac aaaaagtgac atcactgtat
     5101 tcaggagagg gttatggcag caatcgcgtt tatcggttta ggacaaatgg gttcgccaat
     5161 ggcgagcaat ttattgcagc aagggcatca acttcgcgtc tttgatgtga acgccgaagc
     5221 tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc aacccggcgc aggccgctaa
     5281 agatgccgaa tttatcatta ccatgttgcc gaatggcgat ctggtgcgca acgtgttgtt
     5341 cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg ctggtcattg atatgtccac
     5401 catccatccg ctgcaaaccg ataaattgat tgccgatatg caagccaaag gcttcagcat
     5461 gatggatgtt ccggtaggcc gtacttctgc aaatgccatt accggtactc tgttactgct
     5521 ggctggcggc accgctgaac aagttgaacg tgccacaccg atcctgatgg cgatgggcag
     5581 tgagttgatc aacgctggcg gtccgggcat ggggatccgc gttaagctca tcaacaacta
     5641 catgagcatc gcgctcaatg cgctttcggc agaagccgcc gttttgtgcg aagccctgaa
     5701 tcttcccttc gatgttgccg tcaaagtgat gagcggtacc gccgccggta aaggtcactt
     5761 caccacttcc tggccaaaca aagtcctcag cggagatctt tctcccgcct tcatgatcga
     5821 tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc aaccagctgc atgtgccaat
     5881 gccgctgggg gccgcctcac gggaggttta tagccaggcg cgcgcagcgg gtcacggtcg
     5941 ccaggactgg tccgccattc tggaacaggt ccgtgtcagt gccgggatga ctgccaaagt
     6001 aaaaatgtaa cgactggata aaggaataaa tgaatgaata agtacaccat caacgacatt
     6061 acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc agcgcgaagc catgcgcatg
     6121 atgtttgccg cggctggggc acccgctccg gtagccgata gcgttttaac tgatttcaaa
     6181 gttaacgctg caaaggccct ctcgccttat gcctcggcga ttctggtaga tcaacaattc
     6241 tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa gttgcgccat gattgtcgcc
     6301 gccgatgagt tcattcctgg caacggtatt ccggtcgata gcgtggttat tgaccgcaaa
     6361 atcaatccgc tacagatcaa acaagacggt ggcaaagcct taaaactgct ggtgctgtgg
     6421 cgtagtgatg aagatgcgca gcaacgtctg gatatggtga aagagttcaa cgaactgtgc
     6481 cactcacacg gtctggtaag catcattgag ccagtcgtcc gcccaccgcg tcgtggcgat
     6541 aaattcgatc gcgaacaagc gatcatcgat gccgccaaag agctgggcga cagtggcgct
     6601 gacctctaca aagttgaaat gccgctttat ggcaaaggtc cgcaacaaga gcttctctgt
     6661 gcttcacaac gtctgaatga ccatatcaat atgccatggg tgatcctctc ttccagtgtc
     6721 gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga cggcgggcgc atcgggattc
     6781 ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac cagacaacga gctgatgctc
     6841 cgtgacgttt gcgcaccgaa attacaacaa cttggcgata tcgtcgacga aatgatggct
     6901 aaacgccgct aagatgagga taaaaggaat gaaatggttt aacaccctaa gccacaaccg
     6961 ttggctggaa caggaaaccg accgcatctt tgattttggt aaaaattccg tagtgccgac
     7021 tggttttggc tggttaggca ataaagggca aatcaaagaa gagatgggca cccatctgtg
     7081 gatcacagct cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc gacctggcgc
     7141 ttacgcgttg gttgatcacg gtattaaagc catgaacggc gcactgcgtg ataaaaaata
     7201 tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca aacagggcta
     7261 tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc acaacgggtc acccggaagc
     7321 gcgcaagctg ctcgattaca ccattgagat tatcgagaaa tatttctgga gcgaagaaga
     7381 gcagatgtgc ctggagtcct gggacgaagc cttcagcaaa accgaagagt accgcggcgg
     7441 caatgccaat atgcacgcgg tggaagcttt cttgattgtt tatgacgtca ctcacgacaa
     7501 aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg tcgccagaaa
     7561 taatcattat cgcgttaacg aacacttcga tacccagtgg aatccgctgc cggattacaa
     7621 caaagataac ccggcgcatc gcttccgcgc gttcggaggt acaccaggcc actggatcga
     7681 atggggccgt ttaatgctgc acatccatgc tgccctggaa gcccgttgcg aacaaccacc
     7741 agcatggctg ctagaagatg ccaaaggtct gtttaacgcc accgtgcgcg atgcctgggc
     7801 acccgatgga gcggacggga ttgtttatac cgttgactgg gaaggaaaac cggtggtccg
     7861 cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg gcctacgcgc tctacaccgt
     7921 cactggcgat cgccagtatg aaacctggta tcaaacatgg tgggagtact gcattaagta
     7981 cctgatggac tatgaaaatg gttcctggtg gcaggagctg gatgcggaca ataaggtcat
     8041 caccaaagtc tgggacggca aacaggatat ttatcacctg ctgcattgcc tggtgatccc
     8101 gcgtatcccg ttagcgcccg gcatggctcc agcggttgca gcgggtctgc tggatattaa
     8161 tgcgaaataa ctgatctgaa aatagggtgc gggttttccc ctcaccctaa ccctctcccc
     8221 aaaggggcga ggggaccgtc cggtgcagtt tttgggtttg acatcagcct cacgggaggc
     8281 tgtatgtcgt taattaaggt gccttgtatg caaatcacca atatgcattg cagtggacag
     8341 accgtttcac ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg tgctggtctg
     8401 gctgaattaa cctttcaggg ttgtcattta gtcatcccgc acaaacccga agagatgccg
     8461 ctggcgcatc tgggtaaagt gcttattccg tggccgaacc ggatcgccaa cggctgttac
     8521 cgttatcagg ggcaggaata tcagttaccc attaatgaac atagttcaaa agccgctatt
     8581 cacgggctgc ttgcctggcg agactggcaa attagtgaat taaccaccac cagcgttacc
     8641 ctgacagctt ttctgccgcc cagttacggc tatccgttta tgctggcttc acaagttgtt
     8701 tattcgctca acgcacacac gggtctttcc gttgagatcg caagccagaa tatcggcact
     8761 gtcgcagcgc cttatggcgt gggtattcat ccttatttaa cctgcaactt aacatctgtt
     8821 gatgaatatc tgttccagct tccggcaaac taggtttatg ccgtcgatga acacgtaaat
     8881 ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc gaaaaaaata
     8941 gccgcgacaa aaatcgacca cacctttaaa accgctaatg atttgtggga aatgaccatt
     9001 acccatccac agcaggcgct ttcggtaagc ctttgttccg atcaaccctg ggtgcaggtt
     9061 tatagcggtg aaaaattaca gcgtcaggga ctggcagttg aacctatgag ttgcccgcct
     9121 aacgccttta attcaggtat cgacttatta ttactggaac caggaaaacc gcaccgcctg
     9181 ttttttaata tttacggaca gagaaaataa cttgtattga ttagcagtca aaatcattga
     9241 agaacatttc actcgctaaa ggcaacatta tgtatcttca atttattaag ttaaaaacag
     9301 ctcagcttct gtaaagtttt tatatatcag ttttatgaga atcattttac ataagcctgt
     9361 aataacagga aggcaacaat ctcgttctca actctatgga gattagttat ggatacgcca
     9421 cgtccacagt tattagattt tcaatttcat cagaataacg acagttttac cctacatttt
     9481 caacaacgtc ttattttaac ccataacaaa gataatcctt gtttatggat tggctcaggt
     9541 atagcggata tcgatatgtt ccgcggtaat ttcagcatta aagataaact acaggagaaa
     9601 attgcgctta ccgacgccat cgtcagccag tcaccggatg gttggttaat tcatttcagc
     9661 cgtggttctg acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg
     9721 ctggaactac aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct tgccgctcaa
     9781 ccagaggacc atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgcggcaaa
     9841 ccgttcccgc tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc
     9901 tggcaggccg actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag
     9961 cctacgtttg tcagcacgca gaagtattac tgccatgttg ataacagttg ctacatgaac
    10021 ttcgacttta gcgccccgga ataccatgaa ctggcgctgt gggaagacaa agctacgcta
    10081 cgttttgaat gtgctgacac atacatctcc ctgctggaaa aattaactgc cctgctggga
    10141 cgccagccag aactgcccga ctggatttat gacggggtaa cgctcggcat tcagggcggg
    10201 acggaagtgt gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggttaacggc
    10261 atctgggcgc aggactggtc tgggatccgt ataacctctt ttggcaagcg cgtgatgtgg
    10321 aactggaagt ggaacagcga aaactacccg caactggatt cacgcattaa gcagtggtat
    10381 caggagggcg tgcagttcct ggcctatatc aacccgtatg ttgccagcga taaagatctc
    10441 tgcgaagaag cggcacaaca cggctatcta gcaaaagatg cctctggcgg tgactatctg
    10501 gtggagtttg gcgagtttta cggcggcgtt gtcgatctca ctaatccaga agcctacgcc
    10561 tggttcaagg aagtgatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct
    10621 gacttcggcg agtatctgcc caccgacacg tacctgcata acggcgtcag tgccgaaatt
    10681 atgcataacg cctggcctgc gctgtgggcg aagtgtaact acgaagccct tgaagaaacg
    10741 ggcaagctcg gcgagatcct cttctttatg cgcgccggtt ctaccggtag ccagaaatac
    10801 tccaccatga tgtgggcggg cgaccagaac gtggactgga gtctcgacga tggcctggcg
    10861 tccgtagtcc cggcggcgct gtcgctggca atgaccggac atggtctgca tcacagcgac
    10921 attggcggtt acaccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg
    10981 tgcgatttca gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac
    11041 aactggcagt ttgacggcga tgcggaaact atcgcccatt ttgcccgtat gactaccgtt
    11101 ttcaccaccc tgaaacctta tctgaaagag gccgtcgcgc tgaatgcgaa gtccggtctg
    11161 ccggtcatgc gcccgctgtt cctgcattac gaagacgatg cacataccta ttccctgaaa
    11221 tatcagtacc tgttaggccg cgacattctg gtcgctccgg tacacgaaga aggtcgcagc
    11281 gactggacgc tctatctgcc ggaggataac tgggtacatg cctggacggg tgaagcgttc
    11341 cggggcgggg aagttaccgt taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc
    11401 gatagcgaat gggcggcact gttcgcgtcg ttaaacagca tctaatcaca tccgcccgtg
    11461 gggaaactca tgggcaaagg gagaacatcg atgagtcaca tcacaacgga agatccagca
    11521 actttacgcc tgccctttaa agagaaactc tcttacggta ttggcgacct ggcctctaac
    11581 atcctgctgg atatcggtac gctttatctt ttgaagtttt ataccgacgt tctggggctg
    11641 ccaggcacct atggcggcat tatctttttg atttcaaaat tctttactgc gtttaccgat
    11701 atgggtaccg gcattatgtt ggattcccga cgcaagatcg gtccaaaagg taagttccgt
    11761 ccttttattc tgtatgcgtc attcccggtc accttactgg cgatcgccaa ctttgtcggc
    11821 acaccgtttg atgtcaccgg taaaacggtg atggccacta ttctgtttat gctttacgga
    11881 ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg tcccggcaat caccaaaaac
    11941 cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct gggcctgctg
    12001 ctgtgcacgg tgggattcgt gccagttatg aatcttatcg aaggtaatca gaaacttggc
    12061 tatatcttcg ccgccacgct gttttcactg ttcggcctgc tgtttatgtg gatctgctac
    12121 tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca atccggcgca aaagcctggc
    12181 ctgttgcaat ctttccgcgc aattgccggt aaccgcccac tgttcattct gtgtattgcc
    12241 aacctctgca ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta ttacacccag
    12301 tacgtactta acgatcccat cctgttgtcg tatatgggat ttttcagcat gggctgtatt
    12361 ttcatcggcg tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa gaaggtctat
    12421 atcggcggcc tgctgatttg ggtgctgggc gatctgctca actatttctt cggcggcggc
    12481 tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgcc tgaactgggc
    12541 gctggtttcc gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt cggaaggcac
    12601 ggtctacacc ggcttcacct tctttcgcaa agtgtctcag gcgctggctg gtttcttccc
    12661 cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg accacaccat
    12721 tgaagggtta cgccagttga tcttcatcta cccaagcgca ctggcggtag tcaccattgt
    12781 ggcgatgggt tgcttctaca gcctgaacga gaagatgtat gtccgcattg tggaagagat
    12841 agaagcccgt aaacgcacgg cgtaataata ataaacgacg ccctgcgggg cgttataagg
    12901 agtgattatg tctgaccata atccactgac attaaaactg aatctgcggg aaaaaatcgc
    12961 ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc atcggtactc tgtatctcct
    13021 caaactttat accgatgaat tagggatgcc tgcttactat ggcgggatta tctttctggt
    13081 cgcgaagttt tttaccgcgt ttaccgatat gctcaccgga tttttactcg actcgcggaa
    13141 aaatattggg cctaaaggca aatttcgtcc ctttatttta tatgcagcag ttccggcggc
    13201 gttaattgca acgctccagt ttatcgccac caccttttgt ttgccggtta aaacgacgat
    13261 tgccaccacg ctgtttatga tgtttggact ttcatacagt ctgatgaact gctcgtatgg
    13321 tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg
    13381 ccagggtggt gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc
    13441 gcttttttct gactcaaccg tcggttatgc ctgtgcggca cttatgttct ccattggcgg
    13501 ctttattttt atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcgcc
    13561 aaccggacat aaagccagta ttctcaaatc tttttgcgcg atatttcgta atccaccatt
    13621 gctggtttta tgcattgcta acctgtgtac tctggcggca tttaatatca aactggcgat
    13681 tcaggtctat tacacccagt atgtgctgaa tgatattaat ttattgtcgt ggatgggatt
    13741 cttcagtatg ggatgcatcc tcgtcggcgt attactggtg ccagtaactg taaaatgttt
    13801 tggtaaaaaa caggtttatt tagctggcat ggtgctatgg gcggtgggtg atatactgaa
    13861 ttatttctgg ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg
    13921 cacggccttt gtaaacagcc tgaactgggc actggtacca gataccgtag attacggtga
    13981 atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa
    14041 aatttctgcc gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa ttggttatgt
    14101 tcccaacatc gcgcaaagcg atgcgacatt acagggtttg cgtcagctca tttttatctg
    14161 gccttgtgca ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga
    14221 aaaacggttt gcattgatta ttgaggaaat caaccaacgc aaaaataaag agatggcgac
    14281 agaaaaaaaa acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt
    14341 gggtggcggc ctccctacgt ttaaaaaatg gacttattat gaaaaagatt aatgcaataa
    14401 ttttattatc ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc gaaaacaggg
    14461 aggcatataa tcttgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg
    14521 atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa
    14581 aacatggcta taatgaaatt gaaggttggt atcctttatt caaaccaacc gataaattaa
    14641 ccatccagcc cggtggctta attaatgata agagtatcag ttcgggtggt gcagtgtatc
    14701 tggac
//
LOCUS       DAEBXD010000090        14635 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEBXD010000090 DAEBXD010000000
VERSION     DAEBXD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.090"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="KJL94_003044"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="KJL94_003044"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBD4411311.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KJL94_003045"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KJL94_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBD4411312.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="KJL94_003046"
     CDS             2337..2552
                     /locus_tag="KJL94_003046"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411313.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="KJL94_003047"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="KJL94_003047"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBD4411314.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="KJL94_003048"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="KJL94_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBD4411315.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="KJL94_003049"
     CDS             5918..6415
                     /locus_tag="KJL94_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBD4411316.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="KJL94_003050"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="KJL94_003050"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBD4411317.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="KJL94_003051"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="KJL94_003051"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBD4411318.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="KJL94_003052"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="KJL94_003052"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBD4411319.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="KJL94_003053"
     CDS             9100..9663
                     /locus_tag="KJL94_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBD4411320.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="KJL94_003054"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="KJL94_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBD4411321.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="KJL94_003055"
     CDS             complement(10255..11235)
                     /locus_tag="KJL94_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBD4411322.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="KJL94_003056"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="KJL94_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBD4411323.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="KJL94_003057"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="KJL94_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBD4411324.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="KJL94_003058"
     CDS             12538..12828
                     /locus_tag="KJL94_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBD4411325.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="KJL94_003059"
     CDS             12836..13429
                     /locus_tag="KJL94_003059"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBD4411326.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="KJL94_003060"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="KJL94_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBD4411327.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3529 a         3872 c         3757 g         3477 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttctt
//
LOCUS       DAEBXD010000091        14566 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEBXD010000091 DAEBXD010000000
VERSION     DAEBXD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.091"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..581
                     /locus_tag="KJL94_003061"
     CDS             <1..581
                     /locus_tag="KJL94_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBD4411328.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="KJL94_003062"
     CDS             complement(635..1357)
                     /locus_tag="KJL94_003062"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD4411329.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="KJL94_003063"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="KJL94_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KJL94_003064"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KJL94_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBD4411330.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KJL94_003065"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KJL94_003065"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBD4411331.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KJL94_003066"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KJL94_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBD4411332.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KJL94_003067"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KJL94_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBD4411333.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KJL94_003068"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KJL94_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBD4411334.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KJL94_003069"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KJL94_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBD4411335.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KJL94_003070"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KJL94_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBD4411336.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KJL94_003071"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KJL94_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBD4411337.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KJL94_003072"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KJL94_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBD4411338.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14566)
                     /locus_tag="KJL94_003073"
     CDS             complement(13620..>14566)
                     /locus_tag="KJL94_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4411339.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3718 c         3317 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atcccg
//
LOCUS       DAEBXD010000092        14479 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEBXD010000092 DAEBXD010000000
VERSION     DAEBXD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.092"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(113..379)
                     /locus_tag="KJL94_003074"
     CDS             complement(113..379)
                     /locus_tag="KJL94_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBD4411340.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="KJL94_003075"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="KJL94_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBD4411341.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="KJL94_003076"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="KJL94_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="KJL94_003077"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="KJL94_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBD4411342.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="KJL94_003078"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="KJL94_003078"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBD4411343.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KJL94_003079"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KJL94_003079"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBD4411344.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KJL94_003080"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KJL94_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBD4411345.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="KJL94_003081"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="KJL94_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KJL94_003082"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KJL94_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBD4411346.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="KJL94_003083"
     CDS             complement(12641..14095)
                     /locus_tag="KJL94_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBD4411347.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="KJL94_003084"
     CDS             complement(14092..>14479)
                     /locus_tag="KJL94_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4411348.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3652 c         3420 g         3546 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAEBXD010000093        13963 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEBXD010000093 DAEBXD010000000
VERSION     DAEBXD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.093"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KJL94_003085"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KJL94_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD4411349.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KJL94_003086"
     CDS             2219..2767
                     /locus_tag="KJL94_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBD4411350.1"
                     /translation="MGKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KJL94_003087"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KJL94_003087"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBD4411351.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KJL94_003088"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KJL94_003088"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBD4411352.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KJL94_003089"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KJL94_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBD4411353.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KJL94_003090"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KJL94_003090"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBD4411354.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KJL94_003091"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KJL94_003091"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBD4411355.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="KJL94_003092"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="KJL94_003092"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBD4411356.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KJL94_003093"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KJL94_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD4411357.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KJL94_003094"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KJL94_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBD4411358.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3562 c         3526 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 gggcaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAEBXD010000094        13956 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEBXD010000094 DAEBXD010000000
VERSION     DAEBXD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.094"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="KJL94_003095"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="KJL94_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBD4411359.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="KJL94_003096"
     CDS             complement(1604..1777)
                     /locus_tag="KJL94_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBD4411360.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="KJL94_003097"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="KJL94_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBD4411361.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="KJL94_003098"
     CDS             3415..3744
                     /locus_tag="KJL94_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBD4411362.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="KJL94_003099"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="KJL94_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBD4411363.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="KJL94_003100"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="KJL94_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBD4411364.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="KJL94_003101"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="KJL94_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBD4411365.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="KJL94_003102"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="KJL94_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBD4411366.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="KJL94_003103"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="KJL94_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBD4411367.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="KJL94_003104"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="KJL94_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBD4411368.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="KJL94_003105"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="KJL94_003105"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBD4411369.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3454 c         3244 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccaag tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttaccccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttttcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaaacagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcaga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAEBXD010000095        13947 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEBXD010000095 DAEBXD010000000
VERSION     DAEBXD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13947
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.095"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(216..2483)
                     /gene="adiA"
                     /locus_tag="KJL94_003106"
     CDS             complement(216..2483)
                     /gene="adiA"
                     /locus_tag="KJL94_003106"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBD4411370.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2682..3590)
                     /gene="melR"
                     /locus_tag="KJL94_003107"
     CDS             complement(2682..3590)
                     /gene="melR"
                     /locus_tag="KJL94_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBD4411371.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3873..5216
                     /gene="melA"
                     /locus_tag="KJL94_003108"
     CDS             3873..5216
                     /gene="melA"
                     /locus_tag="KJL94_003108"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBD4411372.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            5331..6752
                     /gene="melB"
                     /locus_tag="KJL94_003109"
     CDS             5331..6752
                     /gene="melB"
                     /locus_tag="KJL94_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBD4411373.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6891..7520)
                     /locus_tag="KJL94_003110"
     CDS             complement(6891..7520)
                     /locus_tag="KJL94_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBD4411374.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALFGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7643..9289)
                     /gene="fumB"
                     /locus_tag="KJL94_003111"
     CDS             complement(7643..9289)
                     /gene="fumB"
                     /locus_tag="KJL94_003111"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD4411375.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQIAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9367..10707)
                     /gene="dcuB"
                     /locus_tag="KJL94_003112"
     CDS             complement(9367..10707)
                     /gene="dcuB"
                     /locus_tag="KJL94_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBD4411376.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11278..11997)
                     /gene="dcuR"
                     /locus_tag="KJL94_003113"
     CDS             complement(11278..11997)
                     /gene="dcuR"
                     /locus_tag="KJL94_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBD4411377.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11994..13625)
                     /gene="dcuS"
                     /locus_tag="KJL94_003114"
     CDS             complement(11994..13625)
                     /gene="dcuS"
                     /locus_tag="KJL94_003114"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBD4411378.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13806..>13947
                     /gene="yjdI"
                     /locus_tag="KJL94_003115"
     CDS             13806..>13947
                     /gene="yjdI"
                     /locus_tag="KJL94_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBD4411379.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         3320 a         3370 c         3607 g         3650 t
ORIGIN      
        1 gttttactaa tattacaaat ttttatagta atttctcatg tacatatttt tttacaggat
       61 aaataacagg caaaaaaaag cgcggggtgg ggccgcgcaa aaagtatgac atttttgttt
      121 gcagcaattt ttatgaaaac gttgccatac tcaaaaggcc taaggccagg cggcgtaatg
      181 ttatttaaac aattacgcct tcagcggaat agtggttacg ctttcacgca cataacgtgg
      241 taaataccgt caataatttc agtcccttca gtttcgtgtt caaatccagg gaaatggtgg
      301 tcccaggatt gcagcgagcg taaataactt acttgcggac tgtttttatc gccgaagttt
      361 tcaccagaca gcagcatcgg gattcctggc ggatacggga taactgagtt tgccgcgatg
      421 cgtcctggca gattttcaat ggataccagt tcgacattgt tgtcgacaat cgcgttgtac
      481 gcttcacgcg gggtgatttc cgccaccggc aggccggaat aggcttcgtt caaccgtgcg
      541 ccagggttgt tttctttcag ccaggcaaac atggtgtcac ccagatcgtg aatccccatg
      601 ttcgcgtaag tgtcaggata ttgttcaaca agttccggca tcacctgcgc cagcggtgtg
      661 ttggcgtcat agtggtgttt gaaggagcaa agggtgttaa ccagagtccc ccatttccca
      721 cgggttacgc ccatagagaa caggaacata atttggaagt cagtggtgcg ggtaggcaca
      781 atgccgtggc gaccaagcca ggcagtgacc agcgccgccg gaacaccggt ttcttccagt
      841 tcaccatctt cacccattcc cggagcaagg atgctgactt taattgggtc gagcatactc
      901 cagttatccg gaatatcttt gaagccgtgc cagctttcgc ccggatgcat tacccagcag
      961 tcctgaacgg tggtcagcag tttggttggt gcgtcagcaa agtcataggt tttgccggtt
     1021 tgtgggtcgg tgacgacttc tttgttccac ggtttgaaga accagctacc gtcagcggtg
     1081 aactctttat atagccgcgc catcgcctga cggaaatcaa ccgcttcgtc aatcacttcc
     1141 tgtgtcagtg acaggccgct gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg
     1201 cagatggcat acagcgggga ggtggtggca tgcatcatgt aggcctggtt gaagcgggag
     1261 aagttaatcg ccccacgacc ttcacgtaca tgaatataag aagcctgtga cagcgcattc
     1321 agcagtttgt ggatggagtg ggtggcgaaa acggtaggac cgttgtgatc gccaggttcg
     1381 ccgcgcatgg catagtgatc ggcatagatc gggttgaaac gtgcatagcc gtaccaggct
     1441 tcgtcaaagt gcagacgatc ggaggttttt tccagcagat cctgcgcttc tttagcgtta
     1501 taacacacgc cgtcataggt gcagttggtt accacgcagt aagacggttt ttgcccggct
     1561 ttgtctttgg tcagcgggct ttcactgatt ttcttttgca aggtttcagg ttgcatttcc
     1621 tgcggataga ttggcccgat aatgccgtag cggttgcggc ttggcaccat atagaccggt
     1681 ttcgcgcctg tcagcatcaa accttgttcg atggatttat ggcagttacg gtcaacgacc
     1741 acgacatcgt tatcggtcat gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg
     1801 acgactaccg accaggagcg atcggcacca aatacgcgtg cggcatattt ttcgctttcg
     1861 ccaaatgcgc cagtatggtc aagcaaagaa ccgagggaag ttcgttcgat gcccatgtcg
     1921 gtgcggaaca gattttcacc atagtagtca tggtagaaac gtccggcggg tgttttggta
     1981 aaaccaacgc cgccctggtg gcctggcgct gcccaggaat attcatggat gtcactatat
     2041 ttcatcagcg cgctgaacag tggcggcaac agctgctggc ggtagcgggt catcgcggca
     2101 acggcgcgtc cggcgataaa gtcggcggta tcttccagaa tccaggcgaa ttcatcgaca
     2161 agctccagca gatcccggtc cattgcggcg agggcttttt cccgatcgcc caacaggaag
     2221 accggcacgt tttgttggcg ctcatgaagc ttaccgatca attgtctgac gttttgatgt
     2281 tcgtccggat gctccatttg atagctgaac atcaggcagt caatggcttc gtttgaagag
     2341 agaatggcaa aaccatcatc aaaggaggtg gatttaatca cggtaacatt ttgctggctt
     2401 aaagcatctg ccagacgctc aacggcgtta ccgacccagg tgtcttgatg gagaaactcg
     2461 ctttcaacaa ttaatacttt catcattgct tacccggtta tgaaggaatc ttcgttgcgg
     2521 gcaagtatct tccggccttt tcgggctgta aagcgcgtga aaaacaaaca tttttgaaat
     2581 atttagtttt ataaacaata cgttatgtga ttattttaaa ttattttcac tgcttattat
     2641 cgacggctaa actatttttt tggctgatac tgatatcgtc tttagccggg aaacgtctgg
     2701 cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca tgccgacata tttgccgaac
     2761 gtgctgtaaa aacgactact tgaacgaaag cctgccgtca gggcaatatc gagaatactt
     2821 ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc gcatcgcggt aatgtactgt
     2881 ttcatcgtca attgcatgac ccgctggaat atccccattg catagttggc gttaagtttg
     2941 acgtgctcag ccacatcgtt gatggtcagc gcctgatcat agttttcggc aataaagccc
     3001 agcatctggc taacataaaa ttgcgcatgg cgcgagacgc tgtttttgtg tgtgcgcgag
     3061 gttttattga ccagaatcgg ttcccagcta gagaggctaa atcgcttgag catcaggcca
     3121 atttcatcaa tggcgagctg gcgaatttgc tcattcggac tgtttaattc ctgctgccag
     3181 cggcgtactt caaacgggct aagttgctgt gtggcaagtg atttgatcac catgccgtga
     3241 gtgacgtggt taatcaggtc tttatccagc ggccaggaga gaaacagatg catcggcaga
     3301 ttaaaaatcg ccatgctctg acaggttccg gtatctgtta gttggtgcgg tgtacaggcc
     3361 cagaacagcg tgatatgacc ctgattgata ttcacttttt cattgttgat caggtattcc
     3421 acatcgccat cgaaaggcac attcacttcg acctgaccat gccagtggct ggtgggcatg
     3481 atatgcggtg cgcgaaactc aatctccatc cgctggtatt ccgaatacag cgacagcggg
     3541 ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat ctgtattcat ggatggctct
     3601 ctttcctaga atatcagaat tatggcagga gtgagggagg atgactgcga gtgggagcac
     3661 ggttttcacc ctcttcccag aggggcgagg ggactgtccg agcatcatga ggccgaaaac
     3721 tctacttttc aggtaattta ttcccataaa ctcagattta ctgctgcttc acgcaggatc
     3781 tgagtttatg ggaatgctca acctggaagc cggaggtttt ctgcagattc gcctgccatg
     3841 atgaagttat tcaagcaagc caggagatct gcatgatgtc tgcacccaaa attacattta
     3901 tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc catcgcgagg
     3961 cgctgaaaac ggcgcatatt gccctgatgg acattgaccc cacccgcctg gaagagtcgc
     4021 atattgtggt gcgtaagctg atggattcag caggggccag cggcaaaatc acctgccaca
     4081 cccaacagaa agaagcctta gaggatgccg attttgtcgt ggtggcattt cagattggcg
     4141 gttatgaacc ttgcacggtg actgatttcg aggtctgtaa gcggcatggt ctggaacaaa
     4201 ccattgccga tacgttgggg ccgggcggta ttatgcgcgc gctacgtacc attccgcatc
     4261 tgtggcaaat ttgcgagaac atgacggaag tctgccccga tgccaccatg ctcaactatg
     4321 ttaacccaat ggcgatgaat acctgggcga tgtatgcccg ctatccgtat atcaaacagg
     4381 tcgggctgtg ccattcggtg cagggaacgg cggaagagct ggcgcgtgac ctcaatatcg
     4441 acccggctac gctgcgttac cgttgcgcag gtatcaacca tatggcgttt tacctggagc
     4501 tggagcgcaa aaccgccgac ggcagttacg tgaatctcta cccggaactg ctggcggctt
     4561 atgaagcagg gcaggcaccg aagccaaata ttcatggtaa tactcgctgc cagaatattg
     4621 tgcgctacga aatgttcaaa aagctgggct acttcgtcac ggaatcgtca gaacattttg
     4681 ccgaatatac gccgtggttt attaagctag gtcgtgagga tttgattgag cgttataaag
     4741 taccgctgga tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg cataaagagc
     4801 tggaggagta taaaaacgcc tcccggattg atattaaacc gtcacgggaa tatgccagca
     4861 caatcatgaa cgctatctgg actggcgagc cgagtgtgat ttacggcaac gtccgtaacg
     4921 atggtttgat tgataacctg ccacaaggat gttgcgtgga agtagcctgt ctggttgatg
     4981 ctaatggcat tcagccgacc aaagtcggta cgctaccttc gcatctggcc gccctgatgc
     5041 aaaccaacat caacgtacag acgctgctga ccgaagctat tcttacggaa aatcgcgacc
     5101 gtgtttacca cgccgcgatg atggacccgc atactgccgc cgtgctgggc attgacgaaa
     5161 tatatgctct tgttgacgac ctgattgccg cccacggcga ctggctgcca agctagttgc
     5221 accgttaaaa cgcgactaaa cgctactgcg ccgggggatt tattccggcg cacacctctg
     5281 acgataccaa taacagaagg cgggcgttgg taacagcgac ccgataccct atgagcattt
     5341 caatgactac aaaactcagt tatggatttg gagcgttcgg gaaggatttt gcgatcggca
     5401 ttgtgtatat gtacctcatg tattactaca ccgatgtcgt cgggctgtct gtgggtttgg
     5461 tcggtacttt gtttctggtg gcgaggatct gggatgctat taacgatccg attatgggat
     5521 ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa accctggatc ctgatcggta
     5581 cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc gcatctgttt gaaggtacta
     5641 ctcagattgt ctttgtttgc gtgacctaca tcctctgggg catgacttac accattatgg
     5701 atattccctt ctggtcgctg gttccaacca tcacgctcga taaacgtgaa cgcgaacaac
     5761 tggttcctta tccgcgtttt tttgccagtc tggcaggctt tgttacggca ggtgtgacgc
     5821 taccatttgt taattatgtc ggcggtggcg atcggggatt tggctttcag atgttcactc
     5881 tggtattgat cgcctttttt attgtttcaa ccatcatcac tctgcgcaat gtgcatgaag
     5941 tcttttcgtc agacaatcaa tcgtctgctg aaggaagcca tctgacactt aaagccatcg
     6001 ttgcgcttat ttataaaaac gatcagcttt catgcctctt gggtatggct cttgcttata
     6061 atgtagccag caacattatt accggatttg ctatctatta tttctcatat gttatcggtg
     6121 atgcggattt gttcccctat tatctgtcgt atgcgggagc tgctaacctg gtgacgttag
     6181 tattcttccc acgcttagtt aaatcattat cccgacgcat tttatgggcc ggagcatcta
     6241 ttcttccggt gttaagctgt ggtgttctcc tgttaatggc attaatgagc tatcacaacg
     6301 tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt ttctgggtat
     6361 tacaggtcat catggtggca gataccgttg attacggtga atataaactg cacgtacgct
     6421 gtgaaagcat cgcttactcc gtgcagacta tggtggtgaa gggcggttca gcctttgcgg
     6481 cttttttcat tgcggttgtg ttagggatga ttggctatgt accgaatgtt gaacagtcta
     6541 cgcaagccct attaggtatg cagtttatta tgattgctct accgactctg tttttcatgg
     6601 taacgctgat tctctacttc cgtttctatc gcctcaatgg cgacacgctg cgcaggatcc
     6661 agattcatct gctggataaa tatcgcaaag taccgcccga gcctgttcat gctgatattc
     6721 cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca atagctaacg aaaatggcct
     6781 gatgcgatat gtttatcagg ccacgtctta atgtaatgct ttggatttgc aaattttgta
     6841 gaccggataa ggaattcacg ccgcatccgg catcaacaaa gtgcacgttg ttatccagtt
     6901 atcaagccat agcgccgtag ctggcggcaa tgaaagcgag cgagcaatat cactgtcgtt
     6961 aatgcgccaa acagcgcgca gaacatatcg gattgcgtgt cccactggtc gccctgggta
     7021 ccgagaaaat catccgctcc ctggcccatc gccagtgccg cccaccactc gattaattca
     7081 tacatcgcgc ttatcgccag cgccacgcaa cagaccagaa acgccaccat tttacgtccg
     7141 cgcacgtaca tcccgcgaac gagaatttct cgtgccacca gtgcaggcac cagcccctgg
     7201 aaaaagtgcc ccagcttgtc atacggatta cgactcaacc ccagccattc ctgcacctca
     7261 aaaccaacgg ggactttcgc gtaggtgtac tgcccgccta ccatcaggat gatggcgtga
     7321 aagaagatga gcgtatagag cagcagcgtt aacgggtaac gtctggcggt ggcaagtagt
     7381 agcggcacga cgataatcac cggtgtcact tccagcagcc aggtgagttt gtcatgggcc
     7441 gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccggtgtt aagaattaac
     7501 ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga ctattcacca
     7561 gcaagcatga aattacaatc gcaaaagagg tggcccaggg ggatcacctg gcagcatgct
     7621 gtcaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg ttgacgattt
     7681 gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa tcttctactt
     7741 cgattttcca gatagcttcc atacccagct ccggataagc gacgcattcc agatgcttga
     7801 tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag aagccgccgt
     7861 gtttatgaca cgcgtcggta acctgctggc tgcggttgcc tttcgccagc atgatcatgc
     7921 tgccgccgtg ggattgcagc agatccacat aggagtccat acgacctgcg gtggttgggc
     7981 caagtgaacc tgatggataa ccggcagggg ttttcgccgg acccgcgtag tagatcgggt
     8041 gatctttgat gtactgcgga agttctttac cggcgtcaat cagctctttc agcttggcgt
     8101 gtgcaatatc tcgacccaca ataatggtgc cagtgagcga caaacgagtg gaaaccggat
     8161 attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcgacc ttcaccgctt
     8221 cgccttcacc ggcctgacgc agttcttgtg gaatgtactg acctagattg tgttccagtt
     8281 tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca gcggagcagg
     8341 agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata acgcgaatgt
     8401 cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg gcctcttcca
     8461 gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc ccttccgtcg
     8521 gcagttcatc gtaatagtga gcgcttgcta atttgacggt tttcaggttg gtttccgcag
     8581 acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta ccgagggtac
     8641 gcattttctc gacgaggaag tttttcagtt tgccgggagt cagcagggct ttggtttcct
     8701 ggtagagata cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg aatttgtact
     8761 catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttagtgccg gtgttgacct
     8821 ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag gttatcttcg atataggtgt
     8881 tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg cgctggcctt
     8941 ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg cctttggcgg
     9001 cgatttcgga gtttcttaag aattgcagtg ccacgtactt gtcgttttcg ctggcttctg
     9061 gatcgtgaag aatagccgca atctgtttct ggtgtgccgg acgtagcatg aaagaagcgt
     9121 cgtgaaaggc ttgctgcgcc agcagggtca gtgcttctgg ttccactttc aggatggttt
     9181 cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat tcggtattgt
     9241 ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata gcttccagcc
     9301 tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc ggcacgcaaa
     9361 gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc acgatacgct
     9421 cacaccaatc aaccccggca gaataaaact gtggttgatg acgaagcgac cgatgtgggt
     9481 ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg gcaggatgta
     9541 ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc cgatcgccag
     9601 cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact tggaaaccag
     9661 cagcagaaca atggcatacg cccacggata ctctttcacc atttcaccca gtacgccctg
     9721 aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac cgtacaccgc
     9781 cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg cgggattggt
     9841 tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa taaccagtac
     9901 catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac caagaagggc
     9961 gactacagcg attgccccga ggaaaatcca cattgccagc cagttgcttt tcggcagttt
    10021 tttatccagc agcgtcgcgg tatcaccgta aacatactca cggttttccg gtacggagat
    10081 gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc tgaagatacc
    10141 gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcagat cgaggaactc
    10201 aagatggcga ccatcaaagg tgacattacc cagcatcgca accagagaca cgaccgcaac
    10261 cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg ccatcggacg
    10321 ttccggacgg atgttgttct taatggcgac gtcgtagatg atcggcagaa tggtgtaaac
    10381 cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc acaaacggcg cgacaattga
    10441 gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga catcaagacc
    10501 gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca gcatgacatc
    10561 aactggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca gaccgatacc
    10621 gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac atattagtat
    10681 tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct atttaaattt
    10741 ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga gcctgactaa
    10801 atatccgttt ggtcgttacc ggctttagca aatacctcac agtgaatatt ggctgattaa
    10861 agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaaa actctctggc
    10921 gatgggttta tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt atcttattga
    10981 tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttata gattaatctg
    11041 atctacccat ttgtgggtaa aaatacacat aatgcgggtg acataatagt taattaactt
    11101 ttgttatcgt tttgaaatta aaaacaccgt tcatctgaag agatattaat ttttaacgat
    11161 gatggaggga taattatatt tgatctggca caagttttac tgattaacga tgtaacttgt
    11221 gccaggggta ttttgcatta cggtaataat tattacttac agataaccag cgatccgtta
    11281 ttggcaatat tgtttcagca gtgagtagtg ttctgcctga atacggtaac ggtaaaccgg
    11341 acgccccgtg acgccataat ggatactggt gaacaagatg tggcagttga ccagccagat
    11401 gaggtatttt cggcaggaaa cacgcgagat gttaacctcg ttagccagct cgtcggttga
    11461 aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg tctgcggcgt
    11521 taagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt ggattagctg
    11581 atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct tttgccgcca
    11641 gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt aatccacgac
    11701 accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga tgacaatcac
    11761 atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt tctctttttg
    11821 catatagata tcgagcaata tcaggtcgat aggcgcatcg ctattgaaga taatctcttt
    11881 ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt gtgctacgta
    11941 tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat tgatcatctg
    12001 ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat ccccggttcc
    12061 gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag tgctaaaccg
    12121 acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc gattttgtcg
    12181 ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg acggtagtgc
    12241 aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc gttttctatc
    12301 agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct gtctggcagc
    12361 tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt aatcttgctg
    12421 attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc aatctcttcc
    12481 tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact cttcagatgc
    12541 aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc acgaagtgcg
    12601 tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc agttttgtcc
    12661 ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac ggtgttgatc
    12721 agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc gtcgcgtaac
    12781 acttccgaga catctaccac ctgtgaccat gagtggctga gcgtcgacag tttctcatcg
    12841 tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag cgtgacctcg
    12901 ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc ctggcgttgc
    12961 tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt cagtacctta
    13021 accagaatgc aggtgccaat cagtccgacc agcatgccaa ataataccga ccagataatg
    13081 ctccagcgac tgtcattgat ctgttgggtc acgcggctta actcaaggcc gatcgccacc
    13141 acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa agcctgcgcc
    13201 agaaaaccgc gattgatagc gacattttct tcgccattca gcgcattaag gatgtcatca
    13261 cctttaaatg gctgaccaat acgctgggct tcaggatgtg agtagcgaag actttgcata
    13321 tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc gatggcctgg
    13381 atgccactct cctgcggttt tttctgcaag ccctgacgga tttccggcga gtcggcgagg
    13441 gtacgcgcca ctgccagtgc cttgttggcc agcccgtctc gcgtcatatc actgatttgc
    13501 gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct gaccattaag
    13561 atcacagtgg tactcaattt catcggacgt ttgcgtaata tgtggtaggg caatgaatgt
    13621 ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag gcgggtaatt
    13681 caaagggagt aaaaatgatt ggctatatag gggaagagac tctggcaacg gaaactgcca
    13741 gtgctgtatg aagattccgg ggctatgctt atagcgataa tcatactgat gagagaggga
    13801 aggtcatgga tcaggcgcta ctggacgggg gttatcgctg ttataccggc gaaaagatcg
    13861 atgtctattt caacactgcg atatgtcagc attctggcaa ttgcgtacgt ggcaacggca
    13921 agttatttaa tctcaaacga aagtcgt
//
LOCUS       DAEBXD010000096        13931 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEBXD010000096 DAEBXD010000000
VERSION     DAEBXD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13931
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.096"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            409..1239
                     /locus_tag="KJL94_003116"
     CDS             409..1239
                     /locus_tag="KJL94_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD4411380.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KJL94_003117"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KJL94_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBD4411381.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="KJL94_003118"
     CDS             complement(2368..3828)
                     /locus_tag="KJL94_003118"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBD4411382.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KJL94_003119"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KJL94_003119"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBD4411383.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="KJL94_003120"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="KJL94_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBD4411384.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KJL94_003121"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KJL94_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBD4411385.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KJL94_003122"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KJL94_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBD4411386.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KJL94_003123"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KJL94_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBD4411387.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="KJL94_003124"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="KJL94_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBD4411388.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="KJL94_003125"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="KJL94_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="KJL94_003126"
     CDS             complement(12470..13009)
                     /locus_tag="KJL94_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4411389.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="KJL94_003127"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="KJL94_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD4411390.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3436 a         3544 c         3315 g         3636 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaactgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt g
//
LOCUS       DAEBXD010000097        13800 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEBXD010000097 DAEBXD010000000
VERSION     DAEBXD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.097"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="KJL94_003128"
     CDS             complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="KJL94_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBD4411391.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGT"
     gene            complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="KJL94_003129"
     CDS             complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="KJL94_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBD4411392.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="KJL94_003130"
     CDS             complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="KJL94_003130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBD4411393.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="KJL94_003131"
     CDS             complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="KJL94_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBD4411394.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="KJL94_003132"
     CDS             complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="KJL94_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBD4411395.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="KJL94_003133"
     CDS             complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="KJL94_003133"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBD4411396.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6044..7183)
                     /gene="wza"
                     /locus_tag="KJL94_003134"
     CDS             complement(6044..7183)
                     /gene="wza"
                     /locus_tag="KJL94_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBD4411397.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7492..7641
                     /locus_tag="KJL94_003135"
     CDS             7492..7641
                     /locus_tag="KJL94_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411398.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7842..9425
                     /locus_tag="KJL94_003136"
     CDS             7842..9425
                     /locus_tag="KJL94_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD4411399.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="KJL94_003137"
     CDS             complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="KJL94_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBD4411400.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="KJL94_003138"
     CDS             complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="KJL94_003138"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBD4411401.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12425..13066)
                     /gene="udk"
                     /locus_tag="KJL94_003139"
     CDS             complement(12425..13066)
                     /gene="udk"
                     /locus_tag="KJL94_003139"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBD4411402.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13384..>13800
                     /locus_tag="KJL94_003140"
     CDS             13384..>13800
                     /locus_tag="KJL94_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HBD4411403.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3640 c         3495 g         3472 t
ORIGIN      
        1 gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac accaacccgg
       61 aatacagcca gagaaatcaa tgctaacggt aattttttct taatcgcctc tttattcaga
      121 tattgaaatg cccactccag caaataaaac aggataaagg tcataacccc tgaaaacgat
      181 ccggataata ttatccctgc gagaatcagg gcatctgttt taggcgtttt gataccaaac
      241 tgtttgatgc tgagccaaat tgagattaat gccagagcga aaaatgccgg ttcgaaataa
      301 agtgctgtcg tgcgcttgcc gccgaattta atgaaattca gcacatagct gttgctgtaa
      361 atcagatatt tcgaaatcga ctccataata ctgctgccac cggtgaggat aatttgcgcc
      421 atctccaccg ccgccagcgc caccaccagt ccgaccacca gataaaagaa acgtaatatc
      481 ttacgatggt tgtgtggcga aattgtttta aaacgaatac tccacaccat gccaataatg
      541 atcacaatat agacaaacag catagttgaa gtgacgtatt taccggcatc caacgactga
      601 ccaaacaggt agttaaacgc cgtgagtccc gcgccaatcc ctaaggcaat catcaatttt
      661 ttaatgctga tgcgttctaa aaacagcagt aacaagacag gtaaaaaagt gacgatggtg
      721 atggggaaac tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta gatcagtggc
      781 agcagcaggt agctacagat tctgatagaa gttgacatac tcctccagca tctgttgtcc
      841 actgtaggcg gcgcgactgc gttggctgaa ctcagccagc gtggtaccaa atatcgcctg
      901 cgcgatttcc ggtttgctta actgtaccag ttgcagcacc tcttcttcac tgacggtttt
      961 accaccggat ttttgcagca cttcccgcgc cgcatcgcta tgggtggcaa tcaccggcac
     1021 gccaatcgat agcgcctcac acaaaatcag cgggtagtta tcgacacgag aactgaacac
     1081 cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt caaagccgtg
     1141 attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca gttcgatttt
     1201 gtcgcccagc gccatcatct cacgcaccag ttgctggtta gttttgccgt cgtaacgcag
     1261 gtcatgcgcc accactgcga ttttcggctt gccctgagtt tcgcgcaccg gaggcagatc
     1321 tgccagaatc gcttcggttg ccatatcaat gccattattg ataatccggc aacgccctgg
     1381 cccgtacaga ctattgaaag cgtcagccac atgctggctg ggggaaataa actgacagcc
     1441 cagcgccagt atctcgcgga ataactggcg tttgcccgcc accagctggt gcgcgcgatc
     1501 aatcttcacc ggcggatagt tatttaaggt cgggcatttc tggcagcccg ttttccagcc
     1561 ttcgcaaccg tcggtaaagg cgcagcgtcc ggttacactc cagtggtcgt gcagcgtcca
     1621 gaccagggtg acatccggtt tatggttttt cactttttcg caaaagcgca ccacgctgtt
     1681 aagattcaac cagtagctgt gcagcacatg aaaatgcagg accaccggac ccggtgtgcg
     1741 agtaatggtg cgatataact cattgaaatt gccgaacaga tcgcgattaa acagacgaaa
     1801 caacgcaatg ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct gcggatagtt
     1861 ctgatggctg acgctctctt tgccgccttt gccgtagccg tagacaaaat gtgacgccag
     1921 cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc cgccttccgc
     1981 cagtcgcaca ttaaattgca gaatattcat ttaatcacct tcactcgcgc tttttctccc
     2041 accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc cacggtgacg
     2101 ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc gacgccgttg
     2161 ccaatgtgtg gacatgccat gttatcggca ccccgattgc cgatagtgac gccgtggcga
     2221 atggtgaaat catcccccgc caccacgttt ttattgatca ctacggcgta accgtgatgg
     2281 atagtaaagc ggcggccaat ggtcgcggcg gcctggattt cataaccgaa aaagcattcg
     2341 gtgataatgc gatacagcac cagcagcggg gccgcccaca gattgttgag gacgttcttt
     2401 ttgcgccaca ccgaacaaaa atgagcgacg cgataggcaa gtaccatgca gcacgggcgt
     2461 aaactccagc tgttggcgcg cagatcttcc agcatcttta gcgcccccga ataccatcag
     2521 ccagacgttt gccgttacgc accgacagca gcgtcaacaa ggtgcgccag gtcatacgtt
     2581 tattgcggat ctggtagagg gtaaacagct gatatttttt actggcgcgg tcgaatttgt
     2641 ctttgtgctt gcggtaaaag tggaagtatc cggagaattt tttcggtgac gaggtgatct
     2701 gcatttcgcc gtgattgata tgcaggatct gcgtcgcctc ttccactttc cacggttcgc
     2761 cgtactccac caccatccgc aggaatatgt cgtaatcctg tgctgctttc agttcggtat
     2821 cgaacaaaca ctctttgaaa cgccatgccc aggtaaagac ctgattgcca atgatattac
     2881 gtttatagaa caggcggcgt gagtacggcg atttgggata cagtggtaag ctcgccggtt
     2941 gggaataaac ttctccctgg caaacgtagt cgttagcgta caaaaaggcg tgtgtgacca
     3001 gttgctgttt atgggcgagg aagacgctca ggcggttggg cgtccattca tcatcatcat
     3061 cgatcccggt gatgtattcc ccttgcgcca gcataatcgc ctggttacgc accgcgcacg
     3121 ccccgctgtt aatgtcgttg tggatgtaag tgatacgcgg atcgttgagg gcagtgacgt
     3181 actgttgcag ttgttcccag gaggtggagc aatcatccac gatgatcatc tcccagttgc
     3241 tgtagtcctg gcgcaggacc gattttatcg cccgaatcgc cagttgttgg cggttccagg
     3301 ttggcatata gattgagatc agcggattgt ctttcatagg ttgttctccc ggcttatgag
     3361 ctggtttgct tgccggatgc ggcgtgagcg ccttatccgg cctacggggc ggtgcgaatg
     3421 caggcgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat aatcctgata
     3481 cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa taccgttttg
     3541 ctcaaagcgg ctcagactgg tttccacttc tttcaatgtg ttgaccgcat aacgcgccac
     3601 cattaacgtg gttccgacat gacgaccaac aattgccgca tcggtcactg ccagaatcgg
     3661 cggcgtatca atcaacacca ggtcatagtt tttactcgcc cagttcacca gttcggcaaa
     3721 gcgttcgctc attaacagtt cagaaggatt tggcggcacc tgaccgcgcg ggatcaggtc
     3781 aaatttggca atagaggtcg gtttagcagc ggtagtaata tcgccctgac caatcagaat
     3841 ctccgacagc ccattgacat tattggtgcc caacagctcg tgggtgtagc ctttgcgcat
     3901 atcgcagtcg atcaacaaca cgcgtttatt ggtctggctg atcaccgccg ccaggttggc
     3961 gcagacaaag gttttaccga ttgacgggct aaccccggtc atcatcaaca cattgttctg
     4021 cgcctgcatc atcgcgaagt gcagactggt acgcagacta cggatggctt caatcgccag
     4081 atcggttgga ttccccactg ccagtagctg gctctgttta tagcgtttaa cccctttgat
     4141 ggttttgacg ctatcgcgcg ctttctgcca ctccgacagc gggatgctgg catagacgct
     4201 gattccgtgt tcttccagca cttgcgggct ttcgatgccg cgattaaaca acgagcgcag
     4261 cagcacaccc acgatagaga gcatcaggcc aaggataatc gcgccgagga taatcagccc
     4321 tttcttcggt tttagcacgc caggctgagt gattgccggg tcaataatgc gcacatcgcc
     4381 gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt gcatatagac
     4441 ctgctgacca gactcgacat cgcgggtcag acggacaatt tcctgctggt ttttcggcat
     4501 cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac gtttctccag
     4561 cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg cctctttaaa
     4621 ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg ctttcgcttc
     4681 aagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt tttcggcaac
     4741 atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc ttttcgacgc
     4801 ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgagcgatgc tgttaagaat
     4861 gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc ctgcgtcttt
     4921 gccgttctcc gttaccgtca ggctgttttg cagttggttg atcatcccca acgtggagta
     4981 tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca tcagcgtgac
     5041 gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc catcactgct
     5101 cagggtgtag tttttgtcat ccagcacatt aagcgtaaac acctgatccg ccatctcttt
     5161 cgggcggtta aaggtagtca ctttcaccgt ctcgttctga cgccccatca ggcgatccca
     5221 gcccgccccg aaaatcggga acgtgttttt gctcaccgca atatcgaggt cgagatcgtc
     5281 caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt ccgatgcagg
     5341 cggtttgttg gctaatgccg agccgatatc ctgcaccagc gaattgccgc tgttttgctc
     5401 gatttgtacc agtgcgtcgg cactataaat cggcgtggcg aagaaagtgt aaaccacggc
     5461 acagagggca aatacgacgg tgatgccaat cacccaccag cgcgcttcaa tgacggtgcc
     5521 gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat gttgttttac
     5581 tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg gcgggcagac
     5641 cgttcaagta atgtgtacac cgctgcaaac gtttcccggc ttttgcgata cggatcgggg
     5701 atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat ctccggtgcc
     5761 atctcgcata agcgttcgat atggcgctta tccatggtca aaatcaggtc atagttgcga
     5821 cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg atgttctgcg
     5881 gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag tccagcggac
     5941 tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccatcgg ggaacggcaa
     6001 atattgccga cacagacaac taagatgttg ttaaacatga cgattaccag ttatgaatgt
     6061 cgctggctgt atccgtcatg taacggacac cgctaatagt tggcagcaac tgattgatca
     6121 gacggttcca gcgggaaacc ggggcggtgg tgacatacac cacgtcataa ggttgcaggc
     6181 ggaattctgt cgccatcacc agcgacgtcg catcggacat atccagctgg tagatattgg
     6241 caatcttgcc gttacgcccg ccctcgcctt tcagcggacg aatgacaaag atgccgctgg
     6301 cgttggaggt ggtcatgtcg ataccttcgg cattggccag agcttcagtc agggtcatgc
     6361 cgctaaagtc cattttcagg gtgctctgtt tcttcacttc acccatcaca aatactttca
     6421 gatcatcatt acgcggcacg tagagaatat cgccgggata aagcagtcgg ttctgattga
     6481 ggtcgccgtt ttgcatcagc gcctgtaaag aaatgcgttc ttcacgacca ttgtgtgtta
     6541 gcaccacgtt gcgccagtca gcggtgtcgg tcaggccacc tgcggcgttg atggcgtcga
     6601 gaatggtcag tggtacgttg gtgatcgcct gttgaccgga tttattcacc tgacctgaga
     6661 tataggcctt ttgtgagcgg aaggcggcga tattaacgtc cacctgcggg tcagcgatgt
     6721 acgtcgctaa gcgcccggta atatcactgc ggatttcagc gagcgttttc ccgactacgt
     6781 gaaccttgcc gatatacggg taaaacatag tgccgtcagg ctgtacccag ttgccggtgt
     6841 cgctggagct gcggtactga ccagctggcg tggtgagttc cgggtgatcc cagacggtga
     6901 cattaagaac gtcccccggc ccgacgcgat actggtaatt cgcgatctcg ctttccagcg
     6961 tcatattggg gcgcgctaca ttcgggcgtg ggcgtaattg gtcaatcagg cgcggggtca
     7021 gcggataaac attcaccatt ttgtcgagat cgaaatcagc gtcctgctgt ttgatgacgt
     7081 ctttgcccat cgtcgacata ttgctgcccg gaagtactgt gcaaccgctt atcaaggtta
     7141 ctgacaccaa taatggcatc aatttcattt tggatttcat cattgtttat ttatcacttt
     7201 ggcagagtaa ttatcctgtg cactattaat agcaatgtcg ccatgcacat ttaccttgca
     7261 gttaattgaa taaaaattta actggcatca gtcctaaaaa aattgatttc atccgcaggc
     7321 tattgacaga ataattcaga ctggtctttc aggcatccag acacgctacc gcccctggct
     7381 ttttagctac caatacactg attgagctta atttttcaaa ccctctctgc atacaatcgt
     7441 tgatgagaaa gggttattgc ggaaattaac ttccgaatat aaggtgacat tatggtaatt
     7501 gaatattggc tcgccaataa tgcaggagga agtgttacag ctaacggaat agcaggcaag
     7561 gtaacaattc ggcaattggc tatttttaag aattatatta agtatcattc aatatggttt
     7621 ttaggagttt ctttaggttg acaatattta atattgcgtc tccacatgcg atatttctta
     7681 aataatgttt tattattacc gcttttaatt cagggataat gtgaggttat taccctcaaa
     7741 taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc catgatcgaa
     7801 ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc
     7861 cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata
     7921 acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac
     7981 gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt actgctagcg tcaatctcct
     8041 ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt agcgcccgcg
     8101 acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg gagctgaacg
     8161 aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg aagttctggg
     8221 gcgtggtgac gcaaattgtg gtactggacg ccattttctc actcgactca gtgattaccg
     8281 ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc
     8341 tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta
     8401 ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct
     8461 tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcac
     8521 tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaatcag acgctacgcc
     8581 agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaagat gcggaactgg
     8641 acgccaaaac cgcgtccatg ctggtggatc atggaaacca gcagatcttt aatccgcagg
     8701 aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtaagc agcattatga
     8761 cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagaagaa atccgccagt
     8821 tgctggagcg aaatcagcat acgcggctgg tggttaccga cggtgatgac gcagaagatt
     8881 tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc actgcgcggc gaaccgctca
     8941 acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg ttgttaccag
     9001 ccctggagca gttccgtaat gcccgcacgc actttgcttt tgtggtggat gagtttggct
     9061 cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga
     9121 acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac ggttcctgga
     9181 cggcgaatgg tcatatgccg ctggaggatc tggtgcaata tgtgccgctg ccgctggatg
     9241 aaaaacgtga atatcacacc attgccgggc tgttgatgga gtatttgcag cgtattccaa
     9301 aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc
     9361 atcgcgtgca gaaggtgcag attataccgc tgcgtaagga tggcgagatg gagtacgagg
     9421 tgtgatggta tcggccattt tacccaatgc tgcgtaacgc cttataactt cagcaatgaa
     9481 gcgttgtctg tggtaaacgg acatcacagc gggggtgtgg gtccggctgt aaatcgccga
     9541 atcgtttcct gtaggctggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
     9601 gatgttacct cgcgcccgta cccgatagcc gaaaggaata agatgaggga accgcgccag
     9661 cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc caccctggca
     9721 cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg aacagccttc
     9781 gatcttacat attcagaata taatagtgcc gccgcgaacg cctgatgcga cgctttacgc
     9841 gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca gcaacttctt
     9901 cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca ggcgacgttt
     9961 cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcaggctgt aattgagttg
    10021 ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat caatcagttt
    10081 gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc aggtttgatc
    10141 tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac cttgcatgtc
    10201 gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt caagccgcgt
    10261 cacgttatcg aagttttcag cggccttcac atcaccgcca ttacgctcta ccgcttgctg
    10321 aatcatctgc tggaagttca tcccttccat acgtgtgtcg gtcatctcaa catgcgcctg
    10381 tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga aatcaccagc
    10441 cagtgacatt tttccggtca acgaaatcgg atagttaaac gccttcagaa tggtaccaat
    10501 ctcaacgttc tccagccgtg gctggaagtt tatccgcgga tttattgatg tcgcgtccag
    10561 cgtgcccggc agtgaaacct ttccaccgtt aagcttgccc tgcagttgag taatttccag
    10621 caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc cgcgccagcg
    10681 cacgttactg gcctgcaaca aaatatcagc tgtaaagcct tgcagtccct gataggccgg
    10741 ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct gcccctgctg
    10801 cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt caagattcag
    10861 ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga cctgtgcttg
    10921 tccgctcagc gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg
    10981 tgactcctgc cactgggact ggaaactccc ctgcccctga ataccttgtt ttggtaaatc
    11041 ggcaccctgc aactgccagt taatttgttc aatagccgct gttaaatcat gcggataatc
    11101 agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc gattaacccg
    11161 cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca ggcggatatt
    11221 gcggattgtc acctgctcgt catcttcatg ctggaacacc agaacgctgt ccgccacctt
    11281 aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca aggtattgtc
    11341 gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg tcagttggat
    11401 cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta gtggtaaaag
    11461 cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc cctgggcggt
    11521 gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac gcagtggccc
    11581 gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa
    11641 atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca cgaccaagag
    11701 tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga attagtcttt
    11761 atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt cacggcggtt
    11821 gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc
    11881 cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa tgcaaccaga
    11941 ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac gcgccaaaga
    12001 ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact ccagtgtcac
    12061 cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga gaacgatctc
    12121 atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc tcagatcgat
    12181 aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta catccaccgt
    12241 cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc cttcatcaag
    12301 ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg cacatcactc
    12361 ccttaacgcc aattacgtca agggcatacc aacttatcac tgaacggtac acaatttatc
    12421 aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg atgcggtttt
    12481 tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg aattgcagga
    12541 acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa cgcccacgct
    12601 cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta tcaacgaaaa
    12661 tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa
    12721 tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt tcaacatagc
    12781 tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc tcaagcaaca
    12841 ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct tccatcgaca
    12901 gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt tcatcaccga
    12961 cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg gaagccgatg
    13021 cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat ttagcgacct
    13081 gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat tatagggatt
    13141 tcatccgtct gataccagtc gaatagcgtt gccgcgcgct cagagttaat tgttgacaaa
    13201 gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat gttatacgca
    13261 gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa
    13321 cttttgtact agcataaaca cagaaacgaa tactggcgac ctggccttgc ggataaagcg
    13381 gtaataagca aacaatcaca gcatgtatta attgccctgc cccacccgct gcttcacctg
    13441 gtcagtttag gtttagtctc gtttatcttt acccttgtct cgcttgagct ttcgcagttt
    13501 ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc gttttatcgc
    13561 catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa tatcgccgca
    13621 tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat caatattgtt
    13681 gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa ccccttgcaa
    13741 aatctggctg actggctgcg tctggcattc ggcagcgcca ttgttccgcc tctgttgggg
//
LOCUS       DAEBXD010000098        13585 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEBXD010000098 DAEBXD010000000
VERSION     DAEBXD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.098"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(337..582)
                     /locus_tag="KJL94_003141"
     CDS             complement(337..582)
                     /locus_tag="KJL94_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411404.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="KJL94_003142"
     tRNA            complement(1227..1302)
                     /locus_tag="KJL94_003142"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="KJL94_003143"
     CDS             complement(1408..2115)
                     /locus_tag="KJL94_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBD4411405.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="KJL94_003144"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="KJL94_003144"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBD4411406.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KJL94_003145"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KJL94_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBD4411407.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KJL94_003146"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KJL94_003146"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBD4411408.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="KJL94_003147"
     CDS             complement(7299..7574)
                     /locus_tag="KJL94_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBD4411409.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KJL94_003148"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KJL94_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBD4411410.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="KJL94_003149"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="KJL94_003149"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBD4411411.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="KJL94_003150"
     CDS             9534..9860
                     /locus_tag="KJL94_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBD4411412.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="KJL94_003151"
     CDS             10044..10763
                     /locus_tag="KJL94_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBD4411413.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="KJL94_003152"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="KJL94_003152"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBD4411414.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="KJL94_003153"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="KJL94_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..>13585)
                     /locus_tag="KJL94_003154"
     CDS             complement(13164..>13585)
                     /locus_tag="KJL94_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBD4411415.1"
                     /translation="SWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDM
                     TPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQ
                     HLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3499 a         3247 c         3518 g         3321 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggaca
//
LOCUS       DAEBXD010000099        13558 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEBXD010000099 DAEBXD010000000
VERSION     DAEBXD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.099"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="KJL94_003155"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="KJL94_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD4411416.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KJL94_003156"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KJL94_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD4411417.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KJL94_003157"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KJL94_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD4411418.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KJL94_003158"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KJL94_003158"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD4411419.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="KJL94_003159"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="KJL94_003159"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD4411420.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /locus_tag="KJL94_003160"
                     /pseudo
     CDS             complement(5235..5849)
                     /locus_tag="KJL94_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="KJL94_003161"
     CDS             complement(5911..6954)
                     /locus_tag="KJL94_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBD4411421.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="KJL94_003162"
     CDS             complement(7200..7652)
                     /locus_tag="KJL94_003162"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4411422.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KJL94_003163"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KJL94_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBD4411423.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KJL94_003164"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KJL94_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBD4411424.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KJL94_003165"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KJL94_003165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBD4411425.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KJL94_003166"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KJL94_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBD4411426.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="KJL94_003167"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="KJL94_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="KJL94_003168"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="KJL94_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBD4411427.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="KJL94_003169"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="KJL94_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         3377 a         3514 c         3395 g         3272 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacggga
//
LOCUS       DAEBXD010000100        13532 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEBXD010000100 DAEBXD010000000
VERSION     DAEBXD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.100"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..890)
                     /locus_tag="KJL94_003170"
     CDS             complement(<1..890)
                     /locus_tag="KJL94_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4411428.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="KJL94_003171"
     CDS             1131..1931
                     /locus_tag="KJL94_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD4411429.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="KJL94_003172"
     CDS             2009..2779
                     /locus_tag="KJL94_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4411430.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KJL94_003173"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KJL94_003173"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBD4411431.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KJL94_003174"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KJL94_003174"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBD4411432.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="KJL94_003175"
     CDS             5046..5768
                     /locus_tag="KJL94_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4411433.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KJL94_003176"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KJL94_003176"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBD4411434.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="KJL94_003177"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="KJL94_003177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBD4411435.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="KJL94_003178"
     tRNA            7161..7237
                     /locus_tag="KJL94_003178"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="KJL94_003179"
     CDS             complement(7368..8414)
                     /locus_tag="KJL94_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBD4411436.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KJL94_003180"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KJL94_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KJL94_003181"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KJL94_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBD4411437.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KJL94_003182"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KJL94_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBD4411438.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="KJL94_003183"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="KJL94_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KJL94_003184"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KJL94_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3522 c         3611 g         3298 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct gaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tg
//
LOCUS       DAEBXD010000101        13109 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEBXD010000101 DAEBXD010000000
VERSION     DAEBXD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.101"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            212..862
                     /gene="csgD"
                     /locus_tag="KJL94_003185"
     CDS             212..862
                     /gene="csgD"
                     /locus_tag="KJL94_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBD4411439.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            867..1256
                     /gene="csgE"
                     /locus_tag="KJL94_003186"
     CDS             867..1256
                     /gene="csgE"
                     /locus_tag="KJL94_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBD4411440.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1281..1697
                     /gene="csgF"
                     /locus_tag="KJL94_003187"
     CDS             1281..1697
                     /gene="csgF"
                     /locus_tag="KJL94_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBD4411441.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1724..2430
                     /locus_tag="KJL94_003188"
                     /pseudo
     CDS             1724..2430
                     /locus_tag="KJL94_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(2563..3054)
                     /locus_tag="KJL94_003189"
     CDS             complement(2563..3054)
                     /locus_tag="KJL94_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBD4411442.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3156..3710)
                     /gene="ycdY"
                     /locus_tag="KJL94_003190"
     CDS             complement(3156..3710)
                     /gene="ycdY"
                     /locus_tag="KJL94_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBD4411443.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3734..4471)
                     /gene="ycdX"
                     /locus_tag="KJL94_003191"
     CDS             complement(3734..4471)
                     /gene="ycdX"
                     /locus_tag="KJL94_003191"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBD4411444.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4526..5464)
                     /gene="ghrA"
                     /locus_tag="KJL94_003192"
                     /pseudo
     CDS             complement(4526..5464)
                     /gene="ghrA"
                     /locus_tag="KJL94_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5699..5786
                     /locus_tag="KJL94_003193"
     tRNA            5699..5786
                     /locus_tag="KJL94_003193"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5733..5735,aa:Ser,seq:gga)
     gene            complement(5936..6217)
                     /locus_tag="KJL94_003194"
                     /pseudo
     CDS             complement(5936..6217)
                     /locus_tag="KJL94_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6499..7503)
                     /locus_tag="KJL94_003195"
     CDS             complement(6499..7503)
                     /locus_tag="KJL94_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HBD4411445.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7575..8135)
                     /locus_tag="KJL94_003196"
                     /pseudo
     CDS             complement(7575..8135)
                     /locus_tag="KJL94_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8220..8417)
                     /locus_tag="KJL94_003197"
     CDS             complement(8220..8417)
                     /locus_tag="KJL94_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411446.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8429..8920)
                     /locus_tag="KJL94_003198"
     CDS             complement(8429..8920)
                     /locus_tag="KJL94_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411447.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8917..9291)
                     /locus_tag="KJL94_003199"
     CDS             complement(8917..9291)
                     /locus_tag="KJL94_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBD4411448.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9338..9712)
                     /locus_tag="KJL94_003200"
     CDS             complement(9338..9712)
                     /locus_tag="KJL94_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBD4411449.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9875..10096)
                     /locus_tag="KJL94_003201"
     CDS             complement(9875..10096)
                     /locus_tag="KJL94_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBD4411450.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10159..10635)
                     /locus_tag="KJL94_003202"
     CDS             complement(10159..10635)
                     /locus_tag="KJL94_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBD4411451.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10651..11124)
                     /locus_tag="KJL94_003203"
     CDS             complement(10651..11124)
                     /locus_tag="KJL94_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4411452.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11466..12287)
                     /locus_tag="KJL94_003204"
     CDS             complement(11466..12287)
                     /locus_tag="KJL94_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBD4411453.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12387..12560)
                     /locus_tag="KJL94_003205"
     CDS             complement(12387..12560)
                     /locus_tag="KJL94_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBD4411454.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(12680..>13109)
                     /locus_tag="KJL94_003206"
     CDS             complement(12680..>13109)
                     /locus_tag="KJL94_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411455.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3313 a         3235 c         3222 g         3339 t
ORIGIN      
        1 aatgagttac attttgttac atgtttaaca cttgatttaa gatttgtaat ggctagattg
       61 aaatcagatg taatccatta gttttatatt ttacccattt agggctgatt tattactaca
      121 cacagcagtg caacatctgt cagtacttct ggtgcttcta ttttagaggc agctgtcagg
      181 tgtgcgatca ataaaaaagt ggggtttcat catgtttaat gaagtccata gtattcatgg
      241 tcatacatta ttgttgatca ctaaaccttc tttgcaggcg acagctctct tgcagcacct
      301 taaacaatcg ctagcaatta caggaaaatt acataatatt caacgttctc tggacgatat
      361 ctcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc ttatccatta
      421 ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc taaatacgcc
      481 tgaagattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg ttttttatgc
      541 catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgtg gcgaatgcta
      601 ctttacgcaa aagcttgcca gttacctgat tacacattct ggtaactatc gttataacag
      661 cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg
      721 cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca
      781 tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg tttcctgggc
      841 aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt gtggcggcag
      901 aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg ggattgttaa
      961 ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt gataaatggg
     1021 aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga tggggaagct
     1081 ggatcacaat aacggtcaat caggatgtta ttttccagac ttttttattc ccgttgaaaa
     1141 gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc
     1201 gccagataaa tcaggcgtta ttaagtacgg acgatttggc gcatgatgaa ttctaaataa
     1261 aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc atgcttattt
     1321 cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac tttggtggta
     1381 acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct tataaagatc
     1441 cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac tttactcagg
     1501 ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt aaaccgggcc
     1561 gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa ttgcagttga
     1621 acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt ttacaaaata
     1681 actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc gcttatttct
     1741 tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag aagccgccag
     1801 accgacatta atgcctcgtg ctcagagcta caaagatttg acccatctgc cagcgccgac
     1861 gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat ttaaacccta
     1921 cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc agggcttaca aaacctgctt
     1981 aacgagcgca aggttattcg tgcggcacaa gaaaacggca cggttgccat taataaccga
     2041 atcccgctgc aatctttaac ggcggcaaat attatggttg aaggttcgat tatcggttat
     2101 gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt ttgggatcgg tgcagacacg
     2161 caataccagc tcgatcagat tgccgtgaac ctgcgtgtcg tcaatgtgag taccggtgag
     2221 atcctctcct cggtgaacac cagtaagacg atactttcct atgaagttca ggccggggtt
     2281 ttccgcttta ttgactacca gcgcttgctg gaaggggaag tgggttacac ctcaaacgaa
     2341 cccgttatgc tgtgtctgat gtcggctatc gaaacagggg tcattttcct gattaatgat
     2401 ggtatcgacc gtggtctgtg ggatttgtaa aataaagcag aacggcagaa tgacattctg
     2461 gtgaaatacc gccatatgtc ggttccaccg gaatcctgac agataaaaat gccggaatta
     2521 ataatccggc atttactgtt taagcgaaag cgtgagtgcc cctcacgctt tatttttgat
     2581 ttcctgctca cggtgagccg tctttgcact acgcgccgcc agccacaggc cgctgttttt
     2641 cattgcgtaa ccaaatacca gcccaagcgc cagcgaaggt aacaccagtt tccagtcacc
     2701 ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag
     2761 ctgtttggcc tgaatacaca tcagaaacgc gacaataccg gttatgacat aaccgagaat
     2821 ttccagatgt ggtgccagtg cactaccgta aataatgacc atcgcccaca ccacgccact
     2881 aagcagggtt gcagcggaga tcgccagccc tttcagccca ccttgcgggc aagcaaagta
     2941 ggccgtacag cccaggaagc ccgcccagct tagtaagccc agggaaacag ccacccatcc
     3001 ccagataccg gagagaatgc ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg
     3061 catgatagca gaaaacgcgg ccatgaatga gatcgagcac acattttatg caacaacaaa
     3121 ttatctgttg cagttatgag tgacgatgat cacatttact cttcagaatc ttcttccagc
     3181 tcgtcccaca ttgcactaat ggcatcacgg gttagcggtg ccatggtgcg ccagaaaggc
     3241 gtggttgcat gggcctccac tttgccgagg aacgcaccac accagggtaa cagatactca
     3301 ctgaacagtg tttccagtgc ttcgctctca tcttccgttg actgatcttc cagccaggaa
     3361 gccgcaagca gcaatgtgcc gatgtgatcg gctggcgtat ccgccagtgg catccctcgt
     3421 tcggaaagaa aagcgcgcac ttccgcttcc gttgcgtcct caacccatgc gctacgatat
     3481 ggcggcacgg cacattcatc gccgataaac aacgcattgt aatcggcaga gacttgcgtc
     3541 atatcacagc ttttctgtaa acgcgtcagt aactcatcct gctccagtgg ccagttcgca
     3601 gccagtttcc cctcacgaat cagagtaaac agcggcacca gtaaaggatc ttgcggttgg
     3661 cggtaataga gcgaacccag cacacgacag aggatagaaa actcgttcat taatatattt
     3721 ccattactag taattaaagg tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa
     3781 ggaagttcag taagcggcgc ggagaaacat tcaaaatgcg ctctagcgga aaatctaccg
     3841 cgtcgaggat tttaagacac tcttcaaatt cccccatggt aaacgcggtg tgagaatccg
     3901 agcctaatgc cacccaacct ccagcatcac gtaccgctgc agctaccgca cgacagttgt
     3961 cttcactgcc cttacgtgag tgtaaaaatg aggaattatt gatttccagc gccacctgat
     4021 gtttcgcggc tgcttcagca acggctttaa catctatttc atatttcgga tttccgggat
     4081 ggcttattat atgcacattg ccgctggcga tagtggagat catcgcttgt gtattggtcg
     4141 ctttgtcatg tggcgcaaaa accggctcat gaaaaccggc aataatcaga tccagcgagt
     4201 caaacatttt accgctgcag tcaatttcac catcaacatt tttaatgtta gcttcaatgc
     4261 cgcgcaggat ccctacccca tcaaccactc gcggccagat acgcatgtta atgaagtgcc
     4321 agtgatgcgg cgcatcttcc atatccgggc catgatcggt gatcgcaaaa agtttaatgc
     4381 ccttttgttt ggcctgggca atgtaatcac tcaatgtgct atatgcatgt gtgctggcaa
     4441 cggtatgcat atgaaggtcg acgggataca taactctctc ctgtattcaa ttcccgccaa
     4501 ggatagcagg aatcctgatg ctttattagt agccacgtgc gcggtcgact tgcccgcaga
     4561 ccctctcccc tttttcgagc tgggcaatag tgcgagaaat gtactccaca gcttcagcgg
     4621 gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac
     4681 tttcaggcgg taagggttca cgattaaaaa catccagcat tgcgccttta actttcccgc
     4741 tatccagcgc cgcgagcagg tcatcttcca caacatgaac accacgcgcc aggttgagga
     4801 gatacgcgcc atcaggtaat ttttcgagta attgttgatt aataatgccg acggtttcag
     4861 gggtattcgg taacaaatta atcaataccc gacattggct cagaaatgca gacagttctt
     4921 cccgtccggc aaagctttgc acgccaggcc acgatttacg ggttcgactc cagcaacgca
     4981 gcggaaagcg ctaggtttgc agactctgag caactttaca gcccagtacg cctgcgccca
     5041 aaatgccgat ggtaaaatct tcccgatgat attcaggcag cggttgccaa tgcgaactat
     5101 tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat
     5161 attcctgcat ttgctcgccc ataccggtat cttccaggcg aaaaagtgga acagaagggt
     5221 tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg gccccgagtg
     5281 cgaacaccgc tttaagatcg cgccctgcca gcatttcaac aggaggatgc cagactaaaa
     5341 cataatcagc agagtcatta tctccgcttt tccatgccct gactcttgcc tgaggaatag
     5401 ctttgcgcag tgcctcaatc caccattggg tatcgaacgt tgggtgataa aagatgatat
     5461 ccatactgac tcccgaaaag cgttgtgcga aatttattcg cacttatcgt tatgatctac
     5521 aaaggccacc agcataacaa atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta
     5581 aaaccacaca ttgattgaat gtttgaataa acgcgcgatt ttttcaaaaa gtttgttgac
     5641 ctcaggtcat gatttcccta aattagcgcc cgttccagca agacaggaac gacaatttgg
     5701 tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg
     5761 gggttcgaat ccccccctca ccgccagatt ataagtgcat gttattgttt aacatgcgct
     5821 tttttatttt tccttccaga cagtatgcca tcgggtatgc catctgaaac cggtttatat
     5881 gccccgcgat catatcttat cggacaattt ccgatctttg agcatactgg caaagctata
     5941 ctgccagcat atcaggatag tgttttgcga taatatcatt cagtctgtca accagttctg
     6001 gttttctgaa cgtgatatgc gcggagccct tcttaaagta tctgatgctg aacatctcat
     6061 cttcatagct ctctttaccc tgaacagact gaatgtgatc atccagacga agggtgatgt
     6121 ttccccggtt gtcggggatt ggtttaccgc tgaacaggtg caacattctt tccagatcgg
     6181 cgagtcggtc agcctgttgt ccggtgataa gatgaaatct atggtcaccc ccgtttttgc
     6241 aacactgatt tttgattaat ggttggcttg cttaaatcta tccggcgtct ctgtgggcaa
     6301 ggatgcccgc gccccgatga gttccgcacc tgataatcct aaaaatcccc tcggcttcag
     6361 tgagccattt catctgttag gtttttaatc aggtcgattt gccgttcatg tcatcaataa
     6421 tcagtcttcg caaaaccaga aaggtaactt gtatcttatt gtttaaaata aactaattga
     6481 gttatatcag catctatatc aggcctgata ttcttttttc tgagtagtga ttgcccaggc
     6541 aattcgagcc agtttatttg ccagagcgca ggtaaccaca ttcgaatgct tcttgttgag
     6601 ttgctcccga acccactcag caagttttcc atgcttgcgt tcaagtcgca ttatgaatga
     6661 gcgggcacac tgaaccagta accggcgtaa atttttatca cctcgcttac tgatacccaa
     6721 cagagtcgat tttcctccag tgctatactg ccggggaaca agtcctgtgg aagctgcaaa
     6781 atccctgctg catgagaact gtttcccgtc accaagttgc gacgatagta agctggcagt
     6841 aatcggacca acaccaggaa ttgtcataat gcgctgagcg gtatcatcag cgttgattga
     6901 ctgacttaat tctgattcca gctctgctat ttgctcaaca aggtaaaagt agtgggtatg
     6961 caacctcacc aaaaggcggc tcagatattc ggggatttcg tgttcagcca acacgagaga
     7021 caaccgtttt attacagcat cacctatcgg caggctgatt ccaaattcca gcaagaaacc
     7081 gtggatctgg ttggttgttt tcaccttatc tctgatgagc gattctctga cccggtgtaa
     7141 cgccctcatg gcctgctggg tctcagttct tggctggaca aaacgcattg atggtcgtga
     7201 agccgcttcg catatggctt ctgcatcaac aaaatcattc ttattacttt tgacaaacgg
     7261 gcgaacgaac tgtggagaaa tcagcttagt ttcatgccca aaatcagaga tacagcgagc
     7321 cataaagtgt gcacccgcac aggcttccat tacaacaact gagggcggac atgtcgccag
     7381 aaactgcatc agtttagtac gggtaaattt tttacgcaga agagccttac cagacttgtc
     7441 ttgacaatga atgtggaagg agtgctttcc gagatcgata ccaataagtg tgatgttctg
     7501 catgatggtc tctccgttaa agggatttac cttaacagcg tactgctgtt aaggtggggg
     7561 tgaccatatc attatcccca ccggtcccat ctcaccagat tgttgacgat aattttactg
     7621 ccaaatttac aggggcaatt ggttttgtaa ttccagttca gccctctgaa gacgttgatc
     7681 acccctcgct caaacacctc atccttattc tggtgcagtt gttcaaatgt gctcagaatg
     7741 ttcgcttcgc tgatttccgg aaaattatcg tactccagtg accggtacca tgtatcacgg
     7801 gcctgcgtat ccattaatga cagcatcccg gaacgttgca tcaggtcacg ccagatttcg
     7861 cgatcaagat tccgggtaat ggctttcatt gctgtttcgg gtttctccat cagccagcag
     7921 ccgcagcgaa aatcctgttt catggcccag tccggggcag ttttacctcc gatgctttcc
     7981 gtcagcaccg agatttcagc cagttgtcgg ataagtgtgt cgatttgcaa cagtgtactc
     8041 tttcgtacag ccagaatgtg gcgaatgctg gttgagcaga taacgtccgt atgcccgttg
     8101 accacttccg gacgggagat tgtgatatct gacataatcg atattccttt ttgcagataa
     8161 atgaaaacgc cagccctgtg caggctggcg tcagaggaat gaagtctggt gagtgagctt
     8221 caccggttct ggtgcagaag ctgcagatga cgcagggtac ggacatcctc ccgggcctgt
     8281 tcgatacagg gcaacagtgc tgctgaatcc gttttatcct catcgttgtc aaagataatt
     8341 cccgatccgc agtcgatatt gtcctgcagc cacgcaatca gaatatccag cgctgtttcc
     8401 gtggttaatg atttcatgtt gttcctggtt accagtcgaa agtgggtaaa cccggcaggc
     8461 attcagcgct ggcatccaga tgaatgagac tgacgccata acgccggatg agtgtgacga
     8521 ccagacgacg gaacgttttt gacagcccca ggcgtttaag ccgcagaacc gggtatgacc
     8581 acgcatcggt acgtaacaga tatccgctac ctgtaaaatg aatccattct gattcaccga
     8641 agtcactggt ctggtgtgac agcgagtaca accaggcgtt gtccgtttcc gtgatatgtg
     8701 cggtactgca gcgtatgccg gtaagggtag caaatggtgg cgtgcattct gctgaggtct
     8761 ggtcagattc atcaacaaca cgaagggagt agccgttatc gcagaccatg ttaatcagtt
     8821 cagcgagctc aataccatcg acgtcaacga caatgcgccc tatgttcagt gcctgtacgt
     8881 taacgctgtc ggcttccagt gtcagggcca gtttcatcat ttcgcctccg gatacttacc
     8941 cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg tcgcccggcg
     9001 agcccggagg atatcaatac tgtttattaa ctgagagcgg ggacaggtgc tgaatcccgg
     9061 ctggtcggta cgcaccagcg cgtatttttc cacgagaaaa ttaactgcat cacacagtga
     9121 aatgcctgcc tcaatatgct gctcaatcac acgttcatcg acaaacggtg tgtcattcag
     9181 tgtgaggcca taatgctggt ccagcagtcg gatgagcagt gtctgccaga tggtgacggg
     9241 agacgggcag cgagatacct cccgtacatg tgtgtcggat aatgttttca tcttaagttc
     9301 tctgtaactg gtagtgctaa aggtgagagc ttcggggtta tgaggtggtt gcgggtgctg
     9361 gtgtcggata aacagccaga taaacgtaac cacaggagcc gagggtgtcg gcttcgcagg
     9421 tcagcccttt tgcatacagc gtgacggtat gctgatatcg gggattcagt tcaccgctgg
     9481 tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccaga tggtatgcat
     9541 ctgcgtcgct gaagcggcct ctgataccgg cgcggtcggc aaggtaatac aggcggttac
     9601 cctcttgcac cagacgagcg ccaaaacagg gcctcactgt gcagggaagc ccccaccagg
     9661 ggcggtcgtg attgtcatcg ggatgagtta tcccggagag tttgtctgac acgataaaat
     9721 cctctctgaa aagtggttaa gaatggttta acgatgacag gtcagccggc gcgctgaccg
     9781 gtttcggtga agatgtatgc attgatgaga atggcctcat ccacagcggc gtctgaggtc
     9841 atctggtcgt actcgtttcc cgggtgacgg aacatcagtt cagggtgaca ctcatcagcc
     9901 ggacatacgg gccaaaactg tccttacggc gttctgcaaa cacggcaaga acgccgggaa
     9961 tatcctgcac ttcacgacca gtatacgctt cagcactgcc gtgccagcga tacttaccgg
    10021 tacagaacgg aaaaagacgg gatgcaggat gctgttggtg aatacgcatg gcttcaccac
    10081 gggtgatgat tctcatgatg tgatacctct gaagacagaa gataaaagtg aaaacaggtg
    10141 tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa cctggttgcc
    10201 accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct gtaccagacg
    10261 ttcggtgata agccggtctg ccttactggg tgtgacttca ccggacgggt gattgtgcgc
    10321 cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc ggggatgaac
    10381 ttccgtacgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct gattctggtt
    10441 gttcagatac agcacccgga actcttcccg ctccagtccc gccatgttca gaatcagcca
    10501 ttcccgtgcc gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat ggcggtccag
    10561 ggtttgcagg gcccgcagaa tgagactgcg ctcgccgggc gtcatgtttc cgggcagaaa
    10621 ggaaagctgc tgcattgtcc ctgcctccat tcagtcgatg atgcgcataa tggcgctgca
    10681 ttccggatgc tgcagggcgt aatcccgcag ccggtaataa tggaccgtca tggcataaca
    10741 ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatgc cggcggcttc
    10801 cgggctcatt tcagcgcggt taccgttcat ggcattgaac agtacccatg tttcgtcatc
    10861 atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt acagattcca
    10921 gataccaccg cagtagtctt cacacagacg gtccatccag ccgaagacac ggggttccag
    10981 ggtgacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc gctgttcatc
    11041 agggacggga gtggcaacca gttgttgcag gccagtctcc ggtgcacagg ccgaagcggt
    11101 ggtgttctga gacaatgttt tcatgtatga aatccttgta atgtgaataa atgagaggga
    11161 gtaaaggtcg ggagctggca acatcacgtt catcactgac cgtgatgacc cggtaatatc
    11221 agggatgaat gatattcggc tgaaattccg gacagccgta cgcgtaaccg ttccggtcac
    11281 agacggatgg ccagatgcca ttgcctgtat actcagtctg cacagactga tttctccggg
    11341 acggtaaagc agacgggtac accaggcgca caggtcatca tcagaaatgg aacgacaccc
    11401 cggcagcgcc tccggggcgt cgggtgattc tggtgcctga atcacgcctt cagggacatg
    11461 ctgcctcagc gcaggctctc cagcagcgtt tctgccatta cccacaatgc gcggttgagc
    11521 ttaatatcgg tgtcgatgtt gtgaatggca cgggtgtgga tacgttttcc ttttgcactg
    11581 cggccggaaa tcccgccttt cagcatattc tcctggatgg tctgatatgc gctccacagg
    11641 tctttaccgt aatcctccct gcgtcgtggt gtcagaatgt cagcggtggt gactggctga
    11701 tgttcgtcac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc
    11761 ggcaggacca gcgactgcat ggcatcacgc ttctcctcta tccggtcaaa cacgcccacc
    11821 acctcgtaag ccccttcaat aactttctcc accacatttc cccggtgtgg aacacgcaat
    11881 tctcccagag actgaccgca gaccagtcca ttggtacaaa tggcacgaaa atatccgggt
    11941 aacatctggt agctggatga accgtcatga gagttgagca gaataatttc agggacatgc
    12001 tgaccggtta tctgtccggc ccgccgcaga cgcagcatgt gttttgtgta ttcccggcga
    12061 cccgggtcac gcacacgtgt ctggcaggcg aagaatggct gaaagccttc ccgctgcagg
    12121 ctttccagta cggtgatggt gggagtgtac gcatagcgtt tactgcggga agtatgccgg
    12181 tcttcaccga aaatactggg aacatagtgc atcagttctt cgtgtgtcag cggacggtca
    12241 cggcgtatct ggtttgcagc ataaccaaaa cgactggcta gtcgcataat ttgctcctta
    12301 tcggtggtta agatttactg gtgtaataaa tgaaaaagcc acgtctcccg gagaagacgc
    12361 ggcctgatgg acgaaattaa tgaggtttat tgtctgagaa gaccgtcact ggcgaacctt
    12421 ccggcgtcag tctctgcgtt gcggataacc cgacggaaat gagttccctg gtcatcgtcg
    12481 ataaacacat tgcggtaagc tgttgtgatg acaacagcct gctcgcgtga gaaagggact
    12541 tcgggcaata aacgtgacat acaaccagac ctgtatttgg ttccctgaaa atataagtcc
    12601 ctgtcatcat ggcagagccg tatttacttt tgatatcaat aaattaataa cgcgaagatg
    12661 aaccgcataa cttacgcctt taatttgttt tgtttacgta gtccctgacc aggtacgcga
    12721 attcgtgaac gccagtttca ccaaaccaaa tcaggtaacc agggtcgatg ttgtaatagt
    12781 aaccaacgac tttatgcgtt tttgggttat agagtgttac gatatagtcc cagccgcagg
    12841 cgtgggcttt acaaccgcca ccttctaccc aggtcgttcc gttaatgttt acaggatacg
    12901 caccaccatc aggaccatcc atatagccgt cttccccaaa cattccgttt aacccaacag
    12961 ggtttaatgc tttgtcgata gcagctctgt aggtagcgtc accttcgtat aactcgtacg
    13021 ttgttggaca tgcaccaggc tggtcagccc gggcttttga gtcgcattta agtgtctgat
    13081 ttacagtggc ggcgtttgtg tcaggactt
//
LOCUS       DAEBXD010000102        13015 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEBXD010000102 DAEBXD010000000
VERSION     DAEBXD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.102"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..319)
                     /locus_tag="KJL94_003207"
     CDS             complement(<1..319)
                     /locus_tag="KJL94_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD4411456.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="KJL94_003208"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="KJL94_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KJL94_003209"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KJL94_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBD4411457.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KJL94_003210"
                     /pseudo
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KJL94_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="KJL94_003211"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="KJL94_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBD4411458.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="KJL94_003212"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="KJL94_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBD4411459.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="KJL94_003213"
                     /pseudo
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="KJL94_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="KJL94_003214"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="KJL94_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="KJL94_003215"
     CDS             10613..11503
                     /locus_tag="KJL94_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBD4411460.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="KJL94_003216"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="KJL94_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBD4411461.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12493..12569)
                     /locus_tag="KJL94_003217"
     tRNA            complement(12493..12569)
                     /locus_tag="KJL94_003217"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="KJL94_003218"
     CDS             12812..>13015
                     /locus_tag="KJL94_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBD4411462.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3183 a         3373 c         3307 g         3152 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgag aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAEBXD010000103        12907 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEBXD010000103 DAEBXD010000000
VERSION     DAEBXD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.103"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KJL94_003219"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KJL94_003219"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBD4411463.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="KJL94_003220"
     CDS             complement(1806..3224)
                     /locus_tag="KJL94_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD4411464.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="KJL94_003221"
     CDS             complement(3378..3722)
                     /locus_tag="KJL94_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBD4411465.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KJL94_003222"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KJL94_003222"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBD4411466.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="KJL94_003223"
     CDS             complement(4712..5647)
                     /locus_tag="KJL94_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBD4411467.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="KJL94_003224"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="KJL94_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBD4411468.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="KJL94_003225"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="KJL94_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBD4411469.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KJL94_003226"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KJL94_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBD4411470.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="KJL94_003227"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="KJL94_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBD4411471.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="KJL94_003228"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="KJL94_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBD4411472.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="KJL94_003229"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="KJL94_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBD4411473.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KJL94_003230"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KJL94_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBD4411474.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="KJL94_003231"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="KJL94_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBD4411475.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="KJL94_003232"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="KJL94_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBD4411476.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3296 c         2951 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAEBXD010000104        12695 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEBXD010000104 DAEBXD010000000
VERSION     DAEBXD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.104"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="KJL94_003233"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="KJL94_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBD4411477.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="KJL94_003234"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="KJL94_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBD4411478.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="KJL94_003235"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="KJL94_003235"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBD4411479.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="KJL94_003236"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="KJL94_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBD4411480.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="KJL94_003237"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="KJL94_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBD4411481.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="KJL94_003238"
     CDS             4025..4615
                     /locus_tag="KJL94_003238"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBD4411482.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="KJL94_003239"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="KJL94_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBD4411483.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="KJL94_003240"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="KJL94_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBD4411484.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="KJL94_003241"
     CDS             complement(6551..6658)
                     /locus_tag="KJL94_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411485.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="KJL94_003242"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="KJL94_003242"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD4411486.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="KJL94_003243"
     CDS             7648..7875
                     /locus_tag="KJL94_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBD4411487.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KJL94_003244"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KJL94_003244"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBD4411488.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="KJL94_003245"
     CDS             complement(9447..10703)
                     /locus_tag="KJL94_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBD4411489.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="KJL94_003246"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="KJL94_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBD4411490.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="KJL94_003247"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="KJL94_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBD4411491.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KJL94_003248"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KJL94_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD4411492.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3244 c         3198 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAEBXD010000105        12494 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEBXD010000105 DAEBXD010000000
VERSION     DAEBXD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.105"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            420..2060
                     /gene="trg"
                     /locus_tag="KJL94_003249"
     CDS             420..2060
                     /gene="trg"
                     /locus_tag="KJL94_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBD4411493.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2098..3021)
                     /locus_tag="KJL94_003250"
     CDS             complement(2098..3021)
                     /locus_tag="KJL94_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4411494.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3238..4581
                     /locus_tag="KJL94_003251"
     CDS             3238..4581
                     /locus_tag="KJL94_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD4411495.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4806..6461
                     /gene="opgD"
                     /locus_tag="KJL94_003252"
                     /gene_synonym="mdoD"
     CDS             4806..6461
                     /gene="opgD"
                     /locus_tag="KJL94_003252"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBD4411496.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6601..6825
                     /locus_tag="KJL94_003253"
     CDS             6601..6825
                     /locus_tag="KJL94_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBD4411497.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6888..7424
                     /gene="rimL"
                     /locus_tag="KJL94_003254"
     CDS             6888..7424
                     /gene="rimL"
                     /locus_tag="KJL94_003254"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBD4411498.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7419..8399)
                     /locus_tag="KJL94_003255"
     CDS             complement(7419..8399)
                     /locus_tag="KJL94_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411499.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8523..9515
                     /gene="tehA"
                     /locus_tag="KJL94_003256"
     CDS             8523..9515
                     /gene="tehA"
                     /locus_tag="KJL94_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBD4411500.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9512..10105
                     /gene="tehB"
                     /locus_tag="KJL94_003257"
     CDS             9512..10105
                     /gene="tehB"
                     /locus_tag="KJL94_003257"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBD4411501.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10408..11076
                     /locus_tag="KJL94_003258"
     CDS             10408..11076
                     /locus_tag="KJL94_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBD4411502.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11094..11600)
                     /gene="tnpA"
                     /locus_tag="KJL94_003259"
                     /pseudo
     CDS             complement(11094..11600)
                     /gene="tnpA"
                     /locus_tag="KJL94_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11608..12494
                     /locus_tag="KJL94_003260"
                     /pseudo
     CDS             11608..12494
                     /locus_tag="KJL94_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3254 a         2921 c         3135 g         3184 t
ORIGIN      
        1 acgacttcaa ggcgcttttc aaaagggtat tttggctttg acatattagg ggctattcca
       61 tttcatcgtc caacaaaatg ggtgcagtac agttgataga aatatcggtc ggggccttcg
      121 tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca ggcatcctat
      181 gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt ttccagaatt
      241 tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc aatagcatta
      301 attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg tcataagtaa
      361 ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag acaacggtaa
      421 tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg gttccgttat
      481 ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct ctggctggct
      541 atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt gagattcgga
      601 ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg attcaggccg
      661 gggctgcgag tcgtattgcg gaaatggaag caatgaagcg aaatattgcg caagccgaat
      721 cggagattaa acagtcgcag caaggttatc gtgcttatca gaatcgaccg gtgaaaacac
      781 ctgctgatga agccttcgac actgaattaa atcaacgctt tcaggcttat atcacgggta
      841 tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc aatcatgaaa
      901 gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa gccgttaaga
      961 tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc cgcctgggtg
     1021 ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata acatttatgg
     1081 tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt gaaaaaatcg
     1141 ccagtggcga tctgacgatg aatgatgaac cggcgggtcg taatgaaatc ggtcgcttaa
     1201 gtcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact gttcgacagg
     1261 gcgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg gacctgtcat
     1321 ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg gagcaactca
     1381 ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg gcgcaagagg
     1441 cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa acgatgggcg
     1501 ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac agtattgctt
     1561 tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag
     1621 ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagccgc agcgctcagg
     1681 cagcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac ctggggtcgg
     1741 atgaggtggc aacggcaggg aaaaccatga gcactattgt tgatgccgtc gcgagtgtca
     1801 cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc ataactcagg
     1861 ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct ctggtagaag
     1921 aggcctcagc agcggcggtg tcccttgaag aacaggctgc acgattaact gaggcggtga
     1981 acgtattccg tctgaacaaa cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag
     2041 ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct tcacctttca
     2101 gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag agaacgtcgc
     2161 atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa caggcagggc
     2221 caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa accacacgta
     2281 atcgtattca acggtaagtt gacgagatag cgaagtagac agcgtttcga tacaacccga
     2341 aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg gcgcagtgcc
     2401 ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt tctcctgaag
     2461 tagcgggtga ttagggcgga caaccagctt caacgattca agaaacagca gttcgtaatt
     2521 aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga tttccccggt
     2581 ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg tcgtctcttt
     2641 ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg cagtaggtag
     2701 tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg actgtccggc
     2761 agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt
     2821 aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct gctccagttc
     2881 attcaatgta ttagagagcg caggttgact caaattaagg gtttcagctg cgcgccccaa
     2941 agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga tgcgctgact
     3001 aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc tgacaagtga
     3061 agttgtttga ttatgataac ttgattgcaa aatattatta ataattaaag caattatgtt
     3121 acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta aattcttccc
     3181 gccagagtga atgcggttac ctacactcca gattactgac cactggaggc agacactatg
     3241 gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat gtcagccatg
     3301 taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga tgtgaatctg
     3361 gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga cgagctggcg
     3421 cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga gcttgctact
     3481 cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga tctctcgcag
     3541 gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc tctggcgcgt
     3601 aatcccttcc gcgtttttac atccttactc cgccttgagc ttatcgagaa cgaaattttg
     3661 cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg ttgtcgacaa
     3721 ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca ggagtttgtg
     3781 caggaagccc tggaaacgtt tcgctggcac cagttagcaa cggtagatga agaaacctat
     3841 cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc tggatgccat
     3901 atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat gacgcctgaa
     3961 tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt accgatttta
     4021 ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg gcagaaacag
     4081 ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt aacgccgaaa
     4141 gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga taatctcact
     4201 caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt tttaatgcgt
     4261 cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc gcatcgtcag
     4321 gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt agtggcgcaa
     4381 cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca gtcaaatctg
     4441 ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt tgagcaggcg
     4501 ttgggttgtc cggttttgga tgagttccag ctttaccagg aagcggaaga acgcagtaaa
     4561 cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt gcctgatttt
     4621 acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg catattctct
     4681 cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat aaataagcac
     4741 tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag gactcacttt
     4801 caggtatgga tcgtagacga tttattaaag gtttaatggc tatggccgcc gtgtgcggta
     4861 ccagcggcat tgcttctctt ttttctcagg cagcattcgc ggcagattct gatattgccg
     4921 acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac gacttagcgc
     4981 aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca atgacgccgc
     5041 aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac gttgagaacc
     5101 gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc gttcgtatgt
     5161 tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg gagttgttca
     5221 aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat ctcggctttg
     5281 ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta tcatttctcg
     5341 gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg gcccgcggcc
     5401 tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc gccttctggt
     5461 ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc gatagcgcca
     5521 gcattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg attatggatg
     5581 tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcatttcg ccgatgacca
     5641 gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga tacaattcat ccgcaaattc
     5701 atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc cgtccgctga
     5761 ataatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa gggtttggtt
     5821 tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg tataacaaac
     5881 gcccaagtct gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc ggcctgatgg
     5941 aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag ccagaaaaag
     6001 ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt gcgcaaccgc
     6061 ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc ggtttcccgg
     6121 aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt gccgtcgatt
     6181 tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg attacgcttt
     6241 ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat ggttatcgta
     6301 ttcagtttga ctggtatccg acttcggact ccactgatcc ggtcgatatg cggatgtatc
     6361 tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc ccgccagcgc
     6421 cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt cttcggcacc
     6481 ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc ctaagttaaa
     6541 tgagtaatct gatggtgtgt atttcagata caccgtgtca gccactaaca gggagtgcgt
     6601 atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc tcgctttatg
     6661 tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga aggttccgac
     6721 ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca gttaaaagat
     6781 gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt cctaagccgc
     6841 cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg actgaaacga
     6901 taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc acacctcttt
     6961 atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg ccgcagttcg
     7021 ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg catcaacgcg
     7081 gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt atctcgttta
     7141 atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac gaatctcatc
     7201 aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac gctcagtctg
     7261 gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc aaccaggtcg
     7321 ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc ctgaatgatg
     7381 cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc tgccagcggc
     7441 gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg aaccgtgtcg
     7501 ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc aattatcacc
     7561 gcacctgtaa tacggctctc accttgtatt acaacgtgct cgtcgagcag aatcggtcca
     7621 ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca attaccttcc
     7681 acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc atcttcttct
     7741 atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag ccagacgttg
     7801 ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg ttcaataaaa
     7861 gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg cgaggcgcta
     7921 acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa gccttgtgcg
     7981 gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca ttgcacgtaa
     8041 actaatgagt catgaatggt gacactgtca cttatatatg caccctggct gatttcacta
     8101 ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct ggtgccggtg
     8161 atacgcgtat taccggaaat caccgttccg ccgaacgcaa tagcattctg gtcgtaaatc
     8221 cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc agcggttcct
     8281 gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac acttttttta
     8341 gtgccatctt cctgataact aaaggctcgc tgttcttcac ttaggcgata tttgcgcata
     8401 gcgttttccc acaggtgact tactataacc gtagcaaatt ctgcggctct ggctatgctc
     8461 gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa atgagtaaga
     8521 agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt gtgttgggga
     8581 cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc agccactggt
     8641 taggggatgg gctggtgatt ctggcgatga tcatctgggg attattgact agcgcattta
     8701 ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat ccagtgctga
     8761 gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt tttgttccgt
     8821 ggtttcgccc actggcggtg tgcatgttca gttttggtgt cgtggttcag ttggcttatg
     8881 ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct accacgcctg
     8941 gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt ggtgcgttgg
     9001 gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg ctaagccttg
     9061 aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca ctgcggacat
     9121 cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggctg agcgtcaacg
     9181 gcggcgaggg tgacacgctg gcgaaaatgc ttttcggtta tggactgctg caactgctgt
     9241 ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca ttctggagtt
     9301 tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt ggcagcgata
     9361 atggattttt ccatacgctg gcggtgccgc tgtttatctt taccaatttt attattgcaa
     9421 tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc agaaccgagc
     9481 gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa actattttac
     9541 tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga aagtggttaa
     9601 accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt acctggcagc
     9661 caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca acgtcgagcg
     9721 cattaaatcc attgaaaatc tggataattt acacacccgg gtcgttgatc tgaataacct
     9781 cacatttgat ggacagtacg attttattct ttcgactgtg gtgctgatgt tccttgaggc
     9841 taaaaccatc ccagggttga ttgccaatat gcaacgttgc actaaacctg gtggttacaa
     9901 cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct tcacgtttgc
     9961 cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga aatacaatga
    10021 agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac tgcgtttcgc
    10081 cacgatgctg gcacgtaaaa aatgacccgg taagcacaaa acgcgtgaaa attccccacg
    10141 ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag ggatgtgaaa
    10201 ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa ctactgatga
    10261 ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg aaaaatttat
    10321 tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata acttagaaag
    10381 taatataatc atctcaggaa actattcatg cgtaccacat catttgcgaa agttgcagct
    10441 ttatgcggct tgttggctct gtctggttgt gcctctaaaa tcacccagcc agataaatat
    10501 tctggttttt taaacaatta ctctgattta aaagaaacaa cctcagcaac aggtaaacct
    10561 gttttacgtt gggtagaccc gagttttgat caaagcaaat atgacagcat cgtctggaac
    10621 ccaatcactt attatccggt accaaaacct tctacccagg tagggcagaa agttctggat
    10681 aaaattttga actataccaa caccgaaatg aaagaagcga tagcgcagcg taaaccagtg
    10741 gttaccaccg ctgggccgcg tagtctgatt ttccgtgggg ccattaccgg tgtagatacc
    10801 agcaaagaag ggctgcaatt ctatgaagtg gttcctgttg cattagtggt tgcggggacg
    10861 caaatggcta caggccaccg taccatggat actcgcctct attttgaagg tgagctgatt
    10921 gatgcagcga ctaataaacc ggttatcaaa gtcgttcgtc agggcgaagg taaagacctg
    10981 aataacgaaa gtacgccaat ggctttcgaa aatattaaac aagttattga tgacatggcg
    11041 accgatgcca ccatgtttga cgttaacaaa aagtagttcc agacgcgcca tcgttagatg
    11101 gcgcttttta tccggtgcgc cgtaaaaccc catccttcag ggcggggata taaggcgcgg
    11161 ttttccacct gaccaggtgt ttgctgttgt tcaatgtatt ggcggatgac ggatattggc
    11221 gcacctccgc agctactggc aaaatagcca ggactccaca aaacgccttt gtagtaatac
    11281 ctgactgcaa tatctggtcg atctcgtcgc agtaacctac ccgatacgcc tttgaggctg
    11341 tttaccagac tggatatcgc cagtttggga ggttagttaa ttaacaaatg gacgtgatct
    11401 ggttcgccat ccatttcaac cagttcagct tcaaaataag cacatacatt tgaaaagtaa
    11461 gtgcgtagtt tttctgtcgc gtcatggtca aaaatctggc gtcggtatct ggtgacaaag
    11521 actaggtgaa catgcatcag gaaaacacaa tgccttccac gccggatatc ggtttctttt
    11581 ttcacagacc aaagtatcat ttgacctgtg aaacgattac aggcatttaa attccagtta
    11641 agaccaggtg gtcaacagga gtgtgaaatg aggcgcttcg caggcgcatg tcgtttcgtt
    11701 ttcaatcgtg ctctggcacg tcagaatgag aatcatgagg ccggtaataa atacatccct
    11761 tacgggaaaa tggcttcctg gctggttgag tggaaaaatg ccactgaaac gcaatggctt
    11821 aaagattctc cctcacagcc attgcaacag tcactgaaag accttgagcg ggcttacaaa
    11881 aacttcttcc ggaagcgggc tgcttttccc cgattcaaaa agcggtgaca gaatgatgca
    11941 ttccgctacc cgcagggcgt taagctcgat caggaaaaca gccgtatttt tctgccgaaa
    12001 ctgggctgga tgcgctaccg gaatagccgt caggtcacgg gtgttgtgaa aaatgtcact
    12061 gtcagccagt cctgcggtac gtggtacatc agtattcaga cagaaagtga agtatccact
    12121 ccggctcacc cttcagcatc aatggtcggg ctggatgctg gcgtggctaa actcgccacg
    12181 ctgtcagatg gcacagtctt tgagcctgta aacagttttc agaaaaacca gaagacgctg
    12241 gcgagacttc agcgacagtt aagccgcaag gtcaaattca gcaacaactg gcagaagcag
    12301 aaacgcaaaa tacagcgact gcattcctgt atcgcaaata tccgcaggga ctaccttcac
    12361 aaagtcacaa cggccgtcag caaaaaccac gcaatgatag tcattgagga tttgaaggtc
    12421 agcaacatgt caaagtcagc agcgggtacg gtcagccagc cggggcgcaa tgtccgggca
    12481 aaatcaggtt taaa
//
LOCUS       DAEBXD010000106        12413 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEBXD010000106 DAEBXD010000000
VERSION     DAEBXD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.106"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..1198
                     /gene="edd"
                     /locus_tag="KJL94_003261"
     CDS             <1..1198
                     /gene="edd"
                     /locus_tag="KJL94_003261"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBD4411503.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1235..1876
                     /gene="kdgA"
                     /locus_tag="KJL94_003262"
                     /gene_synonym="eda"
     CDS             1235..1876
                     /gene="kdgA"
                     /locus_tag="KJL94_003262"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBD4411504.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1932..3110)
                     /gene="purT"
                     /locus_tag="KJL94_003263"
     CDS             complement(1932..3110)
                     /gene="purT"
                     /locus_tag="KJL94_003263"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBD4411505.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3244..3534
                     /gene="yebG"
                     /locus_tag="KJL94_003264"
                     /pseudo
     CDS             3244..3534
                     /gene="yebG"
                     /locus_tag="KJL94_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3601..3957
                     /locus_tag="KJL94_003265"
     CDS             3601..3957
                     /locus_tag="KJL94_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBD4411506.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4284..4943
                     /locus_tag="KJL94_003266"
     CDS             4284..4943
                     /locus_tag="KJL94_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBD4411507.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5153..7213
                     /gene="ptrB"
                     /locus_tag="KJL94_003267"
     CDS             5153..7213
                     /gene="ptrB"
                     /locus_tag="KJL94_003267"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBD4411508.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7210..7872)
                     /gene="exoX"
                     /locus_tag="KJL94_003268"
     CDS             complement(7210..7872)
                     /gene="exoX"
                     /locus_tag="KJL94_003268"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBD4411509.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7896..8552)
                     /locus_tag="KJL94_003269"
                     /pseudo
     CDS             complement(7896..8552)
                     /locus_tag="KJL94_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8654..8884)
                     /gene="holE"
                     /locus_tag="KJL94_003270"
     CDS             complement(8654..8884)
                     /gene="holE"
                     /locus_tag="KJL94_003270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBD4411510.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9023..9397
                     /gene="yobA"
                     /locus_tag="KJL94_003271"
     CDS             9023..9397
                     /gene="yobA"
                     /locus_tag="KJL94_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBD4411511.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9401..10273
                     /gene="copD"
                     /locus_tag="KJL94_003272"
                     /pseudo
     CDS             9401..10273
                     /gene="copD"
                     /locus_tag="KJL94_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10286..10627
                     /locus_tag="KJL94_003273"
     CDS             10286..10627
                     /locus_tag="KJL94_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBD4411512.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11020..12096)
                     /locus_tag="KJL94_003274"
                     /pseudo
     CDS             complement(11020..12096)
                     /locus_tag="KJL94_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12062..12343)
                     /locus_tag="KJL94_003275"
     CDS             complement(12062..12343)
                     /locus_tag="KJL94_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBD4411513.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3079 a         3032 c         3137 g         3165 t
ORIGIN      
        1 ggccttactg gagtcagaag ccgcgtctta ccatgcgccg ggaacatgta ctttctacgg
       61 tactgccaac accaaccaga tggtggtgga atttatgggg atgcagttgc caggctcttc
      121 ttttgttcat ccggattctc cgctgcgcga tgctttgacc gctgccgctg cgcgtcaggt
      181 tacacgcatg accggtaatg gtaatgaatg gatgccgatc ggtaagatga tcgatgagaa
      241 agtggtggtg aacggtatcg ttgcactgct ggcgaccggt ggttccacta accacaccat
      301 gcacctggtg gcgatggcgc gcgcggccgg tattcagatt aactgggatg acttctctga
      361 cctttctgat gttgtaccgc tgatggcacg tctctacccg aacggtccgg ccgatattaa
      421 ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt gaactgctca aagcaggcct
      481 gctgcatgaa gatgtcaata cggtggcagg ctttggtctg tctcgttata cccttgaacc
      541 atggctgaat aatggtgaac tggactggcg ggaaggggcg gaaaaatcac tcgacagcaa
      601 tgtgatcgct tccttcgaac aacctttctc tcatcatggt gggacaaaag tgttaagcgg
      661 taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg gttgagaacc aggtgattga
      721 agcgccagcg gttgtttttg aaagccagca tgacgttatg ccggcctttg aagcgggttt
      781 gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg ccaaaagcga acggaatgcc
      841 agaattacat aaactcatgc cgccacttgg tgtattattg gaccggtgtt tcaaaattgc
      901 gttagttacc gatggacgac tctccggcgc ttcaggtaaa gtgccgtcag ctatccacgt
      961 aacaccagaa gcctacgatg gcgggctgct ggcaaaagtg cgcgacgggg acatcattcg
     1021 tgtgaatgga cagacaggcg aactgacact gctggtagac gaagcggaac tggctgctcg
     1081 cgaaccgcac attcctgacc tgagcgcgtc acgcgtggga acaggacgtg aattattcag
     1141 cgccttgcgt gaaaaactgt ccggtgccga acagggcgca acctgtatca ctttttaaga
     1201 cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa aactggaaaa caagtgcaga
     1261 atcaatcctg accaccggcc cggttgtacc ggttatcgtg gtaaaaaaac tggaacacgc
     1321 ggtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc gttctggaag tgactctgcg
     1381 taccgagtgt gcagttgacg ctatccgtgc tatcgccaaa gaagtgcctg aagcgattgt
     1441 gggtgccggt acggtgctga atccacagca gctggcagaa gtcactgaag cgggtgcaca
     1501 gttcgcaatt agcccgggtc tgaccgagcc gctgctgaaa gctgctaccg aagggactat
     1561 tcctctgatt ccggggatca gcactgtttc cgaactgatg ctgggtatgg actacggttt
     1621 gaaagagttc aaattcttcc cggctgaagc taacggcggc gtgaaagccc tgcaggcgat
     1681 cgcgggtccg ttctcccagg tccgtttctg cccgacgggt ggtatttctc cggctaacta
     1741 ccgtgactac ctggcactga aaagcgtgct gtgcatcggt ggttcctggc tggttccggc
     1801 agatgcgctg gaagcgggcg attacgaccg cattactaag ctggcgcgtg aagctgtaga
     1861 aggcgctaag ctgtaaaagt caaaatgccc gatctaggat cgggcatttt tgtagcgttt
     1921 ttttgccggg tttaaccctg tacttttacc tgtccggcgg cgtgcttcgc gcgttcaatg
     1981 gcgtcaacaa cactctctga agtagccagt accaccccca gacgacggct gccatcaatt
     2041 tccggcttac caaataaacg aatctgcaaa tctgcgccta cggcattctg cacattatca
     2101 aacgtgacat tctgactggt cagttgtggc agaataacgg cagaagctgc aggaccatac
     2161 tgacggatcc cgccaacagg aagtccgagg aaggcacgta catgcagggc aaactctgag
     2221 agatcttgag aaattaacgt caccatcccg gtatcatgtg gacgagggga gacctcactg
     2281 aaaatcacct catcaccaca gacaaacagc tcgacaccaa acagaccata accgcccagt
     2341 gccagcacca ctttacgggc aatctcctgc gcacgttcaa gggcaagcgg gctcatttgc
     2401 tgtggttgcc aggattcacg gtagtcgcca tcttcctggc gatgacctac tggtgcacag
     2461 aaatggacgc catccaccgc gctgacggtt agcagggtaa tttcgaagtc aaacttaacg
     2521 acgccttcaa caattacgcg gcccgctccg gcgcgaccgc cttgctgagc gtattcccat
     2581 gcctgagcaa gttgctctgc agagcgaata aacgtctgcc ccttgccgga agagctcatc
     2641 accggtttta caatgcaggg atagccaatg gcggcaaccg cctcgcggaa aaggcttttg
     2701 ctatcggcaa aacgataagt ggaagtgggc agctgcagct cttctgccgc caggcgacgg
     2761 ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc aggggacaac attcagtcct
     2821 tcctcttcaa gttggatcag catatcggtg gcaatagctt cgatctccgg cacgatataa
     2881 tgtggttttt ccagttcaac cacacggcgt aatgcatcac catcaagcat attaatgaca
     2941 tgggagcgat gcgcgacatg catggctggt gcgtcggcat agcgatcgac ggcaatcacc
     3001 tctacgccga gacgctgaca ctcgattgcc acttctttac ccagttcacc ggagcctaat
     3061 aacatcacgc gagttgctgc cggacgcagc gcagtgccta ataacgtcat aacgaatatc
     3121 ccctgattaa ttccgctcgc agtataaacg aaaacgtttg cgtgtgtctt tattgctgat
     3181 gttgatttct caaccgaaaa gaaatatact gtataaaatc tcagttatta tgagaggctg
     3241 caaatggcgg ttgaagttta atacgtagtc attcgtgagg gcgaagagaa aatgtcgttt
     3301 accagcaaaa aggaagccga tgcttatgac aaaatgcttg atacggcgga tctgcttgac
     3361 acctagctga caaattctcc agtgcaaatg gaagacgagc aacgtgaagc cctttcgcta
     3421 tggctggcag aacaaaaaga ggtgctgagc accattctga aaaccggcaa attaccgtcc
     3481 ccacaggtcg tcggtgctga atccgaagag gaagatgcct cacacgcggc ctgaagcaac
     3541 ttgcgctcct gacaatttgt accatgcttt ctcctgaatc attgtgctat ggagaaaaac
     3601 atgaaaaaaa gaggggcgtt tttagggcgg ttgttggttt ctgcctgcgc atcagttttc
     3661 gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg tctggatgct
     3721 gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa atcgcgtggc gcgctgggca
     3781 gatgatcaaa aaattgtcgg tcaggccgat cccgtggcct gggtcagttt gcaggacatt
     3841 cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag tgccgatatt
     3901 cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg aatatcagcg gcgttaatgg
     3961 cttctgataa ttcgattgac cgttggcgga tgcggcgcga gtgccttgtc cgtcccagaa
     4021 cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg catcgggcaa tcagcatcaa
     4081 acaatgccac tcaaaacgac aaactacttt cacctaaaat acccttatca cttgatatac
     4141 agtaaatacc ttacctggcg cgaatttctt tgaataacgg cctaccgccc cgcttatttg
     4201 cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct acaggcacta
     4261 aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa tccctgcttg
     4321 ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg attgggcaaa ctgttagtgt
     4381 caggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca cgtaaacttc
     4441 ttacgaaata tggcaccaac gcgttactgg ttggcggcgg agcggtagcg ggtacggtgc
     4501 tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg cagtttggcg
     4561 cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg gtctttgccg
     4621 ctaaaagtga tggtcatatt gatgccaatg aacgtgcggc aatcgaccag caattgcgtg
     4681 aagccggcgt ggaagagcag gggcgtgtac tcattgagca ggcaatcgaa caaccgctgg
     4741 atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa atctatttcc
     4801 tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg aatgcactgg
     4861 gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat tgaacgcgat ctcgaacagc
     4921 aaaaacgcac tttggcggaa taattccgct ctctggatag cctgaagttg actatttcgg
     4981 gctatccaga accgcacagc catttgaaca ggctgatttt ctctgtctgc ataacattcg
     5041 atatccgcgc cattacctgt catcatctaa gcaatgacga ccctgtttcg cttgcatccc
     5101 cggtgagttt tgccaccctt ataagatgtt tccaaccaga aagaacaata acatgctacc
     5161 aaaagccgcc cgcattcccc acgccatgac gcttcatggc gatacgcgca tcgataatta
     5221 ctactggctg cgggacgata cgcgttctca gccaaaagtc ctggactacc tgcaacaaga
     5281 aaatagttac ggtcatcggg tgatggcctc acaacaagcc ttgcaggatc gcatcttaaa
     5341 ggaaatcatc gaccgcattc cgcaaagaga agtttctgcg ccctacataa aaaatggcta
     5401 ccgctatcgg catatttatg aaccaggctg tgaatatgct atctaccagc gtcaatcggc
     5461 attcagcgaa gagtgggatg agtgggaaac attgctcgat gccaacaagc gcgcagctca
     5521 tagtgagttt tattcgatgg gcggaatggc gattacgccc gataacacca ttatggcgct
     5581 ggcagaagat tttctttccc gacgccagta cggcattcgt tttcgtaatc tggaaacagg
     5641 taactggtac ccggaactgc tggataacgt tgaacccagc tttgtctggg caaatgactc
     5701 ctggactttc tactatgttc gcaagcatcc ggtgacgctg ctgccttatc aggtctggcg
     5761 tcacgccatc ggtacgccag catcgcaaga tacactgatc tacgaagaaa aagacgatac
     5821 ctattatgtc agcctgcata aaacgacgtc gaagcactat gtcgtcattc atctggccag
     5881 cgccaccacc agtgaagttc gcctgctgga cgcggaaatg gccgatgccg agccgtttgt
     5941 ttttctgccg cgccgcaaag atcacgaata cagccttgat cactaccagc atcgttttta
     6001 tctgcgttcc aatcgccacg gcaaaaactt tggcttatac cgtacccgta tgcgtgatga
     6061 gcaacagtgg gaagagttaa ttccgccacg cgaaaacatc atgctggaag ggtttacgct
     6121 gtttaccgac tggctggtgg ttgaagagcg tcagcgcggg ttaaccagtt tgcgccaaat
     6181 taaccgcaag acccgggaag tcattggcat tgcctttgat gatccggcct atgtgacctg
     6241 gattgcctac aatccagaac ctgaaaccgc gcgattgcgt tatggttatt cttccatgac
     6301 cacaccagac actttgtttg aactggatat ggataccggt gagcgtcgtg tattaaaaca
     6361 aacagaagtg cctggttttg atgcggcgaa ttaccgcagt gaacacctgt ggatagtcgc
     6421 ccgtgatggc gtcgaagttc cggtttcgct ggtctatcat cgcaaacatt ttcgcaaagg
     6481 acacaacccg ttgctggtgt atggctatgg ttcttacggc gcaagtattg atgccgattt
     6541 cagttttagc cgcttgagtt tgttagatcg tggctttgtc tacgccattg tccatgttcg
     6601 cggcggtggt gagctggggc aacaatggta cgaagacggt aaatttctga agaagaaaaa
     6661 tacgtttaat gattatcttg atgcctgcga tgcattgtta aaactgggct atggctctcc
     6721 ttcgctctgt tatgcgatgg gcggaagtgc ggggggcatg ttgatgggcg ttgcaattaa
     6781 tcaacgcccg gaattattcc acggcgttat cgcccaggta ccgtttgttg atgttgtaac
     6841 aacgatgctt gatgaatcaa ttcctcttac cactggtgag tttgaagagt ggggaaatcc
     6901 gcaggatccg caatattacg agtacatgaa aagctacagc ccgtatgaca acgtcaccgc
     6961 acaggcttat ccgcatttac tggtaacgac cggtttacac gattctcagg tgcaatattg
     7021 ggaaccggca aaatgggtcg ctaaattgcg cgagctgaaa accgatgacc atcttttatt
     7081 gctctgtacc gacatggact caggccatgg cggtaaatct ggccgcttta aatcgtacga
     7141 aggcgtagcg atggaatatg cttttctggt cgcgctggcg cagggaacat tacccgctac
     7201 gcctgcggat taagtatttt ccagataatg tttcagtgtt aaacgcagct ccgggctcat
     7261 gctgttcagg ttattaaata accagcgcag atagcccgga tagcgttcgg caacgtcgga
     7321 aaccgctttg ccacggtatt tgccaaaggt gaaggtcgtc atcaacgacg gacgtccggt
     7381 gatatcggcc atctgttctg ccgtccagcc ggaggtgttc ataatatcga taagcaacgc
     7441 ggcggtgata taacagtcat acaacgcgcg gtgatgatgc aggcccggcg gtgtctgtac
     7501 attgagcttg cgtgttttat ataacgccat attgctgtac ttgagccctg gccacaaacg
     7561 acgggccagt ttcatagtgc aaatccactc accgggcatc tcaggcagta cgcggcggtc
     7621 aaagctggcg ttgtgcgcga cataccattc actaccgtaa tagtgtggga tcacatcttc
     7681 aatccacggt ttatcggcga ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg
     7741 actaatagga cgatcggggc gcaccaggag gctcatgggg ttgacgattt ttccgtcaat
     7801 gacatcaaca gaggcaatct caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat
     7861 aatgcgcaac atggcatgct ccaggccgaa aaagcctagc gtaatggaat gatatcgcct
     7921 tgccaaccct gtgatgaacg ctgaccgact aacaataatg aaccacgatc ggcgcgaacg
     7981 atgagacgac catattcatc ccatagtgca ctgttaccac gggcatttgc catcagcacg
     8041 gcaatcgagt actgatgtga aaaaaattgt aagcgacggg ccgacgccag caggtcgggt
     8101 tctcccagac attgccctgt cgtaaacagt gaacaggtcg ggtccatatc cataccttgc
     8161 ggttgttcat cgaccaccgt gatggttctg gaacgacggc ccagacatgc accatgactc
     8221 tgatggtagt tcccaggcgt ttttcgccac ggtgcgaaca ccgcaattcc acgaataaag
     8281 cgatcgttat attcaacggg aaggccggca atgatggtca ttcgctaggt cgttgcggca
     8341 taacaaagcg ggtctaacag tgaaagatcg ggtggggcag gaagggcacg tcgcgaataa
     8401 tcacacccca gtaaagaaag cgatggaaaa actaacagct gacattgttg cctggcggcg
     8461 gccctcacaa actccagatg atgagctacc tgttcggtga gtgaggtttt acgaggctca
     8521 tattgcgctg ctgcaacttt ccagaacgac ataatgactt ccttttcaaa atgactgcaa
     8581 attatggggg cggtttagaa aatactcata atttagcagg cgttatgtaa gaaagtgtaa
     8641 ctctgtataa gttttattta agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag
     8701 ccaaacggtg ggcaataagc cgctcgcgaa accagctgcg caaatgttct ggctgttcac
     8761 gttcaaccgc ttcagcgatc accggcatat tgtagcgttc tttaaaagcc accccggccg
     8821 ccgccaaatc gacattcact ttatccattt ctgtttgatc cagtttagcc agattcttca
     8881 gcattcttaa tctcctcatc attcgcgggc aaaagttact aagagtgagc ctggatggca
     8941 agccggaatt gatgatcctc gactcagaca ttagacaagg ttatgctctg taaaaaagtt
     9001 attacaaaaa ggaatagata atatggcttc aactgcacgc tcccttcgct acgcgctggc
     9061 aatcctgacc acttcgttgg tgacaccttc cgtctgggca catgcgcatt taacgcatca
     9121 gtatcctgcg gcaaacgcgc aagtgacagc tgccccgcag gcaatcactt taaacttctc
     9181 ggaaggcgtt gaaacaggat tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat
     9241 taaaacatta ccagcgaagc ggaatgagca ggaccaaaag caactgattg ttccactggc
     9301 cgattcgctg aaacccggaa cctacaccgt tgactggcat gttgtttcgg tggacgggca
     9361 taaaacgaaa ggacactaca cctttagcgt gaaataaatc atgctggcgt ttacctggat
     9421 cgcgctgcga tttatccatt tcacctcgtt gatgctggtc tttggctttg caatgtatgg
     9481 tgcctggctg gcccccttaa tgattcgtcg cttgctgacg aagcgttctc tgcgtttaca
     9541 acagcacgct gccgtctgga gtttgatcag tgccactgca atgctcgccg ttcagggcgg
     9601 gttgatggga acgggatgga cagatgtatt ttcgccaaac atttggcagg ctgttttgca
     9661 aacgcagttt ggtggcgtct ggttatggca aattgttctc gccctcgtaa cgttgatcgt
     9721 cgcccttatg caaccgcgga atatgccacg tttgctgttt atgctcacta ccgcgcaatt
     9781 tattctgctg gcgggagtgg ggcatgcgac gctgaatgaa ggggtaacag cgaaaatcca
     9841 tcagactaat cacgcgattc acctgatttg tgcggcggcg tggtttgggg gattactgcc
     9901 ggtgctctgg tgtatgcaac tcatcaaagg ccgttggcga cactaggcta ttcaggcgct
     9961 gatgcgtttt tcctggtgcg ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct
    10021 taatgcattg ttgattaccg gatttccccc cacgcttacg acttactggg gccaactctt
    10081 attgctcaaa gccatcctgg tgatgattat ggtggtcatt gcgctggcta atcgttatgt
    10141 tcttgtaccg cgcatgaggc aggatgaaga tcgtgcggca ccctggtttg tgtggatgac
    10201 taaactcgaa tgggcgatag gtgcggtagt gctggtgatc atcagcctgc ttgcgactct
    10261 cgaacctttt tgatggatta cgaaaatgat gaaaaaaagt attctggcgt ttctgttact
    10321 caccagttct gcagcggcgc tggcggctcc acaggtgata accgtgagtc gttttgaagt
    10381 gggtaaagac aaatgggcgt ttaatcgcga agaggtaatg ctgacttgcc gaccgggtaa
    10441 tgctttgtat gtcatcaacc caagtaccct cgtgcagtat cctttaaacg atatcgcaca
    10501 aaaggaagtt gccagtggga agaccaatgc ccaacccatt tcggtgattc agattgatga
    10561 tcctaacaat cccggcgaaa aaatgagtct ggcaccgttt atagaacgag ctgaaaaact
    10621 ctgttaatta cctaaaatag ccttttgatt tccaataaaa aaaccgcctc agttctttca
    10681 ccagaacggg cggtttttaa catttaagct gatgagcacc gcgcttttta ttgaccattt
    10741 tgcacgcaaa ctggaaaacc tggcgtcgtc atctattctt aaagggcaag gcaactaagc
    10801 ctgcattaat gccaactttt agcgcacggc tctctcccaa gagccatttc cctggaccga
    10861 atacaggaat cgtattcggt ctttttttaa ttgtatttaa aatcaattag ttgcaaacgt
    10921 ctccccgaaa ttccccgaaa tttactcgaa tttctgtatt ccggtctttt ttggttatat
    10981 cacaaccaaa atacatttaa caatccattt acgttaaaat cagagcagta agtacgtttt
    11041 ttctctctca tcaagataca tttttgttgt cttctccgat gtgtggccaa gtagacgctg
    11101 agcaaattct tctccacata tttctttgta caatcgtcca gccaggcttc tgatttcgtg
    11161 aaaagttggt gggttttcac tgaacaggat acctgttaat tttctggctg cgacaaattt
    11221 ctttgtcagg ccgtccgggt gaatgctgcc gtcagggctg ttttttctaa tcccggcact
    11281 gattagataa tcccccctac ttaccatgcg gcactgttca actactgcac caagtcgtaa
    11341 accagcgaca ggaaggctga gtgacagggg gatagcaatc atcattcctg tcttaatttg
    11401 cctgatgtgg agacgatcat cataaatatc actgaaccgc atattcgtta tgtcttcgcg
    11461 acgttgtcct gttacaaggg ctaagtccat agctaatggg aaccatgccg gaagttgatc
    11521 tgctgcctcc ctgatgcagt tgtatgtctt tagtttcagc cgttctcttg taactactat
    11581 tttcggtgct cttgttggcg ttactggatt ttgagatata cgtccttcaa caatggcttc
    11641 gcggaacata tcagataaca cagaacgcat tgatcctgcc atcgtgtttt tccctccttc
    11701 aatctacaaa tcaagaaact cggcgatatg gcgagtggtt atttctgtca gtaaaacccc
    11761 tcctaatttt tcttttattg tctccagttg atttacccga gttttataag tatttctgga
    11821 cgcttttctc cttataagaa tcgttttgta acgttcaatc cagcctgcca tagtaaatga
    11881 gtcgacccct ttaagctttt caattagggc agcaggagag tagtttttgt atatataatg
    11941 atttgcttca attgcctgcg ctactgcatc tcttcttgaa attttaccta gtgtaaattc
    12001 ttctttcgtc agagggttgc gccagtaata tgctttgtct ctccttcgat atgttaagtt
    12061 tctaggcaaa ttgggatcgt attttttccg ctgcatgttt taacttctcc attaacggac
    12121 tgtctctccc ttgtcgccca ttaggctgat ggtgtgttat atcggtatca accttatttg
    12181 ggttgatata gaaagcctcc ggaaccaccc tgtaactcct cccgtgtagt tcaggtgcag
    12241 gataaatgtt tccattcctt gcccatcgtc tcagcgttga tattgatggt gggttatccg
    12301 gatatctgag ttttccccac gttttgagtg tcacaagatt cattgccata cctctcacga
    12361 tatgaccgcc agtaaatata cagaatactg gcgggtgtgg ttgattttta ata
//
LOCUS       DAEBXD010000107        12220 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEBXD010000107 DAEBXD010000000
VERSION     DAEBXD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.107"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..493)
                     /locus_tag="KJL94_003276"
     CDS             complement(<1..493)
                     /locus_tag="KJL94_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4411514.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KJL94_003277"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KJL94_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBD4411515.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KJL94_003278"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KJL94_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD4411516.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="KJL94_003279"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="KJL94_003279"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBD4411517.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="KJL94_003280"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="KJL94_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBD4411518.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="KJL94_003281"
     CDS             3647..4156
                     /locus_tag="KJL94_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4411519.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KJL94_003282"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KJL94_003282"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBD4411520.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="KJL94_003283"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="KJL94_003283"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBD4411521.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="KJL94_003284"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="KJL94_003284"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBD4411522.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="KJL94_003285"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="KJL94_003285"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBD4411523.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="KJL94_003286"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="KJL94_003286"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBD4411524.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="KJL94_003287"
     CDS             complement(8798..9691)
                     /locus_tag="KJL94_003287"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD4411525.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="KJL94_003288"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="KJL94_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="KJL94_003289"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="KJL94_003289"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBD4411526.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="KJL94_003290"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="KJL94_003290"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBD4411527.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2969 a         3155 c         3117 g         2979 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatat ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAEBXD010000108        12154 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEBXD010000108 DAEBXD010000000
VERSION     DAEBXD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.108"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            126..995
                     /locus_tag="KJL94_003291"
     CDS             126..995
                     /locus_tag="KJL94_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBD4411528.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="KJL94_003292"
     CDS             complement(1198..1551)
                     /locus_tag="KJL94_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBD4411529.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KJL94_003293"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KJL94_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBD4411530.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="KJL94_003294"
     CDS             complement(3379..3672)
                     /locus_tag="KJL94_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBD4411531.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="KJL94_003295"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="KJL94_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBD4411532.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KJL94_003296"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KJL94_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBD4411533.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="KJL94_003297"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="KJL94_003297"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBD4411534.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="KJL94_003298"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="KJL94_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBD4411535.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="KJL94_003299"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="KJL94_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBD4411536.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="KJL94_003300"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="KJL94_003300"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBD4411537.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="KJL94_003301"
     CDS             11052..11627
                     /locus_tag="KJL94_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4411538.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="KJL94_003302"
     tRNA            11734..11809
                     /locus_tag="KJL94_003302"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accactaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caat
//
LOCUS       DAEBXD010000109        12144 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEBXD010000109 DAEBXD010000000
VERSION     DAEBXD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.109"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..561
                     /locus_tag="KJL94_003303"
     CDS             <1..561
                     /locus_tag="KJL94_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4411539.1"
                     /translation="LNSLDTRMVPQASAWAINRVAQKAVSVATRQVAGNTVAGDNQVK
                     GIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRG
                     AFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDAR
                     DRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            558..959
                     /locus_tag="KJL94_003304"
     CDS             558..959
                     /locus_tag="KJL94_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4411540.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            970..1710
                     /locus_tag="KJL94_003305"
     CDS             970..1710
                     /locus_tag="KJL94_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBD4411541.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1769..2155
                     /locus_tag="KJL94_003306"
     CDS             1769..2155
                     /locus_tag="KJL94_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBD4411542.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2164..2493
                     /locus_tag="KJL94_003307"
     CDS             2164..2493
                     /locus_tag="KJL94_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBD4411543.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2465..5203
                     /locus_tag="KJL94_003308"
     CDS             2465..5203
                     /locus_tag="KJL94_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD4411544.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5203..5532
                     /locus_tag="KJL94_003309"
     CDS             5203..5532
                     /locus_tag="KJL94_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4411545.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5532..6230
                     /locus_tag="KJL94_003310"
     CDS             5532..6230
                     /locus_tag="KJL94_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBD4411546.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6235..6978
                     /locus_tag="KJL94_003311"
     CDS             6235..6978
                     /locus_tag="KJL94_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4411547.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6915..7517
                     /locus_tag="KJL94_003312"
     CDS             6915..7517
                     /locus_tag="KJL94_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBD4411548.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7578..11058
                     /locus_tag="KJL94_003313"
                     /pseudo
     CDS             7578..11058
                     /locus_tag="KJL94_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            11126..11725
                     /locus_tag="KJL94_003314"
     CDS             11126..11725
                     /locus_tag="KJL94_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBD4411549.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11747..>12144
                     /locus_tag="KJL94_003315"
     CDS             11747..>12144
                     /locus_tag="KJL94_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD4411550.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         2846 a         2873 c         3884 g         2541 t
ORIGIN      
        1 ctgaacagcc ttgatacccg tatggtgcca caggccagcg catgggcgat aaaccgtgtg
       61 gcacagaaag cggtctcggt tgccacccgg caggttgccg ggaataccgt tgcgggcgat
      121 aaccaggtga aagggatccc cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc
      181 agtccgtcag gaaaaatgac ggccaggatc cgcgttaacc ggggcaatct gcccgccatc
      241 aaactgaaca caacacggcg gcgtgctggt gaaggactga gagtgggaaa atactttttc
      301 cggggggcat ttgttcagca actggcgaat ggccgctggc atgttctgag gtgtcttcct
      361 gaagcgcgtt ttgcaacagg gcatgaccat cagggcaggc taagaaaaaa tcgtcttcct
      421 gtggaggtgg tgaaaatccc gatgtccgga ccgctgacac aggcatttga agatgcccgc
      481 gaccgcatca ttgctgcgga aatgccgaaa cagctggggt atgccctgaa acaacaactg
      541 aggttacatc tgagtaaatg aaccgacaca cacaaatccg tcagagcgtg ctggcacgcc
      601 ttcgggaaca gtgtggagac agcgccacgt tttttgacgg gcttccggca tttattgatg
      661 cgcaggaact gcctgccgtg gcggtgtggc tgagtgatgc tcagtacacc ggaaaaatga
      721 cggatgaaga tgactggcag gctgttctgc atattgccgt cttcatccgg gcacaggcac
      781 cggattcaga gctggatatg tggatggaga gcaccatttt cccggccctg aatgatgtac
      841 cggcactttc cggactcatc gacaccctga acccactcgg ttttaactat caacgtgata
      901 atgagatggc tacctgggcg atggcggaaa tcacgtacca gatcacgtac acgaattaag
      961 gaggtggtaa tgaccacacc aaatccactg gcaaagacga aaggtgcggg gacgacgttc
     1021 tggatgtata ccggcaacgg cgatgcgttt gcgaaccctt tgtcggacac tgactggctg
     1081 cgtcttgcga tggtgaagga tctgcaacct ggcgaaatga ccgctgatgc agaagatgac
     1141 acttatctcg atgatgaaga tgcagactgg aaaacgacaa cccaggggca gaaatccgtt
     1201 ggtgatactt cggcgacgct ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt
     1261 cagttgttcg actccggtga agtctgcgcg tttcgtatca aatatcccaa cggcactgtt
     1321 gatgttttcc gcggctggct gagctcactg ggtaaaacca ttgcctcaaa agacgtgatg
     1381 acccgcactg tgaaaatcag cggtgtgggg cgtccgtatc tggcagagga aggcactgaa
     1441 accgtgggtg ttaccggact gacggtggca ccggcatctg tcagtgtcaa agcgggagca
     1501 accaccacac tgacctttac agtaaaacct gacggggcca gtgacaaagc gatcagtgtg
     1561 cattcgtcag atcctcagac tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa
     1621 ggcgtgaagc agggcagtgt cagcatcgtg ggcatgacct ctgacggaga gtttgtggca
     1681 gtggctgcgg tgaccgtcag cgcaccataa caggacgata ctcatcattg ccccggttat
     1741 ccggggcttt ttgcatccgg agaacatgat gtttctgaaa caggatacgt ttaattatga
     1801 aaaacagtcc gtggtgctca gtgagctgtc cgggctgcag agaattgaat atctgacgtt
     1861 tgttcagcag cgaacggcaa agtttgatgc acaggaggga gaactgccgg aggctgaacg
     1921 acagattgct tttctgcgta tgggaatgga tatcaatgcc tggctggttt cccgctcact
     1981 gtggaatgct gagcagtctc aggatgttga gacgctttgc gcatccatta tgacaacatg
     2041 gtcgtatgat gcgctgggcg cgggggcgga gagggttctg tcgctgagcg gtatggggac
     2101 cattgagaat gccggggatg atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg
     2161 gaaatgcagt ttgtcatgcg gcttgcccgg gagttccggc gggcagactg gcggcggatg
     2221 ctgtcggata tgtcggccac tgagcttggt gagtggggcg attatttccg gatgcagagc
     2281 ttcagtgatg tgtggatgga tgcgcagttt gcctcgctga aggcattgat cgtgagaatg
     2341 gtgtccggca gcagcgatgc tgcggtggct gatttcagcc ttttaccgga agagaacggg
     2401 ataccggagc gaacggacga agaactgatg catcttgggg aaggtatttc cggaggtgtg
     2461 cgttatggac cagatagcca acctggtcat tgatttgggg attgatgcgg cagagtttaa
     2521 aaatgaaatt ccccgtatca aaaaccttct gaatggtgca gccagcgatg cagaacggtc
     2581 ttctgcccgt atgcagcgtt ttatggagcg tcagactcag gcggcccggc agacaatgca
     2641 ggcggcttct tcggctgcaa cagcagcatc agcccatgcg cagacggtgg agaagaatgc
     2701 acgggctcat gaacgcatgg cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct
     2761 gaatcagaaa atgcgcgagg aacaggcgca ggccagggcg gctcagctgg gggtaagcag
     2821 tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga aaagccacac attcgctggg
     2881 gctgaaaagt gcggcagccc ggcgggagct gggggtgtta atcagtcaga tggcgcgcgg
     2941 caattttggt gcgctgaggg gatccgggat aacgctggct aaccgtgccg gatggacagg
     3001 cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt attggcggac ttgtcgcggc
     3061 ggtcctgggt ctgggtaaag cctggcatga cggccggaag gagggcgaga aatttaaccg
     3121 tcagctggcg ctgacgggac attatgccgg tgtcactgtc gggcagttgt ggaaactcag
     3181 ccgggccata tccgggaatg gtatcacgca acatgcggca gccggtgcgc tggcacaggt
     3241 ggtgggcagt ggggcatttc atggaaacga tattggtatg gtggcgaaag ctgccgcaca
     3301 gatggagcga tcggttggcc agtcggtcag cgataccata aatcagttta agcggctgaa
     3361 ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg ctgcattttt tgaatgccac
     3421 tcagcttgag cagatacgcg tccttgggga gcaggggcgg tccagtgatg cggcccggat
     3481 cgccatgtct gcgctggcag aggaaaccgg taaacgcaca tctgatattg ataataatct
     3541 caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg tggaagcagt tctgggatgc
     3601 ggccatgaat attggtcgtg aagactcgct ggatgcgcag attgatgcgt tacaggaaaa
     3661 aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc acacaggtgg agtacgatca
     3721 gcagcgtctt aacgatcttc aggagaaaaa acgccggaag gatttgcagg atgcaaaagc
     3781 gcaggcagaa cggaattacc aggagcaaca gaaacgccgg aatgctgaaa atgccgcgct
     3841 gaaccggatg aatgaaacgg aagctgcacg acatcagcgg gaaattgcgc gtattaatgc
     3901 catgcagtac gccgaccagg ctgtcaggga tgcggcgata cagcgtgaaa atgaacgtta
     3961 cgaaaaagcc attaagaaaa atacacgggc aacccgtaat gatgaggcca cccggttatt
     4021 gctgcagtac agtcagcagc aggcacaggt ggaagggcag attgctgctg ccagacagtc
     4081 agcaggcatt gccactgaaa ggatgactga agcgcataaa cagcttctgg ccctgcagca
     4141 gcgcatcagc gacctggacg ggaaaaagct gacggcagat gaaaagagtg tgctggcccg
     4201 taagaatgag ctgattcagg cgctgacgct gctggatgtg aaacagcagg agctgcagaa
     4261 acagacagcg cttaacgacc tgagaaaaaa aacggttcag ctgaccagcc agctggcaga
     4321 caaagaacgt gcactgcgtg agcagcacaa tctggatatt gccactgcag gtatggggga
     4381 taagcagcgg cagcgctacc aggcacagtt gcgcatccgg caggaatacc ggcaacagtt
     4441 gcaacagctt gagaatgaca gtcgccagaa aggcacttac gggacggagg actaccggag
     4501 ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg aatgaaaaca aacgctactg
     4561 gcaggaactg gaagtggcgc agggcgactg gaaaaacggt gccatgcggg cgtttcagaa
     4621 ttttacggcg gatgcggata atgcggcggg aacggcagaa cagatgttta cagtggcatt
     4681 cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc ggtaagctga attttaaatc
     4741 cttcacctct tccctgttgt cagatatggc cagaattatg gcacagatgg ccatgatgca
     4801 ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact aatgctgatg gcggtgttta
     4861 ccagtctgct gatttgagtc gctacagtgg cacggtggtt aaccgtccga cgttttttgc
     4921 ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct gaagccattc tgccactgcg
     4981 tcgcggtgct gatggtaagc tgggggttgt ggcggatact ggtggttcag gcatggtgat
     5041 gtttgccccg cagtacaaca ttgagatcaa taacgacggc acgaacgggc agataggtcc
     5101 ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg gccgcggact ttatgcaaca
     5161 gcaggcccgt gatggtggcc ggttaagtgg agcataccgg taatggagac gtttcactgg
     5221 aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag tggtgacagt gaagctgggc
     5281 gatggttatg aacagcgtcg tgcggcggga ctgaataacc agttgtcgac ttacagcgtg
     5341 acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag cctttctgga acggcacggt
     5401 ggcgtccgcg catttcagtg gacgccacct tatgactgga aactgatcag ggtggtttgt
     5461 cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg taacggccga ttttgaacag
     5521 gtggtgaact gatgcaggat atccggcagg aaacactgaa tgaatgcacc cgtgcggagc
     5581 agtcagcccg ggtggagctc tgggaaatcg atctgacaga ggtcggtggt gagcgttatt
     5641 ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac ctggcagggg cggcagtatc
     5701 aggcataccc cattcagggg tcggggtttg aactgaacgg caggggctgt gctgcccgtc
     5761 cgacactgac ggtctctaac ctgcacggca tggtcaccgg gatggcggaa gatctgcaga
     5821 gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga
     5881 acttcgtcaa cggaaacagc gacgccgatc cggaacagga ggtgatcagc cgctggcgca
     5941 tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt tgtgctggcc acaccaacgg
     6001 agacggatgg cgcggttttc ccggggcgta tcatgctggc gaatacctgt atgtggactt
     6061 accgttctga tgagtgtggt tacacgggca gggcagtggc tgacgagttc gacaaaccaa
     6121 caacggatat ccggaaggac aaatgcagca agtgtatgcg cgggtgtgag ttgcgcaaca
     6181 ataccggtaa tttcggcggt ttcctttcca tcaataaact ttctcagtaa atccatgaca
     6241 cagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgtggc
     6301 ttcgtggtga gaacgccgga gggagacagg tatcttccca gcgagaatat ctccggtgag
     6361 ccggaggaac ggttccggat ggctccggag gactggctgc gggcacaaat gcagggtgag
     6421 attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga ggctgaccgg
     6481 cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc gattcacaag
     6541 ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac ggactgttac
     6601 acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt tcatcgcggg
     6661 gatgactggt ggcgtcacgg tcagaatctc tatctggata atctggaggc cacagggctg
     6721 tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca
     6781 tcggtgccga atcatgccgc catttactgc ggcgatggtg agctgctgca ccatattcct
     6841 gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac acactccctc
     6901 tggcgtcacc gggaatggca cgcatctgcc tttacgggga tttgcaacga tttggccgcc
     6961 gcatcgacct tcgtgtgaaa acgggggctg aagccatccg cgcactggcc acacagctcc
     7021 cggcgtttcg tcagaaactg agtgacggct ggtatcaggt gcgcattgcc gggcgtgatg
     7081 caggtgaaac cgaattgtct gcccgtctta atgagccgct ggcaaatggt gcagtgatcc
     7141 acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt gtttcaggcg gtgctggggg
     7201 cagctgttat ggcggttgct atatggatgc cgggggtagg aattatggcg agtaatctgc
     7261 tgttttctct cggtgccagt atgacgcttg gcggtgttgc acagatgctg gcaccgaaag
     7321 ccagaactcc ccgtacacag acaacggata acggcaaaca gaacacctgt ttctcctcac
     7381 tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa atgcgcgtgg
     7441 ggtcgcgggt ggtatctcag gagatcagca cggcagacga aggggatggt ggtcaggttg
     7501 tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc gcctgcgggc ggttttgtcg
     7561 tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga
     7621 aggacaacct gaaatccacg cagttgctga gtgtgattga tgccatcagc gaagggccgg
     7681 ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa cggtacgccg gtcctggata
     7741 gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt ccgcgccggc gagcaggagc
     7801 agacaccgcc ggaggggttt gaatcttccg gctcagagac tgtgctgggt acggaagtga
     7861 aatacgacac gccgatcacc cggaccatca cgtcggcaaa cattgatcgt ctgcgcctga
     7921 ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag
     7981 tccgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa gacatcacca
     8041 ttaagggcaa aaccacttcg cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc
     8101 gcccgtttaa tatccggatg cgcaggatga caccggacag caccacagac cagctgcaga
     8161 acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca
     8221 cggcgctggt cggcgtgcag gtggattcag agcagttcgg taaccagcag gtgagtcgca
     8281 attatcatct tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg cagacgcggc
     8341 aatacagcgg tatctgggac ggaacgctta agccagcata cagcaacaac atggcctggt
     8401 gtctgtggga catgctcact catccgcgct acggcatggg gaaacgtctt ggtgcggcag
     8461 atgtggacaa atgggcgctg tatgtcatcg gccagtactg cgaccagtca gtgccggacg
     8521 gatttggcgg cacggagccg cgcatcacct gtaacgctta cctgaccaca cagcgtaagg
     8581 cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacgggc
     8641 agacgctgac gttcgtgcag gaccgaccgt cggataaagt gtggacctat aaccgcagta
     8701 atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc
     8761 gccataatgt cgttgaggtg aactggattg atccggacaa cggtcatgag acggcgacag
     8821 agcttgtgga ggacacgcag gccattgtcc gttacggtcg taacgtcacg aagatggatg
     8881 cctttggctg taccagccgg ggacaggcgc accgcgccgg gctgtggctg attaaaacgg
     8941 aactgctgga aacgcagacc gtggacttca gcgtcggcgc agaagggctt cgccatgtac
     9001 cgggtgatgt cattgaaatc tgcgatgatg attatgccgg tatcagcacc ggtggtcgcg
     9061 tactggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc acgctgccgt
     9121 cctccggtac cacgctgata agcctggttg acggaaatgg caatccggtc agcgtggagg
     9181 ttcagtccgt caccgacggc gtgaaggtga aagtgagccg ggttcctgac ggcgttgcag
     9241 aatacagcgt gtgggggctg aagctgccga cgctgcgcca gcgcctgttc cgctgcgtga
     9301 gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc cgtgcagcat gtgccggaaa
     9361 aagaggccat cgtggataac ggggcacact ttgacggtga ccggcgcggc acggtgaatg
     9421 gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt caccgcagac agcggggaat
     9481 atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa gggtgtgagc ttcctgcttc
     9541 gcctgaccgt ggcagcggac gacggcagtg agcggctggt cagcacagcc aggacgacgg
     9601 aaaccacata ccgcttcagg cagctggcgc tggggaatta cagtctgaca gtccgggcgg
     9661 taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt gcggcaccgg
     9721 cagcgcctgt cactattgaa ctgataccgg ggtattttca gataacggtg gtcccgaaac
     9781 tggctgtata tgacccgacg gtgcagtttg agttctggtt ttcggaaaag cggattgcgg
     9841 atatcaggca ggttgaaacc agcgcccgct atcttggcac ggcgctgtac tggatagccg
     9901 ccagtatcaa tatcaggccg ggccatgatt attattttta cgttcgcagt gtgaacaccg
     9961 ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag cgatgatgcg gaaggttatc
    10021 tgagttttta taaagggttg atcaataaaa cgcatctcgg caaggagttg tggacgcaga
    10081 ttgataacgg tcagcttgcg ccggacctga ctgaaatcag gacgtccatt acgaatgtca
    10141 gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa tcagagtgct gcaatccagc
    10201 agatacagaa agttcaggtt gatacaaata ataacctgaa cagcatgtgg gctgtgaagc
    10261 tgcaacagat gaaggacgga cgcctttata ttgcgtggta tcggtgccgg tattgagaat
    10321 acgccagcag gaatgcagag tcaggtgctg ctggcggcag acaggattgc gatgattaat
    10381 cctgcgaatg gcaacacaaa gccgatgttt gttggtcagg gcgatcagat attcatgaac
    10441 gacgtgttcc tgaaacgcct gacggctccg accattacca gcggcggtaa tcctccggca
    10501 ttttccctga cacctggcgg acggctgacg gcgaaaaatg ccgatatcag cggtaacgtg
    10561 aacgcgaact ccgggacgct caacaacgtc acgattaata agaactgtcg ggttctggga
    10621 aaattgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa acctttccca
    10681 cgggactccc gggcaccgga gaggtggcca tcagggacca ttaccgtcag ggtttatgac
    10741 gatcagccgt ttgatcggca aattgttatt cccgcggtgg cgtttcgcgg tgctaaacat
    10801 gagcggaaga ataacaatat ttattcgtca tgccgcctga tagtgaagaa aaacggtgct
    10861 gaaatttata accgaacgac cctggataat acgctgatat atacgggtgt tattgatatg
    10921 cctgccggtc acggtcacat gacgctggag ttttctgtat cggcatggct ggtaaatggc
    10981 tggtatccca cagcaagtat cagcgatttg ctggttgttg tgatgaagaa atccacagca
    11041 ggtatcagta tcagctgaat tttataaccc agatacgggc accagaaatg gtgccttttt
    11101 tattgcagaa aagcgagagg taattatgcg taaactttat gctgccattt tgtccgcagc
    11161 catctgtctg gctgtatccg gtgcgcctgc atgggcgtct gaacatcagt ccacgctgag
    11221 cgcggggtat cttcatgcct cgacgaacgc tcccggcagt gatgatctga acgggattaa
    11281 cgtgaaatac cgttatgagt ttacggacac gctggggctg gtgacgtcat tcagctatgc
    11341 caacgctgaa gatgagcaaa aaacgcatta cagtgatacc cgctggcatg aggattccgt
    11401 gcgtaaccgc tggttcagcg tgatggcggg gctgtctgtg cgcgtgaatg aatggttcag
    11461 cgcgtatgcg atggcgggcg tggcttacag ccgtgtgtcg accttctccg gggattatct
    11521 ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg accgggagtg atgacaaccg
    11581 ccacagcaac acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt
    11641 ggccattgac cttgcttatg aaggttccgg cagtggcgac tggcgcactg acggtttcat
    11701 cgtgggtgtc ggttataagt tctgattagc caggtaacac agtgttatga cagcccgctg
    11761 gttcaggcgg gctttttgtg gggtgaatat ggcagtaaag atttcaggtg tactgaaaga
    11821 tggggcgggt aaacctgtcg taaattgtgc gattgaactg cgggccagaa gaaccagtcc
    11881 gaccgttgtg gcacacgttg ttgccacttg cgtgacggac aataacggtg cttatgtgat
    11941 tgaggctgag ccggggtatt acgaggttgc gcttcactgt aacggctggc agccaacccg
    12001 tgtcggggat attgatgtgg caccgactga tgcaccgggg acactgaacg cgtttctgaa
    12061 tgcaccaaag gatggtgatt tacgtccgga ggtgatgaag cgctttgagg aaatggtggc
    12121 gcaggcgcag cagagtgccg gggc
//
LOCUS       DAEBXD010000110        11921 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEBXD010000110 DAEBXD010000000
VERSION     DAEBXD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.110"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..2338)
                     /locus_tag="KJL94_003316"
     CDS             complement(<1..2338)
                     /locus_tag="KJL94_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD4411551.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDHTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="KJL94_003317"
     CDS             complement(2272..2895)
                     /locus_tag="KJL94_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBD4411552.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="KJL94_003318"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="KJL94_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="KJL94_003319"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="KJL94_003319"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBD4411553.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDEKVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="KJL94_003320"
     CDS             7503..8225
                     /locus_tag="KJL94_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBD4411554.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="KJL94_003321"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="KJL94_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBD4411555.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="KJL94_003322"
     CDS             complement(10297..11892)
                     /locus_tag="KJL94_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBD4411556.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2758 a         3306 c         2944 g         2913 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgtggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccgga tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 tttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAEBXD010000111        11669 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEBXD010000111 DAEBXD010000000
VERSION     DAEBXD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.111"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            597..794
                     /locus_tag="KJL94_003323"
     CDS             597..794
                     /locus_tag="KJL94_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411557.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KJL94_003324"
     CDS             complement(744..1937)
                     /locus_tag="KJL94_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411558.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KJL94_003325"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KJL94_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBD4411559.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KJL94_003326"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KJL94_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBD4411560.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KJL94_003327"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KJL94_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBD4411561.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KJL94_003328"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KJL94_003328"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBD4411562.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KJL94_003329"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KJL94_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBD4411563.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="KJL94_003330"
     CDS             complement(10573..10716)
                     /locus_tag="KJL94_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411564.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11669
                     /locus_tag="KJL94_003331"
     CDS             10966..>11669
                     /locus_tag="KJL94_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4411565.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2961 a         2900 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggactc
//
LOCUS       DAEBXD010000112        11601 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEBXD010000112 DAEBXD010000000
VERSION     DAEBXD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.112"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(282..357)
                     /locus_tag="KJL94_003332"
     tRNA            complement(282..357)
                     /locus_tag="KJL94_003332"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3138
                     /locus_tag="KJL94_003333"
                     /pseudo
     CDS             916..3138
                     /locus_tag="KJL94_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3474..4451
                     /gene="csiD"
                     /locus_tag="KJL94_003334"
     CDS             3474..4451
                     /gene="csiD"
                     /locus_tag="KJL94_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBD4411566.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4471..5739
                     /gene="lhgO"
                     /locus_tag="KJL94_003335"
     CDS             4471..5739
                     /gene="lhgO"
                     /locus_tag="KJL94_003335"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBD4411567.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5762..7210
                     /gene="gabD"
                     /locus_tag="KJL94_003336"
                     /pseudo
     CDS             5762..7210
                     /gene="gabD"
                     /locus_tag="KJL94_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7218..8504
                     /gene="gabT"
                     /locus_tag="KJL94_003337"
     CDS             7218..8504
                     /gene="gabT"
                     /locus_tag="KJL94_003337"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBD4411568.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8742..10142
                     /gene="gabP"
                     /locus_tag="KJL94_003338"
     CDS             8742..10142
                     /gene="gabP"
                     /locus_tag="KJL94_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBD4411569.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10163..10825
                     /gene="csiR"
                     /locus_tag="KJL94_003339"
     CDS             10163..10825
                     /gene="csiR"
                     /locus_tag="KJL94_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBD4411570.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10826..11275)
                     /gene="kbp"
                     /locus_tag="KJL94_003340"
     CDS             complement(10826..11275)
                     /gene="kbp"
                     /locus_tag="KJL94_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBD4411571.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11359..11517)
                     /locus_tag="KJL94_003341"
     CDS             complement(11359..11517)
                     /locus_tag="KJL94_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBD4411572.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2984 a         2848 c         2894 g         2875 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtagctc
     2761 tacaatgaat tccacgcatt atatatagat aaagcaggaa tatggagcgc atttgagggt
     2821 gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg attctgtgaa cattaatcac
     2881 tctcctcatg atggactagt tataataaat aaaggaaacg aagaagttga aggtacctgg
     2941 cctaacaaat tgcaacctgg aatatataaa aacatgggga gtaatagcgt taacattatt
     3001 attaataata cccgaaaaat tatcccccct tgtaaagtat ttacgcttag aggcggatct
     3061 ctaaatatca atattcctag gcgtagcgct cttcttttag ggaaaacagg agaaccgccg
     3121 aactatctct atttataatt taatgttata tctgccccga taaaacgggg cagataatat
     3181 gtttagttta ctaacggtca ttttgtagtg aagccattta ctgtttttta tcgaccagat
     3241 aatctgttct ctaatgttaa ctccccctaa cctgttgctt tagttattca tttcctgtct
     3301 cactttgcct taatacccta cgttaaatgt tactaatttg ttgcttttga tcacaataag
     3361 aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt agatattttt agattatggc
     3421 tacgaaatga gcatcgccat gtcaccctac atctcataag aggatcgctt ctgatgaatg
     3481 cactgaccgc cgtacaaaat aacgctgtcg attcaggcca ggactatagc ggattcactc
     3541 tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac cttcaccgaa cagacgacca
     3601 aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc gctggagtac aaatcgtttc
     3661 tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc gaatcagctg caaccattgc
     3721 tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt gatcaatgcg gtaggtatcg
     3781 atgatgtggc gcaggcagat gagatggtga agctggcaac ggcggtagcg catctgattg
     3841 gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc gcgtttcgtg gtgaaaaatg
     3901 tcgataactc agacagctat ctgcgtcagc cgcaccgcgt gatggaactg cacaacgacg
     3961 gcacgtatgt cgaagagatc accgattacg tgctgatgat gaaaatcgat gagcaaaaca
     4021 tgcagggtgg aaattcgttg ctgctgcatc tcgatgactg ggaacatctg gaccactatt
     4081 tccgccatcc actggcgcgt cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca
     4141 gcaaagatgt tttccatcca gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct
     4201 atatcgacca gttcgtccag ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt
     4261 ccgatgccat tgaaaccagc aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc
     4321 tgttgataaa caacctgttc tggctgcacg gtcgcgaccg ctttactccg cacccggatc
     4381 tgcgccgtga actgatgcgt cagcgtggct atttcgctta cgccactcac cactaccaga
     4441 cgcatcagta agcgcaaagg aattgagcgg atgtatgatt ttgtgattat tggcggcggc
     4501 atcatcggca tgtcgaccgc catgcaactg attgatgttt atccggatgc ccgcattgcg
     4561 ttgctggaaa aagagtccgg cccggcctgt caccagacgg gccacaacag cggcgtgatc
     4621 catgccgggg tctattacac gcccggcagc ctgaaggcgc agttttgcct ggcggggaac
     4681 cgtgccacca aagccttttg cgatcaaaac ggcattcgct atgacaactg cggcaagatg
     4741 ctggtcgcca cgtctcagct cgaaatggaa cggatgcgcg ccttatggga acgcacagcg
     4801 gcgaacggta tcgagcgcga gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat
     4861 atcaccgggc ttggtggcat ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg
     4921 acggcggcaa tggcaaaaat cttccaggcc agaggcggcg agattattta taacgccgaa
     4981 gtcagcgccc tcagtgagca taaaaacggc gtggtgatac gcacccgtca gggcggtgaa
     5041 tatgaagcat cgacgctgat tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg
     5101 ctcggcctcg aaccgggctt tattatctgc ccgttccgtg gcgagtattt ccgccttgcg
     5161 ccggagcata accagattgt taaccacctg atttacccca ttcccgaccc cgcgatgcca
     5221 tttttgggcg tgcatctcac ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg
     5281 gtgctggctt tcaaacgcga aggctatcgc aagcgcgact tctcatttag tgacacgctg
     5341 gagattttga gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg
     5401 ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt
     5461 ccccggcttt cgttaagcga tctccagccc tggcccgccg gtgtgcgggc gcaggcagta
     5521 tcgccggacg gcaagctgat tgacgatttt ctgtttgtca ccaccccacg cacgatccac
     5581 acctgcaatg cgccctcccc ggcagcgaca tcagcaattc ctattggtgc gcatattgtc
     5641 agcaaggtac aaacgctgtt ggcaagccag agtaaccccg gacgcacgct gcgagcggca
     5701 cgtagtgtgg atgccttaca cgccgcattt aatcaataac ctttgaaaac aggacgtaac
     5761 aatgaaactt aacgacagta acttattccg ccagcaggcg ttgattaacg gggaatggct
     5821 ggacgccaac aatggtgaag tcatcgacgt caccaatccg gcgaacggcg acaagctggg
     5881 tagcgtgccg aaaatgggcg cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc
     5941 cctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc accattctgc gcaactggtt
     6001 caatttgatg atgcagcatc aggacgattt agcgcgcctg atgaccctcg aacagggtaa
     6061 accgctggct gaagcgaaag gcgaaatcag ctacgccgcc tccttcattg agtggtttgc
     6121 tgaagaaggc aaacgtattt atggctacac cattcctggt catcaggccg ataagcgcct
     6181 gattgttatc aagcagccga ttggcgtcac cgccgccatc acgccgtgga actttccggc
     6241 ggcgatgatt acccgtaaag ccggtccggc gctggcggca ggctgcacca tggtgctgaa
     6301 gcccgccagt cagacgccgt tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg
     6361 cattccggct ggcgtgttta acgtggtcac cggttcggca ggcgcggtcg gtaatgaact
     6421 gaccagcaac ccgctggtac gcaaactgtc gtttaccggt tagaccgaga ttggccgcca
     6481 gttaatggaa cagtgcgcga aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc
     6541 gccgtttatc gtctttgacg atgccgatct cgataaagcc gtggaaggcg cgctggcctc
     6601 gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg
     6661 cgtgtatgac cgctttgccg aaaaattaca gcaggcggtg agcaaactgc acatcggcga
     6721 cgggctggag aaaggcgtca ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt
     6781 ggaagagcat attgccgatg cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc
     6841 gcacgaacgc ggcggcaact tcttccagcc aaccattctg gtggacgttc cggccaacgc
     6901 caaagtgtcg aaagaagaga cgttcggccc cctcgcccca ctgttccgtt ttaaagatga
     6961 agccgatgtg attgcacaag ccaatgacac cgagtttggt cttgccgcct atttctacgc
     7021 ccgtgattta agccgcgtct tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat
     7081 caataccggc attatttcca atgaagtggc cccattcggc ggcatcaaag cctcgggtct
     7141 gggtcgtgaa ggttcgaagt atggcatcga agattactta gaaatcaaat atatgtgcat
     7201 cggtctttaa ctggagaatg cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc
     7261 aggcgattcc ccgtggcgtt gggcaaattc acccgatttt cgctgaccgc gcggaaaact
     7321 gccgggtgtg ggacgttgaa ggccgtgagt atcttgattt cgcgggcggg attgcggtgc
     7381 tcaataccgg gcacctgcat ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac
     7441 tgtcgcacac ctgcttccag gtgctggctt acgagccgta tctggagctg tgcgagatta
     7501 tgaatcagaa ggtgccgggc gatttcgcca agaaaacgct gctggttacg accggttccg
     7561 aagcggtgga aaacgcggta aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg
     7621 cttttagcgg cgagtatcac gggcgcacgc attacaccct ggcgctgacc ggcaaggtga
     7681 atccgtactc tgcgggcatg gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt
     7741 gcccgctgca cggcataagc gaggatgacg ctatcgccag catccaccgg atcttcaaaa
     7801 atgatgccgc gccggaagat atcgccgcca tcatgattga gccggttcag ggcgaaggcg
     7861 gtttctacgc cgcgtcgcca gcctttatgc agcgtttacg cgctctgtgt gacgagcacg
     7921 ggatcatgct gattgccgat gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg
     7981 cgatggagca gatgggcgtg gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg
     8041 gtttcccgct ggcgggcgtc accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg
     8101 gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga
     8161 aggtgttcga gcaggaaaat ctgctgcaaa aagccaacga tctggggcag aagctaaaag
     8221 acggattgtt ggcgatagcc gaaaaacacc cggagatcgg cgacgtacgc gggctgggat
     8281 cgatgattgc catcgagctg tttgaagatg gcgatcacag caaaccggac gcaaaactca
     8341 ccgccgagat cgtggcacgc gcccgcgata agggcctgat tcttctctcc tgcggcccgt
     8401 attacaacgt gctgcgcatc cttgtaccac tcaccattga agacgctcag atccgtcagg
     8461 gtctggagat tatcggtcag tgttttgatg aggcgaagca gtagcaccgc ccccatgcag
     8521 gaggcgacgc tgcgcgtctt gtccggccta cgagaatcca agtcggataa ggcgtttacg
     8581 ccgcatccga caatctgtac gtgaacagga ataaatctat gtcggccgga taaggcggag
     8641 ccgctctccg gcaaaaagaa tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc
     8701 ggggcgctct cccaagtgac acactttcga gaggattcag gatggggcaa tcatcgcaac
     8761 cacacgagtt aggcggcggg ctgaaatcac gccacgtcac catgttgtct attgccggtg
     8821 ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg
     8881 tattactggc ctatctgttc gccggattac tggtggttat gattatgcgg atgctggcgg
     8941 aaatggcagt tgctacgccc gataccggtt cgttttccac ctatgccgat aaagccattg
     9001 gccgctgggc gggctatacc atcggctggt tgtactggtg gttttgggta ctggttatcc
     9061 cactggaagc caacatcgcc gccatgatcc tgcactcgtg ggttccaggc attcccatct
     9121 ggttattttc cctcgtcatt accctcgcct taactggcag taatttatta agcgttaaaa
     9181 actacggcga atttgagttc tggctggcgc tgtgcaaagt catcgctatc ctggccttta
     9241 ttttccttgg tgcagtcgca attagcggtt tttaccctta tgccgaagtg agcgggatct
     9301 caagattgtg ggatagcggc ggctttatgc ccaacggttt cggtgcggta ttaagcgcga
     9361 tgttgatcac catgttctcg tttatgggcg cagaaattgt caccattgcc gccgcggaat
     9421 ccgacatgcc ggaaaaacat attgtccgcg ccaccaactc ggttatctgg cgtatttcta
     9481 tcttctattt gtgctctatt tttgtcgtcg tggcattaat accgtggaat atgccggggc
     9541 tgaaagccgt tggatcttat cgctcggttc tggaattgct caatattccc catgcgaaat
     9601 taatcatgga ctgtgtgata ttactttccg taaccagttg cctgaactcg gccctgtata
     9661 ccgcctcaag gatgctctac tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta
     9721 aaatcaaccg cagtaaaacc ccgtacgtgg cggtgttact ctccaccggc gcagcattct
     9781 taacggtggt ggtgaactat tacgcgcctg cgaaggtatt taaatttctg atcgacagct
     9841 ccggcgctat cgccctgctg gtttatttag tcatcgccgt ttcacagttg cggatgcgca
     9901 aaattctgcg agcagaagga agcgaaattc gcttgcgaat gtggcttgat ccctggctca
     9961 cctggctggt catcagcttt attacctttg tgttggtagt gatgctattc cgtccggcgc
    10021 aacagttaga agtgatctcc accggcttat tagcgatagg gattatctgt accgtgccaa
    10081 ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat aatacgcgct
    10141 gataatgatc aggagtcaca ccatgaccat tacgtctctg gatggctatc gctggctgaa
    10201 gaacgatatt attcgcggta attttcaacc ggatgaaaaa ttacggatga gtttactgac
    10261 atcgcgttat gcacttggcg ttgggccgtt gcaggaagca ctttctcaac tggtggcgga
    10321 acggctggtc acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga
    10381 gctgctcgat attttcgacg cccgcgccaa tatggaagcg atgctggtca gtctggcgat
    10441 tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa
    10501 gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc
    10561 gtttcataca gcaattgtgg cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg
    10621 gttgtttgat ctggcggcgc gttatcgatt tatctggctg cggcgaacgg tgctttcggt
    10681 ggaaatgctg gaggataaac acgatcagca ccagaccctg actgcggcgg tactgccgcg
    10741 agatacagcg cgcgccagtg agttaatgcg ccagcattta ctgacgccaa ttcccattat
    10801 ccagcaggcg atggctggca attaactact cttccggaat acgcaacact tgccccggat
    10861 aaattttatc cgggcttttt agcatcggtt tattcgcttc gaagatttta ttgtacagat
    10921 tagcgttacc gtagacctgt ttggaaatgg cactcagagt gtcgccagac ttaacggtat
    10981 aaaactggct ggcagtggct ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac
    11041 cggaaatatt ccccaccgca acaaggattt tctccttcgc ctcctgactc aggccgtcac
    11101 cagtgaccgt cgctttgccg tcggcaattt gaatgttcac tttatcggca tcaggtatac
    11161 cggttttgct cagatgctcc tgcaccttct tcgcctgatc gtctttatcg tgctgacctg
    11221 taaccgcatc ccagagtttt tctccggcat ctttaacaaa attgaacaga cccatagcta
    11281 cctcattcgt taacggaaac actcagaaag tgtagcagag gaacgcggcg cgttaacgcc
    11341 gcgggggtga gcacaggttt aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg
    11401 gatataccca agcagtgtca gcagaatatt aataatgaac gcccaaccga accctttacc
    11461 gagcagcacg ccgatcggcg gcagaatgat ggtgatgacg attctccaga aacccatatg
    11521 tactccctat aagaaaatta ctcattgttt aaaaagagat tttatctctt aagcgtagta
    11581 attttagcgg agcctgccag t
//
LOCUS       DAEBXD010000113        11344 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEBXD010000113 DAEBXD010000000
VERSION     DAEBXD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.113"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..658)
                     /locus_tag="KJL94_003342"
     CDS             complement(<1..658)
                     /locus_tag="KJL94_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD4411573.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     D"
     gene            complement(735..1039)
                     /locus_tag="KJL94_003343"
                     /pseudo
     CDS             complement(735..1039)
                     /locus_tag="KJL94_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1043..1945)
                     /locus_tag="KJL94_003344"
     CDS             complement(1043..1945)
                     /locus_tag="KJL94_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBD4411574.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(2812..3783)
                     /locus_tag="KJL94_003345"
     CDS             complement(2812..3783)
                     /locus_tag="KJL94_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HBD4411575.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(3783..4949)
                     /gene="sopA"
                     /locus_tag="KJL94_003346"
     CDS             complement(3783..4949)
                     /gene="sopA"
                     /locus_tag="KJL94_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HBD4411576.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(5537..6292)
                     /gene="repE"
                     /locus_tag="KJL94_003347"
     CDS             complement(5537..6292)
                     /gene="repE"
                     /locus_tag="KJL94_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HBD4411577.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(7066..7872)
                     /locus_tag="KJL94_003348"
     CDS             complement(7066..7872)
                     /locus_tag="KJL94_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBD4411578.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(7873..8178)
                     /gene="ccdB"
                     /locus_tag="KJL94_003349"
     CDS             complement(7873..8178)
                     /gene="ccdB"
                     /locus_tag="KJL94_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBD4411579.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(8180..8398)
                     /gene="ccdA"
                     /locus_tag="KJL94_003350"
     CDS             complement(8180..8398)
                     /gene="ccdA"
                     /locus_tag="KJL94_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBD4411580.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            9106..10101
                     /locus_tag="KJL94_003351"
     CDS             9106..10101
                     /locus_tag="KJL94_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBD4411581.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            10105..11037
                     /locus_tag="KJL94_003352"
     CDS             10105..11037
                     /locus_tag="KJL94_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411582.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
     gene            complement(11174..>11344)
                     /locus_tag="KJL94_003353"
     CDS             complement(11174..>11344)
                     /locus_tag="KJL94_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4411583.1"
                     /translation="GKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDK
                     VIGHYLNIKHYQ"
BASE COUNT         2984 a         2914 c         2710 g         2736 t
ORIGIN      
        1 catcattcgc ggccccctgc tgcatggccc gttgcacggc ggcaaggcgt tgctgcccgg
       61 cacggtgata ctgctcaagc agctcgatac cgatataccg gcgtccggac tggagggcgg
      121 cgacgcaggt tgagccgctg cctgcaaacg ggtccagcac aattgcgttc gggtgtgtga
      181 agctctcaat cagcggttgc aggctggtaa cgggcttttc cgtcgggtga tgacgattgc
      241 cagaatattt ccagcccagc acgtccggca gcgggttttg tggcagacgt ggacggcctt
      301 ttgccaggat gtaggcgcat tcgtggcgat agcccacata tgcggccttc gatgtgtagt
      361 ttttggtgaa gaccaggtga ccaacaacgc tgaatcccgc atttttccag gcggccataa
      421 agcgatcgac gcggttccag ccgtagaagc tcaccatcag cgcgtctttt ttgagtacgc
      481 ggtacatttc attacaggcc ggttgcagcc attcatcggt tttatcgccg gcgatggtgc
      541 gcccggaacg gtcacgaaaa ccgacgagat atggcgggtc agttaatatg aaatcaatgg
      601 cgttatcagg gatacgcgtc ataacatcga tgcagttacc aaggacaaaa cgggacataa
      661 caacgctcct tctcttacag agggcaccgg gagggccccc ctgtggtgcc cgttccccgc
      721 tgcctgtggg ctgttcaccg cgtccggtaa cgggtcaggc cgaccgtgga atatcgcagc
      781 cgtcgtcttt ttgacggcga gaatatgccg cgaaagcggc ttgaagggcg ttcatcagaa
      841 aagggcgaca ggctggcgta cactgtcacc ggtgccagcc gggagcagta aaagcgcccg
      901 tcccgtgccc gccggacgcg ggacactgac cacaggcagc gttcctgccg cgccgcgtca
      961 gccggcgcag taaaaagagg agcacggcga catcctttgt cggaaaagaa acgggcggtt
     1021 gcccgcccgt ctccgtcata cttcatcccc cgtaatgcgc caccttgtcg actatccact
     1081 ccggcgcggt gacttccggc agctcatcat catctgtcgg ggaagaccat tccagttccc
     1141 cccacagcac atcaacaagc tctccacgct catatcgctg ccagccacag aaattgcagc
     1201 cctgttcggc gtaccagtgc tccagcgtgc agccgaaacg gcggcttaat tctgcaacaa
     1261 cgttgctttc cggctgacac cacggcgtgt cgaaatccac ttgaatgaag ttttctccct
     1321 gacggctgat attcacctca taccccacag gccacttcac gccatactgt tccgtatacc
     1381 agtgatacgc agaaggaaca ccgttcagaa caccaccgtt aaagccattc acttcaacat
     1441 ccaggcgggt gggcagaacc gtcagcaggt caaacggcat ggttttttcc ggcagtacat
     1501 tgtcacacag acggttccac cacaccgata catcctcgtt actccagaag ccacaccagt
     1561 cacctctttt tgcggtgaaa tgcacggtga tggtttccct gacctcatcc ggtaatcctt
     1621 cccagcgacg ctggccagta ccactctgca gccagagttc atgcagcatc aggcagtttt
     1681 gctcatccag cagtacaccg ttctgcaggt gtgtcagcca gcgggtgaac gcgatattct
     1741 ccggcgatac aacaccacgt ccggcatccg tcagtccggg gcacggttca aactgcacat
     1801 cttcagtggt ctgcagaagt ccggcacttc cggccagaaa cagctgaata ccttcatttg
     1861 tggcgcggcg ataaagcggt gtgacggcac cgctggccag tcgtttaatc tcagcaatct
     1921 gtgccggttc accagaaaaa tgcatacgat tcgagcacca gtttggcatg ggaattctcc
     1981 tctcacatgc ggcctccatc aggccgtgcc gcacctgtaa ccgggcgacc gcatacgaat
     2041 gcaggtgcgg ccccgaagcc gcagcgcgtg gaggaggaac gacgaaacgt gaccgggtga
     2101 ggaggccggc gacagcgaca cacttgcatc ggatgcagcc cggttaacgt gccggcacgg
     2161 cctgggtaac caggtatttt gtccacataa ccgtgcgcaa aatgttgtgg ataagcagga
     2221 cacagcagca atccacagca ggcatacaac cgcacaccga ggttactccg ttctacaggt
     2281 tacgacgaca tgtcaatact tgcccttgac aggcattgat ggaatcgtag tctcacgctg
     2341 atagtctgat cgacaataca agtgggaccg tggtcccaga ccgataatca gaccgacaac
     2401 acgagtggga tcgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt
     2461 cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag
     2521 accgacgata cgagtgggac catggtccca gactaataat cagaccgacg atacgagtgg
     2581 gaccatggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
     2641 aataatcaga ccgacgatac gagtgggacc gtggtcccag tctgattatc agaccgacga
     2701 tacaagtgga acagtgggcc cagagagaat attcaggcca gttatgcttt ctggcctgta
     2761 acaaaggaca ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc
     2821 tggcttttca agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg
     2881 agacctgtcc aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc
     2941 aaactgatgt cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt
     3001 taaaagagtg ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag
     3061 gttagatgcc tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc
     3121 acctgaccgg gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa
     3181 tttggctgtg ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag
     3241 cgcagaaata tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc
     3301 ataagcactt gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc
     3361 atccagctcg ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg
     3421 gcgactccca tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg
     3481 ttgaccagtc agtagaaaag aagggatgag atcatccagt gagtcctcag taagcagctc
     3541 ctggtcacgt tcattacctg accatacccg agaggtcttc tcaacactat caccccggag
     3601 cacttcaaga gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg
     3661 agccattact cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa
     3721 cgaagtatct ccaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt
     3781 cattatctaa tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt
     3841 tcccaaatag aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt
     3901 tcaaaaacag ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca
     3961 ttttttagaa ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga
     4021 gagccattac tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta
     4081 agatcaacgt tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag
     4141 tcaaacaact cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc
     4201 gtgccgatac ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca
     4261 gtttcaatgg ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat
     4321 ttgcccatta actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc
     4381 ccaggccagc aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa
     4441 ggcaggagag tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag
     4501 gctgttccct gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga
     4561 tcctgagcaa gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc
     4621 ccgatcaccg gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc
     4681 atatgattaa tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc
     4741 atatccgggt gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc
     4801 ccaactaaat ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg
     4861 ctgtcatcat taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg
     4921 cactggttaa gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt
     4981 tttttattaa atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa
     5041 accgcaaagt tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc
     5101 tcagtcactt attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac
     5161 tggcgaggaa gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga
     5221 aatatccacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt
     5281 aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca
     5341 catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc
     5401 ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa
     5461 ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg
     5521 acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga
     5581 tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc
     5641 tgttgatctc attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt
     5701 aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg
     5761 agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg
     5821 tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga
     5881 agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac
     5941 gtttgataaa ccagggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc
     6001 gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg
     6061 cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac
     6121 cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca
     6181 tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga
     6241 ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc
     6301 catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag
     6361 ggataacttt gtgaaaaaac agcttcttct gagggcaatc tgtcacaggg ttaagggcaa
     6421 tttgtcacag acaggactgt catttgaggg taatttgtca cactgaaagg gcaatttgtc
     6481 acaacacctt ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga
     6541 tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca
     6601 agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct
     6661 tcctcgctca ctcgagggct tcgccctgtc gctcgactgc ggcgagcact actggctgta
     6721 aaaggacaga ccacatcatg gtgctgtgtt cattaggttg ttctgtccat tgctgacata
     6781 atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc
     6841 aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta
     6901 cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt
     6961 ttcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata
     7021 tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg ataattgttt
     7081 cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac
     7141 atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat gtcccatcag
     7201 gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat aggaatggcg
     7261 gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac
     7321 cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc
     7381 tgtccttcct gtgcgacggt tacgccgctc catgggtatt ttcagtgttg ccaccatcgt
     7441 ctgcagctgg ctgacgtacc aggagtcaga gagcggaacc agccggtgag tctgctgacc
     7501 ggcgggcatt ctccccgccg tcctggcagc tttttcggtc cgttgtttca gggtcgcaag
     7561 ctgcacaaac ggatacggag gcgcaagcga aaaatccccc cgcgtcagcg ccagtgcttc
     7621 attaatgcgt gctccggtgt tccacagtgt ggccagcagc atcttgcggt gcagatccgg
     7681 gacgtaatgg agcagggcac tcacttccgg agccagcaga tattttggca gttcatcatg
     7741 gaccatcgac atctggcgaa gtgccagagc tgccggataa tcaatggcaa caggcagcga
     7801 tgcaggctgc ccggcagaat acactgccgg taccatgact gcagactggc tgtgtataac
     7861 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
     7921 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
     7981 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
     8041 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
     8101 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
     8161 gtaaacctta aactgcattt caccagtccc tgttctcgtc agcaaaagag ccgttcattt
     8221 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
     8281 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat
     8341 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
     8401 cacacctctt tttgacatac ttcgggtata catatcagta tatattctta tgccgcaaaa
     8461 atcagcgcgc aaatacgcat actgctatct ggcttttagt aagccttatg tattttacct
     8521 ttcgttatgt taaccaataa aaattaaaat ctgacttata aaaacaaagc gtaattaccg
     8581 cattcccgtt tcgtatggat ccgactttat ttgaataaac gactttccgg aagtgtacaa
     8641 cgctgagaaa atcatcatat cgctgttttc cgcccctgcg cggcgcatca cagtccagag
     8701 atacaacaca acatatagtg ccatgggcaa ctgtatgagg cactacatgc tgtgctgacg
     8761 ggatgtaacg ctgtgcggag ggagaggaag atccgtaact tacttttttc ggatctctgg
     8821 agaggggaag aaggatttgt gctgatgatg caatactaat gatatatttt gaacttaatt
     8881 gcattattgc ttatctggca ttaataactg aaatactttg cataattatc aatgaaagaa
     8941 cgaggctcat atttcccatg taaattatat gcccttgcag agagcgagac ggtagtgaac
     9001 ttgcggcact aataataaac tgtaccgact cttcccgggc actggaatgt tcagatgtac
     9061 gacagagccc cgcgcctttt gcaataaata attaaaggac tgaacatggc gaccacagta
     9121 atagctgcct ttaacgaatt tatgaaagat accgtgaatc tcaaaaaggc agataccgat
     9181 gacgcgcgtg caagtcgcga ctggcttatc ggtaagatga atgattttga gaaggacgat
     9241 aaatttccgg tgagttttcc agcgatccat attgcctttg gctcctttgc cagaagaacc
     9301 aaaatccgtc cgcttgatga tatcgatctg atgtttggct taaccgggca aggtgccacc
     9361 tataccatcc tgagtgaccg gataactgta acctcctccg gagaggggtc acgtctgcac
     9421 agttaccgcc attccggagc tgacaccgtc tgctccgtca gaattctgaa tgcctttaaa
     9481 aaccgcctgc aggacattgc ccagtacgcc caggctgata tcaggcgtaa ccaggaagct
     9541 gtcacgctga aactggtcag caaggactgg aattttgaca tcgtcccctg ctttattacc
     9601 agcgaagatg cctttggacg gacttactac ctgatccccg atggcaacgg ccactggaag
     9661 tttaccgacc cccgtaaaga cagggacaga gtcactacta tcaatgtgca gaataacggc
     9721 aatgtgctca atgtcatcag agcagtcaaa tactggcagc ggcgaccgac gatgccatcc
     9781 atgagttcct acctgctgga aacgctgatt ctggactatt acgctggcag gacatcctgc
     9841 tcatcgtttg ttgacatgga gctggaagcg cttttccgcc accttggtca atccgtgcgg
     9901 tactctgtca acgatcccaa aggcatacag ggtgatatca actcactttc agccgaggca
     9961 cgtaaggcga tatcggacag atgttatctc gatgcgcaaa aggtatcgga ggcgagatgg
    10021 tttgaaaata ataaagagta tgaaaaatcc atcaataaat ggcgcgatgt gttcgggcca
    10081 ttctttcctg tttacgggtg aatcatgaat gccatttaca caaaccagtc gaccagagaa
    10141 aacatggatt tactgtatgc gcaggcccgt gtctacgaca gggtaaaaaa ctggaacaga
    10201 ctgaattttc tcttcagcat catcgtaccg ctcctgcttt ctttcgttac cgtatacaac
    10261 cggagccggg aatttgtcga ttcagagttg ttgtcttcgc tgttggggtt atatggactc
    10321 ctggtgctta cctttaacat tgttatcagt ggacacatct cggctctgcg caggaaggca
    10381 gcctcaatcc aggaaatgta cgactgccgg gtacttggca tccggcgtaa tgaactgaag
    10441 gtggaggaga tcccacggga tgacatcatc cgggaagctg catattttag agacagcccg
    10501 gaaaaagcac gaaagcgatt tggcgaagag gggtggtacg tcagtaaggt gtatgacgcg
    10561 ccgcaggccg taatggcgct cctttgccac ggaaaaaatc tcggctggga caaatcgctc
    10621 agggaggttc tgcacgtatt ttatctttcc gcttttattg tctcgcctgt cgcgatgctt
    10681 gtttacggca tcgtcatgaa atccgggctg aatgagatcc tcttctatgt ggtgttcaca
    10741 ctgccggtca tccgctattt cttgcttcag tttctggata atcgcagcag tatgaaaaga
    10801 agcgagaagc tgaaaaagta cgttgagaag gaattatccg gcataagggt gtccggaagg
    10861 gcagaagaag agcaactggc ttatacgctc cggaatatcc aagatgagat gtttacctac
    10921 cgggcctcat gtccgcccgt acccaacggt attcagctga taatgaagcc caaaaatgaa
    10981 cgaatttatg ttgattactt tgaaacaaat ctgaaggagc tgcaccttca gaagtaatcc
    11041 ctccttagct ccggtgtagg atttacgtat gccggagcac atccgataaa caattcttgg
    11101 ttgcctgagt gtcagcgtaa acccatagtc accttcgcct cagacggtta gtaacacagg
    11161 taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg actttgtcat
    11221 gcagctccac cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc
    11281 tcagattcag gttatgccgc tcaattcgct gagtgtaacg cttgctgatt acgtgcagct
    11341 ttcc
//
LOCUS       DAEBXD010000114        11305 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEBXD010000114 DAEBXD010000000
VERSION     DAEBXD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.114"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KJL94_003354"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KJL94_003354"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBD4411584.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KJL94_003355"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KJL94_003355"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBD4411585.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KJL94_003356"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KJL94_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBD4411586.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="KJL94_003357"
     CDS             6038..6658
                     /locus_tag="KJL94_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBD4411587.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="KJL94_003358"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="KJL94_003358"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBD4411588.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KJL94_003359"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KJL94_003359"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD4411589.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KJL94_003360"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KJL94_003360"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBD4411590.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="KJL94_003361"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="KJL94_003361"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBD4411591.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KJL94_003362"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KJL94_003362"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2655 a         3251 c         2781 g         2618 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggca
//
LOCUS       DAEBXD010000115        11245 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEBXD010000115 DAEBXD010000000
VERSION     DAEBXD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.115"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            78..437
                     /locus_tag="KJL94_003363"
     CDS             78..437
                     /locus_tag="KJL94_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBD4411592.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(557..736)
                     /locus_tag="KJL94_003364"
     CDS             complement(557..736)
                     /locus_tag="KJL94_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBD4411593.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            810..2171
                     /gene="pabB"
                     /locus_tag="KJL94_003365"
     CDS             810..2171
                     /gene="pabB"
                     /locus_tag="KJL94_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBD4411594.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2175..2753
                     /locus_tag="KJL94_003366"
     CDS             2175..2753
                     /locus_tag="KJL94_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBD4411595.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2937..4301
                     /gene="sdaA"
                     /locus_tag="KJL94_003367"
     CDS             2937..4301
                     /gene="sdaA"
                     /locus_tag="KJL94_003367"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD4411596.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4432..6030
                     /locus_tag="KJL94_003368"
     CDS             4432..6030
                     /locus_tag="KJL94_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD4411597.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6034..7584)
                     /gene="yoaE"
                     /locus_tag="KJL94_003369"
     CDS             complement(6034..7584)
                     /gene="yoaE"
                     /locus_tag="KJL94_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBD4411598.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8047..9018
                     /gene="manX"
                     /locus_tag="KJL94_003370"
     CDS             8047..9018
                     /gene="manX"
                     /locus_tag="KJL94_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBD4411599.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9081..9881
                     /gene="manY"
                     /locus_tag="KJL94_003371"
     CDS             9081..9881
                     /gene="manY"
                     /locus_tag="KJL94_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBD4411600.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9894..10745
                     /gene="manZ"
                     /locus_tag="KJL94_003372"
     CDS             9894..10745
                     /gene="manZ"
                     /locus_tag="KJL94_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBD4411601.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10800..>11245
                     /locus_tag="KJL94_003373"
     CDS             10800..>11245
                     /locus_tag="KJL94_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBD4411602.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
BASE COUNT         2765 a         2839 c         2833 g         2808 t
ORIGIN      
        1 atgagctgct acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc atatcgttat
       61 aatccctttg atattagatg caaattaagg tcatatatag ccttatcgat aacatggtta
      121 attttaagga taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca
      181 tggatgtatc agcgccaaca ccaagttcga tgaatgaaag cacggcgaag ggaatcttta
      241 aatatttaaa agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc
      301 aggaaggctg gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa aaaatggaga
      361 caggtgaacg ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac
      421 taaaagcact ggtctgagtt aaatttatat cagcataaat gggtcaggac gcttttaatc
      481 acatataaaa agcgtccttt ttctcacaat caaatgatca tcggcttcta taacgaaatc
      541 gatacggatt agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt agctcgccgg
      601 agtggttggc acgcagctct tctgccacca gcgcgattgc ctggccgcta ctcatccctt
      661 gtgccatcag ttcctggatc cgctcgacag ctttttgctg ctgttcatgg atgagtgaag
      721 gtaaacctgc aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac
      781 ccggcacata gccagtagag tcaggactga tgaagacgtt atctcccact gtgattactt
      841 taccctggcg tcaggacgcc gctgaatttt atttctcccg cttaagccac ctgccgtggg
      901 cgatgctttt acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg
      961 ccgatccgat ttgcacttta accactttag gtaaagaaac cgttgttagt gaaagcgaaa
     1021 aacgcacaac gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag
     1081 acattcgccc aacgcataac gaagatttgc catttcaggg cggcgcactg gggttgtttg
     1141 gctacgatct gggccgccgt tttgagtcac tgccagaaat tgcggaacaa gatatcgttc
     1201 tgccggatat ggcagtgggt atctacgatt gggcgctcat tgtcgaccac cagcgtcata
     1261 cagtttcttt gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc
     1321 aattctcgcc gcaggaagat ttcacgctca cttccgactg gcaatccaat atgacccacg
     1381 agcagtacgg cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc
     1441 aggtgaatct cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc
     1501 ttcagcttaa tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg
     1561 caattttaag cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagaccc
     1621 gcccgattaa aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag
     1681 aaaaactggc gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc
     1741 gtaatgatat cggtcgtgtt gccgtagccg gttcggtaaa agtaccagag ctcttcgtgg
     1801 tggaaccctt ccctgccgtg catcatctgg tcagcactat aacggcgcga ctaccagaac
     1861 agttacacgc cagcgatctg ctgcgcgcag cttttcctgg tggctcaata accggggctc
     1921 cgaaagtacg ggctatggaa attatcgacg aactggaacc gcagcgacgt aatgcctggt
     1981 gcggcagcat tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca
     2041 cgctgactgc cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata
     2101 gccaggaaga agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac
     2161 tggagaagta agacgtggaa taccgtagcc tgacgcttga tgatttttta tctcgctttc
     2221 aacttttgcg cccgcaaatt aaccgggaac ccctaaatca tcgtcaggct gccgtgttaa
     2281 tccccatcgt ccgtcgaccg caaccggggt tgttgctgac tcagcgttca attcatctgc
     2341 gtaaacacgc tggacaagtg gcattccctg gaggtgcagt cgatgacacg gacgcatcag
     2401 ttatcgccgc cgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg
     2461 aagttatcgg cgtgctgccg cccgtcgata gcgtaactgg ctatcaagta accccagtgg
     2521 tcggcattat cccgcccgat ctgccgtatc gcgccagtga agatgaagtc tcggcggtgt
     2581 ttgaaatgcc gctcgcccag gcattacatc tgggtcgtta tcacccttta gatatctacc
     2641 gccgtggcga ttcacatcgg gtatggctgt cctggtacga acagtatttt gtatggggaa
     2701 tgaccgcagg cataattcgt gagctggcgc tgcaaattgg tgtgaacccc tgactatact
     2761 tatctttaca tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg
     2821 tttttgatta gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact
     2881 atgcgctgtt attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga
     2941 ttagtctatt cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc
     3001 ctatgaaggc gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg
     3061 ttactcgcgt tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca
     3121 ccgatatcgc cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca
     3181 gtattcccgg ttttattcgc gacgtaaaag agcgcgaacg tctgctgctg gcacagggac
     3241 ggcatgaagt ggatttcccg cgcgacaacg ggatgcgttt tcataacggc aacctgccgc
     3301 tgcatgaaaa cggtatgcaa atccacgcct ataacggcga tgaagtcgtc tacagcaaaa
     3361 cttattattc catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg
     3421 ccaacgaagt gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta
     3481 atgaaaccgg ctattcgctg tctggtctcg ctatgcagaa cgaactggcg ctgcacagca
     3541 agaaagagat cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc
     3601 gcgggatgaa caccgaaggt gtactgccag gcccgctgcg cgtgccacgt cgtgcgtctg
     3661 ccctgcgccg gatgttagtt tccagcgata aactgtctaa cgatccgatg aatgtcattg
     3721 actgggtaaa catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggtcgtgtgg
     3781 taactgcgcc aaccaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc
     3841 actttattga atcggtcagc ccagacatct atacccgtta ctttatggca gcgggcgcga
     3901 ttggtgcatt gtataaaatg aacgcctcta tttccggtgc ggaagttggt tgccagggcg
     3961 aagtgggtgt tgcctgttca atggctgctg cgggtcttgc agaactgctg ggcggtagcc
     4021 cggaacaggt ttgcgtggcg gcggaaattg gcatggaaca caaccttggt ttaacctgcg
     4081 acccggttgc agggcaggtt caggtgccgt gcattgagcg taatgccatt gcctctgtga
     4141 aggcgattaa cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc gtctcgctgg
     4201 ataaggtcat cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa taccgcgaaa
     4261 cctcacgcgg tggtctggca atcaaagtcc agtgtgacta atacttctta ctcgcccatc
     4321 tgcaacggat gggcgaattt atacccgctt tctcgtctgc tgtaatattc cccactacac
     4381 ttccactgtt gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa
     4441 gcacaacgga tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca
     4501 ctcgttacgc tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa
     4561 caacgggtag tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc
     4621 ctacaggcag gtagcaaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat
     4681 ttgccattac gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa
     4741 gacggcacac tttattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt
     4801 ctggctgaac ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg tgccctgatt
     4861 aaaggcagtc cggttttgat tcaatggacg cctgcagcgg gcagtagcaa tgctggggtc
     4921 atagagatga ttaacatcga cttactgacg gcaatgctcc ttgagccaca actgccgcaa
     4981 atcagtagcg ccagcctgac ggtggacaaa cggcatttgc tctatggtaa tgggctggta
     5041 gattcccttc cgcaacctga agacaatgaa aactatcagg tttcttcgca aagctttcct
     5101 tttaccatta acgtaaatgg tccgggggct acggcgctgg catggcacta tcttccaaca
     5161 caattaccgc tggcggtgct gctaagttta ctggtgggct atatcgcctg gctggcgacc
     5221 gcttaccgga tgagcttttc ccgcgaaatt aatctgggcc tggcgcaaca tgagttcgaa
     5281 ttgttctgtc agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg
     5341 ctgcgctgga ataatccgcg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg
     5401 gaagaacatc atttaattgt gccactgacc cactatgtga tggcagaaac cattcgtcag
     5461 caccatgttt tcccgatgag tagtcagttt catgttggca ttaacgtcgc acccagccat
     5521 tttcgccgtg gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt
     5581 cagcaactga tcctcgaaat caccgaacgc gatgccttac tggatgttga ttatcggatt
     5641 gcccgcgagc tgcatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac
     5701 agctcgtttt cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt
     5761 accgcagcta taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcgctg
     5821 gggcaaagac tgaatattga actggtggcg gagggcgtgg aaacgcaaga acaggcgaag
     5881 tatttgcgcc gtcatggcgt gcatattttg caagggtatt tgtacgcaca gccgatgccg
     5941 ctacgtgatt ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga
     6001 catatcacgc ccattatgtc gttacgttaa cgcttactca tcttcatcgt gcgccggttg
     6061 ctctttaaca atgcgaacca gatcaacacg ataatcattg gcttcaagga tggtgatatg
     6121 cagtggccct acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac
     6181 gaggcccgcg accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg
     6241 ctgcaaggca tgcaaatctg taccgccttt taccagccag ccgttaccat cggtaatgat
     6301 ttccggcgtt tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg
     6361 cgtgaccaag ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc
     6421 gcgacgcagc acgcccagca ggttgatcgg atcgagggtt tccgggacga taatcgccgg
     6481 agacgccgaa gcaatcgccg ccacatcaac gccctcttcc agcgccacca gcagttcttt
     6541 agcacgtacg ataccgatga tttcatccag ttcaccgcga cataccggga acagactgtg
     6601 cggtgaagag agcagttgct cgcggatttc atcgaccccg agattagcgt caacccagct
     6661 tatttcaccg cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tcagtacgcc
     6721 gttaatcatg taccgttctt cttcggcaaa tgcaccttcc gggatcggca tcgggttatc
     6781 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc
     6841 agtacgcgct cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg
     6901 gttaaacact tcaataatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
     6961 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
     7021 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggtttcg atgccagcaa
     7081 cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc
     7141 tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac
     7201 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
     7261 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat
     7321 cagatcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac
     7381 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag
     7441 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac
     7501 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
     7561 tgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
     7621 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa
     7681 aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
     7741 actgccctat actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt
     7801 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
     7861 ttctgataat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
     7921 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
     7981 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
     8041 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
     8101 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
     8161 ggtgaaaatg ccgaaacgct gattgaaaaa tacaacgctc agttggcaaa actcgacacc
     8221 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc
     8281 cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc
     8341 gtggaaacgt taatggcccg tgatgatgac ccgagctttg atgaactggt tgcgctggca
     8401 gtagaaacag gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg
     8461 ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatggtgcca
     8521 aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc
     8581 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
     8641 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
     8701 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
     8761 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
     8821 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
     8881 aacgcggttt cggttgatga aaaagatatc gaggcgttca aaaaactgaa tgcacgcggt
     8941 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
     9001 agcaaaatcg ataagtaacg tattgtgttg attatcattc agttttcaca cttaagtctt
     9061 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
     9121 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
     9181 tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg
     9241 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg
     9301 ccgctctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg
     9361 caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc
     9421 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
     9481 cagcgatttc ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc
     9541 cggccgtcat cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
     9601 ttccggaagt ggtgaccaat ggtctgaata tcgccggggg catgatcgtg gtggttggtt
     9661 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
     9721 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
     9781 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
     9841 aggcagctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
     9901 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtattcc
     9961 tgcgttctaa cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    10021 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    10081 aagctattcg ccgtcacctg gagttcttta acacccagcc gttcgtggct gcaccgattc
    10141 tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg
    10201 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    10261 tctggggaac cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca
    10321 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    10381 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    10441 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    10501 tggttaacaa gtggacacat gtcaacatcc cgctggtggt ttcgaccatc accgggcagg
    10561 atggacagac ccgtgtcacc accgtgcaaa ctattcttga ccagttaatg ccaggccttg
    10621 taccactgct gctgacattt gcttgtatgt ggctgctgcg caaaaaagtt aacccgctgt
    10681 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    10741 tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    10801 tgacaatcac ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct
    10861 acgatcagtt catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    10921 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    10981 acgtcacgaa tcatggcgcg ctaataacaa catggttatt aagtgcactg gctctgatgg
    11041 gtttttatat attctggatc cgcattccga agatcatctt taaacaaaaa ggttttttct
    11101 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    11161 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc
    11221 tggaaaaaat ttataagctt ctcgt
//
LOCUS       DAEBXD010000116        11084 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEBXD010000116 DAEBXD010000000
VERSION     DAEBXD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.116"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            404..562
                     /locus_tag="KJL94_003374"
     CDS             404..562
                     /locus_tag="KJL94_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBD4411603.1"
                     /translation="METLSTNLQLARLVGVQGTPATIIGDEMIPGAVSWETLEAVVKE
                     KLAVAHAQ"
     gene            552..1058
                     /locus_tag="KJL94_003375"
     CDS             552..1058
                     /locus_tag="KJL94_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HBD4411604.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            1241..2056
                     /locus_tag="KJL94_003376"
     CDS             1241..2056
                     /locus_tag="KJL94_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HBD4411605.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2403..4289
                     /locus_tag="KJL94_003377"
     CDS             2403..4289
                     /locus_tag="KJL94_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HBD4411606.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4330..4857
                     /locus_tag="KJL94_003378"
     CDS             4330..4857
                     /locus_tag="KJL94_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HBD4411607.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4961..6340
                     /locus_tag="KJL94_003379"
     CDS             4961..6340
                     /locus_tag="KJL94_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HBD4411608.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            6343..7626
                     /locus_tag="KJL94_003380"
     CDS             6343..7626
                     /locus_tag="KJL94_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4411609.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            7616..8746
                     /locus_tag="KJL94_003381"
     CDS             7616..8746
                     /locus_tag="KJL94_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4411610.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALSAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKMVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            8751..9446
                     /locus_tag="KJL94_003382"
     CDS             8751..9446
                     /locus_tag="KJL94_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4411611.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            9433..9918
                     /locus_tag="KJL94_003383"
     CDS             9433..9918
                     /locus_tag="KJL94_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBD4411612.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAVFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPPELEATFARLAA
                     R"
     gene            9943..10428
                     /locus_tag="KJL94_003384"
     CDS             9943..10428
                     /locus_tag="KJL94_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411613.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            10550..>11084
                     /locus_tag="KJL94_003385"
     CDS             10550..>11084
                     /locus_tag="KJL94_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HBD4411614.1"
                     /translation="MKKLILAAAVAGAVLLSSAAQAQTTPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNQWSEWDVPGGQLTTKGGVLEIYMGHYMREWLAELGMVTSGECP
                     TPDTVYTYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SQKAVQAMEKERSQMQLD"
BASE COUNT         2455 a         2738 c         3335 g         2556 t
ORIGIN      
        1 tcggggcaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat cttgttgtct
       61 gattattgat ttttcgcgaa accatttgat catatgacaa gatgtgtatc caccttaact
      121 taatgatttt taccaaaatc attaggggat tcatcagggt gatgcgtgca ctacgcaaag
      181 gccaggcctc agcattttat tatggtgatc ccctgggcga aatgcgcctg gtaagcagag
      241 tttttgaaat gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta actttgcaac
      301 agtgccgtgc agacagaaac cgtaaacacc ggcattgaca tgccggggaa gcccttatag
      361 tacaataatt ctctatatcc aaactgaccc cgatgagaaa agcatggaga cgttgagcac
      421 caatctgcaa ctcgcccggc tggttggcgt tcaggggacg ccggcaacca tcattggcga
      481 cgagatgatc cccggcgcgg tatcgtggga gacccttgag gcggtggtga aagaaaaact
      541 ggcggtggcc catgctcagt aaactgcgcc gctggctgcg tgaaggcgcg atcttactgg
      601 ttgtgctggc tggcgtgata atcctgctgg atgtctggcg ttctccgcag atgccggcca
      661 tgtttgacag cacgccgctg catacgctgg acggggaaac cgtaacgctg gcgtcgatca
      721 gtgaagaacg cccggtgctg ctctacttct gggcgagctg gtgcggtatc tgccgtttca
      781 ccacgccgga tgtcgcccgt ttgcagtcgg aaggggagag cgtcatgacc atcgcgctgc
      841 gttccggtaa tgacggggag gtttcccgct ggttatcgcg taaaagggtg acgttccccg
      901 tcgtgaatga tagcggcggt gagatatcac gaaactggga aatcagcgtc acgccaacgc
      961 tggtggtggt gtcgaagggg caggtggtaa ctacgaccag cggctggacc agctactggg
     1021 gcatgaagtt acgtctgtgg cgggcagcca tgttttgata gcgtgccggg gaaacccggc
     1081 attttttaac ccattgtaag gatgtgtaaa acgagcggag agcgtgtttt ctgttatttt
     1141 tgctgctttt ttaccgacgc cagttattat tttttaccgc tgcctgacgt tgcaaacctg
     1201 aattgataat ggcatatctg ttttaattga atgaaagtct gtgatacgtt caggcgattt
     1261 tttcgatttc cattttgaca gggataacaa ttcaatgcgc aagggtaaaa ttattgttgc
     1321 atcaatgatg gtgctttgtg tccttatttt tgttgcggcg gcggcatggt ttatgctgtt
     1381 ccagggagaa atgacggagc cgacagagga ggaaaaacag gcggcaattt tacacgctgc
     1441 gggcctgatg aaatcagaga cccaggataa aaaatcagtc gaaacgctct atcatcgtta
     1501 tattattcaa cgccatgtta atttagattc cggcgaactg gtggctggca gcagcgcgga
     1561 caccgcgcgg cagaagtgtg aaaaacttgc ccctgaacgc gacccagcgc aggttcgtca
     1621 gcgctgtacg gtggcggatg tgttctttgt gaaagataaa aataatgaaa ttcagcaggt
     1681 tatcattcca gttaccggta agggtgcgaa atccatgatg catgcgtttc ttgctctggg
     1741 gctggacggt cgtacggtga gaaatctgta ttattatcag caacgggaaa cgccgtttct
     1801 gggggctagg gtggaagacg ccaactggcg caaacagtgg ccagggaaac gattattaga
     1861 taacagcgga catcctgcat tgaaaatagt acaggataaa cctgaacatg ccgatgaata
     1921 taccgttgac ggtatttccg gtgcgacgtt gacatcaacc ggcgttgaga aaagtattaa
     1981 ctactggatg gggccgcagg gatatggtca attcttacag cgtcttgcca gcgatcgaaa
     2041 taaccttaat ctttgaatat tgctgtacgc aataacgcgt tttatttctg cctgccgata
     2101 atgaataata tcactgtcgg cagggggatt tattcctctg ttaccgcgcc tgccttgccg
     2161 ttaattccat gagaaaaaca gggagataac gtccctttta tcaacgcata atgtgtgtgt
     2221 tcatcacgat ttgatgaaat aagtttacat aacttgtcgt ctgctgacat ttttatgatc
     2281 caggtcatgt ataacgcgac aaaaagatat gataataatt atcatttgta ttctttggcg
     2341 gtgtgcacgt gcgtaacttc cgtaactctt tgttggttac tctctttggc ttgtttataa
     2401 gcatgagtgt ctgggcgtcg acgaattatg cgcctttgat tgaagatatc gagcaacgtc
     2461 ttgataaaac agcggaactt tatcagcaac agcatgccga cgaggcgcgc cgcacggtac
     2521 aaatggccta ttttgaggtg ttcgagaacc tggaagggcc gatccgcatc aacatttccg
     2581 cgcgcaaaag ttacgagatg gagagcgcat tcggcgagat ccgtcgaatg attggcgaga
     2641 aaaaaccgct cgctgacgtc caggcgcgca ttgactggct gaaagccgca ctgcgcgaag
     2701 tggaaccggt gctggatggc ggacatcgtc tggtggccga agagcagcac aacgcgctgt
     2761 ccagggacga cattgccgtt cactggcagg agagtttcag gacgatcgac gatctgctgg
     2821 cgcaggcggt cactgaatat caggccggga attacagcgt tgccagtcag cacgttcaac
     2881 aggcgcacta tcagggcttt aaaaactctg agatggagat gtccctcaga caaaaccgtt
     2941 ctgcaaaaga tgccgcctcc attaaccaac aattctcatc cctgattgcg ctgaccgcgc
     3001 aacctgacca tctcaacgat gtctcctatc aggtcactac gctgctacag gatatcgagg
     3061 atatcttacc cggcttgcca accacccgtg acgatcagca ggttgccgca gcgcaaagcg
     3121 cagacaacat tccggcggcg gacgagggaa ataaccgcac cgactgggct gaggtgacga
     3181 cgggaattaa ccagagcatt caggacgcga tagcgcgcta tcagggcggc gatgcgaaga
     3241 atgcgattct ggatatccag gatacctact tcgaccgctt cgaagccagc ggcatggaaa
     3301 acaaaatcgg ctctcgcgat tcggcattta aaaccacgct ggaggcgtat tttacccgcc
     3361 tggtcagtct gatgaaggcc ggtcagccgg ttgagcgcct gaatgcagag gccagcgcgc
     3421 tgacgcagga tctgcaaaaa gccgtgacga tgctgggcga aggcgaagaa acccagtgga
     3481 gtctgctgct ctatagtctg atgatcatcg tgcgcgaggg gctggaagcg ctgctgattg
     3541 ttgcggcaat cgtggcctac atggtgaaaa acaaccatca ggataagctg ccgcttattc
     3601 gtcagtcggt aattgtggcg ctgatcgcca gcgtcatcac cgccgctatc ttccagatgc
     3661 tgtttactaa ctccggcgcc agccgtgaac tgctggaagg cataacgatg ctgattgccg
     3721 tcgtgatgct gttctttatg agttactggc tgctgtccaa agtggaagcg cgccactgga
     3781 aagcctggct ggaaggcaaa ctgtctcatt ccctgtcgcg aggctccctg gtcggactat
     3841 ggctgaccag cttcctggcg gtgtaccgcg aaggggcgga aacggtgctg ttctattacg
     3901 ctctgattgg cgatgcaaac acggtatccg gccatatggc gattggcgcc ggttttgtga
     3961 ttggctgcgt ggtgctgctg ctcgcctggc tggtgatgcg ttactcagtg gtgcgcctgc
     4021 cgctgaaacc cttctttatg tttaccggca gtttcatgta tctgatggcc ttcgtctttg
     4081 cgggcaaagg cgttctggaa ctggtggaag gcaaactgtt ccagcctacg ttgattaacg
     4141 gcttccctga aatcagctgg ctggggattt atccctacgt ggaaacgttg ttaccgcagg
     4201 tcgtattgct gctcgcggcg ctggtggcgc tgtgggtgat gcggcgcaaa agcgcggttc
     4261 ccggggagac gataaaaaat aacccataat cgttgtatag ccgttgtttt agacgagagg
     4321 atacgtttga tgaccatgaa gaaaaccctg attgccagcg cagtgatggc cagcattttc
     4381 attgcgccag ccgcgtttgc ttttaaagag tacccggcag gcgagcctgt caccatgaat
     4441 gaaatggagc tggcggccgt ttacctgcaa ccgattgata tggaaccgcg cggcatgggc
     4501 ttaccggcag caaaagccga tgttcacctt gaagcggata tccacgctgt agaaggcaat
     4561 aaaaacggct ttggcgcagg ggaatggatc ccgtacctga ccatcagcta caccctggtc
     4621 aacaacgaca cgggcgaaaa acaggaaggc accttcatgc cgatggttgc cagcgatggc
     4681 ccgcactatg gcgcgaacat caaaatgatg ggcgtgggca actataaagt gacctaccac
     4741 attgaaccgc cgtcaaaagc cgggatgcat cgccatactg acagcgaaac aggcgtaggc
     4801 cgctggtgga aaccgtttga tgtgagctac gagtttaaat atgtcggtct gaactaaaac
     4861 cgactaacca acgcgccagg aggattcccc cttctggcgc gttggtcaga ccgatcatat
     4921 gcccgtcgct taattcaacc cgccgtacag agtcgaagtt atgagttact ttttcgtcac
     4981 gacgctacag gtctttttct gcattgcgct tctttccggt gttctctgga gtcgaaacga
     5041 tcctccctct ttacgtccgc tgacctggac gctgctgacc gggctgattg ccggcgtgct
     5101 ggcgggactg tttattcacg gcagccaacc ggtacaactg ctgctggttg gggcggaggt
     5161 catggtatcg ctgctgtttg tcctcagttt ctggtgggct tcaacgcgta tccgctatct
     5221 gtggcagggg attctgattt tcggcgctgc ccgccattgg gcgctggacc ctaatctggg
     5281 cgggctgacc agtacgcatg tgctgaatac tgacctgctg ttaaacctga cggccgtcgt
     5341 tctcgctttt gcgatcctct gtctggcggg cgtactctgc gcgatgctgc tgcggcgtat
     5401 tcgcggcctg tactggccgt taacgctgat tctgctggtg atgatctggc tgccgctgag
     5461 cggcaacctg ctgttgttgc tgatgaaatt acaggttgtt ccgctgggaa aatcactgct
     5521 gagcttcgtg gcgaaagtca caaataacac ggcgctgtat aactgggcgg gagccgcgct
     5581 gctgctggcc ctggcgctgt gctggcttcc ggcgctgttg cgggcttttc gacagacgcg
     5641 ggaaaccgaa gaaccgattg ctcaccgtct ggcgctggcg cagcgtcgca acgctttgcg
     5701 gctgtggttg gtcacaatcg gctgcgcggt ggtcgttatc gcaggccaac tgtggtggga
     5761 taaagtggcc tcgcagccgc cgcaactctc tgaagccgtg cccgtaacgc tggggagcga
     5821 tggcatggtg cgcctgccgg ttgaacagct tcgtgacggt aaactgcatc gtttcgtctg
     5881 ggtggcggac gatggcaaag cggtgcgctt cttcgtgatt aaccgctatc ccgacaagct
     5941 gcgttttggc gtggtgttcg acgcctgcct gctgtgcggc gatcagggtt atgtcatgga
     6001 gggcaatcag gttatctgcg tcgcctgcgg agtgcatatt tttattccgt ctatcggcaa
     6061 ggccggtggc tgtaacccgg tgccgattga aaactggcac aatgatgaaa aagagctggt
     6121 gatccccggt aaagaactgg cgacaggggt gaactacttc tcgacggtca tgactattaa
     6181 agtgaccgat ccggtcgatg gttcaacgtt gaccaatacc tcagccgatt acaaatacag
     6241 ctatggcggc aagacctggt tcttctcgtc agaggctaac tatgaacgct tccgtgaaac
     6301 gccggagcag tttgttcctg ccgacatgag ggaggaataa tcatgctgtg gcggatgtta
     6361 cgacaatcct gggggcgcaa tttacgccgc aaagtgctgg cgataatcac cgtttttctg
     6421 gcctccagcc tgatctcggc gctgctggcg gtgtccattg atatcggcga caaaatgtcc
     6481 agagagctga agtcttatgg tgcgaacatt ttgattgaac ccgccggaca ggctgcgctg
     6541 cccgcgctgt ttagtgagag cagtaatccg ctttccggtc aggattttct tgatgaagcc
     6601 gagctgccga acatcaaaga tatcttctgg cgaaacaaca ttgtcgggtt tgcgccgatg
     6661 ctcggcggcg aagccagcgt ggagggagaa ccagtgcgga ttctgggcac tttcttcagt
     6721 cagccagtgg atattcctga tgaagaggga tatgagacgg ggcagaaaac ggtcagccca
     6781 tactggcagg tgaccggcga ctggccgcag gaacccgcag gcgcagagcc gcagacctta
     6841 gtcgggcacg cgctggcgcg gcaaatgggc tggaaacccg gtgataagct gacattacgt
     6901 accgaaggcg aagcagtgca ggtcaccgtc agcggtatcc tctccagcgg cggcgatgaa
     6961 gataaccaac tggtgatgcc gctaagcacg gtacagcacc tgctggggct ccccggaaaa
     7021 gtgcaggcga ttcgcgtctc ggcgctgacg gtgccggaaa acgaactgtc ccgccgcgcg
     7081 cgggaaaacc tggacgcgct caatgccgaa gagtacgacc tctggtattg cacggcctat
     7141 gtgtcgtcga tcgcgcatca gctggaagag gcgatttccg gtgcggaagt gcgcccggta
     7201 tggcaggtcg cggcgtccga aggggtggtg atcgataaaa ttcagttgct gatggcggtc
     7261 gttaccgtcg cggcgctggt ggcttccgca atggggatcg cctcgctgat gaccagcacc
     7321 attatggaac gcgccaaaga gatcggtttg atgaaagcgc tgggtgcgcg acagtggcaa
     7381 attatgctgc tgttctacct ggaagcggcg tccagcgggc tggctggcgg tgcgctggga
     7441 tgtattgccg ggtgggggct ggcgaaagcg attggcgtca tgctcttcga cgcgccgctt
     7501 aattttgcct ggattgtggt gccgtgcgta ctggtgattg cggtactgat tgcgttgatc
     7561 ggcacctggt tcccggcccg gcggattgcc cggctgtatc ctgtggaggt gctgtatggc
     7621 cgctaaacgt accatgctct ggctgctggt gtggcgggcg ctgcgtctgc gttttcagcg
     7681 ggtcagcgtg gtgtttgccg cgctgatggt gggggcgacc atcgtgacgg cgctttctgc
     7741 cgtctggttc gacattaaca ccaaaatgag cgaagaactg cgcaccttcg gcgcgaattt
     7801 ttacatcggg ccggggcacg gttccagtat gccgcagcag gagttgcaga gcattctcga
     7861 tcaggcgccg caggggctgg tgcatggcgc cagtccgtgg ttgtacggca tggcgcgtac
     7921 cgagctggaa aaagtggtga tggttggcgt gtggttcgag tcgctgcaaa aaatggtgcc
     7981 gtactggcag gtcaccggta gctggattgg cgtcagtttt gacgaccgta atgcgatgat
     8041 cggcgtcaaa ctggcggaac gcctcaacgt gcagccgggc gacagcatca cgctggtgga
     8101 tcacaatcag cgtaagaatt tgcagattaa aggcattgtg gagtctggcg atgcgacgga
     8161 caacatgctg atcgtcagcc tcgacgtggc gcaggcctgg ctgcatcagc cgggcaaaat
     8221 cagccacggc ttgctgagcg tcagtaacga tgtcggccag gtggagaact atgccagccg
     8281 gttgcaggcg cagtatcctg acctggagat tcgtccggtg cgtaaggtct ctgcttccga
     8341 agggcaggtg ctggataaaa ttaaaggtct gatgggactg gtctcgatcg tgattctggc
     8401 gctctcttcg ctgtgcgtga ataccacgct gatggcgatt gtcggcgagc gggcgcgcga
     8461 gtttgcgctg caaaaagcgc ttggttccag caacggcgat atcgtgcggc aaattctgct
     8521 ggaaaccagc atcatcgcgc tggcggcggt ggcgtgcggc tgggtgctgg gatatttgct
     8581 ggcgcaactg ttgggattaa cggtgtttaa tgccgccata tcactgcgtc tgccggtgtt
     8641 gcccattact ctggtgttgt ctttactggt cgccattctg gcggcgattg ttccggtgcg
     8701 acgcgcggta agcgtcgagc ctgctaaagt gctgaaagga gagtagcgcc atgtccgtta
     8761 cgcatatccc tcaggtggcg atcgaaaccc gccacttata caaacggttc ggtgacgtta
     8821 ccgccctgga tgacattaat ctgcgcattg cgcagggtga atttgtcgcc attatgggcg
     8881 cttctggttc tggtaaaacc acgctgatga atattctgac gtgccttgat acggcgacgg
     8941 aagggcaggt gtttcttgat ggcaccgatg ccgccgcgct ggatgaagag gggcgccgtc
     9001 gctttcgcgc cgagaaaatc ggcctggtat ttcagcagtt ccatttgatc ccgttcttaa
     9061 ctgcgctgga aaacatcatg ctcgcccagc attaccacag cgtggtggat gaagcggcgg
     9121 cgaggaaggt gctggaacag gtagggcttg ggcatcgtgt gacgcacctg ccgagccagc
     9181 tttccggtgg tgagcagcag cgtgtgtgta tcgcccgggc gctggtcaac gaaccgccgg
     9241 ttattttcgc ggatgaaccg acgggcaacc tggatgaaga gaatgagcag cgggtgctgg
     9301 atctgctcac cgatctgcat cgtcaggggc gcacgattgt gatggtgacg cacaaccctg
     9361 cgcttgggca gtttgccgac cgtattctgc ggctgcaaca cggtaaatat ctgggtgagg
     9421 aggccaatca acatgcgctg gcataatgtg ctgttactcg cggcggcgat cctgataagc
     9481 gggtgtaaag aagaaaagct ggcggtcggg gcaccggccc cggcgctggc ggtgtttgat
     9541 ctgcaaggcc aggagtcagg ccttgaacgc tggcaaggaa aggcggtgta cctgaatttt
     9601 tggtctgcgg gatgcggggg gtgcctggcg gaaatggaca cgctggaagc gctgagcaaa
     9661 aaatggggcg ataaagtggt ggttgtggcg gtcaataccg accctgaaac ggtcaggatc
     9721 aacgatctgc tggcaaaaca tcagataacc tatccggtgg tgcgcgatca gctcaacatt
     9781 acccaggaac gttatcaggt gatcggcacg ccgacctccg tactgattga ctccgaagga
     9841 cgcgttctgg agctacacca gggtatgcgt aagccgcctg agttagaggc cacgtttgcg
     9901 cgcctggcgg cgcgatgaac gacgcgtacg gagagatacc caatggcatt tatctggaac
     9961 gacgaaagcc ttgcgattct gcgggaaaac gcgggcatcc tcacgacaga gcagatagcg
    10021 caactgcttc acactaatat taccgccgtg cggaatatgg cctatcggct gaaactgagc
    10081 cttcgggtga cggcgtataa tcaccgacgc atagcgcagg ttcaggcgct ttatgcctct
    10141 gagacgttga gtctcaaaga gatcgcggcg aaaacggggc tgacggccag taccgtgcaa
    10201 tatatcgtgt acgtgaaatc aaaaaataag ccctacgcca ccacggaata tgtctctttt
    10261 gaaacggaga acgccgttca ttaccgcgtg caaaaagaat ttgtcgatac ggaacgttcc
    10321 ctgctggaca acatctctga taacacgcga tttcgtgaac tgtatctgac ggacggcact
    10381 ttttattgcg cccgcaatat taagtatgag gtttttatct cggagtaacc ggaacacgct
    10441 gacctgccgg ttatcttctg gaatctcccg ttttgcgaaa ccgttcccgg aaaaatgtca
    10501 tatttctgtc acactctatg aggattgata acaacaagag gttgtaggga tgaaaaaatt
    10561 gatactcgcc gccgctgtgg caggggctgt tttattgtcg tccgccgcgc aagcgcagac
    10621 aacgccggaa ggttatcaac tgcaacaagt gttgatgatg agccgacaca acttgcgcgc
    10681 gccgttggcc aataatggca gcgtgctgga gcagtccacg ccgaatcagt ggtcggagtg
    10741 ggatgtgccc ggcgggcagc tcaccacgaa aggcggtgtg ctggaaattt atatgggcca
    10801 ctacatgcga gaatggctgg cggagctggg gatggtcacg tcaggcgaat gcccgacgcc
    10861 ggacacggta tatacctatg ccaacagtct gcaacgcacc gttgcgacgg cacaattctt
    10921 tatcaccggc gctttccctg ggtgcgacat tccggtacat catcaggaaa aaatgggcac
    10981 gatggaccca acgtttaatc cggtgatcac cgatgactct gcggcgttta gccagaaagc
    11041 ggtgcaggcg atggaaaaag agcgcagcca gatgcagctt gacg
//
LOCUS       DAEBXD010000117        11069 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEBXD010000117 DAEBXD010000000
VERSION     DAEBXD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.117"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            88..849
                     /gene="adiY"
                     /locus_tag="KJL94_003386"
     CDS             88..849
                     /gene="adiY"
                     /locus_tag="KJL94_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBD4411615.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            986..2323
                     /gene="adiC"
                     /locus_tag="KJL94_003387"
     CDS             986..2323
                     /gene="adiC"
                     /locus_tag="KJL94_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBD4411616.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2427..4070
                     /gene="eptA"
                     /locus_tag="KJL94_003388"
     CDS             2427..4070
                     /gene="eptA"
                     /locus_tag="KJL94_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBD4411617.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4067..4735
                     /gene="basR"
                     /locus_tag="KJL94_003389"
     CDS             4067..4735
                     /gene="basR"
                     /locus_tag="KJL94_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBD4411618.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4736..5836
                     /gene="pmrB"
                     /locus_tag="KJL94_003390"
     CDS             4736..5836
                     /gene="pmrB"
                     /locus_tag="KJL94_003390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBD4411619.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="KJL94_003391"
     CDS             complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="KJL94_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBD4411620.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6013..7515)
                     /gene="proP"
                     /locus_tag="KJL94_003392"
     CDS             complement(6013..7515)
                     /gene="proP"
                     /locus_tag="KJL94_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBD4411621.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7779..8657)
                     /locus_tag="KJL94_003393"
     CDS             complement(7779..8657)
                     /locus_tag="KJL94_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBD4411622.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8654..10882)
                     /gene="crfC"
                     /locus_tag="KJL94_003394"
     CDS             complement(8654..10882)
                     /gene="crfC"
                     /locus_tag="KJL94_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBD4411623.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2780 a         2878 c         2705 g         2706 t
ORIGIN      
        1 tattcatcat gcaattatat aaccatttcc cggtatcgct ttgctttagc gagaactggt
       61 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt
      121 ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa
      181 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaac
      241 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      301 aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact caccctgcca
      361 tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc cgcagaaact
      421 gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg ttttctcgac
      481 agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      541 tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt agccagttgt
      601 ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac cagttatagc
      661 caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat ggacggtaaa
      721 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      781 tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag agaacgcact
      841 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      901 gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt attgtcacga
      961 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
     1021 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1081 tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga cgattatcgg
     1141 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg
     1201 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1261 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1321 aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct gcgtcgtggt
     1381 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gcgtgcaggc
     1441 cgttgccact gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1501 gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc
     1561 gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1621 tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca ttggtggggt
     1681 attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga tgatccctaa
     1741 tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1801 caccgctggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1861 ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc
     1921 gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta tcgtcggtat
     1981 tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag agttcggtct
     2041 ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2101 actgctgctc ggccacggtc actttggtaa agcacgcccg gcatatctgg ctgttactac
     2161 cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag aggttatgtg
     2221 gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca accggctaca
     2281 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatccagca
     2341 aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2401 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga
     2461 atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg aatattgcct
     2521 tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta ctggttttct
     2581 tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta agctctttct
     2641 tatggcttaa tcgaccactg gcctgcctgt ttattctgat tggcgcggct gcacaatatt
     2701 tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt attgatacca
     2761 ctccagcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg ggattcagcg
     2821 gcgtgcttgc tgcgctgatt gcctgctggc taaaaatcaa acctgccacc tcgcgtctgc
     2881 gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt ttgctggtcg
     2941 ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag ctggtgaaat
     3001 ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat cagcgactgg
     3061 caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccgtta atgcagaacg
     3121 aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg gagaacttct
     3181 ccctcaacgg ctacccgcgt gcaactaacc cgcggctggc gaaagataac gtggtctatt
     3241 tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc atgttctcgg
     3301 atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3361 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggtg
     3421 cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctgat cagtgcatca
     3481 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3541 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3601 gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag atccagacct
     3661 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3721 ttgataaagc gattaatctg cttaaagaac atcaggataa atttaccacc agcctggttt
     3781 atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt
     3841 atgccatcgc cccggatagc caaaaacagg tgccaatgct gctgtggctg tcggaggatt
     3901 atcaaaagcg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact
     3961 attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     4021 accaggccgc ggatgatatt ctgcaaactt gcaggagagt gagtaaatga aaattctgat
     4081 tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa ccgaaggcta
     4141 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg acggtcatta
     4201 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4261 tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg atacgctgac
     4321 cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc cttttgcgct
     4381 ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4441 tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat ggatgggcgg
     4501 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4561 aggcagtccg gtacatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4621 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4681 tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa actaattgaa
     4741 tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgaccatcgg
     4801 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4861 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4921 gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggtcagcct
     4981 gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg cggagctgca
     5041 aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc attgccattc acagcgccac
     5101 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5161 gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5221 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5281 agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac tggcgcgtgc
     5341 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5401 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc tgctattgcc
     5461 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5521 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5581 gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta ttgatgagag
     5641 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5701 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5761 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5821 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5881 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5941 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc
     6001 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc
     6061 ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat tatcgtaatg
     6121 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa
     6181 cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca ccgccactac
     6241 catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6301 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6361 acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgccca taaagcagtt
     6421 aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat
     6481 cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg
     6541 acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6601 cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa
     6661 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6721 aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg agactttcgg
     6781 gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct gctggaacgc
     6841 cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat
     6901 aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat
     6961 taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     7021 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt
     7081 atattcacca ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7141 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7201 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7261 gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7321 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7381 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7441 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg gttttacttt
     7501 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7561 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7621 tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7681 attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc aacttcacac
     7741 atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt tacagagaag
     7801 atttcctcga ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg catccccagc
     7861 gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggtc
     7921 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac ggtcgaagag
     7981 gcaaaccact gatggtaata gtgacgcgta gtcggtgccg cattgatact ctctgccaga
     8041 tgttgtgtcg ctttctcgcg caaatcaccc agcaacaaca ccaccgcttg cgggcttccc
     8101 agacggcagg catcgccaaa tgcaccccag cgcaactcct ccagaacgat aaaacagcgc
     8161 ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat ttgctcgctc
     8221 tgatcacctg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8281 cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg gaaagcagag
     8341 tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc
     8401 agatgaccgg gatccagcat cgacgccagt ttttccacca ttgtcagtcg caggctaagc
     8461 ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacacgcga aagaagttcc
     8521 tgcattagtc gttcacgaaa ctgctgctga tgggcttgag gatggcgggc atcatcgccc
     8581 tgcgccagat ccactaaaaa gcgcggatga accgactcca gcacgcgacc ggggccgtcc
     8641 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8701 gctgagcgtc ggtgtaaatc cagctgtgag tcatcacagt ctgctgcaat tgctgctgca
     8761 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8821 gtaattctgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8881 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaatagaga tcgacttctg
     8941 cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat ttctcatttc
     9001 cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac
     9061 gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac agctggcgag
     9121 tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg
     9181 actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg gttaacaccc
     9241 ggcaaagggt ggattccatc tcggcaaaac gcagcgtaaa gtcgcgactg atggtgttct
     9301 gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc tccgaatcat
     9361 cctgaaacaa ggcggctaac tgcgcattca ccgcatcttg ctgttgacgc agaaagtggt
     9421 tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc tgcgcctgat
     9481 tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc aattgttcac
     9541 aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat
     9601 tcaacagttt atgcgcagca gatcgcagag catacaatga ggcgttagcg taagcggcat
     9661 gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat
     9721 gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat
     9781 gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttaccg ttgttggcta
     9841 actcatagcg cgcccgattc gccaggtagc cccacatcga cgacaccggg aatatctgct
     9901 gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc tggtcggcgt
     9961 cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc ggcaccgatt
    10021 gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc agttgcgtat
    10081 aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca
    10141 gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac tgaccgggat
    10201 aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg
    10261 cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc actaaatcat
    10321 tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt tcgaaagcga
    10381 cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg
    10441 tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt aaacaatcga
    10501 ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta tggcgaataa
    10561 gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta ccaacaatgg
    10621 cattaatggt ggttgatttc cctgctttca tggtaccgac aattgccagc accatttcca
    10681 gtcgggaaat tttacacaac tcattattca gcatcgcgtg acgttcggcg atattaggct
    10741 gactccaggg taaagccagt tgtggcgcgt cgtctccggg tacagagaga ggcatttttt
    10801 ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc tgacttaact
    10861 catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg ttacttttat
    10921 ttagccagat tgtttttgag ttctgttttc ggcttttata attactgcaa gaaataattt
    10981 tatatttagt gtgttgtttt ttatcagaat aaataacgtc ttctgatgcg tttaaaacgt
    11041 cagaaagata aaaatatcat gtgaataaa
//
LOCUS       DAEBXD010000118        10901 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEBXD010000118 DAEBXD010000000
VERSION     DAEBXD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.118"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="KJL94_003395"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="KJL94_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBD4411624.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KJL94_003396"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KJL94_003396"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBD4411625.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="KJL94_003397"
     CDS             complement(2271..3686)
                     /locus_tag="KJL94_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411626.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KJL94_003398"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KJL94_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBD4411627.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KJL94_003399"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KJL94_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KJL94_003400"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KJL94_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBD4411628.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DAEBXD010000119        10746 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEBXD010000119 DAEBXD010000000
VERSION     DAEBXD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.119"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(132..1481)
                     /gene="lysC"
                     /locus_tag="KJL94_003401"
     CDS             complement(132..1481)
                     /gene="lysC"
                     /locus_tag="KJL94_003401"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBD4411629.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2006..3655
                     /gene="pgi"
                     /locus_tag="KJL94_003402"
     CDS             2006..3655
                     /gene="pgi"
                     /locus_tag="KJL94_003402"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBD4411630.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGNHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4117..4359
                     /locus_tag="KJL94_003403"
     CDS             4117..4359
                     /locus_tag="KJL94_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411631.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4473..5111
                     /locus_tag="KJL94_003404"
     CDS             4473..5111
                     /locus_tag="KJL94_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD4411632.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5108..5845
                     /locus_tag="KJL94_003405"
     CDS             5108..5845
                     /locus_tag="KJL94_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD4411633.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5845..7941
                     /locus_tag="KJL94_003406"
     CDS             5845..7941
                     /locus_tag="KJL94_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD4411634.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7988..8266)
                     /locus_tag="KJL94_003407"
     CDS             complement(7988..8266)
                     /locus_tag="KJL94_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411635.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8267..8410
                     /locus_tag="KJL94_003408"
     CDS             8267..8410
                     /locus_tag="KJL94_003408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411636.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8536..8946
                     /gene="psiE"
                     /locus_tag="KJL94_003409"
     CDS             8536..8946
                     /gene="psiE"
                     /locus_tag="KJL94_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBD4411637.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="KJL94_003410"
     CDS             complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="KJL94_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBD4411638.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2728 a         2740 c         2777 g         2501 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcatag tgacaagaaa atcaatacgg cccgaaatat agcttccagg
      121 ccatacagta tttactcaaa caaattacta tgcagttttt gcaccacctg ctcggcatct
      181 tcgccgggca ccaggaagca caggttatgg ctggatgcgc cgtaacaaat catgcgaatg
      241 ttgaacggtt ccagtacgcc gaatacctct ttgccaacgc cgcaggcttt tgacaggtca
      301 ttgccaatca acgcgaccag cgccagacct tcttccacct ccacccggca cagtgcggaa
      361 agctccatca gcagagattg cgtcagcaac gtatcgccag tggaggttga accggtggta
      421 tcaagggtta atgccacgct cacttctgac gtggtgatta agtctaccga aatattatgc
      481 cgcgcgagga tgccgaaaac ttccgcgagg aagccgcgag aatgcagcat attcaggctg
      541 tgcaaagtga gcagagtctg attgcgacga agcgccagcg cgcggaacag cggcggattt
      601 tcagttttat tgcacaccag cgtaccacct gcgcgtgggt ctttgctgga gccgacaaag
      661 accgggatat cgctgcgtac tgcgggtagc aacgttgccg gatgcagtac ttttgcacca
      721 aaagttgcca tctctgccgc ttcggcaaac gcgatttcat caatgcgttt tgctgcggaa
      781 actacgcgtg gatcggtggt gtagatgccc gggacgtcgg tccagatatc aacacgagat
      841 gcgtgtaaag cctccgccag caaggctgcc gtataatcgc tgcctccacg gccaagcgtc
      901 gttgtacgac ctttattttc gctaccgata aatccctggg tgatcactaa gccttcattg
      961 aggcgtggga gcagctgcag cgcggccagt tccgccagcg cggctacatc tggctctgca
     1021 cgaccaaatc ggtcgttggt acgcatcact ttacgtacat caaaccactg tgcctgaaca
     1081 tcgcgttcgc gcaggatttc gacaaacagc agggtcgaca tcagctcgcc gtggctgacc
     1141 agctcatctg tcagcgccgg agacgttgcc agcgccgccg cttctgccag aacagtaatg
     1201 ttctccagca gacgttcaat ctcttcacgg ataacgttcg ggtaacgcag acgttccaga
     1261 atggcaaact ggatgttgcg aatagcgtcg agtttttcga atcgctcgcc aagttccagc
     1321 ccttccgcta aagcgaccag cagattagtg ataccagcag aagccgagag gactactaaa
     1381 cgcacgttgg catcagaaag cacaatatca gcgctgcggt tcatggcgtc aaaatcagct
     1441 acgctggtac cgccaaattt ggagacaaca atttcagaca taactacctc gtgtcagggg
     1501 atccattttc agccttggca caagggaaga gcggaagacg ggtgggcgca gagcgatact
     1561 tcgctactat tttcacccag aagtgctcca ccacttgcga aacgcccgac tgcgaacgct
     1621 tctggtgaca acccagggga ttcagcccct gtagccgatg atgaacgtgg ccagccgttc
     1681 aatcacctcg gcgatgcacc ccctcaggtg ttatcacagg actggctcct ccaacaccgt
     1741 tacttgggca acgcgcctct tctggcctgc gctagcgcag gtagtacatt tataaataaa
     1801 gggtgagcgg ggcggttgtc aacgacgggg tcatgcggat ttttcatcca ctcctggcgg
     1861 tcagtagttc agctaataaa tgcttcactg cgctaagggt ttacactcaa cattacacta
     1921 acggcactaa aaccatcaca tttttctgtg actggcgcta caatcttcca aagtcacaat
     1981 tctcaaaatc agaagagtat tgctaatgaa aaacatcaat ccaacgcaga ccgctgcctg
     2041 gcaggcacta cagaaacact tcgatgaaat gaaagacgtt acgatcgccg atctttttgc
     2101 taaagacggc gatcgttttt ctaagttctc cgcaaccttc gacgatcaga tgctggtgga
     2161 ttactccaaa aaccgcatca ctgaagagac gctggcgaaa ttacaggatc tggcgaaaga
     2221 gtgcgatctg gcgggcgcga ttaagtcgat gttctctggc gagaagatca accgcactga
     2281 aaaccgcgcc gtgctgcacg tagcgctgcg taatcgtagc aataccccga ttctggttga
     2341 tggcaaagat gttatgccag aagtcaacgc ggtgctggag aagatgaaaa ccttctcgga
     2401 agcgattatc tccggtgagt ggaagggtta taccggcaaa gcaatcactg acgtagtgaa
     2461 catcgggatc ggcggttctg acctcggccc gtacatggtg accgaagcac tgcgtccgta
     2521 caaaaaccac ctgaacatgc actttgtttc taacgtcgat gggaatcaca tctcggaagt
     2581 gctgaaaaaa gtaaacccgg aaaccacgct gttcctggta gcatctaaaa ccttcaccac
     2641 tcaggaaacc atgaccaacg cccatagcgc gcgtgactgg ttcctgaaag cggcaggtga
     2701 tgaaaaacac gttgcgaaac attttgcggc gctctcaacc aatgccaaag ccgttggcga
     2761 gtttggtatt gatactgcca acatgttcga gttctgggac tgggtcggcg gccgttactc
     2821 tttgtggtca gcgattggcc tgtcgattgt tctctccatc ggctttgata acttcgttga
     2881 actgctttct ggcgcacacg caatggacaa gcatttctcc accacgcctg ccgagaaaaa
     2941 cctgcctgtc ctgctggcgc tgattggcat ctggtacaac aatttctttg gtgcggaaac
     3001 tgaagcgatt ctgccgtatg accagtatat gcaccgtttc gcggcgtact tccagcaggg
     3061 caatatggag tccaacggta agtatgttga ccgtaacggt aacgttgtgg attaccagac
     3121 tggcccgatt atctggggtg aaccaggcac taacggtcag cacgcgttct accagctgat
     3181 ccaccaggga accaaaatgg taccgtgcga tttcatcgcc ccggctatca cccataaccc
     3241 gctctctgat caccaccaga aactgctgtc taacttcttc gcccagaccg aagcgctggc
     3301 gtttggtaaa tcccgcgaag tggttgagca ggaatatcgc gatcagggta aagatccggc
     3361 aacgcttgac tacgtggtgc cgttcaaagt gttcgaaggt aaccgcccga ccaactccat
     3421 cctgctgcgc gaaatcactc cgttcagcct gggtgcgttg attgcgctgt atgagcacaa
     3481 aatctttact cagggcgtga tcctgaacat cttcaccttc gaccagtggg gcgtggagct
     3541 gggtaaacaa ctggcaaacc gtattctgcc agagctgaaa gatgataaag aaatcagcag
     3601 ccacgatagc tcgaccaatg gtctgattaa ccgctataaa gcgtggcgcg gttaatcatc
     3661 atgcgtgcct gatgccgata tgtaggccgg ataaggcgtt tacgccgcat ccggcaaccg
     3721 gagcctgatg ccaatatcga cattaatgca tagtatgagg agttaaataa ccttaaaaac
     3781 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
     3841 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
     3901 tacgcagatt aattactttt gtttttattt taagcttatg atttttattg ttatttaaat
     3961 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
     4021 cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
     4081 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
     4141 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
     4201 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
     4261 accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat
     4321 accgggacca tcacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
     4381 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
     4441 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
     4501 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
     4561 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
     4621 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
     4681 aagacggaca acaaaaatgg gtcactcagg atcaagccac tcttgtcaca caacatggtc
     4741 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     4801 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     4861 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     4921 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     4981 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     5041 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     5101 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     5161 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     5221 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     5281 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     5341 gctgacacgg ctggcagaac agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     5401 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     5461 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     5521 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     5581 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     5641 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     5701 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     5761 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     5821 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     5881 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     5941 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     6001 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     6061 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     6121 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     6181 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     6241 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     6301 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     6361 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     6421 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     6481 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     6541 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     6601 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     6661 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     6721 cagccgcagg atgccatttt gcagcattcg gtagtggcga atcagttaac gttgctgaaa
     6781 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     6841 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     6901 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     6961 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     7021 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     7081 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     7141 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     7201 gatttgtggc tgacggacca tctactgacc accggcagcc tgtttgcaaa tcttgccaac
     7261 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     7321 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     7381 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     7441 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     7501 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     7561 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     7621 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     7681 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     7741 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     7801 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     7861 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     7921 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     7981 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     8041 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     8101 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     8161 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag
     8221 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     8281 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     8341 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     8401 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     8461 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     8521 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     8581 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     8641 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     8701 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     8761 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     8821 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     8881 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     8941 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     9001 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     9061 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     9121 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     9181 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     9241 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     9301 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     9361 ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagtg
     9421 cgccgataat ttgcagtggc ttacgaccaa atttatccac agtcataatt gccagtacgg
     9481 taaaggtgag gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg
     9541 cccccagcgt tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt
     9601 gctggaagat ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac
     9661 gaccaccggt tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg
     9721 caagcgtgtt gcccataatt ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg
     9781 acatcagcca gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag
     9841 ggatacattc cgaggcaaac atataacgcc tgccgtcagt attcagccag ctggcatcac
     9901 cggaacgggc aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa
     9961 actggttaaa tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca
    10021 ttggtgaaag cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa
    10081 attccgggac ataacctgcc agataaatag gtactgtgtt gtccgggttt atagaggtaa
    10141 aaccaagttc tggccaggca gaacctacac cagaaataaa aaacaggaca gcagaaatct
    10201 taagtgaatc acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga
    10261 tgcaaccaat cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac
    10321 ttaagttttg tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg
    10381 cggtgtcgta gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat
    10441 aactggaatt atactgggta ttcattcaga cctgccttag accattctga tcttttatca
    10501 gtgcattgat gttggaattg tcaggcagtt aaggcaatta tcatttttta caatgcgttt
    10561 acgtaatgtc ttttctgtga tcttaattgt gataattatc aaaaattgat gaaaacaata
    10621 ctattgccgt gactcagagc acgaaagaga attatcgtaa gtgggaaaac aaataacgta
    10681 aaaataataa gttctatgat gggaatatta accggcgaac gattcagatt gcagacgaaa
    10741 gaaaaa
//
LOCUS       DAEBXD010000120        10520 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEBXD010000120 DAEBXD010000000
VERSION     DAEBXD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.120"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(85..357)
                     /locus_tag="KJL94_003411"
                     /pseudo
     CDS             complement(85..357)
                     /locus_tag="KJL94_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(451..1013)
                     /locus_tag="KJL94_003412"
                     /pseudo
     CDS             complement(451..1013)
                     /locus_tag="KJL94_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1217..1423)
                     /locus_tag="KJL94_003413"
     CDS             complement(1217..1423)
                     /locus_tag="KJL94_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBD4411639.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1530..2093)
                     /locus_tag="KJL94_003414"
     CDS             complement(1530..2093)
                     /locus_tag="KJL94_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBD4411640.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3468..5303)
                     /locus_tag="KJL94_003415"
     CDS             complement(3468..5303)
                     /locus_tag="KJL94_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411641.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5404..5691
                     /locus_tag="KJL94_003416"
     CDS             5404..5691
                     /locus_tag="KJL94_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4411642.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5663..7192
                     /locus_tag="KJL94_003417"
     CDS             5663..7192
                     /locus_tag="KJL94_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBD4411643.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7362..8579)
                     /locus_tag="KJL94_003418"
     CDS             complement(7362..8579)
                     /locus_tag="KJL94_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4411644.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8946..9932)
                     /locus_tag="KJL94_003419"
     CDS             complement(8946..9932)
                     /locus_tag="KJL94_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411645.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9929..10457)
                     /locus_tag="KJL94_003420"
                     /pseudo
     CDS             complement(9929..10457)
                     /locus_tag="KJL94_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3273 a         2410 c         1984 g         2853 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcacttaca ccagacatcg ttattaagta gcagcaccat
      121 aagataagga gtggcatcgt tagccacagc ttccctactc cagaggtaat ataaaggggt
      181 gggctcgact gatttatctt tacgtcgctt acactgcaaa tgttcagaaa ttagtctatg
      241 cagctcacca gtaaaatcag caatcagaga aggaattgcc ttattcatac cagggcaagg
      301 tattaattta aactgtaata atttaatttt aggatgtgta gcagcagccc gatacagagt
      361 tgcaaggaca cacttttgcc agagggcgtt acaggaaagc ttaatgtttg attctgtata
      421 cataataaat caccttatag ttacaatagg tcaaaaaccg ctgtagccaa agttacgctg
      481 gcctgatgct ttagtgtcga gcttcgtcaa ataatccaga cgtaccaata agcgctggta
      541 ctgctctggg aaattaggat cgttaatatc caggatgtaa cgtccattag cagggaaatg
      601 aactaatccc tggtgatctt ccagttccaa ccctaaggca ctgtaccagg ctccggtaat
      661 taaccctctc agggtgtcag agcgctcata gtttcccaga tggtaatagg cgtctttgtt
      721 aaaaaaaggc agacgtgata atgacattta aaggactgcg tatattcttt cgcccagata
      781 atacaaaccg acgttgcgta ggtgcgtttg ccttgttgtt ttttacggtg ttgatctgca
      841 ttaattttcg cattcagcga gttacggaaa cgagatatct ctccggggat caggttaggg
      901 aaacaggtaa tgatatcccc gttgtcaatc acaggagggt aatggagatc cccccgtatt
      961 gctgttattc tggaagtgtt tttaatattc gtattgatag ttctcagaga tatatcacac
     1021 tctagatatt ttaattaagt atcagttcga tatcggcata ttgtacacta cctcaggtag
     1081 atgatgagat cagatacagg gatcaactga cggtgagcgc agtccccaca ttttatccag
     1141 ggcttactgc atattcccaa cagccattcg ttagccaggt agacgaatac cctgatttgc
     1201 cgctggtttt gctttcctat cgaagggccc atacatcatc acgcccacga aacaaccgac
     1261 gaaataacgc aactttctca tccgtggata atacgaaaac gcactgcaca gggctctgcg
     1321 gtttacgtcg ccattcaatc ccatgagctt caagtaatga gaccagccgg gtattttctg
     1381 cccgcagtac tgctagttca tcgctgtcag atttataaac catatgcgtt atcacttgta
     1441 cctgtctccc ggcggcacca cgactgaaat tcgtgtgaag gcaaatccac gcgataactt
     1501 acgatagttc ggacataagc ccccataatt cagttatgtc gtggtcgacc ccggttactt
     1561 ttttcgggaa gcctgctacg gcccagaata ataccgtgtt ctttctgatc ctgtttagcc
     1621 agatagctaa gggcgtaacg taagtcgtct accgcagatt tatcactata gtgaatcacg
     1681 tgatcgatac gcaccgggta tttgtcttta gcccggcaca gatgaaaata accctcccct
     1741 tccgtgatcc gcctccagat atctcccagt tgcctggata tctgataaga gttcttgtga
     1801 cgttgtccat ccaggtagcc gataaaatga atatgaagcc ctttgtctgg tgtatattcc
     1861 attacccagt aatatcccgc caacatcgtc tgggtttcgc taagtaaacg gtatatttcc
     1921 atacacatac tgtgcttaca ggaatgcccg aaactgggcg tgtctttcct gtaagcaaaa
     1981 tcaattctga aaggtaacag tttagaaaaa cgttgaaaca taccatccat gtgttcattc
     2041 acgtctttca gaatcatgaa atccattttg taattgggat tggcattata cattttatag
     2101 tccttacttt ataaaactca caggaagaga gaattacgta aatactgaga gtagagaaac
     2161 gccgtaccac ggcattaata aacgaagtaa accagcagca cagacaacct gataaaactg
     2221 ttttgtatta ccgcattact gtgatgaggt aatacttcag aatacagtga gactctctta
     2281 aactgagaac cagtacgaat tacaatggaa aaacacacct catagaattg agtttcagga
     2341 agcaccacta aattctttac aggaagtaat ctgacattca caaaactcag tatcggctag
     2401 ctacaggcaa catcgctatt aatattgtcg aaccattaag taaagccgag agagaccacg
     2461 ggtatttaat cattagcata aaagaataat ggtatacaga acatacccac acaccataag
     2521 aagattgttc catacagaaa agatcatatt tacctatacc tataaactgt aacggagtca
     2581 taacacgctc taatagtgtt ccgatacgta accagtgtta agtacgtaca ggacaacatt
     2641 acacacataa ttcaccatac agcttccccc atatgcttca ctgcgtagtg tatggagata
     2701 tatgcattac acacatacag aatacttagc ataaagagga gtgacattac cacgtaaaga
     2761 gataagttat acaacaaaca tacaaacaat acggcacaga cacacaatac cataactcca
     2821 actaataata ctaattaacc ttaattattt atggtatatg aaataaatgt gattctcccg
     2881 gaactaacta acctaaccag acagatgatc actgtataaa aacttcagtt aatactcatt
     2941 tgttttgtcg actcagtata taatgagtga ccagtaatca ggataatcac acttacataa
     3001 cctgtaaaaa agaacaagaa gaataagcca ctaacataat gcaaaatggc atctgaatcg
     3061 atcgcagttt ggcattcgaa tcgatcatga tttggcccaa tccgtgatcg aatagagcgg
     3121 aatacgcaac ataattcacc agacaagagc ttacaccgaa ttacagatta tgtattaatg
     3181 tcaaaactgg catattttac agatacaatg aacatgcaac ccatcaatct aataggggcg
     3241 gtatgtacat ctgcccatga atgggatggc gctaagaata caaaacaatt agacatagat
     3301 cacaaaaaca caccattcta attgaaaaac aaaacaaaca aataaatcat actattatag
     3361 aattttctct tgccagacat agataatacc cctgctaatt ttgctaatgg ttagcaaaat
     3421 tagcagggta tataaagatt attgacccaa aacacactca gatgaattca cgacagctca
     3481 ggagggttat tcctgatgtc atcttcacaa ataatggtta acgcatcata tatcattctg
     3541 cgtctgttcc tgttttcggc cttatcgcca tacaacatca tcacattttt aatttcatcg
     3601 accggtgatt tcacatagtg aactaataca ccaagcttct caacacattg cagtaaagta
     3661 tatttcttac tgcctgtgaa aagccctatc atagtttctt tacgttcgcg actggataat
     3721 actttgtttt ccataatgac ttctgccagc gtcttaccat cataaacata ctctttatct
     3781 atcagaatat ttatcttatc cagcaccttc tctacgtcat caagcaaacc tgtaatatat
     3841 gggggcgcaa tatatggttt cgtactgatc ttttcaagca tacaattata catcttaata
     3901 acacgcatca gggtaaaatt gttcgcatca tactgtattt caccctcctt atatacagga
     3961 tcaataagtg gtattttttc aataaatgca tcctgatagg cttttacatg gtcaagccaa
     4021 taaaacaacg agccatactt tatatttttt ctatttactt taacctccag tgctgtccga
     4081 agcaacgata atgcccccct gacatatcct gaaggtaatt caccaaacaa tccttttctt
     4141 tccatgtcat cataatgctg cagcacatca ttaattttat ttttccttat cagacaaatg
     4201 tacaccgcaa gtgtttcgta gcagctggtc acatgaacag cctcattctg caactgccca
     4261 aaatagctta taatcttttt cagatatgcg tcatcacaac cattcttatc attaatatta
     4321 aatacaagat ctttcagacg ggaaatatat tcctgagatt tatcatcaag tgaaacacta
     4381 taacgagaca atgttccttc tgcactgttt tgaaacttat aaaacatttc cagtaatttt
     4441 tttgcataag gcgttgtatc aaagctggta aagtcataaa gatattgcgc agcgtaacca
     4501 tacaaaccat gaatccagag tccttctctc accaacactc tgagataata tgcaaggctt
     4561 tgaagtatct tcgtctttcg ttgtatatca agtaagacac ttaatgaata aaatgcatta
     4621 tagaatttat caatattcct gagcactttc tccagttcat ccttatccag cttcttctcc
     4681 cggtaatcct ttttatattc atttttgata gccatgatat attgttgcat caggctcttg
     4741 cttttcccgt tatccttccg tatagttcct gtatatttcc ccagagcata cagctttcca
     4801 ttttcatgaa aatcttcctt gttgaatccc agttcagcca gatattcccg aagataacgt
     4861 ttaacagcag cttcatcgct ttctgtatca gcgtgatcaa gacaaggaca atacgatttc
     4921 tgtaaaaaat tataaatatg ataataaaaa gcagagtcat tctctggtct gtatgtatta
     4981 tacatatcta caacaagaaa aataacccca aaagtgcgca gacaatagct gcgaaggaat
     5041 tcaggtttta gtgtggaatg cactggataa gaattcatca tgtaataaaa atgttcatat
     5101 cgggcatata tgatccgccg ttgctcctga ctgaacaccc cttcagcatc aataatacga
     5161 ctgatctcat tataacgttg atcccagaat ttttgatgca tttcaaaatt ggtactcttc
     5221 cgcttgtgtg ccggattctc gccctcctta aacaacccca aaacagtaca aatatcatac
     5281 aacatatttg taaatgtgcg catatgctgt gctctccggt tggtttgcat tttatcgcag
     5341 aggacattct gagcttaacg tatcaaactg acacaacgac atgcaatgca tatggagaac
     5401 aatatgtcaa atcaaaccgg acagctttac acgccttcac gcatcctgcg tatggctgaa
     5461 cttacccgac tgctgggtat atcaagatct actatttacg tgaaaataaa tccggcctcg
     5521 aaataccacg accctacctt tcccaaacca ataaggcttg gtagtgcatc cgtaggctgg
     5581 cgggcatctg ccatcgatga gtggctgatg ttacatacgg caccagcctg gtctgaacct
     5641 gaaaacaaac taaaggaact taatgatgac gacaaaacta tcactccctg aacttgacac
     5701 aactaaactg cctgacaggt gccaggcatt acttgatgaa atgcatgaag aaacaggaat
     5761 acgccgtgaa atacttctgt ctgttatgct gacggtcatg gctgcctcgg tccaggatac
     5821 gcatgaagtt gaactttccg gagggcaacg taccagcctt cagatattta tgtgtctttc
     5881 atcagcatca ggcagtggaa aaacctctgc ctgcgcaaaa ttaatcgctc ctgttcatga
     5941 aacagaagaa gaactacatc aggcatatat cgacgataaa aaaaactatg accgaatgat
     6001 ggaaatgtgg acaacagata aaaaaatcct ggagcggaga tataaaaagg aaattgaaag
     6061 atccccggaa aatgctgctg ctgcacgagc agcacttgaa aagtgtatag caaataaacc
     6121 cgaacctccc gtacaacagg ttctcatcgt gaatgacgcg acaccagaag ggatagcttt
     6181 gaaactcagc cagtcaccct ctcttctgtt gctgtctgat gaaggaggta ctattctgga
     6241 taagcgtttc gaacgcaaat cggcactgta caacacatta tggagtgggc aaccagtcaa
     6301 cgttgaacgg gcatccagac ccgggttccg gctgaatgat gtccgtctca ctatacttat
     6361 actgactcaa ccggtaatat ttaataagtt ttttactctc actggcgatc agatccgggg
     6421 caatggcttt ctggcccggt tgctattttg cgaacccgga gcaattaaaa taatgacgac
     6481 agaaccgcat actgccactc ctgttgtgac gcaacaatgt gcaagctgtt ttcgtaagaa
     6541 aagttttggc agtcagatca gagactctct gagagcatcc agagaaagac gtgcaaaaag
     6601 taaagagcgc atctgcatga cattatcaaa tgcagcatcc cgcgcactgg agcgctttca
     6661 caaagaaaat atggatactg tcagacagaa tcctcatatg ggtacatttg aagacattat
     6721 tgtcagaaaa agagagcagg ttgtcaggat agcagcactt ctggaactgg agaaagatcc
     6781 agactcgaca gtaataacgc tggaaagtac taatagcgcc atttatctca ttgaatttta
     6841 tttcaagcat cttatatata aacttgaatc ccttcgggaa atatccccgg ctgagaaact
     6901 tgataaatgg cttcagaaaa gaattatcac aacagcaggt tacatattcc agaaaagtta
     6961 tattttacaa tatgccccat atgctctcag gaaaaaatgt gttctggatg aagcacttga
     7021 catactggcg gaacaaagga aaataaggat tgatgataac ctggttgttt atataggaaa
     7081 cacgattaca ccatccgaac tggcaaaaaa actaaatatt cctgcattcg atgccggagt
     7141 tttcatttgt gatcatcaaa acatactgaa gtatcaccga aacagactat agatactatc
     7201 ggacaaaaaa taaattatca caagtgtatt attcgttata ataatgaata atcgttatct
     7261 gagtgcaatg ccaggttttc attacgaact ggcattgcat ttatttttca aaaaagaaag
     7321 cagtaaccat ttggctactc tgagtatgcc agtccatgaa actaagcgaa ttccttagtg
     7381 tttttagcaa gttgagacat cacataatcc gcccatgcct gcatcatcgg tacacgctgc
     7441 tccagtagat cagtacgatg atatgccgcc tcaaccttat ttttcagcgt atgagcgagc
     7501 gccctttccg ccagatcccg cgaatacccc tgttcactac accagtccct gaatgttgag
     7561 cgaaaaccat gtgccgtggc aactcgcccc ggaatgtcac tgacggcttt ctttttacgc
     7621 agaaaacttg tcaacaccat atcggaaagg atctgctgct ttctgggtga agggaacacc
     7681 agttcatcat gcaggccacg tatattttcc agaatgtaaa tagcctgccg ggataaagga
     7741 acacgatgct gtagcctagc tttcattctt tctgcaggta tagtccatac ccgcttatga
     7801 aaatcaattt cagcccagcg cattcccctg gcttcgcccg agcgagttgc tgtaagtatc
     7861 accattaata acagtgcgcg ggtaacatta taaggttcat cactatacac actggtcgcc
     7921 acaaaaagcg gtaactgcct ccagggcatt gcgggttggt gttcatcacg tcctcttgtc
     7981 tgctgaggaa gcaaatggtc aaccacatca acaggatttg ccacacaaaa accgtgcgcc
     8041 catccccact gcataacaac atgaatgcgc tgtttaaccc ggcttgccgt ttctgacaag
     8101 gttaaccaga ctggacgcag tgtttctgcc acgtccgcag ccgtaatcga atccagcgtt
     8161 tttgctccca gttgaggaaa cgcgtaattc tcaagcgtcg ataaccactg ccttacatgc
     8221 tttggattct cccatccagg agacagttct gcatgtacac gcctggctgc atcggcaaat
     8281 gttgggataa cgactttctc agattcagcc tttttaatct ccagaggatc atcacctgca
     8341 gcaagttgct ctcgcattat ccgggcagta cgtgcagctt cagcaatact gacctctggg
     8401 taagttccca atccagcatt acgtcttttt tgtgtcaccg gacttacata acgaaaaacc
     8461 catttccccc gcccctttac tgaagaagga tgaagggtca gtccggtaat tcccccatgg
     8521 ggcaatggtt tgtcatcagg tttgatatgt cttgctttcg tatccgtcaa tactgccata
     8581 cgctaattcc cgtcactctg gtatgccatc cagtatgcca ttagtccctc gcttcactaa
     8641 gaatacatta gataacatcg gacaacaaaa agtgtaatac attgtttata aagaaattat
     8701 ataacacccc tggattaagt cagatttatt tcaggcggtc cccctcaccg ccatatttaa
     8761 agaagagcgc gtacgaaagt acgggctttt ttttcgtata ttgtacacac cgggtgcccc
     8821 cgcccacgtc tgttagggcg aggggaaatt gtgcacatca actgtctggt tactcagtca
     8881 acaatcaaaa tgcgtaagat agtacagaac atatatgcgt tatctggtca cttcctgtaa
     8941 cttcctcact tattaaaatg cccccaacgg aaatacatgt cacatgccat cataaaatcg
     9001 ttatgccaga ggaatcgaca atggaaaaaa tagtaaatta agcacggtac atagcagagt
     9061 aaaaaatagg cttcaaacca aatccgccat tccacaagcg ttgccaatac cgacaccagc
     9121 gtaatccaca tcatttcaat aacccgcaat atgaataacc gaagccaaag cggcttggca
     9181 aaaaaggtcg acaacgtatt ttcactggag aaaaacagat cgatttcctg caaaaaaaac
     9241 ctgcccagaa aagcgggccg cttggagaag cgcagaaaaa acagattaat cgccaacact
     9301 acgccacaaa gaatgagcaa tgcaattatc gatttaaacg ttaaccactg cccctctgtc
     9361 acaaaagaat cactgttacg caacgggctg atagtaagca ggttaacaac cgttgagatg
     9421 cacaccgaaa gcagcacata gcgagtcaga cttttatatt ttactttttt cgctactgaa
     9481 atagcgacct gcttgacata actggtgcgg atcatccgaa acagcagaaa gatgaccaac
     9541 ggcccaagta acgaaatcag gaacagcgca tataacgagg gtagaagctg gtatagcccc
     9601 caactcattg cactcaacat gattaacgac agaaatcctg ccaccagagg ttgtgtaaga
     9661 aataattcta ttttgtggtt ctgctttttc ttaaaccacg aaaaatcgat attcttcgtg
     9721 acgcatttat aagcccagta cgccttgaga tcaaaaataa aattatacag caccagggcc
     9781 agaatcccac taactacacc aatatcctct gatggaaaag taaaccctgc atttttccaa
     9841 taaaagccta tcagaataag tccaatagca taaatgtata tcagatttcg tttatcctga
     9901 aagcgaataa aagttaaata atcaggaatc atttgaatac ctttgcatca ctaaatgctt
     9961 tacgacaatg cataactcgt tgctgatcca cgccagcaaa atcatttaac atattcccca
    10021 aatagggctt attacttatt tcaaggaaag ctaccggggt tggcatattc agaatatgta
    10081 tcgagagatt cagtccattt caccgtatga gttaagtgta atgagagatt ttctttgata
    10141 attgacggag cgacctcgct ttttgctgtt acgttcatca gaacctgatg ctctggcgaa
    10201 gcgatatcca gtccggcaag ataatcgcgc attgcctgaa cgccgtcttc catcaaacgc
    10261 gtgtgccagg caccgctcac accgagttta accggttcgt atccagcggc catcagcagc
    10321 gtggtaaatt cattcaacgc agcctgcgtt cccccaataa cctgctggcg cggcgtgtta
    10381 tcacagctaa tatccagcgc aatgcctgat tccgtaatca tcgtctgcag ttgttcgcgg
    10441 ttgatgcctt taaccgcctg atcttaccca gcaatagtgg acacgcggct aagtgagtaa
    10501 actctcagtc agaggtgact
//
LOCUS       DAEBXD010000121        10118 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEBXD010000121 DAEBXD010000000
VERSION     DAEBXD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.121"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            134..265
                     /locus_tag="KJL94_003421"
     CDS             134..265
                     /locus_tag="KJL94_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBD4411646.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            525..1520
                     /gene="pgl"
                     /locus_tag="KJL94_003422"
     CDS             525..1520
                     /gene="pgl"
                     /locus_tag="KJL94_003422"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBD4411647.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1561..2514)
                     /locus_tag="KJL94_003423"
                     /pseudo
     CDS             complement(1561..2514)
                     /locus_tag="KJL94_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2698..3750
                     /locus_tag="KJL94_003424"
     CDS             2698..3750
                     /locus_tag="KJL94_003424"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBD4411648.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3826..5259
                     /locus_tag="KJL94_003425"
     CDS             3826..5259
                     /locus_tag="KJL94_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBD4411649.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5442..7622
                     /locus_tag="KJL94_003426"
     CDS             5442..7622
                     /locus_tag="KJL94_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBD4411650.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7763..9046)
                     /locus_tag="KJL94_003427"
     CDS             complement(7763..9046)
                     /locus_tag="KJL94_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBD4411651.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9181..10062)
                     /locus_tag="KJL94_003428"
     CDS             complement(9181..10062)
                     /locus_tag="KJL94_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4411652.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2457 a         2364 c         2553 g         2744 t
ORIGIN      
        1 tcaatgtact gtttttttct ttaaaaaaac agtacattct attttataat gactgtgtat
       61 tcccatggaa ggagatcacc caattctccc ccccgacttt tgacgcagtg tcttgttaac
      121 agggggattg accatgctta ttggctatgt acgcgtatca acaaatgacc agaataccga
      181 tctacaacgt aatgcgttta tttgtgccgg aagtgagttg attgttgcgc gaatgggaag
      241 ttggccagaa gttgttatgg catagtgttt acggtcgttg atacaggtaa acaaaagcgg
      301 ggagagtcat actccctgaa tagtcgcgaa aatatctgtg gcattgtcaa attcgtgtcg
      361 gtgactaata ttttagtaat atgccattcg aaatggagtg ctaacttatt gatttaattg
      421 atgtgcgata ttgtatttta tcgtgttttg ttgtctgttt tttatccagt aagtgttgtt
      481 atatgtatga aagataaggg atttatcatt tttgaggtgt gttcatgaag caaacagttt
      541 atatcgccag ccctgagagc cagcaaattc acgtctggaa cctgaatcat gaaggcgcac
      601 tgacgctgac acaggttgtc gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg
      661 acaaacgtta tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg tatcgtattg
      721 ccccggacga tggcgcactg acctttgccg cagagtctgt gctgccgggt agtccgacgc
      781 atatttccac cgatcaccag gggcagtttg tctttgtagg ttcttacaat gcgggtaacg
      841 tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgta gtcgaggggc
      901 tggacggttg ccattccgcc aatatctcac cggacaaccg tacgctgtgg gttccggcat
      961 taaagcagga tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg gtggcgcagg
     1021 accctgcgga agtgactacc gttgaagggg ccggcccgcg tcatatggta ttccatccaa
     1081 acgaacaata tgcgtattgc gtcaatgagt taaacagctc agtggatgtc tgggaactga
     1141 aagatccgca cggcaatatc gaatgtgtcc agacgctgga tatgatgccg gaaaacttct
     1201 ccgacacccg ttgggcggct gatattcata tcaccccgga tggtcgccat ttatacgcct
     1261 gcgaccgtac cgccagtctg attaccgttt tcagcgtttc ggaagatggc agcgtgttga
     1321 gtaaagaagg cttccagcca acggaaaccc agccgcgcgg cttcaatgtt gattacagcg
     1381 gcaagtatct gattgccgct gggcaaaaat ctcaccacat ctcggtatac gaaattgttg
     1441 gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt cgggcaggga ccaatgtggg
     1501 tggtggttaa cgcacactaa ccgctgattt aaccggcgca gtctctcctg cgccggtgta
     1561 ttaacttatc tcctgtaacg catgtctctg gcgttcgacg atattggtcc acaaattgtc
     1621 tttatcgtca gtccacaaat taatcagcaa ggaaaaaaag cgttcagctg ccggagaaag
     1681 cacggcatct ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt ccaccagtgg
     1741 gataccaata atcgaagaat agggcgcatg gggcattgcc agaccaggaa gcgccgaaat
     1801 ccccagtcct gcctccacta gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca
     1861 gtccagcttc cagggcttgt cggctagctg ttgttctatc aacaatcggt tgccgctgga
     1921 ggaacgtacg ccaatcattt tgtaacccac taactcctgc cattctacaa gctacttttt
     1981 ggccaatgga tggtcacgcc gacaggccag cacgaatggc tcgttgacca gtggggtaaa
     2041 atcaatggat gaatttgtga cgttgttcat gtttatgcca aagtctgatt cgttacacag
     2101 caccgattcc atgcaattat tcgtgccttg ttccagaatc cgcactttaa tattgggata
     2161 cagctcatta aatttaccga ttgccagcgg taaaaaataa aatactgcgg tcggaataca
     2221 cgctaatgtc accatatcac gatgataagc attcatatca cgaatattaa aaagcgtttc
     2281 atcgaatttc tttattaatt cccgcgcctc cgggagcaac ctttttcctg cttttgttaa
     2341 cgtaaccttg cgagttgtac gctcaaatag ttgaatatgt aaatcttcct ccatcttttt
     2401 tattcttcgc gttaatgcag gttgcgtaat attgagtaat ttagcggcat tgttaaatga
     2461 actggactct gccagtatga caaatgcctt catacttgat aattcatgct tcattattac
     2521 tccggaaaat ggaagcgacg attttggggg gctggtcgtt aaaaatttta actgcattta
     2581 gccaacttaa attaatgaaa aaatgttatt aatcgttgag ctaaagtcat tagagatgct
     2641 ttgtccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg
     2701 aaaaaaatac cctgcgtgat gatgcgaggt ggaacctcga gggccgcgtt cctgttagcg
     2761 gaacatttac ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc
     2821 ggtaacgatc tggaaattga cggtattggc ggcggtaatc cactgaccag taaagtcgcc
     2881 attattagcc gttccagcga tccgcgtgct gatgtcgatt atctgtttgc tcaagttatc
     2941 gtccatgagc aacgtgtcga taccacgcct aactgcggca atatgctgtc tggcgtaggg
     3001 gcattcgcca ttgaaaatgg tttgattgca gcgacttcgc cagttacccg tgtacgtatc
     3061 cgcaacgtca atacgggtac gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc
     3121 gagtacgagg gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc
     3181 actttcctga atgccgctgg aacgaaaacc ggaaaagttt tcccgactga taatcagatt
     3241 gattattttg acgatgtccc ggtgacctgt atcgatatgg cgatgccagt cgtcattatt
     3301 ccggctgaat atctgggaaa aacaggttat gaattaccgg cggaactgga tgccgacaaa
     3361 gcattattag cccgcattga atctatccgg ctacaagcgg gtaaagcaat gggcttaggt
     3421 gatgtcagta atatggttat ccctaaacct gtgcttattt ctccagcgca gaaaggcggg
     3481 gcaattaatg tgcgttattt tatgccgcat tcttgccatc gcgcgctggc gataaccggt
     3541 gctattgcta tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct
     3601 tctgtaggat atggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta
     3661 agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg ttattcggac aaccagaaaa
     3721 atattttccg gtgaagttta tcttccctga aaaaattcgt tgtcaggata aggacaatca
     3781 ataaaggact tctgtatgag tcatacagaa agaacaggat tttaaatgaa taagaaatcg
     3841 ttatggaagc taattctgat attagcgatc ccatgtatta ttggttttat gccagctccg
     3901 gcaggattaa gcgaactggc gtgggtgctt tttggtattt acctggcggc cattgtgggt
     3961 ctggttatca agcctttccc ggaacctgtc gtactgttaa ttgccgttgc tgcctcaatg
     4021 gtggtggtcg gtaacttatc cgacggtgcg tttaagacca ccgccgtatt aagcggttac
     4081 tcttcaggta ctacctggct ggtgttctcg gcgtttacct taagcgccgc atttgtgacc
     4141 accggtttag gtaaacttat tgcctatctg ctgattggta aaatcggtaa caccatgctg
     4201 ggtctgggtt acgttacggt attcctcgat ctggtactgg ctccggcaac accgtctaac
     4261 accgcgcgtg cgggcggcat tgtgttaccg atcatcaaca gcgtggcgat ggctttgggg
     4321 tccgaaccgg aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc catttacatg
     4381 gtcaccaaaa ccaccagcta tatgttcttt accgcaatgg cggggaacat tctggcgctg
     4441 aaaatgatca acgacattct gcacctgcaa attagctggg gtggatgggc gctggccgcc
     4501 ggattgccgg gcatcattat gctgctggtc accccgctgg taatttacac catgtatcca
     4561 ccagaaatta aaaaggtgga taacaaaacc atcgctaaag cgggccttgc cgaactggga
     4621 ccgatgaaaa tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct actgggctgg
     4681 attttcagta agtctctggg ggttgatgaa tccactgtgg caatcgttgt tatggcgacc
     4741 atgctgctgc tgggtatcgt tacctgggaa gacgtggtta aaaataaagg cggctggaat
     4801 accttaatct ggtacggcgg tattatcggc ttaagctcct tattatcgaa agttaaattt
     4861 ttcgaatggt tagctgaagt ctttaaaaat aacctggcat ttgatggtca cggtaacgtt
     4921 gctttcttcg ttattatttt cctcagcatc atcgtgcgtt atttcttcgc ttccggtagt
     4981 gcctatatcg ttgctatgtt acctgtattt gccatgctgg cgaacgtctc cggcgcaccg
     5041 ttaatgttaa ccgcgctggc actgttgttc tccaactcct atggcggcat ggttactcac
     5101 tatggcggcg cggcaggtcc ggtcatcttt ggcgtgggtt acaacgatat taaatcctgg
     5161 tggttggtcg gtgcggtact gacgatatta accttcttgg tgcatatcac cctcggcgtg
     5221 tggtggtgga atatgctgat cggctggaac atgctgtaaa tatactcgtc atacttcaag
     5281 ttgcatgtgc tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg
     5341 attcactcac ttgtcgcctt cctgcaactc gaattattta gagtatatcc atttattatc
     5401 tttctgcgca cttcacggtg cgcagatatc tggagcattt gatgatcaag ttatctgaaa
     5461 aaggcgtgtt tctcgccagt aataacgaaa taattgccga agaacatttc accggcgaaa
     5521 ttaaaaaaga agaagcaaaa aaaggcacta ttgcctggtc tattctctct tctcataata
     5581 cgtccggaaa tatggataaa cttaaaatta agtttgattc attagcctct cacgatatta
     5641 cctttgttgg tattgtacag accgctaaag cgtccggaat ggaacgtttc ccgctgccgt
     5701 atgtgctgac caactgccat aactcactct gcgccgtcgg cggcaccatt aacggtgatg
     5761 accatgtttt tggtttatcg gcagctcagc gttatggcgg tatttttgtg cctccgcata
     5821 ttgcggtcat ccatcaatat atgcgtgaga tgatggctag cgggggcaaa atgatcctcg
     5881 ggtcagacag ccacacccgt tacggtgcat tagggacaat ggcagtcggt gagggtggcg
     5941 gtgagttggt aaaacagctg cttaatgaca cctgggatat cgactatccg ggcgtggttg
     6001 cggtgcatct gaccggaaaa cctgcgccgt atgtggggcc acaggatgtg gcgctggcta
     6061 tcattggcgc ggtgttcaaa aacggttacg tcaaaaacaa agtcatggag ttcgttggac
     6121 cgggcgttag cgcgctctct accgatttcc gtaacagcgt tgacgtgatg accacggaaa
     6181 ccacctgttt aagctccgtc tggcaaactg atgaagaagt gcataactgg ctggcgctgc
     6241 acggtcgcgg ccaggattac tgccagctta accctcaacc gatggcgtac tacgatggct
     6301 gcatcagcgt tgatttaagc gccatcaaac caatgattgc gctgccgttc cacccgagca
     6361 acgtgtatga aatcgacaca ctgaaccaga acctgaccga cattctgcgt gagattgaaa
     6421 ttgagtccga acgcgtggcg cacggtaaag ccaaactctc gctgctggat aaagtggaaa
     6481 atggtcgcct gaaagtgcag caggggatta tcgcgggctg ttctggcggt aactacgaaa
     6541 acgtcatcgc ggcggcgaat gcactgcgcg gtcaatcctg tggcaatgac accttctcgc
     6601 tggcggttta cccgtcatca cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag
     6661 cagatttgat tggcgcaggc gcaatcatca gaaccgcatt ctgtggccca tgctttggcg
     6721 cgggcgatac gccaatcaac aacggtttga gtattcgcca caccacgcgt aacttcccga
     6781 accgcgaagg ctctaagcca gccaatgggc agatgtcagc ggtggcgttg atggacgctc
     6841 gttctatcgc tgcgactgcg gcaaacggtg gctatttaac ctctgccagc gaacttgatt
     6901 gctgggacaa cgtgccggag tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc
     6961 agggctttgt gaaaggggca actcagcaac cgctgattta cggaccgaac attaaagact
     7021 ggccggaatt gggtgcgctg actgacaata tcgtcctgaa agtgtgctcg aagatcctcg
     7081 acgaagtcac caccaccgac gaactgattc cttccggtga aacctcttct tatcgttcaa
     7141 atccgattgg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     7201 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     7261 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     7321 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     7381 actgggggat gttaccgctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     7441 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     7501 tgatccatga agatgcgccg gtaacggaaa ttacgcttta catggaaagc ctgacggctg
     7561 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     7621 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgctggcg
     7681 catcttatcc gacctacaaa tcgcatcgaa ccgtaggccg gataaggcgt ttacgccgca
     7741 tccggcaaat agttaatagc tcttacttct tcgcctctgc aaccactttg ctgcccacgc
     7801 cgcggttatt gtattcccac atgcggttgt agttagtgtc attcagattg cgctgtattt
     7861 cgtcgttatc atctacgctg ccggtattac ccgcaaacgg acgattagag atcaccgcat
     7921 cggcccacgg tttagccgtg ttaaaacctt cgttgatggc gctatcacgg atcaccacct
     7981 gaccgttggt attagcatca acatccagcg agcggcccag ttgcgccacg ccatcaccgg
     8041 aagcattgaa acggctgttt acggcgagga aaccgtaata gatgttagac agcgtagccg
     8101 gtgcaaacac atatgcttct tgctgggtac gtgagttcac cacgcggaat tcggtgttat
     8161 cgaacaccac tgcgccgcga ccagaaacga tatccacatc cccttcaata tagctgttgg
     8221 tcaccagcgt acgcggctga cggttggttt ccagacggtt ctgcacaccg ctgttggtga
     8281 caaagaaggt gttctgacga ccgagaatgt taacgttgtt aatctgtact tggtcaccgt
     8341 cagtacgcag tgctaccgcc ggatggttac ctgcatctac gctatcgccc agcgtgtttt
     8401 cgatggtcag attttgcagt tgcagtccat tgttttgtga ccagaagacc gcagaacaca
     8461 gaacaccgat actgtcgctg cgtttgctct ggcagctatc gtacatatac cacgctggtt
     8521 tacctggcat atatttgccg cgcgggttga cgtcgtgacg ccagtcggca gggctcatgc
     8581 ccccatcaag ggaaagccca atcttcacat caatcggttt ttcacctgta ccgtacagag
     8641 tgattccacc tggagcagca gggacataca ccgttccctg atactcccca ggcatcacgg
     8701 caatatactg gcgcttgttg gtgcgcttga taattgccgc atctaccgcc gcctgaatcg
     8761 tggtatgcgt tacaccttga gtacccgccg ggccgacaac aaagtcaggt tgcgcaggca
     8821 gggttatcgg ggacggattc cacgctgcag cacctggtgt cagggatgca aaatagtgtt
     8881 gcgcatcgaa attcagcgct tcttttgccg acagaatcgg gcgagaagag gtaccaggcg
     8941 cagtttgatc agaaggacgt tgatcgggtg gtgttgagct acaggcggtc agcgtcacgc
     9001 caaaagccaa tgccagcgcc agacgggaaa ctgaaaatgt gttcacaggt tgctccgggc
     9061 tatgaaatag aaaaatgaat ccgttgaagc ctgctttttt atactaagtt ggcattataa
     9121 aaaagcattg cttatcaatt tgttgcaacg aacaggtcat tatcagtcaa aataaaatca
     9181 ttatttgatt tcaattttgt cccactccct gcctctgtca tcacgatact gtgatgccat
     9241 ggtgtccgac ttgtgcccga gaagatgttg agcaaactta tcgcttatct gcttctcata
     9301 gagtcttgca gacaaactgc gcaactcgtg aaaggtaggt ggatcccctt cgaaggaaag
     9361 acctgatgct tttcgtgcgc gcataaaata ccttgatact gtgccggatg aaagcggttc
     9421 acgacgagta gatgcaatta tggtttctcc gccaagaatc tctttgcatt tatcaagtgt
     9481 ttccttcatt gatatcccga gagcatcaac atgcaatgtt gtagggatgg caatttttac
     9541 gcctgttttg ctttgctcga cataaagata tccatctacg atatcagacc acttcatttc
     9601 gcataaatca ccaactcgtt gcccggtaac aacagccagt tccattgcaa gtctgagcca
     9661 acatggtgat gattctgctg cttgataaat tttcaggtat tcgtcagccg taagtcttga
     9721 tctccttacc tctgattttg ctgcgcgagt ggcagcgacc gggtttgttg ttatatggcc
     9781 ttcagctatt gcctctcgga atgcatcgct cagtgttgat ctgattaact tggctgatgc
     9841 cgccttgccc tcgtctatgt atccattgag cattgccgca atttcttttg tggtgatgtc
     9901 ttcaagtgga gcatcaggca gccccctcct tattgcttta attttgctca tgtaatttat
     9961 gagtgtcttc tgcttgattc ctctgctggc gaggattttt tcgtagcgat caagccatga
    10021 atgtaacgta acagaattat cactgttgat tctcgctgtc agaggcttgt gtttgtgtcc
    10081 tgaaaataac tcaatgttgg cctgtatagc ttcagtga
//
LOCUS       DAEBXD010000122        10009 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEBXD010000122 DAEBXD010000000
VERSION     DAEBXD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.122"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(262..606)
                     /locus_tag="KJL94_003429"
     CDS             complement(262..606)
                     /locus_tag="KJL94_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD4411653.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            738..2648
                     /locus_tag="KJL94_003430"
     CDS             738..2648
                     /locus_tag="KJL94_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBD4411654.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2706..3401
                     /gene="tsaB"
                     /locus_tag="KJL94_003431"
     CDS             2706..3401
                     /gene="tsaB"
                     /locus_tag="KJL94_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBD4411655.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3441..4022
                     /gene="yeaY"
                     /locus_tag="KJL94_003432"
     CDS             3441..4022
                     /gene="yeaY"
                     /locus_tag="KJL94_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBD4411656.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4227..5912
                     /gene="fadD"
                     /locus_tag="KJL94_003433"
     CDS             4227..5912
                     /gene="fadD"
                     /locus_tag="KJL94_003433"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBD4411657.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5994..7109
                     /gene="rnd"
                     /locus_tag="KJL94_003434"
     CDS             5994..7109
                     /gene="rnd"
                     /locus_tag="KJL94_003434"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBD4411658.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7163..8128)
                     /gene="yeaX"
                     /locus_tag="KJL94_003435"
     CDS             complement(7163..8128)
                     /gene="yeaX"
                     /locus_tag="KJL94_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBD4411659.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8184..8801)
                     /gene="yeaW"
                     /locus_tag="KJL94_003436"
                     /pseudo
     CDS             complement(8184..8801)
                     /gene="yeaW"
                     /locus_tag="KJL94_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9143..9709)
                     /locus_tag="KJL94_003437"
     CDS             complement(9143..9709)
                     /locus_tag="KJL94_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4411660.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9921..>10009
                     /locus_tag="KJL94_003438"
     CDS             9921..>10009
                     /locus_tag="KJL94_003438"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4411661.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2455 a         2437 c         2620 g         2497 t
ORIGIN      
        1 gaaaattaac ggtcagcccg cacgctataa ccagattgtt cagctaagaa agaaaaccag
       61 agttgctaat caaagggcga acacaacggc atggttatgt ttatttatat agttagtttt
      121 atagataaaa ttgaaattat aaatataaat aatgaattag cagaagcatc aacagtaata
      181 tcttattctc tggtcgattt atgtaaaacc gaccagtatt atcgataaat tatcgtctga
      241 cttattttgc ttcgataaag cttacaccgc agccacaatc ttaatttcaa ctttatactt
      301 cgggttcatc aaacccgctt gtaccgtgca gcgcaccggc gcatgacccg cgacaaccca
      361 ggcatcccac gctttattca tcgccgcgaa gtcgttttta tcggccagga aaatggtggc
      421 atccagaatg ctcgatttat tgctgccctg tttttccagc acggcgtcaa tctgtgccag
      481 cgtgttggcg gtttgctcaa aggcatcggc gtcgaggttt tccggtacac cagtgtagta
      541 gagcgtgttg ttgtggatta ctacatcaga ccagcgggct tcagcatcga tacgaacgat
      601 agtcatcatt ttgtcctcat tatacttcca ttttcgtgct gcaaagactg ccacaatgtg
      661 aggttgccgt caccacttca actggcgaaa gcgccctcat cctgacataa tccctgttca
      721 aatcaacagg gggtagtgtg acggacgatt ttgcaccaga cggtcagctg gcgaaagcga
      781 taccaggctt taagccgcga gaaccacagc gacagatggc ggtagccgtc acccaggcga
      841 tagaaaaagg ccagccgctg gtggtggaag caggaaccgg tacgggcaaa acctacgctt
      901 acctggctcc tgcgctgcgg gcgaaaaaga aagtcattat ctcgaccggc tcaaaagcgt
      961 tgcaggatca gctctacagc cgcgatttgc caacagtctc aaaggcattg aaatatacgg
     1021 gcaacgtggc gctgctgaaa gggcgctcaa actacctctg cctcgaacgt ctcgaacagc
     1081 aggcgctggc ggggggcgat ctgccggtac aaatcttaag cgatgtgatc ctgctgcggt
     1141 cctggtctaa tcaaacagtc gatggtgata tcagcacctg cgtcagcgtg gcggaagatt
     1201 cacaggcgtg gccgctggtc accagcacca acgacaactg tcttggcagc gactgcccga
     1261 tgtataaaga ttgctttgtg gtcaaagcac gtaaaaaagc gatggacgcc gatgtggtgg
     1321 tggtaaacca tcatctcttt ctggcggata tggtggttaa agagagtgga tttggcgagt
     1381 tgatccctga agcggacgtc atgatcttcg acgaagccca ccagctaccg gacattgcca
     1441 gccagtattt tggtcagtca ctctccagtc gacaactgct cgacctggca aaagacatca
     1501 ccatcgccta ccgcaccgaa ttaaaagaca cccagcagtt acaaaagtgc gctgatcgtc
     1561 ttgcccagag tgcgcaggat tttcgtctgc aactcggtga gccaggttat cgcggtaacc
     1621 tgcgtgagct gttagctaat ccgcaaattc agcgggcatt tttactgctc gatgacacct
     1681 tggaactttg ttatgatgtg gcgaaactgt cactggggcg ttccgccttg ctggatgcgg
     1741 catttgagcg cgccacgttg tatcgcacac ggctgaagcg gctaaaagag atcaatcagc
     1801 cgggctacag ctactggtac gaatgcactt cgcgccattt tactctggct ctcacgccgc
     1861 tcagcgtggc ggataaattc aaagagttaa tggcgcaaaa gcccggtagc tggatcttca
     1921 cctcagcaac gctgtcggtg aacgacgatc tgcatcattt cacctcgcgg cttgggatag
     1981 aacaggcgga gtcgttgcta ttgccaagcc cgtttgatta cagccgccag gcgttactct
     2041 gtgtgccgcg caatctgccg caaaccaacc agccaggttc tgctcgccag ttagcggcaa
     2101 tgctgcgacc gatcatcgaa gctaacaacg gtcgttgttt tatgctttgt acctcgcacg
     2161 ccatgatgcg cgatctggcc gagcagttcc gcgctaccat gacgcttccc gttttgttgc
     2221 agggggaaac cagtaaaggc caactgttgc agcagtttgt cagtgccggt aatgcgcttc
     2281 ttgtggcaac cagtagtttc tgggaagggg tggacgtgcg tggcgataca ttgtcattgg
     2341 taattatcga caaattgccg tttacctcgc cggatgatcc actgttaaaa gcgcgcatgg
     2401 aagattgtcg tttgcgcggt ggcgacccgt tcgatgaagt gcaactacca gatgccgtca
     2461 ttactctcaa acagggggta ggtcgactga ttcgcgacgc cgacgatcgt ggcgtgctgg
     2521 tgatttgtga ctatcggctg gtgatgcgtc cttacggcgc gacgtttctc gccagtctgc
     2581 cgcccgcgcc acgcacccgt gacattgccc gtgcggttcg tttccttgcg ataccatcct
     2641 ccaggtaatt tgttacggag tatggtatga tgcgcgcctt tttattgatc taagcacgaa
     2701 gacccatgcg aattctggct atcgataccg cgacagaggc ctgctctgtc gccctgtgga
     2761 acgacggtac tgtcaacgct cattttgagc tttgccctcg tgaacatact caacgaatct
     2821 taccgatggt gcaggatatc ctgaccacca gcggaacttc cctgactgat attaacgctc
     2881 tggcttacgg gcgcggcccc ggtagcttta ccggcgtgcg cattggtatt ggcatcgcgc
     2941 aagggctggc gcttggcgcg gaattaccga tgattggcgt ctccacgcta atgacgatgg
     3001 cgcaaggggc gtggcgcaaa aacggcgcaa cacgcgtgct gtcagccatt gacgcgcgaa
     3061 tgggcgaagt ttactgggcc gaatatcagc gtgatgaaaa cggtatctgg cacggtgaag
     3121 aaaccgaagc cgtactcaaa cccgaactcg ttcatgaacg aatgcaacag ctttccggcg
     3181 aatgggtgac ggtagggacg ggctggcaag cctggccgga tctcggtaaa gagagcgggc
     3241 tggttttgcg cgatggcgaa gtgttactcc ccgctgcaga agatatgctg ccgattgctt
     3301 gtcagatgtt tgccgagggt aaaacggtgg cggtggaaca tgccgagccg gtttatttgc
     3361 gtaacaacgt cgcatggaag aaacttccgg gcaaagaatg aatcttaaga gtatgccctg
     3421 agaaaaaaag gagtcgcatc atggcggttc aaaagaatgt tatcaaaggc atactggcag
     3481 gtacgtttgc gctaatgctg agcggttgtg tcactgtgcc ggacgccatt aaaggcagca
     3541 gtcccacgcc gcaacaagat ttagttcggg tgatgagtgc gccgcagctg tacgttggtc
     3601 aggaggcacg ctttggtggc aaagtggttg cggtacaaaa ccagcaaggg aaaacccgcc
     3661 tggaaattgc taccgtaccg ctggacagcg gagctaggcc gacgctggga gaaccttctc
     3721 gcggtcgcat ttatgccgat gtgaacggtt ttctggaccc ggtggatttc cgtggacaac
     3781 tggttacggt agtcgggcca atcaccggtg cggttgacgg caaaatcggc aatacgccct
     3841 ataaatttat ggtgatgcaa gtaacgggtt acaaacgttg gcatttaacc cagcaggtga
     3901 ttatgccgcc tcagccgatt gatccatggt tttatggcgg tcgtggctgg ccctatggct
     3961 acggcggatg gggctggtat aatcccggcc ccgcgagagt acaaacagtt gtaaccgaat
     4021 aattgcttgt ttttaaagaa aaagaaacag cggctggtcc gctgtttctg cattcttacg
     4081 gtaaagataa aaataaatag tgacgcgctt cgcaaccttt tcgttgggta attatcaagc
     4141 tggtatgatg agttaatatt atgttaacag tgtgtatatc atttggggtt gcgatgacga
     4201 cgaacacgca ttttagaggt gaagaattga agaaggtttg gcttaaccgt tatcccgcgg
     4261 acgttccgac ggagatcaac cctgaccgtt atcaatctct ggtagatatg tttgagcagt
     4321 cggtcgcgcg ctacgccgat cagcctgcgt ttgtgaatat gggggaggtg atgaccttcc
     4381 gcaagctgga agaacgcagt cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga
     4441 agaaaggcga tcgcgttgcg ttgatgatgc ctaacttatt gcaatatccg gtggcgctat
     4501 ttggcatttt gcgtgccggg ataatcgtcg taaacgttaa cccgttgtat accccgcgtg
     4561 agcttgagca tcagcttaac gatagcggcg catcggcgat tgttatcgtg tctaactttg
     4621 cccacgctct ggaaaaagtg gttgataaaa ccgccgttca gcacgtaatt ctgacccgta
     4681 tgggcgatca gctatctacg gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca
     4741 agcgcttggt gccgaaatac catctgccag atgccatttc atttcgtagc gcactgcata
     4801 acggctaccg gatgcagtac gtcaaacccg aactggtgcc ggaagattta gccttcctgc
     4861 aatacaccgg cggcaccaca ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc
     4921 tggcgaacct ggaacaggtc aacgccacct atgggccgtt gctgcatcca ggtaaagaac
     4981 tggtagtaac ggcactgccg ctgtatcaca tttttgccct gaccattaac tgcctgctgt
     5041 ttatcgaact gggtgggcag aacctgctta tcactaaccc gcgtgatatt ccagggttgg
     5101 taaaagagtt agcgaaatat ccgtttaccg ctatcactgg cgttaacacc ttgttcaatg
     5161 cgttgctgaa caataaagag ttccagcagc tggatttctc cagtctgcat ctttccgcag
     5221 gcggtgggat gcctgttcag caagtggtgg cagaacgctg ggtgaaactg accggacagt
     5281 atctgctgga aggttatggc ctgaccgagt gcgcgccgct ggtgagcgtt aacccgtatg
     5341 atattgatta tcatagcggt agcatcggtt tgccggtgcc atcgacggaa gccaaactgg
     5401 tggatgacga cgacaacgaa gtaccaccgg gtcaaccggg tgagctttgt gtcaaaggac
     5461 cgcaggtgat gctgggttac tggcagcgtc cggatgctac agatgagatc atcaaaaatg
     5521 gctggttaca caccggcgac atcgcggtga tggatgaaga aggattcctg cgcattgtcg
     5581 atcgtaaaaa agacatgatt ttggtttccg gttttaacgt ctatcccaac gagattgaag
     5641 atgtcgtcat gcagcatcct ggcgtacagg aagtcgcggc tgttggcgta ccttccggct
     5701 ccagtggtga agcggtgaaa atcttcgtag tgaaaaaaga tccatcgctt accgaagagt
     5761 cactggtgac tttttgccgc cgtcagctca cgggatacaa agtaccgaag ctggtggagt
     5821 ttcgtgatga gttaccgaaa tctaacgtcg gaaaaatttt gcgacgagaa ttacgtgacg
     5881 aagcgcgcgg caaagtggac aataaagcct gagcgttaag tcagtcgtca gacgccggtt
     5941 aatccggcgt tttttttgac gcccactaaa gagaaaacaa tttgaattac caaatgatta
     6001 ccacggacga tgcgctggct tctttgtgtg aagccgtccg tgtctttccg gcgatagccc
     6061 tggatactga atttgttcgt acgcgcactt attacccgca gctggggttg attcaacttt
     6121 tcgatggcga gcatctggcg ctaattgatc ctctcgggat caccgactgg tcaccgctga
     6181 aagcgatcct gcgcgatccg tccatcacaa aatttctcca tgcaggcagt gaagatctgg
     6241 aagtgttcct caatgtcttt ggcgaattac cacaaccctt gattgacacg caaatccttg
     6301 ctgccttctg cggacgcccg atgtcatggg gtttcgcttc catggtggaa gagtattccg
     6361 gcgttacgct ggacaagagt gaatcgcgca ccgactggct ggccagaccg ctgaccgaac
     6421 gtcagtgtga atacgcagcg gcggatgtct ggtatctgtt accgatcacc gccaagctta
     6481 tggtagaaac ggaggcctcc ggctggctac ctgcggcgct ggatgaatgc cgcctgatgc
     6541 aaatgcgtcg tcaggaagtc gttgcgccgg aagatgcctg gcgtgatatc accaatgcct
     6601 ggcagttacg cacacgccaa ctggcctgtc tgcaactgtt agccgactgg cgactgcgca
     6661 aggcgcgaga gcgtgatctg gcggtgaact ttgtcgtgcg tgaagagcat ttgtggtcgg
     6721 tcgcgcgtta tatgccggga agtttaggcg aactggacag cctgggttta tccggtagcg
     6781 aaatccgctt tcacggtaaa acgctgctgg cgctggtgga aaaagcgcag acattgccgg
     6841 aagatgcctt accgcagccg atgcttaacc tgatggacat gcctggttat cgtaaagcgt
     6901 ttaaagcgat taagtcgctg attactgacg tgagcgaaac gcataagatc agcgccgaat
     6961 tgctggcatc gcgtcggcaa atcaaccaac tgctgaactg gcactggaaa ctgaaaccgc
     7021 agaacaattt gccggagctg atttccggct ggcgtggtga gctgatggcg gaagcattac
     7081 acaatttatt gcaggaatat ccgcagtaaa atcttccgaa gccggactgg gcgcgctcag
     7141 cgccacatcc ggctttggca aactacaaat ccaacaccag gcgtttaccc ttcgcacgcg
     7201 aacaacagat caacatactt tgctggctgg cacgctcttc atcgctaaaa tactgatccc
     7261 gatggtcagc ttcaccttcc agtattgccg tttcgcaggt tccgcatacc ccttcacgac
     7321 ataaacattc cactttcgcg gctttattat tttcaataac ctgcaaaata gtcatctctt
     7381 ccggcaccac aaactctttt ccggaacggg caagcaccag ggtaaatgca tcgccggttt
     7441 tgtcttcgat agcaaattgc tcaaagtgca gtgtatcggc ggcgatgtcc agacgcgcag
     7501 cttcacttct taccgcttca attagcgcct cggggccaca ggtataaacg tgtgtgccag
     7561 gttcgatatc cgccaataat cgcgctaatt ccagtcgtgt tccggttgat gaatgatgca
     7621 aatggacttt ctcagcctgc ggatgctgga ctagctcagc acgaaatgcg caactttctg
     7681 gatttcgcga gcagtaatgt agctgccagt cgatgtcgct gtgttgcaac tctgccatgt
     7741 gcgacaggaa aggggggata ccaataccgc ccgcgataaa cagatgcttt ctggctgagg
     7801 gaattagcgc aaacaggtta ttaggcgttg agatcgttaa ccgatcgccc acttttacct
     7861 gctgatgcaa aaagcgggaa ccgccgcgcg agttttcttc cagtcgaacg gcaatctgat
     7921 aactagaggt gttatgcggt gaactcagta gtgaatacgc attgctgtac tggttatcac
     7981 catcgctcat ctgcactagg atgtgacttc ctccggtgaa cgcaggcagc ggtttgccat
     8041 cggttgccac cagcgtgaag cgtttcacct gttcggtaag gggttcaacc tggctcacct
     8101 gtacttcaaa catttgatag tctgacattg ctgtactcca tcaaaaatca gcccagcggc
     8161 ggaaaatacc gccgccgatg tcattagtcc ttaaacacct gcgccagcag attatggaaa
     8221 tgggcgatac catgttcgga aatgccacta ccgctactgt cggccatgat gcgcccctga
     8281 ccacgatagc cacgcgattt cagccctttc tgtacgcttt caaccagacg taaatcttcc
     8341 ggatggaaca catcgcgata ccactcaatc agcgattttt gctcgtcggt taactcttca
     8401 ttggtgaagt aaatatcgta gttttgcagg gtagtttcag aatccaccgg gaattcataa
     8461 atgaccgtca tcatcccttt gatcggggtg acgttcagca tcgtgcacgg ccacagccag
     8521 aaaccgtgga atgccgcatc cgtaccctct tcaaatttaa acgactgttc ggacggtttg
     8581 gcgaaaccgt attgcagcgt ccagttaccg tgcatggtgt gccagtaacg atcaacctgt
     8641 acggagtcgg agaaacctag atgcgccgga ccacagtgat agcactcgag atagttatcg
     8701 acaatgttct tccagttggc aggcgtgcgg gtggtaaagc gggccgctag tttcagattg
     8761 tggacttccg ggcaggcttc cagcactttc gcgcccagac cggtgtattg attcacttga
     8821 agtacgaaaa aaaaccggga ggacattgga ttattcgggt tctgatggga ttagatttgg
     8881 tggggcttgc aagcctgtag cgcaaatttt agtcgttaat caatgaaacg cgaaagatag
     8941 taaaaaattg cttttgtttc attgaaaata cgaaaaacaa aaacactgca aatcatttca
     9001 ataacagctt caaaaaacgt tcaaaaccga taacaaccag gctgtaacca aatgactcat
     9061 atcacaaatc agctcatgcc gtttaggtat gttacatgtg tgattatgtg aggtgaagta
     9121 tgttttagct ggttcatggt tgttatacgg ctttttttac atcttgtggt tcttgtgaag
     9181 gagctacaag accttcctgt tcataaatac catactttga caaaatcttt ttacgagtat
     9241 ctgttggtaa tgattcaagc attttggctg tttgtaaggt ttgctgcgaa ataccttgca
     9301 aatcagcctg gatggaatca tcggaagaaa aaatctcagg cataagagct tcaaatcctt
     9361 tctttctgaa caactcaatc gcagaatgca gttccttgtc ctctaacaat tcgacaattg
     9421 cgatcagttt agttttcaat gtgctgcggt caggttgaga atttttattc tcttgattat
     9481 ttttatgctc aaccattggc atgctctgac tttggataaa ccactctatt ggacgccctg
     9541 ttgcgtaggc tatttgtgct aatcgatcta aagacgggta tgtcttgcca gacaggtagt
     9601 ttctgataac cccatcagaa agtccgcact tagctgcaaa cgcccggctt gattctccat
     9661 tcattgcctc ttcaaggcgt tccttgaaac gcattatttc cgggggcata aaaatgcgct
     9721 ctgcattttt gctttctttt tcacctgtca tgcccaatct ctctgttata taaagaattt
     9781 tacagaattc tttgatttgc actggttaaa ataaaaatga gcatagctct tgaattgagc
     9841 atttttctgc ggtatcgttt ttcccgatag cggtagccgg tatcagttac cgaaaacgtt
     9901 aagagagtaa ggatcgcaga atgtcagcaa aaaaccaaga ctggcacgca gagcagataa
     9961 aggctgctgt tcgtatgaag gggaagacac tatctcaatt atccagaga
//
LOCUS       DAEBXD010000123         9951 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEBXD010000123 DAEBXD010000000
VERSION     DAEBXD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.123"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="KJL94_003439"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="KJL94_003439"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBD4411662.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="KJL94_003440"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="KJL94_003440"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBD4411663.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="KJL94_003441"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="KJL94_003441"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBD4411664.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="KJL94_003442"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="KJL94_003442"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBD4411665.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="KJL94_003443"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="KJL94_003443"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBD4411666.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="KJL94_003444"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="KJL94_003444"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBD4411667.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="KJL94_003445"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="KJL94_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="KJL94_003446"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="KJL94_003446"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBD4411668.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="KJL94_003447"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="KJL94_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBD4411669.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9951)
                     /locus_tag="KJL94_003448"
     CDS             complement(8974..>9951)
                     /locus_tag="KJL94_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4411670.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2506 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DAEBXD010000124         9927 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEBXD010000124 DAEBXD010000000
VERSION     DAEBXD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.124"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..338
                     /locus_tag="KJL94_003449"
     CDS             <1..338
                     /locus_tag="KJL94_003449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411671.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            526..1641
                     /locus_tag="KJL94_003450"
     CDS             526..1641
                     /locus_tag="KJL94_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411672.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2320..2538
                     /locus_tag="KJL94_003451"
     CDS             2320..2538
                     /locus_tag="KJL94_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBD4411673.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="KJL94_003452"
     CDS             2756..2998
                     /locus_tag="KJL94_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411674.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KJL94_003453"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KJL94_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBD4411675.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KJL94_003454"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KJL94_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD4411676.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KJL94_003455"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KJL94_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBD4411677.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KJL94_003456"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KJL94_003456"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD4411678.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="KJL94_003457"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="KJL94_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBD4411679.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2396 c         2543 g         2498 t
ORIGIN      
        1 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
       61 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
      121 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
      181 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
      241 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
      301 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
      361 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      421 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
      481 gaaaaagttg aggatatgtg tgaaagaatg aaaggtggca tctctatgtt taaccacacc
      541 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
      601 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
      661 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
      721 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
      781 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
      841 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacaagccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattg caggacttat tcatttc
//
LOCUS       DAEBXD010000125         9644 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEBXD010000125 DAEBXD010000000
VERSION     DAEBXD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.125"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KJL94_003458"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KJL94_003458"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBD4411680.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="KJL94_003459"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="KJL94_003459"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBD4411681.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="KJL94_003460"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="KJL94_003460"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD4411682.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="KJL94_003461"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="KJL94_003461"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD4411683.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="KJL94_003462"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="KJL94_003462"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD4411684.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="KJL94_003463"
     CDS             5060..6193
                     /locus_tag="KJL94_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD4411685.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="KJL94_003464"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="KJL94_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD4411686.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KJL94_003465"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KJL94_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBD4411687.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KJL94_003466"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KJL94_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBD4411688.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KJL94_003467"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KJL94_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBD4411689.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KJL94_003468"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KJL94_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBD4411690.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="KJL94_003469"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="KJL94_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBD4411691.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2261 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tctt
//
LOCUS       DAEBXD010000126         9596 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEBXD010000126 DAEBXD010000000
VERSION     DAEBXD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.126"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="KJL94_003470"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="KJL94_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBD4411692.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="KJL94_003471"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="KJL94_003471"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBD4411693.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="KJL94_003472"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="KJL94_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBD4411694.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="KJL94_003473"
     CDS             complement(2404..2607)
                     /locus_tag="KJL94_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411695.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="KJL94_003474"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="KJL94_003474"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD4411696.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="KJL94_003475"
     CDS             complement(3803..4015)
                     /locus_tag="KJL94_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBD4411697.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="KJL94_003476"
     CDS             4275..4583
                     /locus_tag="KJL94_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBD4411698.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="KJL94_003477"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="KJL94_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBD4411699.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="KJL94_003478"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="KJL94_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBD4411700.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="KJL94_003479"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="KJL94_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBD4411701.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="KJL94_003480"
     CDS             complement(8479..8982)
                     /locus_tag="KJL94_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4411702.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9596)
                     /locus_tag="KJL94_003481"
     CDS             complement(9070..>9596)
                     /locus_tag="KJL94_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4411703.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2448 c         2283 g         2483 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attaccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttcct
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat tatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accactgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatat gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagc
//
LOCUS       DAEBXD010000127         9499 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEBXD010000127 DAEBXD010000000
VERSION     DAEBXD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.127"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..190)
                     /locus_tag="KJL94_003482"
     CDS             complement(<1..190)
                     /locus_tag="KJL94_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBD4411704.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KJL94_003483"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KJL94_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBD4411705.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KJL94_003484"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KJL94_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBD4411706.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KJL94_003485"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KJL94_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBD4411707.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KJL94_003486"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KJL94_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KJL94_003487"
     CDS             complement(3262..3381)
                     /locus_tag="KJL94_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBD4411708.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KJL94_003488"
     CDS             complement(3342..3527)
                     /locus_tag="KJL94_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411709.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KJL94_003489"
     CDS             3628..3801
                     /locus_tag="KJL94_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411710.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KJL94_003490"
     CDS             3803..4147
                     /locus_tag="KJL94_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBD4411711.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KJL94_003491"
     CDS             4151..4486
                     /locus_tag="KJL94_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBD4411712.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KJL94_003492"
     CDS             complement(4543..6864)
                     /locus_tag="KJL94_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4411713.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KJL94_003493"
     CDS             complement(6981..7190)
                     /locus_tag="KJL94_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411714.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KJL94_003494"
     CDS             complement(7941..9464)
                     /locus_tag="KJL94_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD4411715.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2663 a         2203 c         2081 g         2552 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tacatgaaa
//
LOCUS       DAEBXD010000128         9483 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEBXD010000128 DAEBXD010000000
VERSION     DAEBXD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.128"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KJL94_003495"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KJL94_003495"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBD4411716.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KJL94_003496"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KJL94_003496"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBD4411717.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KJL94_003497"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KJL94_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBD4411718.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="KJL94_003498"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="KJL94_003498"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBD4411719.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="KJL94_003499"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="KJL94_003499"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBD4411720.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="KJL94_003500"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="KJL94_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBD4411721.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="KJL94_003501"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="KJL94_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBD4411722.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="KJL94_003502"
     CDS             complement(8064..9206)
                     /locus_tag="KJL94_003502"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBD4411723.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..>9483)
                     /locus_tag="KJL94_003503"
     CDS             complement(9215..>9483)
                     /locus_tag="KJL94_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBD4411724.1"
                     /translation="NSEYYTRFINDDTVFIHYTGITKPWHDWANYASADYFRNIYNIS
                     PWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         2630 a         2189 c         2075 g         2589 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttt
//
LOCUS       DAEBXD010000129         9440 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEBXD010000129 DAEBXD010000000
VERSION     DAEBXD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.129"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            227..751
                     /locus_tag="KJL94_003504"
     CDS             227..751
                     /locus_tag="KJL94_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4411725.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            832..1506
                     /locus_tag="KJL94_003505"
     CDS             832..1506
                     /locus_tag="KJL94_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD4411726.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1522..4002
                     /locus_tag="KJL94_003506"
                     /pseudo
     CDS             1522..4002
                     /locus_tag="KJL94_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            4018..5051
                     /locus_tag="KJL94_003507"
                     /pseudo
     CDS             4018..5051
                     /locus_tag="KJL94_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5133..5471)
                     /gene="rcnB"
                     /locus_tag="KJL94_003508"
     CDS             complement(5133..5471)
                     /gene="rcnB"
                     /locus_tag="KJL94_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBD4411727.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5690..6538)
                     /gene="rcnA"
                     /locus_tag="KJL94_003509"
                     /pseudo
     CDS             complement(5690..6538)
                     /gene="rcnA"
                     /locus_tag="KJL94_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6660..6932
                     /gene="rcnR"
                     /locus_tag="KJL94_003510"
     CDS             6660..6932
                     /gene="rcnR"
                     /locus_tag="KJL94_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBD4411728.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7024..7120
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7155..7943
                     /gene="thiM"
                     /locus_tag="KJL94_003511"
     CDS             7155..7943
                     /gene="thiM"
                     /locus_tag="KJL94_003511"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBD4411729.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7940..8740
                     /gene="thiD"
                     /locus_tag="KJL94_003512"
     CDS             7940..8740
                     /gene="thiD"
                     /locus_tag="KJL94_003512"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBD4411730.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8805..>9440
                     /locus_tag="KJL94_003513"
     CDS             8805..>9440
                     /locus_tag="KJL94_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBD4411731.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEY"
BASE COUNT         2631 a         2011 c         2288 g         2510 t
ORIGIN      
        1 ttattgatat caacaatacc atttacataa atctacatta attaatagat gtgataaata
       61 tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt tttaaatcaa
      121 taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt ttccaagaga
      181 tacagactct taacaaccaa aaataaattt tattaaggaa catattatga aacgttcaat
      241 tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg ctgcagacgt
      301 tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta aattcgaagc
      361 gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct ttgaaggtaa
      421 agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt taaaaattag
      481 ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca aaataaccgg
      541 taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg atgtcctaga
      601 tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat atgctattcc
      661 gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc
      721 ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt ctccaggtaa
      781 taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa gatgaaagga
      841 ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat tgttatctac
      901 gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag
      961 ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc gttaccacca
     1021 gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg ggcaaattcc
     1081 gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa agaaagcatt
     1141 ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg caagaatgca
     1201 ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg
     1261 gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata
     1321 aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc
     1381 aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat caaaagtaat
     1441 aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat tagtgacaaa
     1501 atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg
     1561 ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata
     1621 ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg
     1681 gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt
     1741 gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg gttaggcatt
     1801 aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt
     1861 gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcttgg
     1921 gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg
     1981 ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc gggtaacaac
     2041 aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca actgcattct
     2101 gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat
     2161 ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat gtacacatca
     2221 agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg
     2281 ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaacgcg
     2341 ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc tcctggcccg
     2401 tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt tagcgtgaaa
     2461 gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc aaatatgctg
     2521 caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga aggggcgagc
     2581 aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt attgacgctg
     2641 tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac gggttggaac
     2701 acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc
     2761 gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg
     2821 acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac atttaacgat
     2881 cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat ctatgacatt
     2941 gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa tatgagccag
     3001 tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg
     3061 cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg gcggataagc
     3121 tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa acgttttaat
     3181 atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg tcggcaaata
     3241 tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa tacgggatta
     3301 tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag tcatcaacat
     3361 cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt tgcgacagtg
     3421 aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt ttcagggggc
     3481 attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg
     3541 atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg cacaacaaac
     3601 cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca cctgatgctg
     3661 gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat tgccgcccct
     3721 tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc ctggtttata
     3781 aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa tgatatccat
     3841 ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac caatgaaata
     3901 ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac aatcaccttc
     3961 ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg tggttctatg
     4021 gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag ctatgcggca
     4081 tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt tgcacttact
     4141 caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt gagtacaaat
     4201 actatcaccc ctatgagccc atcggatatt attgttggac tttataacga taccattaaa
     4261 ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa taatcagagc
     4321 agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat
     4381 atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt atccagtgct
     4441 tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt aggtggcaag
     4501 tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc aggtgtctat
     4561 tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca ggacgattta
     4621 ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa caaagcaaca
     4681 ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg tcaacaaaat
     4741 caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac caacagcaac
     4801 acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt aatggttcgc
     4861 cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta aaagaaattg
     4921 cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc agttactacg
     4981 tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta atcaacgtga
     5041 aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata acaaggtgtc
     5101 tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat ctcaccgtcg
     5161 taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag
     5221 gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg cttaatgttg
     5281 tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg cttaaagtca
     5341 gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga acccgcggtg
     5401 gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa catcttattt
     5461 ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata tctttcctgt
     5521 catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg taaaaagtgt
     5581 aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga aatagctgat
     5641 ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt tatcgcatta
     5701 tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac agactggaaa
     5761 aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg acctgctgaa
     5821 cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca atgctgaaac
     5881 tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc aacagcacgg
     5941 taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa atttgccagt
     6001 tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg
     6061 catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt
     6121 ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca tgcccgtgca
     6181 tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag aacgcggtgc
     6241 caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga gcagtaaagc
     6301 gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta tgcgaaatgg
     6361 ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg atgataaacg
     6421 ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca agtaagatgg
     6481 cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat tcggtcatga
     6541 taataattct tagtattaat tcggcaatct gattctactc ccccccagta cctgatacta
     6601 cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca ggtgttgcca
     6661 tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag attcagggcc
     6721 aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca gttttacaac
     6781 agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc
     6841 tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat ctggatgtcg
     6901 ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta aaactgccgt
     6961 ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga tttatcatcg
     7021 caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg tatcacctga
     7081 tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc ttcacgttat
     7141 ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg cacgcgttac
     7201 acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg gtgcaaacct
     7261 ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc gaaaccgaag
     7321 aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc acactgacgc
     7381 agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct caaacacgct
     7441 ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt tgtcatgaac
     7501 ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg gcattagctg
     7561 gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac gcgatacccg
     7621 ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc gaggtggatt
     7681 atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta atgaccaaag
     7741 tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg ttaccaggcg
     7801 atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga gaacgcgcag
     7861 tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg ctctggcaat
     7921 tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg gtactgatcc
     7981 gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg gcgcttatgg
     8041 ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt cggtgtatcg
     8101 cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg tgcgaatcga
     8161 taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg cagaacggtt
     8221 gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa aaagcggcga
     8281 cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc cacaggtttc
     8341 attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac acgcgcgcac
     8401 cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg gcgcagtgct
     8461 aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta cccgcgaggg
     8521 tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca ctggttgtac
     8581 actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg acaccgtaca
     8641 ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg aagttggtca
     8701 cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact atagctgctt
     8761 tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag ctaagaatta
     8821 ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc atcgttgcaa
     8881 tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt acagactaca
     8941 tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct gccagttaca
     9001 ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag cgggtggcaa
     9061 aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg gaaggcgaaa
     9121 agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat ggcctgctgc
     9181 gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg cgattatttc
     9241 tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta gacgaacggg
     9301 gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa atggtcgaaa
     9361 aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac accaatctgg
     9421 cgggctattt caatgaatat
//
LOCUS       DAEBXD010000130         9337 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEBXD010000130 DAEBXD010000000
VERSION     DAEBXD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.130"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KJL94_003514"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KJL94_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBD4411732.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KJL94_003515"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KJL94_003515"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBD4411733.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="KJL94_003516"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="KJL94_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KJL94_003517"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KJL94_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBD4411734.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KJL94_003518"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KJL94_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBD4411735.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="KJL94_003519"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="KJL94_003519"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBD4411736.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="KJL94_003520"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="KJL94_003520"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBD4411737.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="KJL94_003521"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="KJL94_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..>9337
                     /gene="rhaM"
                     /locus_tag="KJL94_003522"
     CDS             9042..>9337
                     /gene="rhaM"
                     /locus_tag="KJL94_003522"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBD4411738.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQ"
BASE COUNT         2264 a         2450 c         2452 g         2171 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaaga
//
LOCUS       DAEBXD010000131         9153 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEBXD010000131 DAEBXD010000000
VERSION     DAEBXD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.131"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..923
                     /locus_tag="KJL94_003523"
     CDS             <1..923
                     /locus_tag="KJL94_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4411739.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="KJL94_003524"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="KJL94_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBD4411740.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KJL94_003525"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KJL94_003525"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBD4411741.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="KJL94_003526"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="KJL94_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBD4411742.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="KJL94_003527"
     CDS             4446..4805
                     /locus_tag="KJL94_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBD4411743.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="KJL94_003528"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="KJL94_003528"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBD4411744.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="KJL94_003529"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="KJL94_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBD4411745.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="KJL94_003530"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="KJL94_003530"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD4411746.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2217 c         2469 g         2373 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAEBXD010000132         9014 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEBXD010000132 DAEBXD010000000
VERSION     DAEBXD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.132"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KJL94_003531"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KJL94_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBD4411747.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KJL94_003532"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KJL94_003532"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBD4411748.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KJL94_003533"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KJL94_003533"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBD4411749.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KJL94_003534"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KJL94_003534"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBD4411750.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="KJL94_003535"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="KJL94_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBD4411751.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="KJL94_003536"
     CDS             5545..6018
                     /locus_tag="KJL94_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBD4411752.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="KJL94_003537"
     CDS             6047..6589
                     /locus_tag="KJL94_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBD4411753.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="KJL94_003538"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="KJL94_003538"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBD4411754.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="KJL94_003539"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="KJL94_003539"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBD4411755.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="KJL94_003540"
     CDS             7986..8243
                     /locus_tag="KJL94_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBD4411756.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="KJL94_003541"
     CDS             complement(8219..8773)
                     /locus_tag="KJL94_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBD4411757.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2322 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DAEBXD010000133         8909 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEBXD010000133 DAEBXD010000000
VERSION     DAEBXD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.133"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(91..981)
                     /gene="malG"
                     /locus_tag="KJL94_003542"
     CDS             complement(91..981)
                     /gene="malG"
                     /locus_tag="KJL94_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBD4411758.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(996..2540)
                     /gene="malF"
                     /locus_tag="KJL94_003543"
     CDS             complement(996..2540)
                     /gene="malF"
                     /locus_tag="KJL94_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBD4411759.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2694..3884)
                     /gene="malE"
                     /locus_tag="KJL94_003544"
     CDS             complement(2694..3884)
                     /gene="malE"
                     /locus_tag="KJL94_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBD4411760.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4249..5364
                     /gene="malK"
                     /locus_tag="KJL94_003545"
     CDS             4249..5364
                     /gene="malK"
                     /locus_tag="KJL94_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBD4411761.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5436..6776
                     /gene="lamB"
                     /locus_tag="KJL94_003546"
     CDS             5436..6776
                     /gene="lamB"
                     /locus_tag="KJL94_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBD4411762.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            6928..7848
                     /gene="malM"
                     /locus_tag="KJL94_003547"
                     /pseudo
     CDS             6928..7848
                     /gene="malM"
                     /locus_tag="KJL94_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
     gene            8077..8909
                     /locus_tag="KJL94_003548"
                     /pseudo
     CDS             8077..8909
                     /locus_tag="KJL94_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         2250 a         2236 c         2249 g         2174 t
ORIGIN      
        1 aaggcgctcc gtggagcgcc gaataatagt cacaagttgg gataacgtaa gttgagggtg
       61 cagcggcata acattggcag aacaacatct ttaacctttc acaccacctg ccgtcaggcc
      121 gttgaccagc cagcgttgag ccagcaagaa gacgatggtg atcggtaatg cagacatcac
      181 ggcagcggcg gcaaagtcac cccacaggta gttttgcggg ttgaggtatt gctgcatccc
      241 cacggccagg gtgtagctgt ttacgtcacg cagtaacagc gacgcgaccg gaacttcagt
      301 aatggcagcg ataaacgaca ggatgaatac caccgccaga atcggtactg acagcggcaa
      361 caggacaagg cggaaggcct gccacggtgt cgcaccatcc agcgcagccg cttcttccag
      421 cgaactgtcg atggtttcga aatagccttt gatggtccag acatgcagcg caatcccacc
      481 cagatacgcg aaaattacgc cgccgtgagt attcaggcca atgaatggaa tgtactcacc
      541 cagacgatca aacaacgcat acaacgcgac cagtgaaagt actgccggga acatctggaa
      601 aatcagcatt cctttcagca gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta
      661 ggcgcaggtg gtggagagcg ccacaatgcc aatcgcggaa atcccagcga cctttaccga
      721 gttccacagc cacagcagta ccgggaatgg cggtggcgta atgcgaccat cagcctgttc
      781 aacgctaaaa cctaacgcca gtttccagtg atcccaggag atttgctccg ggatcaggct
      841 accggtcgcg aagtttccct gacgcagcga gatagcgacg accatcagca gcgggaacat
      901 aatcgccgcg ataaaaagta gcagtagcag gtgagtaata aacaaacgtg ctttttgcga
      961 tttcggttgg accattgcca tttttgttat ctcccttaat caaacttcat tcgcgtggct
     1021 ttcaggttca ctatcgccag cgcacccacc agcaggaaga tcagcgtggc aattgctgcc
     1081 gccagaccga agtcctgacc cccgccgcct tcaaaagcga tacggtaggt gtagttaaca
     1141 agcaggtcgg tataaccggc tggcgtggtc gtgccaagac gatccgggcc gccgttggtt
     1201 aacagctgaa tcagcacgaa gttgttaaag ttaaaggcga agctggcgat catcagcggc
     1261 gtcagcggtt taatcagcag cggcagcgta atcttaaaga agttctggaa cggacctgcg
     1321 ccatccattg ctgaggcttc atacaaatcg tccggaatcg ctttcagcaa gcccatgcag
     1381 aggatcatca tgtacggata acccagccag gtattgacga taatcagcat cgtgcgagcg
     1441 gtggtcggat cgctgaacca ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg
     1501 atttcaccga agctctggtt aaacaatcct ttgaaaatca agattgaaat gaacgatggc
     1561 accgcgtagg gcagaatcag caggacgcga tagaccgctt tgccgcgcaa cgcttcccac
     1621 tgcaccagac atgccagaac catgccgacc gccaccgtta aaaagacagt gatcagcgag
     1681 aacaccacgg tccagacgaa aatggcgagg aacggtttct gaatgccttc gtcggtaaag
     1741 acgcgggtaa agtttttcca gccggtggtc acggtgtaac cggggcttag cttttcatcc
     1801 ccccagttgc cgtcggcggt aatggactgg taaaagccaa tttggttatt cggacgatat
     1861 ttcacgccac tctgattatt cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc
     1921 gtgccagaaa actggcgcag ggagctcatc atcactttgt tgccatccgg cagaatggcg
     1981 gtaatgtcac tcagcgcctg acgattctgg gtaatcacgc gcagattcgc gcgttcgcct
     2041 tcgggctggg cggtcgtttc tttcagttgc agtttttgct cgccgccaaa tttaaacgcg
     2101 tcggagaggt aatttttgcc ggtttcgccg tcgctgagcg ccagttgcca ctcatcgccc
     2161 gccgggtaaa gaccaaagtt ataggttttg cctgcttgcc aggagcgatc taacaacact
     2221 tcctgcgcac gttcaaaagt cagctggtta gtgctgctgt agttggtgaa ggcaatggcg
     2281 atggtgcaga ccagagggaa gaggacgaat aatcccattc cagccattcc cgggtaaaca
     2341 tagcgccaga cgtaggcttt acgattggcg aaaatataca gccccgctga actcaatatc
     2401 agcgtggtaa tggcgaacag gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc
     2461 accagcaggc cgagcagacc tagcactgac catttcagcg cgtcgctttg ccaccaatgt
     2521 ttctttttaa tgacatccat ggggttcttc ctcattccag gacggataag gctttcacgc
     2581 cttatccgac aacaactgcc tgatgcgaca ctgatgcgtc ttatcaggcc tacatgcgtt
     2641 tcagttttgt aggccggaca aggcgttcac gccgcatccg gcatttcaca gcattacttg
     2701 gtgatacgag tctgcgcgtc tttcagggct tcatcgacag tctgacgacc gctggcggcg
     2761 ttgatcaccg cagtacgtac ggcataccag aaagcggaca tctgcgggat gttcggcatg
     2821 atttcacctt tctgggcgtt ttccatggtg gcggcaatac gtggatcttt cgccaactct
     2881 tcctcgtaag acttcagcgc tacggcaccc agcggtttgt ctttattaac cgcttccaga
     2941 ccttcatcag tcagcagata gttttcgagg aactctttcg ccagctcttt gttcggactg
     3001 gcggcgttaa taccggtgct cagcacgcca acgaacggtt tagatggttg acccttgaag
     3061 gtcggcagta ccgttacacc ataattcact ttgctggtgt cgatgttgga ccatgcccac
     3121 gggccgttga tggtcatcgc tgtttcgcct ttattaaagg cagcttctgc gatggagtaa
     3181 tcggtgtctg cattcatgtg tttgttttta atcaggtcaa ccaggaaggt cagacccgct
     3241 ttcgcgccag cgttatccac gcccacgtct ttaatgtcgt acttgccgtt ttcatacttg
     3301 aacgcataac ccccgtcagc agcaatcagc ggccaggtga agtacggttc ttgcaggttg
     3361 aacatcagcg cgctcttacc tttcgctttc agttctttat ccagcgccgg gatctcttcc
     3421 caggtttttg gcgggttcgg cagcaggtct ttgttataaa tcagcgataa cgcttcaaca
     3481 gcgattgggt aagcaatcag cttgccgttg taacgtacgg catcccatgt aaacggatac
     3541 agcttgtcct ggaacgcttt gtccggggtg atttcagcca acaggccaga ttgagcgtag
     3601 ccaccaaagc ggtcgtgcgc ccagaagata atgtcagggc catcgccagt tgccgcaacc
     3661 tgagggaatt tctcttccag tttatccgga tgctcaacgg tgactttaat tccggtatct
     3721 ttctcgaatt tcttaccgac ttcagcgaga ccgttatagc ctttatcgcc gttaatccag
     3781 attaccagtt taccttcttc gattttggcg agagccgagg cggaaaacat catcgtcgtt
     3841 aatgcggata atgcgaggat gcgtgcacct gtttttattt tcataatcta tggtccttgt
     3901 tggtgaagtg ctcgtgaaaa cacctaaacg gactctagtt tctttatacg gcaacctctt
     3961 tccatcctcc ttgcccctac gccccaccgt tgctttgtgt gatctctgtt acagaattgg
     4021 cggtaatgtg gggatgcgca cataaaatcg ccacgatttt tgcaagcaac atcacgaaat
     4081 tccttacatg acctcggttt agttcacaga agccgcgttc tcatcctccc gcctcctccc
     4141 ccataaaaaa gccagggggt ggaggattta agccatctcc tgatgacgca tagtcagccc
     4201 atcatgaatg ttgctgtcga tgacaggttg ttacaaagag agaagggcat ggcgagcgta
     4261 cagctgcaaa atgtaacgaa agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc
     4321 gatatccatg aaggtgaatt cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact
     4381 ttactgcgca tgattgccgg gcttgagacg atcaccagcg gcgacctgtt catcggtgag
     4441 aaacggatga atgacactcc gccagcagaa cgcggcgttg gtatggtgtt tcagtcttac
     4501 gcgctctatc cccacctgtc agtagcagaa aacatgtcat ttggcctgaa actggctggc
     4561 gcaaaaaaag aggtgattaa ccaacgcgtc aaccaggtgg cggaagtgct gcaactggcg
     4621 catttgctgg atcgcaaacc gaaagcgctc tccggtggtc agcgtcagcg cgtggcgatt
     4681 ggccgtacgc tggtggccga gccaagcgta tttttgctcg atgaaccgct ctccaacctc
     4741 gatgctgcac tgcgtgtgca aatgcgtatc gaaatctccc gtctgcataa acgcctgggc
     4801 cgcacaatga tttacgtcac ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc
     4861 gtggtgctgg acgccggtcg cgtggcacag gttgggaaac cgctggaact gtaccactat
     4921 ccggcagacc gttttgtcgc cggatttatc ggttcgccaa agatgaactt cctgccggta
     4981 aaagtgaccg ccaccgcaat cgatcaagtg caggtggagc tgccgatgcc aaatcgtcaa
     5041 caagtctggc tgcctgttga aagccgtgat gtccaggttg gagccaatat gtcgctgggt
     5101 attcgcccgg aacatctact gccgagtgat atcgctgacg tcatccttga gggtgaagtt
     5161 caggtcgtcg agcaactcgg caacgaaacc caaatctata tccagatccc ttccattcgt
     5221 caaaacctgg tgtaccgcca gaacgacgtg gtgttggtag aagaaggtgc cacattcgct
     5281 atcggcctgc cgccagagcg ttgccatctg ttccgtgagg atggcactgc atgtcgtcga
     5341 ctgcataagg agccgggcgt ttaagcaccc cacaaaacac acaaagcctg tcacaggtga
     5401 tgtgaaaaaa gaaaagcaat gactcaggag atagaatgat gattactctg cgcaaacttc
     5461 ctctggcggt tgccgtcgca gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc
     5521 acggctatgc acgttccggt attggctgga caggtagcgg cggtgaacaa cagtgtttcc
     5581 agactaccgg tgctcaaagt aaataccgtc ttggcaacga atgtgaaact tatgctgaat
     5641 taaaattggg tcaggaagtg tggaaagagg gcgataagag cttctatttc gacactaacg
     5701 tggcctattc cgtcgcgcaa cagaatgact gggaagctac cgatccggcc ttccgtgaag
     5761 caaacgtgca gggaaaaaac ctgatcgaat ggctgccagg ttccaccatc tgggcaggta
     5821 agcgcttcta ccaacgtcat gacgttcata tgatcgactt ctactactgg gatatttctg
     5881 gtcctggtgc cggtctggaa aacatcgatg ttggcttcgg taaactctct ctggcagcaa
     5941 cccgctcctc tgaagctggt ggttcttcct cttttgccag caacaatatt tatgactata
     6001 ccaacgaaac cgcgaacgac gttttcgatg tgcgtttagc gcagatggaa atcaacccgg
     6061 gcggcacatt agaactgggt gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc
     6121 tggttgatgg cgcatcgaaa gacggctggt tattcactgc tgaacatact cagagtgtcc
     6181 tgaagggctt taacaagttt gttgttcagt acgctactga ctcgatgacc tcgcagggta
     6241 aaggtctgtc gcagggttct ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca
     6301 acaacaacgg tcacatgctg cgtatcctcg accacggtgc gatctccatg ggcgacaact
     6361 gggacatgat gtacgtgggt atgtaccagg atatcaactg ggataacgac aacggcacca
     6421 agtggtggac cgttggtatt cgcccgatgt acaagtggac gccaatcatg agcaccgtga
     6481 tggaaatcgg ctacgacaac gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca
     6541 aaattaccct cgcacaacaa tggcaggctg gcgacagcat ctggtcacgc ccggctattc
     6601 gtgtcttcgc aacctacgcc aagtgggatg agaaatgggg ttacgactac aacggcgata
     6661 gcaaggttaa cccgaactac ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg
     6721 gtcgtggcga cagcgacgag tggaccttcg gtgcccagat ggaaatctgg tggtaatagc
     6781 aaaacctggg ccggataagg cgtttacgcc gcatccggca taaaaacagg ttgtcattat
     6841 ctgaaagggg cgaaagcccc tctgattatc gggtttagcg cgctattgcc tggctaccgc
     6901 tgagctccag attttgaggt gaaaacaatg aaaatgaata aaagtctcat cgccctctgt
     6961 ttatcagcag ggttactggc aagcgcgcct ggaattagcc ttgccgatgt taactacgta
     7021 ccgcaaaaca ccagcgacgc gccagccatt ccatctgccg cgctgcaaca actcacctag
     7081 acaccggtcg atcaatctaa aacccagacc acccaactgg cgaccggcgg ccaacaactg
     7141 aacgttcccg gcatcagtgg tccggttgct gcgtacagcg tcccggcaaa cattggcgaa
     7201 ctgcccctga cgctgaccag cgaagtgaac aaacaaacca gcgtttttgc gccgaacgtg
     7261 ctgattcttg atcagaacat gaccccatca gccttcttcc ccagcagtta tttcacctac
     7321 caggaactag gcgtgatgag tgcagagcgg ctggaaggcg ttatgcgcct gacaccggcg
     7381 ttggggcagc aaaaacttta tgttctggtc tttaccacgg aaaaagatct ccagcagacg
     7441 acccaactgc tcgacccggc taaagcctat gccaaaggcg tcggtaactc gatcccggat
     7501 attcccgatc cggttgctcg tcataccagc gatggcttac tgaaactgaa agtcaaaacg
     7561 aactccagct ccagcgtgtt ggtaggacca ctatttggtt cttccgctcc agctccggtt
     7621 acggtaggca acacggcggc accagctgtg gctgcacctg ctccggtgcc ggtgaagaaa
     7681 agcgagccga tgctcaacga cacggaaagt tattttaata ccgcgatcaa aaacgctgtc
     7741 gcgaaaggtg atgttgataa ggcgttaaaa ctgcttgatg aagctgaacg cctgggatcg
     7801 acatctgccc gttccacctt tatcagcagt gtaaaaggca aggggtaatt acgccccaca
     7861 gtgctgattt tgcaacaact ggtgcgtctc ctggcgcacc tttttttatg cttccttcct
     7921 gggatatgag cgatttttta tagtacttca cttcttcttc actaagaatg tccattattt
     7981 caatgcatta tcagagattc ttttcctttc gccggtagtg tctggacgct caggctactt
     8041 ttccaggtta ttttatttct gtattacaga ggttttatga taggtcatat cacaaattct
     8101 ggcggcaata actctttgat gaaacatgat gtggtgcaag gaaataatat agtagatctt
     8161 gatttactac gtaatttcaa tggggtgaca ggtttaaaca gagattactt tatttatatc
     8221 agtgatatat ttttaaatat aaaacaacgg aacgaaaaaa atcatgcaat aaatatgtta
     8281 cgtgaagtct caattagtaa tgatactata agtgtaaaat tctacagaaa tgaaaaaata
     8341 gaatgcgctt gtaattttct gatggataaa gatgcgcagg ggtatatcga cctgtctgat
     8401 tttgatttaa caaattgtca ttttaaaggt gacgttattt ctaaggtgtc ttttttatca
     8461 tcaaatctac aacatgtaac tttcgaatgt aaagaaattg agaattgcaa ttttactaag
     8521 gcaacagttg ataatgtcat atttaaatgt cgacgtttac acaatgtgat ttttctcaaa
     8581 acgagtggcg aatgtgttga ttttagccaa aatattcttg atacagttga cttctcgcag
     8641 agtcaacttg gtcatagtaa ttttcgcgaa tgtcagatta gaaattcaaa cttcgataat
     8701 tgttatcttt acgcttcgca cttcaccaga gcagagtttc tgtctgccaa agaaatatca
     8761 tttattaaat cgaatttgac agctgttatg tttgatcatg tgcgaatgtc gacagggaat
     8821 ttttaagatt gcattacaga acaattggaa ttaactattg attattcaga tatatttggg
     8881 aatgaagatc tcgatggtta tatcaataa
//
LOCUS       DAEBXD010000134         8898 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEBXD010000134 DAEBXD010000000
VERSION     DAEBXD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.134"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..2887
                     /locus_tag="KJL94_003549"
                     /pseudo
     CDS             1..2887
                     /locus_tag="KJL94_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KJL94_003550"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KJL94_003550"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBD4411763.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="KJL94_003551"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="KJL94_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KJL94_003552"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KJL94_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="KJL94_003553"
     CDS             complement(7280..8041)
                     /locus_tag="KJL94_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBD4411764.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="KJL94_003554"
     CDS             complement(8038..8697)
                     /locus_tag="KJL94_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD4411765.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2318 g         2049 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgta gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAEBXD010000135         8635 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEBXD010000135 DAEBXD010000000
VERSION     DAEBXD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.135"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="KJL94_003555"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="KJL94_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBD4411766.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="KJL94_003556"
     CDS             complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="KJL94_003556"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD4411767.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="KJL94_003557"
     CDS             complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="KJL94_003557"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBD4411768.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="KJL94_003558"
     CDS             complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="KJL94_003558"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBD4411769.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5259..7805
                     /gene="cyaA"
                     /locus_tag="KJL94_003559"
     CDS             5259..7805
                     /gene="cyaA"
                     /locus_tag="KJL94_003559"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBD4411770.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="KJL94_003560"
     CDS             complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="KJL94_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBD4411771.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8213..>8635)
                     /locus_tag="KJL94_003561"
     CDS             complement(8213..>8635)
                     /locus_tag="KJL94_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD4411772.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1965 a         2233 c         2220 g         2217 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     2101 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2161 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2221 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2281 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2341 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2401 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2461 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2521 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2581 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2641 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2701 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2761 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2821 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2881 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2941 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     3001 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3061 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3121 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3181 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3241 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3301 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3361 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3421 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3481 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3541 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3601 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3661 cggtggtgcg tccaatggcg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3721 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3781 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3841 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc
     3901 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3961 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4021 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4081 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4141 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     4201 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     4261 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4321 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4381 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4441 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4501 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4561 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4621 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4681 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4741 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4801 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4861 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4921 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4981 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     5041 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5101 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5161 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5221 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5281 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5341 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5401 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5461 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5521 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5581 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5641 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5701 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5761 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5821 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5881 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5941 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6001 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6061 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6121 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6181 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     6241 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6301 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6361 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6421 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6481 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6541 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6601 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6661 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6721 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6781 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6841 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6901 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6961 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     7021 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7081 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7141 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7201 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     7261 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7321 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7381 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7441 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7501 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7561 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7621 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7681 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7741 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7801 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7861 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7921 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7981 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     8041 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     8101 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     8161 ttcattgttg tatcctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8221 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     8281 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8341 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8401 cgaagcgatc gtttttagtt agcgaaccgt taactggaat aatttgcaag ctctttgacc
     8461 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8521 ggtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8581 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattg
//
LOCUS       DAEBXD010000136         8579 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEBXD010000136 DAEBXD010000000
VERSION     DAEBXD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.136"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..71)
                     /locus_tag="KJL94_003562"
     CDS             complement(<1..71)
                     /locus_tag="KJL94_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4411773.1"
                     /translation="MASVSISCPSCSATDGVVRNGKST"
     gene            129..377
                     /locus_tag="KJL94_003563"
                     /pseudo
     CDS             129..377
                     /locus_tag="KJL94_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
     gene            complement(746..1477)
                     /locus_tag="KJL94_003564"
     CDS             complement(746..1477)
                     /locus_tag="KJL94_003564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411774.1"
                     /translation="MHRLDEVVDTLLVLQKKHRIRFDVWQVVKRDHAIISFFDQGMNP
                     AVPRVAYWTPFRYPLLLNLASLFDNELAEKAWCARLEAHDGRSSSLFSEVCSELLARV
                     HTLGDRRYIELITDALSWAMTHFDELGYNCKTGKQKLQIMPNMVGFQFVLRGICSRLG
                     APNRKADIVVDQQSQFNTTQRELREFYYQIREMPWVHGPGLPVMDVTNMPAERWFSSR
                     VQKVQGWSWLIYISGSSNALWKGRS"
     gene            2263..3240
                     /locus_tag="KJL94_003565"
     CDS             2263..3240
                     /locus_tag="KJL94_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD4411775.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3525..4265)
                     /locus_tag="KJL94_003566"
     CDS             complement(3525..4265)
                     /locus_tag="KJL94_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4411776.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4386..4568)
                     /locus_tag="KJL94_003567"
     CDS             complement(4386..4568)
                     /locus_tag="KJL94_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBD4411777.1"
                     /translation="MHVAQLPSLCIFLLSLRSSLPWHYPISPKNGKPALSPRHDWLNT
                     GSLLQNIPLRIWFYPQ"
     gene            complement(4911..5218)
                     /locus_tag="KJL94_003568"
                     /pseudo
     CDS             complement(4911..5218)
                     /locus_tag="KJL94_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461876.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            5711..6880
                     /locus_tag="KJL94_003569"
     CDS             5711..6880
                     /locus_tag="KJL94_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411778.1"
                     /translation="MNQKWKTLIISLLTPSSIISGIALIVLWGYFSRLGRLDIFFDVM
                     NIKSILVLVCCATILSLAMIVFIFFITSFFIPVVIPQDINNLPAYNKIQGNFLSVMML
                     SGMFPMAFIYVLYCAFDFDQNVKDNSGWLSIASMGVLIAVILAIVNKRYLEYDLSFKN
                     NRMKLLRRVQIYLVIPLSIALLVHLQLIPLEIVFSNIATSDKSVNFKVIAELAFMSYF
                     IFLLTMLPGVIYLKMNPQHKFSKRISYSFIASLMLLLIISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAAWDKKNIKPGDYYSVQAVSMFTTNQFILLCPKD
                     IIKFYRESWKFELLNVDFDINTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSS"
     gene            6913..7041
                     /locus_tag="KJL94_003570"
                     /pseudo
     CDS             6913..7041
                     /locus_tag="KJL94_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(7076..7369)
                     /locus_tag="KJL94_003571"
     CDS             complement(7076..7369)
                     /locus_tag="KJL94_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411779.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGARTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKCISSDLI"
     gene            complement(7475..7750)
                     /locus_tag="KJL94_003572"
     CDS             complement(7475..7750)
                     /locus_tag="KJL94_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD4411780.1"
                     /translation="MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIYVLRLWHTRENR"
     gene            complement(7750..8034)
                     /locus_tag="KJL94_003573"
     CDS             complement(7750..8034)
                     /locus_tag="KJL94_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBD4411781.1"
                     /translation="MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWSDSLSTDNPLPVPR"
     gene            8265..>8579
                     /locus_tag="KJL94_003574"
     CDS             8265..>8579
                     /locus_tag="KJL94_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBD4411782.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFY"
BASE COUNT         2252 a         2010 c         1971 g         2346 t
ORIGIN      
        1 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
       61 acagaagcca caggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
      121 atcaccgtac aaatgataat gttccaggct tgctttccct gataactgca catctcaaag
      181 acctgcctga tgatggaaga aatgaagatg tgtttaaaat gttgagatca tctgccgcaa
      241 tacttcatgg gatcaataac ctgcgtaaca attacagcat ggcccatcct actgaaactt
      301 tgctgaatga agccgatgcc cgttttgcca taaatctggt tcgttccatc atgacttatg
      361 ttgatgagtt gctttagttt taataaaaat atgagactca cagcgaacag atagaacttc
      421 aacgaagaag tgaagtacgg ccagaatttc gagctctggc cctccggaag atgccggaca
      481 gacgcatcat cccgtcgtgg ccgcttccat ttttgtgtgt actcaacctg ccggatcagg
      541 atggcggcag gatttgcacc cgatgttcca gccgccttgc ctcttcctga tcccggtatt
      601 ccctcgcctt tttcatcatt tctgccgttg gctcctgcag tttgtcgaga aactccttag
      661 atcgtttagc caccgactga agggagacac tgtccgtcct gccggtattg cgattggtgt
      721 aaaccaggcg ggtaaggggg tctggttaac tccttccctt ccataaagcg tttgaagatc
      781 cagagatata tatcaaccag ctccagccct gcactttttg tacccgactg gaaaaccagc
      841 gttcagccgg catgttcgtc acatccatga ccggtaaacc gggaccgtgt acccacggca
      901 tctcacgtat ctggtaatag aactccctca gctcccgctg ggtggtgttg aactgtgact
      961 gctggtccac aacgatgtcg gctttgcgat ttggtgctcc caggcgggaa cagataccgc
     1021 gcaatacgaa ctgaaatcca accatattgg gcatgatctg cagcttttgt ttaccggttt
     1081 tgcagttata tcccagttcg tcaaagtggg tcatcgccca ggacaacgca tcggtaatca
     1141 attcaatgta acggcggtcc cctagggtat gaacgcgcgc aagcagctca ctgcagacct
     1201 cactgaacag agatgaggac cttccatcat gggcttcgag gcgggcacac cacgcttttt
     1261 cagccagttc attatcgaac aggcttgcca gattcagtag cagcgggtaa cgaaaggggg
     1321 tccagtaggc aaccctgggc actgcaggat tcatcccctg atcgaagaac gaaataatgg
     1381 cgtgatcccg tttaacgacc tgccagacat caaaccgtat ccggtgcttc ttctgcagga
     1441 caagcagcgt atcaaccacc tcatcaaggc ggtgcatgcc aagctctgcg gcgtgcagac
     1501 gctgtacttg tcaacgccac gatctttgac cgtcttatat cgacagagtt atccggcatg
     1561 gtgtcaatcc gttcacaatt tttccatcgg agcagcctac caccggtgat aaccagactt
     1621 tgcctgtagt ttgtatacac aatccctggc attgaaacac acatatgaag tgtacttcgg
     1681 atatgaccgt acgctcagga agtacggcag attccgtcaa aaattacctg taaagacaga
     1741 catcagaaaa acagtgctcg ccgcagaccg gtgaccgggc gaagcccgcg aaccggcgag
     1801 gaagcgcaat ggatctgtag ttacagtgag caggtacgcc ctgaagcctt cataatcggt
     1861 agtgtatgct gtttttctgg aatgatttat ccacatatcc ggaggcccga tccagtaaac
     1921 agatccatga atgatcaaca aaggatccat taaagatccc catatctctg caaaccttgt
     1981 cactcatggg ccgggactac gatcacataa gctgtggcat gttactgata aactgtaaca
     2041 tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg acataaacta
     2101 tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt cagtcagtta
     2161 gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc tggaggcttt
     2221 ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa gtcgtccggt
     2281 gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc gctgatgcgt
     2341 ctgggcgtct ttgtaccgac ccttaagtca ctgaagaaca gtaaaaaaaa tacactgtca
     2401 cgcaccgatg ccacggaaga actgacgcgt ctttctctgg cccgtgctga gggattcgat
     2461 aaggttgaga tcaccggccc ccggctggat atggataacg atttcaagac ctgggtgggg
     2521 atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact gccttttgtt
     2581 gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct gcgtgagcgc
     2641 atcagccctt ccctgaagcg cattgccggt accgtgatct cgttttcccg taccgatgag
     2701 aagcacaccc gggaatacat cacccatctg gtacagtcag cctactacga tactgaacgg
     2761 gatattgttc agttacaggc tgatccccgc ctgtttgaac tgtaccagtt tgacagaaag
     2821 gtccttcttc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc ccaggcactc
     2881 tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc gcggctgcgt
     2941 gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag acgggcaatg
     3001 gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg gcggacaaaa
     3061 ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga gagtaaggaa
     3121 aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg agatggcgga gaaacttgcc
     3181 ctgcttgaaa aactgggcat cacgctggat gacctggaaa aactcttcaa atcccgctga
     3241 acataaactg tagtcagtga agagcgctcc tttactgact acagcttata ttaaccggtg
     3301 cagtgagtgg tctgctcact gcagtttata ttctgtttcc tgcagtgctg cctgtggctg
     3361 agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa cctgctgctg aggcattgct
     3421 cccttcataa aacatgactt actcactaca gcttatatac atgctccagc ttatgttatg
     3481 tctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct caggggaggc
     3541 agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac tgccagcgtg
     3601 gcagccatat ccagtgcaaa cactctggta taaacctcca tcgaacgtgg atccctgtga
     3661 ccagccagtg cctggatgac tttccggggc tggcggtgat agagcatgtg catgatatag
     3721 ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtaccccgtc tgcttcggcc
     3781 cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacagc ccataatggt
     3841 tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac atagcttata
     3901 tctgtcagcg gaaccaggcg gacttcatct tttggcgggc gtccgcgtcg cgcacgcact
     3961 ttttcggaca ggatccgcac aaacggtctt actccattca ggtcaaatga ttccggcgtc
     4021 agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa cagcatatgg
     4081 tgacgctgat ccggcatata gaagagaagg gcactcactt ccggggccag caggtaggcc
     4141 ggtgtggccc ccgtggaagt ggccattctt ctcagggaga gcgctgttgc aaaatccacc
     4201 cccggcgcaa tgggtaacag ggagacgcgt tctggtgaat tctgcagggg aatgacattg
     4261 ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta ttctgacctg
     4321 aacgcccgga tccttccacg acaataaatc gcagcgcgcg gatccttttg tttgcttatc
     4381 acgcattatt gcgggtaaaa ccaaatacgt aaaggtatgt tttgcagcag ggatcccgta
     4441 ttaagccagt cgtggcgcgg gctgagggct ggtttgccgt ttttgggaga aatggggtaa
     4501 tgccagggca aagatgatcg caaggaaagg agaaaaatac acagagaggg gagttgcgct
     4561 acgtgaattc tggctaggta cacccctgta accctgccag ttttcacgca acgcaatgtg
     4621 gtggctgatt actgtcagtt gaaatgacgg ctggcatcct gatgctgact taatatacga
     4681 ataataacaa tttcagtatc agtctgcaga aaataaacca tatgccgttc aacgggtagt
     4741 gcgaatgtgt attccccagt tcaggagggg gagtaccgat attatgatta ctcataacct
     4801 gaaaaatgtc ggatatctga ttaatatatt tatcagccta ctcttcaccg aaatggtggt
     4861 acccgtaatg ctagatatcc tccagatcct gactggcttt aggtgtgagt ttaacggttt
     4921 tcaccggaag ccctcgcgcc agctttcact tttttcagaa acgcattttt ttcccacgac
     4981 acaggttcac cgctgctcaa gccttctgcc agtaaatccc gcaggacacc tggagacgtg
     5041 actctgcctg tttctctcga aggagacgaa gtgactcgcg gattacctca ctctgtgtac
     5101 gataatcacc tgattctatg agagattcta tgtactccca gagttcatct ccgagagcaa
     5161 ctgtcattgt tctggccata tcaagcctcg tgattgtaag tttaaatttt acatgcagtt
     5221 tattcgatac tcaaaggtgc ttccagtaca gaacatttgg gagaattggg gtaatgcctg
     5281 gaacagacag atacggagaa aaaccctgca gataacctta tacgacaact gaaggtgtct
     5341 gatgccatat tcttagctac ttctctgtct tcaggcggct tagtcacaac ataagaccga
     5401 tgcataataa gcatattttt tcatgttatg gtcttgttca tgcatgaact attatctatt
     5461 cagtcacttt taaagtttta tatcatactg catatgatat atatcatctc ctatattcag
     5521 actaaaaata ttatgacgat ggtcaaatga atttagtgaa aacgctacct aaaacaatat
     5581 gcttatgata taacttatca agaatatgcg acaatcttct tgaaaggtta cggagaagag
     5641 tcagataatg aagtgttata actcattcag cttttttatg ttctctttat agtaaaacca
     5701 ggagattaag atgaatcaga agtggaagac gttaattatt tcattattaa caccatcaag
     5761 tattatatca gggattgcgt tgatagttct ctggggatat ttcagtcgcc tgggccgact
     5821 agatattttc tttgatgtaa tgaatattaa aagcatcttg gtattggttt gttgtgcaac
     5881 tatactatca ttggctatga tagtatttat cttttttata acctcttttt ttatacccgt
     5941 agttattcct caggatataa ataatcttcc tgcttacaat aagattcagg gtaatttttt
     6001 atctgtgatg atgttatctg gcatgttccc catggccttt atatatgtgt tatactgtgc
     6061 atttgatttt gatcagaatg ttaaagataa ctctggctgg ttatctattg caagtatggg
     6121 tgtactgata gctgttattt tagccatagt aaataagagg tatcttgaat atgacttatc
     6181 gtttaaaaac aatagaatga aactgttgag acgtgttcag atctatcttg tcattccgtt
     6241 gtctattgct ttactggtgc atcttcagtt aattcctctg gagatagttt ttagtaatat
     6301 tgcaacttca gataagagtg ttaatttcaa agtgatagct gaactggctt tcatgtcata
     6361 ttttatcttt cttctgacaa tgcttcctgg agtcatttat ctgaaaatga atccacagca
     6421 taaattttca aaaagaatta gctattcatt tatcgcatcc ttgatgttat tattaataat
     6481 atcgactcaa ataaccgtgt taccagtgat ttttacccat tccgttatta agctatcagg
     6541 tataagtgac ttcaaaatac atagctacat tattaaaaca agtgaatatc ctgaagagtt
     6601 tttttccaat gcagcatggg ataaaaaaaa tataaaaccg ggggattatt actctgtgca
     6661 ggctgtttct atgtttacga caaaccagtt tattctcctt tgtcctaagg atattataaa
     6721 gttttatcgg gaaagctgga agttcgagtt gcttaatgta gacttcgata taaataccag
     6781 gaagaaactc caggaagaag ctgcatattg cgtaccaatt tctgcaatat ctgtaaaacg
     6841 atgggatatg cctcttcagg gctcgaaacc ctcaagttag aaggcaacta gggcgactgt
     6901 attctgtgac gaaatgaatg gctggctcac tataataatg agcgaaccca tcagggaaaa
     6961 atgtgcaatg ggcggacgcc aatggaaacg ttactcgatg gaaaacgcat ctgggccgag
     7021 aaaaatttaa gccagatgta atctgacaga tacctgtata aataaccgat aactgtcaga
     7081 tcaggtctga gctaatacat ttcaataaag gtacttgctc gcgctctgtc attttctgaa
     7141 actcttcatg ctgcatttcg attttccttc taagatcata actcgcatac ctgttctttt
     7201 tatgtatatc tatatctatt tttgtgacga gttcaaacca caatgttcgc gctccccatg
     7261 cagcgttatg aaacatttcc gcaacggcta tagagtctgt attaactgct tcagtcatgt
     7321 agtcgctaat acgttcagcg gtaattttcg ttatttcatc gtaattcatt ttatctccta
     7381 caagattatt taatggaatg caaaatatct ggtctgataa taatgttcca gaattaatat
     7441 ctgcaggcta tgttaagttg tccccacaca caatctacct gttttctcgt gtgtgccaca
     7501 gacgcaatac ataaatagtc tggccattaa tttcgtaacg gatttcgtac tcgccagcaa
     7561 aaatccgtct gacctccctg ggttcaaact gaaacaactg ttctcccata cgtggatgag
     7621 ttaacagaat gaccggtgcc tgtgtcaggg actgtaccgt tctggcggca gcaggtttac
     7681 tggccagcac cagaaaatca aataaccgcg acaaatcaga aagcgcctta ctggtccact
     7741 tcagttccat cagcgtggca ccggtaacgg attgtcagta ctgaggctgt ccgaccaggc
     7801 ctgtacagcc tgatggtcga taacctgtcc ggatgtcaca tcgtccaggg cttccagcgt
     7861 caggcgatta cgctcctcct cctgggcaag ccatgcagaa agcgcctgtt tgataaccca
     7921 gccccgggaa cgttccagtc tggcggccat ctggtcgact ttatcagcca taggtaatgg
     7981 cacatgcgcg gtaattactt ttgttgcttg tgcggcattg tttttcattt gcatatccga
     8041 ttagttgtta atcatattta atcatagtga ttactttcag acggaacaag gcaaataatg
     8101 cgcgtcagcg catgtaaggc ggcatacgga ggttacatgt agcacaggtg taaaaccgcc
     8161 gaagcccggc gtaagccggt actgattgat agatttcacc tggcactgtt gcaaatagtc
     8221 ggtggtgata aacttatcat ccctttttgc tgatggagct gcacatgaac ccattcaaag
     8281 gccggcattt tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca
     8341 tcagttaccg tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca
     8401 cgatttaccg ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg cgctggtact
     8461 ggcgtaaccc ttccgatctt tgcccgtggc acatggatga aacctacgtg aaggtcaatg
     8521 gccgctgggc gtatctgtac cgggccgtcg acagccgggg ccgcactgtc gatttttat
//
LOCUS       DAEBXD010000137         8479 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEBXD010000137 DAEBXD010000000
VERSION     DAEBXD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.137"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..121
                     /locus_tag="KJL94_003575"
                     /pseudo
     CDS             1..121
                     /locus_tag="KJL94_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(151..3063)
                     /locus_tag="KJL94_003576"
     CDS             complement(151..3063)
                     /locus_tag="KJL94_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411783.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3066..3182
                     /locus_tag="KJL94_003577"
     CDS             3066..3182
                     /locus_tag="KJL94_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4411784.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3546..4388
                     /locus_tag="KJL94_003578"
     CDS             3546..4388
                     /locus_tag="KJL94_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD4411785.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4391..5479
                     /gene="capU"
                     /locus_tag="KJL94_003579"
     CDS             4391..5479
                     /gene="capU"
                     /locus_tag="KJL94_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBD4411786.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRARVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5484..6434
                     /gene="virK"
                     /locus_tag="KJL94_003580"
     CDS             5484..6434
                     /gene="virK"
                     /locus_tag="KJL94_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBD4411787.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6499..7443
                     /gene="lpxM"
                     /locus_tag="KJL94_003581"
                     /gene_synonym="msbB"
     CDS             6499..7443
                     /gene="lpxM"
                     /locus_tag="KJL94_003581"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD4411788.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7597..8479)
                     /locus_tag="KJL94_003582"
                     /pseudo
     CDS             complement(7597..8479)
                     /locus_tag="KJL94_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2245 a         1928 c         2007 g         2299 t
ORIGIN      
        1 cccggtcagt taccgcgcac atggcctgcc cgtgtcagaa aatctgctgg agcaggattt
       61 ttacgccagt ggtccgaacc agaagtgggc gggagacatc acgtacttac gtacagatga
      121 agttcggtta catcctgtca gcacggaacc tcacgctttt tgagcggcac gacggatccc
      181 ccccacccag tatccgacag acttttcact gcgttcgtaa aaacccttct tccctcccag
      241 catatccact aattccggtg acatatcctg cagataagtc tgcaaatcat gccccagatc
      301 ttccggtgac tgcaactcat aaaccgcgcg ccacacatca cccttcctta ttttaagccc
      361 cgggttaaga atatactgtc cgtggttcag cctcagaaaa agcttttttc cataaggatt
      421 tttgctgtta acttcatgtt gagagagctg gctgctgata taactccttc gtttgcggcg
      481 ttccggcagc aacgcttcag gaagatgaac cacacactct gccagtcgtg ccgcactgaa
      541 ggcttccgaa aaaaacagag cacttcccag atgcgtgtgg aacagcgcct gcatcaggat
      601 caccagaaac attgccatct gccggttgtc cagtttgatg aggcgtcctt ccacctgcag
      661 tgacagactt tcaggtttca gcagggtata caactgcgca cttttctgtt gcgcataacg
      721 gggcgtactg accgccgctg acagaacttg atgataagcc gccagcccgt cattaccggt
      781 cagcaaggga tctgcaccac gctccagcag taactgaacc agtgcaatat tacctgcccg
      841 ggctgctgac atcagcggag taagattaaa cggcgaacgg tgatcaacac cataacgttc
      901 aatatcccgc aacacggcaa ccgggttatt cattgagtat ggccggaaat acttcagctc
      961 cagtaactgc agagtcttcg ttctgggctg ccttgtgggc ttaaaatctt cccggtacag
     1021 tgcattatac agcgggaaca gggaataaat cagactgtac tccagcgcct gcagttgtat
     1081 ggttttatcg gctgtacctg cgaggatatg ctgtttccac tgtcgtgcat cctctgcggt
     1141 gataatctgc catggcatct cagatgtctg caggatccgg ctacggatgg tgtccgcctg
     1201 ctcctgcttc ccctgttttt caagcagatg tgcggttttc tgccactcat cacggctgga
     1261 aatctcttcc tgcagattca gaggctcttc ctgatgcgtg agcgcaagca gactccacag
     1321 aggatgctcc acctgctgtt caacaagata cacattgtgt gtagcacggg tgagcgcaac
     1381 atacagcgcg ttggtaaaaa atttatagat ttccgctgaa cgatcctgcc tgtcccgcgg
     1441 acgggaataa cgggcctcgc cctcaagatc agccggtgta agaccctcgc aaatatcatc
     1501 aaatgcctgc cgggcagcgc ttactatatt gtaaaggatc accgtttcgt attccagccc
     1561 cttaacctcc tgaaccgaaa agaccagcgg cgtactgaac cagcatcgcg cctgtgcttt
     1621 ctgttccgga tgcatcacaa tgaccgctac acgattcgaa agtgaggttt tggcattcag
     1681 ttcctgcttt gtctcttccc tatcgtccag caaccggatg gtcccttctg cctgaccgca
     1741 gcttctgaca aaatggtgac tttcccgatc aatagcacta aagcggacct gtttgagtct
     1801 cagcaaccgg ttagccagtg cagtgacgtg gtgcccgtta cggtaattcg cctgcaggat
     1861 attaacggtt gtgtcgtttc cctgttgctg ccggaaaaag agacttttga gcgaactcca
     1921 tgagaaaaag ctcggatgaa ctatctgatt ggcatcacca cagagtaaaa actgaccggg
     1981 atgacgtagc gttttcagta ccagttgcag ttgaacaggc gtcatatcct gaacttcatc
     2041 aacaaaaatg acatcataac ggggagttgc caacgaaaga tactgatgac tgagcagatt
     2101 actgtcatac tgatgcgact gtttcagcca tgcgatataa cgttcaaaca ggacatagac
     2161 cgtcgggcgg tcctccatgc catagagcga ctgccgtatc cccagtgaca gatacgcctc
     2221 ccgggacagc ggcccattcc cggaggcaac cgcaccgatc acgcccctga actcctcata
     2281 cagggtatgg gcagccccca gtgcggcgat agccctgttg cggggcagcc agtcgctgaa
     2341 cgcactgaaa gtcacctccc tgccttccgg aatgcgcaga gtttccagaa attccgtcag
     2401 cgaaagaaag tcaatattct gtacctcact cccttcccct gaagcgtcat agagagtacg
     2461 cgctttttcc accagaaatg aggaaaggct gagatacagt atatcaccag cagcctgctt
     2521 cattttttcc agtaccagcg ccgtcttacc acttcccgcc gagccaacaa tcaccagcgg
     2581 tagcgggtgt tcgtacagtg cctgctgatc atcatcaaag gacagcattt tatccaggcg
     2641 gtggaaacgc ccatgagacg gattgatata cgtcagcgct tcagtcgcga tatcaacagg
     2701 atccggcact ggttgttgtt gtaagcgccc ttcatcaatc accacgtttc tgcgaagaaa
     2761 acgtgaagtg ttataggcat gattgcgaat atattccaga acaaggcaga tagtttcacc
     2821 gtggtagcga tacagcgaaa acagtaatcg gtcccggata ctgagccggg cgcggtaaag
     2881 attggtgtca attttacgaa catcagcctg aacaaagtta tcggtggcaa tagcctgtgc
     2941 aaatttttta taacccggga tcgtgtcagg attcagagta tgataaagac agattttcat
     3001 ggctgccggt ttccgtcgtg caaaaaacat gctgatttta cgaaggaatg ccttactgaa
     3061 caaccttgcc cggaataccc cccgaaatca gatggaacaa tcagaaaaat gctctgcagt
     3121 tatggaataa agaaggtggg gtggtacgcc gggagggcga tccgctggaa atggcgcagt
     3181 agctgcaggc ctgtcatgat gccggaatag aagttcgtgt acaggttaat gaaagcgtca
     3241 ccccgtaaat aaactctgcg ctgaaattcc tttttttcat tggagcacct gtgttgttct
     3301 gaggtgaaca tatcacctct gttcaggtag ccaaattcag tgtgccacct caaggttctt
     3361 agtgagtcgt ttttaaaaga tatgataata tatcattgtt tatgaattgt tgatgtttaa
     3421 tttagtaaat tatatcaaat atgccatggg tttgctatgt tattcatcca gtatctggaa
     3481 ataaaatgca ccagctttat tgggttttcc ttttgtctct gcttactagg tgccagaaac
     3541 tttaaatgtt aaacgaaggg ggcattttgt tcaaagccaa tcatgttccg gtattaatgt
     3601 atcatcatgt cagtcattgc cccggactgg ttacactttc tcccgtgaca ttccggaagc
     3661 aaataaaatg gttggctgaa aacaactgga aaacactgtc atcagatgaa ctggagtttt
     3721 tttatcgggg cggaaaactc ccccggaaaa gcgttatgtt gacatttgat gatggatacc
     3781 ttgacaactg gtttcaggta tatcctttat taaaagagtt taatctcaaa gcacatattt
     3841 ttttaattac cggttttatt gggaatggtc cggttcgtca ctctcctgga aaggaatatt
     3901 ctcatcgcga ttgcgaacat caaatagcga cagggaatgc tgataatgtt atgcttcgct
     3961 ggtctgaagt aaacgaaatg ttgcaatctg gtctggtcga atttcatgtt catacgcata
     4021 ctcatacacg ctgggataaa aagttcagtt cacgagaaga acagtgtaaa catcttcggc
     4081 aggacttgtt gagcgggaga gaatatttga aagaaatgac aggaaaatgc agtaaacatt
     4141 tgtgttggcc ggaaggttat tataacaagg attatattca ggtcgctgaa gaacttggat
     4201 tctattattt atatacaaca gaaagaagaa tgaatgctcc tgctaaagga actacacgga
     4261 taggaagaat aagcaccaaa gaacgggaaa gttgcgcatg gctgaaaaga cgactttttt
     4321 attatacaac cccgtttttt tcatcgttgc tggcctttca taaggggcca agattaccgg
     4381 atgattaata atgaatatac tatttacgga atcatcaccg aatataggtg gtcaggaatt
     4441 acaggctgtt gctcaaatga aggccctgaa gaaaatgggg cattcagttc tgcttgtctg
     4501 cagggaaaac agcaaaattg cttttgaagc cagtaaattg gggattgata tcacattcgc
     4561 gttatttcga aacagtcttc acatccctac tgcatggaga ttactcggaa tagttcatgg
     4621 ttttcagccc aatgcaatcg tttgtcacag tgggcatgat agcaatattg ttggtttagt
     4681 acggttattc actaggaaac atccattcag aattatcagg caaaagacat atttgacacg
     4741 aaaaacaaaa gttttttcaa taaatcattt ttgcgatgag gtgattgttc ccggaacaag
     4801 tatgaagaca catctggagc aggaaggatg tcgagcccgg gttactgttg tgcctccagg
     4861 ctttgatttc cagaaattat acgttgattc ccgaaacagt ttgcctccaa atgttctttc
     4921 ttggctggcg tcccgaaggg gatgccccgt tattgctcag gtaggaatgt tgcgcccgga
     4981 aaaagggcac gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt
     5041 ctgttggttg attgtggggt ctggttcgcc tgaactgcgg gagcatttac agtatcagat
     5101 tgacagtatg ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc
     5161 cgtatatcgg gttgccagtc tggtggttct gccttcagaa aacgaatcgt ttggtatggt
     5221 actggcagaa gcatcggcat tttctgtgcc tgtactggcc agtcagattg gtggaatccc
     5281 tgatgttatt cagaataacc agaccgggac attgttacca gcaggtaata agcacgcatg
     5341 gatgtgcgcc ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcca
     5401 ggcaaaacag gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac
     5461 attagcgaag cacaagtaac aatatgtttt ctgtaagtaa cttatcattt atcggtttcc
     5521 ttaaaaggat tgttttttcc tcagattcac tcccggggaa gtgggaacac agaaaattcc
     5581 ggttcatgta cattttgcga tgtgctataa atccggttgc cagtattcga tattattacg
     5641 aactgcgttc cttgcagtgc attgaggata ttctggcaat acaacccacg ttgccagcaa
     5701 gaattcatcg accttatttg cataaagggg ggcgagcctg gtcccggggg caatatattc
     5761 ttgagcatta tcgctttgtg caaaatttgc cagaaaaata ttctgaattt ttgtttccac
     5821 aaaaatcggt atcattagtc caattcatcg ggaaagatgg agaggatttt gatatccagt
     5881 gttctcccag tggttttgac cgggaaggag agttgatgtt gtcattgttt ttcaataaaa
     5941 tagtgattgc ccggttaaca ttttcagtca ttctgactca aaatggacac accgctttta
     6001 ttggcggatt acagggagcc cccaaaaata ccggacctga tgttattcgg tgcgcaaccc
     6061 gggcctgtta tgggcttttc ccaaaacgga ttatttttga agcattttgt gcgttgatga
     6121 aagcttgtaa tgtctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat
     6181 tgagatactg gtatcagaaa cgcaaaacct ttgttgctgt ctacagtgat ttctgggagt
     6241 ctgtagcagg gaaaacctgt ggtgactggt acaaattacc aacacaggtg gttcgaaagc
     6301 cgctaagcaa tatagctagt aaaaaacgct ctgagtatcg aaaacgatac gctttgctgg
     6361 attatatcca tgaaactgct atccgctctt tggatgcata tcctgtaaac tcagaacatc
     6421 aggacttaaa ttaaggtttg atgtattctc tgaataaaat attaatgatg attttggtag
     6481 gggcattcgc actaaataat gaaaaaatac aaatccgagt ttattcctga atttaagaaa
     6541 aattatcttt cccctgttta ctggtttaca tggttcgttt tgggaatgat tgcaggtatt
     6601 tcaatgtttc ccccttcatt cagagatcct gtcttggcca aaatagggcg ttgggtgggc
     6661 agattgagca gaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccggaa
     6721 aagagtgata cagaacggga aataattgtc gacaatatgt ttgccacagc attgcaatcc
     6781 atagtgatga tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc
     6841 tggaaaggcc tcgaaattct tgaggaaatt cggcacaata acagaaatgt gatttttctg
     6901 gttccccatg gctggagcgt ggatattcct gcaatgttgc tggcagctca gggggaaaaa
     6961 atggccgcca tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattctgta
     7021 cggcgtaaat tcggggggcg cttacattcc cgggaggatg ggataaaacc atttattcag
     7081 tcagtacgcc agggatactg ggggtattac cttccagatc aggatcatgg tcctgaatac
     7141 agtgaatttg ctgatttttt tgcgacctat aaagcgacat taccaattat tggacgtctg
     7201 atgaacatca gtcaggctat gattataccg cttttcccgg tttatgatga aaaaaaacat
     7261 ttcctgacta ttgaagttcg gccaccaatg gatgcatgca ttgccagcgc ggacaataaa
     7321 atgattgccc gacaaatgaa caaaacagtg gaaattttgg tggggtcaca tccggaacag
     7381 tatatctggg ttttaaaatt gttaaaaacg cgcaaatcaa acgaagcgga cccgtaccct
     7441 tgaaattata aagtacaggt atttccacta gttgtttctt acaggttacc aatcgaaaca
     7501 catccccctt ccgtgtgttg cccgtttggc acggaggact gtgtagatgc agttcgttaa
     7561 atttaattta tgcctaaata gctgcgctta ataccgctac acttttgcca gcccatgttt
     7621 gcctccgggg aatgggctga cggtttccat aaaatggcga acttttttca acagttgcca
     7681 cattgagcgg cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc atagccgttc
     7741 aacatgattc acccatggcg agtaaaccgg ctgataaatg cccctgaact tcgggttctc
     7801 cttcagccag ctctgtgttt cccggctttt gtggataatg tagttgtcca cgatcagcgt
     7861 gatggttttc gcacgacggt atgtcgcttt aagccgcttc agcaggctga tgaacagcgc
     7921 cgaacttttg ctgttgccgc ccacatagct gactttacct gtccgcgcag ttgccagtcc
     7981 gcaccgattt tgggatgaag atggatatcc acttcatctt cataaaagac cggatgctct
     8041 gcgctgcatt cgtccagtgc tttatggatt gctgccatct tttcatcttt atgcgggtca
     8101 cggatacgca gagttggcgc agcccttcgc cacacaatcc ccacagacgg caaccagcgg
     8161 cgaacggttc cggcatttaa ctggcaaccg gttatctcat tgatttttat tgccagcagt
     8221 tctgtactcc agcgtgaacg ctggtagcca aagtcgccgg gagaatgttt taccagctca
     8281 cgtaacagtg tgcagatatg ctcaaacggc cagcgacggg cacgcccggc aggtaatgat
     8341 ttcagtccct caacacccga ctgcgtgaac cagttaatcc agcgtccaac agaggagcgg
     8401 gcgcagcaga gcgttctggc aacgtcgctg acacggtcgc cccggtgcag ctgaggtata
     8461 ctggcaatag cggacacta
//
LOCUS       DAEBXD010000138         8415 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEBXD010000138 DAEBXD010000000
VERSION     DAEBXD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.138"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="KJL94_003583"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="KJL94_003583"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD4411789.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="KJL94_003584"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="KJL94_003584"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBD4411790.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="KJL94_003585"
     CDS             3115..3399
                     /locus_tag="KJL94_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD4411791.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KJL94_003586"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KJL94_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBD4411792.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KJL94_003587"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KJL94_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBD4411793.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KJL94_003588"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KJL94_003588"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD4411794.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2140 g         2003 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta gcgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gctta
//
LOCUS       DAEBXD010000139         8395 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEBXD010000139 DAEBXD010000000
VERSION     DAEBXD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.139"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            3..816
                     /gene="ttdA"
                     /locus_tag="KJL94_003589"
                     /pseudo
     CDS             3..816
                     /gene="ttdA"
                     /locus_tag="KJL94_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            813..1418
                     /gene="ttdB"
                     /locus_tag="KJL94_003590"
     CDS             813..1418
                     /gene="ttdB"
                     /locus_tag="KJL94_003590"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBD4411795.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1466..2929
                     /gene="ttdT"
                     /locus_tag="KJL94_003591"
     CDS             1466..2929
                     /gene="ttdT"
                     /locus_tag="KJL94_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBD4411796.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="KJL94_003592"
     CDS             complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="KJL94_003592"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBD4411797.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4223..4438
                     /gene="rpsU"
                     /locus_tag="KJL94_003593"
     CDS             4223..4438
                     /gene="rpsU"
                     /locus_tag="KJL94_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBD4411798.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4549..6294
                     /gene="dnaG"
                     /locus_tag="KJL94_003594"
     CDS             4549..6294
                     /gene="dnaG"
                     /locus_tag="KJL94_003594"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD4411799.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6489..8330
                     /gene="rpoD"
                     /locus_tag="KJL94_003595"
     CDS             6489..8330
                     /gene="rpoD"
                     /locus_tag="KJL94_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBD4411800.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2252 g         1862 t
ORIGIN      
        1 cagtgaataa gttgacagag attgtcgcta actttaccgc catgatttct acccgaatgc
       61 ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc cgaaacgtcg tcgatgggga
      121 aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc gattgacctg aatcgtcctg
      181 cctgtcagga caccggggag attatgttct tcgttaaagt cggttcccgc ttcccactgc
      241 ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga ggcaaccgtc aaagcgccac
      301 tacgtcacaa tgcggtagaa atttttgacg aagtaaacac cggcaaaaat accggtagcg
      361 gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga cgatgcggaa atcgaagttt
      421 acatggcagg cggcggctgc acgctacctg gccgctcgaa agtgttaatg ccgtcagaag
      481 gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc caccctcgcc gtaaacgcct
      541 gtccaccggt actggtgggc gtgggcatcg ccacctcggt ggaaaccgcc gccgtactct
      601 cgcgtaaagc cattttgcgc ccgattggca gccgccatcc caatccaaaa gcggcagaac
      661 tggagctacg cctggaagaa ggactcaacc gtctggggat tggtccacaa gggctgaccg
      721 gcaacagttc agtgatgggc gcatcgtcgc ggcacgctgc tggttcatgc cgatctcacc
      781 tttgaaaatc tgtctcacac ccggagcgcg ttatgaaaaa gatcctgaca accccgatca
      841 aagctgaaga tctgcaagat attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg
      901 tgacctgccg cgacgtttgt caccgccgtt tgatcgaact gaaacgtccg atcccttacg
      961 atctcaacgg caaagcgatt ttccacgctg gccccatcgt gcgcaaaaac ggtgacaaat
     1021 gggagatggt ctccgtcggc ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat
     1081 ttattgagca gaccggcgta aaactggtgg ttggcaaagg tggtatgggg ccgctgaccg
     1141 aagaaggctg ccagaaattc aaggcgttac atgtgatttt cccagcaggc tgcgcggtgc
     1201 tggcggcaac ccaggtagaa gagattgaag aagtgcactg gacagaactc gggatgccgg
     1261 agtcactgtg ggtctgccgg gtcaaagagt tcggcccgct gattgtctct attgataccc
     1321 acggcaacaa cctgatagcc gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg
     1381 tggaagagat ctgcgagcac gtccattaca tcaaataacc ctcccggaga ggctcacccc
     1441 tctccttttt cgcaggcata acacgatgaa accttccact gaatggtggc gatatcttgc
     1501 gccgctggcg gtcatcgcca ttattgctct acttccggtt cccgcagggc tggagaacca
     1561 tacctggctc tactttgccg tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt
     1621 gccgggtgcc gtggtggcga tggtgggtat ctccatcatc gccatactct ctccctggct
     1681 gctgttcagc cccgaacagc tcgcccagcc aggctttaag ttcaccgcca aatccctctc
     1741 ctgggcagtt tctggttttt ctaattcggt tatctggctc attttcgccg cctttatgtt
     1801 tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa
     1861 gatggggcat cgcacgctgt ttctcggcta tgcggtgatg ttctccgagc tgatcctggc
     1921 acctgtaaca ccgtccaact cggcgcgtgg tgcgggtatt atctatccca tcatccgtaa
     1981 cctgccaccg ctctatcaat cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta
     2041 catcatgtgg atggggattg ttgccgactg cgtgaccagc gccattttcc tgacggcgat
     2101 ggcacctaac ttgctgttaa ttggactgat gaaaagcgca tctcacgcca cgctgagttg
     2161 gggcgactgg ttcctcggga tgttgccgct cagcatttta ctggttctgc tggttccctg
     2221 gctggcttac gtgctgtacc cgccggtact gaagtctggt gatcaggtgc cgcgctgggc
     2281 agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct
     2341 gatggtaggc gcgctggtgc tgtggatttt cggcggtgat tatatcgatg ccgcgatggt
     2401 cggttacagc gtggtggcgc tgatgctgct tctgcgcatt atcagttggg acgacattgt
     2461 cagtaataaa gcggcatgga acgttttctt ctggctggcc tcgcttatca ccctcgcgac
     2521 cggactcaac aataccggtt ttattagctg gtttggcaaa ctgttagcag gcagcttaag
     2581 tggttattcg ccgacgatgg tgatggtgac gttgattgtg gtgttttatc tactgcgcta
     2641 ctttttcgcc agcgccacgg cgtatacctg cgccctcgca ccgatgatga tcgccgccgc
     2701 gctggcgatg ccggaaatcc cgctgccggt gttctgcctg atggttggcg cggcaattgg
     2761 tctggggagc attcttacac catacgccac cggacccagt ccgatttact acggtagtgg
     2821 ttatctgcca acggcggatt actggcgact gggggcgatt tttgggctga tattcctcgt
     2881 gttgctggtg attaccggct tactgtggat gcccgtggtg ttgctttaag tgaggacggt
     2941 gccggaaatt gctctccggc acccaatagt tttacgcagc cggtaactcc gccagcggcc
     3001 agcgcggacg cacgctaaca ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc
     3061 cggcataggc gatcatcgcg ccgttatcag tacaaaattc cggacgcgcg tagaacactt
     3121 cgccgcggcg ttttttcatc atttcagcca gcttcgcccg taacgtgcgg ttagcactca
     3181 cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa
     3241 tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt
     3301 cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga
     3361 aatccagccc cggacggtcg gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct
     3421 gagccgccat tttcgacagt aacggtccgc caggataatc cagccccagc agcttcgcgg
     3481 ttttatcaaa cgcttcaccg gcggcatcat cgatagactc gccgagcagc tcgtactgac
     3541 caatgccagt cacgctgatt aactgcgtat gaccgccgga caccagcagc gcgacaaacg
     3601 gaaattccgg cgggttatct tccagcatcg gcgctaacag atgcccttcc atatggtgta
     3661 ccgggattgc cggaacgttc caggcaaacg ccagagaacg ccccacggtt gcgccaacca
     3721 gcagtgcgcc gactaatcca gggcctgcgg tataggccac agcatcaatg tcttttgccg
     3781 ttaaaccaga ctcctttagc gccgcctgga tcaacggtac ggttttacgc acatgatcgc
     3841 gggaggccag ttcaggcacg acgccgccgt agtcagcgtg caatttcacc tgactataca
     3901 attggttggc taacaaacct ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg
     3961 aagtttcaat acccagtaca cgcatgactt gttttacctc gctttattac cgcgcagtgt
     4021 aggaccaatg cgggttgatg taaaactttg ttcgcccctg gagaaagcct cgtgtatact
     4081 cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt gctttacaaa gcagcagcaa
     4141 ttgcagtaaa attccgcacc attttgaaat aagctggcgt tgatgccagc ggcaaaccga
     4201 attaatcaaa ggtgagaggc acatgccggt aattaaagta cgtgaaaacg agccgttcga
     4261 cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg
     4321 tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag cgcgctaaag cttctgcagt
     4381 gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt
     4441 ccccgagagc gttgctctcc gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa
     4501 ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat cggggcctat ggctggacga
     4561 atcccacgcg tattcattaa tgatctgctg gcacgcactg acatcgtcga tctgatcgat
     4621 gcccgtgtga agctgaaaaa gcagggcaag aatttccacg cgtgttgtcc attccacaac
     4681 gagaaaaccc cgtcattcac cgttaacggt gagaaacagt tttaccactg ctttggatgt
     4741 ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg acaagctcga gttcgtcgaa
     4801 acggtcgaag agctggcagc aatgcacaat cttgaagtgc catttgaagc aggcagcggc
     4861 cccagccaga tcgagcgcca tcagaggcaa acgctttatc agttgatgga cggtctgaat
     4921 acgttttacc aacaatcttt acaacaacct gttgccacgt ctgcgcgcca gtatctggaa
     4981 aaacgcggat taagccacga ggttatcgct cgctttgcga ttggttttgc gccccccggc
     5041 tgggacaacg tcctgaagcg gtttggcggc aatccagaaa atcgccagtc attgattgat
     5101 gcggggatgt tggtcactaa cgatcaggga cgcagttacg atcgtttccg cgaacgggtg
     5161 atgttcccca ttcgcgataa acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc
     5221 aacgataccc ccaaatacct gaactcgccg gaaacagaca ttttccataa aggccgccag
     5281 ctttacggtc tttatgaagc gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc
     5341 gaaggctata tggacgtggt ggcgctggcg caatacggca ttaattacgc cgttgcgtcg
     5401 ttaggtacgt caaccaccgc cgatcacata caactgttgt tccgcgcgac caacaatgtc
     5461 atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg catggcgagc gctggaaacg
     5521 gcgctgcctt acatgacaga cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa
     5581 gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag
     5641 gcgatgccac tctccgcatt tctgtttaac agtctgatgc cgcaagttga tctgagtacc
     5701 cctgacgggc gcgcacgttt gagtacgctg gcactgccat tgatatcgca agtgccgggc
     5761 gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca aattaggcat acttgatgac
     5821 agccagcttg aacgattaat gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg
     5881 cagctaaaac gcacgaccat gcgtatactt atagggttgc tggtgcaaaa tccagaatta
     5941 gcgacgttgg tcccgccgct tgagaatctg gatgaaaata agctccctgg acttggctta
     6001 ttcagagaac tggtcaacac ttgtctctcc cagccaggtc tgaccaccgg gcaactttta
     6061 gagcactatc gtggtacaaa taatgctgcc acccttgaaa aactgtcgat gtgggacgat
     6121 atagcagata agaatattgc tgagcaaacc ttcaccgact cactcaacca tatgtttgat
     6181 tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg agcgcacgca tggtttaagc
     6241 aacgaagaac gcctggagct ctggacatta aaccaggagc tggcgaaaaa gtgatttaac
     6301 ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc cgcactgaga ggcagcggca
     6361 aatatataag tacgccctcg taattatcgt tggcggtaaa caaccgttgg atttcagcgt
     6421 taacggctga aggacatcgg gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat
     6481 accgtcttat ggagcaaaac ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg
     6541 agcaaggcta tctgacctat gccgaggtca atgaccatct gccggaagat atcgtcgatt
     6601 cagatcagat cgaagacatc atccaaatga tcaacgacat gggcattcag gtgatggaag
     6661 aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg
     6721 aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat cgggcgcacg actgacccgg
     6781 tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg
     6841 acatcgctaa gcgtattgaa gacgggatca accaggttca atgctccgtt gctgaatatc
     6901 cggaagcgat cacctatctg ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc
     6961 tgtccgatct gatcaccggc tttgttgacc cgaacgcaga agaagatctg gcacctaccg
     7021 ccactcacgt cggttctgag ctttcccagg aagatctgga cgatgacgaa gatgaagacg
     7081 aagaagatgg cgatgacgac agcgccgatg atgacaacag catcgacccg gaactggctc
     7141 gcgaaaaatt tgcggaacta cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga
     7201 aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa actgtctgaa gtattcaaac
     7261 agttccgcct ggtgccgaag cagtttgact acctggtcaa cagcatgcgc gtcatgatgg
     7321 accgcgttcg tacgcaagaa cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc
     7381 cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg
     7441 cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc
     7501 atcgcgccct gcaaaaactg cagcagattg aagaagaaac cggcctgacc atcgagcagg
     7561 ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag
     7621 agatggttga agcgaactta cgtctggtta tttctatcgc caagaaatac accaaccgtg
     7681 gcttgcagtt ccttgacctg attcaggaag gtaacatcgg cctgatgaaa gcggttgata
     7741 aattcgaata ccgccgtggt tacaagttct ccacctacgc aacctggtgg atccgtcagg
     7801 cgatcacccg ttctatcgcg gatcaggcgc gcaccatccg tattccggtg catatgattg
     7861 agactatcaa caaactcaac cgtatttctc gccagatgct gcaagagatg ggccgtgaac
     7921 cgacgccgga agaactggct gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc
     7981 tgaagatcgc caaagagcca atctccatgg aaacgccgat cggtgatgat gaagattcgc
     8041 atctggggga tttcatcgag gataccaccc tcgagctgcc gctggattct gcgaccaccg
     8101 aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct gaccgcgcgt gaagcaaaag
     8161 ttctgcgtat gcgtttcggt atcgatatga acaccgacca cacgctggaa gaagtgggta
     8221 aacagttcga cgttacccgc gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac
     8281 tgcgtcaccc gagccgttct gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc
     8341 ggatcaggcg ttacgccgca cccggcacta gtctctctgc acaaacgcca ccttt
//
LOCUS       DAEBXD010000140         8378 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEBXD010000140 DAEBXD010000000
VERSION     DAEBXD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.140"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..1719
                     /gene="espL4"
                     /locus_tag="KJL94_003596"
                     /pseudo
     CDS             1..1719
                     /gene="espL4"
                     /locus_tag="KJL94_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(1688..3425)
                     /gene="aceK"
                     /locus_tag="KJL94_003597"
                     /pseudo
     CDS             complement(1688..3425)
                     /gene="aceK"
                     /locus_tag="KJL94_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(3609..4912)
                     /gene="aceA"
                     /locus_tag="KJL94_003598"
                     /pseudo
     CDS             complement(3609..4912)
                     /gene="aceA"
                     /locus_tag="KJL94_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(4942..6543)
                     /gene="aceB"
                     /locus_tag="KJL94_003599"
                     /pseudo
     CDS             complement(4942..6543)
                     /gene="aceB"
                     /locus_tag="KJL94_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(6812..7741)
                     /gene="metA"
                     /locus_tag="KJL94_003600"
     CDS             complement(6812..7741)
                     /gene="metA"
                     /locus_tag="KJL94_003600"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBD4411801.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7898..8341
                     /locus_tag="KJL94_003601"
     CDS             7898..8341
                     /locus_tag="KJL94_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBD4411802.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2130 a         2053 c         2077 g         2118 t
ORIGIN      
        1 acacattgct gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca
       61 agatgattac tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca
      121 gactttggcg ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt
      181 cgggctagtt atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc
      241 agccatatta ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac
      301 tgaaggatgt acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat
      361 aagaattatg agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa
      421 tgtagattat agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat
      481 aattccagca cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac
      541 ccctccatta tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact
      601 cacgcaactg atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac
      661 tgcctataat agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga
      721 tatcgttgag actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa
      781 aaaaaatctc atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt
      841 agcgatatca cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact
      901 ggccacaaca catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag
      961 aacgtccagc catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat
     1021 ttttttggtc gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact
     1081 cgatctcctg aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa
     1141 tggacatagc gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat
     1201 taacatttca gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac
     1261 gggtttgttt atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc
     1321 attacccact ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg
     1381 gcaaataatt ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac
     1441 gttgtagaga cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct
     1501 gaagatatta tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg
     1561 gcttttgaat ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg
     1621 gctgaaagct ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa
     1681 gctttactca aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg
     1741 cataaacatc ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag
     1801 cgcggaacag gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata
     1861 gccagtggcg aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc
     1921 tggcaagttc gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca
     1981 tgtagcaaat ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt
     2041 ttttaaacag catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac
     2101 cgtattcttc aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga
     2161 gcggcaccat ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg
     2221 gtgatttttt ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc
     2281 ttctccagca caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc
     2341 tctttcacca gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg
     2401 aacttgtctt tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc
     2461 accattccac gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg
     2521 agatattcgc ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat
     2581 tcagcagtgg ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc
     2641 ggtgcataaa ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc
     2701 gtcgtcaggc aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag
     2761 aagggcaacg tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg
     2821 taaaacagtt cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt
     2881 tccgtcagat ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc
     2941 agcggcaggt cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag
     3001 tctttcgcca gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata
     3061 aaaagccgct cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa
     3121 aagctctccg caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta
     3181 acgcgcagta aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc
     3241 acgaccagac caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca
     3301 gcatgccagt cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga
     3361 ccatactgag cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc
     3421 ggcatttagc gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc
     3481 agcctacgtt cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc
     3541 gtgcaccgat tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg
     3601 taggttgctt agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg
     3661 ccgccctgaa taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga
     3721 gatacgaagg tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca
     3781 tagtgcttca taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac
     3841 atgctgtgga tacctgccag ggtgatgaac tggaacttgt agcccatatc cgacagctgc
     3901 tgctggaagc tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag
     3961 cagttataag ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc
     4021 gccagttcca gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac
     4081 gccaggccac ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg
     4141 gtacgctcgc cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc
     4201 gccgcatcag catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc
     4261 agacgcgccg cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc
     4321 atgtgaccgc atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg
     4381 gcttcaatca tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc
     4441 gcatcggcaa cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc
     4501 acggaccatt ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct
     4561 ggcaccgagt ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg
     4621 tccgccgcta cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc
     4681 agcgcctgac cgccagtcag tgcaccgagg ctgttgatgt agcttttttc gactcaccgt
     4741 gcagcagacg ccacattttc gctgcgccca gttgcgccag cgtgcattca ggattgactg
     4801 aaccgcgtaa tttcaccaca tcttccgcac tgtatgggcg agtaatgcct tcccaacgcg
     4861 gttgagtcca ctctttctgt aattcttcaa tttgttgtgt acgggttttc atgtgcagat
     4921 gctccatagt tatgtggtgg tttacgctaa caggcggtag cctggcaggg tcaggaaatc
     4981 aattaactca tcggaagtgg tgatctgttc catcaagcgt gcggcatcgt caaaacggcc
     5041 ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact ttcatctctt cgcccagcat
     5101 ctggcggaac aaggctttgg tcaccggttt gccattgctc aacgtttttt gatgatggat
     5161 ccactgccag atcgaggtac gggaaatttc agccgtcgcc gcatcttcca tcaggccata
     5221 aatcggcacg cagccgttgc cggagatcca cgcttcgatg tactgcacag ccacgcgaat
     5281 gttagcgcgc ataccttctt cggtgcgttc accatcacaa ggtgccagca gctgatcggc
     5341 agtaatcggc gcgtcttgtt cgcgcaatac ttcaagctga tttttacggg agccgagaat
     5401 gtcgttgaat accgccattg ccgtgtccgc aaggcctggg tgagcgatcc atgtgccatc
     5461 gtgaccgtta ttggcttcca gcgctttatc cgcttttact ttgttgagca cctggttatt
     5521 acgctcttca tctttgctcg gaataaacgc ggccatgccg cccatcgcaa aagcaccgcg
     5581 tttatggcag gttttaatca gcaggcgtga gtaagcattc aggaatggtt tatccatcgt
     5641 cacggcctgt ctgtctggca ggacacgatc gggatagttt ttcaacgttt tgatatagct
     5701 gaagatgtaa tcccagcgac cgcagttcag accaacaata tggtcgcgca gcgcgtgaag
     5761 gatttcgtcc atctggaaca cggcgggcag cgtttcaatc agcaacgtcg ccttgatggt
     5821 gccgcgcggc agattaaagc gatcttctgc atagctgaag acttcgctcc accaggccgc
     5881 ttcctgctag gactgggttt tcggcagata gaaataggga ccgctgccct ttgccaacag
     5941 tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac aggctaccgg ggattgcctc
     6001 accacgccag gtgacatgtt tttccggcaa gtgcagaccg cgtacccgac aaatcaaaac
     6061 cgctggattg ggcttgagct gataaatttt gcctgcttca ttggtatagc tgatggtacc
     6121 gttaaccgca tcgcgcaggt taatttgccc gtcgatcact ttgttccagt ctggtgccag
     6181 tgaatcttcg aaatcggcca taaagacttt cacattggcg ttcagtgcgt tgatcaccat
     6241 cttgcgctct accgggccag ttatctctac gcggcggtct tctaagtccg caggaatccc
     6301 gcgaattttc caatcagcat cgcgaatgga agccgtttcc gaaataaaat caggcaacgt
     6361 tccgttatca atatcttgct gctgctgaat gcgcgctgcc agaagtttat tgcgttgtgg
     6421 cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct tcggcagtaa gaatttgctt
     6481 ctcctgctcg ccatacggcc ttgtgaaagc cagttcatcg gttgttgttg cctgttcagt
     6541 catcgtgcag ctcctcgtca tggatccgaa aacttcccct ctgtgaacga aggaacgttg
     6601 tgcgcttttc gttcagcaca actaagacta ctcatttaaa atgcaaaatc aaaaacaatt
     6661 tccattttaa ttaaaaatac ttgataactt attgataaca atatgaataa aatttattca
     6721 cttgctgagg tgcgtttcgg aaataaaaaa ggcacccgaa ggtgcctgag gtaaggtgct
     6781 gaatcgctta acgatcgact atcacagaag attaatccag cgttggattc atgtgccgta
     6841 gatcgtatgg cgtgatctga tagacgtaat agttgagcca gttggtaaac agcaaattac
     6901 cgtgactacg ccagctcgct cgcggtttgt tttgcggatc gttgtgcggg aaatagttat
     6961 acggtacatc cgggtctagt ccggcttcca catcgcgtaa atattcctgc gccagcgttt
     7021 gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg cttatctttg ctggcaaaca
     7081 gatatgcatc cccttcttcc gtctctgcca gaatttccag atcggtgtaa tcacgaatca
     7141 acgctgccgg aaagtcagca tagcgcgaat gcggtgccag gaatgaatca tcaaagccac
     7201 gcgtcagaag cgcatgagga tggagaatat gatgctcgta aacgccagag agtttgtcgg
     7261 tgcgagtttg cttaggaatg ccgtagagga tattgagcgc ggcctgtacc gcccagcaga
     7321 caaacagcgt cgaggtgacg tgatctttcg accactccag cacctgtttg atctgcggcc
     7381 agtaagcgac atcattaaac tccaccaggc ccagcggcgc accggttaca atcagaccgt
     7441 caaagttctg ttcctgaata tcttcaaagt tacagtagaa gttgttcaga tgctctgcgg
     7501 gcgtgttgcg cgattcacgg gaatcgatgc gcaacagctg aatatcgacc tgcaaaggtg
     7561 agtttgaaag caggcgcaga aactgatttt cagtttcaat cttcttcggc atcaggttaa
     7621 ggatcagaac cttaagtgga cgaatttcct gaccagacgc acgagaagtt gtcatcacaa
     7681 agacgttttc ttcacgcaag aaattgacgg cgggtagctc gtccggcaca cgaatcggca
     7741 taacctgatt acctcactac atacgcttat acgtttagac atccagatag ctgaagataa
     7801 ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg agaaagtttc aaggaaaagg
     7861 ctacgttaga atataagaat gacgaaaagg agagaaaatg gttattagta ttcgccgctc
     7921 acggcatgag gaaggggagg aactcgttgc gatttggtgt cgatctgtcg atgccaccca
     7981 cgatttttta tcagcagagt atcgggccga gctggaagag ctggtgcgtt cctttctgcc
     8041 ggaagcaccg ttgtgggtcg cggttaatga acgggagcag ccggttgggt ttatgttgtt
     8101 aagtgggcag catatggatg cgctgtttat cgatcctgat gtgtgcggct gcggcgtggg
     8161 tcggatgctg gtggagcatg cgctctcgat ggcaccggaa ctgacaacca acgttaatga
     8221 gcaaaatgag caggcggttg ggttctataa gaaggtgggt tttaaggtta cgggacgctc
     8281 tgaggtggac gatttgggga aaccgtatcc gttgctgaat ctggcgtatg tgggggaata
     8341 agccgcccct gccgtgcctg cccaagctgg agaagacg
//
LOCUS       DAEBXD010000141         8359 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEBXD010000141 DAEBXD010000000
VERSION     DAEBXD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.141"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            69..317
                     /locus_tag="KJL94_003602"
     CDS             69..317
                     /locus_tag="KJL94_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBD4411803.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            382..750
                     /locus_tag="KJL94_003603"
     CDS             382..750
                     /locus_tag="KJL94_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD4411804.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            844..1497
                     /gene="nfsB"
                     /locus_tag="KJL94_003604"
     CDS             844..1497
                     /gene="nfsB"
                     /locus_tag="KJL94_003604"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBD4411805.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1605..2852
                     /gene="ybdG"
                     /locus_tag="KJL94_003605"
     CDS             1605..2852
                     /gene="ybdG"
                     /locus_tag="KJL94_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBD4411806.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIRRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2920..4296)
                     /gene="pheP"
                     /locus_tag="KJL94_003606"
     CDS             complement(2920..4296)
                     /gene="pheP"
                     /locus_tag="KJL94_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBD4411807.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4397..7540)
                     /gene="cusA"
                     /locus_tag="KJL94_003607"
     CDS             complement(4397..7540)
                     /gene="cusA"
                     /locus_tag="KJL94_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBD4411808.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7552..8070)
                     /gene="cusB"
                     /locus_tag="KJL94_003608"
                     /pseudo
     CDS             complement(7552..8070)
                     /gene="cusB"
                     /locus_tag="KJL94_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2043 a         2326 c         2032 g         1958 t
ORIGIN      
        1 taagccggga attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta
       61 acaatgttat gaaacaccct ttagaaacct tgaccaccgc agcaggcatt ttgctgatgg
      121 ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg gcacaaaaac
      181 tggtgaccat gttccatctg atggatctta gccagcttta cactttattg ttttgtctgt
      241 ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt
      301 tctcgctggc ggattaaacg tcgattatcg ccgaacttcg cggcatactt tgcttattct
      361 ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgccta
      421 gctcttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt
      481 aaaattggcg gcaagatttt tatgctgtcg tcggagctac gcggcgtccc ctttatcaat
      541 ctgaagtccg atccacaaaa atccctgtta aatcagcaaa tatacccaag cattaagcca
      601 gggtatcaca tgaataaaaa gcactggatt tcggtgtatt ccggcgagga aatctccgaa
      661 gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa
      721 cgcgatcaaa aaagagtgcg tccaggctaa agcgggaatc tatagcgcat ttttctcgct
      781 taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattacttac acatggagtc
      841 tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagc
      901 aaaaaactta ccccggaaca ggccgagcag atcaaaacgc tactgcaata cagcccatcc
      961 agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt
     1021 gttgccaaat ccgctgccgg taattatgtg ttcaacaaac gtaaaatact tgatgcctcg
     1081 cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt
     1141 gaccaggaag atgccgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa
     1201 ggtcgcaagt tcttcgccga tatgcaccgt aaagatctgc atgatgatgc agagtggatg
     1261 gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc gctgggtctg
     1321 gacgcggtgc ccatcgaagg ttttgacgcc gcaatcctcg atgcagaatt tggtctgaaa
     1381 gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt
     1441 aacgctacgc tgccgaaatc tcgtctgccg caaaacacca ccttaaccga agtgtaattc
     1501 tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt gcataaccct
     1561 gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag gatttaatat
     1621 cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg gtgatgattt
     1681 tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg gtggtactgc
     1741 ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc attacccaga
     1801 ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat attcaggcgg
     1861 tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc gcgcagttgt
     1921 ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt ttgaatctgg
     1981 cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag gggattaaac
     2041 tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt cagtcgccag
     2101 cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta tttaaagatc
     2161 cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg aaactgggcg
     2221 actggctgga aatgccgaaa tacggcgcgg atggcgcggt gatcgatatt gggttaacca
     2281 ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg tctctggttt
     2341 ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt attaggcgca
     2401 gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg caacgtctga
     2461 ataaagcgca tttgttaaag ccttatttaa ccagccgcca tcaggaaatt aatgagtgga
     2521 atcgccagca aggttctacg gagtcggtat taaacctgcg ccgaatgacc aatattggaa
     2581 cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt aaagatatga
     2641 ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag atctacgcgt
     2701 ttactaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata ttcgatcaca
     2761 tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc ggcaatgata
     2821 ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca tcgaatggat
     2881 gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac gcagcgtttt
     2941 aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca agcgcatatc
     3001 atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcgatgcaaa gatagttgcc
     3061 gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca tcgccgcgcg
     3121 aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcaacgttg ctaccaccag
     3181 cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaaca ccaccagcga
     3241 agtgatcgct ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc tgacgcgagt
     3301 caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc tgttagagta
     3361 aaccccgctg ttatacaccg acagcgatgc cactagaatg acgaagttca gcgcagaagc
     3421 taccacattg ctgtcgagat tatggaaaat catcacaaac gggctactgt tggatttcac
     3481 ttccacccac ggatagagtg ccagtaaaac caccagtgaa ccgatataaa acagcaggat
     3541 gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc gcgcttcagc
     3601 ggctgtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg ccagcgacaa
     3661 aatcagccca ttccagccgg tggcgaagaa accaccgtag cgccagaggt tgtcgatact
     3721 ggctttctcg ccgccgtaac cagaaaacag cagccacagg ccaaagccga tcataccgat
     3781 gatcgccagc actttaatca gcgcaaacca gaactcggtt tcgccatata agcgcacgtt
     3841 caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac
     3901 atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca ttcccaccag
     3961 cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc cccagtattt
     4021 ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct cgccaagctg
     4081 gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc
     4141 cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg cgccacccag
     4201 cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgattcga
     4261 cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt
     4321 gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta
     4381 aacgaggtat cctgctttaa ttccgtaccc gatgtcggtg cagccacatc agcttatacg
     4441 ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca atcatcggtg
     4501 cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac
     4561 cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat
     4621 acaacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac gacggctcgg
     4681 cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgaccccgg
     4741 caagggcaat aaagccggta cccgtcgcca cagaaagatg aaagcccatc caccacagta
     4801 accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca
     4861 cccgacggaa tgccagatac aacagcacga agatgatcat cagtgtcatc ggcaccatga
     4921 gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg
     4981 tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga accaccgaca
     5041 ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt
     5101 tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct
     5161 gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg cgccagcttt
     5221 gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg ccaaccatcg
     5281 ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgtg
     5341 cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc cgctcagcaa
     5401 gcgcagaagc tacgcctggc accgttcgcg ccacttcttc aatttgctca gccatcgcgt
     5461 cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg
     5521 agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagac
     5581 gcacggtgtt atccagttcc tcaatgattt tgtccattgt catgcctggc cgccactgat
     5641 cctgtggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa tcggtggcgg
     5701 tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct
     5761 tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg
     5821 gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga
     5881 gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt ttcggccagt
     5941 gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcggattac
     6001 tgctttccgg cggaattttg ccacggatcc agtaacccat caggataggg atcactacga
     6061 tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac gggccaaaca
     6121 gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg ataatcagca
     6181 gactgataaa cagcgccggc cccacttcaa cagacgcatc ggtgatcacc tgccagcgcg
     6241 ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg
     6301 cattctcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc gcaatgccac
     6361 ccagcgacat aatattggca ttcagcccct ggaagggcat gacaataaaa gcaatacaca
     6421 accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg
     6481 cgcagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg
     6541 cccggtcaat gagctggctg cggtcgtatg ttgtaactat ctccacgcct tccggcagac
     6601 tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc
     6661 cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttagt tcggcaatgc
     6721 cccggcgcat cttcgggcca atctggacct tcgcaacatc gcgcagataa acgggcacgc
     6781 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt agatagccgc
     6841 tggcgcgcac catatattct gcttccgcca gttcgattga cgaaccgccc gcttcctggt
     6901 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct
     6961 gaggatcgat aaccacctga tactctttca ccacaccgcc taccgacgcc acttccgcaa
     7021 cgtcaggaat ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg
     7081 caagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
     7141 tggcatctgg tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
     7201 ggtactccag cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat
     7261 acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca
     7321 acatggtggt ggttagcgga taagtcacct gattttcaac gatttgcggt gcctggccgg
     7381 gatagctggt tttaataatc acctgcacat cggagagatc cggcaacgca tccactggcg
     7441 tattaatgat ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa
     7501 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
     7561 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
     7621 aatcaggaac aggccgccgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
     7681 ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc
     7741 atcaacggta atcacccgct gttcgttgcc ggtatcaatc agcgcctgtg acggaatgag
     7801 cagcatcggt tcgctggcgg tgttgagttg caaccaggcg ttcatgcccg gttttagcgc
     7861 ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
     7921 aggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgcgccg gaacggtgag
     7981 ggtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg cagcagtgac
     8041 ccacaccggg tccataccct gaattcgcga ttacgcgcaa tccccctgag aaccgccatg
     8101 aacaagggtt tcatgatttg tgattttagt ttggtacgca atttggtaca caacactatt
     8161 ttcacttcag cgggtagtca tcaaactcac cataacgagc atcgttgatg atgtatgtga
     8221 tcaccacaaa tatcacatct ggactagttc cgtcttcatg cgcaggtaga ctggccccct
     8281 gaatctccag acaaccagta tcacttattt aagtgatagt cttaatacta gtttttagac
     8341 ttgtcattgg agaacagat
//
LOCUS       DAEBXD010000142         8166 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEBXD010000142 DAEBXD010000000
VERSION     DAEBXD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.142"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..983
                     /locus_tag="KJL94_003609"
     CDS             <1..983
                     /locus_tag="KJL94_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD4411809.1"
                     /translation="DRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYD
                     FSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDD
                     SNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIA
                     PYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMV
                     ICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGE
                     ENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1137..2786)
                     /locus_tag="KJL94_003610"
     CDS             complement(1137..2786)
                     /locus_tag="KJL94_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBD4411810.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2937..4286)
                     /gene="ghxP"
                     /locus_tag="KJL94_003611"
                     /gene_synonym="yjcD"
     CDS             complement(2937..4286)
                     /gene="ghxP"
                     /locus_tag="KJL94_003611"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBD4411811.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4992..5456)
                     /gene="soxR"
                     /locus_tag="KJL94_003612"
     CDS             complement(4992..5456)
                     /gene="soxR"
                     /locus_tag="KJL94_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBD4411812.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5542..5865
                     /gene="soxS"
                     /locus_tag="KJL94_003613"
     CDS             5542..5865
                     /gene="soxS"
                     /locus_tag="KJL94_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBD4411813.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5868..7445)
                     /gene="pdeC"
                     /locus_tag="KJL94_003614"
                     /pseudo
     CDS             complement(5868..7445)
                     /gene="pdeC"
                     /locus_tag="KJL94_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7874..8155
                     /locus_tag="KJL94_003615"
     CDS             7874..8155
                     /locus_tag="KJL94_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBD4411814.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2273 a         2090 c         1864 g         1939 t
ORIGIN      
        1 acgatagaat tacgagaaga gtttacctta gtaaagaaaa ggatgtatcc attaaggtgg
       61 tatattttat aaataatgtc gccgtccata ataatactat cgaaattcca cagatagtaa
      121 atggtggtta cgatttttca caccttagcc tgaagggtat cgtgattaaa gatgaagatt
      181 tatccaattc gaattttgca ggttgcagac tacaaaacgc tattttccag gactgtaata
      241 tgtataaaac gaatttttat tacgccataa tggaaaaaat actttttgat aattgtattc
      301 tcgatgactc aaatttcgct cagataaaaa tggccgacgg aactctaaat gcatgctccg
      361 ctatgcatgt tcaattctac aatgcagcaa tgaatagagc caatattaaa aataccttcc
      421 ttgactattc aaatttttat atggcgtaca tggctgaggt aaatctttat aaagtaatag
      481 cgccatatgt taatttattt aaagccgacc ttagtttctc taaactcgat ttaattaact
      541 ttgaacatgc tgatctgtct cgcgtcaatc tgaacaaagc aatcctccag aatataaact
      601 taattgatag caaactcttt tgtacgtggc tgacaaatac attcctcgaa atggttatat
      661 gtaccggctc taatatggct aatgttaatt ttaataatgc caatttaagc aactgccatt
      721 tcaactgttc tattttaaca aaagcctgta tgtttaatac ccgtctctat cgggttaatt
      781 tcgatgaggc tagcgtccag ggaatgggca tttccattct ccgtggggag gaaaatatcc
      841 ccattgatag tgataccctg gtaacactac agaaattctt tgaagaagat tgtacctctc
      901 atactggcat gtcacaaact gaggataata ttaatgcagt cgctatgaag attactgcag
      961 atattatgca acacgcagat tgaggttaag tcgaagaaca gccaatgaat tgactttaca
     1021 agagagataa cttatttgtt ttagaatcat gaatgttcgc ccgagctaac ctgttaaagg
     1081 tgcaaaagat tgcacctttt tcagattacc tcgggcctgg ttacgtcgct ttcggcttac
     1141 tgattttcct caattagcaa cgcttcaagc aaatcgagat cgtgcagtaa tttttgcagc
     1201 gtttcgttgc tgatctcccg cgtggcgcgc aggtggtaca gttcagcacg ttcagaacgc
     1261 aatgccgcca gacggaagcg acgctcaagg ttctcttcct gcacggaact ttcaacgtcg
     1321 ttacgtccat cggcgcgacg acgcaggtta ccaatgacgc gagaactgac ctccgtaagc
     1381 aactggttat cgatattctc ttcggtatcc gccgccagac gctcctccat tttctggatc
     1441 gccacaatcg ccacttctgc cgttgccgct cgcgcaatac gttcttcttt caattgttgc
     1501 gaatggtccg cgacttcaat gtgttgtagc agaataggca acatcaccac gccgacaaac
     1561 agcgagaaga gaatgacgcc agccgccagg aacaccagct catagcgcgc cgggaagacg
     1621 ttaccatccg gcaagagcag cgggattgag agcacaccgg ccagagtgat cgccccacgc
     1681 accccagcga aagacgcgat caggatttct cgtgtggtcc acgaaccaaa ctccatcggc
     1741 ttcttcttca ggaagcggtt gctgaacttt ttcatcgtcc acagccagcc gaaacggacc
     1801 agcatcagcg ccgcatatat cagaataata tcggtaaaca gcatccagat ttcgacgtta
     1861 gggtcgattt ctgccgccat cagcgacgtc tccagaatac ccggcagttg cagacctaac
     1921 agcaggaaca ccatgccgtt aaagacaaat tccagcatcg cccaggtgct gtttgcgcgc
     1981 aggcgcattg ccagtggcgc acggcgcatc acaccggagc gggtgatggt catcccggcg
     2041 gcaaccgcag cgaggatgcc cgaaacgcca atatgttcgg caatcagata agaagcgaat
     2101 ggcagcaaga acagcagaac gatctgcgtc gcgggttcat caccgcccca gcggctgagg
     2161 aatcgcagcg agcgaccata cagccagctc accacaaaac cggcaagaat accgccaatg
     2221 gctactttca taaattcgac cgtcgcaccg ccgacagtga agatcatcgt ccccatcgcc
     2281 actgccacgg caaacttcaa cgacaccagg ccggaagcgt cattcatcaa cgcttcgccc
     2341 tgcaaaatgc ccatgatttt tttcgggatg cgcccttcgc caacaatccc ggagagcgcc
     2401 acagcatcgg tcggagaaag taccgccgcc agcgcaaagg cggggatcag cggaatgccc
     2461 ggcaccaccc agtaaataag gaagccaatg ccgaccacgg tgaccaccac cagcgccagc
     2521 gcgaggccga aaatctctcg accatgttcg agaaattcac gggtcggcgt tttccagcca
     2581 tcagcgaaca gcaacggcgg gataaacaag actaaaaaga gttcaggatc aaactccaca
     2641 tgcaaaccaa acgtcggcca cgccagtagc gcaccgatgg cgatttgcat aagcggaagc
     2701 gggatctgaa agggcatgac acgagtgacc accccggaca gcgagaccac gagggtcatt
     2761 atcagtatgg tgaagaagat ttccatgcgt tccctgtttg cgatgttttt tatgatctga
     2821 gaaagacgtt atgcctgaat tctcattttt tcagaatatc gcaaaagctc acacaatgtg
     2881 cggagaaaat aaaaacgggc agccattgcc acccgttttc agaatccgat gaaagattag
     2941 atagcccagc cacccgcata gaaggtcacc agcgccacgg cgataacgac cgtaccgatg
     3001 ttcaacttgc gccattcacc ggaaaccaga cgaccaatca ccagagtcgc gaagccgatc
     3061 atgatgcctg ttacgatgtt acaggtcagc acgatgaata ccgccgtaac cagacccgcc
     3121 atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt tgctcagcat cagcaggcca
     3181 acgtacatca gcgccggagc cgttgcatac cccggaacga ggtaagaaag cggagagagg
     3241 aacagaatca gcaggaacag cacgccaacg gtgatagccg tcaacccggt tttaccgccc
     3301 gccgccgtac ccgccgcaga ctcgatgtat accgctgccg gagctgcacc caccaggcca
     3361 gagaaaacgc tgctcatgga gtcagtggtc agtgctttcc cgccgtcgat gatctgccca
     3421 tctttatcca gcaggttcgc ctggccggcg acagcgcgga tagttccggt ggcatcaaat
     3481 actgccgtca tcaccagcgc cagaacgctt ggcaggacta caggattcag cgcgcccata
     3541 atgtccaggc tgccaatcag tgaattgccg ttttcatcgc tcaatgaagg catggcgaaa
     3601 acgccggaga aatggacgtt aggatcgaag atcaaaccga caattgagat accgataatg
     3661 gtcagcagaa tgccaccagg gactttcagt ttttccaagc cgatgatcac cgccagacct
     3721 accagtgaca taatcaccgg gaaggtcgcg aaatcaccca gcgcaacggg cagaccatca
     3781 agcgggtttt taatcaccag accgacacca ttagcggcaa tgagcagcag gaacagaccg
     3841 ataccgatcc ccgtgccgtg cgccacaccg tgcggcaagt tgcgcaaaat ccagctacgg
     3901 atacccgtgg cagaaattac cgtaaacagc acacccatca ggaacacggc acccagcgcg
     3961 acaggtacgc taatatgttg ccccagcacc aggctgaatg cggtaaacgc cgtcagggag
     4021 atggcgcaac caatcgccaa cggcagatta gcccacagac ccatcacgat agaaccgagt
     4081 ccggcaacca gacaggttgc aacgaaaact gccgcaggcg ggaagcctgc tttacccaac
     4141 atacctggaa cgacgatgac cgagtagacc atcgccagaa acgttgttaa cccggcaacc
     4201 acttcctgac ggacagtgct tccacgttgt gaaattttaa accaggcgtc gagtgaaccg
     4261 ccggtacgcg ctgatggcgt agacataaga aaacatcccc tgagagttta attttcgtca
     4321 agatgcgtgg cggacaatcc cctgccagcg aaacgttatc ttcctgctcc aggtttgcgg
     4381 caatttggtg cgtcgcaaaa aagcaaacgt ttagctccgc gcatacaaaa cgggtggtaa
     4441 aaaaaagcaa acgattatcc ggcgatcata gcggcttttt caaccgaagt ttgctgcttt
     4501 tttctctttt attgtcttga cgatgaaaaa ccaggcaatt gatgcgcaaa cgttatcgtc
     4561 gatgtgcaag attgcagcgt tatcgagccg tataccgttc tggattgcga taaaaaatcg
     4621 tctgagatga caaaaatatt gcggctaaaa agtatacttt tacactggcg aagaaacgtc
     4681 ggtgacatac aattaaagca tcaacaccaa ccggaacctc caccacgtgc tcgaatgagg
     4741 tgtgttgacg tcgggggaaa ccctcctgtg ttccagcggg atagagagaa agacaaagac
     4801 cggaaaacaa actaaagcgc ccttgtggcg ctttagtttt gtgtttgcgg ataattccaa
     4861 aagagctcga tcagtcccct cgccccttcg gggagagggt tagggttagg ggaacaggca
     4921 gcaccagtgc aaatatttac cctcaccccg gccccatagt taacgatgag tcagcttgtc
     4981 cttgtggcgc tttagttttg ttcatcttcc agcaagcgtg cgccggtacc ttcttctcct
     5041 aagcggtcgc ccgggttacg caacgggcaa tcactgcgcg aaaggcagcc acaaccaata
     5101 catccgtcca gttcgtcacg cagcgccact aaggtatgaa tgcgccgatc caactcttct
     5161 cgccattggg acgaaagctg tttccactct ttcgcactta acgtatgccc ttctggcaac
     5221 acgccaaacg cttcaccaat ggtcgccagc ggaatgccaa tacgctgagc gattttgata
     5281 attgcgacat atcgcaacac atcacgttta tatcgccgct gattgccgct gttacggata
     5341 ctagtaatca accctttgct ttcatagaaa tgcagcgccg ataccgccac accgctgcgt
     5401 ttcgccactt cgccaggggt tagcagcgct ttaatgcggg gtaatttctt ttccataaat
     5461 cgctttacct caagttaact tgaggaatta tactccccaa cagatgaatt aacgaactga
     5521 acactgaaaa gaggcagatt tatgtcccat cagaaaatta ttcaggatct tatcgcatgg
     5581 attgacgtgc atattgacca gccgcttaac attgatgtcg tcgcaaaaaa atcaggctat
     5641 tcaaagtggt acttgcaacg gatgttccgc acggtgacgc atcagacgct tggcgattac
     5701 attcgccaac gccgcctgtt actggccgcc gttgagttgc gcaccaccga gcgtccgatt
     5761 tttgatatcg caatggacct gggttatgtc tcgcagcaga ccttctcccg cgttttccgt
     5821 cggcagtttg atcgcactcc cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc
     5881 gttaactccc gcgcgggtaa ttgctccatc cattgcataa acacctgcgg cggcatcgcc
     5941 ttcgcaaaaa accatccctg gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc
     6001 tcctcagttt cgacgccttc agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg
     6061 atgtgttccg caatcaaatg actggttttg tgggtggtca gcgtttcaac aaacgattta
     6121 tcgattttca aaatatcgac attcaatgat ttaaggttat gcaagttaga gtagccaata
     6181 ccaaaatcat caattgccac ttcgtaacca gcctggcgga aagccagaat taatcggcgt
     6241 cattttttcg acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg
     6301 caccgcgtat tgctctgttt tctgattgat tcgcgctatc aaccgtgacg tatgaaaatc
     6361 ggaggccgac aggttaatag aaacatagcg atcggcatgt gttgccaggt acgcgcccag
     6421 atcgcggaag acattatcaa taacataatc agttatctgt tctatcatcc cctccttttc
     6481 tgccagcgga ataaactctg ccggattcat tatttgcccc tgctcaccag gccaacgtaa
     6541 caacgcttca gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag
     6601 acaaagttga tgtttttcga gggcgcgttg caatttacgt ttgggagata aatagttttg
     6661 tcgaatacgt agccagagta atagcagaac aagactcccc aaaataccgg cgggcaacgc
     6721 aaaaataaga tgattataaa aatgggttat aagacgttga tatgaagtcg caacaatggc
     6781 tgcaattggg cgttttgtcg aataaattgt cgcatataaa tagccatttc tttgtacggt
     6841 taaatccttc aaatgaatca gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga
     6901 gaaaaaggtt ttcgtcaccg tatcatacac accccattgc aatgtcgggt catcagacat
     6961 cacttcactc cagaagagag ggttgataac cgccacataa tttccccgct gcatataggt
     7021 cattttatag ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc
     7081 acgcttataa tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca
     7141 taaaaaatgg ttatcacgaa ttcattaata taaagatagc cacgaataat attcaacatt
     7201 cgcttttgat gggctggagt gcaaacttgc ccctgatagc gttcagctgc atcgctcact
     7261 aaatctgcct ggcgaatgac cagctcggat ttatccagtg ccagttgagc aaaggttcgg
     7321 agctgattat tcacttctga ttttgcccaa aggaaggcaa tccatagcga aagaatgatg
     7381 ggaaagagaa ctaaaaagat aatgcccggc aacgccagta attggtgtcg tgcacgatga
     7441 ctcattgcta tcaatttcct gcctgtttat acccagccat tgcaaggtga aaccctggct
     7501 accgacgtct ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga
     7561 taccttttta atcgtcacct ggatgaaaat aattcattga tattcaaaat taattttcta
     7621 caggaataca gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataattt
     7681 gtacagcaat aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagttc
     7741 acaaaattaa tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc
     7801 cagagtgtac cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca
     7861 gaggaattgt ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcaacttct
     7921 tagtgccgta atgatgtttc tggccagcac actcaacatc cgttttcgtc ggtctgatta
     7981 tgtcgggctt gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt ggttcgcaat
     8041 ggggttgctt ggcatcacaa tggcggacat caccgctatc tggcacaaca tcgagtcggt
     8101 gatgatagaa gagatgaatc agacaccgcc acaatggcca atgattttga cctgatacgt
     8161 agaagc
//
LOCUS       DAEBXD010000143         7916 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEBXD010000143 DAEBXD010000000
VERSION     DAEBXD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.143"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            237..740
                     /gene="dps"
                     /locus_tag="KJL94_003616"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="KJL94_003616"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBD4411815.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="KJL94_003617"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="KJL94_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBD4411816.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="KJL94_003618"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="KJL94_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBD4411817.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="KJL94_003619"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="KJL94_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBD4411818.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="KJL94_003620"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="KJL94_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="KJL94_003621"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="KJL94_003621"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBD4411819.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="KJL94_003622"
     CDS             7091..7351
                     /locus_tag="KJL94_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4411820.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="KJL94_003623"
     CDS             7616..>7916
                     /locus_tag="KJL94_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD4411821.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2025 c         1978 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DAEBXD010000144         7864 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEBXD010000144 DAEBXD010000000
VERSION     DAEBXD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.144"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            52..585
                     /gene="ymdB"
                     /locus_tag="KJL94_003624"
     CDS             52..585
                     /gene="ymdB"
                     /locus_tag="KJL94_003624"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBD4411822.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            527..2008
                     /gene="clsC"
                     /locus_tag="KJL94_003625"
     CDS             527..2008
                     /gene="clsC"
                     /locus_tag="KJL94_003625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBD4411823.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2016..3173)
                     /gene="mdoC"
                     /locus_tag="KJL94_003626"
     CDS             complement(2016..3173)
                     /gene="mdoC"
                     /locus_tag="KJL94_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBD4411824.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3567..5102
                     /gene="mdoG"
                     /locus_tag="KJL94_003627"
     CDS             3567..5102
                     /gene="mdoG"
                     /locus_tag="KJL94_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBD4411825.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5095..7638
                     /gene="mdoH"
                     /locus_tag="KJL94_003628"
     CDS             5095..7638
                     /gene="mdoH"
                     /locus_tag="KJL94_003628"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBD4411826.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1940 a         1887 c         2078 g         1959 t
ORIGIN      
        1 accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt aaggaggcgt tatgaaaacg
       61 cgtattcatg ttgtgcaggg tgatattacc aaactggccg ttgatgtgat tgtgaatgcg
      121 gctaatccgt cattaatggg aggcggcggc gtcgatgggg ccattcatcg cgcagcgggt
      181 ccggccctgc tggatgcctg tttaaaagtc aggcaacagc agggcgattg ccctacgggg
      241 catgccgtta ttacgcttgc aggcgatctt cccgctaaag ccgtagtaca caccgtcggg
      301 ccagtctggc gtggtgggga acaaaacgaa gaccagcttt tgcaggatgc ctatctgaat
      361 agcctacgac tggtggcggc aaacagctat acgtcagtgg cttttcctgc aatcagtact
      421 ggggtttata gttaccctcg tgcggcagcg gctgaaatcg cagttaaaac cgtttcagaa
      481 tttattaccc gtcacgcttt acccgaacag gtatactttg tctgttatga tgaagaaaac
      541 gcccacctct acgaaagact ccttacccaa caaggagatg aatgatttgc cccggctggc
      601 gagcgcggtg ctgccactgt gttcgcaaca tcccggtcag tgtggtcttt tccctctgga
      661 gaaaagtctg gatgcgtttg ccgcccggta tcgtctagcc gaaatggcag agcatacgct
      721 ggatgttcag tattacatct ggcaggacga tatgtcgggg cggttactgt tttccgccct
      781 gttagccgca gcaaagcgtg gcgttcgcgt ccgtttgttg ctggacgaca acaatacgcc
      841 cggacttgac gacattttgc gcttgcttga cagtcatcca cgcattgaag tccggctttt
      901 taatcctttc tcgtttcgct tgctacgtcc gcttggatat atcaccgact tttcccgtct
      961 caatcgccgg atgcacaata aaagtttcac tgtcgatggc gtggttaccc tagtgggagg
     1021 acgaaatatt ggtgatgcct attttggtgc aggggaagag ccactttttt cggatttaga
     1081 tgtcatggca ataggccccg tggtagagga tgttgccgat gatttcgccc gctactggta
     1141 ttgcaaatcg gtttcaccct tacagcaggt gctggatgtc ccggaaggtg aaatggcgga
     1201 tcgcatcgag ttacccgcct cctggcataa cgatgccatg acgcatcgtt atttacgcaa
     1261 aatggaatcc agtccattta taaatcatct ggttgatgga acgttgccgc ttatctgggc
     1321 gaagacacgt ttattaagtg atgatccggc gaaaggggag ggcaaggcaa aacggcattc
     1381 actgttaccg cagcgcctgt tcgatatcat gggctcaccc agtgaacgca tcgatattat
     1441 ctcttcctat tttgtaccga cacgcgcagg tgtggcgcaa ctcttacgga tggtgagaaa
     1501 aggggtaaag attgcgatcc taaccaattc tcttgccgct aacgatgttg ctgtcgtcca
     1561 tgccggatac gcgcgctggc gcaaaaaatt gctccgctat ggcgtggaat tatatgaact
     1621 caagccgacg cgtgaacaaa gcagtacgtt acacgatcgc ggcataaccg gtaattccgg
     1681 tgccagcctg catgctaaaa cttttagcat cgatggtaaa acggtgttta tcggctcttt
     1741 caatttcgat ccgcgttcaa cattgctcaa tactgaaatg ggcttcgtga tagagagcga
     1801 aacgctggca cagttaattg ataaacgctt tattcagagc cagtacgatg cggcctggca
     1861 gctccgtctg gacaggtggg gacggatcaa ctgggttgat cgtcatgcaa agaaagaggt
     1921 tattctcaaa aaagaacccg ccaccagttt ctggaagcgg gttatggtca gactggcgtc
     1981 gatattgccc gtggaatggt tattgtaaat gtggcttaac gggctggtgc tttatcgttc
     2041 tcacgcttaa caaccggttt accagagaac aaaaacttca gtaacgggat gcgtaaatga
     2101 atttcataca gaattatcgc aatccctact acgaatatca ggccacagag aaaaccaagc
     2161 cagttggagg tgatgtgcgg tgtaatgtat gcgccgaaaa acagcgttaa cgggtggtga
     2221 accagataga taaacagcga tgcgttaaca aaataagtca cccgcgctga ctggaagtta
     2281 agcaaacggt ggccaaagga gaagaccaca ttcaccatcc acaggccgag gaccatggtg
     2341 atcaccgact cggtttcgta catccaggca tcgccactgc catagcgctg gttgagtaaa
     2401 taagcgacaa acgccaatgc tgcagcgagg gtacagccac gagacggcgt ggtaaacaag
     2461 gctttaagat gagggaaaat gaaagccagt gcgccgagga taaagaacgg taaataaaat
     2521 agcgtttgca tgacaataaa attgaacatg ccattactca gaatgggtgg atacacaata
     2581 aaaatcgttc ttcttattac cgcatagccg atgccgaggc ataaaaaaat caccgatagt
     2641 tttaccatcg agaatttttt attcgtttta tcagaatttt ctaaattatt tctgatgcgc
     2701 ttaaatatcc atacacacag cgtcgtcatg actactaaca ccagtaaaaa ccacaggtgt
     2761 gatattaatt cccaggccaa cgtattatat ttgtcataca atgatagccc aggccaactt
     2821 tccgctttcc ctttgacata ttgcagcata ataaattgcg gtaatgtcag tagggggatg
     2881 gctgttaaca tcgggatacc tacacgttcg acacgtactt tccaccattt tttcaaggga
     2941 tagcgtaaaa aaagcatgta ggaaaagtat ccggatataa cgaaaaatac ctgcatgcgg
     3001 aacgagtgga tgaagtcatt aaaaagggtt agccacaatg acggttcggc gctattcaca
     3061 tgccatgtat ggctcgaata gattaaagaa atatgaaaag ggatccctaa caacatcagc
     3121 caggcgcgga tggagtcgag gaaatattca cgttgcgcgg gtactggatt catatatggt
     3181 taactaatct cggatttttc gtcttatccc tgtcgggtta tgcctttagg cttgttgcca
     3241 tagcgacacc gacctgaccg cgccaggcgc aggcttcaag gtttttatgc atagcatcat
     3301 cgctaccact aaccagaatg gaagcgtctg taagacggtt gataaataaa tttgctggca
     3361 aaccctacac gaagtcgatg cttctgtctt taggagaagc acggaaagtg aaaacggttg
     3421 caatcaggtg cttaatccat gagccagcgt gctgaacgat accgggattc agttgtcgga
     3481 atggctggtt atccattaaa atagatcgga tcgatataag cacacaaagg gggaagtgct
     3541 tactaattat gaaacataaa ctacaaatga tgaaaatgcg ttggttgagt gctgcagtaa
     3601 tgttaaccct gtatacatct tcaagctggg ctttcagtat tgatgatgtc gcaaagcaag
     3661 ctcaatcctt agccgggaaa ggctatgagg cgcctaaaag caacttgccc tccgttttcc
     3721 gcgacatgaa atacgcggac tatcagcaga tccagtttaa tcatgacaaa gcgtactgga
     3781 acaatctgaa gaccccattc aaactcgagt tctaccatca gggtatgtac ttcgataccc
     3841 cggtcaaaat aaatgaagtg actgccaccg cagtcaaacg aatcaaatac agcccggatt
     3901 atttcacttt cggcgatgtt cagcatgaca aagacacggt aaaagacctt ggttttgccg
     3961 gttttaaagt gctttacccg atcaacagca aagataaaaa cgatgaaatc gtcagcatgc
     4021 tcggggccag ctatttccgc gtgattggtg caggtcaggt ttatggcctt tctgcccgcg
     4081 gcctagcaat tgataccgct ttgccatcgg gtgaagaatt tccacgcttc aaagagttct
     4141 ggatcgagcg tccaaaaccg actgataaac gtttaaccat ttatgcattg cttgactcgc
     4201 cgcgtgcgac aggtgcttac aaattcgtgg ttatgccagg gcgtgacacg gttgtggatg
     4261 tgcagtcgaa aatctatctg cgcgataaag tcggcaaact gggggttgca ccgttaacca
     4321 gtatgttcct gtttgggccg aaccaaccgt cgcctgcaaa taactatcgt ccggagttgc
     4381 acgactctaa cggtctgtct atccatgctg gtaatggcga atggatctgg cgtccgttga
     4441 ataacccgaa acatttagcg gtcagcagct tctccatgga aaacccgcga ggctttggtc
     4501 tgttgcaacg aggtcgtgat ttctcccgct ttgaagatct cgatgatcgt tacgatctcc
     4561 gtccaagcgc atgggtgact ccgaaagggg agtggggcaa aggcagcgtt gagctggtgg
     4621 aaattccaac caacgatgaa accaacgata acatcgtcgc ttactggacg ccggatcagc
     4681 tgccggagcc gggtaaagag atgaacttta aatacaccat caccttcagc cgtgatgaag
     4741 acaaactgca tgcgccagat aacgcatggg tgcaacaaac gcgtcgttca acgggggatg
     4801 tgaagcagtc gaacctgatt cgccagcctg acggtactat cgcctttgtg gtcgatttta
     4861 ccggcgcaga gatgaaaaaa ctgccagagg ataccccggt cacagcgcaa accagcattg
     4921 gtgataatgg tgagatagtt gaaagcacgg tgcgctataa cccggttacc aaaggctggc
     4981 gtctggtgat gcgtgtgaaa gtgaaagatg ccaagaaaat cactgaaatg cgtgctgcgc
     5041 tggtgaatgc cgatcagacg ttgagtgaaa cctggagcta ccagttacct gccaatgaat
     5101 aagacaactg agtacattga cgcaatgccc atcgccgcaa gcgagaaagc agcattgccg
     5161 aagactgata tccgcgccgt tcatcaggcg ctggatgccg aacaccgcac ctgggcgcgg
     5221 gaggatgatt ccccgcaagg ctcggtaaag gcgcgtctgg aacaagcctg gccagattca
     5281 cttgctgatg gacagttaat taaagacgac gaagggcgcg atcagcttaa ggcgatgcca
     5341 gaagcaaaac gctcctcgat gtttcccgac ccgtggcgta ccaacccggt aggccgtttc
     5401 tgggatcgcc tgcgtggacg cgatgtcacg ccgcgctatc tggctcgttt gaccaaagaa
     5461 gagcaggaga gcgagcaaaa gtggcgtacc gttggtacca tccgccgtta cattctgttg
     5521 atcctgacgc tcgcgcaaac tgttgtcgcg acctggtata tgaagaccat tcttccttat
     5581 cagggttggg cgctgattaa tcctatggat atggttggtc aggatttgtg ggtttccttt
     5641 atgcagcttc tgccttatat gctgcaaacc ggtatcctga tcctttttgc ggtactgttc
     5701 tgttgggtgt ccgccggatt ctggacggcg ttgatgggct tcctgcaact gcttattggt
     5761 cgcgataaat acagtatatc tgcgtcaaca gttggcgatg aaccattaaa cccggagcat
     5821 cgcacggcgt tgatcatgcc tatctgtaac gaagacgtga accgtgtttt tgctggcttg
     5881 cgtgcaacgt gggaatcagt aaaagccacc gggaatgcca aacactttga tgtctacatt
     5941 ctgagtgaca gttataaccc ggatatctgc gtcgcagagc aaaaagcctg gatggagctt
     6001 atcgctgaag tcggtggcga aggtcagatt ttctatcgcc gccgccgtcg ccgcgtgaag
     6061 cgtaaaagcg gtaatatcga tgacttctgc cgtcgctggg gcagccagta cagctacatg
     6121 gtggtgctgg atgctgactc ggtaatgacc ggtgattgtt tatgcggcct ggtgcgcttg
     6181 atggaagcca acccgaacgc cgggatcatt cagtcgtcgc cgaaagcgtc cggtatggat
     6241 acgctgtatg cgcgctgtca gcagttcgcg acccgcgtgt atgggccact gtttacagcc
     6301 ggtttgcact tctggcaact tggcgagtcg cactactggg gacataacgc gattatccgc
     6361 gtgaaaccat ttatcgagca ctgcgcactg gctccgctgc cgggcgaagg ttcctttgcc
     6421 ggttcaatcc tgtcacatga cttcgtagaa gcggcgttga tgcgccgtgc aggttggggg
     6481 gtctggattg cttacgatct cccgggttct tatgaagaat tgccgcctaa cttgcttgat
     6541 gagctaaaac gtgaccgccg ctggtgccac ggtaacctga tgaacttccg tctgttcctg
     6601 gttaagggta tgcacccggt tcaccgtgcg gtgttcctga cgggcgtgat gtcttatctc
     6661 tccgctccgc tgtggtttat gttcctcgcg ctctctactg cattgcaggt agtgcatgcg
     6721 ttgaccgaac cgcaatactt cctgcaacca cggcagttgt tcccggtgtg gccgcagtgg
     6781 cgtcctgagc tggcgattgc actttttgct tcgaccatgg tgctgttgtt cctgccgaag
     6841 ttattgagca ttttgcttat ctggtgcaaa ggaacgaaag aatacggcgg cttctggcgc
     6901 gttacattat cgttgctgct ggaagtgctg ttttccgtgc tgctggctcc ggtacgcatg
     6961 ctgttccata cggtcttcgt tgtcagcgcg ttccttggct gggaagtggt gtggaattca
     7021 ccgcagcgtg atgatgactc cacttcatgg ggcgaagcgt tcaaacgcca cggctcacag
     7081 ctgctgttgg ggttagtgtg ggctgttggg atggcgtggc tggatctgcg tttcctgttc
     7141 tggctggcac cgattgtctt ctcgttgatc ctgtcaccgt ttgtttcggt gatttccagc
     7201 cgtgccaccg ttggtctgcg caccaaacgc tggaaactgt tcctgatccc ggaagagtat
     7261 tcaccgccgc aggtgctggt tgataccgat cggttccttg agatgaatcg ccaacgctcc
     7321 cttgacgatg gctttatgca cgcagtgttt aacccgtcat ttaacgctct ggcaaccgca
     7381 atggcgacag cgcgtcaccg cgccagtaag gtgctggaaa tcgcccgtga ccgccacgtt
     7441 gaacaggcgc ttaacgagac gccagagaag ctgaatcgcg atcgtcgcct ggtgctgcta
     7501 agcgatccgg tgacgatggc ccgtctgcat ttccgcgtct ggaattcccc tgaaagatat
     7561 tcttcatggg tgagttatta cgaagggata aagctcaatc cactggcatt gcgtaaaccg
     7621 gatgcggctt cgcaataaaa acgtagttgc ctgatgcgct acgcttatca ggcctacatc
     7681 gttcctgcaa tttattgatt ttgcaagatt ttgtaggtcg gataaggcgt tcacgccaca
     7741 tccggcaaaa acaacgagcc aataaaaata ccggcgttat gccgtaatgc cagtcagtta
     7801 agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac
     7861 tctc
//
LOCUS       DAEBXD010000145         7844 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEBXD010000145 DAEBXD010000000
VERSION     DAEBXD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.145"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="KJL94_003629"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="KJL94_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KJL94_003630"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KJL94_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBD4411827.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KJL94_003631"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KJL94_003631"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBD4411828.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KJL94_003632"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KJL94_003632"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBD4411829.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="KJL94_003633"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="KJL94_003633"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBD4411830.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1783 a         2040 c         2113 g         1908 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga aagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cggg
//
LOCUS       DAEBXD010000146         7768 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEBXD010000146 DAEBXD010000000
VERSION     DAEBXD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.146"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..305
                     /locus_tag="KJL94_003634"
     CDS             <1..305
                     /locus_tag="KJL94_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBD4411831.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2205
                     /locus_tag="KJL94_003635"
     CDS             277..2205
                     /locus_tag="KJL94_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBD4411832.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2189..2392
                     /locus_tag="KJL94_003636"
     CDS             2189..2392
                     /locus_tag="KJL94_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4411833.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="KJL94_003637"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="KJL94_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="KJL94_003638"
     CDS             3972..5477
                     /locus_tag="KJL94_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBD4411834.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="KJL94_003639"
     CDS             5514..5861
                     /locus_tag="KJL94_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBD4411835.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="KJL94_003640"
     CDS             5919..6947
                     /locus_tag="KJL94_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBD4411836.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="KJL94_003641"
     CDS             6999..7385
                     /locus_tag="KJL94_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD4411837.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..>7768
                     /locus_tag="KJL94_003642"
     CDS             7397..>7768
                     /locus_tag="KJL94_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4411838.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEG"
BASE COUNT         1793 a         1814 c         2411 g         1750 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgcc gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgcgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac ctgggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtgttttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgctggcggc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gccaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgtgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tggctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagtcctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca ggcagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggcagcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacgacccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaaggg
//
LOCUS       DAEBXD010000147         7719 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEBXD010000147 DAEBXD010000000
VERSION     DAEBXD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.147"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..830)
                     /locus_tag="KJL94_003643"
     CDS             complement(<1..830)
                     /locus_tag="KJL94_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBD4411839.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="KJL94_003644"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="KJL94_003644"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBD4411840.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="KJL94_003645"
     CDS             complement(2479..2706)
                     /locus_tag="KJL94_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBD4411841.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KJL94_003646"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KJL94_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBD4411842.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KJL94_003647"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KJL94_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBD4411843.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KJL94_003648"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KJL94_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBD4411844.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="KJL94_003649"
     CDS             complement(5528..6511)
                     /locus_tag="KJL94_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4411845.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="KJL94_003650"
     CDS             complement(6557..7381)
                     /locus_tag="KJL94_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4411846.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KJL94_003651"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KJL94_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBD4411847.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcact
//
LOCUS       DAEBXD010000148         7592 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEBXD010000148 DAEBXD010000000
VERSION     DAEBXD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.148"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..695
                     /locus_tag="KJL94_003652"
     CDS             <1..695
                     /locus_tag="KJL94_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBD4411848.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="KJL94_003653"
     CDS             complement(1034..1804)
                     /locus_tag="KJL94_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411849.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="KJL94_003654"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="KJL94_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="KJL94_003655"
     CDS             complement(2295..2516)
                     /locus_tag="KJL94_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411850.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="KJL94_003656"
     CDS             complement(2517..3200)
                     /locus_tag="KJL94_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD4411851.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="KJL94_003657"
     CDS             complement(3264..3575)
                     /locus_tag="KJL94_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411852.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="KJL94_003658"
     CDS             complement(3572..4474)
                     /locus_tag="KJL94_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBD4411853.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQSCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="KJL94_003659"
     CDS             4914..5873
                     /locus_tag="KJL94_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBD4411854.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="KJL94_003660"
     CDS             5873..6268
                     /locus_tag="KJL94_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBD4411855.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="KJL94_003661"
     CDS             6451..6831
                     /locus_tag="KJL94_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBD4411856.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQHITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6877..7592
                     /locus_tag="KJL94_003662"
                     /pseudo
     CDS             6877..7592
                     /locus_tag="KJL94_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2063 a         1958 c         1858 g         1713 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagct ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aatatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagcatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     6961 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccagt
     7021 gcaccatggc gcgatttacc cgaacgatat ggggcatgga aaactgttta taaccgcttt
     7081 aaccgatggt caaagtcagg cgtgattaat attattttca acaggttgct ttcgctactt
     7141 gatgcaaacg gctttattga ctggtctgcc accgcgctgg atggcagtaa tatccgggcg
     7201 ctgaaatgtg ccgccggtgc tcaaaaaaac atcccgatat cgacggagat aatgggccgg
     7261 gtcgctctcg cggcggtttt ggcaccaaaa tccatctggc aacagacgga agtggcctcc
     7321 cgttaaatat cgtgctaagc cccggacaag ctcatgaaag ccagttcgcg caacgacttc
     7381 tggacgggat tggcgttcag cgtcagaacg gcagcatgaa acgccgtgga catgcggtac
     7441 tggctgataa agcgtattca gggcgcgcat tgcgcaacga gctgaaaaat aacggtataa
     7501 aggcagtaat cccccgaaag tcaaatgaga aaatggcatc ggatggacgt gcacagcttg
     7561 atcgtgatgc gtactgcaac cgtaatgtcg tt
//
LOCUS       DAEBXD010000149         7581 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEBXD010000149 DAEBXD010000000
VERSION     DAEBXD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.149"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(106..1733)
                     /gene="yeeO"
                     /locus_tag="KJL94_003663"
                     /pseudo
     CDS             complement(106..1733)
                     /gene="yeeO"
                     /locus_tag="KJL94_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1738..1813
                     /locus_tag="KJL94_003664"
     tRNA            1738..1813
                     /locus_tag="KJL94_003664"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1771..1773,aa:Asn,seq:gtt)
     gene            complement(1851..2801)
                     /gene="cbl"
                     /locus_tag="KJL94_003665"
     CDS             complement(1851..2801)
                     /gene="cbl"
                     /locus_tag="KJL94_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBD4411857.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2903..3820)
                     /gene="nac"
                     /locus_tag="KJL94_003666"
     CDS             complement(2903..3820)
                     /gene="nac"
                     /locus_tag="KJL94_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBD4411858.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4148..4223
                     /locus_tag="KJL94_003667"
     tRNA            4148..4223
                     /locus_tag="KJL94_003667"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4181..4183,aa:Asn,seq:gtt)
     gene            complement(4279..5214)
                     /gene="ldtA"
                     /locus_tag="KJL94_003668"
     CDS             complement(4279..5214)
                     /gene="ldtA"
                     /locus_tag="KJL94_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD4411859.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5276..6355)
                     /gene="cobT"
                     /locus_tag="KJL94_003669"
     CDS             complement(5276..6355)
                     /gene="cobT"
                     /locus_tag="KJL94_003669"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBD4411860.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6367..7110)
                     /gene="cobS"
                     /locus_tag="KJL94_003670"
     CDS             complement(6367..7110)
                     /gene="cobS"
                     /locus_tag="KJL94_003670"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBD4411861.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7107..>7581)
                     /gene="cobU"
                     /locus_tag="KJL94_003671"
     CDS             complement(7107..>7581)
                     /gene="cobU"
                     /locus_tag="KJL94_003671"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBD4411862.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2091 a         2054 c         1644 g         1792 t
ORIGIN      
        1 gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt
       61 ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacaac
      121 tggctttttt tcacactttt gcggctcgga tcgagggtat ttccacagcc aacgtccagt
      181 aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca
      241 gacaccaacc acaccccagc caagcatgat tccctgcaca taaccgacta caacccggca
      301 accccacatg ctcaacatag aaacccacat ggcgtaacgg gcatcacgag cacccttaaa
      361 tccagcgggt agcacccatg aggcggacca aataggcata aataaagcat ttagctaaat
      421 cagaatcaca acgacgtgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc
      481 ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggaca gccagaacac
      541 atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat
      601 gatcgtagaa gcagaaccga gcgcacttcc gggtaagttg ataagagccg caattgagaa
      661 cgcgataaaa tttccggcaa taacgctggt ccccatcccg gcaacgaaca tttgggttaa
      721 taaccgacca ctggtaaata acactgattc gacactcgct ggaataccaa tccccatgac
      781 ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc
      841 aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat
      901 ggttaaaccc aacccagccc cgaccaggag aaaaggccgt aaatcaatat gccgctaata
      961 ataatattaa gaatattcag gctaccgtta atcagtagcg gtattttcgt attccctgca
     1021 ccacgaagtg ccccgctacc aataagagtg atggcagcgg ctggataact gagtacggtc
     1081 agctccagat aagtcaacgc cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa
     1141 tcaataattt gttcgccaaa atgatgaatt agcgttgcca acagtacggc aaacaacgtc
     1201 atgatcacca atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga
     1261 ctaaatgcta caacgacagt agtaccaaga tcgatagctg caaaaaaagc cataatgacc
     1321 atattgaagc tgtccgccaa ccccacgccg gccatcgcat cttttcccag ccaactgacc
     1381 agaaaagtgc tcaggccccc catcaacagg acacaggcgt tctccatgaa gataggaaca
     1441 gcaagcgggg ttatctcgcg ctagaacaac actttgtagc tcttgcgttt agagtgccag
     1501 cgagtgccgt gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc
     1561 agaaagtgaa accacatttc aaatgatgag ggagaatcag caaagctgca aagattttcg
     1621 ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttgggaaat
     1681 ttaaaaatcg ggtttgacaa aatatttttc gccgttaaga tgtgcctcaa cagcgattcc
     1741 tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca
     1801 gtcagaggag ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga
     1861 taatcaattt cctcttcact gttttccatc acctggcgct tgatatcctc aactgacagt
     1921 cctgcattac aaaattccag aaagcgccaa acatagttac gctgaagttg tcctcgcttc
     1981 agtcccaacc agacagtatt agcatcgaaa agatgccgcg tatccaggcg gattaaattc
     2041 tcttcctctt gttcgccgct ggattgttcg gcaactaatc cgatcccaag cccaagagca
     2101 acataggttt taatgacatc agaatcctgt gcacttaata caatatctgc cggcaaacct
     2161 ttgcgggtaa atgcgtcatc agtgcgtgag cgccccgtaa ttccctgtcg gtaagtaatt
     2221 aacggccact tcgctattga ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg
     2281 agtggaacaa gcaaactatg gtgccaacaa aaccacggga aggcgacgag ctgcgggtca
     2341 ttactcaaac gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca
     2401 atttcctgtg gcgaaccctg gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa
     2461 gctttaatga cctctggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga
     2521 acaccagacg tatcgttggt aaacaggtct gcaagccgac gaacattact ggcttcattc
     2581 agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt cagtcatccc cagcagtcgc
     2641 ttacctcgtc gaacaaatat ttcgatgcca aattcatcct ccagttcccg aatatgacgg
     2701 ctaacgcctg actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga
     2761 cgtgcagcct cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagttc
     2821 agatatcccg ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc
     2881 ttattccatc ccgatataac gcttagctca ccaattgcca ctgccttttt tccatcactg
     2941 gagaactaat cactgacatt aacaactctt tcactgcctg tgcctgtggt gacaagtttg
     3001 ctctggcggg taaatttaag gacaaagaga gactcattga aggagtggta atgcgtgaca
     3061 tccacccatt tactgcgcca cataacgaac gcgcagccga ttcaggtaat actgcaacgc
     3121 ccatgccgct ggcaatcgct gcggtaagcg tggcaataga ctcaatttca ccaatgactt
     3181 ttgccgtgag ccgccgtagg gaaaaagcct catcaacacg aagtctaaca gcgctgtaat
     3241 cactggggag aaaaaggttc atttgcgcaa tagcattaac atcaacgttt tgccccgggc
     3301 aatcttgagt tcctaccagg aaaagatctt ctttcagcaa agcctgactg gataccccag
     3361 ccacagggga atgctcataa atcaccgcca tatcgagttg gtgatttatc agtttttcgt
     3421 taagcactgc accactattt tcatgaagat agataacgac ctccgggaat tcagcgcgaa
     3481 ccgcctgtaa taagggcatg gtgatggatg acgcagcggt tcctggcgca aagccaatcg
     3541 agacttgccc cgataatgac tgaccaacgt tatgcaccgc cagttgggcc tgctcacact
     3601 gacgtaaaat ggcccgtgca tgggtataga taatttttcc ggcgtctgtt ggcgtaacgc
     3661 cccgttttgt gcgaatcaaa agctgttgat ttaactcacc ttccagtatg gcaacctgct
     3721 ggctgagcgc tggttgtgcg atatgcaata cttcagcagc ctgggtcagg ctaccaatat
     3781 ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg gtttttaaga
     3841 atcggcccaa gtgccgccat tacttacaac cagtatgcaa gatgcctgcc agttctattt
     3901 tggcgttgat gcgcaagcta accaactgtc aaataagaga ttatgataga ttcgtcattg
     3961 gcccttttta tcagcagtcg cattcacctg ccctataaaa ggagggtatg caccacgatg
     4021 gttcattacc caataagatt gaaagctcac cactttgttg aaattggcag caaacaaaca
     4081 aaaaaaatgc atttcaccct ttgacatcac catgcactgc cattaatatg cgccccgttc
     4141 acacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg
     4201 ttcgagtcca gtcagaggag ccaaattcaa aaaagcctgc tttcgagcag gctttttact
     4261 ttctaattac caacgctctt aaaacatctg tcttgaacca gaactaattt gcacaggcat
     4321 tcccgatcga tgttgcaacg cagcatttgc gcgatttaca tcaacttctt gcccgttgat
     4381 aaacgcccgc aaagatgggg ttaccggcaa tggcactttt cggtcagact catattctgc
     4441 acgattgcgc gacagcggct catgaacttc cagccacttt gaaccatccg gttccgttgt
     4501 gtatttcact ggctgatcaa taatttgtac acgagtgcct acaggaacat tatcaaacag
     4561 atatttgata tcgtcattac gcagacgaat acagccctga cttacacgga gcccaatacc
     4621 aaaattggca ttggtaccgt ggatggcata caacctgcca atataaatcg cgtacagccc
     4681 catggggtta tcgggccccg caggaacaaa tgcgggcaaa ctctcccctc gtttcgcata
     4741 ttcgcgccgg gtgttcggcg ttggcgtcca ggttggtgct tcttgtttac gttcaacggt
     4801 agtcacccag ttacgcgggg tttctcgccc agcctggccg ataccaatag gaaagacttc
     4861 cactgtatta ctgtctggcg gatagtaata aagacgcatt tcagcgacgt taaccacaat
     4921 ccctttacga acagtggcgg gcaaaatcag ttgctgcgga atggtgagtt gtgagccagg
     4981 cttcggcaaa aaaacatcag cgcccggatt cgcttccagc atattactta acccttgccc
     5041 gtattgtgcg gcaaaagtct ccagcggctg agtattgtga tcaggaacag ttacagtaag
     5101 cgactgcccc actaaacggc taccctctgg aggtaatgga taagttaccg ccaggctatt
     5161 ttggctggca aaaagcagag caaatgagca aagaatattt acacgacgca tcatgtccct
     5221 ttcctaagtc gcgaatacta tccgataagt atagctttta tcagactttt cgtttttagc
     5281 tgttcaaatc agaagtcgta ttccccggta gaacaatatt actggcagca agttcgccca
     5341 tgttgttgta tatcgcacaa gcagcttcga tgatggacat cgccagagct gcaccactcc
     5401 cctcacctaa acgcatatcc atattgagat aaggctccag ccccaaatgc gagagcgcta
     5461 tacgcgcgcc tttttctgcc gacaagtgag aaggaatgag atacggtttg attgcaggag
     5521 acatctggca ggctgcgagc gcagcagcat aagaaagaaa tccatccagc aacacgggta
     5581 aaccgcagga agcagcacct aacatcactc cagctattcc gaccaaatca aatccaccca
     5641 cttttgccag gacatcaaca ccatcctgag gatttggttg attcaacgta atcgcccgac
     5701 gcacaacatc aattttatta gccagtttat ctgtcggcag gtttgcgcca atcccaacca
     5761 cttcttcagg atcccggcca gtgattgtgc tgactattgc cgctgccggt gtcgtgtttg
     5821 ccatccccag ttcacctaca ccaaacagcg tgacaccgtt tttcgccagc tcccgcgtat
     5881 aacatatgac gtccaaaagc aacttttcag cctgacggcg actcattgcc ggagctgaag
     5941 caatattgcc gctacctcgt gcgacacgca tgttgataag cccggggata ggctcagcag
     6001 tatcaatacc aacatcaatt acgtggacgt tagcgcccgc ttgtgctgcc agcacacaca
     6061 cgccggttgt tccacgggtc atattttcag cctgtatggc tgtcacttct tttggagaaa
     6121 tagcgacccc ttcctcccag acgccgtgat cggcacacat aaccagtacc gcttttttgc
     6181 ccacatgcgg tatgccattc acccccggca ttcctgccag ttgtatggca agcgcctcca
     6241 gctttcctag gctaccaaca ggtttgaata acccgtcaac atgccgttgt gcacgcgaca
     6301 tagcggcaga attgatggct gggatcgtat tcagtaaatc ggcaattatt tgcatgtcac
     6361 gtttctttat agcagtgcca gcaggaagac cagttcacca agctctatgg ctgctcccag
     6421 cgtatcgccc gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga tgaaaatcgc
     6481 caccatcgtc accaccatag cagccacacc atgcataccc agcaataata cggcagcaaa
     6541 aatagcggct aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc caatgaacac
     6601 attgccaagc ccttcttccc gcgcataacg atgacgatac atcaataatg cggcagtccc
     6661 gcggctgacc gcacatgctg ccgccagcga tgcaaggatc ggttcgccac gcagagccaa
     6721 ctcgcttaac accagaatct ttgccagtac cacaaaaatc aatgccagac cgccgtgggt
     6781 gcctaaacgg ctatcacgca tgatttccaa catgcgatcg cgactacgtg cagaaaatac
     6841 gccatcacag gtatcagcga ggccgtccag atggaattcc ccggtcatca gcgccagcac
     6901 aagtacgcta aacagtgccg ccagtggtgc gccacaccat gcctgcagca ccatgaagac
     6961 cagcccgcta atcgcgccaa gcaataatcc aatcaaagga aaagtaataa taccgcgaga
     7021 ataatgctcg aaatccagtc cctgggacca gcgacgcggt acaggcagac gcgtaataaa
     7081 tgagagcatc gcccaaaata atttgctcat ttgattttta ctccaatacc cgaaaccacc
     7141 agccatactt catttgccgc agcggccaac tgctgattta cccgcccggc aatatcacga
     7201 aaatgtcgtg ccagacgact ctccggcaca atccccattc ccacttcgtt agtcactaat
     7261 acaacctttg cggggcaacg ttggcaggca gcaatcaacg actgaatctc agcattaatc
     7321 gcctgttcca tcgcctgata atcccattca tcagggtctt tatcgccgcc ataatcaaac
     7381 aacagattag tcaccattgt ggtaacgcat tccagcaaca cagcctcatt cgggttaatg
     7441 tctgcatgaa ttaattcatc aagatgttgc cagcgctcca ctgtgcgcca gtgctccggg
     7501 cggctttgcc gatgatgttc tatccgtgca gccatctcat catcaaggat ttgcgaggta
     7561 gcgatataca gaacctgtga a
//
LOCUS       DAEBXD010000150         7554 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEBXD010000150 DAEBXD010000000
VERSION     DAEBXD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.150"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="KJL94_003672"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="KJL94_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="KJL94_003673"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="KJL94_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBD4411863.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="KJL94_003674"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="KJL94_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="KJL94_003675"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="KJL94_003675"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBD4411864.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="KJL94_003676"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="KJL94_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBD4411865.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KJL94_003677"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KJL94_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBD4411866.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="KJL94_003678"
     CDS             complement(6510..7202)
                     /locus_tag="KJL94_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBD4411867.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DAEBXD010000151         7460 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEBXD010000151 DAEBXD010000000
VERSION     DAEBXD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.151"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(32..107)
                     /locus_tag="KJL94_003679"
     tRNA            complement(32..107)
                     /locus_tag="KJL94_003679"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Thr,seq:tgt)
     gene            complement(110..186)
                     /locus_tag="KJL94_003680"
     tRNA            complement(110..186)
                     /locus_tag="KJL94_003680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Arg,seq:tct)
     gene            complement(188..264)
                     /locus_tag="KJL94_003681"
     tRNA            complement(188..264)
                     /locus_tag="KJL94_003681"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Ile,seq:cat)
     gene            complement(464..844)
                     /locus_tag="KJL94_003682"
     CDS             complement(464..844)
                     /locus_tag="KJL94_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD4411868.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(837..1037)
                     /locus_tag="KJL94_003683"
     CDS             complement(837..1037)
                     /locus_tag="KJL94_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBD4411869.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(1132..1389)
                     /locus_tag="KJL94_003684"
     CDS             complement(1132..1389)
                     /locus_tag="KJL94_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411870.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1386..2777)
                     /locus_tag="KJL94_003685"
     CDS             complement(1386..2777)
                     /locus_tag="KJL94_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD4411871.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2774..3652)
                     /locus_tag="KJL94_003686"
     CDS             complement(2774..3652)
                     /locus_tag="KJL94_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD4411872.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3663..4571)
                     /locus_tag="KJL94_003687"
     CDS             complement(3663..4571)
                     /locus_tag="KJL94_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD4411873.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4558..4791)
                     /locus_tag="KJL94_003688"
     CDS             complement(4558..4791)
                     /locus_tag="KJL94_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411874.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4788..5804)
                     /locus_tag="KJL94_003689"
                     /pseudo
     CDS             complement(4788..5804)
                     /locus_tag="KJL94_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            5736..5999
                     /locus_tag="KJL94_003690"
     CDS             5736..5999
                     /locus_tag="KJL94_003690"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD4411875.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5989..6297
                     /locus_tag="KJL94_003691"
     CDS             5989..6297
                     /locus_tag="KJL94_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4411876.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6339..6515)
                     /locus_tag="KJL94_003692"
     CDS             complement(6339..6515)
                     /locus_tag="KJL94_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBD4411877.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(6512..6922)
                     /locus_tag="KJL94_003693"
     CDS             complement(6512..6922)
                     /locus_tag="KJL94_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBD4411878.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(6919..7197)
                     /locus_tag="KJL94_003694"
     CDS             complement(6919..7197)
                     /locus_tag="KJL94_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411879.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(7272..>7460)
                     /locus_tag="KJL94_003695"
     CDS             complement(7272..>7460)
                     /locus_tag="KJL94_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411880.1"
                     /translation="ASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIAN
                     MPKSEYDEAWRQENVTEY"
BASE COUNT         1779 a         2018 c         1719 g         1944 t
ORIGIN      
        1 gaggtaatag ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg
       61 acctactgat tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac
      121 cgaggactcg aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag
      181 tggtggttgg tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc
      241 tctgaccaac tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc
      301 aaactcatct gaccaccagc gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc
      361 cgcccggagg cgggtttaag ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt
      421 ttttacgtac gtaaaatatt ttctagaaag tcgcccctta ccatcaggga tgttcaatat
      481 atttgtccat ttctaaccgg acacccaaca tcatcaacat tccttccacc accccctcgg
      541 ctttttgtag cttctttcct atataaccat ctgagcatcc atgcttccgc gccagcgtca
      601 tgaatgtcat cccaaacacg tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc
      661 ggttaaggcg ggccatacac ccacatataa ccatcgcgtc atcgtcacag cattgcgggc
      721 gggattttac ttttgaaggg attaatccct taaaaccggc agcaatggac gaccaggtca
      781 catcctcatg attattagcc acccacgcgc cccagcgctc aagaacctgc tgaatatcac
      841 gcatcactgt ctttacccct gtcccatcca cgatgaacaa tcagaacacc atcaacaatg
      901 gcgtgccctt tgccctcttt atcttcagca tattttctta ccgtggcgcg attacagttc
      961 agcattcgtg aaacttcggt catattacct cgtgtctgga taagcagttc cggtatcgtt
     1021 tgaattttga cgctcatcaa atactctccg gttcggtgat ttttatcccg ggctttccac
     1081 cagggacata atgatcgcgc acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag
     1141 cgcctcctgc accagttttt caaactttcc gactctggtt tccagctctg ccacacagtc
     1201 caccagctca tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc
     1261 gggtaccata tctttacgga tagcatcttt tgttatgccc gttttttcga gttgttcagc
     1321 atgacgcagc atttcctgcg cgtgtttacg caattgttca ggggtaaaag tcattgtctg
     1381 gttgttcaaa agaaacgctc catcttactg ctgtcagttc gtttattact gtatctgcgc
     1441 ggattgccgg gcttcatggg agtggaaagc acccgtgcac tttcctggtc cacaggcaga
     1501 aaatgtccgt tataaaaacg ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg
     1561 atattgattt ctgcgatgcc cctggcctgc gtatccgggt tgtacacttc atccctgtaa
     1621 agcatcagaa tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc
     1681 atgggacgtt tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac
     1741 ggaaaaccac cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg
     1801 tcatgacgcc ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc
     1861 gtttccggat atttaatcag gtggtgtttc gttgttgcgc atatctcatc aatggccagg
     1921 ttcgcctggt ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa
     1981 cgcgtccagt cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct
     2041 ccagccgcag acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga
     2101 agcacaccat gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc
     2161 cccatcgaag gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt
     2221 gcgtccagtt catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg
     2281 cattcaacac gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg
     2341 ccctttcctg tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct
     2401 gcttcacagg tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca
     2461 tcgcgcagtg cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc
     2521 caggttttac gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac
     2581 agcacggggt caatcactcc tgaaacacgg gcctgtctgc agatgcctgt atagatatcc
     2641 tgatacgctc gtacagaaaa aacgtccgct ggcagtgtgg ccagaatatc catcacttca
     2701 ggatctgctc cgcgcagaaa gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc
     2761 catactggag ttgtcatgca gccacacctc tgatacgaga acggtaactg ggccagttaa
     2821 acgacaacca gttgcgtccc ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg
     2881 cccacaactc ttccggtgag aggttactga taagaatggt gggcaagata ctttcgtacc
     2941 tggcgttgat aatttcctgc aaaatagcca tttcagccgc gctgccaaac tgaacgccaa
     3001 cttcgtcgat gatcagcaaa tccattgacg cataacgctc aataacttca tccgctgttt
     3061 tttcgctgtc attccgccag caatttttca cagcacgggt aaggcgcatc acgtcggtga
     3121 tctccacact ggccagatag ttacggatga tgtgttttgc catagccaca gccagatgat
     3181 tttttccggt accacaactg ccggtcataa caagactggt accgttctcc agcatatctg
     3241 gccagttctc cgcatagcgg cgacaggccg caagatttct ggctgcgcca ggattaacct
     3301 ccagataatt atcaaactcg cagtcccgaa aacgcagggc aattccggcg ttatcagtca
     3361 gctcttccgc cttgatggac gacagctcca tggtcaaatc gttggcctca gcgattaagc
     3421 agtcagggca gcatgaaatt tcttctctgt cctcgccatt acgatcgctc cacaccagta
     3481 tatgcgtgtg atattcgcca tgtttttcgc aatatccgcg accttcacgc atcaggcagg
     3541 aacgataagg ccatggcttt tcgcccttct gagcaaatgc aatctctgcc cgtaactcat
     3601 ccattcgcgc ctgtagtctt ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca
     3661 cctcagaatg tcagtttgtc actggattta ccgaatttgt cagacatggc tcccaggcca
     3721 gccaggacat cgacctgtcg ctgtcgccca cctctgggag cggctggctg ttgccagaag
     3781 tcttcgaagt gacgatcggg tccaaagaac gtcgcagcct gcttcacgaa ctgtgtgccg
     3841 gtatttcctg tggcacgtac ccaggcagca tagcgtttca cgccatcaag catggcttca
     3901 ggttttattc cctccctgag ccgggctttc caggctttga aggctgcgga ttttgagtta
     3961 ccaccagcac gttttggata ttcctgccag gcctgttcaa attccggtga atattcctgt
     4021 cgggaagaac gcgctggtgc agacgcgtca gcggatgcgc caatagtgtt tttactctct
     4081 gtagtattct ctgaagtaat ctctgttgta ttctctgtaa gatcgaaatt ggttttccct
     4141 tctccgcggc gaggggtttc ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg
     4201 ggttttacag tttcccgaaa acgggtttcc ccatttcggg aaaactgatt gttttcattg
     4261 ataatttcat taaggcgctc acaatctata cggtagaaca ttttgtgctc aagacgcttg
     4321 ttggtttcaa ccaaaatgcc tctggacaca agatgcttac gcgctacagc ctgttgttca
     4381 aatgtaagtc cggtttcgtg ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca
     4441 tgtgctttat cctgccagta aaaaatctga ccaaagaaaa taacagcgtg cggacttccc
     4501 atgtatttaa cgagcccagg gtaataagca accggatgtc caaaatcgag cagaagatca
     4561 gacggacgca tagccacctc ccaggcgttt aaacattttt ccgggctgaa acgccaccag
     4621 cggataactc agggtatgag tacgtccctg aacctggcag acaaccttct ggctttcggt
     4681 actgaccagg caaacccgca gaacgtagcc gttgctggtg gtgaaccact gccccacacg
     4741 ggggcaatgg ttgtatcggt gatacaggaa attaacgatg tggcggatca tgggcgcacc
     4801 tccttgtcag aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct
     4861 acacggtcgt acagtttgcc agccagcgga gattcaacgg ccttaagcat tgggtaaagc
     4921 tggcttgtcc agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgttttgt
     4981 gcgacagatg acatgtcagc cgcaccagct cctgataagc tcctctccat ctggttaaag
     5041 gcattgatgt atgcctcttt gaaccgggcg gcacgtttac cagtgaagcc catagcaagg
     5101 aaggcgaagc cgccgcgggt aatttggtag cagggaagtt tgcgagtacc gccgttgggc
     5161 tggcgtacca aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc
     5221 aaaatgcgga tctttttcag aacatcgtca tgacgtttag agaagaagtt ggcaacagcc
     5281 agggatgaag taacagccta accatcaacg atggcaattt caggttgaga gagggttggg
     5341 agagtagtca tggtgacagc ccctatgttg aattcaaaga actcaccaca tgggacgcca
     5401 atcacagagg tggtgagacg tacagggttg gcgtaaccgg tcaacataga acccggcgca
     5461 tcttgcgatg tccctgcacg ccccaccata atttgggcgt agcaatgctc atgacacgaa
     5521 aaaaccgcat gagcgcggtt atgctctata ttgaatttca ggacgccaat cccggccccc
     5581 gctttataag gtgcagagac agtgtaacgt cccgaaattg cagaatcaat atattgtgca
     5641 gatataacga ttttgttatg ttttgctgta tgaactacac tatcgaatac tacagtgaag
     5701 aggtccgact ggaggtcgat cagcttccat tgagtatgcg tgccagatac cagcatcttg
     5761 ttgaacgcat gaaggtatac ggcagcaatc tcggagaacc tcacaccagc gcctttggtg
     5821 acgggctttt cgaactcaga attaaaggca acgatgggat cgcgcgtgtt ttttactgca
     5881 ctctgacagg gaaacgcatc atcatgctgc atagttttgt aaagaaagcg cagaaaacac
     5941 cgccagccga acgcaagaaa gctgaaacca gaatgaagga ggttaagcat gactggtaaa
     6001 cgcactctcc ccaccatgac acacgacgaa atggcagcca aatggatgga agacccggct
     6061 tttaaagcag aatacgacgc tatcgctgac gaattcgcac tgcttgatga aatgctggca
     6121 gcacgcaaag aagctggctt aactcaggct gaagttgccg agcgaatggg aacaaaagcg
     6181 accgcgatca ccagaatgga aagtaatctc gcatcaggta tcagcggccc atcatttgcc
     6241 acactaaaaa aattcgcccg cgctactgga aaaaaactcc agatccgctt cgtttaacca
     6301 caccgcgccg tcattctggc ggcgcaccgg ataaatagtc atttcctcgc acgatgtctt
     6361 agccaccgga tatcccacag gtgagtcgtg tagttgaagg tttttacgtc agattctttt
     6421 gggattggct tgcgtttatt tctggagcgt ttcgttggaa ggtatttgca gttttcgcag
     6481 atgatgtcgg tgaaacttcg tcgatgtcgc ctcattcata cctcctgtcg gtaaatctga
     6541 caccctgacc aatagcccag gctgttgtgt actcgatcag acttgccatg cgcttcacgc
     6601 tcatctgcgc gctgctttcg cgaatgttga cgcattctcc ttcaaggccg ggcaaaacat
     6661 cagcttcctg ttttgttgcc actgcatgac cgctgatcaa caaaaccttc cattgttctg
     6721 gttttaacca tttgccgcac cattgaacct gacgagcgat atccgccagc atcgcgtgaa
     6781 attttgcgtt ctggtcaaga tttcgcttgt agtcagtaat gcggatggtg actggcttgt
     6841 ctttatcgag aggagttgag aggatggcat ttattgcggc ttgctgttgt tgcttagttc
     6901 ggaggaagat tgtttgcttc atcgaaaatt cttctcttta attccagcgg ctctgatagc
     6961 tttcattact gcaattaccg ttttgtcctt cccatcctca tgccccatcg cataagcacc
     7021 ttcttcacca tctttccaaa agtcgtcatt cgattcgggc cagtcgatat ccagttcaat
     7081 agctgctcgc gatgcctgcc atatcaccca ggcaaactct tttaattcat cgtctcctgt
     7141 gaactggctt ttgtcttttg accaccagtt ttcaaactgt cggtagctat cgttcacttc
     7201 cctctccccc aaataaaaag gcctgcgatt accagcaggc ctgctattag ctcagtgatg
     7261 tagatggtca tttaatactc cgtcacgttt tcctgtcgcc acgcctcgtc atattccgat
     7321 ttcggcatat tggcgatgta gctatatggc gatcctgatt caagttgcag gaactggtgc
     7381 gattgctcgt caaggaacaa cggaacacca ccttcccaac cttcgccgtt acgttgtttt
     7441 tcaagcatca aaacagatgc
//
LOCUS       DAEBXD010000152         7387 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEBXD010000152 DAEBXD010000000
VERSION     DAEBXD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.152"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            396..1967
                     /gene="garD"
                     /locus_tag="KJL94_003696"
     CDS             396..1967
                     /gene="garD"
                     /locus_tag="KJL94_003696"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBD4411881.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(2019..2843)
                     /gene="agaR"
                     /locus_tag="KJL94_003697"
     CDS             complement(2019..2843)
                     /gene="agaR"
                     /locus_tag="KJL94_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBD4411882.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            3092..4372
                     /gene="kbaZ"
                     /locus_tag="KJL94_003698"
     CDS             3092..4372
                     /gene="kbaZ"
                     /locus_tag="KJL94_003698"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBD4411883.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4395..4868
                     /gene="agaV"
                     /locus_tag="KJL94_003699"
     CDS             4395..4868
                     /gene="agaV"
                     /locus_tag="KJL94_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBD4411884.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4879..5658
                     /locus_tag="KJL94_003700"
     CDS             4879..5658
                     /locus_tag="KJL94_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBD4411885.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5648..6457
                     /locus_tag="KJL94_003701"
     CDS             5648..6457
                     /locus_tag="KJL94_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBD4411886.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6544..6978
                     /locus_tag="KJL94_003702"
     CDS             6544..6978
                     /locus_tag="KJL94_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD4411887.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6975..>7387
                     /locus_tag="KJL94_003703"
     CDS             6975..>7387
                     /locus_tag="KJL94_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD4411888.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1764 a         1892 c         1947 g         1784 t
ORIGIN      
        1 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
       61 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
      121 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
      181 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
      241 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
      301 actcctgaca ttcttatttc acccaatgaa atcatttatt tttaaatgag accaggtcct
      361 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
      421 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
      481 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
      541 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
      601 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
      661 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
      721 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
      781 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
      841 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
      901 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcagcag
      961 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     1021 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     1081 atgtgcaagc tattccagta gaaatcgcca gcattgtcag tttgcaggat gaaaagcatg
     1141 tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac
     1201 tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcattgcg
     1261 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc agttggctat gcgtctgatc
     1321 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     1381 tccatctgct gacaccacgc gcagtgaacg aagaggtcgg caaacggctg ctggaggaga
     1441 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     1501 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     1561 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     1621 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     1681 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     1741 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     1801 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     1861 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     1921 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     1981 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     2041 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     2101 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactggaa
     2161 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     2221 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     2281 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     2341 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     2401 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     2461 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     2521 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     2581 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     2641 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag atcattgcgg
     2701 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     2761 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     2821 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     2881 ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat tggcgcaagc
     2941 tactctcgtt tcagtgactt tcattacgtt tcttttgtga atcagatcag aaaactatta
     3001 tctttcgttt tatttttatc tcaccatgac gcagtatcaa ttgaaacaaa acgaaagatc
     3061 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     3121 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     3181 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     3241 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     3301 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     3361 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     3421 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     3481 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     3541 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     3601 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     3661 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     3721 gcaaggtttg aatgccatct ggccacgcat tattgccctg gtggttcaac ccggcgtcga
     3781 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     3841 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aaacgccgca
     3901 atctctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc cggcgctgac
     3961 cttcgccctg cgtgaagccc ttttttctct ggcggcgatt gaagaagaac tggtaccagc
     4021 gaaagcctgt tccggtctgc gtcaggtgct ggaaaacgtg atgctcgacc gcccggaata
     4081 ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg gttacagcta
     4141 ctcagatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt tcgctcatct
     4201 ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt atctgccgct
     4261 gcagtacgtg aaagttcgct ccggcgagct gcaaccgacg ccacgggaac tcattataaa
     4321 ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat aagcaaaaca
     4381 aagaggaaca tgctatgcca aatattgttt taagccggat tgatgaacgc ttgattcacg
     4441 gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg gtagccaacg
     4501 atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg gcagaaggga
     4561 tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc gccgccgatc
     4621 gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg gtgaaaggtg
     4681 gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc aaacaacaaa
     4741 tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac ctgaaagccg
     4801 ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac ctctttaaat
     4861 tactttgagg gattcatcat ggaaatcagc ctgttgcagg catttgcgtt gggcattatc
     4921 gcctttatcg ctggcctaga tatgtttaac ggcttaaccc atatgcaccg cccggtggtc
     4981 ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt aaccggcgat
     5041 acgctggaac tagtgtggat ggggctggcc ccgctggcgg gcgcacagcc gcctaacgtg
     5101 attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa acccgatgtc
     5161 gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac cttcctgttc
     5221 tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg cggagaacgc cgatactcgc
     5281 ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta ttttctctgc
     5341 gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga tgtcctgccg
     5401 caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat cggttttgcc
     5461 gtgctgctga aaatcatgat gaaaaacgtc tacataccct acttcatcct gggctttgtt
     5521 gccgcaacct ggctcaagtt accggtgctg gctattgctg ccgccgcgct ggcgatggcg
     5581 ctgatcgatc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca gaaagaggaa
     5641 tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agcgaaaatg aagaaacact
     5701 gctgactggc gtcaatgaaa acgtgtatga agatcggagc attgccgcgg agctgacgaa
     5761 aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt ttaactacga
     5821 acgtatgcag gcttctggct ggttgtacgg tctgctgcct gctctgaaaa agatccacac
     5881 taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca atacccatcc
     5941 gtttctggtg acgtttgtta tcggcattat ccttgcgatg gagcgttcta agcaggacgt
     6001 taacagtatt cagagcacca aaattgccgt cggtgcgccg cttggcggga ttggcgatgc
     6061 gatgttctgg ctaacgctac tgccgatttg tggcgggata ggcgcaagcc tcgcgctaca
     6121 aggctctatt cttggcgcag tcgtctttat tgtgctgttc aacgtggtac acttggggct
     6181 gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggcgattc cccttattaa
     6241 agcgaatacc aaaaaagtcg gccatgctgc gtcaatcgtt ggaatgacgg taatcggcgc
     6301 actggtggca acctatgttc gtttaagcac cacgctggaa atcacggcgg gcgacgcagt
     6361 agttaagtta caggctgatg ttatcgacaa actgatgcct gccttcttac cgctggtcta
     6421 caccctgacc atgttctggc tggtacgccg cggctagagt ccgctgcgcc tgattgcggt
     6481 taccgtggtt ctagggatcg tcggtaaatt ctgccatttc ctttaaatac aatgaggtat
     6541 tagatgttaa gtattatttt gacagggcat ggcgggtttg ccagtggcat ggaaaaagca
     6601 atgaagcaaa tcctcggcga tcaatcgcag tttattgcca tcgattttcc cgaaacctca
     6661 agcgccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga ttgtgaagac
     6721 ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc ttcaacgctg
     6781 gcgatgcaaa aaccgggctg tgaagtgatt accggcacca atttgcaact gctactggag
     6841 atggtgctgg agcgcgaagg gttaagcggt gaagaattcc gtgtacaggc gctggagtgt
     6901 ggacaccgag ggctgaccag tctggtggac gagttaggcc gctgccatga agaatgtccg
     6961 gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact gaagagggat
     7021 ggttcgatga ccatcagttg cgtgttgctg acggtgtcat cgcagcaatc gaaccgattc
     7081 cagtgggcgt gactgaacgc gatgcggaac tgctctgccc cgcttacatc gacacccatg
     7141 tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtactc gacaagctgg
     7201 caatgcacaa ggcacgcgaa ggtgtcggca gttggttacc gaccaccgta accgcgccgc
     7261 ttaataccat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc ggcggacctg
     7321 gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag aataaaggcg
     7381 cgcatcc
//
LOCUS       DAEBXD010000153         7287 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEBXD010000153 DAEBXD010000000
VERSION     DAEBXD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.153"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="KJL94_003704"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="KJL94_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBD4411889.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="KJL94_003705"
     CDS             1102..2562
                     /locus_tag="KJL94_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD4411890.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KJL94_003706"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KJL94_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBD4411891.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KJL94_003707"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KJL94_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBD4411892.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KJL94_003708"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KJL94_003708"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBD4411893.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="KJL94_003709"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="KJL94_003709"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBD4411894.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="KJL94_003710"
     CDS             complement(6727..7245)
                     /locus_tag="KJL94_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBD4411895.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1795 a         1725 c         1870 g         1897 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgcttt
//
LOCUS       DAEBXD010000154         7184 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEBXD010000154 DAEBXD010000000
VERSION     DAEBXD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.154"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(296..1198)
                     /locus_tag="KJL94_003711"
     CDS             complement(296..1198)
                     /locus_tag="KJL94_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBD4411896.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KJL94_003712"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KJL94_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBD4411897.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KJL94_003713"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KJL94_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBD4411898.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KJL94_003714"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KJL94_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBD4411899.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KJL94_003715"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KJL94_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBD4411900.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KJL94_003716"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KJL94_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7184
                     /locus_tag="KJL94_003717"
     CDS             7090..>7184
                     /locus_tag="KJL94_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBD4411901.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1673 a         1811 c         1878 g         1822 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgct
//
LOCUS       DAEBXD010000155         7012 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEBXD010000155 DAEBXD010000000
VERSION     DAEBXD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.155"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(42..126)
                     /locus_tag="KJL94_003718"
     tRNA            complement(42..126)
                     /locus_tag="KJL94_003718"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Tyr,seq:gta)
     gene            complement(286..1128)
                     /gene="purU"
                     /locus_tag="KJL94_003719"
     CDS             complement(286..1128)
                     /gene="purU"
                     /locus_tag="KJL94_003719"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBD4411902.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1178..1636)
                     /locus_tag="KJL94_003720"
     CDS             complement(1178..1636)
                     /locus_tag="KJL94_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBD4411903.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1749..2654
                     /gene="rssA"
                     /locus_tag="KJL94_003721"
     CDS             1749..2654
                     /gene="rssA"
                     /locus_tag="KJL94_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBD4411904.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2746..3759
                     /gene="rssB"
                     /locus_tag="KJL94_003722"
     CDS             2746..3759
                     /gene="rssB"
                     /locus_tag="KJL94_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBD4411905.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3961..4869
                     /gene="galU"
                     /locus_tag="KJL94_003723"
     CDS             3961..4869
                     /gene="galU"
                     /locus_tag="KJL94_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBD4411906.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5013..5426)
                     /gene="hns"
                     /locus_tag="KJL94_003724"
     CDS             complement(5013..5426)
                     /gene="hns"
                     /locus_tag="KJL94_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBD4411907.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6031..6648
                     /gene="tdk"
                     /locus_tag="KJL94_003725"
     CDS             6031..6648
                     /gene="tdk"
                     /locus_tag="KJL94_003725"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBD4411908.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1846 a         1530 c         1723 g         1913 t
ORIGIN      
        1 attgcagatt cgtttgagaa ttccggggct tttgaaagtg atggtggtgg gggaaggatt
       61 cgaaccttcg aagtcgatga cggcagattt acagtctgct ccctttggcc gctcgggaac
      121 cccaccacgg ggtaatgctt tttactagcc tgctccctta tcgggaagcg gggcgcatca
      181 tatcaaatga cgtgccgctg taaagtgtta cgttgagaaa aatgaactgg ttgcgtaatt
      241 ttcatccgta acggattaaa ggtaaccagt tatttttgct ggcgattaaa gaataatcgt
      301 ccgattaccg taaacaaaga cgcgttgcgc cagtactttg tatagcgcac gacttaagac
      361 gtttttctcg acgtcacgac ctgcgcgcat catatcttca gctgtgtagg tatgatcgac
      421 atgaataacg tcctgcatga tgattgggcc ttcgtccaga ttgtcattca catagtgagc
      481 ggttgcgcca ataatcttca caccacgttc ataggcctgg tgataaggac gtgcgccgat
      541 aaacgcaggc aggaatgaat ggtgaatatt gatgatttta ttcgggaagc gtgccacaaa
      601 ttccggcgtt aatacccgca tatacttcgc cagcaccaca tagtcaggtt gataagcatc
      661 aatggcgtcc gccatctttt gatcgtgctc gttgcggctt aacccttcat ggcttaccag
      721 ctcaaacgga atatcaaaac gctcaaccag agaacgtaaa gtatcgtggt taccaataac
      781 tgccgcgatt tcgacatcca gaccgccgta attggctttc atcaacaaat cgccaaggca
      841 atgcgcttct ttagtgacca gaatcactat ccggcgacga ccggcaggat tcagctcacg
      901 cacggagcct tctggcaatg cgctatcgag atccgccagc agggtggaat cattaaaaat
      961 cccttccagt tccgtgcgca taaaaaagcg cccggtacgg tgatcaacaa attcattgtt
     1021 ctgtacgata tttaactcgt gcttgtagca aatattggta atacgtgcga tcagaccttt
     1081 ttggtccgga caaatagtac gcagaacttt acgttggagt gaatgcattg ctggaaaaac
     1141 cttgttgaga gtgtttgcta aaccgtaccg tcaaccatta ttggccgcag cactttttaa
     1201 attttttacc tgaaccacaa gggcagggat cgttgcgacc aaactgcgga cgtgtaccgt
     1261 caatatagta ccattgaccg ttttctttca aaaatcgcga acgttcaatt attgcacctg
     1321 ttttgccacc ttcggtaaat cgagcaacaa aactgacaaa tccgatattg tcggcgtcct
     1381 gccaacaatg ttcaaatacg gtcaatccca accattcagt atgcgcaaat ccggtcatca
     1441 attcggcacg taacgccgct gctccacaag agggatgcca ggtctttatt aaataatcag
     1501 cgtcttgcat cacaaaagcg cagtaacgcg aacgcatgag atgttctgga tcaggtgcaa
     1561 ccttttcacc agacacataa gggtggcaac ataggctata ctcgacagca ctaccacagg
     1621 gacaaagctg agacacaaat aatctccctg gaaacaataa cggcgtatta accgcctgag
     1681 tagcactatg ttaaccgagc agtagcgatg tggctacgat tgcattccag gggaatcttg
     1741 cgggaataat gagaaagata aaaatagggc tggcgctggg atctggcgcg gcgagaggtt
     1801 ggtcgcatat tggcgttatt aatgcgctaa aaaaagtggg tattgaaatt gatatcgttg
     1861 caggatgttc aattggttcg ctggtgggcg cggcctatgc atgcgatcga ttatctgcgc
     1921 tggaagattg ggtaacctct ttcagttatt gggacgtttt acgcctgatg gacctctcct
     1981 ggcagcgcgg tgggttactg cgcggcgagc gtgtcttcaa tcaatatcgc gaaataatgc
     2041 cggaaacaga gatcgaaaat tgttcccgtc gctttgcggc tgttgccacc aatttaagta
     2101 cggggcgtga attatggttt actgaaggcg atctccatct tgctattcgc gcatcatgca
     2161 gtattccagg actcatggca cccgttgcac ataacggcta ctggctggtt gatggggcag
     2221 tagttaaccc aattcctatt tctcttacgc gtgcattggg ggctgatatt gtgatagcgg
     2281 tcgacctgca gcacgatgct catttgatgc aacaagatct gctctccttt aatgtcagtg
     2341 aagaaaatag cgagaatggt gattctctac cgtggcatgc gcgtctgaaa gaaaggttag
     2401 gcagcataac gacacgtcgg gctgtgacag cgccaacggc aacagagatt atgaccacgt
     2461 ctatccaggt gttggagaac cgccttaaaa ggaaccgcat ggcaggtgat ccgcccgata
     2521 ttctgattca acctgtttgc ccgcaaatat ctacgcttga tttccatcgc gcgcacgctg
     2581 ccattgcggc cggacagctg gcagtggaaa ggaaaatgga cgaacttttg ccgttggtac
     2641 gcaccaacat ttgaccagaa tttttatcta cacttaagtt aattctgaca ggcgcaggtg
     2701 gcaatagcat gccactattg agtaaagcca gtcaggggag agaacatgac gcagccattg
     2761 gtcggaaaac agattctcat tgttgaagat gagcaggtat ttcgctcgct tctggattca
     2821 tggttttcct cattgggagc gacaacggta ctggcggctg atggggtgga tgcccttgag
     2881 ttgctgggag gtttcactcc agacctgatg atatgtgata tcgcgatgcc acgaatgaac
     2941 gggcttaaac tgctggagca tatacgtaac agaggcgacc agaccccagt tctggtgata
     3001 tctgccactg aaaatatggc agatattgcc aaagcgttac gtctgggcgt tgaagatgtt
     3061 ttgctgaaac cagttaaaga tctgaatcgc ttgcgcgaga tggtttttgc ctgtctctat
     3121 cccagcatgt ttaattcgcg cgttgaggaa gaggaaaggc tttttcgcga ctgggatgca
     3181 atggttgata accctgccgc agcggcgaaa ttattacagg aactacaacc gccggttcag
     3241 caggtgattt cccattgccg ggttaattac cgtcaattgg ttgccgcgga caaacccggc
     3301 ctggtgcttg atattgccgc actttcggaa aacgatctgg cattttattg ccttgatgtc
     3361 acccgagctg gacataatgg cgtacttgct gccttgttat tacgcgcatt gtttaacgga
     3421 ttattacagg aacagcttgc acaccaaaat caacggttgc cagagttggg cgcgttattg
     3481 aagcaggtaa accatttact tcgtcaggcc aatctgccgg ggcagtttcc gctattagtt
     3541 ggctattatc atcgcgaact gaaaaatctc attctggttt ctgcgggtct gaatgcgacg
     3601 ttaaataccg gcgaacacca ggtgcaaatc agtaatggtg ttccgttagg cactttaggt
     3661 aacgcttatt tgaatcaatt gagccagcga tgcgatgcct ggcaatgcca aatatgggga
     3721 accggtggtc gactgcgctt gatgttgtct gcagaatgag caaacgataa cgcgggctaa
     3781 atttgcatta cctgctaatg tcggctggtg gtactatcgt cgccattcgt ataagtaatt
     3841 gtcttaatta tgctatctcg cctccttttc agaacttagc cccttcgggg tgctgatata
     3901 ctgggatgcg atacagaaat atgaacacgt tcaaaacacg aacagtccag gagaatttaa
     3961 atggctgcca ttaatacgaa agtcaaaaaa gccgttatcc ccgttgcggg attaggaacc
     4021 aggatgttgc cggcgacgaa agccatcccg aaagagatgc tgccacttgt cgataagcca
     4081 ttaattcaat acgtcgtgaa tgaatgtatt gcggctggca ttactgaaat tgtgctggtt
     4141 acacactcat ctaaaaactc tattgaaaac cactttgata ccagttttga actggaagca
     4201 atgctggaaa aacgtgtaaa acgtcaactg cttgatgaag tgcagtctat ttgtccaccg
     4261 cacgtgacta ttatgcaagt tcgtcagggt ctggcgaaag gcctgggaca cgcggtattg
     4321 tgtgctcacc cggtagtggg tgatgaaccg gtagctgtta ttttgcctga tgttattctg
     4381 gatgaatatg aatccgattt gtcacaggat aacctggcag agatgatccg ccgctttgat
     4441 gaaacgggtc atagccagat catggttgaa ccggttgctg atgtgaccgc atatggcgtt
     4501 gtggattgca aaggcgttga attagcgccg ggtgaaagcg taccgatggt tggtgtggta
     4561 gaaaaaccga aagcggatgt tgcgccgtct aatctcgcta ttgtgggtcg ttacgtactt
     4621 agcgcggata tttggccgtt gctggcaaaa acccctccgg gagctggtga tgaaattcag
     4681 ctcaccgacg caattgatat gctgatcgaa aaagaaacgg tggaagccta tcatatgaaa
     4741 gggaagagcc atgactgcgg taataaatta ggttacatgc aggccttcgt tgaatacggt
     4801 attcgtcata acacccttgg cacggaattt aaagcctggc ttgaagaaga gatgggcatt
     4861 aagaagtaac atccgtatcg gtgttatcca cgaaacggcg ttgagcaatc gacgccgttt
     4921 ttttatagct tattcttatt aaattgtctt aaaccggaca ataaaaaatc ccgccgctgg
     4981 cgggatttta agcaagtgca atctacaaaa gattattgct tgatcaggaa atcgtcgagg
     5041 gatttacctt gctcatccat tgcttttttg attacagctg gagtacggcc ttggccagtc
     5101 caggttttag tttcgccgtt ttcgtcaacg tagctatatt ttgccggacg ctgagcacgt
     5161 ttagctttgg tgccagattt aacggcagca aggctattca gcagttcgtt cgggtcaata
     5221 ccgtcagcga tcagcatttc gcgatattgc tgcagcttac gagtgcgctc ttcaacttca
     5281 gcagcagccg cgctttcttc ttcgcgacgt tcgttaacaa caacttctaa tttttccagc
     5341 atttcttcca gcgtttcaag tgtacattct cttgcctgcg cacgaagagt acggatgttg
     5401 ttcagaattt taagtgcttc gctcattgta gtaatctcaa acttatattg gggtggtttg
     5461 ttgaggtaat aatagagcct taaattcagt tgtgcaatag ccaggaatgt aaggaattca
     5521 aaattgttct ttattttgtg ccgccaataa atatcttttc ataaaattag ccagaaaaga
     5581 cgcggcatat agccctattt acaccgatga tttcgcagca cgtgaggtta aaacttcctg
     5641 attcatgtca cattttatgg ggagattatc gtaggctgac gacctttcag tcttctgtat
     5701 tagttgtgtt tacgagaatt ccctattaag cgaatgatga aaagtagaac agtcgcaata
     5761 agagcatgga cttagtattg cactatctcc tggaggtcaa cagagggcta ttacttgcgc
     5821 aacaggttaa agattgtgaa tagttaccag cagtcattta cccgcttata acaagcgagg
     5881 cagttgtaat gatagctcag aaggattatg caaggcttcg taagggagaa cgcatatacc
     5941 cacttctgtg catactgttg agctgaaaaa ctgacgaatt atgataaact ccagccaact
     6001 ttatttcata tcattgaggg cctgtggctg atggcacagc tatatttcta ctattccgca
     6061 atgaatgcgg gtaagtctac agcattgttg caatcttcat acaattacca ggaacgcggc
     6121 atgcgcactg tcgtatatac ggcagaaatt gatgatcgct ttggtgccgg gaaagtcagt
     6181 tcgcgtatag gtttgtcatc gcctgcaaaa ttatttaacc aaaattcatc attatttgat
     6241 gagattcgtg cggaacatga acagcaggca attcattgcg tactggttga tgaatgccag
     6301 tttttaacca gacaacaagt atatgaatta tcggaggttg tcgatcaact cgatataccc
     6361 gtactttgtt atggtttacg taccgatttt cgaggtgaat tatttattgg cagccaatac
     6421 ttactggcat ggtccgacaa actggttgaa ttaaaaacca tctgtttttg tggccgtaaa
     6481 gcaagcatgg tgctgcgtct tgatcaagca ggcagacctt ataacgaagg tgagcaggtg
     6541 gtaattggtg gtaatgaacg atacgtttct gtatgccgta aacactataa agaggcgtta
     6601 caagtcggct cattaacggc tattcaggaa aggcatcgcc acgattaata agaatttctt
     6661 tactgacagg gtgagcaggg cacttttatc ctgtcagttc gttttacgca cttcttccgg
     6721 gctatatacc cttctcggca gttttttaac gccgctatac gcctcacagg gctcttaagc
     6781 accgacgttg acttgtgacc tgtaaagtac aatatccctg tgtttaggcg ttatacatcg
     6841 tcgcaaatat gatgaaggct aatgctgtcg gtttatggaa aagttgcttt gggtaaacaa
     6901 aaaaacggcc ccagaagggg taatgccagt cagttaagca actgactggc tctttttcgg
     6961 agctgtgggg tatttccagg gcctctcctt taccactctc gggaaggccc tt
//
LOCUS       DAEBXD010000156         6875 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEBXD010000156 DAEBXD010000000
VERSION     DAEBXD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.156"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..195)
                     /locus_tag="KJL94_003726"
                     /pseudo
     CDS             complement(1..195)
                     /locus_tag="KJL94_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(305..1075)
                     /gene="yafV"
                     /locus_tag="KJL94_003727"
     CDS             complement(305..1075)
                     /gene="yafV"
                     /locus_tag="KJL94_003727"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBD4411909.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1256..1702
                     /gene="ivy"
                     /locus_tag="KJL94_003728"
     CDS             1256..1702
                     /gene="ivy"
                     /locus_tag="KJL94_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBD4411910.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1745..4189)
                     /gene="fadE"
                     /locus_tag="KJL94_003729"
                     /pseudo
     CDS             complement(1745..4189)
                     /gene="fadE"
                     /locus_tag="KJL94_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            4429..5007
                     /gene="lpcA"
                     /locus_tag="KJL94_003730"
     CDS             4429..5007
                     /gene="lpcA"
                     /locus_tag="KJL94_003730"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBD4411911.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5213..5980
                     /locus_tag="KJL94_003731"
     CDS             5213..5980
                     /locus_tag="KJL94_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBD4411912.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(5951..6691)
                     /locus_tag="KJL94_003732"
     CDS             complement(5951..6691)
                     /locus_tag="KJL94_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBD4411913.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         1647 a         1868 c         1747 g         1613 t
ORIGIN      
        1 tccccttaac tggctgttta acctgataaa gttccctgcg cagttcctgt acttcgttgt
       61 tatcccaagc aggatcaaac atccctccga gatcttcacg gtaacgcccg tgccgatctc
      121 aaatcccgct tttctcagcc atttggcgct gattttgatc accggcgtgt cgctgcggtt
      181 gggcagataa cccacggtac agcgacgctg tgttttggaa atggtgactt ctggcgtaat
      241 attgtgttta agttaggggc tttcgtaatc tagcccagcc cccggcccta aacacaaccc
      301 ccactcacca caacctaaac tcatccgcat cccgccatgc cggaaacttt tctctatatt
      361 cccgcaatgc caccatcgac agctccgcat caatacgcgt tgccttatgc gcgtcggcgg
      421 tagcgataat ctcgccttgc ggattaatca cccggctgtc accgcgataa tggcagccgt
      481 tgccatcgct gccgacgcga ttgcatcccg ccacatacgc ctgattctca atcgcgcggg
      541 ccgtcagcaa tgcctgccag tgcagagagc gcggagctgg ccagttggcg acgtacaggg
      601 cgaggtcata atcgttgaga ttgcgcgacc acactggaaa acgtaagtcg tagcacacca
      661 gcggcaaaat acgccagccg cgccattcca caatcactcg cgcattgccc gctttataat
      721 gcagatgctc atctgccatg cggaacagat gacgcttatc ataaaaatgt accgtgccgc
      781 ccggctcaac cagcaaaaag cggttaaccg aaccagactc cgtttgtaat gcaacactgc
      841 ctgcaatcag cgcattgcac tgctgcgcct tagctgtcat ccagttcact acgtcatctt
      901 gtgcaagcga cgaagctgcc gcttccatgg caaagccgct ggtaaacatc tccggtagaa
      961 cgatcacatc gcgcccggta ataccttcca gttgacgatc aaaatggcgc aggttggcag
     1021 gaccatccac ccacaccaac ggttgctgca aaagcgtaat cttcaaacta ggcacggtgt
     1081 acaactcctt tatgcgaagg gttttataac tttaacacct tatcaggcag ttgccttagc
     1141 gcagaataaa ttgataacaa atgctgatat tggaaatatc tgatttgcaa attatcgtgt
     1201 tatcgccagg ctttaggagg ttaataacat gggcaggata agctcgggag gaatgatgtt
     1261 taaggcaata acgacagtcg ccgcactggt catcgccacc agtgcaatgg cgcaggatga
     1321 tttaaccatt agcagccttg caaagggcga aaccaccaaa gctgcgttta atcagatggt
     1381 gcaagggcat aagctacctg catgggtgat gaaaggcggt acttatactc ccgcacaaac
     1441 cgtaacgttg ggagatgaga cgtatcaggt gatgagcgcg tgcaaaccgc atgactgtgg
     1501 ctcgcaacgt atcgctgtga tgtggtccga gaaatctaat cagatgacgg ggctgttctc
     1561 ggctattgat gagaaaacgt cgcaagagaa actcacctgg ctgaatgtga acgatgcgct
     1621 ttcgattgat ggtaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg aaaaccatcc
     1681 ggatggcttt aattttaaat aattagcgga taaagaaacg gagcctttcg gctccgttat
     1741 tcaattacgc ggcttcaact ttccgcactt tctccggcaa ctttaccggc ttcgtcgcca
     1801 gctcttccgg atcaaagtca tcaacgttaa tactgcgcag acggctttct tccgctttca
     1861 ccagaatagc ggcttcatct ttatcaatca gccccttcgt cagcgcgttg tgcgccagtt
     1921 catccagacg ggtaaatggc agatttttac ccagctcttt acagatccgc tgatgaatcg
     1981 ggtcggcggc aatcacatcc accaacgcct cttccagtaa accaaccggg ttatgctcgc
     2041 ttggcgtcag gtactgaccg cgaccaatac gtgaacgagt ggcgttcggc acttgtaaaa
     2101 tcttcgccac tttatgatcc agcttgtcag acggtgccag ataatgacgt ccggtcggga
     2161 agatcaccac attcagcagc ccggcaacca cgcggttcgg gaagttttgc agtaaattgt
     2221 ccattgcctg ttctgcctga tacagcgcat cctgtacgcc ccagtgcacc agcggcagat
     2281 cggcttcatt acggccttcg tcgtcataac gcttcagaac ggcagaggcg aggtaaagct
     2341 ggcttaaaat atcccccaga cgtgccgaga tacgctcacg acgtttcagg ctgccgccca
     2401 gcagtgccat cgagacatca gaaagcaggg cgaggttggc gctcaggcgg ttcaggtgct
     2461 gatagtagcg tttagtggca tcgccggttg gcgtgctgct ggttaaaccg cgcgtcaggc
     2521 ccagccagaa gctgcgaact ttgttgctac cgacgtgacc gatatgtttg aacaacagtt
     2581 tatcgaacgc gttgacgtca ttgttcttcg ccgcttccat ctcttccagc acgtacggat
     2641 ggcaacgaat cgctccttgt ccgaagatca tcatgctgcg ggtcagaatg ttagcccctt
     2701 caacggtgat ggcaatcggt gcgccctggt aagcacgcgc caggaagttg ctttgcccga
     2761 gcataatgcc tttaccgccg gtaatatcca tcgcatcaat aatcgactgc tgcccgcggt
     2821 gggtacagtg atacttaacg atagccgaca gcacggcagg tttttcgccg agcataatgc
     2881 cgtaggtaat cagcgatgcc gcagcatcca ttacataagc gttcccggca atacgcgcca
     2941 gcggctcttc aatcccttcc atcttaccga tagagatttt gaactgacgg cgaatgtgag
     3001 aatacgcgcc cgttgccagc gctaccgatt tcacgccgcc ggttgagttg gaaggcaggg
     3061 tgatgccgcg gcctaccgag aggcactcca ccagcatccg ccagccttgc ccggccattt
     3121 tcggcccgcc gatgatgtaa tcgatcggca cgaagacatc tttaccgcgc gtcggtccgt
     3181 tctggaacgg tacgttcagc gggaagtggc gacgaccaat ttccacgccc ggcgtggtgg
     3241 ttgggatcag cgcacaggta atgcctaaat cttctgcacc gccgagtaat ttttccgggt
     3301 cggagagttt aaacgccagc ccaagcacgg tcgcaatcgg tgccagcgta atgtagcgtt
     3361 tgttccaggt cagacgcatc cccagcacct gctggccctg ccattcgccc atgcagacaa
     3421 tcccggtgtc cggaatcgcg cccgcatcgg aacccgcttc cgggctggtc agtgcaaagc
     3481 aggggatctc ctgaccacgc gccagacgcg gcagatagtg atctttctgc tcgtcagtgc
     3541 cgtaatgttg caacagttcg cccgggccta atgagtttgg cacgccgacg gtaatcgcca
     3601 ggatcccgct cacgccagag agtttttgca gcacacgaga ctgggcataa gctgagaact
     3661 ccagtccgcc gtactctttt ttgataatca tcgcgaagaa gcgatgctct ttaaggtacg
     3721 cccacaactc cggcggcaga tccgccagct catgagtaat ctggaaatca ttcgccatcc
     3781 ggcaggcttc ttctaccggg ccgtcgagaa acgcttgctc ttcagcggtc aggcgcggct
     3841 gcggatagtt atgcagcttt ttccagtccg gcttgccctg gaacaagtcg ccctcccacc
     3901 aggtggtgcc cgcatcaatc gcttctttct cagtgcgcga catcggcggc atcaccttac
     3961 ggaaaccgcg aaataccggc gcggaaatca tcgacttacg cataggcgca aagttaaatg
     4021 gcacgaggat aatggtcaga ggcaccagta cccacgccga ccacagacca gcaacgccga
     4081 gggcggctgt ctaggcgagc aaaatcagac tgctgataaa taagctcacg cggtgataga
     4141 acaacgcgcc gagcaggaca accgtagcga gaatactcaa aatcatcata acgaaaagcc
     4201 ccttacttgt aggaggtctg accacttgtg atgatatggt tgtagtggat gtaaaaacat
     4261 ttagcaatgt gtttacaata taattacaac aaagctcaca ttgttgctgt ttttatccgc
     4321 acttcaggtc aaaaagtcct ggtcatagca cctgcccgta cttctcgctt ttggcggtat
     4381 ccggtacact gcattttgtc tattacattt atgctgaagg atatcctcat gtaccaggat
     4441 cttattcgta acgaactgaa cgaagcggcg gaaacgctgg ctaacttttt aaaagatgac
     4501 gccaatattc acgccattca gcgcgcggcg gtcctgttag cagacagctt taaagccggt
     4561 ggcaaagtgc tttcctgcgg caacggcggt tcccattgcg acgctatgca ctttgccgaa
     4621 gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc tatttctgac
     4681 gttagtcata tttcctgcgt cggtaatgat ttcggtttca atgatatttt ctcccgctac
     4741 gttgaagcgg taggtcgcga aggcgatgta ctgctgggga tctccacctc cggtaactct
     4801 gcaaacgtga tcaaagcgat cgcagcggcg cgtgagaagg ggatgaaagt gatcaccctg
     4861 accggtaaag acggcggtaa aatggctggc acggcggata tcgaaattcg cgttccgcac
     4921 tttggttatg ccgaccgcat tcaggagatt cacattaaag tgatccatat cctgatccag
     4981 ttaattgaaa aagagatggt taagtaagtc tggcgtaggc cggataaggc gtttacgccg
     5041 catccggcac ttgtgcgctg atgcctgatg cgatgctgac gcgtcttatc atgcctacaa
     5101 atctgtaccc gaaccgtagg ccgaataatt cgtttacgcc gcatccgacc tgaaaattct
     5161 taaatcaatc ttcgccgggg tccatgcgct cccgctgttg tggaggttac ccatgtgcga
     5221 attgctcggg atgagcgcca acgtccctac cgatatctgc tttagtttca ccggacttgt
     5281 acagcgtggt ggtggaaccg ggccacataa agatggctgg ggcattacct tttacgaagg
     5341 taaaggctgt cgcacattta aagatccaca acccagcttt aattccccca tcgccaaact
     5401 tgtccaggac tacccgataa aatcctgttc ggtggtggct catattcgcc aggctaatcg
     5461 gggcgaggtg gcgctggaaa atactcaccc atttacccgc gagttatggg ggcgtaactg
     5521 gacttatgcc cataacggac aactgacggg ctacaaatca ctggaaaccg gcaacttccg
     5581 cccggtcggt gaaaccgaca gcgaaaaagc cttttgctgg ctgctgcata aattaacgca
     5641 gcgttacccg cgcacgccgg gcaacatggc ggcagtgttt aaatatatcg cctcactggc
     5701 ggatgaactg cgggcgaagg gcgttttcaa catgctgctt tcggacgggc gctatgtaat
     5761 ggcgtattgc tcgactaatt tacactggat cacccgccgc gcgccgtttg gcgtggcaac
     5821 gttgctggat caggatgtgg aaatcgactt cagctcgcag accacaccga atgatgtggt
     5881 cacggtgatt gcgacacagc cgctgacggg caatgaaacc tggcaaaaaa ttatgccagg
     5941 cgaatggcac ttattttgcc tcggggagcg tgtagtttga tgccagttgt ggctgcacca
     6001 cttcgtggct taacggcttg ctgaccacgt aacggccatt ggccacagaa acggtcggtg
     6061 gcttacgggt ttgctcaaag tagtcgtagc ccggcttcag ctgctcccag aagtccttaa
     6121 agttggaata tttatggcgc ttcatattgg cgtcggtcat gcggaatggg taaatactca
     6181 cctgcacgct cggctgacca aacaccagcg caccagtaac gaactggaag atctcatcaa
     6241 taccctgatt ggtcattgcg tagcagccga tggatacaca atcgccgtgg atcatcaggt
     6301 atttcccttc ataaccatgc gcacggtcat aagcattggg gaatccaata ttaatcgctt
     6361 tgtagtaacg gctgtctggt tttaactgat tacgctggac gctataaaac ccttccgggc
     6421 ttttgaaatc gccctgacgc tgttttggcc ctaagccgcc ggaatattta cagattttat
     6481 aactgtcgag cagttgatat tgctcgccca ttttgacgta aagatcgagc gtacgttctt
     6541 ccttgaagat ctggatgtag acaggggatc ccatcaactg ctgcttatac tctttgctca
     6601 ctggcgtggt cgagctactg ctgcccagca aaccggcaaa cgaaacgcac gggatcaaca
     6661 acatcgcaag aattaatgcg attttacgca tactgcttat tccttgataa aacggttaca
     6721 cacgccagga cggcaaaatg gatcccaaat cggaatagtc tggatttgga aggctcacat
     6781 tatcaccaaa agagttttac gcaagcctgt cggcgctggg tttacaaatt tagcacaggg
     6841 gcagttttca atagtccgaa aagcgcatgt atcat
//
LOCUS       DAEBXD010000157         6827 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEBXD010000157 DAEBXD010000000
VERSION     DAEBXD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.157"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..327
                     /locus_tag="KJL94_003733"
     CDS             <1..327
                     /locus_tag="KJL94_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HBD4411914.1"
                     /translation="NLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVRPEAITLSD
                     RGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYLEIHPYGMF
                     VLADAA"
     gene            complement(436..1368)
                     /gene="mmuM"
                     /locus_tag="KJL94_003734"
     CDS             complement(436..1368)
                     /gene="mmuM"
                     /locus_tag="KJL94_003734"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HBD4411915.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAKLLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(1355..2758)
                     /gene="mmuP"
                     /locus_tag="KJL94_003735"
     CDS             complement(1355..2758)
                     /gene="mmuP"
                     /locus_tag="KJL94_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HBD4411916.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            2966..3982
                     /locus_tag="KJL94_003736"
     CDS             2966..3982
                     /locus_tag="KJL94_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HBD4411917.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
     gene            4210..4527
                     /locus_tag="KJL94_003737"
     CDS             4210..4527
                     /locus_tag="KJL94_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HBD4411918.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(4477..4890)
                     /locus_tag="KJL94_003738"
                     /pseudo
     CDS             complement(4477..4890)
                     /locus_tag="KJL94_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(4814..5173)
                     /locus_tag="KJL94_003739"
     CDS             complement(4814..5173)
                     /locus_tag="KJL94_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
                     /protein_id="HBD4411919.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQVRLGHVVLA
                     GQQAYHESTGNGMGNPW"
     gene            complement(5201..5380)
                     /locus_tag="KJL94_003740"
     CDS             complement(5201..5380)
                     /locus_tag="KJL94_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HBD4411920.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(5385..5765)
                     /locus_tag="KJL94_003741"
     CDS             complement(5385..5765)
                     /locus_tag="KJL94_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBD4411921.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(5765..5986)
                     /locus_tag="KJL94_003742"
     CDS             complement(5765..5986)
                     /locus_tag="KJL94_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBD4411922.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            6169..>6827
                     /locus_tag="KJL94_003743"
     CDS             6169..>6827
                     /locus_tag="KJL94_003743"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBD4411923.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     N"
BASE COUNT         1756 a         1916 c         1675 g         1480 t
ORIGIN      
        1 aacctgttcc cggcaacctt cagcgacgga tacgttgata tctacggcta tcatctgccg
       61 cgcccgctgc actttggtac acagggtgaa gggatggtcg gtgtgcgccc ggaagcgatc
      121 acgctcagcg atcgcggcga agagagccag cgctgcgtga tccgccatgt cgcctatatg
      181 gggccgcagt atgaagtgac ggtggaatgg cacgggcagg agatattatt gcaggtcaac
      241 gctacgcgtc tgcaaccgga cgtcggcgag cagtattatc ttgaaatcca tccgtacggc
      301 atgtttgttc tggcggatgc ggcataacgt tttgtggttt tgtaggccgg ataaggcgct
      361 tgtgccgcca tccggcaatg cctgatgcgc tgcgcttatc aggcctacaa tgaacgatgt
      421 agacccgata aaccctcagc ttcgcgcttt taacgcggcg atatccgcag gcgtggtgcg
      481 acagcacccg ccaatcaacc gtgcgccagc ggcctgccac tgcggcagat aatccgccag
      541 ctgcgcgcaa tgttcaccgt gatgatgcca ggttttgctc acggcatcgt aatgctcgcc
      601 cgagttcgga tacaccacca gcggcagcac cgttaaaccg tgtaaatgct gcaacgcagc
      661 ggtggtgttt tccagcgcaa tacagttaat gcctagcgcc accacctgcg gataacccgc
      721 cagcaacgca accacgtcac gcagcggcgt accgtcgctc aggtgttcgc tgtcgcgcag
      781 ggtaaatgag aaccacgccc gcgcacgcgg atatgcggtc aacagcttgg ccaacgcctc
      841 aatctcggaa aaattcggca gggtttcgca ggccagcaga tcggccccgg catccagcaa
      901 ggcttccacg cgcgggcgat gaaacgcctg aaatgcctca acgctacaat gataatcgcc
      961 acggtattca gagccatccg ccagatacgc gccgtaaggc ccgacggatc ccgccaccag
     1021 aagcgttccc gcctgcgggt tctctgccag atacgcttca cgggctttac gcgccagctc
     1081 cacgcttttg ccaatcagcg ctttcgactg cgcttcatcc agacctcgcg cggcgaagcc
     1141 cgccggcgtc gcctgatagc tggcagtgat cgcgcattgc gcccccgccc ggtagtaatc
     1201 aagatgcact tcgcggataa gctccgggtt ttctaccagc actttggctg accacaggct
     1261 gtcggctaag ttacaccctc gcgcttccag ctccgtcgcc atcgcgccat ccagcagcag
     1321 gatatcctgt ttatcaagaa gagcgcgtaa cggattattc tgcgacatgt tctggctcct
     1381 gttttgcgtt tcggggttga gtaaggaaat aagcaccata gcacaacgca acaaacggta
     1441 acccgcacca caacgcaatt ctctgcgctg gatcgaatgc cagcccaaca caggccacca
     1501 ggcacagcac aaaacctaat actggcacca gcggatacca cggcgcgcga taatgtaatt
     1561 cactcaatgc cttaccttgt tgcagatgac ggcgacgaaa aacaaaatgc gaggcgcaga
     1621 tactcagcca caccgctacc accgcaaacc cggagattgc cgacagcgca acaaataccg
     1681 tgtccggggc caccacgctg gaaaacagcg ccagcacacc accgagcata ctgaccgaca
     1741 gcgccgtcag tggcacgccg tttttcgtta ctcgcgcaaa acaggccggt agcgtacgtt
     1801 cattcgacaa cgaccacagc atgcgcccgg aggcatataa cccggagttc gctgcagaaa
     1861 gaatagccgt caggatcacg aagttaaaaa tatcagcggc gtacgggatc cctactttct
     1921 caaataccag cacaaacggg cttttctcca cgcccgcctg ctgcatcggg atcagcgctg
     1981 ccagcacaaa cacggtgccg ataaagaaaa taatcagtcg cgcgatggta gtacgaatcg
     2041 ctaccgggat aactttgcgc gggttttccg tttcaccggc ggcaatgccg ataagctcgg
     2101 tacccgaaaa agcaaagttc actgccacca tagtcatcaa aatcggtaag ccaccgtgcg
     2161 ggaaccagcc ttctgccgtg atattactca gccccggcgc gggcgagcca tcctgcatcg
     2221 gaataaagcc gaaaatcgcc gccccaccga ggatgataaa ggcgatgata gtgaccactt
     2281 tgaccagcga gaaccagaac tccccttcgg caaaaaagcg cgtggagata acattcagac
     2341 caaaaataat cgcgcagaac accacgcacc agacccatac cggcacctgt ggaaaccagt
     2401 actgcataca gaatccagcg gcggtaaagc tcgaacccag cgccacggtc caggtcagcc
     2461 agtaaagcca ggccacggta tacccggtag ccggaccaag atagcgcgcg gcataaacgt
     2521 gaaacgctcc ggtctccggc atcgcgaccg acagctcgcc cagacactgc ataaccagcc
     2581 agaccaccag cgcaccaatc agataggcca gcagcgttcc cgccgctcca gtggtggaaa
     2641 tgatgtaccc ggtattgaag aataatcctg tgccaatcac gccgcccaag gaaagcataa
     2701 tcaggtgacg cgttttcatt gtgcgcttca gtggcgcatt ttgttgtgtt gtttgcatat
     2761 aaccttctaa acgtatggac atctaaacat ccaaagaagg tttgttatac cgcgtttgtt
     2821 aacagaatgc aaagtcaacc ctgcaccaga cgcagatgcc cgcgccgggc gcttaatagc
     2881 gggtggggat taataatcag atacaaaaag ggccgccagc tcataacgcc tccggaaggt
     2941 gcgaacaagt ccctgatatg agatcatgtt tgtcatctgg agccatagaa cagggttcat
     3001 catgagtcat caacttacct tcgccgacag tgaattcagc agtaagcgcc gtcagaccag
     3061 aaaagagatt ttcttgtccc gcatggagca gattctgcca tggcaaaaca tggtggaagt
     3121 catcgagccg ttttacccca aggctggtaa tggccggcga ccttatccgc tggaaaccat
     3181 gctacgcatt cactgcatgc agcattggta caacctgagc gatggcgcga tggaagatgc
     3241 tctgtacgaa atcgcctcca tgcgtctgtt tgcccggtta tccctggata gcgccttgcc
     3301 ggaccgcacc accatcatga atttccgcca cctgctggag cagcatcaac tggcccgcca
     3361 attgttcaag accatcagtc gctggctggc cgaagcaggc gtcatgatga ctcaaggcac
     3421 cttggtcgat gccaccatca ttgaggcacc cagctcgacc aagaacaaag agcagcaacg
     3481 cgatccggag atgcatcaga ccaagaaagg caatcagtgg cactttggca tgaaggccca
     3541 cattggtgtc gatgccaaga gtggcctgac ccacagccta gtcaccaccg cggccaacga
     3601 gcatgacctc aatcagctgg gtaatctgct gcatggagag gagcaatttg tctcagccga
     3661 tgccggctac caaggggcgc cacagcgcga ggagctggcc gaggtggatg tggactggct
     3721 gatcgccgag cgccccggca aggtaagaac cttgaaacag catccacgca agaacaaaac
     3781 ggccatcaac atcgaataca tgaaagccag catccgggcc aaggtggagc acccatttcg
     3841 catcatcaag cgacagttcg gcttcgtgaa agccagatac aaggggttgc tgaaaaacga
     3901 taaccaactg gcgatgttat tcacgctggc caacctgttt cgggcggacc aaatgatacg
     3961 tcagtgggag agatctcact aaaaactggg gataacgcct taaatggcga agaaacggtc
     4021 taaataggct gattcaaggc atttacggga gaaaaaatcg gctcaaacat gaagaaatga
     4081 aatgactgag tcagccgaga agaatttccc cgcttattcg caccttccct agaacaacaa
     4141 tgcctcgatt ggtttaaaga gctaggttac caataccaaa atggctatga catcgcacca
     4201 gatggtgata tgcctgaacg tgaaaactat cagcaagtca cgctacaggc gcgtttagtc
     4261 tctgccttgg aaaccttaaa cccaaatgtg cccagtgaca ccttaatcga agtagcgaaa
     4321 atcgttgcca caccgcaaac gcctgtactg attaaaaaca acaaactctt tcaccaatat
     4381 ttaatcgaag gtgtgcctgt tgaatactcg gtaatggagc aagaacaatg ggtgaccaag
     4441 catactcatg ttcgattaat tgattttgtg ttgtggtcaa ctaaaactgg ccaccgcgtt
     4501 agagtttttc cagtatcggt tttctgattc gtttggtggt aacccaccat tatattcgtg
     4561 cggtcttagt gcgctgtaat atccaacgat atagtccgtt atggcgtgag ctgcatcgct
     4621 gaaacttaca tagcccgtcg ctggcaccca ttcgttcttc agactcctga agaagcgctc
     4681 cattgggctg ttatcccagc agtttccacg ccgactcata ctctgcctga tccggtatcg
     4741 ccacagtaac tgccggaact gcctgctcgt ataatgactg ccttgatcgc tgtggaacat
     4801 caccccgacg ggcttaccac gggtttccca tgccatttcc agtgctttca tggtaagcct
     4861 gctgtccggc gagaacgaca tggcccagcc tgacctgccc ctctttcctg cgcaaccacg
     4921 ctaccagcga gtcaattcca ccaatacggc gaaacagttc atcgctgtta aaaaacggct
     4981 gcaacgcctc atttgttgcc atagtttgct cggaaacaac gaggccgtcg tccatgtgct
     5041 cgattaactc acgcggcacc ggctccataa taaatttacg gccagcctcc accagctttc
     5101 tgacttcctg atccactttg aatgtggcga cgccaagctc tttttgtaca aagggagtaa
     5161 ttacggcttt cacatcacac ctttcatcac tgattgggct ttatctgctg cccggcattc
     5221 tctgtttaag cacaaccatt tcctgacggc ataacacagc aatagcggtc ctggcaccaa
     5281 tttgcttacc agccaggtat tgctttacct cgcggcgact cacgccatca agaagcatct
     5341 ttaacgcttc acgggacaat ttgttgtatt tgcgtgccat taatctactc cgcagaacca
     5401 tacaatctac gtaacgtgtc ggcgacagaa gatacagata tctctccggt cgcagcccct
     5461 acggtaaggt ctgccagttc aggtgaatca aatacctgca ccccgttacg gcgtagaaat
     5521 aacagcgcac tgtttagcgc ggtacgctta ttggcatcat tgaatatatg ccctctcgct
     5581 gtagccacca ggtaggtggc ggagacttcg aaaaggtcgg tgatctcttc gtaggcaact
     5641 ctggcctgaa ctctcccgat aatggcctct gccctgcccg gatcagacat tcccggcagg
     5701 ccgccgtagc ggtttatatt cgcatcatga agcgcaataa gttcttccgg tgatatatgc
     5761 ctcattatcg gttaaccagt tccttgttgg tggagtccag ggtgtcaaac agggatgcaa
     5821 attcagcatc cagcgccgct tttttgtagg cttcgaaagt agccttgctg acaattactg
     5881 ctggctcacg gcctctgcgg gtgatttcaa cctcttcccc ggcctcaaca ttgttgagca
     5941 cttcagaaag gttgccgcgc gcggtacgga agttaatgga ttgcataaac acctcgtgta
     6001 ctcgttatgt gtacacaatt ataaacttca caggcataaa gcaccagcac tttgcggctt
     6061 aacaaacctc taggcaggtc attcgtagcc taatgtccga actgctaaag catccaagtt
     6121 gctgtagaat caccgccaat tacataagcc tgaaataagt ggatgaaaat gacaagtatt
     6181 caacaacgtg cagagcttca tcgtcaaatc tggcaaattg ctaacgatgt caggggttcg
     6241 gtcgatggat gggattttaa gcaatacgtt ctgggcgcac ttttctaccg ttttatcagc
     6301 gaaaattttt ccagttatat tgaagccggt gatgacagta tctgttatgc gaaactggat
     6361 gacagcgtaa ttactgatga cattaaagac gatgccatca aaaccaaagg ctacttcatc
     6421 tacccaagcc agcttttctg caacgtagct gcgaaagcaa ataccaatga cagattgaat
     6481 gcagatttaa acagcatctt cgttgctatc gaaagttctg cttacggtta tccttcagaa
     6541 gctgacatca aaggtttgtt tgctgatttc gataccacca gtaaccgcct gggtaacacc
     6601 gttaaagata aaaatgcccg cctggctgcg gttctgaaag gggttgaagg gttaaaactt
     6661 ggtgacttca acgaacatca gattgacctg ttcggtgatg cctatgagtt cctgatttct
     6721 aactatgcgg caaatgccgg taagtcaggc ggtgagttct ttacaccgca gcacgtctcc
     6781 aagctgattg cacaactggc tatgcacggg cagaccagcg ttaacaa
//
LOCUS       DAEBXD010000158         6763 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEBXD010000158 DAEBXD010000000
VERSION     DAEBXD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.158"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            64..2496
                     /locus_tag="KJL94_003744"
     CDS             64..2496
                     /locus_tag="KJL94_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBD4411924.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2563..4032
                     /locus_tag="KJL94_003745"
     CDS             2563..4032
                     /locus_tag="KJL94_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBD4411925.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4032..5693
                     /locus_tag="KJL94_003746"
     CDS             4032..5693
                     /locus_tag="KJL94_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBD4411926.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5914..6255
                     /gene="symE"
                     /locus_tag="KJL94_003747"
     CDS             5914..6255
                     /gene="symE"
                     /locus_tag="KJL94_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBD4411927.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6302..6622)
                     /locus_tag="KJL94_003748"
                     /pseudo
     CDS             complement(6302..6622)
                     /locus_tag="KJL94_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2001 a         1612 c         1556 g         1594 t
ORIGIN      
        1 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaaccc
       61 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      121 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      181 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      241 gctgacatcg tgctgtacca caagccaggc attcccttag ccgtgattga agcaaaagcc
      301 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      361 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      421 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
      481 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
      541 gactattacg acgatggcag cggcaaatcg cctcgttatt atcaacttca ggcaatcaac
      601 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
      661 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
      721 aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa aaataatgat
      781 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
      841 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
      901 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
      961 gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc cacccaaatt
     1021 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1081 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc tccttataaa
     1141 gttgtccgtg ttgatattga tgtcgacctg caaggctggc gcccaaccaa agggcaaact
     1201 gacttaaacg gcgaagtgat tgacgatcgt atctataacc agaaagattt cgatcgcacg
     1261 atggtgatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgc
     1321 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1381 cgccgcgccc tggttaatct caacccgcag caggtgaaaa agaacgacaa atacgtcatg
     1441 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     1501 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccactggtgt ggatgctaaa
     1561 acctgcaaac tggtagtact agaccagaac atccagtcga tgaccaaatt caagcaaatt
     1621 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     1681 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     1741 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     1801 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     1861 taccaggtta ccgataccga tgatgtcggc ccatttccgg aagaagacga gaagaaaatc
     1921 cgcaagtttc acgtcaacgg tgtagcggtg ggcgttattg cacagcgcgt tcagtattac
     1981 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2041 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2101 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2161 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2221 acccgcaaag agcgcgccga gaacgtgcac aagcggaact acttcacaaa atactctgaa
     2281 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2341 atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca gcatgggcac cttaccggag
     2401 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     2461 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     2521 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     2581 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggacggcga tgcgcagcgt
     2641 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     2701 gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg gcgcagttgg
     2761 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     2821 tttccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     2881 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgccaggtg
     2941 atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3001 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3061 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3121 atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca tgtaaagaac
     3181 aaatacgtga agagcgtcgc cgatcatcag acactgcaac aacagatcca cggtgttgag
     3241 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctgcacgg cattgaagta
     3301 ccggtacaaa ttcgtcacga caacaccctg aataaaccgc tttcctcctg ggatgagcaa
     3361 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3421 aactttccgg cagagatgca aacccgcgaa acggcggatc tgtttctgca actgattgtg
     3481 gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc cggatggcac actatttggc
     3541 gaaggtgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     3601 gtgcgtttac cgaatggtgt gtttaatccc tataccggca ttaaaaccaa cctgttgttc
     3661 tttaccaaag gtcagccaac caaagagatt tggttctatg aacatccgta tccggcgggc
     3721 gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc ggagatcgac
     3781 tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca agcgtggaaa
     3841 gtcagcattg atgacgtgat tgcgcgcaac ttcaatctgg atattaaaaa cccacatcag
     3901 gcggaaaccg tcagccatga tccggacgaa ctgttagcgc agtatgcaaa acagcaggcg
     3961 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4021 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc tggacctcgg
     4081 cgttgcaaac ccgttccacc gccgggcgcg gcagctccgg taagattgat ttgtatggta
     4141 ttaagaaatt gcgtgagctg attctggaac tggcggtgcg cggtaaactg gtgccgcagg
     4201 atccgaatga tgaaccggcg tcggacctgc tgaagcgtat tgcggcggaa aaagcagagc
     4261 tggtgaagca aggcaaaatt aaaaagcaaa aaccactgcc agaaattagt gaggaagaga
     4321 agccgtttga attgccggag ggatgggagt gggtgcacct ccctgatatt tattgctcaa
     4381 tttctgagtc atctagaaaa ataaaatcct ccgaaatact acctgaggga aaatatcctg
     4441 ttatcgagca aagccaagaa tttatttctg gttattgcaa caatgaatgt ctgttaataa
     4501 agttaaataa tccagttata gtttttggtg atcacacaag gaatattaaa tttattgatt
     4561 ttgattttgt tgtcggcgct gacggtgtga agattttatc acctatatta atttgtgagc
     4621 gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac gcgagacatt
     4681 ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa gagcgaatag
     4741 ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa tccctgacca
     4801 gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca gacagccaaa
     4861 acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac accctattta
     4921 ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc gtaatgggca
     4981 aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa cgtattgcgc
     5041 aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca ctgccgccaa
     5101 ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt cgtattggta
     5161 atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat ttatatcaag
     5221 tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga acagtgaaag
     5281 aatcaggagt caaagggcca aatagtcgct tttataaagg acaaattgtt tattctaaaa
     5341 tccgcccgtc attatccaaa gtttttttag cagagtataa tggtctttgc agtgctgata
     5401 tgtatccatt agactgttac attaatccaa attacttatt gaaatatatc ctttccattc
     5461 catttcttat gcaagtcaaa aaagcagaga acagaataaa aatgccaaaa cttaattctg
     5521 acagttttta caatattatt gttgcgattc ccccctataa tgaacaacaa gcaatatttg
     5581 ataagataaa ttctattgaa gcagtatgca atggtcttat ttcttatatt gggatttatc
     5641 ataaaaccca actgcatctt gcagacgcac tcactgacgc agcgataaac taattccctc
     5701 gccctcacaa aaccaataaa gcggctaaat agccgcttta ttcacatcac caaaatttat
     5761 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     5821 ctgatgatgt atccaggtat ttagcacggt gcggatgtgc gccaacacac tcgcaccgct
     5881 aatcacaatc actatttata gagaatagta atcatgactg acacgcattc tattgcacaa
     5941 ccgttagaag cagaagtctc cccggcaaat aatcgtcaat taaccgtgag ttatgcgagt
     6001 cgttacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6061 gccggttttg ccaccggcac ggcggtagat gtcaaagtga tggaaggctg tattgtcctc
     6121 accgcccaac ccgccgccgc cgaagagagc gaactgatgc agtcactacg caaggtgtgt
     6181 aagctgtcgg cgcgtaaaca aaggcaggta caggaattta ttggggtaat tacaggtaaa
     6241 cagaaagtcg cctgatacat tgacccggta gcgccagtgc taccgggtat tattcgccgc
     6301 gttataccaa cgcgctctcc atctgttttt caaatgcctg acgaattaat cccataatgt
     6361 aaggaagttg tgcgaggtcg ctgaaaccaa tttctacatc gccattaccc cagcgcccaa
     6421 catttgtcac atctttggca atacctttcg gatcgaccag ttcatgaaac tgcatattaa
     6481 gcgacaatcg cagtcggctt ttttgcggca caacatcaac aaaattcgtt tccgctttaa
     6541 acgcaatata tagcttcaat acttcctgcg tgatccctgc gtccaggcgc ataacttcat
     6601 cgcgaagatg atcgaataac actgaggtag cctgagttta acggacactc cttcctgaaa
     6661 tagaatggca tcagaaggag ctaataatga gcagaaaaac ccaacgttac tctaaagagt
     6721 tcaaagccga agctgtcaga acggttcttg aaaatcaact ttc
//
LOCUS       DAEBXD010000159         6733 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEBXD010000159 DAEBXD010000000
VERSION     DAEBXD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.159"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..306)
                     /locus_tag="KJL94_003749"
     CDS             complement(<1..306)
                     /locus_tag="KJL94_003749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411928.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     "
     gene            complement(392..1273)
                     /gene="narH"
                     /locus_tag="KJL94_003750"
                     /pseudo
     CDS             complement(392..1273)
                     /gene="narH"
                     /locus_tag="KJL94_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1270..5010)
                     /gene="narZ"
                     /locus_tag="KJL94_003751"
                     /pseudo
     CDS             complement(1270..5010)
                     /gene="narZ"
                     /locus_tag="KJL94_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5092..6480)
                     /gene="narU"
                     /locus_tag="KJL94_003752"
     CDS             complement(5092..6480)
                     /gene="narU"
                     /locus_tag="KJL94_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBD4411929.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVTVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1601 a         1768 c         1776 g         1588 t
ORIGIN      
        1 accgccggcg ctgataccgg caagaatggt cgcaaattcc cgctccgtgc agcgggcgat
       61 ttccggatat ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc
      121 atccacaaag aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc
      181 aaagcgcaga acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac
      241 atagcgggga agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg
      301 ggccatataa ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac
      361 catgacaagc cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc
      421 tcgacgcttc ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac
      481 ccacgcaggt ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga
      541 tacatttttc tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc
      601 taatgcacaa acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt
      661 cgcgtttgta gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt
      721 cgcacaggcg cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt
      781 gcatggcctc gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt
      841 cccagtttgg gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc
      901 gagcagtagg gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga
      961 aaggttcgta gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta
     1021 ttacgcccat cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc
     1081 cgccttgcca ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt
     1141 cgacgttgtt aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg
     1201 tcaccgaaca ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg
     1261 aacgtatttt cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt
     1321 aacgttcttc atcttgcgga tcatgatgaa ttcgtcgcgg ttcgagccga cggtgccgta
     1381 gtagttaaag ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca
     1441 aacgcgggtg accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat
     1501 attcataatg cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct
     1561 gaccaccgcg cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc
     1621 gacaatggtc agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga
     1681 gagcgtcagc attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg
     1741 cgtcaggaag ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact
     1801 gacgctacgg gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat
     1861 ccacggatga tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc
     1921 atgaacgttg gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt
     1981 ggggctggag ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc
     2041 ctctttgtgc agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca
     2101 cgctttgact gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc
     2161 gtcaatggcg gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt
     2221 gagtttgccg aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt
     2281 gccaagtttg tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc
     2341 gcgctccacc gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt
     2401 gcgccagtcg agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg
     2461 ttgtaatacc acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt
     2521 ggcaatacct ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc
     2581 agaaagcgga tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca
     2641 ggtggcggtg ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag
     2701 cgtcaccagc aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg
     2761 tttgatcccg tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag
     2821 atacttctgc atatactcgt ggcctttgcc ggaggagcca agcaggttag agcgccagac
     2881 aaacaaatta cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc
     2941 gcccgatttc agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc
     3001 agctttaatc ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg
     3061 ttcggcgcga acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg
     3121 tgaaagtaac tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa
     3181 aaacgaggtg ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg
     3241 caaccagccg gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc
     3301 gctttgcccg acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc
     3361 catgtgatac cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt
     3421 atgggcggta tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt
     3481 aatttgctca ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt
     3541 ttcatcttcc agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac
     3601 caccggacag gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag
     3661 caccggattg tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa
     3721 ggccacgcct gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt
     3781 accggcggca atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga
     3841 accgttcggc actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt
     3901 gttgctttcg cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta
     3961 gctaccatcg tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca
     4021 gtagttgata aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc
     4081 cattgccatc gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa
     4141 tttggccact tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac
     4201 ttcggtaaag aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat
     4261 gatgtagctg gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt
     4321 catcggcgag gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc
     4381 aagcagcgac agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg
     4441 cgagaaaccg gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc
     4501 aatcagctgg tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg
     4561 tttgtagctc aggcactttt gcggatcgtt cataatcgat gcccacgcca gtaccggatc
     4621 gctgtgttgt ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta
     4681 tttcaggcgg ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg
     4741 aggttcatga ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca
     4801 ggtcaccaga ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc
     4861 gtgggtggaa cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc
     4921 gcggttgcta tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa
     4981 gtagcgaaag cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg
     5041 gatctgatgc cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg
     5101 gctgaacttc cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat
     5161 taaaaacact ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc
     5221 ctgcggaata aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc
     5281 cgtttcggtg acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg
     5341 ataaatggtt atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag
     5401 acccgcggtc agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc
     5461 tgtgcccggt aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat
     5521 caacgtcacc cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat
     5581 ggcaccaata aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt
     5641 ttttgccagc atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta
     5701 aagcaggctc agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga
     5761 ggcgcgtgaa ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag
     5821 tagcggtacc caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg
     5881 tacgccattg acgccgagaa aggcaaatac agttacaaaa atgaccagcg gtgcaaccag
     5941 ctgcattaca cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg
     6001 tttggctttt ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc
     6061 aaaaccgcat agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac
     6121 ggcaattccg agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg
     6181 gcgtccaccg aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac
     6241 ggagggtaat gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt
     6301 gagattaacg gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact
     6361 gactgatatc cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg
     6421 attttctggt ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat
     6481 attgttcctc atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa
     6541 tagccagtta ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag
     6601 aaagcgtaac cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta
     6661 actaacaagc taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct
     6721 tcagatcgtg aat
//
LOCUS       DAEBXD010000160         6635 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEBXD010000160 DAEBXD010000000
VERSION     DAEBXD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.160"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            154..504
                     /locus_tag="KJL94_003753"
     CDS             154..504
                     /locus_tag="KJL94_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBD4411930.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="KJL94_003754"
     CDS             557..952
                     /locus_tag="KJL94_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBD4411931.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="KJL94_003755"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="KJL94_003755"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBD4411932.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="KJL94_003756"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="KJL94_003756"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBD4411933.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KJL94_003757"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KJL94_003757"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBD4411934.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="KJL94_003758"
     CDS             complement(5323..6423)
                     /locus_tag="KJL94_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBD4411935.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1534 a         1727 c         1562 g         1812 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaa
//
LOCUS       DAEBXD010000161         6615 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEBXD010000161 DAEBXD010000000
VERSION     DAEBXD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.161"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(97..2100)
                     /locus_tag="KJL94_003759"
     CDS             complement(97..2100)
                     /locus_tag="KJL94_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411936.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2097..3233)
                     /locus_tag="KJL94_003760"
     CDS             complement(2097..3233)
                     /locus_tag="KJL94_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD4411937.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3226..5505)
                     /locus_tag="KJL94_003761"
     CDS             complement(3226..5505)
                     /locus_tag="KJL94_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411938.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5516..6604)
                     /locus_tag="KJL94_003762"
     CDS             complement(5516..6604)
                     /locus_tag="KJL94_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBD4411939.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1512 a         1823 c         1777 g         1503 t
ORIGIN      
        1 actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc
       61 ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg
      121 gaataattgt tcgcaaagca aaacaccgtt attcattgct tctacccgtg cctcgctttc
      181 tgtattacga aattgtgcca acacatgtgc cagccgataa aaacccaccg cggtgaggtc
      241 attcgccagc aactctgcct gattaatagc gctctgttcc tgatagcgcc agccgttatg
      301 gagcagttga ataagtaacg cctggcagcg catcagcaac tgatgagcag tagacggcac
      361 aggcagaacg ctggcagaag atagtgatac caccacaggc gcagtttctg cgtccagcgc
      421 ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa
      481 actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat
      541 gcgttcaatg gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc
      601 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
      661 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
      721 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
      781 cggcgcgctc aacatttgcc aggcatcagg cgacagcggt acaacgctgc ttaagcggtt
      841 gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
      901 cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtc
      961 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1021 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1081 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1141 ccacggcaca ggaggaactt caccaacact gtcgctccgg gccatctctt gtgcgcaatt
     1201 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1261 gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccatcg
     1321 ccagctgcag cgttccgctg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag
     1381 cggctggctg atgccgctca gccataatgc ctggcttagt tgttgaacat attgacgaca
     1441 cgttttgcct tcttcgctgg caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat
     1501 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     1561 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     1621 actgctgcgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcacc
     1681 cggcgcacag aacaactcaa tggtgatgct tttagcgacc agcgcctgtg cgcgtttgcg
     1741 ggtggcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc attcctcttc
     1801 ttttcccgta ggctgggcgg tagcacaaag tcgttgataa cttaacacca gcatcacgcg
     1861 atgacggcac atgccgctgg ccccataggt gcactgagcc tctttcagtg cctggccgtt
     1921 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgctgt tctcatggct
     1981 gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt
     2041 gcttaacgcc gtcagtgcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2101 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gtgtcatggc ggctatttgt
     2161 gcgccgacat taatcagcgc ctgggccgta tcgcggtcat agcaaggtgt tgctgtgcta
     2221 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacttggtgc
     2281 gtcagcaatg atgatgaacc cccttcataa aaatcgctca cgaggataat gacgcttttt
     2341 gcaggttgtt caataagttg ccgaccatac tccatggcac tggcgatatt ggttccgccg
     2401 cctaattgca ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2461 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca taaacaggcc
     2521 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     2581 accagttgcc attgctcgct ttggcgttta atgcggctgt taaagcgggg gaattcgata
     2641 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     2701 tcaaagtttc gcgccagtgg aataaatgag cggcgacgac gatcgcggac accagaaaaa
     2761 gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt ccacaacctg gcgcacaatc
     2821 ctgcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt
     2881 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     2941 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3001 gtgtgaatac tgttgatcca ctcaggagtg gtgagatttg agccacctaa tccactagag
     3061 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtgatct
     3121 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3181 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3241 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3301 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3361 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3421 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3481 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     3541 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     3601 ttcggcacta gcgttgggat gttccagacg catcagcgca ccaagagtcg cgccgcgatc
     3661 cagtgccagg acatgaatcg cctgactgtg tcgctccagc aaggctaccg ccgcagaaag
     3721 cgaaacgccg gggagttgca caccgctatg tagatcgcgc aggatatgac acaacgcttg
     3781 ccagctatgc aagtgagcgt gaaactcctt ttcatccggt ctgccgttgg attcacacag
     3841 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     3901 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     3961 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4021 ggacgccaga cccgctgacg ccgcttggct aagacatgtg gtaatgctgc cgattccacc
     4081 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4141 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4201 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4261 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4321 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcaag tgacatcttt
     4381 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4441 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4501 cggcaacggc gcattcaggg cttctttaat cagcgatcct gctatggcat ccagccagtc
     4561 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagt gccatcgcat gcagatgagc
     4621 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg tgagaaccgt
     4681 ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     4741 cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     4801 ggtgagataa caacctgtaa cggcatctgc cagcgacggc aattctggcg tgttaattac
     4861 ctgcggacat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacaac
     4921 taacacgtcg ccattatttt gctgcatcgc ccatccaatc cagcgggcca taaaggcttc
     4981 gcgctgacga tttagagcat cgccggggga gtcgccccgc agttgggcaa aatagtgcgc
     5041 cagtgcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt
     5101 gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc tggctttcct cttgcgtatc
     5161 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5221 tgcctgaata cgacgatcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5281 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5341 taccggaagc tggtgggcta aaaacagttc gtctacccgg tcattaaaat cagcagggcc
     5401 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5461 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg
     5521 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     5581 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     5641 cggcaatgca gtccaccagg tcggctggct cacctgcgcg attcgctaaa aaccaggcgc
     5701 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg
     5761 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta
     5821 actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     5881 aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa
     5941 aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg
     6001 cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt
     6061 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg
     6121 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc aggtaaagcg
     6181 gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc
     6241 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6301 cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6361 ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac
     6421 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6481 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     6541 ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg
     6601 acatatattc atccc
//
LOCUS       DAEBXD010000162         6402 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEBXD010000162 DAEBXD010000000
VERSION     DAEBXD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.162"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KJL94_003763"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KJL94_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBD4411940.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="KJL94_003764"
     CDS             complement(1433..1759)
                     /locus_tag="KJL94_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBD4411941.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="KJL94_003765"
     CDS             1894..2235
                     /locus_tag="KJL94_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBD4411942.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="KJL94_003766"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="KJL94_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="KJL94_003767"
     CDS             complement(2833..3543)
                     /locus_tag="KJL94_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBD4411943.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="KJL94_003768"
     CDS             3651..3956
                     /locus_tag="KJL94_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBD4411944.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="KJL94_003769"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="KJL94_003769"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="KJL94_003770"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="KJL94_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1628 c         1500 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tg
//
LOCUS       DAEBXD010000163         6376 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEBXD010000163 DAEBXD010000000
VERSION     DAEBXD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.163"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            60..1386
                     /gene="pgaC"
                     /locus_tag="KJL94_003771"
                     /pseudo
     CDS             60..1386
                     /gene="pgaC"
                     /locus_tag="KJL94_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1388..1801
                     /gene="pgaD"
                     /locus_tag="KJL94_003772"
                     /pseudo
     CDS             1388..1801
                     /gene="pgaD"
                     /locus_tag="KJL94_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1851..2639)
                     /gene="phoH"
                     /locus_tag="KJL94_003773"
     CDS             complement(1851..2639)
                     /gene="phoH"
                     /locus_tag="KJL94_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBD4411945.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3247..4512)
                     /gene="efeB"
                     /locus_tag="KJL94_003774"
     CDS             complement(3247..4512)
                     /gene="efeB"
                     /locus_tag="KJL94_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBD4411946.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4518..5645)
                     /gene="efeO"
                     /locus_tag="KJL94_003775"
     CDS             complement(4518..5645)
                     /gene="efeO"
                     /locus_tag="KJL94_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBD4411947.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5703..>6376)
                     /locus_tag="KJL94_003776"
     CDS             complement(5703..>6376)
                     /locus_tag="KJL94_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBD4411948.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1533 a         1681 c         1534 g         1628 t
ORIGIN      
        1 taaccaacct gaaatagacc ttattcgtcc tgagttttca acagcctggt atccgaaaaa
       61 tgattaatcg catcgtatcg ttttttatat tatgtctggt gttatgcata cccctatgcg
      121 tagcgtactt tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta
      181 tgtccattat gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc
      241 cgtggggaga aaacgcacca gctccccagt tgaaagataa tccgtctatc tccattatca
      301 ttccctgttt taatgaggag aaaaacgttg aggaaaccat acacgccgct ttagcacagc
      361 gttatgagaa cattgaagtt attgccgtaa atgacggttc aacagataaa acccgtgcca
      421 tcctggatcg catggctgca caaattcccc atttgcgggt cattcatctg gcgcaaaaac
      481 aggggaaagc cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt
      541 gcattgatgg cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt
      601 tgtacaaccc gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc
      661 tggtgggtaa aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc
      721 agcgtatcta tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg
      781 ccctggcaga agtgggttac tggaatgacg atatgatcac cgaagatatt gatattagct
      841 ggaagctgca gttgaatcag tggccgattt ttttacgagc cacgggcgct gtgctggata
      901 ttaatgcctg aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc tcagggcggt
      961 gcagaagtat tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt tcgaatgtgg
     1021 ccgctgtttt ttgaatactg cctgacgaca atatgggcct tcacctgcct ggtcggtttc
     1081 attatttacg cagtccaact tgccggtgta ccgttaaata ttgaattgac acatatcgct
     1141 gcgacacata ctgccggaat attattgtgt acgttatgtt tactgcaatt tattgtcagc
     1201 ctgatgatcg agaatcgcta tgagcataat ctgacttcat cgcttttctg gattatttgg
     1261 ttcccggtta ttttctggat gctgagcctg gcaacgacat tggtatcatt tacacgagtc
     1321 atgttgatgc ctaaaaagca acgcgcccgt tgggtaagtc ctgatcgcgg gattctgaga
     1381 ggttaatatg aacaatttaa ttattacgac ccgacaatca ccagtacgtt tactggttga
     1441 ttatgttgcc acaaccatct tgtagacatt atttgctttg ttcatattct tattcgccat
     1501 ggatctgctg acgggttatt actggcaaag cgaggccaga agccgacttc agttctattt
     1561 tttgctggca gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca ataagctgcg
     1621 ttttcaaaaa cagcagcatc atgcagccta ccaatatacg ccgcaagaat atgcagagag
     1681 cttagcaata cctgatgagc tctatcagca actacaaaaa agccacagga tgagcgtaca
     1741 cttcaccagc caggggcaaa taaaaatggt tgtttcagaa aaagcgctag tccgggcata
     1801 aacacccaaa acaaagcccg gttcgcccgg gctctgcacc gataatacac ttaactgtat
     1861 gcatgcagcg tacgttggca aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg
     1921 atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg
     1981 ccgcgaggca aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg
     2041 aggcgggtta aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg
     2101 acaactgcat tttcaaaggt acgtccacgc atataggcga acggcgcggt ttccaccttc
     2161 cctatttccg gtcgcaggca gtactgcata aaggaagccc ctaagcgccg gaccagcaag
     2221 tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga tatcaccagg taagaagcca
     2281 agatcttcat cggcttgcag aactggacgg gtgacgataa tcctgtcgac atccttatgt
     2341 atcagggcct ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc
     2401 gtggcgaata tcagctgctt actctcaata gcctgcagat agtgcaattg cgcttcattt
     2461 cgcgcaagga tgggcgaggt atcgcgactg tcgcgggcca taccaatggc ttctacgccg
     2521 ctcatctgca caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc
     2581 cgtctcagca cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg
     2641 gagagcacct tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcgca
     2701 cgaacatcag agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa
     2761 aaacggctac gcacaccgtt tgcggcgtcg gtaacacgag taaagaaagg aagtgagaaa
     2821 aatacagagt gacgaagttg ctaccggagg agaaatctcc gcgagtggtg gagcgttgct
     2881 taacaaaaca ttgtccagaa caggacgtta ccatccgcga tctccatagt gactgactat
     2941 cactgccggg aacttccgct gctacttaat aagtacaaca gaactcgcat tcattgcaac
     3001 aatatattca cttgtattta acgataaaca cccatttcgg tgacattaat ttctactgga
     3061 aagatgacag agtgatgaca gtgatggaaa aagctgtgtg ctttccgcag gattcgcagg
     3121 gctggtaaaa tgtgcctcgt gtttgccgga ttcggcgcga acgccttacc cgacctacaa
     3181 gtgattaata atttgtgcac aaaactcatg cccgataaac ctgcgcatcg ggcatatttg
     3241 cgtcgttcaa acctgcaata gcgcccttcc cagataatcg ttcgcgtcct tcactcccgg
     3301 cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt
     3361 gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag
     3421 caaccccata tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg
     3481 cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc
     3541 cagcgcgatc accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg
     3601 cattcccagc ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag
     3661 cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc
     3721 accgattgtc cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa
     3781 cttatcattc tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg
     3841 cgtctctttg cctttactac gcgccgcgtg atcggaaata aacccttccc gcttccagcg
     3901 cacactgagc aaatccggcg tgtgtttgat gatatcgcgc agcgcatggt tcaccgtgtc
     3961 ctgggtgttg gcgcaaatct gtagcaacac atcaccatga cataacgccg catccagcga
     4021 gtcgttgggg aaacgcgtca tcttctgcag cttttttggc atctgtggcg caaggccaaa
     4081 gcgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat
     4141 gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg tttctggtgc
     4201 tgctccgccc tgactcagaa aagcaaaacg ctgagtcaac aaacgaaaca accgctcaag
     4261 atcggcttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg
     4321 cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg
     4381 tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc
     4441 cagcgcacca atccctttca gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc
     4501 tttatactgc ataacgctta atccagtccc agcacaccgc gaagttgcgc cagatcttcc
     4561 gccagcgcag taatcggccc tttcagcgca ttccggtcgg catcggtcaa tttgtcgtag
     4621 gtttcaaaac cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg
     4681 gcatcgactt ttgccaacag ttccgggttg gctttttgca gttgtggacg cagcagatcg
     4741 acaattttct gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag
     4801 cgatcttctt caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcgccg
     4861 ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca
     4921 tcggtataaa gctgctcagc gtactgatcc atccctttgg tggtgttgtc gccaaacaat
     4981 gctttttcca gacggtggaa accagtgaat tttgggtcgg cggctttttg ctcgtaatca
     5041 tcttcacggg cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaata
     5101 cgctcatagt gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct
     5161 ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca
     5221 tacgctttat atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca
     5281 tccgccgttg cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag
     5341 gtcatatcgt attcgccagg ctgtaaattc gccgtcattt tctggctaaa accaggggcg
     5401 atattttccc gctcttccac caccatcacg cctttgagga tctcccactc cagcgccttc
     5461 tggctatggt tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt
     5521 tcgcactgct tatccgtcac ggtcactttg acctgcggca catcagcggc gttagccata
     5581 aaagcagaag aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg
     5641 gtcatgacga gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt
     5701 tgttacgcgg agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc
     5761 gccgggatga gataaataaa ccagacggcg acttcgctga cgctcggtgc ttcctgatag
     5821 ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag ttgagaggac cgcgctcata
     5881 tcgaaggcga tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca
     5941 ccagccgcca gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca
     6001 ccaagattga ggcgaatacc gccccagtag agcaggaagc cgagcaccac ggcagtagca
     6061 agaccgagca ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc
     6121 aggaaaaaga ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc
     6181 gcccagccat gatgatttcc acgctgcaat gcgctatcga ctgcctgttc cagttgcact
     6241 ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg
     6301 atcaccgcca cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt
     6361 tcgttaatga agatcc
//
LOCUS       DAEBXD010000164         6356 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEBXD010000164 DAEBXD010000000
VERSION     DAEBXD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.164"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..370
                     /locus_tag="KJL94_003777"
     CDS             <1..370
                     /locus_tag="KJL94_003777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411949.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KJL94_003778"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KJL94_003778"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBD4411950.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KJL94_003779"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KJL94_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBD4411951.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KJL94_003780"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KJL94_003780"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBD4411952.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KJL94_003781"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KJL94_003781"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBD4411953.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KJL94_003782"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KJL94_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBD4411954.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..>6356)
                     /locus_tag="KJL94_003783"
     CDS             complement(5095..>6356)
                     /locus_tag="KJL94_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBD4411955.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1440 a         1837 c         1525 g         1554 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaggaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagtta
//
LOCUS       DAEBXD010000165         6333 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEBXD010000165 DAEBXD010000000
VERSION     DAEBXD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.165"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            7..438
                     /locus_tag="KJL94_003784"
     CDS             7..438
                     /locus_tag="KJL94_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBD4411956.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="KJL94_003785"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="KJL94_003785"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBD4411957.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="KJL94_003786"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="KJL94_003786"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBD4411958.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="KJL94_003787"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="KJL94_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBD4411959.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="KJL94_003788"
     CDS             complement(4440..5147)
                     /locus_tag="KJL94_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBD4411960.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="KJL94_003789"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="KJL94_003789"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBD4411961.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="KJL94_003790"
     CDS             complement(6005..6208)
                     /locus_tag="KJL94_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4411962.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1406 a         1811 c         1648 g         1468 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccaccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg ctt
//
LOCUS       DAEBXD010000166         6284 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEBXD010000166 DAEBXD010000000
VERSION     DAEBXD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.166"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            6..485
                     /gene="eutP"
                     /locus_tag="KJL94_003791"
     CDS             6..485
                     /gene="eutP"
                     /locus_tag="KJL94_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBD4411963.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            460..1833
                     /gene="eutA"
                     /locus_tag="KJL94_003792"
     CDS             460..1833
                     /gene="eutA"
                     /locus_tag="KJL94_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBD4411964.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            2352..3046
                     /locus_tag="KJL94_003793"
     CDS             join(2352..2598,2598..3046)
                     /locus_tag="KJL94_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4411965.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(3165..3974)
                     /gene="murI"
                     /locus_tag="KJL94_003794"
     CDS             complement(3165..3974)
                     /gene="murI"
                     /locus_tag="KJL94_003794"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD4411966.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(4008..5468)
                     /locus_tag="KJL94_003795"
     CDS             complement(4008..5468)
                     /locus_tag="KJL94_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4411967.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(5461..5739)
                     /locus_tag="KJL94_003796"
     CDS             complement(5461..5739)
                     /locus_tag="KJL94_003796"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411968.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            complement(5720..6205)
                     /locus_tag="KJL94_003797"
     CDS             complement(5720..6205)
                     /locus_tag="KJL94_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4411969.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
BASE COUNT         1833 a         1391 c         1391 g         1669 t
ORIGIN      
        1 cattcatgaa acgaattgcg tttgtgggta gcgtcggggc aggaaaaaca acgttattta
       61 atgcgttgca ggggaattat accctcgcca gaaaaacact ggccgtggaa tttaatgata
      121 agggcgatat tgatacgccg ggtgaatatt ttagccatcc ccgctggtat cacgccttaa
      181 ttaccacgct acaggatgta gacatgctga tctatgtcca tggcgcaaat gacccggaaa
      241 gtcggttgcc tgccgggttg ctggatattg gcgtcagcaa acggcaaatc gccgtcatca
      301 gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg aaagttattg cttgagacgg
      361 gtttcgaaga gccgattttc gagctaaaca gccacgaccc gcaaagcgtg cagcaactgg
      421 tggattatct ggcatctctc accaaacagg aggaagcagg tgaaaaaact catcacagcg
      481 aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa tgtccgtggt tctgcgcgcc
      541 agcattatta ccccggaagc ccgtgaagtg gcggatctac tgggttttac catcaccgaa
      601 tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca gcgtgccagc cgacaaaacc
      661 gaaagccagc gcatccgcga aaccatcatt gcccagctgc cggaaggcca gtttaccgaa
      721 agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa aacagtcgct gggcgatttt
      781 gtcgtcgcca gcgccgggcc gcacctcgaa tccgtgatcg ccggtcacgg agccggggcg
      841 caaacccttt ctgaacaacg gctgtgtcgg gtactgaata tcgatatcgg cggtggcacc
      901 gcgaactacg ccctgttcga tgccggaaaa atcagcggca ctgcctgtct caacgtcggt
      961 ggtcgcctgc tggaaaccga cagccagggg cgcgtggttt acgctcataa accggggcag
     1021 atgattgtgg atgagtgctt cggtgcaggc actgacgccc gttcgctgac cggcgcgcag
     1081 ctggtgcagg ttacccggcg gatggcggcg ctgattgtcg aagtgattga cggaacgctt
     1141 tcgccgctcg cgcaggcatt gatgcaaacc ggtttgctgc ccgcaggtgt tacgcccgaa
     1201 atcattacgc tttctggagg cgtgggcgaa tgttatcgcc accagcccgc caacccgttc
     1261 tgttttgccg atattggccc acttctggca acggcgctgc atgaccatcc gcgcctgcgt
     1321 gagatgaatg tgcagtttcc ggcgcaaacc gtacgctcca cggtgattgg cgcgggtgca
     1381 catacccttt cgctctctgg cagcacaatc tggctggagg gcgtacaact gccgctgcgc
     1441 aatttgccgg tggcgatccc gattgatgaa acggatctgg tgagtgcctg gcaacaggcg
     1501 ctgcttcagc tggatcttga tcccaaaact gacgtgtacg tgctggcgct tcccgcctcg
     1561 ctgcctgtgc gttacgccgc ggttctgacg gtcatcaacg cgctggtcga tttcgtcgcg
     1621 cgttttccga atccgcatcc cctgctggtg gtggccgggc aggactttgg taaagctctg
     1681 ggcatgttgt tgcgcccaca gctacaacaa ctcccgttgg cagtcattga cgaagtgatt
     1741 gtccgcgcgg gggactatat cgacattggt acgcctcttt ttggcggatc ggttgtgccg
     1801 gtgacggtga aatcactcgc atttccttcc tgaatacggt agttatcagg tatggtgctt
     1861 ggtgtctggg tgttagtggg attatatccg gcagtgtatc cggttaaggt gattggtctg
     1921 gcaagatgat tggatgcttt tgtggtgaaa aatgttttgg tgttttagca taaccctgaa
     1981 gaaagaataa aaataaaaag gaaaaaacca aaaccagaac agaaccggaa agagaagaaa
     2041 agagaaaatg aaaaataaaa accaaaaaag aaagaatact taaatcttcg catgttcttc
     2101 gggtggtacg caagtttgtt agtctgaccg cacaataaat tttcgcttag aaaataataa
     2161 gttacataat attctgcttt tttagataaa tataacccga aaaaatcatt gttaatctta
     2221 tgcatttatt atttgattgt aagttgtgat tattataatt tagttattgt tagtatgttt
     2281 tataatgctg gatgggtgat gcctctaatt agttgaatct gatgtataat acgggctttt
     2341 gaggttatct catgaccagc gttaatattc attgtccccg ttgtcagtca gctcaggttt
     2401 accgccatgg tcagaaccct aaaggccgtg acagacttcg ctgccgtgac tgccaccgtg
     2461 tgtttcagtt cacttatact tatcaagcac gtaagccggg tatgaaagag ctgataactg
     2521 aaatggcctt taatggtgcc ggggttcgcg ataccgccag gacactgaaa attggtagta
     2581 acaccgtcat ccggacttta aaaaactcgc gccaaagcga ataacttctt cgcctgttgc
     2641 ccatgctgat gtggcgttta tctgcgagct tgatgagcaa tggagctacg ttggcagtaa
     2701 agcccggcaa cattggctcg ggtacgcgta caacaccaaa acaggcggtg tactggccta
     2761 cacttttggt ccccgaaccg atcaaacgtg ccgggagcta ctggcactgc ttacaccctt
     2821 caacatcggc atgctgacca gcgatgactg gggcagctat ggccgggagg tgccgaagaa
     2881 taagcatctg accggcaaaa tattcaccca acgcattgag cgtaataatc tgacgctacg
     2941 cacccgcatt aagcggttgg gtcgtaaaac aatctgcttc tcacgctcag ttgagatcca
     3001 cgaaaaagtg atcggagcgt ttatcgaaaa acacatgttc tactaattgg atgcactacc
     3061 cagtatgtaa ctgggtttgt ccataaaatg caccttactc agttggtgtc caacttttgg
     3121 ggtgcagttc agaaggacgg gattttacgg tgcaccggat aaatttaaaa ctgcctgata
     3181 ctgaatgtat gaggtttcgt aatgctcaat tcccctagta ccgttttgta gatctccata
     3241 cttccactag tatttatttg caaagttcct atatttgagg agtttaataa atttagctgt
     3301 cccaagtgat ttttaataga gatagcctga tcatgtgcag gattaataaa ttttatatcc
     3361 ggtgcaactg cttcaaaaaa attctgaaca attggatagt gagtacagcc gagaataatt
     3421 tcatttactg gatggaggga aattaaatgg ttaacatgtt ttttgatgtc attcaagatt
     3481 gcaggagcat taagattacc ttcctcaatt aacattgcaa gatttttgct tggttctcca
     3541 aatacattta ttgttgaatt caattttttt attagctctt tgtgacaccc cgttttaata
     3601 gtaaactcag tagcgataat accaacattt ttatattgct ggcgaatcac atcttctact
     3661 gcaggttcaa tgatggataa gataggaaac ggaaagctct tagcatatcc gctgtattct
     3721 tcggattcca aagttgagga tatagtatta catgcgacag caactagttt gacattcctt
     3781 gactgtaaaa aagtcagcat ttttttagtc aaactgatga tatcatcggc agaacggttg
     3841 ccataaggtg cattcatatt atctccacaa tagataatat cttcaccagg aagcaaatta
     3901 attatttcct ttaccgatgt caatccacct acacctgagt ctattacacc aattggagat
     3961 tcagcactaa acatatttaa cctcatcttt tgtagcttat ggaaaattta attactagtt
     4021 cttttttccc aagaaaatgg ttcgaaatca gagtttggat ctttccagtc agattttcgg
     4081 aaggcgtaaa ttaatattgg aattaataaa aatagggcaa ctaatactaa caaataaaga
     4141 acatagctgg ttggactacc agttgggatt tgctcgggcg gaataaaact taatacaaaa
     4201 gccagaaaag accctaagaa tccaagtgtt gcccagaaaa ataaagcagg ggctcgatat
     4261 gggcgttttg tatcaggttg agaatgtcgt agatagatag cactagcgaa catgagcata
     4321 tacataacta aatagagcat aactgttaac tgtgataaaa tctgaaaagc tgcttgaact
     4381 gaaggcatta tcacaaaagt aacagaaaga actgagacaa ttataccttg tacaaacatt
     4441 agatggtgcc ccattccatg acgattggtt ttttggaaaa acttaggtaa ataaccagct
     4501 ttcgcgatcg ctaatacacc tgtattaggc cctgcaatcc aagtaaccac cccacccagt
     4561 acgccaattg ctagcataaa tgccatcaca gagccaagcc atggcactcc agcccattcg
     4621 aacagcatat cgtaggcaac caacaaactt tgagttagac taattttatc tgtaggaata
     4681 ataaaggcga cccctaaagt actgagcagg aatattgcaa tagtacctat agaagcgatg
     4741 gtaattgcaa ttgggtattt tttagttgga ttatctaaat cgttaacatg gaccgcattc
     4801 atttccatcc ctgcataagc aagaaatatg ctggcggcaa gaacaatatt ctggaagtta
     4861 gctatattag ggaaaagatc accccaacct aatgggatca ccgctggttt accgctgaac
     4921 cacagatagg agaaaccaag aataacaagg ataatagctg gaataattgt ccctataata
     4981 ccgcaccact tagatacctg cgagaaaata ccaacgccac gtaatgcaac acaaatagct
     5041 ccccagtaaa caaatagtac gaaaaataaa atataccatt tattactggc aattgattct
     5101 gccaccccca tatcaacatc aataaatgca accgagactg caccaaatgt cagtactgtt
     5161 ggaaagtagg ggatgacttg tagccaggtc ataagcattc ctacaaaacc ccagcgagcc
     5221 ccaaatgctt ctcctatcca tcgaaatgcc cccccttttt gtggatatgt tgtagctagc
     5281 tcagccgcaa caaaggcaac aggtactaaa aacataattg cagcaaaggt atagtaaaaa
     5341 atggcaccta agccgtattc tgcttcagaa ggtataaatc gtagacttac tacagcagtt
     5401 atattcatga tagcgagtgt ccccactgtc attttcttac ttttgggtgc gatattagca
     5461 ttagacattt ttatactctc gaacattttt aaaatagaaa catataatta cgatgtaaat
     5521 ataaaaacta atattaactt taacacttat cttaaaaaca ctaacgcaat ctgctttaca
     5581 aaaataagta atacctaaag tcgtaacgac accaatagtg ttttcatcga catgcttcaa
     5641 aacctcttta gcattcatta tcaggtgatg atcatcaagt ggaatttcac acaatttcac
     5701 atcacagtaa cggcagaatt tatgccagca tatttgcaca ggatcgcaaa tcaggttcgg
     5761 ttgatttacc ttgtacttcg cgttttttac accactgcca ttttaacgcc aagcggccga
     5821 gcatagcagc ttcagatgaa ccaatggttg agcaacctaa ggtgtgtcgc gaatcgggga
     5881 aactccacga atcggaaagc atacgcacac agcgaccttc aggttccgcc atttgtggat
     5941 attcatcttt ataaatcatg tttttatcaa tagctaaatt aagcaactca cggatttcat
     6001 catcaagcca tgtttgacag aaagtagcta agttttgtcg tgaatcccca tcaaggatga
     6061 gctcatcacg tatggcgctg aatacatttc gagatttctt ctcatctaca cgcaaatgat
     6121 atttatgcaa tataaatgaa agctcaagtg agctatgaat atcgactgct tctctataac
     6181 aagaattacc tgatttattt ttcataataa acgccactca atacaatcag attaaacatt
     6241 acaaacacaa tctggatttt gcaattttaa aactatcaat ttta
//
LOCUS       DAEBXD010000167         6268 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEBXD010000167 DAEBXD010000000
VERSION     DAEBXD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.167"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..666)
                     /locus_tag="KJL94_003798"
     CDS             complement(<1..666)
                     /locus_tag="KJL94_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBD4411970.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="KJL94_003799"
     CDS             complement(656..2413)
                     /locus_tag="KJL94_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBD4411971.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="KJL94_003800"
     CDS             complement(2429..3313)
                     /locus_tag="KJL94_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD4411972.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="KJL94_003801"
     CDS             complement(3324..3929)
                     /locus_tag="KJL94_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD4411973.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6268)
                     /locus_tag="KJL94_003802"
     CDS             complement(4100..>6268)
                     /locus_tag="KJL94_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBD4411974.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1835 a         1568 c         1316 g         1549 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgttt
//
LOCUS       DAEBXD010000168         6252 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEBXD010000168 DAEBXD010000000
VERSION     DAEBXD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.168"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="KJL94_003803"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="KJL94_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBD4411975.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="KJL94_003804"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="KJL94_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBD4411976.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KJL94_003805"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KJL94_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="KJL94_003806"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="KJL94_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1656 a         1456 c         1476 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag at
//
LOCUS       DAEBXD010000169         6252 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEBXD010000169 DAEBXD010000000
VERSION     DAEBXD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.169"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KJL94_003807"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KJL94_003807"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBD4411977.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KJL94_003808"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KJL94_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBD4411978.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KJL94_003809"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KJL94_003809"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBD4411979.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="KJL94_003810"
     CDS             4130..4885
                     /locus_tag="KJL94_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBD4411980.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="KJL94_003811"
     tRNA            4964..5037
                     /locus_tag="KJL94_003811"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6252
                     /locus_tag="KJL94_003812"
     CDS             5152..>6252
                     /locus_tag="KJL94_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4411981.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLN"
BASE COUNT         1554 a         1569 c         1619 g         1510 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca at
//
LOCUS       DAEBXD010000170         6250 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEBXD010000170 DAEBXD010000000
VERSION     DAEBXD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.170"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="KJL94_003813"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="KJL94_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBD4411982.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="KJL94_003814"
     CDS             578..1027
                     /locus_tag="KJL94_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4411983.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="KJL94_003815"
     CDS             complement(1028..1261)
                     /locus_tag="KJL94_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBD4411984.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KJL94_003816"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KJL94_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBD4411985.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="KJL94_003817"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="KJL94_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBD4411986.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="KJL94_003818"
     CDS             complement(2212..3021)
                     /locus_tag="KJL94_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBD4411987.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KJL94_003819"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KJL94_003819"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBD4411988.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="KJL94_003820"
     CDS             complement(4677..5471)
                     /locus_tag="KJL94_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4411989.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="KJL94_003821"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="KJL94_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            5836..6081
                     /locus_tag="KJL94_003822"
                     /pseudo
     CDS             5836..6081
                     /locus_tag="KJL94_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1637 a         1573 c         1419 g         1621 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cggggctgaa tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg
     6061 ctgaggtata tgccggcctg acacaggcta agtgtgcccg ggaatcggtg caagtataaa
     6121 aaacagcctg ttgcggtata agaatcgacc atgcagagag agaaaacacc ggagtggagg
     6181 gaaaagcaga aaagctcacg tggcataagg cgtggacagc ggtatcgtct ggtttttcaa
     6241 tttcctatac
//
LOCUS       DAEBXD010000171         6239 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEBXD010000171 DAEBXD010000000
VERSION     DAEBXD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.171"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            393..2051
                     /locus_tag="KJL94_003823"
     CDS             393..2051
                     /locus_tag="KJL94_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBD4411990.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="KJL94_003824"
     CDS             complement(2048..3004)
                     /locus_tag="KJL94_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBD4411991.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="KJL94_003825"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="KJL94_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBD4411992.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="KJL94_003826"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="KJL94_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBD4411993.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1469 a         1481 c         1736 g         1553 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgat
//
LOCUS       DAEBXD010000172         6212 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEBXD010000172 DAEBXD010000000
VERSION     DAEBXD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.172"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..378
                     /locus_tag="KJL94_003827"
     CDS             <1..378
                     /locus_tag="KJL94_003827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4411994.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KJL94_003828"
     CDS             complement(454..918)
                     /locus_tag="KJL94_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD4411995.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KJL94_003829"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KJL94_003829"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBD4411996.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KJL94_003830"
     CDS             complement(1787..2476)
                     /locus_tag="KJL94_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBD4411997.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KJL94_003831"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KJL94_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBD4411998.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KJL94_003832"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KJL94_003832"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBD4411999.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KJL94_003833"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KJL94_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBD4412000.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1575 a         1688 c         1547 g         1402 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg ac
//
LOCUS       DAEBXD010000173         6059 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEBXD010000173 DAEBXD010000000
VERSION     DAEBXD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.173"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            382..1410
                     /gene="torT"
                     /locus_tag="KJL94_003834"
     CDS             382..1410
                     /gene="torT"
                     /locus_tag="KJL94_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBD4412001.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1383..2075)
                     /gene="torR"
                     /locus_tag="KJL94_003835"
     CDS             complement(1383..2075)
                     /gene="torR"
                     /locus_tag="KJL94_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBD4412002.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2162..3334
                     /gene="torC"
                     /locus_tag="KJL94_003836"
     CDS             2162..3334
                     /gene="torC"
                     /locus_tag="KJL94_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBD4412003.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3334..5874
                     /gene="torA"
                     /locus_tag="KJL94_003837"
     CDS             3334..5874
                     /gene="torA"
                     /locus_tag="KJL94_003837"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBD4412004.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5871..>6059
                     /gene="torD"
                     /locus_tag="KJL94_003838"
     CDS             5871..>6059
                     /gene="torD"
                     /locus_tag="KJL94_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD4412005.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1680 g         1392 t
ORIGIN      
        1 tccatttcgg tcccagttag gacgctttct catcaagcca gtccgcccac cactgcatca
       61 tctccctacg cttatcgaga tactgagcat ggttgtaaat tcaattaacc cagtgcgttg
      121 acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct aatgctctgt tacaggtcac
      181 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg tcgcgttttt
      241 cagtctatta tttaatcatt aagtaatcta atgctatgat ttttaaatta tttatgtgat
      301 tttgtcatcc tttaggtgaa aaaggttgag tcgcaaagtg gaatgcatct agcataaagc
      361 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
      421 ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca tttcacggtg
      481 caagcctcta tgccgcttaa agccaaacgc gtatggaaac tgtgcgcgct ttatcccagc
      541 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
      601 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
      661 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
      721 tcatttcccg acctgcaaaa gcaggtagca aatctgccgg tgatcgaact ggtaaatgct
      781 cttgatgatc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
      841 cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct gctgatgccc
      901 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
      961 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgc
     1021 aacctgttgc aggagatgct ggaacgccac ccagaaatcg acgtcgttgc cggaacggcc
     1081 attgcggcag aggcggcaat gggtgaaggg cgtaacctga aaacgccgct taccgtggtg
     1141 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
     1201 gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat caggcaatta
     1261 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
     1321 gccgaccgag aacatatccg ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt
     1381 tatcagcaca catcagcggc taagaaataa ccttcaccat gttgcgtcac cagtaaatcc
     1441 gcgctgagtt tatgacgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
     1501 tccacccgac gcgcagaaag catacgcagc agacgctcac ggctgagaat ttcgcccgga
     1561 ttagtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcgcca
     1621 tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg atagcagtag
     1681 tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag atttttcacc
     1741 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
     1801 agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt
     1861 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggtaa gttgatatcc
     1921 agcagaatta aatctaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
     1981 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc
     2041 ggcttatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
     2101 tatctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta caggggttat
     2161 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
     2221 ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
     2281 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
     2341 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
     2401 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
     2461 ttaccagacc tttattgccc actccattga tacacctgaa aaattcgaag ccaaacgcgc
     2521 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgtcgctc
     2581 ctgccataac tacgatgcga tggatcatgc caggcagcat cctgaagcgg cacgtcagat
     2641 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
     2701 gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
     2761 tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg cgaaaggcga
     2821 taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
     2881 cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc agcgtgtact
     2941 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
     3001 ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
     3061 ggccaccgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggccta
     3121 tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatcgccca
     3181 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagtctcga
     3241 taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
     3301 taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
     3361 tcacgtcggc gttttctggc acaacttggc ggcttaaccg tcgccgggat gctggggccg
     3421 tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc tgtcatctcg
     3481 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgatggt gaaggatggt
     3541 cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
     3601 ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg cgtggactgg
     3661 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
     3721 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
     3781 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
     3841 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
     3901 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcaat ggaagtgtat
     3961 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
     4021 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
     4081 tattacgagc agctaaaagc gaaagtcgcc gccggtgaag ttgaggtcat cagcatcgat
     4141 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
     4201 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcac atacgctgta cagtgaaaac
     4261 ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt cctgccgtat
     4321 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
     4381 attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt
     4441 attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg
     4501 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ctggcactac
     4561 aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc cggctctacg
     4621 tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac tattccgatt
     4681 gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa cggtaaatcg
     4741 gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt ccatcgccat
     4801 cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat cgccatagat
     4861 aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt
     4921 gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat cgccatgaaa
     4981 caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg cgagctgtgc
     5041 cgtcgcttta atcgtgaaga agcctttacc gaagggctgg acgaaatggg ctggctgaaa
     5101 cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct gccagcgttt
     5161 gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat gtttgttcgc
     5221 caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag tggcctgatt
     5281 gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg gcatccgatg
     5341 tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta tccgttgcat
     5401 ctgcaatctg tgcatccgga tttccgactt cactcgcagc tatgtgagtc ggaaacgctg
     5461 cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca ggatgccagc
     5521 gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg
     5581 gcaggagcag tagtttctga ccgctatgca cccggcgtgg cgcgaattca cgaaggggca
     5641 tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac
     5701 gtgctgacca tcgacatcgg gacttcgcag ctggcgcagg cggccagtgc gcacactacg
     5761 ctggtggaaa ttgagaagta caatggaaca gtggagcagg tgacggcgtt taacggcccc
     5821 gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc
     5881 tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc tcccgtgaac
     5941 tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg ttttcgttgc
     6001 tgaaaagcga accgccgctc actgcggcgg tgaatgatct ggaaaacagt attgccacg
//
LOCUS       DAEBXD010000174         6054 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEBXD010000174 DAEBXD010000000
VERSION     DAEBXD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.174"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            430..1338
                     /locus_tag="KJL94_003839"
     CDS             430..1338
                     /locus_tag="KJL94_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBD4412006.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="KJL94_003840"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="KJL94_003840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD4412007.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="KJL94_003841"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="KJL94_003841"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBD4412008.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="KJL94_003842"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="KJL94_003842"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBD4412009.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6054)
                     /locus_tag="KJL94_003843"
     CDS             complement(5673..>6054)
                     /locus_tag="KJL94_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412010.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1490 a         1576 c         1562 g         1426 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagtaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgtcaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc gact
//
LOCUS       DAEBXD010000175         6028 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEBXD010000175 DAEBXD010000000
VERSION     DAEBXD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.175"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="KJL94_003844"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="KJL94_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBD4412011.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="KJL94_003845"
     CDS             1207..1869
                     /locus_tag="KJL94_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4412012.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="KJL94_003846"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="KJL94_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBD4412013.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="KJL94_003847"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="KJL94_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="KJL94_003848"
     CDS             2795..3100
                     /locus_tag="KJL94_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBD4412014.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="KJL94_003849"
     CDS             3103..3507
                     /locus_tag="KJL94_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBD4412015.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="KJL94_003850"
     CDS             3497..3796
                     /locus_tag="KJL94_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBD4412016.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="KJL94_003851"
     CDS             3982..4278
                     /locus_tag="KJL94_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD4412017.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="KJL94_003852"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="KJL94_003852"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBD4412018.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="KJL94_003853"
     CDS             5627..5995
                     /locus_tag="KJL94_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD4412019.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1470 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DAEBXD010000176         5906 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEBXD010000176 DAEBXD010000000
VERSION     DAEBXD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.177"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(97..573)
                     /locus_tag="KJL94_003854"
     CDS             complement(97..573)
                     /locus_tag="KJL94_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBD4412020.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(632..1921)
                     /gene="bioA"
                     /locus_tag="KJL94_003855"
     CDS             complement(632..1921)
                     /gene="bioA"
                     /locus_tag="KJL94_003855"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBD4412021.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2008..3048
                     /gene="bioB"
                     /locus_tag="KJL94_003856"
     CDS             2008..3048
                     /gene="bioB"
                     /locus_tag="KJL94_003856"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBD4412022.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            3045..4199
                     /gene="bioF"
                     /locus_tag="KJL94_003857"
     CDS             3045..4199
                     /gene="bioF"
                     /locus_tag="KJL94_003857"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBD4412023.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4186..4941
                     /gene="bioC"
                     /locus_tag="KJL94_003858"
     CDS             4186..4941
                     /gene="bioC"
                     /locus_tag="KJL94_003858"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBD4412024.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            4934..5611
                     /gene="bioD"
                     /locus_tag="KJL94_003859"
     CDS             4934..5611
                     /gene="bioD"
                     /locus_tag="KJL94_003859"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD4412025.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1393 a         1573 c         1662 g         1278 t
ORIGIN      
        1 tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag
       61 tgataccaga tggcattgcg ccatctggca gagtgattaa ctaaacatcg cggtaatcga
      121 tgcgcttgcc agagagtgga aatgaacgtt aaacccgacc atcgcgccgc tggctccttc
      181 atcgacatca atacgctcta catccagcgc gtgaacggta aaaatgtagc gatgggtttc
      241 gcctttcggc ggcgctgcgc catcgtatcc ggttttacca aaatcggtac gcgtctgcaa
      301 aacgccgtct ggcattgcta ccagaccaga gccaaaccct tgcggtaata cgcgggtatc
      361 agcgggtaaa ttaacaacta cccagtgcca ccagccggag ccggttggcg catccgggtc
      421 gtagcaggtg acaacaaaac ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg
      481 tgaaatatta tcgccatcgt aacccatgcc gttaaagaca tgacgatgcg gcaacttatc
      541 gccatcgcgc agatcgttac tgatgagttt catgaaccct cctttcttgt ttgcagaaag
      601 tgtagccaga aaccctcacg ctgacttccc gttattggca aaaaaatgtt tcatcctgta
      661 ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg cgggagaata atatagggcg
      721 gcatcaggta aatcagtttg ccaaaaggcc ggatccatac cccctgttcg acaaagaatt
      781 tttgcagcgc cgccatattc accggacgag tggtttcgac cacaccgatt gcccccagta
      841 cgcgcacatc ggcaaccatt tcggcatcac gggctggtgc cagttgctcg cgcagctgca
      901 cttcaatagc cgccacctgc tgctgccatt cgccggattc gataatcgct aggctggcgt
      961 ttgctgccgc gcaggccagc ggattgccca taaaagttgg cccatgcata aagcagccgg
     1021 cttcgccgtt actgatggtt tctgcaacct cgcgcgtggt aagtgtggcg gaaagggtca
     1081 ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc cggcgcgatt tctgcatatt
     1141 cacaggcaaa cagtttgccg gtacgaccaa atccggtggc gatctcgtcg gcaatcagca
     1201 agataccttc gcgatcgcac attttgcgga ttcgttttaa ccattccgga tggtacatgc
     1261 gcatcccgcc tgcgccctgg acaatcggct caatgatcac cgctgcgatt tcatgacgat
     1321 gcgccgccat caggcgggca aaaccaacca tatcgcgttc atcccattca ccatccatgc
     1381 ggctttgcgg ggcgggagca aacaagtttt ctggcagata gcctttccac aggctgtgca
     1441 ttgagttgtc cggatcgcac accgacatcg cgccaaaggt atcgccatga taaccattgc
     1501 ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcttg ccagtactgc aacgccattt
     1561 tcatcgccac ttccaccgct acggaaccgg agtccgcgag aaaaacgcac tccagcggtt
     1621 gcggcgtcat tgccaccagt ttgcggcata gctcgatggc gggcgcatgg gtgataccgc
     1681 caaacatcac atgcgacatg gcatcaattt gcgtcttcat cgccgcatta agctgcgggt
     1741 gattgtagcc gtggatcgcc gcccaccagg acgacatacc gtcaaccagg cgtctgccgt
     1801 cagacaaaat tagctcgcaa ccttcggcgc tcgccaccgg ataaaccggc agaggggagg
     1861 tcatggatgt gtatgggtgc cagatatggc gttggtcaaa ggcaagatcg tccgttgtca
     1921 taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac aagcctacac cgaattaaca
     1981 acaaaaaaca cgttttggag aagccccatg gctcaccgcc cacgctggac attgtcgcaa
     2041 gtcacagaat tatttgaaaa accgttgctg gatctgctgt ttgaagcgca gcaggtgcat
     2101 cgccagcatt tcgaacctcg tcaggtgcag gtcagcacgt tgctgtcgat taagaccgga
     2161 gcttgtccgg aagattgcaa atactgcccg caaagctcgc gctacaaaac cgggctggaa
     2221 gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca
     2281 ggatcgacgc gcttctgtat gggcgcggca tggaagaatc cccacgaacg cgatatgccg
     2341 tacctggaac aaatggtgca gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg
     2401 ggcacgttga gtgaatctca ggcgcagcgc ctcgcgaacg ccgggctgga ttactacaac
     2461 cacaacctgg acacctcgcc ggagttttac ggcaatatca tcaccacacg cacttatcag
     2521 gaacgcctcg atacgctgga aaaagtgcgc gatgccggga tcaaagtctg ctcaggtggc
     2581 attgtgggct taggcgaaac ggtaaaagat cgcgccggat tattgctgca actggcaaac
     2641 ctgccgacgc cgccggaaag cgtaccaatc aacatgctgg tgaaggtgaa aggcacgccg
     2701 cttgccgata acgatgacgt tgatgccttt gattttattc gcaccattgc cgtagcgcgg
     2761 atcatgatgc cgacctctta cgtgcgcctt tctgccggac gcgagcagat gaacgaacag
     2821 actcaggcga tgtgctttat ggcaggcgca aactcgattt tctacggttg caaactgctg
     2881 accacgccga atccggaaga agataaagac ctgcaactgt tccgcaaact ggggctaaat
     2941 ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac agcaacgtct tggacaggcg
     3001 ctgatgaccc cggacaccga cgaatattac aacgcggcag cattatgagc tggcaggaga
     3061 aaatcaacgc ggcgctcgat gcgcggcgtg ctgccgatgc cctgcgtcgc cgttatccgg
     3121 tggcgcaagg agccggacgc tggctggtgg cggatgatcg ccagtatctg aacttttcca
     3181 gtaacgatta tttaggttta agccatcatc cgcaaattat ccgtgcctgg aagcaggggg
     3241 cggagcaatt tggcatcggt agcggcggct ccggtcacgt cagcggttat agcgtggtgc
     3301 atcaggcgct ggaagaagaa ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta
     3361 tctctggttt tgccgcgaat caggcagtca ttgccgcgat gatggcgaaa gaggaccgta
     3421 ttgttgccga ccggcttagc catgcctcat tgctggaggc tgccagttta agcccgtcgc
     3481 agcttcgccg ttttactcat aacgatgtcg ctcatctggc gcgactgctt gcttccccct
     3541 gtccggggca gcaactggtg gtgacagaag gcgtgttcag catggacggc gatagcgcgc
     3601 cactggcgga aatccagcag gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg
     3661 cccacggcac gggcgttatc ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa
     3721 aaccagaatt gctggtagtg acttttggca aaggatttgg cgtcagtggg gctgcggtgc
     3781 tttgctcaag tacggtggcg gattatctgc tgcaattcgc ccgccacctt atctacagca
     3841 ccagtatgcc gcccgctcag gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg
     3901 atgaggggga tgcacggcgc gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag
     3961 tacaggattt gccgtttacg ctggctgatt catgcagcgc catccagcca ttgatcgtcg
     4021 gtgataacag ccgtgcgtta caactggcag aaaaactgcg ccagcaaggc tgctgggtca
     4081 cggcgattcg cccgccaacc gtacccgctg gtactgcgcg actgcgctta acgctaaccg
     4141 ctgcgcatga aatgcaggat atcgaccgtc tgctggaggt gctgcatggc aacggttaat
     4201 aaacaagcca ttgcagcggc atttggtcgg gcagccgcac actatgagca acatgcagat
     4261 ctacagcgcc agagtgctga cgccttactg gcaatgcttc cacagcgtaa atacacccac
     4321 gtactggacg cgggttgtgg acctggctgg atgagccgcc actggcggga acgtcacgcg
     4381 caggtgacgg cgttagatct ctcgccgcca atgcttgcgc aagcccgcca taaagatgcc
     4441 gcagatcatt atctggcagg agatatcgaa tccctgccgt tagcgactgc gacgttcgat
     4501 cttgcatgga gcaatctcgc ggtgcagtgg tgcggtaatt tatccacggc actccgcgag
     4561 ctgtatcggg tggtgcgccc cggaggcgtg gtcgcgttta ccacgctggt gcagggatcg
     4621 ttacccgaac tgcatcaggc gcggcaggcg gtggacgagc gtccgcatgc taatcgcttt
     4681 ttaccgccag atgaaatcga agagtcgctg aacggcgtgc gttatcaaca tcatattcag
     4741 ctcatcacac tatggtttga tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt
     4801 gctacgcatc ttcatgaagg gcgcgacccg cgaatattaa cgcgttcgca gttgcagcga
     4861 ttgcaactgg cctggccgca acaacagggg cgatatcctc tgacgtatca tctttttttg
     4921 ggagtgattg ctcgtgagta aacgttattt tgtcaccgga acggataccg aagtggggaa
     4981 aaccgtcgcc agttgtgcac ttttacaagc cgcgaaggca gtaggttatc ggacggcagg
     5041 ttataaaccg gtcgcctctg gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc
     5101 gctggcgtta cagcacaaca gcagcctgca gctggattac gcaacggtaa atccttacac
     5161 cttcgcagaa cccacctcgc cgcacatcat cagcgcccaa gagggcaggc cgatagaatc
     5221 attggtaatg agcgccggat tacgcgcgct tgaacaacag gctgactggg tgttagtgga
     5281 aggtgctggc ggctggttta cgccgctttc tgacactttc acttttgcag attgggtaac
     5341 acaggaacaa ctgccggtga tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc
     5401 gatgttgact gcgcaggcaa tacaacacgc cggactgact ctggcgggtt gggtggcgaa
     5461 cgatgttacg cctccgggaa aacgtcacgc tgaatatatg accacgctca cccgcatgat
     5521 tcccgcgccg ctgctgggag agatcccctg gcttgcagaa aatccagaaa atgcggcaac
     5581 cggaaagtac ataaaccttg ccttgttgta gccattctgt atttggttaa attgcgagcg
     5641 agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa
     5701 aattttactt ccgcctcatg cggcgaatgt gggaattgcc caggcggcgg gggatagggg
     5761 ctggagacag ttatccacta ttcctgtgga taaccatgtg tattagagtt agaaaacacg
     5821 aggcaagcga gagaatacgc ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa
     5881 tatcttttat tttttaactg gttaga
//
LOCUS       DAEBXD010000177         5868 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEBXD010000177 DAEBXD010000000
VERSION     DAEBXD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.178"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            11..670
                     /locus_tag="KJL94_003860"
     CDS             11..670
                     /locus_tag="KJL94_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBD4412026.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(871..1248)
                     /locus_tag="KJL94_003861"
     CDS             complement(871..1248)
                     /locus_tag="KJL94_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4412027.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1315..4281)
                     /locus_tag="KJL94_003862"
     CDS             complement(1315..4281)
                     /locus_tag="KJL94_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBD4412028.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4284..4844)
                     /locus_tag="KJL94_003863"
     CDS             complement(4284..4844)
                     /locus_tag="KJL94_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4412029.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(4970..5320)
                     /locus_tag="KJL94_003864"
     CDS             complement(4970..5320)
                     /locus_tag="KJL94_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBD4412030.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5523..>5868)
                     /gene="intI1"
                     /locus_tag="KJL94_003865"
     CDS             complement(5523..>5868)
                     /gene="intI1"
                     /locus_tag="KJL94_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBD4412031.1"
                     /translation="GHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQ
                     AGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRS
                     PLDALPPLTSER"
BASE COUNT         1162 a         1638 c         1783 g         1285 t
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
      721 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
      781 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
      841 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
      901 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
      961 aaaacggtta gcgccggggt tggatttttc agcgttccag ctaaggctaa ggcattcctg
     1021 ttcaagcgca agccgggcta taaagcgcat tatcgcttta cccgtgccct tatttcgatc
     1081 gcactgagaa acatacagct ctttaatatg cagctgaccg ctgtatcggg gcgagggata
     1141 aagaatattg caacatgcca ggccagtaat gttattgccg cagcgcgctc tgatcaccag
     1201 ggtaccggaa agccggttga ataacttttt acagagataa tccttcatca acgcttcctg
     1261 aatgatacct tcgccataat aggggtcgtc tcagaaaacg gaaaataaag cacgctaagc
     1321 cggttgcaga ggccgtagcg gcctgaactt ccccgcgccg atcttggcgc tgctgcgcca
     1381 taggtaatca ccggtcaggt tgatgtgctc ccagccgagt ggcgacaggt actgcaatag
     1441 cgagtcatcg acggcatgac cattgccgcg caacgcatgc gccgcacgct ccaggtagac
     1501 cgtgttccac agcacgatgg ccgccgtcac caggttgagg ccgctggccc ggtagcgctg
     1561 ctgctcgaaa ctgcggtcac ggatttcacc aaggcggttg aagaacacgg cacgggccag
     1621 cgcattgcgc gcctcgccct tgttcagccc ggcatgcacg cggcggcgta gctcgacgct
     1681 ttgcagccag tcgaggatga acagcgtgcg ctcgatgcgg cccaactcgc gcagcgcgac
     1741 ggccaagccg ttctggcgcg ggtagctgcc gagtttcctg agcatcagcg aggccgtcac
     1801 cgtgccctgc ttgatcgagg tggccagccg caggatttcg tcccaatggg cgcggacgtg
     1861 cttgatgttg agcgtgccgc cgatcatcgg cttgagcgcg tcataggcgg catcgccctt
     1921 cgggatgtag agcttggtgt cgcccaggtc gcggatgcgc ggcgcgaagc ggaagcccaa
     1981 gaggtgcatc agggcgaaga cgtgatcggt gaagcccgcc gtgtcggtgt agtgctcctc
     2041 gatccgcagg tcggattcgt ggtacagcag gccgtcgagc acgtaggttg agtcgcgcag
     2101 gccgacattg accaccttgg tgtggaatgg cgcgtattgg tcggagatgt gggtgtagaa
     2161 agtccgtcct gggctgctgc catattttgg gttgatgtgc cccgtgctct ttgccttgct
     2221 agcggttcgg aaattctgtc cgtccgatga tgatgtggtg ccatcgcccc agtgcccggc
     2281 aaagggatgc cgaaactgag cgttgaccag ttcagccaac gctgtcgagt acgtttcgtc
     2341 gcgggtatgc caggcttgca gccaagcgag cttcgcgtag gtcgtgccgg ggcaggactc
     2401 ggccatcttg gtcaggccca ggttgatcgc gtcggccagg atcgtggtca acaacaggtt
     2461 cttgtccttg gccagatcgc ccgatttcaa gtgcgtgaag tgccgggtga agcccgtcca
     2521 ctcatcgact tcgagcagca gttcggtgat cttgacgtgc ggcaggacca tggctgtctg
     2581 gtctatcagc gcctgcgcgg tgtcgggcac cgccgcatcc agcggcgtga tcttcaagcc
     2641 cgactcggtg atgatggcat ccggcaggtc gttggctgcc gccatgcggt tgacggtggc
     2701 aagttgtgct tccagcagcg tcagccgctc atgcagatat tgttcgcagt cggtggccac
     2761 ggccagcggc aattcgctgg actgcttgag gctggtgaac ttctcgggcg gtaccaggta
     2821 gtcctcgaag tccttgaact ggcgtgaacc ctgcacccag atgtcgcccg agcgcaggga
     2881 gttcttcaac tcggacagcg cgcacagttc gtagtagcgc cggtcgatgc cggcgtcggt
     2941 catcaccagt ttctgccagc gcggcttgat gaagccggtc ggtgcatcgg ctggcagctt
     3001 gcgggcgttg tcggtgttca tgccgcgcag cacctcaatg gcatcaagca cgtttttggc
     3061 ggcgggcgcg gcccgcagct tgagcacggc aaggaattcc ggtgcatagc ggcgcagggt
     3121 ggcgtagctc tcgccgatgc gatgcaggaa atcgaagtca tcgggttgcg cgagcttctg
     3181 cgcctcggtg acgctctcgg caaaggaatc ccaggacatg acggcctcga tggcggcaaa
     3241 cgcatcgcgg cctgattgct tggcgtcgat cagcgcctga ccgatgcgcc cgtacagacg
     3301 taccttggcg ttgatggcct tgcctgacgc ctggaactgc tgctgatgct tattcttggc
     3361 agcgttaaac agcttaccca ggatgcggtc gtgcaggtcg atgatttcgt cggtgacggt
     3421 ggccatgccc tcggtggcca gcgccacgag agtggcgtag cgccgttgcg gctcgaattt
     3481 ggccaggtcg gcgggtgtca tctggccgcc ctcgcgggca atcttgagca ggcggttctg
     3541 gtgaaccagc cgctcgatgc cggtaggcag atcgagtgcc tgccatgcct tgaggcgttc
     3601 gatgtgttcc agcatatgcc gcgaatttgg cttggccgga gactggcgca accaagccaa
     3661 ccaggtcgtc ttgccgttgt cccggcgctt gagcagatcg tcgaggcggc ggcgatgcgc
     3721 gtccgccagt ggttcggcca aggcgtcgta gatgcgccgg ttagcacggg tgatcgcctc
     3781 ggcactcgcc cgctcgacgg cgttgagggc gggcagaatg accgactgcc gccgcaggtg
     3841 cccgatcaag gcgctggcca gcacgatgcc tttgtcggtt tgcatcgcca gctcggtcag
     3901 catctggacg gcctgccggt aatggctcat ggtgaagggc cggaaaccga acacggtttg
     3961 cagctcgctc aggtgctcgc gccgggtctg ctcccgctgg ccgtactcgt tccagctttc
     4021 gacgccgacc ttgagctggt cggcgaccag cttcaacaag ggcgggaacg gtagttcatc
     4081 gacgcccagg atgacgccgg gaaagcgcag gtaacagagc tgcaccgcga agcccagccg
     4141 attggctggc ccgcgccgct gtcggatgat cgagaggtcg gtatcgttga atgtgtaatg
     4201 tcggatcagg tcgtccttgg agtccggcaa cgccagcagg ctttcccgct cggcggcgga
     4261 caggatggaa cgacgtggca tatttactga tccgttctca agtattgata cagggtttcg
     4321 cgactgattc cgaattcacg agcaagcttg gtcttttgct cgccagcctc gacacgttgg
     4381 cgcagttcgg caatacgctc agacgacagg gatttcttcc tgccacggta agccccgcgt
     4441 tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg
     4501 gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa
     4561 ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc
     4621 cggcgcaaat catcgagatt gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg
     4681 ccggtgcggg cgaagcttat cagcgcttcc agttgcggac gcttgacatc cttgccggat
     4741 gccttgtcgc taaaagcgcg atcaaccttg acgccttcca gttgccgttc cgggttctgg
     4801 tcgaaggtgc tgaccctgat atacccaatg cgctgtccag tcatggaatt ccctgcaaaa
     4861 tgtcagggaa gactctatga ccttcaacga gatatgtcaa taaattcaaa attcaatcct
     4921 atcctgacgc aatttacaca tggcatctga catcaggtta gggtatgcct caacctgacg
     4981 gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct
     5041 ggaagcgctg atagcactcc agcccgcaga aatgctccac gtactcggcc ccttccggcg
     5101 tgaaggcggc atcgagcggg atttccttgc agcacacgca gcaactggtg ctcggttcat
     5161 tggcgttcat ggtggtgttc ctccattggt tgacgaagcc gacgaaggcc gccgccggca
     5221 tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa
     5281 ggcagtcggg tgtttccaca ccctcggcta ccacctccat tgtcgttttc agaagacggc
     5341 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat ccatcaggca
     5401 acgacgggct gctgccggcc atcagcggac gcagggagga ctttccgcaa ccggccgttc
     5461 gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc gccaggattg acttgcgctg
     5521 ccctacctct cactagtgag gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg
     5581 ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga
     5641 tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg
     5701 agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg
     5761 cgctgaaagg tctggtcata catgtgatgg cgacgcacga caccgctccg tggatcggtc
     5821 gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg aatgcccg
//
LOCUS       DAEBXD010000178         5797 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEBXD010000178 DAEBXD010000000
VERSION     DAEBXD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.179"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="KJL94_003866"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="KJL94_003866"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBD4412032.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..2518
                     /locus_tag="KJL94_003867"
     CDS             1394..2518
                     /locus_tag="KJL94_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBD4412033.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2511..3308
                     /gene="rfaP"
                     /locus_tag="KJL94_003868"
     CDS             2511..3308
                     /gene="rfaP"
                     /locus_tag="KJL94_003868"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBD4412034.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3351..4358
                     /gene="waaO"
                     /locus_tag="KJL94_003869"
                     /gene_synonym="rfaI"
     CDS             3351..4358
                     /gene="waaO"
                     /locus_tag="KJL94_003869"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBD4412035.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            4384..5091
                     /gene="rfaY"
                     /locus_tag="KJL94_003870"
     CDS             4384..5091
                     /gene="rfaY"
                     /locus_tag="KJL94_003870"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBD4412036.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            5116..>5797
                     /locus_tag="KJL94_003871"
     CDS             5116..>5797
                     /locus_tag="KJL94_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBD4412037.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIVLEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLH"
BASE COUNT         1755 a         1065 c         1248 g         1729 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgctgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
     1681 ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gaaaaaggct ttttctatcg
     1741 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
     1801 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
     1861 gactgaagcg gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag
     1921 ccagcagcca gccaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
     1981 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgccgatc gttccattga
     2041 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttgggca
     2101 ggataaaccg cgaaaatttg aggcactggc agaaaaacgc ggcgtgcgca gtaatgttca
     2161 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
     2221 tcctgcctac caggaagcgg cgggaattgt gctgctggaa gcgattactg caggattacc
     2281 ggtactaaca actgccgttt gtggctatgc gcattatatt gtcgacgcta attgcggcga
     2341 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
     2401 gcaatcttca ttgcgccagg cttgggcgga aaatgcgcga cattatgctg atacacaaga
     2461 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
     2521 ttaaagagcc gtttgccacg ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct
     2581 tgcagggtga ggtatttcgt gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg
     2641 gaaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
     2701 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
     2761 tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa ggcatgaatc
     2821 cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc agtctggaag
     2881 attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta
     2941 agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccat cgtgactgtt
     3001 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
     3061 cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt tccaggtcgt tggcgcgata
     3121 aagatcttat agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct
     3181 ggcgctttat gaaggtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
     3241 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
     3301 cgttgtaacc cgtaacttat ttttgccaaa attttggata cagaataaat atgtctcaac
     3361 tcaatgatag tgacatcatc ctttttgagt ataattttca ttatcaaaat ataagatcta
     3421 aaaatactct tgatatagca tttggtattg acagaaattt tctttttgga tgtggtgtag
     3481 ccatcgcatc tattctatta aacaatagag aaatctcttg tgaatttcat gttttcacag
     3541 attatatcag tgataaagac aaattatatt tttctgattt agcaaaacaa tataattcaa
     3601 gaattaatat ttatgttatc aattgtgata agctgaagtc attaccaagc acgaaaaact
     3661 ggacttacgc aacatatttt cgatttataa ttgcagatta tttctatcat aaacatgaaa
     3721 aaatactata tcttgatgca gatattgctt gcaagggtag tattaaagaa ctcttagatt
     3781 atcaattttc tactaatgaa attgccgcag ttgtagctga aagagatatt gaatggtggc
     3841 aaaatcgagc ctcggtatta actacaccac agttagcttc tggatatttc aatgctggtt
     3901 ttttactgat aaatattgat gagtggaatc taaataacat ttcgtcaaaa gctattgaaa
     3961 tgttgcgtga cccagattgg gtaagtaaaa tcacccacct tgatcaagat gtactgaatg
     4021 tattattgaa tggtaaagtg aagtttattt cggagaaata taatacccga tatagtatta
     4081 actatgaatt aaaagacaaa gttgataatc cagtcaatga tgacaccgtg tttatacact
     4141 atgtgggacc tacaaaaccc tggcatgagt gggctgatta tccggtttca cgtagttttt
     4201 tgatcgccaa agcagcttct ccgtggagta aagaagattt acttaaacct gtaaatagca
     4261 atcagtatcg gtattgtgca aaacataaat ttaaacaaaa gcattatatg gcaggcattt
     4321 ttaattattt aaagtattat aaagaaaaat gcttctaaag atttaaggtt aaaataattc
     4381 attatgatat ataataaaac gataaatggg ttgaaagttt tcataaaaga taatgatccc
     4441 ttttatgagc aagttctaaa tgacttttta acttgcaggg ttaaaacatt aaaagttttt
     4501 cgttctattg atgatactaa agtcattctt attgacactg ctcgtggacc tcttgtactt
     4561 aaagtttatg cgccaaagca taaaatgacc gaacgtttct taaaatcttg cataaaaaaa
     4621 gattattatg aaaatttaat ttatcaaacc gatcgggtac gtggtgaagg catccaatca
     4681 attaatgact attttttgct agctgaacgt aaaacattaa atttcgctca ttattatatt
     4741 atgctcattg agtatattga aggtgtcggg ttaaatgagt atttggaaat ttctgaagat
     4801 ctgaaggatc aattatcaga atcgataaaa gagttgcatc agcatggaat ggtgtctggc
     4861 gatcctcata aaggaaattt tatagtatcc gaaaaaggtt taagactaat tgacctttca
     4921 ggtaaaaaaa caaccgctgt tttaaaagca aaagatcgca ttgatcttga acggcactat
     4981 aatataaaaa atgaattgaa agactttggc tatacatatc ttatattcaa aaagaaaatt
     5041 aaaaaagtaa tcagggatgt aaaagtcaaa ctaggattga agagcaaata atgattatga
     5101 tgaagggtaa ttaaattgga ttttaaacat cttactcaat ttaaagatat aattgaactg
     5161 gacaagcgcc ccgttaaact tgatgaacgg gaaacgttta atgtctcatg gggtattgat
     5221 gagaactacc aggttggggc tgcgatttca attgcttcaa ttcttgaaaa gaataaacaa
     5281 aacaaattta cctttcacat aatcgctgat tacttagaca aagagtatat tgaattatta
     5341 tcacaattag caacgaagta tcaaacagta attaaattat atcatattga ttctgagcca
     5401 ttgaaggcgc tacctcaatc aaatatctgg ccagtatcta tttattatcg tttgctttca
     5461 tttgattatt tttctgcgcg attggattca ttattatatc ttgatgctga tatcgtctgt
     5521 aagggttcat tgaacgagtt aatagtatta gagtttaaag atgaatatgg ggcagtggta
     5581 attgatgtag atgctatgca aagtaaaagc gctgagcgtt tgtgtaatga ggattttaac
     5641 ggtagctatt ttaactctgg tgtaatgtat attaatttac gggaatggtt aaaacaaaga
     5701 ctaacggaaa aattctttga tctattatca gatgagtcaa ttataaaaaa attaaagtac
     5761 ccggatcaag atattttaaa cttaatgttt ctacatc
//
LOCUS       DAEBXD010000179         5785 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEBXD010000179 DAEBXD010000000
VERSION     DAEBXD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.180"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..942)
                     /gene="hprS"
                     /locus_tag="KJL94_003872"
     CDS             complement(<1..942)
                     /gene="hprS"
                     /locus_tag="KJL94_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBD4412038.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNK"
     gene            complement(942..1613)
                     /gene="hprR"
                     /locus_tag="KJL94_003873"
     CDS             complement(942..1613)
                     /gene="hprR"
                     /locus_tag="KJL94_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBD4412039.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1746..2159
                     /gene="hiuH"
                     /locus_tag="KJL94_003874"
     CDS             1746..2159
                     /gene="hiuH"
                     /locus_tag="KJL94_003874"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBD4412040.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2268..3272
                     /gene="msrP"
                     /locus_tag="KJL94_003875"
                     /pseudo
     CDS             2268..3272
                     /gene="msrP"
                     /locus_tag="KJL94_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3273..3908
                     /gene="msrQ"
                     /locus_tag="KJL94_003876"
     CDS             3273..3908
                     /gene="msrQ"
                     /locus_tag="KJL94_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBD4412041.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4166..4816
                     /gene="zinT"
                     /locus_tag="KJL94_003877"
     CDS             4166..4816
                     /gene="zinT"
                     /locus_tag="KJL94_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBD4412042.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1306 c         1196 g         1640 t
ORIGIN      
        1 cttattgaga gaaagcgaat ccagtttcac caaaacattg tttttgtctg ccctggcaag
       61 aaacagtata ttctctgtta accgcgaaat attttccagc tcttcaatgt ttccggcaat
      121 tgttttttga tattcagcga tacttctgat ttgactgagc gtaacctgat tctgacccag
      181 taatgcatta attggcgttc taagttcatg agcgagatcg tcagcaaatt gactaaggcg
      241 ctcaaaatct ttgactaaag catgatgcat tttattcaac gcctgcccaa gaggttttag
      301 ttctctcggt aatgcgttaa cctcaaccgg cactcggcta tcgttataat tcagcgcttc
      361 cgttacgcca ctcaactttt tgatctctcg caaccccgtc ctgattaaca acggacttaa
      421 tactgagcac agtataatgg cgacaatgca aattataatg ctattaattt tatactgttc
      481 aagcatatta tgtctggctg aagccaattt agccacagta accgttaatg atggcgaaac
      541 ttcatcaatg ttaattcgta aagcatctat ctctgtatca ttaataatgc ttctgtaaat
      601 gcctgctgcg ctgattgtct cactagcagg tatgttattt aacatgtcat cactgacatt
      661 tgtccggtta acaattttat tgatgccatc actatgaatg atcaaaatat cctgactaac
      721 atccatcatc cggttaaagt acacaggtaa cgtatctgga tttaccccat caattaacaa
      781 ctgcttgatc tgcgctgtcc ggttaatgag tgttgtatca tcgcgccatt tcaactcact
      841 tgccaggcca ttatagagag tccatacaat tccagcgcca gcaacagact gcagaaatat
      901 aaaaagcaag gttaaacgga cggttataga taatcttttc attatttttt taccgctacg
      961 aatgaatagc ccatcccccg gattgtggcg attagctttt caggaaaagg atcatcaact
     1021 tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg tatcactatc aaagttgatt
     1081 ccccaaattt cactcgcaat aaccgttctg ggtataatct cgccagctct ggaggccagt
     1141 agccaaagta actgaaactc cttgcgcgtc agtgtaatac tgatattgtc cctgctgaca
     1201 ctttgactga cagagtccat ttttaagcca ctgatttcta atgttgaatt cagagcgtga
     1261 tgttgcctta attgtgcccg aacccttgct agcaactcag aaaatgaaaa aggttttacc
     1321 agataatcat ttgccccact gtccaaccct ctgactctgt catcgacaga atcccttgca
     1381 gtaaggcaaa taacaggggt ttgctttgcc gttcttaacg tttgtaagat ctgccagcca
     1441 tccatacccg gaagcataat atccagaatg atcaatgcat aatcatcctt cagcgcaaga
     1501 taaagcccat ctctgccatc agaaacggca tcaatgacat aacccgcttc ggaaagcccc
     1561 tgcgttaccc attcctgggt cctttgatta tcttcaataa gtagaatctt catgaaaata
     1621 atatgccata aatcatatgt tatagaaaca acctggttca ttacaaaatt gtaatgctgc
     1681 tgtaaggtta ccctggacgt tttttcgcta tcctcaaaac tcattcacat gacaaggata
     1741 taaacatgtt aaagcgttat ttagtactct cggtagcaac ggcagcattt tcattacctt
     1801 ctttggttta tgccgcacaa caaaacattc ttagcgtgca cattttgaac cagcaaacag
     1861 gaaaacccgc tgccgacgtg acagtcactc ttgaaaagaa agcggataac ggctggttac
     1921 aacttaatac cgccaaaaca gataaggatg gacgaattaa ggcactgtgg cccgagcaaa
     1981 ctgcgactac tggcgattac cgtgtcgtat ttaaaaccgg ggactatttc aagaaacaaa
     2041 atcttgaaag tttcttccct gagattcccg ttgaatttca tattaataaa gtgaacgagc
     2101 attatcatgt gcctttgtta ctcagccaat atgggtattc aacctatcgt ggcagttaat
     2161 gcgtaaatat gtaaaagctg atctctgcta ctgttaggga ggtgactccc tgacagatta
     2221 acagtaaaca gctcttgttg gttaacgaca aaaaagtgtg atggcttatg aaaaagaagc
     2281 aatttttaaa agaaccagat gttacggccg agtcggtatt ctttatgaag cgtcggcagg
     2341 tgttaaaagc actgggcatc agcgcagctg cattttcttt gcctcacgct gcgcatgccg
     2401 atctgcttag ctggtttaaa gggaacgatc gtccgcccgc ccccgccgga aaaccgctgg
     2461 agttcagcaa gcctgccgcc tagcaaaata acctgccact gacgccagca gataaagtct
     2521 ccggttataa caacttctat gaattcgggc tggataaaga cgatcccgcg gctaatgctg
     2581 gtagcctgaa aaccgatcca tggacactga aaatcagcgg cgaagtggca aaaccattga
     2641 ccctcgatca tgatgattta acccgtcgct tcccgctgga agagcgtatt tatcgtatgc
     2701 gctgcgtgga agcgtggtcg atggtggtgc cgtggattgg ttttccgcta cacaaattac
     2761 tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc tttcgaaacg atttatgcgc
     2821 cggaacagat gcctggccag caggaccgct ttatcggcgg cgggctgaaa tatccttatg
     2881 tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac actgatgacc gtaggtgttt
     2941 atggcaaggc gttaccaccg caaaatggcg cgccggtgcg actgattgtg ccgtggaaat
     3001 atggctttaa agggattaaa tcgatcgtca gtattaagct gacccgcgag cgtccgccaa
     3061 ccacctggaa tctggcagcg cctgacgaat acggttttta cgccaacgtt aatccgcatg
     3121 ttgatcaccc gcgctggtca caggctaccg aacgatttat tggttcaggc ggcattctcg
     3181 atgttcagcg ccagccaaca ctactgttta atggttacgc cgaccaggtg gcatcgctgt
     3241 atcgtggcct ggatttgcgg gagaatttct aaatgcgtct gacagcaaaa caagtgatat
     3301 ggctgaaagt ttgcctgcat cttgccggat tgttgccgtt tctctggctg gtctgggcga
     3361 tcaatcacgg tggactgggt gccgatccgg tgaaagatat tcagcatttt accggtcgca
     3421 ctgcactgaa atttttgctt gcggccttgt taatcacacc tctggcacgc tacgcaaaac
     3481 agccgttatt gatacgcact cgccgcctgt taggcttatg gtgctttgcc tgggcgacac
     3541 tgcatttaac cagttacgca ttgctggagt tgggcgtgaa taatctggcg ttattaggta
     3601 aggagttaat tacccggcct tatttaacgt taggaattat cagctgggtt attttgcttg
     3661 ctttagcgtt cacttcaacc cagtcgatgc agcgaaaact cggcaaacac tggcaacagt
     3721 tgcataactt cgtctatctt gtcgcgatcc tggccccaat acattatctg tggtcagtga
     3781 agattatttc accgcagcca ctcatctacg ctgggctggc tgtactgctt ttagccttac
     3841 ggtataagaa gttgctttcc cttttcaacc ggttacgcaa gcaggcccac aacaaattat
     3901 ctctataact tcatattgcc gacaaacttc gcgttatccc ggccggatgc ggcgcgagcg
     3961 ccctatccgg cccacacaga tcgcatcaag ttatgtttcg tttgtcatca acctttcatc
     4021 accagaataa tttattttat ttcaataaga tagttcaata cacctgagaa taccatgctc
     4081 tcatttccta atgagttgtt gcattttttc atatgttaca atataacatt acacataatg
     4141 tacattaact ctggaggaaa ttgttttggc gattcgtctt cataaactgg ctgttgcttt
     4201 aggtgtcttt attgttagcg ctcctgcctt ttcgcatggt catcattctc atggcaaacc
     4261 cttaacagag gtcgaacaaa aagctgctaa tggtgttttt gatgatacca atgtacaaaa
     4321 ccgaacgctc agtgactggg atggagtctg gcaatccgtt taccctttac tgcaaagtgg
     4381 caaacttgat cccgtctttc agaagaaagc ggatgcagat aaaaccaaaa catttgctga
     4441 aattaaagat tattatcgca aaggttatcc gacagatatc gaaatgattg gcattgagga
     4501 cggcattgtc gaattccata gaaataatga aacaacatcc tgtaaatatg attacgatgg
     4561 atacaaaata ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa
     4621 agatcctgaa agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg
     4681 aaaatcatct cattttcaca tttttatggg taatgattcc cagcaatctt tattgaatga
     4741 aatggaaaac tggccaacgt attatcccta tcagttgagt agcgaagaag tggtcgagga
     4801 gatgatgtct cattgatttt tttgcgctga cttcagttat ttaacagaac agtgcctggt
     4861 agtaacaaca ataatttacc aagacaaata agtaagacaa actcagacac gctcatactt
     4921 gagtcgtgtc ttttttatta tgtctgtaga aaaaaactga gaaaaaggct aacaataaat
     4981 acacaactca gccatacaga ctggcagcgc aaatgtttcc aggagcaagt catctgtcat
     5041 ggtaaagcta accagaaata taggttaatt ttggtgttta aacctttttt acttgttagt
     5101 atgatacaaa ggctttcaaa aaagctggga aattcaacaa ctcttggaaa ttatggactt
     5161 tgtcccgcgg ctttaatctg acggccgcgt tccttttttg gctcaattat tgaccccttc
     5221 tctacgcaac ttcagttccc gccagcaact ttgcggcagt ttcataggat caatgtctaa
     5281 aagaataatg gtgaccgata agaaaacgac tgaataactg tagattttcg ctcgaaacct
     5341 tcctgtcagc tccatagcga atcaagcgct gaatgtcaca gtatcgaaca gaaaacagtg
     5401 acgatctaac ccttcaagaa tattctacga ttgttctgtt taggaaaagc aaggcgggaa
     5461 gtcgggagat aagtcattga taaaatggcg gagagagggg gatttgaacc cccggtagag
     5521 ttgcccctac tccggttttc gagaccgatg atacagagtc gaaaaatcaa agagttaaaa
     5581 atttttcttg gaaaaaatgt cttaaaatcg tacacgaaaa atcaatgaat tacataacat
     5641 taaagaagaa tattccaagc cttcttgagt ccaccacctg ttttcaggtg tcattcagac
     5701 gtgatttgcc ccagattggg aaagcacgct tttttctcag cttattccgt tttgcctgtg
     5761 tgttgaataa ttccactgca gttat
//
LOCUS       DAEBXD010000180         5759 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEBXD010000180 DAEBXD010000000
VERSION     DAEBXD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.181"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..402)
                     /locus_tag="KJL94_003878"
     CDS             complement(<1..402)
                     /locus_tag="KJL94_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HBD4412043.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="KJL94_003879"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="KJL94_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBD4412044.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="KJL94_003880"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="KJL94_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBD4412045.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="KJL94_003881"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="KJL94_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBD4412046.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="KJL94_003882"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="KJL94_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBD4412047.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="KJL94_003883"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="KJL94_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBD4412048.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5759)
                     /locus_tag="KJL94_003884"
     CDS             complement(5558..>5759)
                     /locus_tag="KJL94_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBD4412049.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1342 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaaga
//
LOCUS       DAEBXD010000181         5745 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEBXD010000181 DAEBXD010000000
VERSION     DAEBXD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.182"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..173
                     /locus_tag="KJL94_003885"
                     /pseudo
     CDS             1..173
                     /locus_tag="KJL94_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            699..1877
                     /gene="ltrA"
                     /locus_tag="KJL94_003886"
     CDS             699..1877
                     /gene="ltrA"
                     /locus_tag="KJL94_003886"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBD4412050.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFSFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAIQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1960..2139
                     /locus_tag="KJL94_003887"
                     /pseudo
     CDS             1960..2139
                     /locus_tag="KJL94_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2169..2651)
                     /locus_tag="KJL94_003888"
                     /pseudo
     CDS             complement(2169..2651)
                     /locus_tag="KJL94_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2907..3887)
                     /locus_tag="KJL94_003889"
     CDS             complement(2907..3887)
                     /locus_tag="KJL94_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD4412051.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3887..5086)
                     /locus_tag="KJL94_003890"
     CDS             complement(3887..5086)
                     /locus_tag="KJL94_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD4412052.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5408..>5745)
                     /locus_tag="KJL94_003891"
     CDS             complement(5408..>5745)
                     /locus_tag="KJL94_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4412053.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1573 a         1315 c         1369 g         1488 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgta atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgc aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtcg caacgagttt ctcctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1561 cgtcaaatgc agcaatgcgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct
     1621 caacagctga gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga
     1681 tcgagtacta cggaaagttc tggtccagaa atttcaacta tcgactgtgg agtgcaattc
     1741 agtcacgtct gctcaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc
     1801 gaaagctgac gctggtaagg aaggagtatc cgaagctatt tgttcactgg tatttactac
     1861 gtgcatcaaa tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga
     1921 gcagccgggg agtgaaattc tctccggctg actcgactcg cgggttccga cagcgatgac
     1981 gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa tgtggaacca
     2041 gaaaaatggc tgcgttacgt cattgagcat atccaggact ggccggcaaa ccgggtacgt
     2101 gatctgttgc cctggaaagt tgatctgagc tctcagtaaa tatcaatacg gttctgacga
     2161 gccgcttact tttggcgtaa ttctgctgcg cctttactga acacaatgcc gtattcagca
     2221 agaagtccac gaatgtgatt tatcagttgt gtcctttcca ttgaaaccaa ccgacgcata
     2281 ttcaaaaaag tctggacgtc ctgttgctcc agagttttta ttggtacggg tcgtatagtt
     2341 ccatgttggc aggcctcagc tattgcctgt gcgtcgttat aatcattttt ctggcaccgc
     2401 tgataagctt taacatgttg tgccggaagc aatattacat catggtcgag tttcctgatt
     2461 tcccgggccc agtaatgaga tgccccgcca gcctccatcg caacagtcat tttaggctgt
     2521 tgcaccagcc agttaagcag ttgattgcga gaaagtttgg tctttcctgc gggtttgcca
     2581 tgagcattga tactgaaaag ataaaagttc gttttagcca gatcaatgcc ggtgattttc
     2641 tgttcagaca tggtatcccg cttacttttg ttgttaaagt ttatccatca tggcgcaatt
     2701 gaaggctgcc gtgaaagagg gggaagacta tctcatcggt tactgttaaa cggctaagag
     2761 aaactatctt tttcaagcaa agaagatacg gtgaaacagg cgatttcacc gtgaaatact
     2821 gttcaaaaaa aacgctaata aaagcaaaat ttcttttaaa acagccaatt aaaaactggt
     2881 gagttgctgg cgaaaaactt gaattttcac agcgaattca gattatcttt tagtacaaga
     2941 gcaatcatac gatctaactt atcctgaact tccaacggta atctgccgaa ttcatatgag
     3001 aacgtacgtc cctttactcg ttttcgagca aatttatctt tactatcaaa tttccataac
     3061 aaagttacaa ccgctttatc ctttactcct gaatcaagca atgaagacat ttccttggtt
     3121 ataagccgca taatactgtt tttaacctcg tcttcagaaa gattgtcatc agcagtaata
     3181 agctcaatac tcggcgagat attcttcaca acttcatcaa tagatatatc acccagcctc
     3241 agtcgttcac ttaattcagc cagttgcctg taatcggcgt atgtcagctc agactgaaca
     3301 ggaaaaagag acacaaaatc tttaggtacg gacgctgctt ggagggcacg agtcacctta
     3361 gctgccgaca atccctctct ttccgcaatt tgcttctggt tcataccggc ctctttgaga
     3421 cgtactaaac gcaacccaat ttcacgaata ttatgttcaa gtgacgtctg caaatctttc
     3481 gccagatgct gagcttcaga aacagtaagc tcatcatccg ttacaagtac acgtaatcca
     3541 accttgcata gcaaagctgc tgcccgacga cgggaaccat ccaaaatctc aataagatcg
     3601 ccagtccgaa tcccaataca tggatagaat tgttgtaaac gtatagttct ggtgatgtct
     3661 tttagtgagt ccggcgtcag ggctgtctgt tcgcggccat tcacctcttc aaccacaaaa
     3721 gtttgagtat ccactttgtc atgatcaacc ttgatctcag taaattttgc ctttcggcca
     3781 gtattgaggg taaatacatg aactggagcc gatatttctt cagtattatt caggatattt
     3841 gtattcagcg tacggccaat agtcggtcgg tgctttctgt tttccatcag tagttggccc
     3901 ttataaattc aatacgatca aaaactgctt tagcgaaatc ttctgctgcc atacgtgcat
     3961 ttttcaaagc ttcaccactg ccaacataag taacaggatt cgcagatatc acggtatcaa
     4021 acgactcgcc gcttctctca aaaccatcaa gacgtggcat tgaaacatca agcatgtcac
     4081 caccgaaaat ctcttttgtc aggctatggc agtatttatg atctgactta ttcgccagtt
     4141 tcgacatgaa gccaatattt gcctgcaaac ggcatgaaca tccatcctgt tcaataatct
     4201 gtacaagttc aggtaaacga gccaaatatt taagcgtgga atggaaatca acttgtgctg
     4261 gcggcaccgg tgtaaacatg atatctgcgg cagcaatggc atttttcaaa aatgcgtcca
     4321 gatgcgggcc tgtatcaata aggatgaagt cgaaatcatg cttcagctta tcaataatat
     4381 tctcacgcaa tatcgcgtgt ttgttttgtc ctaatagatg ctcctcgcac agagtatccc
     4441 agtttgatgc aataaaggcg tcgtcaatag aagccggcat aacataaacg ccagggataa
     4501 ctgacggaac gataaaatct tccagtaatt cctcacgact cacgttctgc aacatagcct
     4561 gcggtgcggt tgtatcaacc agccccaccg catgcaagta gttcagaaac atagtggctg
     4621 atgactgggg atctaaatca aggagcagaa tacgtaaatc ttctgacagc aggtgaggat
     4681 gagcacgcag ggcgtgagct acggaaacac tactcacggt tttagacaca ccgcctttta
     4741 gactaccgat gaatatggaa tatgcctcac tataacgatc gcgatattta ggaatgccac
     4801 gatgtgcata gatatcgata atgttctgga tggttaatgc atattttgtc gcggttcctg
     4861 ctgggcgttt ttcaaactga tagccctgag cctccatctc accgactgca ccatccacgc
     4921 ttgcgcgcgt taattttgga agcttagcta cagcagactt tgaatagact tgataaaaaa
     4981 catcagcctg aaactcctgc ttttgctcct gaatctgttt tgttagggcg agcaacattt
     5041 tatctgcacg ctgcgcaacc ttacttaact gctcaaaact agtcattccc cctccgtgct
     5101 atgtaggatt ttaacttatt acaaaaaaaa ctgcattagc agggtaatgc atgcaagtac
     5161 atattgtaca tgcttatatt aaaaatgcgc gcattgctga tttattaatc atcggaatga
     5221 atattactgt aagatagtat gcctttccgg gagaaagacg ctgatttccc ccgaataatc
     5281 cagttcacca ccacactccg gacaacagct ttcttcaggc tccagatggt gtatttcgcg
     5341 ggaaacatgt tccggtaacg gacggcgatg acgtgactgt cgcaacaggc gggggtagtg
     5401 cttccaatca gtagaacatg tgcttttcaa tgaaggagcc gatgactttt tcgtggattt
     5461 ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg gtgcggaggg
     5521 tccggttgtt acgtgcaata cgttgcgtga agattttgcc ggtcaggtgt ttctccttcg
     5581 gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag
     5641 tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
     5701 acacaccgcc agttttagtg ttgtacgcat accagagcca gtgtt
//
LOCUS       DAEBXD010000182         5737 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEBXD010000182 DAEBXD010000000
VERSION     DAEBXD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.183"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..241
                     /locus_tag="KJL94_003892"
     CDS             <1..241
                     /locus_tag="KJL94_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412054.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="KJL94_003893"
     CDS             complement(238..1179)
                     /locus_tag="KJL94_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBD4412055.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="KJL94_003894"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="KJL94_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="KJL94_003895"
     CDS             complement(1684..2904)
                     /locus_tag="KJL94_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD4412056.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="KJL94_003896"
     CDS             complement(2908..3651)
                     /locus_tag="KJL94_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD4412057.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="KJL94_003897"
     CDS             complement(3542..5008)
                     /locus_tag="KJL94_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBD4412058.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="KJL94_003898"
     CDS             complement(5008..5241)
                     /locus_tag="KJL94_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBD4412059.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="KJL94_003899"
     CDS             complement(5229..5708)
                     /locus_tag="KJL94_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412060.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1227 a         1618 c         1435 g         1457 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacatg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatga
//
LOCUS       DAEBXD010000183         5702 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEBXD010000183 DAEBXD010000000
VERSION     DAEBXD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.184"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..219)
                     /locus_tag="KJL94_003900"
     CDS             complement(<1..219)
                     /locus_tag="KJL94_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBD4412061.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="KJL94_003901"
     CDS             complement(216..1124)
                     /locus_tag="KJL94_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD4412062.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="KJL94_003902"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="KJL94_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KJL94_003903"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KJL94_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KJL94_003904"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KJL94_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBD4412063.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="KJL94_003905"
     CDS             5533..>5702
                     /locus_tag="KJL94_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412064.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAEBXD010000184         5691 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEBXD010000184 DAEBXD010000000
VERSION     DAEBXD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.185"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="KJL94_003906"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="KJL94_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBD4412065.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KJL94_003907"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KJL94_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBD4412066.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="KJL94_003908"
     CDS             complement(1477..1881)
                     /locus_tag="KJL94_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBD4412067.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="KJL94_003909"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="KJL94_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBD4412068.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="KJL94_003910"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="KJL94_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBD4412069.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KJL94_003911"
     CDS             complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KJL94_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBD4412070.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1410 c         1495 g         1290 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgcgagcac
     5461 ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt aagccgtgaa
     5521 aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt ttctcgttac
     5581 cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca tcgccttatg
     5641 ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcactc t
//
LOCUS       DAEBXD010000185         5683 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEBXD010000185 DAEBXD010000000
VERSION     DAEBXD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.186"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="KJL94_003912"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="KJL94_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBD4412071.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="KJL94_003913"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="KJL94_003913"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBD4412072.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="KJL94_003914"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="KJL94_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBD4412073.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KJL94_003915"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KJL94_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBD4412074.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1488 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtc
//
LOCUS       DAEBXD010000186         5642 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEBXD010000186 DAEBXD010000000
VERSION     DAEBXD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.187"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            263..1189
                     /gene="tauA"
                     /locus_tag="KJL94_003916"
     CDS             263..1189
                     /gene="tauA"
                     /locus_tag="KJL94_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4412075.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1202..1969
                     /gene="tauB"
                     /locus_tag="KJL94_003917"
     CDS             1202..1969
                     /gene="tauB"
                     /locus_tag="KJL94_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBD4412076.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1966..2793
                     /gene="tauC"
                     /locus_tag="KJL94_003918"
     CDS             1966..2793
                     /gene="tauC"
                     /locus_tag="KJL94_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBD4412077.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2790..3641
                     /gene="tauD"
                     /locus_tag="KJL94_003919"
                     /pseudo
     CDS             2790..3641
                     /gene="tauD"
                     /locus_tag="KJL94_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KJL94_003920"
     CDS             complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KJL94_003920"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBD4412078.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5245..5613
                     /locus_tag="KJL94_003921"
                     /pseudo
     CDS             5245..5613
                     /locus_tag="KJL94_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1326 a         1409 c         1528 g         1379 t
ORIGIN      
        1 attgacctgc ctttagggtg cctttaaaat tcgtttttaa aggctacgat gacatttttg
       61 aagagtctta aaagaattaa aatacacggc gcgcctatct tgaatgtttt atcgatatcg
      121 aagcattgag catttttaac tcgttttatc ttgaatgcaa tcggcgcata acttattgaa
      181 tcccatcaaa tcccgtcttt ttttacccat tcccggttat cttgaataaa ttacctagtc
      241 atataacaca cttcttgccg cactggcatt catcgctttt caggcacagg cggtgaacgt
      301 caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg gctcaggccg acaacacctt
      361 tgctaaagag agcggggcga gcgtggactg gcgtaagttt gacagcggag ccagcatcgt
      421 gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc ggttccagcc cattagcggt
      481 tgcagccagc caacaggtgc cgattgaagt cttcttgctg gcgtcaaaac tgggtaactc
      541 cgaagcgctg gtggtaaaga aaactatcag caaacccgaa gatttgattg gtaaacgcat
      601 tgccgtaccg tttatctcca ccacccacta cagcctgctg gcggcgctga aacactgggg
      661 gattaaacca gggctagtgg agattgtgaa cctgcagccg cccgcgatta tcgccgcatg
      721 gcagcggggt gatattgatg gtgcttatgt ctgggcaccg gcggttaacg ccctggaaaa
      781 agacggcaag gtgctgaccg attctgaaca ggtcgggcag tggggtgcgc caacgctgga
      841 cgtctgggtg gtgcgcaaag attttgccga gaaacatcct gaggtcgtga aagcgttcgc
      901 caaaagcgcc atcgatgcac agcaaccgta cattgctaac cctgacgcgt ggctgaaaca
      961 gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg cctgaaggtg acgttccggg
     1021 gctggtgaag ggaaatacct atctgacgcc gcagcaacaa acggcagaac tgaccggacc
     1081 ggtgaacaaa gcaatcatcg acaccgcgca gtttttgaaa gagcagggca aggtcccggc
     1141 tgtagcgaat gattacagcc agtacgttac ctcgcgcttc gtgcaataaa aggaggcgca
     1201 gatgctgcaa atctctcatc tctatgccga ttatggcggc aaaccggcac tggaagatat
     1261 caacctgacg ctggaaagcg gcgagctact ggtggtgctg gggccgtccg gctgcggtaa
     1321 aaccaccctg ctgaatctga ttgccggttt tgtgccttat cagcatggca gcattcaact
     1381 ggcgggtaag cgtattgagg gaccgggagc agagcgtggc gtagtttttc agaatgaagg
     1441 gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc ggcctgcaat tggcaggtat
     1501 agagaaaatg cagcgactgg aaatcgcgca ccagatgctg aaaaaagtgg ggctggaagg
     1561 cgcagaaaaa cgctatatct ggcagctttc cgatggtcaa cgtcagcggg tggggattgc
     1621 ccgcgcgctg gcggcgaatc cccagctgtt attactcgac gaaccgtttg gtgcgctgga
     1681 cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa ctctggcagg agacgggcaa
     1741 gcaggtgctg ttgattaccc acgatataga agaagcggtg tttatggcga ctgaactggt
     1801 tctgctttca tccggccctg gccgtgtgct ggagcggctg cggctcaact ttgctcgccg
     1861 ctttgttgcg ggagagtcga gccgcagcat caagtccgat ccacaattca tcgccatgcg
     1921 cgaatatgtt ttaagccgcg tatttgagca acgggaggcg ttctcatgag tgtgctcatt
     1981 aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc cgctctcgcg tcaggtgacc
     2041 ttaagcattg gcacgttagc ggttttactc actgtatggt ggacggtggc ggcgctgcaa
     2101 ctgattagcc cgctgttttt gccgccgccg caacaagtac tggcaaaact gctcaccatt
     2161 gccggaccgc agggatttat ggacgccacg ctgtggcagc atctggcagc cagcctgacg
     2221 cgcattgtgc tggcgctact cgcggcggtg gtgatcggta ttccggtcgg gatcgccatg
     2281 gggctaagcc cgacggtgcg cggcattctg gatccggtaa tcgaacttta tcgtccggtg
     2341 ccaccgctgg cttatttgcc gctgatggtg atctggtttg gtattggtga aacctcgaag
     2401 atcttactga tctatttagc gatttttgcg ccggtggcga tgtcggcgct ggcaggggtg
     2461 aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag
     2521 gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt
     2581 ggtctgggtg tgggctggtc tacgctggta gcggcggagc tgattgccgc gacacgcggt
     2641 ttaggattta tggttcagtc agcgggtgaa tttctcgcga ctgacgtggt actggcgggg
     2701 atcgcggtga ttgcgattat cgcctttctt ttagaactgg gtctgcgcgc gttacagcgc
     2761 cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg tctgagcatt accccgctgg
     2821 ggccgtatat cggcgcacaa atttcgggtg ccgacctgac gcgcccgtta agcgataatc
     2881 agtttgaaca gctttaccat gcggtgctgc gccatcaggt ggtatttctg cgcgatcaag
     2941 ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt tggcgaactg catattcacc
     3001 ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt gctggatacc cataacgata
     3061 atccgccgga taacgacaac tggcataccg atgtgacatt tattgaaacg ccacccgcag
     3121 gggcgattct ggcagctaaa gagttacctt cgaccggcgg tgatacgctc tggaccagcg
     3181 gtattgcggc ctatgaggcg ctctctgttc ccttccgcca gctgctgagc tggctgcgtg
     3241 cggagcatga tttccgtaaa tcattcccgg aatacaaata ccgcaaaacc gaggaggaac
     3301 atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt gctacatccg gtggtgcgaa
     3361 cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga aggctttact acgtgaattg
     3421 tcgatgtcag cgagaaagag agcgaagcct tgttaggctt tttattcgcc catatcacta
     3481 aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga tattgcgatt tgggataacc
     3541 gcgtgactca gcactatgcc aatgccgatt acctgccaca gcggcggatt atgcatcggg
     3601 cgacgatcct tggggataaa ccgttttatc gggcggggtg atacgagtgc ggacggttcc
     3661 ctcgccccct tggggagagg gttagggtga gggggcgttc actgtacttt caacaggtta
     3721 actccccctt tctgagagga aaaaaattaa cgcagaatct tcttctcagc caaatccagc
     3781 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa ttgaacctaa gctttcgagc
     3841 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcgtactca
     3901 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
     3961 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
     4021 agtgactcac gaatcgcctc gcgacggttc attgggttca tctgatagct tttgcggtcg
     4081 ccttttaatg cgcttcctgc agcttcacgg aacgggccat aaaaggaaga ggcgaacttg
     4141 gtcgaatacg acataatcgc cgtatcttta aagcctgcag cgtccagcgc ctgacgaatc
     4201 gcctgtacct ggccgtccat cgcggcggaa ggggcgatga agtctgcgcc tgcagcagct
     4261 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
     4321 cacagcacac cgcagtgacc gtgagaagtg tattcacaga aacaagtgtc tgacatgacg
     4381 atcatttctg gcacagtctg cttgcagatg cgcgacatac gcgccaccag tccatcttcc
     4441 cgccaggcgt cgctgccggt ttcatcggta tggtgagaga tgccaaaagt cattacggaa
     4501 cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
     4561 atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
     4621 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
     4681 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
     4741 gaattaaggt gcacagtata cctgaagcgg ggcaggagtg ttttacgaaa gttgtcgcga
     4801 tgttgacaag aagagaatga aagagagacc aggacatctt tgtaatagaa atagcaataa
     4861 tttcatattc actgaaaata tttttaatct ttatttatag ctcttggtta ttattttttg
     4921 gcgtttggtt gtgctgctat aaattgaata attcaaactt tgttgcatat tgataagata
     4981 tttatctggt ttgtgttttt gatatttata acgcgtacgc atcaaaaata acattatctg
     5041 caacatactg aaatatcgtc cttttatcaa tttgatgcgt gtttttctcc ataaattata
     5101 tatgtccaca ttcggactta ggggaaagaa taattgaacc attcgtctgt aacgcaacat
     5161 aatcgttagc gcgaaacata atatgttttc tatgcattga taattgatgg atcaacttat
     5221 tacgtccctg aggagggatg acaaatgcac tcctggaaaa agaaacttgt agtatcacaa
     5281 ttagcattgg cttgcactct ggctatcacc tctcaggcta atgcagcgac caacgatatt
     5341 tctggtcaaa cttacaatac tttccatcac tacaacgacg ccacctatgc tgatgacgtt
     5401 tactatgatg gttatgtagg ctggaacaac tatgccgctg atagctatta caacggcgat
     5461 atctacccgg tcattaataa cgctaccgtt aacggcgtga tttctaccta ctatctggac
     5521 gacggtattt ctaccaatac caacgccaat agtctgacaa tcaaaaacag cactattcac
     5581 ggtatgattt attccgagtg catgacaact gattagactg gccccctgaa tctccagaca
     5641 ac
//
LOCUS       DAEBXD010000187         5582 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEBXD010000187 DAEBXD010000000
VERSION     DAEBXD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.188"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..279)
                     /locus_tag="KJL94_003922"
     CDS             complement(1..279)
                     /locus_tag="KJL94_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412079.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="KJL94_003923"
     CDS             220..405
                     /locus_tag="KJL94_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412080.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="KJL94_003924"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="KJL94_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4412081.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="KJL94_003925"
     CDS             complement(1254..1670)
                     /locus_tag="KJL94_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412082.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="KJL94_003926"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="KJL94_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="KJL94_003927"
     CDS             1837..2877
                     /locus_tag="KJL94_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4412083.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="KJL94_003928"
     tRNA            complement(2905..2994)
                     /locus_tag="KJL94_003928"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="KJL94_003929"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="KJL94_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBD4412084.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="KJL94_003930"
     tRNA            3986..4061
                     /locus_tag="KJL94_003930"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="KJL94_003931"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="KJL94_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1505 a         1322 c         1292 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
     5581 at
//
LOCUS       DAEBXD010000188         5562 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEBXD010000188 DAEBXD010000000
VERSION     DAEBXD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.189"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(959..1335)
                     /gene="rnpB"
                     /locus_tag="KJL94_003932"
     ncRNA           complement(959..1335)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KJL94_003932"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1368..2513)
                     /gene="garK"
                     /locus_tag="KJL94_003933"
                     /pseudo
     CDS             complement(1368..2513)
                     /gene="garK"
                     /locus_tag="KJL94_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(2610..3500)
                     /gene="garR"
                     /locus_tag="KJL94_003934"
     CDS             complement(2610..3500)
                     /gene="garR"
                     /locus_tag="KJL94_003934"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBD4412085.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3530..4300)
                     /gene="garL"
                     /locus_tag="KJL94_003935"
                     /pseudo
     CDS             complement(3530..4300)
                     /gene="garL"
                     /locus_tag="KJL94_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(4316..>5562)
                     /gene="garP"
                     /locus_tag="KJL94_003936"
     CDS             complement(4316..>5562)
                     /gene="garP"
                     /locus_tag="KJL94_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBD4412086.1"
                     /translation="YADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIPGG
                     WLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPA
                     NARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGVICFV
                     LTALWIKLIHNPTDHPRMSEEELKFISENGAVVDMDHKKPGSAAASGLKLHYIKQLLS
                     NRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLG
                     GVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFG
                     ALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVGCS
                     ALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1402 a         1554 c         1297 g         1309 t
ORIGIN      
        1 tatataaaag ctcatcgaaa atcaacaatt gcttgaacag aattaaatta ccagagatga
       61 aagaattctc atcaaaaaaa atatatgatt acatcgatac aatcattgaa gattatgaaa
      121 atatcaaaga aaatgcttat ctggtcgtcc cccaaattaa ttatactatg gatttaaaca
      181 tagaagactc tagctcagaa gagttaatac cctcgagaaa gacgaaaatt ctccggacaa
      241 tggctttgag gtcggggaat ataacacata tgaagcatat aactcagaga atcaatattt
      301 taccagagag gactatacgt ataattacga ccttttaaat gcaatataga cttaataact
      361 tcttacaaag tctattatta ataccgtaga tattcatctt accctgtgca ccaaaagcaa
      421 aaacttatcg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc
      481 ttatcaggcc tacgccatct ctgcaatata ttgaatttgc gtgcttttgt aggcaggata
      541 aggcgttcac gcagcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga
      601 acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt tgggtatagt
      661 gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt
      721 tttacgtata tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agcgtcgtgg
      781 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
      841 acatcaggtg aaactgaccg ataagccgct ttcttttggg tatagcgtcg tggacagtca
      901 ttcatctttc tgcccctcca aaagcaaaaa cccgccgaag cgggttttta cgtaaaacag
      961 gtgaaactga ccgataagcc gggttctgtc gtggacagtc attcatctag gccagcaatc
     1021 gctcactggc tcaagcagcc tacccgggtt cagtacgggc cgtaccttat gaacccctat
     1081 ttggccttgc tccgggtgga gtttaccgtg ccacggactg ttaccagccg cgcggtgcgc
     1141 tcttaccgca ccctttcacc cttacctgat cccgcttgca cgggccatcg gcggtttgct
     1201 ctctgttgca ctggtcgtgg gtttcccccc caggcgttac ctggcaccct gccctatgga
     1261 gcccggactt tcctcccctc cgcccgtctc ccccgaagag gacgacgacg aagcggcgac
     1321 tgtctggtca gcttcggcgc gcagtataga gggtttgcgc gcccttgtca ccccgtgttg
     1381 cgcattccaa tcgccagtgt cgcggcaata ttacgtgaag cacggcagat attgtcataa
     1441 gccccgcgga atgcttcgtc caacgtacct atgctggtca atacgctgaa gaccgcatca
     1501 atgccatgct gatgtacaac gccaacatca tcggtcaggc tacccgcaat gccaatcacc
     1561 ggtttatggt acttcttcgc cacgtttgcg acgccaatcg gtaccttgcc gtgaatactc
     1621 tggctgtcaa tacgcccttc accggtgatc accagcgtac aatcgtgaat atgttcctcc
     1681 agattcagcg ccgtagtgac gatttcaata ccacttttca gttccgcacc aagaaacgcc
     1741 atcagcaccg cacccatacc gcccgcagct cctgcgccgg ggacatcttt tacatcaaca
     1801 tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattgtccag ctcaacaatc
     1861 atcgcttcac tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc
     1921 agcggattgg tgacatcaca agcgacgcga atgacgcaat cttttaagcg cggatcgagg
     1981 ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc aatttcattg
     2041 ccgttggcgt cgcataattt cgcccccagc gcctgtacca tgcctgcgcc gccatcattt
     2101 gtagcgctac cgccaatgcc gataataatg tttgtcgcgc cgctctccag cgcctacagg
     2161 attaactcgc ctgtgccgcg tgaagtggtc acgagtggat cgcgtttttc cgcaggtacc
     2221 agctccagcc cactggccgc cgccatttca ataaacgcgg ttttgccatc gccggagatc
     2281 ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg acgttcagaa
     2341 ccctgggtgg ctgcaatcat cgcttccacc gttccttcgc caccgtcggc aaccggaata
     2401 gaaacgtact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc
     2461 tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt catacctatg
     2521 cctgttacca catgacgccg gagggcgttt ctcttattcg gcctggattc caggcccgga
     2581 ttgcaatacg ccatccgggc acgacgtcat taacgagtaa cttcgacttt cgccagtttt
     2641 tcgtagtagc acgccagggc gctatgatcc gccgttccta aaccatctgc tcgcagtgcc
     2701 tgcatcatct ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc gtgggaagta
     2761 tccagcgcat ttgccagatc cttaatatgc agatcaatac ggaagcccgg cttgaagttg
     2821 cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag tccaccgcga
     2881 attgcctgat acaccaggtc cgggttaacg cccgctttag ttgccagcgt taacgcttct
     2941 gacatcgcgg caatattcag cgccacaatg acctgattcg ccagtttggt gacgttacct
     3001 gcaccaattg ccccggtatg caccacggaa cccgccattg ctttcatcaa atcatagtat
     3061 ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc gatggctttc
     3121 ggttcaccgc cgctcaccgg agcatccagc atatcaatgc ctttcgcttt cagcgcttcg
     3181 ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac cgtacctggc
     3241 ttcgcgcctt caataatgcc attctcaccc agcgccacct ctttcacatg aggggagttt
     3301 ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt agaggctgtt
     3361 tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc aaccaccagc
     3421 gagtaacctg ctttcagaag gtttttactc attggtttac ccataatccc caggccaata
     3481 aaaccaactt tcatagtcat atcaatcatc tctcttgttg cggtggtggt tattttttaa
     3541 aggtatcagc cagtttatga gtggcagagc ggaagacgcc gagatcgctg ccgacagcca
     3601 caaacgtcgc gccccattcc agataacgac gcgcatcggc ttcgaccggc gcgaggatac
     3661 cgctgggttt gccgtgcgcg ctggcacggt taaaaatgtg ctgaattgct ttttgtacat
     3721 ccgggtgtga tgcattgccg agatggccta atgccgcggc cagatcgctg gggccgacga
     3781 agatgccgtc tacgccttcg gtagcggcaa tggcatcgac gttatctacg ccctgctgac
     3841 tttctatctg gaccagaata gtgatgttct tgttcgactg agcgaaataa tccgccacgg
     3901 tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc ggtgggtaac
     3961 gggttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga atcaggaagt
     4021 tatagaaacc gatatccaga agacgcttaa taatttccgg ctcgttggtc ggcactcaca
     4081 ctactggcgc gctggcgctg cctttcaagg ccattaactg cggaataaac gtggagatat
     4141 cgtttggcgc atgttcgcca tccagcacca gccagtcaaa cccagccaaa ccaagaactt
     4201 cagtgctaat cgggttagag agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca
     4261 gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc tttgcttatt
     4321 tctgcaattc catacgttta atgtcgccaa ctacgaagag gtagcagacc atcgccatca
     4381 gcgctgaaca tcccacgaaa accagtgctg cattgaagga gtgcagttca cttaccaggt
     4441 agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta aagacgccgc
     4501 cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat caccggccag cccagagcac
     4561 caaatccttt gccaaaaaaa gccagcgcca tcagcatgac caccagcgtg gtgttgttgg
     4621 tgtagttaca taagatgatg gtggaagcca gcaacattcc cagcacaatc ggtagcttac
     4681 gtgccagggt cagggataaa ccgcgtttga tcagataatc cgagaagaca cctcccagca
     4741 cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac cagacccact ttcagaatcg
     4801 acatgccttt ttcctgcacc agataaatcg ggaaccaggt gaggaagaac caggtgatgg
     4861 tgttgataaa atattgtccg aaaaatacgc ccaacatcat gcggttagag agcaattgct
     4921 tgatgtaatg cagtttgaga ccgcttgctg ccgcgctgcc cggctttttg tggtccatat
     4981 cgaccaccgc gccattttca gagataaact tcagctcttc ctcagacata cgtgggtgat
     5041 ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag cacaaaacaa atcaccccca
     5101 taacggtaaa gacgtgctcc catccccagg cgaaagtcag ccagccaagc agaggcgaaa
     5161 agagcgccag cgagaaatat tgtgccgagt taaagatggc ggaggcagta ccacgttctt
     5221 tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaatgatgga gcttccgaga
     5281 atcccagcat aaagcgcata aagaacatgg agatccctgc ccaggccagc gggaacatat
     5341 caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtag gtgtaaactt
     5401 ttttcgagcc aaacttatca agcaaccagc cgccggggat ttgcatcagc aagtaggccc
     5461 agccaaaagc ggagaagatg taacccatcg aaaccgcact taactgcaac tcttttgcca
     5521 cttcggtacc agcaatagac agcgttgcac gatcggcgta gt
//
LOCUS       DAEBXD010000189         5557 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEBXD010000189 DAEBXD010000000
VERSION     DAEBXD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.190"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..641
                     /locus_tag="KJL94_003937"
     CDS             <1..641
                     /locus_tag="KJL94_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBD4412087.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KJL94_003938"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KJL94_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBD4412088.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="KJL94_003939"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="KJL94_003939"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBD4412089.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAEBXD010000190         5494 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEBXD010000190 DAEBXD010000000
VERSION     DAEBXD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.191"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            331..537
                     /locus_tag="KJL94_003940"
     CDS             331..537
                     /locus_tag="KJL94_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4412090.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            632..1234
                     /locus_tag="KJL94_003941"
     CDS             632..1234
                     /locus_tag="KJL94_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412091.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            2434..3351
                     /locus_tag="KJL94_003942"
     CDS             2434..3351
                     /locus_tag="KJL94_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412092.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            3436..4308
                     /locus_tag="KJL94_003943"
     CDS             3436..4308
                     /locus_tag="KJL94_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBD4412093.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            4526..>5494
                     /locus_tag="KJL94_003944"
     CDS             4526..>5494
                     /locus_tag="KJL94_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBD4412094.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNS"
BASE COUNT         1411 a         1390 c         1332 g         1361 t
ORIGIN      
        1 cggtgatatc agtctcctcc tcgccaggaa cgggctgact cactgtaact acctccgaaa
       61 acaactctgc aattatcaga acgcctgctt ctctccctgt cctcacgaaa actatcccct
      121 ctttatcgcg cgtgcgtgcg gaagcatctt ttcgcaacaa ccacccggta taccggtacg
      181 gctcagccac cgcaacctcc ccgtttatct cctggcggtc acattcccga ttatttttta
      241 cgtttctccc cggttgttat gccggtgaag gtggcgcgct gttttcatga ccacaccggc
      301 tggtttaaca acatccggag gaacattctc atggctaccc cagtttcact gatggatgac
      361 cagatggtcg acatggcatt tatcactcaa ctgaccggcc tgacagataa gtggttttac
      421 aggctcatca gggatggggc ctttccggct cccatcaagc tgggccgcag ctcccgctgg
      481 ctgaaaagtg aagtggaagc ctggctacag gcgcgtattg cacagccccg cccgtaattt
      541 ctgaccgtta tccgttcacc cgcagcagac gtaatccccg gcctgccggc ggcattctgc
      601 tgcctgttat atccccgtga ggaatatgaa aatgaaacaa cagtatcaga cccgctacga
      661 atggctccac gaaagctacc agaaatggct gaccggcttc acccggcacg ccgtatcctg
      721 gggcgtgtgt catccgaata tctactactt ccataatctg acgcccggtt gggggtcatt
      781 caatggtgaa aaaccggaga ttgccatcgt accccagagc ctgcaccggc tgatttatgg
      841 ccctgacaaa cgggccacgc cgcccctgga tgatgatctg atagtgaatt tatgcaccag
      901 tgaacatctg ctggttcatc atccgatgct ggaaggcatt ctcctgtctg aatgtgaacg
      961 tctgaggcag cgttctctgg caaataaact gatcagcctc ttccgtcagt ttggtggcac
     1021 ggagttgcgc ctcaaactgg tctggctttg ctggcttgat ttaatgaccg gaaacagcct
     1081 tgaagactgg aaagagaacc tgaaacggaa atcagaaaaa gagctggagg aatggatcat
     1141 caaccgtcag aggcagagcg cagcactgac ggatctgatg gatcagtacg tgctcctggc
     1201 ataccgcaca acggttgacg atagccgcaa ctgatgccgg ttgcttccgg aaggtgtgac
     1261 aaaatacgga aatgatgatg ctcccggccc ccatgctcac ctgcagagcg tgttcgtcgt
     1321 catcatactg agccatgacg ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca
     1381 ccaccagagg agccacggac agggatgttc tttacagccc gatacacgcc aggttctcag
     1441 cagactgaat gtgcaggtgc ggttattcac cagaaatggc atgctgcggt acatacccat
     1501 cctggtgaca atatgtacgt ggcgacagcg atcccggcac tgtggaagag actcactcag
     1561 cctgcaggat attctttccg gcatccggga atgtccgcct gaatgcaaac aggatgatat
     1621 ttgaatccgt aatactgttc tttcatcata aataatttat gcagatacag ttaaccgtta
     1681 cagtagccgg tttgttttac cgggaaataa tatccgtcag actgataccg gaacatatct
     1741 gaaacacgtt atgatgtttt gttatcagaa atgttgtata ttcttatccg tggcgacagt
     1801 ttaatgccct ctggtatcac ctgataatgt atatcctgcg atgattcgtt atatttacgg
     1861 gacacgcctc ttttcattgt gctccttttc agacttcatt ttccgtaagt gttccccggc
     1921 ataaaggagg tacctttctg gcgtttataa ctctctttat cggtgacgcc ggtaatacag
     1981 ggaataccac accgacaaac gaggtattaa cagacgatat ttcagtgtcc ataatttttc
     2041 tgtccggtta aataacgtca gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc
     2101 ataccgggtt acaacgtaaa aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac
     2161 atgtttacct ttcaataccc cctgtctgca gtctccactc ttccatcctc ttaagctacg
     2221 tcggccacat gagtgcactt ccatgataac aggtattcct gtcagcataa cgaagccggt
     2281 taccttccct tcctcctgca tttcctgtca gaatgactat acagcgcgca gattgccttt
     2341 ttattgacca tcttaatata ccgttataaa cttcttttta tcatattatt atcaccttat
     2401 tatatttaat ttttaagcag tcggagaaag aggatggttc agaaaatttt gtcggataag
     2461 gttatgaatg aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc
     2521 gtactgcccc ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac
     2581 agagaaaatc tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg
     2641 cgtctgaagg ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt
     2701 gaacgcgcat gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca
     2761 aaaataagcg cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa
     2821 gggaatgcct tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat
     2881 attctttctg ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg
     2941 cgggcattta aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc
     3001 atatggacat ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt
     3061 cctggttctg ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt
     3121 ccactcacct cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg
     3181 cagcgcaagt acaaaaaaat gcaggaagat aaagtcaatg tacagttttt tcttgacgca
     3241 gaaaccaggg cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg
     3301 ctgcatgacc tgattgttga agagtataaa aggtaccgac attccagata atttcagaca
     3361 tatattatct ccgtgagtcc cccgccacct ttccggtgtg cgggggtttt gtcttttttc
     3421 accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc
     3481 tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact caccagtcac
     3541 gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgca
     3601 ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg ggaagtgcag
     3661 cgtttccgtc tgagtggcca tggtcacagc atggttatca ctgacctgcc cggggtgggc
     3721 gagagccggg acagagatgc agagtatgaa gccctgtacc gtgacattct gcctgaactt
     3781 gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc
     3841 tggcgacaca tcctgcaccg gggacatcag caggtgctgt ttgtggtgac gcaggccgac
     3901 aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc cgcacaggca
     3961 cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt acatccggtt
     4021 gttgccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc gctaatgaca
     4081 gcacttcccg accatgccgc cagtcccctg atgacccgcc tgcaggacgg gctgcgcacg
     4141 gaatctgtcc ggggtcaggc ccgtgaacag tttaccggtg cggtggaccg gatatttgac
     4201 acggcggaga gcgtctgtgt tgcctctgtt gcccgtaagg ttctgcgcgc tgttcgtgac
     4261 accgtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac ccggtggtga
     4321 tgcaccagac tcttgttaca gatttttaac cccatgaaaa cacaatgcaa acacagccat
     4381 acccgggttg tccccttgcg ttgtgtttct caagttaata gaatgcatcg atcgttacta
     4441 tcatatcgat cgctataaac gatcgtttgt ggtaaatgac ctcctccgtg gttgtcatga
     4501 aaatgcgtct caggaaggtt aaataatgca cgaaaagaac atcgccctgc tttgtgatga
     4561 agccgaccga cttttgcaac tgaacattaa tctgctccgg caaatggttg atgaaccgga
     4621 tgtgttatct gacagtaaga acgaaaacag actgcttttt gataaacaga aagcactgaa
     4681 aagaattgag gagctggagg gcgaacaaat caaaaccgcc cgcagggaga tggtgctggc
     4741 tgttgtcggc acgatgaaag caggtaaatc aactaccatc aacgccattg tggggcagga
     4801 aattctgcct aaccgtaacc gccccatgac ctctgtaccg acgctcatcc gccacgttcc
     4861 cggaaaaact gagccggtcc tccatctgga acatattcag cccgtccgca atttattaag
     4921 cacactgcaa caaaaactcg acaccccggc aggacagcaa gtcgcacaga ccctgcagca
     4981 aaccggggat acctgcgaac tgctggatat tctggcaaat gatggctggc tcaaaaatga
     5041 ataccacggg gaagacgaaa tctttaccgg actggcatcg ttaaacgatc tggttcgcct
     5101 tgctgcggca atggggagtg agtttccttt tgatgaatac gcagaagtgc agaaactgcc
     5161 ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg gaacactcac
     5221 actgctagat acccccggcc ctaatgaggc cggacaaccg cagatggaag tgatgatgcg
     5281 ggatcaactg cagaaagcct ctgcggtgct ggcggtgatg gattacaccc agatgaactc
     5341 aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat cagccggccg
     5401 cctgtttgtg ctggtcaata aatttgatga gaaagaccgc aatggcgatg gggcagatgc
     5461 cgtacgccag aaagttccgg caatgctgaa cagc
//
LOCUS       DAEBXD010000191         5447 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEBXD010000191 DAEBXD010000000
VERSION     DAEBXD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.192"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..264)
                     /locus_tag="KJL94_003945"
     CDS             complement(<1..264)
                     /locus_tag="KJL94_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBD4412095.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVI"
     gene            485..913
                     /gene="uspG"
                     /locus_tag="KJL94_003946"
     CDS             485..913
                     /gene="uspG"
                     /locus_tag="KJL94_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBD4412096.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1034..2599)
                     /gene="ahpF"
                     /locus_tag="KJL94_003947"
     CDS             complement(1034..2599)
                     /gene="ahpF"
                     /locus_tag="KJL94_003947"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBD4412097.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2844..3407)
                     /gene="ahpC"
                     /locus_tag="KJL94_003948"
     CDS             complement(2844..3407)
                     /gene="ahpC"
                     /locus_tag="KJL94_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBD4412098.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3779..4525
                     /gene="dsbG"
                     /locus_tag="KJL94_003949"
     CDS             3779..4525
                     /gene="dsbG"
                     /locus_tag="KJL94_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBD4412099.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4735..>5447
                     /locus_tag="KJL94_003950"
     CDS             4735..>5447
                     /locus_tag="KJL94_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4412100.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINS"
BASE COUNT         1448 a         1323 c         1264 g         1412 t
ORIGIN      
        1 aatgacgaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt ccttcccggt
       61 ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat
      121 tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttaccgtaa tacgcagaat
      181 aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg
      241 gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct
      301 ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattccta
      361 gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa
      421 tatcagggat aaatattata atcatggaga atgatttgtt catgattaac agggagaaag
      481 ggttatgtat aaggcaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa
      541 agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt
      601 actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga
      661 agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat
      721 cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga
      781 gttggcagaa gaactggggg ctgatgttgt agttatcggt tctcgcaacc catcgatttc
      841 gacccatctg ttaggttcta acgcctcgag cgtgatccgc cacgccaatc tgcctgtact
      901 ggtcgttcgc taacagataa aagtatttat gccgccctgc ctggaacatc ccggcagggc
      961 tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga
     1021 atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg
     1081 ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac
     1141 ccgctgcgaa cacgcctttg acgttggttt cacatttcgc atcaatgata atctcgccca
     1201 tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga
     1261 caaaaatacc ggccagttcg atgttgtgaa tattgccgct gacacgatct cgatattcca
     1321 gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt
     1381 cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg
     1441 caaattccag cagcgttacg tgctcaacga tacccgccag gtcgattgcc gcttccacgc
     1501 cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggt ccgtcgcagt
     1561 gcgggcagta ggtcacgcct ttagtgcgat actgatcttc gcccggaacg ttcatgttgc
     1621 gccattttgc acccgtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg
     1681 tttcaatctg gtgtagacca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc
     1741 tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccaacttct
     1801 gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac
     1861 caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg
     1921 ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca cgcttgttca
     1981 gctcttctgc cgcacgtttt tccgcgccag tatcaatttt ggcaacgatt tcagtcaacg
     2041 tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt
     2101 tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg
     2161 ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta tggcaagaga
     2221 gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag
     2281 actgcgcttc tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga
     2341 actcgtggcc cagcggggag cctgcaaaac gtggtccctg gttggaacct gggttggtga
     2401 tcaggaaaga cggcttacgc actggcaagc tgttatcttc tttaaaggtg actttgtctg
     2461 acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca
     2521 gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt
     2581 tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct
     2641 ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc
     2701 acccgaagaa ttaggtgaat ttccgaagta tttcgcgtca gagcaaggcg actagcctgt
     2761 gaatccccgg gagcttacac aagtaagtga ccggggtgag cgggcgacgc caacgccgct
     2821 atggcgtgaa agacgaagga aatttagatt ttaccaacca ggtccagaga cggagccaga
     2881 gttgcttcac cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac
     2941 tgagctgctt tgattttacg cagcagatca gacgcgtcac ggccaatgcc ttcagcggta
     3001 acttcgattg cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga
     3061 ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc
     3121 atcgcatatt tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag
     3181 tgagtatcgg tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg
     3241 tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag
     3301 aagacgctcc agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg
     3361 aatgcctggt ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc
     3421 gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt
     3481 atcaaatcga taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg
     3541 ccagcggcga taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc
     3601 ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg
     3661 acatggatta caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat
     3721 gacagtgata ggttatgcct tttactcgac ttttgcactg actgaaaaga acaaattaat
     3781 gttaaaaaag atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc
     3841 ttccccagta aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc
     3901 cggaggaatg aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac
     3961 tcccgatggt aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag
     4021 taacacactt atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat
     4081 ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtctt
     4141 ctccgatccg ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga
     4201 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
     4261 gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatatgaagc
     4321 ctctggttgc aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt
     4381 gttaagtgac aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta
     4441 ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct
     4501 taatatcatt atgggaaata aataaaaggt aattcatgga tggttgaact atatccctgc
     4561 ttttcaacca tcctaaaaaa gcatgaattt ctcgctctac cctaagtggc tgtgctattt
     4621 tagaaaaact taatcctgag ttaagcaaaa aaacataatc aataaaaata catacgcaaa
     4681 aaacattgac taagtgaata taccatggaa gaaaaatata aaccggagta gtgtatggcc
     4741 aatctctacg acttgaaaaa gttcgactta aatcttcttg tcatatttga gtgtatttac
     4801 cagcatttaa gcattagcaa agctgctgaa tcactatata tcaccccatc agccgtgagc
     4861 cagtcgttac aacgcttacg tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa
     4921 ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc atcttgagaa aaacctcaaa
     4981 ggccttgaac aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc
     5041 tacggccctc agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa
     5101 gatgcctctg tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa
     5161 ctgttggttc accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc
     5221 gtcatttgca tgccattgca tacaattcgc aatactctaa tttgtagtaa taagcatccg
     5281 cgaattactg acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata
     5341 gccaaatcag caggagttga tgatattcaa atggaaatag acgagaagtt tatgaatcgc
     5401 aaaatttcat ttcgtggttc ctctttgctg acaataatta atagcat
//
LOCUS       DAEBXD010000192         5417 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEBXD010000192 DAEBXD010000000
VERSION     DAEBXD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.193"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..511
                     /locus_tag="KJL94_003951"
     CDS             <1..511
                     /locus_tag="KJL94_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBD4412101.1"
                     /translation="RGAYNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQ
                     TRLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDM
                     AAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGM
                     DMSHAESAH"
     gene            533..2524
                     /gene="cyoB"
                     /locus_tag="KJL94_003952"
                     /pseudo
     CDS             533..2524
                     /gene="cyoB"
                     /locus_tag="KJL94_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2514..3128
                     /locus_tag="KJL94_003953"
     CDS             2514..3128
                     /locus_tag="KJL94_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBD4412102.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3128..3457
                     /locus_tag="KJL94_003954"
     CDS             3128..3457
                     /locus_tag="KJL94_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBD4412103.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3469..4359
                     /gene="cyoE"
                     /locus_tag="KJL94_003955"
     CDS             3469..4359
                     /gene="cyoE"
                     /locus_tag="KJL94_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBD4412104.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4544..4855
                     /locus_tag="KJL94_003956"
     CDS             4544..4855
                     /locus_tag="KJL94_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD4412105.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4839..4997
                     /locus_tag="KJL94_003957"
     CDS             4839..4997
                     /locus_tag="KJL94_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD4412106.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4990..5388
                     /locus_tag="KJL94_003958"
     CDS             4990..5388
                     /locus_tag="KJL94_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4412107.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1183 a         1346 c         1430 g         1458 t
ORIGIN      
        1 tcggggcgct tacaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac
       61 ctccaactcc gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat
      121 ggccggtatg cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat
      181 ctccgccagc tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc
      241 ggatcgcgcc gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga ataccatgtc
      301 tgacatggct gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt
      361 ctccaacgtg aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa
      421 gagcatggac atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat
      481 ggacatgagc cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg
      541 aaaattatca cttgatgcag tcccgttcca tgaaactatc gtcatggtta cgatcgctgg
      601 cattattttg ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta
      661 cctgtggaaa gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat
      721 cgtggcgatt gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca
      781 ggctcttgcc tcggcgggtg aagcgggctt cctgccacct caccactacg atcagatctt
      841 caccgcgcac ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat
      901 gaacctggtg gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa
      961 cttaagcttc tggtttactg ttgttggcgt gattctggtt aacgtttctc tcggcgtggg
     1021 cgaatttgcg cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc
     1081 gggagtcggt gttgattact ggatatggag tctccagcta tccggtatag gtacgacgct
     1141 taccggtatc aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt
     1201 caagatacca gtatttacct aggcatcact gtgcgcgaac gtactgatta ttgcttcctt
     1261 cccaattctg acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt
     1321 ctttaccaac gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg
     1381 ccacccggaa gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc
     1441 aaccttctcg cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat
     1501 caccgtgctg tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa
     1561 cgtaaacgcc ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat
     1621 cttcaactgg ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg
     1681 gactatcggt tttatcgtca ccttctcggt gggcggtatg actggcgtgc tgctggcagt
     1741 accgggcgcg gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt
     1801 gatcatcggc ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc
     1861 gttcggtttc aaactgaacg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg
     1921 cttcttcgtt gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt
     1981 gagccagcag attgacccgc agttccacac catgctgatg attgcagcca gcggtgcagt
     2041 actgattgcg ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg
     2101 cgaccagaac cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac
     2161 ctcttccccg cctccgttct ataactttgc cgtagtgccg cacgttcacg aacgtgatgc
     2221 attctgggaa atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat
     2281 tcatatgccg aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg
     2341 tttcgccatg atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat
     2401 cacctggatc gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat
     2461 cgaaaaactg gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa
     2521 ctgatacttt gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg
     2581 gaaccaaaat cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct
     2641 tgtttgctac ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca
     2701 ttttcgaact gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct
     2761 acggcatggc ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg
     2821 cgttgacctg gttgtttggt gccggattta tcgggatgga aatctatgaa ttccatcacc
     2881 tgattgttaa cggcatgggg ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg
     2941 tcggcacgca cggtctgcac gtcacttctg gtcttatctg gatggcggtg ctgatggtgc
     3001 aaatcgcccg tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt
     3061 tctggcactt cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg
     3121 cgatgtaatg agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta
     3181 catgacaggc tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac
     3241 aggggctgcc tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt
     3301 tctggtgcat ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat
     3361 gacggcgttt gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat
     3421 tatgtggaac ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag
     3481 caatacctgc aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg
     3541 ggattcctgc tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt
     3601 ggggtgtcac tggttgtgac gtcgggttgt gtgtttaaca actacatcga cagggatatc
     3661 gacagaaaga tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct
     3721 gtctcgctgg tgtacgccac cttgctgggt attgctggct ttatgctgct gtggtttggc
     3781 gcgaatccgc tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtctat
     3841 agcctgtaca tgaaacgcca ctcagtctac ggcacattga ttggttcgct ctccggcgct
     3901 gcgccgtcgg tgattggcta ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc
     3961 ctgctggcta tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc
     4021 tttaaggatt accaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg
     4081 gcgaagaatc acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct
     4141 cttggcggtt acgctgggta taaatatctg gtggtcgccg cggcggttag cgtctggtgg
     4201 ttaggtatgg ctctgcgcgg ttataaagtt gctgatgaca gaatctgggc gcgcaagctg
     4261 ttcggcttct ctatcatcgc catcactgcc ctctcggtga tgatgtccgt tgattttatg
     4321 gtaccggact cgcatacgct gctggctgct gtgtggtaac aaaacctctc tattaaaaag
     4381 gtgctacggc acctttttta ttgtctgaga tcagagatac ggctaataac cgtatctctt
     4441 gacagagtga gcagatgcac ataaattaat caaacggttg atagccgtat tatgtatgag
     4501 atcgactccc tacgatttca ttcagttaat caaagaagtc ataatgcctg gaaaaacagc
     4561 cgctctatac gatgtcgata aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc
     4621 cgacgccaaa aacaaacttc gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt
     4681 tattttgagc gctgctatgg aacgcgccga aagtgtgctt gataaccaac gtcgccgtga
     4741 gctttcgaat caaagctggg aactgatgaa ccaactcatc gctgaacctg ctcaaccgac
     4801 gctcgccctc aaggcgttaa tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga
     4861 ttactctgcc catagtcctc tcctgtaatt atcagtctga tattacttat cctgggcaaa
     4921 aacagtttga ttgcggtaac cctgttatcg ataaatttgt acgcgctacg cgcatcgcta
     4981 aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt
     5041 gaactgattg gcatctgtac ttttacggca tattcgctgg aaagacaacg cgtttctggc
     5101 gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggga
     5161 gcgcggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat
     5221 gtaaaaaaaa ttcacccggc attaccaatt aaaggggttt atcttgatgc tgaccctgcc
     5281 gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt
     5341 ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc
     5401 acccgccagg gcaagtt
//
LOCUS       DAEBXD010000193         5379 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEBXD010000193 DAEBXD010000000
VERSION     DAEBXD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.194"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            61..4155
                     /gene="sepA"
                     /locus_tag="KJL94_003959"
     CDS             61..4155
                     /gene="sepA"
                     /locus_tag="KJL94_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HBD4412108.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            complement(4324..4938)
                     /gene="istB"
                     /locus_tag="KJL94_003960"
                     /pseudo
     CDS             complement(4324..4938)
                     /gene="istB"
                     /locus_tag="KJL94_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(4970..>5379)
                     /locus_tag="KJL94_003961"
     CDS             complement(4970..>5379)
                     /locus_tag="KJL94_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412109.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
BASE COUNT         1408 a         1288 c         1371 g         1312 t
ORIGIN      
        1 gactgctcca tctacatcct atgtaattaa tctttgtcaa aattaggttg atgtttctat
       61 atgaacaaaa tatattatct taagtattgc catataacca aaagcctgat tgctgtctcc
      121 gaactggccc gcagggttac ctgcaaaagc catcgcagac tttcacgtcg ggttattctt
      181 acgtctgttg cagctttatc actttcctcg gcatggccag ctctgtcagc aacggtcagt
      241 gcagagatcc cttatcagat attccgcgac tttgccgaga ataaaggtca gtttacgccg
      301 gggaccacga atatttccat ttacgacaag cagggcaatc tggtcggtaa acttgataaa
      361 gccccgatgg ctgatttcag tagtgccact attactaccg gtagcctgcc tccgggagac
      421 catacacttt actcccctca gtatgtggtt accgccaaac atgtcagcgg ttctgacacg
      481 atgagcttcg gatacgccaa aaacacctat actgcggttg gtacaaataa taatagcggt
      541 cttgatatta aaacccgccg cctgagtaaa ctggtgacag aggtggcgcc ggcagaagtg
      601 tctgacatcg gggctgtgag cggtgcctat caggccggcg gacgctttac tgaattctat
      661 cgccttgggg gggggatgca gtacgtcaaa gacaaaaatg gtaatcgcac ccaagtgtat
      721 accaatggcg gatttcttgt tgggggaacc gtcagcgcgc tgaactccta taacaacgga
      781 cagatgatta cagctcagac cggtgacatt tttaatcccg cgaatggacc tctggctaat
      841 tatctgaata tgggggacag cggctccccc ctgtttgctt atgattccct gcaaaaaaaa
      901 tgggtactga ttggcgtgct ttcctcagga actaactatg gaaataactg ggtcgtcacc
      961 actcaggatt tccttggtca gcaaccgcaa aatgattttg acaaaactat agcctacacc
     1021 tctggtgaag gagtactgca gtggaaatat gatgcggcta atggcaccgg cacactgact
     1081 cagggaaaca cgacttggga tatgcatgga aagaaaggaa atgatctgaa cgcaggaaaa
     1141 aacctcctgt tcaccggcaa taatggcgag gtcgttctgc agaattccgt taatcaggga
     1201 gctgggtatc tgcagtttgc cggcgattac agggtgtccg ccctgaacgg ccagacatgg
     1261 atggggggcg ggattatcac cgataaagga acccacgttc tgtggcaggt gaacggagtt
     1321 gccggtgata acctgcataa aactggcgaa ggtaccctga cagtaaacgg gaccggtgtg
     1381 aatgcaggcg gactcaaggt gggggacggt accgttattc tcaaccagca ggcggatgct
     1441 gacggaaagg tacaggcttt cagctctgtt ggtattgcca gcggtcgtcc gacagttgtg
     1501 ctttctgact cacagcaagt taatccggat aatatttcgt ggggataccg tggaggacgt
     1561 ctggaactga atggtaataa tctgaccttt acccgtcttc aggcggctga ctacggggcc
     1621 atcattacca ataacagtga gaaaaaatca acggtaacac tcgaccttca gacattaaaa
     1681 gccagtgaca tcaatgtacc ggttaatact gtcagtattt ttggggggag aggagcgccg
     1741 ggagacctat attatgacag ttctaccaag caatatttca tcctgaaagc gagttcatat
     1801 tccccgttct tctccgatct gaacaacagc agtgtctggc agaatgtcgg aaaagatcgt
     1861 aataaagcca ttgatactgt gaagcaacaa aaaattgagg ccagcagcca gccttatatg
     1921 tatcacggcc aactaaacgg caatatggat gtgaatattc cgcagctctc aggtaaggat
     1981 gtactggctc ttgatggttc agttaacctg ccagaaggtt cgataaccaa gaagtccggc
     2041 acgctgatat tccagggtca tccagttatt catgctggaa cgacgacttc ttccagccag
     2101 agcgactggg aaacccgtca gtttacgctg gaaaaactga aactcgatgc ggcaacattc
     2161 catctgtcca gaaacggcaa gatgcaggga gatattaacg ccaccaatgg aagtacagtc
     2221 attctgggaa gtagccgtgt ctttactgac aggagtgacg gaaccggtaa tgcggtctcc
     2281 tctgttgaag ggagtgccac tgcaaccaca gttggtgacc agagtgatta cagcggaaat
     2341 gttacgctgg aaaataaatc atccctgcaa atcatggaga gattcaccgg tggtattgag
     2401 gcttatgata gcaccgtgag tgtgacctct cagaatgctg tttttgaccg tgttggcagc
     2461 tttgtcaaca gcagcctgac cctcggaaaa ggagcaaaac ttacggctca gagcggtatt
     2521 ttcagcaccg gggctgtgga tgtaaaagaa aatgcttctc tgaccctgac ggggatgcct
     2581 tctgcgcaga aacaggggta ttactcaccc gtaatttcta caacagaagg gattaacctc
     2641 gaagataacg ccagcttttc tgttaaaaat atgggctatc tgagttcgga tattcatgca
     2701 ggaaccactg cggcaaccat taatctggga gacagtgatg ctgatgcagg gaagacggac
     2761 tctccgttat tcagctcctt aatgaaggga tataacgctg ttctgagagg cagtattacg
     2821 ggggcacaga gtacggtaaa tatgatcaat gctctgtggt actctgacgg aaaatcagag
     2881 gccggtgcac tgaaggctaa gggctcgcga attgaactgg gggacgggaa acactttgcc
     2941 actttacaag taaaagagct ttctgcagat aataccacgt tcctgatgca taccaacaac
     3001 agccgggctg accagttgaa tgtcacagac aaactgtcag gcagtaataa tagcgtcctg
     3061 gttgactttt taaacaaacc agccagtgaa atgagcgtga cgttaattac cgcaccgaaa
     3121 gggagtgatg agaaaacgtt cactgccggt acgcagcaga ttggtttcag taacgttacg
     3181 ccagtaatca gcacggaaaa aacggatgat gccacaaaat gggtgctgac aggatatcag
     3241 actaccgctg atgccggagc ctcgaaagcc gcaaaagact ttatggcatc aggttataag
     3301 tccttcctta cagaggtcaa taacctgaac aaacgtatgg gtgacctgcg ggatactcag
     3361 ggggatgccg gtgtctgggc acgcataatg aatggtaccg gttcggcaga tggtgactac
     3421 agcgataact acactcacgt tcagattggt gtcgacagaa agcatgagct ggacggtgtg
     3481 gatttattta cgggggcatt gctgacctat acggacagca atgcaagcag ccacgcattc
     3541 agtggaaaaa acaaatccgt gggtggcggt ctgtatgcct ctgcactctt taattccgga
     3601 gcttattttg acctgattgg taaatatctc catcatgata atcagcacac ggcgaatttt
     3661 gcctcactgg gaacaaaaga ctacagctct cattcctggt atgccggtgc tgaagttggt
     3721 tatcgttacc acctgacgaa agagtcctgg gtggagccac agatagagct ggtttacggt
     3781 tctgtatcag gaaaagcttt tagctgggaa gaccggggaa tggctctgag catgaaagac
     3841 aaggattata acccactgat tggccgtact ggtgttgacg tgggaagagc cttctccgga
     3901 gacgactgga aaatcacagc tcgagccggg ctgggttatc agttcgacct gctggcgaac
     3961 ggagaaacgg ttctgcagga tgcttccgga gagaaacgtt tcgaaggtga aaaagatagc
     4021 aggatgctga tgacggtagg gatgaatgcg gaaattaagg ataatatgcg tttgggactg
     4081 gagctggaga aatcagcgtt cgggaaatat aatgtggata atgcgataaa cgccaacttc
     4141 cgttatgttt tctgacgaat gggataggtc tgccattcgg caggcctttt tctgaaagaa
     4201 ctccgtgagc ctgtttaaga ttctgtgtaa atgccttttc tcagaagtga ccgtccaggt
     4261 attagacgtc aactagtttt gccgactggc gccaactata ctggccgccc cttcagtgcg
     4321 acatcaagtc acgcttgtta ctgccttcgc tgtctttttc ctgtagcttt cccctttcag
     4381 ttcgaacata tatccgtgat ggatcagtcg gtctgctgcc gccaccgcca tagtctcatc
     4441 cacgaagatg ctgccccaca tgctgaacgg atggttactg gttatcacta ggctcccacg
     4501 ttcatagcga tgcgctatta actcgaacag cacacccgtt tcggcgctgt cgcgtttgac
     4561 atagccaaga tcatccacca ctatcacccg gtagcgatcc agtttcagta gcagctcatt
     4621 cagtttcaac tgagctctgg ctttacgtag ttcctgcaac agctctcctg cgctgtagaa
     4681 ccgggcccgg tagccctggc ctactacgcc atccacgatc gctgccgcca gatggctttt
     4741 ccccaacccg ctggccccga acagcagaac gttttctcca gcatcaaccc agtctgtcgt
     4801 ttcacagagt tgctggaact gagctccatt cagttccggc aactgaatga agtcgtattc
     4861 gcttagcgtt ttggcaactg gcaaccgggc ctccttttta taacgacgca gtttttcact
     4921 ctcgcgccac aggagttctg taagcgtcaa cggaaggccg tattgacgct tatttatcgg
     4981 tgagaactac gttccatggc agcagttcgt caactcggtt ggaaggccat tccggcagta
     5041 cgctcaggat atggcgcaga tacgcttctg gatcgatacc gttcaaccga cagctcccga
     5101 ttagtccgta cagcagagct ccgcgatcgc ctccatgatc gctgccgaag aacatgtaat
     5161 tctttttccc gagacagacg gtacgaagcg ctctttctgc tgtgttattg tccgcctccg
     5221 ccagaccgtc atcactgtaa taacagaggg catcccactg attcagtaca tagctgaacg
     5281 cttcgcccag tctggatttt ttcgacagcg taccattctt ctccaccatc cattcattca
     5341 gcgacgtcag taacgctttg cttcgctgct gcctgacgg
//
LOCUS       DAEBXD010000194         5364 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEBXD010000194 DAEBXD010000000
VERSION     DAEBXD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.195"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..361)
                     /locus_tag="KJL94_003962"
                     /pseudo
     CDS             complement(1..361)
                     /locus_tag="KJL94_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="KJL94_003963"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="KJL94_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="KJL94_003964"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="KJL94_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="KJL94_003965"
     CDS             complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="KJL94_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBD4412110.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="KJL94_003966"
     CDS             complement(2629..2943)
                     /locus_tag="KJL94_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412111.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="KJL94_003967"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="KJL94_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="KJL94_003968"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="KJL94_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBD4412112.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4503..4640)
                     /locus_tag="KJL94_003969"
                     /pseudo
     CDS             complement(4503..4640)
                     /locus_tag="KJL94_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4722..5020)
                     /locus_tag="KJL94_003970"
                     /pseudo
     CDS             complement(4722..5020)
                     /locus_tag="KJL94_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1458 a         1158 c         1077 g         1671 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt
      181 tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag
      241 tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct
      301 gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca
      361 tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgcccgcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gagcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcca
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg atgaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc
     3001 cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag
     3241 tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attatttttc attcctttat tttcattagc agtattgctg cctattatta
     4081 ggtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa caataacctt tttttgtaat gtaatagata ttagtctatt
     4201 gctaatctaa aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga
     4261 tttcgtatat tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt
     4321 ttaccattct gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg
     4381 ataataaagc agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat
     4441 gctgcctaga tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac
     4501 cgctacactt tgtccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag
     4561 tgacgaactt ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc
     4621 tcatgaagag ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc
     4681 ctgaaccagt taatccatcg cccgacaaat ttagtaatct agggctggtt tttcttcagc
     4741 tgttgcggtt cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct
     4801 ccagtagtga taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga
     4861 tttttctcgc acccaagatg atgagtcata ttagcattga gtacggtttc attttcagca
     4921 tccaatcaaa ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta
     4981 aagcccgcaa cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata
     5041 tcaaattaaa tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc
     5101 ttcgaatcct gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt
     5161 atatgataaa taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga
     5221 ataaatattc tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg
     5281 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     5341 ataaaggggc ttggcagttt ggat
//
LOCUS       DAEBXD010000195         5337 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEBXD010000195 DAEBXD010000000
VERSION     DAEBXD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.196"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="KJL94_003971"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="KJL94_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBD4412113.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="KJL94_003972"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="KJL94_003972"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBD4412114.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="KJL94_003973"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="KJL94_003973"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBD4412115.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="KJL94_003974"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="KJL94_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBD4412116.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1456 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAEBXD010000196         5242 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEBXD010000196 DAEBXD010000000
VERSION     DAEBXD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.197"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="KJL94_003975"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="KJL94_003975"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBD4412117.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="KJL94_003976"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="KJL94_003976"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBD4412118.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3324)
                     /locus_tag="KJL94_003977"
                     /pseudo
     CDS             complement(2698..3324)
                     /locus_tag="KJL94_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3454..5214
                     /locus_tag="KJL94_003978"
     CDS             3454..5214
                     /locus_tag="KJL94_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBD4412119.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1338 a         1354 c         1253 g         1297 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcat attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaacca gtacaaaggt
     3241 ataaagacta cgtagtgcaa cttcgccaag gaaatcaaaa ggcactttat caaatgccat
     3301 acggtggaga tcgaacgcct tcatttttat gctcttaacg tggtttatcc gctaaaagca
     3361 tagtcaacag ttcatcggca tggcggcggt gatagcgcct ttgagataaa acccgtatta
     3421 ttgcgcgctt tccgtacgac taaagtgatt ttcatgacgc aaacatttat ccccggcaaa
     3481 gatgccgctc tggaagattc catcgctcgc ttccagcaaa aactttcaga cctcggcttt
     3541 cagattgaag aggcctcctg gctgaatccc gtgcctaacg tctggtctgt acatattcgc
     3601 gacaaagagt gcgcactgtg ttttaccaac ggtaaaggcg caaccaagaa agcggcgctg
     3661 gcttctgcac tcggtgaata tttcgagcgt ctctcaacca actacttttt tgctgatttc
     3721 tggctgggcg aaaccatcgc caacggtcca tttgtgcatt atcccaacga aaaatggttc
     3781 ccactgagcg aaaatgacga tgtgccagaa gggctgctcg atgaccgtct gcgcgcattt
     3841 tacgatccgg agaatgaatt gaccggcagt atgctgattg acctacaatc cggtaacgaa
     3901 gatcgtggta tttgcggtct gccgtttacg cgccagtccg acaatcagac cgtttatatt
     3961 ccgatgaata tcattggtaa cctgtacgtt tctaacggta tgtccgctgg caatacccgc
     4021 aacgaagcac gcgttcaggg gttgtccgaa gttttcgaac gctacgtgaa aaaccgcatt
     4081 attgctgaaa gcatcagctt gccggaaatc ccggcagacg tgctggcgcg ttacccagcg
     4141 gtggttgaag cgatcgaaac actggaaaca gaaggtttcc cgatcttcgc atatgatggt
     4201 tcccttggcg gccagtatcc ggtgatttgc gtggtactgt ttaatcctgc aaacggcacc
     4261 tgctttgcct ctttcggtgc gcatcctgat tttggcgtag cactggaacg taccgtgacc
     4321 gagctgctgc aaggtcgtgg cctgaaagat ttggatgtgt ttactccgcc aaccttcgat
     4381 gatgaagaag tcgctgaaca taccaacctc gaaacgcact ttatcgattc cagcggttta
     4441 atctcctggg acctgttcaa gcaggatgcc gattatccgt ttgtggactg gaatttctcc
     4501 ggcaccacgg aagaagagtt tgctacgctg atggctatct tcaacaaaga agataaagaa
     4561 gtttatattg ccgattacga gcatctgggc gtttatgctt gccgtattat cgtgcctggc
     4621 atgtccgata tttatccggc tgaagatctg tggctcgcga ataacagtat gggcagccat
     4681 ttacgtgaaa cgattctttc gctaccaggc agcgagtggg aaaaagaaga ttacctgaac
     4741 ctcatcgagc aactggatga agaagatttt gatgacttta cccgcgtgcg tgagctgttg
     4801 ggtctggcga ccgggtcgga taacggttgg tacaccctgc gtatcggtga attaaaagcc
     4861 atgctggcgc tggctggtgg cgatctggaa caggctctgg tctggaccga atggacgatg
     4921 gagtttaact catcagtatt tagtccggaa cgcgccaact attatcgctg cctgcaaacg
     4981 ttgttattac tggcacagga agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt
     5041 cgcatgtacg gcgcagatgc cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg
     5101 ttttacggcc tgcaaccggt agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg
     5161 ttgaaggcct acgaaaagct gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa
     5221 atagcctacg caatgtaggc tt
//
LOCUS       DAEBXD010000197         5149 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEBXD010000197 DAEBXD010000000
VERSION     DAEBXD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.198"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            188..445
                     /locus_tag="KJL94_003979"
                     /pseudo
     CDS             188..445
                     /locus_tag="KJL94_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KJL94_003980"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KJL94_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBD4412120.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KJL94_003981"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KJL94_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBD4412121.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KJL94_003982"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KJL94_003982"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBD4412122.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="KJL94_003983"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="KJL94_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="KJL94_003984"
     CDS             complement(3603..4418)
                     /locus_tag="KJL94_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4412123.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5149
                     /locus_tag="KJL94_003985"
     CDS             4515..>5149
                     /locus_tag="KJL94_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4412124.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1173 c         1225 g         1376 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccct
//
LOCUS       DAEBXD010000198         5141 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEBXD010000198 DAEBXD010000000
VERSION     DAEBXD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.199"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..253
                     /locus_tag="KJL94_003986"
                     /pseudo
     CDS             1..253
                     /locus_tag="KJL94_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1489
                     /locus_tag="KJL94_003987"
     CDS             746..1489
                     /locus_tag="KJL94_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD4412125.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERVM
                     DRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1504..1926
                     /locus_tag="KJL94_003988"
     CDS             1504..1926
                     /locus_tag="KJL94_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD4412126.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1984..2340
                     /locus_tag="KJL94_003989"
     CDS             1984..2340
                     /locus_tag="KJL94_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412127.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2433..2651
                     /locus_tag="KJL94_003990"
     CDS             2433..2651
                     /locus_tag="KJL94_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBD4412128.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2653..3018
                     /locus_tag="KJL94_003991"
     CDS             2653..3018
                     /locus_tag="KJL94_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD4412129.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            3015..3680
                     /locus_tag="KJL94_003992"
     CDS             3015..3680
                     /locus_tag="KJL94_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412130.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3680..4045
                     /locus_tag="KJL94_003993"
     CDS             3680..4045
                     /locus_tag="KJL94_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBD4412131.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4500..4655
                     /gene="hokD"
                     /locus_tag="KJL94_003994"
     CDS             4500..4655
                     /gene="hokD"
                     /locus_tag="KJL94_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4412132.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1406 a         1089 c         1413 g         1233 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
     1381 tctcggcgag cgggtgatgg atcgcatggt catgaacggc gggcgctggg ttaattttaa
     1441 ctgggagagc tggcgtccga atgttagcca ttcgagggtt gttaagtagt ttcaggagga
     1501 tttatggcga aaccttttac tcccgaacag cgggaagaac tgaagacgcg aattgtggaa
     1561 ctcgtgcatc aggacggtcg ggtcacgatt cggcagttgt ccgatgaaac aggtatcagt
     1621 cgtgcgtctg tcggtcgctt atgcatagaa ctggtcgcaa gtggtgatgt atataattct
     1681 ggctacggct tattcccgtc tgaacaggct cgcaaggact ggcaaagcgc ccgcaaaaaa
     1741 ctctcgagag taaaggtgag gaaaccggtt gttgttgatc cggaccttat ctggtcatta
     1801 cctgacggag aaatacgccg ctacgacagg cgcctgaata taatctgtcg cgagtgccgg
     1861 aagagcgaag ctatgcagcg tgtactggct ttctatcagg gtaattttca ggaggcgata
     1921 ctgtgaatga aattagctat caggcttcaa ttaccgctgg cattcgcatc aaaggagagg
     1981 agcatggaaa taaaaccaga agatgagtta agcaatatcg ttttatttcc gataaaagag
     2041 gatgaccctc gtaatcaggt taattttctt tataagccat cggaaagacc atattgccat
     2101 cacgcctctg tacgggttga cgaaaaagag cgtcaggtcc gctgtaaaat ctgcggtgcg
     2161 gttgtggagc cgtttgactg gatgctctct gtggcaaaaa gagaaaccag actggcagat
     2221 gatgtaaggc tattgcgcca ggaggaacag gaaaggcgga gaaatataga aaagctgata
     2281 cagattgagc gtaacgcgaa agcgcggata cgcagggcga caaaatccag aactgaataa
     2341 ttaaatttag cactgttaaa aatttaatcc ttaaccggag ggatttctgc accctcaaat
     2401 catcaggaga ccgcccgaaa gggcggtaat gaatggttac attatttaga aaaaaatatc
     2461 cgcgaaagag tagaacaaca gaattcctgt ttctcattct gtttatcgtg ttgatgacac
     2521 cgatatcccc gcaaattttt gtctggataa tcggaaaaat aattgagcca gttattgaat
     2581 tgtataacga cgtggtatgg gcgtcattca acacactgca caataaaatt aatccgtata
     2641 aggaaagctg atatggcact gacgaaaaaa caacgtgcag aactgcgcat gaagttcggt
     2701 ggtcgctgcg cttattgcgg ctgcgaactt ggcgaaaagt ggcatgcaga ccatgtaaaa
     2761 ccggtcattc gttttgatgg aaatatgctt caccaggaac gtgacgatat atccaacatg
     2821 gtaccagaat gccacccatg caatctgcac aagcattgca gtagcctgga agattatcga
     2881 cgaattatca gtgatggtcg tcgtgaattc cttgcgtccg ggaaaggcaa agcgctggtt
     2941 cgtatgggat tggttgaaat gaaatctgac ccggttgtgt tctggtttga aaaatatcaa
     3001 gaaggggcta cggcatgact actattacca aagagcgact gcaatggctg gctaacattt
     3061 ctggccgcga tgatattgac gatatagacg gcggtgaaat tcgtgaactg gcgcttatcg
     3121 ctctggcatc actggaagca gagcctgtag cggaatgcat tgttgaagat gggggcatgt
     3181 gtattgacgg gttcggtgag tatgtgggtc actcgctgcc tgatggaatg catgagcttt
     3241 atgctgcccc gcgaatgcga caacctgatg gatggaaagc cgtagctgta gcgtggaagg
     3301 tgacgtttac tcaagttgac caggaatcta atacgttcac tgctatatat ttttacaaag
     3361 cggaagttga gcgttgggta cgactgcata aagcatgtga ttttcgggca gatataacac
     3421 cgctttacgc agcgccggca gtgccggttg cagtaaacga cgacatggct tacgcattcc
     3481 atcacgcact gtcagattca tcgcttggct ctgatgaaat cgaagaaatt aaaaccggtt
     3541 tgcgtgctgc ctttgccaac gtcactatcc aaccagagcc gatagtgccg gatgaaatcg
     3601 ggccaaacga tagtaatacg tttgattatg ttgatggctg gaacgcctgc cgcgctgcca
     3661 tgcttaaggg agataaataa tgattaatcg aaccaaactg gagcacattc tcgaatatgc
     3721 caggcagcag aaatgtattg ggcaactttg taaaattcca ccaggagata tggttgaaat
     3781 cgtggaaatg gccatgcgta aggctggcaa ctctccggta actctggctg gctggataag
     3841 ctgtagtgat gcagttcctg ctgaatattg cgatgtgatt cttctcgatg atctcgggaa
     3901 tgtattcccc ggttcctggg ataaggtttt ttgccccact cgtggcggga ataagatggc
     3961 ttttgtggac aaagacggcg ttgaagtaga gagctcaact cactggatgc cgctaccgga
     4021 accaccgcag gaggtgaatc aatgacctgg cctgaggcat tcaccacggt aggaattgcg
     4081 atggcggtgg cgctggtggt gtattcgatt tgccgttggg gttaacaaac aaaaaccccg
     4141 gattgatggt ccggggtttt tgaaggaaac aaaacagaaa cagcaattgc cgttacctgt
     4201 tgttaccatg gcaagtaaac gtatctcagg cgagcgcatt gcgccgttct gacgcagata
     4261 aattagcctg gatagaaggt gctggcaata aaaaatagcg ttttcttatc ggtatcggta
     4321 agattgctgc gggtgcttga ggctgtctgc ctcgggcatg ccactgtaag gcagacagag
     4381 aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct gaggcccaat
     4441 ctatgtctca caaatgtagg ttagcctctt acgtgccgaa aggcaaggag aagcaggcta
     4501 tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga
     4561 cggcactggt aacgaggaaa gacctctgcg aggtacgaat ccgaaccggc cagacggagg
     4621 tcgctgtctt cacagcttac gaacctgagg agtaagagac caggcggggg agaaatccct
     4681 cgccacctct catgtgtcag gcatcctcaa cgcacccgca ctaaacccgc ttcggcgggt
     4741 tttttgttgc gtgctgaata cgcagggtga aaaataacca tatatttgat tatatacaca
     4801 acaaaaaata aaagtcattg tacttgcaca ttaaacaatc aaatatacgg cgtgaaataa
     4861 atatttatca gattaatatt tttgtctcta tgtggatata accgtttgta cttataaacc
     4921 tgggggcatc gtggaaaaaa taaagaaact atttagtagc aaatacgcag tcatacgtcg
     4981 tgatgacctg tcagttatag tcgaaatgga ttacttccct gaaaccccaa aatcaataat
     5041 gtatcgtaat ggtcgaaagg caattttttt accgatgagg gtaagtgaca ttatgggaaa
     5101 tgataaactg ctggatgaat tgcgagtcag agcatcctgt t
//
LOCUS       DAEBXD010000199         5075 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEBXD010000199 DAEBXD010000000
VERSION     DAEBXD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.200"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..180
                     /locus_tag="KJL94_003995"
     CDS             <1..180
                     /locus_tag="KJL94_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4412133.1"
                     /translation="RLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVEL
                     HDKVIGHYLNIKHYQ"
     gene            197..325
                     /locus_tag="KJL94_003996"
                     /pseudo
     CDS             197..325
                     /locus_tag="KJL94_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            452..1312
                     /locus_tag="KJL94_003997"
     CDS             452..1312
                     /locus_tag="KJL94_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBD4412134.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1522..1926
                     /locus_tag="KJL94_003998"
                     /pseudo
     CDS             1522..1926
                     /locus_tag="KJL94_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2033..2869)
                     /locus_tag="KJL94_003999"
     CDS             complement(2033..2869)
                     /locus_tag="KJL94_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD4412135.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2869..3672)
                     /locus_tag="KJL94_004000"
                     /note="aph(3'')-Ib"
     CDS             complement(2869..3672)
                     /locus_tag="KJL94_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBD4412136.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(3733..4548)
                     /gene="sul2"
                     /locus_tag="KJL94_004001"
     CDS             complement(3733..4548)
                     /gene="sul2"
                     /locus_tag="KJL94_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBD4412137.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4878..5054
                     /locus_tag="KJL94_004002"
     CDS             4878..5054
                     /locus_tag="KJL94_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD4412138.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
BASE COUNT         1263 a         1321 c         1364 g         1127 t
ORIGIN      
        1 cgcctgaagg gaaagctgca cgtaatcagc aagcgttaca ctcagcgcat tgagcgacat
       61 aacctgaatc tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa
      121 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcagtaa
      181 gttggagtca ttaccgaaga cggcttacgc caccatcaaa ggtattgagg tgatgcgtgc
      241 actacgcaaa ggccaggcct cagcatttta ttatggtgat cccctgggcg aaatgcgcct
      301 ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct
      361 aactttgcaa cagtgcctac attcaaatat gtatccgctc atgagacaat aaccctggta
      421 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacattttc gtgtcgccct
      481 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      541 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      601 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      661 taaagttctg ctatgtggtg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
      721 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      781 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      841 cactgctgcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      901 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      961 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     1021 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1081 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1141 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1201 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1261 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1321 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1381 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1441 ccactgagcg tcagacccct aaaagaactt tccgctaagc gatagactgt atgtaaacac
     1501 agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg
     1561 gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac
     1621 gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat
     1681 gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttctacgg
     1741 gttcgctgcg agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa gcgtcgcggt
     1801 ttctgcccga gctgtggggc gcggcggatg gccgaaagtg ccgccttgct ggttgatgaa
     1861 gtactgcctg aacaacccat gcgtcagtgg gtgttgagct tcccgtttca gctgcgtttc
     1921 ctgtttgggg tcgtttgcgg gaaggggcgg aatcctacgc taaggctttg gccagcgata
     1981 ttctccggtg agattgatgt gttcccaggg gataggagaa gtcgcttgat atctagtatg
     2041 acgtctgtcg cacctgcttg atcgcggccg cgatagctag atcgcgttgc tcctcttctc
     2101 catccgcgtt ccaagctgcg gaaaggcacc cataagcgta cgcctggtcg agcaggcgac
     2161 gcggatcgac gtccagcgca cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat
     2221 cgagacaaag gtcgtctctg tcagccggat cgtagaacat attggcggcg ccaaagccca
     2281 cttcaccgac cagaccgacg ggatctatca ccagccagcc gcgactggag aacatgatgt
     2341 tttcatgatg cagatcgcca tgtagcccac gcagttccga ggcattgctc atcatttgat
     2401 cggctataat cgccgcgtgg acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg
     2461 cccgctgaaa caaagctgca aagcgatccc ggatcgggag aagggcagaa ggcaggggtt
     2521 cctcagatgc ggcatacagc ttcgccatta gttccgctgc aatttcggtc gcctggtagt
     2581 cgccgtgctc ggcaacgatg tgagagagca ttcgctcccc ggcatattcg agcaacatca
     2641 gattgttctc acgaccgagc aaccggactg ctcccctccc attgcgccat accagatagt
     2701 cggccccgcg cagttcatca gcaatgtctt ctataggttt caatcccttg acgattgcag
     2761 gagtcccgtc tggcaatgaa actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa
     2821 caggttgcga aacgtgccaa tgagcaggaa aaacaggcgg catgaacatc aaccccaagt
     2881 cagagggtcc aatcgcagat agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa
     2941 tacattgaat aggacagcga aggcgcgctc tgcttcatct ggcgctgccc agttctcttc
     3001 ggcgttagca atcatgagtg ccaaatcggc atagcgatct gctgttccga gccgcccaag
     3061 gtcgatcaga cccgtgcatt gaagagtttt agggtccacc atgaagttcg gcatgcaggg
     3121 atcaccatgg caaacaacca tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg
     3181 ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc
     3241 gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct
     3301 gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat
     3361 tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat
     3421 tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca
     3481 agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg
     3541 gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc
     3601 accagattct ccgcctctga caggcaacca gtcagaatgc gattcaccaa aaaaaatatt
     3661 agttcgattc aatggaggtt ccttcagttt tctgatgaag cgcgaatata gagaaatatc
     3721 ccgaatgtgc agttaacgaa ttcttgcggt ttctttcagc gccgccaata ccgccagccc
     3781 gtcgcgcaag gggcgcggct cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc
     3841 aagctctgca gcgagtgtcg cggccccgac atcccccgga ccacggcctg tgagcgcgcg
     3901 cagaaaggat ttgcgcgaaa cagacagaag caccggcaaa tcgaagcgca gccgcaattc
     3961 atcgaaccgc gccagcaccg agagcgaggt ttcgggagca gcccccagaa aaaaccccat
     4021 gccgggatca aggacaaggc ggttgcgttt gataccggca cccgtcagcg ccgcgatgcg
     4081 cgcgtcaaag aacgccgcaa tgtgatccat gatgtcgcca gcgggtgcct cgcgccgatc
     4141 tgcctgcccg tcttgcaccg aatgcataac gacgagtttg gcagatgatt tcgccaattg
     4201 cggatagaac gcagcgtctg gaaaaccgcg aatatcattg agataggcca caccacgcga
     4261 caaggcatag gcttgcgtcg cgggttgata actgtcgagc gagacgggaa tgccatctgc
     4321 cttgagcgcg tccagcaccg gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc
     4381 ggcgtcggga ttgctggatg ccggaccgag gtcgatcaca tctgccccct cggccatcag
     4441 cttacgcgcc tgcgcaatgg ctgcgtctgg cgccagatac cggcctccat cggagaaact
     4501 gtccgaggtt atgttgacga tgccgaaaat gatgagcgat ttattcatgg gggcttctat
     4561 aataataata atcgagcatg agtctcatac ggatgctcgg gtcgaaaggg aatccccagg
     4621 cgagtaacct gtttgcggtg ctccattagc tgcaggagca gaatagcata catctggaag
     4681 caaagccagg aaagcggcct atggagctgt gcggcagcgc tcagtaggca atttttcaaa
     4741 atattgttaa gccttttctg agcatggtat ttttcatggt attaccaatt agcaggaaaa
     4801 taagccattg aatataaaag ataaaaatgt cttgtttaca atagagtggg tatcggagac
     4861 gaagaaggac aaggtacatg ctgaccgata ccaagctgcg caatctcaag cccagggaca
     4921 aactctacaa agtgaatgac cgggaaggtc tctatgtggg ggtcgcctca gaaaacggaa
     4981 tctatggtca ctcccgtttt tgcaacaccg attttgacga caagttggct tgcttgaatc
     5041 tatccggcgt ctgaatggga ttttattccc gcgcc
//
LOCUS       DAEBXD010000200         5023 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEBXD010000200 DAEBXD010000000
VERSION     DAEBXD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.201"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="KJL94_004003"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="KJL94_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBD4412139.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="KJL94_004004"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="KJL94_004004"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBD4412140.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="KJL94_004005"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="KJL94_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBD4412141.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="KJL94_004006"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="KJL94_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBD4412142.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGIMLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="KJL94_004007"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="KJL94_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBD4412143.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1208 c         1255 g         1415 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc atgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DAEBXD010000201         4973 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEBXD010000201 DAEBXD010000000
VERSION     DAEBXD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.202"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="KJL94_004008"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="KJL94_004008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBD4412144.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="KJL94_004009"
     CDS             1020..1703
                     /locus_tag="KJL94_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBD4412145.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="KJL94_004010"
     CDS             1700..2161
                     /locus_tag="KJL94_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBD4412146.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="KJL94_004011"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="KJL94_004011"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD4412147.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="KJL94_004012"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="KJL94_004012"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBD4412148.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KJL94_004013"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KJL94_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBD4412149.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KJL94_004014"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KJL94_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBD4412150.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1303 c         1240 g         1255 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAEBXD010000202         4926 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEBXD010000202 DAEBXD010000000
VERSION     DAEBXD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.203"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            92..244
                     /locus_tag="KJL94_004015"
                     /pseudo
     CDS             92..244
                     /locus_tag="KJL94_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1625..2572
                     /gene="icsP"
                     /locus_tag="KJL94_004016"
     CDS             1625..2572
                     /gene="icsP"
                     /locus_tag="KJL94_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBD4412151.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3134..3506)
                     /locus_tag="KJL94_004017"
                     /pseudo
     CDS             complement(3134..3506)
                     /locus_tag="KJL94_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3750..3869
                     /locus_tag="KJL94_004018"
                     /pseudo
     CDS             3750..3869
                     /locus_tag="KJL94_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4119..>4926
                     /gene="ospB"
                     /locus_tag="KJL94_004019"
     CDS             4119..>4926
                     /gene="ospB"
                     /locus_tag="KJL94_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBD4412152.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPE"
BASE COUNT         1543 a          907 c          991 g         1485 t
ORIGIN      
        1 aaatacgttc ttaatattct tgatgggact aatcatttta atctctatac tcaaatataa
       61 acattaccat gaacaatata attgttccta agtggaatgt ctccacggag aacactttat
      121 ctatcgggaa atagtgcggg caacagtgtt taattatatc gagtgcaatt acaatcgctg
      181 gcgacggcac cgttggtgtg gcggcctcag tccggaacaa tttgaaaacc agaacctcgc
      241 ttaggcctgt gtccatatta cgtgggtagg ctcacagagc ctcacttacg actgcgtcca
      301 tattatgtgg gtaggatcaa taaagacgtt ttatatgtgt ctgcttgcag ccagaaaatt
      361 tataccctgc aggtatcata aacatgcttg ataacttaat tggggctccc tcttcctttt
      421 atggtgtaat tgtttgattg aatacttccg gggatttcag tatgaaatga agtatattta
      481 atatacttta caaaaagttt gtattagtgg aataaggaca atagcttatc caaactgcca
      541 agcctcttta ttataagtaa gatctggcac tggaatattc tcaaaaggac taacatcatc
      601 accacgacct tcaaccaacc attttattgc atttaaatcc cagaatagtt cagtagtggt
      661 caatttggga aacgcacaat attgtacggt tacgttatcg caatagagtg aactctcgcc
      721 ctatacggcg ttttcaagga ttagtcctta atcggacaac cacattaaag cataataaat
      781 aattaagctt taataatata ctatagatac agttctatta taaaaaatac tattttaggt
      841 gatgcattaa attagtagaa tatttattct tttataaaag caccaatgaa ttgtccaagt
      901 aaacatgaga taaaatgtgt agaggataac tgagtttggt cactttaact gtattagtcg
      961 cagtctgact ctcgacttta aaaggatggg aattacctgg actgatagtg agaaagcttt
     1021 ccattgaaat aagtcaaata aaagttgatg gcttcagtgc taaagtgagg tgcccgctag
     1081 ccgtattcac tcgaatcgga attcaatgta ttgcttctgc catttccata atatttcacg
     1141 ggaaaaacaa aatctgaaaa actatactta agtgttgtat tgagtcagtt gagatacaaa
     1201 tacatattta tcagcacacg ttacgtcaaa tacaggaggt tgtcatgttg gtactgaaag
     1261 gcacgttggc tattgcagcc gtgatgacaa tttattgtct tatcgttgtt cttatggaac
     1321 gcttttctga ttgatttcat attggcgaga gaacaagaag gtgagtagaa ttgtcgcgga
     1381 atcctgaaac tatcagcctc aggtatgaac accaaaaggt aggtagagaa atctatggcc
     1441 cccgttactt tattcgcaca atgaggtatt tttttatttt tacaaaagtt caataaatat
     1501 tataataaaa gatatgttct tggtaacata aacaaatatt taattaaaat aatgcttggc
     1561 attgacgttt atcttataaa gtaagaagat catggacatt tcaaccaaaa aagtagagtt
     1621 ctcgatgaaa ttaaaattct ttgtacttgc actttgtgta cctgcgatct ttactacaca
     1681 tgctaccact aactatccac ttttcatacc ggacaacatc agtacggata ttagtctagg
     1741 atctctgagt ggcaaaacaa aagaacgcgt ttatcatccc aaggaagggg ggagaaaaat
     1801 tagtcaactg gactggaaat acagtaatgc gactattgtt agaggtggca tcgattggaa
     1861 gctaattcca aaagtgtctt tcggagtttc cggttggact actttaggta accagaaagc
     1921 aagcatggtt gataaagact ggaacaattc caatacccct caggtatgga ccgaccagag
     1981 ctggcatccc aatacgcatc tccgtgatgc taacgaattc gagctgaatc ttaaaggttg
     2041 gttattaaat aatttggatt atcgacttgg actaatagca ggttaccaag agagtcgtta
     2101 cagttttaat gcaatgggag ggagttatat ttatagtgaa aacgggggga gcaggaataa
     2161 aaaaggggca catcctagtg gtgaaagaac aataggttac aaacagctct tcaaaatacc
     2221 ttatattgga ttaactgcta attaccgcca tgagaatttt gagtttggag cagaactgaa
     2281 atatagtggt tgggttcttt catctgatac agataaacac tatcagactg agacaatttt
     2341 taaagatgaa ataaaaaacc aaaattactg ctctgttgct gcgaatattg gatactatgt
     2401 cacccccagt gcaaaatttt atatagaagg ctccagaaat tacatttcta ataaaaaagg
     2461 tgatacatct ctttatgagc aaagtaccaa tatatctggc accattaaaa atagtgcaag
     2521 tattgaatat attggttttc tcacttccgc aggtataaag tatatttttt gaattggttc
     2581 accgagttga ttacgttttc atggctaaaa attgccgccc attgtgagtc atattcgccc
     2641 tgactttcca gaaccatacg aactgcccgt tgacggactt cgggggaaaa acgtgtattt
     2701 ttagtcattc tgtttacctc tttctcaggg cgtttagtct ccaggatttc cggggcggtt
     2761 caaaaaactc ttcgttgatt actgtggact tactgttggc attaccgacc ctgagaccgg
     2821 agaaataaga accgcttaga tcatcgtggc tgttctcggg gtatcaagtt acacatgggc
     2881 agaggctacc caaggtctac tattatcgcc tgaaagcgct gatggaacag tgctatcagg
     2941 gacatgctga tgaaaatacc taaataatca gccagcactc tatctttcca aatccacagc
     3001 atagcaaaga gagcaaaaga gcctgtaaat tcagaaatta aggaaacgcc tcttggtgta
     3061 cataatctat cttatgttaa atgaaatcta atttcttgat tatatgctgg atcgttcgtg
     3121 ggtggctttg cccctatatt tccagacacc tgttatcatt ggcccgctgc cgcagatatt
     3181 cccgtggcga gcgataatcc agcgcactat gcggatgcca ttcgttataa tgctcgaatg
     3241 cctctgcaag gttctttgct gccgttaacc cgtcaggttt gggcatgata ctgatgtagt
     3301 catactttat tgttttcacg aagctctttg ctattctgtt actctccgga ctccgcaccg
     3361 ccgtgttctt cggttcaaca aagtcccaac atccgggtga actggtgtgt ttcattagct
     3421 cggtagcatg aaccattatc cgtcagtcac ttcactggag acgccggaag ctcgttgccg
     3481 aagcggcgtt ccaccgctcc cagcatgtcg ccaacgggag agcatctacg gtctgattga
     3541 actgtttatt caaattgtcc accgtctgtc agtacgctct gaacgtcggc tggttaaaac
     3601 actgctcgct gattttcaga gggttcacgg taaaacagca cttttattcc ggattgcgga
     3661 agcggctctg aataatccgg atgggctggt taaagaggtt gtgtaccatc tgcatattgt
     3721 tgaaccagat cgcagcggta agagaagttc atcatacttg gcgcagttac gcgatgtttc
     3781 tgctagaggt gatgccgtaa agaacggcag aacattaccg gaacaagata gcggtctacc
     3841 tgcactggta tcagacccgg ggcttccccg gatgatctcc acggtacttt aggtactgtg
     3901 tctatattat gtaggtagga tcaattaact ttgtagttga aattctttca ttgaaataat
     3961 gaataaaaaa gacaaaccgc attaaacaat gccagaatat aattgcattt ttgttccgtt
     4021 tttttaaatc atagtataca aagaagcttg catactatat gaaaatataa caccctttac
     4081 tatgatgttt ataaaacaat atatggagtc atgtaggtat gaatttagat ggtgttagac
     4141 catactgtag aatagtcaat aaaaagaatg aaagcatatc agatattgca tttgcacata
     4201 taataaaaag ggtaaaaaat tcatcatgta ctcacccaaa agcagcattg gtttttttag
     4261 gagagaaagg tttttgtgat agcaatgatg ttctatctat tatgggacaa caaataccaa
     4321 gagtatttaa gaacaagatg ttatatgatt atgtttttaa aaatgaaaaa agtaaaaatg
     4381 attttctaaa aatggctgaa tcatggctac cacagagtga accaatagta ataaataatg
     4441 atgatgacgc attgaatgct gctgcttatt tttctgtaaa aaaagcgaaa ataaaaacag
     4501 taaacgatac tgattttaaa gagtataata aggtttatat tcttgggcac ggtagtcctg
     4561 gttctcatca attaggcctt ggttcggaac ttattgatgt acaaacaatc atttcaagaa
     4621 tgaaagactg tggtattcta aatgtgaaag atatccgttt tacttcatgc ggctccgctg
     4681 ataaagtggc tcctaaaaat tttaacaatg cccctgctga aagtctttct tgtatcctta
     4741 actctctgcc tttttttaag gaaaaagaat ctttgctaga gcagataaaa aaacaccttg
     4801 aaaacgatga gtcattgagt gatggtctaa aaatatccgg ctatcatgga tatggagttc
     4861 actatggtca agagcttttt ccctactcac attatcgttc aacttcaatt cctgctgatc
     4921 cggagc
//
LOCUS       DAEBXD010000203         4871 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEBXD010000203 DAEBXD010000000
VERSION     DAEBXD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.204"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..527
                     /locus_tag="KJL94_004020"
     CDS             <1..527
                     /locus_tag="KJL94_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBD4412153.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="KJL94_004021"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="KJL94_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBD4412154.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="KJL94_004022"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="KJL94_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD4412155.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="KJL94_004023"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="KJL94_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBD4412156.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="KJL94_004024"
     CDS             4170..4343
                     /locus_tag="KJL94_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412157.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="KJL94_004025"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="KJL94_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBD4412158.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4665..4805)
                     /locus_tag="KJL94_004026"
                     /pseudo
     CDS             complement(4665..4805)
                     /locus_tag="KJL94_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1296 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaac acgaggcata
     4681 taggtctgat tccgatattc atttggagtc agacctttta atttcaggct aattcttctg
     4741 ctgttgtagt attcaatata ttccgtaaca gcatccttca gttcgcttat attactgaac
     4801 tcattagact ggccccctga atctccagac aaccagtatc acttatttaa gtgatagtct
     4861 taatactagt t
//
LOCUS       DAEBXD010000204         4638 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEBXD010000204 DAEBXD010000000
VERSION     DAEBXD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.206"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..311
                     /locus_tag="KJL94_004027"
     CDS             <1..311
                     /locus_tag="KJL94_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD4412159.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KJL94_004028"
     CDS             468..1217
                     /locus_tag="KJL94_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBD4412160.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KJL94_004029"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KJL94_004029"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBD4412161.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KJL94_004030"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KJL94_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBD4412162.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KJL94_004031"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KJL94_004031"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBD4412163.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KJL94_004032"
     CDS             4084..4557
                     /locus_tag="KJL94_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBD4412164.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1163 c         1138 g         1099 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaattc
//
LOCUS       DAEBXD010000205         4610 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEBXD010000205 DAEBXD010000000
VERSION     DAEBXD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.207"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="KJL94_004033"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="KJL94_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="KJL94_004034"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="KJL94_004034"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBD4412165.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4153..4610
                     /gene="pgaB"
                     /locus_tag="KJL94_004035"
                     /pseudo
     CDS             4153..4610
                     /gene="pgaB"
                     /locus_tag="KJL94_004035"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1317 a         1033 c         1061 g         1199 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct ttaaaagtcg tgcgttaact
     4201 gacttcactt tagaacttag tgcgcgcgta aaagccattc gcggtccaca tattaaaact
     4261 gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg gtttgcacag
     4321 aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat gccttatatg
     4381 gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac aattgaccaa tcaaattaaa
     4441 aacatccctc aggctaaaga caaatctatt ttagaattac aggcacaaaa ctggcagaaa
     4501 aatggtcagc atcaggctat tccttcgcaa caactcgctc actggatgag cctattacaa
     4561 ctgaatggag tgaaaaacta tggttattat cccgacaatt ttctgcataa
//
LOCUS       DAEBXD010000206         4601 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEBXD010000206 DAEBXD010000000
VERSION     DAEBXD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.208"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..395
                     /locus_tag="KJL94_004036"
     CDS             <1..395
                     /locus_tag="KJL94_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412166.1"
                     /translation="LEYATTRKKSEVVYSGVSVTIPTAPTNLVSLLKTLTPSSGSLAP
                     FFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQLTFSGSVPDTLVSSRNAATT
                     TDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            395..850
                     /locus_tag="KJL94_004037"
     CDS             395..850
                     /locus_tag="KJL94_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBD4412167.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            853..1461
                     /locus_tag="KJL94_004038"
     CDS             853..1461
                     /locus_tag="KJL94_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBD4412168.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            1471..2802
                     /locus_tag="KJL94_004039"
     CDS             1471..2802
                     /locus_tag="KJL94_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBD4412169.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            2803..4533
                     /locus_tag="KJL94_004040"
     CDS             2803..4533
                     /locus_tag="KJL94_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBD4412170.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         1261 a         1135 c         1204 g         1001 t
ORIGIN      
        1 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
       61 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
      121 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
      181 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
      241 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
      301 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
      361 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
      421 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
      481 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
      541 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
      601 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
      661 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
      721 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
      781 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
      841 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
      901 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
      961 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     1021 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     1081 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     1141 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     1201 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     1261 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     1321 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     1381 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     1441 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     1501 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     1561 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     1621 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     1681 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     1741 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     1801 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     1861 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     1921 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     1981 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     2041 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     2101 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     2161 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     2221 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     2281 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     2341 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     2401 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     2461 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     2521 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     2581 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     2641 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     2701 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     2761 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     2821 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     2881 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     2941 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     3001 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     3061 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     3121 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     3181 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     3241 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     3301 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     3361 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     3421 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     3481 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     3541 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     3601 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     3661 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     3721 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     3781 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     3841 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     3901 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     3961 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     4021 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     4081 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     4141 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     4201 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     4261 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     4321 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     4381 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     4441 ccatcaacaa ggtcaatgct caaacaggtg gcagaagatc ttggcgggat gaaaaacctt
     4501 gaccctattg aaaagagagt tgaggtagcc tgagtttaac ggacactcct tcctgaaata
     4561 gaatggcatc agaaggagct aataatgagc agaaaaaccc a
//
LOCUS       DAEBXD010000207         4556 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEBXD010000207 DAEBXD010000000
VERSION     DAEBXD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.209"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            291..851
                     /locus_tag="KJL94_004041"
     CDS             291..851
                     /locus_tag="KJL94_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBD4412171.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            794..1183
                     /locus_tag="KJL94_004042"
                     /pseudo
     CDS             794..1183
                     /locus_tag="KJL94_004042"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1163..1720
                     /locus_tag="KJL94_004043"
                     /pseudo
     CDS             1163..1720
                     /locus_tag="KJL94_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1818..3427
                     /locus_tag="KJL94_004044"
                     /pseudo
     CDS             1818..3427
                     /locus_tag="KJL94_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3528..4019)
                     /locus_tag="KJL94_004045"
     CDS             complement(3528..4019)
                     /locus_tag="KJL94_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HBD4412172.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4024..4200)
                     /locus_tag="KJL94_004046"
     CDS             complement(4024..4200)
                     /locus_tag="KJL94_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4412173.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1398 a          728 c          901 g         1529 t
ORIGIN      
        1 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa attccgatgc
       61 atgatttcgg atttttcgaa agtcctgatg aaaggctgcg ctacggcact cacggattta
      121 attgttattc ctaatgtatc tatgaattaa tgttttataa ggatttttta taaccattta
      181 taagtatttt caagcctggc ttgttgcaaa caatgtataa agcacttagg caataataat
      241 tacattcagc aactatcatc atcggtattg tttgtgggcg gaatttcata ttgacaacag
      301 tacagttctt atatctatta ataatagaaa gagatctaca acctacagat tggtgtagct
      361 ttatggaaaa agactatttg agaattagta gtactgtatt agtgagctta ttgtttgggc
      421 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
      481 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      541 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      601 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      661 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      721 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      781 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      841 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      901 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      961 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
     1021 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
     1081 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
     1141 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
     1201 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
     1261 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
     1321 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
     1381 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1441 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1501 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1561 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1621 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1681 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1741 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1801 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1861 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1921 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1981 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     2041 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     2101 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     2161 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     2221 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     2281 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     2341 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     2401 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2461 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2521 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2581 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2641 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2701 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2761 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2821 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2881 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2941 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     3001 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     3061 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     3121 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     3181 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     3241 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     3301 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     3361 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3421 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3481 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3541 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3601 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3661 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3721 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3781 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3841 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3901 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3961 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     4021 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     4081 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     4141 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     4201 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     4261 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     4321 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     4381 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4441 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4501 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgata
//
LOCUS       DAEBXD010000208         4525 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEBXD010000208 DAEBXD010000000
VERSION     DAEBXD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.210"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..289)
                     /locus_tag="KJL94_004047"
     CDS             complement(<1..289)
                     /locus_tag="KJL94_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412174.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAY"
     gene            343..784
                     /gene="tnpA"
                     /locus_tag="KJL94_004048"
                     /pseudo
     CDS             343..784
                     /gene="tnpA"
                     /locus_tag="KJL94_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(902..1846)
                     /gene="hypT"
                     /locus_tag="KJL94_004049"
                     /gene_synonym="qseD"
     CDS             complement(902..1846)
                     /gene="hypT"
                     /locus_tag="KJL94_004049"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBD4412175.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            2191..2910
                     /locus_tag="KJL94_004050"
     CDS             2191..2910
                     /locus_tag="KJL94_004050"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBD4412176.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2976..4274
                     /locus_tag="KJL94_004051"
     CDS             2976..4274
                     /locus_tag="KJL94_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBD4412177.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1129 a         1003 c         1116 g         1277 t
ORIGIN      
        1 aataagcgga agcctggcga aggatatcgt tactgcggcg cagttcacga ttttcacgtt
       61 ccagctcttt cagacgctga cgttcagcgg tggtgagccc tccatcaccg cccccggtat
      121 cccgctcatg ctggcgaacc cagacacgca gagtctccgg cgtacagcca atctttggag
      181 caatggaaca aattgtcgcc cattgtgagt catattcgct ctgactttcc agaaccatac
      241 ggactgcccg ttgacggact tcgggggaaa aacgagtatt tttagtcatc ctgtttacct
      301 ctttctcagg aagtttagtc tccaggattc ccggggcggt tcatagcgca cacccgatgg
      361 aactgtaaat atcacatagt tttttgcgcc aaaataccga agacaggtgt tctacagaga
      421 gaagcgtaga gcaacaggca gtattttgag aaagctgtgt gagtggaaaa gcgtacggat
      481 tctggaagct gaatgctgtg cagatcatat ccatatgctt gtggagatcc cgcccaaaat
      541 gagcgtatcc ggctttatgg gatatctgaa agggaaaagc agtctgatgc tttacgagca
      601 gtttggtgat ttgaaattca aatacaggaa cagggagttc tggtgcaggg gggactacgt
      661 cgatacagtg ggtaagaaca cggcgaagat acaggattac ataaagcacc agcttgaaga
      721 ggataaaatg ggagagcagt tatcgattcc ctgtccgggc agcccgttta cggaccgtaa
      781 gtaacgaagt tggatgcaaa tgtcagatcg tgtgcgcctg ttagggcgcg gctggtaaga
      841 gagccttata ggcgcatttg aaaaacctcc ggctatgccg gaggatattt attgcacgtc
      901 gtcaattgcg gcgtgctggc tcttccattg atatctgctg gattaaatcg tctggcatat
      961 gttcctgtaa aatacgccag aggttctcgg cggttctatt cagtcgggtg ttcattcggt
     1021 aaatataagc ctgaatgggg actgtcaatt tagcagcatc aaggcatatc agacgtttgg
     1081 tctgtagttc atcgacgatc gaccagatcg gtaaccaggc aatgccgtat ccgttgagcg
     1141 ccatattctt taataactcg ctcatcgaag agataaatat cgtgcgcccg gtaatacctc
     1201 caacctctgc cagatgacga ttgaccaacc tgcccatata agatgtggcg gtatagttga
     1261 gcaaaggaac ctggggctga gtgatatcga atacagggtg cccctgtgcg tcggcggcac
     1321 ataccgggta caactcagat tcaaacagtt tcaggcagca aaaaggcgat tgcataagat
     1381 cttcatcgcg aaacgaaata ataaagtcac tcttgccttc gcgtaacgta tttacggcct
     1441 ggacaacatc aatggcttca acatgataga caaattctcc gccataggcg gtaagtgaat
     1501 gcaccagttt gggtaatacg ctcagggata gtgagtgcgc ggctgcaatt ttaatgttgg
     1561 gaacgccgag taaactctgg ccgtttaact cccccaaatt gcattcaagc tgctgtaata
     1621 agctgcgagt ctgggaatga aagagcttgc cctcttcggt taattgtaaa ggggtggtcg
     1681 tgcgatcgaa taaaacaacg ccgatcgctg cctccagcgc cttaattcgt cggctgaatg
     1741 cgggttgcga aagattacgc tcttttgccg cctgagaaaa atggcgacag gcttccagtg
     1801 tcaggaaatc gtataaccat tttgtctcga tattcttcat taccatggca cctgtatttt
     1861 caatccaaag acataataaa acatcccggc aataaaaata atgttctcaa ttagcacaac
     1921 ctggccttta tcattcgttt tgtgcataac cccttcattt ccggtattga cgttgatatc
     1981 acatttcgcc aaaaaaacga aatttttccg tcagataacc agaatacatc atcactatgg
     2041 tgaataagcg gaatgttcca tagctgcatc atgcgtgtct tgcatcgcct tgtacaacct
     2101 tgcatttctc atcgcccatt tcgactgtta aaaaagagag cagcagttat ttaccgcgta
     2161 ttgtcattaa cgttttgaga ggcaggtatt gtgaaaggtt taattggtgt actcggtgga
     2221 atggggccgg cagctaccgt cgacctgttt aataaatttg taacttttac cgctgcacaa
     2281 tgtgatcagg aacatattcc tttaattatt tcttcgatcc cggatattcc tgaccgtacc
     2341 gatgcattaa tgcatcatgg gcgttcacca ttacccgtta tgcgtgatta tatgcataag
     2401 ctggaagatg ccggggcaga atgtattgtt attccctgta ataccgcgca ttactggttc
     2461 aaagaattaa aagatgcttg tcatattgat atattaagta tcgtagaaac tacgatcaat
     2521 gaagttcgcg cctgtggtaa aacccgaatt ggcttgttag ccaccaatgc cacgctatat
     2581 atgggtttat accaaaaagg aattgagtcg ctgggattta cctgtgtcag ccccgatgcc
     2641 gacggtcaga aaaaagtgat ggaaagtatt tattccttga aagctggcaa tatagcgcac
     2701 gcacaaaagt tgatgaatga gcaggctgaa atattatttt cccgcggtgc agaaatcctt
     2761 gttcttggtt gtacagaaat tccggttatt ctggcgcaag ccgttaaaga gcagccttcc
     2821 cgctatattg actcaacggc gtcactcgtt cgtgccggaa taaaatggta cgaaaatcgt
     2881 gtcggtaaac attatctttt aacccaataa tcatttaaat cgcagccaga aagttattcg
     2941 cttttaactg aattatattt ataacggaga acattatggt ttggctggaa attatcgtag
     3001 tacttggtgc aatatttttt ggtattcgcc aggggggaat cggtattggt ttatgtggcg
     3061 ggcttgggct tgccattctg actctgggat ttggtctgcc tatggggtcg ccgccagtcg
     3121 acgtgatcct gattattatg accgttgttg tggcggcctc agccttacag gccgcaggcg
     3181 gaatggatta tctggtacgc gtcgctggta attttatgcg gcgtaacccg aaatatatca
     3241 acataatcgc gccgattatc acctggtcta tgaccattat ggccggtact gggtttattg
     3301 ttttttccac gctgccggtg attgctgaag tggcgaaaga gtccggtatc cgtccttccc
     3361 gcacgcttgc cggttcggtt gtcgcttcgc aggttgcgat ttccggttcg ccgatcagcg
     3421 ccgcaatggc ggctatgctc actattatgg aggtaaacgg tgtcagtttt attcaagtga
     3481 tgtcggtatg tcttcccacc tcttttgctg ccgcgatggt cgccgctttt attgcttccc
     3541 gacagggatg tgagttgcag gatgacgagg tctaccttga gcgcctgcaa aaaggactgg
     3601 tgcaaaaata cgagaataat agcagcatca agccaggtgc gacgctctcc gtcggattgt
     3661 tcctgctggc gacggtcgct attgttattc ttgctgcctt cccccaactg cgtccgggtt
     3721 ttgatatcgg taaaccgatg gaaacacgcg atatcattat tatctgtatg ctctctgccg
     3781 cctgcctgat ggtggtgctg tgtaagacgg cgaccgatgc cattattctc acctccacat
     3841 tccgtgcggg gatgagttcg ctggcggtaa ttctcggcat tgtaacctta ggcaccactt
     3901 ttattgatgc gcatttaact gagattaaag atattgctgg tgatattttg caggcgtatc
     3961 cgatgttgtt ggcgctggtt ctttttggca cttgcgcgct gctctactcg caaggtgcga
     4021 ccacgccgtt aattatcccg cttgccgtcg cgcttgacgt gcccacctgg gcaattctgg
     4081 cgtcctatgt ggccgtcacc ggagtatttg tactgccaac gtatccaaca tcactggctg
     4141 ccatggaatt tgatactacc ggcacaactc gtgtaggcaa atatgtgctg aatcatccat
     4201 ttatgctgcc tgggttgggg ggaattattg ctggagtggc attaggcttt gtgattgcgc
     4261 caatgattat ttagcactat caaacatcag tatcgatggt ttatacacaa taaagacaga
     4321 tatggcggaa tatagtctgt tgtaagcgcc aacaaagaat aggggaattc cgccatgtct
     4381 gaacatcttc acaatatggt attaagtgtt gtcgtttaat gatttatttc ttggttatgt
     4441 tgtgtattag ctgcatctat cgctttttga tacctgaggt gtactggcaa tagcggacac
     4501 taccatttgt tcttttttta agcag
//
LOCUS       DAEBXD010000209         4395 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEBXD010000209 DAEBXD010000000
VERSION     DAEBXD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.211"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            110..967
                     /gene="focA"
                     /locus_tag="KJL94_004052"
     CDS             110..967
                     /gene="focA"
                     /locus_tag="KJL94_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBD4412178.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1022..3304
                     /gene="pflB"
                     /locus_tag="KJL94_004053"
     CDS             1022..3304
                     /gene="pflB"
                     /locus_tag="KJL94_004053"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD4412179.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3496..4236
                     /gene="pflA"
                     /locus_tag="KJL94_004054"
     CDS             3496..4236
                     /gene="pflA"
                     /locus_tag="KJL94_004054"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBD4412180.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         1111 a         1096 c         1076 g         1112 t
ORIGIN      
        1 ccatgcgagt tacgggccta taagccaggc gagatatgat ctatatcaat ttctcatcta
       61 taatgctttg ttagtatctc gtcgccgact taataaagag agagttagtg tgaaagctga
      121 caaccctttt gatcttttac ttcctgctgc aatggccaaa gtggccgaag aggcgggtgt
      181 ctataaagca acgaaacatc cgcttaagac tttctatctg gcgattaccg ccggtgtttt
      241 catctcaatc gcattcgtct tctatatcac agcaaccact ggcacaggca caatgccctt
      301 cggcatggca aaactggttg gcggcatttg cttctctctg gggctgattc tttgtgttgt
      361 ctgcggagcc gatctcttta cttccacagt gttgattgtt gttgctaagg cgagtgggcg
      421 catcacctgg tgtcagttgg ctaaaaactg gctaaatgtc tattttggca acctggtcgg
      481 cgcactgctg tttgtacttt taatgtggct ttccggcgag tatatgaccg caaatggtca
      541 atggggacta aacgtcctac aaaccgccga ccacaaagtg caccatactt ttattgaggc
      601 cgtctgtctt ggtatcctgg caaacctgat ggtatgtctg gcagtatgga tgagttattc
      661 tggccgcagc ctgatggaca aagcgttcat tatggtgctg ccggtcgcga tgtttgttgc
      721 cagcggtttt gagcacagta tcgcaaacat gtttatgatc ccgatgggta ttgtaatccg
      781 cgacttcgca tctccggaat tctggaccgc tgtcggttct gcaccggaaa atttttctca
      841 cctgaccgtg atgaacttca tcactgataa cctgatcccg gttacgatcg gtaatattat
      901 cggcggtggt ttgttggttg ggttgacata ctgggtcatt tacctgcgtg aaaacgatca
      961 ccattaatgg ttgtcgaagt acgcagtaaa taaaaaatcc acttaagaag gtaggtgtta
     1021 catgtccgag cttaatgaaa agttagccac agcctgggaa ggttttacca aaggtgactg
     1081 gcagaatgaa gtaaacgtcc gtgacttcat tcagaaaaac tacacgccgt acgagggtga
     1141 cgagtccttc ctggctggcg ctactgaagc gaccaccacc ctgtgggaca aagtaatgga
     1201 aggtgttaaa ctggaaaacc gcactcacgc gccagttgat tttgacaccg ctgttgcttc
     1261 caccatcacc tctcacgacg ctggctacat caataaagcg ttggaaaaag ttgttggtct
     1321 gcagactgaa gctccgctga aacgtgctct tatcccgttc ggtggtatca aaatgatcga
     1381 aggttcctgc aaagcgtaca accgcgaact ggacccgatg atcaaaaaaa tcttcactga
     1441 ataccgtaaa actcacaacc agggcgtgtt cgacgtttac actccggaca tcctgcgttg
     1501 ccgtaaatcc ggtgttctga ccggtctgcc agatgcttat ggccgtggcc gtatcatcgg
     1561 tgactaccgt cgcgttgcgc tgtacggtat cgactacctg atgaaagaca aatacgctca
     1621 gttcacctct ctacaggctg atctggaaaa cggcgtaaac ctggaacaga ctatccgtct
     1681 gcgcgaagaa atcgctgaac agcaccgcgc tctgggtcag atgaaagaaa tggctgcgaa
     1741 atacggctac gacatctctg gtccggctac caacgctcag gaagctatcc agtggactta
     1801 cttcggctac ctggctgctg ttaagtctca gaacggtgct gcaatgtcct tcggtcgtac
     1861 ctccaccttc ctggatgtgt acatcgaacg tgacctgaaa gctggcaaga tcaccgaaca
     1921 agaagcgcag gaaatggttg accacctggt catgaaactg cgtatggttc gcttcctgcg
     1981 tactccggaa tacgatgaac tgttctctgg cgacccaatc tgggcaaccg aatctatcgg
     2041 tggtatgggc ctcgatggtc gtaccctggt taccaaaaac agcttccgtt tcctgaacac
     2101 cctgtacact atggggccgt ctccggaacc gaacatgacc attctgtggt ctgaaaaact
     2161 gccgctgaac ttcaagaaat tcgccgctaa agtgtccatc gacacctctt ctctgcagta
     2221 tgagaacgat gacctgatgc gtccggactt caacaacgat gactacgcta tcgcttgctg
     2281 cgtaagcccg atgatcgttg gtaaacaaat gcagttcttc ggtgcgcgtg caaacctggc
     2341 gaaaaccatg ctgtacgcaa tcaacggcgg cgttgacgaa aaactgaaaa tgcaggttgg
     2401 tccgaagtct gaaccgatca aaggcgatct cctgagctac gatgaagtga tggagcgcat
     2461 ggatcacttc atggactggc tggctaaaca gtacatcact gcactgaaca tcatccacta
     2521 catgcacgac aagtacagct acgaagcctc tctgatggcg ctgcacgacc gtgacgttat
     2581 ccgcaccatg gcgtgtggta tcgctggtct gtccgttgct gctgactccc tgtctgcaat
     2641 caaatatgcg aaagttaaac cgattcgtga cgaagacggt ctggctatcg acttcgaaat
     2701 cgaaggcgaa tacccgcagt ttggtaacaa tgatccgcgt gtagatgacc tggctgttga
     2761 cctggtagaa cgtttcatga agaaaattca gaaactgcac acctaccgtg acgctatccc
     2821 gactcagtct gttctgacca tcacttctaa cgttgtgtat ggtaagaaaa ctggtaacac
     2881 cccagacggt cgtcgtgctg gcgcgccgtt cggaccgggt gctaacccga tgcacggtcg
     2941 tgaccagaaa ggtgctgtag cgtctctgac ttccgttgct aaactgccgt ttgcttacgc
     3001 taaagatggt atctcctaca ccttctctat cgttccgaac gcactgggta aagacgacga
     3061 agttcgtaag accaacctgg ctggtctgat ggatggttac ttccaccacg aagcatccat
     3121 cgaaggtggt cagcacctga acgttaacgt gatgaaccgt gaaatgctgc tcgacgcgat
     3181 ggaaaacccg gaaaaatatc cgcagctgac catccgtgta tctggctacg cagtacgttt
     3241 caactcgctg actaaagaac agcagcagga cgttattact cgtaccttca ctcaatctat
     3301 gtaattagat ttgactgaaa tcgtacagta aaaagcgtac aataaaggct ccacgaaagt
     3361 ggggcctttt ttagcacgag agcctttttt gtcagctatc tatactttaa ggtgactgcc
     3421 aaaacagact cgacgtagcc ttcgagctgc gcaccaacac ggcctcagat gggccacatc
     3481 tggagaaaca ccgcaatgtc agttattggt cgcattcact cctttgaatc ctgtggaacc
     3541 gtagacggcc caggtattcg ctttatcacc tttttccagg gctgcctgat gcgctgcctg
     3601 tattgtcata accgcgacac ctgggacacg catggcggta aagaagttac cgttgaagat
     3661 ttgatgaagg aagtggtgac ctatcgccac tttatgaacg cttccggcgg cggcgttacc
     3721 gcatccggcg gtgaagcaat cctgcaagct gagtttgttc gtgactggtt ccgcgcctgt
     3781 aaaaaagaag gcattcatac ctgtctggat accaacggtt ttgttcgtcg ttacgatccg
     3841 gtgattgatg aactgctgga agtaaccgac ctggtaatgc tcgatctcaa acagatgaac
     3901 gacgagatcc accaaaatct ggttggagtt tccaaccacc gcacgctgga gttcgctaaa
     3961 tatctggcga acaaaaatgt gaaggtgtgg atccgctacg ttgttgtccc aggctggtct
     4021 gacgatgacg attcggcaca tcgcctcggt gaatttaccc gtgatatggg taacgttgag
     4081 aaaatcgaac ttctccccta ccacgaactg ggcaaacaca aatgggtggc gatgggtgaa
     4141 gaatacaaac tcgatggtgt taaaccaccg aagaaagaga ccatggaacg cgtgaaaggc
     4201 attcttgagc agtacggtca taaggtaatg ttctaatttc ctcgtaaagc ggcaacttta
     4261 ttgagttgcc gcttttttat tccgcgcttc attctgctac agcgttttta acacatctta
     4321 tagtgcatct accatatccc gtacctgaga taataacgac tgataatagc tcgcgtgaag
     4381 ttcttcccca tcgaa
//
LOCUS       DAEBXD010000210         4381 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEBXD010000210 DAEBXD010000000
VERSION     DAEBXD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.212"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            148..1179
                     /locus_tag="KJL94_004055"
                     /pseudo
     CDS             148..1179
                     /locus_tag="KJL94_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1266..1742)
                     /locus_tag="KJL94_004056"
     CDS             complement(1266..1742)
                     /locus_tag="KJL94_004056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412181.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(1735..2499)
                     /locus_tag="KJL94_004057"
     CDS             complement(1735..2499)
                     /locus_tag="KJL94_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBD4412182.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            2745..3506
                     /locus_tag="KJL94_004058"
     CDS             2745..3506
                     /locus_tag="KJL94_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD4412183.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(3613..4113)
                     /locus_tag="KJL94_004059"
     CDS             complement(3613..4113)
                     /locus_tag="KJL94_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412184.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT         1326 a          907 c          806 g         1342 t
ORIGIN      
        1 ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat gcatttgtct
       61 ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca catttcaata
      121 ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct tttcaaatta
      181 atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct ggaaagttca
      241 gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg cattattcac
      301 taccgcattt attatcattt cgcaactcac gcgcggcgtt aatacgtttt acaatacatt
      361 gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt gggcactgtt
      421 agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt ttggcgttgg
      481 tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc tgctcattcc
      541 caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt cttcattaat
      601 cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca tttggtctat
      661 tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt aaccgcaatc
      721 tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa tacgttctca
      781 tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt cacggtgctg
      841 caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc cggcattatt
      901 ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct actggcactg
      961 gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt atttacctgt
     1021 ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga gctggactac
     1081 tttattccat ctcgggttct ggttggatta ggcgcagtat gttttacatt gttctctatt
     1141 gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa tcccagcgtt
     1201 attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa tataaaatta
     1261 taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa aatatagaca
     1321 ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc gagaaaattg
     1381 agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc cttagcatta
     1441 cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg ggaaaatttc
     1501 ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc atcttttttt
     1561 aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc atttcttgtt
     1621 aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca gagagcaaaa
     1681 aaacacacct gtagcaatgt gaaaacaagt attaatataa aatacatttt cttattagtc
     1741 atataatagc acctctgaac aaattgcata agtttcgtca ttagaggatt ttatacatct
     1801 tgataaatac cgctgccctt ccccaagcaa ataatgcata ctatttacat catatataaa
     1861 atttgcacct ggcacacagg gtaaatgctt tttcgaaatt tcagcggcct ctatttgaga
     1921 tttagcgagg ccaatctcgt taagattctc tgaaagatat aaatcacagg tgtcaatctt
     1981 acctgattta accaatggat aatccgttat actgtaggta tctttaaaag ccacggcaaa
     2041 tatttcaata ataacaatta ctaccacaag cgaaatcaaa atgcgaaaaa gaggaatact
     2101 ccttttgcgc tctacttttt caacaacatc ttcttgtaaa tgagaatcat cataagattg
     2161 tttacttatt gttatgtttt cactgatgta aaagcctact ttgggtatcg taagaatcac
     2221 attttcttca acaccaatat ttttaaaact tttgcgtaac agggatatat attggttcaa
     2281 tgtgttgttt gacagatcaa atgaaaatct ctccaaaacg ctatcaatga ttgagttcct
     2341 gtcaaccgct ccaccattac tttccagtaa cactgccaaa atacaccccg ccgtagctgg
     2401 caaatttatc tcatcctggg tatttacatt cgagagcgag catttgttcg gatcaaaaat
     2461 aatcgcgtca ttgataatga aaaaacatct ccgctccatg caggcaacaa cacaaaataa
     2521 gattttgtca ttttagccta ttcctggtgg aactgacaca agaaactaaa atacaaatat
     2581 aaccctgaaa aagtcatata aaacttatat ccacagaata aaaaatcaaa catgtcagtt
     2641 ttatcaatat aaagtatcgc aaggcgaaaa gtgatgcaag aatactccta agtaaatgaa
     2701 agcgcaccac atgaaattat caacacatgt aatttcatca ttaggtgcta tcgaaaataa
     2761 ctcaggagct acttaagatg aataatgtct tactcatgag acccgatgac tcagaattta
     2821 tgatacctga tgactggtca gggctaaacg caaaaggcat tacatacgaa caagataata
     2881 gagtgacaga gtcttttctg ccttttaaat ctttttattg ttcaccggtg tgcacgacag
     2941 ttgaactcat ctcacaaaat aattgttttg ctatgggtat ccgctacctt ctggaaaaga
     3001 aaagagactt aaggctaaat cactttaaag ccttctctca taaatgcgag acaaaacctg
     3061 acgtgttaat tatgaccggg tgcaccgtcg ctgatactta tagtatattc tcacgcattg
     3121 ttgaagaaaa tataatgaaa acgatgacac ctatcgtcat cattactggg catcactcag
     3181 aagatctttg ttctctggca tcgaaactgg gaggagttaa aataatatca ccgacagaaa
     3241 agattgcaaa aatagaaaac gctatcaatg attcttttaa cagcaagcct aaaaaaataa
     3301 gatttttaac agatatactc tctcctcaac aaattgagac catgaaaatg gttgcgcagg
     3361 gtagatccgt taatgagatt tcagccgcaa tgaatattag catcaagacc gtgtatgcac
     3421 ataaagttcg tgctatcaat aagctgggaa ttaagaaaaa agtttatgaa gcgctttttt
     3481 ataaagcgct aattcagttc aactgatgta aaaatcaccc tcgcttaata aataatatat
     3541 tctatgtctt tttttataag ttaaaaaagg cgggatatca aaccccgcca tgaaattaac
     3601 taatcatctg cattattctg cgtagatata agcctgggta cggatggtgt aagagtctgg
     3661 ctcaacaacc tgttctttca tatgggaaac gacgcgatag gcaccacctg ttccagtatt
     3721 tttcagcatc atataacgct taccgccaat ctcttcggag gcagctgaag gaggatcctg
     3781 ctcaagcgtt gtcagggaaa ctttcagttt atggtccggg ttattgtcac cagaaattac
     3841 tgcatccgct gaagtactgc tctccgcata aatatcatcc gggaaagtta cagcatagtc
     3901 gtggctctgc ccatcagatg cgccaacaat acctgttgcc aaaggcgtcc atgcagttaa
     3961 cgtaccgcta tatacccctt tcactggttg aatggtatta ttaacaacga taggcttggt
     4021 caaagtaaga ttagctgacg cttcagtacc ctggcttact aaagttcccg cctgagaagc
     4081 aaaagctgtc cccgccagca tgattgctaa caatgttttc ttcattatat tttctccatg
     4141 attttcattt acactgcaaa atgcagtttc tttctttgca tttacgtgct atcgcgaaaa
     4201 tgcttccacc aaataatgga aacatcttca caaaaataaa ttatgagttt cttaatttag
     4261 aaactataaa catgtcgccg ctatctggta taaccctgat gcacttttct tcgcaggaag
     4321 ttggtatttt gcgatacagg attgatcttt agaataaccc catttcacat taaactcgcc
     4381 t
//
LOCUS       DAEBXD010000211         4354 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEBXD010000211 DAEBXD010000000
VERSION     DAEBXD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.213"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(129..875)
                     /gene="cutC"
                     /locus_tag="KJL94_004060"
     CDS             complement(129..875)
                     /gene="cutC"
                     /locus_tag="KJL94_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBD4412185.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(889..1455)
                     /locus_tag="KJL94_004061"
     CDS             complement(889..1455)
                     /locus_tag="KJL94_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD4412186.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1671..3404
                     /gene="argS"
                     /locus_tag="KJL94_004062"
     CDS             1671..3404
                     /gene="argS"
                     /locus_tag="KJL94_004062"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBD4412187.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3581..4069
                     /locus_tag="KJL94_004063"
     CDS             3581..4069
                     /locus_tag="KJL94_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBD4412188.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4187..4255)
                     /locus_tag="KJL94_004064"
                     /pseudo
     CDS             complement(4187..4255)
                     /locus_tag="KJL94_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         1140 a         1079 c         1065 g         1070 t
ORIGIN      
        1 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
       61 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      121 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      181 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
      241 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
      301 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
      361 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
      421 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
      481 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
      541 gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc gagaacgccc
      601 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
      661 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
      721 atcgtcaccc gctggcgcac ggatttcagt acccccaacg acggcgttaa gcccccctct
      781 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
      841 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
      901 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
      961 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     1021 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     1081 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     1141 acgtttttcc ccgggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     1201 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     1261 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     1321 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     1381 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     1441 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     1501 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     1561 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     1621 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     1681 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     1741 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     1801 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     1861 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     1921 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     1981 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
     2041 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac acagcagtgc
     2101 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
     2161 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
     2221 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
     2281 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
     2341 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacttacg
     2401 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
     2461 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
     2521 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
     2581 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
     2641 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
     2701 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
     2761 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
     2821 cgggtggtac cgtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
     2881 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
     2941 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
     3001 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
     3061 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
     3121 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
     3181 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
     3241 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
     3301 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
     3361 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
     3421 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
     3481 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
     3541 cccaggaaaa cgataacggt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
     3601 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
     3661 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
     3721 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
     3781 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
     3841 tactgaacaa cttaacttgc ttaaaaaatc acagcgagcc tggctggatt ttagggacaa
     3901 agaatgtgaa ttaatccttt caaatgagga tgttcaggat ttaagtgacc cctattctga
     3961 atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
     4021 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatcttt
     4081 ttggcatgtt ttgttgcaag ctattcctga taaatgattg caacaaaaca tccagccttt
     4141 tgactgagtt attaaacata ctcgcgagcg cgtaattttt tgtctttcag cgataattca
     4201 caatcacttc attccgttgt acctttagcg gtggaattaa ccgccctgaa ccgccccggg
     4261 tttcctggag agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag
     4321 cataataagc tttttctgct tctgccggag gagt
//
LOCUS       DAEBXD010000212         4323 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEBXD010000212 DAEBXD010000000
VERSION     DAEBXD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.214"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..157)
                     /locus_tag="KJL94_004065"
     CDS             complement(<1..157)
                     /locus_tag="KJL94_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD4412189.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(307..468)
                     /locus_tag="KJL94_004066"
     CDS             complement(307..468)
                     /locus_tag="KJL94_004066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412190.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(465..653)
                     /locus_tag="KJL94_004067"
     CDS             complement(465..653)
                     /locus_tag="KJL94_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBD4412191.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            913..1248
                     /gene="iraM"
                     /locus_tag="KJL94_004068"
     CDS             913..1248
                     /gene="iraM"
                     /locus_tag="KJL94_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBD4412192.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1529..1637)
                     /locus_tag="KJL94_004069"
                     /pseudo
     CDS             complement(1529..1637)
                     /locus_tag="KJL94_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2160..2236)
                     /locus_tag="KJL94_004070"
     tRNA            complement(2160..2236)
                     /locus_tag="KJL94_004070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Arg,seq:tct)
     gene            complement(2338..2413)
                     /locus_tag="KJL94_004071"
     tRNA            complement(2338..2413)
                     /locus_tag="KJL94_004071"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2378..2380),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="KJL94_004072"
     CDS             complement(2558..3379)
                     /locus_tag="KJL94_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD4412193.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="KJL94_004073"
     CDS             complement(3394..3750)
                     /locus_tag="KJL94_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD4412194.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4323)
                     /locus_tag="KJL94_004074"
     CDS             complement(3763..>4323)
                     /locus_tag="KJL94_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD4412195.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1187 a         1178 c          856 g         1102 t
ORIGIN      
        1 gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc cactgagacg
       61 gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct gacgtggtgt
      121 atgacacacc tgttgtgatt ttttccatct ggtccatacc ccgtctcccg ttatccggaa
      181 gctgacaaca ataaaaaagc caccagttaa ctactgatgg ctctgataac tcatgcaagc
      241 gtctcagacg acccactgac actaccggtg agtttaacga taccttccat ttgactggct
      301 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      361 atacgttgca tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact
      421 gtcttttatg gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt
      481 gcaccactga ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
      541 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
      601 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      661 tccaacatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      721 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      781 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      841 ctatggttta attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa
      901 caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata
      961 tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc
     1021 ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg
     1081 ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa
     1141 aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt
     1201 tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca
     1261 catctgacaa aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt
     1321 gccattccat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
     1381 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
     1441 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta
     1501 aaaatttctc ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg
     1561 ggaaagcaac aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc
     1621 ggctgctgca atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat
     1681 aatccagata tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct
     1741 ggctattctg ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc
     1801 atcgcaaacc ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg
     1861 atcaggagta cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac
     1921 tatatcaata cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc
     1981 cacaccatat gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata
     2041 ctcaatcaat acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat
     2101 cctgagccag gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat
     2161 ggcgcgctgt actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg
     2221 gctgagctaa caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac
     2281 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     2341 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     2401 gagctaaagg gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccacc aggataattc
     4321 agc
//
LOCUS       DAEBXD010000213         4307 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEBXD010000213 DAEBXD010000000
VERSION     DAEBXD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.215"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            437..2089
                     /locus_tag="KJL94_004075"
     CDS             437..2089
                     /locus_tag="KJL94_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD4412196.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDPNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="KJL94_004076"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="KJL94_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="KJL94_004077"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="KJL94_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="KJL94_004078"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="KJL94_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(3809..4009)
                     /locus_tag="KJL94_004079"
                     /pseudo
     CDS             complement(3809..4009)
                     /locus_tag="KJL94_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         1175 a          984 c          979 g         1169 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccctaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gagcagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaaagaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgtaccat ttttcagcgt tccccagaag ctttccatcg
     3841 gagcgttgtc gtaacagtta cctttacgcg acattgatgt tttcagacca aactgctcct
     3901 gtatgacccg gtaatcgtat gcgcagtact gtgaacctcg atcggagtgg tggattagcc
     3961 cggcaggtgg gcgctggctc ctgagcacca taaacagggc tttacccgta aatattggct
     4021 ccttctgatg ccattctatt tcaggaagga gtgtccgtta aactcaggct acctcaaaaa
     4081 gttatcggtg cgtttatcga aaaacatatg ttctactaat tggagtcatt gtctgtaaaa
     4141 gcaactcaaa aatacaagtt gcttattccc cttgttggta agaacttaat tctaactgtt
     4201 tgttctgggt gttttaaacg tcctcctttt tatgcttgtt cataacggat atttaatagg
     4261 taacctttgt tttagcatct tctgatttat ttaaattatg tgatagc
//
LOCUS       DAEBXD010000214         4297 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEBXD010000214 DAEBXD010000000
VERSION     DAEBXD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.216"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            389..3697
                     /gene="icsA"
                     /locus_tag="KJL94_004080"
                     /gene_synonym="virG"
     CDS             389..3697
                     /gene="icsA"
                     /locus_tag="KJL94_004080"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBD4412197.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGIIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3883..4297
                     /locus_tag="KJL94_004081"
                     /pseudo
     CDS             3883..4297
                     /locus_tag="KJL94_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1299 a          742 c         1018 g         1238 t
ORIGIN      
        1 ttggagagtc tggtaggaaa gttgtgatat gaatacattc atgttattgg ctttggggat
       61 taacatcaag gggcagatag aaccattggg tgtatagaaa aacaaagtgg ttaaactttg
      121 ggcaagcgta cagaaagaac tgaaaagttg cggtctgaag cagactatca tagtaaaatt
      181 attaatttat tatcgaacat atagctttcc ccctcttttt ttcaaagcaa gacacaggta
      241 aatttctccc gttgcattga tatataacac agctctcatg ttttggttga ggctttgttt
      301 aatatgtttt tgcatatatc gtccctttat tccggataaa acggaatctt ttcaggggtt
      361 tatcaaccac ttactgataa tatagtgcat gaatcaaatt cacaaatttt tttgtaatat
      421 gacccaatgt tcacaggggg gggccggaga attacctacg gtaaaggaaa aaacatgcaa
      481 attgtctttt tctccttttg ttgttggtgc atccctgttg ctcggggggc caatagcttt
      541 tgctactcct ctttcgggta ctcaagaact tcatttttca gaggacaatt atgaaaaatt
      601 attaacacct gttgatggac tttctccctt gggagctggt gaagatggaa tggatgcgtg
      661 gtatataact tcttccaacc cctctcatgc atctagaact aagctacgga ttaactctga
      721 tattatgatt agcgcaggtc atggtggtgc tggtgataat aatgatggta atagttgtgg
      781 cggtaatggt ggtgactcta ttaccggatc tgacttgtct ataatcaatc aaggcatgat
      841 tcttggtggt agcggcggta gcggtgctga ccataacggt gatggtggtg aggctgttac
      901 aggagacaat ctgtttataa taaatggaga aattatttca ggtggacatg gtggcgatag
      961 ttatagtgat agtgatgggg ggaatggagg tgatgccgtc acaggagtca atctacccat
     1021 aatcaacaaa gggactattt ccggtggtaa tggaggtaac aattatggtg agggtgatgg
     1081 cggtaatgga ggtgatgcca tcacaggaag cagcctctct gtaatcaata agggcacgtt
     1141 cgctggaggc aacggaggtg ctgcttacgg ttatggttat gatggctacg gtggtaatgc
     1201 tatcacagga gataacctgt ctgtaatcaa caatggagct attttaggcg gtaatggtgg
     1261 acattggggg gatgctataa atggtagcaa tatgaccatt gctaatagcg gatatataat
     1321 ttcaggtaaa gaagatgatg gaacacaaaa tgtagcaggt aatgctatcc acatcactgg
     1381 tggaaacaat tcattaatac tccatgaagg ttctgtcatt actggtgatg tacaggttaa
     1441 caattcatcc attctgaaaa ttatcaacaa tgattacact gggaccacac caactattga
     1501 aggtgattta tgtgctggtg attgtacaac tgtttcacta tcaggtaaca aattcactgt
     1561 ttcaggtgac gtttcttttg gtgagaacag ttctttaaat ttagctggaa tcagtagtct
     1621 ggaagcttct ggaaatatgt catttggcaa caatgtaaaa gtggaggcta ttataaataa
     1681 ctgggcgcag aaggactata aactgctaag tgcagataaa gggataacag gtttcagtgt
     1741 ttctaatata tctatcatca atccgttact cactactggt gctattgact atacaaaaag
     1801 ctatatcagt gaccagaata aattgatcta cggtttgagc tggaatgata cagatggcga
     1861 cagtcatgga gagttcaatc tgaaagaaaa cgctgaactt actgttagta ctattctggc
     1921 agataatctc agccatcata atataaatag ctgggacgga aaatccctaa caaaatcagg
     1981 ggagggaact ctcattttgg cggaaaaaaa tacctactct ggtttcacca acatcaatgc
     2041 aggcattcta aaaatgggga cagttgaagc tatgacacgt accgctggtg ttattgttaa
     2101 taaaggtgct accttgaatt tttcaggcat gaaccaaact gttaacactt tattaaatag
     2161 tgggactgtg ctaatcaata atattaatgc cccttttttg cctgaccccg tcattgtcac
     2221 aggtaacatg actctggaga aaaacggtca tgttattctc aataatagtt cgtcaaatgt
     2281 cggtcagacc tatgttcaga aaggtaattg gcatggaaag ggcggaatat tatctttggg
     2341 cgcggttctc ggcaatgaca acagtaaaac tgaccggctg gaaattgcag gccatgcgtc
     2401 tggtattacc tatgttgcag tgacaaatga gggaggctct ggagataaaa ctcttgaagg
     2461 tgttcaaatt atttcgacag attcttctga taagaatgct tttattcaga aaggccgtat
     2521 tgttgctggt agttatgact atcgcctgaa acagggcact gtatctggac tgaataccaa
     2581 taagtggtat ctaactagtc agatggataa tcaagaatca aaacagatga gcaatcaaga
     2641 gtctactcaa atgagtagtc gccgagctag ttcacagctt gtatcttcac ttaatttggg
     2701 tgaaggtagt attcacacat ggcgccctga agctggcagt tatattgcta acctgatagc
     2761 aatgaacacg atgtttagtc cttctctcta tgaccgacac ggtagcacta ttgttgatcc
     2821 tactacaggt cagctcagcg aaaccaccat gtggattcgt actgttggtg gacataatga
     2881 gcataattta gctgatagac aattaaaaac cacagctaac aggatggttt atcagattgg
     2941 tggagatatt ttgaagacaa acttcactga tcatgatggc ttgcatgtgg gtattatggg
     3001 agcttatgga tatcaggata gcaaaactca taataagtat actagttata gttcacgagg
     3061 aactgtgagc ggttatactg ccggtttgta cagttcttgg tttcaggatg aaaaagaacg
     3121 aacaggtcta tatatggatg cttggttgca gtacagttgg tttaataata cagtcaaagg
     3181 agatgggtta actggtgaga aatattccag caaaggaata acaggagctt tggaagctgg
     3241 ctatatctac ccaaccatac gctggactgc tcataataat attgacaacg cattgtatct
     3301 caatccacaa gtccagataa ctaggcatgg ggtaaaagca aacgactata ttgaacacaa
     3361 tggcactatg gtcacatcct ctgggggcaa taatattcaa gcaaaattgg gattgcgtac
     3421 atccttaatt agtcagagtt gtatcgataa ggagactctt cgtaagttcg aaccattttt
     3481 ggaagtgaat tggaaatgga gctcaaagca atatggtata attatgaatg gcatgtcaaa
     3541 tcaccagata ggcaaccgta atgtgattga actcaaaact ggtgtggggg ggcgtcttgc
     3601 agataaccta agcatctggg gaaacgtatc tcagcaattg ggtaataaca gttacagaga
     3661 cacccaaggt attttgggtg tgaaatatac cttctgataa taaaaatgca gggcgtgttg
     3721 atgtcctgca tttctctggg acccaccgtg taaaagctcg gtgggttttt gtgcacatga
     3781 tggtatgcct gcgatcaatg ccaaacgtgt ttaccagatc atgcgccaga atgcgctgtt
     3841 gcttggcgtt acacaggaat tgcattagaa agcgatgaac gaatgctacc gccaaatatt
     3901 cgtggttatg cgcctcagat tactggtatt gcagaaacta atgcacgtgt tgttgtatct
     3961 caacaaggtc gagtaatata tgactctact gtaccagctg gaactttttc tattcaggac
     4021 ttgagtagtt ctgtacgagg aatattggat gttgaaattt ttgagcaaaa tggtaaaaga
     4081 aaacatttcc aagtagaaat gtgcaggtgt gcctttctta tccagacttg gtcagagtag
     4141 gtataaatta gtaacaggtc tacctaaaac aaataataaa gcaactggtg atgcattttt
     4201 ttcggtgaag atcctacggg gtcctgagcc cggtgaagtg gcgaggcaaa tcacatgggg
     4261 aagcgtacag cctgaactga accgccccgg gaatcct
//
LOCUS       DAEBXD010000215         4237 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEBXD010000215 DAEBXD010000000
VERSION     DAEBXD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.217"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            134..2413
                     /gene="ydeP"
                     /locus_tag="KJL94_004082"
     CDS             134..2413
                     /gene="ydeP"
                     /locus_tag="KJL94_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBD4412198.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2661..2864
                     /gene="safA"
                     /locus_tag="KJL94_004083"
     CDS             2661..2864
                     /gene="safA"
                     /locus_tag="KJL94_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBD4412199.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            2932..3693
                     /gene="ydeO"
                     /locus_tag="KJL94_004084"
     CDS             2932..3693
                     /gene="ydeO"
                     /locus_tag="KJL94_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBD4412200.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4095..>4237
                     /locus_tag="KJL94_004085"
     CDS             4095..>4237
                     /locus_tag="KJL94_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD4412201.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1203 a          911 c          948 g         1175 t
ORIGIN      
        1 gataatagat taaaaataga aagagagtag tcccttactc ttatatatcc atgttggcga
       61 taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat tcatttccac
      121 acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgcag gtggttgggg
      181 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc atgatgttat
      241 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg catggccaga
      301 tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
      361 agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact
      421 tacctgggga gatcacgagc ttgaggctgc ggggcgtctc actcagcctt tgaaatatga
      481 tgccgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc
      541 acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc
      601 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc
      661 cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt
      721 aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg
      781 gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg
      841 gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc
      901 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta
      961 caacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
     1021 gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg aatttattca
     1081 aacgcatacc gtcggctttg acgagctacg ccgtgacgtt ctcaattccg agtggaaaga
     1141 tatcgaacgt atttctggac taagtcagac acaaatcgcc gaactggctg acgcatatgc
     1201 cgctgccgaa cgcaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
     1261 ccagaacgtg cagcaactgg tcaatttgct gttgatgaaa ggtaacattg gcaagcctgg
     1321 tgcgggtatc tgcccactac gtggtcactc taatgtacag ggcgaccgaa ccgtcggtat
     1381 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcccccc
     1441 acctcatgca cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc
     1501 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
     1561 cgctgtaccg ttaacgcaat tagatttggc ggtacacgta gccactaagc ttaaccgctc
     1621 tcatctgctg accgcacggc atagctatat tctgccggtt ctggggcgta gcgagattga
     1681 tatgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc
     1741 ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc
     1801 gggaatcgcg caggcagcac taccccagag cgtggtagcc tgggagtatc tggtggaaga
     1861 ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagttcgccg actataacca
     1921 gcgcatccgt catcccggtg gttttcacct gataaatgca gctgctgaaa ggcgctggat
     1981 gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc
     2041 agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac
     2101 gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat
     2161 gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc
     2221 gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta
     2281 ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaacact
     2341 tgataaccac gatccattaa gtggcattcc tggctataaa agtattccgg ttgaattaga
     2401 accatcaaat taatgcctct tctcatttct tctgctgtca tccgcacagc agaagaattc
     2461 ctcattgact attattgcgc aatttgctca catggattaa attaaactac atactataag
     2521 atataaactt ctgcctacag ctgtaagaaa ctccgctcag tactgaagca ccagtcctat
     2581 ttcttctttt ctccagcctg ttatattaag catactgatt aacgactttt aacgttatcc
     2641 gctaaataaa catatttgaa atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag
     2701 ataactataa agaaatcatg tctgcaattg tggttgtctt attactgaca cttacgttga
     2761 tagccatttt ttcggcaatt gatcagctga gtatttcaga aatgggtcgc attgcaagag
     2821 atcttacaca tttcattatc aatagtttgc aagactggaa ataaacagca aattataaat
     2881 gtgaaatgtt taaaaggtat cgataaaact ttattgtttt aaggagataa aatgtcgctc
     2941 gtttgttctg ttatatttat tcatcatgct ttcaacgcta acattttaga taaagattac
     3001 gccttctccg acggcgagat cctgatggta gataacgctg ttcgtacaca ttttgaacct
     3061 tatgagcggc attttaaaga aatcggattt aatgaaaata ccattaaaaa atatctacaa
     3121 tgcactaaca tccagacagt gacggtgcct gttcctgcga agtttttacg tgcttcaaat
     3181 gtaccgactg gattgcttaa tgaaatgatt gcttatctca actcggaaga acgcaatcat
     3241 cataattttt cagaactttt gcttttttct tgcctgtcta tttttgccgc atgcaaaggt
     3301 ttcattacac tattaactaa cggtgtgcta tccgtttccg ggaaagtgag aaatattgtc
     3361 aacatgaagc tggcgcaccc atggaagctg aaagatattt gtgactgcct gtacatcagt
     3421 gaaagcctgt tgaagaaaaa actaaagcaa gagcaaacga cattctcaca gattctatta
     3481 gatgccagaa tgcagcacgc aaaaaatttg atacgcgtag aaggttcagt caataaaatt
     3541 gccgaacaat gtggttatgc cagtacatct tattttattt atgcgttccg caaacatttc
     3601 ggcaacagtc cgaagagagt ttctaaggag taccgttgtc aaaggcacac gggtatgaat
     3661 acgggcaaca cgatgagtgc tttagctatt tgattatttg ctaacgagta gtcaaccacg
     3721 cacgctacgt aagaattata tggggcagcc attccctgcc ccgcgttgtt tttaggcgat
     3781 atatttattg aaataaataa gtgacatcca tcacatattt atgcacttac ataacctgtt
     3841 gcatgattat ttatgatctc aattcggcat tttgtcagta aaatgcaata atttattaaa
     3901 tatcaataaa ttagttgttt atcggcgaga aattacttaa tagaacagaa agtaatgtca
     3961 acgctttatg gactgttatt tccctttttt tagctaaatc tgctatctct ttatgtgact
     4021 aacttcactt acatccactt atttctcttc gtaaaattac ttcggaatta agtacaataa
     4081 gaagaggaac atttatgaag tctgcattaa agaaaagtgt cgtaagtacc tcgatatctt
     4141 tgatactggc atctggtatg gctgcatttg ctgctcatgc ggcagatgat gtaaagctga
     4201 aagcaaccaa aacaaacgtt gctttctcag actttac
//
LOCUS       DAEBXD010000216         4191 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEBXD010000216 DAEBXD010000000
VERSION     DAEBXD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.218"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..186)
                     /locus_tag="KJL94_004086"
     CDS             complement(<1..186)
                     /locus_tag="KJL94_004086"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBD4412202.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="KJL94_004087"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="KJL94_004087"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBD4412203.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="KJL94_004088"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="KJL94_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBD4412204.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="KJL94_004089"
     CDS             3863..4144
                     /locus_tag="KJL94_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBD4412205.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1018 a         1046 c         1062 g         1065 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta t
//
LOCUS       DAEBXD010000217         4170 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEBXD010000217 DAEBXD010000000
VERSION     DAEBXD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.219"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="KJL94_004090"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="KJL94_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBD4412206.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="KJL94_004091"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="KJL94_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="KJL94_004092"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="KJL94_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4170
                     /locus_tag="KJL94_004093"
     CDS             3787..>4170
                     /locus_tag="KJL94_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBD4412207.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFGH"
BASE COUNT         1038 a         1208 c         1023 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgccttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggcat
//
LOCUS       DAEBXD010000218         4155 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEBXD010000218 DAEBXD010000000
VERSION     DAEBXD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.220"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1010..1201)
                     /locus_tag="KJL94_004094"
     CDS             complement(1010..1201)
                     /locus_tag="KJL94_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD4412208.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1265..1588
                     /locus_tag="KJL94_004095"
     CDS             1265..1588
                     /locus_tag="KJL94_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBD4412209.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1578..1889
                     /locus_tag="KJL94_004096"
     CDS             1578..1889
                     /locus_tag="KJL94_004096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412210.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1864..2175
                     /locus_tag="KJL94_004097"
     CDS             1864..2175
                     /locus_tag="KJL94_004097"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBD4412211.1"
                     /translation="MHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLV
                     NHYYGLHDPNAPENRRTLTLPDNLPETKQAIAEGVTRGLPCRRDKRPSGCDSGSHRGR
                     D"
     gene            2256..2786
                     /locus_tag="KJL94_004098"
                     /pseudo
     CDS             2256..2786
                     /locus_tag="KJL94_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2793..3026
                     /locus_tag="KJL94_004099"
     CDS             2793..3026
                     /locus_tag="KJL94_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBD4412212.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3572..4039)
                     /locus_tag="KJL94_004100"
     CDS             complement(3572..4039)
                     /locus_tag="KJL94_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412213.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1003 a          947 c         1214 g          991 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacagc ttgtctgtga gcggatgccg ggagcagctt gtctgtgagc
      961 ggatgccggg agcagcttgt ctgtgagcgg atgccgggag cagcatatgt cagaggtttt
     1021 caccgtcatc cccgaaacgt gcgaggcagc tgcggtaaag ctcatcggcg tggtcgtgaa
     1081 acgattcaca gatatcagcc tgttcatccg cgtccagttc gttgagcttc tccagcagcg
     1141 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttagtca
     1201 ctgatgcctc cgtgtaaggg ggatttctgt tcaggaggta atgataccga tgaaacgcga
     1261 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     1321 gggtaaacag ctggcggtat ggatgcggcg ggtctgcctg ggggagccgg ttgcccgttc
     1381 cgggaaactg ccgacactgg caccgccgtt actgtgtcag ctggctgcca tcgggaataa
     1441 cctgaatcag acagcgagga aggtgaacag cgggcagtgg tcttccggta accgggttca
     1501 ggtggtggcc gcactgatgg ccatcgggga cgagctgcgc cggctgcgtc tggccgtcag
     1561 ggaacagggg acgcgggatg atagttaagt ttcatgcccg gggaaaaggt ggtggcagtg
     1621 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     1681 gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     1741 ccggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa aaagtgatgg
     1801 cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc atcctgtggg
     1861 tggatgcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta
     1921 cagaccggaa aacgcctcca gccgtactac gaccgcgcag acaggcctag aattgatgcc
     1981 tggcagacgc tggttaatca ctattacggg ctgcatgacc cgaacgcccc ggagaaccgc
     2041 aggacgctga cactccctga taatctgccg gaaacgaaac aggcgattgc tgagggcgtt
     2101 acacgcggtt taccatgccg gagagataaa aggccgtcag gatgtgattc aggctctcac
     2161 agaggcaggg attgacgtgg ttagggtgac gcgaagcagt atcagcattg cagacccgaa
     2221 cggcgggaag aatatcagac tgaaaggagc gttttatgag caatctttta cagacgggtg
     2281 cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg ctgaacgacg
     2341 agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa tctttaatca
     2401 ggaacgctat caacgaccat acatcagccc tgaaagaact tctggagagg tatcagaaaa
     2461 cgaccgtcga tacgatggac gcacactgga aaaccgtact gaaaatgagc gtgaaacgct
     2521 ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagc ctgctgtggt
     2581 accagggcat gaagataaac gcgaacatga atattctccg cgagtagaaa gagagtctgg
     2641 agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac ggacgttttc
     2701 tggtgttgcc cgaggggatg aaagccgaaa cgaactggac gatggaaaac gggaaacaga
     2761 atgccgtaag gctggtacag gagtaacaga gcatgacgga gctggaaaga catttgctga
     2821 acgcattaga acaactgcaa caggactata tgcagcggct gaacgaatgg gagagcgcct
     2881 tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
     2941 gggtcatgca gttgagtcag caggtgcagc acttgagcga gcagacagaa cgcttgagcc
     3001 agttatacag cgagaacagg agataaggga cgaacggctg atacaggaat gcgaacatgc
     3061 gttatccctg gagcgtgagc accagctgga aatacaggaa cggacactgg atggcccttc
     3121 gctgggatgg taatcagaga caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt
     3181 gggataatgg ttggttcggg ggcaccggga acgcgcttca gtcagacctt gctgaaaaca
     3241 taggaacgcg ttaaaacgca tacgggcgcg attctggtgt gtcttttgtt gctgtgtttt
     3301 gcctctattg ccagatcgcg ctcattcccc tgaacttctt ttttcccgct caggtccccg
     3361 tcttcttcgc ttccgaaagc atgaaaaaaa tccgtttcag ctgtctctgg ctggcaattc
     3421 ctgcgtgatt cacatggctg catagctatg catgtggtta aaatttaccg ggtgcgatcg
     3481 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgccga
     3541 acgcgtttca gcggtgcgta caattaagat attacgttaa attgtgaggg aatggtcccc
     3601 atatactgac tttatcatag ggatagggtt ggtagtgggt cgattgggtt tcggcattgt
     3661 ttccgttaat gccaattgca tttttgtatt tgatgcaaca gaaggcgtga gattctcctg
     3721 ggcctggaaa ggataagtgt ttagaattga tggcaaaata tcttccatcc gatttttgaa
     3781 gtagttgttg tttggctgtc tcccaaaggg tgttgttaca aacttcttga atttgtagtt
     3841 tttgcaggac tcttattact gcgttatcat gttccaattc tttaggggag tgtattattc
     3901 tctcttttaa taagctgttt accacagggt ttagttgttg tctaaagcac gcagataatg
     3961 ccattgctac acaattttgt tcccgaggaa gcatgtttgg cggggtatgc cgtgtttgat
     4021 gtgatatgtc tattctcata gaaggtctct ggcttatagc atcaacaaat acggctataa
     4081 aacaaaattt tcatagagtg attcaagatc ttcttgagat ccttttttgc ggcgcgtaat
     4141 ctgctgcctg caaac
//
LOCUS       DAEBXD010000219         4083 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEBXD010000219 DAEBXD010000000
VERSION     DAEBXD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.221"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..239
                     /locus_tag="KJL94_004101"
     CDS             <1..239
                     /locus_tag="KJL94_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD4412214.1"
                     /translation="LSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQA
                     QVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(440..1297)
                     /gene="sitD"
                     /locus_tag="KJL94_004102"
     CDS             complement(440..1297)
                     /gene="sitD"
                     /locus_tag="KJL94_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBD4412215.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="KJL94_004103"
     CDS             complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="KJL94_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBD4412216.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="KJL94_004104"
     CDS             complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="KJL94_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBD4412217.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="KJL94_004105"
     CDS             complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="KJL94_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBD4412218.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1100 a         1018 c         1000 g          965 t
ORIGIN      
        1 gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat atggcgtgga
       61 gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa ctggcggcag
      121 caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag cgggagctga
      181 aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc gtggaataac
      241 agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa ctttcgatag
      301 tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc atgatagaat
      361 tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct ggcggacgat
      421 gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gcgtttccct
      481 ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc gcgaataaga
      541 cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca ccagtcattg
      601 acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga
      661 gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata cctactgctt
      721 tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc agccatctcg
      781 tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
      841 attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg attatatcgc
      901 cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttaa
      961 ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccataactg
     1021 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg cagaatactc
     1081 cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc acgatgccag
     1141 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga
     1201 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
     1261 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgctttatg
     1321 cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg ctaaaagaaa
     1381 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
     1441 ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga gcaggcgggg
     1501 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
     1561 aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
     1621 gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt cgaaaaaggt
     1681 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
     1741 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
     1801 gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
     1861 gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct
     1921 gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
     1981 tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca gcgaccagcc
     2041 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg ccgagaccca
     2101 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tcttgcttcc
     2161 ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt aataatgctt
     2221 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctccaggttc
     2281 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
     2341 gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag catcgttttg
     2401 ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt cacatcaacg
     2461 ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc tctcgccaga
     2521 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg aaaatccacc
     2581 atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt ggctatacgc
     2641 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
     2701 gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg tagcgcctgt
     2761 cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc
     2821 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca
     2881 cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
     2941 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc ccggctttaa
     3001 ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta ggtaccgggc
     3061 cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac
     3121 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
     3181 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
     3241 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
     3301 ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca
     3361 ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt tggtaggttt
     3421 gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag
     3481 cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt ccttcggtga
     3541 tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt
     3601 aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct
     3661 gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg
     3721 ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc gcgatgatgg
     3781 taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg
     3841 tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata atttagtacc
     3901 tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca taacctatac
     3961 aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta ggttgtttcc
     4021 tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg aaatgacttt
     4081 atg
//
LOCUS       DAEBXD010000220         3975 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEBXD010000220 DAEBXD010000000
VERSION     DAEBXD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.222"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..224
                     /locus_tag="KJL94_004106"
     CDS             <1..224
                     /locus_tag="KJL94_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD4412219.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="KJL94_004107"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="KJL94_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD4412220.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="KJL94_004108"
     CDS             518..1108
                     /locus_tag="KJL94_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBD4412221.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="KJL94_004109"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="KJL94_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBD4412222.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="KJL94_004110"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="KJL94_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBD4412223.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="KJL94_004111"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="KJL94_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBD4412224.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3456..3647
                     /locus_tag="KJL94_004112"
     CDS             3456..3647
                     /locus_tag="KJL94_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412225.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1074 a          988 c          988 g          925 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc tggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa aaaaacctgt tcactgcctg tcctgtggac agacagatat
     3121 gcacctccca ccgcaagcgg cgggccccaa ccgaagccga tttagttaca acatgcaaac
     3181 ataaacccct aaaaaaccca agtccaacac agaactgaaa ccacaacgcc cccctcataa
     3241 ctgaaaagcg ccgccgcccc cgcccaaagg gcgggaacag agtcgctatt aattatgaat
     3301 gttgtaacta catcatcatc gctgtcagtc ttctggctgg acgtatcgag tacacgctcg
     3361 taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg
     3421 tcgggaccac tccgaccgcg cacagaagct ctatcatggc tgaaaacggg tatggcttgg
     3481 cagggctggg gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg
     3541 gcggttttac tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg
     3601 gcatcagaat aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg
     3661 gtcgtttcag ggatatcgtt aagacttgag cgataggaat taaaacccca aaaagattaa
     3721 aaaaacacca caaaacggat gtttcttcaa caccactttt gctccatatg aacggaaccg
     3781 acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt ttgctccgtt
     3841 tccctcaaaa tggacgcaac ttcccctctg cggctctcag ccgcaccacc gcatccgggc
     3901 cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca gccccgtaaa
     3961 acgcatctga ctccc
//
LOCUS       DAEBXD010000221         3804 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEBXD010000221 DAEBXD010000000
VERSION     DAEBXD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.223"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..1817)
                     /gene="traG"
                     /locus_tag="KJL94_004113"
     CDS             complement(<1..1817)
                     /gene="traG"
                     /locus_tag="KJL94_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBD4412226.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISN"
     gene            complement(1814..3187)
                     /locus_tag="KJL94_004114"
     CDS             complement(1814..3187)
                     /locus_tag="KJL94_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBD4412227.1"
                     /translation="MLRIKPLFILCAALLTAIPTASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(3174..3599)
                     /gene="trbF"
                     /locus_tag="KJL94_004115"
     CDS             complement(3174..3599)
                     /gene="trbF"
                     /locus_tag="KJL94_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBD4412228.1"
                     /translation="MNHRKYQRKLIMKEKRNDAELKNRKTKRDYDYERRVSDIYFDLF
                     FVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLI
                     VYVRNPLNELINGGIRIFRLKRRMRRENKLREGGNNASH"
     gene            complement(3547..>3804)
                     /gene="trbJ"
                     /locus_tag="KJL94_004116"
     CDS             complement(3547..>3804)
                     /gene="trbJ"
                     /locus_tag="KJL94_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBD4412229.1"
                     /translation="AGISGIATGIIVSLNIPFIRQGLFYPASPVEIVVSLCLTFSVSV
                     VFFVGAIVGWISVSEIYYSRMNGLNESSEISEETYNERKKK"
BASE COUNT          935 a         1033 c          913 g          923 t
ORIGIN      
        1 tcattactga tattatgcgc cttcgcatag ctttccactg cactgcgcat acggctggcc
       61 atcatggagt cctgtgcgct catcgtgctg tcagcaccac ctgtgacaga atcactgctc
      121 ccccggttag aaccaaactg actgagcgag ttccaggcac tggcaatact gctgctgaac
      181 ccatgcaggg cgctttctgc tctgttcgac gcctcccggg ccatttcctg ttgtgccgcc
      241 gcaatctgac gcgttgcatt gatgccgacc ggtaaccggg acatcgcccc gctcgcatcc
      301 atcaccatat taccgtcacg ggtctgtgtg gcagttgcgc cactgccggt ctggtacatc
      361 atctgaccaa acgacgtggt gctgttggtg ctccagctga agccgttcac gttttccgtc
      421 tgcatgttgc cgtaggagta attaccgtca accacactac ctgccgcact ggcagtcggg
      481 ctgatagcag aggaagcgaa atggctgtac acgctggaaa aacctgcgcc aaggcctttt
      541 accatcatcc atgacaacgg gggtatcatg acagatatat atccggcagt ggacgccagg
      601 tcggagtatt tcagctgtat ctgagagagt tcagagagca cgaccggtgc accattctgc
      661 tttgcataga atgtcatggc actgttcaga atggcataca gcaacggcca ggtctggaac
      721 cacatcagag caaacacata gcctctcaga acagacagcg tcagtctgtt gaagacagcc
      781 gccagtacca ggagcgggaa gataccaatg gtaatgccca ccagaatggt ctgcaccatc
      841 ggcaggtttc gcatcagcac atgaccaatg gagacatgtg ccagacgctg tttctccatc
      901 gacgaagtcg tggccagact gaccaggctg gcggtatcac cgttacgggc tgcattactg
      961 gtgataccct ctttcagggc attcatggtg acgttctggc gcataatatg actggcagac
     1021 tggctggagc cgtagaaata actgtaactg tcactcacca gttgcctgaa taacaggtcc
     1081 gggtccggtc tgccgccaaa tatctgctgc acataataat gccaggtctt gcctcccgtc
     1141 tttgtatcga gattcagcct gtctttcagc gtgaccgacg catctttaca ggttatgaaa
     1201 ttattattgc tgtcataaac gccccgtagc gggctgggcc gggaaaaaat cagcgtgtag
     1261 gggtcaccgg aggccatcag gtcttccagc gtgtatttgt ggttcaggta aatatcaccc
     1321 agcacgcagt tctggacgta gtcctgaaac agattgatga tttccggatt ccgggacaga
     1381 aaatcggtgc ttttcacaat caggttcgcc ccgaacagca tgcctgtctt gctgtaggta
     1441 gcgctgtcag gctgggtgaa tatcatctcg tagctggcca ccattgcatg cccgatacgg
     1501 gtcgtcagtg aaagtggcat cgccagtccg accggcacat tatccacccg gtgaacctgg
     1561 accaggtcac tgttatcaat aatctgcacc gatgtacgga cattaaccag caggctgata
     1621 agcacaaaca cggccaccca gcccagcaaa tccatcacgt tgtgccgctg tacccacatc
     1681 acggccaccg cgagaacaga caatgtgagc gcaatttttt cgatggaatc ccacgtccgg
     1741 gttcccataa aggcggcaat ggcgttcagg ttattgcgta accactcacc accggcaatc
     1801 acatagactt cattcacagc gtgctccctc cgaagtgata gttgttctgg taacggctga
     1861 gcatgcgggc ggacagctgc tggcgcatat agctcatctg acggtcaaca accagcaatg
     1921 catcctgctg gacctttacc tgcccctgga aaaccgcaat ctgttgctgt gcctgattca
     1981 gattctccag cacactgtcc agcacggtct gtggatagtt cccggtggca atcatggccc
     2041 gggcctgctg aagcagttcc tgaatgtact ggagcatgat gtcgtagccg atatagtccg
     2101 tcagctggta aaccacgctg ctggacacac cgagcatctg cgggtcaata agatatttaa
     2161 agacggggac ggtggtgctg gaaatgaagc ctttttccct gtcatccagt ggagtatcac
     2221 tcaccgcttt attctgaata ctggtgagca gttttgtaat ctgtgatttc agggctttat
     2281 cccgggcgat ggtcacattt gcatccgcca ccaccttcag acacttgtcc gaatcgttgc
     2341 agtgataaac ttttgccgtt cccccctcca taatcgcccg gaggatatcg cggtcagtgg
     2401 tacgcgccgg caggggggta atctcgctgt cttccccaaa aatcagcgta ccggtgagcg
     2461 tcataataaa ttctttcagt tctttgttac catcaagcat cctgtttttg gacagcgagt
     2521 cccagatgat attgatgttc ttcagcaccc gttccttatc tttgtcactg gccttgtcac
     2581 gaacagacga tgactgtcct ccgaccgtgc agccctgacg ggacgcggcc cagtcagcaa
     2641 aaatattgct ctcaccggca atgtcctgac agaccttctg ctgtgacacc tgagtctgag
     2701 gaaataatcc tccgacaatc ccctgcgcca cctgacagga gctgaggttc atactgttaa
     2761 tgtcgcttgc cattttctgc aggaagtctt ttgcggtttt gatttccggc accgttgcct
     2821 gcagggcaag gtcaaaaaag taaccggcag cattgctcat aatctgctta acaaaacgct
     2881 gcaactgatc gccattaata aaactgaacg agccgagata ggcgtcgatg ccgccacatc
     2941 cggcattgat atccggcagc gtcatggaaa tcagctggat atttttgacc tgggtgcggg
     3001 catacaggga gccaccatag gcatacccgg cggcctgccc ctgccagacg cccggctgtg
     3061 tggtattgga ggcgaagccc agcttgttaa agaactgatt catgtcgctg ttcacatccg
     3121 ctgacgctgt gggtatggcc gtcagtaagg cggcgcaaag aataaaaaga ggtttaatgc
     3181 gaagcattat tgccaccctc ccttaatttg ttctcccggc gcatacggcg tttcagccgg
     3241 aatattctga tccccccgtt tattaattca tttaaagggt tcctgacgta tacgataaga
     3301 aatccacctg caaacgccca taacgtatac ggaatgacat acatcaggat gttccacata
     3361 taacgaaaat tatttaattc cgggtcagct ttccacgaat caatacaggc atcaaaaata
     3421 ctatgcataa tcacccataa aaaagttccg gcagcaacga acacaaaaaa cagatcaaaa
     3481 tatatatcag aaaccctgcg ttcataatca taatcccgtt tagtttttct gttttttagt
     3541 tccgcatcat ttcttttttc tttcattata agtttcctct gatatttccg atgattcatt
     3601 cagaccgttc atgcggctgt aatatatttc agacaccgaa atccatccca caattgcccc
     3661 cacaaaaaac acaacagaaa cagaaaaggt aagacacagc gaaacgacaa tttccacagg
     3721 gctggcggga taaaaaagcc cctgacggat aaaggggata ttcaggctga cgattattcc
     3781 cgtcgcgatg ccggatattc cggc
//
LOCUS       DAEBXD010000222         3786 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEBXD010000222 DAEBXD010000000
VERSION     DAEBXD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.224"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            101..694
                     /gene="tetC"
                     /locus_tag="KJL94_004117"
     CDS             101..694
                     /gene="tetC"
                     /locus_tag="KJL94_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBD4412230.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(807..2012)
                     /gene="tet(B)"
                     /locus_tag="KJL94_004118"
     CDS             complement(807..2012)
                     /gene="tet(B)"
                     /locus_tag="KJL94_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBD4412231.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2094..2717
                     /gene="tetR(B)"
                     /locus_tag="KJL94_004119"
     CDS             2094..2717
                     /gene="tetR(B)"
                     /locus_tag="KJL94_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBD4412232.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2695..3381)
                     /locus_tag="KJL94_004120"
     CDS             complement(2695..3381)
                     /locus_tag="KJL94_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD4412233.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3389..3775)
                     /locus_tag="KJL94_004121"
     CDS             complement(3389..3775)
                     /locus_tag="KJL94_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBD4412234.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
BASE COUNT         1250 a          809 c          688 g         1039 t
ORIGIN      
        1 gttcaatata cataaaataa cctaaatgtt cttaagattg tcacgaccac atcatcatga
       61 taccataaac atactgacgg tatgttattt taaatctatc atggaaaata aaaatcatca
      121 acaagaaaat tttaagagta cctatcaatc actggttaac tcagcacgaa tattgtttgt
      181 tgaaaaaggc tatcaagctg tttcaataga tgagatctcg ggaaaagcgt tggtgaccaa
      241 aggtgccttt tatcatcact ttaaaaataa aaaacaatta ctcagtgcct gttataagca
      301 gcaattaatt atgattgatg cctacatcac aacaaaaact gatttaacaa atggttggtc
      361 tgccttagaa agtatatttg aacattatct tgattatatt attgataata ataaaaacct
      421 tatccctatc caagaagtga tgcctatcat tggttggaat gaacttgaaa aaattagcct
      481 tgaatacatt actggtaagg taaacgccat tgtcagcaaa ttgatccaag agaaccaact
      541 taaagcttat gatgatgatg tgcttaaaaa cttactcaat ggctggttta tgcatatcgc
      601 aatacatgcg aaaaacctaa aagagcttgc cgataaaaaa ggccaattta ttgctattta
      661 ccgcggcttt ttattgagct tgaaagataa ataaaataga taggttttat ttgaagctaa
      721 atcttcttta tcgtaaaaaa tgccctcttg ggttatcaag agggtcatta tatttcgcgg
      781 aataacatca tttggtgacg aaataactaa gcacttgtct cctgtttact cccctgagct
      841 tgaggggtta acatgaaggt catcgatagc aggataataa tacagtaaaa cgctaaacca
      901 ataatccaaa tccagccatc ccaaattggt agtgaatgat tataaataac agcaaacagt
      961 aatgggccaa taacaccggt tgcattggta aggctcacca ataatccctg taaagcacct
     1021 tgctgatgac tctttgtttg gatagacatc actccctgta atgcaggtaa agcgatccca
     1081 ccaccagcca ataaaattaa aacagggaaa actaaccaac cttcagatat aaacgctaaa
     1141 aaggcaaatg cactactatc tgcaataaat ccgagcagta ctgccgtttt ttcgccccat
     1201 ttagtggcta ttcttcctgc cacaaaggct tggaatactg agtgtaaaag accaagaccc
     1261 gctaatgaaa agccaaccat catgctattc catccaaaac gattttcggt aaatagcacc
     1321 cacaccgttg cgggaatttg gcctatcaat tgcgctgaaa aataaataat caacaaaatg
     1381 ggcatcgttt taaataaagt gatgtatacc gaattcgatt gcgtctcaac ccctacttcg
     1441 gtatctgtat tatcacgtgt atttttggtt tcacggaacc aaaacataac cacaaggaaa
     1501 gcgacaatat ttagcaacgc agcgataaaa aagggactat gcggtgaaat ctctcctgca
     1561 aaaccaccaa taataggccc cgctattaaa ccaagcccaa aacttgcccc taaccaaccg
     1621 aaccacttca cgcgttgaga agctgaggtg gtatcggcaa tgaccgatgc cgcgacagcc
     1681 ccagtagctc ctgtgatccc tgaaagcaaa cggcctaaat acagcatcca aagcgcactt
     1741 gaaaaagcca gcaataagta atccagcgat gcgcctatta atgacaacaa cagcactggg
     1801 cgccgaccaa atcggtcaga catttttcca agccaaggag caaagataac ctgcattaac
     1861 gcataaagtg caagcaatac gccaaagtgg ttagcgatat cttccgaagc aataaattca
     1921 cgtaataacg ttggcaagac tggcatgata aggccaatcc ccatggcatc gagtaacgta
     1981 attaccaatg cgatctttgt cgaactattc atttcacttt tctctatcac tgatagggag
     2041 tggtaaaata actctatcaa tgatagagtg tcaacaaaaa ttaggaatta atgatgtcta
     2101 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
     2161 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
     2221 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
     2281 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     2341 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     2401 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     2461 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     2521 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     2581 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     2641 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     2701 gtgaaagtgg gtcttaaaag cagcataacc tttttccgtg atggtaactt cacggtaacc
     2761 aagatgtcga gttaaccacc ctttagattc ataaagcgaa aataatgcgg ctccaacgta
     2821 cccacctaaa tggaaacggc gttcactcca atctaaacac gcacaacaga ttttacgtga
     2881 atgtttggaa ggaacgtcaa ttcccatttc atgaaaatat tgaataccac ttaatgtgat
     2941 cattgaacca ttttcagtga tccattgctg ttgacaaagg gaatcataga tcttaacggc
     3001 aacttcgcca gctaaatgat catagcaagt acgtgctttt cgtaaatgca ctggcgtgga
     3061 aactttggca tgtacgccat ggtttaagga gatccccatc atactttcca tcaattcagc
     3121 aatatctttt cctgctagcc gaaaataacg atgcttgcct tgagctacta ctgtgattag
     3181 ctggcaatct aataatttag ataaatgact gctcgccgtt gaagctgata tattcgccac
     3241 agaacttagc tcagtggccg tccaagctcg cccatccatc aaagcactga gtattttaac
     3301 tcgtgaaatg tcagacatag ccgcccctat cgcggctatt gaggactcaa aggtaacctc
     3361 ttttcgtatt aaattagcca tcgcaagttc actttattgc ccaagggagc gtaacagatg
     3421 cagccatact atcattgtcc gttattaata tcagttggtt agcatggtca ctgtattgca
     3481 ctaaaatatt aatgttattc tccgccaata ctcgtgctat ttcgccaagt tcccccggtt
     3541 tttcctgttt taacttacga attaatggtg tccggatcgc aagtactaac agtccagctt
     3601 gctctagcgc tattttagct tcctttcctt gttcaacaag aaaatgagca tggcattcat
     3661 caccaaccgt aaatatccct cccccttcca atccaatacc tttattacct aatgtttttc
     3721 ctagtaatgc taattgtcct atttgattat ctaaaacaac gtgaacatca aacattatcc
     3781 ttgtcc
//
LOCUS       DAEBXD010000223         3752 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEBXD010000223 DAEBXD010000000
VERSION     DAEBXD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.226"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..1515
                     /locus_tag="KJL94_004122"
     CDS             <1..1515
                     /locus_tag="KJL94_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBD4412235.1"
                     /translation="ELGKVQVMVPVLNTANIRDGELRRLSTWENNPDALALVDNVYHR
                     IAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRER
                     GYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETF
                     AIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
                     EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVA
                     LPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLAD
                     NMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVT
                     AEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQERDRAAIT
                     EREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
     gene            1535..2281
                     /gene="traX"
                     /locus_tag="KJL94_004123"
     CDS             1535..2281
                     /gene="traX"
                     /locus_tag="KJL94_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBD4412236.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            2336..2896
                     /locus_tag="KJL94_004124"
     CDS             2336..2896
                     /locus_tag="KJL94_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBD4412237.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            3027..3239
                     /locus_tag="KJL94_004125"
     CDS             3027..3239
                     /locus_tag="KJL94_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBD4412238.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            3483..3722
                     /locus_tag="KJL94_004126"
                     /pseudo
     CDS             3483..3722
                     /locus_tag="KJL94_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
BASE COUNT          884 a          902 c         1215 g          751 t
ORIGIN      
        1 gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat acgcgacggg
       61 gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct ggtggataat
      121 gtgtatcacc ggattgccgg tatcagtaag gatgacgggc tgataaccct gcaggatgcg
      181 gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt cacgctgtac
      241 accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa gagtgaccgg
      301 gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg tgacagtgtc
      361 acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg ggcagagcaa
      421 catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag tgaaaccttt
      481 gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt tgagtcagcc
      541 tacgtggccc tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa ccgtcagggc
      601 tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt gtttgagccg
      661 aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg ggagctgcgg
      721 gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg agacagtcct
      781 gcacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact gccggcgttt
      841 gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga tgacggaaac
      901 gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc gcagttcgtg
      961 gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca ggatggcgtc
     1021 cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg tgaaggccgt
     1081 ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc ggacaacagc
     1141 gtccagccgg gagccggaaa tggtgaaccg gtcacggcag aggtactggc acagcggcag
     1201 gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg
     1261 gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag ggaaattgcc
     1321 gggcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc ggagagtgta
     1381 ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga aaatttgctg
     1441 caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc
     1501 ccgggcggag actgatacag gaagacaaac actgatgaca acggataaca cgaacacgac
     1561 acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc tggtctggtc
     1621 acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac tggaccacat
     1681 caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag gagcctttcc
     1741 gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc
     1801 cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc tggcaggctt
     1861 tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg
     1921 gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg ccctgtgggg
     1981 gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag tcagcaaccg
     2041 gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc tgctggctgt
     2101 tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc ttgtgatgac
     2161 ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt tctggcccgg
     2221 agactttttc ccgacgttct atgcatgtca tctggctgtg ctgggcgttc tggcgctgtg
     2281 acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac acagcatgac
     2341 agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag cgcctgtccg
     2401 cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa
     2461 gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc aggccagaca
     2521 ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata ccctgaagcc
     2581 ctggtggccg ggattatttg acggtgacac gccccggctt ctggcctgcg gtatccggga
     2641 cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa aactgcgcag
     2701 ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg
     2761 ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg aagcgtatgc
     2821 gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac ttcaggccgt
     2881 gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa taaggcttaa
     2941 aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat
     3001 actgacaact aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc
     3061 aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct
     3121 ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg acagactggc
     3181 agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac
     3241 gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt atgagatttg
     3301 cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagtcttttc
     3361 cgcattaagt cgatttttta tattttgtga ttagtttcac aaaataaggt gttattcaat
     3421 gtgtgctgca ctattcaggg gggctgaaat acaggtggca tttatctgat atggagaata
     3481 acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta ttgtctgcag
     3541 atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc atggactcac
     3601 tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg
     3661 gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcacat
     3721 attgaggtgt actggcaata gcggacacta cc
//
LOCUS       DAEBXD010000224         3661 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEBXD010000224 DAEBXD010000000
VERSION     DAEBXD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.228"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            221..1449
                     /locus_tag="KJL94_004127"
                     /pseudo
     CDS             221..1449
                     /locus_tag="KJL94_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1824..2012
                     /locus_tag="KJL94_004128"
     CDS             1824..2012
                     /locus_tag="KJL94_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4412239.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2062..2391)
                     /locus_tag="KJL94_004129"
     CDS             complement(2062..2391)
                     /locus_tag="KJL94_004129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412240.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(2516..2695)
                     /locus_tag="KJL94_004130"
     CDS             complement(2516..2695)
                     /locus_tag="KJL94_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412241.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            2825..3025
                     /locus_tag="KJL94_004131"
     CDS             2825..3025
                     /locus_tag="KJL94_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBD4412242.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            3015..3236
                     /locus_tag="KJL94_004132"
     CDS             3015..3236
                     /locus_tag="KJL94_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412243.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            3229..3449
                     /locus_tag="KJL94_004133"
                     /pseudo
     CDS             3229..3449
                     /locus_tag="KJL94_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3451..>3661
                     /locus_tag="KJL94_004134"
     CDS             3451..>3661
                     /locus_tag="KJL94_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412244.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRY"
BASE COUNT         1022 a          828 c          889 g          922 t
ORIGIN      
        1 accgattata acggatgctt aacgtaatgc gtgaagtatg gacatatttt atcaattagt
       61 gttttttgcc gtttcataat gtttcagatt gttcattatg tttttgatta taaacagatt
      121 tgtgattttc cttgttccag cttgtttcac gttgtagccc tttgtgcaac gatcggtgta
      181 gttaatggtg tagttaattg tgatgaccgg aatcaaaatt atgagcagag cacttaacaa
      241 actgagcgat acacagctga ggaaaatcaa cggcacaccc gcccaaaaaa cagcctttct
      301 taatgacggt ggaaacctga gcgtcaggca ttcaaccagt ggccttttaa cctggtattt
      361 cacttacagg gccggaacgg gaaggggggc accaccggaa cgcattaagc tgggaaatta
      421 tcctgatctg aacctgaaat cagccaggga aaaagccgcc cagtgtcgcg catggctggc
      481 agaggggaaa atccacgtca tgagcttaat tacaccgtac aggaagcgtt aaagccggta
      541 acggttggcg atgcgctcac ctactggctt gagtcgtacg caaaggaaaa ccgcgtggat
      601 tatgccgccc tgaaaaagcg ccttaataat cacgtaatac agcacattgg tgctatgccg
      661 ctggataaat gcgagctacg gcactggctg gcctgttttg accaggtggc aaagcgaacg
      721 cctgttactg ccggattctt gctacagacg tgcaaacagg cgcttaagtt ctgccggagg
      781 cggcgctatg caatcagcaa cgttcttgat gatatgagtg tggcggacgt tgggaaaaaa
      841 ccggatataa gcgagcgtgt cttaagcacc aaagaactgg gcgaattatt gcaggcactg
      901 gacaaaaaaa tattctcccc ctactacatc gcgttaatcc gcctcctgat tgtgttcgga
      961 tgccggacgg tagaactgag gttatcggag atcagcgagt gggattttac cgaaatgctc
     1021 tggaccgtgc cgaaagaaca cagcaaaacg aaggtggcaa tattccggcc cataccggaa
     1081 gcaatactgc cgttcgtcac gcagctggtg gagcagaaca ggcacacggg cttattgctg
     1141 ggggaagtga aacaggaaac aagcgtgtcg cagtacggca gattagcgca caggaggcta
     1201 aatcaccctc actggtcact acatgacatc cggcgcacct ttacaacgat gctgaacgat
     1261 ttaggcgtgg atcctcacgt cgtggagcag cttacaggcc accagatgcc aggaatgcag
     1321 cgagtttata atcattcccg ttatctggat gctaaacgca atgcgctgga tatgtggacg
     1381 gagcggttag ggatactggc gggaacacat gaaaacgtaa ccacgctacc agtagccaga
     1441 agaaaataat ttttttcgtg ttttttcagt atgcgcatac tggatatgcg aacagataca
     1501 acgtgcaaca atgagcaaca aagcggaaca actacgaaca agaggtgaaa aagtgtacag
     1561 atttataaat agctgatttt taatgtgttg ctgatttttt atacactagc gaatcactct
     1621 ttaaatagcg agaaaaaagg catgaaacaa tattttatgc ctttgtatta acatgaattt
     1681 aaaggatttt atcctgatta ttcctctttc tgtaaaccat gcggcctcaa catttgccaa
     1741 ctctgaatca tcctgacaca atcagacaca tcccaacgca atagaacacg aaataactct
     1801 ttaagaaacg aaagggggca atagtgttaa gcactgatcg gtttatacgt gaaaaagaat
     1861 gcgaaaagct aacaggcctt agccgctcat gccgctaccg cctggaaaag gccggacaat
     1921 tcccatcacg tcgtaaactt ggcggtcgtt ccgttggctg gtctttatcc gaggttctgg
     1981 cctggaagga tagctgcaag gcagttcatt aatcacgctg gcggcacgca gccgccacac
     2041 atcaatcatc tgaacacaga gctataacca tgaagattga atatacgcca gaacgtgggc
     2101 ggggattcgt tcgccctggt gaaactggaa aaccacaaaa ttggggtttt tcaggcataa
     2161 aaaaagcggc cccgaaatgg agccgccttt ctgaacagat aacccgctgc gccgtttgtg
     2221 tgtgtgatcc caaacataag cacggggatg atagccgcta tcaggctggt gggcaatgca
     2281 atcagtctgg ttcagtgcgt tgccataact gcgaagttct ctcctttgat gagtattctt
     2341 taaggtactt ctcaagggca aaagaacacg gcgcgaatcg ttctgattca tgctctttct
     2401 ttctgcgtcg tctttttcgt gcaggtgatg ttttttcggt tgattcttcg ttggtcattg
     2461 tgtgcgcctg taaagcaatg cgccgtagta cctcacacca cggcgctgat agtttttatc
     2521 ctttgggttc tatgccgcgt tgctgtaatt ctttgcggat tattcgcttt atccaggcag
     2581 caagagaatc atcgccgtcc tgttgttgtg ccttttccat cttttcccgt aagtcaggat
     2641 ctaaacggaa ctggaacgga ggattaccac gcctttcgtt tttgtgtgtt gacacgttaa
     2701 ttacacctca ggtaatgtat ttatgtgtag tgacacatta cacacaatca acacaaaaag
     2761 caaagccccg cactgtagga gcagtaacgg ggcttctaac caacaacgta aactaggagc
     2821 cgttatggtt gccgtaaatc atataccaca ccttgtacac acacaaacgg cctttgtgtg
     2881 gcgttttctg gcactgagtg ccggagaatc ccaaatcatc cacgtaaccg cttggacgga
     2941 acgcgaagcg cgtagccgtt gcccgtccgg ttgtgttgct gtattcgccg cccgtattcg
     3001 tcagggggaa acctatgcac aataaaacca caccgaacgc agcagccgcc gcactcacta
     3061 cgctgatgca cgcgcttatt gatattgaat gcacggcaga gcttgcgcag ggagaagaac
     3121 agaaagaccg gacacagttc gccctggaat gtatccgata catcgcaacg cggtcactga
     3181 atgacgctaa aaatattctt gttgccgatt gtgaaaatgg ggggggttat gcgtgatgat
     3241 cgttttaatt ccctgaaaca ggaattttcc ggcgttcctg atgatgcggc tgatgcgctt
     3301 tcgtcaatgc cagaacttat tagagcggct tttttcttac tttccacgag agaatataaa
     3361 tcaacgggct tgatgtactg aatatcgccg ccgattatgc tgatttcgtg acagaggtta
     3421 ttttaagaaa aacgacggac ggggattaat atgcgtgata tttaccacga aacaatagac
     3481 cgcgcatttc ttgcactttc tcacagtgaa aacatgctgg aaatattgcg catatggctt
     3541 gaaacacttg gcgacaatga acgcgacaaa caaaaatcaa gaattgccac ggcattaata
     3601 acgcttcttg agcctgtaat aatggaactg caagaaatag atctattgca cgacagatat
     3661 a
//
LOCUS       DAEBXD010000225         3656 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEBXD010000225 DAEBXD010000000
VERSION     DAEBXD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.229"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..221
                     /locus_tag="KJL94_004135"
     CDS             <1..221
                     /locus_tag="KJL94_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4412245.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="KJL94_004136"
     CDS             complement(393..722)
                     /locus_tag="KJL94_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBD4412246.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="KJL94_004137"
     CDS             complement(727..912)
                     /locus_tag="KJL94_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBD4412247.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="KJL94_004138"
     CDS             complement(909..3548)
                     /locus_tag="KJL94_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBD4412248.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT          895 a          976 c          834 g          951 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttga
//
LOCUS       DAEBXD010000226         3590 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEBXD010000226 DAEBXD010000000
VERSION     DAEBXD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.230"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            32..493
                     /locus_tag="KJL94_004139"
     CDS             32..493
                     /locus_tag="KJL94_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBD4412249.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="KJL94_004140"
     CDS             complement(534..1004)
                     /locus_tag="KJL94_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBD4412250.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KJL94_004141"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KJL94_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBD4412251.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KJL94_004142"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KJL94_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBD4412252.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DAEBXD010000227         3586 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEBXD010000227 DAEBXD010000000
VERSION     DAEBXD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.231"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..380
                     /locus_tag="KJL94_004143"
     CDS             <1..380
                     /locus_tag="KJL94_004143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBD4412253.1"
                     /translation="LPASRVYPGSSRQAYLANRALHELRKNRTLPVDEAWVDDFVREA
                     FGRMKKEYVCKDSEMATEGATDLWEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQ
                     NAENVSEYLSLRHQGLQQSIQSL"
     gene            438..1412
                     /locus_tag="KJL94_004144"
     CDS             438..1412
                     /locus_tag="KJL94_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412254.1"
                     /translation="MTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSGKRKEQQ
                     MLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALELMTQIE
                     STVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKIS
                     FPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGWKEYSVD
                     VSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREKVEQLRN
                     TLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            1409..2314
                     /locus_tag="KJL94_004145"
     CDS             1409..2314
                     /locus_tag="KJL94_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBD4412255.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2311..3381
                     /locus_tag="KJL94_004146"
     CDS             2311..3381
                     /locus_tag="KJL94_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBD4412256.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT          904 a          842 c         1036 g          804 t
ORIGIN      
        1 tgctgcccgc ctcccgcgtt tatccaggct catcacgtca ggcgtacctg gctaaccgtg
       61 cgcttcatga gttacggaaa aacagaaccc ttcctgttga tgaagcctgg gtcgatgatt
      121 ttgtcaggga agccttcggt cgcatgaaaa aagaatacgt ctgtaaagac agtgaaatgg
      181 caaccgaagg ggcgacagac ctgtgggaag gctcacttat cgatcaactg ataacggaag
      241 tcatacagag ctcccattcc agagcggcgg cactggcggt tgattctgcc gccgcaaaac
      301 tgatgcagaa tgcggaaaat gtcagcgaat atctgtcatt acgccatcag gggctacagc
      361 aaagcattca gtcactgtag tcgcatatca ccagcctgct tgcggatatc cgggaaatcg
      421 cggactgtca ggagcaggtg accgctgatg tcagaatggc catggaggag atcgatgcca
      481 gaacccggga attactgacg ggggtctgca cctcactgga agaggagctg aatgactatt
      541 tcagaagcgg taaacgcaaa gaacagcaaa tgctggagga agaaaattca gcgcagcccc
      601 gggagagaaa cgcattcgct ttctttcacg atattttcgg aaccggaaat cagcatgaca
      661 ggatgcggga ctttgatccg gacagccccg aaataaaatt cagtgatcgc agggaagccc
      721 ttgaactgat gacgcaaatc gaatcgaccg tgaccagcct gcaccgtgag gctgaagcac
      781 agttccgtcc tgaactggaa aaaatcgtca ggggtattga aacaggtttt cgtggtacgg
      841 ccctgtacgc cacagaaaat attgccggac gcatcaatgc ccgtctggag gatgaaggtt
      901 tcactgtaaa aatcagtttt ccggcagtca gccagttaca gacccggctc gcagtaaaaa
      961 caaatctgag cgcgcttatg gaggaaagaa cggagacagt aacccgtcgc tgtcgacagg
     1021 atggtgtctg gggtacgctt tgtcgatggg caaacaccag tgactggggc tggaaagagt
     1081 acagtgtgga tgtgagccgc agcgtgatca atatcaacaa ggtcagagag gaagtgatgt
     1141 cactgacccg ggcatatttc ggggagctgc aggcatccat tgagcaggat attaaccagc
     1201 ccgtccgcca ggaaatcgat gcctttttct gtacattcag ggagaaagtt gaacagctgc
     1261 gtaacacgct gattcagagc tctgaagatc ataaacgcga tcagcaggcg caggaacggc
     1321 tgaccgggcg tcttcaggcg ttaaatgaaa gggttcctga gctcattact gacagtaagg
     1381 cgttaaggga agaactggag acaatgctgt gacttcacca tttattcaac aaattgctga
     1441 taaccgggta tgtcaggtac tcacctgtct tcctgaaaaa tttgtggttg atttcgcaaa
     1501 cggtattgat gttgcacagg agcatatccg tacggccgga gagcgcacgt tcttccggcg
     1561 gttaaaagag gggctgagcg gcgaaggtac tgcacggcag aatgccatca atgcttcgct
     1621 tgcacagggc gttgaggcgt ccctgcgctg gctgacggag ctgacaacgt cacttgccac
     1681 cacaaactac gcgattaccc gggtaaacga cagggtcagt tcactggtca gtgatactgc
     1741 caggctggcg cattattccg cagatacgcg ggagcagtta ctcaccctgg ccgatcaggt
     1801 tcaccagaaa ctgaatcatc ttgaagaaaa actccaccgt gttgacctgg ttcagcgggc
     1861 gcagttacat cttgagcaga tattctcctg gtggagcgcc gggcgatatg cgtcattttc
     1921 ccctgccgga cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt
     1981 gatacgtcag ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc
     2041 attaacgcag atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga
     2101 ctggcttggt ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg
     2161 gctgggggac tggtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc
     2221 agaacactta ccggttcgca tgccccgtct ctgctctgca gaacgcctct ctgaaagtat
     2281 ggtcgatgaa atatttcaga agggggctgc atgagtatag aaataaaaac agggctggtg
     2341 ctgtccggcg gtggggcggt gggcgcttat caggcgggag tggttaaggc actggcagag
     2401 tgtggtacac agatcagcat ggtttcaggg gccagcattg gcgcattcaa tggtgccatt
     2461 atcgcggcct ctcccgatct gtcagaagct gccgtacgcc tggaggcgct ctgggatcat
     2521 cttgggaata atcaggtgct gtcggtaaac cgatcggttt acttttcatt gctgaaaaaa
     2581 ttgttccagg caatgaacct ctgccagatc cccggacgtg caggagcact gcttacgaca
     2641 cttcttcgcc atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg
     2701 tcagatgggc ccctgacagc gttgatggat cattatcttg atacagatgc tctggcaggc
     2761 gggctaccgc tgtatgtgtc gctgtacccc acagaagggg gcatgcagga tattattgac
     2821 tgcattcgtg ctgaactggg ttccggaacc acgaaaaacg ccgtttttca gcatatccag
     2881 agtctgcccc gcggacagca gaaagaggct ctgcttgcgt cagccgcact gcccctgttg
     2941 ttccgtcccc gtgaggttca ggggacaatg ttcggtgatg gtggtatggg aggatggcga
     3001 aatatgcagg gaaatacccc tgtaacgcct ctggtcgatg ccggatgcaa tatggtgatt
     3061 gtgtcgcatc tgagtgacgg ttctttatgg gatcgccagg cttttccgga caccacaatc
     3121 cttgagatcc gtccccggaa aaggctgaaa tatgcaggtg atggtggcaa cagtggcggt
     3181 ctgctcagtt tcacgtcggc acataccgac gcctggcgtc agcagggcta cgaggacacg
     3241 atgctgacga tggagcatat ccggaaaccg ctggcagcac gtcaggcact gtcccggtca
     3301 gaaacggtgt tgcagaaaag tctggagata acggaagagg cagatttggc actgagaagc
     3361 gcgatggccc ggattaaata aagacgcccc aaagaaacgc caccgcctgt ggtggtgttt
     3421 ttcctgtctg gtgttgttga aaatattttc agatatatat tacctgggtg agatttaaga
     3481 ttaacgcatt tattaatgtc ataaggagag ttatcatgaa attcatcgta aaagcactta
     3541 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttga
//
LOCUS       DAEBXD010000228         3559 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEBXD010000228 DAEBXD010000000
VERSION     DAEBXD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.232"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            85..291
                     /locus_tag="KJL94_004147"
     CDS             85..291
                     /locus_tag="KJL94_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412257.1"
                     /translation="MKSVTIEAKTFAEMLGITEGELIFAIKKTGTFKNKTIPQPHEPH
                     KSNNRFLYSDVMRFIESLKDKENR"
     gene            complement(302..574)
                     /locus_tag="KJL94_004148"
                     /pseudo
     CDS             complement(302..574)
                     /locus_tag="KJL94_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            703..2106
                     /locus_tag="KJL94_004149"
     CDS             703..2106
                     /locus_tag="KJL94_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBD4412258.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTLAEEELLRKMLPEAPAHHPDYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(2140..3354)
                     /locus_tag="KJL94_004150"
     CDS             complement(2140..3354)
                     /locus_tag="KJL94_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HBD4412259.1"
                     /translation="MLMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLYIPS
                     GIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIYYNSRHFGKTRNEKRITRW
                     GRGSPLQDPENTGALTLLAFKLDEQGGDCKEVNIWVCASTDEEDVIETAIGEVIPGAL
                     ISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEIIQYAASHYVKNSLDPDEQL
                     LDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSVSNRRKSRAGKSLELHLEH
                     LFIEHGLRHFATQAITEGNKKPDFLFPSAGAYHDTEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSLHKKYPEAVRAELMTLGAF
                     IAELTGLYADIP"
     gene            3410..3475
                     /locus_tag="KJL94_004151"
                     /pseudo
     CDS             3410..3475
                     /locus_tag="KJL94_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          984 a          844 c          846 g          885 t
ORIGIN      
        1 tgaagcgtac cgcagcaaca ctagaaacga gcaccattgt taaagatgcc ggtaaagaaa
       61 atcgatacca tgagagttaa ttaaatgaag tcagtaacta tagaagcaaa aacatttgct
      121 gaaatgttag gaataacaga aggtgagtta atctttgcca ttaagaaaac tggcacattc
      181 aaaaacaaga ccatcccaca acctcatgag ccacataaat caaataatag atttttatat
      241 tcagacgtaa tgaggtttat agaatcacta aaagacaaag agaaccggta atgactccaa
      301 cttactgata gtgttttatg ttcagataat gcccgatgac cttgtcatgc agctccaccg
      361 attttgagaa cgacagtgac ttccgtccca gccttgccag atgttgtctc agattcaggt
      421 tatgtcgctc aatgcgctga gtgtaacgct tgctgataac gtgcagcttt cccttcaggc
      481 ggggttcata cagcggccag ccatccgtca tccataccac gacctcaaag gccgacagca
      541 ggcccagaag acgctccagc gtggccaacg tgcgttacct aacaataaac ctgtttaaat
      601 atccagataa aaacattcaa tctgggtcaa atgagtgata cagtttcacc cataagaccc
      661 aatggaggca atatgtctga atttgaatta ctggcgcagg atctgcttga gaaagcagaa
      721 gcggaagaac aactgcgaca ggaaaatgat aaaaagctgc tcgggcaggt gctggaaatc
      781 tatgaccaga agtacgtggc tgaactgctt agaaaagttg gtaaaaatga gtggagtcgc
      841 gagactctta atcgctggat taatggtaag tgctcaccta agacgctgac gttagccgaa
      901 gaggaacttc tacgaaaaat gcttccggaa gcgcctgcac atcaccctga ctatgccttc
      961 cggtttattg acctgtttgc tgggattgga ggtatacgga agggcttcga aaccatcggt
     1021 ggccagtgcg tttttaccag tgaatggaat aaagaggctg tgcgcacata taaagctaac
     1081 tggtttaacg atgctcagga acacactttc aatctcgata ttcgtgaagt cacgctcagt
     1141 gataaacctg aagtacctga aaacgatgcc tatgcttaca ttaatgagca tgtgccggat
     1201 catgatgtac ttctagcagg tttcccctgt caaccgttta gccttgcggg cgtaagcaag
     1261 aaaaactcgc tcgggcgcgc gcatggtttc gaatgtgagg ctcagggaac gcttttcttc
     1321 gatgtggcgc gtattatccg cgcaaaaaaa cctgccatct ttgttcttga aaacgttaaa
     1381 aacctgaaga gccatgacaa gggtaaaacc tttaaagtca tcatggatac cctcgacgaa
     1441 ctgggctatg aagttgcgga tgcagctgag atgggcaaaa acgatcctaa agttatcgac
     1501 ggaaagcact ttttacctca gcaccgagaa cgtatcgttt tggtcggttt ccgccgtgat
     1561 ctgaacattc accagggctt taccctgcgc gatattagtc gtttttatcc ggaacagcgt
     1621 ccgtcatttg gcgaactgct ggaacccgtg gttgacagca aatatatact gacgccgaaa
     1681 ctctgggagt atctctataa ctacgccaaa aagcacgcag ctaagggtaa cggattcggt
     1741 tttggcctcg ttaatcctga aaataaagaa agcattgccc gtacgctttc tgctcgctat
     1801 cacaaagacg ggtctgaaat tctgatagac cgtggctggg atatggccac aggtgaaaca
     1861 gacttcgcga acgaagaaaa tcaggcgcat cggccccgca ggctgactcc gcgagagtgc
     1921 gcgcgcctta tgggttttga aaaagtagat ggcaggcctt ttcgcattcc tgtgtcagac
     1981 actcagtcgt acaggcagtt cggtaactcc gtagtggtgc ccgtgtttga agccgtagcc
     2041 aaactgcttg aaccttatat cctgaaagcg gttaatgccg attcgtgcaa ggttgaacga
     2101 atctgatcgc tcctcccggt atttatgccg ggagataatc tatggaatat ctgcgtaaag
     2161 ccctgtcagc tcagcaataa acgcacctag cgtcattagc tcagctctca ccgcctccgg
     2221 gtattttttg tgcagagatg atggcacgac caatctgaca cccgactccc gcatctcccg
     2281 atattgagcc agagaaactc cctcctggag tgtaaacaga tgcacctgat gaattttatc
     2341 ggcctcattc agtatctgac gccagcgatc cttacaggta gtcttgactg ccagcatgcg
     2401 cagattttct acgggaaact cagtatcgtg gtaagcccct gcggaaggga aaaggaaatc
     2461 gggtttttta ttaccttctg tgatggcctg cgtcgcaaag tgtcgcaggc cgtgctcaat
     2521 gaatagatgc tccaggtgca gttccagcga cttcccggct ctggatttac ggcgattgct
     2581 gacagaattg gccagcgcaa taaattcatc cacagagcca aatcctttcc ggatgatatc
     2641 cagaacatgc agttcctcaa ccaatagaaa tatgtcgtac tccacgcgcc ggcggtcaag
     2701 aagttgctca tccggatcaa gggaattttt cacataatgg ctggctgcat actgaataat
     2761 ttcacttccc gacggaaagc gcaggtgcca gtcttcaggt agaatatatt tatgatttac
     2821 tggcgcttgc tgtagagata gtccgcctag aatctgtcct gcggggccgg atataagcgc
     2881 tccgggtata acttcaccaa tagcggtctc aatgacgtcc tcttcatcag tgctggcgca
     2941 tacccaaata tttacttcct tacagtcccc cccttgctca tcaagcttga aagccaggag
     3001 cgtcagagcc cctgtatttt caggatcctg aagtgggctg cctctacccc agcgggtaat
     3061 ccttttttca ttccgggttt taccaaaatg acggctgtta taataaattg ccctggcttc
     3121 gctgtcaggg caatcatgcg atgacacatg tgcggtgaga aaaaccgaag ggttcagttc
     3181 acgggtatgg ttgatagacg gaaagagttt ttcaacgata cctgaaggga tataaagccc
     3241 tacctggtga ccacctgttg cgccggtatc gttggcggaa aggcgtttga tgtagacgaa
     3301 gtaattctca catgcgatct caagtagcca gttgtggaaa accgacataa gcatcccctg
     3361 ttaccctgaa actctactca ccattttttc atgattatat acaaacagtg tcattttcag
     3421 aagacgactg caccagttga ttgggcgtaa tggctgttgt gcagccagct cctgacagtt
     3481 caatatcaga agtgatctgc accaatctcg actatgctca atactcgtgt gcaccaaagc
     3541 gaggtgagca tggcgacgg
//
LOCUS       DAEBXD010000229         3548 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEBXD010000229 DAEBXD010000000
VERSION     DAEBXD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.233"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KJL94_004152"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KJL94_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD4412260.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KJL94_004153"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KJL94_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBD4412261.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KJL94_004154"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KJL94_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBD4412262.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAEBXD010000230         3404 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEBXD010000230 DAEBXD010000000
VERSION     DAEBXD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.234"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            67..783
                     /gene="nanC"
                     /locus_tag="KJL94_004155"
     CDS             67..783
                     /gene="nanC"
                     /locus_tag="KJL94_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBD4412263.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            803..1909
                     /gene="nanM"
                     /locus_tag="KJL94_004156"
     CDS             803..1909
                     /gene="nanM"
                     /locus_tag="KJL94_004156"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBD4412264.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1974..2954
                     /locus_tag="KJL94_004157"
     CDS             1974..2954
                     /locus_tag="KJL94_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBD4412265.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          992 a          724 c          800 g          888 t
ORIGIN      
        1 acataaatgc taattgttta tgcgggcttt gtattgcttt ctgtatccta caaatgagtg
       61 aaatttatga aaaaggctaa aatactttct ggcgtattat tactgtgctt ttcgtcccca
      121 ttaatttctc aggctgcgac actggacgta cgtggtggat atcgtagtgg aagccacgcc
      181 tatgagactc gactcaaagt cagtgaggga tggcaaaatg gatggtgggc aagcatggaa
      241 agtaatacct ggaataccat tcatgataat aaaaaggaaa atgccgcact caatgatgtt
      301 caggttgaag ttaattacgc aattaaactt gatgatcaat ggacggtgcg cccgggaatg
      361 ttaacgcatt ttagcagcaa cggcacacgc tacggaccct acgtaaaact gtcctgggac
      421 gcgacaaaag atcttaattt tggcattcgc tatcgttacg actggaaagc ttaccgacaa
      481 caagacttat ccggtgatat gtctcgtgat aacgttcatc gttgggatgg atatgtcact
      541 taccatatta atagtgattt caccttcgca tggcaaacga cgctatacag caaacagaat
      601 gattatcgct atgcaaacca caagaaatgg gcgacggaaa atgcctttgt tctacaatac
      661 catatgacgc ccgatattac gccatacata gaatatgact accttgaccg ccagggtgtt
      721 tacaacggca gagataattt atcggaaaac agttatcgca ttggtgtgtc atttaaactg
      781 tagtagacag gagacagtca caatgaataa aactataatg gcgcttgcta tcatgatggc
      841 ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct gtgccattta aaagtggtac
      901 cggagcaatt gataacgaca ctgtctacat tggtttaggt agcgcaggta cagcatggta
      961 caagctagat acacaggcca aagataaaaa atggactgcg ttagctgcat tccctggtgg
     1021 accgagagag caagcaacct ctgcatttat tgatggcaat ctgtatgtgt ttggcggcat
     1081 tggcaaaaac agcgagggat tgactcaggt atttaatgac gtacacaaat acaaccccaa
     1141 aaccaatagc tgggttaaat tgatgtcgca cgcgccgatg ggcatggcgg gtcatgtgac
     1201 ttttgtacac aacggcaagg cttatgttac tggcggtgtt aaccagaata tcttcaatgg
     1261 ctattttgaa gatctcaacg aggctggaaa agattcaacc gctatagata aaatcaacgc
     1321 ccactatttt gacaaaaaag cagaagatta tttcttcaat aagtttctgt tgtcttttga
     1381 tccctcaaca cagcaatgga gttacgctgg cgaatcgccc tggtacggaa cggctggtgc
     1441 ggcggttgtg aataaaggtg ataaaacctg gcttattaat ggcgaagcca aaccaggatt
     1501 gcgaacggat gccgtatttg aacttgattt caccggtaat aatttaaaat ggaataagct
     1561 tgatcccgtc tcatcaccag atggcgtcgc tggcggtttt gcggggataa gcaatgattc
     1621 tcttatattt gccggagggg ccggattcaa aggttcacga gaaaattacc agaacggtaa
     1681 gaactatgcg catgaaggcc tgaaaaaatc atatagcact gatattcatc tttggcataa
     1741 cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg gcctacggag tatcattgcc
     1801 ctggaataat agtttattaa ttattggcgg tgaaactgca ggcggcaaag cggtgacgga
     1861 ttcagtttta atttctgtga aggataataa agtcacagta caaaactaac gcttcagggc
     1921 cccggtaaag gggccctgct atcaatttgt catttgtatt acgcgaaaat tatatgaacg
     1981 caataatatc acccgattat tactatgttc ttaccgttgc tggtcagtct aatgccatgg
     2041 cgtatggcga aggactgcca ttaccggaca gggaagatgc gcctcattcc agaattaaac
     2101 aattagcgag atttgcgcat acgcatcccg gaggcccgac atgtcacttt aacgacatta
     2161 ttccactgac tcactgccca cacgatgttc aggatatgca gggttatcac catcctctgg
     2221 caacgaatca tcaaacacag tacggcaccg ttggccaggc actgcatatt gcacggaaat
     2281 tactgccctt cattcctgat aatgcaggga ttcttatcgt tccgtgttgc cgtggcggat
     2341 cggcttttac cgcgggcagc gaagggacat attcagaacg gcacggagcc agccatgatg
     2401 cttgtcgttg gggaacggat actccgctgt accaggattt agtcagcaga acgcgagccg
     2461 cactggcaaa aaatccgcag aacaaattcc tcggcgtatg ctggatgcaa ggcgaatttg
     2521 acttaatgac cagtgactac gcgtcacacc ctcaacactt taatcatatg gttgaagcct
     2581 ttcgtaggga tctaaaacaa taccattctc agcttaataa tattactgac gcgccgtggt
     2641 tttgcggcga taccacctgg tactggaaag aaaatttccc gcattcgtat gaagctattt
     2701 atggcaatta tcaaaataat gttttagcca atattatttt cgtcgacttc cagcaacaag
     2761 gtgaaagagg actgacgaac gcgcctgatg aagacccgga cgatttaagc acgggatatt
     2821 acggttcagc gtaccggtca ccggagaact ggacgacggc actgcgaagc agtcatttca
     2881 gcacggcagc ccgtcggggg attatttctg acagatttgt agaagcaatt ttgcagtttt
     2941 ggcgcgaaag gtgagcgggg agtttattca tgggggatgt ccttgttcgt tctcagagta
     3001 gacaacaaaa aacaaatgag atcaaggtaa tatctcttca cttgcgggct cgaacagcag
     3061 gcaattgcca atataataat ctggcacaag ggtgaaaaac gcgcaccgca taaaccgttg
     3121 ttattgctta cagattagcc ggatgcctta atagacatcc gcgccttttc gattatggct
     3181 cggaaatcta cgaacatcta cacagtttac tggaacgttt tgggccacag cgttcgcagc
     3241 accgtcctga tatgccgttc tggcgattat aagtcgacgg attctggcaa atagacgatg
     3301 atgatatgct taatgatgtt caaagaaatt aggccattat tatattattg cttgcatttt
     3361 ctttcattcc taaaagcacc cgccattaat cccaggcgca ttat
//
LOCUS       DAEBXD010000231         3307 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEBXD010000231 DAEBXD010000000
VERSION     DAEBXD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.236"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..149)
                     /locus_tag="KJL94_004158"
     CDS             complement(<1..149)
                     /locus_tag="KJL94_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBD4412266.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
     gene            complement(255..1271)
                     /gene="phnD"
                     /locus_tag="KJL94_004159"
     CDS             complement(255..1271)
                     /gene="phnD"
                     /locus_tag="KJL94_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4412267.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1296..2084)
                     /gene="phnC"
                     /locus_tag="KJL94_004160"
     CDS             complement(1296..2084)
                     /gene="phnC"
                     /locus_tag="KJL94_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBD4412268.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2217..2660)
                     /gene="yjdN"
                     /locus_tag="KJL94_004161"
     CDS             complement(2217..2660)
                     /gene="yjdN"
                     /locus_tag="KJL94_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBD4412269.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2818..3153)
                     /locus_tag="KJL94_004162"
     CDS             complement(2818..3153)
                     /locus_tag="KJL94_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBD4412270.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          699 a          848 c          930 g          830 t
ORIGIN      
        1 aaggttgcca tgttgccgcc gtctttaatc agcgtaagcg gggccatttc cgcgccctgc
       61 cacgagacga ccagcacgtc gagaacaacg gcccagctca gaagcgagaa ccagctacgc
      121 ttgggtgggg cgatggtgat ggtttgcatg tttggctccg gtctgtaggc cggataaggt
      181 gcttgcaccg catccggcat caacgcctac acattgtcgg atgcgacgcc ggggcgtctt
      241 atccgaccta cgatttactg caccgcttta ctcaccgaac tcatcgcgct taacgcgttg
      301 ttcaggcggt ccaggtcatc cagctgcgcc tgaatctcgg tggttttcgc cagcttgtcc
      361 tgctcattca gccctttatt gcctttcacg ctttgtatct ctttaaacag tgcgagctgg
      421 cgaatcggca ccagttgcag gtcgctggaa gcgcggaacg gcgcccagcc caggcgttcc
      481 agcaccgctt tctcttccgg cgtttttccg tagttcataa agaagtcgta gatcttgtct
      541 ttggtagttt cggacagatt cttgcgccag acgatcggat cgcctaggat cagcggcgat
      601 ttccagatca ccttcagctc tttaagcttc tccggcgcgg aggttttcag cttgtcgagg
      661 ttttcggtgt tgttggtggc aacatccact tgcttgttgg cgacggccag cgcgttggtt
      721 tcatgcccgg cgttgacggt gcgcttgaag tcgctggcgg agatattgtt tttagcgaag
      781 acgtagtaac cggggacgag gaagccagag gtggagttag gatcgccatt gccgaaggtg
      841 agatctttcc gcttcgccag cagatcgttc aggttgttga tcgggctgtc tttgttgacg
      901 atcaacacgc tccagtaacc cggcgatcca tccgccgcga ccgtctgggc gaagacctgg
      961 ccattggcgc gatccaccgc ttccatcgcc gacaggttgc cgtaccaggc aatatccact
     1021 ttattgaagc gcatcccctg gataatgccc gcgtagtccg gggcaaagaa ggcgttgacc
     1081 ttcacgccca gtttcttctc catatcctgt aaaaacggcg tccattgcgg tttcaggttt
     1141 tgctgtgatt ccgttgaaat aatgccgaaa ttcagcgcct tttcctgctc ttcggcgtgt
     1201 gccggggtta acagggtgct gaggctgaac atgctggtga aggccagcga ggcaattatc
     1261 ttagcgttca ttcgttttcc tcaatgatgg ggatatcagg cagctttcgc gttctcttcg
     1321 atgcggttaa tgctgcggta gagatggtca aaacgttcgt tatcaaacag ttggctgctg
     1381 ccgtcgtaga agacgtgccc ctgacgcagg gcgacgatgc gttcgcagta gcgcagggcg
     1441 taatccacct gatgcagcgt gacgaccacg gtgatgccgt cgttctggtt gatgtcgcgc
     1501 agggtgtcca tcacgatgcg cgctgattcc gggtccagcg aggcgatggg ttcatcggcc
     1561 agaatcactt tcgcctgctg catcagcgca cgggcaatcg ccacacgttg ctgctggccg
     1621 ccggagaggg tggaaacgcg ctgatgggca aaatgcacca tgccaacgcg ggtcagcgcc
     1681 tgtaacgcgc gctgtttctg ctcgccggtg aaccagctaa aacaggtgcg ccagaacggc
     1741 gtgctgccga gcgcgccaat cagcacgttc tccagtacgc tcaggcggtt caccaggttg
     1801 aattgttgaa agatgtagcc ggtgttggcg cggcttttgc ggatatcgcg ggccaggcgg
     1861 ccttcgcgct ggacagtgcg gcccagtagc tcgatatggc taccggcgga tttatcgccg
     1921 gtaatcaaac cgcttaaatg acgtaaaagg gtggatttgc cggaacccga cggcccaagc
     1981 agagccacca tttcactgtg atgaatgttc agatcaaccg catgcagcgc ttgatgctga
     2041 ttgaaggttt tggcgagctt ctcgacacgg ataatcgttt gcatgatgca gcctccctaa
     2101 aaaagtggct ccatcgtggc ggattattgt gacgatttgg ttaattaaaa gtgactaaca
     2161 gatgaagagt taacgggaat tcgatgacag tgcgggaggg cgggcctccc ggcgggttat
     2221 tgcgttggtt gttgtttgac gacattaatc atccacggta cgccaaattt atcggtgact
     2281 ttgccaaagc catgcgccca gaaagtttcc tgccaggcca tttcgatttt tccgttagcg
     2341 gcaaggttat caaaccagcg ttttccttct tcgacctgtt gcgaatcgag caccagcgta
     2401 aagccggagt agctggcttt tcctgacggt atagcatcgc tcatcatgat gtcgcttccg
     2461 gcaatgcgca cgttggcatg agcgatggcg gtatcgggaa attgcattcc ggaagggcag
     2521 ttctcggcgc tgtcctgcgc tgatttcggc atttcgccga agctgatttt atagagcagt
     2581 tccgcgccca acgtacgttg ataataggca atcgcgtcgg aacagttacc ggcaaaagag
     2641 aggtagggac ttaacggcat gatggtgacc tcagttaaga gaagccagtt aagtgtagtt
     2701 cgaaatttat acagatgaga ggctgcctga taagacgcgc aagcgtcgca tcaggcagtc
     2761 ggcatcgttg ccggatgcgg cgcgagcgcc ttatccggct taccgatcac aatacaatca
     2821 gttctttttc acaaactcag atttcagttt catcggacca aaaccgtcga ttttgcaatc
     2881 gatgttatgg tcgccttcaa ccaggcggat gtttttcact ttggtgccaa ttttcagcat
     2941 cgaagagcta cctttcacct tcagatcttt aatgatggta acgctgtcgc cgtcagccag
     3001 cagattgccg ttagcatctt taacgatcag ctcgtcgctt tcctgtgcag gttctgcgtc
     3061 gttccattcg taggcacatt ccgggcagat gtacatgccg ttatcttcgt aagtgtattc
     3121 ggagttgcat tttgggcagt gtggtaatga catgtggatt tcctcaaaag tcagcagggc
     3181 aaaagcgcca aaaaacggca gattgccgaa aaaggccgca attatacaca aaatccctga
     3241 gtttgtcggg ggtactgctg attttagacg acgatcgttt tcatttgaaa ggaaacgtaa
     3301 aaagaaa
//
LOCUS       DAEBXD010000232         3252 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEBXD010000232 DAEBXD010000000
VERSION     DAEBXD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.237"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(129..1031)
                     /locus_tag="KJL94_004163"
                     /pseudo
     CDS             complement(129..1031)
                     /locus_tag="KJL94_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1091..1369
                     /locus_tag="KJL94_004164"
     CDS             1091..1369
                     /locus_tag="KJL94_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412271.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1370..1897)
                     /gene="gadE"
                     /locus_tag="KJL94_004165"
     CDS             complement(1370..1897)
                     /gene="gadE"
                     /locus_tag="KJL94_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBD4412272.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2696..>3252)
                     /gene="hdeD"
                     /locus_tag="KJL94_004166"
     CDS             complement(2696..>3252)
                     /gene="hdeD"
                     /locus_tag="KJL94_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBD4412273.1"
                     /translation="ATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILS
                     TVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIA
                     AFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVST
                     LVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          920 a          723 c          700 g          909 t
ORIGIN      
        1 tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc ataacttatt
       61 gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat aaattaccta
      121 gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata catgccaggc
      181 agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca
      241 tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt accattttcc
      301 agattgagct gtactggcgt actgccctga acctgtttga tttgcccact ggcgacctct
      361 tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat cgggtccaga
      421 cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc
      481 gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg cagattgatc
      541 gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc attcagctgg
      601 gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg
      661 cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag cgagcctttg
      721 gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga atcgccctgg
      781 ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac ctggggacgt
      841 atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac attgaccgaa
      901 ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt ttccgccgat
      961 ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa cagctttctt
     1021 cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta aattgcgcgc
     1081 gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag ccagtaaggt
     1141 gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct
     1201 gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat acacatacca
     1261 ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg aaatcagcat
     1321 ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac taaaaataag
     1381 atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg tacataatgt
     1441 tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg atattgcttt
     1501 catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa aaacataatt
     1561 gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt
     1621 ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg
     1681 ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa
     1741 ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga
     1801 tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct
     1861 gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg
     1921 tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag
     1981 taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata
     2041 cttcagattt atccatcacg cactccatat attgatccta agtaagattc ctggttgtta
     2101 tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt
     2161 atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt aatccgattt
     2221 aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa
     2281 cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct
     2341 ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt
     2401 acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc
     2461 acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc
     2521 taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat aatcactagc
     2581 aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta
     2641 ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg ggtaattatt
     2701 gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa
     2761 ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca aggaatatcc
     2821 aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac
     2881 gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa
     2941 atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag ccgatcaata
     3001 aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc
     3061 tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc actactgtgc
     3121 ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg ataaacagca
     3181 gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga tcaaacttca
     3241 aaattgttgc ct
//
LOCUS       DAEBXD010000233         3230 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEBXD010000233 DAEBXD010000000
VERSION     DAEBXD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.238"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(438..1022)
                     /locus_tag="KJL94_004167"
     CDS             complement(438..1022)
                     /locus_tag="KJL94_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4412274.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(1022..2260)
                     /locus_tag="KJL94_004168"
     CDS             complement(1022..2260)
                     /locus_tag="KJL94_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4412275.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(2257..3162)
                     /locus_tag="KJL94_004169"
     CDS             complement(2257..3162)
                     /locus_tag="KJL94_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBD4412276.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          661 a         1037 c         1001 g          531 t
ORIGIN      
        1 gccgtgctag tctgctcggt gatggtggag tgaagccaac ccgcaatcgg gttatgaatc
       61 tgcatcgcga ttcgcaatca gctgtctctt gagcatgtcg aactcctgcg atgttatctc
      121 gccgtgctcg tgctgctaca ccaacttgtc caactcatcc gccacaccgc tccccaccac
      181 ctgcgatggc caggttgttt gctgggcgca ttggcggagt atcgtctcga cccgggacac
      241 ccacaccggc acaaccttac gggagtgatt cactgtcaaa gaatcggccc ggtgctctga
      301 cgcaagtatc gggatggtca ccatttgtaa gccgtagacc tgagtggtga tcaagacttc
      361 gataccaccg accgtaccgg tactaatcga cgacggtcgt gttcgtcgcc tgccgcaggg
      421 actctgcaca cctccgttta cgcatgtgcc tggaggagtt ggaaatcgtc gtgttcggga
      481 aacattaaac acaggatggc agcgatctga gccagcacat gatcagctag ctcaccatcc
      541 ggatcgacgg cccactgcat cgtcgcgcca gcgatgaccg agtgcaggag caactcagct
      601 gccgcaggag cacctggggg cagtcgcttg cggatcccct ccaccaccgc gcggttccgc
      661 tggatcgcaa gcgtgcgtag ctccggcacc tggagctcgt accaggagat gagatagttc
      721 accgagaagt cgttgcgagt gttcatgctc cgaacgagca cctgcaaaaa ttcccagagc
      781 ccttgcggcc ctgcgcctat cggtatcgca ttcaggtaat gccgcacctg ctcgacgccg
      841 cgctccatca tcctcaccag cagcgtatcg cggttggtga agcgctggat taacgctgcg
      901 cgggagagcc ccacctcctt tgctactccg ctgagcgtga actctatggg accgcaacgc
      961 ttcagcacta cggtggcggc ctcgagtacc tcgtcatcgg acttgagctt ggggcggggc
     1021 atcagtgttc accttctgta tgggttgggg cggaggctgt ggctgccgcc gccattgtag
     1081 caaattgaag acggagcgag agtagagcca cgagccccgc aaacacggcc gatacaacga
     1141 ggccagggag cggaccagca aggtcgacaa acgcgccggc cgcaagcata accatgggcg
     1201 aggctgacag catcaccgcc gagaccgtgc cgagtacccg gccgagaagt tctggcggcg
     1261 tgcggttgta gatggcagcg ttgagaatgg gagagactga gccggtcagc agtcccacga
     1321 gcgcgcccaa caacatcagc accggcacgc ctggcaactg tgaaagcaga agcgagccca
     1381 ccgcagagcc acaaaatgcc accgccagcc agttctgcgc tgatatccgg gcgccgaccg
     1441 acgcatgaat ggcaatgcca aggagaccac cagcccccat cattgaggag aacagcccga
     1501 gctctgctac ttggcgtcct gcatctacaa acagcgcagg catgatgacg ctgccgttgg
     1561 cgccaacgat gcccacgaag atcatcacta taccaaagag agggcgcagc aggggttcgc
     1621 tccagagaaa agcgacgccg gcgcgcatgg agagagtcgc cgtcgtggtc atcgtccgag
     1681 cggcacgcgc gggaagcacc cacgcgccga gcagacctgc aaggacggag cagaacgccg
     1741 tcagcccgag cgttggcgca gcgccaagca ggccgattgc ggccccccca agggccgggc
     1801 cacctagaat cgcgacgttc ccgatcaccg ctttcagtga cgagacgcgc tcaacggaga
     1861 gcccggcgac gtggccgagt ttgggcagct cactgtcctg cgcggccata ccgggtgcgt
     1921 cgaacgcggc accgagcacc acgcaagcga tcagcccagt gttcgagagg gcgccaacgg
     1981 catcgagcag tgggatgctc gccatggcca cgccgcccac cacacccgag atcaatgcga
     2041 cgggcgcgcg cccgaaccga tcgacgaggc caccaccaac ccacgcgccg atgatggtcg
     2101 cgatgacgct gctagcggcc gtggcgcccg cccaggccgc gctctttgta tgagacagga
     2161 cgaaccatgg aagcgcgagg gccgccaccg cgttgccgat ccggaagaga aaggtcgccg
     2221 cgaacagcgt cgcgagcggg ctatatcgac gttcgctcat tccgctgcgg cgagctgcgc
     2281 cttcgccgca gcgaggtact cttcgttacc cgagtcgagg gcgaagagtg cgtaggtgac
     2341 cgccccgaac gcaaggcgct ccgcgatgtg gtgggcgagc cgcggccaca cccggccacc
     2401 ggccgcttca tacgtgagga ggagcttcgc gagcccctct tcaccaaaga ccataaggtg
     2461 cgcggccatg tcgatggcag ggtcatcaac gcgggcctcg ctccagtcga tcatcccgct
     2521 gacgcgctcc gtgttgtcga tgagcacatg gcccacgtag agatcgccat gcaccaccac
     2581 ggagaaatct ggccacgacg aatcgtcgtc gagccagcgc tgccaccggt ggaggcgctt
     2641 gtcgttcacc acgaactcgc gtcggacgcg gtcaacgtcg tcggccacct tctgacgggc
     2701 ctgcgtcggt gtacggatga gcatccccgc atccacggcg gcggaaatgg ggacggcatg
     2761 cagggcggcg agcgcggtcg cgaagctctc cgcgaagacc tccgagtcct gcggcacgac
     2821 ccagtcgggc gtggacgaac caggctggat gaccatcgca gtcgagtctt cgagcatggg
     2881 ataggcaacg agctcggcgt tggccacgcg ccagtccggc accgcgaacg gcaggcgatt
     2941 cttgagcatt gccagcaccc gcgcctctgg ttcgaccttc gcgcttacct cggctcggcg
     3001 cgggatgcgc agcacccacc gacgtccatc gtcgacggtg gcgatcacga tcctatagtc
     3061 gagcccaagc tcattgacag tcagcgggcc atggagcttg agcccatgtc gggctgcaag
     3121 tgcgtacagt tgggaggtat cggcggtcgt gactacggtc atgattcact cctgagggct
     3181 tgacgggttt agccacctaa atgtaacagt cacgtcggtt atattcaatc
//
LOCUS       DAEBXD010000234         3193 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEBXD010000234 DAEBXD010000000
VERSION     DAEBXD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.239"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            102..3005
                     /locus_tag="KJL94_004170"
     rRNA            102..3005
                     /locus_tag="KJL94_004170"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3193
                     /gene="rrf"
                     /locus_tag="KJL94_004171"
     rRNA            3101..>3193
                     /gene="rrf"
                     /locus_tag="KJL94_004171"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          849 a          700 c          988 g          656 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc cat
//
LOCUS       DAEBXD010000235         3180 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEBXD010000235 DAEBXD010000000
VERSION     DAEBXD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.240"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="KJL94_004172"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="KJL94_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBD4412277.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="KJL94_004173"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="KJL94_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          658 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaac atcatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg ggttttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctctttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgatggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatt cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAEBXD010000236         3112 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEBXD010000236 DAEBXD010000000
VERSION     DAEBXD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.241"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="KJL94_004174"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="KJL94_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBD4412278.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="KJL94_004175"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="KJL94_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="KJL94_004176"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="KJL94_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBD4412279.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="KJL94_004177"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="KJL94_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBD4412280.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="KJL94_004178"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="KJL94_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          772 a          754 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcaccgctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAEBXD010000237         3097 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEBXD010000237 DAEBXD010000000
VERSION     DAEBXD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.242"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(104..1813)
                     /locus_tag="KJL94_004179"
     CDS             complement(104..1813)
                     /locus_tag="KJL94_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBD4412281.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGKVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="KJL94_004180"
     CDS             complement(2245..2964)
                     /locus_tag="KJL94_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBD4412282.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          952 a          587 c          518 g         1040 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata acttttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatt ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgatcatggt gggaaggctt agaacgc
//
LOCUS       DAEBXD010000238         3066 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEBXD010000238 DAEBXD010000000
VERSION     DAEBXD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.243"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="KJL94_004181"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="KJL94_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBD4412283.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="KJL94_004182"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="KJL94_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBD4412284.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3066)
                     /locus_tag="KJL94_004183"
     CDS             complement(2711..>3066)
                     /locus_tag="KJL94_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD4412285.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          721 a          678 c          753 g          914 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gataat
//
LOCUS       DAEBXD010000239         3021 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEBXD010000239 DAEBXD010000000
VERSION     DAEBXD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.244"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..60)
                     /locus_tag="KJL94_004184"
     CDS             complement(<1..60)
                     /locus_tag="KJL94_004184"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412286.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="KJL94_004185"
     CDS             171..1661
                     /locus_tag="KJL94_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD4412287.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="KJL94_004186"
     CDS             1713..2870
                     /locus_tag="KJL94_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD4412288.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="KJL94_004187"
     CDS             complement(2836..2958)
                     /locus_tag="KJL94_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412289.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          921 a          663 c          611 g          826 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc t
//
LOCUS       DAEBXD010000240         3002 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEBXD010000240 DAEBXD010000000
VERSION     DAEBXD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.245"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1216..1818
                     /gene="fimB"
                     /locus_tag="KJL94_004188"
                     /pseudo
     CDS             1216..1818
                     /gene="fimB"
                     /locus_tag="KJL94_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            2296..2892
                     /gene="fimE"
                     /locus_tag="KJL94_004189"
     CDS             2296..2892
                     /gene="fimE"
                     /locus_tag="KJL94_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBD4412290.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          912 a          529 c          608 g          953 t
ORIGIN      
        1 tattcgtaag ttatatcact tactcaaccc attttaccca gagtcataaa atagagtgtc
       61 tgttgatctc attttcatat tcctttcaga ctgaagtgca ttttcatcac ccgatgtgaa
      121 agaactgaag tgatgtttgc catagattac gagatgccca gtctcattga ctatcgtggt
      181 gcatctttat acctgttata ccagatcaaa aatcacgcaa tccatacaac aaaaccagat
      241 ttgcaattcg cgtcacaaaa tatgtcgatc tttttctaag aagaagatgc catgtgaagc
      301 cagacgaaca cttgcggtgg tcttcaaaaa ctaaagattt tcttttaact atatgtttta
      361 taaataattt atttagaatc taaacaaaga aagctttgca agctaactca gtgagagtgg
      421 tgaaaataag ggtttactcg ccatcaggct gagcataatt ctcatcatga aatatgtttc
      481 ttggtttgtg gcttgtaact ggtcactcct gatgtcgatc tggagaggct tgttgatgtt
      541 ggtgttttca ggatgatgtt tcacttagtt tgtttgccgt atcgcccggc gaatagctgt
      601 gattgaggaa ggcttaagtc gtagtgacca aagctatatt taccaacgga tgtagatgaa
      661 aaagcgtcgc ctgcgttccc ccatatctcc aggataaaaa ggaatgtaac aatctcatgg
      721 cctaagctga cgaaccaggc aggaataatc gctagggatc taagaattag catgataata
      781 gccactaaga aattactgcg ttccatgaaa tagccatttt gtggcaaatg cagttaacta
      841 ataatgtcat atttgagacg gctagtttaa caaatattaa attttgctga attttttatg
      901 ttgattttac ttgttacaga acatatcaca tgatatatag ataagattag ttgcattaat
      961 gatgagggtt attattagat tcgtatcaga ttgataaata tataaaggta catagcatgc
     1021 aagagcatgg cgtttgtatg gcaaagttat tataagtaac tatcgctact ccatttaagt
     1081 tcgctcagaa gaactggtcc acttacgtaa gttattaagc aaacgttcgc ttttataaac
     1141 ataatcagga taaaaatgtt ggattattgc taacccagca cagctagtgc gcgtctgtaa
     1201 ttataaggga aaacgatgag gaataaggct gataacaaaa aaaggaactt ccttacccat
     1261 agtgaaatcg agtcactcct taaagcagca aataccgggc ctcatgcaac acgtaattat
     1321 tgtctgattt tgctttgttt tattcatggt ttccgggcga gtgaaatttg tcgattgagg
     1381 atttcggata ttgatcttaa agcaaagtgt atatatatcc atcgattaaa aaaaggcttt
     1441 tcaacaacgc acccgctatt gaacaaagaa gttcaggctt taaaaaactg gttgagtatc
     1501 cgtacttctt atccgcatgc tgagagcgag tgggtatttt tatcacgtaa ggggaatccg
     1561 ctttctcggc aacagtttta ccatattatc tcgacttctg gtggtaatgc cgggttgtca
     1621 ctggagattc atccgcacat gttacgctat tcgtgtggtt ttgctttggc gaatatggga
     1681 atagatacgc gacttatcta ggattatctt gggcatcgca atattcgtca tactgtctgg
     1741 tataccgcca gcaatgcagg gcgtttttac ggcatctggg atagagccag aggacgacag
     1801 cgtcatgctg ttttatagag atagttgaga taccagggat ggtgttaaaa actacattat
     1861 tcttacgata tatcaaaaat gattaaccat ttattaattg atgttattgt aagtgtgatt
     1921 cttaattatc ataataaaca ttaagttaac catacccata caaaatctaa tggtttatgt
     1981 tcttcaaaat aaataaacaa aatcattcat aagtttacac atcacttaaa ttctcctgtt
     2041 tccacacttt tttctttatt ttttaagtaa ctggaagtta atcctctgta atctattgtt
     2101 atattgaatc aaatcaatga aaatagatgt tgtcacatca gtgatatttt atttttgtat
     2161 gatgtttaat gtaattgact gatagccaca taacttcgtg tgtggttatc tttttatcta
     2221 ttgggctaat tttgaccgat tgaggtttcc tataggtatt cattcaaata tatctcagtt
     2281 aggagtacta ctattgtgag taaacgtcgt tatcttaccg gtaaagaagt tcaggccatg
     2341 atgcaggcgg tttgttacgg ggcaacggga gccagagatt attgtcttat tctgttggca
     2401 tatcggcatg ggatgcgtat tagtgaactg cttgatctgc attatcagga ccttgacctt
     2461 aatgaaggtc gaataaatat tcgccgactg aagaacggat tttctaccgt tcacccgtta
     2521 cgttttgatg agcgtgaagc cgtggaacgc tggacccagg aacgtgctaa ctggaaaggc
     2581 gctgaccgga ccgacgctat atttatttct cgccgcggga gtcggctttc tcgccagcag
     2641 gcctatcgca ttattcgcga tgccggtatt gaagctggaa ccgtaacgca gactcatcct
     2701 catatgttaa ggcatgcttg cggttatgaa ctggcggagc gtggtgcaga tacccgttta
     2761 attcaggatt atctcgggaa tcgaaatatt cgccatactg tgcgttatac cgccagtaat
     2821 gctgctcgtt ttgccggatt atgggaaaga aataatctca taaacgaaaa attaaaaaga
     2881 gaagaggttt gatttaactt attgataata aatttaaaaa acaaataaac acaagacaat
     2941 tggggccaaa ctgtccatat cataaataag ttacgtattt tttctcaagc ataaaaatat
     3001 ta
//
LOCUS       DAEBXD010000241         2951 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEBXD010000241 DAEBXD010000000
VERSION     DAEBXD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.246"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="KJL94_004190"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="KJL94_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBD4412291.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="KJL94_004191"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="KJL94_004191"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBD4412292.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="KJL94_004192"
     CDS             2603..>2951
                     /locus_tag="KJL94_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD4412293.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAEBXD010000242         2924 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEBXD010000242 DAEBXD010000000
VERSION     DAEBXD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.248"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            69..437
                     /locus_tag="KJL94_004193"
                     /pseudo
     CDS             69..437
                     /locus_tag="KJL94_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            699..1061
                     /locus_tag="KJL94_004194"
     CDS             699..1061
                     /locus_tag="KJL94_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBD4412294.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1058..1978
                     /locus_tag="KJL94_004195"
     CDS             1058..1978
                     /locus_tag="KJL94_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD4412295.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1981..2924
                     /locus_tag="KJL94_004196"
                     /pseudo
     CDS             1981..2924
                     /locus_tag="KJL94_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
BASE COUNT          839 a          579 c          589 g          917 t
ORIGIN      
        1 ccttcaggga aaaatatcat cagatggctg cttaaaaaaa gaacaaatgg tagtgtccgc
       61 tattgccagt acacctcagg gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa
      121 taaaaacagc agcggtaagg aagatgagaa ccgacagcaa caaggcatgg aagggagcgt
      181 taaagcgggc aggcattagc aacttccgct tccatgacct gaggcatacc tgggcaagct
      241 ggctggttca gtccggtgtc tctcttcttg cgcttaaaga gatgggagga tgggaaactc
      301 tcgaaatggt tcaaagatac gcccaccttt cagccgggca tctcaccgag cacgcgagca
      361 aaatcgatgc gattataagt cgcaatggca caaatacggc acaagaggag aacgtagttt
      421 acttaaatgc gaggtaactt attgatttaa atggtgccga taataggagt cgaacctacg
      481 accttcgcat tacgaattat aagaatccgc ttgtaattca aaggattatc ccatcaacac
      541 tgcgctcaca cgtcccacca catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct
      601 tatgtgtctc agttttgtct catcagacat agcaagtatc gatcaattga gacttggatg
      661 atagacttca tgcctttcag agctcattga ttaaataaat gttaaagtta tttgtaaagt
      721 acacatctat aggtgtgctg aacacactta tacactgggt ggtttttggt gtatgtatct
      781 ataccgcaca taccagtcag gctctggcaa acttcacagg tttcgtagtg gctgtgagct
      841 ttagcttctt cgcgaatgca agattcacat tcaaagcatc gactacagcg atgcgctaca
      901 tgttatacgt gggatttatg ggaatattga gtgtgattgt tggttgggca gctgataaat
      961 gctcacttcc tccaatagtc actcttatca ccttctccgc catcagtctg gtgtgcggtt
     1021 tcgtctattc aaagttcatt gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc
     1081 tgtcttcaat gaagaagaag cgatacctgt tttctataaa acggtacgtg aattccaaga
     1141 gttgaagcca tatgaagtag aaattgtatt cataaatgac ggaagtaaag atgccacaga
     1201 gtcaattatt aacgcgctgg ctgtttcaga cccgctagtt gttccgctgt catttacacg
     1261 caactttggt aaagaaccag ccttatttgc agggttagac catgcaagcg gcgatgctgt
     1321 aattcctatt gatgtcgacc tgcaagaccc aattgaggtt atccctcatc ttattgaaaa
     1381 gtggcaggca ggtgctgaga tggtgcttgc taaacgttca gaccgctcaa ctgatggacg
     1441 actgaaacgt aagacagctg agtggttcta taaattacac aacaaaataa gcaccccaaa
     1501 gatcgaggaa aatgtcggag attttcgact catgtctcgt gaggttgtgg agaacattaa
     1561 actgttgcct gagcgcaatc ttttcatgaa aggcatactg agctgggtgg gtggtcagac
     1621 ggatgtcgtt gaatatgtac gcgcagagcg tgttgctggc atctcaaaat ttaatggctg
     1681 gaaattatgg aatctggcac tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt
     1741 atggacttat ataggcttgt ttgttgcaag catttcattt ttatatggtg catggatgat
     1801 tatagacacc cttgtctttg gtaacccagt acgcgggtat ccctccctgc ttgtatcaat
     1861 acttttcttg ggtggagtgc aactgatcgg gattggtgtt ctcggtgaat atataggtag
     1921 aatttactta gaaacaaaat caagacctaa gtatattttg aagaaaggta gaaaataacc
     1981 atgagcatat gtattaaaca atcagcatta aaaatattat tggctttatc agcactccta
     2041 ataacttggt tgactaccag atactttcca gttgaaccgg atgttgctaa cagcccaatt
     2101 gtatggaggc atattttaga gaatggtata tcatcaatcc acgactggaa accaaccgtg
     2161 gataattggt atttcaccgt ttacccaatt aactttttat tttatatgct gttaggtgat
     2221 gatggcttag tagcattaag attatcaact gcagttttct ctattgctat agtaatagca
     2281 gcgatgttaa cgctacgtaa ggcattcggg tttacccctg ctttattttc tattatactt
     2341 ctatctctaa taccatattt ttcatacact tatggttttg tatcacatcc tttttctcat
     2401 aattcaacaa acgctttcgg ctttttatgc ttgcttatat ctgttttcaa catacagtat
     2461 aaaaatatat ttatcactct cctcttaagt ttgacagcat tattttccag cgtatccgat
     2521 ccgtggttta cagctgcatt ttttattcca ctattaatat cttacttttt attctctgta
     2581 tgggataaaa aattatttaa gcatactgca ttaatactat ttgcatgttt aatctctctt
     2641 tccaatgtat tgcaaaatct acttaacata cctccccacc aatttgaaat tgtatcactc
     2701 aatgatatga ttttaaatgc aaaatggtgc attctgttaa taggaaaaag tcttaattta
     2761 ttagttgtcg acaataacgc aacatcttac gcctcatttg tcatttggtt cattgccatt
     2821 attacatctg cgtggtttgt tttatcggac aataaaaaaa acacataccg tatttatatt
     2881 gtgttatttt cattattatc tatcgcaggt attgtctcat catt
//
LOCUS       DAEBXD010000243         2917 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEBXD010000243 DAEBXD010000000
VERSION     DAEBXD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.249"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="KJL94_004197"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="KJL94_004197"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBD4412296.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="KJL94_004198"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="KJL94_004198"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBD4412297.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="KJL94_004199"
     tRNA            2657..2732
                     /locus_tag="KJL94_004199"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..>2917)
                     /locus_tag="KJL94_004200"
     CDS             complement(2747..>2917)
                     /locus_tag="KJL94_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4412298.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNL
                     SQSKNKEGTNDV"
BASE COUNT          700 a          729 c          821 g          667 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac cattaaaatc aataagttac acatcattag
     2761 taccttcctt attttttgac tgggacaaat ttgggaccga tgggttcagg atcgagtcta
     2821 tttgccgtgc gtgttcggta aggtgattag gtgcaaggtg agcatatcga cgaaccattt
     2881 cgatagactc ccagcctccc atttcctgta acactga
//
LOCUS       DAEBXD010000244         2890 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEBXD010000244 DAEBXD010000000
VERSION     DAEBXD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.250"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="KJL94_004201"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="KJL94_004201"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBD4412299.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="KJL94_004202"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="KJL94_004202"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBD4412300.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="KJL94_004203"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="KJL94_004203"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBD4412301.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="KJL94_004204"
     CDS             complement(1896..2810)
                     /locus_tag="KJL94_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBD4412302.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          758 a          668 c          772 g          692 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagctcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg ttcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct
//
LOCUS       DAEBXD010000245         2808 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEBXD010000245 DAEBXD010000000
VERSION     DAEBXD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.251"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            6..81
                     /locus_tag="KJL94_004205"
     tRNA            6..81
                     /locus_tag="KJL94_004205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KJL94_004206"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KJL94_004206"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KJL94_004207"
     CDS             complement(299..1057)
                     /locus_tag="KJL94_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBD4412303.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KJL94_004208"
     CDS             complement(1065..2168)
                     /locus_tag="KJL94_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBD4412304.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2808)
                     /locus_tag="KJL94_004209"
     CDS             complement(2178..>2808)
                     /locus_tag="KJL94_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBD4412305.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          739 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaac
//
LOCUS       DAEBXD010000246         2800 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEBXD010000246 DAEBXD010000000
VERSION     DAEBXD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.252"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..523)
                     /locus_tag="KJL94_004210"
                     /pseudo
     CDS             complement(1..523)
                     /locus_tag="KJL94_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSfl11 family transposase"
     gene            complement(861..2315)
                     /gene="ospC4"
                     /locus_tag="KJL94_004211"
     CDS             complement(861..2315)
                     /gene="ospC4"
                     /locus_tag="KJL94_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBD4412306.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKVN
                     SGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          775 a          560 c          508 g          957 t
ORIGIN      
        1 ccgacgataa ttccgttatc tgccagctcc gtctggagcc gccgggttcc atatgtttcg
       61 cgagtgcgga tatgtgccac cttaatctcc agttttagcc gctcatcact ttgttttctg
      121 tctgaggggg catgctgtac ccggtcgtaa taaccgctcc tggatacacc aggtacccga
      181 cacatcgctt caacagggaa ttgttgtcgc cattgttcga ctaacgcgta tttttcagcg
      241 actcctgtgc aaaatacgct gttgcttttt ttaaaatatc tcgctcaagt cgtgcttcat
      301 ttaaagcctt acgcagttgc ataacttcag attccagctc agccactgtg cgggagcctg
      361 gtgtattgag cccttttctt gcggcggtaa cccattgtcc taaagtgcct tcaggaacag
      421 ataatcggga agcgccttca ctgatcgaaa gctgattttc aggaaccgtt ttgacggctt
      481 cggctttaaa ctccgtagag taacgttggg tttttctgct cattattggc tccttctgat
      541 gccattctat ttcaggaagg agtgtccgtt aaactcaggc tacctcactc ccgtaaagac
      601 aggagtcaag ttcccgctcc gacataactg cgtgcaatgg attttgaagg aaccgtcagc
      661 ccattccccg gaggcaaaca tgggctggca aaagtgtagc ggtattaggc acagctattt
      721 aggaaagagg gaccatctcc atctcattaa atggaccacg ccagacaatt cactgtcatg
      781 ctgatgctgc actctgggga cagaatcact catgatgact tcgataatcg acgacattat
      841 tatttggccg agcttttagg tcatatctca aatcgtttgc ctaatattgc tccatttttt
      901 aataaaaaat caatcatttt tgaatctttg cttttcattg cattgtcaag catagtgtcc
      961 ccactattta ctttttgaaa ttttttgttt acatcaacca atccattatt gataaaatat
     1021 tctagcactt tatagttggc accatcttta ctaagtaagt attcaacatc atacagatta
     1081 caatatctat tgtttttcat tttctccatc tctgcgacat cttgttttgt aaaattaaat
     1141 ttagatagta aataaagtgc tatatcagct tttttattgc ctaaagaata agcaagggcc
     1201 tgatgagcca tttttttatc agtcacttta ttgttaatct cttcataatt agatgcttta
     1261 atcgcatcct ccaaagatat gtctctgagt tttatttttt tgaagttatc tgtacttagc
     1321 attcttggta tatgatactc aagctgaaac acaaagttta taattctatc cagactaaca
     1381 ggatcaatat ttttgttata acaaaactct ctgaatcctt gatctttact ctttcttata
     1441 aaatcaataa gatgcaaccc aattccaagc ctcatatctt tagtgttaaa tattggaaca
     1501 tcagttttcc cctgattacc atgcacatac ctgctaactt catccacaac ctctttaaag
     1561 ttatctaaga aaaatgattg gtgctcatgg taaaaaacag gtggaatagc attatcaaag
     1621 tgatcggtta atgtcatata tccatatgga gagtcatgat caatgatata cgcattggcg
     1681 ccaaaatcta cagtatgatg tgttttattt agtgggagtt tttcttgatg attggaaatt
     1741 tcgaccccaa aaaaaacaaa atcgtggtta gagatttctt taatatcctc agagtatgta
     1801 ttcttatgtg tttgtatatt ttcagactga attttatcaa aggatttaat attaagaata
     1861 ttatttgaca gattagagtt actttgatgt ctgaagttaa aattagaatt aataataata
     1921 gaaagtaagt gacgttcgtc tgaagaaaga ggatgaactt tttgtattat ctcagagata
     1981 tagtgcctgg acaccttatc gagatgctca agtgaccgaa atgatgaccg tgctccagat
     2041 ggcgcttcaa tagaaagatt tagtgatttg aaactggtcc cttgagaaaa cattttgcta
     2101 taactttgcg ctgctataga cttccttaaa aagtttttca cagtattagc acagtgatct
     2161 aaatgttttt cacttatgcc agaagaagat gagtttgtta cgcgtaatac atttttttgt
     2221 aatgcttttg ttgctttatt cggattcgtt tttccatcaa caagatcaat attattctga
     2281 acattaatat gatttactgc ttcaggtatt ttcatccccc cccccaatta tgtgcgtttt
     2341 tatctgtggt tatttagata acattatcta actggacact ttcttgtatg tattatagct
     2401 aaatgaacag attttaatat gttgtatggt tttcactctg aaatcccctg aatttaatca
     2461 cgtttccttt actgaaggca attttaaccg tagtatcaat tattaaccct tgggcttgat
     2521 atccctctcc tcttctgggc acgggggatg tcagattact tattacagaa accaaaagaa
     2581 ttgtaagcgt catctcagtg tcgtctattt caatgtctga tgattggcga tgatagagtc
     2641 ctcttataac gttaatggac tctatcaatg tcagattctc ttcgcccccg caggattcgt
     2701 gcctcttact ctatgactct atggagttta agcttgatct tattgaaaaa tcttaccagc
     2761 taggggcctg cgtggcccaa ctggccagag agtacgggat
//
LOCUS       DAEBXD010000247         2690 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEBXD010000247 DAEBXD010000000
VERSION     DAEBXD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.253"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            230..769
                     /locus_tag="KJL94_004212"
     CDS             230..769
                     /locus_tag="KJL94_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBD4412307.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KJL94_004213"
     CDS             complement(995..1318)
                     /locus_tag="KJL94_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBD4412308.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KJL94_004214"
     CDS             1604..1798
                     /locus_tag="KJL94_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD4412309.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAEBXD010000248         2664 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEBXD010000248 DAEBXD010000000
VERSION     DAEBXD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.254"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..1115)
                     /locus_tag="KJL94_004215"
     CDS             complement(<1..1115)
                     /locus_tag="KJL94_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4412310.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDT
                     VSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADAT"
     gene            complement(1715..1963)
                     /locus_tag="KJL94_004216"
     CDS             complement(1715..1963)
                     /locus_tag="KJL94_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD4412311.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2318..>2664)
                     /locus_tag="KJL94_004217"
     CDS             complement(2318..>2664)
                     /locus_tag="KJL94_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD4412312.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          681 a          502 c          663 g          818 t
ORIGIN      
        1 gtggcatcag cagcaacagc gaaagactgc tgtcgaagct ccgcagaggc actgagtttt
       61 tccagccatg cagcgacctg ttcacggaat ccggaggtat tgcgtgcaga gacggtatcg
      121 gaaaggcggt caaggaacgc ggaaaaggtg ttggcgtgct cttcatgttc aaaagcatgc
      181 catatctgtg atacatcaga ttgtttgttt tccgggaacc atgctgtcac ggcatcagcc
      241 agggggcgat ggagtgtatt ctgttgtccg tcactcatgg agaagtaaat ctgcgggccg
      301 tggtagtccg gcgaagatgt taatctttgc agggattgca gagcatggga tgacaatggg
      361 ttatcactaa tatctattga acattcattc ctcagattaa gaatgctttc cgggatatga
      421 cttatctgat ttatgtcaaa ataaccgttc cttaaatttt ccggcaggta tggaatatcg
      481 gttaacatat tgtcgattgc ccatatggag tgaatgttgt gaggtagagg ggggagttca
      541 ctaagaagat tccctattgc gctaatctct tgtaaagaaa atggtaaagg tggtaaaact
      601 gccagcccat tacatgatac atctaatgtt tccagtagtt ctggtaaaac aggaagagaa
      661 cataattgat tatttgagac atgaagctcc ttcaaagata tggggagtgt gggtagtgtg
      721 attagttgat tgtgggacgc attcaataat ttaagtcctt gaggcaaagc aggcagttca
      781 ataagtctgt tatagctgac attaagttgt gtaagggacg caggtaatgg ggagattaag
      841 cttaaattat ttttacttat attaattgat ttaattcccg gggggatttc aggtaatgtt
      901 gtcaggccta attcagacaa gtccaggttc gtctcttggt tttgtagaca tgatactagt
      961 cgctgaaaag cgatgtctcg ttgttcttct tgtatgcggt tatttttcca ttcagtccaa
     1021 tgggttaggt aattttcata tgcgcggcta gtgtcgattg aataagtgga gaacgaattt
     1081 gaaattaatc tgtgattgtt atttgtcggg agcataaata tcagggcgta ttctttatgt
     1141 tgagaggtgt tggattcttt tttatctgct ttgcaatgtt attggttccc ttcagcagta
     1201 gacaggaact tctggaggag gcggatatat agtttgtgta agcatattaa ctccatatgt
     1261 tatatattga agaacttctg ctttacacta tcctaaactg gatcggttta atgtaaaaaa
     1321 acgatacttt ttgggggggg ggcaattttt ccttaaggta tgaaaaaagg agctgaagct
     1381 atataatagc tattaatgcc actggtatca gtgaatcatg tatataaaaa actgtgttaa
     1441 ggaggctgtc cattagcggt tttttattct tctggaaccg cggcatcttt gcgtatcttt
     1501 ttgtcttctc accgtctggt cggtgttttg ctgagactgc taatttcctg tctttgctag
     1561 tgctgtactg gtactgccca atcatgattg gcgggggcgg gcggagttga gaccgcagac
     1621 acgtcgtatg caagaacgtg ctgcggctgg ctggtgaact tccgatagtg cgagtattga
     1681 atgatttcca gccgttactg attttacgtg ctaattagtg aacaaaccac tcgtcagcag
     1741 actcccaggt atctttcaga gtttcctgaa caaaagtttt agctgaatct ttatcggcgg
     1801 tgcgcgtaac agaaaggcca tcgttgctgg tggcttttac gatcacctct acatcgtcat
     1861 aacgtttact gatgcgtcgg gttaattctt cctttaacgc atccacagca ccggttggca
     1921 ttttagtcat tttctctttg gctatgcaga tttcaaaacg cataaaaatc cctctgcact
     1981 gtgtttttat acagtattat ttttaactgt atggataaac agtgtcaaga gtgagggaaa
     2041 gtgatttttt ggggcatgca tggggcaaaa atatttgttg tggggcattt tggggcatga
     2101 ttgggacact ttgttacata tgaactttgc cgattttcat atgacatggc aatccaacgc
     2161 tttgattatt ctaaagaaaa cacatgctct tgggcgttct ttagtgattt ttaaaaatcc
     2221 cgtatcacgc agttaaagtg gcgggcatac tcttcaaggc tggtgatccc caaccgcacc
     2281 catttaggat gtgaccattg cggtagaccg atgtaaatca taacgcgtga aggatctctt
     2341 ccacgctacg cacacgggcg atgcgcgggt agatatgatt aatgctgtta ttgtgttgct
     2401 cggcgctggc ggcactacag gcgtcttcgg caatcaccag attaaagcca agttcccagg
     2461 cattgcgggc ggtggattca acaccgatat tggtcgagat cccacataac actattgtat
     2521 cgatacctcg gcggcgcagt tgcaactcca gatccgtacc gtaaaacgca ccccactgac
     2581 gtttgatgat ttcgatatca ctgtcagttg cacctaatgc agctggatgt tgccaccagt
     2641 tttcgggcaa cactttagcg gggg
//
LOCUS       DAEBXD010000249         2644 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEBXD010000249 DAEBXD010000000
VERSION     DAEBXD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.255"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..570
                     /locus_tag="KJL94_004218"
     CDS             <1..570
                     /locus_tag="KJL94_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD4412313.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="KJL94_004219"
     CDS             571..936
                     /locus_tag="KJL94_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD4412314.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="KJL94_004220"
     CDS             933..1616
                     /locus_tag="KJL94_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD4412315.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="KJL94_004221"
     tRNA            1815..1890
                     /locus_tag="KJL94_004221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="KJL94_004222"
     tRNA            1892..1968
                     /locus_tag="KJL94_004222"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="KJL94_004223"
     tRNA            1971..2046
                     /locus_tag="KJL94_004223"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="KJL94_004224"
     tRNA            2050..2124
                     /locus_tag="KJL94_004224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="KJL94_004225"
     CDS             2417..2632
                     /locus_tag="KJL94_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD4412316.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          691 a          581 c          715 g          657 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcat
//
LOCUS       DAEBXD010000250         2592 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEBXD010000250 DAEBXD010000000
VERSION     DAEBXD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.256"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..213)
                     /locus_tag="KJL94_004226"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="KJL94_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            488..792
                     /locus_tag="KJL94_004227"
                     /pseudo
     CDS             488..792
                     /locus_tag="KJL94_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            961..2325
                     /locus_tag="KJL94_004228"
     CDS             961..2325
                     /locus_tag="KJL94_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4412317.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSRPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2375..2584
                     /locus_tag="KJL94_004229"
                     /pseudo
     CDS             2375..2584
                     /locus_tag="KJL94_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          669 a          526 c          607 g          790 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacaggact ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agttgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cgcccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 gggggggttt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca
     2161 ttttcctata tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat
     2221 ctgggatggg gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc
     2281 acactgatga gtcgtgaacg ctcactggag ttcgaaaaag agtaattttc tcaggaaaaa
     2341 cataaaatta ctgactaaat atttattttt tgaatcgcag ggcattccgg ataaggttga
     2401 tatcgtgtca cggctgaaga cctatggata cagtatttct ggtgtggaga cggatgacgg
     2461 atataaggcg cttgtccgcg cttttcagct tcatttccgg cagaaaaatt atgacggcat
     2521 aatggatgct gaaaccgctg ccatcctgta tgcattgctg gagaagtatt tcccgggtaa
     2581 ataaggagtg ag
//
LOCUS       DAEBXD010000251         2591 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEBXD010000251 DAEBXD010000000
VERSION     DAEBXD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.257"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            147..398
                     /locus_tag="KJL94_004230"
     CDS             147..398
                     /locus_tag="KJL94_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4412318.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            400..2496
                     /locus_tag="KJL94_004231"
     CDS             400..2496
                     /locus_tag="KJL94_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412319.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT          731 a          600 c          707 g          553 t
ORIGIN      
        1 taattcattg ataaataata aaattaaaaa ataaaccata aaaacaccaa agaacccttt
       61 acagggaatc tttggtatct taatctttat ccatacggat atccgaccgg atatccgcgc
      121 gggtaatttt gtaggttaaa ggaaagatga gacaaagaga agatagtgac cgcgattggc
      181 cttcgctgaa gattcagctg gcgctcaaag ataacgggtg gtcttccttc cgggagcttt
      241 cactccatgc tggttatgct caggattcac tgaaagcggt agttcgcaca ccaaacaaaa
      301 aatacgaacg cgtaatttct gacgctatag gtgtaccgcc agaaatcatc tggcccagcc
      361 ggtatcgtaa cgatggattt atcaggaagg cgtcgtgata tgcagtcgta cgttaccgta
      421 aatgacctgc tcggcgtacc aggaatgcca gccaccacaa aaggcatccg acaggcgtta
      481 cagcgttttt ctcgtgatct tggtgacgtt agtcgccgtc gggaaggcac aaaagccatt
      541 gagtaccaca ttgattgtct gcctgaaata acacgaaagg cactgcgtga acgctatgtg
      601 gaacagctgg tggctacaga gaacaatgtt tctgaagtga aagctgttac cagaaaaaca
      661 cgcaatcctg acgctgtcca ggcaatcgaa gcatatcgcg gttctccaca actgatggaa
      721 gaacgcctga atgcgctgac cgaaaaccag cgctgggtat ctgaagcaag agctgcgctg
      781 gtggtggaag tgctgaagct ggaaagcgcc ggtaaccccg ggcgactgaa agccattaac
      841 tttcttgttg aaaaagcccg taaaggtgag ctgccggagc gcctgcaaca ggccgcagtt
      901 aacgccaatg caaaacgtgg cgctaatcgt actatcagcc gcgatccact ttaccagtgg
      961 gttctgaaat acaaccagtc gcagaacgcc gccgaacggc tgctgttact ggcaccggga
     1021 aaacgcgacg aaataaaacc ggaagaaatc agctggctgc ctgaatttct ggcgcagtat
     1081 cgccaggtga acggcagacc catgtcagaa gcctacgagg attttgtcgc agaatggcag
     1141 cgtcgccatg cggacgaacc gtacatgctg gaggtgatgc cttcctatga tgttgtccgg
     1201 tatgccatga aaaaactgcc ggaagtggtg aaacaaaaag ggcgcgttac cggcagtgaa
     1261 taccgtcagc ttgaaggatt cacccgccgc gactggacag ctatgccggt gaattatgtc
     1321 tggattggtg acggtcacgg tatgaagctg aaatgtgcgc atccgatcca cgggcgtcca
     1381 ttctcaccgg aagtcacgtt tgtgatcgat ggtggaacac ggtttgttgt tggctggagt
     1441 cttgaccttg ccgaaaacgt gttcgccgtt gccggtgcga tacagcacgg cattcgcaac
     1501 cacggaaaac cgttcctgta ttactcggat aacggttccg gtgaaacggc tgacatgctg
     1561 gacaaagagg tcgtgggtat tctgccacgc ctggggatta aacaccctac cggtattgcc
     1621 ggtaatccac agggacgcgg cattattgaa cgcctgaacc ggacactgcc gatgcgtata
     1681 gccaggaggt atcgcaccta ttttggcaag ggtgctgacc gggaatcgtt gcgtgtactt
     1741 aaccgtgatc tgcgttcagc gtttaacgcc ctgcaacagg acaagcctct gaacgaccgc
     1801 cagaaagcgg caatgcgcga gctgccatcg tgggcggagc ttattgaggc tattcgtgaa
     1861 ggcgtggaat ggtacaacaa ccgcccgcat tctgagctgc cgatgaaacc gaacggacgg
     1921 cattacagcc cgacagagtt cagaaaaaaa cgccaggcag aagaagacac cgaaattgag
     1981 tggctgagtg atcttgagct gcgcgacatg ttccgcccaa tggtggaacg accggtaaga
     2041 cgctgcgaaa ttcagtggct gaacaatatt tactacgcgc cggagttgcg tgacgaacac
     2101 ggtcgcaaag tgcttatcag ttatgacatt catgacgccg agcgaattac ggtacgtcgc
     2161 aaggacggca gctttatctg tgaggcgata tggaacggca ataaacgcgc agcctttgct
     2221 gtcagtgctg aatatcacaa acaacagcag cgtattaaag gaatgcgcaa acgcgctgag
     2281 gaaaaaattc gtgatgctga agatgaaggt attcagattc tggaacacaa acaggctgaa
     2341 ccctggctga gtaatgttta tcgcccggtt ggtaatgtgg tggcagttca gcaaccggaa
     2401 tatgaagaag agcatgacga agaatttgaa cgcgatttcc ggctggggat gcaaaaactt
     2461 tttgccatgc aggaagaaga tgatccgctg gcctgaataa aaaaacagtc cgcgggaacg
     2521 gactgttaaa taaaggaaac aaagctgatt tgaaataacc gaggaaatac tatgactgat
     2581 attgatgatg t
//
LOCUS       DAEBXD010000252         2567 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEBXD010000252 DAEBXD010000000
VERSION     DAEBXD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.258"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="KJL94_004232"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="KJL94_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBD4412320.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="KJL94_004233"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="KJL94_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBD4412321.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="KJL94_004234"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="KJL94_004234"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD4412322.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          741 a          489 c          582 g          755 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg attatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaacc
//
LOCUS       DAEBXD010000253         2516 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEBXD010000253 DAEBXD010000000
VERSION     DAEBXD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.259"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..671
                     /gene="ospC1"
                     /locus_tag="KJL94_004235"
     CDS             <1..671
                     /gene="ospC1"
                     /locus_tag="KJL94_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBD4412323.1"
                     /translation="EVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIRKSED
                     QSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAVTASN
                     YEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDIEYLL
                     SAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYGATSD
                     NKYI"
     gene            complement(775..924)
                     /locus_tag="KJL94_004236"
                     /pseudo
     CDS             complement(775..924)
                     /locus_tag="KJL94_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1000..>2516
                     /gene="ospD3"
                     /locus_tag="KJL94_004237"
     CDS             1000..>2516
                     /gene="ospD3"
                     /locus_tag="KJL94_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBD4412324.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAA"
BASE COUNT          874 a          429 c          482 g          731 t
ORIGIN      
        1 cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac
       61 caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta
      121 gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg
      181 atctggatag aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa
      241 gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag
      301 catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc
      361 tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg
      421 agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac
      481 ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg
      541 atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa
      601 aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata
      661 aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg
      721 atatattgtt tattgtcagt atggctcaat gtgataatag ctggaaagtt tgatgggttt
      781 cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag
      841 cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg
      901 acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa
      961 gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc
     1021 ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca
     1081 atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta
     1141 ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta
     1201 gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt
     1261 gcttttccgg atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat
     1321 gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt
     1381 ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat
     1441 cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt
     1501 cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac
     1561 ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt
     1621 tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg
     1681 tcctccataa tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg
     1741 cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt
     1801 tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg
     1861 cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa
     1921 acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga
     1981 cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac
     2041 ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg
     2101 accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa
     2161 aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc
     2221 aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt
     2281 ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat
     2341 gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt
     2401 ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg
     2461 aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact ggcagc
//
LOCUS       DAEBXD010000254         2510 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEBXD010000254 DAEBXD010000000
VERSION     DAEBXD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.260"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..178)
                     /locus_tag="KJL94_004238"
     CDS             complement(<1..178)
                     /locus_tag="KJL94_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD4412325.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(381..848)
                     /locus_tag="KJL94_004239"
     CDS             complement(381..848)
                     /locus_tag="KJL94_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD4412326.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(845..1378)
                     /locus_tag="KJL94_004240"
     CDS             complement(845..1378)
                     /locus_tag="KJL94_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD4412327.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1444..2073)
                     /locus_tag="KJL94_004241"
     CDS             complement(1444..2073)
                     /locus_tag="KJL94_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412328.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2077..2292)
                     /locus_tag="KJL94_004242"
     CDS             complement(2077..2292)
                     /locus_tag="KJL94_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD4412329.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          630 a          657 c          565 g          658 t
ORIGIN      
        1 ccctgggtac tttcttcatg aaactgcggt gttcgagttt gcgatatttc ttacagggga
       61 acgcaagccc ctccgcctcc gctttggatt tccgatcagc attaatgtaa tccaccatct
      121 caagactgct catggtcggg tattcgccag aaggaagaac ggttaatgat ttgttcatgt
      181 cggtatttcc tttagaaagt tgagcctgtt cgcacagaaa agccgtcccc gagaggtcct
      241 cacctatacg gcagttctca ggctcagctt tctgaaagac tcgggattgt tacgcgctgc
      301 gatgcgcggt ttactgcgga catataaaag ccccgcaagt gcgaggctca ttaaatggac
      361 tttgtgattt gcaaaaaaat ttatttcagg cactgagtcc tgatgtactc ctgaagcatt
      421 ctcaatgctg tctggtcgct gatgattccg tcccggatac cgagaacgtt tcgtccagca
      481 actggagaga gttcgacggt ggcatcattg cccatgccgg aggcgctgga ggtttcggct
      541 gaggatggca cagggcattt tcctttgacg agcacccgac caccattatc aagcttgcgc
      601 cgaagagcat cattttcagc tttcgcatca gctaactcct tcgtgtattt agcatcgagt
      661 gcatcagcat cacgctggcg ctgctgcatg tcagtaatgg ttgcgttcgc cagcttcagt
      721 tctccggcgt ttttgtcgcg ctgctctttg taggtaatgg cgttatcacg gtaatgatta
      781 acagcccatg acaggcagac gatgatgcag ataaccagag cggagataat cgcggtgact
      841 ctgctcattg atctatcccc caacaggcta atgcgctttc ctggtcacga cgaataacct
      901 gtccatagca gttatttgaa cgtatgcggc aatcgcgccc aacatctttt atccaccagc
      961 gaatcgcctc gcatgcgccc ttacgatcac cggcattcag ccgcttataa aacgtcgacg
     1021 ggaaacactt accggggcca atgttatagg ggcaaaatga cgctataccc gctttctggg
     1081 gttcggtcag aggtacttta atattgcgct ccacccatgc cagcgcctta tcacgttcaa
     1141 tagcgttaac ctggtcacat ttttccttcg acagcttcat tcccggtatg acaggcttac
     1201 catccaccat tgtggcacca cgacagatgg tccatatgcc agaaccatca cggtatgccg
     1261 ttgtgtgatt accctctttt tcgttcagaa actggtcaag tatctgggga gcagacgcgc
     1321 ctgcggcaat cagcgccaga acagcagccg acaggccgta tttgattttt gcgctcatgg
     1381 atatttatca ggatttatcg gcaacaaata acgagccaac ttatatacgc cttttaagat
     1441 aagtcagccc cggctggatc cagtcatcag gctctttctt aaagggggag tattgaaatc
     1501 acgaagaaga gcctcccgca ccgcagcatc catgtctgca ccactggcaa gtgcttcaat
     1561 ctctgccgcc acctgcaaat accccatgca acggccaatg cgtttcataa ctctggtgtt
     1621 ttcgtctttc aggcatctga tagcgagatt aataattttc aggcagtgat gatgcgatgc
     1681 ccggacaggt tttccgttct cataaacgac gaactcaccc tcttcacagg aaaccctgaa
     1741 aggccgatgg ggttcctggt ctttatccgg tgcagacgca ataaaatcac ttttatgctc
     1801 ttcctctccg gcaccgttaa ccgtaatgac gtactcaggc cgcaggtaag ccgcctcttt
     1861 ttctgacaat gcggccggta aaaacttttc ctgcaaaaac ttcataaaag tctcgtgagc
     1921 gactttgtgg cagtaatcgt aaatcgccgc ataatgctca tcgcggcgtt tgttttcatc
     1981 ttcagaactc accaacgccg acaatttttt attaagctca gtgatttcat tttccaggtg
     2041 attgaatcgc tgattcattt cttcatgctt cattatttac tctccccgtg cagccttacg
     2101 cttgtcttct cttattttga aatacagatt cgtcagataa gtcagaagcc ccagaagcag
     2161 acttcccagc acaccaatcg cagcccactg tgatggcctg acctgatcca accactgcaa
     2221 aaaccagtag ccggcactgc cggcggaggt gccgtaggca atgcccgttg aaattttgtc
     2281 catggatttc atagcctcac ctccgcaaat aacggatggc gtagttttac actgagaaat
     2341 gaaagggatt tgaaaagaaa aaaacgcaaa agcgggcgaa acgatatata cagtaaggaa
     2401 agcactctat ccaacaaacc acccacagtt aatcggaata aaagcagagt gcttatgaat
     2461 gatcgcacgc ccgaaggtta gtatttctgc acagcaattt tgcaaaaaaa
//
LOCUS       DAEBXD010000255         2496 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEBXD010000255 DAEBXD010000000
VERSION     DAEBXD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.261"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..912
                     /locus_tag="KJL94_004243"
     CDS             <1..912
                     /locus_tag="KJL94_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD4412330.1"
                     /translation="ETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISE
                     FGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIE
                     GLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNA
                     QERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNKAQSFE
                     VVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYVDYKIN
                     LIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1076..1561
                     /locus_tag="KJL94_004244"
     CDS             1076..1561
                     /locus_tag="KJL94_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD4412331.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            1671..>2496
                     /locus_tag="KJL94_004245"
     CDS             1671..>2496
                     /locus_tag="KJL94_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD4412332.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYIN"
BASE COUNT          829 a          512 c          435 g          720 t
ORIGIN      
        1 gaaacgcaaa tcaatgatca aatgattggt tttggtcact gggaatatga ttttaaaggc
       61 tataacgatg aagccaacgg ctcgcgcggc aacaagaccc gtctggccta tgctggttta
      121 aaaattagtg aatttggctc tctggactat ggtcgtaact acggtgtcgg ctacgacatt
      181 ggttcatgga ccgatatgtt gccagaattt ggtggcgata cctggagtca gaaagatgtc
      241 ttcatgacat accgtaccac cggtctagca acctatcgca actacgattt ctttggctta
      301 attgaagggc tgaactttgc cgcgcaatat caaggcaaaa atgaacgtac tgacaacagt
      361 catctttatg gtgctgacta cacgcgtgcc aacggtgacg gtttcggtat ctcctcaact
      421 tatgtttatg atggctttgg tatcggtgcg gtatatacca aatccgatcg gacaaatgcg
      481 caggaaagag ccgcagctaa tcctctcaat gcctccggta agaatgcaga actatgggcc
      541 acaggtataa agtatgatgc caacaacatc tactttgcag ctaattacga tgaaacatta
      601 aacatgacca cctatgggga tggttatatc tctaacaaag cacaaagttt tgaagtggtg
      661 gcgcaatatc aattcgactt cggcttgcgc ccatcactcg cttacctgaa atcgaaaggc
      721 agagatctgg gccgctacgc cgaccaggac atgattgagt atatcgacgt tggtgcgacg
      781 tatttcttca acaaaaatat gtcgacctat gttgattata aaatcaacct gattgatgaa
      841 agcgacttta cccgtgccgt agatattcgc accgataaca tcgtcgctac gggcattacc
      901 tatcagttct aacgtcctct cctgaccagc ccttctctgg gctggttttt gatttaagtg
      961 ctgctgtatg actccttaag taaaaatgag caattcttga tatttctggt cattcacctc
     1021 aattaatatt attccatatc ctccggggac atcttactat aatatttatt gatgagtgat
     1081 tatgaccaat attaatacag cttgtgtaaa aaataatgcc agttatcaac ttaataacgc
     1141 attacctaac aaagaaacca tctccagcaa tttttgtgaa cgactagaac aatggggtaa
     1201 taaatcggtt aataatggtg aagaaagagc aattgccgtg gagcgaatta aagaagctta
     1261 caattcgaac atggcatctc ttgatttgtc atatcttgat ttgagtgaat taccgcctat
     1321 cccctcgaca gttaatacgt taaatctgga aaacaattgt ctaacttgtc ttgacttagc
     1381 tgacaatgcc agtctcgtta atatcaacct cagcgttaat aaaattaaca cgataacctt
     1441 tccaaaagaa ccaaaactgg aaaacattta tattgatcat aacaatctgg aaagtttaga
     1501 tttaaaaaat cagcattcat tggttaatct ggaagcgcaa aataatagcc taaaaaaatg
     1561 atatttctga tagttataaa ctgaaatttc ttaatcttga ttataacaag ctagcatcgc
     1621 tggatgtctc ccggcaagaa tctctgattg agttaagtgc ccatcacaat atgatcactg
     1681 accttatatt acacaatcat cccaggatga aaacaatcac tttaaacgac aaccatattg
     1741 cacatttaaa cgccaaaaac actacaaaac tggaatattt aaacttaagc aataacaatt
     1801 tactgccaac caatgacatt gatcaactaa tatcatcaaa acatctttgg catgtattag
     1861 ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca gtaagaaata
     1921 taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta accactcaac
     1981 caccagggct gcaaaacttc acctctatca atcttgataa taaccaactc acacattttg
     2041 atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatgct cttgagtcaa
     2101 taaattttcc tcaaggcaga aatgtaagta ttacacatat atctatgaat aataatgctc
     2161 tcagaaatat tgatatagat aggctttcat cagttactta ttttagtgcg gcacataatc
     2221 aactagagtt tgtgcaatta gaatcttgcg aatggctgca atacctgaat ctcagccata
     2281 atcaattaac tgatattgtt gcaggtaata aaaatgaact cttactgcta gatctatccc
     2341 ataataaact aacaagttta cacaatgtct tatttcccaa cttgaatacg ttacttatta
     2401 acaacaattt gctttctgaa attaaaatat tctatagcaa cttccgcaat gttcagacat
     2461 taaacgctgc taacaaccag ttgaaatata taaatc
//
LOCUS       DAEBXD010000256         2451 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEBXD010000256 DAEBXD010000000
VERSION     DAEBXD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.262"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..331)
                     /locus_tag="KJL94_004246"
     CDS             complement(<1..331)
                     /locus_tag="KJL94_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412333.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="KJL94_004247"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="KJL94_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBD4412334.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="KJL94_004248"
     CDS             complement(1692..2072)
                     /locus_tag="KJL94_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBD4412335.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..>2451)
                     /locus_tag="KJL94_004249"
     CDS             complement(2086..>2451)
                     /locus_tag="KJL94_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412336.1"
                     /translation="IPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFLVIVST
                     IALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGSMIFLI
                     CYFSTFSMLMLAPTDSSKQ"
BASE COUNT          671 a          554 c          561 g          665 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggtaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga t
//
LOCUS       DAEBXD010000257         2450 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEBXD010000257 DAEBXD010000000
VERSION     DAEBXD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.263"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            82..705
                     /locus_tag="KJL94_004250"
     CDS             82..705
                     /locus_tag="KJL94_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4412337.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(759..1931)
                     /locus_tag="KJL94_004251"
     CDS             complement(759..1931)
                     /locus_tag="KJL94_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD4412338.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2280..>2450)
                     /locus_tag="KJL94_004252"
     CDS             complement(2280..>2450)
                     /locus_tag="KJL94_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4412339.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNL
                     SQSRNKEGTNDV"
BASE COUNT          742 a          446 c          506 g          756 t
ORIGIN      
        1 tgtgaaggaa atggtactca gggggattcc agtacaggtg gtgcgcaatt agctataaat
       61 ggccttaacg gttcgtgcgc tttggtatta aacaacccat actttgaggc taatgccggg
      121 ggggcagacc ttgctataga taatactggg acgcgccctg taactgtggt tataaacggt
      181 ggaaattttc atcgcgtgag cagtgcaaga tacacgcaca cgaatattca agtaacaagc
      241 tcaggtgggg gaaaagttac agtcatactc aacggcacaa cgttccagag cgcaggggat
      301 tatcagccaa gtgcagaacg accatactgg ataacaggag ctaattgcga ggtagttgac
      361 attgggtgtg tttttacaga aacaaccagt aaggtaacat cggcttctgc attgtcagtc
      421 acacgctcgg gaaaaattaa cgccaatggt agtatcgacg ttgctactgg tgtttcatcg
      481 gttaacgtgg tttctacagg tgtgtatgat gttagctttt ctcatccgct cgcagcagtt
      541 gctagcggtt atattgtgca gataacacct atatctgcgc ctgattcagt tagttgtgat
      601 gttacttata ttggcgtaga tacatttagg gtgaccctgc gcaatacgct tagcggggca
      661 ggaatctcca gttcgtttgc atttagcatt accagactaa tataaataca ttcgccgccc
      721 catttggaca tatatgtagg gcggcttatg tttattgttt attgattgtt gtttgttaat
      781 gtgggtatta attcttttac taaagttgtg gtttgttttg ttaatttgat gaatggcgtc
      841 tctattaatt taaaagttag gcatgatgtg aacgtaataa gaaatgcagt tgtgcttacc
      901 agtatgataa atgtattttc agtgtaatta ttaggaataa tccacgtcat taggcaataa
      961 aggaatatcc catgcagaag gtaaacgctg taggtagttt ctcctagctt tctggttata
     1021 tttaatctta atattccata caaatcacaa cctgatgcga taatgataaa tgttattcca
     1081 caaagaatca gtggtagcgg tgcgtaagtt gttttgaaat acgtcatttc aaaaaccatt
     1141 atcgcagtta ttataattgg tgttactttt gctttggcaa ttccattgac tatctttgat
     1201 ttatttagca aaaatgccag gagcccaaaa agaaatgatg ctatgtgtat tttactgaaa
     1261 aataaaatga aaacaaatag cgatattgcg ctaatcataa ccatgtaaat actgactttt
     1321 ctttttatga gcgcggaaat taccggaaga gaaaaataaa agaaccattc atatacaagt
     1381 gtccatgtta cagcggcatt gatagtaaat gaatctttta cgtcattaat gttcggcata
     1441 cctagcgcag tgaatggcaa ccacttcatt attgacacaa aaagctcttc tgtggatacc
     1501 tgcattctcc atccagactt aaaacctaca atgataaaaa tgagacatag gctaactatg
     1561 aacattgggg ttaatcgtag taatcttgat acataaagcc ttgtccagtc ctgatctcca
     1621 gagataatct ttgaaaagaa cagataacca gtaatcataa aaaagaatga cacaccaact
     1681 tggcctaaat ttgctaacag atttgatgaa ggtgcttccc atactccaga tgacaagtat
     1741 ccattccaaa ttgctgcgtg atgaataagc acgaaaattg ctaagaaccc ccgcattcca
     1801 tctatttgat tattcctgcc gtgctcaaca agatttattg gggaaatttt atcaaataac
     1861 cccacagcta ataacgacat caatatcacg gatgttatta ataataaact gttaatttca
     1921 atcatatgca tgctctatta cgcaattaaa atttctccaa gtatactgac ttttagttaa
     1981 tgatcatata gttatgtgta actgatgaaa caaaacggag acacaccaag ctttgcactg
     2041 gattgcaagg ctttgtgctc ttctgtgaat gtgtggctat atgtttgaag atcgttgtgc
     2101 cgtatttgtg acatatatat ggcaacatca tgcatcaact ttctgtttgt gtcatcaact
     2161 atagcttagt gaatgcggtt aatgcttgct aaaacagata gttatgattg gtgctacaga
     2221 ttcgtaatgc gaaggtcgta ggttcgactg ctattatcgg caccatttaa atcaataagt
     2281 tacacatcat tagtaccttc cttatttctt gactgggaca aatttgggac cgatgggttc
     2341 agaatcaagt ctatttgccg tgcgtgttcg gtaaggtgat taggtgcgag gtgagcatat
     2401 cgacgaacca tttcgataga ctcccagcct cccatttcct gtaacactga
//
LOCUS       DAEBXD010000258         2432 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEBXD010000258 DAEBXD010000000
VERSION     DAEBXD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.264"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            65..1543
                     /locus_tag="KJL94_004253"
                     /pseudo
     CDS             65..1543
                     /locus_tag="KJL94_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2432
                     /locus_tag="KJL94_004254"
     CDS             1540..>2432
                     /locus_tag="KJL94_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD4412340.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          629 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa ca
//
LOCUS       DAEBXD010000259         2392 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEBXD010000259 DAEBXD010000000
VERSION     DAEBXD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.265"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="KJL94_004255"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="KJL94_004255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBD4412341.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2392
                     /locus_tag="KJL94_004256"
     CDS             1647..>2392
                     /locus_tag="KJL94_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4412342.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGG"
BASE COUNT          639 a          524 c          621 g          608 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc tt
//
LOCUS       DAEBXD010000260         2390 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEBXD010000260 DAEBXD010000000
VERSION     DAEBXD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.266"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..107
                     /locus_tag="KJL94_004257"
     CDS             <1..107
                     /locus_tag="KJL94_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBD4412343.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="KJL94_004258"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="KJL94_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBD4412344.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="KJL94_004259"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="KJL94_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBD4412345.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2390
                     /locus_tag="KJL94_004260"
     CDS             2210..>2390
                     /locus_tag="KJL94_004260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBD4412346.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          653 c          673 g          537 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
//
LOCUS       DAEBXD010000261         2336 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEBXD010000261 DAEBXD010000000
VERSION     DAEBXD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.267"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..402
                     /locus_tag="KJL94_004261"
     CDS             <1..402
                     /locus_tag="KJL94_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4412347.1"
                     /translation="SRAVIGWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(506..973)
                     /locus_tag="KJL94_004262"
     CDS             complement(506..973)
                     /locus_tag="KJL94_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD4412348.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(1039..1278)
                     /locus_tag="KJL94_004263"
     CDS             complement(1039..1278)
                     /locus_tag="KJL94_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBD4412349.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1413..2018)
                     /locus_tag="KJL94_004264"
     CDS             complement(1413..2018)
                     /locus_tag="KJL94_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4412350.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(2133..>2336)
                     /locus_tag="KJL94_004265"
     CDS             complement(2133..>2336)
                     /locus_tag="KJL94_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4412351.1"
                     /translation="DGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSL
                     SFSKLVELHDKVIGHYLNIKHYQ"
BASE COUNT          699 a          527 c          467 g          643 t
ORIGIN      
        1 tcacgtgccg ttattggctg gtcaatgtcg ccacgcatga cggcgcaact gccctgcgat
       61 gccctgcaga tggcgctgtg gcggcgtaag aggccccgga acgttatcgt tcacacggac
      121 cgtggaggcc agtactgttc agcagattat caggcgcaac tgaaacggca taatctgcgt
      181 ggaagtatga gcgcaaaagg ttgctgctac gataatgcct gcgtggaaag cttctttcat
      241 tcgctgaaag tggaatgtat ccatggagaa cactttatca gccgggaaat aatgcgggca
      301 acggtgttta attatatcga atgtgattac aatcggtggc ggcggcacag ttggtgtggc
      361 ggcctcagtc cggaacaatt tgaaaaccag aaccttgctt aggcctgtgt ccatattacg
      421 tgggtaggat cacaagacct actgctatca caattactga gagaaaaaaa agcatccgat
      481 gattcattta tgctgtccta aaaaattaac aaaacaatgg gaaaacagga tatgtaagca
      541 gaaaagccaa tgaaattggt acagcataag gcacaccgtt ttcacgaatt gattttcgaa
      601 aaaaaagaag acctgccaga acaaccagag cgccgcaaaa ggtcattgcg acgagaaaat
      661 tagccagttc atgttcatca atagctaacg aaagaacagt taacagcttg acatcaccgc
      721 cgccaattat ccctgcggaa aaaagaaaga agcccacgag caaaacgctc ccggctgcaa
      781 ccagattggt tttttgatgc tgtaaaatga gtgccaaaaa aataagcacg gttaacacca
      841 gtaaataacg atggctaata attctgagta atatatcccc ccatgaaata aaaataagaa
      901 agagcgcaac aaataaaaga tcgactctta gtaaccatat tgctgccatt gaaagactcc
      961 atgtaccata caaggttgct gccagacata ttcctttacg tctgacagcc tctagagagt
     1021 ttttttattt tttataagtt acggattact cgttccggaa atcgttacct gttgaattgc
     1081 tgcagcgata gcgtcgaatg catctttaat tgcgcccagg aagcctgagt tctgatcgcc
     1141 aaagatgaaa gcaagcagag ttgccatagc aacaccaatc aatgcatatt cgattgcagt
     1201 aacaccacgt tcatccttcg cgaaacgcgc aaagcactgt ttagttttac catagtactt
     1261 agtaataaaa gtattcatgt tttcctcgta tattattatt taaattcaaa ccaccagctt
     1321 catctgcgtg tcaccggtat cccatatttt ttggatgtgg ctataattat ccattcccgc
     1381 tatttagtct tcaaaaaaaa ttacatttaa tatcatttat cttgattaag aaatagtaag
     1441 actaaaaaaa caagcgtcgt cagccagcac actgctaaca cggtaagaac gctaataaaa
     1501 acactccact gcttcgttct ataccattca atatttttgt cagtatgttt tattgggagc
     1561 agctcttcag tagagaattc ctggctgtta tcattctcag gaatgtttgc atcagtgttt
     1621 acttccctgg cctcgctatc gttggtatag ctactactgt cttcatcact gtcacgctca
     1681 ttctcacaga catgtttctc gacctttccc agatatctga cgccttttcg cggaatggtt
     1741 ttaattagag aactcgacaa acctgcgacg tctaatgcct ttctaagcat gtaaatctgc
     1801 ccataataac tgctgtcgct taccgaacct ttctgctccc gccaaacctc attgataata
     1861 tgttgcttat cggattctcc ctgtagtaaa aggagtaaaa atcgatagcc gttttcagac
     1921 agattgatta cattgcctgt actggtggtg atacgtcgag acgctgggta aaacttaact
     1981 ccctgcccta aagaaaaata aaaacggtca tcgctcataa gtaccgaccc ctgttttctt
     2041 cttcacatga ctactcatta caactttgta tgaagtaaga tgcaaaatca tattgtttac
     2101 attaagttaa gcaattaggt aatgactcca acttactgat agtgttttat gttcagataa
     2161 tgcccgatga ccttgtcatg cagctccacc aattttgaga acgacagtga cttccgtccc
     2221 agccttgcca gatgttgtct cagattcaga ttatgtcgct caatgcgctg agtgtaacgc
     2281 ttgctgataa cgtgcagctt tcccttcagg cgtgattcat acagcggaca gccatc
//
LOCUS       DAEBXD010000262         2318 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEBXD010000262 DAEBXD010000000
VERSION     DAEBXD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.268"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..371
                     /locus_tag="KJL94_004266"
     CDS             <1..371
                     /locus_tag="KJL94_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412352.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            371..718
                     /gene="tnpB"
                     /locus_tag="KJL94_004267"
     CDS             371..718
                     /gene="tnpB"
                     /locus_tag="KJL94_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD4412353.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            738..1967
                     /locus_tag="KJL94_004268"
                     /pseudo
     CDS             738..1967
                     /locus_tag="KJL94_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1971..2130
                     /locus_tag="KJL94_004269"
                     /pseudo
     CDS             1971..2130
                     /locus_tag="KJL94_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          564 a          609 c          637 g          508 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctt tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatgcga aatggcttta acggcctggc ctcaaaagtt cagaacgtcc tgaaggatga
      481 cccgttctcc gggcatctgt tcatcttccg cggacgccgg ggtgaccaga taaaagtgtt
      541 gtgggctgac agtgacggac tgtgcctctt taccagacgc ctggagcggg gccgcttcgt
      601 ctggccggtc acccgtgacg gcaaggtgca ccttactccg gcccagttat ccatgcttct
      661 tgagggcatc gactggaaac acccgaaacg aacggaacgc gctggcatcc gcatataacc
      721 cgttgtaaag tgaggatatg gacacctcac ttgctcatga gaatgcccgc ctgcgggcac
      781 tgttgcagac gcaacaggac accatccgcc agatggcgaa atacaaccgc ctgctctcac
      841 agcgggtggc ggcttatgct tccgaaatca accggctgaa ggcgctggtt gtgaaactgc
      901 agcgcatgca gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagatac
      961 tggaagcaca ggagcgaatc agcgcacttc aggaagaaat ggcggaaacg ctgggtgagc
     1021 aatatgaccc ggtactgcca tctcccctgc gccagtcttc agcccgtaaa ccgttacctg
     1081 cctcacttcc ccgtgaaacc cgggtcatcc gtccggaaga ggaatgctgc ccggcctgtg
     1141 gtggcgaact cagttctctg ggatgtgatg tgtcagagca gctggagctt atcagcagcg
     1201 cctttaaggt tatcgaaaca caacgtccga aactggcctg ttgccggtgt gaccatatcg
     1261 tgcaggcacc ggtgccttca aaacccattg cacgcagtta tgccggagcg gggcttctgg
     1321 cccatgttgt tgctggaaaa tatgcagact atctgccgtt ataccgccag tcagaaatat
     1381 accgtcgtca gggcgtggag ctgagccgtg ccacactggg gcgctggaca ggtgctgttg
     1441 ctgaactgct ggaaccgctg tatgacatcc tgcgccagta tgtgctgatg cccggtaaag
     1501 tccatgccga tgatatcccc gttccggtcc aggagccggg cagcggtaaa acaaggactg
     1561 cccggctgtg ggtctacgtt cgtgatgacc gtaacgccgg ttcggaaatg cctccggcgg
     1621 tctggttcgc gtactcaccg gaccggaaag gtatccatcc acagaatcat ctggccggtt
     1681 acagcggagt gcttcaggcc gatgcttacg gtggttaccg ggcgttgtac gaatccggca
     1741 gaataacgga agccgcatgt atggctcatg tccggagaaa aatccacgat gtgcatgcaa
     1801 gagtgccaac cgacatcacc acggaagccc tgcagcgtat cggtgaactg tatgccatcg
     1861 aggcagaagt ccggggatgt tcagcagaac agcgtctggc ggcaagaaaa gccagagccg
     1921 cgccactgat gcagtcactg tatgactgga tacagcaaca gatgaaaatt cattcgctga
     1981 aaatggaatg tctccacgga gaacactatt atccatcggg aaatagtgcg ggcaacagtg
     2041 tttagttata tcgagtgcga ttacaatcgc tggcgacggc accgttggtg tggtggtctc
     2101 agtccggaac aatttgaaaa ccagagcctc acttacgact gtgtccatat tatgtgggta
     2161 ggatcaataa agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg
     2221 tatcataaac atgcttgaca acttaattag ggccccctct tccttttatg gtgtaattgt
     2281 ttgattgaat acttccgggg atttcagtat gaaatgaa
//
LOCUS       DAEBXD010000263         2279 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEBXD010000263 DAEBXD010000000
VERSION     DAEBXD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.269"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            441..536
                     /locus_tag="KJL94_004270"
                     /pseudo
     CDS             441..536
                     /locus_tag="KJL94_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            534..664
                     /locus_tag="KJL94_004271"
                     /pseudo
     CDS             534..664
                     /locus_tag="KJL94_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            773..1039
                     /gene="ospE1"
                     /locus_tag="KJL94_004272"
     CDS             773..1039
                     /gene="ospE1"
                     /locus_tag="KJL94_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBD4412354.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1431..>2279
                     /gene="ipaH1.4"
                     /locus_tag="KJL94_004273"
     CDS             1431..>2279
                     /gene="ipaH1.4"
                     /locus_tag="KJL94_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HBD4412355.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNQ
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          752 a          488 c          390 g          649 t
ORIGIN      
        1 tctcgtctgg ggaatatctc cccggcagcc ttcaggataa aatattatca gatgactgct
       61 tagaaaaaga acaaatggtg gtgtccgcta ttgccagtac acctcaagct tcttaccata
      121 tttgaaataa agctcaatag cgcgaatttt ttgttcttca gtaaacatgt ttcatcttct
      181 gagagtccag gaaatcgtcc gcatccccat taccgtctta tatcactggc gctgacatgt
      241 gctcattgat gaagtgaatc tgaatgacat gttttgccat atcaacgcca accggtgtat
      301 atttcatttg tggagtctcc agtctgagga cgctttatgc atcccgtatt gggcagtatg
      361 atgccggaaa gctgcgaggc tccacacctg ccagtttcac ccttttaacc atcatactac
      421 tccgtgttta attgtgtggg atgcgttcat tacattttaa cagtgtcacc ccgtcggcca
      481 cgaaagataa acaggtgacc agaaaaggga tcttcatcca gcacatgcta tatcttgaat
      541 gtaacaacct gaccctccgc acccgcatca agcggctggc acataaaaca atctgcttct
      601 cgcgttccgt ggaaatccac gaaaagtcat cggctccttc attgaaaaac acatattcta
      661 ctgattggaa gcgccacccg aaaaagtatc gtttttttac agtaaacttc atttagccga
      721 ctataatgta aaaaaataaa ttttttgcca aaatataaca taaggaagta atatgcttac
      781 acaaactata ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa
      841 tcctgtatta ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc
      901 agctatatac gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc
      961 tacagaaatg caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca
     1021 acggtattct gatttctgaa gttaatttca gactcgttta agctccattt tcaaaaaaaa
     1081 tatatcaggg tttgttacta ataacttctg aaaacagaaa aaaaaataga gcagtaattc
     1141 tatttcagag aggagtaact ccatctatcc tcgatacgta ctgaacgtta tcgtgatata
     1201 ttcatcatgc tttcttcacc attattcgag tatagggaga gacgcaatga ccttaaatac
     1261 ctttttatgg ctgaattacc attctcagga ataacagcag actccttccc atagactgct
     1321 tagggggaaa gcaatacccg gagaaagggt acaaagcaaa ctgttttaaa attaataagc
     1381 tatattctat ttaactagaa ttcttatcgt tgataagaat tctagttata atgattaaat
     1441 caaccaatat acaggcaatc ggttctggca ttatgcatca aataaacaat gtatactcgt
     1501 taactccatt atctttacct atggaactga ctccatcttg taatgaattt tatttaaaaa
     1561 cctggagcga atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata
     1621 ggctgaaaat atgtttacaa aatcaagagg cagaattaaa tttatctgag ctagatttaa
     1681 aaacattacc agatttaccg cctcagataa caacactgga aataagaaaa aaccaattaa
     1741 cacatctccc tgatttacca ccaatgctta aggtaataca tgctcaattt aatcaactgg
     1801 aaagcttacc tgccttaccc gagacgttag aagagcttaa tgcgggtgat aacaagataa
     1861 aagaattacc atttcttcct gaaaatctaa ctcatttacg ggttcataat aaccgattgc
     1921 atattctgcc actattgcca ccggaactaa aattactggt agtttctgga aacagattag
     1981 acagcattcc cccctttcca gataagcttg aagggctggc tctggctaat aattttatag
     2041 aacaactacc ggaattacct tttagtatga acagggctgt gctaatgaat aataatctga
     2101 caacacttcc ggaaagtgtc ctgagattag ctcagaatgc cttcgtaaat gttgcaggta
     2161 atccattgtc tggccatacc atgcgtacac tacaacaaat aaccaccgga ccagattatt
     2221 ctggtcctcg aatatttttc tctatgggaa attctgccac aatttccgct ccagaacac
//
LOCUS       DAEBXD010000264         2264 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEBXD010000264 DAEBXD010000000
VERSION     DAEBXD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.270"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            140..901
                     /locus_tag="KJL94_004274"
     CDS             140..901
                     /locus_tag="KJL94_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD4412356.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            945..2024
                     /locus_tag="KJL94_004275"
     CDS             945..2024
                     /locus_tag="KJL94_004275"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4412357.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            2021..>2264
                     /locus_tag="KJL94_004276"
     CDS             2021..>2264
                     /locus_tag="KJL94_004276"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD4412358.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT          663 a          402 c          544 g          655 t
ORIGIN      
        1 aaattaagaa aagccatatt tacgatatgg gcaacataaa tataatctaa aaaagagtaa
       61 ggcgttgtag gttggttccg cgttcattac cttgttagtg aacgcggatt tatcagttgt
      121 cgaaatggtt tttataataa tgaaaaattt taatgtacta accaaagtcg tattttgctt
      181 gtcgttttta tcaagcgcat ttcccaccta tgctgcggat gatgtcaaag tgactcaaaa
      241 cacttcagtt tttaaactga agcttagtgc aacgcgatta atctatgaac aagggtcgga
      301 tggcgcagcg ataactgtga caaatccgca ggattggccg atgcttgtgc agagtaaagt
      361 ctttgaagaa gataaagtaa ctgcagcgcc ctttattgtc actccgccac tgttcaggct
      421 taatccaggc cagcaaaata aaatgtgggt tgtgcagact gcgccagctt tacaagacag
      481 ggaaaaatta cactggctat gcgccaccgg cattacgcct aaaaagagtg atgtttgggc
      541 cgagaaagat aaagaaaaaa ttgctgaaaa tgtgaacctg aacatccagg ttaacgtaca
      601 tacctgcata aaacttatta ccaggccatc tggtattgaa ggcaagatgg ttaatgcgag
      661 tgagaagtta tcgtggaaaa agcagggtga taaaattgtc gcggtcaatc agtcgccgtt
      721 ctacatcaac ctttcttctt tacaggttgg cggtagcaag attgagaaac tagattatat
      781 tgcgccgttt tcgcaacatg agtttgttat gccaaaaact agcggccagg atattaaatg
      841 gtctgtgatt actgatgaag gtggtgagag tcgtttgttt atatctcatt tgcaatcata
      901 atgtattaca tttttattga atatttttat gccatgcata ttccatgttt tatcgactgt
      961 cattaatttt gataatggcc ttacttgcgg gtcagttaag tgcccaggag tggtcatttg
     1021 attcttcaca gttggaagga aacgttagtg cggacactgt ggcaatgttt aatcagggag
     1081 agcaactccc cggaaattat cgtgtggaaa tttatcttaa cggtgaaaaa gttgatgttg
     1141 gggagtttcc ctttcatcgt ccggaatccc ctgaagaaaa agagcttgtt ccatgtttaa
     1201 ccgttgatga ccttatccac tatggcatta aaattgataa aagcagcagt gatactgaca
     1261 ataaaaagaa tcagtgtttt aaatggaact ctattgaagg tttgaaagta aattatgatt
     1321 ttgatagcca gcgcgtacag ataaccgttc cacaactgta tctgcaagat aaaaaaagta
     1381 gtttagcacc agtgtcgtta tggaatgagg gtgtagctgc attccgcatg gtgtatcaga
     1441 caaatattga tatttccaaa caaaatgata atcaatctac cacgcgtaat tctcgttatg
     1501 gcaggttcac tccagggttt aatcttggtg cctggaggtt tcgtagttca gtgacatgga
     1561 gcaaggaact tggccagtca gaaagatggc aaaggggtta catgtggttt gagcgaggca
     1621 taaatgcgat taaaagccga ttaacgcttg gagaatctta tacgtcgtct gaggtgtttg
     1681 acagcatacc ttttcgcggc gggatgttag cgaccgacga tgcgatgact ccgccggagg
     1741 acagctacta cacacctgtg gtgcatggaa tagcacaaag tgaggcgcag gttattatta
     1801 aacaaaatgg gcaaattatt tttacccgtt ctgtgccgcc agggcctttt gcgctggata
     1861 atttaccaac gctggccgtt ggcggggagc ttgatgttac agtgagagag agtaatggcg
     1921 aggagcagta tttttccgtg ccatttcaaa cgccggccat cgctctgcat gaaggatatt
     1981 tcaaatatag tgtgatgggg gggaatataa agaagaaggt ataaaaagct cccctgtctc
     2041 cgtgggcgaa atcacggcga tgtacgggct tccttatggc gtcacggtgt atggagggat
     2101 tcaaaacgcc actcatttta atgcaatatc tacgggtatt ggcatttctc tggggctgtt
     2161 agggagttta tcgacagata ttacgcgttc gattgcgaat ttatattatg ggaataaata
     2221 tcgtattcgt tatagtaaat ccattagtga ttttggtact caat
//
LOCUS       DAEBXD010000265         2217 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEBXD010000265 DAEBXD010000000
VERSION     DAEBXD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.271"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..1224
                     /locus_tag="KJL94_004277"
     CDS             <1..1224
                     /locus_tag="KJL94_004277"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBD4412359.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="KJL94_004278"
     CDS             1224..1898
                     /locus_tag="KJL94_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4412360.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
BASE COUNT          561 a          455 c          562 g          639 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgatttt
//
LOCUS       DAEBXD010000266         2190 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEBXD010000266 DAEBXD010000000
VERSION     DAEBXD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.272"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..701
                     /locus_tag="KJL94_004279"
     CDS             <1..701
                     /locus_tag="KJL94_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412361.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            703..1308
                     /locus_tag="KJL94_004280"
     CDS             703..1308
                     /locus_tag="KJL94_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBD4412362.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1295..>2190
                     /locus_tag="KJL94_004281"
     CDS             1295..>2190
                     /locus_tag="KJL94_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4412363.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          533 a          508 c          611 g          538 t
ORIGIN      
        1 acgcccgtgc cggtgtgccg ggcagcgatg aggaaacccg tgcggacttt gaaaaccgcc
       61 gacgtaattc agttgcccgt aatgcccgga atattctgga agccatccgg ggtgaaatac
      121 tctccacggt agaaaacgtg gtggatgttt acgtcaccca taatccgaaa aaaacagaac
      181 aaaaagccgg agtcagtcag tatccattaa cacccggttc gctttatgtt ggcgtgtacg
      241 gcggcagccc ggcagatatc gcggcggcca tctggcgtaa ggctccgccg ggtattgata
      301 tgaatggcaa cacaacgttc actgttgcag ataaggagta cgatccgccg tatcctgaat
      361 acgtgatcag ctggcagaca ctcaaacccg tcagtctgca tgtcagtgtg acgctgaaaa
      421 aaagtgacta cctgccttca gatattaccc gacaggtaca gcaatccgtg ctggacgcat
      481 ttaatggtac agatggtggt ctgcgggcaa gagttgcctc tgttgtctct gccgggcgct
      541 actatgccgg catttacaaa accgatccgg aacatattga tattctgggc cttactgtga
      601 gccgtgatgg ttcgtcatgg acaacggctg tcactttcgg gatagatgag attccggttc
      661 tggatgtgtc ggatatcagt gtgaaactcc aggaggcata acgtgcagaa tgtggctgcc
      721 actgtgcttg cgcagtatgc tgccagcccc cgactcaatg ccctcattaa cagctttaac
      781 gcagcgcttt cccccgacag ttttatcagt gatttttatg gtcttatctg gaacatcgat
      841 accgcagaaa agtatggtct tgatgtctgg ggaaagattg tgggtgtcag ttgctggctg
      901 acggtaaagg acgattttaa ttacctgggc ttcagcgagt ccaggatgga caccccggta
      961 atggatgatc cctgtccgtt taatcaggca ccgttttaca acggaaaatc ggatacccgg
     1021 actgttgacc tgtctgatgc tgtataccgg cggctgatac tgatgaaagc catgtcgaac
     1081 attactgact gttccgttcc ggatattaac ctgatgctga gatttatgtt cggaaaaaaa
     1141 cgccgggctt atgttctgaa taatggcggg ctgaggatga gttacgtctt tgagtccgcg
     1201 ctctcgttgg cagaactggc gattatccag tcgtcgggtg cactgccatc cccgccgggt
     1261 gtttatgttt cagtagtttt aaaggagtcc cgtaatgaag gccagtgata aaccccgcca
     1321 gctggcggtc ccctttgcga gtaccggaga taaaaaccgt atcccggaca aggcgacaca
     1381 gcagaccaga gagagcggta atgctgcgta tgattcaggt tttcctccgg tgaccatgac
     1441 agcggtctca gcgggaggta taccgccaca cggcaaggat tttaacggtc tgatgtacga
     1501 tattaccgca gcaatacggt tcgcccaggc tggcggtttg tacacgtata atgccggttt
     1561 tgcgggggcc attggtggat atgcaaaagg agccattctc gccggagtcg caacaacagc
     1621 ggtctggctg aataccacgg acgataacct gaccgatcct gaaggctccg acagtgcggg
     1681 ctgggtaaat cttcttgagg atccgaaaag gatattcctg cggcagaaga acaatctgtc
     1741 agaccttcag aataaaggga cggcacggga taatcttcag gtttacagta aagagcagtc
     1801 agatcaacgc tatgttcatc gggaaggcga taaaataacc ggagagctga aaatccgtgg
     1861 tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg atttttcgtc gttcggaaga
     1921 gtgcctgcac cttattccca ccagtgaagg tcagggggaa aatggcgata ttggtcccct
     1981 gcgcccgttc accattaatt tgcggacggg tgaaatatcc atgtcgcata aagtgtctgt
     2041 tggcggtggt tcgcaggtca atggtgcgct gggtatcggc gttcagaacg ccctgggggg
     2101 gaattcaatt gttcttggtg ataatgacac cggatttaaa cagaatggag acggtattct
     2161 ggatgtttat gctaatagtc agcgtgtatt
//
LOCUS       DAEBXD010000267         2187 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEBXD010000267 DAEBXD010000000
VERSION     DAEBXD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.273"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(220..384)
                     /locus_tag="KJL94_004282"
     CDS             complement(220..384)
                     /locus_tag="KJL94_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412364.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="KJL94_004283"
     CDS             1286..1570
                     /locus_tag="KJL94_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD4412365.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="KJL94_004284"
     CDS             1558..2043
                     /locus_tag="KJL94_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4412366.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAEBXD010000268         2132 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEBXD010000268 DAEBXD010000000
VERSION     DAEBXD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.274"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..866)
                     /locus_tag="KJL94_004285"
     CDS             complement(<1..866)
                     /locus_tag="KJL94_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBD4412367.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTFGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDA"
     gene            complement(887..1258)
                     /locus_tag="KJL94_004286"
     CDS             complement(887..1258)
                     /locus_tag="KJL94_004286"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBD4412368.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALAST"
     gene            1340..1567
                     /gene="vapB"
                     /locus_tag="KJL94_004287"
     CDS             1340..1567
                     /gene="vapB"
                     /locus_tag="KJL94_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBD4412369.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1567..1965
                     /gene="vapC"
                     /locus_tag="KJL94_004288"
     CDS             1567..1965
                     /gene="vapC"
                     /locus_tag="KJL94_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBD4412370.1"
                     /translation="MLKFILDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1974..>2132)
                     /locus_tag="KJL94_004289"
     CDS             complement(1974..>2132)
                     /locus_tag="KJL94_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412371.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
BASE COUNT          472 a          633 c          528 g          499 t
ORIGIN      
        1 gcatcgctgt aaccggccgt ccgggggaca cttttctccg gatgtacggt cacccggttc
       61 tgtttcatga tgtcacgact gagtgcccgg actgacagct catcgagcat atgaatcccg
      121 aacgtgaaca gccccttctc acgatcgagg gggataagct gctcacggct gatggcctca
      181 tcgataccgg cgcgtgcccg ttcaatcaca ccattttccg gcggcagtat gccgaccgtc
      241 ctggccagca ggtccgtata cataaactgc actttgcgtt cacttaatcc ggcaatggcc
      301 tgtgtcaccg cctgctgcac atccggcccg tcctgtgaag cgggtccggg tgtctgcgtg
      361 cgggtatatg cccgttgttc cgctgcgtca cgatatgccc ggatgtcgaa cccggtttcc
      421 ttcagcgtct gcatccactc ggccattctg acttcaggat cgacgtgctg tttggattta
      481 cgcgtatcca gcgccgccac atcccgggat ttcagcgagg cgtcttcccc gacggcctcc
      541 cggatagtct gtgagcgtcc ggaaaaggcc tccaccggta cacccggcat ttcccacata
      601 ccgtgcttac cgaccacttc agtttcatag cccagcgcct caacctgctc tttcagtttt
      661 tcccggtaga gcctgccaaa ggcaatctga ttagcgtaca cattctcaat gaaccccgtt
      721 ttccccactt tgtcactgct cagtgtcttc cattcgccgt tatactgcgt aacattagtc
      781 accaccgcat gcgtgtgtaa ctgtggttcc tgatcgcgac tggtgtcgtg gttaaacagt
      841 gccatcacca gattaccggt cagcaccgtt tctgactgtc cgtccgtcag gtggaggcca
      901 gtgcctccac ctgacggaca gcaaaatcca cggcctggtt atgtgcttca ataagacgtt
      961 tgtccccccc gagcatggcc atcatggaga tacttttggg agcggagaag gtcaggtcgt
     1021 agccgggacg atgcctgtta ctgccatcct gcatgcggct taaatccgcc ccgtccggca
     1081 gcctgccctc cagaaggcgg gtaaagacat ccttatcgac actgccctgc agccccagtt
     1141 gttcagcccc ctggccggcc cagcgttctc ccatgctgcc cagcacatag taattatcct
     1201 tgtcggtata ataattaccg gcacttccgg ccgatctgac ctgcgcgata ctcatcataa
     1261 acgtatatcc ctttgacata tcccggtatc aatcccacaa tagatataca caagacatat
     1321 ccacataagg aggcaaataa tggaaaccac cgtatttctc agcaaccgca gccaggcggt
     1381 cagactgcca aaagcggttg cattgccgga aaacgtaaaa cgcgttgaag tgattgctgt
     1441 cggacgaacc agaatcatta cgccagccgg agagacatgg gacgaatggt tcgacggaca
     1501 cagcgtcagc accgatttta tggataacag ggaacagccc ggcatgcagg aaagggagtc
     1561 attctgatgc tgaagtttat tctcgatacc aacatctgca tttttacgat aaagaacaaa
     1621 cccgccagcg tcagggagcg ttttaacctg aaccagggga aaatgtgtat cagttcggtc
     1681 accctgatgg agctgatata tggtgcagaa aaaagccaga tgcctgaacg taatctcgct
     1741 gtgatcgagg gatttgtttc ccgcattgat gttctggatt acgacgctgc tgcagccaca
     1801 cacaccggcc agataagagc agaacttgcc cgtcagggac gccctgtcgg gccatttgat
     1861 caaatgatcg caggtcatgc ccgcagtcgg gggctgatta ttgtgactaa taacacccgg
     1921 gaatttgaac gtgtgggcgg cctgagaact gaagactgga gctgacctgt tcatcagaaa
     1981 tcatctcccg gctcaacatc ctccccgcgt tcccggtgca cattgatgtt cacctcttca
     2041 cgacgctgca tctgctgcca ggtgtccgga tgattttcct gctgccatgc ctcataagcg
     2101 gccatatcca ccacttcacc ggattcactg at
//
LOCUS       DAEBXD010000269         2132 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEBXD010000269 DAEBXD010000000
VERSION     DAEBXD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.275"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..1073
                     /locus_tag="KJL94_004290"
     CDS             <1..1073
                     /locus_tag="KJL94_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBD4412372.1"
                     /translation="TDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNP
                     LSQSLYTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVL
                     TVEQQAETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSIT
                     ALEGEIRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGF
                     AVVADEVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAAT
                     ALDGIGQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSA
                     KSVAQLTSALSELAKQFWEKRG"
     gene            complement(1366..1827)
                     /locus_tag="KJL94_004291"
     CDS             complement(1366..1827)
                     /locus_tag="KJL94_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD4412373.1"
                     /translation="MMKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSQKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNSDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT          601 a          512 c          487 g          532 t
ORIGIN      
        1 ttactgatac atcaataagc agcttattgt tagccacttt aatgtcagga agcttaagct
       61 ctgttagtgt tttggctatt ctctctcctc ttggaagact ggttgaaaga gccaggaata
      121 tttccaataa cccattaagt caatccctct acactgggcg caccgatgag tttggccaaa
      181 tagagtttgc tttacgaatg atgcaagctg aaacaggcgc catagtaggt cgcataggtg
      241 atgcatcaaa tcggcttagc gaacacaccc gaggcctact aaaggatatt gagtcaagca
      301 atgtacttac agttgagcag caggcagaga cagatcaaat agcaacggca gtaaaccaaa
      361 tggtggcaag cattcaagag gttgcgagca atgcacagca tgctgcagat gcggccggaa
      421 gagcagacac tgagacggca tctggccagc gtctggtagc ccacacaagc cagtcaatca
      481 ctgcccttga aggtgaaatt aggcaagcca ctcaggttat tcatgagctt gaaggtcaaa
      541 gtaacgagat atcaaaagtt cttgacgtta tacgagggat cgccgagcaa acgaatttgt
      601 tggcactcaa cgcagcaatt gaggccgcgc gtgctggtga gcaggggcgt ggttttgctg
      661 ttgtcgccga tgaggttcgc agtcttgctg ctcgcacaca gcaatcgaca acggatattc
      721 aaagcatgat cagcgctcta caagagcgag cgcaatccgc tgttacagtc atggagcaaa
      781 gtagtcggca agcgcacacg agtgtagctc acgcagagga agcagctaca gctcttgatg
      841 gaattggcca acgcgttaac gaaattaccg acatgaacgc gcaaatagcg actgcggtcg
      901 agcagcaggg agcagtaagt gaagacataa accgcagtat tatcaatata cgcgatgctg
      961 ctgataccaa tgtacagacc gggcagaata atttgcaaag tgcgaaatct gtcgctcagt
     1021 taactagcgc tctgagcgaa ctggcaaaac agttttggga aaaacgagga taacgctttt
     1081 cagtatctcg acagggataa cttactttac catcggttat cccttttagc cgcacaaatt
     1141 ttagccatgg cttttcagga agtcagggct atcagaatgg ccttagaaag cctagtcaaa
     1201 gagctgtcac gagaacaccg ttagcttagc gtacgatttt ttccgaattc tgcggttccc
     1261 cctattgact gatgaacatc gccgtaactg atacagccat tcctgttttg tttttgccat
     1321 ttttattccc gctgagaaca aatgaatgat aggattgcca tcgctttatt tacggtgcct
     1381 ccatatccag gggggaaccg gatcagcatc tgaccagagc ccacgttttt gttgtctggc
     1441 ttcgttctga aggacaggca gcacgggatc tgagttgtac tgctcatata cccaggcagc
     1501 cccggcccgg accataaact gattaatatt cattccgtct ggtgcataaa cctgcccgag
     1561 aatacgacca taacgatctc gctgaaaata tgtgacggta accgtttttc ctgcgaccag
     1621 tgatttcatc atgtctgttg accaccggcc ataatcctgt ttcttttccg gtgcatcaat
     1681 atttaccaac ctgatccgca ccgccttctg tgagtccatg acctcgatgg tatcaccgtc
     1741 cagtacacga acaacccgtc catgaatatc tgcagacaat accggccatg aaaagataaa
     1801 taataccagt ggaatatatt tcatcatgtt attctccata tcagataaat ggcacctgta
     1861 tttcaggcat cctgaatatt gcagcacaca atgaataacg ccttattttg tgaaataaat
     1921 cacaaaatat aaaatcacga cttaatacgg aaaaaactct gtgctactca gaactgtccg
     1981 gtcatactgc ccgaccggat attaaaaaaa gcaaatctca taaactcctt tcagccttct
     2041 gacttctggt gtaatgaagg ctgctttttt cgttacaccc ttccccggca gcgccggtgg
     2101 cagtgttcag cccttttccg ggcaaactgc tg
//
LOCUS       DAEBXD010000270         2111 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEBXD010000270 DAEBXD010000000
VERSION     DAEBXD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.276"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KJL94_004292"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KJL94_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBD4412374.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="KJL94_004293"
     CDS             complement(983..1813)
                     /locus_tag="KJL94_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBD4412375.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          516 a          481 c          470 g          644 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca g
//
LOCUS       DAEBXD010000271         2096 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEBXD010000271 DAEBXD010000000
VERSION     DAEBXD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.277"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="KJL94_004294"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="KJL94_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBD4412376.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="KJL94_004295"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="KJL94_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBD4412377.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAEBXD010000272         2084 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEBXD010000272 DAEBXD010000000
VERSION     DAEBXD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.278"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..796
                     /locus_tag="KJL94_004296"
                     /pseudo
     CDS             1..796
                     /locus_tag="KJL94_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="KJL94_004297"
                     /pseudo
     CDS             911..1252
                     /locus_tag="KJL94_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="KJL94_004298"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="KJL94_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          504 c          631 g          433 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatccag gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagc
//
LOCUS       DAEBXD010000273         2062 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEBXD010000273 DAEBXD010000000
VERSION     DAEBXD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.279"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..367)
                     /locus_tag="KJL94_004299"
     CDS             complement(<1..367)
                     /locus_tag="KJL94_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD4412378.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="KJL94_004300"
     CDS             complement(406..693)
                     /locus_tag="KJL94_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412379.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KJL94_004301"
     CDS             complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KJL94_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4412380.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="KJL94_004302"
     CDS             complement(1326..1991)
                     /locus_tag="KJL94_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBD4412381.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          532 a          530 c          463 g          537 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gt
//
LOCUS       DAEBXD010000274         2041 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEBXD010000274 DAEBXD010000000
VERSION     DAEBXD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.280"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..181
                     /locus_tag="KJL94_004303"
     CDS             <1..181
                     /locus_tag="KJL94_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4412382.1"
                     /translation="FWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGN
                     ISPAAFREKYHQMAA"
     gene            complement(220..1359)
                     /gene="pdeF"
                     /locus_tag="KJL94_004304"
                     /pseudo
     CDS             complement(220..1359)
                     /gene="pdeF"
                     /locus_tag="KJL94_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
     gene            complement(1541..1693)
                     /locus_tag="KJL94_004305"
     CDS             complement(1541..1693)
                     /locus_tag="KJL94_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412383.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1711..1902
                     /locus_tag="KJL94_004306"
     CDS             1711..1902
                     /locus_tag="KJL94_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBD4412384.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          633 a          462 c          423 g          523 t
ORIGIN      
        1 cttctgggga acgctgaaaa atgagagcct gagccactat cgttttaata accgggatga
       61 agccatctca gtaatacggg aatacattga gattttctac aatcgtcagc gtcgtcactc
      121 tcgtctgggg aatatctccc cggcagcctt cagggaaaaa tatcatcaga tggctgctta
      181 aaaaagaaca aatggtagtg tccgctattg ccagtacacc tcatattgaa tccgcagcat
      241 gatgccatag ttcttaacca gcatttccat gccagggatg cgtaaataac aaagcgcaga
      301 ccagggggca tcacgcaacg cgcgattcag ggcgcgaaca tttggcagat gaaccaccgg
      361 gtccacatac gcaagccgct gaatgcgtct gactaccgct cgctgacgag ttgccagtac
      421 agccatataa ttgacaataa aagagaatac cagataactg gaggaggtta tggtcagctg
      481 cgtggtatag ccaggataaa tgggaatgta attttgatag ctgtggatgc tgatcatcaa
      541 cacgaccgcc cagagcagcg aaatcagctt atagccatag cgcattgctc cccacatcat
      601 caggggcagc aataatgaca aggtataatt ggtgctaaaa attgtgcttt tttcatttaa
      661 cggcatgcat aacagcaaca ataaagcacc taatgccagt agccagagcg cgaactcttt
      721 tttggtgact ttggcatcaa cctgctgttt taattgcgaa taatagctac gtaaataaaa
      781 tggatttcgc actacccgaa tgatgaagta gcacagcggg acaccgatca gattacccac
      841 cagtaaggcc tgataattga ttaaagtccc gaggttaaaa ggcatgacac cgaccagatt
      901 ttctctgctc gccagtaatc ctacaaatgc agcaaactgg aaaagtatca gaaacagcgt
      961 tgcaggaaac actatctgcc agaatatacg ctgggaaatt aaacgggtat caccatgtga
     1021 aacgttgtta cgccggggag taaagaccct gtaaccgccc caacagagaa tgataatgat
     1081 aataaaatta gccgttaatg agagcgtttc gtaaaaaccc agctctgcat atttgcggac
     1141 aaaaatccct aaggtgatcc ctggcaatgc cgcccagctg aaaaacatca tcatgctgat
     1201 catcagtgcc aaaggcagat aaaagagaaa aacctcaccg gaagaaatat gcgcgaaagt
     1261 attaatgtgg gcgaaaatgg gcaagattaa agaaggcagg aacagcggaa gcccccacca
     1321 tttatcacgt atttttatat aagttgcatt cagtttcata gatgctcagc agaatccccc
     1381 acatcctgaa ggaggtgtat tcagacaggc atcccacctg acttcgaatg atgattattc
     1441 atcactatag agagcattga ttctaagtgt catatgaaag ttccaattga tatatatcaa
     1501 acaaaataac cctgattaat gaattattac gtttatcatg ttaattcatc attattacat
     1561 catcattgta aataattaaa ttaacttcca taacattaaa atatgtatcc actgacgctt
     1621 ttttacataa cgaagaattg accattttgt cctgttgtgc cttaatgtaa gtaccgtcca
     1681 cagcgtggga catacttcaa ggaacctttt gtgagtcagg caactagtat gcgaaaacga
     1741 caccgattta acagtcgcat gacccgtatc gtactgctca tcagctttat cttcttcttt
     1801 ggccgtttta tctactcgtc cgtcggtgcc tggcagcacc atcagagcaa aaaagaagct
     1861 cagcaatcca cactctccgt cgaatcaccg gtacaacgtt agcggttacc ttctccactt
     1921 tcacagaaca taacggcact tcgctgtcgg atgcttttgc tctttgggat tatcaaagcg
     1981 gcagatattc tttcatctta aattttacgt ctttgtcctg actgatgttt atcctgtttg
     2041 g
//
LOCUS       DAEBXD010000275         2034 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEBXD010000275 DAEBXD010000000
VERSION     DAEBXD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.281"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>2034
                     /gene="sat"
                     /locus_tag="KJL94_004307"
     CDS             <1..>2034
                     /gene="sat"
                     /locus_tag="KJL94_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBD4412385.1"
                     /translation="YGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGI
                     ASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQE
                     KITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESI
                     VNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKV
                     ILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLA
                     INNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGH
                     ATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWK
                     TGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFD
                     FKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIA
                     QNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVT
                     GNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNA
                     IWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          746 a          359 c          401 g          528 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa cgaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgcaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcag
//
LOCUS       DAEBXD010000276         2028 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEBXD010000276 DAEBXD010000000
VERSION     DAEBXD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.282"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="KJL94_004308"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="KJL94_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBD4412386.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="KJL94_004309"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="KJL94_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            821..2014
                     /locus_tag="KJL94_004310"
     CDS             821..2014
                     /locus_tag="KJL94_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HBD4412387.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          530 a          462 c          497 g          539 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgtccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggatttg gtcttcgggc
     1741 cagcaaaagt cgttcaacag gaagaattct ggttctgagt ctgctggcaa cgttgagcac
     1801 tatcgtaatg tggttactcg gttatcacgc tgaaaataaa gggttacacc tgaaatatca
     1861 ggctaacagc attaaatccc ggcgggttat ctcatatctg acgttagcga agaatgttct
     1921 tcgacactcg ccacttattt taagacgaac agtactgagc acagttctaa accatctgtc
     1981 cagaacctac cgaaatatgg tgctggttta ttagcagtaa ttttgtgg
//
LOCUS       DAEBXD010000277         2025 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEBXD010000277 DAEBXD010000000
VERSION     DAEBXD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.283"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..475)
                     /locus_tag="KJL94_004311"
     CDS             complement(<1..475)
                     /locus_tag="KJL94_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412388.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERF"
     gene            complement(1056..>2025)
                     /locus_tag="KJL94_004312"
     CDS             complement(1056..>2025)
                     /locus_tag="KJL94_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD4412389.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          591 a          384 c          417 g          633 t
ORIGIN      
        1 tgaatctttc gggtattcct tcttctacga gaattttagt tatgattgga ttgttgggtt
       61 ctggattaac gacaacaatg atttgacata cagaatcctt atccttttcg tatctaatgt
      121 tatgaatctg attaagtatt gattgtgtct gttgcgtaag tggtgtcgaa cttatcgttt
      181 caatgttatt aataagactt acaagttgaa ccacaacttt tgagtagtct gcatttgtat
      241 atgtggtgtt cgtgtggcat gcattaattt gttggagttg ttgttgcgct aaatcaaatt
      301 gttcagggtt gtatccttct ccagagaatg tggaattaat gggttgttgt tcttcccaat
      361 tatcacaagg ccacttcggt gtgtctggcg ttttaacatt atcggtattt gttgtgcagt
      421 tataaatatc gccgtgatat gtatttatta tacctggggt aaagtcaatt gacataacat
      481 tccttattca tcatctgttt tatgctaatg tttttaaatt ggataattag aacaggaatg
      541 gattttcttc tacacacaat aaatataatg cgttatatct tcctgatata attaccttta
      601 aattcccttt catttccacc cggtaatatt tgtgtataaa aaaagggcag tgatagtatc
      661 gtaaaggaaa acgttaacaa ctatcaaaaa acgctcattt taaaaagtta ttatgttctc
      721 cgatagtgtg tgaagtaata tttttgtgat gttttagatg tgtaataatg aaccaggtta
      781 aagtgtattt aaaaatgtgt tgtattagcg actgaaatct gccttttaaa aaggaggagg
      841 gtactgagac aaagagtcat tgcttctccg ataaaatgtc agagaagcat gattacgtgg
      901 ttattgtctg ttgatgactt gttgaccgag ccgtcatcat tctcaatgca ttagttaaat
      961 catttgtgat ttttaaggat tatcctgtct atgattttct acttagaagg atatggcact
     1021 aactatgcat cttatagttt actgctggca tctatttaaa atagcaattg agtcaaatag
     1081 cccatcaaga tagccatacc taatataacc gtcaggaatg cagctatcat cggcattctg
     1141 aacattgatt taagtaatat cacttccgtc aggcttgcgc cggcactgcc gatgattaat
     1201 gccattaatg ctcccagacc cattcctttt gtcatcagaa cagatgccag agggataacc
     1261 gcctcagccc ggatatacag cggaataccg acaacggcgc ttaatgggat ggcgaaggga
     1321 ttatctgctc ctgcatgagc ggcaatccac gctgagggaa taaagccata aataaaagat
     1381 cctattaata ctcctaacaa aagatatggc agtacatctt taaactgttg caacgcatct
     1441 ttggtggcta gttttatggg attaatactt actacagctt tggtactgca acaggtaact
     1501 acggggttct caatggcttt agccgctgga ctacaacaac tcacttttat gggcgtatat
     1561 gtcgttcccg gcgaagtttt tgctggagca caacaatttg ctgatgagct tttactggca
     1621 atgatatgac gttcaaaccc cagagaatcc aggataatac tggcaagaac ggagacgccg
     1681 gctgcgataa tcgcgtacaa caaggtgact ttccaaccaa aggtcatcca cattagtccg
     1741 acgataatgg gattaagtaa tggtgaaaca aataaaaaag tgagagtcgg accaaacccg
     1801 gctttcgctg acaacaatcc acgtaacatg ggtattgtcg agcaactaca gaacggggta
     1861 acggctccca acagagcagc caggagatag cctctccctt ttctggcccc catcatctgc
     1921 tggattttat ggtctggcac cttttgtctt atcaggctga caccggcact aatcactata
     1981 aacagcaacg aaagctcgac ggcgagaaac acaaacatct ctgcg
//
LOCUS       DAEBXD010000278         1978 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEBXD010000278 DAEBXD010000000
VERSION     DAEBXD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.284"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..553
                     /locus_tag="KJL94_004313"
                     /pseudo
     CDS             1..553
                     /locus_tag="KJL94_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(595..1125)
                     /locus_tag="KJL94_004314"
     CDS             complement(595..1125)
                     /locus_tag="KJL94_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4412390.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1281..1847)
                     /gene="slp"
                     /locus_tag="KJL94_004315"
     CDS             complement(1281..1847)
                     /gene="slp"
                     /locus_tag="KJL94_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBD4412391.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          558 a          394 c          437 g          589 t
ORIGIN      
        1 tacgcacaca tgtattaatt ggcatgggaa gcgccctgtt tatgattgtt tcgaaatatg
       61 gttttgctga cgtgctgtct ttagatcacg tcggactcga ccccagccgt attgctgctc
      121 aggtggtgac gggcgtcggg tttatcggtg caggtaacat tctcgttcgt aaccaaaata
      181 ttgtaggtct gacgacggca gcggatatct gggtgaccgc cgcataggta tggttattgg
      241 cagcggtatg tacgaactgg gtatttatgg ttcagtgatg accttgttgg tgctggaagt
      301 cttccatcaa ttaaccttcc gcctgatgaa caaaaattac catttacagc tgacattagt
      361 gaacggtaat acggtatcaa tgctcgactg gtttaagcag caaaaaataa aaactgattt
      421 agtttcgttg caggaaaatg aagatcatga agtggtcgcc attgatatcc agcttcatgc
      481 cacaacgttg atagaagacc tgttgcgatt attaaaaggt atggcagggg tgaaaggcgt
      541 ttcaattagc taatctgtgt ggcagcgtag ccagactcac cgtaagcctg aaattcacac
      601 cagataatca atatgctgat ggcgtaccag ctcattgatc cgcttaagat tgaggatcat
      661 catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt tctccagaat
      721 atcttcagtt gtcatttgat tcgcaatgca gctcagaacc tgatgttgct tgcgcgaaag
      781 tgacacattc gcttcaggca cagagcagta actgccattg atgagtgcga caaaatcaat
      841 gatagtgata gtccgcggaa caaaggtcac cggtactttc cccagacagc gtttaatatt
      901 aaagggggag aaaacaatga catggacagg ttttaatcgt tcaatttgag tgagaaaatt
      961 agaatgaaat acattattca ttaatgtatc aataatgacg aaggcattct gatgcaacat
     1021 tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca gaatgttttt
     1081 catcgcggtg aagaacatcg tatccctggt aattataaga aacataaccg actcctggtg
     1141 cggacattaa ctttataatc agtttgccaa aggtaattaa caggacaggg cttataataa
     1201 taatagttag cttactgtat attaatttta tgtaatacat gatgcagatg ctgagaaagc
     1261 atctgcatct ttcggtggtg ttatttgacc agctcaggtg ttacctgact caccgcattg
     1321 gtgtagtaag gcgcacccca gcccggttcc ggccagaatg caccatagcc gtaatcccac
     1381 aggttatagg tggtattaac cacttctctc aaatgccaca cctggatgcc ctgcatattc
     1441 acttccagga agttatacgg gactttattg ataaagcctg gttgttcacc ctgaatggta
     1501 ccgaggatgg taacaaagtg attacgatag ttcactggat caaggaagcc gctttgtctg
     1561 gcgagcagtc ggccctgata gttggcttca atatcaggct tcgcatagct atccaacggt
     1621 aatacagcga tttctaacaa cgtatccgtt ttgccattga taacgttgat aaccttccca
     1681 ccaaagcgcg cttgttgacc aacatataac cccggctggt tatgaacagc aacaaaactt
     1741 ttttgaatat caggttggtt attgcctttg atattttgcg gaattgaact acatgcggca
     1801 agcaaaaatg aaaggctgag gatgagtgca ccttttgtca tgttcatgtt actatcctta
     1861 tcaacaaata ttaaaaccat aatagatatt tctttatagt tttcatctga ttctgaggtg
     1921 atatgcctga tagtaacgta aacctgtctg atgtttatat cccttctgtt tagttcaa
//
LOCUS       DAEBXD010000279         1938 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEBXD010000279 DAEBXD010000000
VERSION     DAEBXD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.286"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..950)
                     /locus_tag="KJL94_004316"
     CDS             complement(<1..950)
                     /locus_tag="KJL94_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBD4412392.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGTFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRSWNVNVSRVMDNATHVAVCFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVF"
     gene            complement(950..1315)
                     /locus_tag="KJL94_004317"
     CDS             complement(950..1315)
                     /locus_tag="KJL94_004317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412393.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            1400..1938
                     /locus_tag="KJL94_004318"
                     /pseudo
     CDS             1400..1938
                     /locus_tag="KJL94_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          493 a          502 c          476 g          467 t
ORIGIN      
        1 aaaaaaacgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatggact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagcacacc gccacatggg tggcattatc catcacccgg
      241 gatacgttga cattccagct acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtcagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atgtccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt cggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
      721 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
      781 tccggtgatg aacaacacca ctgcgtgtag cccatcagtg tggtcccgca ggccatgatt
      841 tttgtcactg attcaattac caccggacgg actgcccctt ccggttgttt ctccagccag
      901 ttaagccagc ggtttccctg ctgaaaaata tcggcaaaac gggggagcat cagaagggcg
      961 gggcgactcc gtccggccag tgaaccgtgc cacactccgg gcagtacata ccgccggcgc
     1021 tgataccggc aagaatggtc gcaaattccc gctccgtgca gcgggcgatt tccggatacc
     1081 cttcgtcatc aacacgtaca aaccagaaga ccagcttttt gtttcccgca tccacaaaga
     1141 acggaatatt caggtctgcg cagcattcaa cggcatcgtc aaaactatca aagcgcagaa
     1201 cttctgcgtc ttcttcgtca aaaaaatcat cttcgtgaag cttcacgaca tagcggggaa
     1261 gtttgcttct ttgagaggcg ggtttacgtt tacggggttt agctgaacgg gccatataac
     1321 cacctgaaag acaatgacat tgcctgtttt tataacggta attgcagacc atgacaagcc
     1381 gcagccgtca ggctgcctac tcgataattt aagttcagca gcagttaaaa cagcaggtgt
     1441 acttatcagc tttcaggctg tactatccgc ttatcagcga gtaatggaag ttggccttca
     1501 acgtgcaagt gctgaacgtt caattgattt cgtattttct aaagatgcaa atcaagtaaa
     1561 agatttcaca aagtcattag ctcaaacaac gggtttagat atcgctgaac tgcaatcaca
     1621 gtttgctggt ttcggtgctt cagctagaga gtctctaggc attcaaggtt ctgaagaact
     1681 ttttagaaat atgattggct attctcgtct gatggggcga agtgaagaag agattaagcg
     1741 agcactgaca gcattatcac agatggctgg taagggtcag attatggctg aagagcttaa
     1801 ggggcaacta gctgaagctg ttcctggtat ggttcaggta ttcgctaaag caaccggaaa
     1861 gactgaacag gaattatttg atgctatgaa gaagggggct ttaaaatctg ccgatactct
     1921 tcagaaggtc actcagga
//
LOCUS       DAEBXD010000280         1919 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEBXD010000280 DAEBXD010000000
VERSION     DAEBXD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.287"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(226..429)
                     /locus_tag="KJL94_004319"
     CDS             complement(226..429)
                     /locus_tag="KJL94_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412394.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="KJL94_004320"
     CDS             complement(431..586)
                     /locus_tag="KJL94_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBD4412395.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="KJL94_004321"
     CDS             complement(789..1196)
                     /locus_tag="KJL94_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4412396.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="KJL94_004322"
     CDS             1273..1500
                     /locus_tag="KJL94_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4412397.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="KJL94_004323"
     CDS             1484..1906
                     /locus_tag="KJL94_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412398.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
BASE COUNT          468 a          414 c          492 g          545 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcg
//
LOCUS       DAEBXD010000281         1915 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEBXD010000281 DAEBXD010000000
VERSION     DAEBXD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.288"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            193..462
                     /locus_tag="KJL94_004324"
                     /pseudo
     CDS             193..462
                     /locus_tag="KJL94_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="KJL94_004325"
                     /pseudo
     CDS             459..685
                     /locus_tag="KJL94_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="KJL94_004326"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="KJL94_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBD4412399.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1643..1915
                     /locus_tag="KJL94_004327"
                     /pseudo
     CDS             1643..1915
                     /locus_tag="KJL94_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          539 a          415 c          455 g          506 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgagagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcagcaaggc gaatcacgca cggatttcca gccagagaca cctgaaacgt
     1561 aaccggctca tttaaaccgt ctggtctgtt acctccggtt ccacaaaaat aatgtccatc
     1621 attttgaagt ggacactatc gtatggaata ccggacctga atcactgaag ctttacgcct
     1681 tcatttcgaa gaacatttac cacgggttgt ggccggatgt cgcctgggcg taccaaaatc
     1741 aactgtctgt ggtatgttcg tgcgcttccg caacgctggc ctttcatggc ctctgcccgc
     1801 aggtatgtcg gagcaggaac ttgatgccct tctttacgga agtgcctcca cagtacctgt
     1861 cgtacttact gaaagcaccg ttatgcccaa actcccggtg gtaaaaaaac gtccc
//
LOCUS       DAEBXD010000282         1913 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEBXD010000282 DAEBXD010000000
VERSION     DAEBXD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.289"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(53..1702)
                     /locus_tag="KJL94_004328"
     CDS             complement(53..1702)
                     /locus_tag="KJL94_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD4412400.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAEBXD010000283         1882 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEBXD010000283 DAEBXD010000000
VERSION     DAEBXD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.290"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(117..386)
                     /locus_tag="KJL94_004329"
                     /pseudo
     CDS             complement(117..386)
                     /locus_tag="KJL94_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(501..1139)
                     /gene="ospI"
                     /locus_tag="KJL94_004330"
     CDS             complement(501..1139)
                     /gene="ospI"
                     /locus_tag="KJL94_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBD4412401.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1298..1768)
                     /locus_tag="KJL94_004331"
                     /pseudo
     CDS             complement(1298..1768)
                     /locus_tag="KJL94_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          536 a          464 c          341 g          541 t
ORIGIN      
        1 cgtttctccg gccctaagac atcaatcatc tgttctccaa tgactagtct aaaaactagt
       61 attaagacta tcacttaaat aagtgatact ggttgtctgg agattcaggg ggccagtcta
      121 cccagcaagc actgtaactc ttttatttgt tttatagcgg cagccagttc tgaggcagga
      181 acgacatcct ctccggcagc aacagcagtg agacttcctt ccagatatta ttttttccac
      241 ttaagcaaca ggctgggctg aataccatgc aaacgcgcca catgggacac cgtcatgcca
      301 ggctccattg tctgctgaat aatggcaatt ttttcctgag gagttttacg tttacgaagc
      361 tccagcccca gcaaagttcc agtcatctca aaattagaac tagtgttaga catatatcca
      421 agcctatctc ttattttaag atacatccac tgtctggcgt ttcaaggggc cggctcaaat
      481 ctatgtacag gctctcagca tcagcaaagc ctcttacttt tccaactact actgttagca
      541 tagtgatcaa attcctgatc agatacatga attacctgaa ccgaactatt ttcatctaca
      601 aatgcatctg gcgatttttc tacaggatca taaagattaa atccctttcc tgtttgaaca
      661 tccaaaaaca taattttttc accatcgtta aatccagcta tccaatgagt ggcctgttca
      721 atagaaatca ttactacact tccttttgaa gtattttgta aaatattttc tgcaatacgc
      781 tcacgaaatt tactataagt ttcattagga tgtgacacta tgccttcact atcaatttgc
      841 tctaaatgcc tatgaaaaaa tccggtcata tactctgata tttcattagg tacgggagcc
      901 tctcttactc cataccctaa catagccata caagcatgaa gcgcacaccc tgaacagtta
      961 ccatccccac cagacggatt tatcattttc tgtaaagtcg ctgccaattc agaatcttta
     1021 tccatatcac caagactttt agagtttaaa gattccagca attttttaac atttacattt
     1081 ccctcctctg tatgtgcctc atatccagca gttccctgta acgacacccc attaatcata
     1141 ttgtatcctg acagtatata tactcactat cttttcttca taacaaatat ccctctgata
     1201 tgacaacaaa aaacggagcc ggattcctgt ttttgtgaac tgcagctatt cgtgtctaaa
     1261 aagtgatcca cttaccacca acggcctaat atatacagaa cggagtaaac aggactaaca
     1321 gttcccgaca ggtctcatca ttccgtggcc cgaaagtgta agccaacaca ccgccagttt
     1381 tagtgttgta cgcataccag agccagtgtt gttgcagcct tgctgccaac gaagcgccac
     1441 tgctcatcaa gttcacagat aagggcaaca tcagcgtgag cgactggcgc agatgttatc
     1501 cgccggggcg ggagcttttt aatgtccgta tgacggtatt gatgccgatt ttcagtgtcc
     1561 tggcggtatc acggccccct gcgccgttat gtgccatttc aacgatctgc tccttaacgc
     1621 cgggttttct ggcttcataa gagtaagtga gctggaaaac acgtttacat gagcgacaac
     1681 ggaaacgctc atgccgggaa caactacggc catgtcgata aacttcatcg gaatgacagc
     1741 gcggacaatg aacagtaacg gtagccatgg aaaaccttaa ataccagtag attaccagaa
     1801 acttctgtcg attgaagaca tcacccgaag tttgaattca ttcgaatact ttttacgagg
     1861 ttcttttcgc cagtcctgta at
//
LOCUS       DAEBXD010000284         1877 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEBXD010000284 DAEBXD010000000
VERSION     DAEBXD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.291"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(235..636)
                     /locus_tag="KJL94_004332"
                     /pseudo
     CDS             complement(235..636)
                     /locus_tag="KJL94_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1065..1853
                     /gene="virF"
                     /locus_tag="KJL94_004333"
     CDS             1065..1853
                     /gene="virF"
                     /locus_tag="KJL94_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBD4412402.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          580 a          338 c          351 g          608 t
ORIGIN      
        1 atgatatttt cttgtttttg tggaaggcaa gggaatatag tttgtgtaag catattgctt
       61 ccttatgtta tattttagta aagaatttat tttttacatt atagtcggct aaatgaagtt
      121 tactgtaaaa aaacgatact tttttcgtaa gcgatgagat gtggagccag aaaaatggct
      181 gcgttacgtc attgaactgt ccttgaccca cgatattcag acagtcgttg tatcttaagt
      241 tgacattacc cgctgacgtc gatattccct tggtgagaga taacccaacg cactatgcgg
      301 atggttttca ttgtaatgct cgatcgccac tgcaaggtta tgcaatgccg ttcttggttt
      361 cggcatgaac gcgatgcagt cttccttcat cgttttcatg aaccgttctg ctattccatt
      421 actctgaggg ctactgactg ccgtcgtgca gggctccaga ttcaactctc tggcgaactg
      481 ccgtgtttca tgagccgtat aagccgaacc gttatcggcc actggattgg ccgttccggt
      541 cctttatcgc catatcgctc ctgacatgac atcctgcacc gttgctttgt cataatctcc
      601 cgtgcctgct gcccagtcta tggcttcacg atcgcaatat ggaacgtaac gctgtcatcg
      661 ggagaatcgt caatgcggca ttcggttaac gcttaccaat atcaacacca accggatttg
      721 tggagcctcc agtctgaagg ggctttatgc gttccgtata ggatattatg atgctggagt
      781 tttgcgaagc tccactcctt tccgaacatt tacacagata ttgcctaaga aaagtgaaac
      841 cataatatgg ttatagtccc tttcagtgca aatacttagc ttgttgcaca gagaaataga
      901 agctgcataa gctctttctt caaaaaatgt aaataaagtt aaatatagga aaaattactt
      961 aatctatctt aataacggaa agattttgta tacaatcact gttacagaaa tttcttagtt
     1021 actctgtaaa cactaaatat agtttggtat attctgttga atttatgatg gatatgggac
     1081 ataaaaacaa aatagatata aaggttcgct tgcataacta tattatttta tatgcaaaaa
     1141 ggtgttcaat gacggttagc tcaggcaatg aaactttgac tatcgatgaa gggcaaattg
     1201 cttttataga gcgaaatata caaataaacg tctccataaa aaaatctgat agcattaatc
     1261 catttgagat tataagcctt gacagaaatt tattattaag cattattaga ataatggaac
     1321 caatttattc atttcaacac tcctattctg aggagaaaag ggggttaaac aaaaaaatat
     1381 tcctcctctc tgaggaggag gtttctatcg atttgttcaa atctataaaa gagatgcctt
     1441 tcggcaaaag aaagatctat agtttagctt gccttttatc agctgtttct gatgaggaag
     1501 ctttatatac ttcgatatcg atagcttctt ctcttagttt ttctgatcag ataaggaaga
     1561 ttgttgaaaa aaacatcgag aagagatggc gtctttctga tatttcaaat aacttgaatt
     1621 tatcagaaat agctgttaga aaacgattgg agagtgaaaa attaacattt caacaaatcc
     1681 ttcttgatat tcgcatgcat catgcagcaa agcttttatt gaatagtcaa agctatatta
     1741 atgatgtatc aagacttatc ggaatatcaa gcccatctta ttttataagg aaatttaatg
     1801 aatattatgg tataactcca aagaaatttt acttatatca taaaaaattt taaatgcttc
     1861 atagcccatc gctattg
//
LOCUS       DAEBXD010000285         1858 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEBXD010000285 DAEBXD010000000
VERSION     DAEBXD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.292"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..206
                     /locus_tag="KJL94_004334"
     CDS             <1..206
                     /locus_tag="KJL94_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412403.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="KJL94_004335"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="KJL94_004335"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBD4412404.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          451 a          465 c          439 g          503 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgg
//
LOCUS       DAEBXD010000286         1853 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEBXD010000286 DAEBXD010000000
VERSION     DAEBXD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.293"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            29..400
                     /locus_tag="KJL94_004336"
     CDS             29..400
                     /locus_tag="KJL94_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4412405.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(410..1573)
                     /locus_tag="KJL94_004337"
                     /pseudo
     CDS             complement(410..1573)
                     /locus_tag="KJL94_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
     gene            complement(1588..>1853)
                     /locus_tag="KJL94_004338"
     CDS             complement(1588..>1853)
                     /locus_tag="KJL94_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4412406.1"
                     /translation="ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          487 a          387 c          431 g          548 t
ORIGIN      
        1 ttctcgctat caaaataaag ctaataacat ccaattaaga tatgatgata agcagtttca
       61 tacaacggtt atcaaagatg ttctattatg gattgaacat aatttagatc agtctttact
      121 gcttgatgat gtggcgaata aagcgggtta taccaagtgg tattttcagc ggctgttcaa
      181 aaaagtaaca ggggtcacac tggctagcta tattcgtgct cgtcgtttga cgaaagcggc
      241 tgttgagttg aggttgacga aaaaaactat ccttgagatc gcattaaaat atcaatttga
      301 ttcccaacaa tcttttacac gtcgatttaa gtacattttt aaggttacac caagttatta
      361 tcggcgtaat aaattatggg aattggaggc aatgcactga gagatcccct cataatttcc
      421 ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt
      481 cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg
      541 agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct
      601 gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga
      661 tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac
      721 ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt
      781 gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc tcctttgccg
      841 acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt
      901 ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag
      961 tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga
     1021 taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta
     1081 accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac
     1141 tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt
     1201 catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag
     1261 aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc tcacgaatat
     1321 cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc
     1381 aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc
     1441 gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag
     1501 tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg agtcgtttta
     1561 agtgtaattc ggggtaatga ctccaactta ctgatagtgt tttatgttca gataatgccc
     1621 gatgaccttg tcatgcagct ccaccgattt tgagaacgac agtgacttcc gtcccagcct
     1681 tgccagatgt tgtctcagat tcaggttatg ccgctcaatt cgctgagtgt aacgcttgct
     1741 gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca
     1801 taccacgacc tcaaaggccg acagcaggct tagaagacgc tccagtgtgg cca
//
LOCUS       DAEBXD010000287         1850 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEBXD010000287 DAEBXD010000000
VERSION     DAEBXD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.294"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..258)
                     /locus_tag="KJL94_004339"
     CDS             complement(<1..258)
                     /locus_tag="KJL94_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412407.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="KJL94_004340"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="KJL94_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBD4412408.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="KJL94_004341"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="KJL94_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBD4412409.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="KJL94_004342"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="KJL94_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4412410.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1469
                     /locus_tag="KJL94_004343"
     CDS             1254..1469
                     /locus_tag="KJL94_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412411.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            1572..1850
                     /locus_tag="KJL94_004344"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="KJL94_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          409 c          467 g          467 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ttatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatctgaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa
//
LOCUS       DAEBXD010000288         1833 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEBXD010000288 DAEBXD010000000
VERSION     DAEBXD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.295"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..180)
                     /locus_tag="KJL94_004345"
     CDS             complement(<1..180)
                     /locus_tag="KJL94_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD4412412.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="KJL94_004346"
     CDS             complement(321..875)
                     /locus_tag="KJL94_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD4412413.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="KJL94_004347"
     CDS             complement(872..1162)
                     /locus_tag="KJL94_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD4412414.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="KJL94_004348"
     CDS             complement(1162..1761)
                     /locus_tag="KJL94_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD4412415.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa tac
//
LOCUS       DAEBXD010000289         1825 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEBXD010000289 DAEBXD010000000
VERSION     DAEBXD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.296"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..1825
                     /locus_tag="KJL94_004349"
                     /pseudo
     CDS             1..1825
                     /locus_tag="KJL94_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          402 a          487 c          601 g          335 t
ORIGIN      
        1 ctgataaccg gacgtcgtca gttgctggaa ggaatggcct tcacgccggg cagtaccgtt
       61 atcgtcgacc agggcgaaaa actctccctg aaagagacgt taaccctgct ggatggtgcc
      121 gcacgtcata acgtacaggt cctgataacc gacagcgggc agcgaaccgg tacaggcagt
      181 gcactgatgg ccatgaagga tgccggggtg aacacatatc gctggcaggg gggagaacag
      241 cgaccggcca ccatcatcag tgaaccggac cgtaatgtcg gctggccgga gattttgcgg
      301 ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag cggagtacgg gaacaggcca
      361 tactgacaca ggccattcgc agtgagctga aaacacaggg cgtgctcgga cacccggagg
      421 taaccatgac cgccctgtca ccggtctggc tggacagccg gagccgttat ctgcgggata
      481 tgtaccgccc gggaatggtg atggagcagt ggaacccgga gacacgcagt catgaccgct
      541 atgtgacaga acgtgtgacg gcgcagagtc acagcctgac cctgcggaat gcgcagggcg
      601 aaacgcaggt ggtgcgtatt tcttccctgg acagcagctg gtcgctgttc cggccggaaa
      661 aaatgccggt ggcagacggt gagcgtctga gggtgacagg gaaaattccc ggactccgcg
      721 tctccggcgg tgaccgcctg caggtggcat ccgtcagtga agatgcgatg acggttgttg
      781 tgccgggacg ggctgaaccg gccaccctgc ctgtgagcga ttcaccgttc acggcactga
      841 agctggagaa cggctgggtg gaaacgcccg ggcattccgt cagcgacagt gcgacggttt
      901 ttgcctccgt cacacagatg gcaatggaca atgccaccct gaatggtctg gcccgcagtg
      961 gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg tactgcggaa aaacttgccc
     1021 gccatccctc ctttacggtg gtttctgagc agataaaggc gcgtgccggt gagacattgc
     1081 tggaaaccgc tatcagtctg cagaaagccg ggcttcacac gccggcacag caggccattc
     1141 atctggccct tcctgtgctg gaaagtaaaa aactggcctt cagcatggtg gacctgctga
     1201 cagaggcgaa gtcgtttgct gcagaaggaa ccggttttgc tgacctggga ggggaaatca
     1261 atgcgcagat aaaacggggt gatttactgt atgtggatgt ggcaaaaggc tatggcacag
     1321 gcctgctggt ttcccgtgcg tcgtatgagg cagaaaagag cattcttcgc catattctcg
     1381 aaggtaagga ggcggtcacg ccgctgatgg agagagtacc tggcgaactc atggagaaac
     1441 tgacatcagg acagcgcgcc gccacccgta tgatactgga aacgtctgac cgtttcacgg
     1501 tggtgcaggg ctatgccggt gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg
     1561 tgaacatgct gccggagagt gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg
     1621 cggtcgggga gatgcgcagc gccggcgtgg atgcacagac actggcgtcc tttctgcatg
     1681 acacgcagct gcagcagcgc agcggagaaa cgccggattt cagcaacacg ctgttccttc
     1741 tcgatgagag ctctatggtg ggcaataccg acatggcacg ggcatacgcc ctgattgcgg
     1801 ccggtggcgg tcgggctgtg gccag
//
LOCUS       DAEBXD010000290         1818 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEBXD010000290 DAEBXD010000000
VERSION     DAEBXD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.297"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(1..326)
                     /locus_tag="KJL94_004350"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="KJL94_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(326..1246)
                     /locus_tag="KJL94_004351"
     CDS             complement(326..1246)
                     /locus_tag="KJL94_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4412416.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1384..>1818
                     /gene="pgrR"
                     /locus_tag="KJL94_004352"
     CDS             1384..>1818
                     /gene="pgrR"
                     /locus_tag="KJL94_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD4412417.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          453 a          437 c          422 g          506 t
ORIGIN      
        1 atgcgttcag catcaacata tggcagagtt actgcataat cgataacacg gctaatatcc
       61 tctacgcgct gtgtcggatc ttctatccaa cgaggctacc caccagattc tccctggaaa
      121 ctggcgtcat aagcgagaac gacatagcct ttttcagcca atgccttgcc atagatattg
      181 ccagacgtct gctccttaca actgccaaaa ggatggacgc tgataatcgc cggatactgt
      241 tttgactcat caaaatttgg tggaaactga atattggcag caatatgcca ggccatatca
      301 ctattgcgga tttgaatatg tttcattaca gagtttcctc aaagaacgga gctaatactg
      361 aaattgcttc agccacatag gctttgccgt cataaagatc catatggttt gcgccttcaa
      421 caatgtgata gcgtttatcc tggcttgatg cccgatcgta caggtcgtca ctcatccatt
      481 tgctccctgc ctggctgccc gccacaatct gcatcggctg agtcaggtac acttccgcca
      541 tatggtaagc atcataggta ataatctggt taaggctgcg caaagtagcg taacccggtg
      601 ctgttggata ctgcgcacga ggtgtgtggt aatactccca ggcctgacgc agttcttcgt
      661 tcggcgcatc ggcctccttc attggagcca gtggcataat ggcgtattct tcgctgctaa
      721 tatcgctggt tcgggcgttt gagccagctt caatgtgtgg cagagcatca atagatttca
      781 cattgttttc ccaaccattg cggaacatcg aaccaatatt gaccgcacta acggtaccga
      841 tggccttgat gcggcgatcc tgaattgcag cattggctgt atatcctgca ccggcacaaa
      901 ttcccatcgc accaattcgg gtattgtcga cataagaaag cgttgtcagg taatcaatca
      961 cggcactgac gtcttcagta cgaatgtatg ggttttctaa ctgacgcggc tcgccgccac
     1021 tttcaccttg ataagatgcg tcataagcaa tagtgacaaa tcccttttcc gccagctttt
     1081 cggcataggt tccggccgtt tgctctttaa cgcccccacc tgggtgagat accacaattg
     1141 ccggatactg acgggtttca tcaaattttg gagggaaata gatcactgca gacaaagaga
     1201 tggttggatt attgctgtta gtgaagctga ctttattatt catcattcag ttcctcatgg
     1261 agttgtcggt tcgttttgac ggttggtgat atcactatag atattgatca ttaagttgat
     1321 tagaccctaa atcatgatta gacctatcat ttaaatgatt aatagattgt atggagatac
     1381 tgaatgaagc gtgaagaaat cgctgatctg atggcgtttg tcgtcgttgc agaggagcgt
     1441 agcttcactc gcgcagcagc ccgcctgagc atggtgcagt cagctttaag ccagatagtg
     1501 cgccgtatag aagaacgatt gggattgcgg cttctgacgc gaaccacgcg cagcgttgta
     1561 cccactgaag cgggcgagca tcttttgtct gttcttggcc ctatattgca tgacatagat
     1621 tctgccctga catccctgag cgatctgcag aaccgcccat ccgggacaat acgtattact
     1681 actgtagaac atgcagcaaa aacgatattg ttaccagcca tgcgcacatt cctgaaatcg
     1741 catcctgaaa ttgatattca gcttaccatt gattatggtt tgaccgatgt cgtttctgaa
     1801 cgttttgatg caggcgtc
//
LOCUS       DAEBXD010000291         1813 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEBXD010000291 DAEBXD010000000
VERSION     DAEBXD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.298"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..296
                     /locus_tag="KJL94_004353"
     CDS             <1..296
                     /locus_tag="KJL94_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412418.1"
                     /translation="VSCFSDIPFVREHRIKLIMAGFLVYFWFFSFLVGWYKPQLKKEY
                     QMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRFLIFNTEQSGFYEINDVYVRK"
     gene            848..1252
                     /locus_tag="KJL94_004354"
     CDS             848..1252
                     /locus_tag="KJL94_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412419.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1315..1813
                     /locus_tag="KJL94_004355"
                     /pseudo
     CDS             1315..1813
                     /locus_tag="KJL94_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          510 a          404 c          450 g          449 t
ORIGIN      
        1 ctgtatcatg tttttcagat atcccttttg ttagagaaca ccgcattaag ttaattatgg
       61 ctggtttttt ggtttatttt tggttttttt cctttcttgt tggctggtat aaacctcaat
      121 taaaaaaaga atatcagatg ctttgttata ataatagttg gtattatgtt cttgctcgtt
      181 atgattcacg gctcgtttta tctagttcat ttaaagatga cagtaacaga ttcttaatat
      241 ttaatacaga acagtcggga ttttatgaaa tcaatgatgt ttatgtcaga aagtaataat
      301 cctctcccac aaagtgaatt aaatgaatat ataatgcgtt cgaagtatat gtattgagct
      361 gtcatactga gaaacaaacc ttagggcagc acaattaaag ccagaaacaa ctatgcaaga
      421 aagttactaa ataagtgctc ggaacagtaa aacactcagg ggggactcag tccggattat
      481 gtaatcagat aaattgccaa acatcacaaa tcaccagccg gactgagtga tgccgagcat
      541 tgcaccaata ccccgtgatg aacgatgcct gatgcagaaa gctccctata aaacaaagaa
      601 caaaaactac gaccggcgat tcaccgtcat actgatgctg catcgtggtg acactgtctg
      661 tcactttcat gttgactgaa caactctgct gcgaagaagg tgtaccgccg gcgggatttg
      721 attgtccggc gtctagcttc cacttccctg atggaactga aagttgttat ggccggtcag
      781 cttgccctgt ccgcaggaca tgaggtgctg tgacggacag caccagtaac ggaaagtgac
      841 aatgaaaatg aatactgcgc tgatagttgc actaatgtgt atgtggtatg cggtacccgc
      901 tgctgcaaaa gagactttac tggctatgcc acgtaattca acagaacatt gctatgcgga
      961 gattaatgtg cacggaccat atggggtgta tttccgagtg gtcccgcatc cccccggggg
     1021 aaagagctgg gtcgaatgta acagtgacta ctattactct gataaaccac cgggagtaca
     1081 gatactcgga acccgtgcag ggtgtcgggt atacggaatc tgcgggacca cctcgacact
     1141 gcatgtggct ggaagagggg ttgtgtgtat taaaaatata tgcagtccac gtgggatgat
     1201 aattcacagg atacggaaac gtcctgtagt ggctgtaagc gatgagatgt gatcttaccc
     1261 agcaatagta gacacgcggc taagtgagta aactctcagt cagaggtgac tcacatgaca
     1321 aaaacagtat caaccagcaa aaaaacacgt aaacagcatt cgcctgaatt ttgcagtgaa
     1381 gccctgaagc ttgctgaacg catcggtgtt gctgccgcag cccgtgaact cagcctgtat
     1441 gaatctcagc tctatgcgtg gcgcagcaaa ctgcagcaac agatgacctc gtctgagcgt
     1501 gaaagtgaac tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
     1561 tccaaaaggc cgcgacatac tttgcgcagc ggctggtata cgtggtgtca gcgtcggaca
     1621 ggaataagcc ctcgtcagca gttccgtcag cactgcgaca gcgttgtcct cgcggcggcc
     1681 tttacccggt caaaacagcg ttacggtgca ccacgcctga cggatgaact gcgtgctcag
     1741 ggttaccact ttaacgtaaa aaccgtggcg gcaagcctgc gccgtcaggg gctgagggca
     1801 aaggcctccc gga
//
LOCUS       DAEBXD010000292         1810 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEBXD010000292 DAEBXD010000000
VERSION     DAEBXD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.299"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..234
                     /locus_tag="KJL94_004356"
                     /pseudo
     CDS             1..234
                     /locus_tag="KJL94_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="KJL94_004357"
     CDS             655..1233
                     /locus_tag="KJL94_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412420.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="KJL94_004358"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="KJL94_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1810
                     /locus_tag="KJL94_004359"
                     /pseudo
     CDS             1584..1810
                     /locus_tag="KJL94_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          587 a          322 c          424 g          477 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc
//
LOCUS       DAEBXD010000293         1805 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEBXD010000293 DAEBXD010000000
VERSION     DAEBXD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.300"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            173..1198
                     /locus_tag="KJL94_004360"
     CDS             173..1198
                     /locus_tag="KJL94_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4412421.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1805
                     /locus_tag="KJL94_004361"
                     /pseudo
     CDS             1266..1805
                     /locus_tag="KJL94_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          461 g          503 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgc
//
LOCUS       DAEBXD010000294         1795 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEBXD010000294 DAEBXD010000000
VERSION     DAEBXD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.301"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..339)
                     /locus_tag="KJL94_004362"
     CDS             complement(<1..339)
                     /locus_tag="KJL94_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412422.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQY"
     gene            complement(923..1331)
                     /locus_tag="KJL94_004363"
                     /pseudo
     CDS             complement(923..1331)
                     /locus_tag="KJL94_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1355..1795)
                     /locus_tag="KJL94_004364"
                     /pseudo
     CDS             complement(1355..1795)
                     /locus_tag="KJL94_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          512 a          425 c          349 g          509 t
ORIGIN      
        1 atattgtttg agaattaaaa gttcgaatat tacagggaaa aacagtaggg taatagcaaa
       61 aaatagtcta aaaaaactca agtcttgtat tttacttctt ttctttataa gaagaaaaaa
      121 taaaatacca atcccccaac cggtaaaaag tatacccatt actatgatag catagagtaa
      181 acttctggca acatcatcca aacctgcacc aacaacccac catggaaatc catagtaaaa
      241 agaggttccc cagccataag aataagcact gacccaccct acacatccca tataggcgat
      301 gaataatgaa gaatttctaa gcaatgaatt gtcattcata agaaaccaac tgataatgta
      361 aaaaatattc gagataaaag ttgatctttt aattttcatg ctaggaaatt aacaataata
      421 tcattgttaa tgcatattta tcatatcaaa atactctgca gaatgctact atcaaaacca
      481 gacaagtgaa caaaacttgg tcaacattta gtaacgctag ttggtaatgt cttcaatcga
      541 tagaagtttc tggtaatcta ctggtattca aacagttagg gctcatacag cttgctggac
      601 cttccccata accgcccgaa cgtgaaggtc tgatgtgttg caagagagcc ggatgcggga
      661 aatctgcaag tccggttcga tgagagggac gttgttttta cttgttgttg atgaagactt
      721 tcattattga acagaatgat aatattgtct aaacaaaaca ttaaatctca taattctttg
      781 tagaaaacgc ttttcttgaa attaaaaata agtagaatca gcagtctcat tataataaca
      841 gacaatgtca tgctctcgcc cgttccggta ataaacggag cgggctatct ttttggaata
      901 tttatggccc acaatccagt tttcataaat acgattcatt ccattcttcg ttttttctga
      961 gcattgcgtt cataatggtc agcaacttac gcatacaggc aaccagtgcc acttttttgg
     1021 cgtttccggc tgcaagtagg cgcatgtaaa acgcctttat caccagatta aaccgggggg
     1081 gcctcaagcg ccgccatgta cagagctgtt cttccccagc acaatcaccg aaaatggttc
     1141 gtcgaccgcg cagagttccc gagtcccggt ttacctgagc aacaccgaca agtgcgctga
     1201 tttcacgtct cgagaggctg ctcgattcag gcatttccgc cagcaatgct gcggcagtca
     1261 tggctctaac acctcagacg ttcagtaagc tctttaaagt aactctgaat gtgcctgttc
     1321 atatctgggt cttccccgat catggtggga aggcttagaa cgctatattc agtttcccat
     1381 agtggaacat cactcccagc ttaaagcgct cccggttgcg gtagcccttg gcttttattc
     1441 tcagcagcct gatcttgctg ttcagtgctt ccgcatttcc gtttgatacg ccatggcgca
     1501 tggcgttcag gataccgtac aaccttttcc ctatcgtttt agccacattc ttcatcatgg
     1561 ggacgtcact gttggccgca gccatctctg ccagtcgttg cgtctttcct cgctccatgg
     1621 tctgctccag atatcctttg ccagttcttt cagcgcccag cacaggctcg tcagcttcat
     1681 ctgtgcacgc agccatatca gcttttcctg cctggattcg gtcatccatt tatcgctgtg
     1741 ctgccacagg aagcgagttc cctttgcctg acgacgactt tcaacaggaa gatgc
//
LOCUS       DAEBXD010000295         1791 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEBXD010000295 DAEBXD010000000
VERSION     DAEBXD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.302"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..363)
                     /locus_tag="KJL94_004365"
     CDS             complement(<1..363)
                     /locus_tag="KJL94_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4412423.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="KJL94_004366"
     CDS             complement(430..1644)
                     /locus_tag="KJL94_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBD4412424.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          530 a          469 c          373 g          419 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac c
//
LOCUS       DAEBXD010000296         1783 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEBXD010000296 DAEBXD010000000
VERSION     DAEBXD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.303"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            53..1783
                     /locus_tag="KJL94_004367"
                     /pseudo
     CDS             53..1783
                     /locus_tag="KJL94_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          468 a          402 c          445 g          468 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccactcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taa
//
LOCUS       DAEBXD010000297         1725 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEBXD010000297 DAEBXD010000000
VERSION     DAEBXD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.304"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..243
                     /locus_tag="KJL94_004368"
     CDS             <1..243
                     /locus_tag="KJL94_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD4412425.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="KJL94_004369"
     CDS             complement(332..844)
                     /locus_tag="KJL94_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBD4412426.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..>1725
                     /locus_tag="KJL94_004370"
     CDS             1039..>1725
                     /locus_tag="KJL94_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412427.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGI"
BASE COUNT          506 a          386 c          437 g          396 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtatt
//
LOCUS       DAEBXD010000298         1706 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEBXD010000298 DAEBXD010000000
VERSION     DAEBXD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.305"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            43..222
                     /locus_tag="KJL94_004371"
     CDS             43..222
                     /locus_tag="KJL94_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412428.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="KJL94_004372"
     CDS             274..468
                     /locus_tag="KJL94_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412429.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="KJL94_004373"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="KJL94_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          514 a          382 c          359 g          451 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttc
//
LOCUS       DAEBXD010000299         1683 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEBXD010000299 DAEBXD010000000
VERSION     DAEBXD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.306"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KJL94_004374"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KJL94_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBD4412430.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1683
                     /locus_tag="KJL94_004375"
     CDS             1494..>1683
                     /locus_tag="KJL94_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBD4412431.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLR"
BASE COUNT          484 a          286 c          396 g          517 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gtt
//
LOCUS       DAEBXD010000300         1672 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEBXD010000300 DAEBXD010000000
VERSION     DAEBXD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.307"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            105..1307
                     /gene="virA"
                     /locus_tag="KJL94_004376"
     CDS             105..1307
                     /gene="virA"
                     /locus_tag="KJL94_004376"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBD4412432.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            1486..>1672
                     /locus_tag="KJL94_004377"
     CDS             1486..>1672
                     /locus_tag="KJL94_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412433.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          592 a          338 c          279 g          463 t
ORIGIN      
        1 tcgttttctt aaagagaaga ataacattcc attttattat gttcccaatt taattatatt
       61 aatatatatt ggcattaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca
      121 taactaacca tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag
      181 taagttggaa taaattgagt ttttgtgaca ttttactgaa aataataact ttcggcatat
      241 attctccaca cgaaactcta gccgaaaaac atagcgaaaa aaaactaatg gatagtttct
      301 caccatcact atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca
      361 tttcaatacg gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata
      421 aaatacaatc cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc
      481 ccaaacaatt caacttagga aaagtacata ctattacagc tccggtatca gggaatttta
      541 aaactcacaa gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa
      601 taccaaacga tgattacttc cacgttaaag acactgattt taatagtgtt tggcatgata
      661 tttacagaga tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg
      721 aaataccact aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg
      781 atagcaaaaa agagcttcaa atgctaagtt acaaccaagt aaataaaata atcaattcaa
      841 acttccccca acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc
      901 aagaccctgc atttatatct gcactaacat ctgcattctg gcaatctctt cacatcacgt
      961 cttcctctgt ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta
     1021 atttcatgcc tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag
     1081 ttgtccctaa aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct
     1141 catcaatatt accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa
     1201 gctatctcgt tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc
     1261 cagaaagtcg tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat
     1321 tggctgccag actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag
     1381 ctaaaaggat ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc
     1441 ccggaaatcc tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca
     1501 cgtttttccc ctgaagtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa
     1561 tatgactcac aatgggcagc gatttgttcc attgccccaa agattggctg tatactggaa
     1621 actctgcgtg tctgggtacg ccagcatgag cgggataccg ggggcggtga gg
//
LOCUS       DAEBXD010000301         1669 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEBXD010000301 DAEBXD010000000
VERSION     DAEBXD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.308"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(5..508)
                     /locus_tag="KJL94_004378"
     CDS             complement(5..508)
                     /locus_tag="KJL94_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBD4412434.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(553..1516)
                     /locus_tag="KJL94_004379"
                     /pseudo
     CDS             complement(553..1516)
                     /locus_tag="KJL94_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          473 a          362 c          338 g          496 t
ORIGIN      
        1 tccattaact ggcctcctgc gcaggttgtt tcagttgttg aatgctttga ttaataaaac
       61 acaaggcata aaacatcatt accactccac ttaatggcag aatgatatat acgtatccca
      121 tcggtatttg catagcggca gatacctgtg atgttgccag tgttttatca attaatttca
      181 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat gagaacgtta atgactatac
      241 taagaagtaa ttgtttgcgc ttattcaggg cgagagcaaa taaatcaata gagagatgtc
      301 gctgcgcgcc gacggtatat gcagcaccta ataatcctac ccagatcatc aaaaagcgtg
      361 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
      421 ctacgcatac aaccagagca agcattacgg cgacagaaaa cgcggcaatg acgcggtcta
      481 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagaggc
      541 tggcccggaa tattattgag cagcgttgtc gaatttctct aacaaagcag cttgttttgg
      601 atcttttttg aagtcgtcaa acagaggttg cacggcttta cggaatggcg ctttatccac
      661 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
      721 ccacaatttt tgttgataag cttcggattt cttcgccgct gtttcgagga tttgttgctg
      781 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
      841 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca tccaggaagg
      901 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
      961 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
     1021 ctgaacacga attttcagtc ctttaagatc ttctggcttt gtaatcggtt ttttcgcata
     1081 gaaactgcga gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
     1141 gtccataatt tctttaccaa cttcaccaaa aacaacttaa taagaacggc aaattataga
     1201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
     1261 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
     1381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgcc actttttctg
     1441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1501 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1561 tgtgcttatc agtgatttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1621 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcc
//
LOCUS       DAEBXD010000302         1663 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEBXD010000302 DAEBXD010000000
VERSION     DAEBXD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.309"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..204
                     /locus_tag="KJL94_004380"
                     /pseudo
     CDS             1..204
                     /locus_tag="KJL94_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            201..506
                     /locus_tag="KJL94_004381"
     CDS             201..506
                     /locus_tag="KJL94_004381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412435.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(740..1180)
                     /locus_tag="KJL94_004382"
     CDS             complement(740..1180)
                     /locus_tag="KJL94_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HBD4412436.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQ
                     DAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
     gene            complement(1152..>1663)
                     /locus_tag="KJL94_004383"
     CDS             complement(1152..>1663)
                     /locus_tag="KJL94_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412437.1"
                     /translation="CSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVK
                     TALENLGLGEAAKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPN
                     VSSTYGMRFPMTFSRVLAVTVTLNVTGASGQPTVSATNVQNTGFDITVSPGSGYGSSA
                     DAYYIAMGY"
BASE COUNT          480 a          359 c          318 g          506 t
ORIGIN      
        1 tattgtgctg tattggtaca tcaacaaaag caaagatatt attttatcta atcgcatttt
       61 tattttcgat cagttcaatt aaatcttaca caaataatgc ttcaccatta cacgatcagg
      121 agaagattgt taaatcatac attgattttt taaaaaaaaa taatctccat tatgggtacg
      181 gctctttttg ggatttgtca atgactgtaa attggttatc tggtggggac atacagataa
      241 ctcctgtttt ctttaatgct gattcgggga aaattaactt tactggcgtg agacaacaga
      301 cactggcttc ttggcatagc aaggaggcgt tcaattctgc tcctgagcga cagtttatag
      361 cagtttccat agctaacgaa cctgaacgat gtaaagaaat gacatcctgc cttgcaggta
      421 ttcaagaaca attaggtaag cctgacgaag ttttgaactt tgaaggaaga gtaatattag
      481 tatttaacaa aaaacttaac ctatagtaaa ttgcccccta ccatataaac cacactaacg
      541 aatgaataac ccgtcttttt cgacgggtta tatccctatc aggaataaaa atggagtggt
      601 tgccgtatac aacaaaccct catttagagc tatattctag agtatatgag atactataaa
      661 gttgaaggag ctatcattag aaaaaccata tgctgttata attagatgtc tcgcttttca
      721 tccaataacg aattttctct tatagattgc tctctggttt tttcggccac tctgtatttt
      781 ttatatcaac acgcatcaat agtgcgcgat attttttcca tgccagtaac agtttcgttt
      841 catcatctgt tgccatatct aaatcaaccg catcctgaag cggcgctatt acattcgttg
      901 cctctgctat cagtgcagat ttcttttcat tatttattaa ggctaattgt tctggtgttg
      961 gagaaggacg gtcaacaatg acagggtgac cattctcacc acaggtaatc atttttgtta
     1021 cactctgcga attaatcagt gttttccact ctgattccga gatttccact gcatcatcag
     1081 gaatattggt gccgtgaaat tcagtcgaat aaaatccgtt tgtcgatgca gaataaaaat
     1141 atgacatttc gttaatatcc cattgcaatg taatacgcat cagcagatga accatatcct
     1201 gaaccgggcg acactgtaat atcaaatcca gtgttctgga catttgtcgc cgatacagtc
     1261 ggctgccctg acgcgccagt aacgttcaat gtaactgtga cagcaaggac tcttgagaat
     1321 gtcatcggga acctcatccc gtatgttgaa gatacattag gggagccatt caactgaccg
     1381 cactgtaaaa ttaatccaga cggaaatttt tgcgcagcag ccgttgcggt attacttgac
     1441 gcgaacaggc tcatatcagg tatctgattc gcccctgtac ctacattcct tttagctgct
     1501 tctcccaaac caaggttttc gagagccgtt ttcaccgtgc catccgattt gatatcgcca
     1561 aacggattct tgcggcttaa cagcagcgcg cgaagtgcgg taagcaactg atcgtgccgc
     1621 cccttctcca ggctggcacc ggatgcctcc accacgctgc aaa
//
LOCUS       DAEBXD010000303         1605 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEBXD010000303 DAEBXD010000000
VERSION     DAEBXD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.310"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(404..478)
                     /locus_tag="KJL94_004384"
     tRNA            complement(404..478)
                     /locus_tag="KJL94_004384"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Gly,seq:tcc)
     gene            complement(482..557)
                     /locus_tag="KJL94_004385"
     tRNA            complement(482..557)
                     /locus_tag="KJL94_004385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Thr,seq:tgt)
     gene            complement(560..636)
                     /locus_tag="KJL94_004386"
     tRNA            complement(560..636)
                     /locus_tag="KJL94_004386"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Arg,seq:tct)
     gene            complement(638..713)
                     /locus_tag="KJL94_004387"
     tRNA            complement(638..713)
                     /locus_tag="KJL94_004387"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Ile,seq:cat)
     gene            complement(890..1579)
                     /locus_tag="KJL94_004388"
     CDS             complement(890..1579)
                     /locus_tag="KJL94_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD4412438.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          422 a          420 c          342 g          421 t
ORIGIN      
        1 tttttctaat cgttactggt gagaaggcgg aagaaaaagc cagttcttcg cccctaaata
       61 acagcatgat tccagtccat gcaaaatctg atttccagct cacacatacc gagcgctgta
      121 caaaaaaccg ccaggcaagg cagttaagag tgtgttcccg gggtttgctt agaatatttt
      181 gtaagttgtc cgaagagata tttacaacac cagaatgatg aatcatcagt cccctgccag
      241 aggttcacta cgtaacctga ccgacaatgc accattctgg tgctgaaaaa agagcactgg
      301 aactgcaaca aaacaacatc acagaacagg agatggtaag gagtcagaca ttcccacaca
      361 atattgtgtc agtgcaaata acaaccttcg tctcaaatct cgctggagcg ggcagcggga
      421 atcgaacccg catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat
      481 atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag ttgctctgcc
      541 tgctgagcta agtcggcgct ggcccaccac cgaggactcg aacctcggac cgtcaactta
      601 gaaggttgat gctctatccg gatgagctag tggtggttgg tggcccttgc tggacttgaa
      661 ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccggaggca
      721 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      781 cctgtgccat ttttcaggca taaaaaaacc gctcggcggc gggttttgct tactttacca
      841 tcgcgtacaa aatcggcaaa gtatcagatt tgcatgagat atatgccttt taatctactt
      901 ttgcaatact ttgctttgaa aatgccgctt tttgttctga acgtgttctc atcacaaaca
      961 ataaagcctc actatccagt cggtgaaaaa tgtgtttcat tacaacccag tgaccagtaa
     1021 atgttttgga ccagtttttg gttgttactc ccgccagtaa tgccagctcc tgatattcat
     1081 aaccttcccc accaaaaagt tctgctttta ccgcctgcgc cgccagccag attaattttt
     1141 tcaggcgttc ctgtgttttc tctgcaattt tcctagtgcc agaatgagcc ttaaactctt
     1201 cccacgccca gtgggttatg gtgatctgat actcccagca aatacttccg ctgtaacacc
     1261 acaacaacca ggctttatag tgctcttcaa gagacagaac agcccgccgc cacgatgatg
     1321 tcgaaaacgc aacctgactg accagaggaa ttgacgcccc cttcgccagt gattgttttc
     1381 ccgggattgg tggattatcc cgcgttatca tttttccggt cacctcatcg cggtaccgga
     1441 tttttttacg cctgtaacgc cctgtatcga acatggcatt ctcctgccag gcttcaagct
     1501 gaccttttgt cgccccactc aaatcagcgg tggcgataat gagctgctca cgaacaaact
     1561 gtaaatactg gttattcatg cacactccag ctctgtgatt tttat
//
LOCUS       DAEBXD010000304         1603 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEBXD010000304 DAEBXD010000000
VERSION     DAEBXD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.311"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(335..481)
                     /locus_tag="KJL94_004389"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="KJL94_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="KJL94_004390"
     CDS             535..717
                     /locus_tag="KJL94_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBD4412439.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="KJL94_004391"
     CDS             complement(818..1147)
                     /locus_tag="KJL94_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBD4412440.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="KJL94_004392"
     CDS             complement(1319..>1603)
                     /locus_tag="KJL94_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412441.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          437 a          368 c          376 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcagaaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAEBXD010000305         1598 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEBXD010000305 DAEBXD010000000
VERSION     DAEBXD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.312"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            154..477
                     /locus_tag="KJL94_004393"
     CDS             154..477
                     /locus_tag="KJL94_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBD4412442.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="KJL94_004394"
     CDS             474..890
                     /locus_tag="KJL94_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBD4412443.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="KJL94_004395"
     CDS             856..1380
                     /locus_tag="KJL94_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412444.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1598
                     /locus_tag="KJL94_004396"
     CDS             1377..>1598
                     /locus_tag="KJL94_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412445.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          389 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagc
//
LOCUS       DAEBXD010000306         1538 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEBXD010000306 DAEBXD010000000
VERSION     DAEBXD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.313"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(910..1386)
                     /locus_tag="KJL94_004397"
     CDS             complement(910..1386)
                     /locus_tag="KJL94_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4412446.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          346 a          410 c          386 g          396 t
ORIGIN      
        1 aaaaaaacaa cagcttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tcctggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAEBXD010000307         1478 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEBXD010000307 DAEBXD010000000
VERSION     DAEBXD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.314"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(38..583)
                     /locus_tag="KJL94_004398"
                     /pseudo
     CDS             complement(38..583)
                     /locus_tag="KJL94_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1238..>1478)
                     /locus_tag="KJL94_004399"
     CDS             complement(1238..>1478)
                     /locus_tag="KJL94_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412447.1"
                     /translation="GVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVV
                     DVLATGSVKVKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          429 a          313 c          312 g          424 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctggg ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg aacctaggta tcgagggtat
      841 cgaagataat cagccgctaa ccatttgttt ccttaaagga tgatgaatcc ttgtggttac
      901 agttatggtt acacattaat ttatctctgg tcaagtcccg aagtcctaac tttaagacag
      961 tggccccaat cgtccagact tgaaaggggc ctatggggga agtcgctgac ctttatctat
     1021 taaataccta cagagatttt tgtgagattt tttaatccaa ggtcataacc tactgccccc
     1081 acaggccgca aagtgaagac ctgtaagcac cagagcaata gcgagtatct atcactccct
     1141 gtagagtatc tggtagacca agaggacaca gaggacgacc atatacaact cctgtaagct
     1201 catggatgta ctccttatgt tcactcaggg tttatagtta ctctgcggca gtaaaacgaa
     1261 taacatggac caacgggaaa gaggcgtctt cctcaacgtc aaactcacct gctttcacct
     1321 taacggaacc agtagccaac acatcgacca cactgattaa ctcaaggtca tacccagcag
     1381 tgttgctatt tcgggtcact ttgacatcta ccgtcttagc gtctttagtt gacctgaaag
     1441 agatagaagc gaaccgctta ggcaggagat agacacct
//
LOCUS       DAEBXD010000308         1462 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEBXD010000308 DAEBXD010000000
VERSION     DAEBXD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.315"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            69..852
                     /locus_tag="KJL94_004400"
                     /pseudo
     CDS             69..852
                     /locus_tag="KJL94_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            859..1462
                     /locus_tag="KJL94_004401"
                     /pseudo
     CDS             859..1462
                     /locus_tag="KJL94_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          401 a          332 c          420 g          309 t
ORIGIN      
        1 atcgtgccgg attgttttgt tcggtatcag ttaaatgtaa cgctgcgagc gttacaaggt
       61 gaaaacaaat ggcttcaaac tggataaagc tcgaggttat tacgccggat aagccggaaa
      121 tattcaggct tgctgagatt ctgaatattg atccagatgc cgcattaggg aaggttattc
      181 gcttctgggc atgggcggat caacaaatga tagacggtaa tgcagattgt aacgctcgcg
      241 gcgttacaaa aagtgcaata gatcgcatca cttttatgtc tggttttgct gatgcgttaa
      301 ttcaggttgg atggctggtc gaaaatgacg gtgggctttc tctacctaac tttgaacgtc
      361 ataacggaaa aagctctaaa aaacgggcgg ttacaaacga gcgagtaaca aaaatacgcg
      421 aactgaaacg aaaaggtaac gctgccagcg ttacacaaac ggatcaaaaa gcgttaccag
      481 tggaagagga agaggaagag gaagatctaa atactgatct ccccctaaat ccccctcgcc
      541 aaaaacgagc gtctaaaaaa ttcgagccgg aggctattga gctgcccgat tggttgccgg
      601 aaacactctg gcatgagtgg gtccggttca gacaggcatt gcgaaaaccg attcgaacgg
      661 agcagggcga tacgggaact ggaaaaattc cgtcagcagg gttttacacc tgagcaggtg
      721 attcgacaca gcatcgccaa tgaataccag ggcctgttcg cgccgaaagg tgttcggcct
      781 gagacgttgc tccgacaggt taacaccgtc tcgttgccgg acagtgcgat cccgccaggc
      841 ttcagggggt aacggaccat gaaaaatatt gcgacaggcg gcgttctgga acgcatccgc
      901 cgactgaccc cgcaacatgt aaccgcccca ttcagaacgg tagcggagtg gcgcgagtgg
      961 caacttgctg aaggccagaa acgtagcgag gagatcaacc gcctgaatcg ccagttgcgg
     1021 gtggaaaaaa ttctgaatcg ctcaggcatc cagccgttgc accgtaaatg ctcgtttgcg
     1081 aattaccagg tgcagaacga cggccagcga tacgcgttaa gccaggcgaa atccatcgcc
     1141 gatgaactga tgaccgggtg tacaaatttt gcgttcagcg gaaaacctga taccgggtag
     1201 aatcacttag cggcagctat cgggaatcgc ctgctgaaag acggtcagac agtgattgtg
     1261 gttaccgtgg ctgatgttat gagcgccctg cacgccagct atgacgatgg gcagtcaggc
     1321 gaaaaatttt tgcgggagct gtgcgaagtg gatctgctgg ttcttgatga aattggcatt
     1381 cagcgcgaga cgaaaaacga gcaggtggtg ctgcaccaga ttgttgatcg ccggacagcg
     1441 tcgatgcgca gcgtggggat gc
//
LOCUS       DAEBXD010000309         1456 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEBXD010000309 DAEBXD010000000
VERSION     DAEBXD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.316"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            49..1038
                     /gene="ldhA"
                     /locus_tag="KJL94_004402"
     CDS             49..1038
                     /gene="ldhA"
                     /locus_tag="KJL94_004402"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD4412448.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1149..>1456
                     /gene="hslJ"
                     /locus_tag="KJL94_004403"
     CDS             1149..>1456
                     /gene="hslJ"
                     /locus_tag="KJL94_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBD4412449.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT          394 a          340 c          365 g          357 t
ORIGIN      
        1 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
       61 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
      121 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc
      181 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc acccggtgct ggaagagctg
      241 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
      301 gatgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaggcc
      361 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat
      421 cagcgtaccc gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc
      481 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
      541 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc
      601 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
      661 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
      721 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcggcaatt
      781 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtctatga gaacgaacgc
      841 gatctgttct ttgaagataa atccaacgac gtgatccagg atgacgtatt ccgtcgcctg
      901 tctgcctgcc acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagctctg
      961 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg tgaaacctgc
     1021 ccgaacgaac tggtttaatc ttgcagctcc cctgcaatcc aggggagctg attcagataa
     1081 tccccaatga cctttcattc tctattctta aaatagtcct gagtcagaaa ctgtaattga
     1141 gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
     1201 taagtaatga caaaattgct gttacgccag aacagctaca gcatcatcgc tttgtgctgg
     1261 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
     1321 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
     1381 atggtgaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
     1441 ttaatgaact cgataa
//
LOCUS       DAEBXD010000310         1427 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEBXD010000310 DAEBXD010000000
VERSION     DAEBXD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.317"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            663..947
                     /locus_tag="KJL94_004404"
     CDS             663..947
                     /locus_tag="KJL94_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBD4412450.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            947..1222
                     /locus_tag="KJL94_004405"
     CDS             947..1222
                     /locus_tag="KJL94_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD4412451.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          389 a          358 c          356 g          324 t
ORIGIN      
        1 gtgtgaaaat gatggcaaaa acagcagaca gtatcagtac gataaatgtg gccccccatc
       61 ccagataatc gacaacaagt cccattccca gtgtcgcaac aattgagccg aagatatagg
      121 aaaatgtgcc tgacagccct aacagaataa gtttacgcgt aaatcgcccg gaatccagaa
      181 gagcattaag tatcacataa acaggttcca taccagtcct gaccgcatca ggacgccagt
      241 aaataaaatc tcgtagtcca ccggcatcag agacaagagt aatcgtcgtt accggtattt
      301 cgactggttt gtactggtgt gagcggacaa aactactgta cgtctctcat acacgctcgt
      361 aagcggccct cacggcccgc taacgcggag atacgccccg actgcgggta aacccttgtc
      421 gggaccactc cgaccgcgca cagaggcatt ttcatcgctg acaggccgca tggcctcgca
      481 gagccggagg gggactggtg atattcggac gaccgtaccg gcttgcgccg ggcttcggcg
      541 gttttacacc tgtgctacat gtaaccaccg tatgccgcct tatatgcgct gactcgcatt
      601 atttgccttg ttccgtctga aagtaatcac tatgattaaa tatgattaac aactaatcgg
      661 gtatgcaaat gaaaaacaat accgcacaag caacaaaagt aattaccgcg catgtgccat
      721 tacctatggc tgataaagtc gaccagatgg ccgccagact ggaacgctcc cggggctggg
      781 ttatcaaaca ggcgctttct gcatggcttg cccaagagga ggagcgtaat cgcctgacgc
      841 tggaagccct ggacgatgtg acatccggac aggttatcga ccatcaggct gtacagtcct
      901 gggcggacag cctcagtact gaccatccgt taccggtgcc acgctgatgg aactgaagtg
      961 gaccagtaag gcactttctg atttggcgcg gttatatgat tttctggtgc tggccagtaa
     1021 acctgctgcc gccagaacgg tgcagtccct gacacaggcg ccggtcattc tgttaactca
     1081 tccacgtatg ggagaacagt tgtttcagtt tgaacccagg gaggtcagac ggatttttac
     1141 tggcgagtac gaaatccgtt acgaacttac tggccagact atttatgtat tgcgcctgtg
     1201 gcacacacga gagaacaggt agattgggtg tggaggcaac tttaacatag cctgcaggta
     1261 ttaattctgg agcattgtta tcaggccaga tattttgcat tccattaaat aatcttgtag
     1321 gagataaaat aaattacgat gaaataacga aaattaccgc tcgtgactgg cggcaatatc
     1381 gcgctccggg ctggcaaata acccatcaga aacgttattg ccagaca
//
LOCUS       DAEBXD010000311         1405 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEBXD010000311 DAEBXD010000000
VERSION     DAEBXD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.318"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..168
                     /locus_tag="KJL94_004406"
     CDS             <1..168
                     /locus_tag="KJL94_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBD4412452.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            153..791
                     /locus_tag="KJL94_004407"
     CDS             153..791
                     /locus_tag="KJL94_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBD4412453.1"
                     /translation="MGDSVTPETLGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..>1405
                     /locus_tag="KJL94_004408"
     CDS             1019..>1405
                     /locus_tag="KJL94_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412454.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          394 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgatttct ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ctctgtgaca ccggagacac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat gcaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa gatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagt
//
LOCUS       DAEBXD010000312         1402 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEBXD010000312 DAEBXD010000000
VERSION     DAEBXD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.319"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(418..1158)
                     /gene="phoN2"
                     /locus_tag="KJL94_004409"
     CDS             complement(418..1158)
                     /gene="phoN2"
                     /locus_tag="KJL94_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBD4412455.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          401 a          270 c          292 g          439 t
ORIGIN      
        1 attcaaaaat gattgatttt ttattaaaaa atggagcggt atcaggcaaa cgatttgaga
       61 ggtgacctaa aatatattta ggttgtgccc tgaatctgaa gttgtcattt ctgttatatt
      121 gatcgacata ggggatggtg cagaccgttc ccctgcaaca ccacattgca tgtggtgaac
      181 ggaaccgccc cgagtttcct agagaatgtt ttatctgtga actcaggttg ccagatcatc
      241 gtttccgatg gcagcataat aagctttttc tgcttctgcc ggagggatat gtcccagcct
      301 ttccagtaat cccttaaatt caggggtaaa acgtggtgtg cccatagact cctcctatgc
      361 tcatataggg tagatttgtc tatgggccct ccatagcctg atacaggctg tccagcttta
      421 tggggtcagt tcattggtag gagtatttaa ttcttcaaac tcttttttgg cttcgctaag
      481 gcttttggta aattcaggtg tattatgaag tactgcaaca accgatgctc ccattaaacg
      541 cccagcctct acatcgcttt gccaatgcgc accgcagatg acccgacttt ctccaaactc
      601 atatccacgt cgaagtattt ccgctttacg ttgaggatta atctccgcaa gtatcagtgc
      661 tactgcccaa ccaaaggatg catgaccaga gggataagag ccagtgatag ccattttctc
      721 atctttatca ggtgtacagg ttgcgtcttt ataaataacg aatggacgaa ctcttttata
      781 atattctttg gcgttacgca cggcgtaatc atggctatcc tgtagtactt gtgttaacaa
      841 ctgatataag attggggtat tttcccttga aatatccatg ccataagcat ctgaaaatgc
      901 aagaccaaaa ttctcgtagt atgcatcttc gctagcgaga cgtacacgat tagcatcccg
      961 gagcgagcgg ccgaattcat aatgagcttt gtcagcctga aatactactg aatcctctgc
     1021 cggaggcggc ggaagtattg acaaactgtc tggtgaagtt tgttgagtga gaaaaccttc
     1081 tgccttcaga gcatttgctg aggggataaa aatcatattt gtagcaatac aaaaaagaag
     1141 aaagtttttg gttttcataa tttatttcct tagcataatt aattgatgat tttggtcagc
     1201 tgtaaaaaac gagctgtatt acaaaaaaca caagtcatgc ggaaaaattt gaacagagtg
     1261 atggaaaaac aaaaaatagt cttatattaa agtaataaca ttatttttca tattcacgga
     1321 aaataatgtg gtaaaagaat gatgtttttt atctattttt tcgtatcgtt ttgtctctgt
     1381 attatgatga tttgtgattt tc
//
LOCUS       DAEBXD010000313         1395 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEBXD010000313 DAEBXD010000000
VERSION     DAEBXD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.320"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..695)
                     /locus_tag="KJL94_004410"
     CDS             complement(<1..695)
                     /locus_tag="KJL94_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4412456.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1395)
                     /locus_tag="KJL94_004411"
     CDS             complement(1011..>1395)
                     /locus_tag="KJL94_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBD4412457.1"
                     /translation="ESKIKHKISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKS
                     CVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHI
                     LSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          407 a          242 c          296 g          450 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggga ataataagct gaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcgg aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgtggctc cgtatagagt tccatcatca gttaaggcct gcgccgcatt gcgtattaca
     1261 cagctttttg tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa
     1321 tcaaattgac catgtaacgc cgcaggataa ggttcaaaaa catcatggct aattttatgt
     1381 ttaatttttg attcc
//
LOCUS       DAEBXD010000314         1356 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEBXD010000314 DAEBXD010000000
VERSION     DAEBXD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.321"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(41..100)
                     /locus_tag="KJL94_004412"
     CDS             complement(41..100)
                     /locus_tag="KJL94_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD4412458.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="KJL94_004413"
     CDS             complement(339..1247)
                     /locus_tag="KJL94_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412459.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            complement(1281..>1356)
                     /locus_tag="KJL94_004414"
     CDS             complement(1281..>1356)
                     /locus_tag="KJL94_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4412460.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAEBXD010000315         1353 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEBXD010000315 DAEBXD010000000
VERSION     DAEBXD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.322"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..226)
                     /locus_tag="KJL94_004415"
     CDS             complement(<1..226)
                     /locus_tag="KJL94_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412461.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKE"
     gene            303..845
                     /locus_tag="KJL94_004416"
     CDS             303..845
                     /locus_tag="KJL94_004416"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412462.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            1072..>1353
                     /locus_tag="KJL94_004417"
     CDS             1072..>1353
                     /locus_tag="KJL94_004417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBD4412463.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          373 a          285 c          329 g          366 t
ORIGIN      
        1 gctctttcag acgctgacgt tcagcggtgg tgagccctcc atcaccgccc ccggtatccc
       61 gctcatgctg gcgaacccag acacgcagag tctccggcgt acagccaatc tttggggcaa
      121 tggaacaaat tgtcgcccat tgtgagtcat attcgccctg actttccaga accatacgga
      181 ctgcccgttg acggacttcg ggggaaaaac gagtattttt agtcatcctg tttacctctt
      241 tctcaggaag tttagtctcc aggattcccg gggcggttca ggataacacc aaaggcgatg
      301 ccatgattgg cgtcaagcag cctttaccaa aagcagtttt aagaactcag catgacaaaa
      361 ataaagaagc aataagtatc ctggattttg gtgttattga tgatggtgtg acagataatt
      421 accaggcaat acaaaatgct atagatgccg ttgcttcact accctccggc ggggagctgt
      481 ttatccctgc gagcaaccaa gcggtggggt atattgttgg atccactttg cttattcctg
      541 gcggtgttaa catcagagga gttggtaagg catcgcaact ccgagcaaaa agcggactta
      601 caggatctgt gttaaggctg tcttatgatt cagacactat cggccgttat ctgagaaata
      661 tacgagtaac tggtaataac acctgcaatg gtattgacac aaacattaca gcagaagact
      721 ctgtcatcag acaggtttat ggctgggtat ttgataatgt aatggtgaat gaagttgaaa
      781 ccgcattatt taatgcaagg gctctggcac tcaaaattta tagcatgtca ggctggaacc
      841 tgtagagtcg gtcttcactt tttaggccag tgcgtaagtg ttagtgtcag ctcctgccat
      901 ttcagcagag gaaattattc tgctgatgaa agctttggca tcaggattca gcctcaaacg
      961 tatgcgtggt cgtcagaggc agttaggtca gaagcaataa ttttagacag tgagaccatg
     1021 tgcattggtt ttaaaaatgc cgtctatgtt catgattgcc ttgatttgca tatggaacaa
     1081 ctggatttag attattgcgg ctcaacaggc gtggttatag agaatgtaaa cggaggattt
     1141 tctttctcaa actcatggat agcagcagat gccgatggca ctgaacaatt tacggggata
     1201 tattttagaa cgccggcctc aacgcagtca cataaaattg tcagtggtgt tcatatcaac
     1261 actgcaaaca aaaacacggc tgcaaacaat caaagtatag cgatagaaca gtcggcgatc
     1321 ttcgtctttg taagtggttg tacgttaact ggt
//
LOCUS       DAEBXD010000316         1331 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEBXD010000316 DAEBXD010000000
VERSION     DAEBXD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.323"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            29..910
                     /gene="yddG"
                     /locus_tag="KJL94_004418"
     CDS             29..910
                     /gene="yddG"
                     /locus_tag="KJL94_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBD4412464.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1165..>1331
                     /locus_tag="KJL94_004419"
     CDS             1165..>1331
                     /locus_tag="KJL94_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD4412465.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKT"
BASE COUNT          341 a          283 c          308 g          399 t
ORIGIN      
        1 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
       61 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
      121 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
      181 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
      241 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
      301 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
      361 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
      421 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
      481 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
      541 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
      601 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
      661 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac
      721 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
      781 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc
      841 gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
      901 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
      961 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     1021 acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt
     1081 aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa
     1141 cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt
     1201 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
     1261 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
     1321 gataaaactt a
//
LOCUS       DAEBXD010000317         1328 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEBXD010000317 DAEBXD010000000
VERSION     DAEBXD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.324"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(3..485)
                     /locus_tag="KJL94_004420"
     CDS             complement(3..485)
                     /locus_tag="KJL94_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4412466.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="KJL94_004421"
     CDS             complement(476..778)
                     /locus_tag="KJL94_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD4412467.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="KJL94_004422"
     CDS             1050..1322
                     /locus_tag="KJL94_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412468.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          392 a          330 c          282 g          324 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttca
//
LOCUS       DAEBXD010000318         1322 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEBXD010000318 DAEBXD010000000
VERSION     DAEBXD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.325"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            88..153
                     /locus_tag="KJL94_004423"
                     /pseudo
     CDS             88..153
                     /locus_tag="KJL94_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="KJL94_004424"
     CDS             381..629
                     /locus_tag="KJL94_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD4412469.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="KJL94_004425"
     CDS             complement(745..1014)
                     /locus_tag="KJL94_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD4412470.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..>1322
                     /locus_tag="KJL94_004426"
     CDS             1071..>1322
                     /locus_tag="KJL94_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBD4412471.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNE"
BASE COUNT          385 a          291 c          286 g          360 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aa
//
LOCUS       DAEBXD010000319         1304 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEBXD010000319 DAEBXD010000000
VERSION     DAEBXD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.326"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..636)
                     /locus_tag="KJL94_004427"
     CDS             complement(<1..636)
                     /locus_tag="KJL94_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD4412472.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1289)
                     /locus_tag="KJL94_004428"
     CDS             complement(750..1289)
                     /locus_tag="KJL94_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4412473.1"
                     /translation="MASLMLAGHALAEDGVVNFVGKIVDTTCEVTSDTADQIVPLGKV
                     SKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGTPLTAGNP
                     GDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIARYVATNA
                     TVTAGTANADSQFTVEYKK"
BASE COUNT          352 a          303 c          282 g          367 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcc
//
LOCUS       DAEBXD010000320         1284 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEBXD010000320 DAEBXD010000000
VERSION     DAEBXD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.327"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(80..346)
                     /gene="mobC"
                     /locus_tag="KJL94_004429"
     CDS             complement(80..346)
                     /gene="mobC"
                     /locus_tag="KJL94_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBD4412474.1"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
     gene            complement(567..1040)
                     /locus_tag="KJL94_004430"
     CDS             complement(567..1040)
                     /locus_tag="KJL94_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBD4412475.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          313 a          337 c          288 g          346 t
ORIGIN      
        1 cacgagtatt gagcatagtc gagattggtg cagatcactt ctgatattga actgtcagga
       61 gctggctgca caacagccat tacgcccaat caactggtgc agtcgtcttc tgaaaatgac
      121 aatcgtgcca ccggctccag gtcttatcga acagctcaga cacatggtcc aaatgctggt
      181 cgatccgttc ggccagtttt tcgatggcct cttgcttgag cttgtcactt tccctgaact
      241 cgatgttcaa ggccctggcc acctggttga ggttccggcc gatagctcca agctgccggc
      301 acgattcccg tacggtgttc acttcttcat cagtgaggac cggcatccgg ttcaacgacc
      361 gcagtcattt ctgtacctaa acagtcctgc gaatatgtga gttttgacct aatgagccac
      421 aaaaactcat tttcgcccac gataattttt ctacttcctt tctttggaat gctcatttca
      481 atattcacat aacgtcgcta tcaatcgcgg aaaaggcgta gtgcgcgaag cgaacgaagc
      541 tttttgccgt cgattgcata gctttgttaa ccctttttcc aaatttgata gcaatagtta
      601 atgtttgaag taaagtgttg ctcaaaaaca acttcgaagg tatttggaat actcgggaag
      661 aaaacatccc cctctggctc gatgtcgatc gtcgataagt ggagcgtaga ggccatgggt
      721 aatgtttctc ggtaaatttc tccgccacca gacactataa cgtgaccggt gaattcagct
      781 agcctgtcca tggcctcttc gattgactga aatacaacta cattgtcatc atttgatgtc
      841 caacctgagc gggtaacgac cgcgtatttc ctattgggga gtgcgcccat agattcaaac
      901 gtcttgcgac ccaccagaag ccactgattg taggtcaatg ctttaaaaag tagctgctcc
      961 cctttcgcgg accagggtat gtctggaccg caaccaatca cgccgttttt cgctttcgca
     1021 gccatcaatg atactttcaa ggttctcatc ctgggttaac tttgttttag ggcgactgcc
     1081 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1141 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1201 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1261 gcgcatacgc tacttgcatt acag
//
LOCUS       DAEBXD010000321         1280 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEBXD010000321 DAEBXD010000000
VERSION     DAEBXD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.328"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..815)
                     /locus_tag="KJL94_004431"
     CDS             complement(<1..815)
                     /locus_tag="KJL94_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4412476.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="KJL94_004432"
                     /pseudo
     CDS             907..993
                     /locus_tag="KJL94_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          360 a          215 c          334 g          371 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact
//
LOCUS       DAEBXD010000322         1274 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEBXD010000322 DAEBXD010000000
VERSION     DAEBXD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.329"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(169..598)
                     /locus_tag="KJL94_004433"
                     /pseudo
     CDS             complement(169..598)
                     /locus_tag="KJL94_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(646..831)
                     /locus_tag="KJL94_004434"
     CDS             complement(646..831)
                     /locus_tag="KJL94_004434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412477.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            994..>1274
                     /locus_tag="KJL94_004435"
     CDS             994..>1274
                     /locus_tag="KJL94_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD4412478.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT          304 a          390 c          263 g          317 t
ORIGIN      
        1 ccaaaagatt aaaaaagcac cgaagaatgc gcgtttattc aacacaacct gatcatcaag
       61 taatcggtat agacacttaa tattgcttac attgattgat tcaatgtatc tctgatggtt
      121 ttttgctccg ttcccctcaa catggacgca acttcccctc tgcgactctc agccgcacca
      181 ccgtattcga gccagcggct catgcatcat aacctgctct gccagacggt aacccctctt
      241 cagccccgta aaccgcatct gacctccgcg cagctcgcac ttcagcgggt caaccttcag
      301 tcactgctga tacatccctc tccttgtgat ttgcctcgcc gtttttctca ctgtctccgt
      361 tatggcgtac accacatctt ccagtaatcg ccgcttcacc ggactcagga ccccgtatca
      421 ccatccggaa ccccttatcc gccacatgcc aggagaacct ttccataaac tcgtcgccac
      481 tcatcacaag gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg ccaatttcat
      541 cctgaccggc ataatgttcc aggcggctca tctgcaccgg ctgcttctcc agccagttaa
      601 gccatcggtt tccctgctga aaaatatcgg caaaacgggg aagcatcaga aaggcggaat
      661 aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt cggcgttgat
      721 accggcagga attgtcgcaa attcccgctc catgcagcgg gcaatttccg gatacccttc
      781 gtcatcaaca cgtacaaacc agaagaccag ttttttgttt ccggcatcca caaagaacgg
      841 aatattcagg tctgcgcaac actcaacggc atcgtcaaaa ttatcaaagc gcagaacctc
      901 agcttcttcg taaccggctc atttaaaccg tctggtctgt ttcctccggc tccacaaaaa
      961 taatgtccat cattttgaag tggacactat cgtatggaat accggacctg gatcactgaa
     1021 gctttacgcc ttcatttcga agaacattta ccacgggttg tagccggacg tcgcctgggc
     1081 gtaccaaaat caactgtctg tggtatgttc gtgcgcttcc gcaacgctgg cctttcatgg
     1141 cctctgcccg caggtatgtc ggagcaggaa cttgatgccc ttctttacgg aagtgcctcc
     1201 acagtacctg tcgtacttac tgaaagcacc gttatgccca aactcccggt ggtaaaaaaa
     1261 cgtccccggc ggcc
//
LOCUS       DAEBXD010000323         1253 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEBXD010000323 DAEBXD010000000
VERSION     DAEBXD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.330"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..809)
                     /gene="ipaH4.5"
                     /locus_tag="KJL94_004436"
     CDS             complement(<1..809)
                     /gene="ipaH4.5"
                     /locus_tag="KJL94_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBD4412479.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLT"
BASE COUNT          356 a          224 c          272 g          401 t
ORIGIN      
        1 gttaatcttt gcagggattg cagaacgtga gtcgacaatg gattccctga aatgttaaca
       61 ttcgtacaaa tgggcaatgt gattatattt tcgggtaatg aagttagtag gttattggaa
      121 acatccagtg ccaatagttc ttgcggtaat ctggataatc tgcgtagttg attttcacct
      181 accttcaaat attttaagct ctgaggaaat tctggtagtt cctgtagtct gttgccggaa
      241 acaaagagtt cttgtagtct atgaggcaat tcaggcaaga cagtaaggcg attaccttca
      301 atacgtaata ttgttagcgt tgatggaagc tctggtaacg tctccagact attaaaacgt
      361 gccgagagtg attgtaaata tggtggtaat gatggcagac atgacaaatt tcgattgaat
      421 gaaatattaa gcttaatcag ttgagatgga agtgtcggga gtatattcaa gttgttacca
      481 tttacatgaa gttctgtcaa aagcggagaa ttttcaggta gggagattaa ctcattgttg
      541 gaaatattaa gttcacgtat atgcaaaggg agtggtggta aagaacgtag tttcagtaag
      601 cttaaattga gaacaggctc ccgggtatcc agacatattt tcaataatcg aaccgcctgg
      661 atgcgatttt ctgttgttgt tccttcccta gcccagtcat cccagatgcg gtggtaatct
      721 ctggtacact gttcttctac cgataaacga gatatacatg ataagccaca aagggaacga
      781 aaaaaagaat gattgttgat cggtttcatt tattaacccc tcactattca tatcttggtc
      841 tattaatgca aataaaacga gaaagtcttt aaaaaaacaa tcctagaact ttatcggcgt
      901 tttcattccg gctggaggaa tatgttcctg tctttccagt aaccgtcgat tgttatatca
      961 gtctacccat gttaatgtga ccagttctac tttcatccag tttggattga tctttatccc
     1021 ctatattttc aaacaatttt tatcatttaa ctcattactg tctcgatatt cccatagcaa
     1081 acgatctggt tggttatccc ctcaaaaaag tcagttgcac gccgtaaatc cctgccggta
     1141 ttgagcgagg atttacgggt tgttgttaag gtccgtaatc gggtcacagt ttttccggag
     1201 tcaatccggt ctgcggttta tgcttatgcg acgtgattat gaatggtgca gtc
//
LOCUS       DAEBXD010000324         1221 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEBXD010000324 DAEBXD010000000
VERSION     DAEBXD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.331"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..348
                     /locus_tag="KJL94_004437"
                     /pseudo
     CDS             1..348
                     /locus_tag="KJL94_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            276..749
                     /locus_tag="KJL94_004438"
                     /pseudo
     CDS             276..749
                     /locus_tag="KJL94_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          302 a          301 c          369 g          249 t
ORIGIN      
        1 tactccgggt ggcccgcagc ggctggtata cgtggtgtca gcggcggaca aggataaaca
       61 cacgtcagca gttccgtcag cactgcgaca gcgttgtccg caaggctttt acccagtcca
      121 gcgttacggt gccccacgcc tgacggatga actgtgtgct ctggttacca ctttaacgta
      181 aaaaccgtgg cagccagtct gcgccgtcag gaactgagcg caaaagcctc ccagaagttc
      241 agcccgatca gttaccgtgc acacggcctg cctgtatcag aaaatctgct gacgcaggat
      301 ttttacgcca gtggcccgaa ccagaagtgg gcgggagaca tcacgtacta ttacagctca
      361 ccgactgcgg ggaaacacgg ggcgccgggt tattgaaggt gacctgtcca gctacttcga
      421 cacagtacat caccggctgc tgatgaaagc cgtctgccgc aggatcagtg atacacgttt
      481 tatgagactg ctgtggaaaa cgccgtgttg atgttggtct cttccgggcg gtcagtgaaa
      541 gtatgccaca gggcggtgtg atctcgccgt tgctgtcgaa catcatactg aatgagttcg
      601 accagtatct gaataaacgc tacctgagtg gaaaagccag aaaggatcgc tggtactgga
      661 atcacagtat ccagcgaggc cgaagtactg ctgtcaaaga aaactggcaa tggaaacctg
      721 ccgttgctta ctgttgctat gccgatgact gtgtgccacg aaggcgcgtc ctggggacgt
      781 gagtccctgt gcagccatgc agcacaagag atgagggaag gccccttggt gtcggcgtgc
      841 aggcgcaggc accaaaccat ccactgctgc tgcatttaag agatgtggtg gtgagcgaga
      901 tgggaaaagg tcggacgtga gccgatcagg tatggttcga agagacgaac gaaagtgaac
      961 cgctgatgat gtgtcgaaag catatagatg atgtcaaaac aatggtggag tcgttcctgt
     1021 gggatatgtt tactgcctgc gcggcatccg gcataaaagc ggcgtgaagt cgattcaggc
     1081 tcttgtgggg aacgtggacc tgtcaccccg atgtgaaggg agaagcgcaa gtggaagccc
     1141 catgagcgcc agagtaccaa tgcggggtac agggacggag caccaagcga ttgcgcagta
     1201 aacatgcttg acaacttaat t
//
LOCUS       DAEBXD010000325         1160 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEBXD010000325 DAEBXD010000000
VERSION     DAEBXD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.332"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            115..1160
                     /gene="cadB"
                     /locus_tag="KJL94_004439"
                     /pseudo
     CDS             115..1160
                     /gene="cadB"
                     /locus_tag="KJL94_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          247 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcagctatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctccta gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAEBXD010000326         1136 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEBXD010000326 DAEBXD010000000
VERSION     DAEBXD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.333"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            205..537
                     /gene="hdeA"
                     /locus_tag="KJL94_004440"
     CDS             205..537
                     /gene="hdeA"
                     /locus_tag="KJL94_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBD4412480.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            653..979
                     /gene="hdeB"
                     /locus_tag="KJL94_004441"
     CDS             653..979
                     /gene="hdeB"
                     /locus_tag="KJL94_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBD4412481.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1043..>1136
                     /locus_tag="KJL94_004442"
     CDS             1043..>1136
                     /locus_tag="KJL94_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBD4412482.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT          361 a          215 c          237 g          323 t
ORIGIN      
        1 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
       61 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
      121 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
      181 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
      241 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
      301 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
      361 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
      421 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
      481 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
      541 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
      601 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat
      661 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
      721 cgcacaatct gcgctggcag ccaatgaatc tgctaaagat atgacctgcc aggaatttat
      781 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
      841 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
      901 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
      961 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     1021 ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta tactcgcggc
     1081 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggt
//
LOCUS       DAEBXD010000327         1124 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEBXD010000327 DAEBXD010000000
VERSION     DAEBXD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.334"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            65..499
                     /locus_tag="KJL94_004443"
     CDS             65..499
                     /locus_tag="KJL94_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBD4412483.1"
                     /translation="MYGTCETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            550..>1124
                     /locus_tag="KJL94_004444"
     CDS             550..>1124
                     /locus_tag="KJL94_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412484.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGA"
BASE COUNT          251 a          318 c          348 g          207 t
ORIGIN      
        1 atggaggcgt ataccgaaat tctggaactg gattcctgct tcaaagattg agaaggagac
       61 gagaatgtac ggaacatgcg aaacgctatg ccgggagctg gcagcaaagt atccgggaga
      121 cgcgccgctg atgctggtta tctggtcccc ggaagagatt caggcactgg ctgacggtat
      181 ggaaatttct ctgaccgatc atgaaatcag gaccgtcctg gcgcgcatgg aggacatccc
      241 ggaagaccag cggactgaat ccggtatttc ttccgccgcg gtgatggaga ttatcagcaa
      301 cgtgagcgaa aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc tgattcagac
      361 cgctgaacag gcactgtgga aacgtgagtg ggccgcccgt gataacggcc tcgccgtccc
      421 ggaatgcgtt acccgccgtc aggcggtggt taatcaggcc cgcaccctgc tgaaaaacaa
      481 cacacacgaa aacaactgat gttatcgccg cctgccctgc gggcggcgat acagggagaa
      541 cgcttaatca tgaacgaaaa cagcacgctg aacgcactga tttgtcgtca cgcccgtaac
      601 ctgctgctgg cgcagggctg gccggaagag acggatgttg accagcgaaa tccgaactat
      661 ccgggctgga tcagcattta cgtgcggctg gatgcgcccc ggctggcgac gttacttatc
      721 aaccgtcacg gcggcgtact gccgccgctc ctggcctccg ccattcagag actgaccgga
      781 accggggcgg aactggtact gtccggcagt cagtggcagt cgctgccggt acttccggca
      841 gacggaacgc aggtgtcttt cccgtatgcc ggagaatggc tgaccgaaga cgaaatcagg
      901 gctgttcttg atgcggtgca cgatgcggta cgaagcatct gttaccaggt ggcagaagat
      961 gcgcggcgta tccgtgcggc gctgaccacc accggtcaga cgttgctgac ccgccagacg
     1021 cgccgctttc gcctggtcgt gaaggaaagc gatcacccct gctggctcga tgaagatgac
     1081 gaaaacctgc ccgtggtgct cgatgccatc gtgaaccggg gagc
//
LOCUS       DAEBXD010000328         1119 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEBXD010000328 DAEBXD010000000
VERSION     DAEBXD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.335"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            224..408
                     /locus_tag="KJL94_004445"
                     /pseudo
     CDS             224..408
                     /locus_tag="KJL94_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            551..772
                     /locus_tag="KJL94_004446"
                     /pseudo
     CDS             551..772
                     /locus_tag="KJL94_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(793..855)
                     /locus_tag="KJL94_004447"
                     /pseudo
     CDS             complement(793..855)
                     /locus_tag="KJL94_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          350 a          251 c          245 g          273 t
ORIGIN      
        1 ttcaatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct tcgtgaagct
       61 tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta cgggatttag
      121 ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta taacggtaat
      181 tgcagaccat gacaaaccgc agccgtcagg ctgcctactc gagtcacaag gctctttgtg
      241 tccgatatgc cggcagcgca ttacccatga aactggctgg aacgtacacc ataaggtgaa
      301 gaaagttatg ggggcggtga agaattatct aaccttgtct tgttacatcc aaattgccat
      361 cgacagttac acagttgtga agccggctct ctcaaaaagg gcttataaag gcttgagcgg
      421 tgtaccggga aaccggtatg caccgttctt aggggagggc agtccagcaa tgaactgtcc
      481 ttacccgaca aacatccaaa acgaacggaa cgtgctggaa tccgcatata acccgttgta
      541 aagtgaggaa atggacacct cacttgctca tgagaacgcc cgcctgcggg cactgttgca
      601 gacgcaacag gacactatcc accagatggc tgaatacaac cgcctgctct cacagcggat
      661 ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg gttgcgaaac tgcagcgtat
      721 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga ttccattttc
      781 acgggcgatc tacgccacac aggctctggg ttgcagtgac tgctccatta aggcgatttt
      841 gaactcataa gggaagtaac aaaaaaactg caaaagtaca ataattatat atggccaaac
      901 taacctctat taaaaaacat cagtttttca cacttagttg agcttcatga aaaaatcatc
      961 gaggatttca ttgcagaata cacattccac taactggaag cactccataa gaggaatcag
     1021 agaaagtatc gtttttttat agtaaaattc attgctgttc aataaaattg atatagttat
     1081 taaattatga aaataatttc acaaaaaaga ttaacttga
//
LOCUS       DAEBXD010000329         1110 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEBXD010000329 DAEBXD010000000
VERSION     DAEBXD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.336"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..1019
                     /locus_tag="KJL94_004448"
                     /pseudo
     CDS             1..1019
                     /locus_tag="KJL94_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAEBXD010000330         1096 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEBXD010000330 DAEBXD010000000
VERSION     DAEBXD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.337"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..104)
                     /locus_tag="KJL94_004449"
     CDS             complement(<1..104)
                     /locus_tag="KJL94_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBD4412485.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="KJL94_004450"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="KJL94_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBD4412486.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT          309 a          248 c          188 g          351 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tgtataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttct
//
LOCUS       DAEBXD010000331         1095 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEBXD010000331 DAEBXD010000000
VERSION     DAEBXD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.338"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            482..>1095
                     /gene="uacT"
                     /locus_tag="KJL94_004451"
     CDS             482..>1095
                     /gene="uacT"
                     /locus_tag="KJL94_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBD4412487.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKG"
BASE COUNT          263 a          253 c          236 g          343 t
ORIGIN      
        1 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
       61 tttaatgagt tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc
      121 atgaccgttt taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc
      181 gctttcatta tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat
      241 gcgagagtgc gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa
      301 ttccattgtc aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc
      361 gcaattcctg tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt
      421 agacagtttc attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact
      481 gatgagcgcc atagattccc aacttccctc atcttctggg caagaccgcc caactgatga
      541 ggttgaccgc atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt
      601 catgtacgca ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa
      661 agatgctatt gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt
      721 gcaatgtatc ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac
      781 ctttgctgct gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg
      841 gatatttggt gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg
      901 tcgcttgatg cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct
      961 tagcatcatt caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg
     1021 taatcccgtt tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg
     1081 ctatgcgaaa ggatt
//
LOCUS       DAEBXD010000332         1080 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEBXD010000332 DAEBXD010000000
VERSION     DAEBXD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.339"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..307
                     /locus_tag="KJL94_004452"
     CDS             <1..307
                     /locus_tag="KJL94_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412488.1"
                     /translation="LNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVG
                     IWCFAFTACACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(308..952)
                     /locus_tag="KJL94_004453"
     CDS             complement(308..952)
                     /locus_tag="KJL94_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4412489.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          278 a          247 c          256 g          299 t
ORIGIN      
        1 tcttaactcg gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt
       61 cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc
      121 aatgaccgtc gggatttggt gttttgcctt taccgcctgt gcctgtttga cggggatctt
      181 cccgaaaatg gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac
      241 gccgtttgtg ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag
      301 caaataatta ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat
      361 cgagaaatgc gcgcaagccg caggacatat actggcggtt tgggtaatag acttggaagc
      421 ctggacgctc tgtgatccag tcttccagta cacattctaa acgaccagta tcaaggtact
      481 ccttgatctg ttcatagagc agatacccga taccagcacc catcaaaacg gcctccagtt
      541 cagcatcaac atcatccaga atgatatttc ctgcgacagc aatttccagc tcctttgcaa
      601 attgccagtg gaaaggtttg ccgctgggat aacgaaaaac aacacattga tgattaagca
      661 gatcatgagg atggcggggt ttgccatatc ttgcaaagta ctctggcatt gctgctacac
      721 ataatttcac tggtgggccg atcgccacgc tgatcatatc tttttcgacg atgccactaa
      781 gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat aagagaatcg tcagttgtga
      841 gctctacttt aatatcagga tattcacgtg tgaaaccaac cagtaaaggc attaaaaata
      901 tacgtgcagc tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc agacgaaaat
      961 cattcatctc atctaacatg atctgaattt cgtcgaaagc tgggcgcaat cgttcataga
     1021 gattagaacc cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat aagcgaattt
//
LOCUS       DAEBXD010000333         1052 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEBXD010000333 DAEBXD010000000
VERSION     DAEBXD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.340"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..307)
                     /gene="wzy"
                     /locus_tag="KJL94_004454"
     CDS             complement(<1..307)
                     /gene="wzy"
                     /locus_tag="KJL94_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBD4412490.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     "
     gene            complement(327..>1052)
                     /gene="rfbG"
                     /locus_tag="KJL94_004455"
     CDS             complement(327..>1052)
                     /gene="rfbG"
                     /locus_tag="KJL94_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBD4412491.1"
                     /translation="YPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDI
                     WYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCT
                     YVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHEN
                     NQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALS
                     FWKLRRKKADKIYILLMLILNIF"
BASE COUNT          380 a          187 c          127 g          358 t
ORIGIN      
        1 atataagtga cagcataaaa aggtatggtt gcacatcaaa cagtatatca tttattttat
       61 taccagctaa taagccgaca aagaagccat aaaaaggaaa taaaagcaac catataaaaa
      121 gactgacaca tttttgggtg attgaaaaat tgaatgtaac taaattaaaa attaaaagta
      181 taaaactaaa tacaaataat aaatatcgca aagggaatat tcccaatggc tccagtaatc
      241 ttccaccacc accaataatc agttcaatac ataaaaatgt tataaaaatt ttatttatat
      301 tattcatatt cgtaaggtga tgttttttaa aatatgttta aaatcaacat taataaaata
      361 taaattttat cagccttttt ccgccttaac ttccaaaaac ttaaagccaa gatcaaattg
      421 cctatataac ctttacccaa ttgattattc acaaagctat catcagctaa ggcattagct
      481 attttagtta cttcttttcg ataccacttg tttctcacca aaccaagccg tttgtatttt
      541 gctttaaaac taatgtttgc tcccacttgg ttgttttcat gctgtctgta cagcattgtt
      601 ggttttctat caatatacca cgaataattt ctcgtccgcg caaatgaata taaaaaccaa
      661 tcatgcagac agatatcttg tgcagcattt ttattattta tgatgaactt cttaaattca
      721 ataagcgttt ctttttttat aacatatgta caccccggac ctgctgcttc aaaaaaataa
      781 tcgaattgag tttgcgactg agccttatca actaaatttt ttcgccctga tggataatat
      841 gcaattacat tgctggaata acaattcgca ttattaaata ccaagcagtc aattgcattt
      901 tttattttat ctttatacca aatatcgtcc tgatcactaa aacagatata atcatagttt
      961 tcaagatcga catctttaat taatctataa aaatttttac ctgccccccc aaacttattg
     1021 acgcttggaa gtatatttat aattgaggga ta
//
LOCUS       DAEBXD010000334         1041 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEBXD010000334 DAEBXD010000000
VERSION     DAEBXD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.341"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..769
                     /locus_tag="KJL94_004456"
     CDS             <1..769
                     /locus_tag="KJL94_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4412492.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1041
                     /locus_tag="KJL94_004457"
     CDS             790..>1041
                     /locus_tag="KJL94_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBD4412493.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
BASE COUNT          337 a          232 c          234 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag c
//
LOCUS       DAEBXD010000335         1036 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEBXD010000335 DAEBXD010000000
VERSION     DAEBXD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.342"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..371
                     /locus_tag="KJL94_004458"
     CDS             <1..371
                     /locus_tag="KJL94_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412494.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGTWYNVASI"
     gene            374..918
                     /locus_tag="KJL94_004459"
                     /pseudo
     CDS             374..918
                     /locus_tag="KJL94_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            942..>1036
                     /locus_tag="KJL94_004460"
     CDS             942..>1036
                     /locus_tag="KJL94_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD4412495.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTP"
BASE COUNT          294 a          229 c          263 g          250 t
ORIGIN      
        1 atccatccag gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc
       61 agagaaaccc ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt
      121 acggaaactt cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg
      181 gccatgaaat gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt
      241 cgggacacgg gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg
      301 ttgacggtgt gtattaccga ccactgcaaa aactgattaa tggcacctgg tataacgtag
      361 cgagtattta acaatgttgc atttaaaaaa tattactacg ggtaatccga aaacagcaga
      421 acaatatcag atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa
      481 ctggtatgaa gagctgaaga atttcgccag gacacaatta aaataactta caccggagac
      541 ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
      601 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
      661 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
      721 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgttgct ggggctaatc
      781 agcgacgaag accgggaaaa actgaaagcg taccgcattt acgcgaaatc gctgcaggcg
      841 atggatttca gtgtcatcac tgataaaacc tcatacaacg ccattgaatg gcccgcttct
      901 ccggaaattt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
      961 gtgcgctgac tgatggcgca ggtatctcca tgtccggata ccatattatt ctgaaatccc
     1021 gggtaaacac cccgga
//
LOCUS       DAEBXD010000336         1031 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEBXD010000336 DAEBXD010000000
VERSION     DAEBXD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.343"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>1031
                     /locus_tag="KJL94_004461"
     CDS             <1..>1031
                     /locus_tag="KJL94_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4412496.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAE"
BASE COUNT          319 a          193 c          269 g          250 t
ORIGIN      
        1 tgtctataac cacgaaccgt tatgcatcag gttatgccac actgactgaa gcagtaagtg
       61 cacaggatga acggaacaga aaaagagata agaatagtca cgatggctct acgatatcct
      121 tatcccagcc ccttggtaat atcgggaatt tgaacttcaa taccacgagg tataattctt
      181 cccggggcac aggaaacacg cggtctacca gcctgagtta tagtacggta tggagaggca
      241 taacattctc cattaactgg gcaaaaaacg acttgctgac atcgcataaa tggaaagtag
      301 atcggaaatt aagcgtgggt atttcagtgc cattatcgct gggtgacgaa aatcaaattt
      361 atgcgtcttc acagatgagt cgttcgggag agcagggtaa taactatcag gtttcattat
      421 caggtcagaa tagtggtggt gtatggtggg atgtagcgac aaatataact aacgctcatc
      481 agtcacaacc gaagagcacg atgaacattg tgcaagttgg caaaaatgga tcatatggtc
      541 agtttagtag ccattacagc tccagcgaaa acatgaaaca gctaggtgca aatttaagcg
      601 gtgggatact tattacaagg gatggactta catttggtca aaatgtggat gggacattag
      661 cccttataga ggcacccgga gcaacggggg ttaacgttaa tggctggccg ggactgagta
      721 ctgattttcg tgggtatgca atactgccag tgcagccata ccgaagagac gatgttattc
      781 tggatgaaaa aacgataggt aagaattacg acttaccaca aactagtcaa ttggttgtac
      841 cgacagctgg agccgttgtg cctgcgacat tggcagtgaa gagcggggat aaaggattag
      901 taaccttaaa acagaaagag ggtaaacccg tccccttcgg cgctgtgatt agttatagca
      961 aagacactga aaatatggcc ggcattgtag gtgaagatgg tattgcttat gtttctggct
     1021 tgagtgcgga a
//
LOCUS       DAEBXD010000337         1026 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEBXD010000337 DAEBXD010000000
VERSION     DAEBXD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.344"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(114..>1026)
                     /gene="ospC2"
                     /locus_tag="KJL94_004462"
     CDS             complement(114..>1026)
                     /gene="ospC2"
                     /locus_tag="KJL94_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBD4412497.1"
                     /translation="ANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYG
                     YMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRL
                     GIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKI
                     RLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSKFNFTKQD
                     VAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKANSGDTMLD
                     NAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          325 a          198 c          146 g          357 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga catcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagct
//
LOCUS       DAEBXD010000338         1004 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEBXD010000338 DAEBXD010000000
VERSION     DAEBXD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.346"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            195..911
                     /locus_tag="KJL94_004463"
     CDS             195..911
                     /locus_tag="KJL94_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4412498.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          345 a          162 c          189 g          308 t
ORIGIN      
        1 tatgctttca aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta
       61 aatataagat tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat
      121 gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg
      181 aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag
      241 acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac
      301 aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc
      361 aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag
      421 atgaaacgtt cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg
      481 ctgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata
      541 aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg
      601 tgattgtatt taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag
      661 ttgatgttcg tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg
      721 accttcataa aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag
      781 aattagaaat gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg
      841 aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg
      901 caaatctcta attatatttt aaagaaatct gtctttatgg cagatttctt taatctcata
      961 taattcttat aaaaaatata atatttaact cgtcatattg ctta
//
LOCUS       DAEBXD010000339          983 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEBXD010000339 DAEBXD010000000
VERSION     DAEBXD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.347"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KJL94_004464"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KJL94_004464"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBD4412499.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAEBXD010000340          968 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEBXD010000340 DAEBXD010000000
VERSION     DAEBXD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.348"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..968
                     /locus_tag="KJL94_004465"
                     /pseudo
     CDS             1..968
                     /locus_tag="KJL94_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          225 a          243 c          316 g          184 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaag
//
LOCUS       DAEBXD010000341          936 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEBXD010000341 DAEBXD010000000
VERSION     DAEBXD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.349"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            34..783
                     /locus_tag="KJL94_004466"
     CDS             34..783
                     /locus_tag="KJL94_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBD4412500.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          264 a          198 c          203 g          271 t
ORIGIN      
        1 tataaatatt tatcctctat aaggagatgt tatatgaaaa gacagctttt tactcttagt
       61 attgtcgggg tattttcttt aaataccttt gcatcattcc ctccgggaaa tgatgtgaca
      121 acaaaacctg acctttacta cctgacaaac gataatgcta ttgacagtct ggcattatta
      181 ccgcctccac cacaaatcgg aagtattgcc ttcctgaatg accaggccat gtatgaaaag
      241 gggcgtttat tgcgaaatac tgaaagaggg aagctggcag ctgaggatgc caatctcagt
      301 agtggtggcg tggcaaatgt attttctgct gcttttggtt ctccgataac ggctaaagat
      361 tcaccagaat tgcataaatt actgacaaat atgattgagg atgccggaga cctggcaacg
      421 cgttcagcaa aagaatatta tatgcgtatt cgaccttttg ctttttatgg tgtttctacg
      481 tgtaatacaa aagaacagga tacattatcc agaaatggct cttatccatc aggtcataca
      541 tcgattggtt gggcaacagc acttgtattg tccgaaataa atccggcacg ccaggatacc
      601 attctgaaac ggggttatga actgggggac agcagggtta tttgcggtta tcactggcaa
      661 agtgatgttg acgcagcacg tattgttggc tctgctattg tagcaacact gcactcaaat
      721 cctgtgttcc aggcacaatt acaaaaagcg aaagatgaat tcgctaacaa tcagaaaaaa
      781 taatgctttt agccagtacg ccttataacc ccgaccttca gggctaatgc cagtcagtta
      841 agcaactgac tggctctttt tcagggctgt ggggtatttc cagggcctct cctttaccac
      901 tctcggaaag gccctttccc ttcttgtcgg taattt
//
LOCUS       DAEBXD010000342          933 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEBXD010000342 DAEBXD010000000
VERSION     DAEBXD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.350"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(55..243)
                     /locus_tag="KJL94_004467"
     CDS             complement(55..243)
                     /locus_tag="KJL94_004467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412501.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAEBXD010000343          916 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEBXD010000343 DAEBXD010000000
VERSION     DAEBXD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.351"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            32..457
                     /locus_tag="KJL94_004468"
     CDS             32..457
                     /locus_tag="KJL94_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4412502.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            504..>916
                     /locus_tag="KJL94_004469"
     CDS             504..>916
                     /locus_tag="KJL94_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBD4412503.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGET"
BASE COUNT          209 a          214 c          295 g          198 t
ORIGIN      
        1 gcggaatatg tgaacaacgg aggaaaacat catgcaatat gcgaaacctg tcactctgaa
       61 tgttgaagag tgcgaccgtc tctcctttct gccgtatctg tttggcaacg attttctgta
      121 tgccgaggcg tatgtgtacg cgctggcgca aaaaatgatg ccggaatatc agggaggctt
      181 ctggcacttc atccgcctgc cggacggtgg cggttacatg atgccggatg gcgaccgttt
      241 ccacatggtg aacggtgcaa actggtttga tcgtaccgtg agtgctgatg ccgcaggcat
      301 catcctgacc tcccttgtga ttaaccgcca gttgtggctg taccacgaca gcggggatgc
      361 aggactgacc cagctttacc ggatgcgcga tgcgcagttg tggcgtcaca tcgaatttca
      421 ccccgaatgc aacgcgattt acgcggctct ggactgatta acggacgggg cggcaatgcc
      481 gcccctgtaa aggagacgag aacatgtact gtactgttaa agaaattatc cgcgaagtgc
      541 tggatacaga cgtgccggac agtgaatgcg tttttgccgt agtgctgacc cgtggggatg
      601 tgcgtcacat agcccaggac tggagtctga cagacgatga gctggaaacc gtcatgcagc
      661 ggctggacga tgcctttgaa tatggtgcgg atgtcagcgt tgttcacggc gttgttcgtg
      721 aactgatgga agaaaagcgc gccagccgtc aggtgacagt cccggcggtg atgctggaaa
      781 aagtgatggc gctggcaggc agtgaaatga agcgcctgta tgccgtcggg agtgagaacg
      841 ggggcgacgg tgacgcattc gtcagggagg aacgcgaagc aatggacgtt gtgttacagg
      901 cgctggacgg ggagac
//
LOCUS       DAEBXD010000344          901 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEBXD010000344 DAEBXD010000000
VERSION     DAEBXD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.352"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..124
                     /locus_tag="KJL94_004470"
     CDS             <1..124
                     /locus_tag="KJL94_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4412504.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            239..>901
                     /locus_tag="KJL94_004471"
     CDS             239..>901
                     /locus_tag="KJL94_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBD4412505.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHS"
BASE COUNT          237 a          234 c          242 g          188 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctaaat agctgcgcgg aatagtagat ccctttgagg gaacttagcc
      181 cggattgtgc gatctgatca atcgccaaat caaaacaaat caccaaccgg actgagcaat
      241 gccgatcata gcaccaattt cccgtgacga acgacgcctg atgcagaaag ccatccataa
      301 aacacacgat aaaaattatg cccgcagact gactgccatg ctgatgctgc accggggcga
      361 ccgtgtcagc gacgttgcca gaacgctctg ctgcgcccgt tcctctgttg gacgctggat
      421 taactggttc acgcagtcgg gtgttgaggg actgaaatca ttacctgccg ggcgtgcccg
      481 tcgctggccg tttgagcata tctgcacact gttacgtgag ctggtaaaac attctcccgg
      541 cgactttggc taccagcgtt cacgctggag tacagaactg ctggcaataa aaatcaatga
      601 gataaccggt tgccagttaa atgccggaac cgttcgccgc tggttgccgt ctgcggggat
      661 tgtgtggcga agggctgcgc caactctgcg tatccgtgac ccgcataaag atgaaaagat
      721 ggcagcaatc cataaagcac tggacgaatg cagcgcagag catccggtct tttatgaaga
      781 tgaagtggat atccatcttc atcccaaaat cggtgcggac tggcaactgc gcggacagca
      841 aaaacgggtg gtcacgccgg gacagaatga aaaatattat ctggccggag cgctgcacag
      901 c
//
LOCUS       DAEBXD010000345          890 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEBXD010000345 DAEBXD010000000
VERSION     DAEBXD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.353"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(255..>890)
                     /locus_tag="KJL94_004472"
     CDS             complement(255..>890)
                     /locus_tag="KJL94_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4412506.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          221 a          199 c          188 g          282 t
ORIGIN      
        1 atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa aggggcaaaa aagagtgggt
       61 tatcgtagct tattgttgtc gctgataatg tttaacatat tgaaaaataa ataaaatact
      121 tatgagtcag atagttgtga tttttgccct tacttgttca ggtcgtattg ttctttcttt
      181 gtaatttgtt gattttcttg cattatttca gttctctggt actaaatggg gcaaattggg
      241 ggcaaacttt gcaactacga taaccgcgca ttcaacatgg ctatctgttc gtcgttcatg
      301 tcatcaatcc acataccgta aatttcatac accatttgcg cagtttcatg ccccatctgg
      361 ctggctataa atgccgggtt cgctcctgcc gtcaacagcc agcaggcaaa agtatgtcgc
      421 gtgtggtacg gattacggcg gcgaatacca gcacgtttta ctgctgcatt ccatcttgta
      481 cccaaactgc ttaccgagta ataaggtttc tgtttttcgt tacacaccct gggcatgaaa
      541 acaaaatgca gtttttgttt ttcagttctg ccgtactccc gatgataaaa agtgatttcg
      601 cttttgcgat gatgcccggt cagtttgtat tgctccttca gtgcttcaag agccggctgc
      661 agtaatgtta ccgtccggat cccggcattt gtttttgggg gaccgaacat atcaagtatc
      721 gtcaggtttc ttctgacatt cacaattccc ttctcgaaat ccacatcctc ccacgccaga
      781 gctgccagtt ccccgtgacg aagcccggag taaacggcaa atttccacaa gttttggctc
      841 tgtccttttt cactttccat taatgcattg aattctgttt tagataacgg
//
LOCUS       DAEBXD010000346          867 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEBXD010000346 DAEBXD010000000
VERSION     DAEBXD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.354"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(7..698)
                     /locus_tag="KJL94_004473"
     CDS             complement(join(7..452,452..698))
                     /locus_tag="KJL94_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4412507.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGCYTARTLKIGINTVIRTFKKLSPRRITSAPVAHADVA
                     LICELDEQWRFVGSKAQQHWPWYAYNTKTGGVLAYTCGPRNDETCRELLALLTPFCIG
                     MVTSDDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHE
                     KVIGSFIEKHIF"
BASE COUNT          218 a          205 c          225 g          219 t
ORIGIN      
        1 caatcattag aatatgtgct tttcaatgaa ggaaccgatg actttttcgt ggatttccac
       61 ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc ggagggtccg
      121 gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct ccttcggcac
      181 ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga acggagtcaa
      241 cagggctaac agttcccgac aggtctcatc attccgtggc ccgcaagtgt aagccaacac
      301 accgccagtt ttagtgttgt acgcatacca gggccagtgt tgttgcgcct tgctgccaac
      361 gaagcgccac tgctcatcaa gttcacagat aagggcaaca tcagcgtgag cgactggcgc
      421 agatgttatc cgccggggcg agagcttttt aatgtccgta tgacggtatt gatgccgatt
      481 ttcagtgttc tggcggtata acagccccct gcgccgttat gtgccatttc aacgatctgc
      541 tccttaacgc cgagttttct ggcttcataa gagtaagtga gctggaaaac acgtttacat
      601 gagcgacaac ggaaacgctc atgccgggaa caactacggc catgtcgata aacttcatcg
      661 gaattacagc gcggacaatg aacagtaacg gtagtcatgg aaaacctcaa ataccagtag
      721 attaccagaa acttctatcg attgaagaca ttaccttttg ccgcaatagg tggggtaaca
      781 ttggtgaaaa cgcattacgc cgggtggcag ttggaaaaaa attatctttt ttcggctctg
      841 atcgtggtgg agaaaatgcg gcaataa
//
LOCUS       DAEBXD010000347          852 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEBXD010000347 DAEBXD010000000
VERSION     DAEBXD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.355"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            60..557
                     /locus_tag="KJL94_004474"
                     /pseudo
     CDS             60..557
                     /locus_tag="KJL94_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            598..789
                     /locus_tag="KJL94_004475"
                     /pseudo
     CDS             598..789
                     /locus_tag="KJL94_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          221 a          211 c          249 g          171 t
ORIGIN      
        1 agcgccagcc ccgtcccgcc cgatagagcg cggcatagcg ggccccggat tacttgcgtg
       61 cgacggcaga agaagccctg aagcgaatca gtgaactgta cgccatcgag gatgaaatac
      121 ggggattacc agagtcagag tgtcttgcag tcaggcagca gcgaagcaaa gcgttactga
      181 cgtcgctgca tgaatggatg gtggagaaga atggcacgct gtcgaaaaaa tccagactgg
      241 gcgaagcatt cagctatgta ctgaatcagt gggacgccct ctgttattac agtgatgacg
      301 gtctggcgga ggcggacaat aacacagcgg aaagagcgct tcgtgcagtc tgtctcggaa
      361 agaaaaatta cgtgttcttc ggtagcgatc acggcggcga gcgtggtgca ctgctgtacg
      421 ggctgatcgg cacctgccgt ctgaacggta tcgatccgga agcgtatctg cgccatattc
      481 tgagcgtact gccggaatgg ccttccaacc gagttgacga cctcctgcca tggaaggtgg
      541 tgctaccatc aggataatca tcaatacggt gttcggtgaa cgtttacttg gagcatcatg
      601 cttcatgaag aaaaactggc acgtcatcaa cgcaaacagg cgatgtatac ccgaatggca
      661 gccttcccgg cggtgaaaat gttcgaagag tatgacttca cgttcgccac cggagcaccg
      721 cagaagcagc tccagtcgtt acgctcactc agcttcatag agcgcaatga aaacatagtc
      781 ttactgggga catctgacat cacaaatccc cgggtgggga tttgtgtata agagacagcc
      841 catggaggaa gg
//
LOCUS       DAEBXD010000348          839 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEBXD010000348 DAEBXD010000000
VERSION     DAEBXD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.356"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            42..806
                     /locus_tag="KJL94_004476"
     CDS             42..806
                     /locus_tag="KJL94_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBD4412508.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          178 a          237 c          276 g          148 t
ORIGIN      
        1 gaagcttgag catgcttggc ggagattgga cggacggaac gatgacggat ttcaagtggc
       61 gccatttcca gggtgatgtg atcctgtggg cggtgcgctg gtattgtcgc tatccgatca
      121 gctatcgcga ccttgaggaa atgctggcgg aacgcggcat ttcggtcgac catacgacga
      181 tctatcgctg ggtccagtgc tacgccccgg agatggagaa gcggctgcgc tggttctggc
      241 ggcgtggctt tgatccgagc tggcgcctgg atgaaaccta cgtcaaggtg cggggcaagt
      301 ggacctacct gtaccgggca gtcgacaagc ggggcgacac gatcgatttc tacctgtcgc
      361 cgacccgcag cgccaaggca gcgaagcggt tcctgggcaa ggccctgcga ggcctgaagc
      421 actgggaaaa gcctgccacg ctcaataccg acaaagcgcc gagctatggt gcagcgatca
      481 ccgaattgaa gcgcgaagga aagctggacc gggagacggc ccaccggcag gtgaagtatc
      541 tcaataacgt gatcgaggcc gatcacggaa agctcaagat actgatcaag ccggtgcgcg
      601 gtttcaaatc gatccccacg gcctatgcca cgatcaaggg attcgaagtc atgcgagccc
      661 tgcgcaaagg acaggctcgc ccctggtgcc tgcagcccgg catcaggggc gaggtgcgcc
      721 ttgtggagag agcttttggc attgggccct cggcgctgac ggaggccatg ggcatgctca
      781 accaccattt cgcagcagcc gcctgatcgg cgcagagcga cagcctacct ctgactgcc
//
LOCUS       DAEBXD010000349          832 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEBXD010000349 DAEBXD010000000
VERSION     DAEBXD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.357"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>832
                     /locus_tag="KJL94_004477"
     CDS             <1..>832
                     /locus_tag="KJL94_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD4412509.1"
                     /translation="PVLPACIKKLDVSCNKLTILTDLPENIKELIARDNFLTHISALP
                     HYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPS
                     LPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQI
                     TTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLR
                     QMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA
                     "
BASE COUNT          262 a          195 c          125 g          250 t
ORIGIN      
        1 tacctgtact ccctgcttgc attaaaaagc ttgatgtgag ctgtaataaa ttaaccatcc
       61 ttactgattt acctgaaaat attaaagaac ttattgcaag agataatttc ttaacacata
      121 tatctgcatt accacattat ctaataactt tggatgtgtc cgaaaatcaa ttagagaatc
      181 tgccgttatt accagacacc atcaaatcac taagcgcaga gtataatagg ttatccacac
      241 tgccttcatt acccttgaat ttaaaaaaac ttgaggttag gaacaacgaa ctgcaaactc
      301 ttccatctct gccttctaat cttaagatac ttaaggttgc gcacaaccat cttactgaac
      361 tgcccccttt acctaggaga ctgcaacttc tttttgcata tagcaataga ttaagcaact
      421 taccaaacat ccaagaaaat attatcatga gaagattttt ttattttgaa aacaaccaaa
      481 taactacaat cccgacaaat ctttttcgtt tagatcctca tataactatt gagattgcaa
      541 ataacccctt atcagatcaa actctgctat tcttaataca gcaaacttcg gttccaaatt
      601 ttaacgggcc tcagtttcgt atttccctgt cagaccaaaa cagactgttt ttacgccaga
      661 tgttgccgca aaatttacat tcgcgccata tcagagtcat cactgaaggg gggcagaact
      721 ttcagatccc ccctcttccc gaaactgtgg cagcctggtt tcctgaagca gatcgtcggg
      781 aggtttctac acaatggact tctttttcca ccgaggagaa ttcccgggca tt
//
LOCUS       DAEBXD010000350          831 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEBXD010000350 DAEBXD010000000
VERSION     DAEBXD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.358"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..326
                     /locus_tag="KJL94_004478"
     CDS             <1..326
                     /locus_tag="KJL94_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD4412510.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KJL94_004479"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KJL94_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBD4412511.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          154 g          241 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAEBXD010000351          826 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEBXD010000351 DAEBXD010000000
VERSION     DAEBXD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.359"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(570..749)
                     /locus_tag="KJL94_004480"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="KJL94_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          227 a          136 c          206 g          257 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagact
//
LOCUS       DAEBXD010000352          812 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEBXD010000352 DAEBXD010000000
VERSION     DAEBXD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.360"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..366)
                     /locus_tag="KJL94_004481"
     CDS             complement(<1..366)
                     /locus_tag="KJL94_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4412512.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEG"
     gene            complement(378..779)
                     /locus_tag="KJL94_004482"
     CDS             complement(378..779)
                     /locus_tag="KJL94_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD4412513.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          200 a          252 c          168 g          192 t
ORIGIN      
        1 cccttcataa tatcgtcctg cccttacgtt tcccggcagc ccctcacgcc gcaaccggat
       61 ataactacat ccgccatcat caggggtgat acgatccaca cagaaagact cacgaccaat
      121 catcagcgaa tcattgcgac acagccgtaa aatatctgct gttttcacaa acagggatgg
      181 taaagagtct tcaatacgca caccgccggc caccagagaa atattttcag gatcatcaaa
      241 aatcccggaa atcatgatcc cttttagccg gcctgacgta atgaccgctc taatccccat
      301 attgcgaata atggccttgt ctgcaagatc aagtgcaaca tcaaacagat tatcgaaatc
      361 agccacgctg cctcccgtta ttgcattctg gccaggccac gttctgtcat tccggctgcc
      421 acaccggcaa agacacggaa cgccattccc ggcggcacaa atgccagagg ttcatcccgc
      481 gtggcatgaa gtgcatcggt atgcagcgtc accagtgcca cgactgtaac cagatcatcc
      541 atattctgaa tcacggtctc cggctgcgct gatatcacct cattttcatg tccggtcagc
      601 gcattttccg ggtgggcaga cttatcccgg atgtcagtgt catccatatt ggcaagttcc
      661 tcctccagct cagccacacg ctgcgcaata tctgctgcgc ttcccgagat atccggctca
      721 cgtcccagcg atgctgccag ttctctcaga cgtttcaggt tatcgtcttt ctttgccata
      781 tctgttcctg tccgaataaa aaacgggagc cc
//
LOCUS       DAEBXD010000353          789 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEBXD010000353 DAEBXD010000000
VERSION     DAEBXD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.361"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..789
                     /locus_tag="KJL94_004483"
                     /pseudo
     CDS             1..789
                     /locus_tag="KJL94_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          242 a          144 c          174 g          229 t
ORIGIN      
        1 aataaacaaa tcaccgctaa aggtggctgg aaagctattt cagaaagtac acaggcacaa
       61 ctagggaact tgaaaaactc ttggaatacc acgcttgata gtatcttcag aggttctgaa
      121 aatggtctgc aagattttac aagaagctta accaatcttt taaacgctct tggtggaagt
      181 ggtaagagtc ttggtgaatc tcttggtagt ctcatgactt ctatgtcaca tggtgtcgat
      241 tcattgaccg atattagtta cagggtaagg gcgttctttg atgaggtgac attagcttac
      301 cgtccactaa atgacactca gaggaaaatt gttgatggtt tatctggtgg gttaattgat
      361 ggtctgaaga tgcttgctgt ggctgttact gctcaaaagg ctgcaaatgt agcaggaggc
      421 ttgttaaacc ttgcaaacgc tgttacttca ttcggtgacg ctgttaataa agataaagct
      481 ggcaaggctg gttcttcagg tggtgcgcta aataaagtcg gtagagttgt cctttatgct
      541 gagttggtaa attttgctct tgataattta aaccctgaag cagctaagaa tccgaactta
      601 catcaatcag gtattggcac agaaacaaac ttcaaccctg aatacaaagg tgattcatct
      661 aagccaatag ttctatcgct gactggttta atggtgcaac taataaaatt gctaacttcc
      721 aagaaccgat gaataacttc gtaaataaca cacaatcaac tccatcagct atcacaggtg
      781 acatggtta
//
LOCUS       DAEBXD010000354          788 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEBXD010000354 DAEBXD010000000
VERSION     DAEBXD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.362"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="KJL94_004484"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="KJL94_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBD4412514.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          193 a          207 c          176 g          212 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaa
//
LOCUS       DAEBXD010000355          755 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEBXD010000355 DAEBXD010000000
VERSION     DAEBXD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.363"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(137..754)
                     /locus_tag="KJL94_004485"
     CDS             complement(137..754)
                     /locus_tag="KJL94_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD4412515.1"
                     /translation="MSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDR
                     DQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRT
                     EQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSMPE
                     LEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          146 a          239 c          179 g          191 t
ORIGIN      
        1 gaggcccgcc cccgggcctc cccggattca tccgtttctc tgttcagcct gaccgcacac
       61 cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag cggtcttctg tgattccgcc
      121 agttgcggct tattcattac ttaacgtcaa acgcccgaat tgaagccaaa tcatccagac
      181 tgctcagctc ctctttcatc tcccgctgac ggcgataaat ctcatcgttg cgatcgacct
      241 gcgcctgcac cattgctgcc gccagttctt ccagttccgg catcgacagt ttcacctgct
      301 gattatccgc atcactccac accatatgtg tttgtgccgc gacagatttt gccagcatga
      361 ccaccgggga caggcgggca actgacgagg cgtcagcgtt ccacgtgcgg ccgttccatt
      421 caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc ttcaatttcc tgacgtctgg
      481 cctctctggc cgcttccagc atttctggtg tcacagtgaa tggggctatc tcaccccatt
      541 tgccactttg cagttcctgc cagatttgct gacccgtcgg tgcaacatca tcagcggtgg
      601 ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac gtcacagtct actgcgccac
      661 tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg
      721 cgaataaaaa gcccgggcat tgcgccagag acatg
//
LOCUS       DAEBXD010000356          747 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEBXD010000356 DAEBXD010000000
VERSION     DAEBXD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.364"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>747
                     /locus_tag="KJL94_004486"
     CDS             <1..>747
                     /locus_tag="KJL94_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBD4412516.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAEBXD010000357          693 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEBXD010000357 DAEBXD010000000
VERSION     DAEBXD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.365"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..525
                     /locus_tag="KJL94_004487"
                     /pseudo
     CDS             1..525
                     /locus_tag="KJL94_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="KJL94_004488"
     CDS             589..>693
                     /locus_tag="KJL94_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4412517.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAEBXD010000358          692 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEBXD010000358 DAEBXD010000000
VERSION     DAEBXD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.366"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            36..>692
                     /gene="istA"
                     /locus_tag="KJL94_004489"
     CDS             36..>692
                     /gene="istA"
                     /locus_tag="KJL94_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBD4412518.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccggac gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DAEBXD010000359          687 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEBXD010000359 DAEBXD010000000
VERSION     DAEBXD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.367"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..667)
                     /locus_tag="KJL94_004490"
     CDS             complement(<1..667)
                     /locus_tag="KJL94_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBD4412519.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          173 c          154 g          175 t
ORIGIN      
        1 ttgccgcatc atcatcagac tccggaacgc cctcatacac atgtttaaaa ccatccggat
       61 cacggcgtcg cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt
      121 catagttaat tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga
      181 cgaaatcagt aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga
      241 tcgttggctc cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccagccaca
      301 acatatcgat cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa
      361 caaattccga gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc
      421 gcagaccaaa cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat
      481 tctggatctg acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa
      541 tgccagcggc atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg
      601 cccgcatagt ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag
      661 aagtcattga ggtcacgctc tccattg
//
LOCUS       DAEBXD010000360          683 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEBXD010000360 DAEBXD010000000
VERSION     DAEBXD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.368"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>683
                     /locus_tag="KJL94_004491"
     CDS             <1..>683
                     /locus_tag="KJL94_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD4412520.1"
                     /translation="TLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFLE
                     FLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLS
                     KVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLAC
                     YQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSVDSTI
                     TDLLEKALN"
BASE COUNT          238 a           99 c          147 g          199 t
ORIGIN      
        1 ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg tatgtgggaa
       61 tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt ctttctccta
      121 gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat tttagcaaac
      181 gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct tattctaaag
      241 atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg gagcgagcgg
      301 atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa gggattcgtg
      361 aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt tatgaaggga
      421 agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag gttctggcgt
      481 gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat cagaagttaa
      541 ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag ttaaataatt
      601 cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct acgattacgg
      661 atttattaga aaaagcattg aat
//
LOCUS       DAEBXD010000361          665 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEBXD010000361 DAEBXD010000000
VERSION     DAEBXD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.369"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            1..665
                     /locus_tag="KJL94_004492"
                     /pseudo
     CDS             1..665
                     /locus_tag="KJL94_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          170 a          170 c          180 g          145 t
ORIGIN      
        1 ataaagaagt acggtccgga ttcttccact aataaagaac agtcagatgc tcaggccgag
       61 atccgccgtc tccagaaaga gctgaaacgg gttaccgacg aacgggacat attaaaaaaa
      121 gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc ctttatccgt gacaactcct
      181 gttgctggcc tgttcgcctg ctctgtcggg tgctggatgt tcatcccagt ggtttttacg
      241 cctggcttca gcagccgcat tcacaacgcc atcaggcaga cctgagactg acaggacaga
      301 ttaaacagtt ctggctggaa tcgggatgcg tctatggtta tcgcaaaatc catctggatc
      361 tgcgtgacag cgggcaacag tgcggagtaa acagagtctg gagactgatg aaacgtgtcg
      421 gaataaaggc tcaggtcgga taccgaagcc cgcgggcacg taaaggcgag gccagtatcg
      481 tgtcacccaa caggctccag cgacagttca atccggatgc tcctgatgag cgttgggtaa
      541 cggacataac ctacatcagg acccacgaag gctggctgta tcttgccgtt gttgttgatc
      601 tgttctcacg caaaattatc ggctggtcca tgcaatcccg gatgacaaag gacattgtcc
      661 tgaac
//
LOCUS       DAEBXD010000362          638 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEBXD010000362 DAEBXD010000000
VERSION     DAEBXD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.371"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..>638)
                     /gene="ipaH7.8"
                     /locus_tag="KJL94_004493"
     CDS             complement(<1..>638)
                     /gene="ipaH7.8"
                     /locus_tag="KJL94_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBD4412521.1"
                     /translation="YLRILTEWEKNSSPGEERGIAFNRLSQCFQNQEAVLNLSDLNLT
                     SLPELPKHISALIVENNKLTSLPKLPAFLKELNADNNRLSVIPELPESLTTLSVRSNQ
                     LENLPVLPNHLTSLFVENNRLYNLPALPEKLKFLHVYYNRLTTLPDLPDKLEILCAQR
                     NNLVTFPQFSDRNNIRQKEYYFHFNQITTLPESFSQLDSSYRINISGNPLST"
BASE COUNT          206 a           97 c          128 g          207 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatattaa tcctgtaact tgaatctaat tgtgaaaaac
       61 tctccggaag agtggttatc tgattaaaat gaaaataata ttccttttgt ctgatattgt
      121 ttctatcaga aaattgagga aaagtaacca gattattgcg ctgagcacag agaatttcca
      181 gtttatccgg taagtcgggt aatgttgtca gcctgttata ataaacatgt aaaaatttca
      241 atttttcggg aagagccggt aagttatata gcctgttatt ttcaacaaat aatgatgtta
      301 aatggtttgg caaaacagga aggttttcca gttgattaga acgaacactt aaagttgtta
      361 atgactcagg aagttctggt atcacagaaa gcctgttatt atcagcatta agttctttaa
      421 gaaatgcagg cagctttggc aatgatgtta atttattatt ttctacaatc aaagcagaaa
      481 tatgctttgg taattcggga agagacgtca aatttaggtc tgataaattt aatactgctt
      541 cttgattctg aaagcactgg gagagtctgt taaaagcaat gcctcgctct tccccgggag
      601 aagagttctt ttcccattca gtcaggattc tcagataa
//
LOCUS       DAEBXD010000363          636 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEBXD010000363 DAEBXD010000000
VERSION     DAEBXD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.372"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            19..492
                     /locus_tag="KJL94_004494"
     CDS             19..492
                     /locus_tag="KJL94_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4412522.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          128 g          185 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcagaaaa tgctcg
//
LOCUS       DAEBXD010000364          629 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEBXD010000364 DAEBXD010000000
VERSION     DAEBXD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.374"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KJL94_004495"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KJL94_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBD4412523.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..555)
                     /gene="csgA"
                     /locus_tag="KJL94_004496"
     CDS             complement(190..555)
                     /gene="csgA"
                     /locus_tag="KJL94_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBD4412524.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          164 a          142 c          150 g          173 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattact
      541 gaggtgtact ggcaatagcg gacactacca tttgttcttt tttaagcagc catctgatga
      601 tatttttccc tgaaggctgc cggggagat
//
LOCUS       DAEBXD010000365          606 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEBXD010000365 DAEBXD010000000
VERSION     DAEBXD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.375"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            62..606
                     /locus_tag="KJL94_004497"
                     /pseudo
     CDS             62..606
                     /locus_tag="KJL94_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          140 a          156 c          159 g          151 t
ORIGIN      
        1 acgaatcgga aaatcgatac tggaaaaact ctaactcggt ggccagtttt tgttgaccac
       61 ttcaggtcag gagattgtcg tcgaaccgat tgaacaggct attctcatct ctgagcagac
      121 taagctgtgg gacgccaagt catccaccgg attcactcag ggcacacttc agcaggacgc
      181 tatgaacatc tgtgagaatg tggtccgtac catcaacgct cgcatggtcg acgttctgga
      241 atcctctaag ctgctgaaga ccgtagagct tcctgtcctg actggtagcc tgaccgctaa
      301 ggcagacgct atcatggacg ctctgtatga gaacaccgag ccttctttcg gttctgaggt
      361 gtctgactac ggtattatcg cccatgagag ccaactgaaa gcactgtccc gtctggctgc
      421 taaacagggc ttctctggtg aagacgctat cgtggatatg ctgggtaccg acatcgcgta
      481 ttacaacggt gaggacaaag gtgtgttcat gttggctaaa cgcttcacgg ccctgagctt
      541 cggctgcttc cgtcacgacg gtgagaatat tacggtcgtc ctttcacgtg atggcgactc
      601 tcagtc
//
LOCUS       DAEBXD010000366          591 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEBXD010000366 DAEBXD010000000
VERSION     DAEBXD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.376"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(102..>591)
                     /locus_tag="KJL94_004498"
     CDS             complement(102..>591)
                     /locus_tag="KJL94_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4412525.1"
                     /translation="QVKDVRTTEAGTVYIHINEDDSSLPGKSIKNAHSDRCVPLVDGA
                     YGFILADFMALVETRRGADGDDAMVFDGLRLMKNGYGEQVSKWFNRTLLPKVLVDRSG
                     LAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITYDRYAKEVEVDRLVNVMADVYK
                     ET"
BASE COUNT          162 a          168 c          114 g          147 t
ORIGIN      
        1 gtgagtcgta ttaattatcc cttgacaagt agagaaaacc atgagtcaaa aacatgacca
       61 cacaggtctg tagtaagtgt catcctagac tatggtctcc tctaggtctc cttataaaca
      121 tcagccatca cattgaccag cctatcaacc tcgacctctt tagcataacg gtcgtacgta
      181 atagaccctg aactatgccc catgatcgcc tgtgcatacg ccaactcgac cccatgttgt
      241 tttaactggg tcgcaactgt atgtctaaac gagtgaaacg ctaagccgct acggtctacg
      301 aggactttag gcaacaacgt ccgattaaac catttactca cctgctcacc gtaaccgttc
      361 ttcatgagac gcagaccatc gaacaccata gcgtcatcac cgtcagcgcc tctacgggtc
      421 tctacaagcg ccataaagtc agccaatata aaaccatagg ccccatcgac cagaggcaca
      481 cagcggtcac tatgagcgtt cttgatgctc ttaccgggta gactactatc gtcttcattg
      541 atatgaatat aaacggttcc cgcctcagtg gtcctgacgt ctttgacctg t
//
LOCUS       DAEBXD010000367          587 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEBXD010000367 DAEBXD010000000
VERSION     DAEBXD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.377"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>587
                     /locus_tag="KJL94_004499"
     CDS             <1..>587
                     /locus_tag="KJL94_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD4412526.1"
                     /translation="RLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAG
                     TDLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTI
                     DGFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDG
                     DVVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          153 a          115 c          145 g          174 t
ORIGIN      
        1 cgtttggcat atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat
       61 gtcggtgtcg cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac
      121 tcatgggctg gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt
      181 aacacagatt tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa
      241 aatgaaggta caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac
      301 actattgacg gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag
      361 tctggtgatg gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct
      421 aacaatgttt atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt
      481 gatgttgtga acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt
      541 ctgcgtccat ccatcggtta ctcctattct aaagcactgg acgtagc
//
LOCUS       DAEBXD010000368          569 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEBXD010000368 DAEBXD010000000
VERSION     DAEBXD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.380"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..424)
                     /locus_tag="KJL94_004500"
     CDS             complement(<1..424)
                     /locus_tag="KJL94_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412527.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIATI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNG"
BASE COUNT          170 a           81 c          103 g          215 t
ORIGIN      
        1 gaccattaag tttaattctt gggaattctt tttcttttct atctgatttt aggtttaatt
       61 cttgtttatc aagagtgtct gagcgttgag gtaaaatttc cattaggtat tcttcaaata
      121 ttgtagcaat ggtttgagtg agtgtcgtgt tttcgttttt attaattatc gtaatttctt
      181 tcttatcagc ttgtaagacg gacgtggaaa atttaatgac aaaatccttg ttgctaaaga
      241 tatgacgaac attttgtcta cagttctctg agaagctttt taatagaggc gtcgccagct
      301 ccttgccaga aaatttatcc tcgagttctt tataaaacaa ttcactcagg gtttggtgtt
      361 catttgtctg ggctgtatta ttaatatttg cagagaaaga actacgagga atacgagtaa
      421 tcatttaaat atctcattgt ttattgatgt cttgaatttt aactatcaga acagtgaaaa
      481 aatttaatat gattacaact aaaaaaatat cataaatcgc tcaatctcat aatgcagccg
      541 taaaagtttc ggtggaatga gatcttgcg
//
LOCUS       DAEBXD010000369          566 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEBXD010000369 DAEBXD010000000
VERSION     DAEBXD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.381"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..297
                     /locus_tag="KJL94_004501"
     CDS             <1..297
                     /locus_tag="KJL94_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBD4412528.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="KJL94_004502"
     CDS             complement(405..>566)
                     /locus_tag="KJL94_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412529.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAEBXD010000370          544 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEBXD010000370 DAEBXD010000000
VERSION     DAEBXD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.382"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
BASE COUNT          178 a           80 c          107 g          179 t
ORIGIN      
        1 atcattaact tatatgaata tttcagagtt ggagttcgaa tgttgacacc tgagtatttc
       61 ctgctaaaaa tagccctgct ttacggatgg ttgtattgca ataacacgaa agtagtaaga
      121 gattaaaggt atctgtcagc gataactctg caagtgatga cattttgtaa accaaataat
      181 gtaaatcagc aatgtatctt ttcacttaat tataacaaat gtggtattca ctaagaatct
      241 gatacgagct tacatcacga ctattttagt ctcatcgcgg gatacaggga tgcaccgatg
      301 gatctcagca caccgtaatg caatacctga acaaatagaa cagtaaagtg gttcagtatt
      361 aatgaaagct cttattttcc gttgggaaaa cttaaaatag atgagaaggc tttttgattt
      421 cttatgtgat ctcatttcga gtattgagaa atttgtgatt tttaaatttt tcaataagcc
      481 ctgtaaggga ttggggtttt atgagaaata aagagacgta ttgtctttgt ttaaacggta
      541 cgta
//
LOCUS       DAEBXD010000371          535 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEBXD010000371 DAEBXD010000000
VERSION     DAEBXD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.383"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..>535)
                     /gene="intI1"
                     /locus_tag="KJL94_004503"
     CDS             complement(<1..>535)
                     /gene="intI1"
                     /locus_tag="KJL94_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBD4412530.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWLKDQAEGRSGVALPDA"
BASE COUNT          116 a          183 c          153 g           83 t
ORIGIN      
        1 gggcgtcggg aagcgcaacg ccgctgcggc cctcggcctg gtccttcagc caccatgccc
       61 gtgcacgcga cagctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc
      121 gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct
      181 tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga
      241 acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca
      301 ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg
      361 tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga
      421 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca
      481 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggac
//
LOCUS       DAEBXD010000372          528 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEBXD010000372 DAEBXD010000000
VERSION     DAEBXD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.385"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            110..>528
                     /gene="ospC2"
                     /locus_tag="KJL94_004504"
     CDS             110..>528
                     /gene="ospC2"
                     /locus_tag="KJL94_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBD4412531.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDF"
BASE COUNT          197 a           90 c           79 g          162 t
ORIGIN      
        1 gtacaacata ttaaaatatg ttcgttataa tacatacaag aaaatattaa gttagataat
       61 attattctaa ataactgcag ataaaaacgc acataatggg ggatgtttta tgaaaatacc
      121 tgaagcagta aatcatatta atgttcagaa caatattgat cttgttgatg gaaaaataaa
      181 tccgaacaaa gatacaaaag cattacaaaa aaacatatca tgcgtaacaa actcatcctc
      241 ttctggcata agtgaaaaac atttagatca ctgtgctgat actgtgaaaa gctttttaag
      301 gaagtctata gcagcgcaaa gttatagtaa aatgttctct caagggacca gtttcaaatc
      361 attaaatctt tctattgaag cgccatctgg agcacggtca tcatttcggt cacttgagca
      421 cctcgataag gtgtccagac actatctttc tgaaataata caaaaaactc atcctctttc
      481 ttcggatgaa cgtcacttgc tctctattat tattaattct gattttaa
//
LOCUS       DAEBXD010000373          520 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEBXD010000373 DAEBXD010000000
VERSION     DAEBXD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.386"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            360..>520
                     /locus_tag="KJL94_004505"
     CDS             360..>520
                     /locus_tag="KJL94_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD4412532.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEEL"
BASE COUNT          139 a          138 c          106 g          137 t
ORIGIN      
        1 tttataataa tcaatcacca aacctatagg tcttctctag ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggccctgta
      121 cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga gaccctagag
      181 tggctcctag agagaccctc ccagtagctc cgcacagcta catcgtcatc atcatagtat
      241 tcaatctcaa agtcattctg tttgcatcct gttgtcctgc tggtctccct tcgcttgggg
      301 actttgagta ggtcagtggg ggtgcgactt cagggtgcaa acaaaaagga aatcaagcga
      361 tgtctcaaca accacaacaa ccacaacaac cacaacaaga aatcagcatc attaacctcg
      421 accagcttgt gtccatgacc tcagttgaaa ttgctgagct gacaggcaag gagcaccgaa
      481 atgtccttcg tgatattcgt aagatgctcg aagaacttaa
//
LOCUS       DAEBXD010000374          516 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEBXD010000374 DAEBXD010000000
VERSION     DAEBXD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.387"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>516
                     /locus_tag="KJL94_004506"
     CDS             <1..>516
                     /locus_tag="KJL94_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBD4412533.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLT"
BASE COUNT          113 a          163 c          139 g          101 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgtt
//
LOCUS       DAEBXD010000375          501 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEBXD010000375 DAEBXD010000000
VERSION     DAEBXD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.388"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>501
                     /locus_tag="KJL94_004507"
     CDS             <1..>501
                     /locus_tag="KJL94_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4412534.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRR"
BASE COUNT          177 a          110 c          120 g           94 t
ORIGIN      
        1 ggctcgaaga gaaagcacat aagaaatcac tggacgatga caaaagccgg atcggattct
       61 ggcttcaaca tttcgcaggg atgcaactaa gagacatcac tgaatcaaaa atttattcag
      121 caatgcagaa aatgacgaac cggcgtcatg aggaaaactg gaaactcagg gcagaagcat
      181 gcagaaaaaa agggaaacct gttccagaat acacgccaaa accagcgtcc gttgcaacga
      241 aggctacgca tctttcattt ataaaggccc tactaagagc cgcagagcgt gaatggaaaa
      301 tgctggataa ggcaccaatt attaaagtgc ctcaaccaaa gaataaacgg atccgctggc
      361 tggagcccca tgaagcacaa aggctgattg atgaatgtcc ggagccatta aagtctgttg
      421 ttgaatttgc actggcaaca ggcttaagac gctcgaacat catcaacctt gaatggcaac
      481 aaatagatat gcagcgccgg g
//
LOCUS       DAEBXD010000376          485 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEBXD010000376 DAEBXD010000000
VERSION     DAEBXD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.389"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..>485
                     /locus_tag="KJL94_004508"
     CDS             <1..>485
                     /locus_tag="KJL94_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBD4412535.1"
                     /translation="KELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCG
                     DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMF
                     HSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSF
                     S"
BASE COUNT          114 a          116 c          155 g          100 t
ORIGIN      
        1 aaagagctgg ggctggtcag ctgtcagcag ccgactcacc ggtataaacg tggtggtcat
       61 gaacatgttg ctatccctaa ctaccttgaa aggcagttcg ccgtgaccga gccaaatcag
      121 gtgtggtgcg gtgatgtgac ctatatctgg acgggtaagc gctgggcgta cctcgccgtt
      181 gttctcgacc tgttcgcaag aaaaccagtg ggctgggcca tgtcgttctc gccggacagc
      241 aggctcacca tgaaagcgct ggaaatggca tgggaaaccc gtggtaagcc cggcggggtg
      301 atgttccaca gcgatcaggg cagtcattat acgagcaggc agttccggca gttattgtgg
      361 cgataccaga tcagacagag tatgagccgg cgcggaaact gctgggataa cagcccaatg
      421 gaacgcttct tcaggagtct gaagaacgaa tggatgccgg tggtgggtta cgtaagcttc
      481 agcga
//
LOCUS       DAEBXD010000377          485 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEBXD010000377 DAEBXD010000000
VERSION     DAEBXD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.390"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..425
                     /locus_tag="KJL94_004509"
     CDS             <1..425
                     /locus_tag="KJL94_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4412536.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          139 a          106 c          109 g          131 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 atttt
//
LOCUS       DAEBXD010000378          484 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEBXD010000378 DAEBXD010000000
VERSION     DAEBXD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.391"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..>484)
                     /locus_tag="KJL94_004510"
     rRNA            complement(<1..>484)
                     /locus_tag="KJL94_004510"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a          144 c          118 g          121 t
ORIGIN      
        1 ggtgtgtaca aggcccggga acgtattcac cgtggcattc tgatccacga ttactagcga
       61 ttccgacttc atggagtcga gttgcagact ccaatccgga ctacgacgca ctttatgagg
      121 tccgcttgct ctcgcgaggt cgcttctctt tgtatgcgcc attgtagcac gtgtgtagcc
      181 ctggtcgtaa gggccatgat gacttgacgt catccccacc ttcctccagt ttatcactgg
      241 cagtctcctt tgagttcccg gccggaccgc tggcaacaaa ggataagggt tgcgctcgtt
      301 gcgggactta acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc
      361 acggttcccg aaggcacatt ctcatctctg aaaacttccg tggatgtcaa gaccaggtaa
      421 ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca
      481 attc
//
LOCUS       DAEBXD010000379          481 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEBXD010000379 DAEBXD010000000
VERSION     DAEBXD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.393"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            52..481
                     /locus_tag="KJL94_004511"
                     /pseudo
     CDS             52..481
                     /locus_tag="KJL94_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          156 a          118 c          112 g           95 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 g
//
LOCUS       DAEBXD010000380          478 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEBXD010000380 DAEBXD010000000
VERSION     DAEBXD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.394"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            276..>478
                     /locus_tag="KJL94_004512"
     CDS             276..>478
                     /locus_tag="KJL94_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD4412537.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALA"
BASE COUNT          167 a           94 c           86 g          131 t
ORIGIN      
        1 atattaccaa gtcaagtagt caggctggct aaaatagttg gagatgttgt agtatcttac
       61 tcaagactaa tgtcttctgg tataaatcct ctgcattgtg aatatgtaaa acatcaaaaa
      121 aagatgaacc aaatatacga aaaaagcaaa tatacttacc agggtagaaa gcatgatata
      181 acactcattc ccttatggca aatattctga aacaccctcc tcattaggag agatacatta
      241 aatacaattt attcacaatc taattccata tccatatgcg agaaattaat atgctcagaa
      301 atatttcatc ctgtttattt ccacatatca gcacaattac atcccccaac cattatttgt
      361 ccgaatggga tgattgggag aaacaggggt taccggaaga acagcgtact gaggcggtaa
      421 gaagacttcg tgcatgtctt acctctaagg ggcataaact ggacctgcga gccttggc
//
LOCUS       DAEBXD010000381          460 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEBXD010000381 DAEBXD010000000
VERSION     DAEBXD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.395"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            119..>460
                     /locus_tag="KJL94_004513"
     CDS             119..>460
                     /locus_tag="KJL94_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412538.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT          147 a           70 c          101 g          142 t
ORIGIN      
        1 ttacgcgacg ttgaagttac ttgttttata atgagcgatt tttgatatct cgccgagaaa
       61 attatcagca ttctctcgtt tcagtgtcgt gaatatactg ttaacggagg tgaaaagaat
      121 gctgggggtt atacaaaacc tatgttgttg tcgtccggat tcaccgcttg ctgatagtaa
      181 gcagcgtaac cagaagttac cgcaacaaga ctttgaggat tttaagaacg acgttattaa
      241 tgtcattaac aacaatgaag atatttattt tcttagagag agtaatttac tgaatgcgtt
      301 tattcggacg tgcaaaaaaa gagatgataa cagttacgga aatatgatat ttacgcttta
      361 ttttgataac gatatctatg ggaacagaat tatcaaagca tcgctgaacg gcggagagtt
      421 gcagttatta agcacagggt gtagcaatga taacattctc
//
LOCUS       DAEBXD010000382          446 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEBXD010000382 DAEBXD010000000
VERSION     DAEBXD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.397"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..395)
                     /locus_tag="KJL94_004514"
     CDS             complement(<1..395)
                     /locus_tag="KJL94_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412539.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          105 a          123 c          123 g           95 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaac
//
LOCUS       DAEBXD010000383          444 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEBXD010000383 DAEBXD010000000
VERSION     DAEBXD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.398"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..373)
                     /locus_tag="KJL94_004515"
     CDS             complement(<1..373)
                     /locus_tag="KJL94_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4412540.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           95 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcg
//
LOCUS       DAEBXD010000384          444 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEBXD010000384 DAEBXD010000000
VERSION     DAEBXD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.399"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..>444)
                     /locus_tag="KJL94_004516"
     CDS             complement(<1..>444)
                     /locus_tag="KJL94_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBD4412541.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          101 a          113 c           86 g          144 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaa
//
LOCUS       DAEBXD010000385          439 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEBXD010000385 DAEBXD010000000
VERSION     DAEBXD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.401"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..202)
                     /locus_tag="KJL94_004517"
     CDS             complement(<1..202)
                     /locus_tag="KJL94_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBD4412542.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
     gene            302..>439
                     /locus_tag="KJL94_004518"
     CDS             302..>439
                     /locus_tag="KJL94_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4412543.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          116 a          104 c           92 g          127 t
ORIGIN      
        1 gagaataacg tttatccata ttcttccagt ctggttcatc ttccgacgtc atcaccgggt
       61 tgaaattacg ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct
      121 gatggatcgc atcagcaata tttgcggtca atgaatcatg acgaggatgg gcccatacca
      181 aataaatacg ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac
      241 gcaggcggca taatcctgta aacgtgatga ttcaggatga gttaatgaag gattttcact
      301 aatgcggatg aatatgtctg actttgccac ttcctttgcc gtggcccgta atcaaagctt
      361 tcgtgcagca ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac
      421 actggaacaa cgtcttaaa
//
LOCUS       DAEBXD010000386          419 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEBXD010000386 DAEBXD010000000
VERSION     DAEBXD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.denovo.402"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            60..400
                     /locus_tag="KJL94_004519"
                     /pseudo
     CDS             60..400
                     /locus_tag="KJL94_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          143 a           90 c           97 g           89 t
ORIGIN      
        1 ttgttctgag gtgagcatat cacctctgtt caggtggcca aattcagtgt gccacttcaa
       61 acagtgaatt atttgccaac gacggtacac cgacatggct aaaagaaaag cgtgcaaaag
      121 aaatcgcact tgatgaaatt aacttgatga aattatcgag ttgctcaaca agcaccatgg
      181 gggcaccagt aacgacgcca aacgcgcaaa agctgatttg ctggaaaaaa cattcacctc
      241 ccgctcatgg gaaagaatca aaggtatgga ctggccgaca atcaaagctg gtcgtaatgc
      301 actatggatt gaactggaag gcgttgaata tgcattcccg gatccacaaa cacaaaacga
      361 aacgcaacaa gcaggatacg atgagaatat tccggtgtaa aaccaagtgc attttggga
//
LOCUS       DAEBXD010000387         5949 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.176, whole genome shotgun sequence.
ACCESSION   DAEBXD010000387 DAEBXD010000000
VERSION     DAEBXD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.176"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            51..710
                     /locus_tag="KJL94_004520"
     CDS             51..710
                     /locus_tag="KJL94_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBD4412544.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(911..1288)
                     /locus_tag="KJL94_004521"
     CDS             complement(911..1288)
                     /locus_tag="KJL94_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4412545.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1355..4321)
                     /locus_tag="KJL94_004522"
     CDS             complement(1355..4321)
                     /locus_tag="KJL94_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBD4412546.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4324..4884)
                     /locus_tag="KJL94_004523"
     CDS             complement(4324..4884)
                     /locus_tag="KJL94_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4412547.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5010..5360)
                     /locus_tag="KJL94_004524"
     CDS             complement(5010..5360)
                     /locus_tag="KJL94_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBD4412548.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5563..>5949)
                     /gene="intI1"
                     /locus_tag="KJL94_004525"
     CDS             complement(5563..>5949)
                     /gene="intI1"
                     /locus_tag="KJL94_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBD4412549.1"
                     /translation="VALPDALERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMY
                     DQTFQRAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMI
                     YTHVLKVGGAGVRSPLDALPPLTSER"
BASE COUNT         1185 a         1663 c         1803 g         1298 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcacca ggaagctaaa atggagaaaa
       61 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      121 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      181 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      241 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      301 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      361 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      421 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      481 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
      541 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
      601 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
      661 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
      721 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
      781 tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta
      841 aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac
      901 cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat
      961 tgacgatatg atcatttatt ctgcctccca gagcctgata aaaacggtta gcgccggggt
     1021 tggatttttc agcgttccag ctaaggctaa ggcattcctg ttcaagcgca agccgggcta
     1081 taaagcgcat tatcgcttta cccgtgccct tatttcgatc gcactgagaa acatacagct
     1141 ctttaatatg cagctgaccg ctgtatcggg gcgagggata aagaatattg caacatgcca
     1201 ggccagtaat gttattgccg cagcgcgctc tgatcaccag ggtaccggaa agccggttga
     1261 ataacttttt acagagataa tccttcatca acgcttcctg aatgatacct tcgccataat
     1321 aggggtcgtc tcagaaaacg gaaaataaag cacgctaagc cggttgcaga ggccgtagcg
     1381 gcctgaactt ccccgcgccg atcttggcgc tgctgcgcca taggtaatca ccggtcaggt
     1441 tgatgtgctc ccagccgagt ggcgacaggt actgcaatag cgagtcatcg acggcatgac
     1501 cattgccgcg caacgcatgc gccgcacgct ccaggtagac cgtgttccac agcacgatgg
     1561 ccgccgtcac caggttgagg ccgctggccc ggtagcgctg ctgctcgaaa ctgcggtcac
     1621 ggatttcacc aaggcggttg aagaacacgg cacgggccag cgcattgcgc gcctcgccct
     1681 tgttcagccc ggcatgcacg cggcggcgta gctcgacgct ttgcagccag tcgaggatga
     1741 acagcgtgcg ctcgatgcgg cccaactcgc gcagcgcgac ggccaagccg ttctggcgcg
     1801 ggtagctgcc gagtttcctg agcatcagcg aggccgtcac cgtgccctgc ttgatcgagg
     1861 tggccagccg caggatttcg tcccaatggg cgcggacgtg cttgatgttg agcgtgccgc
     1921 cgatcatcgg cttgagcgcg tcataggcgg catcgccctt cgggatgtag agcttggtgt
     1981 cgcccaggtc gcggatgcgc ggcgcgaagc ggaagcccaa gaggtgcatc agggcgaaga
     2041 cgtgatcggt gaagcccgcc gtgtcggtgt agtgctcctc gatccgcagg tcggattcgt
     2101 ggtacagcag gccgtcgagc acgtaggttg agtcgcgcag gccgacattg accaccttgg
     2161 tgtggaatgg cgcgtattgg tcggagatgt gggtgtagaa agtccgtcct gggctgctgc
     2221 catattttgg gttgatgtgc cccgtgctct ttgccttgct agcggttcgg aaattctgtc
     2281 cgtccgatga tgatgtggtg ccatcgcccc agtgcccggc aaagggatgc cgaaactgag
     2341 cgttgaccag ttcagccaac gctgtcgagt acgtttcgtc gcgggtatgc caggcttgca
     2401 gccaagcgag cttcgcgtag gtcgtgccgg ggcaggactc ggccatcttg gtcaggccca
     2461 ggttgatcgc gtcggccagg atcgtggtca acaacaggtt cttgtccttg gccagatcgc
     2521 ccgatttcaa gtgcgtgaag tgccgggtga agcccgtcca ctcatcgact tcgagcagca
     2581 gttcggtgat cttgacgtgc ggcaggacca tggctgtctg gtctatcagc gcctgcgcgg
     2641 tgtcgggcac cgccgcatcc agcggcgtga tcttcaagcc cgactcggtg atgatggcat
     2701 ccggcaggtc gttggctgcc gccatgcggt tgacggtggc aagttgtgct tccagcagcg
     2761 tcagccgctc atgcagatat tgttcgcagt cggtggccac ggccagcggc aattcgctgg
     2821 actgcttgag gctggtgaac ttctcgggcg gtaccaggta gtcctcgaag tccttgaact
     2881 ggcgtgaacc ctgcacccag atgtcgcccg agcgcaggga gttcttcaac tcggacagcg
     2941 cgcacagttc gtagtagcgc cggtcgatgc cggcgtcggt catcaccagt ttctgccagc
     3001 gcggcttgat gaagccggtc ggtgcatcgg ctggcagctt gcgggcgttg tcggtgttca
     3061 tgccgcgcag cacctcaatg gcatcaagca cgtttttggc ggcgggcgcg gcccgcagct
     3121 tgagcacggc aaggaattcc ggtgcatagc ggcgcagggt ggcgtagctc tcgccgatgc
     3181 gatgcaggaa atcgaagtca tcgggttgcg cgagcttctg cgcctcggtg acgctctcgg
     3241 caaaggaatc ccaggacatg acggcctcga tggcggcaaa cgcatcgcgg cctgattgct
     3301 tggcgtcgat cagcgcctga ccgatgcgcc cgtacagacg taccttggcg ttgatggcct
     3361 tgcctgacgc ctggaactgc tgctgatgct tattcttggc agcgttaaac agcttaccca
     3421 ggatgcggtc gtgcaggtcg atgatttcgt cggtgacggt ggccatgccc tcggtggcca
     3481 gcgccacgag agtggcgtag cgccgttgcg gctcgaattt ggccaggtcg gcgggtgtca
     3541 tctggccgcc ctcgcgggca atcttgagca ggcggttctg gtgaaccagc cgctcgatgc
     3601 cggtaggcag atcgagtgcc tgccatgcct tgaggcgttc gatgtgttcc agcatatgcc
     3661 gcgaatttgg cttggccgga gactggcgca accaagccaa ccaggtcgtc ttgccgttgt
     3721 cccggcgctt gagcagatcg tcgaggcggc ggcgatgcgc gtccgccagt ggttcggcca
     3781 aggcgtcgta gatgcgccgg ttagcacggg tgatcgcctc ggcactcgcc cgctcgacgg
     3841 cgttgagggc gggcagaatg accgactgcc gccgcaggtg cccgatcaag gcgctggcca
     3901 gcacgatgcc tttgtcggtt tgcatcgcca gctcggtcag catctggacg gcctgccggt
     3961 aatggctcat ggtgaagggc cggaaaccga acacggtttg cagctcgctc aggtgctcgc
     4021 gccgggtctg ctcccgctgg ccgtactcgt tccagctttc gacgccgacc ttgagctggt
     4081 cggcgaccag cttcaacaag ggcgggaacg gtagttcatc gacgcccagg atgacgccgg
     4141 gaaagcgcag gtaacagagc tgcaccgcga agcccagccg attggctggc ccgcgccgct
     4201 gtcggatgat cgagaggtcg gtatcgttga atgtgtaatg tcggatcagg tcgtccttgg
     4261 agtccggcaa cgccagcagg ctttcccgct cggcggcgga caggatggaa cgacgtggca
     4321 tatttactga tccgttctca agtattgata cagggtttcg cgactgattc cgaattcacg
     4381 agcaagcttg gtcttttgct cgccagcctc gacacgttgg cgcagttcgg caatacgctc
     4441 agacgacagg gatttcttcc tgccacggta agccccgcgt tgcttggcga gcgcaatacc
     4501 ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg gcgaacgcgc ccatcaccga
     4561 gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa ctgaggtgtt ccttgacgaa
     4621 ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc cggcgcaaat catcgagatt
     4681 gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg ccggtgcggg cgaagcttat
     4741 cagcgcttcc agttgcggac gcttgacatc cttgccggat gccttgtcgc taaaagcgcg
     4801 atcaaccttg acgccttcca gttgccgttc cgggttctgg tcgaaggtgc tgaccctgat
     4861 atacccaatg cgctgtccag tcatggaatt ccctgcaaaa tgtcagggaa gactctatga
     4921 ccttcaacga gatatgtcaa taaattcaaa attcaatcct atcctgacgc aatttacaca
     4981 tggcatctga catcaggtta gggtatgcct caacctgacg gcggcgaatc acaagcgtcc
     5041 ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc
     5101 agcccgcaga aatgctccac gtactcggcc ccttccggcg tgaaggcggc atcgagcggg
     5161 atttccttgc agcacacgca gcaactggtg ctcggttcat tggcgttcat ggtggtgttc
     5221 ctccattggt tgacgaagcc gacgaaggcc gccgccggca tcggcctggc gaacaggaaa
     5281 ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca
     5341 ccctcggcta ccacctccat tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     5401 gactgcacta tagcagcgga ggggttggat ccatcaggca acgacgggct gctgccggcc
     5461 atcagcggac gcagggagga ctttccgcaa ccggccgttc gatgcggcac cgatggcctt
     5521 cgcgcagggg tagtgaatcc gccaggattg acttgcgctg ccctacctct cactagtgag
     5581 gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg ccaactttca gcacatgcgt
     5641 gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc
     5701 gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg
     5761 cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg cgctgaaagg tctggtcata
     5821 catgtgatgg cgacgcacga caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa
     5881 aacccagaac cacggccagg aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg
     5941 aagcgcaac
//
LOCUS       DAEBXD010000388         4777 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.205, whole genome shotgun sequence.
ACCESSION   DAEBXD010000388 DAEBXD010000000
VERSION     DAEBXD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.205"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(62..238)
                     /locus_tag="KJL94_004526"
     CDS             complement(62..238)
                     /locus_tag="KJL94_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD4412550.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            568..1383
                     /gene="sul2"
                     /locus_tag="KJL94_004527"
     CDS             568..1383
                     /gene="sul2"
                     /locus_tag="KJL94_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBD4412551.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1444..2247
                     /locus_tag="KJL94_004528"
                     /note="aph(3'')-Ib"
     CDS             1444..2247
                     /locus_tag="KJL94_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBD4412552.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2247..3083
                     /locus_tag="KJL94_004529"
     CDS             2247..3083
                     /locus_tag="KJL94_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD4412553.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3190..3594)
                     /locus_tag="KJL94_004530"
                     /pseudo
     CDS             complement(3190..3594)
                     /locus_tag="KJL94_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3804..4664)
                     /locus_tag="KJL94_004531"
     CDS             complement(3804..4664)
                     /locus_tag="KJL94_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBD4412554.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1063 a         1289 c         1254 g         1171 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgcca ggcgcgggaa taaaatccca
       61 ttcagacgcc ggatagattc aagcaagcca acttgtcgtc aaaatcggtg ttgcaaaaac
      121 gggagtgacc atagattccg ttttctgagg cgacccccac atagagacct tcccggtcat
      181 tcactttgta gagtttgtcc ctgggcttga gattgcgcag cttggtatcg gtcagcatgt
      241 accttgtcct tcttcgtctc cgatacccac tctattgtaa acaagacatt tttatctttt
      301 atattcaatg gcttattttc ctgctaattg gtaataccat gaaaaatacc atgctcagaa
      361 aaggcttaac aatattttga aaaattgcct actgagcgct gccgcacagc tccataggcc
      421 gctttcctgg ctttgcttcc agatgtatgc tattctgctc ctgcagctaa tggagcaccg
      481 caaacaggtt actcgcctgg ggattccctt tcgacccgag catccgtatg agactcatgc
      541 tcgattatta ttattataga agcccccatg aataaatcgc tcatcatttt cggcatcgtc
      601 aacataacct cggacagttt ctccgatgga ggccggtatc tggcgccaga cgcagccatt
      661 gcgcaggcgc gtaagctgat ggccgagggg gcagatgtga tcgacctcgg tccggcatcc
      721 agcaatcccg acgccgcgcc tgtttcgtcc gacacagaaa tcgcgcgtat cgcgccggtg
      781 ctggacgcgc tcaaggcaga tggcattccc gtctcgctcg acagttatca acccgcgacg
      841 caagcctatg ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg ttttccagac
      901 gctgcgttct atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat gcattcggtg
      961 caagacgggc aggcagatcg gcgcgaggca cccgctggcg acatcatgga tcacattgcg
     1021 gcgttctttg acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt
     1081 gtccttgatc ccggcatggg gttttttctg ggggctgctc ccgaaacctc gctctcggtg
     1141 ctggcgcggt tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct gtctgtttcg
     1201 cgcaaatcct ttctgcgcgc gctcacaggc cgtggtccgg gggatgtcgg ggccgcgaca
     1261 ctcgctgcag agcttgccgc cgccgcaggt ggagctgact tcatccgcac acacgagccg
     1321 cgccccttgc gcgacgggct ggcggtattg gcggcgctga aagaaaccgc aagaattcgt
     1381 taactgcaca ttcgggatat ttctctatat tcgcgcttca tcagaaaact gaaggaacct
     1441 ccattgaatc gaactaatat tttttttggt gaatcgcatt ctgactggtt gcctgtcaga
     1501 ggcggagaat ctggtgattt tgtttttcga cgtggtgacg ggcatgcctt cgcgaaaatc
     1561 gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg accgcctcat ttggctcaaa
     1621 ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg aggaacagga gggtgcatgc
     1681 ttggtgataa cggcaattcc gggagtaccg gcggctgatc tgtctggagc ggatttgctc
     1741 aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc acagcctatc ggttgatcaa
     1801 tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt ggtgtcccgc
     1861 aatgccgtca atcccgactt cttaccggac gaggacaaga gtacgccgca gctcgatctt
     1921 ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc aagagcgcac cgatatggtt
     1981 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2041 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2101 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2161 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2221 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     2281 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     2341 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     2401 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     2461 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     2521 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     2581 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     2641 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     2701 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     2761 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     2821 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     2881 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     2941 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3001 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3061 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3121 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3181 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3241 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     3301 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     3361 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     3421 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     3481 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     3541 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     3601 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttagggg
     3661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4081 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     4141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4261 aagttggcag cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcacca
     4441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4621 gcaaaaaagg gaataagggc gacacgaaaa tgttgaatac tcatactctt cctttttcaa
     4681 tattattgaa gcatttacca gggttattgt ctcatgagcg gatacatatt tgaatgtagg
     4741 cactgttgca aagttagcga tgaggcagcc ttttgtc
//
LOCUS       DAEBXD010000389         3757 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.225, whole genome shotgun sequence.
ACCESSION   DAEBXD010000389 DAEBXD010000000
VERSION     DAEBXD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.225"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            53..439
                     /locus_tag="KJL94_004532"
     CDS             53..439
                     /locus_tag="KJL94_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBD4412555.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            447..1133
                     /locus_tag="KJL94_004533"
     CDS             447..1133
                     /locus_tag="KJL94_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD4412556.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1111..1734)
                     /gene="tetR(B)"
                     /locus_tag="KJL94_004534"
     CDS             complement(1111..1734)
                     /gene="tetR(B)"
                     /locus_tag="KJL94_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBD4412557.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1816..3021
                     /gene="tet(B)"
                     /locus_tag="KJL94_004535"
     CDS             1816..3021
                     /gene="tet(B)"
                     /locus_tag="KJL94_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBD4412558.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3134..3727)
                     /gene="tetC"
                     /locus_tag="KJL94_004536"
     CDS             complement(3134..3727)
                     /gene="tetC"
                     /locus_tag="KJL94_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBD4412559.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT         1036 a          690 c          799 g         1232 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aggacaagga taatgtttga
       61 tgttcacgtt gttttagata atcaaatagg acaattagca ttactaggaa aaacattagg
      121 taataaaggt attggattgg aagggggagg gatatttacg gttggtgatg aatgccatgc
      181 tcattttctt gttgaacaag gaaaggaagc taaaatagcg ctagagcaag ctggactgtt
      241 agtacttgcg atccggacac cattaattcg taagttaaaa caggaaaaac cgggggaact
      301 tggcgaaata gcacgagtat tggcggagaa taacattaat attttagtgc aatacagtga
      361 ccatgctaac caactgatat taataacgga caatgatagt atggctgcat ctgttacgct
      421 cccttgggca ataaagtgaa cttgcgatgg ctaatttaat acgaaaagag gttacctttg
      481 agtcctcaat agccgcgata ggggcggcta tgtctgacat ttcacgagtt aaaatactca
      541 gtgctttgat ggatgggcga gcttggacgg ccactgagct aagttctgtg gcgaatatat
      601 cagcttcaac ggcgagcagt catttatcta aattattaga ttgccagcta atcacagtag
      661 tagctcaagg caagcatcgt tattttcggc tagcaggaaa agatattgct gaattgatgg
      721 aaagtatgat ggggatctcc ttaaaccatg gcgtacatgc caaagtttcc acgccagtgc
      781 atttacgaaa agcacgtact tgctatgatc atttagctgg cgaagttgcc gttaagatct
      841 atgattccct ttgtcaacag caatggatca ctgaaaatgg ttcaatgatc acattaagtg
      901 gtattcaata ttttcatgaa atgggaattg acgttccttc caaacattca cgtaaaatct
      961 gttgtgcgtg tttagattgg agtgaacgcc gtttccattt aggtgggtac gttggagccg
     1021 cattattttc gctttatgaa tctaaagggt ggttaactcg acatcttggt taccgtgaag
     1081 ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt taagttgttt
     1141 ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg
     1201 atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc
     1261 cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg
     1321 ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa
     1381 ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac
     1441 ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa
     1501 aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat
     1561 ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg
     1621 ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc
     1681 attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag acatcattaa
     1741 ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc cctatcagtg
     1801 atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat tggtaattac gttactcgat
     1861 gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga atttattgct
     1921 tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt aatgcaggtt
     1981 atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc agtgctgttg
     2041 ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag tgcgctttgg
     2101 atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc tgtcgcggca
     2161 tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg ttggttaggg
     2221 gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc aggagagatt
     2281 tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcgcttt ccttgtggtt
     2341 atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga agtaggggtt
     2401 gagacgcaat cgaattcggt atacatcact ttatttaaaa cgatgcccat tttgttgatt
     2461 atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt gctatttacc
     2521 gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg tcttggtctt
     2581 ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg gggcgaaaaa
     2641 acggcagtac tgctcggatt tattgcagat agtagtgcat ttgccttttt agcgtttata
     2701 tctgaaggtt ggttagtttt ccctgtttta attttattgg ctggtggtgg gatcgcttta
     2761 cctgcattac agggagtgat gtctatccaa acaaagagtc atcagcaagg tgctttacag
     2821 ggattattgg tgagccttac caatgcaacc ggtgttattg gcccattact gtttgctgtt
     2881 atttataatc attcactacc aatttgggat ggctggattt ggattattgg tttagcgttt
     2941 tactgtatta ttatcctgct atcgatgacc ttcatgttaa cccctcaagc tcaggggagt
     3001 aaacaggaga caagtgctta gttatttcgt caccaaatga tgttattccg cgaaatataa
     3061 tgaccctctt gataacccaa gagggcattt tttacgataa agaagattta gcttcaaata
     3121 aaacctatct attttattta tctttcaagc tcaataaaaa gccgcggtaa atagcaataa
     3181 attggccttt tttatcggca agctctttta ggtttttcgc atgtattgcg atatgcataa
     3241 accagccatt gagtaagttt ttaagcacat catcatcata agctttaagt tggttctctt
     3301 ggatcaattt gctgacaatg gcgtttacct taccagtaat gtattcaagg ctaatttttt
     3361 caagttcatt ccaaccaatg ataggcatca cttcttggat agggataagg tttttattat
     3421 tatcaataat ataatcaaga taatgttcaa atatactttc taaggcagac caaccatttg
     3481 ttaaatcagt ttttgttgtg atgtaggcat caatcataat taattgctgc ttataacagg
     3541 cactgagtaa ttgtttttta tttttaaagt gatgataaaa ggcacctttg gtcaccaacg
     3601 cttttcccga gatctcatct attgaaacag cttgatagcc tttttcaaca aacaatattc
     3661 gtgctgagtt aaccagtgat tgataggtac tcttaaaatt ttcttgttga tgatttttat
     3721 tttccatgat agatttaaaa taacataccg tcagtat
//
LOCUS       DAEBXD010000390         3740 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.227, whole genome shotgun sequence.
ACCESSION   DAEBXD010000390 DAEBXD010000000
VERSION     DAEBXD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.227"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            <1..762
                     /locus_tag="KJL94_004537"
     CDS             <1..762
                     /locus_tag="KJL94_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBD4412560.1"
                     /translation="PVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLG
                     SNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAK
                     IRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIEL
                     SHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYS
                     DVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            755..1489
                     /gene="frdB"
                     /locus_tag="KJL94_004538"
     CDS             755..1489
                     /gene="frdB"
                     /locus_tag="KJL94_004538"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD4412561.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1500..1895
                     /gene="frdC"
                     /locus_tag="KJL94_004539"
     CDS             1500..1895
                     /gene="frdC"
                     /locus_tag="KJL94_004539"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD4412562.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1906..2265
                     /gene="frdD"
                     /locus_tag="KJL94_004540"
     CDS             1906..2265
                     /gene="frdD"
                     /locus_tag="KJL94_004540"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD4412563.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2328..3461
                     /locus_tag="KJL94_004541"
     CDS             2328..3461
                     /locus_tag="KJL94_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD4412564.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3550..>3740
                     /gene="blc"
                     /locus_tag="KJL94_004542"
     CDS             3550..>3740
                     /gene="blc"
                     /locus_tag="KJL94_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD4412565.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTAT"
BASE COUNT          917 a          979 c          991 g          853 t
ORIGIN      
        1 ccggtacgtc cgactgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa
       61 acccgcatta aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca
      121 aaccgtctgg gctccaactc cctggcggaa ctggtggtct tcggccgtct ggccggtgaa
      181 caagcgacag agcgtgcagc aactgccggt aatggcaacg aagcggcaat tgaagcgcag
      241 gcagctggcg ttgaacaacg tctgaaagat ctagttaacc aggatggcgg cgaaaactgg
      301 gcgaagatcc gcgacgaaat gggcctggca atggaagaag gttgcggtat ctaccgtacg
      361 ccggaactga tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc
      421 gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg
      481 agccacggtc tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc
      541 cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caactttctc
      601 aaacacaccc tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg
      661 aagattacta cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat
      721 aaggcggaag cagccaataa taaggagaag gcgaatggct gagatgaaaa acctgaaaat
      781 tgaggtggtg cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt
      841 gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc
      901 accggacctg agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat
      961 ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat
     1021 gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca
     1081 cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga
     1141 tcagggtact aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg
     1201 catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat
     1261 cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa
     1321 gaaggagcgt atggcgcagt tgaacagcca gaacggtgta tggagctgta ctttcgtggg
     1381 ctattgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa
     1441 agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca
     1501 tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc
     1561 cgttttatcg cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca
     1621 ttgaactgat tttcgggctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg
     1681 tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg acggcagctc
     1741 tgctgcacac caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag
     1801 acgaaaaaat gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca
     1861 ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat
     1921 ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc
     1981 gcaattattg cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt
     2041 ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc
     2101 gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac
     2161 gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat gggttttcta cggtctggct
     2221 gctatcctga cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta
     2281 aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga
     2341 cgctctgcgc cttattaatt accgcctctt actccacatt tgctgcccct caacaaatca
     2401 acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg
     2461 cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg
     2521 ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat
     2581 ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg
     2641 caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc
     2701 ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa
     2761 gcgacttgct gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc
     2821 tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga
     2881 gttttgagca ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga
     2941 ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag
     3001 tgcatgtttc gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag
     3061 atatggcccg ctgggtgcaa agcaatttac ccccccttga tatcaatgag aaaacgcttc
     3121 aacaagggat acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc
     3181 tgggctggga aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca
     3241 ataaaattgc actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac
     3301 gcgcatcatg ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta
     3361 ttccagaaaa agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga
     3421 gagtcgccgc cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat
     3481 tttcggacct tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag
     3541 gaaacattta tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt
     3601 gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag tgaataattt cgacgccaaa
     3661 cgttatctcg gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg
     3721 gaaaaagtca ccgcaacata
//
LOCUS       DAEBXD010000391         3311 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.235, whole genome shotgun sequence.
ACCESSION   DAEBXD010000391 DAEBXD010000000
VERSION     DAEBXD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.235"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            109..1014
                     /locus_tag="KJL94_004543"
     CDS             109..1014
                     /locus_tag="KJL94_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBD4412566.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /locus_tag="KJL94_004544"
     CDS             1011..2249
                     /locus_tag="KJL94_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4412567.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..2833
                     /locus_tag="KJL94_004545"
     CDS             2249..2833
                     /locus_tag="KJL94_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4412568.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
BASE COUNT          550 a         1020 c         1063 g          678 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtggtgg aggggatccg
     2641 caagcgactg cccccaggtg ctcctgcggc agctgagttg ctcctgcact cggtcatcgc
     2701 tggcgcgacg atgcagtggg ccgtcgatcc ggatggtgag ctagctgatc atgtgctggc
     2761 tcagatcgct gccatcctgt gtttaatgtt tcccgaacac gacgatttcc aactcctcca
     2821 ggcacatgcg taaacggagg tgtgcagagt ccctgcggca ggcgacgaac acgaccgtcg
     2881 tcgattagta ccggtacggt cggtggtatc gaagtcttga tcaccactca ggtctacggc
     2941 ttacaaatgg tgaccatccc gatacttgcg tcagagcacc gggccgattc tttgacagtg
     3001 aatcactccc gtaaggttgt gccggtgtgg gtgtcccggg tcgagacgat actccgccaa
     3061 tgcgcccagc aaacaacctg gccatcgcag gtggtgggga gcggtgtggc ggatgagttg
     3121 gacaagttgg tgtagcagca cgagcacggc gagataacat cgcaggagtt cgacatgctc
     3181 aagagacagc tgattgcgaa tcgcgatgca gattcataac ccgattgcgg gttggcttca
     3241 ctccaccatc accgagcaga ctagcacggc gggctctgtt gcaaagattg gcggcagtca
     3301 gaggtaggct g
//
LOCUS       DAEBXD010000392         2949 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.247, whole genome shotgun sequence.
ACCESSION   DAEBXD010000392 DAEBXD010000000
VERSION     DAEBXD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.247"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            complement(<1..1371)
                     /gene="pepD"
                     /locus_tag="KJL94_004546"
     CDS             complement(<1..1371)
                     /gene="pepD"
                     /locus_tag="KJL94_004546"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD4412569.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPH"
     gene            1633..2091
                     /gene="gpt"
                     /locus_tag="KJL94_004547"
     CDS             1633..2091
                     /gene="gpt"
                     /locus_tag="KJL94_004547"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD4412570.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2183..>2949
                     /locus_tag="KJL94_004548"
     CDS             2183..>2949
                     /locus_tag="KJL94_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4412571.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKAL"
BASE COUNT          691 a          766 c          760 g          732 t
ORIGIN      
        1 gtgtggaccg gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt
       61 gaacagacca cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag
      121 gcgctgatag gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc
      181 aggatatgcg cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat
      241 gctcaccacg tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac
      301 gttattgtca gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc
      361 cacgtcggaa ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc
      421 gcgagatttc gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag
      481 atttttctct ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga
      541 tttcagtgcg tcgactttat cagctgcgac agcaatggtc gcaaaggctt cacgcgggat
      601 ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg
      661 acccgccagg aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc
      721 gccggagtga ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg
      781 aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat
      841 gtagatttca ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt
      901 gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc
      961 cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat
     1021 gccgttatcc gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg
     1081 ctggatagga tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc
     1141 gaggtgggcc tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg
     1201 aatcaggata ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc
     1261 aacaatgtat tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt
     1321 ggcaaaaata tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct
     1381 ccttgtcgat cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca
     1441 acacaagaca ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga
     1501 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     1561 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     1621 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     1681 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     1741 cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
     1801 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
     1861 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
     1921 gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
     1981 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa
     2041 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
     2101 cgcctggcat tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt
     2161 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
     2221 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
     2281 gcctgtgcag tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg
     2341 tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat
     2401 tgtgatgagc gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg
     2461 tggtggcaac tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg
     2521 tacggaagaa gatccgaaac tcagtggtcg acactggctg catgcggcta cgttgtacaa
     2581 cattgccgcc tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc
     2641 aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt
     2701 taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc
     2761 gttcccgaca gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct
     2821 gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt
     2881 gggcttttct tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt
     2941 aaaggcgct
//
LOCUS       DAEBXD010000393         1962 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.285, whole genome shotgun sequence.
ACCESSION   DAEBXD010000393 DAEBXD010000000
VERSION     DAEBXD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.285"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            109..939
                     /locus_tag="KJL94_004549"
     CDS             109..939
                     /locus_tag="KJL94_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBD4412572.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1052..1843
                     /gene="aadA1"
                     /locus_tag="KJL94_004550"
     CDS             1052..1843
                     /gene="aadA1"
                     /locus_tag="KJL94_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBD4412573.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          614 a          433 c          435 g          480 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga
      781 accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt
      841 tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag
      901 aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc
      961 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
     1021 tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc
     1081 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1141 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     1201 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1261 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1321 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     1381 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     1441 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     1501 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     1561 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1621 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1681 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1741 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1801 tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc
     1861 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     1921 tgcggtgatg ctgccaactt actgatttag tgtatgatgg tg
//
LOCUS       DAEBXD010000394         1016 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF195966
            SAMN17129038-rid12250783.guided.345, whole genome shotgun sequence.
ACCESSION   DAEBXD010000394 DAEBXD010000000
VERSION     DAEBXD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129038
            Sequence Read Archive: SRR13282545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042362.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:17:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,050
            CDSs (with protein)               :: 4,050
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 411
            CDSs (without protein)            :: 411
            Pseudo Genes (ambiguous residues) :: 0 of 411
            Pseudo Genes (frameshifted)       :: 183 of 411
            Pseudo Genes (incomplete)         :: 124 of 411
            Pseudo Genes (internal stop)      :: 153 of 411
            Pseudo Genes (multiple problems)  :: 46 of 411
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129038-rid12250783.guided.345"
                     /strain="SF195966"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-12"
                     /collected_by="LSPQ"
     gene            58..531
                     /locus_tag="KJL94_004551"
     CDS             58..531
                     /locus_tag="KJL94_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBD4412574.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            752..>1016
                     /gene="mobC"
                     /locus_tag="KJL94_004552"
     CDS             752..>1016
                     /gene="mobC"
                     /locus_tag="KJL94_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBD4412575.1"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
BASE COUNT          287 a          224 c          264 g          241 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccaggat gagaaccttg
       61 aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga ttggttgcgg tccagacata
      121 ccctggtccg cgaaagggga gcagctactt tttaaagcat tgacctacaa tcagtggctt
      181 ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc ccaataggaa atacgcggtc
      241 gttacccgct caggttggac atcaaatgat gacaatgtag ttgtatttca gtcaatcgaa
      301 gaggccatgg acaggctagc tgaattcacc ggtcacgtta tagtgtctgg tggcggagaa
      361 atttaccgag aaacattacc catggcctct acgctccact tatcgacgat cgacatcgag
      421 ccagaggggg atgttttctt cccgagtatt ccaaatacct tcgaagttgt ttttgagcaa
      481 cactttactt caaacattaa ctattgctat caaatttgga aaaagggtta acaaagctat
      541 gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac gccttttccg cgattgatag
      601 cgacgttatg tgaatattga aatgagcatt ccaaagaaag gaagtagaaa aattatcgtg
      661 ggcgaaaatg agtttttgtg gctcattagg tcaaaactca catattcgca ggactgttta
      721 ggtacagaaa tgactgcggt cgttgaaccg gatgccggtc ctcactgatg aagaagtgaa
      781 caccgtacgg gaatcgtgcc ggcagcttgg agctatcggc cggaacctca accaggtggc
      841 cagggccttg aacatcgagt tcagggaaag tgacaagctc aagcaagagg ccatcgaaaa
      901 actggccgaa cggatcgacc agcatttgga ccatgtgtct gagctgttcg ataagacctg
      961 gagccggtgg cacgattgtc attttcagaa gacgactgca ccagttgatt gggcgt
//
